FAM65B cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	FAM65B
Entrez Gene ID	563800
Full Name	RHO family interacting cell polarization regulator 2
Synonyms	fam65b,si:dkey-218n20.1
General protein information	Preferred Names RHO family interacting cell polarization regulator 2 Names rho family-interacting cell polarization regulator 2 protein FAM65B family with sequence similarity 65, member B
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021468090.1	XP_021323765.1	protein FAM65B isoform X2
XM_021468091.1	XP_021323766.1	protein FAM65B isoform X4
XM_005159634.3	XP_005159691.1	protein FAM65B isoform X1
NM_001045026.1	NP_001038491.1	rho family-interacting cell polarization regulator 2
XM_005159634.4	XP_005159691.1	protein FAM65B isoform X1
XM_009294297.3	XP_009292572.1	protein FAM65B isoform X3
XM_009294297.2	XP_009292572.1	protein FAM65B isoform X2

Pathways from BioSystems

Homologies

Bos taurus (cattle)	FAM65B	NP_001030502.1
Homo sapiens (human)	FAM65B	NP_055537.2
Gallus gallus (chicken)	FAM65B	NP_001012883.1
Danio rerio (zebrafish)	fam65b	NP_001038491.1
Canis lupus familiaris (dog)	FAM65B	XP_005640143.1
Rattus norvegicus (Norway rat)	Fam65b	NP_001014031.2
	LOC715354	XP_001105528.2
Xenopus tropicalis (tropical clawed frog)	fam65b	NP_001123748.1
Pan troglodytes (chimpanzee)	FAM65B	XP_001171972.1
Mus musculus (house mouse)	Fam65b	NP_083955.1

Gene Ontology
Bibliography

Related articles in PubMed

Fam65b is important for formation of the HDAC6-dysferlin protein complex during myogenic cell differentiation.
Balasubramanian A, Kawahara G, Gupta VA, Rozkalne A, Beauvais A, Kunkel LM, Gussoni E
FASEB journal : official publication of the Federation of American Societies for Experimental Biology28(7)2955-69(2014 Jul)

FAM65B is a membrane-associated protein of hair cell stereocilia required for hearing.
Diaz-Horta O, Subasioglu-Uzak A, Grati M, DeSmidt A, Foster J 2nd, Cao L, Bademci G, Tokgoz-Yilmaz S, Duman D, Cengiz FB, Abad C, Mittal R, Blanton S, Liu XZ, Farooq A, Walz K, Lu Z, Tekin M
Proceedings of the National Academy of Sciences of the United States of America111(27)9864-8(2014 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following FAM65B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the FAM65B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa44191	XM_021468091.1 Latest version!	Danio rerio family with sequence similarity 65, member B (fam65b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$510.30 $729.00
ODa150097	XM_021468090.1 Latest version!	Danio rerio family with sequence similarity 65, member B (fam65b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa04084	NM_001045026.1 Latest version!	Danio rerio RHO family interacting cell polarization regulator 2 (ripor2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa24292	XM_005159634.4 Latest version!	Danio rerio family with sequence similarity 65, member B (fam65b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa44190	XM_009294297.3 Latest version!	Danio rerio family with sequence similarity 65, member B (fam65b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa24292	XM_005159634.3	Danio rerio family with sequence similarity 65, member B (fam65b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa44190	XM_009294297.2	Danio rerio family with sequence similarity 65, member B (fam65b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

FAM65B ( XM_021468091.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021323766.1 Danio rerio family with sequence similarity 65, member B (fam65b), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa44191
Clone ID Related Accession (Same CDS sequence)	XM_021468091.1
Accession Version	XM_021468091.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3000bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein FAM65B isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTGCTG GCACTCACTC TCCTGGAGGA CCCAATGGCA TCATCAGGAG CCAGTCGTTC 
GCTGGCTTCA GCACCCTGCA GGAGAGACGC TCACGGTGCA ACTCCTTCAT GGGGAACTCG 
GCAGTGCAGA AGAAGCCCAT CTCCAAGCCC AGGAAGCCCC ATCTGTCCGG GCATAAATCC 
AGCAGCAGCA GCAGCAGCTC ACGAGAGCCA CAGCCTAAAC GTGTAGAGGA GGTTTACAGC 
GCACTCAAGC AAGGCCTGGA TGAATATCTG GAGGTGCACC AAACCGAGCT GGATAAACTC 
ACTTCCCTCA TGAAGGACAT GAAGAGGAAC TCCAGACTGG GTGTGCTGTA TGATCTTGAC 
AAGCAAATCA AGACCATAGA GCGATACATG CGACGACTGG AGTTTCACAT CAGCAAGGTC 
GATGAGCTGT ACGAGGGCTT TTGCATTCAG AGGAGGTTGC GTGATGGCGC CAGTAAGATG 
AAACAGGCCT TCACTGCTTC GCCCTCCACC AAAGGCACAC GTGAGAGCCT GGCGGAGGTC 
AATCGCAGAT ACAAAGAATA TACAGAGAAC ATGAGCACTT TTGAGGGCGA GCTGGAGAAC 
CTGTTGGGGG AGTTTCATAT CAAAATGAAA GGATTGGCTG GTTTTGCCAG ACTTTGTCCT 
GGTGATCAGT ATGAGATTTT CATGAGATAC GGCCGACAGC GGTGGAAACT GAAGGGGAAG 
ATCGAAGGAA ACTCTAAGCA GAGTTGGGAT GGAGAGGAGA TGATCTTCAT GCCCCTTATT 
TCAGACCTTA TTAACATCAA GGTGACCGAG TTAAAGGGTT TGGCCACTCA TGTTTTGGTG 
GGCAGTGTGA TCTGTGAAAC CAAGGACCTG TTCACCGCTA TGCCTCAGGT GGTTGCTGTA 
GACGTCAATG ACCTGGGAAC CATTAAACTT AACCTGGAGG TCGCCTGGTT TCCGTTTGAC 
GTGGAGGATC TGACTCTGTC CTCTGGGAAC GTGAGTAAAG CCACTGCCCT TCAGAGACGG 
GTTTCAGTCT ATAGCCAAGG CACACCCGAG ACGCCCACTT TCCAGGACAC ATCTTTCTTT 
TCCACTTTAC CAGATGACGT CTTTGAGAAT GGAGGCTGCG GTGTGGCTGA ATGCAAGCGT 
CTGTCCTTCA CCTTCTCAGA CACGTCCACC TCAAGCCCCG TCCCTGTCCA GTCAAACCCT 
GAGATTACAG TTACCCCTCC CGAGACGGAC CCATCATCTG AACCACCTCC AACCTCAGAG 
GAGCAGCTGG TTGAGGAGGA GTGTGTAGAA GAAGACTGCG GCAGTATCAG CGTCAGTGTC 
AGCGTCAGTG ATCCGGACCT CGAGTGGGAC CAGGTTGAAA CCCAGGGGCC TGAAACAGGC 
TCTGTGGCCC TCTCGTTGTG CTCTGAGAGT CAGCTTTCTG CAGTAGGACC AGAGGACGTG 
GTGTTTCTGG AGCCCAATGT GTCTGATGAA GCCCTGGAGC TGAAGCCGGT GGAGCTGGAT 
GGGGAGGAAG GCAGTCTGAC CAGGCAGCTG GTGAGGAGGC TGACCTCGTC TGATATGCTG 
CCTGAGACCA GTGCTCTGAG CTGGGCTGGA GAGAACAGCA GGACTTTTAT GGAGAGCAGT 
CTGGAGGAGG TCATTCAGTC GCTGCTCCTG CGGCTAGAGA GTTTAGGGCA GCGCTGCGGG 
GAACTGCAGG ACCTGGAGCA GGAAGTCATG AGACTTGAAG ACCTGCTCAA GTGTCGAGTG 
CATGCCCATA GGAGCCGCTC ATCAAGCCTC AGTTTGACGG TAGAAAGCGC TCTGGAAAGT 
TTTGACTTCC TCAACACCTC TGACTTCGAT GACGATGACA CAGGAGATGA CGCTGGCACT 
CTCTCACGTG CTGTCTTCTT TGACATGGAG TCAGAGAGAA TGGGGTCAGG TCAACATCCA 
GAAGCGCGAG GCCATCTGAG TGAAGCCCTC ACAGAGGACA CAGGCGTGGG GAACAGTGTG 
GCCGGCAGCC CTTTGCCCCT CACGACAGGA AACGAGAACC TAGACGTAGC CGTCGTCATT 
CATCTGCAAT ACTGCAATCA TCTCATTCAG TTGTTGACTT CAGGAGGAAG CCAGTTACAG 
CATAAGACAT ACCTGCACAA ACTAGCAACT CAAACTCTGC TGTTAGAGGA GCTCAGTGAG 
AGGAACTTTG ACAGACCGAG CAGCTGCACT TCAGTGCCTG ATGTGCTGCC CGGGCTGGTG 
GAGCGGCCGG CGCTGATGTC TCTATGGTCC GACTGCAGCG GTGGGCTTTT CCACACCACA 
CTAGACCGAG TATTAAAGCA CATGCATCTC AGCTTTGCCT TAGGACTGCA ACAGGTTCTG 
CCTCACTCTC CTGACTCAGT GATCAAGATG GTGGTGAATG AGATGATGGA CAGGAGTGAA 
CTGGCCTCCT CCTCAACTTC TCCAGCACAG GACGTCATCA CTGTGTTTCA GTTCCACCGC 
TACATTGTTG AACACTGTAT CACAGACATG GAGCAACACC TGCTGGATTT AGCCAAAGAG 
GTCATGCTTG AAGAAGCCCT GAGCTGTGGA GATCCAGAAC GCTCTCTGAA GGAACTAAAG 
AAAGTGTCCA TCACGTGTCT TCAGCCCCGT TCTCAAACTC TATTGGCTGT GGCTGGTCTG 
CTGACCACTG ATGACCCTGA AGTGATGAAG GCCACCACAG ACTTCCTCTC CTCTGCTGCC 
TCACATCAGC CCTTCAGGTC AAAGGCTGTT GATTGTTACA CACAAGCCTT ATCAGAAAGT 
GAGTTACACA CACAGAGAGC TGCATGTGCT GCTCTGAGCT GCCTTCAGGC TGTGGAGAGC 
ATTGATGCTG TCATTTTACT GTGTGACTCT GTGGATGAAG AGCTCCGGCA GATTGCTATT 
GAAACCCTCC TCACTTTCGG TGAGGATGGG CGGCTGGCAT ACGAGCAACT GGACACCGTC 
CCGAGGGAGA TGGTGCGGCT GGGCACTCGG CGTGGAAATG CGGTGACCAC TTCATTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323766.1
CDS	243..3242
Translation	MAAGTHSPGGPNGIIRSQSFAGFSTLQERRSRCNSFMGNSAVQKKPISKPRKPHLSGHKSSSSSSSSREPQPKRVEEVYSALKQGLDEYLEVHQTELDKLTSLMKDMKRNSRLGVLYDLDKQIKTIERYMRRLEFHISKVDELYEGFCIQRRLRDGASKMKQAFTASPSTKGTRESLAEVNRRYKEYTENMSTFEGELENLLGEFHIKMKGLAGFARLCPGDQYEIFMRYGRQRWKLKGKIEGNSKQSWDGEEMIFMPLISDLINIKVTELKGLATHVLVGSVICETKDLFTAMPQVVAVDVNDLGTIKLNLEVAWFPFDVEDLTLSSGNVSKATALQRRVSVYSQGTPETPTFQDTSFFSTLPDDVFENGGCGVAECKRLSFTFSDTSTSSPVPVQSNPEITVTPPETDPSSEPPPTSEEQLVEEECVEEDCGSISVSVSVSDPDLEWDQVETQGPETGSVALSLCSESQLSAVGPEDVVFLEPNVSDEALELKPVELDGEEGSLTRQLVRRLTSSDMLPETSALSWAGENSRTFMESSLEEVIQSLLLRLESLGQRCGELQDLEQEVMRLEDLLKCRVHAHRSRSSSLSLTVESALESFDFLNTSDFDDDDTGDDAGTLSRAVFFDMESERMGSGQHPEARGHLSEALTEDTGVGNSVAGSPLPLTTGNENLDVAVVIHLQYCNHLIQLLTSGGSQLQHKTYLHKLATQTLLLEELSERNFDRPSSCTSVPDVLPGLVERPALMSLWSDCSGGLFHTTLDRVLKHMHLSFALGLQQVLPHSPDSVIKMVVNEMMDRSELASSSTSPAQDVITVFQFHRYIVEHCITDMEQHLLDLAKEVMLEEALSCGDPERSLKELKKVSITCLQPRSQTLLAVAGLLTTDDPEVMKATTDFLSSAASHQPFRSKAVDCYTQALSESELHTQRAACAALSCLQAVESIDAVILLCDSVDEELRQIAIETLLTFGEDGRLAYEQLDTVPREMVRLGTRRGNAVTTSF

Target ORF information:

RefSeq Version	XM_021468091.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio family with sequence similarity 65, member B (fam65b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468091.1

ORF Insert Sequence:

ATGGCTGCTG GCACTCACTC TCCTGGAGGA CCCAATGGCA TCATCAGGAG CCAGTCGTTC 
GCTGGCTTCA GCACCCTGCA GGAGAGACGC TCACGGTGCA ACTCCTTCAT GGGGAACTCG 
GCAGTGCAGA AGAAGCCCAT CTCCAAGCCC AGGAAGCCCC ATCTGTCCGG GCATAAATCC 
AGCAGCAGCA GCAGCAGCTC ACGAGAGCCA CAGCCTAAAC GTGTAGAGGA GGTTTACAGC 
GCACTCAAGC AAGGCCTGGA TGAATATCTG GAGGTGCACC AAACCGAGCT GGATAAACTC 
ACTTCCCTCA TGAAGGACAT GAAGAGGAAC TCCAGACTGG GTGTGCTGTA TGATCTTGAC 
AAGCAAATCA AGACCATAGA GCGATACATG CGACGACTGG AGTTTCACAT CAGCAAGGTC 
GATGAGCTGT ACGAGGGCTT TTGCATTCAG AGGAGGTTGC GTGATGGCGC CAGTAAGATG 
AAACAGGCCT TCACTGCTTC GCCCTCCACC AAAGGCACAC GTGAGAGCCT GGCGGAGGTC 
AATCGCAGAT ACAAAGAATA TACAGAGAAC ATGAGCACTT TTGAGGGCGA GCTGGAGAAC 
CTGTTGGGGG AGTTTCATAT CAAAATGAAA GGATTGGCTG GTTTTGCCAG ACTTTGTCCT 
GGTGATCAGT ATGAGATTTT CATGAGATAC GGCCGACAGC GGTGGAAACT GAAGGGGAAG 
ATCGAAGGAA ACTCTAAGCA GAGTTGGGAT GGAGAGGAGA TGATCTTCAT GCCCCTTATT 
TCAGACCTTA TTAACATCAA GGTGACCGAG TTAAAGGGTT TGGCCACTCA TGTTTTGGTG 
GGCAGTGTGA TCTGTGAAAC CAAGGACCTG TTCACCGCTA TGCCTCAGGT GGTTGCTGTA 
GACGTCAATG ACCTGGGAAC CATTAAACTT AACCTGGAGG TCGCCTGGTT TCCGTTTGAC 
GTGGAGGATC TGACTCTGTC CTCTGGGAAC GTGAGTAAAG CCACTGCCCT TCAGAGACGG 
GTTTCAGTCT ATAGCCAAGG CACACCCGAG ACGCCCACTT TCCAGGACAC ATCTTTCTTT 
TCCACTTTAC CAGATGACGT CTTTGAGAAT GGAGGCTGCG GTGTGGCTGA ATGCAAGCGT 
CTGTCCTTCA CCTTCTCAGA CACGTCCACC TCAAGCCCCG TCCCTGTCCA GTCAAACCCT 
GAGATTACAG TTACCCCTCC CGAGACGGAC CCATCATCTG AACCACCTCC AACCTCAGAG 
GAGCAGCTGG TTGAGGAGGA GTGTGTAGAA GAAGACTGCG GCAGTATCAG CGTCAGTGTC 
AGCGTCAGTG ATCCGGACCT CGAGTGGGAC CAGGTTGAAA CCCAGGGGCC TGAAACAGGC 
TCTGTGGCCC TCTCGTTGTG CTCTGAGAGT CAGCTTTCTG CAGTAGGACC AGAGGACGTG 
GTGTTTCTGG AGCCCAATGT GTCTGATGAA GCCCTGGAGC TGAAGCCGGT GGAGCTGGAT 
GGGGAGGAAG GCAGTCTGAC CAGGCAGCTG GTGAGGAGGC TGACCTCGTC TGATATGCTG 
CCTGAGACCA GTGCTCTGAG CTGGGCTGGA GAGAACAGCA GGACTTTTAT GGAGAGCAGT 
CTGGAGGAGG TCATTCAGTC GCTGCTCCTG CGGCTAGAGA GTTTAGGGCA GCGCTGCGGG 
GAACTGCAGG ACCTGGAGCA GGAAGTCATG AGACTTGAAG ACCTGCTCAA GTGTCGAGTG 
CATGCCCATA GGAGCCGCTC ATCAAGCCTC AGTTTGACGG TAGAAAGCGC TCTGGAAAGT 
TTTGACTTCC TCAACACCTC TGACTTCGAT GACGATGACA CAGGAGATGA CGCTGGCACT 
CTCTCACGTG CTGTCTTCTT TGACATGGAG TCAGAGAGAA TGGGGTCAGG TCAACATCCA 
GAAGCGCGAG GCCATCTGAG TGAAGCCCTC ACAGAGGACA CAGGCGTGGG GAACAGTGTG 
GCCGGCAGCC CTTTGCCCCT CACGACAGGA AACGAGAACC TAGACGTAGC CGTCGTCATT 
CATCTGCAAT ACTGCAATCA TCTCATTCAG TTGTTGACTT CAGGAGGAAG CCAGTTACAG 
CATAAGACAT ACCTGCACAA ACTAGCAACT CAAACTCTGC TGTTAGAGGA GCTCAGTGAG 
AGGAACTTTG ACAGACCGAG CAGCTGCACT TCAGTGCCTG ATGTGCTGCC CGGGCTGGTG 
GAGCGGCCGG CGCTGATGTC TCTATGGTCC GACTGCAGCG GTGGGCTTTT CCACACCACA 
CTAGACCGAG TATTAAAGCA CATGCATCTC AGCTTTGCCT TAGGACTGCA ACAGGTTCTG 
CCTCACTCTC CTGACTCAGT GATCAAGATG GTGGTGAATG AGATGATGGA CAGGAGTGAA 
CTGGCCTCCT CCTCAACTTC TCCAGCACAG GACGTCATCA CTGTGTTTCA GTTCCACCGC 
TACATTGTTG AACACTGTAT CACAGACATG GAGCAACACC TGCTGGATTT AGCCAAAGAG 
GTCATGCTTG AAGAAGCCCT GAGCTGTGGA GATCCAGAAC GCTCTCTGAA GGAACTAAAG 
AAAGTGTCCA TCACGTGTCT TCAGCCCCGT TCTCAAACTC TATTGGCTGT GGCTGGTCTG 
CTGACCACTG ATGACCCTGA AGTGATGAAG GCCACCACAG ACTTCCTCTC CTCTGCTGCC 
TCACATCAGC CCTTCAGGTC AAAGGCTGTT GATTGTTACA CACAAGCCTT ATCAGAAAGT 
GAGTTACACA CACAGAGAGC TGCATGTGCT GCTCTGAGCT GCCTTCAGGC TGTGGAGAGC 
ATTGATGCTG TCATTTTACT GTGTGACTCT GTGGATGAAG AGCTCCGGCA GATTGCTATT 
GAAACCCTCC TCACTTTCGG TGAGGATGGG CGGCTGGCAT ACGAGCAACT GGACACCGTC 
CCGAGGGAGA TGGTGCGGCT GGGCACTCGG CGTGGAAATG CGGTGACCAC TTCATTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FAM65B ( XM_021468090.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021323765.1 Danio rerio family with sequence similarity 65, member B (fam65b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa150097
Clone ID Related Accession (Same CDS sequence)	XM_021468090.1
Accession Version	XM_021468090.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein FAM65B isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAACTGT CACGAAAGCT CCAATGGATC CGCGAGCGGC CCTGGAGGGC CCGGGCCCGT 
CACAGGAAGT TCAACAGAGT GTCCTCACGC TTCCCAGACA TCATGGCTGC TGGCACTCAC 
TCTCCTGGAG GACCCAATGG CATCATCAGG AGCCAGTCGT TCGCTGGCTT CAGCACCCTG 
CAGGAGAGAC GCTCACGGTG CAACTCCTTC ATGGGGAACT CGGCAGTGCA GAAGAAGCCC 
ATCTCCAAGC CCAGGAAGCC CCATCTGTCC GGGCATAAAT CCAGCAGCAG CAGCAGCAGC 
TCACGAGAGC CACAGCCTAA ACGTGTAGAG GAGGTTTACA GCGCACTCAA GCAAGGCCTG 
GATGAATATC TGGAGGTGCA CCAAACCGAG CTGGATAAAC TCACTTCCCT CATGAAGGAC 
ATGAAGAGGA ACTCCAGACT GGGTGTGCTG TATGATCTTG ACAAGCAAAT CAAGACCATA 
GAGCGATACA TGCGACGACT GGAGTTTCAC ATCAGCAAGG TCGATGAGCT GTACGAGGGC 
TTTTGCATTC AGAGGAGGTT GCGTGATGGC GCCAGTAAGA TGAAACAGGC CTTCACTGCT 
TCGCCCTCCA CCAAAGGCAC ACGTGAGAGC CTGGCGGAGG TCAATCGCAG ATACAAAGAA 
TATACAGAGA ACATGAGCAC TTTTGAGGGC GAGCTGGAGA ACCTGTTGGG GGAGTTTCAT 
ATCAAAATGA AAGGATTGGC TGGTTTTGCC AGACTTTGTC CTGGTGATCA GTATGAGATT 
TTCATGAGAT ACGGCCGACA GCGGTGGAAA CTGAAGGGGA AGATCGAAGG AAACTCTAAG 
CAGAGTTGGG ATGGAGAGGA GATGATCTTC ATGCCCCTTA TTTCAGACCT TATTAACATC 
AAGGTGACCG AGTTAAAGGG TTTGGCCACT CATGTTTTGG TGGGCAGTGT GATCTGTGAA 
ACCAAGGACC TGTTCACCGC TATGCCTCAG GTGGTTGCTG TAGACGTCAA TGACCTGGGA 
ACCATTAAAC TTAACCTGGA GGTCGCCTGG TTTCCGTTTG ACGTGGAGGA TCTGACTCTG 
TCCTCTGGGA ACGTGAGTAA AGCCACTGCC CTTCAGAGAC GGGTTTCAGT CTATAGCCAA 
GGCACACCCG AGACGCCCAC TTTCCAGGAC ACATCTTTCT TTTCCACTTT ACCAGATGAC 
GTCTTTGAGA ATGGAGGCTG CGGTGTGGCT GAATGCAAGC GTCTGTCCTT CACCTTCTCA 
GACACGTCCA CCTCAAGCCC CGTCCCTGTC CAGTCAAACC CTGAGATTAC AGTTACCCCT 
CCCGAGACGG ACCCATCATC TGAACCACCT CCAACCTCAG AGGAGCAGCT GGTTGAGGAG 
GAGTGTGTAG AAGAAGACTG CGGCAGTATC AGCGTCAGTG TCAGCGTCAG TGATCCGGAC 
CTCGAGTGGG ACCAGGTTGA AACCCAGGGG CCTGAAACAG GCTCTGTGGC CCTCTCGTTG 
TGCTCTGAGA GTCAGCTTTC TGCAGTAGGA CCAGAGGACG TGGTGTTTCT GGAGCCCAAT 
GTGTCTGATG AAGCCCTGGA GCTGAAGCCG GTGGAGCTGG ATGGGGAGGA AGGCAGTCTG 
ACCAGGCAGC TGGTGAGGAG GCTGACCTCG TCTGATATGC TGCCTGAGAC CAGTGCTCTG 
AGCTGGGCTG GAGAGAACAG CAGGACTTTT ATGGAGAGCA GTCTGGAGGA GGTCATTCAG 
TCGCTGCTCC TGCGGCTAGA GAGTTTAGGG CAGCGCTGCG GGGAACTGCA GGACCTGGAG 
CAGGAAGTCA TGAGACTTGA AGACCTGCTC AAGTGTCGAG TGCATGCCCA TAGGAGCCGC 
TCATCAAGCC TCAGTTTGAC GGTAGAAAGC GCTCTGGAAA GTTTTGACTT CCTCAACACC 
TCTGACTTCG ATGACGATGA CACAGGAGAT GACGCTGGCA CTCTCTCACG TGCTGTCTTC 
TTTGACATGG AGTCAGAGAG AATGGGGTCA GGTCAACATC CAGAAGCGCG AGGCCATCTG 
AGTGAAGCCC TCACAGAGGA CACAGGCGTG GGGAACAGTG TGGCCGGCAG CCCTTTGCCC 
CTCACGACAG GAAACGAGAA CCTAGACGTA GCCGTCGTCA TTCATCTGCA ATACTGCAAT 
CATCTCATTC AGTTGTTGAC TTCAGGAGGA AGCCAGTTAC AGCATAAGAC ATACCTGCAC 
AAACTAGCAA CTCAAACTCT GCTGTTAGAG GAGCTCAGTG AGAGGAACTT TGACAGACCG 
AGCAGCTGCA CTTCAGTGCC TGATGTGCTG CCCGGGCTGG TGGAGCGGCC GGCGCTGATG 
TCTCTATGGT CCGACTGCAG CGGTGGGCTT TTCCACACCA CACTAGACCG AGTATTAAAG 
CACATGCATC TCAGCTTTGC CTTAGGACTG CAACAGGTTC TGCCTCACTC TCCTGACTCA 
GTGATCAAGA TGGTGGTGAA TGAGATGATG GACAGGAGTG AACTGGCCTC CTCCTCAACT 
TCTCCAGCAC AGGACGTCAT CACTGTGTTT CAGTTCCACC GCTACATTGT TGAACACTGT 
ATCACAGACA TGGAGCAACA CCTGCTGGAT TTAGCCAAAG AGGTCATGCT TGAAGAAGCC 
CTGAGCTGTG GAGATCCAGA ACGCTCTCTG AAGGAACTAA AGAAAGTGTC CATCACGTGT 
CTTCAGCCCC GTTCTCAAAC TCTATTGGCT GTGGCTGGTC TGCTGACCAC TGATGACCCT 
GAAGTGATGA AGGCCACCAC AGACTTCCTC TCCTCTGCTG CCTCACATCA GCCCTTCAGG 
TCAAAGGCTG TTGATTGTTA CACACAAGCC TTATCAGAAA GTGAGTTACA CACACAGAGA 
GCTGCATGTG CTGCTCTGAG CTGCCTTCAG GCTGTGGAGA GCATTGATGC TGTCATTTTA 
CTGTGTGACT CTGTGGATGA AGAGCTCCGG CAGATTGCTA TTGAAACCCT CCTCACTTTC 
GGTGAGGATG GGCGGCTGGC ATACGAGCAA CTGGACACCG TCCCGAGGGA GATGGTGCGG 
CTGGGCACTC GGCGTGGAAA TGCGGTGACC ACTTCATTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021323765.1
CDS	233..3334
Translation	MELSRKLQWIRERPWRARARHRKFNRVSSRFPDIMAAGTHSPGGPNGIIRSQSFAGFSTLQERRSRCNSFMGNSAVQKKPISKPRKPHLSGHKSSSSSSSSREPQPKRVEEVYSALKQGLDEYLEVHQTELDKLTSLMKDMKRNSRLGVLYDLDKQIKTIERYMRRLEFHISKVDELYEGFCIQRRLRDGASKMKQAFTASPSTKGTRESLAEVNRRYKEYTENMSTFEGELENLLGEFHIKMKGLAGFARLCPGDQYEIFMRYGRQRWKLKGKIEGNSKQSWDGEEMIFMPLISDLINIKVTELKGLATHVLVGSVICETKDLFTAMPQVVAVDVNDLGTIKLNLEVAWFPFDVEDLTLSSGNVSKATALQRRVSVYSQGTPETPTFQDTSFFSTLPDDVFENGGCGVAECKRLSFTFSDTSTSSPVPVQSNPEITVTPPETDPSSEPPPTSEEQLVEEECVEEDCGSISVSVSVSDPDLEWDQVETQGPETGSVALSLCSESQLSAVGPEDVVFLEPNVSDEALELKPVELDGEEGSLTRQLVRRLTSSDMLPETSALSWAGENSRTFMESSLEEVIQSLLLRLESLGQRCGELQDLEQEVMRLEDLLKCRVHAHRSRSSSLSLTVESALESFDFLNTSDFDDDDTGDDAGTLSRAVFFDMESERMGSGQHPEARGHLSEALTEDTGVGNSVAGSPLPLTTGNENLDVAVVIHLQYCNHLIQLLTSGGSQLQHKTYLHKLATQTLLLEELSERNFDRPSSCTSVPDVLPGLVERPALMSLWSDCSGGLFHTTLDRVLKHMHLSFALGLQQVLPHSPDSVIKMVVNEMMDRSELASSSTSPAQDVITVFQFHRYIVEHCITDMEQHLLDLAKEVMLEEALSCGDPERSLKELKKVSITCLQPRSQTLLAVAGLLTTDDPEVMKATTDFLSSAASHQPFRSKAVDCYTQALSESELHTQRAACAALSCLQAVESIDAVILLCDSVDEELRQIAIETLLTFGEDGRLAYEQLDTVPREMVRLGTRRGNAVTTSF

Target ORF information:

RefSeq Version	XM_021468090.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio family with sequence similarity 65, member B (fam65b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468090.1

ORF Insert Sequence:

ATGGAACTGT CACGAAAGCT CCAATGGATC CGCGAGCGGC CCTGGAGGGC CCGGGCCCGT 
CACAGGAAGT TCAACAGAGT GTCCTCACGC TTCCCAGACA TCATGGCTGC TGGCACTCAC 
TCTCCTGGAG GACCCAATGG CATCATCAGG AGCCAGTCGT TCGCTGGCTT CAGCACCCTG 
CAGGAGAGAC GCTCACGGTG CAACTCCTTC ATGGGGAACT CGGCAGTGCA GAAGAAGCCC 
ATCTCCAAGC CCAGGAAGCC CCATCTGTCC GGGCATAAAT CCAGCAGCAG CAGCAGCAGC 
TCACGAGAGC CACAGCCTAA ACGTGTAGAG GAGGTTTACA GCGCACTCAA GCAAGGCCTG 
GATGAATATC TGGAGGTGCA CCAAACCGAG CTGGATAAAC TCACTTCCCT CATGAAGGAC 
ATGAAGAGGA ACTCCAGACT GGGTGTGCTG TATGATCTTG ACAAGCAAAT CAAGACCATA 
GAGCGATACA TGCGACGACT GGAGTTTCAC ATCAGCAAGG TCGATGAGCT GTACGAGGGC 
TTTTGCATTC AGAGGAGGTT GCGTGATGGC GCCAGTAAGA TGAAACAGGC CTTCACTGCT 
TCGCCCTCCA CCAAAGGCAC ACGTGAGAGC CTGGCGGAGG TCAATCGCAG ATACAAAGAA 
TATACAGAGA ACATGAGCAC TTTTGAGGGC GAGCTGGAGA ACCTGTTGGG GGAGTTTCAT 
ATCAAAATGA AAGGATTGGC TGGTTTTGCC AGACTTTGTC CTGGTGATCA GTATGAGATT 
TTCATGAGAT ACGGCCGACA GCGGTGGAAA CTGAAGGGGA AGATCGAAGG AAACTCTAAG 
CAGAGTTGGG ATGGAGAGGA GATGATCTTC ATGCCCCTTA TTTCAGACCT TATTAACATC 
AAGGTGACCG AGTTAAAGGG TTTGGCCACT CATGTTTTGG TGGGCAGTGT GATCTGTGAA 
ACCAAGGACC TGTTCACCGC TATGCCTCAG GTGGTTGCTG TAGACGTCAA TGACCTGGGA 
ACCATTAAAC TTAACCTGGA GGTCGCCTGG TTTCCGTTTG ACGTGGAGGA TCTGACTCTG 
TCCTCTGGGA ACGTGAGTAA AGCCACTGCC CTTCAGAGAC GGGTTTCAGT CTATAGCCAA 
GGCACACCCG AGACGCCCAC TTTCCAGGAC ACATCTTTCT TTTCCACTTT ACCAGATGAC 
GTCTTTGAGA ATGGAGGCTG CGGTGTGGCT GAATGCAAGC GTCTGTCCTT CACCTTCTCA 
GACACGTCCA CCTCAAGCCC CGTCCCTGTC CAGTCAAACC CTGAGATTAC AGTTACCCCT 
CCCGAGACGG ACCCATCATC TGAACCACCT CCAACCTCAG AGGAGCAGCT GGTTGAGGAG 
GAGTGTGTAG AAGAAGACTG CGGCAGTATC AGCGTCAGTG TCAGCGTCAG TGATCCGGAC 
CTCGAGTGGG ACCAGGTTGA AACCCAGGGG CCTGAAACAG GCTCTGTGGC CCTCTCGTTG 
TGCTCTGAGA GTCAGCTTTC TGCAGTAGGA CCAGAGGACG TGGTGTTTCT GGAGCCCAAT 
GTGTCTGATG AAGCCCTGGA GCTGAAGCCG GTGGAGCTGG ATGGGGAGGA AGGCAGTCTG 
ACCAGGCAGC TGGTGAGGAG GCTGACCTCG TCTGATATGC TGCCTGAGAC CAGTGCTCTG 
AGCTGGGCTG GAGAGAACAG CAGGACTTTT ATGGAGAGCA GTCTGGAGGA GGTCATTCAG 
TCGCTGCTCC TGCGGCTAGA GAGTTTAGGG CAGCGCTGCG GGGAACTGCA GGACCTGGAG 
CAGGAAGTCA TGAGACTTGA AGACCTGCTC AAGTGTCGAG TGCATGCCCA TAGGAGCCGC 
TCATCAAGCC TCAGTTTGAC GGTAGAAAGC GCTCTGGAAA GTTTTGACTT CCTCAACACC 
TCTGACTTCG ATGACGATGA CACAGGAGAT GACGCTGGCA CTCTCTCACG TGCTGTCTTC 
TTTGACATGG AGTCAGAGAG AATGGGGTCA GGTCAACATC CAGAAGCGCG AGGCCATCTG 
AGTGAAGCCC TCACAGAGGA CACAGGCGTG GGGAACAGTG TGGCCGGCAG CCCTTTGCCC 
CTCACGACAG GAAACGAGAA CCTAGACGTA GCCGTCGTCA TTCATCTGCA ATACTGCAAT 
CATCTCATTC AGTTGTTGAC TTCAGGAGGA AGCCAGTTAC AGCATAAGAC ATACCTGCAC 
AAACTAGCAA CTCAAACTCT GCTGTTAGAG GAGCTCAGTG AGAGGAACTT TGACAGACCG 
AGCAGCTGCA CTTCAGTGCC TGATGTGCTG CCCGGGCTGG TGGAGCGGCC GGCGCTGATG 
TCTCTATGGT CCGACTGCAG CGGTGGGCTT TTCCACACCA CACTAGACCG AGTATTAAAG 
CACATGCATC TCAGCTTTGC CTTAGGACTG CAACAGGTTC TGCCTCACTC TCCTGACTCA 
GTGATCAAGA TGGTGGTGAA TGAGATGATG GACAGGAGTG AACTGGCCTC CTCCTCAACT 
TCTCCAGCAC AGGACGTCAT CACTGTGTTT CAGTTCCACC GCTACATTGT TGAACACTGT 
ATCACAGACA TGGAGCAACA CCTGCTGGAT TTAGCCAAAG AGGTCATGCT TGAAGAAGCC 
CTGAGCTGTG GAGATCCAGA ACGCTCTCTG AAGGAACTAA AGAAAGTGTC CATCACGTGT 
CTTCAGCCCC GTTCTCAAAC TCTATTGGCT GTGGCTGGTC TGCTGACCAC TGATGACCCT 
GAAGTGATGA AGGCCACCAC AGACTTCCTC TCCTCTGCTG CCTCACATCA GCCCTTCAGG 
TCAAAGGCTG TTGATTGTTA CACACAAGCC TTATCAGAAA GTGAGTTACA CACACAGAGA 
GCTGCATGTG CTGCTCTGAG CTGCCTTCAG GCTGTGGAGA GCATTGATGC TGTCATTTTA 
CTGTGTGACT CTGTGGATGA AGAGCTCCGG CAGATTGCTA TTGAAACCCT CCTCACTTTC 
GGTGAGGATG GGCGGCTGGC ATACGAGCAA CTGGACACCG TCCCGAGGGA GATGGTGCGG 
CTGGGCACTC GGCGTGGAAA TGCGGTGACC ACTTCATTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FAM65B ( NM_001045026.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001038491.1 Danio rerio RHO family interacting cell polarization regulator 2 (ripor2), mRNA.

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CloneID	ODa04084
Clone ID Related Accession (Same CDS sequence)	NM_001045026.1
Accession Version	NM_001045026.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-09-29
Organism	Danio rerio(zebrafish)
Product	rho family-interacting cell polarization regulator 2
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX957279.13. On Sep 1, 2006 this sequence version replaced XM_687163.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCCTTTC CTGACATCAC AGAGGATGAC CTAAATGAAC TGATGCGTGA GGAGGCAGAG 
GATGTTTTTG ATGATGACGG AGTGTCCTCA CGCTTCCCAG ACATCATGGC TGCTGGCACT 
CACTCTCCTG GAGGACCCAA TGGCATCATC AGGAGCCAGT CGTTCGCTGG CTTCAGCACC 
CTGCAGGAGA GACGCTCACG GTGCAACTCC TTCATGGGGA ACTCGGCAGT GCAGAAGAAG 
CCCATCTCCA AGCCCAGGAA GCCCCATCTG TCCGGGCATA AATCCAGCAG CAGCAGCAGC 
AGCTCACGAG AGCCACAGCC TAAACGTGTA GAGGAGGTTT ACAGCGCACT CAAGCAAGGC 
CTGGATGAAT ATCTGGAGGT GCACCAAACC GAGCTGGATA AACTCACTTC CCTCATGAAG 
GACATGAAGA GGAACTCCAG ACTGGGTGTG CTGTATGATC TTGACAAGCA AATCAAGACC 
ATAGAGCGAT ACATGCGACG ACTGGAGTTT CACATCAGCA AGGTCGATGA GCTGTACGAG 
GGCTTTTGCA TTCAGAGGAG GTTGCGTGAT GGCGCCAGTA AGATGAAACA GGCCTTCACT 
GCTTCGCCCT CCACCAAAGG CACACGTGAG AGCCTGGCGG AGGTCAATCG CAGATACAAA 
GAATATACAG AGAACATGAG CACTTTTGAG GGCGAGCTGG AGAACCTGTT GGGGGAGTTT 
CATATCAAAA TGAAAGGATT GGCTGGTTTT GCCAGACTTT GTCCTGGTGA TCAGTATGAG 
ATTTTCATGA GATACGGCCG ACAGCGGTGG AAACTGAAGG GGAAGATCGA AGGAAACTCT 
AAGCAGAGTT GGGATGGAGA GGAGATGATC TTCATGCCCC TTATTTCAGA CCTTATTAAC 
ATCAAGGTGA CCGAGTTAAA GGGTTTGGCC ACTCATGTTT TGGTGGGCAG TGTGATCTGT 
GAAACCAAGG ACCTGTTCAC CGCTATGCCT CAGGTGGTTG CTGTAGACGT CAATGACCTG 
GGAACCATTA AACTTAACCT GGAGGTCGCC TGGTTTCCGT TTGACGTGGA GGATCTGACT 
CTGTCCTCTG GGAACGTGAG TAAAGCCACT GCCCTTCAGA GACGGGTTTC AGTCTATAGC 
CAAGGCACAC CCGAGACGCC CACTTTCCAG GACACATCTT TCTTTAAGTG GCAGCCCAGG 
CGATGGGAAG GCCAGCGTCT CTCGTTTTTG CACACACTCA GAGAGACTCT GATAGAGAAA 
CTCAGACGCA GCCGCTCTTT CGGCGATCTG GCCGCACTGA GACCTCGCTC AAGGTCCAGT 
CTAGAAGTCT ACTCCACTTT ACCAGATGAC GTCTTTGAGA ATGGAGGCTG CGGTGTGGCT 
GAATGCAAGC GTCTGTCCTT CACCTTCTCA GACACGTCCA CCTCAAGCCC CGTCCCTGTC 
CAGTCAAACC CTGAGATTAC AGTTACCCCT CCCGAGACGG ACCCATCATC TGAACCACCT 
CCAACCTCAG AGGAGCAGCT GGTTGAGGAG GAGTGTGTAG AAGAAGACTG CGGCAGTATC 
AGCGTCAGTG TCAGCGTCAG TGATCCGGAC CTCGAGTGGG ACCAGGTTGA AACCCAGGGG 
CCTGAAACAG GCTCTGTGGC CCTCTCGTTG TGCTCTGAGA GTCAGCTTTC TGCAGTAGGA 
CCAGAGGACG TGGTGTTTCT GGAGCCCAAT GTGTCTGATG AAGCCCTGGA GCTGAAGCCG 
GTGGAGCTGG ATGGGGAGGA AGGCAGTCTG ACCAGGCAGC TGGTGAGGAG GCTGACCTCG 
TCTGATATGC TGCCTGAGAC CAGTGCTCTG AGCTGGGCTG GAGAGAACAG CAGGACTTTT 
ATGGAGAGCA GTCTGGAGGA GGTCATTCAG TCGCTGCTCC TGCGGCTAGA GAGTTTAGGG 
CAGCGCTGCG GGGAACTGCA GGACCTGGAG CAGGAAGTCA TGAGACTTGA AGACCTGCTC 
AAGTGTCGAG TGCATGCCCA TAGGAGCCGC TCATCAAGCC TCAGTTTGAC GGTAGAAAGC 
GCTCTGGAAA GTTTTGACTT CCTCAACACC TCTGACTTCG ATGACGATGA CACAGGAGAT 
GACGCTGGCA CTCTCTCACG TGCTGTCTTC TTTGACATGG AGTCAGAGAG AATGGGGTCA 
GGTCAACATC CAGAAGCGCG AGGCCATCTG AGTGAAGCCC TCACAGAGGA CACAGGCGTG 
GGGAACAGTG TGGCCGGCAG CCCTTTGCCC CTCACGACAG GAAACGAGAA CCTAGACGTA 
GCCGTCGTCA TTCATCTGCA ATACTGCAAT CATCTCATTC AGTTGTTGAC TTCAGGAGGA 
AGCCAGTTAC AGCATAAGAC ATACCTGCAC AAACTAGCAA CTCAAACTCT GCTGTTAGAG 
GAGCTCAGTG AGAGGAACTT TGACAGACCG AGCAGCTGCA CTTCAGTGCC TGATGTGCTG 
CCCGGGCTGG TGGAGCGGCC GGCGCTGATG TCTCTATGGT CCGACTGCAG CGGTGGGCTT 
TTCCACACCA CACTAGACCG AGTATTAAAG CACATGCATC TCAGCTTTGC CTTAGGACTG 
CAACAGGTTC TGCCTCACTC TCCTGACTCA GTGATCAAGA TGGTGGTGAA TGAGATGATG 
GACAGGAGTG AACTGGCCTC CTCCTCAACT TCTCCAGCAC AGGACGTCAT CACTGTGTTT 
CAGTTCCACC GCTACATTGT TGAACACTGT ATCACAGACA TGGAGCAACA CCTGCTGGAT 
TTAGCCAAAG AGGTCATGCT TGAAGAAGCC CTGAGCTGTG GAGATCCAGA ACGCTCTCTG 
AAGGAACTAA AGAAAGTGTC CATCACGTGT CTTCAGCCCC GTTCTCAAAC TCTATTGGCT 
GTGGCTGGTC TGCTGACCAC TGATGACCCT GAAGTGATGA AGGCCACCAC AGACTTCCTC 
TCCTCTGCTG CCTCACATCA GCCCTTCAGG TCAAAGGCTG TTGATTGTTA CACACAAGCC 
TTATCAGAAA GTGAGTTACA CACACAGAGA GCTGCATGTG CTGCTCTGAG CTGCCTTCAG 
GCTGTGGAGA GCATTGATGC TGTCATTTTA CTGTGTGACT CTGTGGATGA AGAGCTCCGG 
CAGATTGCTA TTGAAACCCT CCTCACTTTC GGTGAGGATG GGCGGCTGGC ATACGAGCAA 
CTGGACACCG TCCCGAGGGA GATGGTGCGG CTGGGCACTC GGCGTGGAAA TGCGGTGACC 
ACTTCATTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001038491.1
CDS	68..3319
Translation	MAFPDITEDDLNELMREEAEDVFDDDGVSSRFPDIMAAGTHSPGGPNGIIRSQSFAGFSTLQERRSRCNSFMGNSAVQKKPISKPRKPHLSGHKSSSSSSSSREPQPKRVEEVYSALKQGLDEYLEVHQTELDKLTSLMKDMKRNSRLGVLYDLDKQIKTIERYMRRLEFHISKVDELYEGFCIQRRLRDGASKMKQAFTASPSTKGTRESLAEVNRRYKEYTENMSTFEGELENLLGEFHIKMKGLAGFARLCPGDQYEIFMRYGRQRWKLKGKIEGNSKQSWDGEEMIFMPLISDLINIKVTELKGLATHVLVGSVICETKDLFTAMPQVVAVDVNDLGTIKLNLEVAWFPFDVEDLTLSSGNVSKATALQRRVSVYSQGTPETPTFQDTSFFKWQPRRWEGQRLSFLHTLRETLIEKLRRSRSFGDLAALRPRSRSSLEVYSTLPDDVFENGGCGVAECKRLSFTFSDTSTSSPVPVQSNPEITVTPPETDPSSEPPPTSEEQLVEEECVEEDCGSISVSVSVSDPDLEWDQVETQGPETGSVALSLCSESQLSAVGPEDVVFLEPNVSDEALELKPVELDGEEGSLTRQLVRRLTSSDMLPETSALSWAGENSRTFMESSLEEVIQSLLLRLESLGQRCGELQDLEQEVMRLEDLLKCRVHAHRSRSSSLSLTVESALESFDFLNTSDFDDDDTGDDAGTLSRAVFFDMESERMGSGQHPEARGHLSEALTEDTGVGNSVAGSPLPLTTGNENLDVAVVIHLQYCNHLIQLLTSGGSQLQHKTYLHKLATQTLLLEELSERNFDRPSSCTSVPDVLPGLVERPALMSLWSDCSGGLFHTTLDRVLKHMHLSFALGLQQVLPHSPDSVIKMVVNEMMDRSELASSSTSPAQDVITVFQFHRYIVEHCITDMEQHLLDLAKEVMLEEALSCGDPERSLKELKKVSITCLQPRSQTLLAVAGLLTTDDPEVMKATTDFLSSAASHQPFRSKAVDCYTQALSESELHTQRAACAALSCLQAVESIDAVILLCDSVDEELRQIAIETLLTFGEDGRLAYEQLDTVPREMVRLGTRRGNAVTTSF

