ush1c cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ush1c
Entrez Gene ID	564412
Full Name	Usher syndrome 1C
Synonyms	zgc:136806
General protein information	Preferred Names Usher syndrome 1C Names harmonin Usher syndrome 1C (autosomal recessive, severe) harmonin pseudogene
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009298037.2	XP_009296312.1	harmonin isoform X1
XM_009298038.2	XP_009296313.1	harmonin isoform X1
XM_017354085.1	XP_017209574.1	harmonin isoform X2
XM_017354086.1	XP_017209575.1	harmonin isoform X3
XM_009298037.3	XP_009296312.1	harmonin isoform X1
NM_001039929.1	NP_001035018.1	harmonin
XM_009298038.3	XP_009296313.1	harmonin isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	USH1C	NP_710142.1
Bos taurus (cattle)	USH1C	NP_001030459.1
Danio rerio (zebrafish)	ush1c	NP_001035018.1
Xenopus tropicalis (tropical clawed frog)	ush1c	NP_001096522.1
Pan troglodytes (chimpanzee)	USH1C	XP_003951893.1
Canis lupus familiaris (dog)	USH1C	XP_005633777.1
Mus musculus (house mouse)	Ush1c	NP_710143.2
Gallus gallus (chicken)	USH1C	XP_421006.4

Gene Ontology
Bibliography

Related articles in PubMed

High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9.
Varshney GK, Pei W, LaFave MC, Idol J, Xu L, Gallardo V, Carrington B, Bishop K, Jones M, Li M, Harper U, Huang SC, Prakash A, Chen W, Sood R, Ledin J, Burgess SM
Genome research25(7)1030-42(2015 Jul)

Complexes of Usher proteins preassemble at the endoplasmic reticulum and are required for trafficking and ER homeostasis.
Blanco-S?nchez B, Cl?ment A, Fierro J Jr, Washbourne P, Westerfield M
Disease models & mechanisms7(5)547-59(2014 May)

Harmonin (Ush1c) is required in zebrafish M?ller glial cells for photoreceptor synaptic development and function.
Phillips JB, Blanco-Sanchez B, Lentz JJ, Tallafuss A, Khanobdee K, Sampath S, Jacobs ZG, Han PF, Mishra M, Titus TA, Williams DS, Keats BJ, Washbourne P, Westerfield M
Disease models & mechanisms4(6)786-800(2011 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

This study shows that visual defects associated with loss of ush1c function in zebrafish can be detected from the onset of vision.
Title: Harmonin (Ush1c) is required in zebrafish M?ller glial cells for photoreceptor synaptic development and function.

The following ush1c gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ush1c cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa03207	NM_001039929.1 Latest version!	Danio rerio Usher syndrome 1C (ush1c), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa44328	XM_009298038.3 Latest version!	Danio rerio Usher syndrome 1C (ush1c), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa44328	XM_009298037.3 Latest version!	Danio rerio Usher syndrome 1C (ush1c), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa57802	XM_017354085.1 Latest version!	Danio rerio Usher syndrome 1C (ush1c), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
ODa57803	XM_017354086.1 Latest version!	Danio rerio Usher syndrome 1C (ush1c), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$258.30 $369.00
View Old Accession Versions >>
ODa44328	XM_009298037.2	Danio rerio Usher syndrome 1C (ush1c), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa44328	XM_009298038.2	Danio rerio Usher syndrome 1C (ush1c), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ush1c ( NM_001039929.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001035018.1 Danio rerio Usher syndrome 1C (ush1c), mRNA.

Price & Availability↑

CloneID	ODa03207
Clone ID Related Accession (Same CDS sequence)	NM_001039929.1
Accession Version	NM_001039929.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-15
Organism	Danio rerio(zebrafish)
Product	harmonin
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC114278.1. On Mar 22, 2006 this sequence version replaced XM_687750.1. ##Evidence-Data-START## Transcript exon combination :: BC114278.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGGAAAGAA AAGTGGCCAG GGAATTTCGG CATAAGGTGG AGTTGCTTAT TGATAATGAA 
GCAGAGAAGG ACTACCTTTA TGATGTTCTT CGCATGTACC ATCAATCCAT GGACCTGCCT 
GTCCTTGTTG GTGACCTGAA GCTGGTAATA AATGAGCCCA AACGCCTTCC TTTGTTTGAT 
GCCATCAGAC CCTTGATTCC TCTGAAGCAC CAAGTGCAGT ATGACCAGCT CACGCCCAAG 
AGATCCAGGA AGTTGAAGGA AGTTCGTCTG GATCGCACAC ACCCCGAGGG TCTGGGTTTG 
AGTGTTCGAG GAGGACTGGA GTTTGGCTCT GGCCTCTTTA TATCGCAGAT AGTCAAAGAT 
GGACAGGCTG GAAATGTCGG CCTGCAGGTC GGTGATGAGA TTGTGCGCAT CAACGGCTAC 
TCCATCTCTT CCTGCATTCA CGAGGAGGTC ATCAACCTCA TCAAGACCAA GAAGACTGTG 
TCTTTGAAAG TCCGCCATGT GGGAATGATT CCTGTCAAGA GCTCCTCCGA TGAGCCTCTG 
AAGTGGCAGT TTGTGGATCA GTTTGTTTCG GAGTCAGGGG AGAAGAGTAA CAGTATTGCT 
GGCTTGGCTT CAATTGGAGG GAATGAAATT AAGGAAAAGA AAGTGTTCCT TAGTTTGGTG 
GGCACCAAAG GCATGGGAAT CAGCATATCA AGTGGACCAA CAAAGAAACC AGGCATCTAC 
GTGAGCAATG TGAAGCCGGG TTCACTCTCA GCTGAAGTGG GCTTACAGGT TGGCGACCAG 
ATTGTGGAGG TCAATGGGGT GGAATTCACC AACCTGAGTC ATCAAGAGGC AGTGCGGGTG 
TTAAAGAGCA GCAGGAGTCT AACCATCACC GTACTCACTG GGGCTGGAAG TGAGCTGTTT 
ATGACGGATG AGGAGCGGTT GGCGGTTGAA GCTCGACATG AGCTGGACAG ACAGGAGTTA 
ATGCATCAGA AGAAAGTGGC ATTAGAGACC AACAAAATTG TCAAAGAACA ACAGGAGAAA 
GAACGCCAGA GGAAGATGGA GATATCTCAG AAGGCTGCAG AGGAAGAAGA GCGCTATCGC 
AAAGAGATGG AAAGAATTGA AGCTGCAGAG AAGAAACACC ACAAAGAGTG GGAAGATGAT 
TGGGGCTCCA AGGAAAAAAA AAAATCTGTT TCCCCGGCCG CTTCTCCATC CCCTCCACCA 
CCCACACGTC TTGCACCTTC ACCCAAACCT AAAACCTCCA CTGCTGATTC CTACGAAGAA 
GAGACCGGAG AAGGGTTTCA GAAATACGTG GATGATTTTG ATCCACATTC CATGTTCACA 
GCCGAACAGA TAGCAGGCAG AGATGTGCGA CTTTTAAGAA TTAAGAAGGA AGGAAAGCTT 
GATCTGGCCA TTGAGGGAGG AATCGATTCC CCATTGGGAA AGATTGTGGT GTCGTCTATC 
TATGAAGGAG GTGCAGCAGA TAAACATGGT GGGGTTGTGT CAGGTGATGA GATCATGGCA 
GTGAATGGAA AGATTTTGAC AGATGTGACT CTAGCTGAAG GACAGACCTC AATGGCTCAG 
GCCTGGAACA GTGGAGGGGA CTGGATTGAC CTGGTCATCG CAGTGTCTCC ACCAAAGGAA 
TATGAAGATG AGGTGACATT CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001035018.1
CDS	65..1711
Translation	MERKVAREFRHKVELLIDNEAEKDYLYDVLRMYHQSMDLPVLVGDLKLVINEPKRLPLFDAIRPLIPLKHQVQYDQLTPKRSRKLKEVRLDRTHPEGLGLSVRGGLEFGSGLFISQIVKDGQAGNVGLQVGDEIVRINGYSISSCIHEEVINLIKTKKTVSLKVRHVGMIPVKSSSDEPLKWQFVDQFVSESGEKSNSIAGLASIGGNEIKEKKVFLSLVGTKGMGISISSGPTKKPGIYVSNVKPGSLSAEVGLQVGDQIVEVNGVEFTNLSHQEAVRVLKSSRSLTITVLTGAGSELFMTDEERLAVEARHELDRQELMHQKKVALETNKIVKEQQEKERQRKMEISQKAAEEEERYRKEMERIEAAEKKHHKEWEDDWGSKEKKKSVSPAASPSPPPPTRLAPSPKPKTSTADSYEEETGEGFQKYVDDFDPHSMFTAEQIAGRDVRLLRIKKEGKLDLAIEGGIDSPLGKIVVSSIYEGGAADKHGGVVSGDEIMAVNGKILTDVTLAEGQTSMAQAWNSGGDWIDLVIAVSPPKEYEDEVTFF

