ANKRD52B cDNA ORF clone, Danio rerio(Zebrafish)

The following ANKRD52B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ANKRD52B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa16662 XM_687947.10
Latest version!
Danio rerio ankyrin repeat domain 52b (ankrd52b), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
View Old Accession Versions >>
ODa16662 XM_687947.9 Danio rerio ankyrin repeat domain 52b (ankrd52b), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa16662
    Clone ID Related Accession (Same CDS sequence) XM_687947.10 , XM_687947.9
    Accession Version XM_687947.10 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1899bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit C-like
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_687947.9. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGGGACAC TCAACATTAG CGAGCAGTCT CTCCTGGTTC AGGCAGTGTT CAGCCAGAAT 
    GCTGAAATGC TGGAGTTTTT ATTGGAACAA AATGAAGACG TCAACGAATT GGACCAAGAG
    TGTCGCAGCG CTCTGCACGC CGCTGCGTTT GTGGGTGGTG CTCATATCAT GGACCTCCTC
    ATCAGTTCAG GTGCTAATGT TAATGCTAAA GATCAGGAGT GGTTGACTCC TCTGCATCGA
    GCAGCTGCTT CACAGAATGA AGTGTCTGTG GGCCTGCTGA TGGAAAAGGG AGCAGATGTG
    AACACACAGG ACAGATTCTG CCAGACGCCA TTACATGTAG CTGCAGCCAA CAGAGCCACT
    AAATGTGTGG ATGTTTTGCT TTTTCACGAG AGCAGTTTAA ATGCAGCGGA TTGTTTTGGG
    AGAACAGCTT TACATCACGC AGTGCACAGC GGGCACACAG AGACAGTGGC TGTGCTGCTG
    AACAAAGGGG CCAATGTGAG TGCCAGAGAC ATAAAGGACC GGCAGCCGCT CCATCTGGCC
    GCCTTTCTAG GGAACATGGA GGTCGTGAAG CTGTTGTTGG CGTTCAGAGC TGATGTGTCC
    TGCAGGGATA AACTGGCCTT CACTCCTTTA CATGCGGCAG CTCTTGGCGA ACACACTGAA
    GTGCTCAAAT ACCTGCTGAA ACATGGAGCA CAGATTGATG AAGCGGATGG TTCTGGAAAC
    ACTGCTCTTC ACATGGTGTG CAGTGCTGGT CTGGAGTCAG TAGTGTGTGA CCTGGTGAAC
    TGCGGTGCCG ATGTGAACCG TCCAAACCTG CGCGGCTCCA CACCTCTGCA TCTGTGTGCT
    GAGTCCCCCG ACGGAGCCCT GTGCCTGGAG CTGCTGGTCA ATAACGGAGC ACACGTTAAC
    ATTCCGAATA ATGAAGGGAA GAGTCCTTTG CACATGGCCG CCATTCATGG ACACTTCACC
    CGTTCCCAAA TACTCATTCA GAACGGCGCA GAAGTGGAGT GTGTGGATAA TTATGGAAAC
    ACTGCTCTTC ACACTGCTGC TAAACATGGA CACGAGCTGC TCATCAGTGA ACTGCTGAAT
    AATGGTGCAG ACATAGCCAG GCGAGGCATT GATGGCATGA CTCCATTGCA CTTGGCGGTG