Target ORF information:

RefSeq Version	NM_001045026.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio RHO family interacting cell polarization regulator 2 (ripor2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001045026.1

ORF Insert Sequence:

ATGGCCTTTC CTGACATCAC AGAGGATGAC CTAAATGAAC TGATGCGTGA GGAGGCAGAG 
GATGTTTTTG ATGATGACGG AGTGTCCTCA CGCTTCCCAG ACATCATGGC TGCTGGCACT 
CACTCTCCTG GAGGACCCAA TGGCATCATC AGGAGCCAGT CGTTCGCTGG CTTCAGCACC 
CTGCAGGAGA GACGCTCACG GTGCAACTCC TTCATGGGGA ACTCGGCAGT GCAGAAGAAG 
CCCATCTCCA AGCCCAGGAA GCCCCATCTG TCCGGGCATA AATCCAGCAG CAGCAGCAGC 
AGCTCACGAG AGCCACAGCC TAAACGTGTA GAGGAGGTTT ACAGCGCACT CAAGCAAGGC 
CTGGATGAAT ATCTGGAGGT GCACCAAACC GAGCTGGATA AACTCACTTC CCTCATGAAG 
GACATGAAGA GGAACTCCAG ACTGGGTGTG CTGTATGATC TTGACAAGCA AATCAAGACC 
ATAGAGCGAT ACATGCGACG ACTGGAGTTT CACATCAGCA AGGTCGATGA GCTGTACGAG 
GGCTTTTGCA TTCAGAGGAG GTTGCGTGAT GGCGCCAGTA AGATGAAACA GGCCTTCACT 
GCTTCGCCCT CCACCAAAGG CACACGTGAG AGCCTGGCGG AGGTCAATCG CAGATACAAA 
GAATATACAG AGAACATGAG CACTTTTGAG GGCGAGCTGG AGAACCTGTT GGGGGAGTTT 
CATATCAAAA TGAAAGGATT GGCTGGTTTT GCCAGACTTT GTCCTGGTGA TCAGTATGAG 
ATTTTCATGA GATACGGCCG ACAGCGGTGG AAACTGAAGG GGAAGATCGA AGGAAACTCT 
AAGCAGAGTT GGGATGGAGA GGAGATGATC TTCATGCCCC TTATTTCAGA CCTTATTAAC 
ATCAAGGTGA CCGAGTTAAA GGGTTTGGCC ACTCATGTTT TGGTGGGCAG TGTGATCTGT 
GAAACCAAGG ACCTGTTCAC CGCTATGCCT CAGGTGGTTG CTGTAGACGT CAATGACCTG 
GGAACCATTA AACTTAACCT GGAGGTCGCC TGGTTTCCGT TTGACGTGGA GGATCTGACT 
CTGTCCTCTG GGAACGTGAG TAAAGCCACT GCCCTTCAGA GACGGGTTTC AGTCTATAGC 
CAAGGCACAC CCGAGACGCC CACTTTCCAG GACACATCTT TCTTTAAGTG GCAGCCCAGG 
CGATGGGAAG GCCAGCGTCT CTCGTTTTTG CACACACTCA GAGAGACTCT GATAGAGAAA 
CTCAGACGCA GCCGCTCTTT CGGCGATCTG GCCGCACTGA GACCTCGCTC AAGGTCCAGT 
CTAGAAGTCT ACTCCACTTT ACCAGATGAC GTCTTTGAGA ATGGAGGCTG CGGTGTGGCT 
GAATGCAAGC GTCTGTCCTT CACCTTCTCA GACACGTCCA CCTCAAGCCC CGTCCCTGTC 
CAGTCAAACC CTGAGATTAC AGTTACCCCT CCCGAGACGG ACCCATCATC TGAACCACCT 
CCAACCTCAG AGGAGCAGCT GGTTGAGGAG GAGTGTGTAG AAGAAGACTG CGGCAGTATC 
AGCGTCAGTG TCAGCGTCAG TGATCCGGAC CTCGAGTGGG ACCAGGTTGA AACCCAGGGG 
CCTGAAACAG GCTCTGTGGC CCTCTCGTTG TGCTCTGAGA GTCAGCTTTC TGCAGTAGGA 
CCAGAGGACG TGGTGTTTCT GGAGCCCAAT GTGTCTGATG AAGCCCTGGA GCTGAAGCCG 
GTGGAGCTGG ATGGGGAGGA AGGCAGTCTG ACCAGGCAGC TGGTGAGGAG GCTGACCTCG 
TCTGATATGC TGCCTGAGAC CAGTGCTCTG AGCTGGGCTG GAGAGAACAG CAGGACTTTT 
ATGGAGAGCA GTCTGGAGGA GGTCATTCAG TCGCTGCTCC TGCGGCTAGA GAGTTTAGGG 
CAGCGCTGCG GGGAACTGCA GGACCTGGAG CAGGAAGTCA TGAGACTTGA AGACCTGCTC 
AAGTGTCGAG TGCATGCCCA TAGGAGCCGC TCATCAAGCC TCAGTTTGAC GGTAGAAAGC 
GCTCTGGAAA GTTTTGACTT CCTCAACACC TCTGACTTCG ATGACGATGA CACAGGAGAT 
GACGCTGGCA CTCTCTCACG TGCTGTCTTC TTTGACATGG AGTCAGAGAG AATGGGGTCA 
GGTCAACATC CAGAAGCGCG AGGCCATCTG AGTGAAGCCC TCACAGAGGA CACAGGCGTG 
GGGAACAGTG TGGCCGGCAG CCCTTTGCCC CTCACGACAG GAAACGAGAA CCTAGACGTA 
GCCGTCGTCA TTCATCTGCA ATACTGCAAT CATCTCATTC AGTTGTTGAC TTCAGGAGGA 
AGCCAGTTAC AGCATAAGAC ATACCTGCAC AAACTAGCAA CTCAAACTCT GCTGTTAGAG 
GAGCTCAGTG AGAGGAACTT TGACAGACCG AGCAGCTGCA CTTCAGTGCC TGATGTGCTG 
CCCGGGCTGG TGGAGCGGCC GGCGCTGATG TCTCTATGGT CCGACTGCAG CGGTGGGCTT 
TTCCACACCA CACTAGACCG AGTATTAAAG CACATGCATC TCAGCTTTGC CTTAGGACTG 
CAACAGGTTC TGCCTCACTC TCCTGACTCA GTGATCAAGA TGGTGGTGAA TGAGATGATG 
GACAGGAGTG AACTGGCCTC CTCCTCAACT TCTCCAGCAC AGGACGTCAT CACTGTGTTT 
CAGTTCCACC GCTACATTGT TGAACACTGT ATCACAGACA TGGAGCAACA CCTGCTGGAT 
TTAGCCAAAG AGGTCATGCT TGAAGAAGCC CTGAGCTGTG GAGATCCAGA ACGCTCTCTG 
AAGGAACTAA AGAAAGTGTC CATCACGTGT CTTCAGCCCC GTTCTCAAAC TCTATTGGCT 
GTGGCTGGTC TGCTGACCAC TGATGACCCT GAAGTGATGA AGGCCACCAC AGACTTCCTC 
TCCTCTGCTG CCTCACATCA GCCCTTCAGG TCAAAGGCTG TTGATTGTTA CACACAAGCC 
TTATCAGAAA GTGAGTTACA CACACAGAGA GCTGCATGTG CTGCTCTGAG CTGCCTTCAG 
GCTGTGGAGA GCATTGATGC TGTCATTTTA CTGTGTGACT CTGTGGATGA AGAGCTCCGG 
CAGATTGCTA TTGAAACCCT CCTCACTTTC GGTGAGGATG GGCGGCTGGC ATACGAGCAA 
CTGGACACCG TCCCGAGGGA GATGGTGCGG CTGGGCACTC GGCGTGGAAA TGCGGTGACC 
ACTTCATTTT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FAM65B ( XM_005159634.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005159691.1 Danio rerio family with sequence similarity 65, member B (fam65b), transcript variant X1, mRNA.

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CloneID	ODa24292
Clone ID Related Accession (Same CDS sequence)	XM_005159634.3 , XM_005159634.4
Accession Version	XM_005159634.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3105bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein FAM65B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005159634.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTTTC CTGACATCAC AGAGGATGAC CTAAATGAAC TGATGCGTGA GGAGGCAGAG 
GATGTTTTTG ATGATGACGG AGTGTCCTCA CGCTTCCCAG ACATCATGGC TGCTGGCACT 
CACTCTCCTG GAGGACCCAA TGGCATCATC AGGAGCCAGT CGTTCGCTGG CTTCAGCACC 
CTGCAGGAGA GACGCTCACG GTGCAACTCC TTCATGGGGA ACTCGGCAGT GCAGAAGAAG 
CCCATCTCCA AGCCCAGGAA GCCCCATCTG TCCGGGCATA AATCCAGCAG CAGCAGCAGC 
AGCTCACGAG AGCCACAGCC TAAACGTGTA GAGGAGGTTT ACAGCGCACT CAAGCAAGGC 
CTGGATGAAT ATCTGGAGGT GCACCAAACC GAGCTGGATA AACTCACTTC CCTCATGAAG 
GACATGAAGA GGAACTCCAG ACTGGGTGTG CTGTATGATC TTGACAAGCA AATCAAGACC 
ATAGAGCGAT ACATGCGACG ACTGGAGTTT CACATCAGCA AGGTCGATGA GCTGTACGAG 
GGCTTTTGCA TTCAGAGGAG GTTGCGTGAT GGCGCCAGTA AGATGAAACA GGCCTTCACT 
GCTTCGCCCT CCACCAAAGG CACACGTGAG AGCCTGGCGG AGGTCAATCG CAGATACAAA 
GAATATACAG AGAACATGAG CACTTTTGAG GGCGAGCTGG AGAACCTGTT GGGGGAGTTT 
CATATCAAAA TGAAAGGATT GGCTGGTTTT GCCAGACTTT GTCCTGGTGA TCAGTATGAG 
ATTTTCATGA GATACGGCCG ACAGCGGTGG AAACTGAAGG GGAAGATCGA AGGAAACTCT 
AAGCAGAGTT GGGATGGAGA GGAGATGATC TTCATGCCCC TTATTTCAGA CCTTATTAAC 
ATCAAGGTGA CCGAGTTAAA GGGTTTGGCC ACTCATGTTT TGGTGGGCAG TGTGATCTGT 
GAAACCAAGG ACCTGTTCAC CGCTATGCCT CAGGTGGTTG CTGTAGACGT CAATGACCTG 
GGAACCATTA AACTTAACCT GGAGGTCGCC TGGTTTCCGT TTGACGTGGA GGATCTGACT 
CTGTCCTCTG GGAACGTGAG TAAAGCCACT GCCCTTCAGA GACGGGTTTC AGTCTATAGC 
CAAGGCACAC CCGAGACGCC CACTTTCCAG GACACATCTT TCTTTTCCAC TTTACCAGAT 
GACGTCTTTG AGAATGGAGG CTGCGGTGTG GCTGAATGCA AGCGTCTGTC CTTCACCTTC 
TCAGACACGT CCACCTCAAG CCCCGTCCCT GTCCAGTCAA ACCCTGAGAT TACAGTTACC 
CCTCCCGAGA CGGACCCATC ATCTGAACCA CCTCCAACCT CAGAGGAGCA GCTGGTTGAG 
GAGGAGTGTG TAGAAGAAGA CTGCGGCAGT ATCAGCGTCA GTGTCAGCGT CAGTGATCCG 
GACCTCGAGT GGGACCAGGT TGAAACCCAG GGGCCTGAAA CAGGCTCTGT GGCCCTCTCG 
TTGTGCTCTG AGAGTCAGCT TTCTGCAGTA GGACCAGAGG ACGTGGTGTT TCTGGAGCCC 
AATGTGTCTG ATGAAGCCCT GGAGCTGAAG CCGGTGGAGC TGGATGGGGA GGAAGGCAGT 
CTGACCAGGC AGCTGGTGAG GAGGCTGACC TCGTCTGATA TGCTGCCTGA GACCAGTGCT 
CTGAGCTGGG CTGGAGAGAA CAGCAGGACT TTTATGGAGA GCAGTCTGGA GGAGGTCATT 
CAGTCGCTGC TCCTGCGGCT AGAGAGTTTA GGGCAGCGCT GCGGGGAACT GCAGGACCTG 
GAGCAGGAAG TCATGAGACT TGAAGACCTG CTCAAGTGTC GAGTGCATGC CCATAGGAGC 
CGCTCATCAA GCCTCAGTTT GACGGTAGAA AGCGCTCTGG AAAGTTTTGA CTTCCTCAAC 
ACCTCTGACT TCGATGACGA TGACACAGGA GATGACGCTG GCACTCTCTC ACGTGCTGTC 
TTCTTTGACA TGGAGTCAGA GAGAATGGGG TCAGGTCAAC ATCCAGAAGC GCGAGGCCAT 
CTGAGTGAAG CCCTCACAGA GGACACAGGC GTGGGGAACA GTGTGGCCGG CAGCCCTTTG 
CCCCTCACGA CAGGAAACGA GAACCTAGAC GTAGCCGTCG TCATTCATCT GCAATACTGC 
AATCATCTCA TTCAGTTGTT GACTTCAGGA GGAAGCCAGT TACAGCATAA GACATACCTG 
CACAAACTAG CAACTCAAAC TCTGCTGTTA GAGGAGCTCA GTGAGAGGAA CTTTGACAGA 
CCGAGCAGCT GCACTTCAGT GCCTGATGTG CTGCCCGGGC TGGTGGAGCG GCCGGCGCTG 
ATGTCTCTAT GGTCCGACTG CAGCGGTGGG CTTTTCCACA CCACACTAGA CCGAGTATTA 
AAGCACATGC ATCTCAGCTT TGCCTTAGGA CTGCAACAGG TTCTGCCTCA CTCTCCTGAC 
TCAGTGATCA AGATGGTGGT GAATGAGATG ATGGACAGGA GTGAACTGGC CTCCTCCTCA 
ACTTCTCCAG CACAGGACGT CATCACTGTG TTTCAGTTCC ACCGCTACAT TGTTGAACAC 
TGTATCACAG ACATGGAGCA ACACCTGCTG GATTTAGCCA AAGAGGTCAT GCTTGAAGAA 
GCCCTGAGCT GTGGAGATCC AGAACGCTCT CTGAAGGAAC TAAAGAAAGT GTCCATCACG 
TGTCTTCAGC CCCGTTCTCA AACTCTATTG GCTGTGGCTG GTCTGCTGAC CACTGATGAC 
CCTGAAGTGA TGAAGGCCAC CACAGACTTC CTCTCCTCTG CTGCCTCACA TCAGCCCTTC 
AGGTCAAAGG CTGTTGATTG TTACACACAA GCCTTATCAG AAAGTGAGTT ACACACACAG 
AGAGCTGCAT GTGCTGCTCT GAGCTGCCTT CAGGCTGTGG AGAGCATTGA TGCTGTCATT 
TTACTGTGTG ACTCTGTGGA TGAAGAGCTC CGGCAGATTG CTATTGAAAC CCTCCTCACT 
TTCGGTGAGG ATGGGCGGCT GGCATACGAG CAACTGGACA CCGTCCCGAG GGAGATGGTG 
CGGCTGGGCA CTCGGCGTGG AAATGCGGTG ACCACTTCAT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159691.1
CDS	119..3223
Translation	MAFPDITEDDLNELMREEAEDVFDDDGVSSRFPDIMAAGTHSPGGPNGIIRSQSFAGFSTLQERRSRCNSFMGNSAVQKKPISKPRKPHLSGHKSSSSSSSSREPQPKRVEEVYSALKQGLDEYLEVHQTELDKLTSLMKDMKRNSRLGVLYDLDKQIKTIERYMRRLEFHISKVDELYEGFCIQRRLRDGASKMKQAFTASPSTKGTRESLAEVNRRYKEYTENMSTFEGELENLLGEFHIKMKGLAGFARLCPGDQYEIFMRYGRQRWKLKGKIEGNSKQSWDGEEMIFMPLISDLINIKVTELKGLATHVLVGSVICETKDLFTAMPQVVAVDVNDLGTIKLNLEVAWFPFDVEDLTLSSGNVSKATALQRRVSVYSQGTPETPTFQDTSFFSTLPDDVFENGGCGVAECKRLSFTFSDTSTSSPVPVQSNPEITVTPPETDPSSEPPPTSEEQLVEEECVEEDCGSISVSVSVSDPDLEWDQVETQGPETGSVALSLCSESQLSAVGPEDVVFLEPNVSDEALELKPVELDGEEGSLTRQLVRRLTSSDMLPETSALSWAGENSRTFMESSLEEVIQSLLLRLESLGQRCGELQDLEQEVMRLEDLLKCRVHAHRSRSSSLSLTVESALESFDFLNTSDFDDDDTGDDAGTLSRAVFFDMESERMGSGQHPEARGHLSEALTEDTGVGNSVAGSPLPLTTGNENLDVAVVIHLQYCNHLIQLLTSGGSQLQHKTYLHKLATQTLLLEELSERNFDRPSSCTSVPDVLPGLVERPALMSLWSDCSGGLFHTTLDRVLKHMHLSFALGLQQVLPHSPDSVIKMVVNEMMDRSELASSSTSPAQDVITVFQFHRYIVEHCITDMEQHLLDLAKEVMLEEALSCGDPERSLKELKKVSITCLQPRSQTLLAVAGLLTTDDPEVMKATTDFLSSAASHQPFRSKAVDCYTQALSESELHTQRAACAALSCLQAVESIDAVILLCDSVDEELRQIAIETLLTFGEDGRLAYEQLDTVPREMVRLGTRRGNAVTTSF

