tubgcp3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	tubgcp3
Entrez Gene ID	563517
Full Name	tubulin, gamma complex associated protein 3
Synonyms	GCP3,SPC98P,si:dz75p05.1,wu:fc09h08
General protein information	Preferred Names tubulin, gamma complex associated protein 3 Names gamma-tubulin complex component 3 spindle pole body
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005170995.3	XP_005171052.1	gamma-tubulin complex component 3 isoform X1
NM_001004513.1	NP_001004513.1	gamma-tubulin complex component 3
XM_005170995.4	XP_005171052.1	gamma-tubulin complex component 3 isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	TUBGCP3	NP_006313.1
Canis lupus familiaris (dog)	TUBGCP3	XP_534189.2
Gallus gallus (chicken)	TUBGCP3	XP_416949.2
Danio rerio (zebrafish)	tubgcp3	NP_001004513.1
Mus musculus (house mouse)	Tubgcp3	NP_932148.1
Arabidopsis thaliana (thale cress)	SPC98	NP_196286.1
Pan troglodytes (chimpanzee)	TUBGCP3	XP_001142636.2
Drosophila melanogaster (fruit fly)	l(1)dd4	NP_524919.2
Bos taurus (cattle)	TUBGCP3	NP_001179561.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP003865	XP_310424.4
Xenopus tropicalis (tropical clawed frog)	tubgcp3	NP_001116269.1
Oryza sativa (rice)	Os09g0446200	NP_001063308.2

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Tissue-restricted expression of Nrf2 and its target genes in zebrafish with gene-specific variations in the induction profiles.
Nakajima H, Nakajima-Takagi Y, Tsujita T, Akiyama S, Wakasa T, Mukaigasa K, Kaneko H, Tamaru Y, Yamamoto M, Kobayashi M
PloS one6(10)e26884(2011)

Transcriptomic analyses reveal novel genes with sexually dimorphic expression in the zebrafish gonad and brain.
Sreenivasan R, Cai M, Bartfai R, Wang X, Christoffels A, Orban L
PloS one3(3)e1791(2008 Mar)

Soluble tubulin complexes, gamma-tubulin, and their changing distribution in the zebrafish (Danio rerio) ovary, oocyte and embryo.
Liu J, Lessman CA
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology147(1)56-73(2007 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

These findings, suggest that soluble gamma-tubulin-associated protein complexes may be involved in regulating tubulin dynamics during zebrafish oogenesis and embryogenesis.[gamma tubulin]
Title: Soluble tubulin complexes, gamma-tubulin, and their changing distribution in the zebrafish (Danio rerio) ovary, oocyte and embryo.

The following tubgcp3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the tubgcp3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa34498	NM_001004513.1 Latest version!	Danio rerio tubulin, gamma complex associated protein 3 (tubgcp3), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa06123	XM_005170995.4 Latest version!	Danio rerio tubulin, gamma complex associated protein 3 (tubgcp3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
ODa06123	XM_005170995.3	Danio rerio tubulin, gamma complex associated protein 3 (tubgcp3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

tubgcp3 ( NM_001004513.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001004513.1 Danio rerio tubulin, gamma complex associated protein 3 (tubgcp3), mRNA.

