c3b.2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	c3b.2
Entrez Gene ID	566347
Full Name	complement component c3b, tandem duplicate 2
Synonyms	si:ch211-140f21.1,wu:fc26b08
General protein information	Preferred Names complement component c3b, tandem duplicate 2 Names complement component c3b, tandem duplicate 2 c3.8 complement component 3-like component c3b, duplicate 2 component c3b, tandem duplicate 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005174032.2	XP_005174089.1	complement component c3b, tandem duplicate 2 isoform X2
XM_009296391.3	XP_009294666.1	complement component c3b, tandem duplicate 2 isoform X1
XM_009296391.2	XP_009294666.1	complement component c3b, tandem duplicate 2 isoform X1
NM_001114680.1	NP_001108152.1	complement component c3b, tandem duplicate 2 isoform 2 precursor
XM_005174032.3	XP_005174089.1	complement component c3b, tandem duplicate 2 isoform X2
NM_001100020.1	NP_001093490.1	complement component c3b, tandem duplicate 2 isoform 1 precursor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	si:ch211-140f21.1	NP_001093490.1
Danio rerio (zebrafish)	si:dkey-21e7.2	NP_001093483.1

Gene Ontology
Bibliography

Related articles in PubMed

The evolution and appearance of C3 duplications in fish originate an exclusive teleost c3 gene form with anti-inflammatory activity.
Forn-Cun? G, Reis ES, Dios S, Posada D, Lambris JD, Figueras A, Novoa B
PloS one9(6)e99673(2014)

Hematopoietic gene expression profile in zebrafish kidney marrow.
Song HD, Sun XJ, Deng M, Zhang GW, Zhou Y, Wu XY, Sheng Y, Chen Y, Ruan Z, Jiang CL, Fan HY, Zon LI, Kanki JP, Liu TX, Look AT, Chen Z
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following c3b.2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the c3b.2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa04992	NM_001114680.1 Latest version!	Danio rerio complement component c3b, tandem duplicate 2 (c3b.2), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$139.30 $199.00
ODa30637	NM_001100020.1 Latest version!	Danio rerio complement component c3b, tandem duplicate 2 (c3b.2), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa27026	XM_005174032.3 Latest version!	Danio rerio complement component c3b, tandem duplicate 2 (c3b.2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa44848	XM_009296391.3 Latest version!	Danio rerio complement component c3b, tandem duplicate 2 (c3b.2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
View Old Accession Versions >>
ODa44848	XM_009296391.2	Danio rerio complement component c3b, tandem duplicate 2 (c3b.2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
ODa27026	XM_005174032.2	Danio rerio complement component c3b, tandem duplicate 2 (c3b.2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$951.30 $1359.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

c3b.2 ( NM_001114680.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001108152.1 Danio rerio complement component c3b, tandem duplicate 2 (c3b.2), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa04992
Clone ID Related Accession (Same CDS sequence)	NM_001114680.1
Accession Version	NM_001114680.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 972bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Danio rerio(zebrafish)
Product	complement component c3b, tandem duplicate 2 isoform 2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC154024.1. ##Evidence-Data-START## Transcript exon combination :: BC154024.1 [ECO:0000332] ##Evidence-Data-END##

ATGCTTCTTC AGCTGCTGTT ATGGGCGACC CTGCTCTGTC AAATCTGCGA TGGAAAGGCC 
TTCCCTCAAC GACACAAGAG GACGGTTTGG GGACCTAATA TGCGACAAAT TGAGCCGGTC 
AAAGACCCTT CTCCGTCGTT TTCTCTTCTA GCTCCAGCAA AAATAAGGCC TGGAACAAAA 
CAGAACATCC TGGTGGAGGG ACACAACCTG GCAAAGGTGG TAAAAGTCAC TGTTGTGGTT 
TACGACTATC CAAACTCCCA AACTGCCTTG TCACGGGGCT CAGTTTCCCT CAATTCAGAC 
AACAACTACA GCGTCCTGCA AACCCTAGAG ATCAACCCAA ATTTGCTACT TCCAGATGGA 
AAAAAGAAGT ATGTGAAGCT TGTTGCTCAG TTCAGCAACA TCTATCGTGC AGAAAAAGTA 
ATGGCGGTGT CATTTCGCTC TGGACACATC TTCATTCAGA CCGACAAACC TATTTACAAC 
CCTGGAGACA AAGTGCGTTT CAGGGCTTTT GTGTCTGATT TGGAGTTTCG TTCTTCTGAG 
AGAACCATAT CTCTGGAGAT TCAGAATCCA GATGGAATTG CGGTGCATGG TATTGGGAGA 
GTTAAAGCAA TCGATGGGGT CTTTTCTGAC ATCTTCTCAC TGTCTACAGT AGTCAAGGAG 
GGCAGATGGA AAGTTGTAGC AAAGTTTGAT CAGGGAAAAG AGAATGTTTT CAGCAGAGAG 
TTTGAAGTGA AGAAATATGT GCTTCCAGCA TTCAACGTCA CACTTACTCC TAAAACATCC 
CACCTCAGTC TGGATGCAGA AAAGTTGGAA GTTGAAATAA CAGCCAGGTA TCTTTATGGC 
GCGCAAGTGA AGGGTGTTGC GTATGTGCTG TTTGGTATTG AAATTAATGG TGAAAAGAAG 
AGACTCACAT CTATGAAGCA AATGGTCAGT TTGGTTGAAG ATTACCAAAA CAATTTTTTT 
CCAGTATATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001108152.1
CDS	31..1002
Translation	MLLQLLLWATLLCQICDGKAFPQRHKRTVWGPNMRQIEPVKDPSPSFSLLAPAKIRPGTKQNILVEGHNLAKVVKVTVVVYDYPNSQTALSRGSVSLNSDNNYSVLQTLEINPNLLLPDGKKKYVKLVAQFSNIYRAEKVMAVSFRSGHIFIQTDKPIYNPGDKVRFRAFVSDLEFRSSERTISLEIQNPDGIAVHGIGRVKAIDGVFSDIFSLSTVVKEGRWKVVAKFDQGKENVFSREFEVKKYVLPAFNVTLTPKTSHLSLDAEKLEVEITARYLYGAQVKGVAYVLFGIEINGEKKRLTSMKQMVSLVEDYQNNFFPVY

Target ORF information:

RefSeq Version	NM_001114680.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio complement component c3b, tandem duplicate 2 (c3b.2), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001114680.1

ORF Insert Sequence:

ATGCTTCTTC AGCTGCTGTT ATGGGCGACC CTGCTCTGTC AAATCTGCGA TGGAAAGGCC 
TTCCCTCAAC GACACAAGAG GACGGTTTGG GGACCTAATA TGCGACAAAT TGAGCCGGTC 
AAAGACCCTT CTCCGTCGTT TTCTCTTCTA GCTCCAGCAA AAATAAGGCC TGGAACAAAA 
CAGAACATCC TGGTGGAGGG ACACAACCTG GCAAAGGTGG TAAAAGTCAC TGTTGTGGTT 
TACGACTATC CAAACTCCCA AACTGCCTTG TCACGGGGCT CAGTTTCCCT CAATTCAGAC 
AACAACTACA GCGTCCTGCA AACCCTAGAG ATCAACCCAA ATTTGCTACT TCCAGATGGA 
AAAAAGAAGT ATGTGAAGCT TGTTGCTCAG TTCAGCAACA TCTATCGTGC AGAAAAAGTA 
ATGGCGGTGT CATTTCGCTC TGGACACATC TTCATTCAGA CCGACAAACC TATTTACAAC 
CCTGGAGACA AAGTGCGTTT CAGGGCTTTT GTGTCTGATT TGGAGTTTCG TTCTTCTGAG 
AGAACCATAT CTCTGGAGAT TCAGAATCCA GATGGAATTG CGGTGCATGG TATTGGGAGA 
GTTAAAGCAA TCGATGGGGT CTTTTCTGAC ATCTTCTCAC TGTCTACAGT AGTCAAGGAG 
GGCAGATGGA AAGTTGTAGC AAAGTTTGAT CAGGGAAAAG AGAATGTTTT CAGCAGAGAG 
TTTGAAGTGA AGAAATATGT GCTTCCAGCA TTCAACGTCA CACTTACTCC TAAAACATCC 
CACCTCAGTC TGGATGCAGA AAAGTTGGAA GTTGAAATAA CAGCCAGGTA TCTTTATGGC 
GCGCAAGTGA AGGGTGTTGC GTATGTGCTG TTTGGTATTG AAATTAATGG TGAAAAGAAG 
AGACTCACAT CTATGAAGCA AATGGTCAGT TTGGTTGAAG ATTACCAAAA CAATTTTTTT 
CCAGTATATT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

c3b.2 ( NM_001100020.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001093490.1 Danio rerio complement component c3b, tandem duplicate 2 (c3b.2), transcript variant 1, mRNA.

Price & Availability↑

CloneID	ODa30637
Clone ID Related Accession (Same CDS sequence)	NM_001100020.1
Accession Version	NM_001100020.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5073bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Danio rerio(zebrafish)
Product	complement component c3b, tandem duplicate 2 isoform 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX649330.5. On Jul 26, 2007 this sequence version replaced XM_689619.2. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCTTCTTC AGCTGCTGTT ATGGGCGACC CTGCTCTGTC AAATCTGCGA TGGAAAGGCC 
TTCCCTCAAC GACACAAGAG GACGGTTTGG GGACCTAATA TGCGACAAAT TGAGCCGGTC 
AAAGACCCTT CTCCGTCGTT TTCTCTTCTA GCTCCAGCAA AAATAAGGCC TGGAACAAAA 
CAGAACATCC TGGTGGAGGG ACACAACCTG GCAAAGGTGG TAAAAGTCAC TGTTGTGGTT 
TACGACTATC CAAACTCCCA AACTGCCTTG TCACGGGGCT CAGTTTCCCT CAATTCAGAC 
AACAACTACA GCGTCCTGCA AACCCTAGAG ATCAACCCAA ATTTGCTACT TCCAGATGGA 
AAAAAGAAGT ATGTGAAGCT TGTTGCTCAG TTCAGCAACA TCTATCGTGC AGAAAAAGTA 
ATGGCGGTGT CATTTCGCTC TGGACACATC TTCATTCAGA CCGACAAACC TATTTACAAC 
CCTGGAGACA AAGTGCGTTT CAGGGCTTTT GTGTCTGATT TGGAGTTTCG TTCTTCTGAG 
AGAACCATAT CTCTGGAGAT TCAGAATCCA GATGGAATTG CGGTGCATGG TATTGGGAGA 
GTTAAAGCAA TCGATGGGGT CTTTTCTGAC ATCTTCTCAC TGTCTACAGT AGTCAAGGAG 
GGCAGATGGA AAGTTGTAGC AAAGTTTGAT CAGGGAAAAG AGAATGTTTT CAGCAGAGAG 
TTTGAAGTGA AGAAATATGT GCTTCCAGCA TTCAACGTCA CACTTACTCC TAAAACATCC 
CACCTCAGTC TGGATGCAGA AAAGTTGGAA GTTGAAATAA CAGCCAGGTA TCTTTATGGC 
GCGCAAGTGA AGGGTGTTGC GTATGTGCTG TTTGGTATTG AAATTAATGG TGAAAAGAAG 
AGACTCACAT CTATGAAGCA AATTGATGAT GTACTAAATG GAGGAAGTGT TAGTTTGACT 
ATGGAGGAGA TAAAGAGAGC TTATCCTGAC ATAAACAGCC TCCTGGGGTC TACGGTGTAC 
GTCAAAGCAT CAGTATTGAC TAGTTCAGGT AGCGATCTTG TGGAAGCAGA GAAATCCGGC 
ATTAAAATCG TAAATTCGCC CTACATGCTT ACCATCAAGG ACACACCAAA GTATTATAAA 
CCTGGTCTGC CGTTAGCCAT GACGGTTATC GTGAGTAACC ATGATGGATC TCCAACTCCT 
AATATTCCTG TAAAAGTCAC CTTTCTTAAA AGGCCAATCA GTGTCCACAG TGGCACCATC 
AACATTCACA TCAACATGCC AAAAAAGCGC ATCCCATACG GCAATTCCCA GATGTTGAAA 
GTGGAGACAG TGGATCCGTC ACTGAAACCA GAACAGCAGG CCATTCAAGA GGTATACGTC 
AAGCCGTATT CTACTTTTAG AAGTCACGGT AACTACTTGC ACGTCTCAGT AGGAGCAAGT 
CGAGTGGTGG TCGGTGGCAG TATCAGTGTC CAAGTGTACA TCAAATCCTC TTTACCAAAC 
CACAGAGCCC TCGTGGAGCA TCTCACATAC ATTGTGTTAA ATAAGGGGAA AATAATTCAG 
GCTGGTAGAG TGAATGTCAA AGGCCAGGAC GTAACAAGCG TTCATCTGCT CATTACCTCA 
GAAATGCTAC CTGCATTTCG GTTTGTGGCG TATTACGTTT TGCCATGGCA GCATAGGGCT 
GAAGTGGTGG CAGACTCAGT GTTTGTTGAT GTGGAGGACC GGTGTGTAGG TTCGCTTAAT 
GTTGGACCAG TGGATGGAGA AATAGTCAGT TCTTACTCAC CAGGCAGCAG CTTTAAATTT 
CAAGTGAAAG GTGATGTTGG AGCAAAGGTC AGCCTGGTAG CTGTGGATAA TGCCATCTTC 
CTGTTGAGTA AAAACCGCCT GACGCAGAAA AAGGTGTGGG AAGTGGTGGA TCAGGCTGAT 
ATGGGCTGCA CCGCAGGAGG AGGCAAGGAT AATATGGGTG TTTTCAGTGA TGCAGGACTG 
ATGTTTCACT CCAGCACAGG AGGTTCAACT GACTCCAAAG AAGACTGCTC CAGTAAAACT 
AAACGGCGAA GACGTTCTGC AGCCAAACTG CAGCTCAGAG ATCAGCTCGA ATATGATTAT 
ATGGAAGAAA AGGTTATATT GAGAGCGAGA ATGATAGAAC CCATGCCAAA GCTGTATGCC 
AGAGCTAATG ATGTCGGAGT TCGTCAGGAC AGTTATCGTG AAGTCGTTGT AGAAGAAGAG 
GAAGAAGAGG CGGCTTCAGT GTTTGAGGAA GATGATTTGT CTGATCTAGA AGATATTTAT 
GTGCGCAGCA AGTTTTTTGA GTCGTGGTTG TGGACCGATA TCAGACTGCC TAGCACTGCC 
AAGTCTGATG GGTTGGCTGT GTTTCCAGTG GACACCGTTC TTCCAGACAG TATCACACAG 
TGGGGGTTTC TAGCTGTCAG TGCCTCACCT GAGAAAGGTG TCTGCGTTGC TGAACCGTAT 
AACGTCCGCG TGTGGAAGCC GTTCTTCATC GACCTGCGTT TGCCTCACTC AGTGTCCAGA 
AATGAACAAG TGGAAATCAA AGCTGTAATC CACAACTACA AGAACTCAAC ACTGGAGGTT 
CGTTCCACAC CATCAGTAAA TGTTGTGATG GTGATCCTGG AGAAGACAGA GGACATGTGT 
AGTGTGGCCT ATACGGGACA GCACAGACAG CAGGTTACAG TAGCAGCCGG TTCCTCTAAA 
CTCATTACAT ACACCATCAT CCCTCTCAAA ACAGGAGAGC TTCCTCTACA GGTCACTGCT 
GTCGCTGCCT CATTCATGGG TCAAGATGCA GTGAAGAAGA ATCTACGCGT AGTAGTTGAA 
GGCATTCAGA CACTTAAAGT GAGGAGCTTT GTGTTGAACC CATCAGAGAA AGGAGGCAGT 
GCTGGGCAAC AGTTGATTCA AGTCAAGAAG CTGCAGTTGG ATGCTGTTGT GCCGAAATCT 
GTACCAGAAA CATTTGTCAA TGTCAGAGGT GATTTGCTGG CAGACAGTAT TGATAACTCC 
ATTAAGGAGG ACTCATTAGC AGCGTTGATC CGGATGCCCG GAGGCTGTGT GGAGCAGAAC 
CTGGCCAGAA TCACTCTGCC CCTCATCGCA GCTCATTACC TGGACCGCAG TGCAGACTGG 
GAGATTGTGG GAATCAGTCG CAGAGAAGAG GCCATCAAAT ACATACAGAA AGGTTATGAA 
AATCAACTTA ACTACAAAAA ATCAGACGAC TCGTACCCTC CCTACGCCAA TGAAGGCACC 
AGCACATGGA TCACTGCGTA TGTGGTGAAG GTTTTCTCCA TGGCGCATTC GTTCATTACC 
GTCAATGAGA AGCATCTGTG TGGCCCTCTG CTCTATTTAC TCAAGAATAA ACAGGGTTAT 
GATGGAACTT TTCAAGAGGA CAATCCTGTT TATGACACCA GTATGACGGG AGCTATGCGG 
GGATCTGAAT ACAAAGCATC TTTGACAGCA TTTGTACTCA TTGCTTTAGC AGAGGCACAA 
GATAAAGTTA CTTGCCAAGA TCAAGAAGTC AACATACGGG ATAAGTCTAG ACAGGCAGCA 
GGGTATCTGA CAGAGCATTT CTCCCGGTTG ACCAGGCCAT ATACAGCAGC TATAGCATGC 
TACGCGCTAG CCGTATCGAA CAACGCCTGC GTGAAGAGCA TGTTGCTCAA ATTTGCCTCA 
CCGGATCATT CCCACTGGCC TGATTCAAAC AATTATTTTT TCACACTGGA AGCTACAGGC 
TACGCACTGC TAGCGCTGAT CAAAAGTGGC CACATGGAAG AGGCAGAAGC TCCATTTCGA 
TGGCTGAATG AACACCGTGG CATTGGTGGA GGTTACGGCT CTACTCAGTC CACCATGGTG 
GTTCTTCAGG CCCTGTCTGA GTATCTAGTG AAGAGGCCTC CTCCGTCAGA CCTCAATTTG 
TTGGTTCAGC TCAGCGTTCC TGGTCGGAGT GATGTTCGCT GGACCTTCAA TCCCAATTTG 
GCTTATGTGG CCCGTTCTTC ACGAGTCCCT CTTGATCAGG AATTCTCAGT GGCGGCTTCT 
GGGAACGGCA AGGGTGTTTT GGAGGTGCTA AATGTCAATG TTTACCAAGA ATATCTTGAA 
CTAGAATATA AAAATCAAGA TATAATTTAT CGTTTATGTG TCACAGAAAA AACCTCATCG 
GATGTAGAAA AGTCCTTCAG ACTCAATGTC AATGTGAGGG CTCTGGAAGA ACGGCAAGTG 
AGGATGGTTA TTCTAGACAT CGGCCTGCCT ACAGGCTTTG AACCTGAAAA CTCTGATTTA 
GAGTTGCTCA CAAATTCAGT GGATCGCTAC ATCAACAACT TCCAAGTGGT GGACAACCTG 
AGCGACAGAG GATCCCTCAT TATTCACCTG TTCAAGGTGT CCAACAAAGA GGCAGAAATC 
ATCTCATTCA GACTCAATCA GAGGTTTAAA GTGGGGCTCC TTCAGCCTTC CACAGTCAAA 
GTTTACCAGT ACTACAACAA AGATAAAAGC TGTAGCAGGT TCTACTCTCC TCCTGAAGAC 
AAACAGCAGC TGGATCAGAT CTGTGAAGGC GACGTGTGCC GCTGCTCGCA GGGAGACTGT 
TGTGTAATGA AGGAAGACTC CGCGTCTTTT GGTAAAGAGC AGAGGAATGC CGCTGTATGC 
AACGGATTAC ATCATGGTGA ATATTTCTTT GGGTCTAATT ACAGTGGTTT ATTATTCCTG 
TCAATGAACA ACTTTTCTTC AGCCTACAGA GTCAAGCTGA TCAGCATCAG CCAGAGTCAC 
TATTACCAGT ATGAGATGGA GATTGTGCAG GTTATTAAAG AAGGGACGGA GGAAGGTCTG 
AATGAGAAGG AGAGGAGGAT GTTCCTCTCT CATGCGAGCT GCAGGACCAG GCTTAATTTG 
AGAGAGGGTC AGGAGTATCT GGTCATTGGG CCGATCACTG ATGTTTGGAA TGCAGGAAGC 
ACAGTGTCCA ACAAGTATGT GTATACGCTT GGGAAAGAAA CGTGGGTTGA GCGCTGGCCA 
GATGAGTCTG AATGTGGATC AACACTGCAG AAGAAATGTG ACGAGCTGAA GAGCTTTGCA 
AAGAAACTGA TTGAAGACGG CTGTCAGGCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001093490.1
CDS	88..5160
Translation	MLLQLLLWATLLCQICDGKAFPQRHKRTVWGPNMRQIEPVKDPSPSFSLLAPAKIRPGTKQNILVEGHNLAKVVKVTVVVYDYPNSQTALSRGSVSLNSDNNYSVLQTLEINPNLLLPDGKKKYVKLVAQFSNIYRAEKVMAVSFRSGHIFIQTDKPIYNPGDKVRFRAFVSDLEFRSSERTISLEIQNPDGIAVHGIGRVKAIDGVFSDIFSLSTVVKEGRWKVVAKFDQGKENVFSREFEVKKYVLPAFNVTLTPKTSHLSLDAEKLEVEITARYLYGAQVKGVAYVLFGIEINGEKKRLTSMKQIDDVLNGGSVSLTMEEIKRAYPDINSLLGSTVYVKASVLTSSGSDLVEAEKSGIKIVNSPYMLTIKDTPKYYKPGLPLAMTVIVSNHDGSPTPNIPVKVTFLKRPISVHSGTINIHINMPKKRIPYGNSQMLKVETVDPSLKPEQQAIQEVYVKPYSTFRSHGNYLHVSVGASRVVVGGSISVQVYIKSSLPNHRALVEHLTYIVLNKGKIIQAGRVNVKGQDVTSVHLLITSEMLPAFRFVAYYVLPWQHRAEVVADSVFVDVEDRCVGSLNVGPVDGEIVSSYSPGSSFKFQVKGDVGAKVSLVAVDNAIFLLSKNRLTQKKVWEVVDQADMGCTAGGGKDNMGVFSDAGLMFHSSTGGSTDSKEDCSSKTKRRRRSAAKLQLRDQLEYDYMEEKVILRARMIEPMPKLYARANDVGVRQDSYREVVVEEEEEEAASVFEEDDLSDLEDIYVRSKFFESWLWTDIRLPSTAKSDGLAVFPVDTVLPDSITQWGFLAVSASPEKGVCVAEPYNVRVWKPFFIDLRLPHSVSRNEQVEIKAVIHNYKNSTLEVRSTPSVNVVMVILEKTEDMCSVAYTGQHRQQVTVAAGSSKLITYTIIPLKTGELPLQVTAVAASFMGQDAVKKNLRVVVEGIQTLKVRSFVLNPSEKGGSAGQQLIQVKKLQLDAVVPKSVPETFVNVRGDLLADSIDNSIKEDSLAALIRMPGGCVEQNLARITLPLIAAHYLDRSADWEIVGISRREEAIKYIQKGYENQLNYKKSDDSYPPYANEGTSTWITAYVVKVFSMAHSFITVNEKHLCGPLLYLLKNKQGYDGTFQEDNPVYDTSMTGAMRGSEYKASLTAFVLIALAEAQDKVTCQDQEVNIRDKSRQAAGYLTEHFSRLTRPYTAAIACYALAVSNNACVKSMLLKFASPDHSHWPDSNNYFFTLEATGYALLALIKSGHMEEAEAPFRWLNEHRGIGGGYGSTQSTMVVLQALSEYLVKRPPPSDLNLLVQLSVPGRSDVRWTFNPNLAYVARSSRVPLDQEFSVAASGNGKGVLEVLNVNVYQEYLELEYKNQDIIYRLCVTEKTSSDVEKSFRLNVNVRALEERQVRMVILDIGLPTGFEPENSDLELLTNSVDRYINNFQVVDNLSDRGSLIIHLFKVSNKEAEIISFRLNQRFKVGLLQPSTVKVYQYYNKDKSCSRFYSPPEDKQQLDQICEGDVCRCSQGDCCVMKEDSASFGKEQRNAAVCNGLHHGEYFFGSNYSGLLFLSMNNFSSAYRVKLISISQSHYYQYEMEIVQVIKEGTEEGLNEKERRMFLSHASCRTRLNLREGQEYLVIGPITDVWNAGSTVSNKYVYTLGKETWVERWPDESECGSTLQKKCDELKSFAKKLIEDGCQA