Target ORF information:

RefSeq Version	NM_001039929.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Usher syndrome 1C (ush1c), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001039929.1

ORF Insert Sequence:

ATGGAAAGAA AAGTGGCCAG GGAATTTCGG CATAAGGTGG AGTTGCTTAT TGATAATGAA 
GCAGAGAAGG ACTACCTTTA TGATGTTCTT CGCATGTACC ATCAATCCAT GGACCTGCCT 
GTCCTTGTTG GTGACCTGAA GCTGGTAATA AATGAGCCCA AACGCCTTCC TTTGTTTGAT 
GCCATCAGAC CCTTGATTCC TCTGAAGCAC CAAGTGCAGT ATGACCAGCT CACGCCCAAG 
AGATCCAGGA AGTTGAAGGA AGTTCGTCTG GATCGCACAC ACCCCGAGGG TCTGGGTTTG 
AGTGTTCGAG GAGGACTGGA GTTTGGCTCT GGCCTCTTTA TATCGCAGAT AGTCAAAGAT 
GGACAGGCTG GAAATGTCGG CCTGCAGGTC GGTGATGAGA TTGTGCGCAT CAACGGCTAC 
TCCATCTCTT CCTGCATTCA CGAGGAGGTC ATCAACCTCA TCAAGACCAA GAAGACTGTG 
TCTTTGAAAG TCCGCCATGT GGGAATGATT CCTGTCAAGA GCTCCTCCGA TGAGCCTCTG 
AAGTGGCAGT TTGTGGATCA GTTTGTTTCG GAGTCAGGGG AGAAGAGTAA CAGTATTGCT 
GGCTTGGCTT CAATTGGAGG GAATGAAATT AAGGAAAAGA AAGTGTTCCT TAGTTTGGTG 
GGCACCAAAG GCATGGGAAT CAGCATATCA AGTGGACCAA CAAAGAAACC AGGCATCTAC 
GTGAGCAATG TGAAGCCGGG TTCACTCTCA GCTGAAGTGG GCTTACAGGT TGGCGACCAG 
ATTGTGGAGG TCAATGGGGT GGAATTCACC AACCTGAGTC ATCAAGAGGC AGTGCGGGTG 
TTAAAGAGCA GCAGGAGTCT AACCATCACC GTACTCACTG GGGCTGGAAG TGAGCTGTTT 
ATGACGGATG AGGAGCGGTT GGCGGTTGAA GCTCGACATG AGCTGGACAG ACAGGAGTTA 
ATGCATCAGA AGAAAGTGGC ATTAGAGACC AACAAAATTG TCAAAGAACA ACAGGAGAAA 
GAACGCCAGA GGAAGATGGA GATATCTCAG AAGGCTGCAG AGGAAGAAGA GCGCTATCGC 
AAAGAGATGG AAAGAATTGA AGCTGCAGAG AAGAAACACC ACAAAGAGTG GGAAGATGAT 
TGGGGCTCCA AGGAAAAAAA AAAATCTGTT TCCCCGGCCG CTTCTCCATC CCCTCCACCA 
CCCACACGTC TTGCACCTTC ACCCAAACCT AAAACCTCCA CTGCTGATTC CTACGAAGAA 
GAGACCGGAG AAGGGTTTCA GAAATACGTG GATGATTTTG ATCCACATTC CATGTTCACA 
GCCGAACAGA TAGCAGGCAG AGATGTGCGA CTTTTAAGAA TTAAGAAGGA AGGAAAGCTT 
GATCTGGCCA TTGAGGGAGG AATCGATTCC CCATTGGGAA AGATTGTGGT GTCGTCTATC 
TATGAAGGAG GTGCAGCAGA TAAACATGGT GGGGTTGTGT CAGGTGATGA GATCATGGCA 
GTGAATGGAA AGATTTTGAC AGATGTGACT CTAGCTGAAG GACAGACCTC AATGGCTCAG 
GCCTGGAACA GTGGAGGGGA CTGGATTGAC CTGGTCATCG CAGTGTCTCC ACCAAAGGAA 
TATGAAGATG AGGTGACATT CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ush1c ( XM_009298037.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296312.1 Danio rerio Usher syndrome 1C (ush1c), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa44328
Clone ID Related Accession (Same CDS sequence)	XM_009298037.2 , XM_009298038.2 , XM_009298037.3 , XM_009298038.3
Accession Version	XM_009298037.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	harmonin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298037.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGAA AAGTGGCCAG GGAATTTCGG CATAAGGTGG AGTTGCTTAT TGATAATGAA 
GCAGAGAAGG ACTACCTTTA TGATGTTCTT CGCATGTACC ATCAATCCAT GGACCTGCCT 
GTCCTTGTTG GTGACCTGAA GCTGGTAATA AATGAGCCCA AACGCCTTCC TTTGTTTGAT 
GCCATCAGAC CCTTGATTCC TCTGAAGCAC CAAGTGCAGT ATGACCAGCT CACGCCCAAG 
AGATCCAGGA AGTTGAAGGA AGTTCGTCTG GATCGCACAC ACCCCGAGGG TCTGGGTTTG 
AGTGTTCGAG GAGGACTGGA GTTTGGCTCT GGCCTCTTTA TATCGCAGAT AGTCAAAGAT 
GGACAGGCTG GAAATGTCGG CCTGCAGGTC GGTGATGAGA TTGTGCGCAT CAACGGCTAC 
TCCATCTCTT CCTGCATTCA CGAGGAGGTC ATCAACCTCA TCAAGACCAA GAAGACTGTG 
TCTTTGAAAG TCCGCCATGT GGGAATGATT CCTGTCAAGA GCTCCTCCGA TGAGCCTCTG 
AAGTGGCAGT TTGTGGATCA GTTTGTTTCG GAGTCAGGGG AGAAGAGTAA CAGTATTGCT 
GGCTTGGCTT CAATTGGAGG GAATGAAATT AAGGAAAAGA AAGTGTTCCT TAGTTTGGTG 
GGCACCAAAG GCATGGGAAT CAGCATATCA AGTGGACCAA CAAAGAAACC AGGCATCTAC 
GTGAGCAATG TGAAGCCGGG TTCACTCTCA GCTGAAGTGG GCTTACAGGT TGGCGACCAG 
ATTGTGGAGG TCAATGGGGT GGAATTCACC AACCTGAGTC ATCAAGAGGC AGTGCGGGTG 
TTAAAGAGCA GCAGGAGTCT AACCATCACC GTACTCACTG GGGCTGGAAG TGAGCTGTTT 
ATGACGGATG AGGAGCGGTT GGCGGTTGAA GCTCGACATG AGCTGGACAG ACAGGAGTTA 
ATGCATCAGA AGAAAGTGGC ATTAGAGACC AACAAAATTG TCAAAGAACA ACAGGAGAAA 
GAACGCCAGA GGAAGATGGA GATATCTCAG AAGGCTGCAG AGGAAGAAGA GCGCTATCGC 
AAAGAGATGG AAAGAATTGA AGCTGCAGAG AAGAAACACC ACAAAGAGTG GGAAGATGAT 
TGGGGCTCCA AGGAAAAAAA AAAATCTGTT TCCCCGGCCG CTTCTCCATC CCCTCCACCA 
CCCACACGTC TTGCACCTTC ACCCAAACCT AAAACCTCCA CTGCTGATTC CTACGAAGAA 
GAGACCGGAG AAGGGTTTCA GAAATACGTG GATGATTTTG ATCCACATTC CATGTTCACA 
GCCGAACAGA TAGCAGGCAG AGATGTGCGA CTTTTAAGAA TTAAGAAGGA AGGAAAGCTT 
GATCTGGCCA TTGAGGGAGG AATCGATTCC CCATTGGGAA AGATTGTGGT GTCGTCTATC 
TATGAAGGAG GTGCAGCAGA TAAACATGGT GGGGTTGTGT CAGGTGATGA GATCATGGCA 
GTGAATGGAA AGATTTTGAC AGATGTGACT CTAGCTGAAG GACAGACCTC AATGGCTCAG 
GCCTGGAACA GTGGAGGGGA CTGGATTGAC CTGGTCATCG CAGTGTCTCC ACCAAAGGAA 
TATGAAGATG AGGTCCCTAA AGCAGCATCA GTCAGACCCA CTGCAAAGAA AACGGTTTTT 
CAAGGCAGTG CCCCTGTGTA CAGACAGGGA TACGTCCTTC AGATGAAGAC CCCACCATCC 
CCCAACTTCA TCAGCCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296312.1
CDS	110..1870
Translation	MERKVAREFRHKVELLIDNEAEKDYLYDVLRMYHQSMDLPVLVGDLKLVINEPKRLPLFDAIRPLIPLKHQVQYDQLTPKRSRKLKEVRLDRTHPEGLGLSVRGGLEFGSGLFISQIVKDGQAGNVGLQVGDEIVRINGYSISSCIHEEVINLIKTKKTVSLKVRHVGMIPVKSSSDEPLKWQFVDQFVSESGEKSNSIAGLASIGGNEIKEKKVFLSLVGTKGMGISISSGPTKKPGIYVSNVKPGSLSAEVGLQVGDQIVEVNGVEFTNLSHQEAVRVLKSSRSLTITVLTGAGSELFMTDEERLAVEARHELDRQELMHQKKVALETNKIVKEQQEKERQRKMEISQKAAEEEERYRKEMERIEAAEKKHHKEWEDDWGSKEKKKSVSPAASPSPPPPTRLAPSPKPKTSTADSYEEETGEGFQKYVDDFDPHSMFTAEQIAGRDVRLLRIKKEGKLDLAIEGGIDSPLGKIVVSSIYEGGAADKHGGVVSGDEIMAVNGKILTDVTLAEGQTSMAQAWNSGGDWIDLVIAVSPPKEYEDEVPKAASVRPTAKKTVFQGSAPVYRQGYVLQMKTPPSPNFISH