Target ORF information:

RefSeq Version	XM_005159634.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio family with sequence similarity 65, member B (fam65b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159634.3

ORF Insert Sequence:

ATGGCCTTTC CTGACATCAC AGAGGATGAC CTAAATGAAC TGATGCGTGA GGAGGCAGAG 
GATGTTTTTG ATGATGACGG AGTGTCCTCA CGCTTCCCAG ACATCATGGC TGCTGGCACT 
CACTCTCCTG GAGGACCCAA TGGCATCATC AGGAGCCAGT CGTTCGCTGG CTTCAGCACC 
CTGCAGGAGA GACGCTCACG GTGCAACTCC TTCATGGGGA ACTCGGCAGT GCAGAAGAAG 
CCCATCTCCA AGCCCAGGAA GCCCCATCTG TCCGGGCATA AATCCAGCAG CAGCAGCAGC 
AGCTCACGAG AGCCACAGCC TAAACGTGTA GAGGAGGTTT ACAGCGCACT CAAGCAAGGC 
CTGGATGAAT ATCTGGAGGT GCACCAAACC GAGCTGGATA AACTCACTTC CCTCATGAAG 
GACATGAAGA GGAACTCCAG ACTGGGTGTG CTGTATGATC TTGACAAGCA AATCAAGACC 
ATAGAGCGAT ACATGCGACG ACTGGAGTTT CACATCAGCA AGGTCGATGA GCTGTACGAG 
GGCTTTTGCA TTCAGAGGAG GTTGCGTGAT GGCGCCAGTA AGATGAAACA GGCCTTCACT 
GCTTCGCCCT CCACCAAAGG CACACGTGAG AGCCTGGCGG AGGTCAATCG CAGATACAAA 
GAATATACAG AGAACATGAG CACTTTTGAG GGCGAGCTGG AGAACCTGTT GGGGGAGTTT 
CATATCAAAA TGAAAGGATT GGCTGGTTTT GCCAGACTTT GTCCTGGTGA TCAGTATGAG 
ATTTTCATGA GATACGGCCG ACAGCGGTGG AAACTGAAGG GGAAGATCGA AGGAAACTCT 
AAGCAGAGTT GGGATGGAGA GGAGATGATC TTCATGCCCC TTATTTCAGA CCTTATTAAC 
ATCAAGGTGA CCGAGTTAAA GGGTTTGGCC ACTCATGTTT TGGTGGGCAG TGTGATCTGT 
GAAACCAAGG ACCTGTTCAC CGCTATGCCT CAGGTGGTTG CTGTAGACGT CAATGACCTG 
GGAACCATTA AACTTAACCT GGAGGTCGCC TGGTTTCCGT TTGACGTGGA GGATCTGACT 
CTGTCCTCTG GGAACGTGAG TAAAGCCACT GCCCTTCAGA GACGGGTTTC AGTCTATAGC 
CAAGGCACAC CCGAGACGCC CACTTTCCAG GACACATCTT TCTTTTCCAC TTTACCAGAT 
GACGTCTTTG AGAATGGAGG CTGCGGTGTG GCTGAATGCA AGCGTCTGTC CTTCACCTTC 
TCAGACACGT CCACCTCAAG CCCCGTCCCT GTCCAGTCAA ACCCTGAGAT TACAGTTACC 
CCTCCCGAGA CGGACCCATC ATCTGAACCA CCTCCAACCT CAGAGGAGCA GCTGGTTGAG 
GAGGAGTGTG TAGAAGAAGA CTGCGGCAGT ATCAGCGTCA GTGTCAGCGT CAGTGATCCG 
GACCTCGAGT GGGACCAGGT TGAAACCCAG GGGCCTGAAA CAGGCTCTGT GGCCCTCTCG 
TTGTGCTCTG AGAGTCAGCT TTCTGCAGTA GGACCAGAGG ACGTGGTGTT TCTGGAGCCC 
AATGTGTCTG ATGAAGCCCT GGAGCTGAAG CCGGTGGAGC TGGATGGGGA GGAAGGCAGT 
CTGACCAGGC AGCTGGTGAG GAGGCTGACC TCGTCTGATA TGCTGCCTGA GACCAGTGCT 
CTGAGCTGGG CTGGAGAGAA CAGCAGGACT TTTATGGAGA GCAGTCTGGA GGAGGTCATT 
CAGTCGCTGC TCCTGCGGCT AGAGAGTTTA GGGCAGCGCT GCGGGGAACT GCAGGACCTG 
GAGCAGGAAG TCATGAGACT TGAAGACCTG CTCAAGTGTC GAGTGCATGC CCATAGGAGC 
CGCTCATCAA GCCTCAGTTT GACGGTAGAA AGCGCTCTGG AAAGTTTTGA CTTCCTCAAC 
ACCTCTGACT TCGATGACGA TGACACAGGA GATGACGCTG GCACTCTCTC ACGTGCTGTC 
TTCTTTGACA TGGAGTCAGA GAGAATGGGG TCAGGTCAAC ATCCAGAAGC GCGAGGCCAT 
CTGAGTGAAG CCCTCACAGA GGACACAGGC GTGGGGAACA GTGTGGCCGG CAGCCCTTTG 
CCCCTCACGA CAGGAAACGA GAACCTAGAC GTAGCCGTCG TCATTCATCT GCAATACTGC 
AATCATCTCA TTCAGTTGTT GACTTCAGGA GGAAGCCAGT TACAGCATAA GACATACCTG 
CACAAACTAG CAACTCAAAC TCTGCTGTTA GAGGAGCTCA GTGAGAGGAA CTTTGACAGA 
CCGAGCAGCT GCACTTCAGT GCCTGATGTG CTGCCCGGGC TGGTGGAGCG GCCGGCGCTG 
ATGTCTCTAT GGTCCGACTG CAGCGGTGGG CTTTTCCACA CCACACTAGA CCGAGTATTA 
AAGCACATGC ATCTCAGCTT TGCCTTAGGA CTGCAACAGG TTCTGCCTCA CTCTCCTGAC 
TCAGTGATCA AGATGGTGGT GAATGAGATG ATGGACAGGA GTGAACTGGC CTCCTCCTCA 
ACTTCTCCAG CACAGGACGT CATCACTGTG TTTCAGTTCC ACCGCTACAT TGTTGAACAC 
TGTATCACAG ACATGGAGCA ACACCTGCTG GATTTAGCCA AAGAGGTCAT GCTTGAAGAA 
GCCCTGAGCT GTGGAGATCC AGAACGCTCT CTGAAGGAAC TAAAGAAAGT GTCCATCACG 
TGTCTTCAGC CCCGTTCTCA AACTCTATTG GCTGTGGCTG GTCTGCTGAC CACTGATGAC 
CCTGAAGTGA TGAAGGCCAC CACAGACTTC CTCTCCTCTG CTGCCTCACA TCAGCCCTTC 
AGGTCAAAGG CTGTTGATTG TTACACACAA GCCTTATCAG AAAGTGAGTT ACACACACAG 
AGAGCTGCAT GTGCTGCTCT GAGCTGCCTT CAGGCTGTGG AGAGCATTGA TGCTGTCATT 
TTACTGTGTG ACTCTGTGGA TGAAGAGCTC CGGCAGATTG CTATTGAAAC CCTCCTCACT 
TTCGGTGAGG ATGGGCGGCT GGCATACGAG CAACTGGACA CCGTCCCGAG GGAGATGGTG 
CGGCTGGGCA CTCGGCGTGG AAATGCGGTG ACCACTTCAT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FAM65B ( XM_005159634.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005159691.1 Danio rerio family with sequence similarity 65, member B (fam65b), transcript variant X1, mRNA.

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CloneID	ODa24292
Clone ID Related Accession (Same CDS sequence)	XM_005159634.3 , XM_005159634.4
Accession Version	XM_005159634.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3105bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein FAM65B isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005159634.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCCTTTC CTGACATCAC AGAGGATGAC CTAAATGAAC TGATGCGTGA GGAGGCAGAG 
GATGTTTTTG ATGATGACGG AGTGTCCTCA CGCTTCCCAG ACATCATGGC TGCTGGCACT 
CACTCTCCTG GAGGACCCAA TGGCATCATC AGGAGCCAGT CGTTCGCTGG CTTCAGCACC 
CTGCAGGAGA GACGCTCACG GTGCAACTCC TTCATGGGGA ACTCGGCAGT GCAGAAGAAG 
CCCATCTCCA AGCCCAGGAA GCCCCATCTG TCCGGGCATA AATCCAGCAG CAGCAGCAGC 
AGCTCACGAG AGCCACAGCC TAAACGTGTA GAGGAGGTTT ACAGCGCACT CAAGCAAGGC 
CTGGATGAAT ATCTGGAGGT GCACCAAACC GAGCTGGATA AACTCACTTC CCTCATGAAG 
GACATGAAGA GGAACTCCAG ACTGGGTGTG CTGTATGATC TTGACAAGCA AATCAAGACC 
ATAGAGCGAT ACATGCGACG ACTGGAGTTT CACATCAGCA AGGTCGATGA GCTGTACGAG 
GGCTTTTGCA TTCAGAGGAG GTTGCGTGAT GGCGCCAGTA AGATGAAACA GGCCTTCACT 
GCTTCGCCCT CCACCAAAGG CACACGTGAG AGCCTGGCGG AGGTCAATCG CAGATACAAA 
GAATATACAG AGAACATGAG CACTTTTGAG GGCGAGCTGG AGAACCTGTT GGGGGAGTTT 
CATATCAAAA TGAAAGGATT GGCTGGTTTT GCCAGACTTT GTCCTGGTGA TCAGTATGAG 
ATTTTCATGA GATACGGCCG ACAGCGGTGG AAACTGAAGG GGAAGATCGA AGGAAACTCT 
AAGCAGAGTT GGGATGGAGA GGAGATGATC TTCATGCCCC TTATTTCAGA CCTTATTAAC 
ATCAAGGTGA CCGAGTTAAA GGGTTTGGCC ACTCATGTTT TGGTGGGCAG TGTGATCTGT 
GAAACCAAGG ACCTGTTCAC CGCTATGCCT CAGGTGGTTG CTGTAGACGT CAATGACCTG 
GGAACCATTA AACTTAACCT GGAGGTCGCC TGGTTTCCGT TTGACGTGGA GGATCTGACT 
CTGTCCTCTG GGAACGTGAG TAAAGCCACT GCCCTTCAGA GACGGGTTTC AGTCTATAGC 
CAAGGCACAC CCGAGACGCC CACTTTCCAG GACACATCTT TCTTTTCCAC TTTACCAGAT 
GACGTCTTTG AGAATGGAGG CTGCGGTGTG GCTGAATGCA AGCGTCTGTC CTTCACCTTC 
TCAGACACGT CCACCTCAAG CCCCGTCCCT GTCCAGTCAA ACCCTGAGAT TACAGTTACC 
CCTCCCGAGA CGGACCCATC ATCTGAACCA CCTCCAACCT CAGAGGAGCA GCTGGTTGAG 
GAGGAGTGTG TAGAAGAAGA CTGCGGCAGT ATCAGCGTCA GTGTCAGCGT CAGTGATCCG 
GACCTCGAGT GGGACCAGGT TGAAACCCAG GGGCCTGAAA CAGGCTCTGT GGCCCTCTCG 
TTGTGCTCTG AGAGTCAGCT TTCTGCAGTA GGACCAGAGG ACGTGGTGTT TCTGGAGCCC 
AATGTGTCTG ATGAAGCCCT GGAGCTGAAG CCGGTGGAGC TGGATGGGGA GGAAGGCAGT 
CTGACCAGGC AGCTGGTGAG GAGGCTGACC TCGTCTGATA TGCTGCCTGA GACCAGTGCT 
CTGAGCTGGG CTGGAGAGAA CAGCAGGACT TTTATGGAGA GCAGTCTGGA GGAGGTCATT 
CAGTCGCTGC TCCTGCGGCT AGAGAGTTTA GGGCAGCGCT GCGGGGAACT GCAGGACCTG 
GAGCAGGAAG TCATGAGACT TGAAGACCTG CTCAAGTGTC GAGTGCATGC CCATAGGAGC 
CGCTCATCAA GCCTCAGTTT GACGGTAGAA AGCGCTCTGG AAAGTTTTGA CTTCCTCAAC 
ACCTCTGACT TCGATGACGA TGACACAGGA GATGACGCTG GCACTCTCTC ACGTGCTGTC 
TTCTTTGACA TGGAGTCAGA GAGAATGGGG TCAGGTCAAC ATCCAGAAGC GCGAGGCCAT 
CTGAGTGAAG CCCTCACAGA GGACACAGGC GTGGGGAACA GTGTGGCCGG CAGCCCTTTG 
CCCCTCACGA CAGGAAACGA GAACCTAGAC GTAGCCGTCG TCATTCATCT GCAATACTGC 
AATCATCTCA TTCAGTTGTT GACTTCAGGA GGAAGCCAGT TACAGCATAA GACATACCTG 
CACAAACTAG CAACTCAAAC TCTGCTGTTA GAGGAGCTCA GTGAGAGGAA CTTTGACAGA 
CCGAGCAGCT GCACTTCAGT GCCTGATGTG CTGCCCGGGC TGGTGGAGCG GCCGGCGCTG 
ATGTCTCTAT GGTCCGACTG CAGCGGTGGG CTTTTCCACA CCACACTAGA CCGAGTATTA 
AAGCACATGC ATCTCAGCTT TGCCTTAGGA CTGCAACAGG TTCTGCCTCA CTCTCCTGAC 
TCAGTGATCA AGATGGTGGT GAATGAGATG ATGGACAGGA GTGAACTGGC CTCCTCCTCA 
ACTTCTCCAG CACAGGACGT CATCACTGTG TTTCAGTTCC ACCGCTACAT TGTTGAACAC 
TGTATCACAG ACATGGAGCA ACACCTGCTG GATTTAGCCA AAGAGGTCAT GCTTGAAGAA 
GCCCTGAGCT GTGGAGATCC AGAACGCTCT CTGAAGGAAC TAAAGAAAGT GTCCATCACG 
TGTCTTCAGC CCCGTTCTCA AACTCTATTG GCTGTGGCTG GTCTGCTGAC CACTGATGAC 
CCTGAAGTGA TGAAGGCCAC CACAGACTTC CTCTCCTCTG CTGCCTCACA TCAGCCCTTC 
AGGTCAAAGG CTGTTGATTG TTACACACAA GCCTTATCAG AAAGTGAGTT ACACACACAG 
AGAGCTGCAT GTGCTGCTCT GAGCTGCCTT CAGGCTGTGG AGAGCATTGA TGCTGTCATT 
TTACTGTGTG ACTCTGTGGA TGAAGAGCTC CGGCAGATTG CTATTGAAAC CCTCCTCACT 
TTCGGTGAGG ATGGGCGGCT GGCATACGAG CAACTGGACA CCGTCCCGAG GGAGATGGTG 
CGGCTGGGCA CTCGGCGTGG AAATGCGGTG ACCACTTCAT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159691.1
CDS	115..3219
Translation	MAFPDITEDDLNELMREEAEDVFDDDGVSSRFPDIMAAGTHSPGGPNGIIRSQSFAGFSTLQERRSRCNSFMGNSAVQKKPISKPRKPHLSGHKSSSSSSSSREPQPKRVEEVYSALKQGLDEYLEVHQTELDKLTSLMKDMKRNSRLGVLYDLDKQIKTIERYMRRLEFHISKVDELYEGFCIQRRLRDGASKMKQAFTASPSTKGTRESLAEVNRRYKEYTENMSTFEGELENLLGEFHIKMKGLAGFARLCPGDQYEIFMRYGRQRWKLKGKIEGNSKQSWDGEEMIFMPLISDLINIKVTELKGLATHVLVGSVICETKDLFTAMPQVVAVDVNDLGTIKLNLEVAWFPFDVEDLTLSSGNVSKATALQRRVSVYSQGTPETPTFQDTSFFSTLPDDVFENGGCGVAECKRLSFTFSDTSTSSPVPVQSNPEITVTPPETDPSSEPPPTSEEQLVEEECVEEDCGSISVSVSVSDPDLEWDQVETQGPETGSVALSLCSESQLSAVGPEDVVFLEPNVSDEALELKPVELDGEEGSLTRQLVRRLTSSDMLPETSALSWAGENSRTFMESSLEEVIQSLLLRLESLGQRCGELQDLEQEVMRLEDLLKCRVHAHRSRSSSLSLTVESALESFDFLNTSDFDDDDTGDDAGTLSRAVFFDMESERMGSGQHPEARGHLSEALTEDTGVGNSVAGSPLPLTTGNENLDVAVVIHLQYCNHLIQLLTSGGSQLQHKTYLHKLATQTLLLEELSERNFDRPSSCTSVPDVLPGLVERPALMSLWSDCSGGLFHTTLDRVLKHMHLSFALGLQQVLPHSPDSVIKMVVNEMMDRSELASSSTSPAQDVITVFQFHRYIVEHCITDMEQHLLDLAKEVMLEEALSCGDPERSLKELKKVSITCLQPRSQTLLAVAGLLTTDDPEVMKATTDFLSSAASHQPFRSKAVDCYTQALSESELHTQRAACAALSCLQAVESIDAVILLCDSVDEELRQIAIETLLTFGEDGRLAYEQLDTVPREMVRLGTRRGNAVTTSF

