Ush1c cDNA ORF clone, Mus musculus(house mouse)

Gene
Clones
gRNAs

Summary

Gene Symbol	Ush1c
Entrez Gene ID	72088
Full Name	USH1 protein network component harmonin
Synonyms	2010016F01Rik,harmonin
General protein information	Preferred Names USH1 protein network component harmonin Names harmonin PDZ domain-containing protein Usher syndrome 1C homolog usher syndrome type-1C protein homolog
Gene Type	protein-coding
Organism	Mus musculus(house mouse)
Genome	Chromosome: 7 Map Location: 7 B3\|7 29.66 cM

mRNA and Protein(s)

mRNA	Protein	Name
NM_153677.2	NP_710143.2	harmonin isoform b3
NM_001291182.1	NP_001278111.1	harmonin isoform b2
XM_006541212.3	XP_006541275.1	harmonin isoform X1
NM_023649.2	NP_076138.2	harmonin isoform a1
NM_001163733.1	NP_001157205.1	harmonin isoform b4

Pathways from BioSystems

Homologies

Homo sapiens (human)	USH1C	NP_710142.1
Bos taurus (cattle)	USH1C	NP_001030459.1
Danio rerio (zebrafish)	ush1c	NP_001035018.1
Xenopus tropicalis (tropical clawed frog)	ush1c	NP_001096522.1
Pan troglodytes (chimpanzee)	USH1C	XP_003951893.1
Canis lupus familiaris (dog)	USH1C	XP_005633777.1
Mus musculus (house mouse)	Ush1c	NP_710143.2
Gallus gallus (chicken)	USH1C	XP_421006.4

Gene Ontology
Bibliography

Related articles in PubMed

Antisense oligonucleotide therapy rescues disruptions in organization of exploratory movements associated with Usher syndrome type 1C in mice.
Donaldson TN, Jennings KT, Cherep LA, McNeela AM, Depreux FF, Jodelka FM, Hastings ML, Wallace DG
Behavioural brain research33876-87(2018 Feb)

Rescue of peripheral vestibular function in Usher syndrome mice using a splice-switching antisense oligonucleotide.
Vijayakumar S, Depreux FF, Jodelka FM, Lentz JJ, Rigo F, Jones TA, Hastings ML
Human molecular genetics26(18)3482-3494(2017 Sep)

The roles of USH1 proteins and PDZ domain-containing USH proteins in USH2 complex integrity in cochlear hair cells.
Zou J, Chen Q, Almishaal A, Mathur PD, Zheng T, Tian C, Zheng QY, Yang J
Human molecular genetics26(3)624-636(2017 02)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Harmonin enhances voltage-dependent facilitation of Cav1.3 channels and synchronous exocytosis in mouse inner hair cells.
Title: Harmonin enhances voltage-dependent facilitation of Cav1.3 channels and synchronous exocytosis in mouse inner hair cells.

MYO7A, USH1G (Sans) and CDH23 form the upper tip-link complex in adult mice, likely in combination with USH1C (harmonin).
Title: Digenic inheritance of deafness caused by 8J allele of myosin-VIIA and mutations in other Usher I genes.

examine the effects of null mutation of the Ush1c gene on subcellular localization of Myo7a, Pcdh15 and Sans in the inner ear
Title: Analysis of subcellular localization of Myo7a, Pcdh15 and Sans in Ush1c knockout mice.

Cadherin-23, myosin VIIa and harmonin form a ternary complex and interact with phospholipids.
Title: Cadherin-23, myosin VIIa and harmonin, encoded by Usher syndrome type I genes, form a ternary complex and interact with membrane phospholipids.

The fact that Ush1c expression is much higher in the ear than in the eye suggests a different role for Ush1c in ear function than in the eye and may explain why Ush1c mutant mice do not recapitulate vision defects.
Title: Ush1c gene expression levels in the ear and eye suggest different roles for Ush1c in neurosensory organs in a new Ush1c knockout mouse.

The following Ush1c gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the Ush1c cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OMu15497	NM_001291182.1 Latest version!	Mus musculus USH1 protein network component harmonin (Ush1c), transcript variant b2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$475.30 $679.00
OMu39045	XM_006541212.3 Latest version!	Mus musculus USH1 protein network component harmonin (Ush1c), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$307.30 $439.00
OMu15505	NM_153677.2 Latest version!	Mus musculus USH1 protein network component harmonin (Ush1c), transcript variant b3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$531.30 $759.00
OMu15515	NM_001163733.1 Latest version!	Mus musculus USH1 protein network component harmonin (Ush1c), transcript variant b4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$475.30 $679.00
OMu15513	NM_023649.2 Latest version!	Mus musculus USH1 protein network component harmonin (Ush1c), transcript variant a1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	5-7	$321.30 $459.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

Ush1c ( NM_001291182.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001278111.1 Mus musculus USH1 protein network component harmonin (Ush1c), transcript variant b2, mRNA.

