si:dkey-21e5.1 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	si:dkey-21e5.1
Entrez Gene ID	563670
Full Name	si:dkey-21e5.1
General protein information	Preferred Names si:dkey-21e5.1 Names potassium channel subfamily T member 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017356387.1	XP_017211876.1	potassium channel subfamily T member 1
XM_021476673.1	XP_021332348.1	potassium channel subfamily T member 1 isoform X1
XM_021476675.1	XP_021332350.1	potassium channel subfamily T member 1 isoform X3
XM_021476676.1	XP_021332351.1	potassium channel subfamily T member 1 isoform X4
XM_021476678.1	XP_021332353.1	potassium channel subfamily T member 1 isoform X6
XM_021476674.1	XP_021332349.1	potassium channel subfamily T member 1 isoform X2
XM_021476677.1	XP_021332352.1	potassium channel subfamily T member 1 isoform X5

Pathways from BioSystems
Gene Ontology

The following si:dkey-21e5.1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the si:dkey-21e5.1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa142854	XM_021476673.1 Latest version!	Danio rerio si:dkey-21e5.1 (si:dkey-21e5.1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa142855	XM_021476675.1 Latest version!	Danio rerio si:dkey-21e5.1 (si:dkey-21e5.1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa142856	XM_021476676.1 Latest version!	Danio rerio si:dkey-21e5.1 (si:dkey-21e5.1), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa142857	XM_021476677.1 Latest version!	Danio rerio si:dkey-21e5.1 (si:dkey-21e5.1), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa142858	XM_021476678.1 Latest version!	Danio rerio si:dkey-21e5.1 (si:dkey-21e5.1), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa44133	XM_021476674.1 Latest version!	Danio rerio si:dkey-21e5.1 (si:dkey-21e5.1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa59308	XM_017356387.1 Latest version!	Danio rerio si:dkey-21e5.1 (si:dkey-21e5.1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

si:dkey-21e5.1 ( XM_021476673.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332348.1 Danio rerio si:dkey-21e5.1 (si:dkey-21e5.1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa142854
Clone ID Related Accession (Same CDS sequence)	XM_021476673.1
Accession Version	XM_021476673.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3786bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	potassium channel subfamily T member 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGAC CGCGCAGTCA GTCTCAGGCA GAGCCAAGCA TGACTCCGCC ACGACGCTCT 
TCCACTGCTG CTGACAGGTT GCCTACGGAG ACCGTTCAGA GAAATAACAG CAGTAATACT 
GGTGTTATTC TGGATATTTC CACGCTGAAA TTGGCAGATG TGGACTCTGA GGTCCCTCCT 
CTACCTCCAC GATACCGCTT TCGAGATCTG CTGCTTGGGG ATCAGTCATT TCAGAACGAT 
GACAGGGTGC AGGTGGAGTT TTACGTAAAC GAAAACACAT TTAAGGAGAG GTTGAAGCTC 
TTCTTCATCA AAAACCAAAG GTCAAGTCTC AGAATACGCC TCTTTAACCT CTCCTTAAAG 
TTGTTAACAT GCCTGCTCTA CATAATCAGA GTCTCACTTG ATGATCCAAC TAACCAGGTC 
ACATGTGCAC GGAAACACTG CGCAAACATC ACAGATGCTC TGTGCAGCAA AGCCTGTGCC 
AACTATACAG CAAACCTGAC CGCAGATCCC ACACAAATAA ACTGGGAGTT AATATTTTGG 
GTGAATAGAA GAGTTCCTGT ATGGGCGATA CAGGTGACAG TGGCCTTAAT TAGCTTCTTG 
CAAGCGATGC TCCTGGTGTA TCTCAGCTAC AAGGGCAACA TTTGGGAACA GATTTTCCAA 
ATCTCCTTCA TCATTGAGAT GATCAATACA GTGCCATTCA TAATTACAAT CTTTTGGCCT 
CCGCTTAGGA ACATATTTGT TCCTGTATTT CTTAACTGTT GGCTTGCCAA ATCAGCGCTG 
GAGAATATGA TTAATGATCT CCATCGTGCC ATCCAGCGCA CACACTCTGC CATGTTCAAC 
CAGGTCCTCA TCCTGATCTG TACACTGCTC TGTCTGGTCT TCACCGGGGC TTGTGGGATT 
CAGCACCTGG AGCGAGCAGG GAAGAATCTG ACACTCTTTG ACGCTTTATA TTTCTGCATC 
GTCACCTTCT CCACTGTTGG ATATGGAGAT GTGACGCCCA GAATCTGGCC CTCTCAGCTG 
CTGGTGGTCA TCATGATCTG TGTGGCGCTG GTGGTGCTGC CATTACAGTT CGAGGAGTTG 
GCGTATCTAT GGATGGAGAG GCAGAAGTCA GGTGGAAACT ACAGCAGACA CCGCGCTCAG 
ACAGAGAAAC ACGTGGTGCT CTGCGTCACC TCACTCAAAA TAGACCTGCT CATGGATTTC 
CTCAATGAGT TCTATGCCCA CCCGAGACTA CAGGACTATT ATGTGGTGAT CCTCTGTCCC 
ACGGAGATGG ACATCCAGGT GCGGCGAATA CTGCAGATCC CACTGTGGTC TCAGCGGGTC 
ATCTACCTCC AAGGCTCTGC ACTGAAGGAC CAAGACCTCA TGAGGGCCAA GATGGATGAT 
GCGGAGGCCT GCTTCATCCT CAGCAGCAGA AATGAAGTGG ACCGCACCGC TGCTGATCAT 
CAGACCATTC TGCGAGCCTG GGCTGTGAAG GATTTTGCTC CAAACTGTCC TCTTTATGTC 
CAGATCCTCA AACCCGAGAA CAAATTTCAT GTCAAATTTG CAGATCACGT GGTGTGTGAA 
GAGGAGTTCA AGTACGCCAT GCTGGCCCTG AACTGTGTGT GTCCCGCCAC CTCCACACTG 
GTCACCTTGT TGGTGCACAC GTCTCGAGGA CAAGAAGGTC AGACGTCTCC AGAACAGTGG 
CAGAGGACTT ACGGTCGCTG CTCGGGGAAT GAAGTTTATC ACATCCGTTT GATGGACAGC 
AAGTTTTTTG GAGAATATGA TGGGAAGAGT TTCACTTATG CCTCCTTCCA CGCACACAAG 
AAGTATGGTG TGTGTCTAAT CGGCACAAAG AGAGAAGACA ACAAGAGCAT TCTCCTGAAC 
CCCGGCCCTC GCCACATCAT GTCCGCCACA GACACCTGCT ACTACATCAA CATCACCAAA 
GAGGAGAACT CGGCCTTCAT CTTCAAGCAG GAGGAGAAGC ACAGTAAAGG CTTATCAGCC 
GCCGGCGTGT ACGACGCTCC GTCTCGCCTC CCAGTGCAAA GCATCATTGC CAGCATGGTT 
GATCAGTCTA CTTCATATGG AACTGTGGCC ATTGACCTGC AGAACCCGGA TCCACCGGCT 
GACAGCAATA AACTGGCTCT GCCCACGGAG AACGGAGCGG GCAGCCGCAG ACCCAGCATT 
GCCCCTGTGC TGGAGATCGC AGACTCCTCT GCCATCCTGC CCTGTGATCT CCTCAACGAC 
CCCTCAGAAG ACGAGACCAA CCAATCAGAT GAGGATGGAG CGCCGGCCAT AGACTGTGTT 
AAAGGCTATC CTCCAAACTC CCCTTACATT GGAAGCTCTC CAACACTTTG CCACCTTCTT 
CAACAGAAGG CACCGTTTTG CTGTCTACGA CTTGACAAGG GTTGTCGACA CAATAGTTTC 
GAAGACGCCA AGGCGTATGG TTTCAAGAAC AAGTTGATTA TCGTTTCTGC TGAGACAGCG 
GGAAATGGCT TGTACAACTT CATAGTGCCG TTACGTGCCT ATTACCGACC GCGGAAGGAG 
CTGAATCCCA TTGTGCTGCT GCTGGATAAT CCGCCTGATA ATCATTTCCT TGAGGCCATC 
TGCTGTTTCC CTATGGTGTA CTACATGGCG GGAACCATAG ACAATTTGGA TAACCTGCTG 
CAGTGTGGCA TCATCTACGC TGATAACCTG GTGGTGGTGG ATAAGGAGAG CACTATGAGC 
GCAGAGGAAG ACTACATGGC AGACGCTAAA ACCATAGTCA ATGTGCAGAC CATGTTCAGG 
TTGTTTCCTA GTCTCAGCAT AATCACTGAG CTAACTCATC CGTCTAATAT GAGGTTCATG 
CAGTTCCGTG CCAAAGACTG CTACTCCCTG GCTCTCTCCA AACTCGAAAA GATAGAGCGA 
GATAAGGGAT CGAATCTGGC GTTCATGTTC CGCCTGCCGT TCGCTGCAGG CAGAGTCTTC 
AGTATCAGCA TGCTGGATAC TCTGCTTTAC CAGTCGTTTG TGAAGGACTA TATGATTCTC 
ATTGCGCGGC TCCTCCTGGG TTTAGACACC ACCCCGGGCT CAGGATACCT CTGTGCTATG 
AAGGTAACAG AAGAGGACTT ATGGATTCGA ACGTATGGGA GACTCTTCCA GAAGCTGTGT 
TCCTCCAGCG CAGAGATTCC CATCGGGATT TACCGCACCG AATCCCACAT GTTCTCCTCT 
TCAGAGTCTC AGGTGTCTGT GGAGGATTGC GAGGACCAGC GGGACCACAG GGAATCCTGG 
AAAGAGAAAA CTGCCCATAG GAACTCCACA GGGAGCGACC AATCAGAGCA TCCGCTCCTG 
CGCAAGAAAA GCATGCAGTG GGCGAGACGC CTAAGCAGAA AAAACACCAA ACCTCCCAGT 
CGAGCTGAAC GCATCAATCA ACAGCGCCTG AACCTGTACC GGCGCTCCGA GAGGCAGGAG 
CTGTCAGAGC TGGTGAAGAA CCGCATGAAG CATTTAGGCC TGCCCACTGT GGGATACGAG 
GACGTAGCTA ATTTAACCGC AAGCGATGTG ATGAACCGAG TAAATCTGGG ATATTTGCAA 
GAGGGTACTT ACATGACAGA GGGTACTGCA GTGGCTCAAA CAGATGAAAT GAATGACAAT 
CAGAACACGT TGTCATATGT GCTGATAAAC CCTCCGCCGG ACACCATTCT GGAGCTCAAT 
GACATTGTCT ACATCATCCG TTCTGATCCA CTGGCACATG TCCCTGAAAA TCTCCCAGGG 
GCTCCAGCTG GAGCGAAGAT ACGCCAAGAC TATCGGCCCG AGACAAGGGA TGAGACGCAT 
CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332348.1
CDS	703..4488
Translation	MMGPRSQSQAEPSMTPPRRSSTAADRLPTETVQRNNSSNTGVILDISTLKLADVDSEVPPLPPRYRFRDLLLGDQSFQNDDRVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNLSLKLLTCLLYIIRVSLDDPTNQVTCARKHCANITDALCSKACANYTANLTADPTQINWELIFWVNRRVPVWAIQVTVALISFLQAMLLVYLSYKGNIWEQIFQISFIIEMINTVPFIITIFWPPLRNIFVPVFLNCWLAKSALENMINDLHRAIQRTHSAMFNQVLILICTLLCLVFTGACGIQHLERAGKNLTLFDALYFCIVTFSTVGYGDVTPRIWPSQLLVVIMICVALVVLPLQFEELAYLWMERQKSGGNYSRHRAQTEKHVVLCVTSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRILQIPLWSQRVIYLQGSALKDQDLMRAKMDDAEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEEFKYAMLALNCVCPATSTLVTLLVHTSRGQEGQTSPEQWQRTYGRCSGNEVYHIRLMDSKFFGEYDGKSFTYASFHAHKKYGVCLIGTKREDNKSILLNPGPRHIMSATDTCYYINITKEENSAFIFKQEEKHSKGLSAAGVYDAPSRLPVQSIIASMVDQSTSYGTVAIDLQNPDPPADSNKLALPTENGAGSRRPSIAPVLEIADSSAILPCDLLNDPSEDETNQSDEDGAPAIDCVKGYPPNSPYIGSSPTLCHLLQQKAPFCCLRLDKGCRHNSFEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRKELNPIVLLLDNPPDNHFLEAICCFPMVYYMAGTIDNLDNLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSIITELTHPSNMRFMQFRAKDCYSLALSKLEKIERDKGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMILIARLLLGLDTTPGSGYLCAMKVTEEDLWIRTYGRLFQKLCSSSAEIPIGIYRTESHMFSSSESQVSVEDCEDQRDHRESWKEKTAHRNSTGSDQSEHPLLRKKSMQWARRLSRKNTKPPSRAERINQQRLNLYRRSERQELSELVKNRMKHLGLPTVGYEDVANLTASDVMNRVNLGYLQEGTYMTEGTAVAQTDEMNDNQNTLSYVLINPPPDTILELNDIVYIIRSDPLAHVPENLPGAPAGAKIRQDYRPETRDETHL

Target ORF information:

RefSeq Version	XM_021476673.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:dkey-21e5.1 (si:dkey-21e5.1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476673.1

ORF Insert Sequence:

ATGATGGGAC CGCGCAGTCA GTCTCAGGCA GAGCCAAGCA TGACTCCGCC ACGACGCTCT 
TCCACTGCTG CTGACAGGTT GCCTACGGAG ACCGTTCAGA GAAATAACAG CAGTAATACT 
GGTGTTATTC TGGATATTTC CACGCTGAAA TTGGCAGATG TGGACTCTGA GGTCCCTCCT 
CTACCTCCAC GATACCGCTT TCGAGATCTG CTGCTTGGGG ATCAGTCATT TCAGAACGAT 
GACAGGGTGC AGGTGGAGTT TTACGTAAAC GAAAACACAT TTAAGGAGAG GTTGAAGCTC 
TTCTTCATCA AAAACCAAAG GTCAAGTCTC AGAATACGCC TCTTTAACCT CTCCTTAAAG 
TTGTTAACAT GCCTGCTCTA CATAATCAGA GTCTCACTTG ATGATCCAAC TAACCAGGTC 
ACATGTGCAC GGAAACACTG CGCAAACATC ACAGATGCTC TGTGCAGCAA AGCCTGTGCC 
AACTATACAG CAAACCTGAC CGCAGATCCC ACACAAATAA ACTGGGAGTT AATATTTTGG 
GTGAATAGAA GAGTTCCTGT ATGGGCGATA CAGGTGACAG TGGCCTTAAT TAGCTTCTTG 
CAAGCGATGC TCCTGGTGTA TCTCAGCTAC AAGGGCAACA TTTGGGAACA GATTTTCCAA 
ATCTCCTTCA TCATTGAGAT GATCAATACA GTGCCATTCA TAATTACAAT CTTTTGGCCT 
CCGCTTAGGA ACATATTTGT TCCTGTATTT CTTAACTGTT GGCTTGCCAA ATCAGCGCTG 
GAGAATATGA TTAATGATCT CCATCGTGCC ATCCAGCGCA CACACTCTGC CATGTTCAAC 
CAGGTCCTCA TCCTGATCTG TACACTGCTC TGTCTGGTCT TCACCGGGGC TTGTGGGATT 
CAGCACCTGG AGCGAGCAGG GAAGAATCTG ACACTCTTTG ACGCTTTATA TTTCTGCATC 
GTCACCTTCT CCACTGTTGG ATATGGAGAT GTGACGCCCA GAATCTGGCC CTCTCAGCTG 
CTGGTGGTCA TCATGATCTG TGTGGCGCTG GTGGTGCTGC CATTACAGTT CGAGGAGTTG 
GCGTATCTAT GGATGGAGAG GCAGAAGTCA GGTGGAAACT ACAGCAGACA CCGCGCTCAG 
ACAGAGAAAC ACGTGGTGCT CTGCGTCACC TCACTCAAAA TAGACCTGCT CATGGATTTC 
CTCAATGAGT TCTATGCCCA CCCGAGACTA CAGGACTATT ATGTGGTGAT CCTCTGTCCC 
ACGGAGATGG ACATCCAGGT GCGGCGAATA CTGCAGATCC CACTGTGGTC TCAGCGGGTC 
ATCTACCTCC AAGGCTCTGC ACTGAAGGAC CAAGACCTCA TGAGGGCCAA GATGGATGAT 
GCGGAGGCCT GCTTCATCCT CAGCAGCAGA AATGAAGTGG ACCGCACCGC TGCTGATCAT 
CAGACCATTC TGCGAGCCTG GGCTGTGAAG GATTTTGCTC CAAACTGTCC TCTTTATGTC 
CAGATCCTCA AACCCGAGAA CAAATTTCAT GTCAAATTTG CAGATCACGT GGTGTGTGAA 
GAGGAGTTCA AGTACGCCAT GCTGGCCCTG AACTGTGTGT GTCCCGCCAC CTCCACACTG 
GTCACCTTGT TGGTGCACAC GTCTCGAGGA CAAGAAGGTC AGACGTCTCC AGAACAGTGG 
CAGAGGACTT ACGGTCGCTG CTCGGGGAAT GAAGTTTATC ACATCCGTTT GATGGACAGC 
AAGTTTTTTG GAGAATATGA TGGGAAGAGT TTCACTTATG CCTCCTTCCA CGCACACAAG 
AAGTATGGTG TGTGTCTAAT CGGCACAAAG AGAGAAGACA ACAAGAGCAT TCTCCTGAAC 
CCCGGCCCTC GCCACATCAT GTCCGCCACA GACACCTGCT ACTACATCAA CATCACCAAA 
GAGGAGAACT CGGCCTTCAT CTTCAAGCAG GAGGAGAAGC ACAGTAAAGG CTTATCAGCC 
GCCGGCGTGT ACGACGCTCC GTCTCGCCTC CCAGTGCAAA GCATCATTGC CAGCATGGTT 
GATCAGTCTA CTTCATATGG AACTGTGGCC ATTGACCTGC AGAACCCGGA TCCACCGGCT 
GACAGCAATA AACTGGCTCT GCCCACGGAG AACGGAGCGG GCAGCCGCAG ACCCAGCATT 
GCCCCTGTGC TGGAGATCGC AGACTCCTCT GCCATCCTGC CCTGTGATCT CCTCAACGAC 
CCCTCAGAAG ACGAGACCAA CCAATCAGAT GAGGATGGAG CGCCGGCCAT AGACTGTGTT 
AAAGGCTATC CTCCAAACTC CCCTTACATT GGAAGCTCTC CAACACTTTG CCACCTTCTT 
CAACAGAAGG CACCGTTTTG CTGTCTACGA CTTGACAAGG GTTGTCGACA CAATAGTTTC 
GAAGACGCCA AGGCGTATGG TTTCAAGAAC AAGTTGATTA TCGTTTCTGC TGAGACAGCG 
GGAAATGGCT TGTACAACTT CATAGTGCCG TTACGTGCCT ATTACCGACC GCGGAAGGAG 
CTGAATCCCA TTGTGCTGCT GCTGGATAAT CCGCCTGATA ATCATTTCCT TGAGGCCATC 
TGCTGTTTCC CTATGGTGTA CTACATGGCG GGAACCATAG ACAATTTGGA TAACCTGCTG 
CAGTGTGGCA TCATCTACGC TGATAACCTG GTGGTGGTGG ATAAGGAGAG CACTATGAGC 
GCAGAGGAAG ACTACATGGC AGACGCTAAA ACCATAGTCA ATGTGCAGAC CATGTTCAGG 
TTGTTTCCTA GTCTCAGCAT AATCACTGAG CTAACTCATC CGTCTAATAT GAGGTTCATG 
CAGTTCCGTG CCAAAGACTG CTACTCCCTG GCTCTCTCCA AACTCGAAAA GATAGAGCGA 
GATAAGGGAT CGAATCTGGC GTTCATGTTC CGCCTGCCGT TCGCTGCAGG CAGAGTCTTC 
AGTATCAGCA TGCTGGATAC TCTGCTTTAC CAGTCGTTTG TGAAGGACTA TATGATTCTC 
ATTGCGCGGC TCCTCCTGGG TTTAGACACC ACCCCGGGCT CAGGATACCT CTGTGCTATG 
AAGGTAACAG AAGAGGACTT ATGGATTCGA ACGTATGGGA GACTCTTCCA GAAGCTGTGT 
TCCTCCAGCG CAGAGATTCC CATCGGGATT TACCGCACCG AATCCCACAT GTTCTCCTCT 
TCAGAGTCTC AGGTGTCTGT GGAGGATTGC GAGGACCAGC GGGACCACAG GGAATCCTGG 
AAAGAGAAAA CTGCCCATAG GAACTCCACA GGGAGCGACC AATCAGAGCA TCCGCTCCTG 
CGCAAGAAAA GCATGCAGTG GGCGAGACGC CTAAGCAGAA AAAACACCAA ACCTCCCAGT 
CGAGCTGAAC GCATCAATCA ACAGCGCCTG AACCTGTACC GGCGCTCCGA GAGGCAGGAG 
CTGTCAGAGC TGGTGAAGAA CCGCATGAAG CATTTAGGCC TGCCCACTGT GGGATACGAG 
GACGTAGCTA ATTTAACCGC AAGCGATGTG ATGAACCGAG TAAATCTGGG ATATTTGCAA 
GAGGGTACTT ACATGACAGA GGGTACTGCA GTGGCTCAAA CAGATGAAAT GAATGACAAT 
CAGAACACGT TGTCATATGT GCTGATAAAC CCTCCGCCGG ACACCATTCT GGAGCTCAAT 
GACATTGTCT ACATCATCCG TTCTGATCCA CTGGCACATG TCCCTGAAAA TCTCCCAGGG 
GCTCCAGCTG GAGCGAAGAT ACGCCAAGAC TATCGGCCCG AGACAAGGGA TGAGACGCAT 
CTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:dkey-21e5.1 ( XM_021476675.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332350.1 Danio rerio si:dkey-21e5.1 (si:dkey-21e5.1), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa142855
Clone ID Related Accession (Same CDS sequence)	XM_021476675.1
Accession Version	XM_021476675.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3765bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	potassium channel subfamily T member 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGAC CGCGCAGTCA GTCTCAGGCA GAGCCAAGCA TGACTCCGCC ACGACGCTCT 
TCCACTGCTG CTGACAGGTT GCCTACGGAG ACCGTTCAGA GAAATAACAG CAGTAATACT 
GGTGTTATTC TGGATATTTC CACGCTGAAA TTGGCAGATG TGGACTCTGA GGTCCCTCCT 
CTACCTCCAC GATACCGCTT TCGAGATCTG CTGCTTGGGG ATCAGTCATT TCAGAACGAT 
GACAGGGTGC AGGTGGAGTT TTACGTAAAC GAAAACACAT TTAAGGAGAG GTTGAAGCTC 
TTCTTCATCA AAAACCAAAG GTCAAGTCTC AGAATACGCC TCTTTAACCT CTCCTTAAAG 
TTGTTAACAT GCCTGCTCTA CATAATCAGA GTCTCACTTG ATGATCCAAC TAACCAGGTC 
ACATGTGCAC GGAAACACTG CGCAAACATC ACAGATGCTC TGTGCAGCAA AGCCTGTGCC 
AACTATACAG CAAACCTGAC CGCAGATCCC ACACAAATAA ACTGGGAGTT AATATTTTGG 
GTGAATAGAA GAGTTCCTGT ATGGGCGATA CAGGTGACAG TGGCCTTAAT TAGCTTCTTG 
CAAGCGATGC TCCTGGTGTA TCTCAGCTAC AAGGGCAACA TTTGGGAACA GATTTTCCAA 
ATCTCCTTCA TCATTGAGAT GATCAATACA GTGCCATTCA TAATTACAAT CTTTTGGCCT 
CCGCTTAGGA ACATATTTGT TCCTGTATTT CTTAACTGTT GGCTTGCCAA ATCAGCGCTG 
GAGAATATGA TTAATGATCT CCATCGTGCC ATCCAGCGCA CACACTCTGC CATGTTCAAC 
CAGGTCCTCA TCCTGATCTG TACACTGCTC TGTCTGGTCT TCACCGGGGC TTGTGGGATT 
CAGCACCTGG AGCGAGCAGG GAAGAATCTG ACACTCTTTG ACGCTTTATA TTTCTGCATC 
GTCACCTTCT CCACTGTTGG ATATGGAGAT GTGACGCCCA GAATCTGGCC CTCTCAGCTG 
CTGGTGGTCA TCATGATCTG TGTGGCGCTG GTGGTGCTGC CATTACAGTT CGAGGAGTTG 
GCGTATCTAT GGATGGAGAG GCAGAAGTCA GGTGGAAACT ACAGCAGACA CCGCGCTCAG 
ACAGAGAAAC ACGTGGTGCT CTGCGTCACC TCACTCAAAA TAGACCTGCT CATGGATTTC 
CTCAATGAGT TCTATGCCCA CCCGAGACTA CAGGACTATT ATGTGGTGAT CCTCTGTCCC 
ACGGAGATGG ACATCCAGGT GCGGCGAATA CTGCAGATCC CACTGTGGTC TCAGCGGGTC 
ATCTACCTCC AAGGCTCTGC ACTGAAGGAC CAAGACCTCA TGAGGGCCAA GATGGATGAT 
GCGGAGGCCT GCTTCATCCT CAGCAGCAGA AATGAAGTGG ACCGCACCGC TGCTGATCAT 
CAGACCATTC TGCGAGCCTG GGCTGTGAAG GATTTTGCTC CAAACTGTCC TCTTTATGTC 
CAGATCCTCA AACCCGAGAA CAAATTTCAT GTCAAATTTG CAGATCACGT GGTGTGTGAA 
GAGGAGTTCA AGTACGCCAT GCTGGCCCTG AACTGTGTGT GTCCCGCCAC CTCCACACTG 
GTCACCTTGT TGGTGCACAC GTCTCGAGGA CAAGAAGGTC AGACGTCTCC AGAACAGTGG 
CAGAGGACTT ACGGTCGCTG CTCGGGGAAT GAAGTTTATC ACATCCGTTT GATGGACAGC 
AAGTTTTTTG GAGAATATGA TGGGAAGAGT TTCACTTATG CCTCCTTCCA CGCACACAAG 
AAGTATGGTG TGTGTCTAAT CGGCACAAAG AGAGAAGACA ACAAGAGCAT TCTCCTGAAC 
CCCGGCCCTC GCCACATCAT GTCCGCCACA GACACCTGCT ACTACATCAA CATCACCAAA 
GAGGAGAACT CGGCCTTCAT CTTCAAGCAG GAGGAGAAGC ACAGTAAAGG CTTATCAGCC 
GCCGGCGTGT ACGACGCTCC GTCTCGCCTC CCAGTGCAAA GCATCATTGC CAGCATGGGA 
ACTGTGGCCA TTGACCTGCA GAACCCGGAT CCACCGGCTG ACAGCAATAA ACTGGCTCTG 
CCCACGGAGA ACGGAGCGGG CAGCCGCAGA CCCAGCATTG CCCCTGTGCT GGAGATCGCA 
GACTCCTCTG CCATCCTGCC CTGTGATCTC CTCAACGACC CCTCAGAAGA CGAGACCAAC 
CAATCAGATG AGGATGGAGC GCCGGCCATA GACTGTGTTA AAGGCTATCC TCCAAACTCC 
CCTTACATTG GAAGCTCTCC AACACTTTGC CACCTTCTTC AACAGAAGGC ACCGTTTTGC 
TGTCTACGAC TTGACAAGGG TTGTCGACAC AATAGTTTCG AAGACGCCAA GGCGTATGGT 
TTCAAGAACA AGTTGATTAT CGTTTCTGCT GAGACAGCGG GAAATGGCTT GTACAACTTC 
ATAGTGCCGT TACGTGCCTA TTACCGACCG CGGAAGGAGC TGAATCCCAT TGTGCTGCTG 
CTGGATAATC CGCCTGATAA TCATTTCCTT GAGGCCATCT GCTGTTTCCC TATGGTGTAC 
TACATGGCGG GAACCATAGA CAATTTGGAT AACCTGCTGC AGTGTGGCAT CATCTACGCT 
GATAACCTGG TGGTGGTGGA TAAGGAGAGC ACTATGAGCG CAGAGGAAGA CTACATGGCA 
GACGCTAAAA CCATAGTCAA TGTGCAGACC ATGTTCAGGT TGTTTCCTAG TCTCAGCATA 
ATCACTGAGC TAACTCATCC GTCTAATATG AGGTTCATGC AGTTCCGTGC CAAAGACTGC 
TACTCCCTGG CTCTCTCCAA ACTCGAAAAG ATAGAGCGAG ATAAGGGATC GAATCTGGCG 
TTCATGTTCC GCCTGCCGTT CGCTGCAGGC AGAGTCTTCA GTATCAGCAT GCTGGATACT 
CTGCTTTACC AGTCGTTTGT GAAGGACTAT ATGATTCTCA TTGCGCGGCT CCTCCTGGGT 
TTAGACACCA CCCCGGGCTC AGGATACCTC TGTGCTATGA AGGTAACAGA AGAGGACTTA 
TGGATTCGAA CGTATGGGAG ACTCTTCCAG AAGCTGTGTT CCTCCAGCGC AGAGATTCCC 
ATCGGGATTT ACCGCACCGA ATCCCACATG TTCTCCTCTT CAGAGTCTCA GGTGTCTGTG 
GAGGATTGCG AGGACCAGCG GGACCACAGG GAATCCTGGA AAGAGAAAAC TGCCCATAGG 
AACTCCACAG GGAGCGACCA ATCAGAGCAT CCGCTCCTGC GCAAGAAAAG CATGCAGTGG 
GCGAGACGCC TAAGCAGAAA AAACACCAAA CCTCCCAGTC GAGCTGAACG CATCAATCAA 
CAGCGCCTGA ACCTGTACCG GCGCTCCGAG AGGCAGGAGC TGTCAGAGCT GGTGAAGAAC 
CGCATGAAGC ATTTAGGCCT GCCCACTGTG GGATACGAGG ACGTAGCTAA TTTAACCGCA 
AGCGATGTGA TGAACCGAGT AAATCTGGGA TATTTGCAAG AGGGTACTTA CATGACAGAG 
GGTACTGCAG TGGCTCAAAC AGATGAAATG AATGACAATC AGAACACGTT GTCATATGTG 
CTGATAAACC CTCCGCCGGA CACCATTCTG GAGCTCAATG ACATTGTCTA CATCATCCGT 
TCTGATCCAC TGGCACATGT CCCTGAAAAT CTCCCAGGGG CTCCAGCTGG AGCGAAGATA 
CGCCAAGACT ATCGGCCCGA GACAAGGGAT GAGACGCATC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332350.1
CDS	703..4467
Translation	MMGPRSQSQAEPSMTPPRRSSTAADRLPTETVQRNNSSNTGVILDISTLKLADVDSEVPPLPPRYRFRDLLLGDQSFQNDDRVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNLSLKLLTCLLYIIRVSLDDPTNQVTCARKHCANITDALCSKACANYTANLTADPTQINWELIFWVNRRVPVWAIQVTVALISFLQAMLLVYLSYKGNIWEQIFQISFIIEMINTVPFIITIFWPPLRNIFVPVFLNCWLAKSALENMINDLHRAIQRTHSAMFNQVLILICTLLCLVFTGACGIQHLERAGKNLTLFDALYFCIVTFSTVGYGDVTPRIWPSQLLVVIMICVALVVLPLQFEELAYLWMERQKSGGNYSRHRAQTEKHVVLCVTSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRILQIPLWSQRVIYLQGSALKDQDLMRAKMDDAEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEEFKYAMLALNCVCPATSTLVTLLVHTSRGQEGQTSPEQWQRTYGRCSGNEVYHIRLMDSKFFGEYDGKSFTYASFHAHKKYGVCLIGTKREDNKSILLNPGPRHIMSATDTCYYINITKEENSAFIFKQEEKHSKGLSAAGVYDAPSRLPVQSIIASMGTVAIDLQNPDPPADSNKLALPTENGAGSRRPSIAPVLEIADSSAILPCDLLNDPSEDETNQSDEDGAPAIDCVKGYPPNSPYIGSSPTLCHLLQQKAPFCCLRLDKGCRHNSFEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRKELNPIVLLLDNPPDNHFLEAICCFPMVYYMAGTIDNLDNLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSIITELTHPSNMRFMQFRAKDCYSLALSKLEKIERDKGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMILIARLLLGLDTTPGSGYLCAMKVTEEDLWIRTYGRLFQKLCSSSAEIPIGIYRTESHMFSSSESQVSVEDCEDQRDHRESWKEKTAHRNSTGSDQSEHPLLRKKSMQWARRLSRKNTKPPSRAERINQQRLNLYRRSERQELSELVKNRMKHLGLPTVGYEDVANLTASDVMNRVNLGYLQEGTYMTEGTAVAQTDEMNDNQNTLSYVLINPPPDTILELNDIVYIIRSDPLAHVPENLPGAPAGAKIRQDYRPETRDETHL

Target ORF information:

RefSeq Version	XM_021476675.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:dkey-21e5.1 (si:dkey-21e5.1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476675.1

ORF Insert Sequence:

ATGATGGGAC CGCGCAGTCA GTCTCAGGCA GAGCCAAGCA TGACTCCGCC ACGACGCTCT 
TCCACTGCTG CTGACAGGTT GCCTACGGAG ACCGTTCAGA GAAATAACAG CAGTAATACT 
GGTGTTATTC TGGATATTTC CACGCTGAAA TTGGCAGATG TGGACTCTGA GGTCCCTCCT 
CTACCTCCAC GATACCGCTT TCGAGATCTG CTGCTTGGGG ATCAGTCATT TCAGAACGAT 
GACAGGGTGC AGGTGGAGTT TTACGTAAAC GAAAACACAT TTAAGGAGAG GTTGAAGCTC 
TTCTTCATCA AAAACCAAAG GTCAAGTCTC AGAATACGCC TCTTTAACCT CTCCTTAAAG 
TTGTTAACAT GCCTGCTCTA CATAATCAGA GTCTCACTTG ATGATCCAAC TAACCAGGTC 
ACATGTGCAC GGAAACACTG CGCAAACATC ACAGATGCTC TGTGCAGCAA AGCCTGTGCC 
AACTATACAG CAAACCTGAC CGCAGATCCC ACACAAATAA ACTGGGAGTT AATATTTTGG 
GTGAATAGAA GAGTTCCTGT ATGGGCGATA CAGGTGACAG TGGCCTTAAT TAGCTTCTTG 
CAAGCGATGC TCCTGGTGTA TCTCAGCTAC AAGGGCAACA TTTGGGAACA GATTTTCCAA 
ATCTCCTTCA TCATTGAGAT GATCAATACA GTGCCATTCA TAATTACAAT CTTTTGGCCT 
CCGCTTAGGA ACATATTTGT TCCTGTATTT CTTAACTGTT GGCTTGCCAA ATCAGCGCTG 
GAGAATATGA TTAATGATCT CCATCGTGCC ATCCAGCGCA CACACTCTGC CATGTTCAAC 
CAGGTCCTCA TCCTGATCTG TACACTGCTC TGTCTGGTCT TCACCGGGGC TTGTGGGATT 
CAGCACCTGG AGCGAGCAGG GAAGAATCTG ACACTCTTTG ACGCTTTATA TTTCTGCATC 
GTCACCTTCT CCACTGTTGG ATATGGAGAT GTGACGCCCA GAATCTGGCC CTCTCAGCTG 
CTGGTGGTCA TCATGATCTG TGTGGCGCTG GTGGTGCTGC CATTACAGTT CGAGGAGTTG 
GCGTATCTAT GGATGGAGAG GCAGAAGTCA GGTGGAAACT ACAGCAGACA CCGCGCTCAG 
ACAGAGAAAC ACGTGGTGCT CTGCGTCACC TCACTCAAAA TAGACCTGCT CATGGATTTC 
CTCAATGAGT TCTATGCCCA CCCGAGACTA CAGGACTATT ATGTGGTGAT CCTCTGTCCC 
ACGGAGATGG ACATCCAGGT GCGGCGAATA CTGCAGATCC CACTGTGGTC TCAGCGGGTC 
ATCTACCTCC AAGGCTCTGC ACTGAAGGAC CAAGACCTCA TGAGGGCCAA GATGGATGAT 
GCGGAGGCCT GCTTCATCCT CAGCAGCAGA AATGAAGTGG ACCGCACCGC TGCTGATCAT 
CAGACCATTC TGCGAGCCTG GGCTGTGAAG GATTTTGCTC CAAACTGTCC TCTTTATGTC 
CAGATCCTCA AACCCGAGAA CAAATTTCAT GTCAAATTTG CAGATCACGT GGTGTGTGAA 
GAGGAGTTCA AGTACGCCAT GCTGGCCCTG AACTGTGTGT GTCCCGCCAC CTCCACACTG 
GTCACCTTGT TGGTGCACAC GTCTCGAGGA CAAGAAGGTC AGACGTCTCC AGAACAGTGG 
CAGAGGACTT ACGGTCGCTG CTCGGGGAAT GAAGTTTATC ACATCCGTTT GATGGACAGC 
AAGTTTTTTG GAGAATATGA TGGGAAGAGT TTCACTTATG CCTCCTTCCA CGCACACAAG 
AAGTATGGTG TGTGTCTAAT CGGCACAAAG AGAGAAGACA ACAAGAGCAT TCTCCTGAAC 
CCCGGCCCTC GCCACATCAT GTCCGCCACA GACACCTGCT ACTACATCAA CATCACCAAA 
GAGGAGAACT CGGCCTTCAT CTTCAAGCAG GAGGAGAAGC ACAGTAAAGG CTTATCAGCC 
GCCGGCGTGT ACGACGCTCC GTCTCGCCTC CCAGTGCAAA GCATCATTGC CAGCATGGGA 
ACTGTGGCCA TTGACCTGCA GAACCCGGAT CCACCGGCTG ACAGCAATAA ACTGGCTCTG 
CCCACGGAGA ACGGAGCGGG CAGCCGCAGA CCCAGCATTG CCCCTGTGCT GGAGATCGCA 
GACTCCTCTG CCATCCTGCC CTGTGATCTC CTCAACGACC CCTCAGAAGA CGAGACCAAC 
CAATCAGATG AGGATGGAGC GCCGGCCATA GACTGTGTTA AAGGCTATCC TCCAAACTCC 
CCTTACATTG GAAGCTCTCC AACACTTTGC CACCTTCTTC AACAGAAGGC ACCGTTTTGC 
TGTCTACGAC TTGACAAGGG TTGTCGACAC AATAGTTTCG AAGACGCCAA GGCGTATGGT 
TTCAAGAACA AGTTGATTAT CGTTTCTGCT GAGACAGCGG GAAATGGCTT GTACAACTTC 
ATAGTGCCGT TACGTGCCTA TTACCGACCG CGGAAGGAGC TGAATCCCAT TGTGCTGCTG 
CTGGATAATC CGCCTGATAA TCATTTCCTT GAGGCCATCT GCTGTTTCCC TATGGTGTAC 
TACATGGCGG GAACCATAGA CAATTTGGAT AACCTGCTGC AGTGTGGCAT CATCTACGCT 
GATAACCTGG TGGTGGTGGA TAAGGAGAGC ACTATGAGCG CAGAGGAAGA CTACATGGCA 
GACGCTAAAA CCATAGTCAA TGTGCAGACC ATGTTCAGGT TGTTTCCTAG TCTCAGCATA 
ATCACTGAGC TAACTCATCC GTCTAATATG AGGTTCATGC AGTTCCGTGC CAAAGACTGC 
TACTCCCTGG CTCTCTCCAA ACTCGAAAAG ATAGAGCGAG ATAAGGGATC GAATCTGGCG 
TTCATGTTCC GCCTGCCGTT CGCTGCAGGC AGAGTCTTCA GTATCAGCAT GCTGGATACT 
CTGCTTTACC AGTCGTTTGT GAAGGACTAT ATGATTCTCA TTGCGCGGCT CCTCCTGGGT 
TTAGACACCA CCCCGGGCTC AGGATACCTC TGTGCTATGA AGGTAACAGA AGAGGACTTA 
TGGATTCGAA CGTATGGGAG ACTCTTCCAG AAGCTGTGTT CCTCCAGCGC AGAGATTCCC 
ATCGGGATTT ACCGCACCGA ATCCCACATG TTCTCCTCTT CAGAGTCTCA GGTGTCTGTG 
GAGGATTGCG AGGACCAGCG GGACCACAGG GAATCCTGGA AAGAGAAAAC TGCCCATAGG 
AACTCCACAG GGAGCGACCA ATCAGAGCAT CCGCTCCTGC GCAAGAAAAG CATGCAGTGG 
GCGAGACGCC TAAGCAGAAA AAACACCAAA CCTCCCAGTC GAGCTGAACG CATCAATCAA 
CAGCGCCTGA ACCTGTACCG GCGCTCCGAG AGGCAGGAGC TGTCAGAGCT GGTGAAGAAC 
CGCATGAAGC ATTTAGGCCT GCCCACTGTG GGATACGAGG ACGTAGCTAA TTTAACCGCA 
AGCGATGTGA TGAACCGAGT AAATCTGGGA TATTTGCAAG AGGGTACTTA CATGACAGAG 
GGTACTGCAG TGGCTCAAAC AGATGAAATG AATGACAATC AGAACACGTT GTCATATGTG 
CTGATAAACC CTCCGCCGGA CACCATTCTG GAGCTCAATG ACATTGTCTA CATCATCCGT 
TCTGATCCAC TGGCACATGT CCCTGAAAAT CTCCCAGGGG CTCCAGCTGG AGCGAAGATA 
CGCCAAGACT ATCGGCCCGA GACAAGGGAT GAGACGCATC TGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:dkey-21e5.1 ( XM_021476676.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332351.1 Danio rerio si:dkey-21e5.1 (si:dkey-21e5.1), transcript variant X4, mRNA.

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CloneID	ODa142856
Clone ID Related Accession (Same CDS sequence)	XM_021476676.1
Accession Version	XM_021476676.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3744bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	potassium channel subfamily T member 1 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGGTG CCAAAAGAAC CGAGTCGCCG TCTGAAATCG ATTCGGACGT GAAGACAGAA 
GCAAGGAAAT CTTTCGAGCA CATTTGGCTC AATTCTGCGG TGCCCGTCCG GACCATGGGG 
TCTTTTGAGA GCGATGCTGG CCAAAGGTAC ACAGAGGAGT ACAGTATGGA TTCAACCAAT 
GCACAGGTGC AGGTGGAGTT TTACGTAAAC GAAAACACAT TTAAGGAGAG GTTGAAGCTC 
TTCTTCATCA AAAACCAAAG GTCAAGTCTC AGAATACGCC TCTTTAACCT CTCCTTAAAG 
TTGTTAACAT GCCTGCTCTA CATAATCAGA GTCTCACTTG ATGATCCAAC TAACCAGGTC 
ACATGTGCAC GGAAACACTG CGCAAACATC ACAGATGCTC TGTGCAGCAA AGCCTGTGCC 
AACTATACAG CAAACCTGAC CGCAGATCCC ACACAAATAA ACTGGGAGTT AATATTTTGG 
GTGAATAGAA GAGTTCCTGT ATGGGCGATA CAGGTGACAG TGGCCTTAAT TAGCTTCTTG 
CAAGCGATGC TCCTGGTGTA TCTCAGCTAC AAGGGCAACA TTTGGGAACA GATTTTCCAA 
ATCTCCTTCA TCATTGAGAT GATCAATACA GTGCCATTCA TAATTACAAT CTTTTGGCCT 
CCGCTTAGGA ACATATTTGT TCCTGTATTT CTTAACTGTT GGCTTGCCAA ATCAGCGCTG 
GAGAATATGA TTAATGATCT CCATCGTGCC ATCCAGCGCA CACACTCTGC CATGTTCAAC 
CAGGTCCTCA TCCTGATCTG TACACTGCTC TGTCTGGTCT TCACCGGGGC TTGTGGGATT 
CAGCACCTGG AGCGAGCAGG GAAGAATCTG ACACTCTTTG ACGCTTTATA TTTCTGCATC 
GTCACCTTCT CCACTGTTGG ATATGGAGAT GTGACGCCCA GAATCTGGCC CTCTCAGCTG 
CTGGTGGTCA TCATGATCTG TGTGGCGCTG GTGGTGCTGC CATTACAGTT CGAGGAGTTG 
GCGTATCTAT GGATGGAGAG GCAGAAGTCA GGTGGAAACT ACAGCAGACA CCGCGCTCAG 
ACAGAGAAAC ACGTGGTGCT CTGCGTCACC TCACTCAAAA TAGACCTGCT CATGGATTTC 
CTCAATGAGT TCTATGCCCA CCCGAGACTA CAGGACTATT ATGTGGTGAT CCTCTGTCCC 
ACGGAGATGG ACATCCAGGT GCGGCGAATA CTGCAGATCC CACTGTGGTC TCAGCGGGTC 
ATCTACCTCC AAGGCTCTGC ACTGAAGGAC CAAGACCTCA TGAGGGCCAA GATGGATGAT 
GCGGAGGCCT GCTTCATCCT CAGCAGCAGA AATGAAGTGG ACCGCACCGC TGCTGATCAT 
CAGACCATTC TGCGAGCCTG GGCTGTGAAG GATTTTGCTC CAAACTGTCC TCTTTATGTC 
CAGATCCTCA AACCCGAGAA CAAATTTCAT GTCAAATTTG CAGGTATGTT TTGTTTGCTA 
GATCACGTGG TGTGTGAAGA GGAGTTCAAG TACGCCATGC TGGCCCTGAA CTGTGTGTGT 
CCCGCCACCT CCACACTGGT CACCTTGTTG GTGCACACGT CTCGAGGACA AGAAGGTCAG 
ACGTCTCCAG AACAGTGGCA GAGGACTTAC GGTCGCTGCT CGGGGAATGA AGTTTATCAC 
ATCCGTTTGA TGGACAGCAA GTTTTTTGGA GAATATGATG GGAAGAGTTT CACTTATGCC 
TCCTTCCACG CACACAAGAA GTATGGTGTG TGTCTAATCG GCACAAAGAG AGAAGACAAC 
AAGAGCATTC TCCTGAACCC CGGCCCTCGC CACATCATGT CCGCCACAGA CACCTGCTAC 
TACATCAACA TCACCAAAGA GGAGAACTCG GCCTTCATCT TCAAGCAGGA GGAGAAGCAC 
AGTAAAGGCT TATCAGCCGC CGGCGTGTAC GACGCTCCGT CTCGCCTCCC AGTGCAAAGC 
ATCATTGCCA GCATGGTTGA TCAGTCTACT TCATATGGAA CTGTGGCCAT TGACCTGCAG 
AACCCGGATC CACCGGCTGA CAGCAATAAA CTGGCTCTGC CCACGGAGAA CGGAGCGGGC 
AGCCGCAGAC CCAGCATTGC CCCTGTGCTG GAGATCGCAG ACTCCTCTGC CATCCTGCCC 
TGTGATCTCC TCAACGACCC CTCAGAAGAC GAGACCAACC AATCAGATGA GGATGGAGCG 
CCGGCCATAG ACTGTGTTAA AGGCTATCCT CCAAACTCCC CTTACATTGG AAGCTCTCCA 
ACACTTTGCC ACCTTCTTCA ACAGAAGGCA CCGTTTTGCT GTCTACGACT TGACAAGGGT 
TGTCGACACA ATAGTTTCGA AGACGCCAAG GCGTATGGTT TCAAGAACAA GTTGATTATC 
GTTTCTGCTG AGACAGCGGG AAATGGCTTG TACAACTTCA TAGTGCCGTT ACGTGCCTAT 
TACCGACCGC GGAAGGAGCT GAATCCCATT GTGCTGCTGC TGGATAATCC GCCTGATAAT 
CATTTCCTTG AGGCCATCTG CTGTTTCCCT ATGGTGTACT ACATGGCGGG AACCATAGAC 
AATTTGGATA ACCTGCTGCA GTGTGGCATC ATCTACGCTG ATAACCTGGT GGTGGTGGAT 
AAGGAGAGCA CTATGAGCGC AGAGGAAGAC TACATGGCAG ACGCTAAAAC CATAGTCAAT 
GTGCAGACCA TGTTCAGGTT GTTTCCTAGT CTCAGCATAA TCACTGAGCT AACTCATCCG 
TCTAATATGA GGTTCATGCA GTTCCGTGCC AAAGACTGCT ACTCCCTGGC TCTCTCCAAA 
CTCGAAAAGA TAGAGCGAGA TAAGGGATCG AATCTGGCGT TCATGTTCCG CCTGCCGTTC 
GCTGCAGGCA GAGTCTTCAG TATCAGCATG CTGGATACTC TGCTTTACCA GTCGTTTGTG 
AAGGACTATA TGATTCTCAT TGCGCGGCTC CTCCTGGGTT TAGACACCAC CCCGGGCTCA 
GGATACCTCT GTGCTATGAA GGTAACAGAA GAGGACTTAT GGATTCGAAC GTATGGGAGA 
CTCTTCCAGA AGCTGTGTTC CTCCAGCGCA GAGATTCCCA TCGGGATTTA CCGCACCGAA 
TCCCACATGT TCTCCTCTTC AGAGTCTCAG GTGTCTGTGG AGGATTGCGA GGACCAGCGG 
GACCACAGGG AATCCTGGAA AGAGAAAACT GCCCATAGGA ACTCCACAGG GAGCGACCAA 
TCAGAGCATC CGCTCCTGCG CAAGAAAAGC ATGCAGTGGG CGAGACGCCT AAGCAGAAAA 
AACACCAAAC CTCCCAGTCG AGCTGAACGC ATCAATCAAC AGCGCCTGAA CCTGTACCGG 
CGCTCCGAGA GGCAGGAGCT GTCAGAGCTG GTGAAGAACC GCATGAAGCA TTTAGGCCTG 
CCCACTGTGG GATACGAGGA CGTAGCTAAT TTAACCGCAA GCGATGTGAT GAACCGAGTA 
AATCTGGGAT ATTTGCAAGA GGGTACTTAC ATGACAGAGG GTACTGCAGT GGCTCAAACA 
GATGAAATGA ATGACAATCA GAACACGTTG TCATATGTGC TGATAAACCC TCCGCCGGAC 
ACCATTCTGG AGCTCAATGA CATTGTCTAC ATCATCCGTT CTGATCCACT GGCACATGTC 
CCTGAAAATC TCCCAGGGGC TCCAGCTGGA GCGAAGATAC GCCAAGACTA TCGGCCCGAG 
ACAAGGGATG AGACGCATCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332351.1
CDS	481..4224
Translation	MAGAKRTESPSEIDSDVKTEARKSFEHIWLNSAVPVRTMGSFESDAGQRYTEEYSMDSTNAQVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNLSLKLLTCLLYIIRVSLDDPTNQVTCARKHCANITDALCSKACANYTANLTADPTQINWELIFWVNRRVPVWAIQVTVALISFLQAMLLVYLSYKGNIWEQIFQISFIIEMINTVPFIITIFWPPLRNIFVPVFLNCWLAKSALENMINDLHRAIQRTHSAMFNQVLILICTLLCLVFTGACGIQHLERAGKNLTLFDALYFCIVTFSTVGYGDVTPRIWPSQLLVVIMICVALVVLPLQFEELAYLWMERQKSGGNYSRHRAQTEKHVVLCVTSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRILQIPLWSQRVIYLQGSALKDQDLMRAKMDDAEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAGMFCLLDHVVCEEEFKYAMLALNCVCPATSTLVTLLVHTSRGQEGQTSPEQWQRTYGRCSGNEVYHIRLMDSKFFGEYDGKSFTYASFHAHKKYGVCLIGTKREDNKSILLNPGPRHIMSATDTCYYINITKEENSAFIFKQEEKHSKGLSAAGVYDAPSRLPVQSIIASMVDQSTSYGTVAIDLQNPDPPADSNKLALPTENGAGSRRPSIAPVLEIADSSAILPCDLLNDPSEDETNQSDEDGAPAIDCVKGYPPNSPYIGSSPTLCHLLQQKAPFCCLRLDKGCRHNSFEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRKELNPIVLLLDNPPDNHFLEAICCFPMVYYMAGTIDNLDNLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSIITELTHPSNMRFMQFRAKDCYSLALSKLEKIERDKGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMILIARLLLGLDTTPGSGYLCAMKVTEEDLWIRTYGRLFQKLCSSSAEIPIGIYRTESHMFSSSESQVSVEDCEDQRDHRESWKEKTAHRNSTGSDQSEHPLLRKKSMQWARRLSRKNTKPPSRAERINQQRLNLYRRSERQELSELVKNRMKHLGLPTVGYEDVANLTASDVMNRVNLGYLQEGTYMTEGTAVAQTDEMNDNQNTLSYVLINPPPDTILELNDIVYIIRSDPLAHVPENLPGAPAGAKIRQDYRPETRDETHL

Target ORF information:

RefSeq Version	XM_021476676.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:dkey-21e5.1 (si:dkey-21e5.1), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476676.1

ORF Insert Sequence:

ATGGCAGGTG CCAAAAGAAC CGAGTCGCCG TCTGAAATCG ATTCGGACGT GAAGACAGAA 
GCAAGGAAAT CTTTCGAGCA CATTTGGCTC AATTCTGCGG TGCCCGTCCG GACCATGGGG 
TCTTTTGAGA GCGATGCTGG CCAAAGGTAC ACAGAGGAGT ACAGTATGGA TTCAACCAAT 
GCACAGGTGC AGGTGGAGTT TTACGTAAAC GAAAACACAT TTAAGGAGAG GTTGAAGCTC 
TTCTTCATCA AAAACCAAAG GTCAAGTCTC AGAATACGCC TCTTTAACCT CTCCTTAAAG 
TTGTTAACAT GCCTGCTCTA CATAATCAGA GTCTCACTTG ATGATCCAAC TAACCAGGTC 
ACATGTGCAC GGAAACACTG CGCAAACATC ACAGATGCTC TGTGCAGCAA AGCCTGTGCC 
AACTATACAG CAAACCTGAC CGCAGATCCC ACACAAATAA ACTGGGAGTT AATATTTTGG 
GTGAATAGAA GAGTTCCTGT ATGGGCGATA CAGGTGACAG TGGCCTTAAT TAGCTTCTTG 
CAAGCGATGC TCCTGGTGTA TCTCAGCTAC AAGGGCAACA TTTGGGAACA GATTTTCCAA 
ATCTCCTTCA TCATTGAGAT GATCAATACA GTGCCATTCA TAATTACAAT CTTTTGGCCT 
CCGCTTAGGA ACATATTTGT TCCTGTATTT CTTAACTGTT GGCTTGCCAA ATCAGCGCTG 
GAGAATATGA TTAATGATCT CCATCGTGCC ATCCAGCGCA CACACTCTGC CATGTTCAAC 
CAGGTCCTCA TCCTGATCTG TACACTGCTC TGTCTGGTCT TCACCGGGGC TTGTGGGATT 
CAGCACCTGG AGCGAGCAGG GAAGAATCTG ACACTCTTTG ACGCTTTATA TTTCTGCATC 
GTCACCTTCT CCACTGTTGG ATATGGAGAT GTGACGCCCA GAATCTGGCC CTCTCAGCTG 
CTGGTGGTCA TCATGATCTG TGTGGCGCTG GTGGTGCTGC CATTACAGTT CGAGGAGTTG 
GCGTATCTAT GGATGGAGAG GCAGAAGTCA GGTGGAAACT ACAGCAGACA CCGCGCTCAG 
ACAGAGAAAC ACGTGGTGCT CTGCGTCACC TCACTCAAAA TAGACCTGCT CATGGATTTC 
CTCAATGAGT TCTATGCCCA CCCGAGACTA CAGGACTATT ATGTGGTGAT CCTCTGTCCC 
ACGGAGATGG ACATCCAGGT GCGGCGAATA CTGCAGATCC CACTGTGGTC TCAGCGGGTC 
ATCTACCTCC AAGGCTCTGC ACTGAAGGAC CAAGACCTCA TGAGGGCCAA GATGGATGAT 
GCGGAGGCCT GCTTCATCCT CAGCAGCAGA AATGAAGTGG ACCGCACCGC TGCTGATCAT 
CAGACCATTC TGCGAGCCTG GGCTGTGAAG GATTTTGCTC CAAACTGTCC TCTTTATGTC 
CAGATCCTCA AACCCGAGAA CAAATTTCAT GTCAAATTTG CAGGTATGTT TTGTTTGCTA 
GATCACGTGG TGTGTGAAGA GGAGTTCAAG TACGCCATGC TGGCCCTGAA CTGTGTGTGT 
CCCGCCACCT CCACACTGGT CACCTTGTTG GTGCACACGT CTCGAGGACA AGAAGGTCAG 
ACGTCTCCAG AACAGTGGCA GAGGACTTAC GGTCGCTGCT CGGGGAATGA AGTTTATCAC 
ATCCGTTTGA TGGACAGCAA GTTTTTTGGA GAATATGATG GGAAGAGTTT CACTTATGCC 
TCCTTCCACG CACACAAGAA GTATGGTGTG TGTCTAATCG GCACAAAGAG AGAAGACAAC 
AAGAGCATTC TCCTGAACCC CGGCCCTCGC CACATCATGT CCGCCACAGA CACCTGCTAC 
TACATCAACA TCACCAAAGA GGAGAACTCG GCCTTCATCT TCAAGCAGGA GGAGAAGCAC 
AGTAAAGGCT TATCAGCCGC CGGCGTGTAC GACGCTCCGT CTCGCCTCCC AGTGCAAAGC 
ATCATTGCCA GCATGGTTGA TCAGTCTACT TCATATGGAA CTGTGGCCAT TGACCTGCAG 
AACCCGGATC CACCGGCTGA CAGCAATAAA CTGGCTCTGC CCACGGAGAA CGGAGCGGGC 
AGCCGCAGAC CCAGCATTGC CCCTGTGCTG GAGATCGCAG ACTCCTCTGC CATCCTGCCC 
TGTGATCTCC TCAACGACCC CTCAGAAGAC GAGACCAACC AATCAGATGA GGATGGAGCG 
CCGGCCATAG ACTGTGTTAA AGGCTATCCT CCAAACTCCC CTTACATTGG AAGCTCTCCA 
ACACTTTGCC ACCTTCTTCA ACAGAAGGCA CCGTTTTGCT GTCTACGACT TGACAAGGGT 
TGTCGACACA ATAGTTTCGA AGACGCCAAG GCGTATGGTT TCAAGAACAA GTTGATTATC 
GTTTCTGCTG AGACAGCGGG AAATGGCTTG TACAACTTCA TAGTGCCGTT ACGTGCCTAT 
TACCGACCGC GGAAGGAGCT GAATCCCATT GTGCTGCTGC TGGATAATCC GCCTGATAAT 
CATTTCCTTG AGGCCATCTG CTGTTTCCCT ATGGTGTACT ACATGGCGGG AACCATAGAC 
AATTTGGATA ACCTGCTGCA GTGTGGCATC ATCTACGCTG ATAACCTGGT GGTGGTGGAT 
AAGGAGAGCA CTATGAGCGC AGAGGAAGAC TACATGGCAG ACGCTAAAAC CATAGTCAAT 
GTGCAGACCA TGTTCAGGTT GTTTCCTAGT CTCAGCATAA TCACTGAGCT AACTCATCCG 
TCTAATATGA GGTTCATGCA GTTCCGTGCC AAAGACTGCT ACTCCCTGGC TCTCTCCAAA 
CTCGAAAAGA TAGAGCGAGA TAAGGGATCG AATCTGGCGT TCATGTTCCG CCTGCCGTTC 
GCTGCAGGCA GAGTCTTCAG TATCAGCATG CTGGATACTC TGCTTTACCA GTCGTTTGTG 
AAGGACTATA TGATTCTCAT TGCGCGGCTC CTCCTGGGTT TAGACACCAC CCCGGGCTCA 
GGATACCTCT GTGCTATGAA GGTAACAGAA GAGGACTTAT GGATTCGAAC GTATGGGAGA 
CTCTTCCAGA AGCTGTGTTC CTCCAGCGCA GAGATTCCCA TCGGGATTTA CCGCACCGAA 
TCCCACATGT TCTCCTCTTC AGAGTCTCAG GTGTCTGTGG AGGATTGCGA GGACCAGCGG 
GACCACAGGG AATCCTGGAA AGAGAAAACT GCCCATAGGA ACTCCACAGG GAGCGACCAA 
TCAGAGCATC CGCTCCTGCG CAAGAAAAGC ATGCAGTGGG CGAGACGCCT AAGCAGAAAA 
AACACCAAAC CTCCCAGTCG AGCTGAACGC ATCAATCAAC AGCGCCTGAA CCTGTACCGG 
CGCTCCGAGA GGCAGGAGCT GTCAGAGCTG GTGAAGAACC GCATGAAGCA TTTAGGCCTG 
CCCACTGTGG GATACGAGGA CGTAGCTAAT TTAACCGCAA GCGATGTGAT GAACCGAGTA 
AATCTGGGAT ATTTGCAAGA GGGTACTTAC ATGACAGAGG GTACTGCAGT GGCTCAAACA 
GATGAAATGA ATGACAATCA GAACACGTTG TCATATGTGC TGATAAACCC TCCGCCGGAC 
ACCATTCTGG AGCTCAATGA CATTGTCTAC ATCATCCGTT CTGATCCACT GGCACATGTC 
CCTGAAAATC TCCCAGGGGC TCCAGCTGGA GCGAAGATAC GCCAAGACTA TCGGCCCGAG 
ACAAGGGATG AGACGCATCT GTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:dkey-21e5.1 ( XM_021476677.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332352.1 Danio rerio si:dkey-21e5.1 (si:dkey-21e5.1), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa142857
Clone ID Related Accession (Same CDS sequence)	XM_021476677.1
Accession Version	XM_021476677.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3660bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	potassium channel subfamily T member 1 isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGAC CGCGCAGTCA GTCTCAGGCA GAGCCAAGCA TGACTCCGCC ACGACGCTCT 
TCCACTGCTG CTGACAGGTT GCCTACGGAG ACCGTTCAGA GAAATAACAG CAGTAATACT 
GGTGTTATTC TGGATATTTC CACGCTGAAA TTGGCAGATG TGGACTCTGA GGTCCCTCCT 
CTACCTCCAC GATACCGCTT TCGAGATCTG CTGCTTGGGG ATCAGTCATT TCAGAACGAT 
GACAGGGTGC AGGTGGAGTT TTACGTAAAC GAAAACACAT TTAAGGAGAG GTTGAAGCTC 
TTCTTCATCA AAAACCAAAG GTCAAGTCTC AGAATACGCC TCTTTAACCT CTCCTTAAAG 
TTGTTAACAT GCCTGCTCTA CATAATCAGA GTCTCACTTG ATGATCCAAC TAACCAGGTC 
ACATGTGCAC GGAAACACTG CGCAAACATC ACAGATGCTC TGTGCAGCAA AGCCTGTGCC 
AACTATACAG CAAACCTGAC CGCAGATCCC ACACAAATAA ACTGGGAGTT AATATTTTGG 
GTGAATAGAA GAGTTCCTGT ATGGGCGATA CAGGTGACAG TGGCCTTAAT TAGCTTCTTG 
CAAGCGATGC TCCTGGTGTA TCTCAGCTAC AAGGGCAACA TTTGGGAACA GATTTTCCAA 
ATCTCCTTCA TCATTGAGAT GATCAATACA GTGCCATTCA TAATTACAAT CTTTTGGCCT 
CCGCTTAGGA ACATATTTGT TCCTGTATTT CTTAACTGTT GGCTTGCCAA ATCAGCGCTG 
GAGAATATGA TTAATGATCT CCATCGTGCC ATCCAGCGCA CACACTCTGC CATGTTCAAC 
CAGGTCCTCA TCCTGATCTG TACACTGCTC TGTCTGGTCT TCACCGGGGC TTGTGGGATT 
CAGCACCTGG AGCGAGCAGG GAAGAATCTG ACACTCTTTG ACGCTTTATA TTTCTGCATC 
GTCACCTTCT CCACTGTTGG ATATGGAGAT GTGACGCCCA GAATCTGGCC CTCTCAGCTG 
CTGGTGGTCA TCATGATCTG TGTGGCGCTG GTGGTGCTGC CATTACAGTT CGAGGAGTTG 
GCGTATCTAT GGATGGAGAG GCAGAAGTCA GGTGGAAACT ACAGCAGACA CCGCGCTCAG 
ACAGAGAAAC ACGTGGTGCT CTGCGTCACC TCACTCAAAA TAGACCTGCT CATGGATTTC 
CTCAATGAGT TCTATGCCCA CCCGAGACTA CAGGACTATT ATGTGGTGAT CCTCTGTCCC 
ACGGAGATGG ACATCCAGGT GCGGCGAATA CTGCAGATCC CACTGTGGTC TCAGCGGGTC 
ATCTACCTCC AAGGCTCTGC ACTGAAGGAC CAAGACCTCA TGAGGGCCAA GATGGATGAT 
GCGGAGGCCT GCTTCATCCT CAGCAGCAGA AATGAAGTGG ACCGCACCGC TGCTGATCAT 
CAGACCATTC TGCGAGCCTG GGCTGTGAAG GATTTTGCTC CAAACTGTCC TCTTTATGTC 
CAGATCCTCA AACCCGAGAA CAAATTTCAT GTCAAATTTG CAGATCACGT GGTGTGTGAA 
GAGGAGTTCA AGTACGCCAT GCTGGCCCTG AACTGTGTGT GTCCCGCCAC CTCCACACTG 
GTCACCTTGT TGGTGCACAC GTCTCGAGGA CAAGAAGGTC AGACGTCTCC AGAACAGTGG 
CAGAGGACTT ACGGTCGCTG CTCGGGGAAT GAAGTTTATC ACATCCGTTT GATGGACAGC 
AAGTTTTTTG GAGAATATGA TGGGAAGAGT TTCACTTATG CCTCCTTCCA CGCACACAAG 
AAGTATGGTG TGTGTCTAAT CGGCACAAAG AGAGAAGACA ACAAGAGCAT TCTCCTGAAC 
CCCGGCCCTC GCCACATCAT GTCCGCCACA GACACCTGCT ACTACATCAA CATCACCAAA 
GAGGAGAACT CGGCCTTCAT CTTCAAGCAG GAGGAGAAGC ACAGTAAAGG CTTATCAGCC 
GCCGGCGTGT ACGACGCTCC GTCTCGCCTC CCAGTGCAAA GCATCATTGC CAGCATGGGA 
ACTGTGGCCA TTGACCTGCA GAACCCGGAT CCACCGGCTG ACAGCAATAA ACTGGCTCTG 
CCCACGGAGA ACGGAGCGGG CAGCCGCAGA CCCAGCATTG CCCCTGTGCT GGAGATCGCA 
GACTCCTCTG CCATCCTGCC CTGTGATCTC CTCAACGACC CCTCAGAAGA CGAGACCAAC 
CAATCAGATG AGGATGGAGC GCCGGCCATA GACTGTGTTA AAGGCTATCC TCCAAACTCC 
CCTTACATTG GAAGCTCTCC AACACTTTGC CACCTTCTTC AACAGAAGGC ACCGTTTTGC 
TGTCTACGAC TTGACAAGGG TTGTCGACAC AATAGTTTCG AAGACGCCAA GGCGTATGGT 
TTCAAGAACA AGTTGATTAT CGTTTCTGCT GAGACAGCGG GAAATGGCTT GTACAACTTC 
ATAGTGCCGT TACGTGCCTA TTACCGACCG CGGAAGGAGC TGAATCCCAT TGTGCTGCTG 
CTGGATAATC CGCCTGATAA TCATTTCCTT GAGGCCATCT GCTGTTTCCC TATGGTGTAC 
TACATGGCGG GAACCATAGA CAATTTGGAT AACCTGCTGC AGTGTGGCAT CATCTACGCT 
GATAACCTGG TGGTGGTGGA TAAGGAGAGC ACTATGAGCG CAGAGGAAGA CTACATGGCA 
GACGCTAAAA CCATAGTCAA TGTGCAGACC ATGTTCAGGT TGTTTCCTAG TCTCAGCATA 
ATCACTGAGC TAACTCATCC GTCTAATATG AGGTTCATGC AGTTCCGTGC CAAAGACTGC 
TACTCCCTGG CTCTCTCCAA ACTCGAAAAG ATAGAGCGAG ATAAGGGATC GAATCTGGCG 
TTCATGTTCC GCCTGCCGTT CGCTGCAGGC AGAGTCTTCA GTATCAGCAT GCTGGATACT 
CTGCTTTACC AGTCGTTTGT GAAGGACTAT ATGATTCTCA TTGCGCGGCT CCTCCTGGGT 
TTAGACACCA CCCCGGGCTC AGGATACCTC TGTGCTATGA AGGTAACAGA AGAGGACTTA 
TGGATTCGAA CGTATGGGAG ACTCTTCCAG AAGCTGTGTT CCTCCAGCGC AGAGATTCCC 
ATCGGGATTT ACCGCACCGA ATCCCACATG TTCTCCTCTT CAGAGTCTCA GGTGTCTGTG 
GAGGATTGCG AGGACCAGCG GGACCACAGG GAATCCTGGA AAGAGAAAAC TGCCCATAGG 
AACTCCACAG GGAGCGACCA ATCAGAGCAT CCGCTCCTGC GCAAGAAAAG CATGCAGTGG 
GCGAGACGCC TAAGCAGAAA AAACACCAAA CCTCCCAGTC GAGCTGAACG CATCAATCAA 
CAGCGCCTGA ACCTGTACCG GCGCTCCGAG AGGCAGGAGC TGTCAGAGCT GGTGAAGAAC 
CGCATGAAGC ATTTAGGCCT GCCCACTGTG GGATACGATG AAATGAATGA CAATCAGAAC 
ACGTTGTCAT ATGTGCTGAT AAACCCTCCG CCGGACACCA TTCTGGAGCT CAATGACATT 
GTCTACATCA TCCGTTCTGA TCCACTGGCA CATGTCCCTG AAAATCTCCC AGGGGCTCCA 
GCTGGAGCGA AGATACGCCA AGACTATCGG CCCGAGACAA GGGATGAGAC GCATCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332352.1
CDS	703..4362
Translation	MMGPRSQSQAEPSMTPPRRSSTAADRLPTETVQRNNSSNTGVILDISTLKLADVDSEVPPLPPRYRFRDLLLGDQSFQNDDRVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNLSLKLLTCLLYIIRVSLDDPTNQVTCARKHCANITDALCSKACANYTANLTADPTQINWELIFWVNRRVPVWAIQVTVALISFLQAMLLVYLSYKGNIWEQIFQISFIIEMINTVPFIITIFWPPLRNIFVPVFLNCWLAKSALENMINDLHRAIQRTHSAMFNQVLILICTLLCLVFTGACGIQHLERAGKNLTLFDALYFCIVTFSTVGYGDVTPRIWPSQLLVVIMICVALVVLPLQFEELAYLWMERQKSGGNYSRHRAQTEKHVVLCVTSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRILQIPLWSQRVIYLQGSALKDQDLMRAKMDDAEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEEFKYAMLALNCVCPATSTLVTLLVHTSRGQEGQTSPEQWQRTYGRCSGNEVYHIRLMDSKFFGEYDGKSFTYASFHAHKKYGVCLIGTKREDNKSILLNPGPRHIMSATDTCYYINITKEENSAFIFKQEEKHSKGLSAAGVYDAPSRLPVQSIIASMGTVAIDLQNPDPPADSNKLALPTENGAGSRRPSIAPVLEIADSSAILPCDLLNDPSEDETNQSDEDGAPAIDCVKGYPPNSPYIGSSPTLCHLLQQKAPFCCLRLDKGCRHNSFEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRKELNPIVLLLDNPPDNHFLEAICCFPMVYYMAGTIDNLDNLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSIITELTHPSNMRFMQFRAKDCYSLALSKLEKIERDKGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMILIARLLLGLDTTPGSGYLCAMKVTEEDLWIRTYGRLFQKLCSSSAEIPIGIYRTESHMFSSSESQVSVEDCEDQRDHRESWKEKTAHRNSTGSDQSEHPLLRKKSMQWARRLSRKNTKPPSRAERINQQRLNLYRRSERQELSELVKNRMKHLGLPTVGYDEMNDNQNTLSYVLINPPPDTILELNDIVYIIRSDPLAHVPENLPGAPAGAKIRQDYRPETRDETHL

