CX28.8 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	CX28.8
Entrez Gene ID	566668
Full Name	connexin 28.8
Synonyms	si:busm1-189a20.5p,si:ch211-241j12.6p,zgc:136261
General protein information	Preferred Names connexin 28.8 Names gap junction beta-7 protein gap junction protein, beta 7, 25kDa
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001025544.2	NP_001020715.1	gap junction beta-7 protein
NM_001045239.2	NP_001038704.2	gap junction beta-7 protein
XM_009294485.2	XP_009292760.1	gap junction beta-7 protein isoform X1
XM_009294485.1	XP_009292760.1	gap junction beta-7 protein isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GJB7	XP_005627659.1
Bos taurus (cattle)	GJB7	XP_003586522.1
Danio rerio (zebrafish)	cx28.8	NP_001038704.2
Xenopus tropicalis (tropical clawed frog)	gjb7	XP_002933967.1
Homo sapiens (human)	GJB7	NP_940970.1
Pan troglodytes (chimpanzee)	GJB7	XP_001153611.1
	LOC100424941	XP_002803859.1

Gene Ontology
Bibliography

Related articles in PubMed

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

The connexin 30.3 of zebrafish homologue of human connexin 26 may play similar role in the inner ear.
Chang-Chien J, Yen YC, Chien KH, Li SY, Hsu TC, Yang JJ
Hearing research31355-66(2014 Jul)

Cx23, a connexin with only four extracellular-loop cysteines, forms functional gap junction channels and hemichannels.
Iovine MK, Gumpert AM, Falk MM, Mendelson TC
FEBS letters582(2)165-70(2008 Jan)

Evolutionary selection pressure and family relationships among connexin genes.
Cruciani V, Mikalsen SO
Biological chemistry388(3)253-64(2007 Mar)

Phylogenetic analysis of three complete gap junction gene families reveals lineage-specific duplications and highly supported gene classes.
Eastman SD, Chen TH, Falk MM, Mendelson TC, Iovine MK
Genomics87(2)265-74(2006 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following CX28.8 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the CX28.8 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa00405	NM_001025544.2 Latest version!	Danio rerio connexin 28.8 (cx28.8), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
ODa00405	NM_001045239.2 Latest version!	Danio rerio connexin 28.8 (cx28.8), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
ODa00405	XM_009294485.2 Latest version!	Danio rerio connexin 28.8 (cx28.8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
View Old Accession Versions >>
ODa00405	XM_009294485.1	Danio rerio connexin 28.8 (cx28.8), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

CX28.8 ( NM_001025544.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001020715.1 Danio rerio connexin 28.8 (cx28.8), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa00405
Clone ID Related Accession (Same CDS sequence)	NM_001025544.2 , NM_001045239.2 , XM_009294485.2 , XM_009294485.1
Accession Version	NM_001025544.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	gap junction beta-7 protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX511077.4. On Jun 12, 2009 this sequence version replaced NM_001025544.1. Transcript Variant: This variant (2) differs in the 5' UTR compared to variant 1. Both variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript is intronless :: BC163824.1 [ECO:0000345] ##Evidence-Data-END##

