C3B.1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	C3B.1
Entrez Gene ID	563665
Full Name	complement component c3b, tandem duplicate 1
Synonyms	si:dkey-21e7.2,wu:fb99g02
General protein information	Preferred Names complement component c3b, tandem duplicate 1 Names complement component c3b, tandem duplicate 1 c3.7 complement component 3-like component c3b, duplicate 1 component c3b, tandem duplicate 1
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001100013.1	NP_001093483.1	complement component c3b, tandem duplicate 1 precursor
XM_009296389.3	XP_009294664.1	complement component c3b, tandem duplicate 1 isoform X1
XM_005174029.4	XP_005174086.1	complement component c3b, tandem duplicate 1 isoform X2
XM_021469338.1	XP_021325013.1	complement component c3b, tandem duplicate 1 isoform X3
XM_009296389.2	XP_009294664.1	complement component c3b, tandem duplicate 1 isoform X1
XM_005174029.3	XP_005174086.1	complement component c3b, tandem duplicate 1 isoform X2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	si:ch211-140f21.1	NP_001093490.1
Danio rerio (zebrafish)	si:dkey-21e7.2	NP_001093483.1

Gene Ontology
Bibliography

Related articles in PubMed

Exposure to mercuric chloride induces developmental damage, oxidative stress and immunotoxicity in zebrafish embryos-larvae.
Zhang QF, Li YW, Liu ZH, Chen QL
Aquatic toxicology (Amsterdam, Netherlands)18176-85(2016 Dec)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

The evolution and appearance of C3 duplications in fish originate an exclusive teleost c3 gene form with anti-inflammatory activity.
Forn-Cun? G, Reis ES, Dios S, Posada D, Lambris JD, Figueras A, Novoa B
PloS one9(6)e99673(2014)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following C3B.1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the C3B.1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa151186	XM_021469338.1 Latest version!	Danio rerio complement component c3b, tandem duplicate 1 (c3b.1), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa35349	NM_001100013.1 Latest version!	Danio rerio complement component c3b, tandem duplicate 1 (c3b.1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa27023	XM_005174029.4 Latest version!	Danio rerio complement component c3b, tandem duplicate 1 (c3b.1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa44129	XM_009296389.3 Latest version!	Danio rerio complement component c3b, tandem duplicate 1 (c3b.1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa44129	XM_009296389.2	Danio rerio complement component c3b, tandem duplicate 1 (c3b.1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa27023	XM_005174029.3	Danio rerio complement component c3b, tandem duplicate 1 (c3b.1), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

C3B.1 ( XM_021469338.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_021325013.1 Danio rerio complement component c3b, tandem duplicate 1 (c3b.1), transcript variant X3, mRNA.

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CloneID	ODa151186
Clone ID Related Accession (Same CDS sequence)	XM_021469338.1
Accession Version	XM_021469338.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5160bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	complement component c3b, tandem duplicate 1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCTTC AGCTGCTGTT ATGGGCGACC CTGCTCTGTC AAATCTGCGA TGGAAAGGCC 
TTCCCTCAAC GACACAAGAG GACGGTTTGG AGACATATGC GACAAATTGA GCCGGTCAAA 
GACCCTTCTC CGTCGTTTTC TCTTCTAGCT CCAGCAAAAA TAAGGCCTGG AACAAAACAG 
AACATCCTGG TGGAGGGACA CAACCTGGCA CAGGTGGTAG AAGTCACTGT TGTGGTTTAC 
GACTATCCAA ACTCCCAAAC TGCCTTGTCA CGGGGCTCAG TTTTCCTCAA TTCAGACAAC 
AACTACAGCG TCCTGCAAAC CCTAGAGATC AACCCAAATT TGCTACTTCC AGATGGAAAA 
AAGAAGTATG TGAAGCTCGT TGCTCAGTTC AGCAACATCT ATCGTGCAGA AAAAGTAATG 
GCGGTGTCGT TCCGCTCCGG ACACATCTTC ATTCAGACCG ACAAACCTAT TTACAACCCT 
GGAGACAAAG TACGTTTCAG GGCTTTTGTG TCTGATTTGG AATTTCGTTC TTCTGAGAGA 
ACCATATCTC TGGAGATTCA GAATCCAGAT GGAATTGCGG TGCATGGTAT TGGGAGAGTT 
AAAGCAATCG ATGGGGTCTT TTCTGACATT TTCTCACTGT CTACTGTAGT CAAAGAGGGC 
AGATGGAAAG TTGTAGCAAA GTTTGATCAG GGAAAAGAGA ATGTTTTCAG CAGAGAGTTT 
GAAGTGAAGA AATATGTGCT TCCAGCATTC AACGTCACAC TTACTCCTAA AACATCCCAC 
CTTAGTCTGG ATGCAGAGAA GTTGGAAGTT GAAATAACAG CTAGGTATCT TTATGGCGCG 
CAAGTGAAGG GTGTTGCGTA TGTGCTGTTT GGTATTGAAA TTAATGGTGA AAAGAAGAGA 
CTCACATCTA TGAAGCAAAT TGATGATCTA AATGGAGGAA GTGTTAGTTT GACTATGGAG 
GAGATAAAGA GAGCTTATCC TGACATAAAC AGTCTTCTGG GGTCTACGTT GTACGTCAAA 
GCATCAGTAT TGACTAGTTC AGGTAGCGAT CTTGTGGAAG CAGAGAAATC CGGCATTAAA 
ATCGTAAAAT CACCCTACAT GCTTACCATT AAGGACACAC CAAAGTATTA TAAACCTGGT 
CTGCCGTTAG CCATGACGGT TATCGTGAGT AACCATGATG GATCTCCAAC TCCTAATATT 
CCTGTTAAAG TCACCTTTCT TGAAAGTCCA ATCAGTGTCC ACAGTGGCAC CATCAACATT 
CACATCAACA TGCCGAAAAC GCACATACTA TATGGCAATT CCCAGATATT GAAAGTGGAG 
ACAGTGGATC CGTCACTGAA ACCAGAACAG CAGGCCATTC AAGAGGTATA CGTCAAGCCA 
TATTCTACTT TTAGCGGTCA CGATAACTTC TTGCACGTCT CAGTGGGAGC AAGTCGAGTG 
GTGGTCGGTG GCAGTGTCAG TGTCCAAATG TACATCAAAT CCTCTTTACC AAACCACAGA 
GCCCTCGTGG AGCATCTCAC ATACATTGTG TTAAATAAGG GGAAAATAAT TCAGGCTGGT 
AGAGTGAATG TCAAAGGCCA GGATGTAACA AGCGTTCATC TGCTGATTAC CACAGAAATG 
CTACCTGCAT TTCGGTTTGT GGCGTACTAC GTTTTGCCAT GGCAGCATAG GGCTGAAGTG 
GTGGCAGACT CCGTGTTTGT TGATGTGGAG GACCGGTGTG TAGGTTCGCT TAATGTTGGA 
CCAGTGGATG GAGAAATAGT CAGTTCTTAC TCACCAGGCA GCAGCTTTAA ATTTCAAGTG 
AAAGGTGATG TTGGAGCAAA GGTCAGCCTG GTAGCTGTGG ATAATGCCAT CTTCTTGTTG 
AGTAAAAACC GCCTGACGCA GAAAAAGGTG TGGGAAGTGG TGGATCAGGC TGATATGGGC 
TGCACCGCAG GAGGAGGCAA GGATAATATG GGTGTTTTCA GTGATGCAGG ACTGATGTTT 
CACTCCAGCA CAGGAGGTTC AACTGACTCC AAAGAAGACT GCTCCAGTAA AACTAAACGG 
CGAAGACGTT CTGCAGCCAA ACTGCAGCTC AGAGATCAGC TCGAGAAGGC CTATTCAGAT 
GAGCTCCTGC AGAGGTGTTG TGTGGATGGC ATGAAGGAGA TTCCCATGCC GTATTCATGC 
TACCGTCGCT CTCTCTACAT CACCGAGGAC TGGAGCTGTG TGTTGGCTTT CCTGCGCTGC 
TGTGCAGAAT ACAGAGGAGA GGAGCTCGGC GTCATTACCA GACCTCCACC AACCACTGCT 
CCACCAACCA TTGCTCCGCC AACCACTTCT CCACCCACCC TCTTTCTCGT ACCACATCTT 
GAATATCATT TTCCGGAACA ACCAGTTTTT TTTGTGAGGG ATAGAGTACT ACAATCCATG 
CCAAAGCTGC ATGCCGAAGC TAATGTCGTC GGAGTTCGTC AGGACAGTTA TCGGGAAGTC 
GTTGTAGAAG AAGAAGAAGA GGCGGCTTCA GTGTTTGAGG AAGATGATTT GTCTGATCTT 
GAGGATATTT ATGTGCGCAG CAAGTTTTTT GAGTCGTGGT TGTGGACCGA TATCAGACTG 
CCTAGCACTG CCAAGTCTGA TGGGTTGGCT GTGTTTCCAG TGGACACCGT TCTTCCAGAC 
AGTATCACAC AGTGGGGGTT TCTAGCTGTC AGTGCCTCAC CTGAGAAAGG TGTCTGCGTA 
GCTGAACCGT ATAACGTCCG CGTGTGGAAG CCGTTCTTTA TCGACCTGCG TTTGCCTCAC 
TCGGTGTCCA GAAATGAACA AGTGGAAATC AAAGCTGTAA TCCACAACTA CAAGAACTCA 
ACACTGGAGG TGATGGTGAT CCTGGAGAAG ACAGAGGACA TGTGTAGTGT GGCCTATACG 
GGACAGCACA GACAGCAGGT TACAGTAGCA GCCGGTTCCT CTAAACTCAT TACATACACC 
ATCATCCCTC TGAAAACAGG GGAGCTCCCT TTACAGGTCA CTGCTGTCGC TGCCTCGTTC 
ATGGGTCAAG ATGCAGTGAA GAAAAATCTA CGCGTAGTAG TTGAAGGCAT TCAGACACTT 
AAAGTGAGGA GCTTTGTGTT AAACCCATCA GAGAAAGGAG GCAGTGCTGG GCAACAGTTG 
ATTCAAGTCA AGAAGATGCA GTTGGATGCT GTTGTGCCAA AATCTGTACC AGAAACATTT 
GTCAATGTCA GAGGCGATTT GCTGGCAGAC AGTATTGATA ACTCCATTAA GGAGGACTCG 
TTGGCAGCAT TGATCCGGAT GCCCGGAGGC TGTGTGGAGC AGAACCTGGC CAGAATCACT 
CTGCCCCTCA TCGCAGCTCA TTACCTGGAC CGCAGTGCAG ACTGGGAGAT CGTGGGAATC 
AGTCGCAGAG AAGAGGCCAT CAAATACATA CAGAGAGGTT ATGAAAATCA ACTTAACTAC 
AAAAAATCAG ACGACTCGTA CCCTCCCTAC GCCAATGAAG GCACCAGCAC ATGGATCACT 
GCGTATGTGG TGAAGGTTTT CTCCATGGCG CATTCATTCA TTAATGTCAA TGAGAAGCAT 
CTGTGTGGCC CTCTGCTCTA TTTACTCAAG AATAAACAGG GTTATGATGG AACTTTTCAA 
GAGGACAATC CCGTCTATGA CACCAGTATG ACGGGAGCTA TGCGGGGATC TGAATACAAA 
GCATCTTTGA CTGCATTTGT GCTCATTGCT TTAGCAGAGG CACAAGATAA AGTTACTTGC 
CAAGATCAAG AAGTCAACAT ACGGGATAAG TCTAGACAGG CAGCAGGGTA TCTGACAGAG 
CATTTCTCCC GGCTGACCAG GCCATATACA GCAGCTATAG CATGCTATGC GCTAGCCGTA 
TCGAACAACG CCTGCGTGAA GAGCATGTTG CTCAAATTTG CCTCACCTGA TCATTCCCAC 
TGGCCTGATT CAAACAATTA TTTTTTCACA CTGGAAGCTA CAGGCTACGC ACTGCTAGCG 
CTGATCAAAA GTGGCCACAT GGAAGAGGCA GAAGCTCCAT TTCGATGGTT GAATGAGCAC 
CGTGGCATTG GTGGAGGATA CGGCTCTACT CAGTCCACCA TGGTGGTTCT TCAGGCCCTG 
TCTGAGTATC TAGTGAAGAG GCCTCCTCCG TCAGACCTCA ATTTGTTGGT TCAGCTCAGC 
GTTCCTGGTC GGAGTGATGT TCGCTGGACC TTCAATCCCA ACTTGGCTTA TGTGGCCCGT 
TCTTCACGAG TCCCTCTTGA TCAGGAATTC TCAGTGGCGG CTTCTGGGAA CGGCAAGGGT 
GTTTTGGAGG TAGTGACGGT TTACCATCAG CTTCCTGATG TGTATGAGAA CAGCACATGC 
AACGGCTTTC AGCTGGACGT CTCTATCGCT GAGACTAATG AAAAAACCTC ATCGGATGTA 
GAAAAGTCCT TCAGACTCAA TGTCAATGTG AGGGCTCTAG AAGAACGGCA AGTGAGGATG 
GTGATTCTAG ACATCGGCCT GCCTACAGGC TTTGAACCCG AAAACTCTGA TTTAGAGTTG 
CTCACAAATT CAGTGGATCG CTACATCAAC AACTTCCAAG TGGTGGACAA CCTGAGCGAC 
AGAGGATCCC TCATAATTCA CCTGTTCAAG GTGTCCAACA AAGAGGCAGA AATGATCTCA 
TTCAGACTCA ATCAGAAATT TAAAGTGGGG CTCCTTCAGC CTTCCACAGT CAAAGTTTAC 
CAGTACTACA ACAAAGATAA AAGCTGTAGC AGGTTCTACT CTCCTCCTGA AGACAAACAG 
CAGCTGGATC AGATCTGTGA AGGCGACGTG TGCCGCTGCT CGCAGGGAGA CTGTTGTGTA 
ATGAAGGAAG ACTTTGCATT TTTTGGTAAA GAGCAGAGGA ATGCCGCTGT ATGCAACGGA 
TTACATCATG GGACGGAGGA AGGTCTGAAT GAGAAGGAGA GGAGGATGTT CCTCTCTCAT 
GCAAGCTGCA GGACCAGGCT TAATTTGAAA GAGGGTCAGG AGTATCTGGT CATTGGGCCG 
ATCACTGATG TTTGGAATGC AGGGAGCACA GTGTCCAACA AGTATGTGTA TACGCTTGGG 
AAAGAAACGT GGGTTGAGCG CTGGCCAGAT GAGTCTGAAT GTGGATCAAC ACTGCAGAAG 
AAATGTGATG AGCTGAAGAG CTTTGCAAAG AAACTGATTG AAGATGGCTG TCAGGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021325013.1
CDS	149..5308
Translation	MLLQLLLWATLLCQICDGKAFPQRHKRTVWRHMRQIEPVKDPSPSFSLLAPAKIRPGTKQNILVEGHNLAQVVEVTVVVYDYPNSQTALSRGSVFLNSDNNYSVLQTLEINPNLLLPDGKKKYVKLVAQFSNIYRAEKVMAVSFRSGHIFIQTDKPIYNPGDKVRFRAFVSDLEFRSSERTISLEIQNPDGIAVHGIGRVKAIDGVFSDIFSLSTVVKEGRWKVVAKFDQGKENVFSREFEVKKYVLPAFNVTLTPKTSHLSLDAEKLEVEITARYLYGAQVKGVAYVLFGIEINGEKKRLTSMKQIDDLNGGSVSLTMEEIKRAYPDINSLLGSTLYVKASVLTSSGSDLVEAEKSGIKIVKSPYMLTIKDTPKYYKPGLPLAMTVIVSNHDGSPTPNIPVKVTFLESPISVHSGTINIHINMPKTHILYGNSQILKVETVDPSLKPEQQAIQEVYVKPYSTFSGHDNFLHVSVGASRVVVGGSVSVQMYIKSSLPNHRALVEHLTYIVLNKGKIIQAGRVNVKGQDVTSVHLLITTEMLPAFRFVAYYVLPWQHRAEVVADSVFVDVEDRCVGSLNVGPVDGEIVSSYSPGSSFKFQVKGDVGAKVSLVAVDNAIFLLSKNRLTQKKVWEVVDQADMGCTAGGGKDNMGVFSDAGLMFHSSTGGSTDSKEDCSSKTKRRRRSAAKLQLRDQLEKAYSDELLQRCCVDGMKEIPMPYSCYRRSLYITEDWSCVLAFLRCCAEYRGEELGVITRPPPTTAPPTIAPPTTSPPTLFLVPHLEYHFPEQPVFFVRDRVLQSMPKLHAEANVVGVRQDSYREVVVEEEEEAASVFEEDDLSDLEDIYVRSKFFESWLWTDIRLPSTAKSDGLAVFPVDTVLPDSITQWGFLAVSASPEKGVCVAEPYNVRVWKPFFIDLRLPHSVSRNEQVEIKAVIHNYKNSTLEVMVILEKTEDMCSVAYTGQHRQQVTVAAGSSKLITYTIIPLKTGELPLQVTAVAASFMGQDAVKKNLRVVVEGIQTLKVRSFVLNPSEKGGSAGQQLIQVKKMQLDAVVPKSVPETFVNVRGDLLADSIDNSIKEDSLAALIRMPGGCVEQNLARITLPLIAAHYLDRSADWEIVGISRREEAIKYIQRGYENQLNYKKSDDSYPPYANEGTSTWITAYVVKVFSMAHSFINVNEKHLCGPLLYLLKNKQGYDGTFQEDNPVYDTSMTGAMRGSEYKASLTAFVLIALAEAQDKVTCQDQEVNIRDKSRQAAGYLTEHFSRLTRPYTAAIACYALAVSNNACVKSMLLKFASPDHSHWPDSNNYFFTLEATGYALLALIKSGHMEEAEAPFRWLNEHRGIGGGYGSTQSTMVVLQALSEYLVKRPPPSDLNLLVQLSVPGRSDVRWTFNPNLAYVARSSRVPLDQEFSVAASGNGKGVLEVVTVYHQLPDVYENSTCNGFQLDVSIAETNEKTSSDVEKSFRLNVNVRALEERQVRMVILDIGLPTGFEPENSDLELLTNSVDRYINNFQVVDNLSDRGSLIIHLFKVSNKEAEMISFRLNQKFKVGLLQPSTVKVYQYYNKDKSCSRFYSPPEDKQQLDQICEGDVCRCSQGDCCVMKEDFAFFGKEQRNAAVCNGLHHGTEEGLNEKERRMFLSHASCRTRLNLKEGQEYLVIGPITDVWNAGSTVSNKYVYTLGKETWVERWPDESECGSTLQKKCDELKSFAKKLIEDGCQA

Target ORF information:

RefSeq Version	XM_021469338.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio complement component c3b, tandem duplicate 1 (c3b.1), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021469338.1

ORF Insert Sequence:

ATGCTTCTTC AGCTGCTGTT ATGGGCGACC CTGCTCTGTC AAATCTGCGA TGGAAAGGCC 
TTCCCTCAAC GACACAAGAG GACGGTTTGG AGACATATGC GACAAATTGA GCCGGTCAAA 
GACCCTTCTC CGTCGTTTTC TCTTCTAGCT CCAGCAAAAA TAAGGCCTGG AACAAAACAG 
AACATCCTGG TGGAGGGACA CAACCTGGCA CAGGTGGTAG AAGTCACTGT TGTGGTTTAC 
GACTATCCAA ACTCCCAAAC TGCCTTGTCA CGGGGCTCAG TTTTCCTCAA TTCAGACAAC 
AACTACAGCG TCCTGCAAAC CCTAGAGATC AACCCAAATT TGCTACTTCC AGATGGAAAA 
AAGAAGTATG TGAAGCTCGT TGCTCAGTTC AGCAACATCT ATCGTGCAGA AAAAGTAATG 
GCGGTGTCGT TCCGCTCCGG ACACATCTTC ATTCAGACCG ACAAACCTAT TTACAACCCT 
GGAGACAAAG TACGTTTCAG GGCTTTTGTG TCTGATTTGG AATTTCGTTC TTCTGAGAGA 
ACCATATCTC TGGAGATTCA GAATCCAGAT GGAATTGCGG TGCATGGTAT TGGGAGAGTT 
AAAGCAATCG ATGGGGTCTT TTCTGACATT TTCTCACTGT CTACTGTAGT CAAAGAGGGC 
AGATGGAAAG TTGTAGCAAA GTTTGATCAG GGAAAAGAGA ATGTTTTCAG CAGAGAGTTT 
GAAGTGAAGA AATATGTGCT TCCAGCATTC AACGTCACAC TTACTCCTAA AACATCCCAC 
CTTAGTCTGG ATGCAGAGAA GTTGGAAGTT GAAATAACAG CTAGGTATCT TTATGGCGCG 
CAAGTGAAGG GTGTTGCGTA TGTGCTGTTT GGTATTGAAA TTAATGGTGA AAAGAAGAGA 
CTCACATCTA TGAAGCAAAT TGATGATCTA AATGGAGGAA GTGTTAGTTT GACTATGGAG 
GAGATAAAGA GAGCTTATCC TGACATAAAC AGTCTTCTGG GGTCTACGTT GTACGTCAAA 
GCATCAGTAT TGACTAGTTC AGGTAGCGAT CTTGTGGAAG CAGAGAAATC CGGCATTAAA 
ATCGTAAAAT CACCCTACAT GCTTACCATT AAGGACACAC CAAAGTATTA TAAACCTGGT 
CTGCCGTTAG CCATGACGGT TATCGTGAGT AACCATGATG GATCTCCAAC TCCTAATATT 
CCTGTTAAAG TCACCTTTCT TGAAAGTCCA ATCAGTGTCC ACAGTGGCAC CATCAACATT 
CACATCAACA TGCCGAAAAC GCACATACTA TATGGCAATT CCCAGATATT GAAAGTGGAG 
ACAGTGGATC CGTCACTGAA ACCAGAACAG CAGGCCATTC AAGAGGTATA CGTCAAGCCA 
TATTCTACTT TTAGCGGTCA CGATAACTTC TTGCACGTCT CAGTGGGAGC AAGTCGAGTG 
GTGGTCGGTG GCAGTGTCAG TGTCCAAATG TACATCAAAT CCTCTTTACC AAACCACAGA 
GCCCTCGTGG AGCATCTCAC ATACATTGTG TTAAATAAGG GGAAAATAAT TCAGGCTGGT 
AGAGTGAATG TCAAAGGCCA GGATGTAACA AGCGTTCATC TGCTGATTAC CACAGAAATG 
CTACCTGCAT TTCGGTTTGT GGCGTACTAC GTTTTGCCAT GGCAGCATAG GGCTGAAGTG 
GTGGCAGACT CCGTGTTTGT TGATGTGGAG GACCGGTGTG TAGGTTCGCT TAATGTTGGA 
CCAGTGGATG GAGAAATAGT CAGTTCTTAC TCACCAGGCA GCAGCTTTAA ATTTCAAGTG 
AAAGGTGATG TTGGAGCAAA GGTCAGCCTG GTAGCTGTGG ATAATGCCAT CTTCTTGTTG 
AGTAAAAACC GCCTGACGCA GAAAAAGGTG TGGGAAGTGG TGGATCAGGC TGATATGGGC 
TGCACCGCAG GAGGAGGCAA GGATAATATG GGTGTTTTCA GTGATGCAGG ACTGATGTTT 
CACTCCAGCA CAGGAGGTTC AACTGACTCC AAAGAAGACT GCTCCAGTAA AACTAAACGG 
CGAAGACGTT CTGCAGCCAA ACTGCAGCTC AGAGATCAGC TCGAGAAGGC CTATTCAGAT 
GAGCTCCTGC AGAGGTGTTG TGTGGATGGC ATGAAGGAGA TTCCCATGCC GTATTCATGC 
TACCGTCGCT CTCTCTACAT CACCGAGGAC TGGAGCTGTG TGTTGGCTTT CCTGCGCTGC 
TGTGCAGAAT ACAGAGGAGA GGAGCTCGGC GTCATTACCA GACCTCCACC AACCACTGCT 
CCACCAACCA TTGCTCCGCC AACCACTTCT CCACCCACCC TCTTTCTCGT ACCACATCTT 
GAATATCATT TTCCGGAACA ACCAGTTTTT TTTGTGAGGG ATAGAGTACT ACAATCCATG 
CCAAAGCTGC ATGCCGAAGC TAATGTCGTC GGAGTTCGTC AGGACAGTTA TCGGGAAGTC 
GTTGTAGAAG AAGAAGAAGA GGCGGCTTCA GTGTTTGAGG AAGATGATTT GTCTGATCTT 
GAGGATATTT ATGTGCGCAG CAAGTTTTTT GAGTCGTGGT TGTGGACCGA TATCAGACTG 
CCTAGCACTG CCAAGTCTGA TGGGTTGGCT GTGTTTCCAG TGGACACCGT TCTTCCAGAC 
AGTATCACAC AGTGGGGGTT TCTAGCTGTC AGTGCCTCAC CTGAGAAAGG TGTCTGCGTA 
GCTGAACCGT ATAACGTCCG CGTGTGGAAG CCGTTCTTTA TCGACCTGCG TTTGCCTCAC 
TCGGTGTCCA GAAATGAACA AGTGGAAATC AAAGCTGTAA TCCACAACTA CAAGAACTCA 
ACACTGGAGG TGATGGTGAT CCTGGAGAAG ACAGAGGACA TGTGTAGTGT GGCCTATACG 
GGACAGCACA GACAGCAGGT TACAGTAGCA GCCGGTTCCT CTAAACTCAT TACATACACC 
ATCATCCCTC TGAAAACAGG GGAGCTCCCT TTACAGGTCA CTGCTGTCGC TGCCTCGTTC 
ATGGGTCAAG ATGCAGTGAA GAAAAATCTA CGCGTAGTAG TTGAAGGCAT TCAGACACTT 
AAAGTGAGGA GCTTTGTGTT AAACCCATCA GAGAAAGGAG GCAGTGCTGG GCAACAGTTG 
ATTCAAGTCA AGAAGATGCA GTTGGATGCT GTTGTGCCAA AATCTGTACC AGAAACATTT 
GTCAATGTCA GAGGCGATTT GCTGGCAGAC AGTATTGATA ACTCCATTAA GGAGGACTCG 
TTGGCAGCAT TGATCCGGAT GCCCGGAGGC TGTGTGGAGC AGAACCTGGC CAGAATCACT 
CTGCCCCTCA TCGCAGCTCA TTACCTGGAC CGCAGTGCAG ACTGGGAGAT CGTGGGAATC 
AGTCGCAGAG AAGAGGCCAT CAAATACATA CAGAGAGGTT ATGAAAATCA ACTTAACTAC 
AAAAAATCAG ACGACTCGTA CCCTCCCTAC GCCAATGAAG GCACCAGCAC ATGGATCACT 
GCGTATGTGG TGAAGGTTTT CTCCATGGCG CATTCATTCA TTAATGTCAA TGAGAAGCAT 
CTGTGTGGCC CTCTGCTCTA TTTACTCAAG AATAAACAGG GTTATGATGG AACTTTTCAA 
GAGGACAATC CCGTCTATGA CACCAGTATG ACGGGAGCTA TGCGGGGATC TGAATACAAA 
GCATCTTTGA CTGCATTTGT GCTCATTGCT TTAGCAGAGG CACAAGATAA AGTTACTTGC 
CAAGATCAAG AAGTCAACAT ACGGGATAAG TCTAGACAGG CAGCAGGGTA TCTGACAGAG 
CATTTCTCCC GGCTGACCAG GCCATATACA GCAGCTATAG CATGCTATGC GCTAGCCGTA 
TCGAACAACG CCTGCGTGAA GAGCATGTTG CTCAAATTTG CCTCACCTGA TCATTCCCAC 
TGGCCTGATT CAAACAATTA TTTTTTCACA CTGGAAGCTA CAGGCTACGC ACTGCTAGCG 
CTGATCAAAA GTGGCCACAT GGAAGAGGCA GAAGCTCCAT TTCGATGGTT GAATGAGCAC 
CGTGGCATTG GTGGAGGATA CGGCTCTACT CAGTCCACCA TGGTGGTTCT TCAGGCCCTG 
TCTGAGTATC TAGTGAAGAG GCCTCCTCCG TCAGACCTCA ATTTGTTGGT TCAGCTCAGC 
GTTCCTGGTC GGAGTGATGT TCGCTGGACC TTCAATCCCA ACTTGGCTTA TGTGGCCCGT 
TCTTCACGAG TCCCTCTTGA TCAGGAATTC TCAGTGGCGG CTTCTGGGAA CGGCAAGGGT 
GTTTTGGAGG TAGTGACGGT TTACCATCAG CTTCCTGATG TGTATGAGAA CAGCACATGC 
AACGGCTTTC AGCTGGACGT CTCTATCGCT GAGACTAATG AAAAAACCTC ATCGGATGTA 
GAAAAGTCCT TCAGACTCAA TGTCAATGTG AGGGCTCTAG AAGAACGGCA AGTGAGGATG 
GTGATTCTAG ACATCGGCCT GCCTACAGGC TTTGAACCCG AAAACTCTGA TTTAGAGTTG 
CTCACAAATT CAGTGGATCG CTACATCAAC AACTTCCAAG TGGTGGACAA CCTGAGCGAC 
AGAGGATCCC TCATAATTCA CCTGTTCAAG GTGTCCAACA AAGAGGCAGA AATGATCTCA 
TTCAGACTCA ATCAGAAATT TAAAGTGGGG CTCCTTCAGC CTTCCACAGT CAAAGTTTAC 
CAGTACTACA ACAAAGATAA AAGCTGTAGC AGGTTCTACT CTCCTCCTGA AGACAAACAG 
CAGCTGGATC AGATCTGTGA AGGCGACGTG TGCCGCTGCT CGCAGGGAGA CTGTTGTGTA 
ATGAAGGAAG ACTTTGCATT TTTTGGTAAA GAGCAGAGGA ATGCCGCTGT ATGCAACGGA 
TTACATCATG GGACGGAGGA AGGTCTGAAT GAGAAGGAGA GGAGGATGTT CCTCTCTCAT 
GCAAGCTGCA GGACCAGGCT TAATTTGAAA GAGGGTCAGG AGTATCTGGT CATTGGGCCG 
ATCACTGATG TTTGGAATGC AGGGAGCACA GTGTCCAACA AGTATGTGTA TACGCTTGGG 
AAAGAAACGT GGGTTGAGCG CTGGCCAGAT GAGTCTGAAT GTGGATCAAC ACTGCAGAAG 
AAATGTGATG AGCTGAAGAG CTTTGCAAAG AAACTGATTG AAGATGGCTG TCAGGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

C3B.1 ( NM_001100013.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001093483.1 Danio rerio complement component c3b, tandem duplicate 1 (c3b.1), mRNA.

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CloneID	ODa35349
Clone ID Related Accession (Same CDS sequence)	NM_001100013.1
Accession Version	NM_001100013.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5043bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Danio rerio(zebrafish)
Product	complement component c3b, tandem duplicate 1 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX890627.4 and BX649330.5. On Jul 26, 2007 this sequence version replaced XM_687024.2. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCTTCTTC AGCTGCTGTT ATGGGCGACC CTGCTCTGTC AAATCTGCGA TGGAAAGGCC 
TTCCCTCAAC GACACAAGAG GACGGTTTGG AGACATATGC GACAAATTGA GCCGGTCAAA 
GACCCTTCTC CGTCGTTTTC TCTTCTAGCT CCAGCAAAAA TAAGGCCTGG AACAAAACAG 
AACATCCTGG TGGAGGGACA CAACCTGGCA CAGGTGGTAG AAGTCACTGT TGTGGTTTAC 
GACTATCCAA ACTCCCAAAC TGCCTTGTCA CGGGGCTCAG TTTTCCTCAA TTCAGACAAC 
AACTACAGCG TCCTGCAAAC CCTAGAGATC AACCCAAATT TGCTACTTCC AGATGGAAAA 
AAGAAGTATG TGAAGCTCGT TGCTCAGTTC AGCAACATCT ATCGTGCAGA AAAAGTAATG 
GCGGTGTCGT TCCGCTCCGG ACACATCTTC ATTCAGACCG ACAAACCTAT TTACAACCCT 
GGAGACAAAG TACGTTTCAG GGCTTTTGTG TCTGATTTGG AATTTCGTTC TTCTGAGAGA 
ACCATATCTC TGGAGATTCA GAATCCAGAT GGAATTGCGG TGCATGGTAT TGGGAGAGTT 
AAAGCAATCG ATGGGGTCTT TTCTGACATT TTCTCACTGT CTACTGTAGT CAAGTATGCA 
CTCAGAATAG TTCAGTTGTG TTTGCTTCCA GCATTCAACG TCACACTTAC TCCTAAAACA 
TCCCACCTTA GTCTGGATGC AGAGAAGTTG GAAGTTGAAA TAACAGCTAG GTATCTTTAT 
GGCGCGCAAG TGAAGGGTGT TGCGTATGTG CTGTTTGGTA TTGAAATTAA TGGTGAAAAG 
AAGAGACTCA CATCTATGAA GCAAATTGAT GATGTACTAA ATGGAGGAAG TGTTAGTTTG 
ACTATGGAGG AGATAAAGAG AGCTTATCCT GACATAAACA GTCTTCTGGG GTCTACGTTG 
TACGTCAAAG CATCAGTATT GACTAGTTCA GGTAGCGATC TTGTGGAAGC AGAGAAATCC 
GGCATTAAAA TCGTAAAATC ACCCTACATG CTTACCATTA AGGACACACC AAAGTATTAT 
AAACCTGGTC TGCCGTTAGC CATGACGGTT ATCGTGAGTA ACCATGATGG ATCTCCAACT 
CCTAATATTC CTGTTAAAGT CACCTTTCTT GAAAGTCCAA TCAGTGTCCA CAGTGGCACC 
ATCAACATTC ACATCAACAT GCCGAAAACG CACATACTAT ATGGCAATTC CCAGATATTG 
AAAGTGGAGA CAGTGGATCC GTCACTGAAA CCAGAACAGC AGGCCATTCA AGAGGTATAC 
GTCAAGCCAT ATTCTACTTT TAGCGGTCAC GATAACTTCT TGCACGTCTC AGTGGGAGCA 
AGTCGAGTGG TGGTCGGTGG CAGTGTCAGT GTCCAAATGT ACATCAAATC CTCTTTACCA 
AACCACAGAG CCCTCGTGGA GCATCTCACA TACATTGTGT TAAATAAGGG GAAAATAATT 
CAGGCTGGTA GAGTGAATGT CAAAGGCCAG GATGTAACAA GCGTTCATCT GCTGATTACC 
ACAGAAATGC TACCTGCATT TCGGTTTGTG GCGTACTACG TTTTGCCATG GCAGCATAGG 
GCTGAAGTGG TGGCAGACTC CGTGTTTGTT GATGTGGAGG ACCGGTGTGT AGGTTCGCTT 
AATGTTGGAC CAGTGGATGG AGAAATAGTC AGTTCTTACT CACCAGGCAG CAGCTTTAAA 
TTTCAAGTGA AAGGTGATGT TGGAGCAAAG GTCAGCCTGG TAGCTGTGGA TAATGCCATC 
TTCTTGTTGA GTAAAAACCG CCTGACGCAG AAAAAGGTGT GGGAAGTGGT GGATCAGGCT 
GATATGGGCT GCACCGCAGG AGGAGGCAAG GATAATATGG GTGTTTTCAG TGATGCAGGA 
CTGATGTTTC ACTCCAGCAC AGGAGGTTCA ACTGACTCCA AAGAAGACTG CTCCAGTAAA 
ACTAAACGGC GAAGACGTTC TGCAGCCAAA CTGCAGCTCA GAGATCAGCT CGAGAAGGCC 
TATTCAGATG AGCTCCTGCA GAGGTGTTGT GTGGATGGCA TGAAGGAGAT TCCCATGCCG 
TATTCATGCT ACCGTCGCTC TCTCTACATC ACCGAGGACT GGAGCTTTTT TTTTGTGAGG 
GATAGAGTAC TACAATCCAT GCCAAAGCTG CATGCCGAAG CTAATGTCGT CGGAGTTCGT 
CAGGACAGTT ATCGGGAAGT CGTTGTAGAA GAAGAAGAAG AGGCGGCTTC AGTGTTTGAG 
GAAGATGATT TGTCTGATCT TGAGGATATT TATGTGCGCA GCAAGTTTTT TGAGTCGTGG 
TTGTGGACCG ATATCAGACT GCCTAGCACT GCCAAGTCTG ATGGGTTGGC TGTGTTTCCA 
GTGGACACCG TTCTTCCAGA CAGTATCACA CAGTGGGGGT TTCTAGCTGT CAGTGCCTCA 
CCTGAGAAAG GTGTCTGCGT AGCTGAACCG TATAACGTCC GCGTGTGGAA GCCGTTCTTT 
ATCGACCTGC GTTTGCCTCA CTCGGTGTCC AGAAATGAAC AAGTGGAAAT CAAAGCTGTA 
ATCCACAACT ACAAGAACTC AACACTGGAG GTACGTTCCA CACCATCAGT AGTGATGGTG 
ATCCTGGAGA AGACAGAGGA CATGTGTAGT GTGGCCTATA CGGGACAGCA CAGACAGCAG 
GTTACAGTAG CAGCCGGTTC CTCTAAACTC ATTACATACA CCATCATCCC TCTGAAAACA 
GGGGAGCTCC CTTTACAGGT CACTGCTGTC GCTGCCTCGT TCATGGGTCA AGATGCAGTG 
AAGAAAAATC TACGCGTAGT AGTTGAAGGC ATTCAGACAC TTAAAGTGAG GAGCTTTGTG 
TTAAACCCAT CAGAGAAAGG AGGCAGTGCT GGGCAACAGT TGATTCAAGT CAAGAAGATG 
CAGTTGGATG CTGTTGTGCC AAAATCTGTA CCAGAAACAT TTGTCAATGT CAGAGGCGAT 
TTGCTGGCAG ACAGTATTGA TAACTCCATT AAGGAGGACT CGTTGGCAGC ATTGATCCGG 
ATGCCCGGAG GCTGTGTGGA GCAGAACCTG GCCAGAATCA CTCTGCCCCT CATCGCAGCT 
CATTACCTGG ACCGCAGTGC AGACTGGGAG ATCGTGGGAA TCAGTCGCAG AGAAGAGGCC 
ATCAAATACA TACAGAGAGG TTATGAAAAT CAACTTAACT ACAAAAAATC AGACGACTCG 
TACCCTCCCT ACGCCAATGA AGGCACCAGC ACATGGATCA CTGCGTATGT GGTGAAGGTT 
TTCTCCATGG CGCATTCATT CATTAATGTC AATGAGAAGC ATCTGTGTGG CCCTCTGCTC 
TATTTACTCA AGAATAAACA GGGTTATGAT GGAACTTTTC AAGAGGACAA TCCCGTCTAT 
GACACCAGTA TGACGGGAGC TATGCGGGGA TCTGAATACA AAGCATCTTT GACTGCATTT 
GTGCTCATTG CTTTAGCAGA GGCACAAGAT AAAGTTACTT GCCAAGATCA AGAAGTCAAC 
ATACGGGATA AGTCTAGACA GGCAGCAGGG TATCTGACAG AGCATTTCTC CCGGCTGACC 
AGGCCATATA CAGCAGCTAT AGCATGCTAT GCGCTAGCCG TATCGAACAA CGCCTGCGTG 
AAGAGCATGT TGCTCAAATT TGCCTCACCT GATCATTCCC ACTGGCCTGA TTCAAACAAT 
TATTTTTTCA CACTGGAAGC TACAGGCTAC GCACTGCTAG CGCTGATCAA AAGTGGCCAC 
ATGGAAGAGG CAGAAGCTCC ATTTCGATGG TTGAATGAGC ACCGTGGCAT TGGTGGAGGA 
TACGGCTCTA CTCAGTCCAC CATGGTGGTT CTTCAGGCCC TGTCTGAGTA TCTAGTGAAG 
AGGCCTCCTC CGTCAGACCT CAATTTGTTG GTTCAGCTCA GCGTTCCTGG TCGGAGTGAT 
GTTCGCTGGA CCTTCAATCC CAACTTGGCT TATGTGGCCC GTTCTTCACG AGTCCCTCTT 
GATCAGGAAT TCTCAGTGGC GGCTTCTGGG AACGGCAAGG GTGTTTTGGA GGTAGTGACG 
GTTTACCATC AGCTTCCTGA TGTGTATGAG AACAGCACAT GCAACGGCTT TCAGCTGGAC 
GTCTCTATCG CTGAGACTAA TGAAAAAACC TCATCGGATG TAGAAAAGTC CTTCAGACTC 
AATGTCAATG TGAGGGCTCT AGAAGAACGG CAAGTGAGGA TGGTGATTCT AGACATCGGC 
CTGCCTACAG GCTTTGAACC CGAAAACTCT GATTTAGAGT TGCTCACAAA TTCAGTGGAT 
CGCTACATCA ACAACTTCCA AGTGGTGGAC AACCTGAGCG ACAGAGGATC CCTCATAATT 
CACCTGTTCA AGGTGTCCAA CAAAGAGGCA GAAATGATCT CATTCAGACT CAATCAGAAA 
TTTAAAGTGG GGCTCCTTCA GCCTTCCACA GTCAAAGTTT ACCAGTACTA CAACAAAGAT 
AAAAGCTGTA GCAGGTTCTA CTCTCCTCCT GAAGACAAAC AGCAGCTGGA TCAGATCTGT 
GAAGGCGACG TGTGCCGCTG CTCGCAGGGA GACTGTTGTG TAATGAAGGA AGACTTTGCA 
TTTTTTGGTA AAGAGCAGAG GAATGCCGCT GTATGCAACG GATTACATCA TGCCTACAGA 
GTCAAGCTGA TCAGTATCAG CCAGAGTCAC TATTACCAGT ATGAGATGGA GATTGTGCAG 
GTTATCAAAG AAGGGACGGA GGAAGGTCTG AATGAGAAGG AGAGGAGGAT GTTCCTCTCT 
CATGCAAGCT GCAGGACCAG GCTTAATTTG AAAGAGGGTC AGGAGTATCT GGTCATTGGG 
CCGATCACTG ATGTTTGGAA TGCAGGGAGC ACAGTGTCCA ACAAGTATGT GTATACGCTT 
GGGAAAGAAA CGTGGGTTGA GCGCTGGCCA GATGAGTCTG AATGTGGATC AACACTGCAG 
AAGAAATGTG ATGAGCTGAA GAGCTTTGCA AAGAAACTGA TTGAAGATGG CTGTCAGGCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001093483.1
CDS	1..5043
Translation	MLLQLLLWATLLCQICDGKAFPQRHKRTVWRHMRQIEPVKDPSPSFSLLAPAKIRPGTKQNILVEGHNLAQVVEVTVVVYDYPNSQTALSRGSVFLNSDNNYSVLQTLEINPNLLLPDGKKKYVKLVAQFSNIYRAEKVMAVSFRSGHIFIQTDKPIYNPGDKVRFRAFVSDLEFRSSERTISLEIQNPDGIAVHGIGRVKAIDGVFSDIFSLSTVVKYALRIVQLCLLPAFNVTLTPKTSHLSLDAEKLEVEITARYLYGAQVKGVAYVLFGIEINGEKKRLTSMKQIDDVLNGGSVSLTMEEIKRAYPDINSLLGSTLYVKASVLTSSGSDLVEAEKSGIKIVKSPYMLTIKDTPKYYKPGLPLAMTVIVSNHDGSPTPNIPVKVTFLESPISVHSGTINIHINMPKTHILYGNSQILKVETVDPSLKPEQQAIQEVYVKPYSTFSGHDNFLHVSVGASRVVVGGSVSVQMYIKSSLPNHRALVEHLTYIVLNKGKIIQAGRVNVKGQDVTSVHLLITTEMLPAFRFVAYYVLPWQHRAEVVADSVFVDVEDRCVGSLNVGPVDGEIVSSYSPGSSFKFQVKGDVGAKVSLVAVDNAIFLLSKNRLTQKKVWEVVDQADMGCTAGGGKDNMGVFSDAGLMFHSSTGGSTDSKEDCSSKTKRRRRSAAKLQLRDQLEKAYSDELLQRCCVDGMKEIPMPYSCYRRSLYITEDWSFFFVRDRVLQSMPKLHAEANVVGVRQDSYREVVVEEEEEAASVFEEDDLSDLEDIYVRSKFFESWLWTDIRLPSTAKSDGLAVFPVDTVLPDSITQWGFLAVSASPEKGVCVAEPYNVRVWKPFFIDLRLPHSVSRNEQVEIKAVIHNYKNSTLEVRSTPSVVMVILEKTEDMCSVAYTGQHRQQVTVAAGSSKLITYTIIPLKTGELPLQVTAVAASFMGQDAVKKNLRVVVEGIQTLKVRSFVLNPSEKGGSAGQQLIQVKKMQLDAVVPKSVPETFVNVRGDLLADSIDNSIKEDSLAALIRMPGGCVEQNLARITLPLIAAHYLDRSADWEIVGISRREEAIKYIQRGYENQLNYKKSDDSYPPYANEGTSTWITAYVVKVFSMAHSFINVNEKHLCGPLLYLLKNKQGYDGTFQEDNPVYDTSMTGAMRGSEYKASLTAFVLIALAEAQDKVTCQDQEVNIRDKSRQAAGYLTEHFSRLTRPYTAAIACYALAVSNNACVKSMLLKFASPDHSHWPDSNNYFFTLEATGYALLALIKSGHMEEAEAPFRWLNEHRGIGGGYGSTQSTMVVLQALSEYLVKRPPPSDLNLLVQLSVPGRSDVRWTFNPNLAYVARSSRVPLDQEFSVAASGNGKGVLEVVTVYHQLPDVYENSTCNGFQLDVSIAETNEKTSSDVEKSFRLNVNVRALEERQVRMVILDIGLPTGFEPENSDLELLTNSVDRYINNFQVVDNLSDRGSLIIHLFKVSNKEAEMISFRLNQKFKVGLLQPSTVKVYQYYNKDKSCSRFYSPPEDKQQLDQICEGDVCRCSQGDCCVMKEDFAFFGKEQRNAAVCNGLHHAYRVKLISISQSHYYQYEMEIVQVIKEGTEEGLNEKERRMFLSHASCRTRLNLKEGQEYLVIGPITDVWNAGSTVSNKYVYTLGKETWVERWPDESECGSTLQKKCDELKSFAKKLIEDGCQA