Target ORF information:

RefSeq Version	XM_009298037.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Usher syndrome 1C (ush1c), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298037.2

ORF Insert Sequence:

ATGGAAAGAA AAGTGGCCAG GGAATTTCGG CATAAGGTGG AGTTGCTTAT TGATAATGAA 
GCAGAGAAGG ACTACCTTTA TGATGTTCTT CGCATGTACC ATCAATCCAT GGACCTGCCT 
GTCCTTGTTG GTGACCTGAA GCTGGTAATA AATGAGCCCA AACGCCTTCC TTTGTTTGAT 
GCCATCAGAC CCTTGATTCC TCTGAAGCAC CAAGTGCAGT ATGACCAGCT CACGCCCAAG 
AGATCCAGGA AGTTGAAGGA AGTTCGTCTG GATCGCACAC ACCCCGAGGG TCTGGGTTTG 
AGTGTTCGAG GAGGACTGGA GTTTGGCTCT GGCCTCTTTA TATCGCAGAT AGTCAAAGAT 
GGACAGGCTG GAAATGTCGG CCTGCAGGTC GGTGATGAGA TTGTGCGCAT CAACGGCTAC 
TCCATCTCTT CCTGCATTCA CGAGGAGGTC ATCAACCTCA TCAAGACCAA GAAGACTGTG 
TCTTTGAAAG TCCGCCATGT GGGAATGATT CCTGTCAAGA GCTCCTCCGA TGAGCCTCTG 
AAGTGGCAGT TTGTGGATCA GTTTGTTTCG GAGTCAGGGG AGAAGAGTAA CAGTATTGCT 
GGCTTGGCTT CAATTGGAGG GAATGAAATT AAGGAAAAGA AAGTGTTCCT TAGTTTGGTG 
GGCACCAAAG GCATGGGAAT CAGCATATCA AGTGGACCAA CAAAGAAACC AGGCATCTAC 
GTGAGCAATG TGAAGCCGGG TTCACTCTCA GCTGAAGTGG GCTTACAGGT TGGCGACCAG 
ATTGTGGAGG TCAATGGGGT GGAATTCACC AACCTGAGTC ATCAAGAGGC AGTGCGGGTG 
TTAAAGAGCA GCAGGAGTCT AACCATCACC GTACTCACTG GGGCTGGAAG TGAGCTGTTT 
ATGACGGATG AGGAGCGGTT GGCGGTTGAA GCTCGACATG AGCTGGACAG ACAGGAGTTA 
ATGCATCAGA AGAAAGTGGC ATTAGAGACC AACAAAATTG TCAAAGAACA ACAGGAGAAA 
GAACGCCAGA GGAAGATGGA GATATCTCAG AAGGCTGCAG AGGAAGAAGA GCGCTATCGC 
AAAGAGATGG AAAGAATTGA AGCTGCAGAG AAGAAACACC ACAAAGAGTG GGAAGATGAT 
TGGGGCTCCA AGGAAAAAAA AAAATCTGTT TCCCCGGCCG CTTCTCCATC CCCTCCACCA 
CCCACACGTC TTGCACCTTC ACCCAAACCT AAAACCTCCA CTGCTGATTC CTACGAAGAA 
GAGACCGGAG AAGGGTTTCA GAAATACGTG GATGATTTTG ATCCACATTC CATGTTCACA 
GCCGAACAGA TAGCAGGCAG AGATGTGCGA CTTTTAAGAA TTAAGAAGGA AGGAAAGCTT 
GATCTGGCCA TTGAGGGAGG AATCGATTCC CCATTGGGAA AGATTGTGGT GTCGTCTATC 
TATGAAGGAG GTGCAGCAGA TAAACATGGT GGGGTTGTGT CAGGTGATGA GATCATGGCA 
GTGAATGGAA AGATTTTGAC AGATGTGACT CTAGCTGAAG GACAGACCTC AATGGCTCAG 
GCCTGGAACA GTGGAGGGGA CTGGATTGAC CTGGTCATCG CAGTGTCTCC ACCAAAGGAA 
TATGAAGATG AGGTCCCTAA AGCAGCATCA GTCAGACCCA CTGCAAAGAA AACGGTTTTT 
CAAGGCAGTG CCCCTGTGTA CAGACAGGGA TACGTCCTTC AGATGAAGAC CCCACCATCC 
CCCAACTTCA TCAGCCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ush1c ( XM_009298038.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296313.1 Danio rerio Usher syndrome 1C (ush1c), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa44328
Clone ID Related Accession (Same CDS sequence)	XM_009298037.2 , XM_009298038.2 , XM_009298037.3 , XM_009298038.3
Accession Version	XM_009298038.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	harmonin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298038.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGAA AAGTGGCCAG GGAATTTCGG CATAAGGTGG AGTTGCTTAT TGATAATGAA 
GCAGAGAAGG ACTACCTTTA TGATGTTCTT CGCATGTACC ATCAATCCAT GGACCTGCCT 
GTCCTTGTTG GTGACCTGAA GCTGGTAATA AATGAGCCCA AACGCCTTCC TTTGTTTGAT 
GCCATCAGAC CCTTGATTCC TCTGAAGCAC CAAGTGCAGT ATGACCAGCT CACGCCCAAG 
AGATCCAGGA AGTTGAAGGA AGTTCGTCTG GATCGCACAC ACCCCGAGGG TCTGGGTTTG 
AGTGTTCGAG GAGGACTGGA GTTTGGCTCT GGCCTCTTTA TATCGCAGAT AGTCAAAGAT 
GGACAGGCTG GAAATGTCGG CCTGCAGGTC GGTGATGAGA TTGTGCGCAT CAACGGCTAC 
TCCATCTCTT CCTGCATTCA CGAGGAGGTC ATCAACCTCA TCAAGACCAA GAAGACTGTG 
TCTTTGAAAG TCCGCCATGT GGGAATGATT CCTGTCAAGA GCTCCTCCGA TGAGCCTCTG 
AAGTGGCAGT TTGTGGATCA GTTTGTTTCG GAGTCAGGGG AGAAGAGTAA CAGTATTGCT 
GGCTTGGCTT CAATTGGAGG GAATGAAATT AAGGAAAAGA AAGTGTTCCT TAGTTTGGTG 
GGCACCAAAG GCATGGGAAT CAGCATATCA AGTGGACCAA CAAAGAAACC AGGCATCTAC 
GTGAGCAATG TGAAGCCGGG TTCACTCTCA GCTGAAGTGG GCTTACAGGT TGGCGACCAG 
ATTGTGGAGG TCAATGGGGT GGAATTCACC AACCTGAGTC ATCAAGAGGC AGTGCGGGTG 
TTAAAGAGCA GCAGGAGTCT AACCATCACC GTACTCACTG GGGCTGGAAG TGAGCTGTTT 
ATGACGGATG AGGAGCGGTT GGCGGTTGAA GCTCGACATG AGCTGGACAG ACAGGAGTTA 
ATGCATCAGA AGAAAGTGGC ATTAGAGACC AACAAAATTG TCAAAGAACA ACAGGAGAAA 
GAACGCCAGA GGAAGATGGA GATATCTCAG AAGGCTGCAG AGGAAGAAGA GCGCTATCGC 
AAAGAGATGG AAAGAATTGA AGCTGCAGAG AAGAAACACC ACAAAGAGTG GGAAGATGAT 
TGGGGCTCCA AGGAAAAAAA AAAATCTGTT TCCCCGGCCG CTTCTCCATC CCCTCCACCA 
CCCACACGTC TTGCACCTTC ACCCAAACCT AAAACCTCCA CTGCTGATTC CTACGAAGAA 
GAGACCGGAG AAGGGTTTCA GAAATACGTG GATGATTTTG ATCCACATTC CATGTTCACA 
GCCGAACAGA TAGCAGGCAG AGATGTGCGA CTTTTAAGAA TTAAGAAGGA AGGAAAGCTT 
GATCTGGCCA TTGAGGGAGG AATCGATTCC CCATTGGGAA AGATTGTGGT GTCGTCTATC 
TATGAAGGAG GTGCAGCAGA TAAACATGGT GGGGTTGTGT CAGGTGATGA GATCATGGCA 
GTGAATGGAA AGATTTTGAC AGATGTGACT CTAGCTGAAG GACAGACCTC AATGGCTCAG 
GCCTGGAACA GTGGAGGGGA CTGGATTGAC CTGGTCATCG CAGTGTCTCC ACCAAAGGAA 
TATGAAGATG AGGTCCCTAA AGCAGCATCA GTCAGACCCA CTGCAAAGAA AACGGTTTTT 
CAAGGCAGTG CCCCTGTGTA CAGACAGGGA TACGTCCTTC AGATGAAGAC CCCACCATCC 
CCCAACTTCA TCAGCCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296313.1
CDS	110..1870
Translation	MERKVAREFRHKVELLIDNEAEKDYLYDVLRMYHQSMDLPVLVGDLKLVINEPKRLPLFDAIRPLIPLKHQVQYDQLTPKRSRKLKEVRLDRTHPEGLGLSVRGGLEFGSGLFISQIVKDGQAGNVGLQVGDEIVRINGYSISSCIHEEVINLIKTKKTVSLKVRHVGMIPVKSSSDEPLKWQFVDQFVSESGEKSNSIAGLASIGGNEIKEKKVFLSLVGTKGMGISISSGPTKKPGIYVSNVKPGSLSAEVGLQVGDQIVEVNGVEFTNLSHQEAVRVLKSSRSLTITVLTGAGSELFMTDEERLAVEARHELDRQELMHQKKVALETNKIVKEQQEKERQRKMEISQKAAEEEERYRKEMERIEAAEKKHHKEWEDDWGSKEKKKSVSPAASPSPPPPTRLAPSPKPKTSTADSYEEETGEGFQKYVDDFDPHSMFTAEQIAGRDVRLLRIKKEGKLDLAIEGGIDSPLGKIVVSSIYEGGAADKHGGVVSGDEIMAVNGKILTDVTLAEGQTSMAQAWNSGGDWIDLVIAVSPPKEYEDEVPKAASVRPTAKKTVFQGSAPVYRQGYVLQMKTPPSPNFISH