Target ORF information:

RefSeq Version	XM_021476677.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:dkey-21e5.1 (si:dkey-21e5.1), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476677.1

ORF Insert Sequence:

ATGATGGGAC CGCGCAGTCA GTCTCAGGCA GAGCCAAGCA TGACTCCGCC ACGACGCTCT 
TCCACTGCTG CTGACAGGTT GCCTACGGAG ACCGTTCAGA GAAATAACAG CAGTAATACT 
GGTGTTATTC TGGATATTTC CACGCTGAAA TTGGCAGATG TGGACTCTGA GGTCCCTCCT 
CTACCTCCAC GATACCGCTT TCGAGATCTG CTGCTTGGGG ATCAGTCATT TCAGAACGAT 
GACAGGGTGC AGGTGGAGTT TTACGTAAAC GAAAACACAT TTAAGGAGAG GTTGAAGCTC 
TTCTTCATCA AAAACCAAAG GTCAAGTCTC AGAATACGCC TCTTTAACCT CTCCTTAAAG 
TTGTTAACAT GCCTGCTCTA CATAATCAGA GTCTCACTTG ATGATCCAAC TAACCAGGTC 
ACATGTGCAC GGAAACACTG CGCAAACATC ACAGATGCTC TGTGCAGCAA AGCCTGTGCC 
AACTATACAG CAAACCTGAC CGCAGATCCC ACACAAATAA ACTGGGAGTT AATATTTTGG 
GTGAATAGAA GAGTTCCTGT ATGGGCGATA CAGGTGACAG TGGCCTTAAT TAGCTTCTTG 
CAAGCGATGC TCCTGGTGTA TCTCAGCTAC AAGGGCAACA TTTGGGAACA GATTTTCCAA 
ATCTCCTTCA TCATTGAGAT GATCAATACA GTGCCATTCA TAATTACAAT CTTTTGGCCT 
CCGCTTAGGA ACATATTTGT TCCTGTATTT CTTAACTGTT GGCTTGCCAA ATCAGCGCTG 
GAGAATATGA TTAATGATCT CCATCGTGCC ATCCAGCGCA CACACTCTGC CATGTTCAAC 
CAGGTCCTCA TCCTGATCTG TACACTGCTC TGTCTGGTCT TCACCGGGGC TTGTGGGATT 
CAGCACCTGG AGCGAGCAGG GAAGAATCTG ACACTCTTTG ACGCTTTATA TTTCTGCATC 
GTCACCTTCT CCACTGTTGG ATATGGAGAT GTGACGCCCA GAATCTGGCC CTCTCAGCTG 
CTGGTGGTCA TCATGATCTG TGTGGCGCTG GTGGTGCTGC CATTACAGTT CGAGGAGTTG 
GCGTATCTAT GGATGGAGAG GCAGAAGTCA GGTGGAAACT ACAGCAGACA CCGCGCTCAG 
ACAGAGAAAC ACGTGGTGCT CTGCGTCACC TCACTCAAAA TAGACCTGCT CATGGATTTC 
CTCAATGAGT TCTATGCCCA CCCGAGACTA CAGGACTATT ATGTGGTGAT CCTCTGTCCC 
ACGGAGATGG ACATCCAGGT GCGGCGAATA CTGCAGATCC CACTGTGGTC TCAGCGGGTC 
ATCTACCTCC AAGGCTCTGC ACTGAAGGAC CAAGACCTCA TGAGGGCCAA GATGGATGAT 
GCGGAGGCCT GCTTCATCCT CAGCAGCAGA AATGAAGTGG ACCGCACCGC TGCTGATCAT 
CAGACCATTC TGCGAGCCTG GGCTGTGAAG GATTTTGCTC CAAACTGTCC TCTTTATGTC 
CAGATCCTCA AACCCGAGAA CAAATTTCAT GTCAAATTTG CAGATCACGT GGTGTGTGAA 
GAGGAGTTCA AGTACGCCAT GCTGGCCCTG AACTGTGTGT GTCCCGCCAC CTCCACACTG 
GTCACCTTGT TGGTGCACAC GTCTCGAGGA CAAGAAGGTC AGACGTCTCC AGAACAGTGG 
CAGAGGACTT ACGGTCGCTG CTCGGGGAAT GAAGTTTATC ACATCCGTTT GATGGACAGC 
AAGTTTTTTG GAGAATATGA TGGGAAGAGT TTCACTTATG CCTCCTTCCA CGCACACAAG 
AAGTATGGTG TGTGTCTAAT CGGCACAAAG AGAGAAGACA ACAAGAGCAT TCTCCTGAAC 
CCCGGCCCTC GCCACATCAT GTCCGCCACA GACACCTGCT ACTACATCAA CATCACCAAA 
GAGGAGAACT CGGCCTTCAT CTTCAAGCAG GAGGAGAAGC ACAGTAAAGG CTTATCAGCC 
GCCGGCGTGT ACGACGCTCC GTCTCGCCTC CCAGTGCAAA GCATCATTGC CAGCATGGGA 
ACTGTGGCCA TTGACCTGCA GAACCCGGAT CCACCGGCTG ACAGCAATAA ACTGGCTCTG 
CCCACGGAGA ACGGAGCGGG CAGCCGCAGA CCCAGCATTG CCCCTGTGCT GGAGATCGCA 
GACTCCTCTG CCATCCTGCC CTGTGATCTC CTCAACGACC CCTCAGAAGA CGAGACCAAC 
CAATCAGATG AGGATGGAGC GCCGGCCATA GACTGTGTTA AAGGCTATCC TCCAAACTCC 
CCTTACATTG GAAGCTCTCC AACACTTTGC CACCTTCTTC AACAGAAGGC ACCGTTTTGC 
TGTCTACGAC TTGACAAGGG TTGTCGACAC AATAGTTTCG AAGACGCCAA GGCGTATGGT 
TTCAAGAACA AGTTGATTAT CGTTTCTGCT GAGACAGCGG GAAATGGCTT GTACAACTTC 
ATAGTGCCGT TACGTGCCTA TTACCGACCG CGGAAGGAGC TGAATCCCAT TGTGCTGCTG 
CTGGATAATC CGCCTGATAA TCATTTCCTT GAGGCCATCT GCTGTTTCCC TATGGTGTAC 
TACATGGCGG GAACCATAGA CAATTTGGAT AACCTGCTGC AGTGTGGCAT CATCTACGCT 
GATAACCTGG TGGTGGTGGA TAAGGAGAGC ACTATGAGCG CAGAGGAAGA CTACATGGCA 
GACGCTAAAA CCATAGTCAA TGTGCAGACC ATGTTCAGGT TGTTTCCTAG TCTCAGCATA 
ATCACTGAGC TAACTCATCC GTCTAATATG AGGTTCATGC AGTTCCGTGC CAAAGACTGC 
TACTCCCTGG CTCTCTCCAA ACTCGAAAAG ATAGAGCGAG ATAAGGGATC GAATCTGGCG 
TTCATGTTCC GCCTGCCGTT CGCTGCAGGC AGAGTCTTCA GTATCAGCAT GCTGGATACT 
CTGCTTTACC AGTCGTTTGT GAAGGACTAT ATGATTCTCA TTGCGCGGCT CCTCCTGGGT 
TTAGACACCA CCCCGGGCTC AGGATACCTC TGTGCTATGA AGGTAACAGA AGAGGACTTA 
TGGATTCGAA CGTATGGGAG ACTCTTCCAG AAGCTGTGTT CCTCCAGCGC AGAGATTCCC 
ATCGGGATTT ACCGCACCGA ATCCCACATG TTCTCCTCTT CAGAGTCTCA GGTGTCTGTG 
GAGGATTGCG AGGACCAGCG GGACCACAGG GAATCCTGGA AAGAGAAAAC TGCCCATAGG 
AACTCCACAG GGAGCGACCA ATCAGAGCAT CCGCTCCTGC GCAAGAAAAG CATGCAGTGG 
GCGAGACGCC TAAGCAGAAA AAACACCAAA CCTCCCAGTC GAGCTGAACG CATCAATCAA 
CAGCGCCTGA ACCTGTACCG GCGCTCCGAG AGGCAGGAGC TGTCAGAGCT GGTGAAGAAC 
CGCATGAAGC ATTTAGGCCT GCCCACTGTG GGATACGATG AAATGAATGA CAATCAGAAC 
ACGTTGTCAT ATGTGCTGAT AAACCCTCCG CCGGACACCA TTCTGGAGCT CAATGACATT 
GTCTACATCA TCCGTTCTGA TCCACTGGCA CATGTCCCTG AAAATCTCCC AGGGGCTCCA 
GCTGGAGCGA AGATACGCCA AGACTATCGG CCCGAGACAA GGGATGAGAC GCATCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:dkey-21e5.1 ( XM_021476678.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332353.1 Danio rerio si:dkey-21e5.1 (si:dkey-21e5.1), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa142858
Clone ID Related Accession (Same CDS sequence)	XM_021476678.1
Accession Version	XM_021476678.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3723bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	potassium channel subfamily T member 1 isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGAC CGCGCAGTCA GTCTCAGGCA GAGCCAAGCA TGACTCCGCC ACGACGCTCT 
TCCACTGCTG CTGACAGGTT GCCTACGGAG ACCGTTCAGA GAAATAACAG CAGTAATACT 
GGTGTTATTC TGGATATTTC CACGCTGAAA TTGGCAGATG TGGACTCTGA GGTCCCTCCT 
CTACCTCCAC GATACCGCTT TCGAGATCTG CTGCTTGGGG ATCAGTCATT TCAGAACGAT 
GACAGGGTGC AGGTGGAGTT TTACGTAAAC GAAAACACAT TTAAGGAGAG GTTGAAGCTC 
TTCTTCATCA AAAACCAAAG GTCAAGTCTC AGAATACGCC TCTTTAACCT CTCCTTAAAG 
TTGTTAACAT GCCTGCTCTA CATAATCAGA GTCTCACTTG ATGATCCAAC TAACCAGGTC 
ACATGTGCAC GGAAACACTG CGCAAACATC ACAGATGCTC TGTGCAGCAA AGCCTGTGCC 
AACTATACAG CAAACCTGAC CGCAGATCCC ACACAAATAA ACTGGGAGTT AATATTTTGG 
GTGAATAGAA GAGTTCCTGT ATGGGCGATA CAGGTGACAG TGGCCTTAAT TAGCTTCTTG 
CAAGCGATGC TCCTGGTGTA TCTCAGCTAC AAGGGCAACA TTTGGGAACA GATTTTCCAA 
ATCTCCTTCA TCATTGAGAT GATCAATACA GTGCCATTCA TAATTACAAT CTTTTGGCCT 
CCGCTTAGGA ACATATTTGT TCCTGTATTT CTTAACTGTT GGCTTGCCAA ATCAGCGCTG 
GAGAATATGA TTAATGATCT CCATCGTGCC ATCCAGCGCA CACACTCTGC CATGTTCAAC 
CAGGTCCTCA TCCTGATCTG TACACTGCTC TGTCTGGTCT TCACCGGGGC TTGTGGGATT 
CAGCACCTGG AGCGAGCAGG GAAGAATCTG ACACTCTTTG ACGCTTTATA TTTCTGCATC 
GTCACCTTCT CCACTGTTGG ATATGGAGAT GTGACGCCCA GAATCTGGCC CTCTCAGCTG 
CTGGTGGTCA TCATGATCTG TGTGGCGCTG GTGGTGCTGC CATTACAGTT CGAGGAGTTG 
GCGTATCTAT GGATGGAGAG GCAGAAGTCA GGTGGAAACT ACAGCAGACA CCGCGCTCAG 
ACAGAGAAAC ACGTGGTGCT CTGCGTCACC TCACTCAAAA TAGACCTGCT CATGGATTTC 
CTCAATGAGT TCTATGCCCA CCCGAGACTA CAGGACTATT ATGTGGTGAT CCTCTGTCCC 
ACGGAGATGG ACATCCAGGT GCGGCGAATA CTGCAGATCC CACTGTGGTC TCAGCGGGTC 
ATCTACCTCC AAGGCTCTGC ACTGAAGGAC CAAGACCTCA TGAGGGCCAA GATGGATGAT 
GCGGAGGCCT GCTTCATCCT CAGCAGCAGA AATGAAGTGG ACCGCACCGC TGCTGATCAT 
CAGACCATTC TGCGAGCCTG GGCTGTGAAG GATTTTGCTC CAAACTGTCC TCTTTATGTC 
CAGATCCTCA AACCCGAGAA CAAATTTCAT GTCAAATTTG CAGATCACGT GGTGTGTGAA 
GAGGAGTTCA AGTACGCCAT GCTGGCCCTG AACTGTGTGT GTCCCGCCAC CTCCACACTG 
GTCACCTTGT TGGTGCACAC GTCTCGAGGA CAAGAAGGTC AGACGTCTCC AGAACAGTGG 
CAGAGGACTT ACGGTCGCTG CTCGGGGAAT GAAGTTTATC ACATCCGTTT GATGGACAGC 
AAGTTTTTTG GAGAATATGA TGGGAAGAGT TTCACTTATG CCTCCTTCCA CGCACACAAG 
AAGTATGGTG TGTGTCTAAT CGGCACAAAG AGAGAAGACA ACAAGAGCAT TCTCCTGAAC 
CCCGGCCCTC GCCACATCAT GTCCGCCACA GACACCTGCT ACTACATCAA CATCACCAAA 
GAGGAGAACT CGGCCTTCAT CTTCAAGCAG GAGGAGAAGC ACAGTAAAGG CTTATCAGCC 
GCCGGCGTGT ACGACGCTCC GTCTCGCCTC CCAGTGCAAA GCATCATTGC CAGCATGGGA 
ACTGTGGCCA TTGACCTGCA GAACCCGGAT CCACCGGCTG ACAGCAATAA ACTGGCTCTG 
CCCACGGAGA ACGGAGCGGG CAGCCGCAGA CCCAGCATTG CCCCTGTGCT GGAGATCGCA 
GACTCCTCTG CCATCCTGCC CTGTGATCTC CTCAACGACC CCTCAGAAGA CGAGACCAAC 
CAATCAGATG AGGATGGAGC GCCGGCCATA GACTGTGTTA AAGGCTATCC TCCAAACTCC 
CCTTACATTG GAAGCTCTCC AACACTTTGC CACCTTCTTC AACAGAAGGC ACCGTTTTGC 
TGTCTACGAC TTGACAAGGG TTGTCGACAC AATAGTTTCG AAGACGCCAA GGCGTATGGT 
TTCAAGAACA AGTTGATTAT CGTTTCTGCT GAGACAGCGG GAAATGGCTT GTACAACTTC 
ATAGTGCCGT TACGTGCCTA TTACCGACCG CGGAAGGAGC TGAATCCCAT TGTGCTGCTG 
CTGGATAATC CGCCTGATAA TCATTTCCTT GAGGCCATCT GCTGTTTCCC TATGGTGTAC 
TACATGGCGG GAACCATAGA CAATTTGGAT AACCTGCTGC AGTGTGGCAT CATCTACGCT 
GATAACCTGG TGGTGGTGGA TAAGGAGAGC ACTATGAGCG CAGAGGAAGA CTACATGGCA 
GACGCTAAAA CCATAGTCAA TGTGCAGACC ATGTTCAGGT TGTTTCCTAG TCTCAGCATA 
ATCACTGAGC TAACTCATCC GTCTAATATG AGGTTCATGC AGTTCCGTGC CAAAGACTGC 
TACTCCCTGG CTCTCTCCAA ACTCGAAAAG ATAGAGCGAG ATAAGGGATC GAATCTGGCG 
TTCATGTTCC GCCTGCCGTT CGCTGCAGGC AGAGTCTTCA GTATCAGCAT GCTGGATACT 
CTGCTTTACC AGTCGTTTGT GAAGGACTAT ATGATTCTCA TTGCGCGGCT CCTCCTGGGT 
TTAGACACCA CCCCGGGCTC AGGATACCTC TGTGCTATGA AGGTAACAGA AGAGGACTTA 
TGGATTCGAA CGTATGGGAG ACTCTTCCAG AAGCTGTGTT CCTCCAGCGC AGAGATTCCC 
ATCGGGATTT ACCGCACCGA ATCCCACATG TTCTCCTCTT CAGAGTCTCA GGTGTCTGTG 
GAGGATTGCG AGGACCAGCG GGACCACAGG GAATCCTGGA AAGAGAAAAC TGCCCATAGG 
AACTCCACAG GGAGCGACCA ATCAGAGCAT CCGCTCCTGC GCAAGAAAAG CATGCAGTGG 
GCGAGACGCC TAAGCAGAAA AAACACCAAA CCTCCCAGTC GAGCTGAACG CATCAATCAA 
CAGCGCCTGA ACCTGTACCG GCGCTCCGAG AGGCAGGAGC TGTCAGAGCT GGTGAAGAAC 
CGCATGAAGC ATTTAGGCCT GCCCACTGTG GGATACGAGG ACGTAGCTAA TTTAACCGCA 
AGCGATGTGA TGAACCGAGT AAATCTGGGA TATTTGCAAG ATGAAATGAA TGACAATCAG 
AACACGTTGT CATATGTGCT GATAAACCCT CCGCCGGACA CCATTCTGGA GCTCAATGAC 
ATTGTCTACA TCATCCGTTC TGATCCACTG GCACATGTCC CTGAAAATCT CCCAGGGGCT 
CCAGCTGGAG CGAAGATACG CCAAGACTAT CGGCCCGAGA CAAGGGATGA GACGCATCTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332353.1
CDS	703..4425
Translation	MMGPRSQSQAEPSMTPPRRSSTAADRLPTETVQRNNSSNTGVILDISTLKLADVDSEVPPLPPRYRFRDLLLGDQSFQNDDRVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNLSLKLLTCLLYIIRVSLDDPTNQVTCARKHCANITDALCSKACANYTANLTADPTQINWELIFWVNRRVPVWAIQVTVALISFLQAMLLVYLSYKGNIWEQIFQISFIIEMINTVPFIITIFWPPLRNIFVPVFLNCWLAKSALENMINDLHRAIQRTHSAMFNQVLILICTLLCLVFTGACGIQHLERAGKNLTLFDALYFCIVTFSTVGYGDVTPRIWPSQLLVVIMICVALVVLPLQFEELAYLWMERQKSGGNYSRHRAQTEKHVVLCVTSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRILQIPLWSQRVIYLQGSALKDQDLMRAKMDDAEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEEFKYAMLALNCVCPATSTLVTLLVHTSRGQEGQTSPEQWQRTYGRCSGNEVYHIRLMDSKFFGEYDGKSFTYASFHAHKKYGVCLIGTKREDNKSILLNPGPRHIMSATDTCYYINITKEENSAFIFKQEEKHSKGLSAAGVYDAPSRLPVQSIIASMGTVAIDLQNPDPPADSNKLALPTENGAGSRRPSIAPVLEIADSSAILPCDLLNDPSEDETNQSDEDGAPAIDCVKGYPPNSPYIGSSPTLCHLLQQKAPFCCLRLDKGCRHNSFEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRKELNPIVLLLDNPPDNHFLEAICCFPMVYYMAGTIDNLDNLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSIITELTHPSNMRFMQFRAKDCYSLALSKLEKIERDKGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMILIARLLLGLDTTPGSGYLCAMKVTEEDLWIRTYGRLFQKLCSSSAEIPIGIYRTESHMFSSSESQVSVEDCEDQRDHRESWKEKTAHRNSTGSDQSEHPLLRKKSMQWARRLSRKNTKPPSRAERINQQRLNLYRRSERQELSELVKNRMKHLGLPTVGYEDVANLTASDVMNRVNLGYLQDEMNDNQNTLSYVLINPPPDTILELNDIVYIIRSDPLAHVPENLPGAPAGAKIRQDYRPETRDETHL