Price & Availability↑

CloneID	OMu15497
Clone ID Related Accession (Same CDS sequence)	NM_001291182.1
Accession Version	NM_001291182.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2580bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-23
Organism	Mus musculus(house mouse)
Product	harmonin isoform b2
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AC120135.7. Transcript Variant: This variant (b2) uses an alternate splice junction at the 5' end of an exon compared to variant b3, that causes a frameshift. The resulting isoform (b2) has a shorter and distinct C-terminus compared to isoform b3. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY103465.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849376 [ECO:0000350] ##Evidence-Data-END## ##RefSeq-Attributes-START## NMD candidate :: translation inferred ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGACCGGA AGGTGGCCCG AGAATTCCGA CACAAGGTGG ATTTTCTCAT TGAAAATGAC 
GCAGAGAAGG ACTATCTTTA TGATGTGCTG CGGATGTATC ACCAGACCAT GGATGTGGCT 
GTGCTCGTGG GAGACCTGAA GCTGGTCATC AATGAACCCA ACCGCCTGCC GCTGTTTGAT 
GCCATTCGAC CCCTGATCCC ACTGAAGCAC CAGGTGGAGT ATGACCAGCT GACACCCCGA 
CGCTCCAGGA AACTGAAGGA GGTACGCTTG GACCGTCTGC ACCCAGAAGG TCTCGGCCTC 
AGCGTGCGTG GAGGCCTGGA ATTTGGCTGT GGACTCTTTA TCTCCCACCT CATCAAAGGT 
GGCCAGGCAG ACAGCGTTGG GCTTCAGGTA GGGGATGAAA TTGTCCGGAT CAACGGCTAT 
TCCATCTCTT CCTGTACCCA TGAGGAAGTC ATCAACCTGA TCCGCACCAA GAAGACCGTG 
TCCATCAAAG TGAGACACAT CGGACTGATC CCTGTGAAGA GCTCTCCTGA GGAGTCCCTC 
AAATGGCAGT ATGTGGATCA GTTCGTGTCG GAATCTGGGG GTGTGCGAGG TGGCTTGGGC 
TCACCTGGCA ATCGGACAAC CAAGGAGAAG AAGGTGTTTA TCAGTCTAGT GGGCTCTCGG 
GGCCTGGGCT GCAGCATCTC CAGTGGCCCC ATCCAGAAGC CTGGCATCTT CGTCAGCCAC 
GTGAAGCCTG GCTCCCTGTC TGCAGAGGTG GGGTTAGAGA CAGGAGACCA GATTGTGGAA 
GTCAACGGCA TAGACTTCAC CAACCTGGAC CACAAGGAGG CTGTGAATGT CCTGAAGAGC 
AGCCGCAGCC TGACCATCTC CATCGTTGCT GGAGCCGGCC GGGAGCTGTT CATGACGGAC 
CGGGAACGGC TGGAGGAGGC ACGGCAGCGT GAGCTGCAAC GGCAGGAACT CCTCATGCAG 
AAGCGGCTGG CCATGGAGTC CAACAAGATC CTCCAGGAGC AGCAGGAGAT GGAGCGCCAG 
AGGAGAAAGG AGATCGCCCA GAAGGCTGCC GAGGAGAATG AGAGATACCG GAAGGAGATG 
GAACAGATCT CGGAGGAGGA AGAGAAGTTT AAGAAGCAGT GGAAAGAAGA CTGGGGTTCA 
AAGGAGCAGC TCATTCTGCC TAAGACCATC ACCGCAGAGG TGCACCCGGT GCCCCTTCGC 
AAGCCCAAGT CTTTTGGGTG GTTTTACCGT TACGATGGCA AATTCCCAAC CATCCGGAAG 
AAAGCAAAAG AGAAGAAGAA AGCCAAGTAC GATAGCCTGC AGGACCTGAG GAAGAACAAG 
AAGGAACTCG AGTTTGAACA AAAGCTCTAC AAAGAGAAGG AAGAGATGCT GGAGAAGGAG 
AAGCAGCTCA AGATCAACCG GCTGGCCCAG GAGGTGTCTG AGACTGAGCG GGAAGACCTT 
GAGGAATCGG AAAAGACTCA GTATTGGGTG GAGAGGCTCT GTCAGACGCG CCTCGAGCAG 
ATTTCCTCTG CAGAGAATGA GATTCCTGAG ATGACCACAG GGCCCCCACC TCCCCCACCT 
TCTGTGTCTC CTCTGGCCCC ACCCTTGAGA CGATTCGCTG GTGGGATCCA CCTCCACACC 
ACTGACCTGG ATGACATCCC CTTGGACATG TTCTACTACC CCCCCAAGAC TCCCTCAGCA 
CTGCCCGTGA TGCCCCACCC TCCATCTGTC AACCCACCTT CCAAGGTCCC CGCGCCCCCT 
GTGCTTCCCT CATCAGGTCA CGTGAGCAGC TCATCCTCAC CATGGGTGCA ACGCACTCCT 
CCCCCAATCC CCATTCCTCC GCCTCCATCC ATTCCCACCC AAGACCTCAC TCCTACCCGC 
CCATTGCCCT CAGCACTGGA AGAGGCCCTG GGCAACCACC CCTTCCGCAC TGGGGACCCA 
GGCCATCCAG CAGATGACTG GGAGGCAAAC ACCCACAGTG GGAAGCCCTC CAGCTCCCCC 
ACCACTGAGC GGAGCTTCCC ACCTGCCCCA AAGACATTCT GCCCAAGCCC ACAGCCTCCA 
CGAGGCCCTG GCGTGTCCAC CATCTCCAAA CCCGTCATGG TCCACCAGGA ACACAACTTC 
GTCTACAGGC CTGCTGTGAA GTCCGAGGTT CTGCCGCAGG AGATGCTGAA GAGGATGGTG 
GTATATCAGA CAGCATTCAG ACAAGATTTT CGAAAATACG AAGAAGGCTT TGATCCCTAC 
TCCATGTTCT CGCCAGAGCA GATCGCAGGG AAGGACGTCC GGCTTCTGCG AATCAAGAAG 
GAGGGGTCTT TAGACTTGGC CCTGGAGGGA GGCGTGGACT CCCCCGTGGG GAAGGTGGTC 
GTCTCTGCTG TTTATGAAGG GGGAGCTGCG GAGAGGCATG GTGGCGTTGT GAAAGGGGAT 
GAGATCATGG CCATCAATGG CAAGATTGTG ACGGACTACA CCCTGGCTGA GGCCGAGGCT 
GCCCTACAGA AGGCCTGGAA TCAGGGCGGG GACTGGATAG ACCTTGTGGT TGCTGTCTGT 
CCCCCTAAGG AGTATGATGA CGAGCTCCGA AAGCCCCCAG CTGGCCCGAA AGCAGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001278111.1
CDS	27..2606
Translation	MDRKVAREFRHKVDFLIENDAEKDYLYDVLRMYHQTMDVAVLVGDLKLVINEPNRLPLFDAIRPLIPLKHQVEYDQLTPRRSRKLKEVRLDRLHPEGLGLSVRGGLEFGCGLFISHLIKGGQADSVGLQVGDEIVRINGYSISSCTHEEVINLIRTKKTVSIKVRHIGLIPVKSSPEESLKWQYVDQFVSESGGVRGGLGSPGNRTTKEKKVFISLVGSRGLGCSISSGPIQKPGIFVSHVKPGSLSAEVGLETGDQIVEVNGIDFTNLDHKEAVNVLKSSRSLTISIVAGAGRELFMTDRERLEEARQRELQRQELLMQKRLAMESNKILQEQQEMERQRRKEIAQKAAEENERYRKEMEQISEEEEKFKKQWKEDWGSKEQLILPKTITAEVHPVPLRKPKSFGWFYRYDGKFPTIRKKAKEKKKAKYDSLQDLRKNKKELEFEQKLYKEKEEMLEKEKQLKINRLAQEVSETEREDLEESEKTQYWVERLCQTRLEQISSAENEIPEMTTGPPPPPPSVSPLAPPLRRFAGGIHLHTTDLDDIPLDMFYYPPKTPSALPVMPHPPSVNPPSKVPAPPVLPSSGHVSSSSSPWVQRTPPPIPIPPPPSIPTQDLTPTRPLPSALEEALGNHPFRTGDPGHPADDWEANTHSGKPSSSPTTERSFPPAPKTFCPSPQPPRGPGVSTISKPVMVHQEHNFVYRPAVKSEVLPQEMLKRMVVYQTAFRQDFRKYEEGFDPYSMFSPEQIAGKDVRLLRIKKEGSLDLALEGGVDSPVGKVVVSAVYEGGAAERHGGVVKGDEIMAINGKIVTDYTLAEAEAALQKAWNQGGDWIDLVVAVCPPKEYDDELRKPPAGPKAA

Target ORF information:

RefSeq Version	NM_001291182.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus USH1 protein network component harmonin (Ush1c), transcript variant b2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001291182.1

ORF Insert Sequence:

ATGGACCGGA AGGTGGCCCG AGAATTCCGA CACAAGGTGG ATTTTCTCAT TGAAAATGAC 
GCAGAGAAGG ACTATCTTTA TGATGTGCTG CGGATGTATC ACCAGACCAT GGATGTGGCT 
GTGCTCGTGG GAGACCTGAA GCTGGTCATC AATGAACCCA ACCGCCTGCC GCTGTTTGAT 
GCCATTCGAC CCCTGATCCC ACTGAAGCAC CAGGTGGAGT ATGACCAGCT GACACCCCGA 
CGCTCCAGGA AACTGAAGGA GGTACGCTTG GACCGTCTGC ACCCAGAAGG TCTCGGCCTC 
AGCGTGCGTG GAGGCCTGGA ATTTGGCTGT GGACTCTTTA TCTCCCACCT CATCAAAGGT 
GGCCAGGCAG ACAGCGTTGG GCTTCAGGTA GGGGATGAAA TTGTCCGGAT CAACGGCTAT 
TCCATCTCTT CCTGTACCCA TGAGGAAGTC ATCAACCTGA TCCGCACCAA GAAGACCGTG 
TCCATCAAAG TGAGACACAT CGGACTGATC CCTGTGAAGA GCTCTCCTGA GGAGTCCCTC 
AAATGGCAGT ATGTGGATCA GTTCGTGTCG GAATCTGGGG GTGTGCGAGG TGGCTTGGGC 
TCACCTGGCA ATCGGACAAC CAAGGAGAAG AAGGTGTTTA TCAGTCTAGT GGGCTCTCGG 
GGCCTGGGCT GCAGCATCTC CAGTGGCCCC ATCCAGAAGC CTGGCATCTT CGTCAGCCAC 
GTGAAGCCTG GCTCCCTGTC TGCAGAGGTG GGGTTAGAGA CAGGAGACCA GATTGTGGAA 
GTCAACGGCA TAGACTTCAC CAACCTGGAC CACAAGGAGG CTGTGAATGT CCTGAAGAGC 
AGCCGCAGCC TGACCATCTC CATCGTTGCT GGAGCCGGCC GGGAGCTGTT CATGACGGAC 
CGGGAACGGC TGGAGGAGGC ACGGCAGCGT GAGCTGCAAC GGCAGGAACT CCTCATGCAG 
AAGCGGCTGG CCATGGAGTC CAACAAGATC CTCCAGGAGC AGCAGGAGAT GGAGCGCCAG 
AGGAGAAAGG AGATCGCCCA GAAGGCTGCC GAGGAGAATG AGAGATACCG GAAGGAGATG 
GAACAGATCT CGGAGGAGGA AGAGAAGTTT AAGAAGCAGT GGAAAGAAGA CTGGGGTTCA 
AAGGAGCAGC TCATTCTGCC TAAGACCATC ACCGCAGAGG TGCACCCGGT GCCCCTTCGC 
AAGCCCAAGT CTTTTGGGTG GTTTTACCGT TACGATGGCA AATTCCCAAC CATCCGGAAG 
AAAGCAAAAG AGAAGAAGAA AGCCAAGTAC GATAGCCTGC AGGACCTGAG GAAGAACAAG 
AAGGAACTCG AGTTTGAACA AAAGCTCTAC AAAGAGAAGG AAGAGATGCT GGAGAAGGAG 
AAGCAGCTCA AGATCAACCG GCTGGCCCAG GAGGTGTCTG AGACTGAGCG GGAAGACCTT 
GAGGAATCGG AAAAGACTCA GTATTGGGTG GAGAGGCTCT GTCAGACGCG CCTCGAGCAG 
ATTTCCTCTG CAGAGAATGA GATTCCTGAG ATGACCACAG GGCCCCCACC TCCCCCACCT 
TCTGTGTCTC CTCTGGCCCC ACCCTTGAGA CGATTCGCTG GTGGGATCCA CCTCCACACC 
ACTGACCTGG ATGACATCCC CTTGGACATG TTCTACTACC CCCCCAAGAC TCCCTCAGCA 
CTGCCCGTGA TGCCCCACCC TCCATCTGTC AACCCACCTT CCAAGGTCCC CGCGCCCCCT 
GTGCTTCCCT CATCAGGTCA CGTGAGCAGC TCATCCTCAC CATGGGTGCA ACGCACTCCT 
CCCCCAATCC CCATTCCTCC GCCTCCATCC ATTCCCACCC AAGACCTCAC TCCTACCCGC 
CCATTGCCCT CAGCACTGGA AGAGGCCCTG GGCAACCACC CCTTCCGCAC TGGGGACCCA 
GGCCATCCAG CAGATGACTG GGAGGCAAAC ACCCACAGTG GGAAGCCCTC CAGCTCCCCC 
ACCACTGAGC GGAGCTTCCC ACCTGCCCCA AAGACATTCT GCCCAAGCCC ACAGCCTCCA 
CGAGGCCCTG GCGTGTCCAC CATCTCCAAA CCCGTCATGG TCCACCAGGA ACACAACTTC 
GTCTACAGGC CTGCTGTGAA GTCCGAGGTT CTGCCGCAGG AGATGCTGAA GAGGATGGTG 
GTATATCAGA CAGCATTCAG ACAAGATTTT CGAAAATACG AAGAAGGCTT TGATCCCTAC 
TCCATGTTCT CGCCAGAGCA GATCGCAGGG AAGGACGTCC GGCTTCTGCG AATCAAGAAG 
GAGGGGTCTT TAGACTTGGC CCTGGAGGGA GGCGTGGACT CCCCCGTGGG GAAGGTGGTC 
GTCTCTGCTG TTTATGAAGG GGGAGCTGCG GAGAGGCATG GTGGCGTTGT GAAAGGGGAT 
GAGATCATGG CCATCAATGG CAAGATTGTG ACGGACTACA CCCTGGCTGA GGCCGAGGCT 
GCCCTACAGA AGGCCTGGAA TCAGGGCGGG GACTGGATAG ACCTTGTGGT TGCTGTCTGT 
CCCCCTAAGG AGTATGATGA CGAGCTCCGA AAGCCCCCAG CTGGCCCGAA AGCAGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ush1c ( XM_006541212.3 ) cDNA ORF clone, Mus musculus(house mouse) -> XP_006541275.1 Mus musculus USH1 protein network component harmonin (Ush1c), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OMu39045
Clone ID Related Accession (Same CDS sequence)	XM_006541212.3
Accession Version	XM_006541212.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1554bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-07
Organism	Mus musculus(house mouse)
Product	harmonin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_000073.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 22, 2016 this sequence version replaced XM_006541212.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Mus musculus Annotation Release 108 Annotation Version :: 108 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.2 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATCACC AGACCATGGA TGTGGCTGTG CTCGTGGGAG ACCTGAAGCT GGTCATCAAT 
GAACCCAACC GCCTGCCGCT GTTTGATGCC ATTCGACCCC TGATCCCACT GAAGCACCAG 
GTGGAGTATG ACCAGCTGAC ACCCCGACGC TCCAGGAAAC TGAAGGAGGT ACGCTTGGAC 
CGTCTGCACC CAGAAGGTCT CGGCCTCAGC GTGCGTGGAG GCCTGGAATT TGGCTGTGGA 
CTCTTTATCT CCCACCTCAT CAAAGGTGGC CAGGCAGACA GCGTTGGGCT TCAGGTAGGG 
GATGAAATTG TCCGGATCAA CGGCTATTCC ATCTCTTCCT GTACCCATGA GGAAGTCATC 
AACCTGATCC GCACCAAGAA GACCGTGTCC ATCAAAGTGA GACACATCGG ACTGATCCCT 
GTGAAGAGCT CTCCTGAGGA GTCCCTCAAA TGGCAGTATG TGGATCAGTT CGTGTCGGAA 
TCTGGGGGTG TGCGAGGTGG CTTGGGCTCA CCTGGCAATC GGACAACCAA GGAGAAGAAG 
GTGTTTATCA GTCTAGTGGG CTCTCGGGGC CTGGGCTGCA GCATCTCCAG TGGCCCCATC 
CAGAAGCCTG GCATCTTCGT CAGCCACGTG AAGCCTGGCT CCCTGTCTGC AGAGGTGGGG 
TTAGAGACAG GAGACCAGAT TGTGGAAGTC AACGGCATAG ACTTCACCAA CCTGGACCAC 
AAGGAGGCTG TGAATGTCCT GAAGAGCAGC CGCAGCCTGA CCATCTCCAT CGTTGCTGGA 
GCCGGCCGGG AGCTGTTCAT GACGGACCGG GAACGGCTGG AGGAGGCACG GCAGCGTGAG 
CTGCAACGGC AGGAACTCCT CATGCAGAAG CGGCTGGCCA TGGAGTCCAA CAAGATCCTC 
CAGGAGCAGC AGGAGATGGA GCGCCAGAGG AGAAAGGAGA TCGCCCAGAA GGCTGCCGAG 
GAGAATGAGA GATACCGGAA GGAGATGGAA CAGATCTCGG AGGAGGAAGA GAAGTTTAAG 
AAGCAGTGGA AAGAAGACTG GGGTTCAAAG GAGCAGCTCA TTCTGCCTAA GACCATCACC 
GCAGAGGTGC ACCCGGTGCC CCTTCGCAAG CCCAAGTATG ATCAGGGAGT GGAGCCGGCA 
GACCACTTGG ATGGAAGCAC GGAGGAGCAG AGACAGCAGG ATTTTCGAAA ATACGAAGAA 
GGCTTTGATC CCTACTCCAT GTTCTCGCCA GAGCAGATCG CAGGGAAGGA CGTCCGGCTT 
CTGCGAATCA AGAAGGAGGG GTCTTTAGAC TTGGCCCTGG AGGGAGGCGT GGACTCCCCC 
GTGGGGAAGG TGGTCGTCTC TGCTGTTTAT GAAGGGGGAG CTGCGGAGAG GCATGGTGGC 
GTTGTGAAAG GGGATGAGAT CATGGCCATC AATGGCAAGA TTGTGACGGA CTACACCCTG 
GCTGAGGCCG AGGCTGCCCT ACAGAAGGCC TGGAATCAGG GCGGGGACTG GATAGACCTT 
GTGGTTGCTG TCTGTCCCCC TAAGGAGTAT GATGACGAGC TAACCTTCTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_006541275.1
CDS	118..1671
Translation	MYHQTMDVAVLVGDLKLVINEPNRLPLFDAIRPLIPLKHQVEYDQLTPRRSRKLKEVRLDRLHPEGLGLSVRGGLEFGCGLFISHLIKGGQADSVGLQVGDEIVRINGYSISSCTHEEVINLIRTKKTVSIKVRHIGLIPVKSSPEESLKWQYVDQFVSESGGVRGGLGSPGNRTTKEKKVFISLVGSRGLGCSISSGPIQKPGIFVSHVKPGSLSAEVGLETGDQIVEVNGIDFTNLDHKEAVNVLKSSRSLTISIVAGAGRELFMTDRERLEEARQRELQRQELLMQKRLAMESNKILQEQQEMERQRRKEIAQKAAEENERYRKEMEQISEEEEKFKKQWKEDWGSKEQLILPKTITAEVHPVPLRKPKYDQGVEPADHLDGSTEEQRQQDFRKYEEGFDPYSMFSPEQIAGKDVRLLRIKKEGSLDLALEGGVDSPVGKVVVSAVYEGGAAERHGGVVKGDEIMAINGKIVTDYTLAEAEAALQKAWNQGGDWIDLVVAVCPPKEYDDELTFF

Target ORF information:

RefSeq Version	XM_006541212.3
Organism	Mus musculus(house mouse)
Definition	Mus musculus USH1 protein network component harmonin (Ush1c), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_006541212.3

ORF Insert Sequence:

ATGTATCACC AGACCATGGA TGTGGCTGTG CTCGTGGGAG ACCTGAAGCT GGTCATCAAT 
GAACCCAACC GCCTGCCGCT GTTTGATGCC ATTCGACCCC TGATCCCACT GAAGCACCAG 
GTGGAGTATG ACCAGCTGAC ACCCCGACGC TCCAGGAAAC TGAAGGAGGT ACGCTTGGAC 
CGTCTGCACC CAGAAGGTCT CGGCCTCAGC GTGCGTGGAG GCCTGGAATT TGGCTGTGGA 
CTCTTTATCT CCCACCTCAT CAAAGGTGGC CAGGCAGACA GCGTTGGGCT TCAGGTAGGG 
GATGAAATTG TCCGGATCAA CGGCTATTCC ATCTCTTCCT GTACCCATGA GGAAGTCATC 
AACCTGATCC GCACCAAGAA GACCGTGTCC ATCAAAGTGA GACACATCGG ACTGATCCCT 
GTGAAGAGCT CTCCTGAGGA GTCCCTCAAA TGGCAGTATG TGGATCAGTT CGTGTCGGAA 
TCTGGGGGTG TGCGAGGTGG CTTGGGCTCA CCTGGCAATC GGACAACCAA GGAGAAGAAG 
GTGTTTATCA GTCTAGTGGG CTCTCGGGGC CTGGGCTGCA GCATCTCCAG TGGCCCCATC 
CAGAAGCCTG GCATCTTCGT CAGCCACGTG AAGCCTGGCT CCCTGTCTGC AGAGGTGGGG 
TTAGAGACAG GAGACCAGAT TGTGGAAGTC AACGGCATAG ACTTCACCAA CCTGGACCAC 
AAGGAGGCTG TGAATGTCCT GAAGAGCAGC CGCAGCCTGA CCATCTCCAT CGTTGCTGGA 
GCCGGCCGGG AGCTGTTCAT GACGGACCGG GAACGGCTGG AGGAGGCACG GCAGCGTGAG 
CTGCAACGGC AGGAACTCCT CATGCAGAAG CGGCTGGCCA TGGAGTCCAA CAAGATCCTC 
CAGGAGCAGC AGGAGATGGA GCGCCAGAGG AGAAAGGAGA TCGCCCAGAA GGCTGCCGAG 
GAGAATGAGA GATACCGGAA GGAGATGGAA CAGATCTCGG AGGAGGAAGA GAAGTTTAAG 
AAGCAGTGGA AAGAAGACTG GGGTTCAAAG GAGCAGCTCA TTCTGCCTAA GACCATCACC 
GCAGAGGTGC ACCCGGTGCC CCTTCGCAAG CCCAAGTATG ATCAGGGAGT GGAGCCGGCA 
GACCACTTGG ATGGAAGCAC GGAGGAGCAG AGACAGCAGG ATTTTCGAAA ATACGAAGAA 
GGCTTTGATC CCTACTCCAT GTTCTCGCCA GAGCAGATCG CAGGGAAGGA CGTCCGGCTT 
CTGCGAATCA AGAAGGAGGG GTCTTTAGAC TTGGCCCTGG AGGGAGGCGT GGACTCCCCC 
GTGGGGAAGG TGGTCGTCTC TGCTGTTTAT GAAGGGGGAG CTGCGGAGAG GCATGGTGGC 
GTTGTGAAAG GGGATGAGAT CATGGCCATC AATGGCAAGA TTGTGACGGA CTACACCCTG 
GCTGAGGCCG AGGCTGCCCT ACAGAAGGCC TGGAATCAGG GCGGGGACTG GATAGACCTT 
GTGGTTGCTG TCTGTCCCCC TAAGGAGTAT GATGACGAGC TAACCTTCTT TTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ush1c ( NM_153677.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_710143.2 Mus musculus USH1 protein network component harmonin (Ush1c), transcript variant b3, mRNA.

Price & Availability↑

CloneID	OMu15505
Clone ID Related Accession (Same CDS sequence)	NM_153677.2
Accession Version	NM_153677.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Mus musculus(house mouse)
Product	harmonin isoform b3
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF228925.1, BP775197.1, AC120135.7 and BY708209.1. On Jul 30, 2009 this sequence version replaced NM_153677.1. Transcript Variant: This variant (b3) encodes the longest isoform (b3). Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF228925.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849376 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCGGA AGGTGGCCCG AGAATTCCGA CACAAGGTGG ATTTTCTCAT TGAAAATGAC 
GCAGAGAAGG ACTATCTTTA TGATGTGCTG CGGATGTATC ACCAGACCAT GGATGTGGCT 
GTGCTCGTGG GAGACCTGAA GCTGGTCATC AATGAACCCA ACCGCCTGCC GCTGTTTGAT 
GCCATTCGAC CCCTGATCCC ACTGAAGCAC CAGGTGGAGT ATGACCAGCT GACACCCCGA 
CGCTCCAGGA AACTGAAGGA GGTACGCTTG GACCGTCTGC ACCCAGAAGG TCTCGGCCTC 
AGCGTGCGTG GAGGCCTGGA ATTTGGCTGT GGACTCTTTA TCTCCCACCT CATCAAAGGT 
GGCCAGGCAG ACAGCGTTGG GCTTCAGGTA GGGGATGAAA TTGTCCGGAT CAACGGCTAT 
TCCATCTCTT CCTGTACCCA TGAGGAAGTC ATCAACCTGA TCCGCACCAA GAAGACCGTG 
TCCATCAAAG TGAGACACAT CGGACTGATC CCTGTGAAGA GCTCTCCTGA GGAGTCCCTC 
AAATGGCAGT ATGTGGATCA GTTCGTGTCG GAATCTGGGG GTGTGCGAGG TGGCTTGGGC 
TCACCTGGCA ATCGGACAAC CAAGGAGAAG AAGGTGTTTA TCAGTCTAGT GGGCTCTCGG 
GGCCTGGGCT GCAGCATCTC CAGTGGCCCC ATCCAGAAGC CTGGCATCTT CGTCAGCCAC 
GTGAAGCCTG GCTCCCTGTC TGCAGAGGTG GGGTTAGAGA CAGGAGACCA GATTGTGGAA 
GTCAACGGCA TAGACTTCAC CAACCTGGAC CACAAGGAGG CTGTGAATGT CCTGAAGAGC 
AGCCGCAGCC TGACCATCTC CATCGTTGCT GGAGCCGGCC GGGAGCTGTT CATGACGGAC 
CGGGAACGGC TGGAGGAGGC ACGGCAGCGT GAGCTGCAAC GGCAGGAACT CCTCATGCAG 
AAGCGGCTGG CCATGGAGTC CAACAAGATC CTCCAGGAGC AGCAGGAGAT GGAGCGCCAG 
AGGAGAAAGG AGATCGCCCA GAAGGCTGCC GAGGAGAATG AGAGATACCG GAAGGAGATG 
GAACAGATCT CGGAGGAGGA AGAGAAGTTT AAGAAGCAGT GGAAAGAAGA CTGGGGTTCA 
AAGGAGCAGC TCATTCTGCC TAAGACCATC ACCGCAGAGG TGCACCCGGT GCCCCTTCGC 
AAGCCCAAGT CTTTTGGGTG GTTTTACCGT TACGATGGCA AATTCCCAAC CATCCGGAAG 
AAAGCAAAAG AGAAGAAGAA AGCCAAGTAC GATAGCCTGC AGGACCTGAG GAAGAACAAG 
AAGGAACTCG AGTTTGAACA AAAGCTCTAC AAAGAGAAGG AAGAGATGCT GGAGAAGGAG 
AAGCAGCTCA AGATCAACCG GCTGGCCCAG GAGGTGTCTG AGACTGAGCG GGAAGACCTT 
GAGGAATCGG AAAAGACTCA GTATTGGGTG GAGAGGCTCT GTCAGACGCG CCTCGAGCAG 
ATTTCCTCTG CAGAGAATGA GATTCCTGAG ATGACCACAG GGCCCCCACC TCCCCCACCT 
TCTGTGTCTC CTCTGGCCCC ACCCTTGAGA CGATTCGCTG GTGGGATCCA CCTCCACACC 
ACTGACCTGG ATGACATCCC CTTGGACATG TTCTACTACC CCCCCAAGAC TCCCTCAGCA 
CTGCCCGTGA TGCCCCACCC TCCATCTGTC AACCCACCTT CCAAGGTCCC CGCGCCCCCT 
GTGCTTCCCT CATCAGGTCA CGTGAGCAGC TCATCCTCAC CATGGGTGCA ACGCACTCCT 
CCCCCAATCC CCATTCCTCC GCCTCCATCC ATTCCCACCC AAGACCTCAC TCCTACCCGC 
CCATTGCCCT CAGCACTGGA AGAGGCCCTG GGCAACCACC CCTTCCGCAC TGGGGACCCA 
GGCCATCCAG CAGATGACTG GGAGGCAAAC ACCCACAGTG GGAAGCCCTC CAGCTCCCCC 
ACCACTGAGC GGAGCTTCCC ACCTGCCCCA AAGACATTCT GCCCAAGCCC ACAGCCTCCA 
CGAGGCCCTG GCGTGTCCAC CATCTCCAAA CCCGTCATGG TCCACCAGGA ACACAACTTC 
GTCTACAGGC CTGCTGTGAA GTCCGAGGTT CTGCCGCAGG AGATGCTGAA GAGGATGGTG 
GTATATCAGA CAGCATTCAG ACAAGATTTT CGAAAATACG AAGAAGGCTT TGATCCCTAC 
TCCATGTTCT CGCCAGAGCA GATCGCAGGG AAGGACGTCC GGCTTCTGCG AATCAAGAAG 
GAGGGGTCTT TAGACTTGGC CCTGGAGGGA GGCGTGGACT CCCCCGTGGG GAAGGTGGTC 
GTCTCTGCTG TTTATGAAGG GGGAGCTGCG GAGAGGCATG GTGGCGTTGT GAAAGGGGAT 
GAGATCATGG CCATCAATGG CAAGATTGTG ACGGACTACA CCCTGGCTGA GGCCGAGGCT 
GCCCTACAGA AGGCCTGGAA TCAGGGCGGG GACTGGATAG ACCTTGTGGT TGCTGTCTGT 
CCCCCTAAGG AGTATGATGA CGAGCTCTCT TCTCTTCCCT CCTCTGCAGC CGAAAGCCCC 
CAGCTGGCCC GAAAGCAGCT TGAAGCCTAT GAGCCCGTGT GTAGACACGG GTTTTTCCTG 
CAGCTGGAGC CCACGAACCT TCTTTTAAAG TCCAGAGAGA GAAACCAGAC AGACCCATCC 
TGGAGGCCGG CGAGCTCCGC ACCCAGCCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_710143.2
CDS	27..2759
Translation	MDRKVAREFRHKVDFLIENDAEKDYLYDVLRMYHQTMDVAVLVGDLKLVINEPNRLPLFDAIRPLIPLKHQVEYDQLTPRRSRKLKEVRLDRLHPEGLGLSVRGGLEFGCGLFISHLIKGGQADSVGLQVGDEIVRINGYSISSCTHEEVINLIRTKKTVSIKVRHIGLIPVKSSPEESLKWQYVDQFVSESGGVRGGLGSPGNRTTKEKKVFISLVGSRGLGCSISSGPIQKPGIFVSHVKPGSLSAEVGLETGDQIVEVNGIDFTNLDHKEAVNVLKSSRSLTISIVAGAGRELFMTDRERLEEARQRELQRQELLMQKRLAMESNKILQEQQEMERQRRKEIAQKAAEENERYRKEMEQISEEEEKFKKQWKEDWGSKEQLILPKTITAEVHPVPLRKPKSFGWFYRYDGKFPTIRKKAKEKKKAKYDSLQDLRKNKKELEFEQKLYKEKEEMLEKEKQLKINRLAQEVSETEREDLEESEKTQYWVERLCQTRLEQISSAENEIPEMTTGPPPPPPSVSPLAPPLRRFAGGIHLHTTDLDDIPLDMFYYPPKTPSALPVMPHPPSVNPPSKVPAPPVLPSSGHVSSSSSPWVQRTPPPIPIPPPPSIPTQDLTPTRPLPSALEEALGNHPFRTGDPGHPADDWEANTHSGKPSSSPTTERSFPPAPKTFCPSPQPPRGPGVSTISKPVMVHQEHNFVYRPAVKSEVLPQEMLKRMVVYQTAFRQDFRKYEEGFDPYSMFSPEQIAGKDVRLLRIKKEGSLDLALEGGVDSPVGKVVVSAVYEGGAAERHGGVVKGDEIMAINGKIVTDYTLAEAEAALQKAWNQGGDWIDLVVAVCPPKEYDDELSSLPSSAAESPQLARKQLEAYEPVCRHGFFLQLEPTNLLLKSRERNQTDPSWRPASSAPSP