Target ORF information:

RefSeq Version	NM_001100020.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio complement component c3b, tandem duplicate 2 (c3b.2), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001100020.1

ORF Insert Sequence:

ATGCTTCTTC AGCTGCTGTT ATGGGCGACC CTGCTCTGTC AAATCTGCGA TGGAAAGGCC 
TTCCCTCAAC GACACAAGAG GACGGTTTGG GGACCTAATA TGCGACAAAT TGAGCCGGTC 
AAAGACCCTT CTCCGTCGTT TTCTCTTCTA GCTCCAGCAA AAATAAGGCC TGGAACAAAA 
CAGAACATCC TGGTGGAGGG ACACAACCTG GCAAAGGTGG TAAAAGTCAC TGTTGTGGTT 
TACGACTATC CAAACTCCCA AACTGCCTTG TCACGGGGCT CAGTTTCCCT CAATTCAGAC 
AACAACTACA GCGTCCTGCA AACCCTAGAG ATCAACCCAA ATTTGCTACT TCCAGATGGA 
AAAAAGAAGT ATGTGAAGCT TGTTGCTCAG TTCAGCAACA TCTATCGTGC AGAAAAAGTA 
ATGGCGGTGT CATTTCGCTC TGGACACATC TTCATTCAGA CCGACAAACC TATTTACAAC 
CCTGGAGACA AAGTGCGTTT CAGGGCTTTT GTGTCTGATT TGGAGTTTCG TTCTTCTGAG 
AGAACCATAT CTCTGGAGAT TCAGAATCCA GATGGAATTG CGGTGCATGG TATTGGGAGA 
GTTAAAGCAA TCGATGGGGT CTTTTCTGAC ATCTTCTCAC TGTCTACAGT AGTCAAGGAG 
GGCAGATGGA AAGTTGTAGC AAAGTTTGAT CAGGGAAAAG AGAATGTTTT CAGCAGAGAG 
TTTGAAGTGA AGAAATATGT GCTTCCAGCA TTCAACGTCA CACTTACTCC TAAAACATCC 
CACCTCAGTC TGGATGCAGA AAAGTTGGAA GTTGAAATAA CAGCCAGGTA TCTTTATGGC 
GCGCAAGTGA AGGGTGTTGC GTATGTGCTG TTTGGTATTG AAATTAATGG TGAAAAGAAG 
AGACTCACAT CTATGAAGCA AATTGATGAT GTACTAAATG GAGGAAGTGT TAGTTTGACT 
ATGGAGGAGA TAAAGAGAGC TTATCCTGAC ATAAACAGCC TCCTGGGGTC TACGGTGTAC 
GTCAAAGCAT CAGTATTGAC TAGTTCAGGT AGCGATCTTG TGGAAGCAGA GAAATCCGGC 
ATTAAAATCG TAAATTCGCC CTACATGCTT ACCATCAAGG ACACACCAAA GTATTATAAA 
CCTGGTCTGC CGTTAGCCAT GACGGTTATC GTGAGTAACC ATGATGGATC TCCAACTCCT 
AATATTCCTG TAAAAGTCAC CTTTCTTAAA AGGCCAATCA GTGTCCACAG TGGCACCATC 
AACATTCACA TCAACATGCC AAAAAAGCGC ATCCCATACG GCAATTCCCA GATGTTGAAA 
GTGGAGACAG TGGATCCGTC ACTGAAACCA GAACAGCAGG CCATTCAAGA GGTATACGTC 
AAGCCGTATT CTACTTTTAG AAGTCACGGT AACTACTTGC ACGTCTCAGT AGGAGCAAGT 
CGAGTGGTGG TCGGTGGCAG TATCAGTGTC CAAGTGTACA TCAAATCCTC TTTACCAAAC 
CACAGAGCCC TCGTGGAGCA TCTCACATAC ATTGTGTTAA ATAAGGGGAA AATAATTCAG 
GCTGGTAGAG TGAATGTCAA AGGCCAGGAC GTAACAAGCG TTCATCTGCT CATTACCTCA 
GAAATGCTAC CTGCATTTCG GTTTGTGGCG TATTACGTTT TGCCATGGCA GCATAGGGCT 
GAAGTGGTGG CAGACTCAGT GTTTGTTGAT GTGGAGGACC GGTGTGTAGG TTCGCTTAAT 
GTTGGACCAG TGGATGGAGA AATAGTCAGT TCTTACTCAC CAGGCAGCAG CTTTAAATTT 
CAAGTGAAAG GTGATGTTGG AGCAAAGGTC AGCCTGGTAG CTGTGGATAA TGCCATCTTC 
CTGTTGAGTA AAAACCGCCT GACGCAGAAA AAGGTGTGGG AAGTGGTGGA TCAGGCTGAT 
ATGGGCTGCA CCGCAGGAGG AGGCAAGGAT AATATGGGTG TTTTCAGTGA TGCAGGACTG 
ATGTTTCACT CCAGCACAGG AGGTTCAACT GACTCCAAAG AAGACTGCTC CAGTAAAACT 
AAACGGCGAA GACGTTCTGC AGCCAAACTG CAGCTCAGAG ATCAGCTCGA ATATGATTAT 
ATGGAAGAAA AGGTTATATT GAGAGCGAGA ATGATAGAAC CCATGCCAAA GCTGTATGCC 
AGAGCTAATG ATGTCGGAGT TCGTCAGGAC AGTTATCGTG AAGTCGTTGT AGAAGAAGAG 
GAAGAAGAGG CGGCTTCAGT GTTTGAGGAA GATGATTTGT CTGATCTAGA AGATATTTAT 
GTGCGCAGCA AGTTTTTTGA GTCGTGGTTG TGGACCGATA TCAGACTGCC TAGCACTGCC 
AAGTCTGATG GGTTGGCTGT GTTTCCAGTG GACACCGTTC TTCCAGACAG TATCACACAG 
TGGGGGTTTC TAGCTGTCAG TGCCTCACCT GAGAAAGGTG TCTGCGTTGC TGAACCGTAT 
AACGTCCGCG TGTGGAAGCC GTTCTTCATC GACCTGCGTT TGCCTCACTC AGTGTCCAGA 
AATGAACAAG TGGAAATCAA AGCTGTAATC CACAACTACA AGAACTCAAC ACTGGAGGTT 
CGTTCCACAC CATCAGTAAA TGTTGTGATG GTGATCCTGG AGAAGACAGA GGACATGTGT 
AGTGTGGCCT ATACGGGACA GCACAGACAG CAGGTTACAG TAGCAGCCGG TTCCTCTAAA 
CTCATTACAT ACACCATCAT CCCTCTCAAA ACAGGAGAGC TTCCTCTACA GGTCACTGCT 
GTCGCTGCCT CATTCATGGG TCAAGATGCA GTGAAGAAGA ATCTACGCGT AGTAGTTGAA 
GGCATTCAGA CACTTAAAGT GAGGAGCTTT GTGTTGAACC CATCAGAGAA AGGAGGCAGT 
GCTGGGCAAC AGTTGATTCA AGTCAAGAAG CTGCAGTTGG ATGCTGTTGT GCCGAAATCT 
GTACCAGAAA CATTTGTCAA TGTCAGAGGT GATTTGCTGG CAGACAGTAT TGATAACTCC 
ATTAAGGAGG ACTCATTAGC AGCGTTGATC CGGATGCCCG GAGGCTGTGT GGAGCAGAAC 
CTGGCCAGAA TCACTCTGCC CCTCATCGCA GCTCATTACC TGGACCGCAG TGCAGACTGG 
GAGATTGTGG GAATCAGTCG CAGAGAAGAG GCCATCAAAT ACATACAGAA AGGTTATGAA 
AATCAACTTA ACTACAAAAA ATCAGACGAC TCGTACCCTC CCTACGCCAA TGAAGGCACC 
AGCACATGGA TCACTGCGTA TGTGGTGAAG GTTTTCTCCA TGGCGCATTC GTTCATTACC 
GTCAATGAGA AGCATCTGTG TGGCCCTCTG CTCTATTTAC TCAAGAATAA ACAGGGTTAT 
GATGGAACTT TTCAAGAGGA CAATCCTGTT TATGACACCA GTATGACGGG AGCTATGCGG 
GGATCTGAAT ACAAAGCATC TTTGACAGCA TTTGTACTCA TTGCTTTAGC AGAGGCACAA 
GATAAAGTTA CTTGCCAAGA TCAAGAAGTC AACATACGGG ATAAGTCTAG ACAGGCAGCA 
GGGTATCTGA CAGAGCATTT CTCCCGGTTG ACCAGGCCAT ATACAGCAGC TATAGCATGC 
TACGCGCTAG CCGTATCGAA CAACGCCTGC GTGAAGAGCA TGTTGCTCAA ATTTGCCTCA 
CCGGATCATT CCCACTGGCC TGATTCAAAC AATTATTTTT TCACACTGGA AGCTACAGGC 
TACGCACTGC TAGCGCTGAT CAAAAGTGGC CACATGGAAG AGGCAGAAGC TCCATTTCGA 
TGGCTGAATG AACACCGTGG CATTGGTGGA GGTTACGGCT CTACTCAGTC CACCATGGTG 
GTTCTTCAGG CCCTGTCTGA GTATCTAGTG AAGAGGCCTC CTCCGTCAGA CCTCAATTTG 
TTGGTTCAGC TCAGCGTTCC TGGTCGGAGT GATGTTCGCT GGACCTTCAA TCCCAATTTG 
GCTTATGTGG CCCGTTCTTC ACGAGTCCCT CTTGATCAGG AATTCTCAGT GGCGGCTTCT 
GGGAACGGCA AGGGTGTTTT GGAGGTGCTA AATGTCAATG TTTACCAAGA ATATCTTGAA 
CTAGAATATA AAAATCAAGA TATAATTTAT CGTTTATGTG TCACAGAAAA AACCTCATCG 
GATGTAGAAA AGTCCTTCAG ACTCAATGTC AATGTGAGGG CTCTGGAAGA ACGGCAAGTG 
AGGATGGTTA TTCTAGACAT CGGCCTGCCT ACAGGCTTTG AACCTGAAAA CTCTGATTTA 
GAGTTGCTCA CAAATTCAGT GGATCGCTAC ATCAACAACT TCCAAGTGGT GGACAACCTG 
AGCGACAGAG GATCCCTCAT TATTCACCTG TTCAAGGTGT CCAACAAAGA GGCAGAAATC 
ATCTCATTCA GACTCAATCA GAGGTTTAAA GTGGGGCTCC TTCAGCCTTC CACAGTCAAA 
GTTTACCAGT ACTACAACAA AGATAAAAGC TGTAGCAGGT TCTACTCTCC TCCTGAAGAC 
AAACAGCAGC TGGATCAGAT CTGTGAAGGC GACGTGTGCC GCTGCTCGCA GGGAGACTGT 
TGTGTAATGA AGGAAGACTC CGCGTCTTTT GGTAAAGAGC AGAGGAATGC CGCTGTATGC 
AACGGATTAC ATCATGGTGA ATATTTCTTT GGGTCTAATT ACAGTGGTTT ATTATTCCTG 
TCAATGAACA ACTTTTCTTC AGCCTACAGA GTCAAGCTGA TCAGCATCAG CCAGAGTCAC 
TATTACCAGT ATGAGATGGA GATTGTGCAG GTTATTAAAG AAGGGACGGA GGAAGGTCTG 
AATGAGAAGG AGAGGAGGAT GTTCCTCTCT CATGCGAGCT GCAGGACCAG GCTTAATTTG 
AGAGAGGGTC AGGAGTATCT GGTCATTGGG CCGATCACTG ATGTTTGGAA TGCAGGAAGC 
ACAGTGTCCA ACAAGTATGT GTATACGCTT GGGAAAGAAA CGTGGGTTGA GCGCTGGCCA 
GATGAGTCTG AATGTGGATC AACACTGCAG AAGAAATGTG ACGAGCTGAA GAGCTTTGCA 
AAGAAACTGA TTGAAGACGG CTGTCAGGCT TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

c3b.2 ( XM_005174032.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005174089.1 Danio rerio complement component c3b, tandem duplicate 2 (c3b.2), transcript variant X2, mRNA.