Target ORF information:

RefSeq Version	NM_001100013.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio complement component c3b, tandem duplicate 1 (c3b.1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001100013.1

ORF Insert Sequence:

ATGCTTCTTC AGCTGCTGTT ATGGGCGACC CTGCTCTGTC AAATCTGCGA TGGAAAGGCC 
TTCCCTCAAC GACACAAGAG GACGGTTTGG AGACATATGC GACAAATTGA GCCGGTCAAA 
GACCCTTCTC CGTCGTTTTC TCTTCTAGCT CCAGCAAAAA TAAGGCCTGG AACAAAACAG 
AACATCCTGG TGGAGGGACA CAACCTGGCA CAGGTGGTAG AAGTCACTGT TGTGGTTTAC 
GACTATCCAA ACTCCCAAAC TGCCTTGTCA CGGGGCTCAG TTTTCCTCAA TTCAGACAAC 
AACTACAGCG TCCTGCAAAC CCTAGAGATC AACCCAAATT TGCTACTTCC AGATGGAAAA 
AAGAAGTATG TGAAGCTCGT TGCTCAGTTC AGCAACATCT ATCGTGCAGA AAAAGTAATG 
GCGGTGTCGT TCCGCTCCGG ACACATCTTC ATTCAGACCG ACAAACCTAT TTACAACCCT 
GGAGACAAAG TACGTTTCAG GGCTTTTGTG TCTGATTTGG AATTTCGTTC TTCTGAGAGA 
ACCATATCTC TGGAGATTCA GAATCCAGAT GGAATTGCGG TGCATGGTAT TGGGAGAGTT 
AAAGCAATCG ATGGGGTCTT TTCTGACATT TTCTCACTGT CTACTGTAGT CAAGTATGCA 
CTCAGAATAG TTCAGTTGTG TTTGCTTCCA GCATTCAACG TCACACTTAC TCCTAAAACA 
TCCCACCTTA GTCTGGATGC AGAGAAGTTG GAAGTTGAAA TAACAGCTAG GTATCTTTAT 
GGCGCGCAAG TGAAGGGTGT TGCGTATGTG CTGTTTGGTA TTGAAATTAA TGGTGAAAAG 
AAGAGACTCA CATCTATGAA GCAAATTGAT GATGTACTAA ATGGAGGAAG TGTTAGTTTG 
ACTATGGAGG AGATAAAGAG AGCTTATCCT GACATAAACA GTCTTCTGGG GTCTACGTTG 
TACGTCAAAG CATCAGTATT GACTAGTTCA GGTAGCGATC TTGTGGAAGC AGAGAAATCC 
GGCATTAAAA TCGTAAAATC ACCCTACATG CTTACCATTA AGGACACACC AAAGTATTAT 
AAACCTGGTC TGCCGTTAGC CATGACGGTT ATCGTGAGTA ACCATGATGG ATCTCCAACT 
CCTAATATTC CTGTTAAAGT CACCTTTCTT GAAAGTCCAA TCAGTGTCCA CAGTGGCACC 
ATCAACATTC ACATCAACAT GCCGAAAACG CACATACTAT ATGGCAATTC CCAGATATTG 
AAAGTGGAGA CAGTGGATCC GTCACTGAAA CCAGAACAGC AGGCCATTCA AGAGGTATAC 
GTCAAGCCAT ATTCTACTTT TAGCGGTCAC GATAACTTCT TGCACGTCTC AGTGGGAGCA 
AGTCGAGTGG TGGTCGGTGG CAGTGTCAGT GTCCAAATGT ACATCAAATC CTCTTTACCA 
AACCACAGAG CCCTCGTGGA GCATCTCACA TACATTGTGT TAAATAAGGG GAAAATAATT 
CAGGCTGGTA GAGTGAATGT CAAAGGCCAG GATGTAACAA GCGTTCATCT GCTGATTACC 
ACAGAAATGC TACCTGCATT TCGGTTTGTG GCGTACTACG TTTTGCCATG GCAGCATAGG 
GCTGAAGTGG TGGCAGACTC CGTGTTTGTT GATGTGGAGG ACCGGTGTGT AGGTTCGCTT 
AATGTTGGAC CAGTGGATGG AGAAATAGTC AGTTCTTACT CACCAGGCAG CAGCTTTAAA 
TTTCAAGTGA AAGGTGATGT TGGAGCAAAG GTCAGCCTGG TAGCTGTGGA TAATGCCATC 
TTCTTGTTGA GTAAAAACCG CCTGACGCAG AAAAAGGTGT GGGAAGTGGT GGATCAGGCT 
GATATGGGCT GCACCGCAGG AGGAGGCAAG GATAATATGG GTGTTTTCAG TGATGCAGGA 
CTGATGTTTC ACTCCAGCAC AGGAGGTTCA ACTGACTCCA AAGAAGACTG CTCCAGTAAA 
ACTAAACGGC GAAGACGTTC TGCAGCCAAA CTGCAGCTCA GAGATCAGCT CGAGAAGGCC 
TATTCAGATG AGCTCCTGCA GAGGTGTTGT GTGGATGGCA TGAAGGAGAT TCCCATGCCG 
TATTCATGCT ACCGTCGCTC TCTCTACATC ACCGAGGACT GGAGCTTTTT TTTTGTGAGG 
GATAGAGTAC TACAATCCAT GCCAAAGCTG CATGCCGAAG CTAATGTCGT CGGAGTTCGT 
CAGGACAGTT ATCGGGAAGT CGTTGTAGAA GAAGAAGAAG AGGCGGCTTC AGTGTTTGAG 
GAAGATGATT TGTCTGATCT TGAGGATATT TATGTGCGCA GCAAGTTTTT TGAGTCGTGG 
TTGTGGACCG ATATCAGACT GCCTAGCACT GCCAAGTCTG ATGGGTTGGC TGTGTTTCCA 
GTGGACACCG TTCTTCCAGA CAGTATCACA CAGTGGGGGT TTCTAGCTGT CAGTGCCTCA 
CCTGAGAAAG GTGTCTGCGT AGCTGAACCG TATAACGTCC GCGTGTGGAA GCCGTTCTTT 
ATCGACCTGC GTTTGCCTCA CTCGGTGTCC AGAAATGAAC AAGTGGAAAT CAAAGCTGTA 
ATCCACAACT ACAAGAACTC AACACTGGAG GTACGTTCCA CACCATCAGT AGTGATGGTG 
ATCCTGGAGA AGACAGAGGA CATGTGTAGT GTGGCCTATA CGGGACAGCA CAGACAGCAG 
GTTACAGTAG CAGCCGGTTC CTCTAAACTC ATTACATACA CCATCATCCC TCTGAAAACA 
GGGGAGCTCC CTTTACAGGT CACTGCTGTC GCTGCCTCGT TCATGGGTCA AGATGCAGTG 
AAGAAAAATC TACGCGTAGT AGTTGAAGGC ATTCAGACAC TTAAAGTGAG GAGCTTTGTG 
TTAAACCCAT CAGAGAAAGG AGGCAGTGCT GGGCAACAGT TGATTCAAGT CAAGAAGATG 
CAGTTGGATG CTGTTGTGCC AAAATCTGTA CCAGAAACAT TTGTCAATGT CAGAGGCGAT 
TTGCTGGCAG ACAGTATTGA TAACTCCATT AAGGAGGACT CGTTGGCAGC ATTGATCCGG 
ATGCCCGGAG GCTGTGTGGA GCAGAACCTG GCCAGAATCA CTCTGCCCCT CATCGCAGCT 
CATTACCTGG ACCGCAGTGC AGACTGGGAG ATCGTGGGAA TCAGTCGCAG AGAAGAGGCC 
ATCAAATACA TACAGAGAGG TTATGAAAAT CAACTTAACT ACAAAAAATC AGACGACTCG 
TACCCTCCCT ACGCCAATGA AGGCACCAGC ACATGGATCA CTGCGTATGT GGTGAAGGTT 
TTCTCCATGG CGCATTCATT CATTAATGTC AATGAGAAGC ATCTGTGTGG CCCTCTGCTC 
TATTTACTCA AGAATAAACA GGGTTATGAT GGAACTTTTC AAGAGGACAA TCCCGTCTAT 
GACACCAGTA TGACGGGAGC TATGCGGGGA TCTGAATACA AAGCATCTTT GACTGCATTT 
GTGCTCATTG CTTTAGCAGA GGCACAAGAT AAAGTTACTT GCCAAGATCA AGAAGTCAAC 
ATACGGGATA AGTCTAGACA GGCAGCAGGG TATCTGACAG AGCATTTCTC CCGGCTGACC 
AGGCCATATA CAGCAGCTAT AGCATGCTAT GCGCTAGCCG TATCGAACAA CGCCTGCGTG 
AAGAGCATGT TGCTCAAATT TGCCTCACCT GATCATTCCC ACTGGCCTGA TTCAAACAAT 
TATTTTTTCA CACTGGAAGC TACAGGCTAC GCACTGCTAG CGCTGATCAA AAGTGGCCAC 
ATGGAAGAGG CAGAAGCTCC ATTTCGATGG TTGAATGAGC ACCGTGGCAT TGGTGGAGGA 
TACGGCTCTA CTCAGTCCAC CATGGTGGTT CTTCAGGCCC TGTCTGAGTA TCTAGTGAAG 
AGGCCTCCTC CGTCAGACCT CAATTTGTTG GTTCAGCTCA GCGTTCCTGG TCGGAGTGAT 
GTTCGCTGGA CCTTCAATCC CAACTTGGCT TATGTGGCCC GTTCTTCACG AGTCCCTCTT 
GATCAGGAAT TCTCAGTGGC GGCTTCTGGG AACGGCAAGG GTGTTTTGGA GGTAGTGACG 
GTTTACCATC AGCTTCCTGA TGTGTATGAG AACAGCACAT GCAACGGCTT TCAGCTGGAC 
GTCTCTATCG CTGAGACTAA TGAAAAAACC TCATCGGATG TAGAAAAGTC CTTCAGACTC 
AATGTCAATG TGAGGGCTCT AGAAGAACGG CAAGTGAGGA TGGTGATTCT AGACATCGGC 
CTGCCTACAG GCTTTGAACC CGAAAACTCT GATTTAGAGT TGCTCACAAA TTCAGTGGAT 
CGCTACATCA ACAACTTCCA AGTGGTGGAC AACCTGAGCG ACAGAGGATC CCTCATAATT 
CACCTGTTCA AGGTGTCCAA CAAAGAGGCA GAAATGATCT CATTCAGACT CAATCAGAAA 
TTTAAAGTGG GGCTCCTTCA GCCTTCCACA GTCAAAGTTT ACCAGTACTA CAACAAAGAT 
AAAAGCTGTA GCAGGTTCTA CTCTCCTCCT GAAGACAAAC AGCAGCTGGA TCAGATCTGT 
GAAGGCGACG TGTGCCGCTG CTCGCAGGGA GACTGTTGTG TAATGAAGGA AGACTTTGCA 
TTTTTTGGTA AAGAGCAGAG GAATGCCGCT GTATGCAACG GATTACATCA TGCCTACAGA 
GTCAAGCTGA TCAGTATCAG CCAGAGTCAC TATTACCAGT ATGAGATGGA GATTGTGCAG 
GTTATCAAAG AAGGGACGGA GGAAGGTCTG AATGAGAAGG AGAGGAGGAT GTTCCTCTCT 
CATGCAAGCT GCAGGACCAG GCTTAATTTG AAAGAGGGTC AGGAGTATCT GGTCATTGGG 
CCGATCACTG ATGTTTGGAA TGCAGGGAGC ACAGTGTCCA ACAAGTATGT GTATACGCTT 
GGGAAAGAAA CGTGGGTTGA GCGCTGGCCA GATGAGTCTG AATGTGGATC AACACTGCAG 
AAGAAATGTG ATGAGCTGAA GAGCTTTGCA AAGAAACTGA TTGAAGATGG CTGTCAGGCT 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