Target ORF information:

RefSeq Version	XM_009298038.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Usher syndrome 1C (ush1c), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298038.2

ORF Insert Sequence:

ATGGAAAGAA AAGTGGCCAG GGAATTTCGG CATAAGGTGG AGTTGCTTAT TGATAATGAA 
GCAGAGAAGG ACTACCTTTA TGATGTTCTT CGCATGTACC ATCAATCCAT GGACCTGCCT 
GTCCTTGTTG GTGACCTGAA GCTGGTAATA AATGAGCCCA AACGCCTTCC TTTGTTTGAT 
GCCATCAGAC CCTTGATTCC TCTGAAGCAC CAAGTGCAGT ATGACCAGCT CACGCCCAAG 
AGATCCAGGA AGTTGAAGGA AGTTCGTCTG GATCGCACAC ACCCCGAGGG TCTGGGTTTG 
AGTGTTCGAG GAGGACTGGA GTTTGGCTCT GGCCTCTTTA TATCGCAGAT AGTCAAAGAT 
GGACAGGCTG GAAATGTCGG CCTGCAGGTC GGTGATGAGA TTGTGCGCAT CAACGGCTAC 
TCCATCTCTT CCTGCATTCA CGAGGAGGTC ATCAACCTCA TCAAGACCAA GAAGACTGTG 
TCTTTGAAAG TCCGCCATGT GGGAATGATT CCTGTCAAGA GCTCCTCCGA TGAGCCTCTG 
AAGTGGCAGT TTGTGGATCA GTTTGTTTCG GAGTCAGGGG AGAAGAGTAA CAGTATTGCT 
GGCTTGGCTT CAATTGGAGG GAATGAAATT AAGGAAAAGA AAGTGTTCCT TAGTTTGGTG 
GGCACCAAAG GCATGGGAAT CAGCATATCA AGTGGACCAA CAAAGAAACC AGGCATCTAC 
GTGAGCAATG TGAAGCCGGG TTCACTCTCA GCTGAAGTGG GCTTACAGGT TGGCGACCAG 
ATTGTGGAGG TCAATGGGGT GGAATTCACC AACCTGAGTC ATCAAGAGGC AGTGCGGGTG 
TTAAAGAGCA GCAGGAGTCT AACCATCACC GTACTCACTG GGGCTGGAAG TGAGCTGTTT 
ATGACGGATG AGGAGCGGTT GGCGGTTGAA GCTCGACATG AGCTGGACAG ACAGGAGTTA 
ATGCATCAGA AGAAAGTGGC ATTAGAGACC AACAAAATTG TCAAAGAACA ACAGGAGAAA 
GAACGCCAGA GGAAGATGGA GATATCTCAG AAGGCTGCAG AGGAAGAAGA GCGCTATCGC 
AAAGAGATGG AAAGAATTGA AGCTGCAGAG AAGAAACACC ACAAAGAGTG GGAAGATGAT 
TGGGGCTCCA AGGAAAAAAA AAAATCTGTT TCCCCGGCCG CTTCTCCATC CCCTCCACCA 
CCCACACGTC TTGCACCTTC ACCCAAACCT AAAACCTCCA CTGCTGATTC CTACGAAGAA 
GAGACCGGAG AAGGGTTTCA GAAATACGTG GATGATTTTG ATCCACATTC CATGTTCACA 
GCCGAACAGA TAGCAGGCAG AGATGTGCGA CTTTTAAGAA TTAAGAAGGA AGGAAAGCTT 
GATCTGGCCA TTGAGGGAGG AATCGATTCC CCATTGGGAA AGATTGTGGT GTCGTCTATC 
TATGAAGGAG GTGCAGCAGA TAAACATGGT GGGGTTGTGT CAGGTGATGA GATCATGGCA 
GTGAATGGAA AGATTTTGAC AGATGTGACT CTAGCTGAAG GACAGACCTC AATGGCTCAG 
GCCTGGAACA GTGGAGGGGA CTGGATTGAC CTGGTCATCG CAGTGTCTCC ACCAAAGGAA 
TATGAAGATG AGGTCCCTAA AGCAGCATCA GTCAGACCCA CTGCAAAGAA AACGGTTTTT 
CAAGGCAGTG CCCCTGTGTA CAGACAGGGA TACGTCCTTC AGATGAAGAC CCCACCATCC 
CCCAACTTCA TCAGCCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ush1c ( XM_009298037.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296312.1 Danio rerio Usher syndrome 1C (ush1c), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa44328
Clone ID Related Accession (Same CDS sequence)	XM_009298037.2 , XM_009298038.2 , XM_009298037.3 , XM_009298038.3
Accession Version	XM_009298037.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	harmonin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298037.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGAA AAGTGGCCAG GGAATTTCGG CATAAGGTGG AGTTGCTTAT TGATAATGAA 
GCAGAGAAGG ACTACCTTTA TGATGTTCTT CGCATGTACC ATCAATCCAT GGACCTGCCT 
GTCCTTGTTG GTGACCTGAA GCTGGTAATA AATGAGCCCA AACGCCTTCC TTTGTTTGAT 
GCCATCAGAC CCTTGATTCC TCTGAAGCAC CAAGTGCAGT ATGACCAGCT CACGCCCAAG 
AGATCCAGGA AGTTGAAGGA AGTTCGTCTG GATCGCACAC ACCCCGAGGG TCTGGGTTTG 
AGTGTTCGAG GAGGACTGGA GTTTGGCTCT GGCCTCTTTA TATCGCAGAT AGTCAAAGAT 
GGACAGGCTG GAAATGTCGG CCTGCAGGTC GGTGATGAGA TTGTGCGCAT CAACGGCTAC 
TCCATCTCTT CCTGCATTCA CGAGGAGGTC ATCAACCTCA TCAAGACCAA GAAGACTGTG 
TCTTTGAAAG TCCGCCATGT GGGAATGATT CCTGTCAAGA GCTCCTCCGA TGAGCCTCTG 
AAGTGGCAGT TTGTGGATCA GTTTGTTTCG GAGTCAGGGG AGAAGAGTAA CAGTATTGCT 
GGCTTGGCTT CAATTGGAGG GAATGAAATT AAGGAAAAGA AAGTGTTCCT TAGTTTGGTG 
GGCACCAAAG GCATGGGAAT CAGCATATCA AGTGGACCAA CAAAGAAACC AGGCATCTAC 
GTGAGCAATG TGAAGCCGGG TTCACTCTCA GCTGAAGTGG GCTTACAGGT TGGCGACCAG 
ATTGTGGAGG TCAATGGGGT GGAATTCACC AACCTGAGTC ATCAAGAGGC AGTGCGGGTG 
TTAAAGAGCA GCAGGAGTCT AACCATCACC GTACTCACTG GGGCTGGAAG TGAGCTGTTT 
ATGACGGATG AGGAGCGGTT GGCGGTTGAA GCTCGACATG AGCTGGACAG ACAGGAGTTA 
ATGCATCAGA AGAAAGTGGC ATTAGAGACC AACAAAATTG TCAAAGAACA ACAGGAGAAA 
GAACGCCAGA GGAAGATGGA GATATCTCAG AAGGCTGCAG AGGAAGAAGA GCGCTATCGC 
AAAGAGATGG AAAGAATTGA AGCTGCAGAG AAGAAACACC ACAAAGAGTG GGAAGATGAT 
TGGGGCTCCA AGGAAAAAAA AAAATCTGTT TCCCCGGCCG CTTCTCCATC CCCTCCACCA 
CCCACACGTC TTGCACCTTC ACCCAAACCT AAAACCTCCA CTGCTGATTC CTACGAAGAA 
GAGACCGGAG AAGGGTTTCA GAAATACGTG GATGATTTTG ATCCACATTC CATGTTCACA 
GCCGAACAGA TAGCAGGCAG AGATGTGCGA CTTTTAAGAA TTAAGAAGGA AGGAAAGCTT 
GATCTGGCCA TTGAGGGAGG AATCGATTCC CCATTGGGAA AGATTGTGGT GTCGTCTATC 
TATGAAGGAG GTGCAGCAGA TAAACATGGT GGGGTTGTGT CAGGTGATGA GATCATGGCA 
GTGAATGGAA AGATTTTGAC AGATGTGACT CTAGCTGAAG GACAGACCTC AATGGCTCAG 
GCCTGGAACA GTGGAGGGGA CTGGATTGAC CTGGTCATCG CAGTGTCTCC ACCAAAGGAA 
TATGAAGATG AGGTCCCTAA AGCAGCATCA GTCAGACCCA CTGCAAAGAA AACGGTTTTT 
CAAGGCAGTG CCCCTGTGTA CAGACAGGGA TACGTCCTTC AGATGAAGAC CCCACCATCC 
CCCAACTTCA TCAGCCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296312.1
CDS	111..1871
Translation	MERKVAREFRHKVELLIDNEAEKDYLYDVLRMYHQSMDLPVLVGDLKLVINEPKRLPLFDAIRPLIPLKHQVQYDQLTPKRSRKLKEVRLDRTHPEGLGLSVRGGLEFGSGLFISQIVKDGQAGNVGLQVGDEIVRINGYSISSCIHEEVINLIKTKKTVSLKVRHVGMIPVKSSSDEPLKWQFVDQFVSESGEKSNSIAGLASIGGNEIKEKKVFLSLVGTKGMGISISSGPTKKPGIYVSNVKPGSLSAEVGLQVGDQIVEVNGVEFTNLSHQEAVRVLKSSRSLTITVLTGAGSELFMTDEERLAVEARHELDRQELMHQKKVALETNKIVKEQQEKERQRKMEISQKAAEEEERYRKEMERIEAAEKKHHKEWEDDWGSKEKKKSVSPAASPSPPPPTRLAPSPKPKTSTADSYEEETGEGFQKYVDDFDPHSMFTAEQIAGRDVRLLRIKKEGKLDLAIEGGIDSPLGKIVVSSIYEGGAADKHGGVVSGDEIMAVNGKILTDVTLAEGQTSMAQAWNSGGDWIDLVIAVSPPKEYEDEVPKAASVRPTAKKTVFQGSAPVYRQGYVLQMKTPPSPNFISH