Target ORF information:

RefSeq Version	NM_153677.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus USH1 protein network component harmonin (Ush1c), transcript variant b3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_153677.2

ORF Insert Sequence:

ATGGACCGGA AGGTGGCCCG AGAATTCCGA CACAAGGTGG ATTTTCTCAT TGAAAATGAC 
GCAGAGAAGG ACTATCTTTA TGATGTGCTG CGGATGTATC ACCAGACCAT GGATGTGGCT 
GTGCTCGTGG GAGACCTGAA GCTGGTCATC AATGAACCCA ACCGCCTGCC GCTGTTTGAT 
GCCATTCGAC CCCTGATCCC ACTGAAGCAC CAGGTGGAGT ATGACCAGCT GACACCCCGA 
CGCTCCAGGA AACTGAAGGA GGTACGCTTG GACCGTCTGC ACCCAGAAGG TCTCGGCCTC 
AGCGTGCGTG GAGGCCTGGA ATTTGGCTGT GGACTCTTTA TCTCCCACCT CATCAAAGGT 
GGCCAGGCAG ACAGCGTTGG GCTTCAGGTA GGGGATGAAA TTGTCCGGAT CAACGGCTAT 
TCCATCTCTT CCTGTACCCA TGAGGAAGTC ATCAACCTGA TCCGCACCAA GAAGACCGTG 
TCCATCAAAG TGAGACACAT CGGACTGATC CCTGTGAAGA GCTCTCCTGA GGAGTCCCTC 
AAATGGCAGT ATGTGGATCA GTTCGTGTCG GAATCTGGGG GTGTGCGAGG TGGCTTGGGC 
TCACCTGGCA ATCGGACAAC CAAGGAGAAG AAGGTGTTTA TCAGTCTAGT GGGCTCTCGG 
GGCCTGGGCT GCAGCATCTC CAGTGGCCCC ATCCAGAAGC CTGGCATCTT CGTCAGCCAC 
GTGAAGCCTG GCTCCCTGTC TGCAGAGGTG GGGTTAGAGA CAGGAGACCA GATTGTGGAA 
GTCAACGGCA TAGACTTCAC CAACCTGGAC CACAAGGAGG CTGTGAATGT CCTGAAGAGC 
AGCCGCAGCC TGACCATCTC CATCGTTGCT GGAGCCGGCC GGGAGCTGTT CATGACGGAC 
CGGGAACGGC TGGAGGAGGC ACGGCAGCGT GAGCTGCAAC GGCAGGAACT CCTCATGCAG 
AAGCGGCTGG CCATGGAGTC CAACAAGATC CTCCAGGAGC AGCAGGAGAT GGAGCGCCAG 
AGGAGAAAGG AGATCGCCCA GAAGGCTGCC GAGGAGAATG AGAGATACCG GAAGGAGATG 
GAACAGATCT CGGAGGAGGA AGAGAAGTTT AAGAAGCAGT GGAAAGAAGA CTGGGGTTCA 
AAGGAGCAGC TCATTCTGCC TAAGACCATC ACCGCAGAGG TGCACCCGGT GCCCCTTCGC 
AAGCCCAAGT CTTTTGGGTG GTTTTACCGT TACGATGGCA AATTCCCAAC CATCCGGAAG 
AAAGCAAAAG AGAAGAAGAA AGCCAAGTAC GATAGCCTGC AGGACCTGAG GAAGAACAAG 
AAGGAACTCG AGTTTGAACA AAAGCTCTAC AAAGAGAAGG AAGAGATGCT GGAGAAGGAG 
AAGCAGCTCA AGATCAACCG GCTGGCCCAG GAGGTGTCTG AGACTGAGCG GGAAGACCTT 
GAGGAATCGG AAAAGACTCA GTATTGGGTG GAGAGGCTCT GTCAGACGCG CCTCGAGCAG 
ATTTCCTCTG CAGAGAATGA GATTCCTGAG ATGACCACAG GGCCCCCACC TCCCCCACCT 
TCTGTGTCTC CTCTGGCCCC ACCCTTGAGA CGATTCGCTG GTGGGATCCA CCTCCACACC 
ACTGACCTGG ATGACATCCC CTTGGACATG TTCTACTACC CCCCCAAGAC TCCCTCAGCA 
CTGCCCGTGA TGCCCCACCC TCCATCTGTC AACCCACCTT CCAAGGTCCC CGCGCCCCCT 
GTGCTTCCCT CATCAGGTCA CGTGAGCAGC TCATCCTCAC CATGGGTGCA ACGCACTCCT 
CCCCCAATCC CCATTCCTCC GCCTCCATCC ATTCCCACCC AAGACCTCAC TCCTACCCGC 
CCATTGCCCT CAGCACTGGA AGAGGCCCTG GGCAACCACC CCTTCCGCAC TGGGGACCCA 
GGCCATCCAG CAGATGACTG GGAGGCAAAC ACCCACAGTG GGAAGCCCTC CAGCTCCCCC 
ACCACTGAGC GGAGCTTCCC ACCTGCCCCA AAGACATTCT GCCCAAGCCC ACAGCCTCCA 
CGAGGCCCTG GCGTGTCCAC CATCTCCAAA CCCGTCATGG TCCACCAGGA ACACAACTTC 
GTCTACAGGC CTGCTGTGAA GTCCGAGGTT CTGCCGCAGG AGATGCTGAA GAGGATGGTG 
GTATATCAGA CAGCATTCAG ACAAGATTTT CGAAAATACG AAGAAGGCTT TGATCCCTAC 
TCCATGTTCT CGCCAGAGCA GATCGCAGGG AAGGACGTCC GGCTTCTGCG AATCAAGAAG 
GAGGGGTCTT TAGACTTGGC CCTGGAGGGA GGCGTGGACT CCCCCGTGGG GAAGGTGGTC 
GTCTCTGCTG TTTATGAAGG GGGAGCTGCG GAGAGGCATG GTGGCGTTGT GAAAGGGGAT 
GAGATCATGG CCATCAATGG CAAGATTGTG ACGGACTACA CCCTGGCTGA GGCCGAGGCT 
GCCCTACAGA AGGCCTGGAA TCAGGGCGGG GACTGGATAG ACCTTGTGGT TGCTGTCTGT 
CCCCCTAAGG AGTATGATGA CGAGCTCTCT TCTCTTCCCT CCTCTGCAGC CGAAAGCCCC 
CAGCTGGCCC GAAAGCAGCT TGAAGCCTAT GAGCCCGTGT GTAGACACGG GTTTTTCCTG 
CAGCTGGAGC CCACGAACCT TCTTTTAAAG TCCAGAGAGA GAAACCAGAC AGACCCATCC 
TGGAGGCCGG CGAGCTCCGC ACCCAGCCCC TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ush1c ( NM_001163733.1 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_001157205.1 Mus musculus USH1 protein network component harmonin (Ush1c), transcript variant b4, mRNA.