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CloneID	ODa27026
Clone ID Related Accession (Same CDS sequence)	XM_005174032.2 , XM_005174032.3
Accession Version	XM_005174032.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	complement component c3b, tandem duplicate 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_005174032.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCTTC AGCTGCTGTT ATGGGCGACC CTGCTCTGTC AAATCTGCGA TGGAAAGGCC 
TTCCCTCAAC GACACAAGAG GACGGTTTGG GGACCTAATA TGCGACAAAT TGAGCCGGTC 
AAAGACCCTT CTCCGTCGTT TTCTCTTCTA GCTCCAGCAA AAATAAGGCC TGGAACAAAA 
CAGAACATCC TGGTGGAGGG ACACAACCTG GCAAAGGTGG TAAAAGTCAC TGTTGTGGTT 
TACGACTATC CAAACTCCCA AACTGCCTTG TCACGGGGCT CAGTTTCCCT CAATTCAGAC 
AACAACTACA GCGTCCTGCA AACCCTAGAG ATCAACCCAA ATTTGCTACT TCCAGATGGA 
AAAAAGAAGT ATGTGAAGCT TGTTGCTCAG TTCAGCAACA TCTATCGTGC AGAAAAAGTA 
ATGGCGGTGT CATTTCGCTC TGGACACATC TTCATTCAGA CCGACAAACC TATTTACAAC 
CCTGGAGACA AAGTGCGTTT CAGGGCTTTT GTGTCTGATT TGGAGTTTCG TTCTTCTGAG 
AGAACCATAT CTCTGGAGAT TCAGAATCCA GATGGAATTG CGGTGCATGG TATTGGGAGA 
GTTAAAGCAA TCGATGGGGT CTTTTCTGAC ATCTTCTCAC TGTCTACAGT AGTCAAGGAG 
GGCAGATGGA AAGTTGTAGC AAAGTTTGAT CAGGGAAAAG AGAATGTTTT CAGCAGAGAG 
TTTGAAGTGA AGAAATATGT GCTTCCAGCA TTCAACGTCA CACTTACTCC TAAAACATCC 
CACCTCAGTC TGGATGCAGA AAAGTTGGAA GTTGAAATAA CAGCCAGGTA TCTTTATGGC 
GCGCAAGTGA AGGGTGTTGC GTATGTGCTG TTTGGTATTG AAATTAATGG TGAAAAGAAG 
AGACTCACAT CTATGAAGCA AATTGATGAT CTAAATGGAG GAAGTGTTAG TTTGACTATG 
GAGGAGATAA AGAGAGCTTA TCCTGACATA AACAGCCTCC TGGGGTCTAC GGTGTACGTC 
AAAGCATCAG TATTGACTAG TTCAGGTAGC GATCTTGTGG AAGCAGAGAA ATCCGGCATT 
AAAATCGTAA ATTCGCCCTA CATGCTTACC ATCAAGGACA CACCAAAGTA TTATAAACCT 
GGTCTGCCGT TAGCCATGAC GGTTATCGTG AGTAACCATG ATGGATCTCC AACTCCTAAT 
ATTCCTGTAA AAGTCACCTT TCTTAAAAGG CCAATCAGTG TCCACAGTGG CACCATCAAC 
ATTCACATCA ACATGCCAAA AAAGCGCATC CCATACGGCA ATTCCCAGAT GTTGAAAGTG 
GAGACAGTGG ATCCGTCACT GAAACCAGAA CAGCAGGCCA TTCAAGAGGT ATACGTCAAG 
CCGTATTCTA CTTTTAGAAG TCACGGTAAC TACTTGCACG TCTCAGTAGG AGCAAGTCGA 
GTGGTGGTCG GTGGCAGTAT CAGTGTCCAA GTGTACATCA AATCCTCTTT ACCAAACCAC 
AGAGCCCTCG TGGAGCATCT CACATACATT GTGTTAAATA AGGGGAAAAT AATTCAGGCT 
GGTAGAGTGA ATGTCAAAGG CCAGGACGTA ACAAGCGTTC ATCTGCTCAT TACCTCAGAA 
ATGCTACCTG CATTTCGGTT TGTGGCGTAT TACGTTTTGC CATGGCAGCA TAGGGCTGAA 
GTGGTGGCAG ACTCAGTGTT TGTTGATGTG GAGGACCGGT GTGTAGGTTC GCTTAATGTT 
GGACCAGTGG ATGGAGAAAT AGTCAGTTCT TACTCACCAG GCAGCAGCTT TAAATTTCAA 
GTGAAAGGTG ATGTTGGAGC AAAGGTCAGC CTGGTAGCTG TGGATAATGC CATCTTCCTG 
TTGAGTAAAA ACCGCCTGAC GCAGAAAAAG GTGTGGGAAG TGGTGGATCA GGCTGATATG 
GGCTGCACCG CAGGAGGAGG CAAGGATAAT ATGGGTGTTT TCAGTGATGC AGGACTGATG 
TTTCACTCCA GCACAGGAGG TTCAACTGAC TCCAAAGAAG ACTGCTCCAG TAAAACTAAA 
CGGCGAAGAC GTTCTGCAGC CAAACTGCAG CTCAGAGATC AGCTCGAGAA GGCCTATTCA 
GATGAGCTCC TGCAGAGGTG TTGTGTGGAC GGCATGAAGG AGATTCCCAT GCCTTATTCA 
TGCTACCGTC GTTCCCTCTA CATCACCGAG GACTGGAGCT GTGTGTTGGC TTTCCTGCGC 
TGCTGTGCAG AATACAGAGG AGAGGAGCTC GGCGTCATTA CCAGACCTCC AACAACCACT 
GCTCCACCAA CCACTGCTCC GCCAACCACT TCTCTACCCA CCCTCTTTGA CTTACCATAT 
TTAGAATATG ATTATATGGA AGAAAAGGTT ATATTGAGAG CGAGAATGAT AGAACCCATG 
CCAAAGCTGT ATGCCAGAGC TAATGATGTC GGAGTTCGTC AGGACAGTTA TCGTGAAGTC 
GTTGTAGAAG AAGAGGAAGA AGAGGCGGCT TCAGTGTTTG AGGAAGATGA TTTGTCTGAT 
CTAGAAGATA TTTATGTGCG CAGCAAGTTT TTTGAGTCGT GGTTGTGGAC CGATATCAGA 
CTGCCTAGCA CTGCCAAGTC TGATGGGTTG GCTGTGTTTC CAGTGGACAC CGTTCTTCCA 
GACAGTATCA CACAGTGGGG GTTTCTAGCT GTCAGTGCCT CACCTGAGAA AGGTGTCTGC 
GTTGCTGAAC CGTATAACGT CCGCGTGTGG AAGCCGTTCT TCATCGACCT GCGTTTGCCT 
CACTCAGTGT CCAGAAATGA ACAAGTGGAA ATCAAAGCTG TAATCCACAA CTACAAGAAC 
TCAACACTGG AGGTGATGGT GATCCTGGAG AAGACAGAGG ACATGTGTAG TGTGGCCTAT 
ACGGGACAGC ACAGACAGCA GGTTACAGTA GCAGCCGGTT CCTCTAAACT CATTACATAC 
ACCATCATCC CTCTCAAAAC AGGAGAGCTT CCTCTACAGG TCACTGCTGT CGCTGCCTCA 
TTCATGGGTC AAGATGCAGT GAAGAAGAAT CTACGCGTAG TAGTTGAAGG CATTCAGACA 
CTTAAAGTGA GGAGCTTTGT GTTGAACCCA TCAGAGAAAG GAGGCAGTGC TGGGCAACAG 
TTGATTCAAG TCAAGAAGCT GCAGTTGGAT GCTGTTGTGC CGAAATCTGT ACCAGAAACA 
TTTGTCAATG TCAGAGGTGA TTTGCTGGCA GACAGTATTG ATAACTCCAT TAAGGAGGAC 
TCATTAGCAG CGTTGATCCG GATGCCCGGA GGCTGTGTGG AGCAGAACCT GGCCAGAATC 
ACTCTGCCCC TCATCGCAGC TCATTACCTG GACCGCAGTG CAGACTGGGA GATTGTGGGA 
ATCAGTCGCA GAGAAGAGGC CATCAAATAC ATACAGAAAG GTTATGAAAA TCAACTTAAC 
TACAAAAAAT CAGACGACTC GTACCCTCCC TACGCCAATG AAGGCACCAG CACATGGATC 
ACTGCGTATG TGGTGAAGGT TTTCTCCATG GCGCATTCGT TCATTACCGT CAATGAGAAG 
CATCTGTGTG GCCCTCTGCT CTATTTACTC AAGAATAAAC AGGGTTATGA TGGAACTTTT 
CAAGAGGACA ATCCTGTTTA TGACACCAGT ATGACGGGAG CTATGCGGGG ATCTGAATAC 
AAAGCATCTT TGACAGCATT TGTACTCATT GCTTTAGCAG AGGCACAAGA TAAAGTTACT 
TGCCAAGATC AAGAAGTCAA CATACGGGAT AAGTCTAGAC AGGCAGCAGG GTATCTGACA 
GAGCATTTCT CCCGGTTGAC CAGGCCATAT ACAGCAGCTA TAGCATGCTA CGCGCTAGCC 
GTATCGAACA ACGCCTGCGT GAAGAGCATG TTGCTCAAAT TTGCCTCACC GGATCATTCC 
CACTGGCCTG ATTCAAACAA TTATTTTTTC ACACTGGAAG CTACAGGCTA CGCACTGCTA 
GCGCTGATCA AAAGTGGCCA CATGGAAGAG GCAGAAGCTC CATTTCGATG GCTGAATGAA 
CACCGTGGCA TTGGTGGAGG TTACGGCTCT ACTCAGTCCA CCATGGTGGT TCTTCAGGCC 
CTGTCTGAGT ATCTAGTGAA GAGGCCTCCT CCGTCAGACC TCAATTTGTT GGTTCAGCTC 
AGCGTTCCTG GTCGGAGTGA TGTTCGCTGG ACCTTCAATC CCAATTTGGC TTATGTGGCC 
CGTTCTTCAC GAGTCCCTCT TGATCAGGAA TTCTCAGTGG CGGCTTCTGG GAACGGCAAG 
GGTGTTTTGG AGGTAGTGAC GGTCTACCAT CAGCTTCCTG ATGTGTATGA GAACAGCACA 
TGCAACGGCT TTCAGCTGGA CGTCTCTATC GCTGAGACTA ATGAAAAAAC CTCATCGGAT 
GTAGAAAAGT CCTTCAGACT CAATGTCAAT GTGAGGGCTC TGGAAGAACG GCAAGTGAGG 
ATGGTTATTC TAGACATCGG CCTGCCTACA GGCTTTGAAC CTGAAAACTC TGATTTAGAG 
TTGCTCACAA ATTCAGTGGA TCGCTACATC AACAACTTCC AAGTGGTGGA CAACCTGAGC 
GACAGAGGAT CCCTCATTAT TCACCTGTTC AAGGTGTCCA ACAAAGAGGC AGAAATCATC 
TCATTCAGAC TCAATCAGAG GTTTAAAGTG GGGCTCCTTC AGCCTTCCAC AGTCAAAGTT 
TACCAGTACT ACAACAAAGA TAAAAGCTGT AGCAGGTTCT ACTCTCCTCC TGAAGACAAA 
CAGCAGCTGG ATCAGATCTG TGAAGGCGAC GTGTGCCGCT GCTCGCAGGG AGACTGTTGT 
GTAATGAAGG AAGACTCCGC GTCTTTTGGT AAAGAGCAGA GGAATGCCGC TGTATGCAAC 
GGATTACATC ATGCCTACAG AGTCAAGCTG ATCAGCATCA GCCAGAGTCA CTATTACCAG 
TATGAGATGG AGATTGTGCA GGTTATTAAA GAAGGGACGG AGGAAGGTCT GAATGAGAAG 
GAGAGGAGGA TGTTCCTCTC TCATGCGAGC TGCAGGACCA GGCTTAATTT GAGAGAGGGT 
CAGGAGTATC TGGTCATTGG GCCGATCACT GATGTTTGGA ATGCAGGAAG CACAGTGTCC 
AACAAGTATG TGTATACGCT TGGGAAAGAA ACGTGGGTTG AGCGCTGGCC AGATGAGTCT 
GAATGTGGAT CAACACTGCA GAAGAAATGT GACGAGCTGA AGAGCTTTGC AAAGAAACTG 
ATTGAAGACG GCTGTCAGGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005174089.1
CDS	137..5380
Translation	MLLQLLLWATLLCQICDGKAFPQRHKRTVWGPNMRQIEPVKDPSPSFSLLAPAKIRPGTKQNILVEGHNLAKVVKVTVVVYDYPNSQTALSRGSVSLNSDNNYSVLQTLEINPNLLLPDGKKKYVKLVAQFSNIYRAEKVMAVSFRSGHIFIQTDKPIYNPGDKVRFRAFVSDLEFRSSERTISLEIQNPDGIAVHGIGRVKAIDGVFSDIFSLSTVVKEGRWKVVAKFDQGKENVFSREFEVKKYVLPAFNVTLTPKTSHLSLDAEKLEVEITARYLYGAQVKGVAYVLFGIEINGEKKRLTSMKQIDDLNGGSVSLTMEEIKRAYPDINSLLGSTVYVKASVLTSSGSDLVEAEKSGIKIVNSPYMLTIKDTPKYYKPGLPLAMTVIVSNHDGSPTPNIPVKVTFLKRPISVHSGTINIHINMPKKRIPYGNSQMLKVETVDPSLKPEQQAIQEVYVKPYSTFRSHGNYLHVSVGASRVVVGGSISVQVYIKSSLPNHRALVEHLTYIVLNKGKIIQAGRVNVKGQDVTSVHLLITSEMLPAFRFVAYYVLPWQHRAEVVADSVFVDVEDRCVGSLNVGPVDGEIVSSYSPGSSFKFQVKGDVGAKVSLVAVDNAIFLLSKNRLTQKKVWEVVDQADMGCTAGGGKDNMGVFSDAGLMFHSSTGGSTDSKEDCSSKTKRRRRSAAKLQLRDQLEKAYSDELLQRCCVDGMKEIPMPYSCYRRSLYITEDWSCVLAFLRCCAEYRGEELGVITRPPTTTAPPTTAPPTTSLPTLFDLPYLEYDYMEEKVILRARMIEPMPKLYARANDVGVRQDSYREVVVEEEEEEAASVFEEDDLSDLEDIYVRSKFFESWLWTDIRLPSTAKSDGLAVFPVDTVLPDSITQWGFLAVSASPEKGVCVAEPYNVRVWKPFFIDLRLPHSVSRNEQVEIKAVIHNYKNSTLEVMVILEKTEDMCSVAYTGQHRQQVTVAAGSSKLITYTIIPLKTGELPLQVTAVAASFMGQDAVKKNLRVVVEGIQTLKVRSFVLNPSEKGGSAGQQLIQVKKLQLDAVVPKSVPETFVNVRGDLLADSIDNSIKEDSLAALIRMPGGCVEQNLARITLPLIAAHYLDRSADWEIVGISRREEAIKYIQKGYENQLNYKKSDDSYPPYANEGTSTWITAYVVKVFSMAHSFITVNEKHLCGPLLYLLKNKQGYDGTFQEDNPVYDTSMTGAMRGSEYKASLTAFVLIALAEAQDKVTCQDQEVNIRDKSRQAAGYLTEHFSRLTRPYTAAIACYALAVSNNACVKSMLLKFASPDHSHWPDSNNYFFTLEATGYALLALIKSGHMEEAEAPFRWLNEHRGIGGGYGSTQSTMVVLQALSEYLVKRPPPSDLNLLVQLSVPGRSDVRWTFNPNLAYVARSSRVPLDQEFSVAASGNGKGVLEVVTVYHQLPDVYENSTCNGFQLDVSIAETNEKTSSDVEKSFRLNVNVRALEERQVRMVILDIGLPTGFEPENSDLELLTNSVDRYINNFQVVDNLSDRGSLIIHLFKVSNKEAEIISFRLNQRFKVGLLQPSTVKVYQYYNKDKSCSRFYSPPEDKQQLDQICEGDVCRCSQGDCCVMKEDSASFGKEQRNAAVCNGLHHAYRVKLISISQSHYYQYEMEIVQVIKEGTEEGLNEKERRMFLSHASCRTRLNLREGQEYLVIGPITDVWNAGSTVSNKYVYTLGKETWVERWPDESECGSTLQKKCDELKSFAKKLIEDGCQA

Target ORF information:

RefSeq Version	XM_005174032.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio complement component c3b, tandem duplicate 2 (c3b.2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005174032.2

ORF Insert Sequence:

ATGCTTCTTC AGCTGCTGTT ATGGGCGACC CTGCTCTGTC AAATCTGCGA TGGAAAGGCC 
TTCCCTCAAC GACACAAGAG GACGGTTTGG GGACCTAATA TGCGACAAAT TGAGCCGGTC 
AAAGACCCTT CTCCGTCGTT TTCTCTTCTA GCTCCAGCAA AAATAAGGCC TGGAACAAAA 
CAGAACATCC TGGTGGAGGG ACACAACCTG GCAAAGGTGG TAAAAGTCAC TGTTGTGGTT 
TACGACTATC CAAACTCCCA AACTGCCTTG TCACGGGGCT CAGTTTCCCT CAATTCAGAC 
AACAACTACA GCGTCCTGCA AACCCTAGAG ATCAACCCAA ATTTGCTACT TCCAGATGGA 
AAAAAGAAGT ATGTGAAGCT TGTTGCTCAG TTCAGCAACA TCTATCGTGC AGAAAAAGTA 
ATGGCGGTGT CATTTCGCTC TGGACACATC TTCATTCAGA CCGACAAACC TATTTACAAC 
CCTGGAGACA AAGTGCGTTT CAGGGCTTTT GTGTCTGATT TGGAGTTTCG TTCTTCTGAG 
AGAACCATAT CTCTGGAGAT TCAGAATCCA GATGGAATTG CGGTGCATGG TATTGGGAGA 
GTTAAAGCAA TCGATGGGGT CTTTTCTGAC ATCTTCTCAC TGTCTACAGT AGTCAAGGAG 
GGCAGATGGA AAGTTGTAGC AAAGTTTGAT CAGGGAAAAG AGAATGTTTT CAGCAGAGAG 
TTTGAAGTGA AGAAATATGT GCTTCCAGCA TTCAACGTCA CACTTACTCC TAAAACATCC 
CACCTCAGTC TGGATGCAGA AAAGTTGGAA GTTGAAATAA CAGCCAGGTA TCTTTATGGC 
GCGCAAGTGA AGGGTGTTGC GTATGTGCTG TTTGGTATTG AAATTAATGG TGAAAAGAAG 
AGACTCACAT CTATGAAGCA AATTGATGAT CTAAATGGAG GAAGTGTTAG TTTGACTATG 
GAGGAGATAA AGAGAGCTTA TCCTGACATA AACAGCCTCC TGGGGTCTAC GGTGTACGTC 
AAAGCATCAG TATTGACTAG TTCAGGTAGC GATCTTGTGG AAGCAGAGAA ATCCGGCATT 
AAAATCGTAA ATTCGCCCTA CATGCTTACC ATCAAGGACA CACCAAAGTA TTATAAACCT 
GGTCTGCCGT TAGCCATGAC GGTTATCGTG AGTAACCATG ATGGATCTCC AACTCCTAAT 
ATTCCTGTAA AAGTCACCTT TCTTAAAAGG CCAATCAGTG TCCACAGTGG CACCATCAAC 
ATTCACATCA ACATGCCAAA AAAGCGCATC CCATACGGCA ATTCCCAGAT GTTGAAAGTG 
GAGACAGTGG ATCCGTCACT GAAACCAGAA CAGCAGGCCA TTCAAGAGGT ATACGTCAAG 
CCGTATTCTA CTTTTAGAAG TCACGGTAAC TACTTGCACG TCTCAGTAGG AGCAAGTCGA 
GTGGTGGTCG GTGGCAGTAT CAGTGTCCAA GTGTACATCA AATCCTCTTT ACCAAACCAC 
AGAGCCCTCG TGGAGCATCT CACATACATT GTGTTAAATA AGGGGAAAAT AATTCAGGCT 
GGTAGAGTGA ATGTCAAAGG CCAGGACGTA ACAAGCGTTC ATCTGCTCAT TACCTCAGAA 
ATGCTACCTG CATTTCGGTT TGTGGCGTAT TACGTTTTGC CATGGCAGCA TAGGGCTGAA 
GTGGTGGCAG ACTCAGTGTT TGTTGATGTG GAGGACCGGT GTGTAGGTTC GCTTAATGTT 
GGACCAGTGG ATGGAGAAAT AGTCAGTTCT TACTCACCAG GCAGCAGCTT TAAATTTCAA 
GTGAAAGGTG ATGTTGGAGC AAAGGTCAGC CTGGTAGCTG TGGATAATGC CATCTTCCTG 
TTGAGTAAAA ACCGCCTGAC GCAGAAAAAG GTGTGGGAAG TGGTGGATCA GGCTGATATG 
GGCTGCACCG CAGGAGGAGG CAAGGATAAT ATGGGTGTTT TCAGTGATGC AGGACTGATG 
TTTCACTCCA GCACAGGAGG TTCAACTGAC TCCAAAGAAG ACTGCTCCAG TAAAACTAAA 
CGGCGAAGAC GTTCTGCAGC CAAACTGCAG CTCAGAGATC AGCTCGAGAA GGCCTATTCA 
GATGAGCTCC TGCAGAGGTG TTGTGTGGAC GGCATGAAGG AGATTCCCAT GCCTTATTCA 
TGCTACCGTC GTTCCCTCTA CATCACCGAG GACTGGAGCT GTGTGTTGGC TTTCCTGCGC 
TGCTGTGCAG AATACAGAGG AGAGGAGCTC GGCGTCATTA CCAGACCTCC AACAACCACT 
GCTCCACCAA CCACTGCTCC GCCAACCACT TCTCTACCCA CCCTCTTTGA CTTACCATAT 
TTAGAATATG ATTATATGGA AGAAAAGGTT ATATTGAGAG CGAGAATGAT AGAACCCATG 
CCAAAGCTGT ATGCCAGAGC TAATGATGTC GGAGTTCGTC AGGACAGTTA TCGTGAAGTC 
GTTGTAGAAG AAGAGGAAGA AGAGGCGGCT TCAGTGTTTG AGGAAGATGA TTTGTCTGAT 
CTAGAAGATA TTTATGTGCG CAGCAAGTTT TTTGAGTCGT GGTTGTGGAC CGATATCAGA 
CTGCCTAGCA CTGCCAAGTC TGATGGGTTG GCTGTGTTTC CAGTGGACAC CGTTCTTCCA 
GACAGTATCA CACAGTGGGG GTTTCTAGCT GTCAGTGCCT CACCTGAGAA AGGTGTCTGC 
GTTGCTGAAC CGTATAACGT CCGCGTGTGG AAGCCGTTCT TCATCGACCT GCGTTTGCCT 
CACTCAGTGT CCAGAAATGA ACAAGTGGAA ATCAAAGCTG TAATCCACAA CTACAAGAAC 
TCAACACTGG AGGTGATGGT GATCCTGGAG AAGACAGAGG ACATGTGTAG TGTGGCCTAT 
ACGGGACAGC ACAGACAGCA GGTTACAGTA GCAGCCGGTT CCTCTAAACT CATTACATAC 
ACCATCATCC CTCTCAAAAC AGGAGAGCTT CCTCTACAGG TCACTGCTGT CGCTGCCTCA 
TTCATGGGTC AAGATGCAGT GAAGAAGAAT CTACGCGTAG TAGTTGAAGG CATTCAGACA 
CTTAAAGTGA GGAGCTTTGT GTTGAACCCA TCAGAGAAAG GAGGCAGTGC TGGGCAACAG 
TTGATTCAAG TCAAGAAGCT GCAGTTGGAT GCTGTTGTGC CGAAATCTGT ACCAGAAACA 
TTTGTCAATG TCAGAGGTGA TTTGCTGGCA GACAGTATTG ATAACTCCAT TAAGGAGGAC 
TCATTAGCAG CGTTGATCCG GATGCCCGGA GGCTGTGTGG AGCAGAACCT GGCCAGAATC 
ACTCTGCCCC TCATCGCAGC TCATTACCTG GACCGCAGTG CAGACTGGGA GATTGTGGGA 
ATCAGTCGCA GAGAAGAGGC CATCAAATAC ATACAGAAAG GTTATGAAAA TCAACTTAAC 
TACAAAAAAT CAGACGACTC GTACCCTCCC TACGCCAATG AAGGCACCAG CACATGGATC 
ACTGCGTATG TGGTGAAGGT TTTCTCCATG GCGCATTCGT TCATTACCGT CAATGAGAAG 
CATCTGTGTG GCCCTCTGCT CTATTTACTC AAGAATAAAC AGGGTTATGA TGGAACTTTT 
CAAGAGGACA ATCCTGTTTA TGACACCAGT ATGACGGGAG CTATGCGGGG ATCTGAATAC 
AAAGCATCTT TGACAGCATT TGTACTCATT GCTTTAGCAG AGGCACAAGA TAAAGTTACT 
TGCCAAGATC AAGAAGTCAA CATACGGGAT AAGTCTAGAC AGGCAGCAGG GTATCTGACA 
GAGCATTTCT CCCGGTTGAC CAGGCCATAT ACAGCAGCTA TAGCATGCTA CGCGCTAGCC 
GTATCGAACA ACGCCTGCGT GAAGAGCATG TTGCTCAAAT TTGCCTCACC GGATCATTCC 
CACTGGCCTG ATTCAAACAA TTATTTTTTC ACACTGGAAG CTACAGGCTA CGCACTGCTA 
GCGCTGATCA AAAGTGGCCA CATGGAAGAG GCAGAAGCTC CATTTCGATG GCTGAATGAA 
CACCGTGGCA TTGGTGGAGG TTACGGCTCT ACTCAGTCCA CCATGGTGGT TCTTCAGGCC 
CTGTCTGAGT ATCTAGTGAA GAGGCCTCCT CCGTCAGACC TCAATTTGTT GGTTCAGCTC 
AGCGTTCCTG GTCGGAGTGA TGTTCGCTGG ACCTTCAATC CCAATTTGGC TTATGTGGCC 
CGTTCTTCAC GAGTCCCTCT TGATCAGGAA TTCTCAGTGG CGGCTTCTGG GAACGGCAAG 
GGTGTTTTGG AGGTAGTGAC GGTCTACCAT CAGCTTCCTG ATGTGTATGA GAACAGCACA 
TGCAACGGCT TTCAGCTGGA CGTCTCTATC GCTGAGACTA ATGAAAAAAC CTCATCGGAT 
GTAGAAAAGT CCTTCAGACT CAATGTCAAT GTGAGGGCTC TGGAAGAACG GCAAGTGAGG 
ATGGTTATTC TAGACATCGG CCTGCCTACA GGCTTTGAAC CTGAAAACTC TGATTTAGAG 
TTGCTCACAA ATTCAGTGGA TCGCTACATC AACAACTTCC AAGTGGTGGA CAACCTGAGC 
GACAGAGGAT CCCTCATTAT TCACCTGTTC AAGGTGTCCA ACAAAGAGGC AGAAATCATC 
TCATTCAGAC TCAATCAGAG GTTTAAAGTG GGGCTCCTTC AGCCTTCCAC AGTCAAAGTT 
TACCAGTACT ACAACAAAGA TAAAAGCTGT AGCAGGTTCT ACTCTCCTCC TGAAGACAAA 
CAGCAGCTGG ATCAGATCTG TGAAGGCGAC GTGTGCCGCT GCTCGCAGGG AGACTGTTGT 
GTAATGAAGG AAGACTCCGC GTCTTTTGGT AAAGAGCAGA GGAATGCCGC TGTATGCAAC 
GGATTACATC ATGCCTACAG AGTCAAGCTG ATCAGCATCA GCCAGAGTCA CTATTACCAG 
TATGAGATGG AGATTGTGCA GGTTATTAAA GAAGGGACGG AGGAAGGTCT GAATGAGAAG 
GAGAGGAGGA TGTTCCTCTC TCATGCGAGC TGCAGGACCA GGCTTAATTT GAGAGAGGGT 
CAGGAGTATC TGGTCATTGG GCCGATCACT GATGTTTGGA ATGCAGGAAG CACAGTGTCC 
AACAAGTATG TGTATACGCT TGGGAAAGAA ACGTGGGTTG AGCGCTGGCC AGATGAGTCT 
GAATGTGGAT CAACACTGCA GAAGAAATGT GACGAGCTGA AGAGCTTTGC AAAGAAACTG 
ATTGAAGACG GCTGTCAGGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

c3b.2 ( XM_005174032.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005174089.1 Danio rerio complement component c3b, tandem duplicate 2 (c3b.2), transcript variant X2, mRNA.

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CloneID	ODa27026
Clone ID Related Accession (Same CDS sequence)	XM_005174032.2 , XM_005174032.3
Accession Version	XM_005174032.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	complement component c3b, tandem duplicate 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005174032.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCTTC AGCTGCTGTT ATGGGCGACC CTGCTCTGTC AAATCTGCGA TGGAAAGGCC 
TTCCCTCAAC GACACAAGAG GACGGTTTGG GGACCTAATA TGCGACAAAT TGAGCCGGTC 
AAAGACCCTT CTCCGTCGTT TTCTCTTCTA GCTCCAGCAA AAATAAGGCC TGGAACAAAA 
CAGAACATCC TGGTGGAGGG ACACAACCTG GCAAAGGTGG TAAAAGTCAC TGTTGTGGTT 
TACGACTATC CAAACTCCCA AACTGCCTTG TCACGGGGCT CAGTTTCCCT CAATTCAGAC 
AACAACTACA GCGTCCTGCA AACCCTAGAG ATCAACCCAA ATTTGCTACT TCCAGATGGA 
AAAAAGAAGT ATGTGAAGCT TGTTGCTCAG TTCAGCAACA TCTATCGTGC AGAAAAAGTA 
ATGGCGGTGT CATTTCGCTC TGGACACATC TTCATTCAGA CCGACAAACC TATTTACAAC 
CCTGGAGACA AAGTGCGTTT CAGGGCTTTT GTGTCTGATT TGGAGTTTCG TTCTTCTGAG 
AGAACCATAT CTCTGGAGAT TCAGAATCCA GATGGAATTG CGGTGCATGG TATTGGGAGA 
GTTAAAGCAA TCGATGGGGT CTTTTCTGAC ATCTTCTCAC TGTCTACAGT AGTCAAGGAG 
GGCAGATGGA AAGTTGTAGC AAAGTTTGAT CAGGGAAAAG AGAATGTTTT CAGCAGAGAG 
TTTGAAGTGA AGAAATATGT GCTTCCAGCA TTCAACGTCA CACTTACTCC TAAAACATCC 
CACCTCAGTC TGGATGCAGA AAAGTTGGAA GTTGAAATAA CAGCCAGGTA TCTTTATGGC 
GCGCAAGTGA AGGGTGTTGC GTATGTGCTG TTTGGTATTG AAATTAATGG TGAAAAGAAG 
AGACTCACAT CTATGAAGCA AATTGATGAT CTAAATGGAG GAAGTGTTAG TTTGACTATG 
GAGGAGATAA AGAGAGCTTA TCCTGACATA AACAGCCTCC TGGGGTCTAC GGTGTACGTC 
AAAGCATCAG TATTGACTAG TTCAGGTAGC GATCTTGTGG AAGCAGAGAA ATCCGGCATT 
AAAATCGTAA ATTCGCCCTA CATGCTTACC ATCAAGGACA CACCAAAGTA TTATAAACCT 
GGTCTGCCGT TAGCCATGAC GGTTATCGTG AGTAACCATG ATGGATCTCC AACTCCTAAT 
ATTCCTGTAA AAGTCACCTT TCTTAAAAGG CCAATCAGTG TCCACAGTGG CACCATCAAC 
ATTCACATCA ACATGCCAAA AAAGCGCATC CCATACGGCA ATTCCCAGAT GTTGAAAGTG 
GAGACAGTGG ATCCGTCACT GAAACCAGAA CAGCAGGCCA TTCAAGAGGT ATACGTCAAG 
CCGTATTCTA CTTTTAGAAG TCACGGTAAC TACTTGCACG TCTCAGTAGG AGCAAGTCGA 
GTGGTGGTCG GTGGCAGTAT CAGTGTCCAA GTGTACATCA AATCCTCTTT ACCAAACCAC 
AGAGCCCTCG TGGAGCATCT CACATACATT GTGTTAAATA AGGGGAAAAT AATTCAGGCT 
GGTAGAGTGA ATGTCAAAGG CCAGGACGTA ACAAGCGTTC ATCTGCTCAT TACCTCAGAA 
ATGCTACCTG CATTTCGGTT TGTGGCGTAT TACGTTTTGC CATGGCAGCA TAGGGCTGAA 
GTGGTGGCAG ACTCAGTGTT TGTTGATGTG GAGGACCGGT GTGTAGGTTC GCTTAATGTT 
GGACCAGTGG ATGGAGAAAT AGTCAGTTCT TACTCACCAG GCAGCAGCTT TAAATTTCAA 
GTGAAAGGTG ATGTTGGAGC AAAGGTCAGC CTGGTAGCTG TGGATAATGC CATCTTCCTG 
TTGAGTAAAA ACCGCCTGAC GCAGAAAAAG GTGTGGGAAG TGGTGGATCA GGCTGATATG 
GGCTGCACCG CAGGAGGAGG CAAGGATAAT ATGGGTGTTT TCAGTGATGC AGGACTGATG 
TTTCACTCCA GCACAGGAGG TTCAACTGAC TCCAAAGAAG ACTGCTCCAG TAAAACTAAA 
CGGCGAAGAC GTTCTGCAGC CAAACTGCAG CTCAGAGATC AGCTCGAGAA GGCCTATTCA 
GATGAGCTCC TGCAGAGGTG TTGTGTGGAC GGCATGAAGG AGATTCCCAT GCCTTATTCA 
TGCTACCGTC GTTCCCTCTA CATCACCGAG GACTGGAGCT GTGTGTTGGC TTTCCTGCGC 
TGCTGTGCAG AATACAGAGG AGAGGAGCTC GGCGTCATTA CCAGACCTCC AACAACCACT 
GCTCCACCAA CCACTGCTCC GCCAACCACT TCTCTACCCA CCCTCTTTGA CTTACCATAT 
TTAGAATATG ATTATATGGA AGAAAAGGTT ATATTGAGAG CGAGAATGAT AGAACCCATG 
CCAAAGCTGT ATGCCAGAGC TAATGATGTC GGAGTTCGTC AGGACAGTTA TCGTGAAGTC 
GTTGTAGAAG AAGAGGAAGA AGAGGCGGCT TCAGTGTTTG AGGAAGATGA TTTGTCTGAT 
CTAGAAGATA TTTATGTGCG CAGCAAGTTT TTTGAGTCGT GGTTGTGGAC CGATATCAGA 
CTGCCTAGCA CTGCCAAGTC TGATGGGTTG GCTGTGTTTC CAGTGGACAC CGTTCTTCCA 
GACAGTATCA CACAGTGGGG GTTTCTAGCT GTCAGTGCCT CACCTGAGAA AGGTGTCTGC 
GTTGCTGAAC CGTATAACGT CCGCGTGTGG AAGCCGTTCT TCATCGACCT GCGTTTGCCT 
CACTCAGTGT CCAGAAATGA ACAAGTGGAA ATCAAAGCTG TAATCCACAA CTACAAGAAC 
TCAACACTGG AGGTGATGGT GATCCTGGAG AAGACAGAGG ACATGTGTAG TGTGGCCTAT 
ACGGGACAGC ACAGACAGCA GGTTACAGTA GCAGCCGGTT CCTCTAAACT CATTACATAC 
ACCATCATCC CTCTCAAAAC AGGAGAGCTT CCTCTACAGG TCACTGCTGT CGCTGCCTCA 
TTCATGGGTC AAGATGCAGT GAAGAAGAAT CTACGCGTAG TAGTTGAAGG CATTCAGACA 
CTTAAAGTGA GGAGCTTTGT GTTGAACCCA TCAGAGAAAG GAGGCAGTGC TGGGCAACAG 
TTGATTCAAG TCAAGAAGCT GCAGTTGGAT GCTGTTGTGC CGAAATCTGT ACCAGAAACA 
TTTGTCAATG TCAGAGGTGA TTTGCTGGCA GACAGTATTG ATAACTCCAT TAAGGAGGAC 
TCATTAGCAG CGTTGATCCG GATGCCCGGA GGCTGTGTGG AGCAGAACCT GGCCAGAATC 
ACTCTGCCCC TCATCGCAGC TCATTACCTG GACCGCAGTG CAGACTGGGA GATTGTGGGA 
ATCAGTCGCA GAGAAGAGGC CATCAAATAC ATACAGAAAG GTTATGAAAA TCAACTTAAC 
TACAAAAAAT CAGACGACTC GTACCCTCCC TACGCCAATG AAGGCACCAG CACATGGATC 
ACTGCGTATG TGGTGAAGGT TTTCTCCATG GCGCATTCGT TCATTACCGT CAATGAGAAG 
CATCTGTGTG GCCCTCTGCT CTATTTACTC AAGAATAAAC AGGGTTATGA TGGAACTTTT 
CAAGAGGACA ATCCTGTTTA TGACACCAGT ATGACGGGAG CTATGCGGGG ATCTGAATAC 
AAAGCATCTT TGACAGCATT TGTACTCATT GCTTTAGCAG AGGCACAAGA TAAAGTTACT 
TGCCAAGATC AAGAAGTCAA CATACGGGAT AAGTCTAGAC AGGCAGCAGG GTATCTGACA 
GAGCATTTCT CCCGGTTGAC CAGGCCATAT ACAGCAGCTA TAGCATGCTA CGCGCTAGCC 
GTATCGAACA ACGCCTGCGT GAAGAGCATG TTGCTCAAAT TTGCCTCACC GGATCATTCC 
CACTGGCCTG ATTCAAACAA TTATTTTTTC ACACTGGAAG CTACAGGCTA CGCACTGCTA 
GCGCTGATCA AAAGTGGCCA CATGGAAGAG GCAGAAGCTC CATTTCGATG GCTGAATGAA 
CACCGTGGCA TTGGTGGAGG TTACGGCTCT ACTCAGTCCA CCATGGTGGT TCTTCAGGCC 
CTGTCTGAGT ATCTAGTGAA GAGGCCTCCT CCGTCAGACC TCAATTTGTT GGTTCAGCTC 
AGCGTTCCTG GTCGGAGTGA TGTTCGCTGG ACCTTCAATC CCAATTTGGC TTATGTGGCC 
CGTTCTTCAC GAGTCCCTCT TGATCAGGAA TTCTCAGTGG CGGCTTCTGG GAACGGCAAG 
GGTGTTTTGG AGGTAGTGAC GGTCTACCAT CAGCTTCCTG ATGTGTATGA GAACAGCACA 
TGCAACGGCT TTCAGCTGGA CGTCTCTATC GCTGAGACTA ATGAAAAAAC CTCATCGGAT 
GTAGAAAAGT CCTTCAGACT CAATGTCAAT GTGAGGGCTC TGGAAGAACG GCAAGTGAGG 
ATGGTTATTC TAGACATCGG CCTGCCTACA GGCTTTGAAC CTGAAAACTC TGATTTAGAG 
TTGCTCACAA ATTCAGTGGA TCGCTACATC AACAACTTCC AAGTGGTGGA CAACCTGAGC 
GACAGAGGAT CCCTCATTAT TCACCTGTTC AAGGTGTCCA ACAAAGAGGC AGAAATCATC 
TCATTCAGAC TCAATCAGAG GTTTAAAGTG GGGCTCCTTC AGCCTTCCAC AGTCAAAGTT 
TACCAGTACT ACAACAAAGA TAAAAGCTGT AGCAGGTTCT ACTCTCCTCC TGAAGACAAA 
CAGCAGCTGG ATCAGATCTG TGAAGGCGAC GTGTGCCGCT GCTCGCAGGG AGACTGTTGT 
GTAATGAAGG AAGACTCCGC GTCTTTTGGT AAAGAGCAGA GGAATGCCGC TGTATGCAAC 
GGATTACATC ATGCCTACAG AGTCAAGCTG ATCAGCATCA GCCAGAGTCA CTATTACCAG 
TATGAGATGG AGATTGTGCA GGTTATTAAA GAAGGGACGG AGGAAGGTCT GAATGAGAAG 
GAGAGGAGGA TGTTCCTCTC TCATGCGAGC TGCAGGACCA GGCTTAATTT GAGAGAGGGT 
CAGGAGTATC TGGTCATTGG GCCGATCACT GATGTTTGGA ATGCAGGAAG CACAGTGTCC 
AACAAGTATG TGTATACGCT TGGGAAAGAA ACGTGGGTTG AGCGCTGGCC AGATGAGTCT 
GAATGTGGAT CAACACTGCA GAAGAAATGT GACGAGCTGA AGAGCTTTGC AAAGAAACTG 
ATTGAAGACG GCTGTCAGGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005174089.1
CDS	170..5413
Translation	MLLQLLLWATLLCQICDGKAFPQRHKRTVWGPNMRQIEPVKDPSPSFSLLAPAKIRPGTKQNILVEGHNLAKVVKVTVVVYDYPNSQTALSRGSVSLNSDNNYSVLQTLEINPNLLLPDGKKKYVKLVAQFSNIYRAEKVMAVSFRSGHIFIQTDKPIYNPGDKVRFRAFVSDLEFRSSERTISLEIQNPDGIAVHGIGRVKAIDGVFSDIFSLSTVVKEGRWKVVAKFDQGKENVFSREFEVKKYVLPAFNVTLTPKTSHLSLDAEKLEVEITARYLYGAQVKGVAYVLFGIEINGEKKRLTSMKQIDDLNGGSVSLTMEEIKRAYPDINSLLGSTVYVKASVLTSSGSDLVEAEKSGIKIVNSPYMLTIKDTPKYYKPGLPLAMTVIVSNHDGSPTPNIPVKVTFLKRPISVHSGTINIHINMPKKRIPYGNSQMLKVETVDPSLKPEQQAIQEVYVKPYSTFRSHGNYLHVSVGASRVVVGGSISVQVYIKSSLPNHRALVEHLTYIVLNKGKIIQAGRVNVKGQDVTSVHLLITSEMLPAFRFVAYYVLPWQHRAEVVADSVFVDVEDRCVGSLNVGPVDGEIVSSYSPGSSFKFQVKGDVGAKVSLVAVDNAIFLLSKNRLTQKKVWEVVDQADMGCTAGGGKDNMGVFSDAGLMFHSSTGGSTDSKEDCSSKTKRRRRSAAKLQLRDQLEKAYSDELLQRCCVDGMKEIPMPYSCYRRSLYITEDWSCVLAFLRCCAEYRGEELGVITRPPTTTAPPTTAPPTTSLPTLFDLPYLEYDYMEEKVILRARMIEPMPKLYARANDVGVRQDSYREVVVEEEEEEAASVFEEDDLSDLEDIYVRSKFFESWLWTDIRLPSTAKSDGLAVFPVDTVLPDSITQWGFLAVSASPEKGVCVAEPYNVRVWKPFFIDLRLPHSVSRNEQVEIKAVIHNYKNSTLEVMVILEKTEDMCSVAYTGQHRQQVTVAAGSSKLITYTIIPLKTGELPLQVTAVAASFMGQDAVKKNLRVVVEGIQTLKVRSFVLNPSEKGGSAGQQLIQVKKLQLDAVVPKSVPETFVNVRGDLLADSIDNSIKEDSLAALIRMPGGCVEQNLARITLPLIAAHYLDRSADWEIVGISRREEAIKYIQKGYENQLNYKKSDDSYPPYANEGTSTWITAYVVKVFSMAHSFITVNEKHLCGPLLYLLKNKQGYDGTFQEDNPVYDTSMTGAMRGSEYKASLTAFVLIALAEAQDKVTCQDQEVNIRDKSRQAAGYLTEHFSRLTRPYTAAIACYALAVSNNACVKSMLLKFASPDHSHWPDSNNYFFTLEATGYALLALIKSGHMEEAEAPFRWLNEHRGIGGGYGSTQSTMVVLQALSEYLVKRPPPSDLNLLVQLSVPGRSDVRWTFNPNLAYVARSSRVPLDQEFSVAASGNGKGVLEVVTVYHQLPDVYENSTCNGFQLDVSIAETNEKTSSDVEKSFRLNVNVRALEERQVRMVILDIGLPTGFEPENSDLELLTNSVDRYINNFQVVDNLSDRGSLIIHLFKVSNKEAEIISFRLNQRFKVGLLQPSTVKVYQYYNKDKSCSRFYSPPEDKQQLDQICEGDVCRCSQGDCCVMKEDSASFGKEQRNAAVCNGLHHAYRVKLISISQSHYYQYEMEIVQVIKEGTEEGLNEKERRMFLSHASCRTRLNLREGQEYLVIGPITDVWNAGSTVSNKYVYTLGKETWVERWPDESECGSTLQKKCDELKSFAKKLIEDGCQA