Target ORF information:

RefSeq Version	XM_009298037.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Usher syndrome 1C (ush1c), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298037.3

ORF Insert Sequence:

ATGGAAAGAA AAGTGGCCAG GGAATTTCGG CATAAGGTGG AGTTGCTTAT TGATAATGAA 
GCAGAGAAGG ACTACCTTTA TGATGTTCTT CGCATGTACC ATCAATCCAT GGACCTGCCT 
GTCCTTGTTG GTGACCTGAA GCTGGTAATA AATGAGCCCA AACGCCTTCC TTTGTTTGAT 
GCCATCAGAC CCTTGATTCC TCTGAAGCAC CAAGTGCAGT ATGACCAGCT CACGCCCAAG 
AGATCCAGGA AGTTGAAGGA AGTTCGTCTG GATCGCACAC ACCCCGAGGG TCTGGGTTTG 
AGTGTTCGAG GAGGACTGGA GTTTGGCTCT GGCCTCTTTA TATCGCAGAT AGTCAAAGAT 
GGACAGGCTG GAAATGTCGG CCTGCAGGTC GGTGATGAGA TTGTGCGCAT CAACGGCTAC 
TCCATCTCTT CCTGCATTCA CGAGGAGGTC ATCAACCTCA TCAAGACCAA GAAGACTGTG 
TCTTTGAAAG TCCGCCATGT GGGAATGATT CCTGTCAAGA GCTCCTCCGA TGAGCCTCTG 
AAGTGGCAGT TTGTGGATCA GTTTGTTTCG GAGTCAGGGG AGAAGAGTAA CAGTATTGCT 
GGCTTGGCTT CAATTGGAGG GAATGAAATT AAGGAAAAGA AAGTGTTCCT TAGTTTGGTG 
GGCACCAAAG GCATGGGAAT CAGCATATCA AGTGGACCAA CAAAGAAACC AGGCATCTAC 
GTGAGCAATG TGAAGCCGGG TTCACTCTCA GCTGAAGTGG GCTTACAGGT TGGCGACCAG 
ATTGTGGAGG TCAATGGGGT GGAATTCACC AACCTGAGTC ATCAAGAGGC AGTGCGGGTG 
TTAAAGAGCA GCAGGAGTCT AACCATCACC GTACTCACTG GGGCTGGAAG TGAGCTGTTT 
ATGACGGATG AGGAGCGGTT GGCGGTTGAA GCTCGACATG AGCTGGACAG ACAGGAGTTA 
ATGCATCAGA AGAAAGTGGC ATTAGAGACC AACAAAATTG TCAAAGAACA ACAGGAGAAA 
GAACGCCAGA GGAAGATGGA GATATCTCAG AAGGCTGCAG AGGAAGAAGA GCGCTATCGC 
AAAGAGATGG AAAGAATTGA AGCTGCAGAG AAGAAACACC ACAAAGAGTG GGAAGATGAT 
TGGGGCTCCA AGGAAAAAAA AAAATCTGTT TCCCCGGCCG CTTCTCCATC CCCTCCACCA 
CCCACACGTC TTGCACCTTC ACCCAAACCT AAAACCTCCA CTGCTGATTC CTACGAAGAA 
GAGACCGGAG AAGGGTTTCA GAAATACGTG GATGATTTTG ATCCACATTC CATGTTCACA 
GCCGAACAGA TAGCAGGCAG AGATGTGCGA CTTTTAAGAA TTAAGAAGGA AGGAAAGCTT 
GATCTGGCCA TTGAGGGAGG AATCGATTCC CCATTGGGAA AGATTGTGGT GTCGTCTATC 
TATGAAGGAG GTGCAGCAGA TAAACATGGT GGGGTTGTGT CAGGTGATGA GATCATGGCA 
GTGAATGGAA AGATTTTGAC AGATGTGACT CTAGCTGAAG GACAGACCTC AATGGCTCAG 
GCCTGGAACA GTGGAGGGGA CTGGATTGAC CTGGTCATCG CAGTGTCTCC ACCAAAGGAA 
TATGAAGATG AGGTCCCTAA AGCAGCATCA GTCAGACCCA CTGCAAAGAA AACGGTTTTT 
CAAGGCAGTG CCCCTGTGTA CAGACAGGGA TACGTCCTTC AGATGAAGAC CCCACCATCC 
CCCAACTTCA TCAGCCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ush1c ( XM_009298038.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296313.1 Danio rerio Usher syndrome 1C (ush1c), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa44328
Clone ID Related Accession (Same CDS sequence)	XM_009298037.2 , XM_009298038.2 , XM_009298037.3 , XM_009298038.3
Accession Version	XM_009298038.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1761bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	harmonin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298038.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGAA AAGTGGCCAG GGAATTTCGG CATAAGGTGG AGTTGCTTAT TGATAATGAA 
GCAGAGAAGG ACTACCTTTA TGATGTTCTT CGCATGTACC ATCAATCCAT GGACCTGCCT 
GTCCTTGTTG GTGACCTGAA GCTGGTAATA AATGAGCCCA AACGCCTTCC TTTGTTTGAT 
GCCATCAGAC CCTTGATTCC TCTGAAGCAC CAAGTGCAGT ATGACCAGCT CACGCCCAAG 
AGATCCAGGA AGTTGAAGGA AGTTCGTCTG GATCGCACAC ACCCCGAGGG TCTGGGTTTG 
AGTGTTCGAG GAGGACTGGA GTTTGGCTCT GGCCTCTTTA TATCGCAGAT AGTCAAAGAT 
GGACAGGCTG GAAATGTCGG CCTGCAGGTC GGTGATGAGA TTGTGCGCAT CAACGGCTAC 
TCCATCTCTT CCTGCATTCA CGAGGAGGTC ATCAACCTCA TCAAGACCAA GAAGACTGTG 
TCTTTGAAAG TCCGCCATGT GGGAATGATT CCTGTCAAGA GCTCCTCCGA TGAGCCTCTG 
AAGTGGCAGT TTGTGGATCA GTTTGTTTCG GAGTCAGGGG AGAAGAGTAA CAGTATTGCT 
GGCTTGGCTT CAATTGGAGG GAATGAAATT AAGGAAAAGA AAGTGTTCCT TAGTTTGGTG 
GGCACCAAAG GCATGGGAAT CAGCATATCA AGTGGACCAA CAAAGAAACC AGGCATCTAC 
GTGAGCAATG TGAAGCCGGG TTCACTCTCA GCTGAAGTGG GCTTACAGGT TGGCGACCAG 
ATTGTGGAGG TCAATGGGGT GGAATTCACC AACCTGAGTC ATCAAGAGGC AGTGCGGGTG 
TTAAAGAGCA GCAGGAGTCT AACCATCACC GTACTCACTG GGGCTGGAAG TGAGCTGTTT 