ATGAACTGGG GTTTCCTGGA GAACGTGTTG AGCGGGGTGA ACCGCTACTC CACCGTGGTG 
GGCCGGGTCT GGCTCTCCAT CCTCTTCATC TTCCGCATCC TGGTGTTCGT GGCGGCCGCC 
GAGCAGGTGT GGAAGGACGA GTTCAAGGAC TTCGTCTGCA ACACGCAGCA GCCGGGCTGC 
GAGCAGGTGT GCTTCGACCA CTTCTTCCCC ATCTCTCAGG TGCGTCTGTG GGCGCTGCAG 
CTCATCATGG TGTCCACGCC GTCGCTGCTG GTGGCTCTGC ACGTGGCCTA CCGAGAGCAC 
CGCGAGCGCA AACACAAGCG CAGGCTCTAC CAGGACAAGG GCAGCATTGA CGGCGGCCTG 
CTGTTCACGT ACATCACCAG CCTGGTCCTC AAGACGTCTT TCGAGGTGGG CACGCTGCTG 
GCCTTCTACC TGCTGTACAG CGGTTTCCAC GTGCCGCGGC TGCTGCGCTG CGCCGAGAGC 
CCCTGCCCCA ACAGCGTGGA CTGCTACATC GCCAGAGCCA CCGAGAAGAA GATCTTCCTC 
TACATCATGG GCTGCACCTC CATCCTGTGC ATCGCGCTCA ACCTGCTGGA GATGGGCTAC 
ATCGTGTCCA AGCAGTGCTG GAAGAGCTTC AGCAAGAGAT ACACTCCGGT GCGGGATGGG 
GCCACTCGCC CGCCCTCCAC CTTTACTCTC GGCCCACCGC AACCCTCATG CACAGCCAAG 
GAGGAGGGCG ATCAGTCTGC GCCGGCGGGA CAGGAGACAG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001020715.1
CDS	70..834
Translation	MNWGFLENVLSGVNRYSTVVGRVWLSILFIFRILVFVAAAEQVWKDEFKDFVCNTQQPGCEQVCFDHFFPISQVRLWALQLIMVSTPSLLVALHVAYREHRERKHKRRLYQDKGSIDGGLLFTYITSLVLKTSFEVGTLLAFYLLYSGFHVPRLLRCAESPCPNSVDCYIARATEKKIFLYIMGCTSILCIALNLLEMGYIVSKQCWKSFSKRYTPVRDGATRPPSTFTLGPPQPSCTAKEEGDQSAPAGQETA

Target ORF information:

RefSeq Version	NM_001025544.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio connexin 28.8 (cx28.8), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001025544.2

ORF Insert Sequence:

ATGAACTGGG GTTTCCTGGA GAACGTGTTG AGCGGGGTGA ACCGCTACTC CACCGTGGTG 
GGCCGGGTCT GGCTCTCCAT CCTCTTCATC TTCCGCATCC TGGTGTTCGT GGCGGCCGCC 
GAGCAGGTGT GGAAGGACGA GTTCAAGGAC TTCGTCTGCA ACACGCAGCA GCCGGGCTGC 
GAGCAGGTGT GCTTCGACCA CTTCTTCCCC ATCTCTCAGG TGCGTCTGTG GGCGCTGCAG 
CTCATCATGG TGTCCACGCC GTCGCTGCTG GTGGCTCTGC ACGTGGCCTA CCGAGAGCAC 
CGCGAGCGCA AACACAAGCG CAGGCTCTAC CAGGACAAGG GCAGCATTGA CGGCGGCCTG 
CTGTTCACGT ACATCACCAG CCTGGTCCTC AAGACGTCTT TCGAGGTGGG CACGCTGCTG 
GCCTTCTACC TGCTGTACAG CGGTTTCCAC GTGCCGCGGC TGCTGCGCTG CGCCGAGAGC 
CCCTGCCCCA ACAGCGTGGA CTGCTACATC GCCAGAGCCA CCGAGAAGAA GATCTTCCTC 
TACATCATGG GCTGCACCTC CATCCTGTGC ATCGCGCTCA ACCTGCTGGA GATGGGCTAC 
ATCGTGTCCA AGCAGTGCTG GAAGAGCTTC AGCAAGAGAT ACACTCCGGT GCGGGATGGG 
GCCACTCGCC CGCCCTCCAC CTTTACTCTC GGCCCACCGC AACCCTCATG CACAGCCAAG 
GAGGAGGGCG ATCAGTCTGC GCCGGCGGGA CAGGAGACAG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CX28.8 ( NM_001045239.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001038704.2 Danio rerio connexin 28.8 (cx28.8), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa00405
Clone ID Related Accession (Same CDS sequence)	NM_001025544.2 , NM_001045239.2 , XM_009294485.2 , XM_009294485.1
Accession Version	NM_001045239.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	gap junction beta-7 protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX511077.4. On Jun 12, 2009 this sequence version replaced NM_001045239.1. Transcript Variant: This variant (1) represents the longer transcript. Both variants 1 and 2 encode the same protein. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: CN020596.1, EB783922.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGAACTGGG GTTTCCTGGA GAACGTGTTG AGCGGGGTGA ACCGCTACTC CACCGTGGTG 
GGCCGGGTCT GGCTCTCCAT CCTCTTCATC TTCCGCATCC TGGTGTTCGT GGCGGCCGCC 
GAGCAGGTGT GGAAGGACGA GTTCAAGGAC TTCGTCTGCA ACACGCAGCA GCCGGGCTGC 
GAGCAGGTGT GCTTCGACCA CTTCTTCCCC ATCTCTCAGG TGCGTCTGTG GGCGCTGCAG 
CTCATCATGG TGTCCACGCC GTCGCTGCTG GTGGCTCTGC ACGTGGCCTA CCGAGAGCAC 
CGCGAGCGCA AACACAAGCG CAGGCTCTAC CAGGACAAGG GCAGCATTGA CGGCGGCCTG 
CTGTTCACGT ACATCACCAG CCTGGTCCTC AAGACGTCTT TCGAGGTGGG CACGCTGCTG 
GCCTTCTACC TGCTGTACAG CGGTTTCCAC GTGCCGCGGC TGCTGCGCTG CGCCGAGAGC 
CCCTGCCCCA ACAGCGTGGA CTGCTACATC GCCAGAGCCA CCGAGAAGAA GATCTTCCTC 
TACATCATGG GCTGCACCTC CATCCTGTGC ATCGCGCTCA ACCTGCTGGA GATGGGCTAC 
ATCGTGTCCA AGCAGTGCTG GAAGAGCTTC AGCAAGAGAT ACACTCCGGT GCGGGATGGG 
GCCACTCGCC CGCCCTCCAC CTTTACTCTC GGCCCACCGC AACCCTCATG CACAGCCAAG 
GAGGAGGGCG ATCAGTCTGC GCCGGCGGGA CAGGAGACAG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038704.2
CDS	177..941
Translation	MNWGFLENVLSGVNRYSTVVGRVWLSILFIFRILVFVAAAEQVWKDEFKDFVCNTQQPGCEQVCFDHFFPISQVRLWALQLIMVSTPSLLVALHVAYREHRERKHKRRLYQDKGSIDGGLLFTYITSLVLKTSFEVGTLLAFYLLYSGFHVPRLLRCAESPCPNSVDCYIARATEKKIFLYIMGCTSILCIALNLLEMGYIVSKQCWKSFSKRYTPVRDGATRPPSTFTLGPPQPSCTAKEEGDQSAPAGQETA

Target ORF information:

RefSeq Version	NM_001045239.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio connexin 28.8 (cx28.8), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001045239.2

ORF Insert Sequence:

ATGAACTGGG GTTTCCTGGA GAACGTGTTG AGCGGGGTGA ACCGCTACTC CACCGTGGTG 
GGCCGGGTCT GGCTCTCCAT CCTCTTCATC TTCCGCATCC TGGTGTTCGT GGCGGCCGCC 
GAGCAGGTGT GGAAGGACGA GTTCAAGGAC TTCGTCTGCA ACACGCAGCA GCCGGGCTGC 
GAGCAGGTGT GCTTCGACCA CTTCTTCCCC ATCTCTCAGG TGCGTCTGTG GGCGCTGCAG 
CTCATCATGG TGTCCACGCC GTCGCTGCTG GTGGCTCTGC ACGTGGCCTA CCGAGAGCAC 
CGCGAGCGCA AACACAAGCG CAGGCTCTAC CAGGACAAGG GCAGCATTGA CGGCGGCCTG 
CTGTTCACGT ACATCACCAG CCTGGTCCTC AAGACGTCTT TCGAGGTGGG CACGCTGCTG 
GCCTTCTACC TGCTGTACAG CGGTTTCCAC GTGCCGCGGC TGCTGCGCTG CGCCGAGAGC 
CCCTGCCCCA ACAGCGTGGA CTGCTACATC GCCAGAGCCA CCGAGAAGAA GATCTTCCTC 
TACATCATGG GCTGCACCTC CATCCTGTGC ATCGCGCTCA ACCTGCTGGA GATGGGCTAC 
ATCGTGTCCA AGCAGTGCTG GAAGAGCTTC AGCAAGAGAT ACACTCCGGT GCGGGATGGG 
GCCACTCGCC CGCCCTCCAC CTTTACTCTC GGCCCACCGC AACCCTCATG CACAGCCAAG 
GAGGAGGGCG ATCAGTCTGC GCCGGCGGGA CAGGAGACAG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CX28.8 ( XM_009294485.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009292760.1 Danio rerio connexin 28.8 (cx28.8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa00405
Clone ID Related Accession (Same CDS sequence)	NM_001025544.2 , NM_001045239.2 , XM_009294485.2 , XM_009294485.1
Accession Version	XM_009294485.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	gap junction beta-7 protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294485.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTGGG GTTTCCTGGA GAACGTGTTG AGCGGGGTGA ACCGCTACTC CACCGTGGTG 
GGCCGGGTCT GGCTCTCCAT CCTCTTCATC TTCCGCATCC TGGTGTTCGT GGCGGCCGCC 
GAGCAGGTGT GGAAGGACGA GTTCAAGGAC TTCGTCTGCA ACACGCAGCA GCCGGGCTGC 
GAGCAGGTGT GCTTCGACCA CTTCTTCCCC ATCTCTCAGG TGCGTCTGTG GGCGCTGCAG 
CTCATCATGG TGTCCACGCC GTCGCTGCTG GTGGCTCTGC ACGTGGCCTA CCGAGAGCAC 
CGCGAGCGCA AACACAAGCG CAGGCTCTAC CAGGACAAGG GCAGCATTGA CGGCGGCCTG 
CTGTTCACGT ACATCACCAG CCTGGTCCTC AAGACGTCTT TCGAGGTGGG CACGCTGCTG 
GCCTTCTACC TGCTGTACAG CGGTTTCCAC GTGCCGCGGC TGCTGCGCTG CGCCGAGAGC 
CCCTGCCCCA ACAGCGTGGA CTGCTACATC GCCAGAGCCA CCGAGAAGAA GATCTTCCTC 
TACATCATGG GCTGCACCTC CATCCTGTGC ATCGCGCTCA ACCTGCTGGA GATGGGCTAC 
ATCGTGTCCA AGCAGTGCTG GAAGAGCTTC AGCAAGAGAT ACACTCCGGT GCGGGATGGG 
GCCACTCGCC CGCCCTCCAC CTTTACTCTC GGCCCACCGC AACCCTCATG CACAGCCAAG 
GAGGAGGGCG ATCAGTCTGC GCCGGCGGGA CAGGAGACAG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292760.1
CDS	538..1302
Translation	MNWGFLENVLSGVNRYSTVVGRVWLSILFIFRILVFVAAAEQVWKDEFKDFVCNTQQPGCEQVCFDHFFPISQVRLWALQLIMVSTPSLLVALHVAYREHRERKHKRRLYQDKGSIDGGLLFTYITSLVLKTSFEVGTLLAFYLLYSGFHVPRLLRCAESPCPNSVDCYIARATEKKIFLYIMGCTSILCIALNLLEMGYIVSKQCWKSFSKRYTPVRDGATRPPSTFTLGPPQPSCTAKEEGDQSAPAGQETA

Target ORF information:

RefSeq Version	XM_009294485.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio connexin 28.8 (cx28.8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294485.2