Target ORF information:

RefSeq Version	XM_005174032.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio complement component c3b, tandem duplicate 2 (c3b.2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005174032.3

ORF Insert Sequence:

ATGCTTCTTC AGCTGCTGTT ATGGGCGACC CTGCTCTGTC AAATCTGCGA TGGAAAGGCC 
TTCCCTCAAC GACACAAGAG GACGGTTTGG GGACCTAATA TGCGACAAAT TGAGCCGGTC 
AAAGACCCTT CTCCGTCGTT TTCTCTTCTA GCTCCAGCAA AAATAAGGCC TGGAACAAAA 
CAGAACATCC TGGTGGAGGG ACACAACCTG GCAAAGGTGG TAAAAGTCAC TGTTGTGGTT 
TACGACTATC CAAACTCCCA AACTGCCTTG TCACGGGGCT CAGTTTCCCT CAATTCAGAC 
AACAACTACA GCGTCCTGCA AACCCTAGAG ATCAACCCAA ATTTGCTACT TCCAGATGGA 
AAAAAGAAGT ATGTGAAGCT TGTTGCTCAG TTCAGCAACA TCTATCGTGC AGAAAAAGTA 
ATGGCGGTGT CATTTCGCTC TGGACACATC TTCATTCAGA CCGACAAACC TATTTACAAC 
CCTGGAGACA AAGTGCGTTT CAGGGCTTTT GTGTCTGATT TGGAGTTTCG TTCTTCTGAG 
AGAACCATAT CTCTGGAGAT TCAGAATCCA GATGGAATTG CGGTGCATGG TATTGGGAGA 
GTTAAAGCAA TCGATGGGGT CTTTTCTGAC ATCTTCTCAC TGTCTACAGT AGTCAAGGAG 
GGCAGATGGA AAGTTGTAGC AAAGTTTGAT CAGGGAAAAG AGAATGTTTT CAGCAGAGAG 
TTTGAAGTGA AGAAATATGT GCTTCCAGCA TTCAACGTCA CACTTACTCC TAAAACATCC 
CACCTCAGTC TGGATGCAGA AAAGTTGGAA GTTGAAATAA CAGCCAGGTA TCTTTATGGC 
GCGCAAGTGA AGGGTGTTGC GTATGTGCTG TTTGGTATTG AAATTAATGG TGAAAAGAAG 
AGACTCACAT CTATGAAGCA AATTGATGAT CTAAATGGAG GAAGTGTTAG TTTGACTATG 
GAGGAGATAA AGAGAGCTTA TCCTGACATA AACAGCCTCC TGGGGTCTAC GGTGTACGTC 
AAAGCATCAG TATTGACTAG TTCAGGTAGC GATCTTGTGG AAGCAGAGAA ATCCGGCATT 
AAAATCGTAA ATTCGCCCTA CATGCTTACC ATCAAGGACA CACCAAAGTA TTATAAACCT 
GGTCTGCCGT TAGCCATGAC GGTTATCGTG AGTAACCATG ATGGATCTCC AACTCCTAAT 
ATTCCTGTAA AAGTCACCTT TCTTAAAAGG CCAATCAGTG TCCACAGTGG CACCATCAAC 
ATTCACATCA ACATGCCAAA AAAGCGCATC CCATACGGCA ATTCCCAGAT GTTGAAAGTG 
GAGACAGTGG ATCCGTCACT GAAACCAGAA CAGCAGGCCA TTCAAGAGGT ATACGTCAAG 
CCGTATTCTA CTTTTAGAAG TCACGGTAAC TACTTGCACG TCTCAGTAGG AGCAAGTCGA 
GTGGTGGTCG GTGGCAGTAT CAGTGTCCAA GTGTACATCA AATCCTCTTT ACCAAACCAC 
AGAGCCCTCG TGGAGCATCT CACATACATT GTGTTAAATA AGGGGAAAAT AATTCAGGCT 
GGTAGAGTGA ATGTCAAAGG CCAGGACGTA ACAAGCGTTC ATCTGCTCAT TACCTCAGAA 
ATGCTACCTG CATTTCGGTT TGTGGCGTAT TACGTTTTGC CATGGCAGCA TAGGGCTGAA 
GTGGTGGCAG ACTCAGTGTT TGTTGATGTG GAGGACCGGT GTGTAGGTTC GCTTAATGTT 
GGACCAGTGG ATGGAGAAAT AGTCAGTTCT TACTCACCAG GCAGCAGCTT TAAATTTCAA 
GTGAAAGGTG ATGTTGGAGC AAAGGTCAGC CTGGTAGCTG TGGATAATGC CATCTTCCTG 
TTGAGTAAAA ACCGCCTGAC GCAGAAAAAG GTGTGGGAAG TGGTGGATCA GGCTGATATG 
GGCTGCACCG CAGGAGGAGG CAAGGATAAT ATGGGTGTTT TCAGTGATGC AGGACTGATG 
TTTCACTCCA GCACAGGAGG TTCAACTGAC TCCAAAGAAG ACTGCTCCAG TAAAACTAAA 
CGGCGAAGAC GTTCTGCAGC CAAACTGCAG CTCAGAGATC AGCTCGAGAA GGCCTATTCA 
GATGAGCTCC TGCAGAGGTG TTGTGTGGAC GGCATGAAGG AGATTCCCAT GCCTTATTCA 
TGCTACCGTC GTTCCCTCTA CATCACCGAG GACTGGAGCT GTGTGTTGGC TTTCCTGCGC 
TGCTGTGCAG AATACAGAGG AGAGGAGCTC GGCGTCATTA CCAGACCTCC AACAACCACT 
GCTCCACCAA CCACTGCTCC GCCAACCACT TCTCTACCCA CCCTCTTTGA CTTACCATAT 
TTAGAATATG ATTATATGGA AGAAAAGGTT ATATTGAGAG CGAGAATGAT AGAACCCATG 
CCAAAGCTGT ATGCCAGAGC TAATGATGTC GGAGTTCGTC AGGACAGTTA TCGTGAAGTC 
GTTGTAGAAG AAGAGGAAGA AGAGGCGGCT TCAGTGTTTG AGGAAGATGA TTTGTCTGAT 
CTAGAAGATA TTTATGTGCG CAGCAAGTTT TTTGAGTCGT GGTTGTGGAC CGATATCAGA 
CTGCCTAGCA CTGCCAAGTC TGATGGGTTG GCTGTGTTTC CAGTGGACAC CGTTCTTCCA 
GACAGTATCA CACAGTGGGG GTTTCTAGCT GTCAGTGCCT CACCTGAGAA AGGTGTCTGC 
GTTGCTGAAC CGTATAACGT CCGCGTGTGG AAGCCGTTCT TCATCGACCT GCGTTTGCCT 
CACTCAGTGT CCAGAAATGA ACAAGTGGAA ATCAAAGCTG TAATCCACAA CTACAAGAAC 
TCAACACTGG AGGTGATGGT GATCCTGGAG AAGACAGAGG ACATGTGTAG TGTGGCCTAT 
ACGGGACAGC ACAGACAGCA GGTTACAGTA GCAGCCGGTT CCTCTAAACT CATTACATAC 
ACCATCATCC CTCTCAAAAC AGGAGAGCTT CCTCTACAGG TCACTGCTGT CGCTGCCTCA 
TTCATGGGTC AAGATGCAGT GAAGAAGAAT CTACGCGTAG TAGTTGAAGG CATTCAGACA 
CTTAAAGTGA GGAGCTTTGT GTTGAACCCA TCAGAGAAAG GAGGCAGTGC TGGGCAACAG 
TTGATTCAAG TCAAGAAGCT GCAGTTGGAT GCTGTTGTGC CGAAATCTGT ACCAGAAACA 
TTTGTCAATG TCAGAGGTGA TTTGCTGGCA GACAGTATTG ATAACTCCAT TAAGGAGGAC 
TCATTAGCAG CGTTGATCCG GATGCCCGGA GGCTGTGTGG AGCAGAACCT GGCCAGAATC 
ACTCTGCCCC TCATCGCAGC TCATTACCTG GACCGCAGTG CAGACTGGGA GATTGTGGGA 
ATCAGTCGCA GAGAAGAGGC CATCAAATAC ATACAGAAAG GTTATGAAAA TCAACTTAAC 
TACAAAAAAT CAGACGACTC GTACCCTCCC TACGCCAATG AAGGCACCAG CACATGGATC 
ACTGCGTATG TGGTGAAGGT TTTCTCCATG GCGCATTCGT TCATTACCGT CAATGAGAAG 
CATCTGTGTG GCCCTCTGCT CTATTTACTC AAGAATAAAC AGGGTTATGA TGGAACTTTT 
CAAGAGGACA ATCCTGTTTA TGACACCAGT ATGACGGGAG CTATGCGGGG ATCTGAATAC 
AAAGCATCTT TGACAGCATT TGTACTCATT GCTTTAGCAG AGGCACAAGA TAAAGTTACT 
TGCCAAGATC AAGAAGTCAA CATACGGGAT AAGTCTAGAC AGGCAGCAGG GTATCTGACA 
GAGCATTTCT CCCGGTTGAC CAGGCCATAT ACAGCAGCTA TAGCATGCTA CGCGCTAGCC 
GTATCGAACA ACGCCTGCGT GAAGAGCATG TTGCTCAAAT TTGCCTCACC GGATCATTCC 
CACTGGCCTG ATTCAAACAA TTATTTTTTC ACACTGGAAG CTACAGGCTA CGCACTGCTA 
GCGCTGATCA AAAGTGGCCA CATGGAAGAG GCAGAAGCTC CATTTCGATG GCTGAATGAA 
CACCGTGGCA TTGGTGGAGG TTACGGCTCT ACTCAGTCCA CCATGGTGGT TCTTCAGGCC 
CTGTCTGAGT ATCTAGTGAA GAGGCCTCCT CCGTCAGACC TCAATTTGTT GGTTCAGCTC 
AGCGTTCCTG GTCGGAGTGA TGTTCGCTGG ACCTTCAATC CCAATTTGGC TTATGTGGCC 
CGTTCTTCAC GAGTCCCTCT TGATCAGGAA TTCTCAGTGG CGGCTTCTGG GAACGGCAAG 
GGTGTTTTGG AGGTAGTGAC GGTCTACCAT CAGCTTCCTG ATGTGTATGA GAACAGCACA 
TGCAACGGCT TTCAGCTGGA CGTCTCTATC GCTGAGACTA ATGAAAAAAC CTCATCGGAT 
GTAGAAAAGT CCTTCAGACT CAATGTCAAT GTGAGGGCTC TGGAAGAACG GCAAGTGAGG 
ATGGTTATTC TAGACATCGG CCTGCCTACA GGCTTTGAAC CTGAAAACTC TGATTTAGAG 
TTGCTCACAA ATTCAGTGGA TCGCTACATC AACAACTTCC AAGTGGTGGA CAACCTGAGC 
GACAGAGGAT CCCTCATTAT TCACCTGTTC AAGGTGTCCA ACAAAGAGGC AGAAATCATC 
TCATTCAGAC TCAATCAGAG GTTTAAAGTG GGGCTCCTTC AGCCTTCCAC AGTCAAAGTT 
TACCAGTACT ACAACAAAGA TAAAAGCTGT AGCAGGTTCT ACTCTCCTCC TGAAGACAAA 
CAGCAGCTGG ATCAGATCTG TGAAGGCGAC GTGTGCCGCT GCTCGCAGGG AGACTGTTGT 
GTAATGAAGG AAGACTCCGC GTCTTTTGGT AAAGAGCAGA GGAATGCCGC TGTATGCAAC 
GGATTACATC ATGCCTACAG AGTCAAGCTG ATCAGCATCA GCCAGAGTCA CTATTACCAG 
TATGAGATGG AGATTGTGCA GGTTATTAAA GAAGGGACGG AGGAAGGTCT GAATGAGAAG 
GAGAGGAGGA TGTTCCTCTC TCATGCGAGC TGCAGGACCA GGCTTAATTT GAGAGAGGGT 
CAGGAGTATC TGGTCATTGG GCCGATCACT GATGTTTGGA ATGCAGGAAG CACAGTGTCC 
AACAAGTATG TGTATACGCT TGGGAAAGAA ACGTGGGTTG AGCGCTGGCC AGATGAGTCT 
GAATGTGGAT CAACACTGCA GAAGAAATGT GACGAGCTGA AGAGCTTTGC AAAGAAACTG 
ATTGAAGACG GCTGTCAGGC TTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

c3b.2 ( XM_009296391.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294666.1 Danio rerio complement component c3b, tandem duplicate 2 (c3b.2), transcript variant X1, mRNA.