ATGACGGATG AGGAGCGGTT GGCGGTTGAA GCTCGACATG AGCTGGACAG ACAGGAGTTA 
ATGCATCAGA AGAAAGTGGC ATTAGAGACC AACAAAATTG TCAAAGAACA ACAGGAGAAA 
GAACGCCAGA GGAAGATGGA GATATCTCAG AAGGCTGCAG AGGAAGAAGA GCGCTATCGC 
AAAGAGATGG AAAGAATTGA AGCTGCAGAG AAGAAACACC ACAAAGAGTG GGAAGATGAT 
TGGGGCTCCA AGGAAAAAAA AAAATCTGTT TCCCCGGCCG CTTCTCCATC CCCTCCACCA 
CCCACACGTC TTGCACCTTC ACCCAAACCT AAAACCTCCA CTGCTGATTC CTACGAAGAA 
GAGACCGGAG AAGGGTTTCA GAAATACGTG GATGATTTTG ATCCACATTC CATGTTCACA 
GCCGAACAGA TAGCAGGCAG AGATGTGCGA CTTTTAAGAA TTAAGAAGGA AGGAAAGCTT 
GATCTGGCCA TTGAGGGAGG AATCGATTCC CCATTGGGAA AGATTGTGGT GTCGTCTATC 
TATGAAGGAG GTGCAGCAGA TAAACATGGT GGGGTTGTGT CAGGTGATGA GATCATGGCA 
GTGAATGGAA AGATTTTGAC AGATGTGACT CTAGCTGAAG GACAGACCTC AATGGCTCAG 
GCCTGGAACA GTGGAGGGGA CTGGATTGAC CTGGTCATCG CAGTGTCTCC ACCAAAGGAA 
TATGAAGATG AGGTCCCTAA AGCAGCATCA GTCAGACCCA CTGCAAAGAA AACGGTTTTT 
CAAGGCAGTG CCCCTGTGTA CAGACAGGGA TACGTCCTTC AGATGAAGAC CCCACCATCC 
CCCAACTTCA TCAGCCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296313.1
CDS	111..1871
Translation	MERKVAREFRHKVELLIDNEAEKDYLYDVLRMYHQSMDLPVLVGDLKLVINEPKRLPLFDAIRPLIPLKHQVQYDQLTPKRSRKLKEVRLDRTHPEGLGLSVRGGLEFGSGLFISQIVKDGQAGNVGLQVGDEIVRINGYSISSCIHEEVINLIKTKKTVSLKVRHVGMIPVKSSSDEPLKWQFVDQFVSESGEKSNSIAGLASIGGNEIKEKKVFLSLVGTKGMGISISSGPTKKPGIYVSNVKPGSLSAEVGLQVGDQIVEVNGVEFTNLSHQEAVRVLKSSRSLTITVLTGAGSELFMTDEERLAVEARHELDRQELMHQKKVALETNKIVKEQQEKERQRKMEISQKAAEEEERYRKEMERIEAAEKKHHKEWEDDWGSKEKKKSVSPAASPSPPPPTRLAPSPKPKTSTADSYEEETGEGFQKYVDDFDPHSMFTAEQIAGRDVRLLRIKKEGKLDLAIEGGIDSPLGKIVVSSIYEGGAADKHGGVVSGDEIMAVNGKILTDVTLAEGQTSMAQAWNSGGDWIDLVIAVSPPKEYEDEVPKAASVRPTAKKTVFQGSAPVYRQGYVLQMKTPPSPNFISH

Target ORF information:

RefSeq Version	XM_009298038.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio Usher syndrome 1C (ush1c), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298038.3

ORF Insert Sequence:

ATGGAAAGAA AAGTGGCCAG GGAATTTCGG CATAAGGTGG AGTTGCTTAT TGATAATGAA 
GCAGAGAAGG ACTACCTTTA TGATGTTCTT CGCATGTACC ATCAATCCAT GGACCTGCCT 
GTCCTTGTTG GTGACCTGAA GCTGGTAATA AATGAGCCCA AACGCCTTCC TTTGTTTGAT 
GCCATCAGAC CCTTGATTCC TCTGAAGCAC CAAGTGCAGT ATGACCAGCT CACGCCCAAG 
AGATCCAGGA AGTTGAAGGA AGTTCGTCTG GATCGCACAC ACCCCGAGGG TCTGGGTTTG 
AGTGTTCGAG GAGGACTGGA GTTTGGCTCT GGCCTCTTTA TATCGCAGAT AGTCAAAGAT 
GGACAGGCTG GAAATGTCGG CCTGCAGGTC GGTGATGAGA TTGTGCGCAT CAACGGCTAC 
TCCATCTCTT CCTGCATTCA CGAGGAGGTC ATCAACCTCA TCAAGACCAA GAAGACTGTG 
TCTTTGAAAG TCCGCCATGT GGGAATGATT CCTGTCAAGA GCTCCTCCGA TGAGCCTCTG 
AAGTGGCAGT TTGTGGATCA GTTTGTTTCG GAGTCAGGGG AGAAGAGTAA CAGTATTGCT 
GGCTTGGCTT CAATTGGAGG GAATGAAATT AAGGAAAAGA AAGTGTTCCT TAGTTTGGTG 
GGCACCAAAG GCATGGGAAT CAGCATATCA AGTGGACCAA CAAAGAAACC AGGCATCTAC 
GTGAGCAATG TGAAGCCGGG TTCACTCTCA GCTGAAGTGG GCTTACAGGT TGGCGACCAG 
ATTGTGGAGG TCAATGGGGT GGAATTCACC AACCTGAGTC ATCAAGAGGC AGTGCGGGTG 
TTAAAGAGCA GCAGGAGTCT AACCATCACC GTACTCACTG GGGCTGGAAG TGAGCTGTTT 
ATGACGGATG AGGAGCGGTT GGCGGTTGAA GCTCGACATG AGCTGGACAG ACAGGAGTTA 
ATGCATCAGA AGAAAGTGGC ATTAGAGACC AACAAAATTG TCAAAGAACA ACAGGAGAAA 
GAACGCCAGA GGAAGATGGA GATATCTCAG AAGGCTGCAG AGGAAGAAGA GCGCTATCGC 
AAAGAGATGG AAAGAATTGA AGCTGCAGAG AAGAAACACC ACAAAGAGTG GGAAGATGAT 
TGGGGCTCCA AGGAAAAAAA AAAATCTGTT TCCCCGGCCG CTTCTCCATC CCCTCCACCA 
CCCACACGTC TTGCACCTTC ACCCAAACCT AAAACCTCCA CTGCTGATTC CTACGAAGAA 
GAGACCGGAG AAGGGTTTCA GAAATACGTG GATGATTTTG ATCCACATTC CATGTTCACA 
GCCGAACAGA TAGCAGGCAG AGATGTGCGA CTTTTAAGAA TTAAGAAGGA AGGAAAGCTT 
GATCTGGCCA TTGAGGGAGG AATCGATTCC CCATTGGGAA AGATTGTGGT GTCGTCTATC 
TATGAAGGAG GTGCAGCAGA TAAACATGGT GGGGTTGTGT CAGGTGATGA GATCATGGCA 
GTGAATGGAA AGATTTTGAC AGATGTGACT CTAGCTGAAG GACAGACCTC AATGGCTCAG 
GCCTGGAACA GTGGAGGGGA CTGGATTGAC CTGGTCATCG CAGTGTCTCC ACCAAAGGAA 
TATGAAGATG AGGTCCCTAA AGCAGCATCA GTCAGACCCA CTGCAAAGAA AACGGTTTTT 
CAAGGCAGTG CCCCTGTGTA CAGACAGGGA TACGTCCTTC AGATGAAGAC CCCACCATCC 
CCCAACTTCA TCAGCCACTA G