Price & Availability↑

CloneID	ODa34498
Clone ID Related Accession (Same CDS sequence)	NM_001004513.1
Accession Version	NM_001004513.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2700bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	gamma-tubulin complex component 3
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX465849.10 and BX470136.8. On May 18, 2009 this sequence version replaced XM_686878.3. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGCGACGC TTGATCAGAA ATCCCCTAAC GTGCTTCTGC AGAGCCTGTG CTGTAAGATC 
ACGGGCAAGA GCGAAGCTGA CGTAGCCCAT CAGTTCCAGT ACGCTGTGCG GGTCATTGGA 
AGTAATTACG CTCCCACAAT TGAACGGGAC GAGTTTTTGG TGTCTGAGAA AATTAAGAAA 
GAATTGTTGA AACAAAGGCG GGAAGCAGAT GCAGCCCTTT TCTCTGAGTT GCACAGAAAG 
CTTCAGACCC AGGGTGTATT GAAACACAGA TGGTCCATCT TATACCTCCT GCTCAGCCTC 
TGTGAGGACC CAAGGAAACC ATCCGGCCGG GTTCCTGTGT GTAGTTATGG AGCTCTGCTT 
GCTCAGGCTT TGCCCCGTGA TGTTCACTCC ACACCGTTCT ACTATGCCCG TCCTCAGAGT 
CTCCCACTCA GCTACCCAGA GCGCTCCGCC ACAGCTCATA ACTCCAGCAT GGCTACCAGT 
GGAATCAGCA GTATGGGGGT GTTTTCTATT AATGGGCCTG GACCGACCCC ACCATCTTTG 
CTTACTGGGT CATCTATGAG AGGGTCTCGT TTAGCCTGGA CTCTGCCAGT GTCCAGTTCT 
CCGTCTGGGG CAGCAGCTGC TCCTCGTCCC CCCAGCGGAC CCTCCTCCAG TCCAGCTCCC 
AAACCACTGC AAAGACCCAG GCGGGACGGA GATGGCAGTG CTGATATTGG GGAAGCTGCT 
CTCGTCCGAG ACATTCTTTA TGTTTTCCAG GGAATCGACG GAAAGTTCAT CAAGATGTGC 
AGCCCGGAAA ACTGCTACAA AATTGATTCA AAGGTGCCAC TGTGTAAGTC TTTGAGAGAC 
ACTAGCAGCA GGCTGGCCGA GCTCGGCTGG CTCCACAATA AAATTAGAAA ATATACAGAC 
TCACACAGCT TGGATCGAGC CTTTGGTCTT GTTGGGCAGA GTTTTTGTGC TGCTCTGCAT 
CAGGAACTGA AAGAGTACTA CAGATTGCTC TCTGTACTTC ATGCACAGCT CCAGGTGGAG 
GACGATCAGG GTGTAAATAT GAGTGTTGAC AGCAGTTTGA CTCTGAGGAG ACTTCTGGTG 
TGGACCTACG ACCCCAAGGT TCGACTAAAA ACACTGGCCG CCCTGGTGGA CTACTGCCAA 
GGGCGTAAGG GTGGAGAGTT GGCCACTGCT GTACATGCGT ATGGAAAGAC GGGTGACCCC 
AACATGAGAG CACTGATCCA GCACATCCTT GGTCTGGTGT CTCATCCGAT TCTCAACTTC 
TTATACCGCT GGATTTATGA TGGAGAGCTG GAGGATACGT ACCATGAGTT TTTTGTAGCC 
TCGGACCCAA CAGTGAAGAC GGACAGACTC TGGCATGACA AATACTCTCT AAGGAAGAGT 
ATGATCCCAT CCTTCATCAC CATGGACCAG GCCAGGAAGG TTCTTCTTAT TGGCAAATCC 
ATCAACTTCC TGCATCAGGT TTGTCATGAC CGGACACCAC CGGGAAAGAT CATGCCTGCC 
TCCAAATCTA AAGACTCTCC CAAAGATGCT GCAGAGTTGT TCACTGATCT GGAAGGGACC 