Price & Availability↑

CloneID	OMu15515
Clone ID Related Accession (Same CDS sequence)	NM_001163733.1
Accession Version	NM_001163733.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2676bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-22
Organism	Mus musculus(house mouse)
Product	harmonin isoform b4
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF228925.1, BP775197.1, AY246556.1, AC120135.7 and BY708209.1. Transcript Variant: This variant (b4) has an additional segment in the 3' coding region, resulting in frame-shift, as compared to variant b3. The resulting isoform (b4) is shorter and has a different C-terminus, as compared to isoform b3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AY246556.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMN00849374, SAMN00849376 [ECO:0000350] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCGGA AGGTGGCCCG AGAATTCCGA CACAAGGTGG ATTTTCTCAT TGAAAATGAC 
GCAGAGAAGG ACTATCTTTA TGATGTGCTG CGGATGTATC ACCAGACCAT GGATGTGGCT 
GTGCTCGTGG GAGACCTGAA GCTGGTCATC AATGAACCCA ACCGCCTGCC GCTGTTTGAT 
GCCATTCGAC CCCTGATCCC ACTGAAGCAC CAGGTGGAGT ATGACCAGCT GACACCCCGA 
CGCTCCAGGA AACTGAAGGA GGTACGCTTG GACCGTCTGC ACCCAGAAGG TCTCGGCCTC 
AGCGTGCGTG GAGGCCTGGA ATTTGGCTGT GGACTCTTTA TCTCCCACCT CATCAAAGGT 
GGCCAGGCAG ACAGCGTTGG GCTTCAGGTA GGGGATGAAA TTGTCCGGAT CAACGGCTAT 
TCCATCTCTT CCTGTACCCA TGAGGAAGTC ATCAACCTGA TCCGCACCAA GAAGACCGTG 
TCCATCAAAG TGAGACACAT CGGACTGATC CCTGTGAAGA GCTCTCCTGA GGAGTCCCTC 
AAATGGCAGT ATGTGGATCA GTTCGTGTCG GAATCTGGGG GTGTGCGAGG TGGCTTGGGC 
TCACCTGGCA ATCGGACAAC CAAGGAGAAG AAGGTGTTTA TCAGTCTAGT GGGCTCTCGG 
GGCCTGGGCT GCAGCATCTC CAGTGGCCCC ATCCAGAAGC CTGGCATCTT CGTCAGCCAC 
GTGAAGCCTG GCTCCCTGTC TGCAGAGGTG GGGTTAGAGA CAGGAGACCA GATTGTGGAA 
GTCAACGGCA TAGACTTCAC CAACCTGGAC CACAAGGAGG CTGTGAATGT CCTGAAGAGC 
AGCCGCAGCC TGACCATCTC CATCGTTGCT GGAGCCGGCC GGGAGCTGTT CATGACGGAC 
CGGGAACGGC TGGAGGAGGC ACGGCAGCGT GAGCTGCAAC GGCAGGAACT CCTCATGCAG 
AAGCGGCTGG CCATGGAGTC CAACAAGATC CTCCAGGAGC AGCAGGAGAT GGAGCGCCAG 
AGGAGAAAGG AGATCGCCCA GAAGGCTGCC GAGGAGAATG AGAGATACCG GAAGGAGATG 
GAACAGATCT CGGAGGAGGA AGAGAAGTTT AAGAAGCAGT GGAAAGAAGA CTGGGGTTCA 
AAGGAGCAGC TCATTCTGCC TAAGACCATC ACCGCAGAGG TGCACCCGGT GCCCCTTCGC 
AAGCCCAAGT CTTTTGGGTG GTTTTACCGT TACGATGGCA AATTCCCAAC CATCCGGAAG 
AAAGCAAAAG AGAAGAAGAA AGCCAAGTAC GATAGCCTGC AGGACCTGAG GAAGAACAAG 
AAGGAACTCG AGTTTGAACA AAAGCTCTAC AAAGAGAAGG AAGAGATGCT GGAGAAGGAG 
AAGCAGCTCA AGATCAACCG GCTGGCCCAG GAGGTGTCTG AGACTGAGCG GGAAGACCTT 
GAGGAATCGG AAAAGACTCA GTATTGGGTG GAGAGGCTCT GTCAGACGCG CCTCGAGCAG 
ATTTCCTCTG CAGAGAATGA GATTCCTGAG ATGACCACAG GGCCCCCACC TCCCCCACCT 
TCTGTGTCTC CTCTGGCCCC ACCCTTGAGA CGATTCGCTG GTGGGATCCA CCTCCACACC 
ACTGACCTGG ATGACATCCC CTTGGACATG TTCTACTACC CCCCCAAGAC TCCCTCAGCA 
CTGCCCGTGA TGCCCCACCC TCCATCTGTC AACCCACCTT CCAAGGTCCC CGCGCCCCCT 
GTGCTTCCCT CATCAGGTCA CGTGAGCAGC TCATCCTCAC CATGGGTGCA ACGCACTCCT 
CCCCCAATCC CCATTCCTCC GCCTCCATCC ATTCCCACCC AAGACCTCAC TCCTACCCGC 
CCATTGCCCT CAGCACTGGA AGAGGCCCTG GGCAACCACC CCTTCCGCAC TGGGGACCCA 
GGCCATCCAG CAGATGACTG GGAGGCAAAC ACCCACAGTG GGAAGCCCTC CAGCTCCCCC 
ACCACTGAGC GGAGCTTCCC ACCTGCCCCA AAGACATTCT GCCCAAGCCC ACAGCCTCCA 
CGAGGCCCTG GCGTGTCCAC CATCTCCAAA CCCGTCATGG TCCACCAGGA ACACAACTTC 
GTCTACAGGC CTGCTGTGAA GTCCGAGGTT CTGCCGCAGG AGATGCTGAA GAGGATGGTG 
GTATATCAGA CAGCATTCAG ACAAGATTTT CGAAAATACG AAGAAGGCTT TGATCCCTAC 
TCCATGTTCT CGCCAGAGCA GATCGCAGGG AAGGACGTCC GGCTTCTGCG AATCAAGAAG 
GAGGGGTCTT TAGACTTGGC CCTGGAGGGA GGCGTGGACT CCCCCGTGGG GAAGGTGGTC 
GTCTCTGCTG TTTATGAAGG GGGAGCTGCG GAGAGGCATG GTGGCGTTGT GAAAGGGGAT 
GAGATCATGG CCATCAATGG CAAGATTGTG ACGGACTACA CCCTGGCTGA GGCCGAGGCT 
GCCCTACAGA AGGCCTGGAA TCAGGGCGGG GACTGGATAG ACCTTGTGGT TGCTGTCTGT 
CCCCCTAAGG AGTATGATGA CGAGCTCTCT TCTCTTCCCT CCTCTGCAGC CGAAAGCCCC 
CAGCTGGCCC GAAAGCAGCT TGAAGCCTAT GAGCCCGTGT GTAGACACGG GTTTTTCCTG 
CAGCTGGAGC CCACGGTGAC TAGAACCTTC TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001157205.1
CDS	27..2702
Translation	MDRKVAREFRHKVDFLIENDAEKDYLYDVLRMYHQTMDVAVLVGDLKLVINEPNRLPLFDAIRPLIPLKHQVEYDQLTPRRSRKLKEVRLDRLHPEGLGLSVRGGLEFGCGLFISHLIKGGQADSVGLQVGDEIVRINGYSISSCTHEEVINLIRTKKTVSIKVRHIGLIPVKSSPEESLKWQYVDQFVSESGGVRGGLGSPGNRTTKEKKVFISLVGSRGLGCSISSGPIQKPGIFVSHVKPGSLSAEVGLETGDQIVEVNGIDFTNLDHKEAVNVLKSSRSLTISIVAGAGRELFMTDRERLEEARQRELQRQELLMQKRLAMESNKILQEQQEMERQRRKEIAQKAAEENERYRKEMEQISEEEEKFKKQWKEDWGSKEQLILPKTITAEVHPVPLRKPKSFGWFYRYDGKFPTIRKKAKEKKKAKYDSLQDLRKNKKELEFEQKLYKEKEEMLEKEKQLKINRLAQEVSETEREDLEESEKTQYWVERLCQTRLEQISSAENEIPEMTTGPPPPPPSVSPLAPPLRRFAGGIHLHTTDLDDIPLDMFYYPPKTPSALPVMPHPPSVNPPSKVPAPPVLPSSGHVSSSSSPWVQRTPPPIPIPPPPSIPTQDLTPTRPLPSALEEALGNHPFRTGDPGHPADDWEANTHSGKPSSSPTTERSFPPAPKTFCPSPQPPRGPGVSTISKPVMVHQEHNFVYRPAVKSEVLPQEMLKRMVVYQTAFRQDFRKYEEGFDPYSMFSPEQIAGKDVRLLRIKKEGSLDLALEGGVDSPVGKVVVSAVYEGGAAERHGGVVKGDEIMAINGKIVTDYTLAEAEAALQKAWNQGGDWIDLVVAVCPPKEYDDELSSLPSSAAESPQLARKQLEAYEPVCRHGFFLQLEPTVTRTFF