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ush1c ( XM_017354085.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209574.1 Danio rerio Usher syndrome 1C (ush1c), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa57802
Clone ID Related Accession (Same CDS sequence)	XM_017354085.1
Accession Version	XM_017354085.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	harmonin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGAA AAGTGGCCAG GGAATTTCGG CATAAGGTGG AGTTGCTTAT TGATAATGAA 
GCAGAGAAGG ACTACCTTTA TGATGTTCTT CGCATGTACC ATCAATCCAT GGACCTGCCT 
GTCCTTGTTG GTGACCTGAA GCTGGTAATA AATGAGCCCA AACGCCTTCC TTTGTTTGAT 
GCCATCAGAC CCTTGATTCC TCTGAAGCAC CAAGTGCAGT ATGACCAGCT CACGCCCAAG 
AGATCCAGGA AGTTGAAGGA AGTTCGTCTG GATCGCACAC ACCCCGAGGG TCTGGGTTTG 
AGTGTTCGAG GAGGACTGGA GTTTGGCTCT GGCCTCTTTA TATCGCAGAT AGTCAAAGAT 
GGACAGGCTG GAAATGTCGG CCTGCAGGTC GGTGATGAGA TTGTGCGCAT CAACGGCTAC 
TCCATCTCTT CCTGCATTCA CGAGGAGGTC ATCAACCTCA TCAAGACCAA GAAGACTGTG 
TCTTTGAAAG TCCGCCATGT GGGAATGATT CCTGTCAAGA GCTCCTCCGA TGAGCCTCTG 
AAGTGGCAGT TTGTGGATCA GTTTGTTTCG GAGTCAGGGG AGAAGAGTAA CAGTATTGCT 
GGCTTGGCTT CAATTGGAGG GAATGAAATT AAGGAAAAGA AAGTGTTCCT TAGTTTGGTG 
GGCACCAAAG GCATGGGAAT CAGCATATCA AGTGGACCAA CAAAGAAACC AGGCATCTAC 
GTGAGCAATG TGAAGCCGGG TTCACTCTCA GCTGAAGTGG GCTTACAGGT TGGCGACCAG 
ATTGTGGAGG TCAATGGGGT GGAATTCACC AACCTGAGTC ATCAAGAGGC AGTGCGGGTG 
TTAAAGAGCA GCAGGAGTCT AACCATCACC GTACTCACTG GGGCTGGAAG TGAGCTGTTT 
ATGACGGATG AGGAGCGGTT GGCGGTTGAA GCTCGACATG AGCTGGACAG ACAGGAGTTA 
ATGCATCAGA AGAAAGTGGC ATTAGAGACC AACAAAATTG TCAAAGAACA ACAGGAGAAA 
GAACGCCAGA GGAAGATGGA GATATCTCAG AAGGCTGCAG AGGAAGAAGA GCGCTATCGC 
AAAGAGATGG AAAGAATTGA AGCTGCAGAG AAGAAACACC ACAAAGAGTG GGAAGATGAT 
TGGGGCTCCA AGGAAAAAAA AAAATCTGTT TCCCCGGCCG CTTCTCCATC CCCTCCACCA 
CCCACACGTC TTGCACCTTC ACCCAAACCT AAAACCTCCA CTGCTGATTC CTACGAAGAA 
GAGACCGGAG AACATTCGGT TGGTTCTACC GTTATGAAGG AAAGTTGCCT ACACTCAAAA 
AGAAGGAAAA AGAAACCAAA AAGAAGAAGA AGATGTCCAA GACTGACACT CTTCCAACTG 
AGAAAAAAAG CAAAAAAGAG ATGGAGTTTG AGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209574.1
CDS	110..1525
Translation	MERKVAREFRHKVELLIDNEAEKDYLYDVLRMYHQSMDLPVLVGDLKLVINEPKRLPLFDAIRPLIPLKHQVQYDQLTPKRSRKLKEVRLDRTHPEGLGLSVRGGLEFGSGLFISQIVKDGQAGNVGLQVGDEIVRINGYSISSCIHEEVINLIKTKKTVSLKVRHVGMIPVKSSSDEPLKWQFVDQFVSESGEKSNSIAGLASIGGNEIKEKKVFLSLVGTKGMGISISSGPTKKPGIYVSNVKPGSLSAEVGLQVGDQIVEVNGVEFTNLSHQEAVRVLKSSRSLTITVLTGAGSELFMTDEERLAVEARHELDRQELMHQKKVALETNKIVKEQQEKERQRKMEISQKAAEEEERYRKEMERIEAAEKKHHKEWEDDWGSKEKKKSVSPAASPSPPPPTRLAPSPKPKTSTADSYEEETGEHSVGSTVMKESCLHSKRRKKKPKRRRRCPRLTLFQLRKKAKKRWSLS

Target ORF information:

RefSeq Version	XM_017354085.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Usher syndrome 1C (ush1c), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354085.1

ORF Insert Sequence:

ATGGAAAGAA AAGTGGCCAG GGAATTTCGG CATAAGGTGG AGTTGCTTAT TGATAATGAA 
GCAGAGAAGG ACTACCTTTA TGATGTTCTT CGCATGTACC ATCAATCCAT GGACCTGCCT 
GTCCTTGTTG GTGACCTGAA GCTGGTAATA AATGAGCCCA AACGCCTTCC TTTGTTTGAT 
GCCATCAGAC CCTTGATTCC TCTGAAGCAC CAAGTGCAGT ATGACCAGCT CACGCCCAAG 
AGATCCAGGA AGTTGAAGGA AGTTCGTCTG GATCGCACAC ACCCCGAGGG TCTGGGTTTG 
AGTGTTCGAG GAGGACTGGA GTTTGGCTCT GGCCTCTTTA TATCGCAGAT AGTCAAAGAT 
GGACAGGCTG GAAATGTCGG CCTGCAGGTC GGTGATGAGA TTGTGCGCAT CAACGGCTAC 
TCCATCTCTT CCTGCATTCA CGAGGAGGTC ATCAACCTCA TCAAGACCAA GAAGACTGTG 
TCTTTGAAAG TCCGCCATGT GGGAATGATT CCTGTCAAGA GCTCCTCCGA TGAGCCTCTG 
AAGTGGCAGT TTGTGGATCA GTTTGTTTCG GAGTCAGGGG AGAAGAGTAA CAGTATTGCT 
GGCTTGGCTT CAATTGGAGG GAATGAAATT AAGGAAAAGA AAGTGTTCCT TAGTTTGGTG 
GGCACCAAAG GCATGGGAAT CAGCATATCA AGTGGACCAA CAAAGAAACC AGGCATCTAC 
GTGAGCAATG TGAAGCCGGG TTCACTCTCA GCTGAAGTGG GCTTACAGGT TGGCGACCAG 
ATTGTGGAGG TCAATGGGGT GGAATTCACC AACCTGAGTC ATCAAGAGGC AGTGCGGGTG 
TTAAAGAGCA GCAGGAGTCT AACCATCACC GTACTCACTG GGGCTGGAAG TGAGCTGTTT 
ATGACGGATG AGGAGCGGTT GGCGGTTGAA GCTCGACATG AGCTGGACAG ACAGGAGTTA 
ATGCATCAGA AGAAAGTGGC ATTAGAGACC AACAAAATTG TCAAAGAACA ACAGGAGAAA 
GAACGCCAGA GGAAGATGGA GATATCTCAG AAGGCTGCAG AGGAAGAAGA GCGCTATCGC 
AAAGAGATGG AAAGAATTGA AGCTGCAGAG AAGAAACACC ACAAAGAGTG GGAAGATGAT 
TGGGGCTCCA AGGAAAAAAA AAAATCTGTT TCCCCGGCCG CTTCTCCATC CCCTCCACCA 
CCCACACGTC TTGCACCTTC ACCCAAACCT AAAACCTCCA CTGCTGATTC CTACGAAGAA 
GAGACCGGAG AACATTCGGT TGGTTCTACC GTTATGAAGG AAAGTTGCCT ACACTCAAAA 
AGAAGGAAAA AGAAACCAAA AAGAAGAAGA AGATGTCCAA GACTGACACT CTTCCAACTG 
AGAAAAAAAG CAAAAAAGAG ATGGAGTTTG AGTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ush1c ( XM_017354086.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209575.1 Danio rerio Usher syndrome 1C (ush1c), transcript variant X4, mRNA.