TTTCAGAGTA AGATTGATGC TGCGTATTTT GAGACCAGTA AGTATCTTCT AGACGTGCTG 
AATAAGAACT ACCAGCTTCT GGAGCATCTT CAGGCCATGA GGAGATACCT GCTGCTGGGC 
CAGGGAGACT TTATCCGGCA CCTCATGGAC CTCCTTAAGC CAGAGTTAGC CAGGCCTGCC 
ACCACTCTTT ACCAGCACAA TCTAACCGGA ATACTTGAGA CGGCCGTCAG AGCAACCAAT 
GCCCAATTTG ACAGCCCTGA GATACTGAAG AGGCTTGATG TCCGGCTGCT GGAGGTTTCT 
CCTGGAGACA CTGGCTGGGA CGTCTTCAGT TTGGACTATC ATGTTGAAGG ACCCATTGCC 
ACAGTGTTCA CCCGTGAGTG TATGAGCCAC TACTTGCGTG TCTTCAACTT CCTGTGGAGG 
GCTAAAAGAA TGGAGTACAT CCTCACAGAC ATCTGGAAGG GTCAGATGTG TAACGCCAAG 
CTGCTGAAGA GCATGCCGGA GCTTTCTGGT GTGCTGCACC AGTGTCACGT TCTTGCTTCT 
GAAATGGTTC ACTTCATTCA TCAGATGCAG TATTACATCA CATTTGAGGT GTTGGAATGC 
TCTTGGGATG AGTTGTGGAA TAAAGTCCAG CAGGCTCAGG ATCTGGATCA CATTATAGCA 
GCTCATGACG TTTTCCTGGA CACCATCATT TCTCGCTGCC TGCTGGATGT GAACAACCGG 
TCTCTGTTGA ATCAGTTGCG CGCAGTGTTT GATCAGATCA TCGAGTTCCA GAATGCTCAG 
GACACACTGT ACCGCTCTGC TCTAGAAGAA CTGCAGCTAC GCATACAATT CGAGGACAAG 
AAGAGACAGA GAGAAGAGAT GGCCGAGTGG GGTGTAACAG CAGAGCAGGA GGCCGAGGAG 
AACAGACGCG TGCAGGAGTT TAAAGACACC ATACCAAAAA TGTGCTCTCA GCTCCGATTG 
CTCACACACT TCTATCAGGG CATAGTGCAG GAGTTCCTGC GGCTGCTAAT GACCTGCTCT 
GACGAGAGTC TCCAGTTCCT CAGTTTTCGT CTGGATTTCA ATGAACATTA CAAGGCCCGT 
GACCCCCGCC TGCGGCCCTC GCTCGGGGCC ACCAGAGGCA GACGGAGCTC CAACATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001004513.1
CDS	1..2700
Translation	MATLDQKSPNVLLQSLCCKITGKSEADVAHQFQYAVRVIGSNYAPTIERDEFLVSEKIKKELLKQRREADAALFSELHRKLQTQGVLKHRWSILYLLLSLCEDPRKPSGRVPVCSYGALLAQALPRDVHSTPFYYARPQSLPLSYPERSATAHNSSMATSGISSMGVFSINGPGPTPPSLLTGSSMRGSRLAWTLPVSSSPSGAAAAPRPPSGPSSSPAPKPLQRPRRDGDGSADIGEAALVRDILYVFQGIDGKFIKMCSPENCYKIDSKVPLCKSLRDTSSRLAELGWLHNKIRKYTDSHSLDRAFGLVGQSFCAALHQELKEYYRLLSVLHAQLQVEDDQGVNMSVDSSLTLRRLLVWTYDPKVRLKTLAALVDYCQGRKGGELATAVHAYGKTGDPNMRALIQHILGLVSHPILNFLYRWIYDGELEDTYHEFFVASDPTVKTDRLWHDKYSLRKSMIPSFITMDQARKVLLIGKSINFLHQVCHDRTPPGKIMPASKSKDSPKDAAELFTDLEGTFQSKIDAAYFETSKYLLDVLNKNYQLLEHLQAMRRYLLLGQGDFIRHLMDLLKPELARPATTLYQHNLTGILETAVRATNAQFDSPEILKRLDVRLLEVSPGDTGWDVFSLDYHVEGPIATVFTRECMSHYLRVFNFLWRAKRMEYILTDIWKGQMCNAKLLKSMPELSGVLHQCHVLASEMVHFIHQMQYYITFEVLECSWDELWNKVQQAQDLDHIIAAHDVFLDTIISRCLLDVNNRSLLNQLRAVFDQIIEFQNAQDTLYRSALEELQLRIQFEDKKRQREEMAEWGVTAEQEAEENRRVQEFKDTIPKMCSQLRLLTHFYQGIVQEFLRLLMTCSDESLQFLSFRLDFNEHYKARDPRLRPSLGATRGRRSSNI