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CloneID	ODa44848
Clone ID Related Accession (Same CDS sequence)	XM_009296391.3 , XM_009296391.2
Accession Version	XM_009296391.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	complement component c3b, tandem duplicate 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296391.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCTTC AGCTGCTGTT ATGGGCGACC CTGCTCTGTC AAATCTGCGA TGGAAAGGCC 
TTCCCTCAAC GACACAAGAG GACGGTTTGG GGACCTAATA TGCGACAAAT TGAGCCGGTC 
AAAGACCCTT CTCCGTCGTT TTCTCTTCTA GCTCCAGCAA AAATAAGGCC TGGAACAAAA 
CAGAACATCC TGGTGGAGGG ACACAACCTG GCAAAGGTGG TAAAAGTCAC TGTTGTGGTT 
TACGACTATC CAAACTCCCA AACTGCCTTG TCACGGGGCT CAGTTTCCCT CAATTCAGAC 
AACAACTACA GCGTCCTGCA AACCCTAGAG ATCAACCCAA ATTTGCTACT TCCAGATGGA 
AAAAAGAAGT ATGTGAAGCT TGTTGCTCAG TTCAGCAACA TCTATCGTGC AGAAAAAGTA 
ATGGCGGTGT CATTTCGCTC TGGACACATC TTCATTCAGA CCGACAAACC TATTTACAAC 
CCTGGAGACA AAGTGCGTTT CAGGGCTTTT GTGTCTGATT TGGAGTTTCG TTCTTCTGAG 
AGAACCATAT CTCTGGAGAT TCAGAATCCA GATGGAATTG CGGTGCATGG TATTGGGAGA 
GTTAAAGCAA TCGATGGGGT CTTTTCTGAC ATCTTCTCAC TGTCTACAGT AGTCAAGGAG 
GGCAGATGGA AAGTTGTAGC AAAGTTTGAT CAGGGAAAAG AGAATGTTTT CAGCAGAGAG 
TTTGAAGTGA AGAAATATGT GCTTCCAGCA TTCAACGTCA CACTTACTCC TAAAACATCC 
CACCTCAGTC TGGATGCAGA AAAGTTGGAA GTTGAAATAA CAGCCAGGTA TCTTTATGGC 
GCGCAAGTGA AGGGTGTTGC GTATGTGCTG TTTGGTATTG AAATTAATGG TGAAAAGAAG 
AGACTCACAT CTATGAAGCA AATTGATGAT CTAAATGGAG GAAGTGTTAG TTTGACTATG 
GAGGAGATAA AGAGAGCTTA TCCTGACATA AACAGCCTCC TGGGGTCTAC GGTGTACGTC 
AAAGCATCAG TATTGACTAG TTCAGGTAGC GATCTTGTGG AAGCAGAGAA ATCCGGCATT 
AAAATCGTAA ATTCGCCCTA CATGCTTACC ATCAAGGACA CACCAAAGTA TTATAAACCT 
GGTCTGCCGT TAGCCATGAC GGTTATCGTG AGTAACCATG ATGGATCTCC AACTCCTAAT 
ATTCCTGTAA AAGTCACCTT TCTTAAAAGG CCAATCAGTG TCCACAGTGG CACCATCAAC 
ATTCACATCA ACATGCCAAA AAAGCGCATC CCATACGGCA ATTCCCAGAT GTTGAAAGTG 
GAGACAGTGG ATCCGTCACT GAAACCAGAA CAGCAGGCCA TTCAAGAGGT ATACGTCAAG 
CCGTATTCTA CTTTTAGAAG TCACGGTAAC TACTTGCACG TCTCAGTAGG AGCAAGTCGA 
GTGGTGGTCG GTGGCAGTAT CAGTGTCCAA GTGTACATCA AATCCTCTTT ACCAAACCAC 
AGAGCCCTCG TGGAGCATCT CACATACATT GTGTTAAATA AGGGGAAAAT AATTCAGGCT 
GGTAGAGTGA ATGTCAAAGG CCAGGACGTA ACAAGCGTTC ATCTGCTCAT TACCTCAGAA 
ATGCTACCTG CATTTCGGTT TGTGGCGTAT TACGTTTTGC CATGGCAGCA TAGGGCTGAA 
GTGGTGGCAG ACTCAGTGTT TGTTGATGTG GAGGACCGGT GTGTAGGTTC GCTTAATGTT 
GGACCAGTGG ATGGAGAAAT AGTCAGTTCT TACTCACCAG GCAGCAGCTT TAAATTTCAA 
GTGAAAGGTG ATGTTGGAGC AAAGGTCAGC CTGGTAGCTG TGGATAATGC CATCTTCCTG 
TTGAGTAAAA ACCGCCTGAC GCAGAAAAAG GTGTGGGAAG TGGTGGATCA GGCTGATATG 
GGCTGCACCG CAGGAGGAGG CAAGGATAAT ATGGGTGTTT TCAGTGATGC AGGACTGATG 
TTTCACTCCA GCACAGGAGG TTCAACTGAC TCCAAAGAAG ACTGCTCCAG TAAAACTAAA 
CGGCGAAGAC GTTCTGCAGC CAAACTGCAG CTCAGAGATC AGCTCGAGAA GGCCTATTCA 
GATGAGCTCC TGCAGAGGTG TTGTGTGGAC GGCATGAAGG AGATTCCCAT GCCTTATTCA 
TGCTACCGTC GTTCCCTCTA CATCACCGAG GACTGGAGCT GTGTGTTGGC TTTCCTGCGC 
TGCTGTGCAG AATACAGAGG AGAGGAGCTC GGCGTCATTA CCAGACCTCC AACAACCACT 
GCTCCACCAA CCACTGCTCC GCCAACCACT TCTCTACCCA CCCTCTTTGA CTTACCATAT 
TTAGAATATG ATTATATGGA AGAAAAGGTT ATATTGAGAG CGAGAATGAT AGAACCCATG 
CCAAAGCTGT ATGCCAGAGC TAATGATGTC GGAGTTCGTC AGGACAGTTA TCGTGAAGTC 
GTTGTAGAAG AAGAGGAAGA AGAGGCGGCT TCAGTGTTTG AGGAAGATGA TTTGTCTGAT 
CTAGAAGATA TTTATGTGCG CAGCAAGTTT TTTGAGTCGT GGTTGTGGAC CGATATCAGA 
CTGCCTAGCA CTGCCAAGTC TGATGGGTTG GCTGTGTTTC CAGTGGACAC CGTTCTTCCA 
GACAGTATCA CACAGTGGGG GTTTCTAGCT GTCAGTGCCT CACCTGAGAA AGGTGTCTGC 
GTTGCTGAAC CGTATAACGT CCGCGTGTGG AAGCCGTTCT TCATCGACCT GCGTTTGCCT 
CACTCAGTGT CCAGAAATGA ACAAGTGGAA ATCAAAGCTG TAATCCACAA CTACAAGAAC 
TCAACACTGG AGGTGATGGT GATCCTGGAG AAGACAGAGG ACATGTGTAG TGTGGCCTAT 
ACGGGACAGC ACAGACAGCA GGTTACAGTA GCAGCCGGTT CCTCTAAACT CATTACATAC 
ACCATCATCC CTCTCAAAAC AGGAGAGCTT CCTCTACAGG TCACTGCTGT CGCTGCCTCA 
TTCATGGGTC AAGATGCAGT GAAGAAGAAT CTACGCGTAG TAGTTGAAGG CATTCAGACA 
CTTAAAGTGA GGAGCTTTGT GTTGAACCCA TCAGAGAAAG GAGGCAGTGC TGGGCAACAG 
TTGATTCAAG TCAAGAAGCT GCAGTTGGAT GCTGTTGTGC CGAAATCTGT ACCAGAAACA 
TTTGTCAATG TCAGAGGTGA TTTGCTGGCA GACAGTATTG ATAACTCCAT TAAGGAGGAC 
TCATTAGCAG CGTTGATCCG GATGCCCGGA GGCTGTGTGG AGCAGAACCT GGCCAGAATC 
ACTCTGCCCC TCATCGCAGC TCATTACCTG GACCGCAGTG CAGACTGGGA GATTGTGGGA 
ATCAGTCGCA GAGAAGAGGC CATCAAATAC ATACAGAAAG GTTATGAAAA TCAACTTAAC 
TACAAAAAAT CAGACGACTC GTACCCTCCC TACGCCAATG AAGGCACCAG CACATGGATC 
ACTGCGTATG TGGTGAAGGT TTTCTCCATG GCGCATTCGT TCATTACCGT CAATGAGAAG 
CATCTGTGTG GCCCTCTGCT CTATTTACTC AAGAATAAAC AGGGTTATGA TGGAACTTTT 
CAAGAGGACA ATCCTGTTTA TGACACCAGT ATGACGGGAG CTATGCGGGG ATCTGAATAC 
AAAGCATCTT TGACAGCATT TGTACTCATT GCTTTAGCAG AGGCACAAGA TAAAGTTACT 
TGCCAAGATC AAGAAGTCAA CATACGGGAT AAGTCTAGAC AGGCAGCAGG GTATCTGACA 
GAGCATTTCT CCCGGTTGAC CAGGCCATAT ACAGCAGCTA TAGCATGCTA CGCGCTAGCC 
GTATCGAACA ACGCCTGCGT GAAGAGCATG TTGCTCAAAT TTGCCTCACC GGATCATTCC 
CACTGGCCTG ATTCAAACAA TTATTTTTTC ACACTGGAAG CTACAGGCTA CGCACTGCTA 
GCGCTGATCA AAAGTGGCCA CATGGAAGAG GCAGAAGCTC CATTTCGATG GCTGAATGAA 
CACCGTGGCA TTGGTGGAGG TTACGGCTCT ACTCAGTCCA CCATGGTGGT TCTTCAGGCC 
CTGTCTGAGT ATCTAGTGAA GAGGCCTCCT CCGTCAGACC TCAATTTGTT GGTTCAGCTC 
AGCGTTCCTG GTCGGAGTGA TGTTCGCTGG ACCTTCAATC CCAATTTGGC TTATGTGGCC 
CGTTCTTCAC GAGTCCCTCT TGATCAGGAA TTCTCAGTGG CGGCTTCTGG GAACGGCAAG 
GGTGTTTTGG AGGTAGTGAC GGTCTACCAT CAGCTTCCTG ATGTGTATGA GAACAGCACA 
TGCAACGGCT TTCAGCTGGA CGTCTCTATC GCTGAGACTA ATGAAAAAAC CTCATCGGAT 
GTAGAAAAGT CCTTCAGACT CAATGTCAAT GTGAGGGCTC TGGAAGAACG GCAAGTGAGG 
ATGGTTATTC TAGACATCGG CCTGCCTACA GGCTTTGAAC CTGAAAACTC TGATTTAGAG 
TTGCTCACAA ATTCAGTGGA TCGCTACATC AACAACTTCC AAGTGGTGGA CAACCTGAGC 
GACAGAGGAT CCCTCATTAT TCACCTGTTC AAGGTGTCCA ACAAAGAGGC AGAAATCATC 
TCATTCAGAC TCAATCAGAG GTTTAAAGTG GGGCTCCTTC AGCCTTCCAC AGTCAAAGTT 
TACCAGTACT ACAACAAAGA TAAAAGCTGT AGCAGGTTCT ACTCTCCTCC TGAAGACAAA 
CAGCAGCTGG ATCAGATCTG TGAAGGCGAC GTGTGCCGCT GCTCGCAGGG AGACTGTTGT 
GTAATGAAGG AAGACTCCGC GTCTTTTGGT AAAGAGCAGA GGAATGCCGC TGTATGCAAC 
GGATTACATC ATGGTGAATA TTTCTTTGGG TCTAATTACA GTGGTTTATT ATTCCTGTCA 
ATGAACAACT TTTCTTCAGC CTACAGAGTC AAGCTGATCA GCATCAGCCA GAGTCACTAT 
TACCAGTATG AGATGGAGAT TGTGCAGGTT ATTAAAGAAG GGACGGAGGA AGGTCTGAAT 
GAGAAGGAGA GGAGGATGTT CCTCTCTCAT GCGAGCTGCA GGACCAGGCT TAATTTGAGA 
GAGGGTCAGG AGTATCTGGT CATTGGGCCG ATCACTGATG TTTGGAATGC AGGAAGCACA 
GTGTCCAACA AGTATGTGTA TACGCTTGGG AAAGAAACGT GGGTTGAGCG CTGGCCAGAT 
GAGTCTGAAT GTGGATCAAC ACTGCAGAAG AAATGTGACG AGCTGAAGAG CTTTGCAAAG 
AAACTGATTG AAGACGGCTG TCAGGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294666.1
CDS	171..5480
Translation	MLLQLLLWATLLCQICDGKAFPQRHKRTVWGPNMRQIEPVKDPSPSFSLLAPAKIRPGTKQNILVEGHNLAKVVKVTVVVYDYPNSQTALSRGSVSLNSDNNYSVLQTLEINPNLLLPDGKKKYVKLVAQFSNIYRAEKVMAVSFRSGHIFIQTDKPIYNPGDKVRFRAFVSDLEFRSSERTISLEIQNPDGIAVHGIGRVKAIDGVFSDIFSLSTVVKEGRWKVVAKFDQGKENVFSREFEVKKYVLPAFNVTLTPKTSHLSLDAEKLEVEITARYLYGAQVKGVAYVLFGIEINGEKKRLTSMKQIDDLNGGSVSLTMEEIKRAYPDINSLLGSTVYVKASVLTSSGSDLVEAEKSGIKIVNSPYMLTIKDTPKYYKPGLPLAMTVIVSNHDGSPTPNIPVKVTFLKRPISVHSGTINIHINMPKKRIPYGNSQMLKVETVDPSLKPEQQAIQEVYVKPYSTFRSHGNYLHVSVGASRVVVGGSISVQVYIKSSLPNHRALVEHLTYIVLNKGKIIQAGRVNVKGQDVTSVHLLITSEMLPAFRFVAYYVLPWQHRAEVVADSVFVDVEDRCVGSLNVGPVDGEIVSSYSPGSSFKFQVKGDVGAKVSLVAVDNAIFLLSKNRLTQKKVWEVVDQADMGCTAGGGKDNMGVFSDAGLMFHSSTGGSTDSKEDCSSKTKRRRRSAAKLQLRDQLEKAYSDELLQRCCVDGMKEIPMPYSCYRRSLYITEDWSCVLAFLRCCAEYRGEELGVITRPPTTTAPPTTAPPTTSLPTLFDLPYLEYDYMEEKVILRARMIEPMPKLYARANDVGVRQDSYREVVVEEEEEEAASVFEEDDLSDLEDIYVRSKFFESWLWTDIRLPSTAKSDGLAVFPVDTVLPDSITQWGFLAVSASPEKGVCVAEPYNVRVWKPFFIDLRLPHSVSRNEQVEIKAVIHNYKNSTLEVMVILEKTEDMCSVAYTGQHRQQVTVAAGSSKLITYTIIPLKTGELPLQVTAVAASFMGQDAVKKNLRVVVEGIQTLKVRSFVLNPSEKGGSAGQQLIQVKKLQLDAVVPKSVPETFVNVRGDLLADSIDNSIKEDSLAALIRMPGGCVEQNLARITLPLIAAHYLDRSADWEIVGISRREEAIKYIQKGYENQLNYKKSDDSYPPYANEGTSTWITAYVVKVFSMAHSFITVNEKHLCGPLLYLLKNKQGYDGTFQEDNPVYDTSMTGAMRGSEYKASLTAFVLIALAEAQDKVTCQDQEVNIRDKSRQAAGYLTEHFSRLTRPYTAAIACYALAVSNNACVKSMLLKFASPDHSHWPDSNNYFFTLEATGYALLALIKSGHMEEAEAPFRWLNEHRGIGGGYGSTQSTMVVLQALSEYLVKRPPPSDLNLLVQLSVPGRSDVRWTFNPNLAYVARSSRVPLDQEFSVAASGNGKGVLEVVTVYHQLPDVYENSTCNGFQLDVSIAETNEKTSSDVEKSFRLNVNVRALEERQVRMVILDIGLPTGFEPENSDLELLTNSVDRYINNFQVVDNLSDRGSLIIHLFKVSNKEAEIISFRLNQRFKVGLLQPSTVKVYQYYNKDKSCSRFYSPPEDKQQLDQICEGDVCRCSQGDCCVMKEDSASFGKEQRNAAVCNGLHHGEYFFGSNYSGLLFLSMNNFSSAYRVKLISISQSHYYQYEMEIVQVIKEGTEEGLNEKERRMFLSHASCRTRLNLREGQEYLVIGPITDVWNAGSTVSNKYVYTLGKETWVERWPDESECGSTLQKKCDELKSFAKKLIEDGCQA

Target ORF information:

RefSeq Version	XM_009296391.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio complement component c3b, tandem duplicate 2 (c3b.2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296391.3

ORF Insert Sequence:

ATGCTTCTTC AGCTGCTGTT ATGGGCGACC CTGCTCTGTC AAATCTGCGA TGGAAAGGCC 
TTCCCTCAAC GACACAAGAG GACGGTTTGG GGACCTAATA TGCGACAAAT TGAGCCGGTC 
AAAGACCCTT CTCCGTCGTT TTCTCTTCTA GCTCCAGCAA AAATAAGGCC TGGAACAAAA 
CAGAACATCC TGGTGGAGGG ACACAACCTG GCAAAGGTGG TAAAAGTCAC TGTTGTGGTT 
TACGACTATC CAAACTCCCA AACTGCCTTG TCACGGGGCT CAGTTTCCCT CAATTCAGAC 
AACAACTACA GCGTCCTGCA AACCCTAGAG ATCAACCCAA ATTTGCTACT TCCAGATGGA 
AAAAAGAAGT ATGTGAAGCT TGTTGCTCAG TTCAGCAACA TCTATCGTGC AGAAAAAGTA 
ATGGCGGTGT CATTTCGCTC TGGACACATC TTCATTCAGA CCGACAAACC TATTTACAAC 
CCTGGAGACA AAGTGCGTTT CAGGGCTTTT GTGTCTGATT TGGAGTTTCG TTCTTCTGAG 
AGAACCATAT CTCTGGAGAT TCAGAATCCA GATGGAATTG CGGTGCATGG TATTGGGAGA 
GTTAAAGCAA TCGATGGGGT CTTTTCTGAC ATCTTCTCAC TGTCTACAGT AGTCAAGGAG 
GGCAGATGGA AAGTTGTAGC AAAGTTTGAT CAGGGAAAAG AGAATGTTTT CAGCAGAGAG 
TTTGAAGTGA AGAAATATGT GCTTCCAGCA TTCAACGTCA CACTTACTCC TAAAACATCC 
CACCTCAGTC TGGATGCAGA AAAGTTGGAA GTTGAAATAA CAGCCAGGTA TCTTTATGGC 
GCGCAAGTGA AGGGTGTTGC GTATGTGCTG TTTGGTATTG AAATTAATGG TGAAAAGAAG 
AGACTCACAT CTATGAAGCA AATTGATGAT CTAAATGGAG GAAGTGTTAG TTTGACTATG 
GAGGAGATAA AGAGAGCTTA TCCTGACATA AACAGCCTCC TGGGGTCTAC GGTGTACGTC 
AAAGCATCAG TATTGACTAG TTCAGGTAGC GATCTTGTGG AAGCAGAGAA ATCCGGCATT 
AAAATCGTAA ATTCGCCCTA CATGCTTACC ATCAAGGACA CACCAAAGTA TTATAAACCT 
GGTCTGCCGT TAGCCATGAC GGTTATCGTG AGTAACCATG ATGGATCTCC AACTCCTAAT 
ATTCCTGTAA AAGTCACCTT TCTTAAAAGG CCAATCAGTG TCCACAGTGG CACCATCAAC 
ATTCACATCA ACATGCCAAA AAAGCGCATC CCATACGGCA ATTCCCAGAT GTTGAAAGTG 
GAGACAGTGG ATCCGTCACT GAAACCAGAA CAGCAGGCCA TTCAAGAGGT ATACGTCAAG 
CCGTATTCTA CTTTTAGAAG TCACGGTAAC TACTTGCACG TCTCAGTAGG AGCAAGTCGA 
GTGGTGGTCG GTGGCAGTAT CAGTGTCCAA GTGTACATCA AATCCTCTTT ACCAAACCAC 
AGAGCCCTCG TGGAGCATCT CACATACATT GTGTTAAATA AGGGGAAAAT AATTCAGGCT 
GGTAGAGTGA ATGTCAAAGG CCAGGACGTA ACAAGCGTTC ATCTGCTCAT TACCTCAGAA 
ATGCTACCTG CATTTCGGTT TGTGGCGTAT TACGTTTTGC CATGGCAGCA TAGGGCTGAA 
GTGGTGGCAG ACTCAGTGTT TGTTGATGTG GAGGACCGGT GTGTAGGTTC GCTTAATGTT 
GGACCAGTGG ATGGAGAAAT AGTCAGTTCT TACTCACCAG GCAGCAGCTT TAAATTTCAA 
GTGAAAGGTG ATGTTGGAGC AAAGGTCAGC CTGGTAGCTG TGGATAATGC CATCTTCCTG 
TTGAGTAAAA ACCGCCTGAC GCAGAAAAAG GTGTGGGAAG TGGTGGATCA GGCTGATATG 
GGCTGCACCG CAGGAGGAGG CAAGGATAAT ATGGGTGTTT TCAGTGATGC AGGACTGATG 
TTTCACTCCA GCACAGGAGG TTCAACTGAC TCCAAAGAAG ACTGCTCCAG TAAAACTAAA 
CGGCGAAGAC GTTCTGCAGC CAAACTGCAG CTCAGAGATC AGCTCGAGAA GGCCTATTCA 
GATGAGCTCC TGCAGAGGTG TTGTGTGGAC GGCATGAAGG AGATTCCCAT GCCTTATTCA 
TGCTACCGTC GTTCCCTCTA CATCACCGAG GACTGGAGCT GTGTGTTGGC TTTCCTGCGC 
TGCTGTGCAG AATACAGAGG AGAGGAGCTC GGCGTCATTA CCAGACCTCC AACAACCACT 
GCTCCACCAA CCACTGCTCC GCCAACCACT TCTCTACCCA CCCTCTTTGA CTTACCATAT 
TTAGAATATG ATTATATGGA AGAAAAGGTT ATATTGAGAG CGAGAATGAT AGAACCCATG 
CCAAAGCTGT ATGCCAGAGC TAATGATGTC GGAGTTCGTC AGGACAGTTA TCGTGAAGTC 
GTTGTAGAAG AAGAGGAAGA AGAGGCGGCT TCAGTGTTTG AGGAAGATGA TTTGTCTGAT 
CTAGAAGATA TTTATGTGCG CAGCAAGTTT TTTGAGTCGT GGTTGTGGAC CGATATCAGA 
CTGCCTAGCA CTGCCAAGTC TGATGGGTTG GCTGTGTTTC CAGTGGACAC CGTTCTTCCA 
GACAGTATCA CACAGTGGGG GTTTCTAGCT GTCAGTGCCT CACCTGAGAA AGGTGTCTGC 
GTTGCTGAAC CGTATAACGT CCGCGTGTGG AAGCCGTTCT TCATCGACCT GCGTTTGCCT 
CACTCAGTGT CCAGAAATGA ACAAGTGGAA ATCAAAGCTG TAATCCACAA CTACAAGAAC 
TCAACACTGG AGGTGATGGT GATCCTGGAG AAGACAGAGG ACATGTGTAG TGTGGCCTAT 
ACGGGACAGC ACAGACAGCA GGTTACAGTA GCAGCCGGTT CCTCTAAACT CATTACATAC 
ACCATCATCC CTCTCAAAAC AGGAGAGCTT CCTCTACAGG TCACTGCTGT CGCTGCCTCA 
TTCATGGGTC AAGATGCAGT GAAGAAGAAT CTACGCGTAG TAGTTGAAGG CATTCAGACA 
CTTAAAGTGA GGAGCTTTGT GTTGAACCCA TCAGAGAAAG GAGGCAGTGC TGGGCAACAG 
TTGATTCAAG TCAAGAAGCT GCAGTTGGAT GCTGTTGTGC CGAAATCTGT ACCAGAAACA 
TTTGTCAATG TCAGAGGTGA TTTGCTGGCA GACAGTATTG ATAACTCCAT TAAGGAGGAC 
TCATTAGCAG CGTTGATCCG GATGCCCGGA GGCTGTGTGG AGCAGAACCT GGCCAGAATC 
ACTCTGCCCC TCATCGCAGC TCATTACCTG GACCGCAGTG CAGACTGGGA GATTGTGGGA 
ATCAGTCGCA GAGAAGAGGC CATCAAATAC ATACAGAAAG GTTATGAAAA TCAACTTAAC 
TACAAAAAAT CAGACGACTC GTACCCTCCC TACGCCAATG AAGGCACCAG CACATGGATC 
ACTGCGTATG TGGTGAAGGT TTTCTCCATG GCGCATTCGT TCATTACCGT CAATGAGAAG 
CATCTGTGTG GCCCTCTGCT CTATTTACTC AAGAATAAAC AGGGTTATGA TGGAACTTTT 
CAAGAGGACA ATCCTGTTTA TGACACCAGT ATGACGGGAG CTATGCGGGG ATCTGAATAC 
AAAGCATCTT TGACAGCATT TGTACTCATT GCTTTAGCAG AGGCACAAGA TAAAGTTACT 
TGCCAAGATC AAGAAGTCAA CATACGGGAT AAGTCTAGAC AGGCAGCAGG GTATCTGACA 
GAGCATTTCT CCCGGTTGAC CAGGCCATAT ACAGCAGCTA TAGCATGCTA CGCGCTAGCC 
GTATCGAACA ACGCCTGCGT GAAGAGCATG TTGCTCAAAT TTGCCTCACC GGATCATTCC 
CACTGGCCTG ATTCAAACAA TTATTTTTTC ACACTGGAAG CTACAGGCTA CGCACTGCTA 
GCGCTGATCA AAAGTGGCCA CATGGAAGAG GCAGAAGCTC CATTTCGATG GCTGAATGAA 
CACCGTGGCA TTGGTGGAGG TTACGGCTCT ACTCAGTCCA CCATGGTGGT TCTTCAGGCC 
CTGTCTGAGT ATCTAGTGAA GAGGCCTCCT CCGTCAGACC TCAATTTGTT GGTTCAGCTC 
AGCGTTCCTG GTCGGAGTGA TGTTCGCTGG ACCTTCAATC CCAATTTGGC TTATGTGGCC 
CGTTCTTCAC GAGTCCCTCT TGATCAGGAA TTCTCAGTGG CGGCTTCTGG GAACGGCAAG 
GGTGTTTTGG AGGTAGTGAC GGTCTACCAT CAGCTTCCTG ATGTGTATGA GAACAGCACA 
TGCAACGGCT TTCAGCTGGA CGTCTCTATC GCTGAGACTA ATGAAAAAAC CTCATCGGAT 
GTAGAAAAGT CCTTCAGACT CAATGTCAAT GTGAGGGCTC TGGAAGAACG GCAAGTGAGG 
ATGGTTATTC TAGACATCGG CCTGCCTACA GGCTTTGAAC CTGAAAACTC TGATTTAGAG 
TTGCTCACAA ATTCAGTGGA TCGCTACATC AACAACTTCC AAGTGGTGGA CAACCTGAGC 
GACAGAGGAT CCCTCATTAT TCACCTGTTC AAGGTGTCCA ACAAAGAGGC AGAAATCATC 
TCATTCAGAC TCAATCAGAG GTTTAAAGTG GGGCTCCTTC AGCCTTCCAC AGTCAAAGTT 
TACCAGTACT ACAACAAAGA TAAAAGCTGT AGCAGGTTCT ACTCTCCTCC TGAAGACAAA 
CAGCAGCTGG ATCAGATCTG TGAAGGCGAC GTGTGCCGCT GCTCGCAGGG AGACTGTTGT 
GTAATGAAGG AAGACTCCGC GTCTTTTGGT AAAGAGCAGA GGAATGCCGC TGTATGCAAC 
GGATTACATC ATGGTGAATA TTTCTTTGGG TCTAATTACA GTGGTTTATT ATTCCTGTCA 
ATGAACAACT TTTCTTCAGC CTACAGAGTC AAGCTGATCA GCATCAGCCA GAGTCACTAT 
TACCAGTATG AGATGGAGAT TGTGCAGGTT ATTAAAGAAG GGACGGAGGA AGGTCTGAAT 
GAGAAGGAGA GGAGGATGTT CCTCTCTCAT GCGAGCTGCA GGACCAGGCT TAATTTGAGA 
GAGGGTCAGG AGTATCTGGT CATTGGGCCG ATCACTGATG TTTGGAATGC AGGAAGCACA 
GTGTCCAACA AGTATGTGTA TACGCTTGGG AAAGAAACGT GGGTTGAGCG CTGGCCAGAT 
GAGTCTGAAT GTGGATCAAC ACTGCAGAAG AAATGTGACG AGCTGAAGAG CTTTGCAAAG 
AAACTGATTG AAGACGGCTG TCAGGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

c3b.2 ( XM_009296391.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009294666.1 Danio rerio complement component c3b, tandem duplicate 2 (c3b.2), transcript variant X1, mRNA.

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CloneID	ODa44848
Clone ID Related Accession (Same CDS sequence)	XM_009296391.3 , XM_009296391.2
Accession Version	XM_009296391.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5310bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	complement component c3b, tandem duplicate 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296391.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCTTC AGCTGCTGTT ATGGGCGACC CTGCTCTGTC AAATCTGCGA TGGAAAGGCC 
TTCCCTCAAC GACACAAGAG GACGGTTTGG GGACCTAATA TGCGACAAAT TGAGCCGGTC 
AAAGACCCTT CTCCGTCGTT TTCTCTTCTA GCTCCAGCAA AAATAAGGCC TGGAACAAAA 
CAGAACATCC TGGTGGAGGG ACACAACCTG GCAAAGGTGG TAAAAGTCAC TGTTGTGGTT 
TACGACTATC CAAACTCCCA AACTGCCTTG TCACGGGGCT CAGTTTCCCT CAATTCAGAC 
AACAACTACA GCGTCCTGCA AACCCTAGAG ATCAACCCAA ATTTGCTACT TCCAGATGGA 
AAAAAGAAGT ATGTGAAGCT TGTTGCTCAG TTCAGCAACA TCTATCGTGC AGAAAAAGTA 
ATGGCGGTGT CATTTCGCTC TGGACACATC TTCATTCAGA CCGACAAACC TATTTACAAC 
CCTGGAGACA AAGTGCGTTT CAGGGCTTTT GTGTCTGATT TGGAGTTTCG TTCTTCTGAG 
AGAACCATAT CTCTGGAGAT TCAGAATCCA GATGGAATTG CGGTGCATGG TATTGGGAGA 
GTTAAAGCAA TCGATGGGGT CTTTTCTGAC ATCTTCTCAC TGTCTACAGT AGTCAAGGAG 
GGCAGATGGA AAGTTGTAGC AAAGTTTGAT CAGGGAAAAG AGAATGTTTT CAGCAGAGAG 
TTTGAAGTGA AGAAATATGT GCTTCCAGCA TTCAACGTCA CACTTACTCC TAAAACATCC 
CACCTCAGTC TGGATGCAGA AAAGTTGGAA GTTGAAATAA CAGCCAGGTA TCTTTATGGC 
GCGCAAGTGA AGGGTGTTGC GTATGTGCTG TTTGGTATTG AAATTAATGG TGAAAAGAAG 
AGACTCACAT CTATGAAGCA AATTGATGAT CTAAATGGAG GAAGTGTTAG TTTGACTATG 
GAGGAGATAA AGAGAGCTTA TCCTGACATA AACAGCCTCC TGGGGTCTAC GGTGTACGTC 
AAAGCATCAG TATTGACTAG TTCAGGTAGC GATCTTGTGG AAGCAGAGAA ATCCGGCATT 
AAAATCGTAA ATTCGCCCTA CATGCTTACC ATCAAGGACA CACCAAAGTA TTATAAACCT 
GGTCTGCCGT TAGCCATGAC GGTTATCGTG AGTAACCATG ATGGATCTCC AACTCCTAAT 
ATTCCTGTAA AAGTCACCTT TCTTAAAAGG CCAATCAGTG TCCACAGTGG CACCATCAAC 
ATTCACATCA ACATGCCAAA AAAGCGCATC CCATACGGCA ATTCCCAGAT GTTGAAAGTG 
GAGACAGTGG ATCCGTCACT GAAACCAGAA CAGCAGGCCA TTCAAGAGGT ATACGTCAAG 
CCGTATTCTA CTTTTAGAAG TCACGGTAAC TACTTGCACG TCTCAGTAGG AGCAAGTCGA 
GTGGTGGTCG GTGGCAGTAT CAGTGTCCAA GTGTACATCA AATCCTCTTT ACCAAACCAC 
AGAGCCCTCG TGGAGCATCT CACATACATT GTGTTAAATA AGGGGAAAAT AATTCAGGCT 
GGTAGAGTGA ATGTCAAAGG CCAGGACGTA ACAAGCGTTC ATCTGCTCAT TACCTCAGAA 
ATGCTACCTG CATTTCGGTT TGTGGCGTAT TACGTTTTGC CATGGCAGCA TAGGGCTGAA 
GTGGTGGCAG ACTCAGTGTT TGTTGATGTG GAGGACCGGT GTGTAGGTTC GCTTAATGTT 
GGACCAGTGG ATGGAGAAAT AGTCAGTTCT TACTCACCAG GCAGCAGCTT TAAATTTCAA 
GTGAAAGGTG ATGTTGGAGC AAAGGTCAGC CTGGTAGCTG TGGATAATGC CATCTTCCTG 
TTGAGTAAAA ACCGCCTGAC GCAGAAAAAG GTGTGGGAAG TGGTGGATCA GGCTGATATG 
GGCTGCACCG CAGGAGGAGG CAAGGATAAT ATGGGTGTTT TCAGTGATGC AGGACTGATG 
TTTCACTCCA GCACAGGAGG TTCAACTGAC TCCAAAGAAG ACTGCTCCAG TAAAACTAAA 
CGGCGAAGAC GTTCTGCAGC CAAACTGCAG CTCAGAGATC AGCTCGAGAA GGCCTATTCA 
GATGAGCTCC TGCAGAGGTG TTGTGTGGAC GGCATGAAGG AGATTCCCAT GCCTTATTCA 
TGCTACCGTC GTTCCCTCTA CATCACCGAG GACTGGAGCT GTGTGTTGGC TTTCCTGCGC 
TGCTGTGCAG AATACAGAGG AGAGGAGCTC GGCGTCATTA CCAGACCTCC AACAACCACT 
GCTCCACCAA CCACTGCTCC GCCAACCACT TCTCTACCCA CCCTCTTTGA CTTACCATAT 
TTAGAATATG ATTATATGGA AGAAAAGGTT ATATTGAGAG CGAGAATGAT AGAACCCATG 
CCAAAGCTGT ATGCCAGAGC TAATGATGTC GGAGTTCGTC AGGACAGTTA TCGTGAAGTC 
GTTGTAGAAG AAGAGGAAGA AGAGGCGGCT TCAGTGTTTG AGGAAGATGA TTTGTCTGAT 
CTAGAAGATA TTTATGTGCG CAGCAAGTTT TTTGAGTCGT GGTTGTGGAC CGATATCAGA 
CTGCCTAGCA CTGCCAAGTC TGATGGGTTG GCTGTGTTTC CAGTGGACAC CGTTCTTCCA 
GACAGTATCA CACAGTGGGG GTTTCTAGCT GTCAGTGCCT CACCTGAGAA AGGTGTCTGC 
GTTGCTGAAC CGTATAACGT CCGCGTGTGG AAGCCGTTCT TCATCGACCT GCGTTTGCCT 
CACTCAGTGT CCAGAAATGA ACAAGTGGAA ATCAAAGCTG TAATCCACAA CTACAAGAAC 
TCAACACTGG AGGTGATGGT GATCCTGGAG AAGACAGAGG ACATGTGTAG TGTGGCCTAT 
ACGGGACAGC ACAGACAGCA GGTTACAGTA GCAGCCGGTT CCTCTAAACT CATTACATAC 
ACCATCATCC CTCTCAAAAC AGGAGAGCTT CCTCTACAGG TCACTGCTGT CGCTGCCTCA 
TTCATGGGTC AAGATGCAGT GAAGAAGAAT CTACGCGTAG TAGTTGAAGG CATTCAGACA 
CTTAAAGTGA GGAGCTTTGT GTTGAACCCA TCAGAGAAAG GAGGCAGTGC TGGGCAACAG 
TTGATTCAAG TCAAGAAGCT GCAGTTGGAT GCTGTTGTGC CGAAATCTGT ACCAGAAACA 
TTTGTCAATG TCAGAGGTGA TTTGCTGGCA GACAGTATTG ATAACTCCAT TAAGGAGGAC 
TCATTAGCAG CGTTGATCCG GATGCCCGGA GGCTGTGTGG AGCAGAACCT GGCCAGAATC 
ACTCTGCCCC TCATCGCAGC TCATTACCTG GACCGCAGTG CAGACTGGGA GATTGTGGGA 
ATCAGTCGCA GAGAAGAGGC CATCAAATAC ATACAGAAAG GTTATGAAAA TCAACTTAAC 
TACAAAAAAT CAGACGACTC GTACCCTCCC TACGCCAATG AAGGCACCAG CACATGGATC 
ACTGCGTATG TGGTGAAGGT TTTCTCCATG GCGCATTCGT TCATTACCGT CAATGAGAAG 
CATCTGTGTG GCCCTCTGCT CTATTTACTC AAGAATAAAC AGGGTTATGA TGGAACTTTT 
CAAGAGGACA ATCCTGTTTA TGACACCAGT ATGACGGGAG CTATGCGGGG ATCTGAATAC 
AAAGCATCTT TGACAGCATT TGTACTCATT GCTTTAGCAG AGGCACAAGA TAAAGTTACT 
TGCCAAGATC AAGAAGTCAA CATACGGGAT AAGTCTAGAC AGGCAGCAGG GTATCTGACA 
GAGCATTTCT CCCGGTTGAC CAGGCCATAT ACAGCAGCTA TAGCATGCTA CGCGCTAGCC 
GTATCGAACA ACGCCTGCGT GAAGAGCATG TTGCTCAAAT TTGCCTCACC GGATCATTCC 
CACTGGCCTG ATTCAAACAA TTATTTTTTC ACACTGGAAG CTACAGGCTA CGCACTGCTA 
GCGCTGATCA AAAGTGGCCA CATGGAAGAG GCAGAAGCTC CATTTCGATG GCTGAATGAA 
CACCGTGGCA TTGGTGGAGG TTACGGCTCT ACTCAGTCCA CCATGGTGGT TCTTCAGGCC 
CTGTCTGAGT ATCTAGTGAA GAGGCCTCCT CCGTCAGACC TCAATTTGTT GGTTCAGCTC 
AGCGTTCCTG GTCGGAGTGA TGTTCGCTGG ACCTTCAATC CCAATTTGGC TTATGTGGCC 
CGTTCTTCAC GAGTCCCTCT TGATCAGGAA TTCTCAGTGG CGGCTTCTGG GAACGGCAAG 
GGTGTTTTGG AGGTAGTGAC GGTCTACCAT CAGCTTCCTG ATGTGTATGA GAACAGCACA 
TGCAACGGCT TTCAGCTGGA CGTCTCTATC GCTGAGACTA ATGAAAAAAC CTCATCGGAT 
GTAGAAAAGT CCTTCAGACT CAATGTCAAT GTGAGGGCTC TGGAAGAACG GCAAGTGAGG 
ATGGTTATTC TAGACATCGG CCTGCCTACA GGCTTTGAAC CTGAAAACTC TGATTTAGAG 
TTGCTCACAA ATTCAGTGGA TCGCTACATC AACAACTTCC AAGTGGTGGA CAACCTGAGC 
GACAGAGGAT CCCTCATTAT TCACCTGTTC AAGGTGTCCA ACAAAGAGGC AGAAATCATC 
TCATTCAGAC TCAATCAGAG GTTTAAAGTG GGGCTCCTTC AGCCTTCCAC AGTCAAAGTT 
TACCAGTACT ACAACAAAGA TAAAAGCTGT AGCAGGTTCT ACTCTCCTCC TGAAGACAAA 
CAGCAGCTGG ATCAGATCTG TGAAGGCGAC GTGTGCCGCT GCTCGCAGGG AGACTGTTGT 
GTAATGAAGG AAGACTCCGC GTCTTTTGGT AAAGAGCAGA GGAATGCCGC TGTATGCAAC 
GGATTACATC ATGGTGAATA TTTCTTTGGG TCTAATTACA GTGGTTTATT ATTCCTGTCA 
ATGAACAACT TTTCTTCAGC CTACAGAGTC AAGCTGATCA GCATCAGCCA GAGTCACTAT 
TACCAGTATG AGATGGAGAT TGTGCAGGTT ATTAAAGAAG GGACGGAGGA AGGTCTGAAT 
GAGAAGGAGA GGAGGATGTT CCTCTCTCAT GCGAGCTGCA GGACCAGGCT TAATTTGAGA 
GAGGGTCAGG AGTATCTGGT CATTGGGCCG ATCACTGATG TTTGGAATGC AGGAAGCACA 
GTGTCCAACA AGTATGTGTA TACGCTTGGG AAAGAAACGT GGGTTGAGCG CTGGCCAGAT 
GAGTCTGAAT GTGGATCAAC ACTGCAGAAG AAATGTGACG AGCTGAAGAG CTTTGCAAAG 
AAACTGATTG AAGACGGCTG TCAGGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294666.1
CDS	141..5450
Translation	MLLQLLLWATLLCQICDGKAFPQRHKRTVWGPNMRQIEPVKDPSPSFSLLAPAKIRPGTKQNILVEGHNLAKVVKVTVVVYDYPNSQTALSRGSVSLNSDNNYSVLQTLEINPNLLLPDGKKKYVKLVAQFSNIYRAEKVMAVSFRSGHIFIQTDKPIYNPGDKVRFRAFVSDLEFRSSERTISLEIQNPDGIAVHGIGRVKAIDGVFSDIFSLSTVVKEGRWKVVAKFDQGKENVFSREFEVKKYVLPAFNVTLTPKTSHLSLDAEKLEVEITARYLYGAQVKGVAYVLFGIEINGEKKRLTSMKQIDDLNGGSVSLTMEEIKRAYPDINSLLGSTVYVKASVLTSSGSDLVEAEKSGIKIVNSPYMLTIKDTPKYYKPGLPLAMTVIVSNHDGSPTPNIPVKVTFLKRPISVHSGTINIHINMPKKRIPYGNSQMLKVETVDPSLKPEQQAIQEVYVKPYSTFRSHGNYLHVSVGASRVVVGGSISVQVYIKSSLPNHRALVEHLTYIVLNKGKIIQAGRVNVKGQDVTSVHLLITSEMLPAFRFVAYYVLPWQHRAEVVADSVFVDVEDRCVGSLNVGPVDGEIVSSYSPGSSFKFQVKGDVGAKVSLVAVDNAIFLLSKNRLTQKKVWEVVDQADMGCTAGGGKDNMGVFSDAGLMFHSSTGGSTDSKEDCSSKTKRRRRSAAKLQLRDQLEKAYSDELLQRCCVDGMKEIPMPYSCYRRSLYITEDWSCVLAFLRCCAEYRGEELGVITRPPTTTAPPTTAPPTTSLPTLFDLPYLEYDYMEEKVILRARMIEPMPKLYARANDVGVRQDSYREVVVEEEEEEAASVFEEDDLSDLEDIYVRSKFFESWLWTDIRLPSTAKSDGLAVFPVDTVLPDSITQWGFLAVSASPEKGVCVAEPYNVRVWKPFFIDLRLPHSVSRNEQVEIKAVIHNYKNSTLEVMVILEKTEDMCSVAYTGQHRQQVTVAAGSSKLITYTIIPLKTGELPLQVTAVAASFMGQDAVKKNLRVVVEGIQTLKVRSFVLNPSEKGGSAGQQLIQVKKLQLDAVVPKSVPETFVNVRGDLLADSIDNSIKEDSLAALIRMPGGCVEQNLARITLPLIAAHYLDRSADWEIVGISRREEAIKYIQKGYENQLNYKKSDDSYPPYANEGTSTWITAYVVKVFSMAHSFITVNEKHLCGPLLYLLKNKQGYDGTFQEDNPVYDTSMTGAMRGSEYKASLTAFVLIALAEAQDKVTCQDQEVNIRDKSRQAAGYLTEHFSRLTRPYTAAIACYALAVSNNACVKSMLLKFASPDHSHWPDSNNYFFTLEATGYALLALIKSGHMEEAEAPFRWLNEHRGIGGGYGSTQSTMVVLQALSEYLVKRPPPSDLNLLVQLSVPGRSDVRWTFNPNLAYVARSSRVPLDQEFSVAASGNGKGVLEVVTVYHQLPDVYENSTCNGFQLDVSIAETNEKTSSDVEKSFRLNVNVRALEERQVRMVILDIGLPTGFEPENSDLELLTNSVDRYINNFQVVDNLSDRGSLIIHLFKVSNKEAEIISFRLNQRFKVGLLQPSTVKVYQYYNKDKSCSRFYSPPEDKQQLDQICEGDVCRCSQGDCCVMKEDSASFGKEQRNAAVCNGLHHGEYFFGSNYSGLLFLSMNNFSSAYRVKLISISQSHYYQYEMEIVQVIKEGTEEGLNEKERRMFLSHASCRTRLNLREGQEYLVIGPITDVWNAGSTVSNKYVYTLGKETWVERWPDESECGSTLQKKCDELKSFAKKLIEDGCQA