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CloneID	ODa57803
Clone ID Related Accession (Same CDS sequence)	XM_017354086.1
Accession Version	XM_017354086.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1251bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	harmonin isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAGAA AAGTGGCCAG GGAATTTCGG CATAAGGTGG AGTTGCTTAT TGATAATGAA 
GCAGAGAAGG ACTACCTTTA TGATGTTCTT CGCATGTACC ATCAATCCAT GGACCTGCCT 
GTCCTTGTTG GTGACCTGAA GCTGGTAATA AATGAGCCCA AACGCCTTCC TTTGTTTGAT 
GCCATCAGAC CCTTGATTCC TCTGAAGCAC CAAGTGCAGT ATGACCAGCT CACGCCCAAG 
AGATCCAGGA AGTTGAAGGA AGTTCGTCTG GATCGCACAC ACCCCGAGGG TCTGGGTTTG 
AGTGTTCGAG GAGGACTGGA GTTTGGCTCT GGCCTCTTTA TATCGCAGAT AGTCAAAGAT 
GGACAGGCTG GAAATGTCGG CCTGCAGGTC GGTGATGAGA TTGTGCGCAT CAACGGCTAC 
TCCATCTCTT CCTGCATTCA CGAGGAGGTC ATCAACCTCA TCAAGACCAA GAAGACTGTG 
TCTTTGAAAG TCCGCCATGT GGGAATGATT CCTGTCAAGA GCTCCTCCGA TGAGCCTCTG 
AAGTGGCAGT TTGTGGATCA GTTTGTTTCG GAGTCAGGGG AGAAGAGTAA CAGTATTGCT 
GGCTTGGCTT CAATTGGAGG GAATGAAATT AAGGAAAAGA AAGTGTTCCT TAGTTTGGTG 
GGCACCAAAG GCATGGGAAT CAGCATATCA AGTGGACCAA CAAAGAAACC AGGCATCTAC 
GTGAGCAATG TGAAGCCGGG TTCACTCTCA GCTGAAGTGG GCTTACAGGT TGGCGACCAG 
ATTGTGGAGG TCAATGGGGT GGAATTCACC AACCTGAGTC ATCAAGAGGC AGTGCGGGTG 
TTAAAGAGCA GCAGGAGTCT AACCATCACC GTACTCACTG GGGCTGGAAG TGAGCTGTTT 
ATGACGGATG AGGAGCGGTT GGCGGTTGAA GCTCGACATG AGCTGGACAG ACAGGAGTTA 
ATGCATCAGA AGAAAGTGGC ATTAGAGACC AACAAAATTG TCAAAGAACA ACAGGAGAAA 
GAACGCCAGA GGAAGATGGA GATATCTCAG AAGGCTGCAG AGGAAGAAGA GCGCTATCGC 
AAAGAGATGG AAAGAATTGA AGCTGCAGAG AAGAAACACC ACAAAGAGTG GGAAGATGAT 
TGGGGCTCCA AGGAAAAAAA AAAATCTGTT TCCCCGGCCG CTTCTCCATC CCCTCCACCA 
CCCACACGTC TTGCACCTTC ACCCAAACCT AAAACCTCCA ATGAAATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209575.1
CDS	110..1360
Translation	MERKVAREFRHKVELLIDNEAEKDYLYDVLRMYHQSMDLPVLVGDLKLVINEPKRLPLFDAIRPLIPLKHQVQYDQLTPKRSRKLKEVRLDRTHPEGLGLSVRGGLEFGSGLFISQIVKDGQAGNVGLQVGDEIVRINGYSISSCIHEEVINLIKTKKTVSLKVRHVGMIPVKSSSDEPLKWQFVDQFVSESGEKSNSIAGLASIGGNEIKEKKVFLSLVGTKGMGISISSGPTKKPGIYVSNVKPGSLSAEVGLQVGDQIVEVNGVEFTNLSHQEAVRVLKSSRSLTITVLTGAGSELFMTDEERLAVEARHELDRQELMHQKKVALETNKIVKEQQEKERQRKMEISQKAAEEEERYRKEMERIEAAEKKHHKEWEDDWGSKEKKKSVSPAASPSPPPPTRLAPSPKPKTSNEI

Target ORF information:

RefSeq Version	XM_017354086.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio Usher syndrome 1C (ush1c), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017354086.1

ORF Insert Sequence:

ATGGAAAGAA AAGTGGCCAG GGAATTTCGG CATAAGGTGG AGTTGCTTAT TGATAATGAA 
GCAGAGAAGG ACTACCTTTA TGATGTTCTT CGCATGTACC ATCAATCCAT GGACCTGCCT 
GTCCTTGTTG GTGACCTGAA GCTGGTAATA AATGAGCCCA AACGCCTTCC TTTGTTTGAT 
GCCATCAGAC CCTTGATTCC TCTGAAGCAC CAAGTGCAGT ATGACCAGCT CACGCCCAAG 
AGATCCAGGA AGTTGAAGGA AGTTCGTCTG GATCGCACAC ACCCCGAGGG TCTGGGTTTG 
AGTGTTCGAG GAGGACTGGA GTTTGGCTCT GGCCTCTTTA TATCGCAGAT AGTCAAAGAT 
GGACAGGCTG GAAATGTCGG CCTGCAGGTC GGTGATGAGA TTGTGCGCAT CAACGGCTAC 
TCCATCTCTT CCTGCATTCA CGAGGAGGTC ATCAACCTCA TCAAGACCAA GAAGACTGTG 
TCTTTGAAAG TCCGCCATGT GGGAATGATT CCTGTCAAGA GCTCCTCCGA TGAGCCTCTG 
AAGTGGCAGT TTGTGGATCA GTTTGTTTCG GAGTCAGGGG AGAAGAGTAA CAGTATTGCT 
GGCTTGGCTT CAATTGGAGG GAATGAAATT AAGGAAAAGA AAGTGTTCCT TAGTTTGGTG 
GGCACCAAAG GCATGGGAAT CAGCATATCA AGTGGACCAA CAAAGAAACC AGGCATCTAC 
GTGAGCAATG TGAAGCCGGG TTCACTCTCA GCTGAAGTGG GCTTACAGGT TGGCGACCAG 
ATTGTGGAGG TCAATGGGGT GGAATTCACC AACCTGAGTC ATCAAGAGGC AGTGCGGGTG 
TTAAAGAGCA GCAGGAGTCT AACCATCACC GTACTCACTG GGGCTGGAAG TGAGCTGTTT 
ATGACGGATG AGGAGCGGTT GGCGGTTGAA GCTCGACATG AGCTGGACAG ACAGGAGTTA 
ATGCATCAGA AGAAAGTGGC ATTAGAGACC AACAAAATTG TCAAAGAACA ACAGGAGAAA 
GAACGCCAGA GGAAGATGGA GATATCTCAG AAGGCTGCAG AGGAAGAAGA GCGCTATCGC 
AAAGAGATGG AAAGAATTGA AGCTGCAGAG AAGAAACACC ACAAAGAGTG GGAAGATGAT 
TGGGGCTCCA AGGAAAAAAA AAAATCTGTT TCCCCGGCCG CTTCTCCATC CCCTCCACCA 
CCCACACGTC TTGCACCTTC ACCCAAACCT AAAACCTCCA ATGAAATTTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

High-throughput gene targeting and phenotyping in zebrafish using CRISPR/Cas9.
Genome research25(7)1030-42(2015 Jul)
Varshney GK,Pei W,LaFave MC,Idol J,Xu L,Gallardo V,Carrington B,Bishop K,Jones M,Li M,Harper U,Huang SC,Prakash A,Chen W,Sood R,Ledin J,Burgess SM

Complexes of Usher proteins preassemble at the endoplasmic reticulum and are required for trafficking and ER homeostasis.
Disease models & mechanisms7(5)547-59(2014 May)
Blanco-S?nchez B,Cl?ment A,Fierro J Jr,Washbourne P,Westerfield M

Harmonin (Ush1c) is required in zebrafish M?ller glial cells for photoreceptor synaptic development and function.
Disease models & mechanisms4(6)786-800(2011 Nov)
Phillips JB,Blanco-Sanchez B,Lentz JJ,Tallafuss A,Khanobdee K,Sampath S,Jacobs ZG,Han PF,Mishra M,Titus TA,Williams DS,Keats BJ,Washbourne P,Westerfield M

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ush1c cDNA ORF clone, Danio rerio(zebrafish)

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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