Target ORF information:

RefSeq Version	XM_005159634.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio family with sequence similarity 65, member B (fam65b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005159634.4

ORF Insert Sequence:

ATGGCCTTTC CTGACATCAC AGAGGATGAC CTAAATGAAC TGATGCGTGA GGAGGCAGAG 
GATGTTTTTG ATGATGACGG AGTGTCCTCA CGCTTCCCAG ACATCATGGC TGCTGGCACT 
CACTCTCCTG GAGGACCCAA TGGCATCATC AGGAGCCAGT CGTTCGCTGG CTTCAGCACC 
CTGCAGGAGA GACGCTCACG GTGCAACTCC TTCATGGGGA ACTCGGCAGT GCAGAAGAAG 
CCCATCTCCA AGCCCAGGAA GCCCCATCTG TCCGGGCATA AATCCAGCAG CAGCAGCAGC 
AGCTCACGAG AGCCACAGCC TAAACGTGTA GAGGAGGTTT ACAGCGCACT CAAGCAAGGC 
CTGGATGAAT ATCTGGAGGT GCACCAAACC GAGCTGGATA AACTCACTTC CCTCATGAAG 
GACATGAAGA GGAACTCCAG ACTGGGTGTG CTGTATGATC TTGACAAGCA AATCAAGACC 
ATAGAGCGAT ACATGCGACG ACTGGAGTTT CACATCAGCA AGGTCGATGA GCTGTACGAG 
GGCTTTTGCA TTCAGAGGAG GTTGCGTGAT GGCGCCAGTA AGATGAAACA GGCCTTCACT 
GCTTCGCCCT CCACCAAAGG CACACGTGAG AGCCTGGCGG AGGTCAATCG CAGATACAAA 
GAATATACAG AGAACATGAG CACTTTTGAG GGCGAGCTGG AGAACCTGTT GGGGGAGTTT 
CATATCAAAA TGAAAGGATT GGCTGGTTTT GCCAGACTTT GTCCTGGTGA TCAGTATGAG 
ATTTTCATGA GATACGGCCG ACAGCGGTGG AAACTGAAGG GGAAGATCGA AGGAAACTCT 
AAGCAGAGTT GGGATGGAGA GGAGATGATC TTCATGCCCC TTATTTCAGA CCTTATTAAC 
ATCAAGGTGA CCGAGTTAAA GGGTTTGGCC ACTCATGTTT TGGTGGGCAG TGTGATCTGT 
GAAACCAAGG ACCTGTTCAC CGCTATGCCT CAGGTGGTTG CTGTAGACGT CAATGACCTG 
GGAACCATTA AACTTAACCT GGAGGTCGCC TGGTTTCCGT TTGACGTGGA GGATCTGACT 
CTGTCCTCTG GGAACGTGAG TAAAGCCACT GCCCTTCAGA GACGGGTTTC AGTCTATAGC 
CAAGGCACAC CCGAGACGCC CACTTTCCAG GACACATCTT TCTTTTCCAC TTTACCAGAT 
GACGTCTTTG AGAATGGAGG CTGCGGTGTG GCTGAATGCA AGCGTCTGTC CTTCACCTTC 
TCAGACACGT CCACCTCAAG CCCCGTCCCT GTCCAGTCAA ACCCTGAGAT TACAGTTACC 
CCTCCCGAGA CGGACCCATC ATCTGAACCA CCTCCAACCT CAGAGGAGCA GCTGGTTGAG 
GAGGAGTGTG TAGAAGAAGA CTGCGGCAGT ATCAGCGTCA GTGTCAGCGT CAGTGATCCG 
GACCTCGAGT GGGACCAGGT TGAAACCCAG GGGCCTGAAA CAGGCTCTGT GGCCCTCTCG 
TTGTGCTCTG AGAGTCAGCT TTCTGCAGTA GGACCAGAGG ACGTGGTGTT TCTGGAGCCC 
AATGTGTCTG ATGAAGCCCT GGAGCTGAAG CCGGTGGAGC TGGATGGGGA GGAAGGCAGT 
CTGACCAGGC AGCTGGTGAG GAGGCTGACC TCGTCTGATA TGCTGCCTGA GACCAGTGCT 
CTGAGCTGGG CTGGAGAGAA CAGCAGGACT TTTATGGAGA GCAGTCTGGA GGAGGTCATT 
CAGTCGCTGC TCCTGCGGCT AGAGAGTTTA GGGCAGCGCT GCGGGGAACT GCAGGACCTG 
GAGCAGGAAG TCATGAGACT TGAAGACCTG CTCAAGTGTC GAGTGCATGC CCATAGGAGC 
CGCTCATCAA GCCTCAGTTT GACGGTAGAA AGCGCTCTGG AAAGTTTTGA CTTCCTCAAC 
ACCTCTGACT TCGATGACGA TGACACAGGA GATGACGCTG GCACTCTCTC ACGTGCTGTC 
TTCTTTGACA TGGAGTCAGA GAGAATGGGG TCAGGTCAAC ATCCAGAAGC GCGAGGCCAT 
CTGAGTGAAG CCCTCACAGA GGACACAGGC GTGGGGAACA GTGTGGCCGG CAGCCCTTTG 
CCCCTCACGA CAGGAAACGA GAACCTAGAC GTAGCCGTCG TCATTCATCT GCAATACTGC 
AATCATCTCA TTCAGTTGTT GACTTCAGGA GGAAGCCAGT TACAGCATAA GACATACCTG 
CACAAACTAG CAACTCAAAC TCTGCTGTTA GAGGAGCTCA GTGAGAGGAA CTTTGACAGA 
CCGAGCAGCT GCACTTCAGT GCCTGATGTG CTGCCCGGGC TGGTGGAGCG GCCGGCGCTG 
ATGTCTCTAT GGTCCGACTG CAGCGGTGGG CTTTTCCACA CCACACTAGA CCGAGTATTA 
AAGCACATGC ATCTCAGCTT TGCCTTAGGA CTGCAACAGG TTCTGCCTCA CTCTCCTGAC 
TCAGTGATCA AGATGGTGGT GAATGAGATG ATGGACAGGA GTGAACTGGC CTCCTCCTCA 
ACTTCTCCAG CACAGGACGT CATCACTGTG TTTCAGTTCC ACCGCTACAT TGTTGAACAC 
TGTATCACAG ACATGGAGCA ACACCTGCTG GATTTAGCCA AAGAGGTCAT GCTTGAAGAA 
GCCCTGAGCT GTGGAGATCC AGAACGCTCT CTGAAGGAAC TAAAGAAAGT GTCCATCACG 
TGTCTTCAGC CCCGTTCTCA AACTCTATTG GCTGTGGCTG GTCTGCTGAC CACTGATGAC 
CCTGAAGTGA TGAAGGCCAC CACAGACTTC CTCTCCTCTG CTGCCTCACA TCAGCCCTTC 
AGGTCAAAGG CTGTTGATTG TTACACACAA GCCTTATCAG AAAGTGAGTT ACACACACAG 
AGAGCTGCAT GTGCTGCTCT GAGCTGCCTT CAGGCTGTGG AGAGCATTGA TGCTGTCATT 
TTACTGTGTG ACTCTGTGGA TGAAGAGCTC CGGCAGATTG CTATTGAAAC CCTCCTCACT 
TTCGGTGAGG ATGGGCGGCT GGCATACGAG CAACTGGACA CCGTCCCGAG GGAGATGGTG 
CGGCTGGGCA CTCGGCGTGG AAATGCGGTG ACCACTTCAT TTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FAM65B ( XM_009294297.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009292572.1 Danio rerio family with sequence similarity 65, member B (fam65b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa44190
Clone ID Related Accession (Same CDS sequence)	XM_009294297.3 , XM_009294297.2
Accession Version	XM_009294297.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3069bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein FAM65B isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294297.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTTTG ATCCGTTGCT GTCGTGCCGG ATGGTTTCGC TCGGAGTGTC CTCACGCTTC 
CCAGACATCA TGGCTGCTGG CACTCACTCT CCTGGAGGAC CCAATGGCAT CATCAGGAGC 
CAGTCGTTCG CTGGCTTCAG CACCCTGCAG GAGAGACGCT CACGGTGCAA CTCCTTCATG 
GGGAACTCGG CAGTGCAGAA GAAGCCCATC TCCAAGCCCA GGAAGCCCCA TCTGTCCGGG 
CATAAATCCA GCAGCAGCAG CAGCAGCTCA CGAGAGCCAC AGCCTAAACG TGTAGAGGAG 
GTTTACAGCG CACTCAAGCA AGGCCTGGAT GAATATCTGG AGGTGCACCA AACCGAGCTG 
GATAAACTCA CTTCCCTCAT GAAGGACATG AAGAGGAACT CCAGACTGGG TGTGCTGTAT 
GATCTTGACA AGCAAATCAA GACCATAGAG CGATACATGC GACGACTGGA GTTTCACATC 
AGCAAGGTCG ATGAGCTGTA CGAGGGCTTT TGCATTCAGA GGAGGTTGCG TGATGGCGCC 
AGTAAGATGA AACAGGCCTT CACTGCTTCG CCCTCCACCA AAGGCACACG TGAGAGCCTG 
GCGGAGGTCA ATCGCAGATA CAAAGAATAT ACAGAGAACA TGAGCACTTT TGAGGGCGAG 
CTGGAGAACC TGTTGGGGGA GTTTCATATC AAAATGAAAG GATTGGCTGG TTTTGCCAGA 
CTTTGTCCTG GTGATCAGTA TGAGATTTTC ATGAGATACG GCCGACAGCG GTGGAAACTG 
AAGGGGAAGA TCGAAGGAAA CTCTAAGCAG AGTTGGGATG GAGAGGAGAT GATCTTCATG 
CCCCTTATTT CAGACCTTAT TAACATCAAG GTGACCGAGT TAAAGGGTTT GGCCACTCAT 
GTTTTGGTGG GCAGTGTGAT CTGTGAAACC AAGGACCTGT TCACCGCTAT GCCTCAGGTG 
GTTGCTGTAG ACGTCAATGA CCTGGGAACC ATTAAACTTA ACCTGGAGGT CGCCTGGTTT 
CCGTTTGACG TGGAGGATCT GACTCTGTCC TCTGGGAACG TGAGTAAAGC CACTGCCCTT 
CAGAGACGGG TTTCAGTCTA TAGCCAAGGC ACACCCGAGA CGCCCACTTT CCAGGACACA 
TCTTTCTTTT CCACTTTACC AGATGACGTC TTTGAGAATG GAGGCTGCGG TGTGGCTGAA 
TGCAAGCGTC TGTCCTTCAC CTTCTCAGAC ACGTCCACCT CAAGCCCCGT CCCTGTCCAG 
TCAAACCCTG AGATTACAGT TACCCCTCCC GAGACGGACC CATCATCTGA ACCACCTCCA 
ACCTCAGAGG AGCAGCTGGT TGAGGAGGAG TGTGTAGAAG AAGACTGCGG CAGTATCAGC 
GTCAGTGTCA GCGTCAGTGA TCCGGACCTC GAGTGGGACC AGGTTGAAAC CCAGGGGCCT 
GAAACAGGCT CTGTGGCCCT CTCGTTGTGC TCTGAGAGTC AGCTTTCTGC AGTAGGACCA 
GAGGACGTGG TGTTTCTGGA GCCCAATGTG TCTGATGAAG CCCTGGAGCT GAAGCCGGTG 
GAGCTGGATG GGGAGGAAGG CAGTCTGACC AGGCAGCTGG TGAGGAGGCT GACCTCGTCT 
GATATGCTGC CTGAGACCAG TGCTCTGAGC TGGGCTGGAG AGAACAGCAG GACTTTTATG 
GAGAGCAGTC TGGAGGAGGT CATTCAGTCG CTGCTCCTGC GGCTAGAGAG TTTAGGGCAG 
CGCTGCGGGG AACTGCAGGA CCTGGAGCAG GAAGTCATGA GACTTGAAGA CCTGCTCAAG 
TGTCGAGTGC ATGCCCATAG GAGCCGCTCA TCAAGCCTCA GTTTGACGGT AGAAAGCGCT 
CTGGAAAGTT TTGACTTCCT CAACACCTCT GACTTCGATG ACGATGACAC AGGAGATGAC 
GCTGGCACTC TCTCACGTGC TGTCTTCTTT GACATGGAGT CAGAGAGAAT GGGGTCAGGT 
CAACATCCAG AAGCGCGAGG CCATCTGAGT GAAGCCCTCA CAGAGGACAC AGGCGTGGGG 
AACAGTGTGG CCGGCAGCCC TTTGCCCCTC ACGACAGGAA ACGAGAACCT AGACGTAGCC 
GTCGTCATTC ATCTGCAATA CTGCAATCAT CTCATTCAGT TGTTGACTTC AGGAGGAAGC 
CAGTTACAGC ATAAGACATA CCTGCACAAA CTAGCAACTC AAACTCTGCT GTTAGAGGAG 
CTCAGTGAGA GGAACTTTGA CAGACCGAGC AGCTGCACTT CAGTGCCTGA TGTGCTGCCC 
GGGCTGGTGG AGCGGCCGGC GCTGATGTCT CTATGGTCCG ACTGCAGCGG TGGGCTTTTC 
CACACCACAC TAGACCGAGT ATTAAAGCAC ATGCATCTCA GCTTTGCCTT AGGACTGCAA 
CAGGTTCTGC CTCACTCTCC TGACTCAGTG ATCAAGATGG TGGTGAATGA GATGATGGAC 
AGGAGTGAAC TGGCCTCCTC CTCAACTTCT CCAGCACAGG ACGTCATCAC TGTGTTTCAG 
TTCCACCGCT ACATTGTTGA ACACTGTATC ACAGACATGG AGCAACACCT GCTGGATTTA 
GCCAAAGAGG TCATGCTTGA AGAAGCCCTG AGCTGTGGAG ATCCAGAACG CTCTCTGAAG 
GAACTAAAGA AAGTGTCCAT CACGTGTCTT CAGCCCCGTT CTCAAACTCT ATTGGCTGTG 
GCTGGTCTGC TGACCACTGA TGACCCTGAA GTGATGAAGG CCACCACAGA CTTCCTCTCC 
TCTGCTGCCT CACATCAGCC CTTCAGGTCA AAGGCTGTTG ATTGTTACAC ACAAGCCTTA 
TCAGAAAGTG AGTTACACAC ACAGAGAGCT GCATGTGCTG CTCTGAGCTG CCTTCAGGCT 
GTGGAGAGCA TTGATGCTGT CATTTTACTG TGTGACTCTG TGGATGAAGA GCTCCGGCAG 
ATTGCTATTG AAACCCTCCT CACTTTCGGT GAGGATGGGC GGCTGGCATA CGAGCAACTG 
GACACCGTCC CGAGGGAGAT GGTGCGGCTG GGCACTCGGC GTGGAAATGC GGTGACCACT 
TCATTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292572.1
CDS	18..3086
Translation	MIFDPLLSCRMVSLGVSSRFPDIMAAGTHSPGGPNGIIRSQSFAGFSTLQERRSRCNSFMGNSAVQKKPISKPRKPHLSGHKSSSSSSSSREPQPKRVEEVYSALKQGLDEYLEVHQTELDKLTSLMKDMKRNSRLGVLYDLDKQIKTIERYMRRLEFHISKVDELYEGFCIQRRLRDGASKMKQAFTASPSTKGTRESLAEVNRRYKEYTENMSTFEGELENLLGEFHIKMKGLAGFARLCPGDQYEIFMRYGRQRWKLKGKIEGNSKQSWDGEEMIFMPLISDLINIKVTELKGLATHVLVGSVICETKDLFTAMPQVVAVDVNDLGTIKLNLEVAWFPFDVEDLTLSSGNVSKATALQRRVSVYSQGTPETPTFQDTSFFSTLPDDVFENGGCGVAECKRLSFTFSDTSTSSPVPVQSNPEITVTPPETDPSSEPPPTSEEQLVEEECVEEDCGSISVSVSVSDPDLEWDQVETQGPETGSVALSLCSESQLSAVGPEDVVFLEPNVSDEALELKPVELDGEEGSLTRQLVRRLTSSDMLPETSALSWAGENSRTFMESSLEEVIQSLLLRLESLGQRCGELQDLEQEVMRLEDLLKCRVHAHRSRSSSLSLTVESALESFDFLNTSDFDDDDTGDDAGTLSRAVFFDMESERMGSGQHPEARGHLSEALTEDTGVGNSVAGSPLPLTTGNENLDVAVVIHLQYCNHLIQLLTSGGSQLQHKTYLHKLATQTLLLEELSERNFDRPSSCTSVPDVLPGLVERPALMSLWSDCSGGLFHTTLDRVLKHMHLSFALGLQQVLPHSPDSVIKMVVNEMMDRSELASSSTSPAQDVITVFQFHRYIVEHCITDMEQHLLDLAKEVMLEEALSCGDPERSLKELKKVSITCLQPRSQTLLAVAGLLTTDDPEVMKATTDFLSSAASHQPFRSKAVDCYTQALSESELHTQRAACAALSCLQAVESIDAVILLCDSVDEELRQIAIETLLTFGEDGRLAYEQLDTVPREMVRLGTRRGNAVTTSF