ORF Insert Sequence:

ATGAACTGGG GTTTCCTGGA GAACGTGTTG AGCGGGGTGA ACCGCTACTC CACCGTGGTG 
GGCCGGGTCT GGCTCTCCAT CCTCTTCATC TTCCGCATCC TGGTGTTCGT GGCGGCCGCC 
GAGCAGGTGT GGAAGGACGA GTTCAAGGAC TTCGTCTGCA ACACGCAGCA GCCGGGCTGC 
GAGCAGGTGT GCTTCGACCA CTTCTTCCCC ATCTCTCAGG TGCGTCTGTG GGCGCTGCAG 
CTCATCATGG TGTCCACGCC GTCGCTGCTG GTGGCTCTGC ACGTGGCCTA CCGAGAGCAC 
CGCGAGCGCA AACACAAGCG CAGGCTCTAC CAGGACAAGG GCAGCATTGA CGGCGGCCTG 
CTGTTCACGT ACATCACCAG CCTGGTCCTC AAGACGTCTT TCGAGGTGGG CACGCTGCTG 
GCCTTCTACC TGCTGTACAG CGGTTTCCAC GTGCCGCGGC TGCTGCGCTG CGCCGAGAGC 
CCCTGCCCCA ACAGCGTGGA CTGCTACATC GCCAGAGCCA CCGAGAAGAA GATCTTCCTC 
TACATCATGG GCTGCACCTC CATCCTGTGC ATCGCGCTCA ACCTGCTGGA GATGGGCTAC 
ATCGTGTCCA AGCAGTGCTG GAAGAGCTTC AGCAAGAGAT ACACTCCGGT GCGGGATGGG 
GCCACTCGCC CGCCCTCCAC CTTTACTCTC GGCCCACCGC AACCCTCATG CACAGCCAAG 
GAGGAGGGCG ATCAGTCTGC GCCGGCGGGA CAGGAGACAG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

CX28.8 ( XM_009294485.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009292760.1 Danio rerio connexin 28.8 (cx28.8), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa00405
Clone ID Related Accession (Same CDS sequence)	NM_001025544.2 , NM_001045239.2 , XM_009294485.2 , XM_009294485.1
Accession Version	XM_009294485.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	gap junction beta-7 protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAACTGGG GTTTCCTGGA GAACGTGTTG AGCGGGGTGA ACCGCTACTC CACCGTGGTG 
GGCCGGGTCT GGCTCTCCAT CCTCTTCATC TTCCGCATCC TGGTGTTCGT GGCGGCCGCC 
GAGCAGGTGT GGAAGGACGA GTTCAAGGAC TTCGTCTGCA ACACGCAGCA GCCGGGCTGC 
GAGCAGGTGT GCTTCGACCA CTTCTTCCCC ATCTCTCAGG TGCGTCTGTG GGCGCTGCAG 
CTCATCATGG TGTCCACGCC GTCGCTGCTG GTGGCTCTGC ACGTGGCCTA CCGAGAGCAC 
CGCGAGCGCA AACACAAGCG CAGGCTCTAC CAGGACAAGG GCAGCATTGA CGGCGGCCTG 
CTGTTCACGT ACATCACCAG CCTGGTCCTC AAGACGTCTT TCGAGGTGGG CACGCTGCTG 
GCCTTCTACC TGCTGTACAG CGGTTTCCAC GTGCCGCGGC TGCTGCGCTG CGCCGAGAGC 
CCCTGCCCCA ACAGCGTGGA CTGCTACATC GCCAGAGCCA CCGAGAAGAA GATCTTCCTC 
TACATCATGG GCTGCACCTC CATCCTGTGC ATCGCGCTCA ACCTGCTGGA GATGGGCTAC 
ATCGTGTCCA AGCAGTGCTG GAAGAGCTTC AGCAAGAGAT ACACTCCGGT GCGGGATGGG 
GCCACTCGCC CGCCCTCCAC CTTTACTCTC GGCCCACCGC AACCCTCATG CACAGCCAAG 
GAGGAGGGCG ATCAGTCTGC GCCGGCGGGA CAGGAGACAG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292760.1
CDS	99..863
Translation	MNWGFLENVLSGVNRYSTVVGRVWLSILFIFRILVFVAAAEQVWKDEFKDFVCNTQQPGCEQVCFDHFFPISQVRLWALQLIMVSTPSLLVALHVAYREHRERKHKRRLYQDKGSIDGGLLFTYITSLVLKTSFEVGTLLAFYLLYSGFHVPRLLRCAESPCPNSVDCYIARATEKKIFLYIMGCTSILCIALNLLEMGYIVSKQCWKSFSKRYTPVRDGATRPPSTFTLGPPQPSCTAKEEGDQSAPAGQETA