C3B.1 ( XM_005174029.4 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005174086.1 Danio rerio complement component c3b, tandem duplicate 1 (c3b.1), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa27023
Clone ID Related Accession (Same CDS sequence)	XM_005174029.4 , XM_005174029.3
Accession Version	XM_005174029.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	complement component c3b, tandem duplicate 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005174029.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCTTC AGCTGCTGTT ATGGGCGACC CTGCTCTGTC AAATCTGCGA TGGAAAGGCC 
TTCCCTCAAC GACACAAGAG GACGGTTTGG AGACATATGC GACAAATTGA GCCGGTCAAA 
GACCCTTCTC CGTCGTTTTC TCTTCTAGCT CCAGCAAAAA TAAGGCCTGG AACAAAACAG 
AACATCCTGG TGGAGGGACA CAACCTGGCA CAGGTGGTAG AAGTCACTGT TGTGGTTTAC 
GACTATCCAA ACTCCCAAAC TGCCTTGTCA CGGGGCTCAG TTTTCCTCAA TTCAGACAAC 
AACTACAGCG TCCTGCAAAC CCTAGAGATC AACCCAAATT TGCTACTTCC AGATGGAAAA 
AAGAAGTATG TGAAGCTCGT TGCTCAGTTC AGCAACATCT ATCGTGCAGA AAAAGTAATG 
GCGGTGTCGT TCCGCTCCGG ACACATCTTC ATTCAGACCG ACAAACCTAT TTACAACCCT 
GGAGACAAAG TACGTTTCAG GGCTTTTGTG TCTGATTTGG AATTTCGTTC TTCTGAGAGA 
ACCATATCTC TGGAGATTCA GAATCCAGAT GGAATTGCGG TGCATGGTAT TGGGAGAGTT 
AAAGCAATCG ATGGGGTCTT TTCTGACATT TTCTCACTGT CTACTGTAGT CAAAGAGGGC 
AGATGGAAAG TTGTAGCAAA GTTTGATCAG GGAAAAGAGA ATGTTTTCAG CAGAGAGTTT 
GAAGTGAAGA AATATGTGCT TCCAGCATTC AACGTCACAC TTACTCCTAA AACATCCCAC 
CTTAGTCTGG ATGCAGAGAA GTTGGAAGTT GAAATAACAG CTAGGTATCT TTATGGCGCG 
CAAGTGAAGG GTGTTGCGTA TGTGCTGTTT GGTATTGAAA TTAATGGTGA AAAGAAGAGA 
CTCACATCTA TGAAGCAAAT TGATGATCTA AATGGAGGAA GTGTTAGTTT GACTATGGAG 
GAGATAAAGA GAGCTTATCC TGACATAAAC AGTCTTCTGG GGTCTACGTT GTACGTCAAA 
GCATCAGTAT TGACTAGTTC AGGTAGCGAT CTTGTGGAAG CAGAGAAATC CGGCATTAAA 
ATCGTAAAAT CACCCTACAT GCTTACCATT AAGGACACAC CAAAGTATTA TAAACCTGGT 
CTGCCGTTAG CCATGACGGT TATCGTGAGT AACCATGATG GATCTCCAAC TCCTAATATT 
CCTGTTAAAG TCACCTTTCT TGAAAGTCCA ATCAGTGTCC ACAGTGGCAC CATCAACATT 
CACATCAACA TGCCGAAAAC GCACATACTA TATGGCAATT CCCAGATATT GAAAGTGGAG 
ACAGTGGATC CGTCACTGAA ACCAGAACAG CAGGCCATTC AAGAGGTATA CGTCAAGCCA 
TATTCTACTT TTAGCGGTCA CGATAACTTC TTGCACGTCT CAGTGGGAGC AAGTCGAGTG 
GTGGTCGGTG GCAGTGTCAG TGTCCAAATG TACATCAAAT CCTCTTTACC AAACCACAGA 
GCCCTCGTGG AGCATCTCAC ATACATTGTG TTAAATAAGG GGAAAATAAT TCAGGCTGGT 
AGAGTGAATG TCAAAGGCCA GGATGTAACA AGCGTTCATC TGCTGATTAC CACAGAAATG 
CTACCTGCAT TTCGGTTTGT GGCGTACTAC GTTTTGCCAT GGCAGCATAG GGCTGAAGTG 
GTGGCAGACT CCGTGTTTGT TGATGTGGAG GACCGGTGTG TAGGTTCGCT TAATGTTGGA 
CCAGTGGATG GAGAAATAGT CAGTTCTTAC TCACCAGGCA GCAGCTTTAA ATTTCAAGTG 
AAAGGTGATG TTGGAGCAAA GGTCAGCCTG GTAGCTGTGG ATAATGCCAT CTTCTTGTTG 
AGTAAAAACC GCCTGACGCA GAAAAAGGTG TGGGAAGTGG TGGATCAGGC TGATATGGGC 
TGCACCGCAG GAGGAGGCAA GGATAATATG GGTGTTTTCA GTGATGCAGG ACTGATGTTT 
CACTCCAGCA CAGGAGGTTC AACTGACTCC AAAGAAGACT GCTCCAGTAA AACTAAACGG 
CGAAGACGTT CTGCAGCCAA ACTGCAGCTC AGAGATCAGC TCGAGAAGGC CTATTCAGAT 
GAGCTCCTGC AGAGGTGTTG TGTGGATGGC ATGAAGGAGA TTCCCATGCC GTATTCATGC 
TACCGTCGCT CTCTCTACAT CACCGAGGAC TGGAGCTGTG TGTTGGCTTT CCTGCGCTGC 
TGTGCAGAAT ACAGAGGAGA GGAGCTCGGC GTCATTACCA GACCTCCACC AACCACTGCT 
CCACCAACCA TTGCTCCGCC AACCACTTCT CCACCCACCC TCTTTCTCGT ACCACATCTT 
GAATATCATT TTCCGGAACA ACCAGTTTTT TTTGTGAGGG ATAGAGTACT ACAATCCATG 
CCAAAGCTGC ATGCCGAAGC TAATGTCGTC GGAGTTCGTC AGGACAGTTA TCGGGAAGTC 
GTTGTAGAAG AAGAAGAAGA GGCGGCTTCA GTGTTTGAGG AAGATGATTT GTCTGATCTT 
GAGGATATTT ATGTGCGCAG CAAGTTTTTT GAGTCGTGGT TGTGGACCGA TATCAGACTG 
CCTAGCACTG CCAAGTCTGA TGGGTTGGCT GTGTTTCCAG TGGACACCGT TCTTCCAGAC 
AGTATCACAC AGTGGGGGTT TCTAGCTGTC AGTGCCTCAC CTGAGAAAGG TGTCTGCGTA 
GCTGAACCGT ATAACGTCCG CGTGTGGAAG CCGTTCTTTA TCGACCTGCG TTTGCCTCAC 
TCGGTGTCCA GAAATGAACA AGTGGAAATC AAAGCTGTAA TCCACAACTA CAAGAACTCA 
ACACTGGAGG TGATGGTGAT CCTGGAGAAG ACAGAGGACA TGTGTAGTGT GGCCTATACG 
GGACAGCACA GACAGCAGGT TACAGTAGCA GCCGGTTCCT CTAAACTCAT TACATACACC 
ATCATCCCTC TGAAAACAGG GGAGCTCCCT TTACAGGTCA CTGCTGTCGC TGCCTCGTTC 
ATGGGTCAAG ATGCAGTGAA GAAAAATCTA CGCGTAGTAG TTGAAGGCAT TCAGACACTT 
AAAGTGAGGA GCTTTGTGTT AAACCCATCA GAGAAAGGAG GCAGTGCTGG GCAACAGTTG 
ATTCAAGTCA AGAAGATGCA GTTGGATGCT GTTGTGCCAA AATCTGTACC AGAAACATTT 
GTCAATGTCA GAGGCGATTT GCTGGCAGAC AGTATTGATA ACTCCATTAA GGAGGACTCG 
TTGGCAGCAT TGATCCGGAT GCCCGGAGGC TGTGTGGAGC AGAACCTGGC CAGAATCACT 
CTGCCCCTCA TCGCAGCTCA TTACCTGGAC CGCAGTGCAG ACTGGGAGAT CGTGGGAATC 
AGTCGCAGAG AAGAGGCCAT CAAATACATA CAGAGAGGTT ATGAAAATCA ACTTAACTAC 
AAAAAATCAG ACGACTCGTA CCCTCCCTAC GCCAATGAAG GCACCAGCAC ATGGATCACT 
GCGTATGTGG TGAAGGTTTT CTCCATGGCG CATTCATTCA TTAATGTCAA TGAGAAGCAT 
CTGTGTGGCC CTCTGCTCTA TTTACTCAAG AATAAACAGG GTTATGATGG AACTTTTCAA 
GAGGACAATC CCGTCTATGA CACCAGTATG ACGGGAGCTA TGCGGGGATC TGAATACAAA 
GCATCTTTGA CTGCATTTGT GCTCATTGCT TTAGCAGAGG CACAAGATAA AGTTACTTGC 
CAAGATCAAG AAGTCAACAT ACGGGATAAG TCTAGACAGG CAGCAGGGTA TCTGACAGAG 
CATTTCTCCC GGCTGACCAG GCCATATACA GCAGCTATAG CATGCTATGC GCTAGCCGTA 
TCGAACAACG CCTGCGTGAA GAGCATGTTG CTCAAATTTG CCTCACCTGA TCATTCCCAC 
TGGCCTGATT CAAACAATTA TTTTTTCACA CTGGAAGCTA CAGGCTACGC ACTGCTAGCG 
CTGATCAAAA GTGGCCACAT GGAAGAGGCA GAAGCTCCAT TTCGATGGTT GAATGAGCAC 
CGTGGCATTG GTGGAGGATA CGGCTCTACT CAGTCCACCA TGGTGGTTCT TCAGGCCCTG 
TCTGAGTATC TAGTGAAGAG GCCTCCTCCG TCAGACCTCA ATTTGTTGGT TCAGCTCAGC 
GTTCCTGGTC GGAGTGATGT TCGCTGGACC TTCAATCCCA ACTTGGCTTA TGTGGCCCGT 
TCTTCACGAG TCCCTCTTGA TCAGGAATTC TCAGTGGCGG CTTCTGGGAA CGGCAAGGGT 
GTTTTGGAGG TAGTGACGGT TTACCATCAG CTTCCTGATG TGTATGAGAA CAGCACATGC 
AACGGCTTTC AGCTGGACGT CTCTATCGCT GAGACTAATG AAAAAACCTC ATCGGATGTA 
GAAAAGTCCT TCAGACTCAA TGTCAATGTG AGGGCTCTAG AAGAACGGCA AGTGAGGATG 
GTGATTCTAG ACATCGGCCT GCCTACAGGC TTTGAACCCG AAAACTCTGA TTTAGAGTTG 
CTCACAAATT CAGTGGATCG CTACATCAAC AACTTCCAAG TGGTGGACAA CCTGAGCGAC 
AGAGGATCCC TCATAATTCA CCTGTTCAAG GTGTCCAACA AAGAGGCAGA AATGATCTCA 
TTCAGACTCA ATCAGAAATT TAAAGTGGGG CTCCTTCAGC CTTCCACAGT CAAAGTTTAC 
CAGTACTACA ACAAAGATAA AAGCTGTAGC AGGTTCTACT CTCCTCCTGA AGACAAACAG 
CAGCTGGATC AGATCTGTGA AGGCGACGTG TGCCGCTGCT CGCAGGGAGA CTGTTGTGTA 
ATGAAGGAAG ACTTTGCATT TTTTGGTAAA GAGCAGAGGA ATGCCGCTGT ATGCAACGGA 
TTACATCATG CCTACAGAGT CAAGCTGATC AGTATCAGCC AGAGTCACTA TTACCAGTAT 
GAGATGGAGA TTGTGCAGGT TATCAAAGAA GGGACGGAGG AAGGTCTGAA TGAGAAGGAG 
AGGAGGATGT TCCTCTCTCA TGCAAGCTGC AGGACCAGGC TTAATTTGAA AGAGGGTCAG 
GAGTATCTGG TCATTGGGCC GATCACTGAT GTTTGGAATG CAGGGAGCAC AGTGTCCAAC 
AAGTATGTGT ATACGCTTGG GAAAGAAACG TGGGTTGAGC GCTGGCCAGA TGAGTCTGAA 
TGTGGATCAA CACTGCAGAA GAAATGTGAT GAGCTGAAGA GCTTTGCAAA GAAACTGATT 
GAAGATGGCT GTCAGGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005174086.1
CDS	149..5389
Translation	MLLQLLLWATLLCQICDGKAFPQRHKRTVWRHMRQIEPVKDPSPSFSLLAPAKIRPGTKQNILVEGHNLAQVVEVTVVVYDYPNSQTALSRGSVFLNSDNNYSVLQTLEINPNLLLPDGKKKYVKLVAQFSNIYRAEKVMAVSFRSGHIFIQTDKPIYNPGDKVRFRAFVSDLEFRSSERTISLEIQNPDGIAVHGIGRVKAIDGVFSDIFSLSTVVKEGRWKVVAKFDQGKENVFSREFEVKKYVLPAFNVTLTPKTSHLSLDAEKLEVEITARYLYGAQVKGVAYVLFGIEINGEKKRLTSMKQIDDLNGGSVSLTMEEIKRAYPDINSLLGSTLYVKASVLTSSGSDLVEAEKSGIKIVKSPYMLTIKDTPKYYKPGLPLAMTVIVSNHDGSPTPNIPVKVTFLESPISVHSGTINIHINMPKTHILYGNSQILKVETVDPSLKPEQQAIQEVYVKPYSTFSGHDNFLHVSVGASRVVVGGSVSVQMYIKSSLPNHRALVEHLTYIVLNKGKIIQAGRVNVKGQDVTSVHLLITTEMLPAFRFVAYYVLPWQHRAEVVADSVFVDVEDRCVGSLNVGPVDGEIVSSYSPGSSFKFQVKGDVGAKVSLVAVDNAIFLLSKNRLTQKKVWEVVDQADMGCTAGGGKDNMGVFSDAGLMFHSSTGGSTDSKEDCSSKTKRRRRSAAKLQLRDQLEKAYSDELLQRCCVDGMKEIPMPYSCYRRSLYITEDWSCVLAFLRCCAEYRGEELGVITRPPPTTAPPTIAPPTTSPPTLFLVPHLEYHFPEQPVFFVRDRVLQSMPKLHAEANVVGVRQDSYREVVVEEEEEAASVFEEDDLSDLEDIYVRSKFFESWLWTDIRLPSTAKSDGLAVFPVDTVLPDSITQWGFLAVSASPEKGVCVAEPYNVRVWKPFFIDLRLPHSVSRNEQVEIKAVIHNYKNSTLEVMVILEKTEDMCSVAYTGQHRQQVTVAAGSSKLITYTIIPLKTGELPLQVTAVAASFMGQDAVKKNLRVVVEGIQTLKVRSFVLNPSEKGGSAGQQLIQVKKMQLDAVVPKSVPETFVNVRGDLLADSIDNSIKEDSLAALIRMPGGCVEQNLARITLPLIAAHYLDRSADWEIVGISRREEAIKYIQRGYENQLNYKKSDDSYPPYANEGTSTWITAYVVKVFSMAHSFINVNEKHLCGPLLYLLKNKQGYDGTFQEDNPVYDTSMTGAMRGSEYKASLTAFVLIALAEAQDKVTCQDQEVNIRDKSRQAAGYLTEHFSRLTRPYTAAIACYALAVSNNACVKSMLLKFASPDHSHWPDSNNYFFTLEATGYALLALIKSGHMEEAEAPFRWLNEHRGIGGGYGSTQSTMVVLQALSEYLVKRPPPSDLNLLVQLSVPGRSDVRWTFNPNLAYVARSSRVPLDQEFSVAASGNGKGVLEVVTVYHQLPDVYENSTCNGFQLDVSIAETNEKTSSDVEKSFRLNVNVRALEERQVRMVILDIGLPTGFEPENSDLELLTNSVDRYINNFQVVDNLSDRGSLIIHLFKVSNKEAEMISFRLNQKFKVGLLQPSTVKVYQYYNKDKSCSRFYSPPEDKQQLDQICEGDVCRCSQGDCCVMKEDFAFFGKEQRNAAVCNGLHHAYRVKLISISQSHYYQYEMEIVQVIKEGTEEGLNEKERRMFLSHASCRTRLNLKEGQEYLVIGPITDVWNAGSTVSNKYVYTLGKETWVERWPDESECGSTLQKKCDELKSFAKKLIEDGCQA

Target ORF information:

RefSeq Version	XM_005174029.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio complement component c3b, tandem duplicate 1 (c3b.1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005174029.4

ORF Insert Sequence:

ATGCTTCTTC AGCTGCTGTT ATGGGCGACC CTGCTCTGTC AAATCTGCGA TGGAAAGGCC 
TTCCCTCAAC GACACAAGAG GACGGTTTGG AGACATATGC GACAAATTGA GCCGGTCAAA 
GACCCTTCTC CGTCGTTTTC TCTTCTAGCT CCAGCAAAAA TAAGGCCTGG AACAAAACAG 
AACATCCTGG TGGAGGGACA CAACCTGGCA CAGGTGGTAG AAGTCACTGT TGTGGTTTAC 
GACTATCCAA ACTCCCAAAC TGCCTTGTCA CGGGGCTCAG TTTTCCTCAA TTCAGACAAC 
AACTACAGCG TCCTGCAAAC CCTAGAGATC AACCCAAATT TGCTACTTCC AGATGGAAAA 
AAGAAGTATG TGAAGCTCGT TGCTCAGTTC AGCAACATCT ATCGTGCAGA AAAAGTAATG 
GCGGTGTCGT TCCGCTCCGG ACACATCTTC ATTCAGACCG ACAAACCTAT TTACAACCCT 
GGAGACAAAG TACGTTTCAG GGCTTTTGTG TCTGATTTGG AATTTCGTTC TTCTGAGAGA 
ACCATATCTC TGGAGATTCA GAATCCAGAT GGAATTGCGG TGCATGGTAT TGGGAGAGTT 
AAAGCAATCG ATGGGGTCTT TTCTGACATT TTCTCACTGT CTACTGTAGT CAAAGAGGGC 
AGATGGAAAG TTGTAGCAAA GTTTGATCAG GGAAAAGAGA ATGTTTTCAG CAGAGAGTTT 
GAAGTGAAGA AATATGTGCT TCCAGCATTC AACGTCACAC TTACTCCTAA AACATCCCAC 
CTTAGTCTGG ATGCAGAGAA GTTGGAAGTT GAAATAACAG CTAGGTATCT TTATGGCGCG 
CAAGTGAAGG GTGTTGCGTA TGTGCTGTTT GGTATTGAAA TTAATGGTGA AAAGAAGAGA 
CTCACATCTA TGAAGCAAAT TGATGATCTA AATGGAGGAA GTGTTAGTTT GACTATGGAG 
GAGATAAAGA GAGCTTATCC TGACATAAAC AGTCTTCTGG GGTCTACGTT GTACGTCAAA 
GCATCAGTAT TGACTAGTTC AGGTAGCGAT CTTGTGGAAG CAGAGAAATC CGGCATTAAA 
ATCGTAAAAT CACCCTACAT GCTTACCATT AAGGACACAC CAAAGTATTA TAAACCTGGT 
CTGCCGTTAG CCATGACGGT TATCGTGAGT AACCATGATG GATCTCCAAC TCCTAATATT 
CCTGTTAAAG TCACCTTTCT TGAAAGTCCA ATCAGTGTCC ACAGTGGCAC CATCAACATT 
CACATCAACA TGCCGAAAAC GCACATACTA TATGGCAATT CCCAGATATT GAAAGTGGAG 
ACAGTGGATC CGTCACTGAA ACCAGAACAG CAGGCCATTC AAGAGGTATA CGTCAAGCCA 
TATTCTACTT TTAGCGGTCA CGATAACTTC TTGCACGTCT CAGTGGGAGC AAGTCGAGTG 
GTGGTCGGTG GCAGTGTCAG TGTCCAAATG TACATCAAAT CCTCTTTACC AAACCACAGA 
GCCCTCGTGG AGCATCTCAC ATACATTGTG TTAAATAAGG GGAAAATAAT TCAGGCTGGT 
AGAGTGAATG TCAAAGGCCA GGATGTAACA AGCGTTCATC TGCTGATTAC CACAGAAATG 
CTACCTGCAT TTCGGTTTGT GGCGTACTAC GTTTTGCCAT GGCAGCATAG GGCTGAAGTG 
GTGGCAGACT CCGTGTTTGT TGATGTGGAG GACCGGTGTG TAGGTTCGCT TAATGTTGGA 
CCAGTGGATG GAGAAATAGT CAGTTCTTAC TCACCAGGCA GCAGCTTTAA ATTTCAAGTG 
AAAGGTGATG TTGGAGCAAA GGTCAGCCTG GTAGCTGTGG ATAATGCCAT CTTCTTGTTG 
AGTAAAAACC GCCTGACGCA GAAAAAGGTG TGGGAAGTGG TGGATCAGGC TGATATGGGC 
TGCACCGCAG GAGGAGGCAA GGATAATATG GGTGTTTTCA GTGATGCAGG ACTGATGTTT 
CACTCCAGCA CAGGAGGTTC AACTGACTCC AAAGAAGACT GCTCCAGTAA AACTAAACGG 
CGAAGACGTT CTGCAGCCAA ACTGCAGCTC AGAGATCAGC TCGAGAAGGC CTATTCAGAT 
GAGCTCCTGC AGAGGTGTTG TGTGGATGGC ATGAAGGAGA TTCCCATGCC GTATTCATGC 
TACCGTCGCT CTCTCTACAT CACCGAGGAC TGGAGCTGTG TGTTGGCTTT CCTGCGCTGC 
TGTGCAGAAT ACAGAGGAGA GGAGCTCGGC GTCATTACCA GACCTCCACC AACCACTGCT 
CCACCAACCA TTGCTCCGCC AACCACTTCT CCACCCACCC TCTTTCTCGT ACCACATCTT 
GAATATCATT TTCCGGAACA ACCAGTTTTT TTTGTGAGGG ATAGAGTACT ACAATCCATG 
CCAAAGCTGC ATGCCGAAGC TAATGTCGTC GGAGTTCGTC AGGACAGTTA TCGGGAAGTC 
GTTGTAGAAG AAGAAGAAGA GGCGGCTTCA GTGTTTGAGG AAGATGATTT GTCTGATCTT 
GAGGATATTT ATGTGCGCAG CAAGTTTTTT GAGTCGTGGT TGTGGACCGA TATCAGACTG 
CCTAGCACTG CCAAGTCTGA TGGGTTGGCT GTGTTTCCAG TGGACACCGT TCTTCCAGAC 
AGTATCACAC AGTGGGGGTT TCTAGCTGTC AGTGCCTCAC CTGAGAAAGG TGTCTGCGTA 
GCTGAACCGT ATAACGTCCG CGTGTGGAAG CCGTTCTTTA TCGACCTGCG TTTGCCTCAC 
TCGGTGTCCA GAAATGAACA AGTGGAAATC AAAGCTGTAA TCCACAACTA CAAGAACTCA 
ACACTGGAGG TGATGGTGAT CCTGGAGAAG ACAGAGGACA TGTGTAGTGT GGCCTATACG 
GGACAGCACA GACAGCAGGT TACAGTAGCA GCCGGTTCCT CTAAACTCAT TACATACACC 
ATCATCCCTC TGAAAACAGG GGAGCTCCCT TTACAGGTCA CTGCTGTCGC TGCCTCGTTC 
ATGGGTCAAG ATGCAGTGAA GAAAAATCTA CGCGTAGTAG TTGAAGGCAT TCAGACACTT 
AAAGTGAGGA GCTTTGTGTT AAACCCATCA GAGAAAGGAG GCAGTGCTGG GCAACAGTTG 
ATTCAAGTCA AGAAGATGCA GTTGGATGCT GTTGTGCCAA AATCTGTACC AGAAACATTT 
GTCAATGTCA GAGGCGATTT GCTGGCAGAC AGTATTGATA ACTCCATTAA GGAGGACTCG 
TTGGCAGCAT TGATCCGGAT GCCCGGAGGC TGTGTGGAGC AGAACCTGGC CAGAATCACT 
CTGCCCCTCA TCGCAGCTCA TTACCTGGAC CGCAGTGCAG ACTGGGAGAT CGTGGGAATC 
AGTCGCAGAG AAGAGGCCAT CAAATACATA CAGAGAGGTT ATGAAAATCA ACTTAACTAC 
AAAAAATCAG ACGACTCGTA CCCTCCCTAC GCCAATGAAG GCACCAGCAC ATGGATCACT 
GCGTATGTGG TGAAGGTTTT CTCCATGGCG CATTCATTCA TTAATGTCAA TGAGAAGCAT 
CTGTGTGGCC CTCTGCTCTA TTTACTCAAG AATAAACAGG GTTATGATGG AACTTTTCAA 
GAGGACAATC CCGTCTATGA CACCAGTATG ACGGGAGCTA TGCGGGGATC TGAATACAAA 
GCATCTTTGA CTGCATTTGT GCTCATTGCT TTAGCAGAGG CACAAGATAA AGTTACTTGC 
CAAGATCAAG AAGTCAACAT ACGGGATAAG TCTAGACAGG CAGCAGGGTA TCTGACAGAG 
CATTTCTCCC GGCTGACCAG GCCATATACA GCAGCTATAG CATGCTATGC GCTAGCCGTA 
TCGAACAACG CCTGCGTGAA GAGCATGTTG CTCAAATTTG CCTCACCTGA TCATTCCCAC 
TGGCCTGATT CAAACAATTA TTTTTTCACA CTGGAAGCTA CAGGCTACGC ACTGCTAGCG 
CTGATCAAAA GTGGCCACAT GGAAGAGGCA GAAGCTCCAT TTCGATGGTT GAATGAGCAC 
CGTGGCATTG GTGGAGGATA CGGCTCTACT CAGTCCACCA TGGTGGTTCT TCAGGCCCTG 
TCTGAGTATC TAGTGAAGAG GCCTCCTCCG TCAGACCTCA ATTTGTTGGT TCAGCTCAGC 
GTTCCTGGTC GGAGTGATGT TCGCTGGACC TTCAATCCCA ACTTGGCTTA TGTGGCCCGT 
TCTTCACGAG TCCCTCTTGA TCAGGAATTC TCAGTGGCGG CTTCTGGGAA CGGCAAGGGT 
GTTTTGGAGG TAGTGACGGT TTACCATCAG CTTCCTGATG TGTATGAGAA CAGCACATGC 
AACGGCTTTC AGCTGGACGT CTCTATCGCT GAGACTAATG AAAAAACCTC ATCGGATGTA 
GAAAAGTCCT TCAGACTCAA TGTCAATGTG AGGGCTCTAG AAGAACGGCA AGTGAGGATG 
GTGATTCTAG ACATCGGCCT GCCTACAGGC TTTGAACCCG AAAACTCTGA TTTAGAGTTG 
CTCACAAATT CAGTGGATCG CTACATCAAC AACTTCCAAG TGGTGGACAA CCTGAGCGAC 
AGAGGATCCC TCATAATTCA CCTGTTCAAG GTGTCCAACA AAGAGGCAGA AATGATCTCA 
TTCAGACTCA ATCAGAAATT TAAAGTGGGG CTCCTTCAGC CTTCCACAGT CAAAGTTTAC 
CAGTACTACA ACAAAGATAA AAGCTGTAGC AGGTTCTACT CTCCTCCTGA AGACAAACAG 
CAGCTGGATC AGATCTGTGA AGGCGACGTG TGCCGCTGCT CGCAGGGAGA CTGTTGTGTA 
ATGAAGGAAG ACTTTGCATT TTTTGGTAAA GAGCAGAGGA ATGCCGCTGT ATGCAACGGA 
TTACATCATG CCTACAGAGT CAAGCTGATC AGTATCAGCC AGAGTCACTA TTACCAGTAT 
GAGATGGAGA TTGTGCAGGT TATCAAAGAA GGGACGGAGG AAGGTCTGAA TGAGAAGGAG 
AGGAGGATGT TCCTCTCTCA TGCAAGCTGC AGGACCAGGC TTAATTTGAA AGAGGGTCAG 
GAGTATCTGG TCATTGGGCC GATCACTGAT GTTTGGAATG CAGGGAGCAC AGTGTCCAAC 
AAGTATGTGT ATACGCTTGG GAAAGAAACG TGGGTTGAGC GCTGGCCAGA TGAGTCTGAA 
TGTGGATCAA CACTGCAGAA GAAATGTGAT GAGCTGAAGA GCTTTGCAAA GAAACTGATT 
GAAGATGGCT GTCAGGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

C3B.1 ( XM_005174029.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_005174086.1 Danio rerio complement component c3b, tandem duplicate 1 (c3b.1), transcript variant X2, mRNA.