Target ORF information:

RefSeq Version	XM_009294297.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio family with sequence similarity 65, member B (fam65b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294297.3

ORF Insert Sequence:

ATGATTTTTG ATCCGTTGCT GTCGTGCCGG ATGGTTTCGC TCGGAGTGTC CTCACGCTTC 
CCAGACATCA TGGCTGCTGG CACTCACTCT CCTGGAGGAC CCAATGGCAT CATCAGGAGC 
CAGTCGTTCG CTGGCTTCAG CACCCTGCAG GAGAGACGCT CACGGTGCAA CTCCTTCATG 
GGGAACTCGG CAGTGCAGAA GAAGCCCATC TCCAAGCCCA GGAAGCCCCA TCTGTCCGGG 
CATAAATCCA GCAGCAGCAG CAGCAGCTCA CGAGAGCCAC AGCCTAAACG TGTAGAGGAG 
GTTTACAGCG CACTCAAGCA AGGCCTGGAT GAATATCTGG AGGTGCACCA AACCGAGCTG 
GATAAACTCA CTTCCCTCAT GAAGGACATG AAGAGGAACT CCAGACTGGG TGTGCTGTAT 
GATCTTGACA AGCAAATCAA GACCATAGAG CGATACATGC GACGACTGGA GTTTCACATC 
AGCAAGGTCG ATGAGCTGTA CGAGGGCTTT TGCATTCAGA GGAGGTTGCG TGATGGCGCC 
AGTAAGATGA AACAGGCCTT CACTGCTTCG CCCTCCACCA AAGGCACACG TGAGAGCCTG 
GCGGAGGTCA ATCGCAGATA CAAAGAATAT ACAGAGAACA TGAGCACTTT TGAGGGCGAG 
CTGGAGAACC TGTTGGGGGA GTTTCATATC AAAATGAAAG GATTGGCTGG TTTTGCCAGA 
CTTTGTCCTG GTGATCAGTA TGAGATTTTC ATGAGATACG GCCGACAGCG GTGGAAACTG 
AAGGGGAAGA TCGAAGGAAA CTCTAAGCAG AGTTGGGATG GAGAGGAGAT GATCTTCATG 
CCCCTTATTT CAGACCTTAT TAACATCAAG GTGACCGAGT TAAAGGGTTT GGCCACTCAT 
GTTTTGGTGG GCAGTGTGAT CTGTGAAACC AAGGACCTGT TCACCGCTAT GCCTCAGGTG 
GTTGCTGTAG ACGTCAATGA CCTGGGAACC ATTAAACTTA ACCTGGAGGT CGCCTGGTTT 
CCGTTTGACG TGGAGGATCT GACTCTGTCC TCTGGGAACG TGAGTAAAGC CACTGCCCTT 
CAGAGACGGG TTTCAGTCTA TAGCCAAGGC ACACCCGAGA CGCCCACTTT CCAGGACACA 
TCTTTCTTTT CCACTTTACC AGATGACGTC TTTGAGAATG GAGGCTGCGG TGTGGCTGAA 
TGCAAGCGTC TGTCCTTCAC CTTCTCAGAC ACGTCCACCT CAAGCCCCGT CCCTGTCCAG 
TCAAACCCTG AGATTACAGT TACCCCTCCC GAGACGGACC CATCATCTGA ACCACCTCCA 
ACCTCAGAGG AGCAGCTGGT TGAGGAGGAG TGTGTAGAAG AAGACTGCGG CAGTATCAGC 
GTCAGTGTCA GCGTCAGTGA TCCGGACCTC GAGTGGGACC AGGTTGAAAC CCAGGGGCCT 
GAAACAGGCT CTGTGGCCCT CTCGTTGTGC TCTGAGAGTC AGCTTTCTGC AGTAGGACCA 
GAGGACGTGG TGTTTCTGGA GCCCAATGTG TCTGATGAAG CCCTGGAGCT GAAGCCGGTG 
GAGCTGGATG GGGAGGAAGG CAGTCTGACC AGGCAGCTGG TGAGGAGGCT GACCTCGTCT 
GATATGCTGC CTGAGACCAG TGCTCTGAGC TGGGCTGGAG AGAACAGCAG GACTTTTATG 
GAGAGCAGTC TGGAGGAGGT CATTCAGTCG CTGCTCCTGC GGCTAGAGAG TTTAGGGCAG 
CGCTGCGGGG AACTGCAGGA CCTGGAGCAG GAAGTCATGA GACTTGAAGA CCTGCTCAAG 
TGTCGAGTGC ATGCCCATAG GAGCCGCTCA TCAAGCCTCA GTTTGACGGT AGAAAGCGCT 
CTGGAAAGTT TTGACTTCCT CAACACCTCT GACTTCGATG ACGATGACAC AGGAGATGAC 
GCTGGCACTC TCTCACGTGC TGTCTTCTTT GACATGGAGT CAGAGAGAAT GGGGTCAGGT 
CAACATCCAG AAGCGCGAGG CCATCTGAGT GAAGCCCTCA CAGAGGACAC AGGCGTGGGG 
AACAGTGTGG CCGGCAGCCC TTTGCCCCTC ACGACAGGAA ACGAGAACCT AGACGTAGCC 
GTCGTCATTC ATCTGCAATA CTGCAATCAT CTCATTCAGT TGTTGACTTC AGGAGGAAGC 
CAGTTACAGC ATAAGACATA CCTGCACAAA CTAGCAACTC AAACTCTGCT GTTAGAGGAG 
CTCAGTGAGA GGAACTTTGA CAGACCGAGC AGCTGCACTT CAGTGCCTGA TGTGCTGCCC 
GGGCTGGTGG AGCGGCCGGC GCTGATGTCT CTATGGTCCG ACTGCAGCGG TGGGCTTTTC 
CACACCACAC TAGACCGAGT ATTAAAGCAC ATGCATCTCA GCTTTGCCTT AGGACTGCAA 
CAGGTTCTGC CTCACTCTCC TGACTCAGTG ATCAAGATGG TGGTGAATGA GATGATGGAC 
AGGAGTGAAC TGGCCTCCTC CTCAACTTCT CCAGCACAGG ACGTCATCAC TGTGTTTCAG 
TTCCACCGCT ACATTGTTGA ACACTGTATC ACAGACATGG AGCAACACCT GCTGGATTTA 
GCCAAAGAGG TCATGCTTGA AGAAGCCCTG AGCTGTGGAG ATCCAGAACG CTCTCTGAAG 
GAACTAAAGA AAGTGTCCAT CACGTGTCTT CAGCCCCGTT CTCAAACTCT ATTGGCTGTG 
GCTGGTCTGC TGACCACTGA TGACCCTGAA GTGATGAAGG CCACCACAGA CTTCCTCTCC 
TCTGCTGCCT CACATCAGCC CTTCAGGTCA AAGGCTGTTG ATTGTTACAC ACAAGCCTTA 
TCAGAAAGTG AGTTACACAC ACAGAGAGCT GCATGTGCTG CTCTGAGCTG CCTTCAGGCT 
GTGGAGAGCA TTGATGCTGT CATTTTACTG TGTGACTCTG TGGATGAAGA GCTCCGGCAG 
ATTGCTATTG AAACCCTCCT CACTTTCGGT GAGGATGGGC GGCTGGCATA CGAGCAACTG 
GACACCGTCC CGAGGGAGAT GGTGCGGCTG GGCACTCGGC GTGGAAATGC GGTGACCACT 
TCATTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

FAM65B ( XM_009294297.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009292572.1 Danio rerio family with sequence similarity 65, member B (fam65b), transcript variant X2, mRNA.