Target ORF information:

RefSeq Version	XM_021476678.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:dkey-21e5.1 (si:dkey-21e5.1), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476678.1

ORF Insert Sequence:

ATGATGGGAC CGCGCAGTCA GTCTCAGGCA GAGCCAAGCA TGACTCCGCC ACGACGCTCT 
TCCACTGCTG CTGACAGGTT GCCTACGGAG ACCGTTCAGA GAAATAACAG CAGTAATACT 
GGTGTTATTC TGGATATTTC CACGCTGAAA TTGGCAGATG TGGACTCTGA GGTCCCTCCT 
CTACCTCCAC GATACCGCTT TCGAGATCTG CTGCTTGGGG ATCAGTCATT TCAGAACGAT 
GACAGGGTGC AGGTGGAGTT TTACGTAAAC GAAAACACAT TTAAGGAGAG GTTGAAGCTC 
TTCTTCATCA AAAACCAAAG GTCAAGTCTC AGAATACGCC TCTTTAACCT CTCCTTAAAG 
TTGTTAACAT GCCTGCTCTA CATAATCAGA GTCTCACTTG ATGATCCAAC TAACCAGGTC 
ACATGTGCAC GGAAACACTG CGCAAACATC ACAGATGCTC TGTGCAGCAA AGCCTGTGCC 
AACTATACAG CAAACCTGAC CGCAGATCCC ACACAAATAA ACTGGGAGTT AATATTTTGG 
GTGAATAGAA GAGTTCCTGT ATGGGCGATA CAGGTGACAG TGGCCTTAAT TAGCTTCTTG 
CAAGCGATGC TCCTGGTGTA TCTCAGCTAC AAGGGCAACA TTTGGGAACA GATTTTCCAA 
ATCTCCTTCA TCATTGAGAT GATCAATACA GTGCCATTCA TAATTACAAT CTTTTGGCCT 
CCGCTTAGGA ACATATTTGT TCCTGTATTT CTTAACTGTT GGCTTGCCAA ATCAGCGCTG 
GAGAATATGA TTAATGATCT CCATCGTGCC ATCCAGCGCA CACACTCTGC CATGTTCAAC 
CAGGTCCTCA TCCTGATCTG TACACTGCTC TGTCTGGTCT TCACCGGGGC TTGTGGGATT 
CAGCACCTGG AGCGAGCAGG GAAGAATCTG ACACTCTTTG ACGCTTTATA TTTCTGCATC 
GTCACCTTCT CCACTGTTGG ATATGGAGAT GTGACGCCCA GAATCTGGCC CTCTCAGCTG 
CTGGTGGTCA TCATGATCTG TGTGGCGCTG GTGGTGCTGC CATTACAGTT CGAGGAGTTG 
GCGTATCTAT GGATGGAGAG GCAGAAGTCA GGTGGAAACT ACAGCAGACA CCGCGCTCAG 
ACAGAGAAAC ACGTGGTGCT CTGCGTCACC TCACTCAAAA TAGACCTGCT CATGGATTTC 
CTCAATGAGT TCTATGCCCA CCCGAGACTA CAGGACTATT ATGTGGTGAT CCTCTGTCCC 
ACGGAGATGG ACATCCAGGT GCGGCGAATA CTGCAGATCC CACTGTGGTC TCAGCGGGTC 
ATCTACCTCC AAGGCTCTGC ACTGAAGGAC CAAGACCTCA TGAGGGCCAA GATGGATGAT 
GCGGAGGCCT GCTTCATCCT CAGCAGCAGA AATGAAGTGG ACCGCACCGC TGCTGATCAT 
CAGACCATTC TGCGAGCCTG GGCTGTGAAG GATTTTGCTC CAAACTGTCC TCTTTATGTC 
CAGATCCTCA AACCCGAGAA CAAATTTCAT GTCAAATTTG CAGATCACGT GGTGTGTGAA 
GAGGAGTTCA AGTACGCCAT GCTGGCCCTG AACTGTGTGT GTCCCGCCAC CTCCACACTG 
GTCACCTTGT TGGTGCACAC GTCTCGAGGA CAAGAAGGTC AGACGTCTCC AGAACAGTGG 
CAGAGGACTT ACGGTCGCTG CTCGGGGAAT GAAGTTTATC ACATCCGTTT GATGGACAGC 
AAGTTTTTTG GAGAATATGA TGGGAAGAGT TTCACTTATG CCTCCTTCCA CGCACACAAG 
AAGTATGGTG TGTGTCTAAT CGGCACAAAG AGAGAAGACA ACAAGAGCAT TCTCCTGAAC 
CCCGGCCCTC GCCACATCAT GTCCGCCACA GACACCTGCT ACTACATCAA CATCACCAAA 
GAGGAGAACT CGGCCTTCAT CTTCAAGCAG GAGGAGAAGC ACAGTAAAGG CTTATCAGCC 
GCCGGCGTGT ACGACGCTCC GTCTCGCCTC CCAGTGCAAA GCATCATTGC CAGCATGGGA 
ACTGTGGCCA TTGACCTGCA GAACCCGGAT CCACCGGCTG ACAGCAATAA ACTGGCTCTG 
CCCACGGAGA ACGGAGCGGG CAGCCGCAGA CCCAGCATTG CCCCTGTGCT GGAGATCGCA 
GACTCCTCTG CCATCCTGCC CTGTGATCTC CTCAACGACC CCTCAGAAGA CGAGACCAAC 
CAATCAGATG AGGATGGAGC GCCGGCCATA GACTGTGTTA AAGGCTATCC TCCAAACTCC 
CCTTACATTG GAAGCTCTCC AACACTTTGC CACCTTCTTC AACAGAAGGC ACCGTTTTGC 
TGTCTACGAC TTGACAAGGG TTGTCGACAC AATAGTTTCG AAGACGCCAA GGCGTATGGT 
TTCAAGAACA AGTTGATTAT CGTTTCTGCT GAGACAGCGG GAAATGGCTT GTACAACTTC 
ATAGTGCCGT TACGTGCCTA TTACCGACCG CGGAAGGAGC TGAATCCCAT TGTGCTGCTG 
CTGGATAATC CGCCTGATAA TCATTTCCTT GAGGCCATCT GCTGTTTCCC TATGGTGTAC 
TACATGGCGG GAACCATAGA CAATTTGGAT AACCTGCTGC AGTGTGGCAT CATCTACGCT 
GATAACCTGG TGGTGGTGGA TAAGGAGAGC ACTATGAGCG CAGAGGAAGA CTACATGGCA 
GACGCTAAAA CCATAGTCAA TGTGCAGACC ATGTTCAGGT TGTTTCCTAG TCTCAGCATA 
ATCACTGAGC TAACTCATCC GTCTAATATG AGGTTCATGC AGTTCCGTGC CAAAGACTGC 
TACTCCCTGG CTCTCTCCAA ACTCGAAAAG ATAGAGCGAG ATAAGGGATC GAATCTGGCG 
TTCATGTTCC GCCTGCCGTT CGCTGCAGGC AGAGTCTTCA GTATCAGCAT GCTGGATACT 
CTGCTTTACC AGTCGTTTGT GAAGGACTAT ATGATTCTCA TTGCGCGGCT CCTCCTGGGT 
TTAGACACCA CCCCGGGCTC AGGATACCTC TGTGCTATGA AGGTAACAGA AGAGGACTTA 
TGGATTCGAA CGTATGGGAG ACTCTTCCAG AAGCTGTGTT CCTCCAGCGC AGAGATTCCC 
ATCGGGATTT ACCGCACCGA ATCCCACATG TTCTCCTCTT CAGAGTCTCA GGTGTCTGTG 
GAGGATTGCG AGGACCAGCG GGACCACAGG GAATCCTGGA AAGAGAAAAC TGCCCATAGG 
AACTCCACAG GGAGCGACCA ATCAGAGCAT CCGCTCCTGC GCAAGAAAAG CATGCAGTGG 
GCGAGACGCC TAAGCAGAAA AAACACCAAA CCTCCCAGTC GAGCTGAACG CATCAATCAA 
CAGCGCCTGA ACCTGTACCG GCGCTCCGAG AGGCAGGAGC TGTCAGAGCT GGTGAAGAAC 
CGCATGAAGC ATTTAGGCCT GCCCACTGTG GGATACGAGG ACGTAGCTAA TTTAACCGCA 
AGCGATGTGA TGAACCGAGT AAATCTGGGA TATTTGCAAG ATGAAATGAA TGACAATCAG 
AACACGTTGT CATATGTGCT GATAAACCCT CCGCCGGACA CCATTCTGGA GCTCAATGAC 
ATTGTCTACA TCATCCGTTC TGATCCACTG GCACATGTCC CTGAAAATCT CCCAGGGGCT 
CCAGCTGGAG CGAAGATACG CCAAGACTAT CGGCCCGAGA CAAGGGATGA GACGCATCTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:dkey-21e5.1 ( XM_021476674.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021332349.1 Danio rerio si:dkey-21e5.1 (si:dkey-21e5.1), transcript variant X2, mRNA.