Target ORF information:

RefSeq Version	NM_001004513.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tubulin, gamma complex associated protein 3 (tubgcp3), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001004513.1

ORF Insert Sequence:

ATGGCGACGC TTGATCAGAA ATCCCCTAAC GTGCTTCTGC AGAGCCTGTG CTGTAAGATC 
ACGGGCAAGA GCGAAGCTGA CGTAGCCCAT CAGTTCCAGT ACGCTGTGCG GGTCATTGGA 
AGTAATTACG CTCCCACAAT TGAACGGGAC GAGTTTTTGG TGTCTGAGAA AATTAAGAAA 
GAATTGTTGA AACAAAGGCG GGAAGCAGAT GCAGCCCTTT TCTCTGAGTT GCACAGAAAG 
CTTCAGACCC AGGGTGTATT GAAACACAGA TGGTCCATCT TATACCTCCT GCTCAGCCTC 
TGTGAGGACC CAAGGAAACC ATCCGGCCGG GTTCCTGTGT GTAGTTATGG AGCTCTGCTT 
GCTCAGGCTT TGCCCCGTGA TGTTCACTCC ACACCGTTCT ACTATGCCCG TCCTCAGAGT 
CTCCCACTCA GCTACCCAGA GCGCTCCGCC ACAGCTCATA ACTCCAGCAT GGCTACCAGT 
GGAATCAGCA GTATGGGGGT GTTTTCTATT AATGGGCCTG GACCGACCCC ACCATCTTTG 
CTTACTGGGT CATCTATGAG AGGGTCTCGT TTAGCCTGGA CTCTGCCAGT GTCCAGTTCT 
CCGTCTGGGG CAGCAGCTGC TCCTCGTCCC CCCAGCGGAC CCTCCTCCAG TCCAGCTCCC 
AAACCACTGC AAAGACCCAG GCGGGACGGA GATGGCAGTG CTGATATTGG GGAAGCTGCT 
CTCGTCCGAG ACATTCTTTA TGTTTTCCAG GGAATCGACG GAAAGTTCAT CAAGATGTGC 
AGCCCGGAAA ACTGCTACAA AATTGATTCA AAGGTGCCAC TGTGTAAGTC TTTGAGAGAC 
ACTAGCAGCA GGCTGGCCGA GCTCGGCTGG CTCCACAATA AAATTAGAAA ATATACAGAC 
TCACACAGCT TGGATCGAGC CTTTGGTCTT GTTGGGCAGA GTTTTTGTGC TGCTCTGCAT 
CAGGAACTGA AAGAGTACTA CAGATTGCTC TCTGTACTTC ATGCACAGCT CCAGGTGGAG 
GACGATCAGG GTGTAAATAT GAGTGTTGAC AGCAGTTTGA CTCTGAGGAG ACTTCTGGTG 
TGGACCTACG ACCCCAAGGT TCGACTAAAA ACACTGGCCG CCCTGGTGGA CTACTGCCAA 
GGGCGTAAGG GTGGAGAGTT GGCCACTGCT GTACATGCGT ATGGAAAGAC GGGTGACCCC 
AACATGAGAG CACTGATCCA GCACATCCTT GGTCTGGTGT CTCATCCGAT TCTCAACTTC 
TTATACCGCT GGATTTATGA TGGAGAGCTG GAGGATACGT ACCATGAGTT TTTTGTAGCC 
TCGGACCCAA CAGTGAAGAC GGACAGACTC TGGCATGACA AATACTCTCT AAGGAAGAGT 
ATGATCCCAT CCTTCATCAC CATGGACCAG GCCAGGAAGG TTCTTCTTAT TGGCAAATCC 
ATCAACTTCC TGCATCAGGT TTGTCATGAC CGGACACCAC CGGGAAAGAT CATGCCTGCC 
TCCAAATCTA AAGACTCTCC CAAAGATGCT GCAGAGTTGT TCACTGATCT GGAAGGGACC 
TTTCAGAGTA AGATTGATGC TGCGTATTTT GAGACCAGTA AGTATCTTCT AGACGTGCTG 