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CloneID	ODa27023
Clone ID Related Accession (Same CDS sequence)	XM_005174029.4 , XM_005174029.3
Accession Version	XM_005174029.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5241bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	complement component c3b, tandem duplicate 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005174029.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCTTC AGCTGCTGTT ATGGGCGACC CTGCTCTGTC AAATCTGCGA TGGAAAGGCC 
TTCCCTCAAC GACACAAGAG GACGGTTTGG AGACATATGC GACAAATTGA GCCGGTCAAA 
GACCCTTCTC CGTCGTTTTC TCTTCTAGCT CCAGCAAAAA TAAGGCCTGG AACAAAACAG 
AACATCCTGG TGGAGGGACA CAACCTGGCA CAGGTGGTAG AAGTCACTGT TGTGGTTTAC 
GACTATCCAA ACTCCCAAAC TGCCTTGTCA CGGGGCTCAG TTTTCCTCAA TTCAGACAAC 
AACTACAGCG TCCTGCAAAC CCTAGAGATC AACCCAAATT TGCTACTTCC AGATGGAAAA 
AAGAAGTATG TGAAGCTCGT TGCTCAGTTC AGCAACATCT ATCGTGCAGA AAAAGTAATG 
GCGGTGTCGT TCCGCTCCGG ACACATCTTC ATTCAGACCG ACAAACCTAT TTACAACCCT 
GGAGACAAAG TACGTTTCAG GGCTTTTGTG TCTGATTTGG AATTTCGTTC TTCTGAGAGA 
ACCATATCTC TGGAGATTCA GAATCCAGAT GGAATTGCGG TGCATGGTAT TGGGAGAGTT 
AAAGCAATCG ATGGGGTCTT TTCTGACATT TTCTCACTGT CTACTGTAGT CAAAGAGGGC 
AGATGGAAAG TTGTAGCAAA GTTTGATCAG GGAAAAGAGA ATGTTTTCAG CAGAGAGTTT 
GAAGTGAAGA AATATGTGCT TCCAGCATTC AACGTCACAC TTACTCCTAA AACATCCCAC 
CTTAGTCTGG ATGCAGAGAA GTTGGAAGTT GAAATAACAG CTAGGTATCT TTATGGCGCG 
CAAGTGAAGG GTGTTGCGTA TGTGCTGTTT GGTATTGAAA TTAATGGTGA AAAGAAGAGA 
CTCACATCTA TGAAGCAAAT TGATGATCTA AATGGAGGAA GTGTTAGTTT GACTATGGAG 
GAGATAAAGA GAGCTTATCC TGACATAAAC AGTCTTCTGG GGTCTACGTT GTACGTCAAA 
GCATCAGTAT TGACTAGTTC AGGTAGCGAT CTTGTGGAAG CAGAGAAATC CGGCATTAAA 
ATCGTAAAAT CACCCTACAT GCTTACCATT AAGGACACAC CAAAGTATTA TAAACCTGGT 
CTGCCGTTAG CCATGACGGT TATCGTGAGT AACCATGATG GATCTCCAAC TCCTAATATT 
CCTGTTAAAG TCACCTTTCT TGAAAGTCCA ATCAGTGTCC ACAGTGGCAC CATCAACATT 
CACATCAACA TGCCGAAAAC GCACATACTA TATGGCAATT CCCAGATATT GAAAGTGGAG 
ACAGTGGATC CGTCACTGAA ACCAGAACAG CAGGCCATTC AAGAGGTATA CGTCAAGCCA 
TATTCTACTT TTAGCGGTCA CGATAACTTC TTGCACGTCT CAGTGGGAGC AAGTCGAGTG 
GTGGTCGGTG GCAGTGTCAG TGTCCAAATG TACATCAAAT CCTCTTTACC AAACCACAGA 
GCCCTCGTGG AGCATCTCAC ATACATTGTG TTAAATAAGG GGAAAATAAT TCAGGCTGGT 
AGAGTGAATG TCAAAGGCCA GGATGTAACA AGCGTTCATC TGCTGATTAC CACAGAAATG 
CTACCTGCAT TTCGGTTTGT GGCGTACTAC GTTTTGCCAT GGCAGCATAG GGCTGAAGTG 
GTGGCAGACT CCGTGTTTGT TGATGTGGAG GACCGGTGTG TAGGTTCGCT TAATGTTGGA 
CCAGTGGATG GAGAAATAGT CAGTTCTTAC TCACCAGGCA GCAGCTTTAA ATTTCAAGTG 
AAAGGTGATG TTGGAGCAAA GGTCAGCCTG GTAGCTGTGG ATAATGCCAT CTTCTTGTTG 
AGTAAAAACC GCCTGACGCA GAAAAAGGTG TGGGAAGTGG TGGATCAGGC TGATATGGGC 
TGCACCGCAG GAGGAGGCAA GGATAATATG GGTGTTTTCA GTGATGCAGG ACTGATGTTT 
CACTCCAGCA CAGGAGGTTC AACTGACTCC AAAGAAGACT GCTCCAGTAA AACTAAACGG 
CGAAGACGTT CTGCAGCCAA ACTGCAGCTC AGAGATCAGC TCGAGAAGGC CTATTCAGAT 
GAGCTCCTGC AGAGGTGTTG TGTGGATGGC ATGAAGGAGA TTCCCATGCC GTATTCATGC 
TACCGTCGCT CTCTCTACAT CACCGAGGAC TGGAGCTGTG TGTTGGCTTT CCTGCGCTGC 
TGTGCAGAAT ACAGAGGAGA GGAGCTCGGC GTCATTACCA GACCTCCACC AACCACTGCT 
CCACCAACCA TTGCTCCGCC AACCACTTCT CCACCCACCC TCTTTCTCGT ACCACATCTT 
GAATATCATT TTCCGGAACA ACCAGTTTTT TTTGTGAGGG ATAGAGTACT ACAATCCATG 
CCAAAGCTGC ATGCCGAAGC TAATGTCGTC GGAGTTCGTC AGGACAGTTA TCGGGAAGTC 
GTTGTAGAAG AAGAAGAAGA GGCGGCTTCA GTGTTTGAGG AAGATGATTT GTCTGATCTT 
GAGGATATTT ATGTGCGCAG CAAGTTTTTT GAGTCGTGGT TGTGGACCGA TATCAGACTG 
CCTAGCACTG CCAAGTCTGA TGGGTTGGCT GTGTTTCCAG TGGACACCGT TCTTCCAGAC 
AGTATCACAC AGTGGGGGTT TCTAGCTGTC AGTGCCTCAC CTGAGAAAGG TGTCTGCGTA 
GCTGAACCGT ATAACGTCCG CGTGTGGAAG CCGTTCTTTA TCGACCTGCG TTTGCCTCAC 
TCGGTGTCCA GAAATGAACA AGTGGAAATC AAAGCTGTAA TCCACAACTA CAAGAACTCA 
ACACTGGAGG TGATGGTGAT CCTGGAGAAG ACAGAGGACA TGTGTAGTGT GGCCTATACG 
GGACAGCACA GACAGCAGGT TACAGTAGCA GCCGGTTCCT CTAAACTCAT TACATACACC 
ATCATCCCTC TGAAAACAGG GGAGCTCCCT TTACAGGTCA CTGCTGTCGC TGCCTCGTTC 
ATGGGTCAAG ATGCAGTGAA GAAAAATCTA CGCGTAGTAG TTGAAGGCAT TCAGACACTT 
AAAGTGAGGA GCTTTGTGTT AAACCCATCA GAGAAAGGAG GCAGTGCTGG GCAACAGTTG 
ATTCAAGTCA AGAAGATGCA GTTGGATGCT GTTGTGCCAA AATCTGTACC AGAAACATTT 
GTCAATGTCA GAGGCGATTT GCTGGCAGAC AGTATTGATA ACTCCATTAA GGAGGACTCG 
TTGGCAGCAT TGATCCGGAT GCCCGGAGGC TGTGTGGAGC AGAACCTGGC CAGAATCACT 
CTGCCCCTCA TCGCAGCTCA TTACCTGGAC CGCAGTGCAG ACTGGGAGAT CGTGGGAATC 
AGTCGCAGAG AAGAGGCCAT CAAATACATA CAGAGAGGTT ATGAAAATCA ACTTAACTAC 
AAAAAATCAG ACGACTCGTA CCCTCCCTAC GCCAATGAAG GCACCAGCAC ATGGATCACT 
GCGTATGTGG TGAAGGTTTT CTCCATGGCG CATTCATTCA TTAATGTCAA TGAGAAGCAT 
CTGTGTGGCC CTCTGCTCTA TTTACTCAAG AATAAACAGG GTTATGATGG AACTTTTCAA 
GAGGACAATC CCGTCTATGA CACCAGTATG ACGGGAGCTA TGCGGGGATC TGAATACAAA 
GCATCTTTGA CTGCATTTGT GCTCATTGCT TTAGCAGAGG CACAAGATAA AGTTACTTGC 
CAAGATCAAG AAGTCAACAT ACGGGATAAG TCTAGACAGG CAGCAGGGTA TCTGACAGAG 
CATTTCTCCC GGCTGACCAG GCCATATACA GCAGCTATAG CATGCTATGC GCTAGCCGTA 
TCGAACAACG CCTGCGTGAA GAGCATGTTG CTCAAATTTG CCTCACCTGA TCATTCCCAC 
TGGCCTGATT CAAACAATTA TTTTTTCACA CTGGAAGCTA CAGGCTACGC ACTGCTAGCG 
CTGATCAAAA GTGGCCACAT GGAAGAGGCA GAAGCTCCAT TTCGATGGTT GAATGAGCAC 
CGTGGCATTG GTGGAGGATA CGGCTCTACT CAGTCCACCA TGGTGGTTCT TCAGGCCCTG 
TCTGAGTATC TAGTGAAGAG GCCTCCTCCG TCAGACCTCA ATTTGTTGGT TCAGCTCAGC 
GTTCCTGGTC GGAGTGATGT TCGCTGGACC TTCAATCCCA ACTTGGCTTA TGTGGCCCGT 
TCTTCACGAG TCCCTCTTGA TCAGGAATTC TCAGTGGCGG CTTCTGGGAA CGGCAAGGGT 
GTTTTGGAGG TAGTGACGGT TTACCATCAG CTTCCTGATG TGTATGAGAA CAGCACATGC 
AACGGCTTTC AGCTGGACGT CTCTATCGCT GAGACTAATG AAAAAACCTC ATCGGATGTA 
GAAAAGTCCT TCAGACTCAA TGTCAATGTG AGGGCTCTAG AAGAACGGCA AGTGAGGATG 
GTGATTCTAG ACATCGGCCT GCCTACAGGC TTTGAACCCG AAAACTCTGA TTTAGAGTTG 
CTCACAAATT CAGTGGATCG CTACATCAAC AACTTCCAAG TGGTGGACAA CCTGAGCGAC 
AGAGGATCCC TCATAATTCA CCTGTTCAAG GTGTCCAACA AAGAGGCAGA AATGATCTCA 
TTCAGACTCA ATCAGAAATT TAAAGTGGGG CTCCTTCAGC CTTCCACAGT CAAAGTTTAC 
CAGTACTACA ACAAAGATAA AAGCTGTAGC AGGTTCTACT CTCCTCCTGA AGACAAACAG 
CAGCTGGATC AGATCTGTGA AGGCGACGTG TGCCGCTGCT CGCAGGGAGA CTGTTGTGTA 
ATGAAGGAAG ACTTTGCATT TTTTGGTAAA GAGCAGAGGA ATGCCGCTGT ATGCAACGGA 
TTACATCATG CCTACAGAGT CAAGCTGATC AGTATCAGCC AGAGTCACTA TTACCAGTAT 
GAGATGGAGA TTGTGCAGGT TATCAAAGAA GGGACGGAGG AAGGTCTGAA TGAGAAGGAG 
AGGAGGATGT TCCTCTCTCA TGCAAGCTGC AGGACCAGGC TTAATTTGAA AGAGGGTCAG 
GAGTATCTGG TCATTGGGCC GATCACTGAT GTTTGGAATG CAGGGAGCAC AGTGTCCAAC 
AAGTATGTGT ATACGCTTGG GAAAGAAACG TGGGTTGAGC GCTGGCCAGA TGAGTCTGAA 
TGTGGATCAA CACTGCAGAA GAAATGTGAT GAGCTGAAGA GCTTTGCAAA GAAACTGATT 
GAAGATGGCT GTCAGGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005174086.1
CDS	124..5364
Translation	MLLQLLLWATLLCQICDGKAFPQRHKRTVWRHMRQIEPVKDPSPSFSLLAPAKIRPGTKQNILVEGHNLAQVVEVTVVVYDYPNSQTALSRGSVFLNSDNNYSVLQTLEINPNLLLPDGKKKYVKLVAQFSNIYRAEKVMAVSFRSGHIFIQTDKPIYNPGDKVRFRAFVSDLEFRSSERTISLEIQNPDGIAVHGIGRVKAIDGVFSDIFSLSTVVKEGRWKVVAKFDQGKENVFSREFEVKKYVLPAFNVTLTPKTSHLSLDAEKLEVEITARYLYGAQVKGVAYVLFGIEINGEKKRLTSMKQIDDLNGGSVSLTMEEIKRAYPDINSLLGSTLYVKASVLTSSGSDLVEAEKSGIKIVKSPYMLTIKDTPKYYKPGLPLAMTVIVSNHDGSPTPNIPVKVTFLESPISVHSGTINIHINMPKTHILYGNSQILKVETVDPSLKPEQQAIQEVYVKPYSTFSGHDNFLHVSVGASRVVVGGSVSVQMYIKSSLPNHRALVEHLTYIVLNKGKIIQAGRVNVKGQDVTSVHLLITTEMLPAFRFVAYYVLPWQHRAEVVADSVFVDVEDRCVGSLNVGPVDGEIVSSYSPGSSFKFQVKGDVGAKVSLVAVDNAIFLLSKNRLTQKKVWEVVDQADMGCTAGGGKDNMGVFSDAGLMFHSSTGGSTDSKEDCSSKTKRRRRSAAKLQLRDQLEKAYSDELLQRCCVDGMKEIPMPYSCYRRSLYITEDWSCVLAFLRCCAEYRGEELGVITRPPPTTAPPTIAPPTTSPPTLFLVPHLEYHFPEQPVFFVRDRVLQSMPKLHAEANVVGVRQDSYREVVVEEEEEAASVFEEDDLSDLEDIYVRSKFFESWLWTDIRLPSTAKSDGLAVFPVDTVLPDSITQWGFLAVSASPEKGVCVAEPYNVRVWKPFFIDLRLPHSVSRNEQVEIKAVIHNYKNSTLEVMVILEKTEDMCSVAYTGQHRQQVTVAAGSSKLITYTIIPLKTGELPLQVTAVAASFMGQDAVKKNLRVVVEGIQTLKVRSFVLNPSEKGGSAGQQLIQVKKMQLDAVVPKSVPETFVNVRGDLLADSIDNSIKEDSLAALIRMPGGCVEQNLARITLPLIAAHYLDRSADWEIVGISRREEAIKYIQRGYENQLNYKKSDDSYPPYANEGTSTWITAYVVKVFSMAHSFINVNEKHLCGPLLYLLKNKQGYDGTFQEDNPVYDTSMTGAMRGSEYKASLTAFVLIALAEAQDKVTCQDQEVNIRDKSRQAAGYLTEHFSRLTRPYTAAIACYALAVSNNACVKSMLLKFASPDHSHWPDSNNYFFTLEATGYALLALIKSGHMEEAEAPFRWLNEHRGIGGGYGSTQSTMVVLQALSEYLVKRPPPSDLNLLVQLSVPGRSDVRWTFNPNLAYVARSSRVPLDQEFSVAASGNGKGVLEVVTVYHQLPDVYENSTCNGFQLDVSIAETNEKTSSDVEKSFRLNVNVRALEERQVRMVILDIGLPTGFEPENSDLELLTNSVDRYINNFQVVDNLSDRGSLIIHLFKVSNKEAEMISFRLNQKFKVGLLQPSTVKVYQYYNKDKSCSRFYSPPEDKQQLDQICEGDVCRCSQGDCCVMKEDFAFFGKEQRNAAVCNGLHHAYRVKLISISQSHYYQYEMEIVQVIKEGTEEGLNEKERRMFLSHASCRTRLNLKEGQEYLVIGPITDVWNAGSTVSNKYVYTLGKETWVERWPDESECGSTLQKKCDELKSFAKKLIEDGCQA

Target ORF information:

RefSeq Version	XM_005174029.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio complement component c3b, tandem duplicate 1 (c3b.1), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005174029.3

ORF Insert Sequence:

ATGCTTCTTC AGCTGCTGTT ATGGGCGACC CTGCTCTGTC AAATCTGCGA TGGAAAGGCC 
TTCCCTCAAC GACACAAGAG GACGGTTTGG AGACATATGC GACAAATTGA GCCGGTCAAA 
GACCCTTCTC CGTCGTTTTC TCTTCTAGCT CCAGCAAAAA TAAGGCCTGG AACAAAACAG 
AACATCCTGG TGGAGGGACA CAACCTGGCA CAGGTGGTAG AAGTCACTGT TGTGGTTTAC 
GACTATCCAA ACTCCCAAAC TGCCTTGTCA CGGGGCTCAG TTTTCCTCAA TTCAGACAAC 
AACTACAGCG TCCTGCAAAC CCTAGAGATC AACCCAAATT TGCTACTTCC AGATGGAAAA 
AAGAAGTATG TGAAGCTCGT TGCTCAGTTC AGCAACATCT ATCGTGCAGA AAAAGTAATG 
GCGGTGTCGT TCCGCTCCGG ACACATCTTC ATTCAGACCG ACAAACCTAT TTACAACCCT 
GGAGACAAAG TACGTTTCAG GGCTTTTGTG TCTGATTTGG AATTTCGTTC TTCTGAGAGA 
ACCATATCTC TGGAGATTCA GAATCCAGAT GGAATTGCGG TGCATGGTAT TGGGAGAGTT 
AAAGCAATCG ATGGGGTCTT TTCTGACATT TTCTCACTGT CTACTGTAGT CAAAGAGGGC 
AGATGGAAAG TTGTAGCAAA GTTTGATCAG GGAAAAGAGA ATGTTTTCAG CAGAGAGTTT 
GAAGTGAAGA AATATGTGCT TCCAGCATTC AACGTCACAC TTACTCCTAA AACATCCCAC 
CTTAGTCTGG ATGCAGAGAA GTTGGAAGTT GAAATAACAG CTAGGTATCT TTATGGCGCG 
CAAGTGAAGG GTGTTGCGTA TGTGCTGTTT GGTATTGAAA TTAATGGTGA AAAGAAGAGA 
CTCACATCTA TGAAGCAAAT TGATGATCTA AATGGAGGAA GTGTTAGTTT GACTATGGAG 
GAGATAAAGA GAGCTTATCC TGACATAAAC AGTCTTCTGG GGTCTACGTT GTACGTCAAA 
GCATCAGTAT TGACTAGTTC AGGTAGCGAT CTTGTGGAAG CAGAGAAATC CGGCATTAAA 
ATCGTAAAAT CACCCTACAT GCTTACCATT AAGGACACAC CAAAGTATTA TAAACCTGGT 
CTGCCGTTAG CCATGACGGT TATCGTGAGT AACCATGATG GATCTCCAAC TCCTAATATT 
CCTGTTAAAG TCACCTTTCT TGAAAGTCCA ATCAGTGTCC ACAGTGGCAC CATCAACATT 
CACATCAACA TGCCGAAAAC GCACATACTA TATGGCAATT CCCAGATATT GAAAGTGGAG 
ACAGTGGATC CGTCACTGAA ACCAGAACAG CAGGCCATTC AAGAGGTATA CGTCAAGCCA 
TATTCTACTT TTAGCGGTCA CGATAACTTC TTGCACGTCT CAGTGGGAGC AAGTCGAGTG 
GTGGTCGGTG GCAGTGTCAG TGTCCAAATG TACATCAAAT CCTCTTTACC AAACCACAGA 
GCCCTCGTGG AGCATCTCAC ATACATTGTG TTAAATAAGG GGAAAATAAT TCAGGCTGGT 
AGAGTGAATG TCAAAGGCCA GGATGTAACA AGCGTTCATC TGCTGATTAC CACAGAAATG 
CTACCTGCAT TTCGGTTTGT GGCGTACTAC GTTTTGCCAT GGCAGCATAG GGCTGAAGTG 
GTGGCAGACT CCGTGTTTGT TGATGTGGAG GACCGGTGTG TAGGTTCGCT TAATGTTGGA 
CCAGTGGATG GAGAAATAGT CAGTTCTTAC TCACCAGGCA GCAGCTTTAA ATTTCAAGTG 
AAAGGTGATG TTGGAGCAAA GGTCAGCCTG GTAGCTGTGG ATAATGCCAT CTTCTTGTTG 
AGTAAAAACC GCCTGACGCA GAAAAAGGTG TGGGAAGTGG TGGATCAGGC TGATATGGGC 
TGCACCGCAG GAGGAGGCAA GGATAATATG GGTGTTTTCA GTGATGCAGG ACTGATGTTT 
CACTCCAGCA CAGGAGGTTC AACTGACTCC AAAGAAGACT GCTCCAGTAA AACTAAACGG 
CGAAGACGTT CTGCAGCCAA ACTGCAGCTC AGAGATCAGC TCGAGAAGGC CTATTCAGAT 
GAGCTCCTGC AGAGGTGTTG TGTGGATGGC ATGAAGGAGA TTCCCATGCC GTATTCATGC 
TACCGTCGCT CTCTCTACAT CACCGAGGAC TGGAGCTGTG TGTTGGCTTT CCTGCGCTGC 
TGTGCAGAAT ACAGAGGAGA GGAGCTCGGC GTCATTACCA GACCTCCACC AACCACTGCT 
CCACCAACCA TTGCTCCGCC AACCACTTCT CCACCCACCC TCTTTCTCGT ACCACATCTT 
GAATATCATT TTCCGGAACA ACCAGTTTTT TTTGTGAGGG ATAGAGTACT ACAATCCATG 
CCAAAGCTGC ATGCCGAAGC TAATGTCGTC GGAGTTCGTC AGGACAGTTA TCGGGAAGTC 
GTTGTAGAAG AAGAAGAAGA GGCGGCTTCA GTGTTTGAGG AAGATGATTT GTCTGATCTT 
GAGGATATTT ATGTGCGCAG CAAGTTTTTT GAGTCGTGGT TGTGGACCGA TATCAGACTG 
CCTAGCACTG CCAAGTCTGA TGGGTTGGCT GTGTTTCCAG TGGACACCGT TCTTCCAGAC 
AGTATCACAC AGTGGGGGTT TCTAGCTGTC AGTGCCTCAC CTGAGAAAGG TGTCTGCGTA 
GCTGAACCGT ATAACGTCCG CGTGTGGAAG CCGTTCTTTA TCGACCTGCG TTTGCCTCAC 
TCGGTGTCCA GAAATGAACA AGTGGAAATC AAAGCTGTAA TCCACAACTA CAAGAACTCA 
ACACTGGAGG TGATGGTGAT CCTGGAGAAG ACAGAGGACA TGTGTAGTGT GGCCTATACG 
GGACAGCACA GACAGCAGGT TACAGTAGCA GCCGGTTCCT CTAAACTCAT TACATACACC 
ATCATCCCTC TGAAAACAGG GGAGCTCCCT TTACAGGTCA CTGCTGTCGC TGCCTCGTTC 
ATGGGTCAAG ATGCAGTGAA GAAAAATCTA CGCGTAGTAG TTGAAGGCAT TCAGACACTT 
AAAGTGAGGA GCTTTGTGTT AAACCCATCA GAGAAAGGAG GCAGTGCTGG GCAACAGTTG 
ATTCAAGTCA AGAAGATGCA GTTGGATGCT GTTGTGCCAA AATCTGTACC AGAAACATTT 
GTCAATGTCA GAGGCGATTT GCTGGCAGAC AGTATTGATA ACTCCATTAA GGAGGACTCG 
TTGGCAGCAT TGATCCGGAT GCCCGGAGGC TGTGTGGAGC AGAACCTGGC CAGAATCACT 
CTGCCCCTCA TCGCAGCTCA TTACCTGGAC CGCAGTGCAG ACTGGGAGAT CGTGGGAATC 
AGTCGCAGAG AAGAGGCCAT CAAATACATA CAGAGAGGTT ATGAAAATCA ACTTAACTAC 
AAAAAATCAG ACGACTCGTA CCCTCCCTAC GCCAATGAAG GCACCAGCAC ATGGATCACT 
GCGTATGTGG TGAAGGTTTT CTCCATGGCG CATTCATTCA TTAATGTCAA TGAGAAGCAT 
CTGTGTGGCC CTCTGCTCTA TTTACTCAAG AATAAACAGG GTTATGATGG AACTTTTCAA 
GAGGACAATC CCGTCTATGA CACCAGTATG ACGGGAGCTA TGCGGGGATC TGAATACAAA 
GCATCTTTGA CTGCATTTGT GCTCATTGCT TTAGCAGAGG CACAAGATAA AGTTACTTGC 
CAAGATCAAG AAGTCAACAT ACGGGATAAG TCTAGACAGG CAGCAGGGTA TCTGACAGAG 
CATTTCTCCC GGCTGACCAG GCCATATACA GCAGCTATAG CATGCTATGC GCTAGCCGTA 
TCGAACAACG CCTGCGTGAA GAGCATGTTG CTCAAATTTG CCTCACCTGA TCATTCCCAC 
TGGCCTGATT CAAACAATTA TTTTTTCACA CTGGAAGCTA CAGGCTACGC ACTGCTAGCG 
CTGATCAAAA GTGGCCACAT GGAAGAGGCA GAAGCTCCAT TTCGATGGTT GAATGAGCAC 
CGTGGCATTG GTGGAGGATA CGGCTCTACT CAGTCCACCA TGGTGGTTCT TCAGGCCCTG 
TCTGAGTATC TAGTGAAGAG GCCTCCTCCG TCAGACCTCA ATTTGTTGGT TCAGCTCAGC 
GTTCCTGGTC GGAGTGATGT TCGCTGGACC TTCAATCCCA ACTTGGCTTA TGTGGCCCGT 
TCTTCACGAG TCCCTCTTGA TCAGGAATTC TCAGTGGCGG CTTCTGGGAA CGGCAAGGGT 
GTTTTGGAGG TAGTGACGGT TTACCATCAG CTTCCTGATG TGTATGAGAA CAGCACATGC 
AACGGCTTTC AGCTGGACGT CTCTATCGCT GAGACTAATG AAAAAACCTC ATCGGATGTA 
GAAAAGTCCT TCAGACTCAA TGTCAATGTG AGGGCTCTAG AAGAACGGCA AGTGAGGATG 
GTGATTCTAG ACATCGGCCT GCCTACAGGC TTTGAACCCG AAAACTCTGA TTTAGAGTTG 
CTCACAAATT CAGTGGATCG CTACATCAAC AACTTCCAAG TGGTGGACAA CCTGAGCGAC 
AGAGGATCCC TCATAATTCA CCTGTTCAAG GTGTCCAACA AAGAGGCAGA AATGATCTCA 
TTCAGACTCA ATCAGAAATT TAAAGTGGGG CTCCTTCAGC CTTCCACAGT CAAAGTTTAC 
CAGTACTACA ACAAAGATAA AAGCTGTAGC AGGTTCTACT CTCCTCCTGA AGACAAACAG 
CAGCTGGATC AGATCTGTGA AGGCGACGTG TGCCGCTGCT CGCAGGGAGA CTGTTGTGTA 
ATGAAGGAAG ACTTTGCATT TTTTGGTAAA GAGCAGAGGA ATGCCGCTGT ATGCAACGGA 
TTACATCATG CCTACAGAGT CAAGCTGATC AGTATCAGCC AGAGTCACTA TTACCAGTAT 
GAGATGGAGA TTGTGCAGGT TATCAAAGAA GGGACGGAGG AAGGTCTGAA TGAGAAGGAG 
AGGAGGATGT TCCTCTCTCA TGCAAGCTGC AGGACCAGGC TTAATTTGAA AGAGGGTCAG 
GAGTATCTGG TCATTGGGCC GATCACTGAT GTTTGGAATG CAGGGAGCAC AGTGTCCAAC 
AAGTATGTGT ATACGCTTGG GAAAGAAACG TGGGTTGAGC GCTGGCCAGA TGAGTCTGAA 
TGTGGATCAA CACTGCAGAA GAAATGTGAT GAGCTGAAGA GCTTTGCAAA GAAACTGATT 
GAAGATGGCT GTCAGGCTTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

C3B.1 ( XM_009296389.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009294664.1 Danio rerio complement component c3b, tandem duplicate 1 (c3b.1), transcript variant X1, mRNA.

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CloneID	ODa44129
Clone ID Related Accession (Same CDS sequence)	XM_009296389.3 , XM_009296389.2
Accession Version	XM_009296389.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	complement component c3b, tandem duplicate 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296389.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCTTC AGCTGCTGTT ATGGGCGACC CTGCTCTGTC AAATCTGCGA TGGAAAGGCC 
TTCCCTCAAC GACACAAGAG GACGGTTTGG AGACATATGC GACAAATTGA GCCGGTCAAA 
GACCCTTCTC CGTCGTTTTC TCTTCTAGCT CCAGCAAAAA TAAGGCCTGG AACAAAACAG 
AACATCCTGG TGGAGGGACA CAACCTGGCA CAGGTGGTAG AAGTCACTGT TGTGGTTTAC 
GACTATCCAA ACTCCCAAAC TGCCTTGTCA CGGGGCTCAG TTTTCCTCAA TTCAGACAAC 
AACTACAGCG TCCTGCAAAC CCTAGAGATC AACCCAAATT TGCTACTTCC AGATGGAAAA 
AAGAAGTATG TGAAGCTCGT TGCTCAGTTC AGCAACATCT ATCGTGCAGA AAAAGTAATG 
GCGGTGTCGT TCCGCTCCGG ACACATCTTC ATTCAGACCG ACAAACCTAT TTACAACCCT 
GGAGACAAAG TACGTTTCAG GGCTTTTGTG TCTGATTTGG AATTTCGTTC TTCTGAGAGA 
ACCATATCTC TGGAGATTCA GAATCCAGAT GGAATTGCGG TGCATGGTAT TGGGAGAGTT 
AAAGCAATCG ATGGGGTCTT TTCTGACATT TTCTCACTGT CTACTGTAGT CAAAGAGGGC 
AGATGGAAAG TTGTAGCAAA GTTTGATCAG GGAAAAGAGA ATGTTTTCAG CAGAGAGTTT 
GAAGTGAAGA AATATGTGCT TCCAGCATTC AACGTCACAC TTACTCCTAA AACATCCCAC 
CTTAGTCTGG ATGCAGAGAA GTTGGAAGTT GAAATAACAG CTAGGTATCT TTATGGCGCG 
CAAGTGAAGG GTGTTGCGTA TGTGCTGTTT GGTATTGAAA TTAATGGTGA AAAGAAGAGA 
CTCACATCTA TGAAGCAAAT TGATGATCTA AATGGAGGAA GTGTTAGTTT GACTATGGAG 
GAGATAAAGA GAGCTTATCC TGACATAAAC AGTCTTCTGG GGTCTACGTT GTACGTCAAA 
GCATCAGTAT TGACTAGTTC AGGTAGCGAT CTTGTGGAAG CAGAGAAATC CGGCATTAAA 
ATCGTAAAAT CACCCTACAT GCTTACCATT AAGGACACAC CAAAGTATTA TAAACCTGGT 
CTGCCGTTAG CCATGACGGT TATCGTGAGT AACCATGATG GATCTCCAAC TCCTAATATT 
CCTGTTAAAG TCACCTTTCT TGAAAGTCCA ATCAGTGTCC ACAGTGGCAC CATCAACATT 
CACATCAACA TGCCGAAAAC GCACATACTA TATGGCAATT CCCAGATATT GAAAGTGGAG 
ACAGTGGATC CGTCACTGAA ACCAGAACAG CAGGCCATTC AAGAGGTATA CGTCAAGCCA 
TATTCTACTT TTAGCGGTCA CGATAACTTC TTGCACGTCT CAGTGGGAGC AAGTCGAGTG 
GTGGTCGGTG GCAGTGTCAG TGTCCAAATG TACATCAAAT CCTCTTTACC AAACCACAGA 
GCCCTCGTGG AGCATCTCAC ATACATTGTG TTAAATAAGG GGAAAATAAT TCAGGCTGGT 
AGAGTGAATG TCAAAGGCCA GGATGTAACA AGCGTTCATC TGCTGATTAC CACAGAAATG 
CTACCTGCAT TTCGGTTTGT GGCGTACTAC GTTTTGCCAT GGCAGCATAG GGCTGAAGTG 
GTGGCAGACT CCGTGTTTGT TGATGTGGAG GACCGGTGTG TAGGTTCGCT TAATGTTGGA 
CCAGTGGATG GAGAAATAGT CAGTTCTTAC TCACCAGGCA GCAGCTTTAA ATTTCAAGTG 
AAAGGTGATG TTGGAGCAAA GGTCAGCCTG GTAGCTGTGG ATAATGCCAT CTTCTTGTTG 
AGTAAAAACC GCCTGACGCA GAAAAAGGTG TGGGAAGTGG TGGATCAGGC TGATATGGGC 
TGCACCGCAG GAGGAGGCAA GGATAATATG GGTGTTTTCA GTGATGCAGG ACTGATGTTT 
CACTCCAGCA CAGGAGGTTC AACTGACTCC AAAGAAGACT GCTCCAGTAA AACTAAACGG 
CGAAGACGTT CTGCAGCCAA ACTGCAGCTC AGAGATCAGC TCGAGAAGGC CTATTCAGAT 
GAGCTCCTGC AGAGGTGTTG TGTGGATGGC ATGAAGGAGA TTCCCATGCC GTATTCATGC 
TACCGTCGCT CTCTCTACAT CACCGAGGAC TGGAGCTGTG TGTTGGCTTT CCTGCGCTGC 
TGTGCAGAAT ACAGAGGAGA GGAGCTCGGC GTCATTACCA GACCTCCACC AACCACTGCT 
CCACCAACCA TTGCTCCGCC AACCACTTCT CCACCCACCC TCTTTCTCGT ACCACATCTT 
GAATATCATT TTCCGGAACA ACCAGTTTTT TTTGTGAGGG ATAGAGTACT ACAATCCATG 
CCAAAGCTGC ATGCCGAAGC TAATGTCGTC GGAGTTCGTC AGGACAGTTA TCGGGAAGTC 
GTTGTAGAAG AAGAAGAAGA GGCGGCTTCA GTGTTTGAGG AAGATGATTT GTCTGATCTT 
GAGGATATTT ATGTGCGCAG CAAGTTTTTT GAGTCGTGGT TGTGGACCGA TATCAGACTG 
CCTAGCACTG CCAAGTCTGA TGGGTTGGCT GTGTTTCCAG TGGACACCGT TCTTCCAGAC 
AGTATCACAC AGTGGGGGTT TCTAGCTGTC AGTGCCTCAC CTGAGAAAGG TGTCTGCGTA 
GCTGAACCGT ATAACGTCCG CGTGTGGAAG CCGTTCTTTA TCGACCTGCG TTTGCCTCAC 
TCGGTGTCCA GAAATGAACA AGTGGAAATC AAAGCTGTAA TCCACAACTA CAAGAACTCA 
ACACTGGAGG TGATGGTGAT CCTGGAGAAG ACAGAGGACA TGTGTAGTGT GGCCTATACG 
GGACAGCACA GACAGCAGGT TACAGTAGCA GCCGGTTCCT CTAAACTCAT TACATACACC 
ATCATCCCTC TGAAAACAGG GGAGCTCCCT TTACAGGTCA CTGCTGTCGC TGCCTCGTTC 
ATGGGTCAAG ATGCAGTGAA GAAAAATCTA CGCGTAGTAG TTGAAGGCAT TCAGACACTT 
AAAGTGAGGA GCTTTGTGTT AAACCCATCA GAGAAAGGAG GCAGTGCTGG GCAACAGTTG 
ATTCAAGTCA AGAAGATGCA GTTGGATGCT GTTGTGCCAA AATCTGTACC AGAAACATTT 
GTCAATGTCA GAGGCGATTT GCTGGCAGAC AGTATTGATA ACTCCATTAA GGAGGACTCG 
TTGGCAGCAT TGATCCGGAT GCCCGGAGGC TGTGTGGAGC AGAACCTGGC CAGAATCACT 
CTGCCCCTCA TCGCAGCTCA TTACCTGGAC CGCAGTGCAG ACTGGGAGAT CGTGGGAATC 
AGTCGCAGAG AAGAGGCCAT CAAATACATA CAGAGAGGTT ATGAAAATCA ACTTAACTAC 
AAAAAATCAG ACGACTCGTA CCCTCCCTAC GCCAATGAAG GCACCAGCAC ATGGATCACT 
GCGTATGTGG TGAAGGTTTT CTCCATGGCG CATTCATTCA TTAATGTCAA TGAGAAGCAT 
CTGTGTGGCC CTCTGCTCTA TTTACTCAAG AATAAACAGG GTTATGATGG AACTTTTCAA 
GAGGACAATC CCGTCTATGA CACCAGTATG ACGGGAGCTA TGCGGGGATC TGAATACAAA 
GCATCTTTGA CTGCATTTGT GCTCATTGCT TTAGCAGAGG CACAAGATAA AGTTACTTGC 
CAAGATCAAG AAGTCAACAT ACGGGATAAG TCTAGACAGG CAGCAGGGTA TCTGACAGAG 
CATTTCTCCC GGCTGACCAG GCCATATACA GCAGCTATAG CATGCTATGC GCTAGCCGTA 
TCGAACAACG CCTGCGTGAA GAGCATGTTG CTCAAATTTG CCTCACCTGA TCATTCCCAC 
TGGCCTGATT CAAACAATTA TTTTTTCACA CTGGAAGCTA CAGGCTACGC ACTGCTAGCG 
CTGATCAAAA GTGGCCACAT GGAAGAGGCA GAAGCTCCAT TTCGATGGTT GAATGAGCAC 
CGTGGCATTG GTGGAGGATA CGGCTCTACT CAGTCCACCA TGGTGGTTCT TCAGGCCCTG 
TCTGAGTATC TAGTGAAGAG GCCTCCTCCG TCAGACCTCA ATTTGTTGGT TCAGCTCAGC 
GTTCCTGGTC GGAGTGATGT TCGCTGGACC TTCAATCCCA ACTTGGCTTA TGTGGCCCGT 
TCTTCACGAG TCCCTCTTGA TCAGGAATTC TCAGTGGCGG CTTCTGGGAA CGGCAAGGGT 
GTTTTGGAGG TAGTGACGGT TTACCATCAG CTTCCTGATG TGTATGAGAA CAGCACATGC 
AACGGCTTTC AGCTGGACGT CTCTATCGCT GAGACTAATG AAAAAACCTC ATCGGATGTA 
GAAAAGTCCT TCAGACTCAA TGTCAATGTG AGGGCTCTAG AAGAACGGCA AGTGAGGATG 
GTGATTCTAG ACATCGGCCT GCCTACAGGC TTTGAACCCG AAAACTCTGA TTTAGAGTTG 
CTCACAAATT CAGTGGATCG CTACATCAAC AACTTCCAAG TGGTGGACAA CCTGAGCGAC 
AGAGGATCCC TCATAATTCA CCTGTTCAAG GTGTCCAACA AAGAGGCAGA AATGATCTCA 
TTCAGACTCA ATCAGAAATT TAAAGTGGGG CTCCTTCAGC CTTCCACAGT CAAAGTTTAC 
CAGTACTACA ACAAAGATAA AAGCTGTAGC AGGTTCTACT CTCCTCCTGA AGACAAACAG 
CAGCTGGATC AGATCTGTGA AGGCGACGTG TGCCGCTGCT CGCAGGGAGA CTGTTGTGTA 
ATGAAGGAAG ACTTTGCATT TTTTGGTAAA GAGCAGAGGA ATGCCGCTGT ATGCAACGGA 
TTACATCATG GTGAATATTT CTTCAGGGTA AATTACTGTT GTTTATTATT CCTGTCAATG 
AACAACTTTT CTTCAGCCTA CAGAGTCAAG CTGATCAGTA TCAGCCAGAG TCACTATTAC 
CAGTATGAGA TGGAGATTGT GCAGGTTATC AAAGAAGGGA CGGAGGAAGG TCTGAATGAG 
AAGGAGAGGA GGATGTTCCT CTCTCATGCA AGCTGCAGGA CCAGGCTTAA TTTGAAAGAG 
GGTCAGGAGT ATCTGGTCAT TGGGCCGATC ACTGATGTTT GGAATGCAGG GAGCACAGTG 
TCCAACAAGT ATGTGTATAC GCTTGGGAAA GAAACGTGGG TTGAGCGCTG GCCAGATGAG 
TCTGAATGTG GATCAACACT GCAGAAGAAA TGTGATGAGC TGAAGAGCTT TGCAAAGAAA 
CTGATTGAAG ATGGCTGTCA GGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294664.1
CDS	149..5455
Translation	MLLQLLLWATLLCQICDGKAFPQRHKRTVWRHMRQIEPVKDPSPSFSLLAPAKIRPGTKQNILVEGHNLAQVVEVTVVVYDYPNSQTALSRGSVFLNSDNNYSVLQTLEINPNLLLPDGKKKYVKLVAQFSNIYRAEKVMAVSFRSGHIFIQTDKPIYNPGDKVRFRAFVSDLEFRSSERTISLEIQNPDGIAVHGIGRVKAIDGVFSDIFSLSTVVKEGRWKVVAKFDQGKENVFSREFEVKKYVLPAFNVTLTPKTSHLSLDAEKLEVEITARYLYGAQVKGVAYVLFGIEINGEKKRLTSMKQIDDLNGGSVSLTMEEIKRAYPDINSLLGSTLYVKASVLTSSGSDLVEAEKSGIKIVKSPYMLTIKDTPKYYKPGLPLAMTVIVSNHDGSPTPNIPVKVTFLESPISVHSGTINIHINMPKTHILYGNSQILKVETVDPSLKPEQQAIQEVYVKPYSTFSGHDNFLHVSVGASRVVVGGSVSVQMYIKSSLPNHRALVEHLTYIVLNKGKIIQAGRVNVKGQDVTSVHLLITTEMLPAFRFVAYYVLPWQHRAEVVADSVFVDVEDRCVGSLNVGPVDGEIVSSYSPGSSFKFQVKGDVGAKVSLVAVDNAIFLLSKNRLTQKKVWEVVDQADMGCTAGGGKDNMGVFSDAGLMFHSSTGGSTDSKEDCSSKTKRRRRSAAKLQLRDQLEKAYSDELLQRCCVDGMKEIPMPYSCYRRSLYITEDWSCVLAFLRCCAEYRGEELGVITRPPPTTAPPTIAPPTTSPPTLFLVPHLEYHFPEQPVFFVRDRVLQSMPKLHAEANVVGVRQDSYREVVVEEEEEAASVFEEDDLSDLEDIYVRSKFFESWLWTDIRLPSTAKSDGLAVFPVDTVLPDSITQWGFLAVSASPEKGVCVAEPYNVRVWKPFFIDLRLPHSVSRNEQVEIKAVIHNYKNSTLEVMVILEKTEDMCSVAYTGQHRQQVTVAAGSSKLITYTIIPLKTGELPLQVTAVAASFMGQDAVKKNLRVVVEGIQTLKVRSFVLNPSEKGGSAGQQLIQVKKMQLDAVVPKSVPETFVNVRGDLLADSIDNSIKEDSLAALIRMPGGCVEQNLARITLPLIAAHYLDRSADWEIVGISRREEAIKYIQRGYENQLNYKKSDDSYPPYANEGTSTWITAYVVKVFSMAHSFINVNEKHLCGPLLYLLKNKQGYDGTFQEDNPVYDTSMTGAMRGSEYKASLTAFVLIALAEAQDKVTCQDQEVNIRDKSRQAAGYLTEHFSRLTRPYTAAIACYALAVSNNACVKSMLLKFASPDHSHWPDSNNYFFTLEATGYALLALIKSGHMEEAEAPFRWLNEHRGIGGGYGSTQSTMVVLQALSEYLVKRPPPSDLNLLVQLSVPGRSDVRWTFNPNLAYVARSSRVPLDQEFSVAASGNGKGVLEVVTVYHQLPDVYENSTCNGFQLDVSIAETNEKTSSDVEKSFRLNVNVRALEERQVRMVILDIGLPTGFEPENSDLELLTNSVDRYINNFQVVDNLSDRGSLIIHLFKVSNKEAEMISFRLNQKFKVGLLQPSTVKVYQYYNKDKSCSRFYSPPEDKQQLDQICEGDVCRCSQGDCCVMKEDFAFFGKEQRNAAVCNGLHHGEYFFRVNYCCLLFLSMNNFSSAYRVKLISISQSHYYQYEMEIVQVIKEGTEEGLNEKERRMFLSHASCRTRLNLKEGQEYLVIGPITDVWNAGSTVSNKYVYTLGKETWVERWPDESECGSTLQKKCDELKSFAKKLIEDGCQA