Target ORF information:

RefSeq Version	NM_001163733.1
Organism	Mus musculus(house mouse)
Definition	Mus musculus USH1 protein network component harmonin (Ush1c), transcript variant b4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001163733.1

ORF Insert Sequence:

ATGGACCGGA AGGTGGCCCG AGAATTCCGA CACAAGGTGG ATTTTCTCAT TGAAAATGAC 
GCAGAGAAGG ACTATCTTTA TGATGTGCTG CGGATGTATC ACCAGACCAT GGATGTGGCT 
GTGCTCGTGG GAGACCTGAA GCTGGTCATC AATGAACCCA ACCGCCTGCC GCTGTTTGAT 
GCCATTCGAC CCCTGATCCC ACTGAAGCAC CAGGTGGAGT ATGACCAGCT GACACCCCGA 
CGCTCCAGGA AACTGAAGGA GGTACGCTTG GACCGTCTGC ACCCAGAAGG TCTCGGCCTC 
AGCGTGCGTG GAGGCCTGGA ATTTGGCTGT GGACTCTTTA TCTCCCACCT CATCAAAGGT 
GGCCAGGCAG ACAGCGTTGG GCTTCAGGTA GGGGATGAAA TTGTCCGGAT CAACGGCTAT 
TCCATCTCTT CCTGTACCCA TGAGGAAGTC ATCAACCTGA TCCGCACCAA GAAGACCGTG 
TCCATCAAAG TGAGACACAT CGGACTGATC CCTGTGAAGA GCTCTCCTGA GGAGTCCCTC 
AAATGGCAGT ATGTGGATCA GTTCGTGTCG GAATCTGGGG GTGTGCGAGG TGGCTTGGGC 
TCACCTGGCA ATCGGACAAC CAAGGAGAAG AAGGTGTTTA TCAGTCTAGT GGGCTCTCGG 
GGCCTGGGCT GCAGCATCTC CAGTGGCCCC ATCCAGAAGC CTGGCATCTT CGTCAGCCAC 
GTGAAGCCTG GCTCCCTGTC TGCAGAGGTG GGGTTAGAGA CAGGAGACCA GATTGTGGAA 
GTCAACGGCA TAGACTTCAC CAACCTGGAC CACAAGGAGG CTGTGAATGT CCTGAAGAGC 
AGCCGCAGCC TGACCATCTC CATCGTTGCT GGAGCCGGCC GGGAGCTGTT CATGACGGAC 
CGGGAACGGC TGGAGGAGGC ACGGCAGCGT GAGCTGCAAC GGCAGGAACT CCTCATGCAG 
AAGCGGCTGG CCATGGAGTC CAACAAGATC CTCCAGGAGC AGCAGGAGAT GGAGCGCCAG 
AGGAGAAAGG AGATCGCCCA GAAGGCTGCC GAGGAGAATG AGAGATACCG GAAGGAGATG 
GAACAGATCT CGGAGGAGGA AGAGAAGTTT AAGAAGCAGT GGAAAGAAGA CTGGGGTTCA 
AAGGAGCAGC TCATTCTGCC TAAGACCATC ACCGCAGAGG TGCACCCGGT GCCCCTTCGC 
AAGCCCAAGT CTTTTGGGTG GTTTTACCGT TACGATGGCA AATTCCCAAC CATCCGGAAG 
AAAGCAAAAG AGAAGAAGAA AGCCAAGTAC GATAGCCTGC AGGACCTGAG GAAGAACAAG 
AAGGAACTCG AGTTTGAACA AAAGCTCTAC AAAGAGAAGG AAGAGATGCT GGAGAAGGAG 
AAGCAGCTCA AGATCAACCG GCTGGCCCAG GAGGTGTCTG AGACTGAGCG GGAAGACCTT 
GAGGAATCGG AAAAGACTCA GTATTGGGTG GAGAGGCTCT GTCAGACGCG CCTCGAGCAG 
ATTTCCTCTG CAGAGAATGA GATTCCTGAG ATGACCACAG GGCCCCCACC TCCCCCACCT 
TCTGTGTCTC CTCTGGCCCC ACCCTTGAGA CGATTCGCTG GTGGGATCCA CCTCCACACC 
ACTGACCTGG ATGACATCCC CTTGGACATG TTCTACTACC CCCCCAAGAC TCCCTCAGCA 
CTGCCCGTGA TGCCCCACCC TCCATCTGTC AACCCACCTT CCAAGGTCCC CGCGCCCCCT 
GTGCTTCCCT CATCAGGTCA CGTGAGCAGC TCATCCTCAC CATGGGTGCA ACGCACTCCT 
CCCCCAATCC CCATTCCTCC GCCTCCATCC ATTCCCACCC AAGACCTCAC TCCTACCCGC 
CCATTGCCCT CAGCACTGGA AGAGGCCCTG GGCAACCACC CCTTCCGCAC TGGGGACCCA 
GGCCATCCAG CAGATGACTG GGAGGCAAAC ACCCACAGTG GGAAGCCCTC CAGCTCCCCC 
ACCACTGAGC GGAGCTTCCC ACCTGCCCCA AAGACATTCT GCCCAAGCCC ACAGCCTCCA 
CGAGGCCCTG GCGTGTCCAC CATCTCCAAA CCCGTCATGG TCCACCAGGA ACACAACTTC 
GTCTACAGGC CTGCTGTGAA GTCCGAGGTT CTGCCGCAGG AGATGCTGAA GAGGATGGTG 
GTATATCAGA CAGCATTCAG ACAAGATTTT CGAAAATACG AAGAAGGCTT TGATCCCTAC 
TCCATGTTCT CGCCAGAGCA GATCGCAGGG AAGGACGTCC GGCTTCTGCG AATCAAGAAG 
GAGGGGTCTT TAGACTTGGC CCTGGAGGGA GGCGTGGACT CCCCCGTGGG GAAGGTGGTC 
GTCTCTGCTG TTTATGAAGG GGGAGCTGCG GAGAGGCATG GTGGCGTTGT GAAAGGGGAT 
GAGATCATGG CCATCAATGG CAAGATTGTG ACGGACTACA CCCTGGCTGA GGCCGAGGCT 
GCCCTACAGA AGGCCTGGAA TCAGGGCGGG GACTGGATAG ACCTTGTGGT TGCTGTCTGT 
CCCCCTAAGG AGTATGATGA CGAGCTCTCT TCTCTTCCCT CCTCTGCAGC CGAAAGCCCC 
CAGCTGGCCC GAAAGCAGCT TGAAGCCTAT GAGCCCGTGT GTAGACACGG GTTTTTCCTG 
CAGCTGGAGC CCACGGTGAC TAGAACCTTC TTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Ush1c ( NM_023649.2 ) cDNA ORF clone, Mus musculus(house mouse) -> NP_076138.2 Mus musculus USH1 protein network component harmonin (Ush1c), transcript variant a1, mRNA.