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CloneID	ODa44133
Clone ID Related Accession (Same CDS sequence)	XM_021476674.1
Accession Version	XM_021476674.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3783bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	potassium channel subfamily T member 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGAC CGCGCAGTCA GTCTCAGGCA GAGCCAAGCA TGACTCCGCC ACGACGCTCT 
TCCACTGCTG CTGACAGGTT GCCTACGGAG ACCGTTCAGA GAAATAACAG CAGTAATACT 
GGTGTTATTC TGGATATTTC CACGCTGAAA TTGGCAGATG TGGACTCTGA GGTCCCTCCT 
CTACCTCCAC GATACCGCTT TCGAGATCTG CTGCTTGGGG ATCAGTCATT TCAGAACGAT 
GACAGGGTGC AGGTGGAGTT TTACGTAAAC GAAAACACAT TTAAGGAGAG GTTGAAGCTC 
TTCTTCATCA AAAACCAAAG GTCAAGTCTC AGAATACGCC TCTTTAACCT CTCCTTAAAG 
TTGTTAACAT GCCTGCTCTA CATAATCAGA GTCTCACTTG ATGATCCAAC TAACCAGGTC 
ACATGTGCAC GGAAACACTG CGCAAACATC ACAGATGCTC TGTGCAGCAA AGCCTGTGCC 
AACTATACAG CAAACCTGAC CGCAGATCCC ACACAAATAA ACTGGGAGTT AATATTTTGG 
GTGAATAGAA GAGTTCCTGT ATGGGCGATA CAGGTGACAG TGGCCTTAAT TAGCTTCTTG 
CAAGCGATGC TCCTGGTGTA TCTCAGCTAC AAGGGCAACA TTTGGGAACA GATTTTCCAA 
ATCTCCTTCA TCATTGAGAT GATCAATACA GTGCCATTCA TAATTACAAT CTTTTGGCCT 
CCGCTTAGGA ACATATTTGT TCCTGTATTT CTTAACTGTT GGCTTGCCAA ATCAGCGCTG 
GAGAATATGA TTAATGATCT CCATCGTGCC ATCCAGCGCA CACACTCTGC CATGTTCAAC 
CAGGTCCTCA TCCTGATCTG TACACTGCTC TGTCTGGTCT TCACCGGGGC TTGTGGGATT 
CAGCACCTGG AGCGAGCAGG GAAGAATCTG ACACTCTTTG ACGCTTTATA TTTCTGCATC 
GTCACCTTCT CCACTGTTGG ATATGGAGAT GTGACGCCCA GAATCTGGCC CTCTCAGCTG 
CTGGTGGTCA TCATGATCTG TGTGGCGCTG GTGGTGCTGC CATTACAGTT CGAGGAGTTG 
GCGTATCTAT GGATGGAGAG GCAGAAGTCA GGTGGAAACT ACAGCAGACA CCGCGCTCAG 
ACAGAGAAAC ACGTGGTGCT CTGCGTCACC TCACTCAAAA TAGACCTGCT CATGGATTTC 
CTCAATGAGT TCTATGCCCA CCCGAGACTA CAGGACTATT ATGTGGTGAT CCTCTGTCCC 
ACGGAGATGG ACATCCAGGT GCGGCGAATA CTGCAGATCC CACTGTGGTC TCAGCGGGTC 
ATCTACCTCC AAGGCTCTGC ACTGAAGGAC CAAGACCTCA TGAGGGCCAA GATGGATGAT 
GCGGAGGCCT GCTTCATCCT CAGCAGCAGA AATGAAGTGG ACCGCACCGC TGCTGATCAT 
CAGACCATTC TGCGAGCCTG GGCTGTGAAG GATTTTGCTC CAAACTGTCC TCTTTATGTC 
CAGATCCTCA AACCCGAGAA CAAATTTCAT GTCAAATTTG CAGGTATGTT TTGTTTGCTA 
GATCACGTGG TGTGTGAAGA GGAGTTCAAG TACGCCATGC TGGCCCTGAA CTGTGTGTGT 
CCCGCCACCT CCACACTGGT CACCTTGTTG GTGCACACGT CTCGAGGACA AGAAGGTCAG 
ACGTCTCCAG AACAGTGGCA GAGGACTTAC GGTCGCTGCT CGGGGAATGA AGTTTATCAC 
ATCCGTTTGA TGGACAGCAA GTTTTTTGGA GAATATGATG GGAAGAGTTT CACTTATGCC 
TCCTTCCACG CACACAAGAA GTATGGTGTG TGTCTAATCG GCACAAAGAG AGAAGACAAC 
AAGAGCATTC TCCTGAACCC CGGCCCTCGC CACATCATGT CCGCCACAGA CACCTGCTAC 
TACATCAACA TCACCAAAGA GGAGAACTCG GCCTTCATCT TCAAGCAGGA GGAGAAGCAC 
AGTAAAGGCT TATCAGCCGC CGGCGTGTAC GACGCTCCGT CTCGCCTCCC AGTGCAAAGC 
ATCATTGCCA GCATGGGAAC TGTGGCCATT GACCTGCAGA ACCCGGATCC ACCGGCTGAC 
AGCAATAAAC TGGCTCTGCC CACGGAGAAC GGAGCGGGCA GCCGCAGACC CAGCATTGCC 
CCTGTGCTGG AGATCGCAGA CTCCTCTGCC ATCCTGCCCT GTGATCTCCT CAACGACCCC 
TCAGAAGACG AGACCAACCA ATCAGATGAG GATGGAGCGC CGGCCATAGA CTGTGTTAAA 
GGCTATCCTC CAAACTCCCC TTACATTGGA AGCTCTCCAA CACTTTGCCA CCTTCTTCAA 
CAGAAGGCAC CGTTTTGCTG TCTACGACTT GACAAGGGTT GTCGACACAA TAGTTTCGAA 
GACGCCAAGG CGTATGGTTT CAAGAACAAG TTGATTATCG TTTCTGCTGA GACAGCGGGA 
AATGGCTTGT ACAACTTCAT AGTGCCGTTA CGTGCCTATT ACCGACCGCG GAAGGAGCTG 
AATCCCATTG TGCTGCTGCT GGATAATCCG CCTGATAATC ATTTCCTTGA GGCCATCTGC 
TGTTTCCCTA TGGTGTACTA CATGGCGGGA ACCATAGACA ATTTGGATAA CCTGCTGCAG 
TGTGGCATCA TCTACGCTGA TAACCTGGTG GTGGTGGATA AGGAGAGCAC TATGAGCGCA 
GAGGAAGACT ACATGGCAGA CGCTAAAACC ATAGTCAATG TGCAGACCAT GTTCAGGTTG 
TTTCCTAGTC TCAGCATAAT CACTGAGCTA ACTCATCCGT CTAATATGAG GTTCATGCAG 
TTCCGTGCCA AAGACTGCTA CTCCCTGGCT CTCTCCAAAC TCGAAAAGAT AGAGCGAGAT 
AAGGGATCGA ATCTGGCGTT CATGTTCCGC CTGCCGTTCG CTGCAGGCAG AGTCTTCAGT 
ATCAGCATGC TGGATACTCT GCTTTACCAG TCGTTTGTGA AGGACTATAT GATTCTCATT 
GCGCGGCTCC TCCTGGGTTT AGACACCACC CCGGGCTCAG GATACCTCTG TGCTATGAAG 
GTAACAGAAG AGGACTTATG GATTCGAACG TATGGGAGAC TCTTCCAGAA GCTGTGTTCC 
TCCAGCGCAG AGATTCCCAT CGGGATTTAC CGCACCGAAT CCCACATGTT CTCCTCTTCA 
GAGTCTCAGG TGTCTGTGGA GGATTGCGAG GACCAGCGGG ACCACAGGGA ATCCTGGAAA 
GAGAAAACTG CCCATAGGAA CTCCACAGGG AGCGACCAAT CAGAGCATCC GCTCCTGCGC 
AAGAAAAGCA TGCAGTGGGC GAGACGCCTA AGCAGAAAAA ACACCAAACC TCCCAGTCGA 
GCTGAACGCA TCAATCAACA GCGCCTGAAC CTGTACCGGC GCTCCGAGAG GCAGGAGCTG 
TCAGAGCTGG TGAAGAACCG CATGAAGCAT TTAGGCCTGC CCACTGTGGG ATACGAGGAC 
GTAGCTAATT TAACCGCAAG CGATGTGATG AACCGAGTAA ATCTGGGATA TTTGCAAGAG 
GGTACTTACA TGACAGAGGG TACTGCAGTG GCTCAAACAG ATGAAATGAA TGACAATCAG 
AACACGTTGT CATATGTGCT GATAAACCCT CCGCCGGACA CCATTCTGGA GCTCAATGAC 
ATTGTCTACA TCATCCGTTC TGATCCACTG GCACATGTCC CTGAAAATCT CCCAGGGGCT 
CCAGCTGGAG CGAAGATACG CCAAGACTAT CGGCCCGAGA CAAGGGATGA GACGCATCTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021332349.1
CDS	703..4485
Translation	MMGPRSQSQAEPSMTPPRRSSTAADRLPTETVQRNNSSNTGVILDISTLKLADVDSEVPPLPPRYRFRDLLLGDQSFQNDDRVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNLSLKLLTCLLYIIRVSLDDPTNQVTCARKHCANITDALCSKACANYTANLTADPTQINWELIFWVNRRVPVWAIQVTVALISFLQAMLLVYLSYKGNIWEQIFQISFIIEMINTVPFIITIFWPPLRNIFVPVFLNCWLAKSALENMINDLHRAIQRTHSAMFNQVLILICTLLCLVFTGACGIQHLERAGKNLTLFDALYFCIVTFSTVGYGDVTPRIWPSQLLVVIMICVALVVLPLQFEELAYLWMERQKSGGNYSRHRAQTEKHVVLCVTSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRILQIPLWSQRVIYLQGSALKDQDLMRAKMDDAEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFAGMFCLLDHVVCEEEFKYAMLALNCVCPATSTLVTLLVHTSRGQEGQTSPEQWQRTYGRCSGNEVYHIRLMDSKFFGEYDGKSFTYASFHAHKKYGVCLIGTKREDNKSILLNPGPRHIMSATDTCYYINITKEENSAFIFKQEEKHSKGLSAAGVYDAPSRLPVQSIIASMGTVAIDLQNPDPPADSNKLALPTENGAGSRRPSIAPVLEIADSSAILPCDLLNDPSEDETNQSDEDGAPAIDCVKGYPPNSPYIGSSPTLCHLLQQKAPFCCLRLDKGCRHNSFEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRKELNPIVLLLDNPPDNHFLEAICCFPMVYYMAGTIDNLDNLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSIITELTHPSNMRFMQFRAKDCYSLALSKLEKIERDKGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMILIARLLLGLDTTPGSGYLCAMKVTEEDLWIRTYGRLFQKLCSSSAEIPIGIYRTESHMFSSSESQVSVEDCEDQRDHRESWKEKTAHRNSTGSDQSEHPLLRKKSMQWARRLSRKNTKPPSRAERINQQRLNLYRRSERQELSELVKNRMKHLGLPTVGYEDVANLTASDVMNRVNLGYLQEGTYMTEGTAVAQTDEMNDNQNTLSYVLINPPPDTILELNDIVYIIRSDPLAHVPENLPGAPAGAKIRQDYRPETRDETHL

Target ORF information:

RefSeq Version	XM_021476674.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio si:dkey-21e5.1 (si:dkey-21e5.1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476674.1

ORF Insert Sequence:

ATGATGGGAC CGCGCAGTCA GTCTCAGGCA GAGCCAAGCA TGACTCCGCC ACGACGCTCT 
TCCACTGCTG CTGACAGGTT GCCTACGGAG ACCGTTCAGA GAAATAACAG CAGTAATACT 
GGTGTTATTC TGGATATTTC CACGCTGAAA TTGGCAGATG TGGACTCTGA GGTCCCTCCT 
CTACCTCCAC GATACCGCTT TCGAGATCTG CTGCTTGGGG ATCAGTCATT TCAGAACGAT 
GACAGGGTGC AGGTGGAGTT TTACGTAAAC GAAAACACAT TTAAGGAGAG GTTGAAGCTC 
TTCTTCATCA AAAACCAAAG GTCAAGTCTC AGAATACGCC TCTTTAACCT CTCCTTAAAG 
TTGTTAACAT GCCTGCTCTA CATAATCAGA GTCTCACTTG ATGATCCAAC TAACCAGGTC 
ACATGTGCAC GGAAACACTG CGCAAACATC ACAGATGCTC TGTGCAGCAA AGCCTGTGCC 
AACTATACAG CAAACCTGAC CGCAGATCCC ACACAAATAA ACTGGGAGTT AATATTTTGG 
GTGAATAGAA GAGTTCCTGT ATGGGCGATA CAGGTGACAG TGGCCTTAAT TAGCTTCTTG 
CAAGCGATGC TCCTGGTGTA TCTCAGCTAC AAGGGCAACA TTTGGGAACA GATTTTCCAA 
ATCTCCTTCA TCATTGAGAT GATCAATACA GTGCCATTCA TAATTACAAT CTTTTGGCCT 
CCGCTTAGGA ACATATTTGT TCCTGTATTT CTTAACTGTT GGCTTGCCAA ATCAGCGCTG 
GAGAATATGA TTAATGATCT CCATCGTGCC ATCCAGCGCA CACACTCTGC CATGTTCAAC 
CAGGTCCTCA TCCTGATCTG TACACTGCTC TGTCTGGTCT TCACCGGGGC TTGTGGGATT 
CAGCACCTGG AGCGAGCAGG GAAGAATCTG ACACTCTTTG ACGCTTTATA TTTCTGCATC 
GTCACCTTCT CCACTGTTGG ATATGGAGAT GTGACGCCCA GAATCTGGCC CTCTCAGCTG 
CTGGTGGTCA TCATGATCTG TGTGGCGCTG GTGGTGCTGC CATTACAGTT CGAGGAGTTG 
GCGTATCTAT GGATGGAGAG GCAGAAGTCA GGTGGAAACT ACAGCAGACA CCGCGCTCAG 
ACAGAGAAAC ACGTGGTGCT CTGCGTCACC TCACTCAAAA TAGACCTGCT CATGGATTTC 
CTCAATGAGT TCTATGCCCA CCCGAGACTA CAGGACTATT ATGTGGTGAT CCTCTGTCCC 
ACGGAGATGG ACATCCAGGT GCGGCGAATA CTGCAGATCC CACTGTGGTC TCAGCGGGTC 
ATCTACCTCC AAGGCTCTGC ACTGAAGGAC CAAGACCTCA TGAGGGCCAA GATGGATGAT 
GCGGAGGCCT GCTTCATCCT CAGCAGCAGA AATGAAGTGG ACCGCACCGC TGCTGATCAT 
CAGACCATTC TGCGAGCCTG GGCTGTGAAG GATTTTGCTC CAAACTGTCC TCTTTATGTC 
CAGATCCTCA AACCCGAGAA CAAATTTCAT GTCAAATTTG CAGGTATGTT TTGTTTGCTA 
GATCACGTGG TGTGTGAAGA GGAGTTCAAG TACGCCATGC TGGCCCTGAA CTGTGTGTGT 
CCCGCCACCT CCACACTGGT CACCTTGTTG GTGCACACGT CTCGAGGACA AGAAGGTCAG 
ACGTCTCCAG AACAGTGGCA GAGGACTTAC GGTCGCTGCT CGGGGAATGA AGTTTATCAC 
ATCCGTTTGA TGGACAGCAA GTTTTTTGGA GAATATGATG GGAAGAGTTT CACTTATGCC 
TCCTTCCACG CACACAAGAA GTATGGTGTG TGTCTAATCG GCACAAAGAG AGAAGACAAC 
AAGAGCATTC TCCTGAACCC CGGCCCTCGC CACATCATGT CCGCCACAGA CACCTGCTAC 
TACATCAACA TCACCAAAGA GGAGAACTCG GCCTTCATCT TCAAGCAGGA GGAGAAGCAC 
AGTAAAGGCT TATCAGCCGC CGGCGTGTAC GACGCTCCGT CTCGCCTCCC AGTGCAAAGC 
ATCATTGCCA GCATGGGAAC TGTGGCCATT GACCTGCAGA ACCCGGATCC ACCGGCTGAC 
AGCAATAAAC TGGCTCTGCC CACGGAGAAC GGAGCGGGCA GCCGCAGACC CAGCATTGCC 
CCTGTGCTGG AGATCGCAGA CTCCTCTGCC ATCCTGCCCT GTGATCTCCT CAACGACCCC 
TCAGAAGACG AGACCAACCA ATCAGATGAG GATGGAGCGC CGGCCATAGA CTGTGTTAAA 
GGCTATCCTC CAAACTCCCC TTACATTGGA AGCTCTCCAA CACTTTGCCA CCTTCTTCAA 
CAGAAGGCAC CGTTTTGCTG TCTACGACTT GACAAGGGTT GTCGACACAA TAGTTTCGAA 
GACGCCAAGG CGTATGGTTT CAAGAACAAG TTGATTATCG TTTCTGCTGA GACAGCGGGA 
AATGGCTTGT ACAACTTCAT AGTGCCGTTA CGTGCCTATT ACCGACCGCG GAAGGAGCTG 
AATCCCATTG TGCTGCTGCT GGATAATCCG CCTGATAATC ATTTCCTTGA GGCCATCTGC 
TGTTTCCCTA TGGTGTACTA CATGGCGGGA ACCATAGACA ATTTGGATAA CCTGCTGCAG 
TGTGGCATCA TCTACGCTGA TAACCTGGTG GTGGTGGATA AGGAGAGCAC TATGAGCGCA 
GAGGAAGACT ACATGGCAGA CGCTAAAACC ATAGTCAATG TGCAGACCAT GTTCAGGTTG 
TTTCCTAGTC TCAGCATAAT CACTGAGCTA ACTCATCCGT CTAATATGAG GTTCATGCAG 
TTCCGTGCCA AAGACTGCTA CTCCCTGGCT CTCTCCAAAC TCGAAAAGAT AGAGCGAGAT 
AAGGGATCGA ATCTGGCGTT CATGTTCCGC CTGCCGTTCG CTGCAGGCAG AGTCTTCAGT 
ATCAGCATGC TGGATACTCT GCTTTACCAG TCGTTTGTGA AGGACTATAT GATTCTCATT 
GCGCGGCTCC TCCTGGGTTT AGACACCACC CCGGGCTCAG GATACCTCTG TGCTATGAAG 
GTAACAGAAG AGGACTTATG GATTCGAACG TATGGGAGAC TCTTCCAGAA GCTGTGTTCC 
TCCAGCGCAG AGATTCCCAT CGGGATTTAC CGCACCGAAT CCCACATGTT CTCCTCTTCA 
GAGTCTCAGG TGTCTGTGGA GGATTGCGAG GACCAGCGGG ACCACAGGGA ATCCTGGAAA 
GAGAAAACTG CCCATAGGAA CTCCACAGGG AGCGACCAAT CAGAGCATCC GCTCCTGCGC 
AAGAAAAGCA TGCAGTGGGC GAGACGCCTA AGCAGAAAAA ACACCAAACC TCCCAGTCGA 
GCTGAACGCA TCAATCAACA GCGCCTGAAC CTGTACCGGC GCTCCGAGAG GCAGGAGCTG 
TCAGAGCTGG TGAAGAACCG CATGAAGCAT TTAGGCCTGC CCACTGTGGG ATACGAGGAC 
GTAGCTAATT TAACCGCAAG CGATGTGATG AACCGAGTAA ATCTGGGATA TTTGCAAGAG 
GGTACTTACA TGACAGAGGG TACTGCAGTG GCTCAAACAG ATGAAATGAA TGACAATCAG 
AACACGTTGT CATATGTGCT GATAAACCCT CCGCCGGACA CCATTCTGGA GCTCAATGAC 
ATTGTCTACA TCATCCGTTC TGATCCACTG GCACATGTCC CTGAAAATCT CCCAGGGGCT 
CCAGCTGGAG CGAAGATACG CCAAGACTAT CGGCCCGAGA CAAGGGATGA GACGCATCTG 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

si:dkey-21e5.1 ( XM_017356387.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211876.1 Danio rerio si:dkey-21e5.1 (si:dkey-21e5.1), mRNA.