Target ORF information:

RefSeq Version	XM_009296389.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio complement component c3b, tandem duplicate 1 (c3b.1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296389.3

ORF Insert Sequence:

ATGCTTCTTC AGCTGCTGTT ATGGGCGACC CTGCTCTGTC AAATCTGCGA TGGAAAGGCC 
TTCCCTCAAC GACACAAGAG GACGGTTTGG AGACATATGC GACAAATTGA GCCGGTCAAA 
GACCCTTCTC CGTCGTTTTC TCTTCTAGCT CCAGCAAAAA TAAGGCCTGG AACAAAACAG 
AACATCCTGG TGGAGGGACA CAACCTGGCA CAGGTGGTAG AAGTCACTGT TGTGGTTTAC 
GACTATCCAA ACTCCCAAAC TGCCTTGTCA CGGGGCTCAG TTTTCCTCAA TTCAGACAAC 
AACTACAGCG TCCTGCAAAC CCTAGAGATC AACCCAAATT TGCTACTTCC AGATGGAAAA 
AAGAAGTATG TGAAGCTCGT TGCTCAGTTC AGCAACATCT ATCGTGCAGA AAAAGTAATG 
GCGGTGTCGT TCCGCTCCGG ACACATCTTC ATTCAGACCG ACAAACCTAT TTACAACCCT 
GGAGACAAAG TACGTTTCAG GGCTTTTGTG TCTGATTTGG AATTTCGTTC TTCTGAGAGA 
ACCATATCTC TGGAGATTCA GAATCCAGAT GGAATTGCGG TGCATGGTAT TGGGAGAGTT 
AAAGCAATCG ATGGGGTCTT TTCTGACATT TTCTCACTGT CTACTGTAGT CAAAGAGGGC 
AGATGGAAAG TTGTAGCAAA GTTTGATCAG GGAAAAGAGA ATGTTTTCAG CAGAGAGTTT 
GAAGTGAAGA AATATGTGCT TCCAGCATTC AACGTCACAC TTACTCCTAA AACATCCCAC 
CTTAGTCTGG ATGCAGAGAA GTTGGAAGTT GAAATAACAG CTAGGTATCT TTATGGCGCG 
CAAGTGAAGG GTGTTGCGTA TGTGCTGTTT GGTATTGAAA TTAATGGTGA AAAGAAGAGA 
CTCACATCTA TGAAGCAAAT TGATGATCTA AATGGAGGAA GTGTTAGTTT GACTATGGAG 
GAGATAAAGA GAGCTTATCC TGACATAAAC AGTCTTCTGG GGTCTACGTT GTACGTCAAA 
GCATCAGTAT TGACTAGTTC AGGTAGCGAT CTTGTGGAAG CAGAGAAATC CGGCATTAAA 
ATCGTAAAAT CACCCTACAT GCTTACCATT AAGGACACAC CAAAGTATTA TAAACCTGGT 
CTGCCGTTAG CCATGACGGT TATCGTGAGT AACCATGATG GATCTCCAAC TCCTAATATT 
CCTGTTAAAG TCACCTTTCT TGAAAGTCCA ATCAGTGTCC ACAGTGGCAC CATCAACATT 
CACATCAACA TGCCGAAAAC GCACATACTA TATGGCAATT CCCAGATATT GAAAGTGGAG 
ACAGTGGATC CGTCACTGAA ACCAGAACAG CAGGCCATTC AAGAGGTATA CGTCAAGCCA 
TATTCTACTT TTAGCGGTCA CGATAACTTC TTGCACGTCT CAGTGGGAGC AAGTCGAGTG 
GTGGTCGGTG GCAGTGTCAG TGTCCAAATG TACATCAAAT CCTCTTTACC AAACCACAGA 
GCCCTCGTGG AGCATCTCAC ATACATTGTG TTAAATAAGG GGAAAATAAT TCAGGCTGGT 
AGAGTGAATG TCAAAGGCCA GGATGTAACA AGCGTTCATC TGCTGATTAC CACAGAAATG 
CTACCTGCAT TTCGGTTTGT GGCGTACTAC GTTTTGCCAT GGCAGCATAG GGCTGAAGTG 
GTGGCAGACT CCGTGTTTGT TGATGTGGAG GACCGGTGTG TAGGTTCGCT TAATGTTGGA 
CCAGTGGATG GAGAAATAGT CAGTTCTTAC TCACCAGGCA GCAGCTTTAA ATTTCAAGTG 
AAAGGTGATG TTGGAGCAAA GGTCAGCCTG GTAGCTGTGG ATAATGCCAT CTTCTTGTTG 
AGTAAAAACC GCCTGACGCA GAAAAAGGTG TGGGAAGTGG TGGATCAGGC TGATATGGGC 
TGCACCGCAG GAGGAGGCAA GGATAATATG GGTGTTTTCA GTGATGCAGG ACTGATGTTT 
CACTCCAGCA CAGGAGGTTC AACTGACTCC AAAGAAGACT GCTCCAGTAA AACTAAACGG 
CGAAGACGTT CTGCAGCCAA ACTGCAGCTC AGAGATCAGC TCGAGAAGGC CTATTCAGAT 
GAGCTCCTGC AGAGGTGTTG TGTGGATGGC ATGAAGGAGA TTCCCATGCC GTATTCATGC 
TACCGTCGCT CTCTCTACAT CACCGAGGAC TGGAGCTGTG TGTTGGCTTT CCTGCGCTGC 
TGTGCAGAAT ACAGAGGAGA GGAGCTCGGC GTCATTACCA GACCTCCACC AACCACTGCT 
CCACCAACCA TTGCTCCGCC AACCACTTCT CCACCCACCC TCTTTCTCGT ACCACATCTT 
GAATATCATT TTCCGGAACA ACCAGTTTTT TTTGTGAGGG ATAGAGTACT ACAATCCATG 
CCAAAGCTGC ATGCCGAAGC TAATGTCGTC GGAGTTCGTC AGGACAGTTA TCGGGAAGTC 
GTTGTAGAAG AAGAAGAAGA GGCGGCTTCA GTGTTTGAGG AAGATGATTT GTCTGATCTT 
GAGGATATTT ATGTGCGCAG CAAGTTTTTT GAGTCGTGGT TGTGGACCGA TATCAGACTG 
CCTAGCACTG CCAAGTCTGA TGGGTTGGCT GTGTTTCCAG TGGACACCGT TCTTCCAGAC 
AGTATCACAC AGTGGGGGTT TCTAGCTGTC AGTGCCTCAC CTGAGAAAGG TGTCTGCGTA 
GCTGAACCGT ATAACGTCCG CGTGTGGAAG CCGTTCTTTA TCGACCTGCG TTTGCCTCAC 
TCGGTGTCCA GAAATGAACA AGTGGAAATC AAAGCTGTAA TCCACAACTA CAAGAACTCA 
ACACTGGAGG TGATGGTGAT CCTGGAGAAG ACAGAGGACA TGTGTAGTGT GGCCTATACG 
GGACAGCACA GACAGCAGGT TACAGTAGCA GCCGGTTCCT CTAAACTCAT TACATACACC 
ATCATCCCTC TGAAAACAGG GGAGCTCCCT TTACAGGTCA CTGCTGTCGC TGCCTCGTTC 
ATGGGTCAAG ATGCAGTGAA GAAAAATCTA CGCGTAGTAG TTGAAGGCAT TCAGACACTT 
AAAGTGAGGA GCTTTGTGTT AAACCCATCA GAGAAAGGAG GCAGTGCTGG GCAACAGTTG 
ATTCAAGTCA AGAAGATGCA GTTGGATGCT GTTGTGCCAA AATCTGTACC AGAAACATTT 
GTCAATGTCA GAGGCGATTT GCTGGCAGAC AGTATTGATA ACTCCATTAA GGAGGACTCG 
TTGGCAGCAT TGATCCGGAT GCCCGGAGGC TGTGTGGAGC AGAACCTGGC CAGAATCACT 
CTGCCCCTCA TCGCAGCTCA TTACCTGGAC CGCAGTGCAG ACTGGGAGAT CGTGGGAATC 
AGTCGCAGAG AAGAGGCCAT CAAATACATA CAGAGAGGTT ATGAAAATCA ACTTAACTAC 
AAAAAATCAG ACGACTCGTA CCCTCCCTAC GCCAATGAAG GCACCAGCAC ATGGATCACT 
GCGTATGTGG TGAAGGTTTT CTCCATGGCG CATTCATTCA TTAATGTCAA TGAGAAGCAT 
CTGTGTGGCC CTCTGCTCTA TTTACTCAAG AATAAACAGG GTTATGATGG AACTTTTCAA 
GAGGACAATC CCGTCTATGA CACCAGTATG ACGGGAGCTA TGCGGGGATC TGAATACAAA 
GCATCTTTGA CTGCATTTGT GCTCATTGCT TTAGCAGAGG CACAAGATAA AGTTACTTGC 
CAAGATCAAG AAGTCAACAT ACGGGATAAG TCTAGACAGG CAGCAGGGTA TCTGACAGAG 
CATTTCTCCC GGCTGACCAG GCCATATACA GCAGCTATAG CATGCTATGC GCTAGCCGTA 
TCGAACAACG CCTGCGTGAA GAGCATGTTG CTCAAATTTG CCTCACCTGA TCATTCCCAC 
TGGCCTGATT CAAACAATTA TTTTTTCACA CTGGAAGCTA CAGGCTACGC ACTGCTAGCG 
CTGATCAAAA GTGGCCACAT GGAAGAGGCA GAAGCTCCAT TTCGATGGTT GAATGAGCAC 
CGTGGCATTG GTGGAGGATA CGGCTCTACT CAGTCCACCA TGGTGGTTCT TCAGGCCCTG 
TCTGAGTATC TAGTGAAGAG GCCTCCTCCG TCAGACCTCA ATTTGTTGGT TCAGCTCAGC 
GTTCCTGGTC GGAGTGATGT TCGCTGGACC TTCAATCCCA ACTTGGCTTA TGTGGCCCGT 
TCTTCACGAG TCCCTCTTGA TCAGGAATTC TCAGTGGCGG CTTCTGGGAA CGGCAAGGGT 
GTTTTGGAGG TAGTGACGGT TTACCATCAG CTTCCTGATG TGTATGAGAA CAGCACATGC 
AACGGCTTTC AGCTGGACGT CTCTATCGCT GAGACTAATG AAAAAACCTC ATCGGATGTA 
GAAAAGTCCT TCAGACTCAA TGTCAATGTG AGGGCTCTAG AAGAACGGCA AGTGAGGATG 
GTGATTCTAG ACATCGGCCT GCCTACAGGC TTTGAACCCG AAAACTCTGA TTTAGAGTTG 
CTCACAAATT CAGTGGATCG CTACATCAAC AACTTCCAAG TGGTGGACAA CCTGAGCGAC 
AGAGGATCCC TCATAATTCA CCTGTTCAAG GTGTCCAACA AAGAGGCAGA AATGATCTCA 
TTCAGACTCA ATCAGAAATT TAAAGTGGGG CTCCTTCAGC CTTCCACAGT CAAAGTTTAC 
CAGTACTACA ACAAAGATAA AAGCTGTAGC AGGTTCTACT CTCCTCCTGA AGACAAACAG 
CAGCTGGATC AGATCTGTGA AGGCGACGTG TGCCGCTGCT CGCAGGGAGA CTGTTGTGTA 
ATGAAGGAAG ACTTTGCATT TTTTGGTAAA GAGCAGAGGA ATGCCGCTGT ATGCAACGGA 
TTACATCATG GTGAATATTT CTTCAGGGTA AATTACTGTT GTTTATTATT CCTGTCAATG 
AACAACTTTT CTTCAGCCTA CAGAGTCAAG CTGATCAGTA TCAGCCAGAG TCACTATTAC 
CAGTATGAGA TGGAGATTGT GCAGGTTATC AAAGAAGGGA CGGAGGAAGG TCTGAATGAG 
AAGGAGAGGA GGATGTTCCT CTCTCATGCA AGCTGCAGGA CCAGGCTTAA TTTGAAAGAG 
GGTCAGGAGT ATCTGGTCAT TGGGCCGATC ACTGATGTTT GGAATGCAGG GAGCACAGTG 
TCCAACAAGT ATGTGTATAC GCTTGGGAAA GAAACGTGGG TTGAGCGCTG GCCAGATGAG 
TCTGAATGTG GATCAACACT GCAGAAGAAA TGTGATGAGC TGAAGAGCTT TGCAAAGAAA 
CTGATTGAAG ATGGCTGTCA GGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

C3B.1 ( XM_009296389.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009294664.1 Danio rerio complement component c3b, tandem duplicate 1 (c3b.1), transcript variant X1, mRNA.