AATAAGAACT ACCAGCTTCT GGAGCATCTT CAGGCCATGA GGAGATACCT GCTGCTGGGC 
CAGGGAGACT TTATCCGGCA CCTCATGGAC CTCCTTAAGC CAGAGTTAGC CAGGCCTGCC 
ACCACTCTTT ACCAGCACAA TCTAACCGGA ATACTTGAGA CGGCCGTCAG AGCAACCAAT 
GCCCAATTTG ACAGCCCTGA GATACTGAAG AGGCTTGATG TCCGGCTGCT GGAGGTTTCT 
CCTGGAGACA CTGGCTGGGA CGTCTTCAGT TTGGACTATC ATGTTGAAGG ACCCATTGCC 
ACAGTGTTCA CCCGTGAGTG TATGAGCCAC TACTTGCGTG TCTTCAACTT CCTGTGGAGG 
GCTAAAAGAA TGGAGTACAT CCTCACAGAC ATCTGGAAGG GTCAGATGTG TAACGCCAAG 
CTGCTGAAGA GCATGCCGGA GCTTTCTGGT GTGCTGCACC AGTGTCACGT TCTTGCTTCT 
GAAATGGTTC ACTTCATTCA TCAGATGCAG TATTACATCA CATTTGAGGT GTTGGAATGC 
TCTTGGGATG AGTTGTGGAA TAAAGTCCAG CAGGCTCAGG ATCTGGATCA CATTATAGCA 
GCTCATGACG TTTTCCTGGA CACCATCATT TCTCGCTGCC TGCTGGATGT GAACAACCGG 
TCTCTGTTGA ATCAGTTGCG CGCAGTGTTT GATCAGATCA TCGAGTTCCA GAATGCTCAG 
GACACACTGT ACCGCTCTGC TCTAGAAGAA CTGCAGCTAC GCATACAATT CGAGGACAAG 
AAGAGACAGA GAGAAGAGAT GGCCGAGTGG GGTGTAACAG CAGAGCAGGA GGCCGAGGAG 
AACAGACGCG TGCAGGAGTT TAAAGACACC ATACCAAAAA TGTGCTCTCA GCTCCGATTG 
CTCACACACT TCTATCAGGG CATAGTGCAG GAGTTCCTGC GGCTGCTAAT GACCTGCTCT 
GACGAGAGTC TCCAGTTCCT CAGTTTTCGT CTGGATTTCA ATGAACATTA CAAGGCCCGT 
GACCCCCGCC TGCGGCCCTC GCTCGGGGCC ACCAGAGGCA GACGGAGCTC CAACATCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tubgcp3 ( XM_005170995.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171052.1 Danio rerio tubulin, gamma complex associated protein 3 (tubgcp3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa06123
Clone ID Related Accession (Same CDS sequence)	XM_005170995.3 , XM_005170995.4
Accession Version	XM_005170995.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	gamma-tubulin complex component 3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005170995.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACGC TTGATCAGAA ATCCCCTAAC GTGCTTCTGC AGAGCCTGTG CTGTAAGATC 
ACGGGCAAGA GCGAAGCTGA CGTAGCCCAT CAGTTCCAGT ACGCTGTGCG GGTCATTGGA 
AGTAATTACG CTCCCACAAT TGAACGGGAC GAGTTTTTGG TGTCTGAGAA AATTAAGAAA 