    TTGTACGGCT ATTCAGACTG TTGTCTTAAG CTACTTACCT CAGGGTTTGA TATAAACACA
    CTGGATGGCC TGAGCAGGAC GTGTTTACAT ATTGCTGCTT CAGGAGGGAA TGTCGAATGT
    CTTAAGCTGC TGTTGGACAG TGGTGCTGAT TGGAGTATAA AGGACAACAT AGGAAGGACT
    CCACTTCACG CATCGGCATA TTCAGGAAGT GTCTCAGCGC TGGTGTTAGC GTTGAGCTGC
    GCTTCAGATG TAGACTCAGT GGATGATGCC GGACGCTCCG CACTAATGTT CGCTGCTGAA
    AACGGACACA CGTCTGCTGT CGAAATCCTC CTTCATGAGG CCAGAGCGGA TCTGTCACTG
    CAAGACGTCA ACATGAACTC AGCTCTTCAT CTGGCCTGTA GAAGAGGTCA TGAAATGTGT
    GCTCTCCTCA TGCTGGCGGA GACTGACGAC ACGTCGCTCA TCAGCTCCAC AAACAGAGCG
    CTTCAGACGC CTCTGCATAT CGCAGCTAGA AATGGCCTTG TGACAGTGGT TGAGGTTCTG
    CTGACTCGAG GAGCAACAGT TCTGGCCGTA GATGAGGATG GACACACTCC AGCATTGGCT
    TGTGCACCAA ATAAAGAGGT GGCGAAGTGC CTGGCGCTCA TCATTTCCAC TATGAAGCCG
    TTCATCGGTT TGATGAAGAA CTGTGGCAGT GGTGTTTCCC ATCAGCCCCT GCGTAACGGC
    GTATCTCTAC ACCTGCTCAC CGAGGAGCCG CAGGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_693039.6
    CDS150..2048
    Translation

    Target ORF information:

    RefSeq Version XM_687947.10
    Organism Danio rerio(zebrafish)
    Definition Danio rerio ankyrin repeat domain 52b (ankrd52b), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_687947.10

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGGGACAC TCAACATTAG CGAGCAGTCT CTCCTGGTTC AGGCAGTGTT CAGCCAGAAT 
    GCTGAAATGC TGGAGTTTTT ATTGGAACAA AATGAAGACG TCAACGAATT GGACCAAGAG
    TGTCGCAGCG CTCTGCACGC CGCTGCGTTT GTGGGTGGTG CTCATATCAT GGACCTCCTC
    ATCAGTTCAG GTGCTAATGT TAATGCTAAA GATCAGGAGT GGTTGACTCC TCTGCATCGA
    GCAGCTGCTT CACAGAATGA AGTGTCTGTG GGCCTGCTGA TGGAAAAGGG AGCAGATGTG
    AACACACAGG ACAGATTCTG CCAGACGCCA TTACATGTAG CTGCAGCCAA CAGAGCCACT
    AAATGTGTGG ATGTTTTGCT TTTTCACGAG AGCAGTTTAA ATGCAGCGGA TTGTTTTGGG
    AGAACAGCTT TACATCACGC AGTGCACAGC GGGCACACAG AGACAGTGGC TGTGCTGCTG
    AACAAAGGGG CCAATGTGAG TGCCAGAGAC ATAAAGGACC GGCAGCCGCT CCATCTGGCC
    GCCTTTCTAG GGAACATGGA GGTCGTGAAG CTGTTGTTGG CGTTCAGAGC TGATGTGTCC
    TGCAGGGATA AACTGGCCTT CACTCCTTTA CATGCGGCAG CTCTTGGCGA ACACACTGAA
    GTGCTCAAAT ACCTGCTGAA ACATGGAGCA CAGATTGATG AAGCGGATGG TTCTGGAAAC
    ACTGCTCTTC ACATGGTGTG CAGTGCTGGT CTGGAGTCAG TAGTGTGTGA CCTGGTGAAC
    TGCGGTGCCG ATGTGAACCG TCCAAACCTG CGCGGCTCCA CACCTCTGCA TCTGTGTGCT