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CloneID	OMu15513
Clone ID Related Accession (Same CDS sequence)	NM_023649.2
Accession Version	NM_023649.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1647bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-02-21
Organism	Mus musculus(house mouse)
Product	harmonin isoform a1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from AF228924.1, BP775197.1 and BY708209.1. On Jul 30, 2009 this sequence version replaced NM_023649.1. Transcript Variant: This variant (a1) differs from variant b3 in the middle coding region and in the 3' coding region. The resulting isoform (a1) is much shorter, and it lacks an internal segment and has a different C-terminus, as compared to isoform b3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on transcript alignments. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF228924.1, BC010819.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN00849380, SAMN00849389 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGGACCGGA AGGTGGCCCG AGAATTCCGA CACAAGGTGG ATTTTCTCAT TGAAAATGAC 
GCAGAGAAGG ACTATCTTTA TGATGTGCTG CGGATGTATC ACCAGACCAT GGATGTGGCT 
GTGCTCGTGG GAGACCTGAA GCTGGTCATC AATGAACCCA ACCGCCTGCC GCTGTTTGAT 
GCCATTCGAC CCCTGATCCC ACTGAAGCAC CAGGTGGAGT ATGACCAGCT GACACCCCGA 
CGCTCCAGGA AACTGAAGGA GGTACGCTTG GACCGTCTGC ACCCAGAAGG TCTCGGCCTC 
AGCGTGCGTG GAGGCCTGGA ATTTGGCTGT GGACTCTTTA TCTCCCACCT CATCAAAGGT 
GGCCAGGCAG ACAGCGTTGG GCTTCAGGTA GGGGATGAAA TTGTCCGGAT CAACGGCTAT 
TCCATCTCTT CCTGTACCCA TGAGGAAGTC ATCAACCTGA TCCGCACCAA GAAGACCGTG 
TCCATCAAAG TGAGACACAT CGGACTGATC CCTGTGAAGA GCTCTCCTGA GGAGTCCCTC 
AAATGGCAGT ATGTGGATCA GTTCGTGTCG GAATCTGGGG GTGTGCGAGG TGGCTTGGGC 
TCACCTGGCA ATCGGACAAC CAAGGAGAAG AAGGTGTTTA TCAGTCTAGT GGGCTCTCGG 
GGCCTGGGCT GCAGCATCTC CAGTGGCCCC ATCCAGAAGC CTGGCATCTT CGTCAGCCAC 
GTGAAGCCTG GCTCCCTGTC TGCAGAGGTG GGGTTAGAGA CAGGAGACCA GATTGTGGAA 
GTCAACGGCA TAGACTTCAC CAACCTGGAC CACAAGGAGG CTGTGAATGT CCTGAAGAGC 
AGCCGCAGCC TGACCATCTC CATCGTTGCT GGAGCCGGCC GGGAGCTGTT CATGACGGAC 
CGGGAACGGC TGGAGGAGGC ACGGCAGCGT GAGCTGCAAC GGCAGGAACT CCTCATGCAG 
AAGCGGCTGG CCATGGAGTC CAACAAGATC CTCCAGGAGC AGCAGGAGAT GGAGCGCCAG 
AGGAGAAAGG AGATCGCCCA GAAGGCTGCC GAGGAGAATG AGAGATACCG GAAGGAGATG 
GAACAGATCT CGGAGGAGGA AGAGAAGTTT AAGAAGCAGT GGAAAGAAGA CTGGGGTTCA 
AAGGAGCAGC TCATTCTGCC TAAGACCATC ACCGCAGAGG TGCACCCGGT GCCCCTTCGC 
AAGCCCAAGT ATGATCAGGG AGTGGAGCCG GCAGACCACT TGGATGGAAG CACGGAGGAG 
CAGAGACAGC AGGATTTTCG AAAATACGAA GAAGGCTTTG ATCCCTACTC CATGTTCTCG 
CCAGAGCAGA TCGCAGGGAA GGACGTCCGG CTTCTGCGAA TCAAGAAGGA GGGGTCTTTA 
GACTTGGCCC TGGAGGGAGG CGTGGACTCC CCCGTGGGGA AGGTGGTCGT CTCTGCTGTT 
TATGAAGGGG GAGCTGCGGA GAGGCATGGT GGCGTTGTGA AAGGGGATGA GATCATGGCC 
ATCAATGGCA AGATTGTGAC GGACTACACC CTGGCTGAGG CCGAGGCTGC CCTACAGAAG 
GCCTGGAATC AGGGCGGGGA CTGGATAGAC CTTGTGGTTG CTGTCTGTCC CCCTAAGGAG 
TATGATGACG AGCTAACCTT CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_076138.2
CDS	27..1673
Translation	MDRKVAREFRHKVDFLIENDAEKDYLYDVLRMYHQTMDVAVLVGDLKLVINEPNRLPLFDAIRPLIPLKHQVEYDQLTPRRSRKLKEVRLDRLHPEGLGLSVRGGLEFGCGLFISHLIKGGQADSVGLQVGDEIVRINGYSISSCTHEEVINLIRTKKTVSIKVRHIGLIPVKSSPEESLKWQYVDQFVSESGGVRGGLGSPGNRTTKEKKVFISLVGSRGLGCSISSGPIQKPGIFVSHVKPGSLSAEVGLETGDQIVEVNGIDFTNLDHKEAVNVLKSSRSLTISIVAGAGRELFMTDRERLEEARQRELQRQELLMQKRLAMESNKILQEQQEMERQRRKEIAQKAAEENERYRKEMEQISEEEEKFKKQWKEDWGSKEQLILPKTITAEVHPVPLRKPKYDQGVEPADHLDGSTEEQRQQDFRKYEEGFDPYSMFSPEQIAGKDVRLLRIKKEGSLDLALEGGVDSPVGKVVVSAVYEGGAAERHGGVVKGDEIMAINGKIVTDYTLAEAEAALQKAWNQGGDWIDLVVAVCPPKEYDDELTFF

Target ORF information:

RefSeq Version	NM_023649.2
Organism	Mus musculus(house mouse)
Definition	Mus musculus USH1 protein network component harmonin (Ush1c), transcript variant a1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_023649.2

ORF Insert Sequence:

ATGGACCGGA AGGTGGCCCG AGAATTCCGA CACAAGGTGG ATTTTCTCAT TGAAAATGAC 
GCAGAGAAGG ACTATCTTTA TGATGTGCTG CGGATGTATC ACCAGACCAT GGATGTGGCT 
GTGCTCGTGG GAGACCTGAA GCTGGTCATC AATGAACCCA ACCGCCTGCC GCTGTTTGAT 
GCCATTCGAC CCCTGATCCC ACTGAAGCAC CAGGTGGAGT ATGACCAGCT GACACCCCGA 
CGCTCCAGGA AACTGAAGGA GGTACGCTTG GACCGTCTGC ACCCAGAAGG TCTCGGCCTC 
AGCGTGCGTG GAGGCCTGGA ATTTGGCTGT GGACTCTTTA TCTCCCACCT CATCAAAGGT 
GGCCAGGCAG ACAGCGTTGG GCTTCAGGTA GGGGATGAAA TTGTCCGGAT CAACGGCTAT 
TCCATCTCTT CCTGTACCCA TGAGGAAGTC ATCAACCTGA TCCGCACCAA GAAGACCGTG 
TCCATCAAAG TGAGACACAT CGGACTGATC CCTGTGAAGA GCTCTCCTGA GGAGTCCCTC 
AAATGGCAGT ATGTGGATCA GTTCGTGTCG GAATCTGGGG GTGTGCGAGG TGGCTTGGGC 
TCACCTGGCA ATCGGACAAC CAAGGAGAAG AAGGTGTTTA TCAGTCTAGT GGGCTCTCGG 
GGCCTGGGCT GCAGCATCTC CAGTGGCCCC ATCCAGAAGC CTGGCATCTT CGTCAGCCAC 
GTGAAGCCTG GCTCCCTGTC TGCAGAGGTG GGGTTAGAGA CAGGAGACCA GATTGTGGAA 
GTCAACGGCA TAGACTTCAC CAACCTGGAC CACAAGGAGG CTGTGAATGT CCTGAAGAGC 
AGCCGCAGCC TGACCATCTC CATCGTTGCT GGAGCCGGCC GGGAGCTGTT CATGACGGAC 
CGGGAACGGC TGGAGGAGGC ACGGCAGCGT GAGCTGCAAC GGCAGGAACT CCTCATGCAG 
AAGCGGCTGG CCATGGAGTC CAACAAGATC CTCCAGGAGC AGCAGGAGAT GGAGCGCCAG 
AGGAGAAAGG AGATCGCCCA GAAGGCTGCC GAGGAGAATG AGAGATACCG GAAGGAGATG 
GAACAGATCT CGGAGGAGGA AGAGAAGTTT AAGAAGCAGT GGAAAGAAGA CTGGGGTTCA 
AAGGAGCAGC TCATTCTGCC TAAGACCATC ACCGCAGAGG TGCACCCGGT GCCCCTTCGC 
AAGCCCAAGT ATGATCAGGG AGTGGAGCCG GCAGACCACT TGGATGGAAG CACGGAGGAG 
CAGAGACAGC AGGATTTTCG AAAATACGAA GAAGGCTTTG ATCCCTACTC CATGTTCTCG 
CCAGAGCAGA TCGCAGGGAA GGACGTCCGG CTTCTGCGAA TCAAGAAGGA GGGGTCTTTA 
GACTTGGCCC TGGAGGGAGG CGTGGACTCC CCCGTGGGGA AGGTGGTCGT CTCTGCTGTT 
TATGAAGGGG GAGCTGCGGA GAGGCATGGT GGCGTTGTGA AAGGGGATGA GATCATGGCC 
ATCAATGGCA AGATTGTGAC GGACTACACC CTGGCTGAGG CCGAGGCTGC CCTACAGAAG 
GCCTGGAATC AGGGCGGGGA CTGGATAGAC CTTGTGGTTG CTGTCTGTCC CCCTAAGGAG 
TATGATGACG AGCTAACCTT CTTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Antisense oligonucleotide therapy rescues disruptions in organization of exploratory movements associated with Usher syndrome type 1C in mice.
Behavioural brain research33876-87(2018 Feb)
Donaldson TN,Jennings KT,Cherep LA,McNeela AM,Depreux FF,Jodelka FM,Hastings ML,Wallace DG

Rescue of peripheral vestibular function in Usher syndrome mice using a splice-switching antisense oligonucleotide.
Human molecular genetics26(18)3482-3494(2017 Sep)
Vijayakumar S,Depreux FF,Jodelka FM,Lentz JJ,Rigo F,Jones TA,Hastings ML

The roles of USH1 proteins and PDZ domain-containing USH proteins in USH2 complex integrity in cochlear hair cells.
Human molecular genetics26(3)624-636(2017 02)
Zou J,Chen Q,Almishaal A,Mathur PD,Zheng T,Tian C,Zheng QY,Yang J

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Ush1c cDNA ORF clone, Mus musculus(house mouse)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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