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CloneID	ODa44190
Clone ID Related Accession (Same CDS sequence)	XM_009294297.3 , XM_009294297.2
Accession Version	XM_009294297.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3069bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein FAM65B isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007130.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294297.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTTTG ATCCGTTGCT GTCGTGCCGG ATGGTTTCGC TCGGAGTGTC CTCACGCTTC 
CCAGACATCA TGGCTGCTGG CACTCACTCT CCTGGAGGAC CCAATGGCAT CATCAGGAGC 
CAGTCGTTCG CTGGCTTCAG CACCCTGCAG GAGAGACGCT CACGGTGCAA CTCCTTCATG 
GGGAACTCGG CAGTGCAGAA GAAGCCCATC TCCAAGCCCA GGAAGCCCCA TCTGTCCGGG 
CATAAATCCA GCAGCAGCAG CAGCAGCTCA CGAGAGCCAC AGCCTAAACG TGTAGAGGAG 
GTTTACAGCG CACTCAAGCA AGGCCTGGAT GAATATCTGG AGGTGCACCA AACCGAGCTG 
GATAAACTCA CTTCCCTCAT GAAGGACATG AAGAGGAACT CCAGACTGGG TGTGCTGTAT 
GATCTTGACA AGCAAATCAA GACCATAGAG CGATACATGC GACGACTGGA GTTTCACATC 
AGCAAGGTCG ATGAGCTGTA CGAGGGCTTT TGCATTCAGA GGAGGTTGCG TGATGGCGCC 
AGTAAGATGA AACAGGCCTT CACTGCTTCG CCCTCCACCA AAGGCACACG TGAGAGCCTG 
GCGGAGGTCA ATCGCAGATA CAAAGAATAT ACAGAGAACA TGAGCACTTT TGAGGGCGAG 
CTGGAGAACC TGTTGGGGGA GTTTCATATC AAAATGAAAG GATTGGCTGG TTTTGCCAGA 
CTTTGTCCTG GTGATCAGTA TGAGATTTTC ATGAGATACG GCCGACAGCG GTGGAAACTG 
AAGGGGAAGA TCGAAGGAAA CTCTAAGCAG AGTTGGGATG GAGAGGAGAT GATCTTCATG 
CCCCTTATTT CAGACCTTAT TAACATCAAG GTGACCGAGT TAAAGGGTTT GGCCACTCAT 
GTTTTGGTGG GCAGTGTGAT CTGTGAAACC AAGGACCTGT TCACCGCTAT GCCTCAGGTG 
GTTGCTGTAG ACGTCAATGA CCTGGGAACC ATTAAACTTA ACCTGGAGGT CGCCTGGTTT 
CCGTTTGACG TGGAGGATCT GACTCTGTCC TCTGGGAACG TGAGTAAAGC CACTGCCCTT 
CAGAGACGGG TTTCAGTCTA TAGCCAAGGC ACACCCGAGA CGCCCACTTT CCAGGACACA 
TCTTTCTTTT CCACTTTACC AGATGACGTC TTTGAGAATG GAGGCTGCGG TGTGGCTGAA 
TGCAAGCGTC TGTCCTTCAC CTTCTCAGAC ACGTCCACCT CAAGCCCCGT CCCTGTCCAG 
TCAAACCCTG AGATTACAGT TACCCCTCCC GAGACGGACC CATCATCTGA ACCACCTCCA 
ACCTCAGAGG AGCAGCTGGT TGAGGAGGAG TGTGTAGAAG AAGACTGCGG CAGTATCAGC 
GTCAGTGTCA GCGTCAGTGA TCCGGACCTC GAGTGGGACC AGGTTGAAAC CCAGGGGCCT 
GAAACAGGCT CTGTGGCCCT CTCGTTGTGC TCTGAGAGTC AGCTTTCTGC AGTAGGACCA 
GAGGACGTGG TGTTTCTGGA GCCCAATGTG TCTGATGAAG CCCTGGAGCT GAAGCCGGTG 
GAGCTGGATG GGGAGGAAGG CAGTCTGACC AGGCAGCTGG TGAGGAGGCT GACCTCGTCT 
GATATGCTGC CTGAGACCAG TGCTCTGAGC TGGGCTGGAG AGAACAGCAG GACTTTTATG 
GAGAGCAGTC TGGAGGAGGT CATTCAGTCG CTGCTCCTGC GGCTAGAGAG TTTAGGGCAG 
CGCTGCGGGG AACTGCAGGA CCTGGAGCAG GAAGTCATGA GACTTGAAGA CCTGCTCAAG 
TGTCGAGTGC ATGCCCATAG GAGCCGCTCA TCAAGCCTCA GTTTGACGGT AGAAAGCGCT 
CTGGAAAGTT TTGACTTCCT CAACACCTCT GACTTCGATG ACGATGACAC AGGAGATGAC 
GCTGGCACTC TCTCACGTGC TGTCTTCTTT GACATGGAGT CAGAGAGAAT GGGGTCAGGT 
CAACATCCAG AAGCGCGAGG CCATCTGAGT GAAGCCCTCA CAGAGGACAC AGGCGTGGGG 
AACAGTGTGG CCGGCAGCCC TTTGCCCCTC ACGACAGGAA ACGAGAACCT AGACGTAGCC 
GTCGTCATTC ATCTGCAATA CTGCAATCAT CTCATTCAGT TGTTGACTTC AGGAGGAAGC 
CAGTTACAGC ATAAGACATA CCTGCACAAA CTAGCAACTC AAACTCTGCT GTTAGAGGAG 
CTCAGTGAGA GGAACTTTGA CAGACCGAGC AGCTGCACTT CAGTGCCTGA TGTGCTGCCC 
GGGCTGGTGG AGCGGCCGGC GCTGATGTCT CTATGGTCCG ACTGCAGCGG TGGGCTTTTC 
CACACCACAC TAGACCGAGT ATTAAAGCAC ATGCATCTCA GCTTTGCCTT AGGACTGCAA 
CAGGTTCTGC CTCACTCTCC TGACTCAGTG ATCAAGATGG TGGTGAATGA GATGATGGAC 
AGGAGTGAAC TGGCCTCCTC CTCAACTTCT CCAGCACAGG ACGTCATCAC TGTGTTTCAG 
TTCCACCGCT ACATTGTTGA ACACTGTATC ACAGACATGG AGCAACACCT GCTGGATTTA 
GCCAAAGAGG TCATGCTTGA AGAAGCCCTG AGCTGTGGAG ATCCAGAACG CTCTCTGAAG 
GAACTAAAGA AAGTGTCCAT CACGTGTCTT CAGCCCCGTT CTCAAACTCT ATTGGCTGTG 
GCTGGTCTGC TGACCACTGA TGACCCTGAA GTGATGAAGG CCACCACAGA CTTCCTCTCC 
TCTGCTGCCT CACATCAGCC CTTCAGGTCA AAGGCTGTTG ATTGTTACAC ACAAGCCTTA 
TCAGAAAGTG AGTTACACAC ACAGAGAGCT GCATGTGCTG CTCTGAGCTG CCTTCAGGCT 
GTGGAGAGCA TTGATGCTGT CATTTTACTG TGTGACTCTG TGGATGAAGA GCTCCGGCAG 
ATTGCTATTG AAACCCTCCT CACTTTCGGT GAGGATGGGC GGCTGGCATA CGAGCAACTG 
GACACCGTCC CGAGGGAGAT GGTGCGGCTG GGCACTCGGC GTGGAAATGC GGTGACCACT 
TCATTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292572.1
CDS	19..3087
Translation	MIFDPLLSCRMVSLGVSSRFPDIMAAGTHSPGGPNGIIRSQSFAGFSTLQERRSRCNSFMGNSAVQKKPISKPRKPHLSGHKSSSSSSSSREPQPKRVEEVYSALKQGLDEYLEVHQTELDKLTSLMKDMKRNSRLGVLYDLDKQIKTIERYMRRLEFHISKVDELYEGFCIQRRLRDGASKMKQAFTASPSTKGTRESLAEVNRRYKEYTENMSTFEGELENLLGEFHIKMKGLAGFARLCPGDQYEIFMRYGRQRWKLKGKIEGNSKQSWDGEEMIFMPLISDLINIKVTELKGLATHVLVGSVICETKDLFTAMPQVVAVDVNDLGTIKLNLEVAWFPFDVEDLTLSSGNVSKATALQRRVSVYSQGTPETPTFQDTSFFSTLPDDVFENGGCGVAECKRLSFTFSDTSTSSPVPVQSNPEITVTPPETDPSSEPPPTSEEQLVEEECVEEDCGSISVSVSVSDPDLEWDQVETQGPETGSVALSLCSESQLSAVGPEDVVFLEPNVSDEALELKPVELDGEEGSLTRQLVRRLTSSDMLPETSALSWAGENSRTFMESSLEEVIQSLLLRLESLGQRCGELQDLEQEVMRLEDLLKCRVHAHRSRSSSLSLTVESALESFDFLNTSDFDDDDTGDDAGTLSRAVFFDMESERMGSGQHPEARGHLSEALTEDTGVGNSVAGSPLPLTTGNENLDVAVVIHLQYCNHLIQLLTSGGSQLQHKTYLHKLATQTLLLEELSERNFDRPSSCTSVPDVLPGLVERPALMSLWSDCSGGLFHTTLDRVLKHMHLSFALGLQQVLPHSPDSVIKMVVNEMMDRSELASSSTSPAQDVITVFQFHRYIVEHCITDMEQHLLDLAKEVMLEEALSCGDPERSLKELKKVSITCLQPRSQTLLAVAGLLTTDDPEVMKATTDFLSSAASHQPFRSKAVDCYTQALSESELHTQRAACAALSCLQAVESIDAVILLCDSVDEELRQIAIETLLTFGEDGRLAYEQLDTVPREMVRLGTRRGNAVTTSF

Target ORF information:

RefSeq Version	XM_009294297.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio family with sequence similarity 65, member B (fam65b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294297.2

ORF Insert Sequence:

ATGATTTTTG ATCCGTTGCT GTCGTGCCGG ATGGTTTCGC TCGGAGTGTC CTCACGCTTC 
CCAGACATCA TGGCTGCTGG CACTCACTCT CCTGGAGGAC CCAATGGCAT CATCAGGAGC 
CAGTCGTTCG CTGGCTTCAG CACCCTGCAG GAGAGACGCT CACGGTGCAA CTCCTTCATG 
GGGAACTCGG CAGTGCAGAA GAAGCCCATC TCCAAGCCCA GGAAGCCCCA TCTGTCCGGG 
CATAAATCCA GCAGCAGCAG CAGCAGCTCA CGAGAGCCAC AGCCTAAACG TGTAGAGGAG 
GTTTACAGCG CACTCAAGCA AGGCCTGGAT GAATATCTGG AGGTGCACCA AACCGAGCTG 
GATAAACTCA CTTCCCTCAT GAAGGACATG AAGAGGAACT CCAGACTGGG TGTGCTGTAT 
GATCTTGACA AGCAAATCAA GACCATAGAG CGATACATGC GACGACTGGA GTTTCACATC 
AGCAAGGTCG ATGAGCTGTA CGAGGGCTTT TGCATTCAGA GGAGGTTGCG TGATGGCGCC 
AGTAAGATGA AACAGGCCTT CACTGCTTCG CCCTCCACCA AAGGCACACG TGAGAGCCTG 
GCGGAGGTCA ATCGCAGATA CAAAGAATAT ACAGAGAACA TGAGCACTTT TGAGGGCGAG 
CTGGAGAACC TGTTGGGGGA GTTTCATATC AAAATGAAAG GATTGGCTGG TTTTGCCAGA 
CTTTGTCCTG GTGATCAGTA TGAGATTTTC ATGAGATACG GCCGACAGCG GTGGAAACTG 
AAGGGGAAGA TCGAAGGAAA CTCTAAGCAG AGTTGGGATG GAGAGGAGAT GATCTTCATG 
CCCCTTATTT CAGACCTTAT TAACATCAAG GTGACCGAGT TAAAGGGTTT GGCCACTCAT 
GTTTTGGTGG GCAGTGTGAT CTGTGAAACC AAGGACCTGT TCACCGCTAT GCCTCAGGTG 
GTTGCTGTAG ACGTCAATGA CCTGGGAACC ATTAAACTTA ACCTGGAGGT CGCCTGGTTT 
CCGTTTGACG TGGAGGATCT GACTCTGTCC TCTGGGAACG TGAGTAAAGC CACTGCCCTT 
CAGAGACGGG TTTCAGTCTA TAGCCAAGGC ACACCCGAGA CGCCCACTTT CCAGGACACA 
TCTTTCTTTT CCACTTTACC AGATGACGTC TTTGAGAATG GAGGCTGCGG TGTGGCTGAA 
TGCAAGCGTC TGTCCTTCAC CTTCTCAGAC ACGTCCACCT CAAGCCCCGT CCCTGTCCAG 
TCAAACCCTG AGATTACAGT TACCCCTCCC GAGACGGACC CATCATCTGA ACCACCTCCA 
ACCTCAGAGG AGCAGCTGGT TGAGGAGGAG TGTGTAGAAG AAGACTGCGG CAGTATCAGC 
GTCAGTGTCA GCGTCAGTGA TCCGGACCTC GAGTGGGACC AGGTTGAAAC CCAGGGGCCT 
GAAACAGGCT CTGTGGCCCT CTCGTTGTGC TCTGAGAGTC AGCTTTCTGC AGTAGGACCA 
GAGGACGTGG TGTTTCTGGA GCCCAATGTG TCTGATGAAG CCCTGGAGCT GAAGCCGGTG 
GAGCTGGATG GGGAGGAAGG CAGTCTGACC AGGCAGCTGG TGAGGAGGCT GACCTCGTCT 
GATATGCTGC CTGAGACCAG TGCTCTGAGC TGGGCTGGAG AGAACAGCAG GACTTTTATG 
GAGAGCAGTC TGGAGGAGGT CATTCAGTCG CTGCTCCTGC GGCTAGAGAG TTTAGGGCAG 
CGCTGCGGGG AACTGCAGGA CCTGGAGCAG GAAGTCATGA GACTTGAAGA CCTGCTCAAG 
TGTCGAGTGC ATGCCCATAG GAGCCGCTCA TCAAGCCTCA GTTTGACGGT AGAAAGCGCT 
CTGGAAAGTT TTGACTTCCT CAACACCTCT GACTTCGATG ACGATGACAC AGGAGATGAC 
GCTGGCACTC TCTCACGTGC TGTCTTCTTT GACATGGAGT CAGAGAGAAT GGGGTCAGGT 
CAACATCCAG AAGCGCGAGG CCATCTGAGT GAAGCCCTCA CAGAGGACAC AGGCGTGGGG 
AACAGTGTGG CCGGCAGCCC TTTGCCCCTC ACGACAGGAA ACGAGAACCT AGACGTAGCC 
GTCGTCATTC ATCTGCAATA CTGCAATCAT CTCATTCAGT TGTTGACTTC AGGAGGAAGC 
CAGTTACAGC ATAAGACATA CCTGCACAAA CTAGCAACTC AAACTCTGCT GTTAGAGGAG 
CTCAGTGAGA GGAACTTTGA CAGACCGAGC AGCTGCACTT CAGTGCCTGA TGTGCTGCCC 
GGGCTGGTGG AGCGGCCGGC GCTGATGTCT CTATGGTCCG ACTGCAGCGG TGGGCTTTTC 
CACACCACAC TAGACCGAGT ATTAAAGCAC ATGCATCTCA GCTTTGCCTT AGGACTGCAA 
CAGGTTCTGC CTCACTCTCC TGACTCAGTG ATCAAGATGG TGGTGAATGA GATGATGGAC 
AGGAGTGAAC TGGCCTCCTC CTCAACTTCT CCAGCACAGG ACGTCATCAC TGTGTTTCAG 
TTCCACCGCT ACATTGTTGA ACACTGTATC ACAGACATGG AGCAACACCT GCTGGATTTA 
GCCAAAGAGG TCATGCTTGA AGAAGCCCTG AGCTGTGGAG ATCCAGAACG CTCTCTGAAG 
GAACTAAAGA AAGTGTCCAT CACGTGTCTT CAGCCCCGTT CTCAAACTCT ATTGGCTGTG 
GCTGGTCTGC TGACCACTGA TGACCCTGAA GTGATGAAGG CCACCACAGA CTTCCTCTCC 
TCTGCTGCCT CACATCAGCC CTTCAGGTCA AAGGCTGTTG ATTGTTACAC ACAAGCCTTA 
TCAGAAAGTG AGTTACACAC ACAGAGAGCT GCATGTGCTG CTCTGAGCTG CCTTCAGGCT 
GTGGAGAGCA TTGATGCTGT CATTTTACTG TGTGACTCTG TGGATGAAGA GCTCCGGCAG 
ATTGCTATTG AAACCCTCCT CACTTTCGGT GAGGATGGGC GGCTGGCATA CGAGCAACTG 
GACACCGTCC CGAGGGAGAT GGTGCGGCTG GGCACTCGGC GTGGAAATGC GGTGACCACT 
TCATTTTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Fam65b is important for formation of the HDAC6-dysferlin protein complex during myogenic cell differentiation.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology28(7)2955-69(2014 Jul)
Balasubramanian A,Kawahara G,Gupta VA,Rozkalne A,Beauvais A,Kunkel LM,Gussoni E

FAM65B is a membrane-associated protein of hair cell stereocilia required for hearing.
Proceedings of the National Academy of Sciences of the United States of America111(27)9864-8(2014 Jul)
Diaz-Horta O,Subasioglu-Uzak A,Grati M,DeSmidt A,Foster J 2nd,Cao L,Bademci G,Tokgoz-Yilmaz S,Duman D,Cengiz FB,Abad C,Mittal R,Blanton S,Liu XZ,Farooq A,Walz K,Lu Z,Tekin M

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FAM65B cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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