    GAGTCCCCCG ACGGAGCCCT GTGCCTGGAG CTGCTGGTCA ATAACGGAGC ACACGTTAAC
    ATTCCGAATA ATGAAGGGAA GAGTCCTTTG CACATGGCCG CCATTCATGG ACACTTCACC
    CGTTCCCAAA TACTCATTCA GAACGGCGCA GAAGTGGAGT GTGTGGATAA TTATGGAAAC
    ACTGCTCTTC ACACTGCTGC TAAACATGGA CACGAGCTGC TCATCAGTGA ACTGCTGAAT
    AATGGTGCAG ACATAGCCAG GCGAGGCATT GATGGCATGA CTCCATTGCA CTTGGCGGTG
    TTGTACGGCT ATTCAGACTG TTGTCTTAAG CTACTTACCT CAGGGTTTGA TATAAACACA
    CTGGATGGCC TGAGCAGGAC GTGTTTACAT ATTGCTGCTT CAGGAGGGAA TGTCGAATGT
    CTTAAGCTGC TGTTGGACAG TGGTGCTGAT TGGAGTATAA AGGACAACAT AGGAAGGACT
    CCACTTCACG CATCGGCATA TTCAGGAAGT GTCTCAGCGC TGGTGTTAGC GTTGAGCTGC
    GCTTCAGATG TAGACTCAGT GGATGATGCC GGACGCTCCG CACTAATGTT CGCTGCTGAA
    AACGGACACA CGTCTGCTGT CGAAATCCTC CTTCATGAGG CCAGAGCGGA TCTGTCACTG
    CAAGACGTCA ACATGAACTC AGCTCTTCAT CTGGCCTGTA GAAGAGGTCA TGAAATGTGT
    GCTCTCCTCA TGCTGGCGGA GACTGACGAC ACGTCGCTCA TCAGCTCCAC AAACAGAGCG
    CTTCAGACGC CTCTGCATAT CGCAGCTAGA AATGGCCTTG TGACAGTGGT TGAGGTTCTG
    CTGACTCGAG GAGCAACAGT TCTGGCCGTA GATGAGGATG GACACACTCC AGCATTGGCT
    TGTGCACCAA ATAAAGAGGT GGCGAAGTGC CTGGCGCTCA TCATTTCCAC TATGAAGCCG
    TTCATCGGTT TGATGAAGAA CTGTGGCAGT GGTGTTTCCC ATCAGCCCCT GCGTAACGGC
    GTATCTCTAC ACCTGCTCAC CGAGGAGCCG CAGGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa16662
    Clone ID Related Accession (Same CDS sequence) XM_687947.10 , XM_687947.9
    Accession Version XM_687947.9 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1899bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product serine/threonine-protein phosphatase 6 regulatory ankyrin repeat subunit C-like
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_687947.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGGGACAC TCAACATTAG CGAGCAGTCT CTCCTGGTTC AGGCAGTGTT CAGCCAGAAT 
    GCTGAAATGC TGGAGTTTTT ATTGGAACAA AATGAAGACG TCAACGAATT GGACCAAGAG
    TGTCGCAGCG CTCTGCACGC CGCTGCGTTT GTGGGTGGTG CTCATATCAT GGACCTCCTC
    ATCAGTTCAG GTGCTAATGT TAATGCTAAA GATCAGGAGT GGTTGACTCC TCTGCATCGA
    GCAGCTGCTT CACAGAATGA AGTGTCTGTG GGCCTGCTGA TGGAAAAGGG AGCAGATGTG
    AACACACAGG ACAGATTCTG CCAGACGCCA TTACATGTAG CTGCAGCCAA CAGAGCCACT
    AAATGTGTGG ATGTTTTGCT TTTTCACGAG AGCAGTTTAA ATGCAGCGGA TTGTTTTGGG