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CloneID	ODa59308
Clone ID Related Accession (Same CDS sequence)	XM_017356387.1
Accession Version	XM_017356387.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3759bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	potassium channel subfamily T member 1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGGAC CGCGCAGTCA GTCTCAGGCA GAGCCAAGCA TGACTCCGCC ACGACGCTCT 
TCCACTGCTG CTGACAGGTT GCCTACGGAG ACCGTTCAGA GAAATAACAG CAGTAATACT 
GGTGTTATTC TGGATATTTC CACGCTGAAA TTGGCAGATG TGGACTCTGA GGTCCCTCCT 
CTACCTCCAC GATACCGCTT TCGAGATCTG CTGCTTGGGG ATCAGTCATT TCAGAACGAT 
GACAGGGTGC AGGTGGAGTT TTACGTAAAC GAAAACACAT TTAAGGAGAG GTTGAAGCTC 
TTCTTCATCA AAAACCAAAG GTCAAGTCTC AGAATACGCC TCTTTAACCT CTCCTTAAAG 
TTGTTAACAT GCCTGCTCTA CATAATCAGA GTCTCACTTG ATGATCCAAC TAACCAGGTC 
ACATGTGCAC GGAAACACTG CGCAAACATC ACAGATGCTC TGTGCAGCAA AGCCTGTGCC 
AACTATACAG CAAACCTGAC CGCAGATCCC ACACAAATAA ACTGGGAGTT AATATTTTGG 
GTGAATAGAA GAGTTCCTGT ATGGGCGATA CAGGTGACAG TGGCCTTAAT TAGCTTCTTG 
CAAGCGATGC TCCTGGTGTA TCTCAGCTAC AAGGGCAACA TTTGGGAACA GATTTTCCAA 
ATCTCCTTCA TCATTGAGAT GATCAATACA GTGCCATTCA TAATTACAAT CTTTTGGCCT 
CCGCTTAGGA ACATATTTGT TCCTGTATTT CTTAACTGTT GGCTTGCCAA ATCAGCGCTG 
GAGAATATGA TTAATGATCT CCATCGTGCC ATCCAGCGCA CACACTCTGC CATGTTCAAC 
CAGGTCCTCA TCCTGATCTG TACACTGCTC TGTCTGGTCT TCACCGGGGC TTGTGGGATT 
CAGCACCTGG AGCGAGCAGG GAAGAATCTG ACACTCTTTG ACGCTTTATA TTTCTGCATC 
GTCACCTTCT CCACTGTTGG ATATGGAGAT GTGACGCCCA GAATCTGGCC CTCTCAGCTG 
CTGGTGGTCA TCATGATCTG TGTGGCGCTG GTGGTGCTGC CATTACAGTT CGAGGAGTTG 
GCGTATCTAT GGATGGAGAG GCAGAAGTCA GGTGGAAACT ACAGCAGACA CCGCGCTCAG 
ACAGAGAAAC ACGTGGTGCT CTGCGTCACC TCACTCAAAA TAGACCTGCT CATGGATTTC 
CTCAATGAGT TCTATGCCCA CCCGAGACTA CAGGACTATT ATGTGGTGAT CCTCTGTCCC 
ACGGAGATGG ACATCCAGGT GCGGCGAATA CTGCAGATCC CACTGTGGTC TCAGCGGGTC 
ATCTACCTCC AAGGCTCTGC ACTGAAGGAC CAAGACCTCA TGAGGGCCAA GATGGATGAT 
GCGGAGGCCT GCTTCATCCT CAGCAGCAGA AATGAAGTGG ACCGCACCGC TGCTGATCAT 
CAGACCATTC TGCGAGCCTG GGCTGTGAAG GATTTTGCTC CAAACTGTCC TCTTTATGTC 
CAGATCCTCA AACCCGAGAA CAAATTTCAT GTCAAATTTG CAGATCACGT GGTGTGTGAA 
GAGGAGTTCA AGTACGCCAT GCTGGCCCTG AACTGTGTGT GTCCCGCCAC CTCCACACTG 
GTCACCTTGT TGGTGCACAC GTCTCGAGGA CAAGAAGGTC AGACGTCTCC AGAACAGTGG 
CAGAGGACTT ACGGTCGCTG CTCGGGGAAT GAAGTTTATC ACATCCGTTT GATGGACAGC 
AAGTTTTTTG GAGAATATGA TGGGAAGAGT TTCACTTATG CCTCCTTCCA CGCACACAAG 
AAGTATGGTG TGTGTCTAAT CGGCACAAAG AGAGAAGACA ACAAGAGCAT TCTCCTGAAC 
CCCGGCCCTC GCCACATCAT GTCCGCCACA GACACCTGCT ACTACATCAA CATCACCAAA 
GAGGAGAACT CGGCCTTCAT CTTCAAGCAG GAGGAGAAGC ACAGTAAAGG CTTATCAGCC 
GCCGGCGTGT ACGACGCTCC GTCTCGCCTC CCAGTGCAAA GCATCATTGC CAGCATGGGA 
ACTGTGGCCA TTGACCTGCA GAACCCGGAT CCACCGGCTG ACAGCAATAA ACTGGCTCTG 
CCCACGGAGA ACGGAGCGGG CAGCCGCAGA CCCAGCATTG CCCCTGTGCT GGAGATCGCA 
GACTCCTCTG CCATCCTGCC CTGTGATCTC CTCAACGACC CCTCAGAAGA CGAGACCAAC 
CAATCAGATG AGGATGGAGC GCCGGCCATA GACTGTGTTA AAGGCTATCC TCCAAACTCC 
CCTTACATTG GAAGCTCTCC AACACTTTGC CACCTTCTTC AACAGAAGGC ACCGTTTTGC 
TGTCTACGAC TTGACAAGGG TTGTCGACAC AATAGTTTCG AAGACGCCAA GGCGTATGGT 
TTCAAGAACA AGTTGATTAT CGTTTCTGCT GAGACAGCGG GAAATGGCTT GTACAACTTC 
ATAGTGCCGT TACGTGCCTA TTACCGACCG CGGAAGGAGC TGAATCCCAT TGTGCTGCTG 
CTGGATAATC CGCCTGATAA TCATTTCCTT GAGGCCATCT GCTGTTTCCC TATGGTGTAC 
TACATGGCGG GAACCATAGA CAATTTGGAT AACCTGCTGC AGTGTGGCAT CATCTACGCT 
GATAACCTGG TGGTGGTGGA TAAGGAGAGC ACTATGAGCG CAGAGGAAGA CTACATGGCA 
GACGCTAAAA CCATAGTCAA TGTGCAGACC ATGTTCAGGT TGTTTCCTAG TCTCAGCATA 
ATCACTGAGC TAACTCATCC GTCTAATATG AGGTTCATGC AGTTCCGTGC CAAAGACTGC 
TACTCCCTGG CTCTCTCCAA ACTCGAAAAG ATAGAGCGAG ATAAGGGATC GAATCTGGCG 
TTCATGTTCC GCCTGCCGTT CGCTGCAGGC AGAGTCTTCA GTATCAGCAT GCTGGATACT 
CTGCTTTACC AGTCGTTTGT GAAGGACTAT ATGATTCTCA TTGCGCGGCT CCTCCTGGGT 
TTAGACACCA CCCCGGGCTC AGGATACCTC TGTGCTATGA AGGTAACAGA AGAGGACTTA 
TGGATTCGAA CGTATGGGAG ACTCTTCCAG AAGCTGTGTT CCTCCAGCGC AGAGATTCCC 
ATCGGGATTT ACCGCACCGA ATCCCACATG TTCTCCTCTT CAGAGGTGTC TGTGGAGGAT 
TGCGAGGACC AGCGGGACCA CAGGGAATCC TGGAAAGAGA AAACTGCCCA TAGGAACTCC 
ACAGGGAGCG ACCAATCAGA GCATCCGCTC CTGCGCAAGA AAAGCATGCA GTGGGCGAGA 
CGCCTAAGCA GAAAAAACAC CAAACCTCCC AGTCGAGCTG AACGCATCAA TCAACAGCGC 
CTGAACCTGT ACCGGCGCTC CGAGAGGCAG GAGCTGTCAG AGCTGGTGAA GAACCGCATG 
AAGCATTTAG GCCTGCCCAC TGTGGGATAC GAGGACGTAG CTAATTTAAC CGCAAGCGAT 
GTGATGAACC GAGTAAATCT GGGATATTTG CAAGAGGGTA CTTACATGAC AGAGGGTACT 
GCAGTGGCTC AAACAGATGA AATGAATGAC AATCAGAACA CGTTGTCATA TGTGCTGATA 
AACCCTCCGC CGGACACCAT TCTGGAGCTC AATGACATTG TCTACATCAT CCGTTCTGAT 
CCACTGGCAC ATGTCCCTGA AAATCTCCCA GGGGCTCCAG CTGGAGCGAA GATACGCCAA 
GACTATCGGC CCGAGACAAG GGATGAGACG CATCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211876.1
CDS	506..4264
Translation	MMGPRSQSQAEPSMTPPRRSSTAADRLPTETVQRNNSSNTGVILDISTLKLADVDSEVPPLPPRYRFRDLLLGDQSFQNDDRVQVEFYVNENTFKERLKLFFIKNQRSSLRIRLFNLSLKLLTCLLYIIRVSLDDPTNQVTCARKHCANITDALCSKACANYTANLTADPTQINWELIFWVNRRVPVWAIQVTVALISFLQAMLLVYLSYKGNIWEQIFQISFIIEMINTVPFIITIFWPPLRNIFVPVFLNCWLAKSALENMINDLHRAIQRTHSAMFNQVLILICTLLCLVFTGACGIQHLERAGKNLTLFDALYFCIVTFSTVGYGDVTPRIWPSQLLVVIMICVALVVLPLQFEELAYLWMERQKSGGNYSRHRAQTEKHVVLCVTSLKIDLLMDFLNEFYAHPRLQDYYVVILCPTEMDIQVRRILQIPLWSQRVIYLQGSALKDQDLMRAKMDDAEACFILSSRNEVDRTAADHQTILRAWAVKDFAPNCPLYVQILKPENKFHVKFADHVVCEEEFKYAMLALNCVCPATSTLVTLLVHTSRGQEGQTSPEQWQRTYGRCSGNEVYHIRLMDSKFFGEYDGKSFTYASFHAHKKYGVCLIGTKREDNKSILLNPGPRHIMSATDTCYYINITKEENSAFIFKQEEKHSKGLSAAGVYDAPSRLPVQSIIASMGTVAIDLQNPDPPADSNKLALPTENGAGSRRPSIAPVLEIADSSAILPCDLLNDPSEDETNQSDEDGAPAIDCVKGYPPNSPYIGSSPTLCHLLQQKAPFCCLRLDKGCRHNSFEDAKAYGFKNKLIIVSAETAGNGLYNFIVPLRAYYRPRKELNPIVLLLDNPPDNHFLEAICCFPMVYYMAGTIDNLDNLLQCGIIYADNLVVVDKESTMSAEEDYMADAKTIVNVQTMFRLFPSLSIITELTHPSNMRFMQFRAKDCYSLALSKLEKIERDKGSNLAFMFRLPFAAGRVFSISMLDTLLYQSFVKDYMILIARLLLGLDTTPGSGYLCAMKVTEEDLWIRTYGRLFQKLCSSSAEIPIGIYRTESHMFSSSEVSVEDCEDQRDHRESWKEKTAHRNSTGSDQSEHPLLRKKSMQWARRLSRKNTKPPSRAERINQQRLNLYRRSERQELSELVKNRMKHLGLPTVGYEDVANLTASDVMNRVNLGYLQEGTYMTEGTAVAQTDEMNDNQNTLSYVLINPPPDTILELNDIVYIIRSDPLAHVPENLPGAPAGAKIRQDYRPETRDETHL

Target ORF information:

RefSeq Version	XM_017356387.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio si:dkey-21e5.1 (si:dkey-21e5.1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356387.1

ORF Insert Sequence:

ATGATGGGAC CGCGCAGTCA GTCTCAGGCA GAGCCAAGCA TGACTCCGCC ACGACGCTCT 
TCCACTGCTG CTGACAGGTT GCCTACGGAG ACCGTTCAGA GAAATAACAG CAGTAATACT 
GGTGTTATTC TGGATATTTC CACGCTGAAA TTGGCAGATG TGGACTCTGA GGTCCCTCCT 
CTACCTCCAC GATACCGCTT TCGAGATCTG CTGCTTGGGG ATCAGTCATT TCAGAACGAT 
GACAGGGTGC AGGTGGAGTT TTACGTAAAC GAAAACACAT TTAAGGAGAG GTTGAAGCTC 
TTCTTCATCA AAAACCAAAG GTCAAGTCTC AGAATACGCC TCTTTAACCT CTCCTTAAAG 
TTGTTAACAT GCCTGCTCTA CATAATCAGA GTCTCACTTG ATGATCCAAC TAACCAGGTC 
ACATGTGCAC GGAAACACTG CGCAAACATC ACAGATGCTC TGTGCAGCAA AGCCTGTGCC 
AACTATACAG CAAACCTGAC CGCAGATCCC ACACAAATAA ACTGGGAGTT AATATTTTGG 
GTGAATAGAA GAGTTCCTGT ATGGGCGATA CAGGTGACAG TGGCCTTAAT TAGCTTCTTG 
CAAGCGATGC TCCTGGTGTA TCTCAGCTAC AAGGGCAACA TTTGGGAACA GATTTTCCAA 
ATCTCCTTCA TCATTGAGAT GATCAATACA GTGCCATTCA TAATTACAAT CTTTTGGCCT 
CCGCTTAGGA ACATATTTGT TCCTGTATTT CTTAACTGTT GGCTTGCCAA ATCAGCGCTG 
GAGAATATGA TTAATGATCT CCATCGTGCC ATCCAGCGCA CACACTCTGC CATGTTCAAC 
CAGGTCCTCA TCCTGATCTG TACACTGCTC TGTCTGGTCT TCACCGGGGC TTGTGGGATT 
CAGCACCTGG AGCGAGCAGG GAAGAATCTG ACACTCTTTG ACGCTTTATA TTTCTGCATC 
GTCACCTTCT CCACTGTTGG ATATGGAGAT GTGACGCCCA GAATCTGGCC CTCTCAGCTG 
CTGGTGGTCA TCATGATCTG TGTGGCGCTG GTGGTGCTGC CATTACAGTT CGAGGAGTTG 
GCGTATCTAT GGATGGAGAG GCAGAAGTCA GGTGGAAACT ACAGCAGACA CCGCGCTCAG 
ACAGAGAAAC ACGTGGTGCT CTGCGTCACC TCACTCAAAA TAGACCTGCT CATGGATTTC 
CTCAATGAGT TCTATGCCCA CCCGAGACTA CAGGACTATT ATGTGGTGAT CCTCTGTCCC 
ACGGAGATGG ACATCCAGGT GCGGCGAATA CTGCAGATCC CACTGTGGTC TCAGCGGGTC 
ATCTACCTCC AAGGCTCTGC ACTGAAGGAC CAAGACCTCA TGAGGGCCAA GATGGATGAT 
GCGGAGGCCT GCTTCATCCT CAGCAGCAGA AATGAAGTGG ACCGCACCGC TGCTGATCAT 
CAGACCATTC TGCGAGCCTG GGCTGTGAAG GATTTTGCTC CAAACTGTCC TCTTTATGTC 
CAGATCCTCA AACCCGAGAA CAAATTTCAT GTCAAATTTG CAGATCACGT GGTGTGTGAA 
GAGGAGTTCA AGTACGCCAT GCTGGCCCTG AACTGTGTGT GTCCCGCCAC CTCCACACTG 
GTCACCTTGT TGGTGCACAC GTCTCGAGGA CAAGAAGGTC AGACGTCTCC AGAACAGTGG 
CAGAGGACTT ACGGTCGCTG CTCGGGGAAT GAAGTTTATC ACATCCGTTT GATGGACAGC 
AAGTTTTTTG GAGAATATGA TGGGAAGAGT TTCACTTATG CCTCCTTCCA CGCACACAAG 
AAGTATGGTG TGTGTCTAAT CGGCACAAAG AGAGAAGACA ACAAGAGCAT TCTCCTGAAC 
CCCGGCCCTC GCCACATCAT GTCCGCCACA GACACCTGCT ACTACATCAA CATCACCAAA 
GAGGAGAACT CGGCCTTCAT CTTCAAGCAG GAGGAGAAGC ACAGTAAAGG CTTATCAGCC 
GCCGGCGTGT ACGACGCTCC GTCTCGCCTC CCAGTGCAAA GCATCATTGC CAGCATGGGA 
ACTGTGGCCA TTGACCTGCA GAACCCGGAT CCACCGGCTG ACAGCAATAA ACTGGCTCTG 
CCCACGGAGA ACGGAGCGGG CAGCCGCAGA CCCAGCATTG CCCCTGTGCT GGAGATCGCA 
GACTCCTCTG CCATCCTGCC CTGTGATCTC CTCAACGACC CCTCAGAAGA CGAGACCAAC 
CAATCAGATG AGGATGGAGC GCCGGCCATA GACTGTGTTA AAGGCTATCC TCCAAACTCC 
CCTTACATTG GAAGCTCTCC AACACTTTGC CACCTTCTTC AACAGAAGGC ACCGTTTTGC 
TGTCTACGAC TTGACAAGGG TTGTCGACAC AATAGTTTCG AAGACGCCAA GGCGTATGGT 
TTCAAGAACA AGTTGATTAT CGTTTCTGCT GAGACAGCGG GAAATGGCTT GTACAACTTC 
ATAGTGCCGT TACGTGCCTA TTACCGACCG CGGAAGGAGC TGAATCCCAT TGTGCTGCTG 
CTGGATAATC CGCCTGATAA TCATTTCCTT GAGGCCATCT GCTGTTTCCC TATGGTGTAC 
TACATGGCGG GAACCATAGA CAATTTGGAT AACCTGCTGC AGTGTGGCAT CATCTACGCT 
GATAACCTGG TGGTGGTGGA TAAGGAGAGC ACTATGAGCG CAGAGGAAGA CTACATGGCA 
GACGCTAAAA CCATAGTCAA TGTGCAGACC ATGTTCAGGT TGTTTCCTAG TCTCAGCATA 
ATCACTGAGC TAACTCATCC GTCTAATATG AGGTTCATGC AGTTCCGTGC CAAAGACTGC 
TACTCCCTGG CTCTCTCCAA ACTCGAAAAG ATAGAGCGAG ATAAGGGATC GAATCTGGCG 
TTCATGTTCC GCCTGCCGTT CGCTGCAGGC AGAGTCTTCA GTATCAGCAT GCTGGATACT 
CTGCTTTACC AGTCGTTTGT GAAGGACTAT ATGATTCTCA TTGCGCGGCT CCTCCTGGGT 
TTAGACACCA CCCCGGGCTC AGGATACCTC TGTGCTATGA AGGTAACAGA AGAGGACTTA 
TGGATTCGAA CGTATGGGAG ACTCTTCCAG AAGCTGTGTT CCTCCAGCGC AGAGATTCCC 
ATCGGGATTT ACCGCACCGA ATCCCACATG TTCTCCTCTT CAGAGGTGTC TGTGGAGGAT 
TGCGAGGACC AGCGGGACCA CAGGGAATCC TGGAAAGAGA AAACTGCCCA TAGGAACTCC 
ACAGGGAGCG ACCAATCAGA GCATCCGCTC CTGCGCAAGA AAAGCATGCA GTGGGCGAGA 
CGCCTAAGCA GAAAAAACAC CAAACCTCCC AGTCGAGCTG AACGCATCAA TCAACAGCGC 
CTGAACCTGT ACCGGCGCTC CGAGAGGCAG GAGCTGTCAG AGCTGGTGAA GAACCGCATG 
AAGCATTTAG GCCTGCCCAC TGTGGGATAC GAGGACGTAG CTAATTTAAC CGCAAGCGAT 
GTGATGAACC GAGTAAATCT GGGATATTTG CAAGAGGGTA CTTACATGAC AGAGGGTACT 
GCAGTGGCTC AAACAGATGA AATGAATGAC AATCAGAACA CGTTGTCATA TGTGCTGATA 
AACCCTCCGC CGGACACCAT TCTGGAGCTC AATGACATTG TCTACATCAT CCGTTCTGAT 
CCACTGGCAC ATGTCCCTGA AAATCTCCCA GGGGCTCCAG CTGGAGCGAA GATACGCCAA 
GACTATCGGC CCGAGACAAG GGATGAGACG CATCTGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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si:dkey-21e5.1 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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