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CloneID	ODa44129
Clone ID Related Accession (Same CDS sequence)	XM_009296389.3 , XM_009296389.2
Accession Version	XM_009296389.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5307bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	complement component c3b, tandem duplicate 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007133.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296389.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCTTCTTC AGCTGCTGTT ATGGGCGACC CTGCTCTGTC AAATCTGCGA TGGAAAGGCC 
TTCCCTCAAC GACACAAGAG GACGGTTTGG AGACATATGC GACAAATTGA GCCGGTCAAA 
GACCCTTCTC CGTCGTTTTC TCTTCTAGCT CCAGCAAAAA TAAGGCCTGG AACAAAACAG 
AACATCCTGG TGGAGGGACA CAACCTGGCA CAGGTGGTAG AAGTCACTGT TGTGGTTTAC 
GACTATCCAA ACTCCCAAAC TGCCTTGTCA CGGGGCTCAG TTTTCCTCAA TTCAGACAAC 
AACTACAGCG TCCTGCAAAC CCTAGAGATC AACCCAAATT TGCTACTTCC AGATGGAAAA 
AAGAAGTATG TGAAGCTCGT TGCTCAGTTC AGCAACATCT ATCGTGCAGA AAAAGTAATG 
GCGGTGTCGT TCCGCTCCGG ACACATCTTC ATTCAGACCG ACAAACCTAT TTACAACCCT 
GGAGACAAAG TACGTTTCAG GGCTTTTGTG TCTGATTTGG AATTTCGTTC TTCTGAGAGA 
ACCATATCTC TGGAGATTCA GAATCCAGAT GGAATTGCGG TGCATGGTAT TGGGAGAGTT 
AAAGCAATCG ATGGGGTCTT TTCTGACATT TTCTCACTGT CTACTGTAGT CAAAGAGGGC 
AGATGGAAAG TTGTAGCAAA GTTTGATCAG GGAAAAGAGA ATGTTTTCAG CAGAGAGTTT 
GAAGTGAAGA AATATGTGCT TCCAGCATTC AACGTCACAC TTACTCCTAA AACATCCCAC 
CTTAGTCTGG ATGCAGAGAA GTTGGAAGTT GAAATAACAG CTAGGTATCT TTATGGCGCG 
CAAGTGAAGG GTGTTGCGTA TGTGCTGTTT GGTATTGAAA TTAATGGTGA AAAGAAGAGA 
CTCACATCTA TGAAGCAAAT TGATGATCTA AATGGAGGAA GTGTTAGTTT GACTATGGAG 
GAGATAAAGA GAGCTTATCC TGACATAAAC AGTCTTCTGG GGTCTACGTT GTACGTCAAA 
GCATCAGTAT TGACTAGTTC AGGTAGCGAT CTTGTGGAAG CAGAGAAATC CGGCATTAAA 
ATCGTAAAAT CACCCTACAT GCTTACCATT AAGGACACAC CAAAGTATTA TAAACCTGGT 
CTGCCGTTAG CCATGACGGT TATCGTGAGT AACCATGATG GATCTCCAAC TCCTAATATT 
CCTGTTAAAG TCACCTTTCT TGAAAGTCCA ATCAGTGTCC ACAGTGGCAC CATCAACATT 
CACATCAACA TGCCGAAAAC GCACATACTA TATGGCAATT CCCAGATATT GAAAGTGGAG 
ACAGTGGATC CGTCACTGAA ACCAGAACAG CAGGCCATTC AAGAGGTATA CGTCAAGCCA 
TATTCTACTT TTAGCGGTCA CGATAACTTC TTGCACGTCT CAGTGGGAGC AAGTCGAGTG 
GTGGTCGGTG GCAGTGTCAG TGTCCAAATG TACATCAAAT CCTCTTTACC AAACCACAGA 
GCCCTCGTGG AGCATCTCAC ATACATTGTG TTAAATAAGG GGAAAATAAT TCAGGCTGGT 
AGAGTGAATG TCAAAGGCCA GGATGTAACA AGCGTTCATC TGCTGATTAC CACAGAAATG 
CTACCTGCAT TTCGGTTTGT GGCGTACTAC GTTTTGCCAT GGCAGCATAG GGCTGAAGTG 
GTGGCAGACT CCGTGTTTGT TGATGTGGAG GACCGGTGTG TAGGTTCGCT TAATGTTGGA 
CCAGTGGATG GAGAAATAGT CAGTTCTTAC TCACCAGGCA GCAGCTTTAA ATTTCAAGTG 
AAAGGTGATG TTGGAGCAAA GGTCAGCCTG GTAGCTGTGG ATAATGCCAT CTTCTTGTTG 
AGTAAAAACC GCCTGACGCA GAAAAAGGTG TGGGAAGTGG TGGATCAGGC TGATATGGGC 
TGCACCGCAG GAGGAGGCAA GGATAATATG GGTGTTTTCA GTGATGCAGG ACTGATGTTT 
CACTCCAGCA CAGGAGGTTC AACTGACTCC AAAGAAGACT GCTCCAGTAA AACTAAACGG 
CGAAGACGTT CTGCAGCCAA ACTGCAGCTC AGAGATCAGC TCGAGAAGGC CTATTCAGAT 
GAGCTCCTGC AGAGGTGTTG TGTGGATGGC ATGAAGGAGA TTCCCATGCC GTATTCATGC 
TACCGTCGCT CTCTCTACAT CACCGAGGAC TGGAGCTGTG TGTTGGCTTT CCTGCGCTGC 
TGTGCAGAAT ACAGAGGAGA GGAGCTCGGC GTCATTACCA GACCTCCACC AACCACTGCT 
CCACCAACCA TTGCTCCGCC AACCACTTCT CCACCCACCC TCTTTCTCGT ACCACATCTT 
GAATATCATT TTCCGGAACA ACCAGTTTTT TTTGTGAGGG ATAGAGTACT ACAATCCATG 
CCAAAGCTGC ATGCCGAAGC TAATGTCGTC GGAGTTCGTC AGGACAGTTA TCGGGAAGTC 
GTTGTAGAAG AAGAAGAAGA GGCGGCTTCA GTGTTTGAGG AAGATGATTT GTCTGATCTT 
GAGGATATTT ATGTGCGCAG CAAGTTTTTT GAGTCGTGGT TGTGGACCGA TATCAGACTG 
CCTAGCACTG CCAAGTCTGA TGGGTTGGCT GTGTTTCCAG TGGACACCGT TCTTCCAGAC 
AGTATCACAC AGTGGGGGTT TCTAGCTGTC AGTGCCTCAC CTGAGAAAGG TGTCTGCGTA 
GCTGAACCGT ATAACGTCCG CGTGTGGAAG CCGTTCTTTA TCGACCTGCG TTTGCCTCAC 
TCGGTGTCCA GAAATGAACA AGTGGAAATC AAAGCTGTAA TCCACAACTA CAAGAACTCA 
ACACTGGAGG TGATGGTGAT CCTGGAGAAG ACAGAGGACA TGTGTAGTGT GGCCTATACG 
GGACAGCACA GACAGCAGGT TACAGTAGCA GCCGGTTCCT CTAAACTCAT TACATACACC 
ATCATCCCTC TGAAAACAGG GGAGCTCCCT TTACAGGTCA CTGCTGTCGC TGCCTCGTTC 
ATGGGTCAAG ATGCAGTGAA GAAAAATCTA CGCGTAGTAG TTGAAGGCAT TCAGACACTT 
AAAGTGAGGA GCTTTGTGTT AAACCCATCA GAGAAAGGAG GCAGTGCTGG GCAACAGTTG 
ATTCAAGTCA AGAAGATGCA GTTGGATGCT GTTGTGCCAA AATCTGTACC AGAAACATTT 
GTCAATGTCA GAGGCGATTT GCTGGCAGAC AGTATTGATA ACTCCATTAA GGAGGACTCG 
TTGGCAGCAT TGATCCGGAT GCCCGGAGGC TGTGTGGAGC AGAACCTGGC CAGAATCACT 
CTGCCCCTCA TCGCAGCTCA TTACCTGGAC CGCAGTGCAG ACTGGGAGAT CGTGGGAATC 
AGTCGCAGAG AAGAGGCCAT CAAATACATA CAGAGAGGTT ATGAAAATCA ACTTAACTAC 
AAAAAATCAG ACGACTCGTA CCCTCCCTAC GCCAATGAAG GCACCAGCAC ATGGATCACT 
GCGTATGTGG TGAAGGTTTT CTCCATGGCG CATTCATTCA TTAATGTCAA TGAGAAGCAT 
CTGTGTGGCC CTCTGCTCTA TTTACTCAAG AATAAACAGG GTTATGATGG AACTTTTCAA 
GAGGACAATC CCGTCTATGA CACCAGTATG ACGGGAGCTA TGCGGGGATC TGAATACAAA 
GCATCTTTGA CTGCATTTGT GCTCATTGCT TTAGCAGAGG CACAAGATAA AGTTACTTGC 
CAAGATCAAG AAGTCAACAT ACGGGATAAG TCTAGACAGG CAGCAGGGTA TCTGACAGAG 
CATTTCTCCC GGCTGACCAG GCCATATACA GCAGCTATAG CATGCTATGC GCTAGCCGTA 
TCGAACAACG CCTGCGTGAA GAGCATGTTG CTCAAATTTG CCTCACCTGA TCATTCCCAC 
TGGCCTGATT CAAACAATTA TTTTTTCACA CTGGAAGCTA CAGGCTACGC ACTGCTAGCG 
CTGATCAAAA GTGGCCACAT GGAAGAGGCA GAAGCTCCAT TTCGATGGTT GAATGAGCAC 
CGTGGCATTG GTGGAGGATA CGGCTCTACT CAGTCCACCA TGGTGGTTCT TCAGGCCCTG 
TCTGAGTATC TAGTGAAGAG GCCTCCTCCG TCAGACCTCA ATTTGTTGGT TCAGCTCAGC 
GTTCCTGGTC GGAGTGATGT TCGCTGGACC TTCAATCCCA ACTTGGCTTA TGTGGCCCGT 
TCTTCACGAG TCCCTCTTGA TCAGGAATTC TCAGTGGCGG CTTCTGGGAA CGGCAAGGGT 
GTTTTGGAGG TAGTGACGGT TTACCATCAG CTTCCTGATG TGTATGAGAA CAGCACATGC 
AACGGCTTTC AGCTGGACGT CTCTATCGCT GAGACTAATG AAAAAACCTC ATCGGATGTA 
GAAAAGTCCT TCAGACTCAA TGTCAATGTG AGGGCTCTAG AAGAACGGCA AGTGAGGATG 
GTGATTCTAG ACATCGGCCT GCCTACAGGC TTTGAACCCG AAAACTCTGA TTTAGAGTTG 
CTCACAAATT CAGTGGATCG CTACATCAAC AACTTCCAAG TGGTGGACAA CCTGAGCGAC 
AGAGGATCCC TCATAATTCA CCTGTTCAAG GTGTCCAACA AAGAGGCAGA AATGATCTCA 
TTCAGACTCA ATCAGAAATT TAAAGTGGGG CTCCTTCAGC CTTCCACAGT CAAAGTTTAC 
CAGTACTACA ACAAAGATAA AAGCTGTAGC AGGTTCTACT CTCCTCCTGA AGACAAACAG 
CAGCTGGATC AGATCTGTGA AGGCGACGTG TGCCGCTGCT CGCAGGGAGA CTGTTGTGTA 
ATGAAGGAAG ACTTTGCATT TTTTGGTAAA GAGCAGAGGA ATGCCGCTGT ATGCAACGGA 
TTACATCATG GTGAATATTT CTTCAGGGTA AATTACTGTT GTTTATTATT CCTGTCAATG 
AACAACTTTT CTTCAGCCTA CAGAGTCAAG CTGATCAGTA TCAGCCAGAG TCACTATTAC 
CAGTATGAGA TGGAGATTGT GCAGGTTATC AAAGAAGGGA CGGAGGAAGG TCTGAATGAG 
AAGGAGAGGA GGATGTTCCT CTCTCATGCA AGCTGCAGGA CCAGGCTTAA TTTGAAAGAG 
GGTCAGGAGT ATCTGGTCAT TGGGCCGATC ACTGATGTTT GGAATGCAGG GAGCACAGTG 
TCCAACAAGT ATGTGTATAC GCTTGGGAAA GAAACGTGGG TTGAGCGCTG GCCAGATGAG 
TCTGAATGTG GATCAACACT GCAGAAGAAA TGTGATGAGC TGAAGAGCTT TGCAAAGAAA 
CTGATTGAAG ATGGCTGTCA GGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009294664.1
CDS	125..5431
Translation	MLLQLLLWATLLCQICDGKAFPQRHKRTVWRHMRQIEPVKDPSPSFSLLAPAKIRPGTKQNILVEGHNLAQVVEVTVVVYDYPNSQTALSRGSVFLNSDNNYSVLQTLEINPNLLLPDGKKKYVKLVAQFSNIYRAEKVMAVSFRSGHIFIQTDKPIYNPGDKVRFRAFVSDLEFRSSERTISLEIQNPDGIAVHGIGRVKAIDGVFSDIFSLSTVVKEGRWKVVAKFDQGKENVFSREFEVKKYVLPAFNVTLTPKTSHLSLDAEKLEVEITARYLYGAQVKGVAYVLFGIEINGEKKRLTSMKQIDDLNGGSVSLTMEEIKRAYPDINSLLGSTLYVKASVLTSSGSDLVEAEKSGIKIVKSPYMLTIKDTPKYYKPGLPLAMTVIVSNHDGSPTPNIPVKVTFLESPISVHSGTINIHINMPKTHILYGNSQILKVETVDPSLKPEQQAIQEVYVKPYSTFSGHDNFLHVSVGASRVVVGGSVSVQMYIKSSLPNHRALVEHLTYIVLNKGKIIQAGRVNVKGQDVTSVHLLITTEMLPAFRFVAYYVLPWQHRAEVVADSVFVDVEDRCVGSLNVGPVDGEIVSSYSPGSSFKFQVKGDVGAKVSLVAVDNAIFLLSKNRLTQKKVWEVVDQADMGCTAGGGKDNMGVFSDAGLMFHSSTGGSTDSKEDCSSKTKRRRRSAAKLQLRDQLEKAYSDELLQRCCVDGMKEIPMPYSCYRRSLYITEDWSCVLAFLRCCAEYRGEELGVITRPPPTTAPPTIAPPTTSPPTLFLVPHLEYHFPEQPVFFVRDRVLQSMPKLHAEANVVGVRQDSYREVVVEEEEEAASVFEEDDLSDLEDIYVRSKFFESWLWTDIRLPSTAKSDGLAVFPVDTVLPDSITQWGFLAVSASPEKGVCVAEPYNVRVWKPFFIDLRLPHSVSRNEQVEIKAVIHNYKNSTLEVMVILEKTEDMCSVAYTGQHRQQVTVAAGSSKLITYTIIPLKTGELPLQVTAVAASFMGQDAVKKNLRVVVEGIQTLKVRSFVLNPSEKGGSAGQQLIQVKKMQLDAVVPKSVPETFVNVRGDLLADSIDNSIKEDSLAALIRMPGGCVEQNLARITLPLIAAHYLDRSADWEIVGISRREEAIKYIQRGYENQLNYKKSDDSYPPYANEGTSTWITAYVVKVFSMAHSFINVNEKHLCGPLLYLLKNKQGYDGTFQEDNPVYDTSMTGAMRGSEYKASLTAFVLIALAEAQDKVTCQDQEVNIRDKSRQAAGYLTEHFSRLTRPYTAAIACYALAVSNNACVKSMLLKFASPDHSHWPDSNNYFFTLEATGYALLALIKSGHMEEAEAPFRWLNEHRGIGGGYGSTQSTMVVLQALSEYLVKRPPPSDLNLLVQLSVPGRSDVRWTFNPNLAYVARSSRVPLDQEFSVAASGNGKGVLEVVTVYHQLPDVYENSTCNGFQLDVSIAETNEKTSSDVEKSFRLNVNVRALEERQVRMVILDIGLPTGFEPENSDLELLTNSVDRYINNFQVVDNLSDRGSLIIHLFKVSNKEAEMISFRLNQKFKVGLLQPSTVKVYQYYNKDKSCSRFYSPPEDKQQLDQICEGDVCRCSQGDCCVMKEDFAFFGKEQRNAAVCNGLHHGEYFFRVNYCCLLFLSMNNFSSAYRVKLISISQSHYYQYEMEIVQVIKEGTEEGLNEKERRMFLSHASCRTRLNLKEGQEYLVIGPITDVWNAGSTVSNKYVYTLGKETWVERWPDESECGSTLQKKCDELKSFAKKLIEDGCQA

Target ORF information:

RefSeq Version	XM_009296389.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio complement component c3b, tandem duplicate 1 (c3b.1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296389.2

ORF Insert Sequence:

ATGCTTCTTC AGCTGCTGTT ATGGGCGACC CTGCTCTGTC AAATCTGCGA TGGAAAGGCC 
TTCCCTCAAC GACACAAGAG GACGGTTTGG AGACATATGC GACAAATTGA GCCGGTCAAA 
GACCCTTCTC CGTCGTTTTC TCTTCTAGCT CCAGCAAAAA TAAGGCCTGG AACAAAACAG 
AACATCCTGG TGGAGGGACA CAACCTGGCA CAGGTGGTAG AAGTCACTGT TGTGGTTTAC 
GACTATCCAA ACTCCCAAAC TGCCTTGTCA CGGGGCTCAG TTTTCCTCAA TTCAGACAAC 
AACTACAGCG TCCTGCAAAC CCTAGAGATC AACCCAAATT TGCTACTTCC AGATGGAAAA 
AAGAAGTATG TGAAGCTCGT TGCTCAGTTC AGCAACATCT ATCGTGCAGA AAAAGTAATG 
GCGGTGTCGT TCCGCTCCGG ACACATCTTC ATTCAGACCG ACAAACCTAT TTACAACCCT 
GGAGACAAAG TACGTTTCAG GGCTTTTGTG TCTGATTTGG AATTTCGTTC TTCTGAGAGA 
ACCATATCTC TGGAGATTCA GAATCCAGAT GGAATTGCGG TGCATGGTAT TGGGAGAGTT 
AAAGCAATCG ATGGGGTCTT TTCTGACATT TTCTCACTGT CTACTGTAGT CAAAGAGGGC 
AGATGGAAAG TTGTAGCAAA GTTTGATCAG GGAAAAGAGA ATGTTTTCAG CAGAGAGTTT 
GAAGTGAAGA AATATGTGCT TCCAGCATTC AACGTCACAC TTACTCCTAA AACATCCCAC 
CTTAGTCTGG ATGCAGAGAA GTTGGAAGTT GAAATAACAG CTAGGTATCT TTATGGCGCG 
CAAGTGAAGG GTGTTGCGTA TGTGCTGTTT GGTATTGAAA TTAATGGTGA AAAGAAGAGA 
CTCACATCTA TGAAGCAAAT TGATGATCTA AATGGAGGAA GTGTTAGTTT GACTATGGAG 
GAGATAAAGA GAGCTTATCC TGACATAAAC AGTCTTCTGG GGTCTACGTT GTACGTCAAA 
GCATCAGTAT TGACTAGTTC AGGTAGCGAT CTTGTGGAAG CAGAGAAATC CGGCATTAAA 
ATCGTAAAAT CACCCTACAT GCTTACCATT AAGGACACAC CAAAGTATTA TAAACCTGGT 
CTGCCGTTAG CCATGACGGT TATCGTGAGT AACCATGATG GATCTCCAAC TCCTAATATT 
CCTGTTAAAG TCACCTTTCT TGAAAGTCCA ATCAGTGTCC ACAGTGGCAC CATCAACATT 
CACATCAACA TGCCGAAAAC GCACATACTA TATGGCAATT CCCAGATATT GAAAGTGGAG 
ACAGTGGATC CGTCACTGAA ACCAGAACAG CAGGCCATTC AAGAGGTATA CGTCAAGCCA 
TATTCTACTT TTAGCGGTCA CGATAACTTC TTGCACGTCT CAGTGGGAGC AAGTCGAGTG 
GTGGTCGGTG GCAGTGTCAG TGTCCAAATG TACATCAAAT CCTCTTTACC AAACCACAGA 
GCCCTCGTGG AGCATCTCAC ATACATTGTG TTAAATAAGG GGAAAATAAT TCAGGCTGGT 
AGAGTGAATG TCAAAGGCCA GGATGTAACA AGCGTTCATC TGCTGATTAC CACAGAAATG 
CTACCTGCAT TTCGGTTTGT GGCGTACTAC GTTTTGCCAT GGCAGCATAG GGCTGAAGTG 
GTGGCAGACT CCGTGTTTGT TGATGTGGAG GACCGGTGTG TAGGTTCGCT TAATGTTGGA 
CCAGTGGATG GAGAAATAGT CAGTTCTTAC TCACCAGGCA GCAGCTTTAA ATTTCAAGTG 
AAAGGTGATG TTGGAGCAAA GGTCAGCCTG GTAGCTGTGG ATAATGCCAT CTTCTTGTTG 
AGTAAAAACC GCCTGACGCA GAAAAAGGTG TGGGAAGTGG TGGATCAGGC TGATATGGGC 
TGCACCGCAG GAGGAGGCAA GGATAATATG GGTGTTTTCA GTGATGCAGG ACTGATGTTT 
CACTCCAGCA CAGGAGGTTC AACTGACTCC AAAGAAGACT GCTCCAGTAA AACTAAACGG 
CGAAGACGTT CTGCAGCCAA ACTGCAGCTC AGAGATCAGC TCGAGAAGGC CTATTCAGAT 
GAGCTCCTGC AGAGGTGTTG TGTGGATGGC ATGAAGGAGA TTCCCATGCC GTATTCATGC 
TACCGTCGCT CTCTCTACAT CACCGAGGAC TGGAGCTGTG TGTTGGCTTT CCTGCGCTGC 
TGTGCAGAAT ACAGAGGAGA GGAGCTCGGC GTCATTACCA GACCTCCACC AACCACTGCT 
CCACCAACCA TTGCTCCGCC AACCACTTCT CCACCCACCC TCTTTCTCGT ACCACATCTT 
GAATATCATT TTCCGGAACA ACCAGTTTTT TTTGTGAGGG ATAGAGTACT ACAATCCATG 
CCAAAGCTGC ATGCCGAAGC TAATGTCGTC GGAGTTCGTC AGGACAGTTA TCGGGAAGTC 
GTTGTAGAAG AAGAAGAAGA GGCGGCTTCA GTGTTTGAGG AAGATGATTT GTCTGATCTT 
GAGGATATTT ATGTGCGCAG CAAGTTTTTT GAGTCGTGGT TGTGGACCGA TATCAGACTG 
CCTAGCACTG CCAAGTCTGA TGGGTTGGCT GTGTTTCCAG TGGACACCGT TCTTCCAGAC 
AGTATCACAC AGTGGGGGTT TCTAGCTGTC AGTGCCTCAC CTGAGAAAGG TGTCTGCGTA 
GCTGAACCGT ATAACGTCCG CGTGTGGAAG CCGTTCTTTA TCGACCTGCG TTTGCCTCAC 
TCGGTGTCCA GAAATGAACA AGTGGAAATC AAAGCTGTAA TCCACAACTA CAAGAACTCA 
ACACTGGAGG TGATGGTGAT CCTGGAGAAG ACAGAGGACA TGTGTAGTGT GGCCTATACG 
GGACAGCACA GACAGCAGGT TACAGTAGCA GCCGGTTCCT CTAAACTCAT TACATACACC 
ATCATCCCTC TGAAAACAGG GGAGCTCCCT TTACAGGTCA CTGCTGTCGC TGCCTCGTTC 
ATGGGTCAAG ATGCAGTGAA GAAAAATCTA CGCGTAGTAG TTGAAGGCAT TCAGACACTT 
AAAGTGAGGA GCTTTGTGTT AAACCCATCA GAGAAAGGAG GCAGTGCTGG GCAACAGTTG 
ATTCAAGTCA AGAAGATGCA GTTGGATGCT GTTGTGCCAA AATCTGTACC AGAAACATTT 
GTCAATGTCA GAGGCGATTT GCTGGCAGAC AGTATTGATA ACTCCATTAA GGAGGACTCG 
TTGGCAGCAT TGATCCGGAT GCCCGGAGGC TGTGTGGAGC AGAACCTGGC CAGAATCACT 
CTGCCCCTCA TCGCAGCTCA TTACCTGGAC CGCAGTGCAG ACTGGGAGAT CGTGGGAATC 
AGTCGCAGAG AAGAGGCCAT CAAATACATA CAGAGAGGTT ATGAAAATCA ACTTAACTAC 
AAAAAATCAG ACGACTCGTA CCCTCCCTAC GCCAATGAAG GCACCAGCAC ATGGATCACT 
GCGTATGTGG TGAAGGTTTT CTCCATGGCG CATTCATTCA TTAATGTCAA TGAGAAGCAT 
CTGTGTGGCC CTCTGCTCTA TTTACTCAAG AATAAACAGG GTTATGATGG AACTTTTCAA 
GAGGACAATC CCGTCTATGA CACCAGTATG ACGGGAGCTA TGCGGGGATC TGAATACAAA 
GCATCTTTGA CTGCATTTGT GCTCATTGCT TTAGCAGAGG CACAAGATAA AGTTACTTGC 
CAAGATCAAG AAGTCAACAT ACGGGATAAG TCTAGACAGG CAGCAGGGTA TCTGACAGAG 
CATTTCTCCC GGCTGACCAG GCCATATACA GCAGCTATAG CATGCTATGC GCTAGCCGTA 
TCGAACAACG CCTGCGTGAA GAGCATGTTG CTCAAATTTG CCTCACCTGA TCATTCCCAC 
TGGCCTGATT CAAACAATTA TTTTTTCACA CTGGAAGCTA CAGGCTACGC ACTGCTAGCG 
CTGATCAAAA GTGGCCACAT GGAAGAGGCA GAAGCTCCAT TTCGATGGTT GAATGAGCAC 
CGTGGCATTG GTGGAGGATA CGGCTCTACT CAGTCCACCA TGGTGGTTCT TCAGGCCCTG 
TCTGAGTATC TAGTGAAGAG GCCTCCTCCG TCAGACCTCA ATTTGTTGGT TCAGCTCAGC 
GTTCCTGGTC GGAGTGATGT TCGCTGGACC TTCAATCCCA ACTTGGCTTA TGTGGCCCGT 
TCTTCACGAG TCCCTCTTGA TCAGGAATTC TCAGTGGCGG CTTCTGGGAA CGGCAAGGGT 
GTTTTGGAGG TAGTGACGGT TTACCATCAG CTTCCTGATG TGTATGAGAA CAGCACATGC 
AACGGCTTTC AGCTGGACGT CTCTATCGCT GAGACTAATG AAAAAACCTC ATCGGATGTA 
GAAAAGTCCT TCAGACTCAA TGTCAATGTG AGGGCTCTAG AAGAACGGCA AGTGAGGATG 
GTGATTCTAG ACATCGGCCT GCCTACAGGC TTTGAACCCG AAAACTCTGA TTTAGAGTTG 
CTCACAAATT CAGTGGATCG CTACATCAAC AACTTCCAAG TGGTGGACAA CCTGAGCGAC 
AGAGGATCCC TCATAATTCA CCTGTTCAAG GTGTCCAACA AAGAGGCAGA AATGATCTCA 
TTCAGACTCA ATCAGAAATT TAAAGTGGGG CTCCTTCAGC CTTCCACAGT CAAAGTTTAC 
CAGTACTACA ACAAAGATAA AAGCTGTAGC AGGTTCTACT CTCCTCCTGA AGACAAACAG 
CAGCTGGATC AGATCTGTGA AGGCGACGTG TGCCGCTGCT CGCAGGGAGA CTGTTGTGTA 
ATGAAGGAAG ACTTTGCATT TTTTGGTAAA GAGCAGAGGA ATGCCGCTGT ATGCAACGGA 
TTACATCATG GTGAATATTT CTTCAGGGTA AATTACTGTT GTTTATTATT CCTGTCAATG 
AACAACTTTT CTTCAGCCTA CAGAGTCAAG CTGATCAGTA TCAGCCAGAG TCACTATTAC 
CAGTATGAGA TGGAGATTGT GCAGGTTATC AAAGAAGGGA CGGAGGAAGG TCTGAATGAG 
AAGGAGAGGA GGATGTTCCT CTCTCATGCA AGCTGCAGGA CCAGGCTTAA TTTGAAAGAG 
GGTCAGGAGT ATCTGGTCAT TGGGCCGATC ACTGATGTTT GGAATGCAGG GAGCACAGTG 
TCCAACAAGT ATGTGTATAC GCTTGGGAAA GAAACGTGGG TTGAGCGCTG GCCAGATGAG 
TCTGAATGTG GATCAACACT GCAGAAGAAA TGTGATGAGC TGAAGAGCTT TGCAAAGAAA 
CTGATTGAAG ATGGCTGTCA GGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Exposure to mercuric chloride induces developmental damage, oxidative stress and immunotoxicity in zebrafish embryos-larvae.
Aquatic toxicology (Amsterdam, Netherlands)18176-85(2016 Dec)
Zhang QF,Li YW,Liu ZH,Chen QL

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

The evolution and appearance of C3 duplications in fish originate an exclusive teleost c3 gene form with anti-inflammatory activity.
PloS one9(6)e99673(2014)
Forn-Cun? G,Reis ES,Dios S,Posada D,Lambris JD,Figueras A,Novoa B

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C3B.1 cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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