    AGAACAGCTT TACATCACGC AGTGCACAGC GGGCACACAG AGACAGTGGC TGTGCTGCTG
    AACAAAGGGG CCAATGTGAG TGCCAGAGAC ATAAAGGACC GGCAGCCGCT CCATCTGGCC
    GCCTTTCTAG GGAACATGGA GGTCGTGAAG CTGTTGTTGG CGTTCAGAGC TGATGTGTCC
    TGCAGGGATA AACTGGCCTT CACTCCTTTA CATGCGGCAG CTCTTGGCGA ACACACTGAA
    GTGCTCAAAT ACCTGCTGAA ACATGGAGCA CAGATTGATG AAGCGGATGG TTCTGGAAAC
    ACTGCTCTTC ACATGGTGTG CAGTGCTGGT CTGGAGTCAG TAGTGTGTGA CCTGGTGAAC
    TGCGGTGCCG ATGTGAACCG TCCAAACCTG CGCGGCTCCA CACCTCTGCA TCTGTGTGCT
    GAGTCCCCCG ACGGAGCCCT GTGCCTGGAG CTGCTGGTCA ATAACGGAGC ACACGTTAAC
    ATTCCGAATA ATGAAGGGAA GAGTCCTTTG CACATGGCCG CCATTCATGG ACACTTCACC
    CGTTCCCAAA TACTCATTCA GAACGGCGCA GAAGTGGAGT GTGTGGATAA TTATGGAAAC
    ACTGCTCTTC ACACTGCTGC TAAACATGGA CACGAGCTGC TCATCAGTGA ACTGCTGAAT
    AATGGTGCAG ACATAGCCAG GCGAGGCATT GATGGCATGA CTCCATTGCA CTTGGCGGTG
    TTGTACGGCT ATTCAGACTG TTGTCTTAAG CTACTTACCT CAGGGTTTGA TATAAACACA
    CTGGATGGCC TGAGCAGGAC GTGTTTACAT ATTGCTGCTT CAGGAGGGAA TGTCGAATGT
    CTTAAGCTGC TGTTGGACAG TGGTGCTGAT TGGAGTATAA AGGACAACAT AGGAAGGACT
    CCACTTCACG CATCGGCATA TTCAGGAAGT GTCTCAGCGC TGGTGTTAGC GTTGAGCTGC
    GCTTCAGATG TAGACTCAGT GGATGATGCC GGACGCTCCG CACTAATGTT CGCTGCTGAA
    AACGGACACA CGTCTGCTGT CGAAATCCTC CTTCATGAGG CCAGAGCGGA TCTGTCACTG
    CAAGACGTCA ACATGAACTC AGCTCTTCAT CTGGCCTGTA GAAGAGGTCA TGAAATGTGT
    GCTCTCCTCA TGCTGGCGGA GACTGACGAC ACGTCGCTCA TCAGCTCCAC AAACAGAGCG
    CTTCAGACGC CTCTGCATAT CGCAGCTAGA AATGGCCTTG TGACAGTGGT TGAGGTTCTG
    CTGACTCGAG GAGCAACAGT TCTGGCCGTA GATGAGGATG GACACACTCC AGCATTGGCT
    TGTGCACCAA ATAAAGAGGT GGCGAAGTGC CTGGCGCTCA TCATTTCCAC TATGAAGCCG
    TTCATCGGTT TGATGAAGAA CTGTGGCAGT GGTGTTTCCC ATCAGCCCCT GCGTAACGGC
    GTATCTCTAC ACCTGCTCAC CGAGGAGCCG CAGGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_693039.6
    CDS157..2055
    Translation

    Target ORF information:

    RefSeq Version XM_687947.9
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio ankyrin repeat domain 52b (ankrd52b), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_687947.9

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    ATGGGGACAC TCAACATTAG CGAGCAGTCT CTCCTGGTTC AGGCAGTGTT CAGCCAGAAT 