Target ORF information:

RefSeq Version	XM_009296391.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio complement component c3b, tandem duplicate 2 (c3b.2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296391.2

ORF Insert Sequence:

ATGCTTCTTC AGCTGCTGTT ATGGGCGACC CTGCTCTGTC AAATCTGCGA TGGAAAGGCC 
TTCCCTCAAC GACACAAGAG GACGGTTTGG GGACCTAATA TGCGACAAAT TGAGCCGGTC 
AAAGACCCTT CTCCGTCGTT TTCTCTTCTA GCTCCAGCAA AAATAAGGCC TGGAACAAAA 
CAGAACATCC TGGTGGAGGG ACACAACCTG GCAAAGGTGG TAAAAGTCAC TGTTGTGGTT 
TACGACTATC CAAACTCCCA AACTGCCTTG TCACGGGGCT CAGTTTCCCT CAATTCAGAC 
AACAACTACA GCGTCCTGCA AACCCTAGAG ATCAACCCAA ATTTGCTACT TCCAGATGGA 
AAAAAGAAGT ATGTGAAGCT TGTTGCTCAG TTCAGCAACA TCTATCGTGC AGAAAAAGTA 
ATGGCGGTGT CATTTCGCTC TGGACACATC TTCATTCAGA CCGACAAACC TATTTACAAC 
CCTGGAGACA AAGTGCGTTT CAGGGCTTTT GTGTCTGATT TGGAGTTTCG TTCTTCTGAG 
AGAACCATAT CTCTGGAGAT TCAGAATCCA GATGGAATTG CGGTGCATGG TATTGGGAGA 
GTTAAAGCAA TCGATGGGGT CTTTTCTGAC ATCTTCTCAC TGTCTACAGT AGTCAAGGAG 
GGCAGATGGA AAGTTGTAGC AAAGTTTGAT CAGGGAAAAG AGAATGTTTT CAGCAGAGAG 
TTTGAAGTGA AGAAATATGT GCTTCCAGCA TTCAACGTCA CACTTACTCC TAAAACATCC 
CACCTCAGTC TGGATGCAGA AAAGTTGGAA GTTGAAATAA CAGCCAGGTA TCTTTATGGC 
GCGCAAGTGA AGGGTGTTGC GTATGTGCTG TTTGGTATTG AAATTAATGG TGAAAAGAAG 
AGACTCACAT CTATGAAGCA AATTGATGAT CTAAATGGAG GAAGTGTTAG TTTGACTATG 
GAGGAGATAA AGAGAGCTTA TCCTGACATA AACAGCCTCC TGGGGTCTAC GGTGTACGTC 
AAAGCATCAG TATTGACTAG TTCAGGTAGC GATCTTGTGG AAGCAGAGAA ATCCGGCATT 
AAAATCGTAA ATTCGCCCTA CATGCTTACC ATCAAGGACA CACCAAAGTA TTATAAACCT 
GGTCTGCCGT TAGCCATGAC GGTTATCGTG AGTAACCATG ATGGATCTCC AACTCCTAAT 
ATTCCTGTAA AAGTCACCTT TCTTAAAAGG CCAATCAGTG TCCACAGTGG CACCATCAAC 
ATTCACATCA ACATGCCAAA AAAGCGCATC CCATACGGCA ATTCCCAGAT GTTGAAAGTG 
GAGACAGTGG ATCCGTCACT GAAACCAGAA CAGCAGGCCA TTCAAGAGGT ATACGTCAAG 
CCGTATTCTA CTTTTAGAAG TCACGGTAAC TACTTGCACG TCTCAGTAGG AGCAAGTCGA 
GTGGTGGTCG GTGGCAGTAT CAGTGTCCAA GTGTACATCA AATCCTCTTT ACCAAACCAC 
AGAGCCCTCG TGGAGCATCT CACATACATT GTGTTAAATA AGGGGAAAAT AATTCAGGCT 
GGTAGAGTGA ATGTCAAAGG CCAGGACGTA ACAAGCGTTC ATCTGCTCAT TACCTCAGAA 
ATGCTACCTG CATTTCGGTT TGTGGCGTAT TACGTTTTGC CATGGCAGCA TAGGGCTGAA 
GTGGTGGCAG ACTCAGTGTT TGTTGATGTG GAGGACCGGT GTGTAGGTTC GCTTAATGTT 
GGACCAGTGG ATGGAGAAAT AGTCAGTTCT TACTCACCAG GCAGCAGCTT TAAATTTCAA 
GTGAAAGGTG ATGTTGGAGC AAAGGTCAGC CTGGTAGCTG TGGATAATGC CATCTTCCTG 
TTGAGTAAAA ACCGCCTGAC GCAGAAAAAG GTGTGGGAAG TGGTGGATCA GGCTGATATG 
GGCTGCACCG CAGGAGGAGG CAAGGATAAT ATGGGTGTTT TCAGTGATGC AGGACTGATG 
TTTCACTCCA GCACAGGAGG TTCAACTGAC TCCAAAGAAG ACTGCTCCAG TAAAACTAAA 
CGGCGAAGAC GTTCTGCAGC CAAACTGCAG CTCAGAGATC AGCTCGAGAA GGCCTATTCA 
GATGAGCTCC TGCAGAGGTG TTGTGTGGAC GGCATGAAGG AGATTCCCAT GCCTTATTCA 
TGCTACCGTC GTTCCCTCTA CATCACCGAG GACTGGAGCT GTGTGTTGGC TTTCCTGCGC 
TGCTGTGCAG AATACAGAGG AGAGGAGCTC GGCGTCATTA CCAGACCTCC AACAACCACT 
GCTCCACCAA CCACTGCTCC GCCAACCACT TCTCTACCCA CCCTCTTTGA CTTACCATAT 
TTAGAATATG ATTATATGGA AGAAAAGGTT ATATTGAGAG CGAGAATGAT AGAACCCATG 
CCAAAGCTGT ATGCCAGAGC TAATGATGTC GGAGTTCGTC AGGACAGTTA TCGTGAAGTC 
GTTGTAGAAG AAGAGGAAGA AGAGGCGGCT TCAGTGTTTG AGGAAGATGA TTTGTCTGAT 
CTAGAAGATA TTTATGTGCG CAGCAAGTTT TTTGAGTCGT GGTTGTGGAC CGATATCAGA 
CTGCCTAGCA CTGCCAAGTC TGATGGGTTG GCTGTGTTTC CAGTGGACAC CGTTCTTCCA 
GACAGTATCA CACAGTGGGG GTTTCTAGCT GTCAGTGCCT CACCTGAGAA AGGTGTCTGC 
GTTGCTGAAC CGTATAACGT CCGCGTGTGG AAGCCGTTCT TCATCGACCT GCGTTTGCCT 
CACTCAGTGT CCAGAAATGA ACAAGTGGAA ATCAAAGCTG TAATCCACAA CTACAAGAAC 
TCAACACTGG AGGTGATGGT GATCCTGGAG AAGACAGAGG ACATGTGTAG TGTGGCCTAT 
ACGGGACAGC ACAGACAGCA GGTTACAGTA GCAGCCGGTT CCTCTAAACT CATTACATAC 
ACCATCATCC CTCTCAAAAC AGGAGAGCTT CCTCTACAGG TCACTGCTGT CGCTGCCTCA 
TTCATGGGTC AAGATGCAGT GAAGAAGAAT CTACGCGTAG TAGTTGAAGG CATTCAGACA 
CTTAAAGTGA GGAGCTTTGT GTTGAACCCA TCAGAGAAAG GAGGCAGTGC TGGGCAACAG 
TTGATTCAAG TCAAGAAGCT GCAGTTGGAT GCTGTTGTGC CGAAATCTGT ACCAGAAACA 
TTTGTCAATG TCAGAGGTGA TTTGCTGGCA GACAGTATTG ATAACTCCAT TAAGGAGGAC 
TCATTAGCAG CGTTGATCCG GATGCCCGGA GGCTGTGTGG AGCAGAACCT GGCCAGAATC 
ACTCTGCCCC TCATCGCAGC TCATTACCTG GACCGCAGTG CAGACTGGGA GATTGTGGGA 
ATCAGTCGCA GAGAAGAGGC CATCAAATAC ATACAGAAAG GTTATGAAAA TCAACTTAAC 
TACAAAAAAT CAGACGACTC GTACCCTCCC TACGCCAATG AAGGCACCAG CACATGGATC 
ACTGCGTATG TGGTGAAGGT TTTCTCCATG GCGCATTCGT TCATTACCGT CAATGAGAAG 
CATCTGTGTG GCCCTCTGCT CTATTTACTC AAGAATAAAC AGGGTTATGA TGGAACTTTT 
CAAGAGGACA ATCCTGTTTA TGACACCAGT ATGACGGGAG CTATGCGGGG ATCTGAATAC 
AAAGCATCTT TGACAGCATT TGTACTCATT GCTTTAGCAG AGGCACAAGA TAAAGTTACT 
TGCCAAGATC AAGAAGTCAA CATACGGGAT AAGTCTAGAC AGGCAGCAGG GTATCTGACA 
GAGCATTTCT CCCGGTTGAC CAGGCCATAT ACAGCAGCTA TAGCATGCTA CGCGCTAGCC 
GTATCGAACA ACGCCTGCGT GAAGAGCATG TTGCTCAAAT TTGCCTCACC GGATCATTCC 
CACTGGCCTG ATTCAAACAA TTATTTTTTC ACACTGGAAG CTACAGGCTA CGCACTGCTA 
GCGCTGATCA AAAGTGGCCA CATGGAAGAG GCAGAAGCTC CATTTCGATG GCTGAATGAA 
CACCGTGGCA TTGGTGGAGG TTACGGCTCT ACTCAGTCCA CCATGGTGGT TCTTCAGGCC 
CTGTCTGAGT ATCTAGTGAA GAGGCCTCCT CCGTCAGACC TCAATTTGTT GGTTCAGCTC 
AGCGTTCCTG GTCGGAGTGA TGTTCGCTGG ACCTTCAATC CCAATTTGGC TTATGTGGCC 
CGTTCTTCAC GAGTCCCTCT TGATCAGGAA TTCTCAGTGG CGGCTTCTGG GAACGGCAAG 
GGTGTTTTGG AGGTAGTGAC GGTCTACCAT CAGCTTCCTG ATGTGTATGA GAACAGCACA 
TGCAACGGCT TTCAGCTGGA CGTCTCTATC GCTGAGACTA ATGAAAAAAC CTCATCGGAT 
GTAGAAAAGT CCTTCAGACT CAATGTCAAT GTGAGGGCTC TGGAAGAACG GCAAGTGAGG 
ATGGTTATTC TAGACATCGG CCTGCCTACA GGCTTTGAAC CTGAAAACTC TGATTTAGAG 
TTGCTCACAA ATTCAGTGGA TCGCTACATC AACAACTTCC AAGTGGTGGA CAACCTGAGC 
GACAGAGGAT CCCTCATTAT TCACCTGTTC AAGGTGTCCA ACAAAGAGGC AGAAATCATC 
TCATTCAGAC TCAATCAGAG GTTTAAAGTG GGGCTCCTTC AGCCTTCCAC AGTCAAAGTT 
TACCAGTACT ACAACAAAGA TAAAAGCTGT AGCAGGTTCT ACTCTCCTCC TGAAGACAAA 
CAGCAGCTGG ATCAGATCTG TGAAGGCGAC GTGTGCCGCT GCTCGCAGGG AGACTGTTGT 
GTAATGAAGG AAGACTCCGC GTCTTTTGGT AAAGAGCAGA GGAATGCCGC TGTATGCAAC 
GGATTACATC ATGGTGAATA TTTCTTTGGG TCTAATTACA GTGGTTTATT ATTCCTGTCA 
ATGAACAACT TTTCTTCAGC CTACAGAGTC AAGCTGATCA GCATCAGCCA GAGTCACTAT 
TACCAGTATG AGATGGAGAT TGTGCAGGTT ATTAAAGAAG GGACGGAGGA AGGTCTGAAT 
GAGAAGGAGA GGAGGATGTT CCTCTCTCAT GCGAGCTGCA GGACCAGGCT TAATTTGAGA 
GAGGGTCAGG AGTATCTGGT CATTGGGCCG ATCACTGATG TTTGGAATGC AGGAAGCACA 
GTGTCCAACA AGTATGTGTA TACGCTTGGG AAAGAAACGT GGGTTGAGCG CTGGCCAGAT 
GAGTCTGAAT GTGGATCAAC ACTGCAGAAG AAATGTGACG AGCTGAAGAG CTTTGCAAAG 
AAACTGATTG AAGACGGCTG TCAGGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

The evolution and appearance of C3 duplications in fish originate an exclusive teleost c3 gene form with anti-inflammatory activity.
PloS one9(6)e99673(2014)
Forn-Cun? G,Reis ES,Dios S,Posada D,Lambris JD,Figueras A,Novoa B

Hematopoietic gene expression profile in zebrafish kidney marrow.
Proceedings of the National Academy of Sciences of the United States of America101(46)16240-5(2004 Nov)
Song HD,Sun XJ,Deng M,Zhang GW,Zhou Y,Wu XY,Sheng Y,Chen Y,Ruan Z,Jiang CL,Fan HY,Zon LI,Kanki JP,Liu TX,Look AT,Chen Z

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c3b.2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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