GAATTGTTGA AACAAAGGCG GGAAGCAGAT GCAGCCCTTT TCTCTGAGTT GCACAGAAAG 
CTTCAGACCC AGGGTGTATT GAAACACAGA TGGTCCATCT TATACCTCCT GCTCAGCCTC 
TGTGAGGACC CAAGGAAACC ATCCGGCCGG GTTCCTGTGT GTAGTTATGG AGCTCTGCTT 
GCTCAGGCTT TGCCCCGTGA TGTTCACTCC ACACCGTTCT ACTATGCCCG TCCTCAGAGT 
CTCCCACTCA GCTACCCAGA GCGCTCCGCC ACAGCTCATA ACTCCAGCAT GGCTACCAGT 
GGAATCAGCA GTATGGGGGT GTTTTCTATT AATGGGCCTG GACCGACCCC ACCATCTTTG 
CTTACTGGAG CTGGAACTCA GGCTGTTGGA GAGTCTATGA GAGGGTCTCG TTTAGCCTGG 
ACTCTGCCAG TGTCCAGTTC TCCGTCTGGG GCAGCAGCTG CTCCTCGTCC CCCCAGCGGA 
CCCTCCTCCA GTCCAGCTCC CAAACCACTG CAAAGACCCA GGCGGGACGG AGATGGCAGT 
GCTGATATTG GGGAAGCTGC TCTCGTCCGA GACATTCTTT ATGTTTTCCA GGGAATCGAC 
GGAAAGTTCA TCAAGATGTG CAGCCCGGAA AACTGCTACA AAATTGATTC AAAGGTGCCA 
CTGTGTAAGT CTTTGAGAGA CACTAGCAGC AGGCTGGCCG AGCTCGGCTG GCTCCACAAT 
AAAATTAGAA AATATACAGA CTCACACAGC TTGGATCGAG CCTTTGGTCT TGTTGGGCAG 
AGTTTTTGTG CTGCTCTGCA TCAGGAACTG AAAGAGTACT ACAGATTGCT CTCTGTACTT 
CATGCACAGC TCCAGGTGGA GGACGATCAG GGTGTAAATA TGAGTGTTGA CAGCAGTTTG 
ACTCTGAGGA GACTTCTGGT GTGGACCTAC GACCCCAAGG TTCGACTAAA AACACTGGCC 
GCCCTGGTGG ACTACTGCCA AGGGCGTAAG GGTGGAGAGT TGGCCACTGC TGTACATGCG 
TATGGAAAGA CGGGTGACCC CAACATGAGA GCACTGATCC AGCACATCCT TGGTCTGGTG 
TCTCATCCGA TTCTCAACTT CTTATACCGC TGGATTTATG ATGGAGAGCT GGAGGATACG 
TACCATGAGT TTTTTGTAGC CTCGGACCCA ACAGTGAAGA CGGACAGACT CTGGCATGAC 
AAATACTCTC TAAGGAAGAG TATGATCCCA TCCTTCATCA CCATGGACCA GGCCAGGAAG 
GTTCTTCTTA TTGGCAAATC CATCAACTTC CTGCATCAGG TTTGTCATGA CCGGACACCA 
CCGGGAAAGA TCATGCCTGC CTCCAAATCT AAAGACTCTC CCAAAGATGC TGCAGAGTTG 
TTCACTGATC TGGAAGGGAC CTTTCAGAGT AAGATTGATG CTGCGTATTT TGAGACCAGT 
AAGTATCTTC TAGACGTGCT GAATAAGAAC TACCAGCTTC TGGAGCATCT TCAGGCCATG 
AGGAGATACC TGCTGCTGGG CCAGGGAGAC TTTATCCGGC ACCTCATGGA CCTCCTTAAG 
CCAGAGTTAG CCAGGCCTGC CACCACTCTT TACCAGCACA ATCTAACCGG AATACTTGAG 
ACGGCCGTCA GAGCAACCAA TGCCCAATTT GACAGCCCTG AGATACTGAA GAGGCTTGAT 
GTCCGGCTGC TGGAGGTTTC TCCTGGAGAC ACTGGCTGGG ACGTCTTCAG TTTGGACTAT 
CATGTTGAAG GACCCATTGC CACAGTGTTC ACCCGTGAGT GTATGAGCCA CTACTTGCGT 
GTCTTCAACT TCCTGTGGAG GGCTAAAAGA ATGGAGTACA TCCTCACAGA CATCTGGAAG 