    GCTGAAATGC TGGAGTTTTT ATTGGAACAA AATGAAGACG TCAACGAATT GGACCAAGAG
    TGTCGCAGCG CTCTGCACGC CGCTGCGTTT GTGGGTGGTG CTCATATCAT GGACCTCCTC
    ATCAGTTCAG GTGCTAATGT TAATGCTAAA GATCAGGAGT GGTTGACTCC TCTGCATCGA
    GCAGCTGCTT CACAGAATGA AGTGTCTGTG GGCCTGCTGA TGGAAAAGGG AGCAGATGTG
    AACACACAGG ACAGATTCTG CCAGACGCCA TTACATGTAG CTGCAGCCAA CAGAGCCACT
    AAATGTGTGG ATGTTTTGCT TTTTCACGAG AGCAGTTTAA ATGCAGCGGA TTGTTTTGGG
    AGAACAGCTT TACATCACGC AGTGCACAGC GGGCACACAG AGACAGTGGC TGTGCTGCTG
    AACAAAGGGG CCAATGTGAG TGCCAGAGAC ATAAAGGACC GGCAGCCGCT CCATCTGGCC
    GCCTTTCTAG GGAACATGGA GGTCGTGAAG CTGTTGTTGG CGTTCAGAGC TGATGTGTCC
    TGCAGGGATA AACTGGCCTT CACTCCTTTA CATGCGGCAG CTCTTGGCGA ACACACTGAA
    GTGCTCAAAT ACCTGCTGAA ACATGGAGCA CAGATTGATG AAGCGGATGG TTCTGGAAAC
    ACTGCTCTTC ACATGGTGTG CAGTGCTGGT CTGGAGTCAG TAGTGTGTGA CCTGGTGAAC
    TGCGGTGCCG ATGTGAACCG TCCAAACCTG CGCGGCTCCA CACCTCTGCA TCTGTGTGCT
    GAGTCCCCCG ACGGAGCCCT GTGCCTGGAG CTGCTGGTCA ATAACGGAGC ACACGTTAAC
    ATTCCGAATA ATGAAGGGAA GAGTCCTTTG CACATGGCCG CCATTCATGG ACACTTCACC
    CGTTCCCAAA TACTCATTCA GAACGGCGCA GAAGTGGAGT GTGTGGATAA TTATGGAAAC
    ACTGCTCTTC ACACTGCTGC TAAACATGGA CACGAGCTGC TCATCAGTGA ACTGCTGAAT
    AATGGTGCAG ACATAGCCAG GCGAGGCATT GATGGCATGA CTCCATTGCA CTTGGCGGTG
    TTGTACGGCT ATTCAGACTG TTGTCTTAAG CTACTTACCT CAGGGTTTGA TATAAACACA
    CTGGATGGCC TGAGCAGGAC GTGTTTACAT ATTGCTGCTT CAGGAGGGAA TGTCGAATGT
    CTTAAGCTGC TGTTGGACAG TGGTGCTGAT TGGAGTATAA AGGACAACAT AGGAAGGACT
    CCACTTCACG CATCGGCATA TTCAGGAAGT GTCTCAGCGC TGGTGTTAGC GTTGAGCTGC
    GCTTCAGATG TAGACTCAGT GGATGATGCC GGACGCTCCG CACTAATGTT CGCTGCTGAA
    AACGGACACA CGTCTGCTGT CGAAATCCTC CTTCATGAGG CCAGAGCGGA TCTGTCACTG
    CAAGACGTCA ACATGAACTC AGCTCTTCAT CTGGCCTGTA GAAGAGGTCA TGAAATGTGT
    GCTCTCCTCA TGCTGGCGGA GACTGACGAC ACGTCGCTCA TCAGCTCCAC AAACAGAGCG
    CTTCAGACGC CTCTGCATAT CGCAGCTAGA AATGGCCTTG TGACAGTGGT TGAGGTTCTG
    CTGACTCGAG GAGCAACAGT TCTGGCCGTA GATGAGGATG GACACACTCC AGCATTGGCT
    TGTGCACCAA ATAAAGAGGT GGCGAAGTGC CTGGCGCTCA TCATTTCCAC TATGAAGCCG
    TTCATCGGTT TGATGAAGAA CTGTGGCAGT GGTGTTTCCC ATCAGCCCCT GCGTAACGGC
    GTATCTCTAC ACCTGCTCAC CGAGGAGCCG CAGGGCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.