Target ORF information:

RefSeq Version	XM_009294485.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio connexin 28.8 (cx28.8), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294485.1

ORF Insert Sequence:

ATGAACTGGG GTTTCCTGGA GAACGTGTTG AGCGGGGTGA ACCGCTACTC CACCGTGGTG 
GGCCGGGTCT GGCTCTCCAT CCTCTTCATC TTCCGCATCC TGGTGTTCGT GGCGGCCGCC 
GAGCAGGTGT GGAAGGACGA GTTCAAGGAC TTCGTCTGCA ACACGCAGCA GCCGGGCTGC 
GAGCAGGTGT GCTTCGACCA CTTCTTCCCC ATCTCTCAGG TGCGTCTGTG GGCGCTGCAG 
CTCATCATGG TGTCCACGCC GTCGCTGCTG GTGGCTCTGC ACGTGGCCTA CCGAGAGCAC 
CGCGAGCGCA AACACAAGCG CAGGCTCTAC CAGGACAAGG GCAGCATTGA CGGCGGCCTG 
CTGTTCACGT ACATCACCAG CCTGGTCCTC AAGACGTCTT TCGAGGTGGG CACGCTGCTG 
GCCTTCTACC TGCTGTACAG CGGTTTCCAC GTGCCGCGGC TGCTGCGCTG CGCCGAGAGC 
CCCTGCCCCA ACAGCGTGGA CTGCTACATC GCCAGAGCCA CCGAGAAGAA GATCTTCCTC 
TACATCATGG GCTGCACCTC CATCCTGTGC ATCGCGCTCA ACCTGCTGGA GATGGGCTAC 
ATCGTGTCCA AGCAGTGCTG GAAGAGCTTC AGCAAGAGAT ACACTCCGGT GCGGGATGGG 
GCCACTCGCC CGCCCTCCAC CTTTACTCTC GGCCCACCGC AACCCTCATG CACAGCCAAG 
GAGGAGGGCG ATCAGTCTGC GCCGGCGGGA CAGGAGACAG CCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

The connexin 30.3 of zebrafish homologue of human connexin 26 may play similar role in the inner ear.
Hearing research31355-66(2014 Jul)
Chang-Chien J,Yen YC,Chien KH,Li SY,Hsu TC,Yang JJ

Cx23, a connexin with only four extracellular-loop cysteines, forms functional gap junction channels and hemichannels.
FEBS letters582(2)165-70(2008 Jan)
Iovine MK,Gumpert AM,Falk MM,Mendelson TC

Evolutionary selection pressure and family relationships among connexin genes.
Biological chemistry388(3)253-64(2007 Mar)
Cruciani V,Mikalsen SO

Phylogenetic analysis of three complete gap junction gene families reveals lineage-specific duplications and highly supported gene classes.
Genomics87(2)265-74(2006 Feb)
Eastman SD,Chen TH,Falk MM,Mendelson TC,Iovine MK

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CX28.8 cDNA ORF clone, Danio rerio(Zebrafish)

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Target ORF information:

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Target ORF information:

ORF Insert Sequence:

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