GGTCAGATGT GTAACGCCAA GCTGCTGAAG AGCATGCCGG AGCTTTCTGG TGTGCTGCAC 
CAGTGTCACG TTCTTGCTTC TGAAATGGTT CACTTCATTC ATCAGATGCA GTATTACATC 
ACATTTGAGG TGTTGGAATG CTCTTGGGAT GAGTTGTGGA ATAAAGTCCA GCAGGCTCAG 
GATCTGGATC ACATTATAGC AGCTCATGAC GTTTTCCTGG ACACCATCAT TTCTCGCTGC 
CTGCTGGATG TGAACAACCG GTCTCTGTTG AATCAGTTGC GCGCAGTGTT TGATCAGATC 
ATCGAGTTCC AGAATGCTCA GGACACACTG TACCGCTCTG CTCTAGAAGA ACTGCAGCTA 
CGCATACAAT TCGAGGACAA GAAGAGACAG AGAGAAGAGA TGGCCGAGTG GGGTGTAACA 
GCAGAGCAGG AGGCCGAGGA GAACAGACGC GTGCAGGAGT TTAAAGACAC CATACCAAAA 
ATGTGCTCTC AGCTCCGATT GCTCACACAC TTCTATCAGG GCATAGTGCA GGAGTTCCTG 
CGGCTGCTAA TGACCTGCTC TGACGAGAGT CTCCAGTTCC TCAGTTTTCG TCTGGATTTC 
AATGAACATT ACAAGGCCCG TGACCCCCGC CTGCGGCCCT CGCTCGGGGC CACCAGAGGC 
AGACGGAGCT CCAACATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171052.1
CDS	188..2908
Translation	MATLDQKSPNVLLQSLCCKITGKSEADVAHQFQYAVRVIGSNYAPTIERDEFLVSEKIKKELLKQRREADAALFSELHRKLQTQGVLKHRWSILYLLLSLCEDPRKPSGRVPVCSYGALLAQALPRDVHSTPFYYARPQSLPLSYPERSATAHNSSMATSGISSMGVFSINGPGPTPPSLLTGAGTQAVGESMRGSRLAWTLPVSSSPSGAAAAPRPPSGPSSSPAPKPLQRPRRDGDGSADIGEAALVRDILYVFQGIDGKFIKMCSPENCYKIDSKVPLCKSLRDTSSRLAELGWLHNKIRKYTDSHSLDRAFGLVGQSFCAALHQELKEYYRLLSVLHAQLQVEDDQGVNMSVDSSLTLRRLLVWTYDPKVRLKTLAALVDYCQGRKGGELATAVHAYGKTGDPNMRALIQHILGLVSHPILNFLYRWIYDGELEDTYHEFFVASDPTVKTDRLWHDKYSLRKSMIPSFITMDQARKVLLIGKSINFLHQVCHDRTPPGKIMPASKSKDSPKDAAELFTDLEGTFQSKIDAAYFETSKYLLDVLNKNYQLLEHLQAMRRYLLLGQGDFIRHLMDLLKPELARPATTLYQHNLTGILETAVRATNAQFDSPEILKRLDVRLLEVSPGDTGWDVFSLDYHVEGPIATVFTRECMSHYLRVFNFLWRAKRMEYILTDIWKGQMCNAKLLKSMPELSGVLHQCHVLASEMVHFIHQMQYYITFEVLECSWDELWNKVQQAQDLDHIIAAHDVFLDTIISRCLLDVNNRSLLNQLRAVFDQIIEFQNAQDTLYRSALEELQLRIQFEDKKRQREEMAEWGVTAEQEAEENRRVQEFKDTIPKMCSQLRLLTHFYQGIVQEFLRLLMTCSDESLQFLSFRLDFNEHYKARDPRLRPSLGATRGRRSSNI

Target ORF information:

RefSeq Version	XM_005170995.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio tubulin, gamma complex associated protein 3 (tubgcp3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170995.3

ORF Insert Sequence:

ATGGCGACGC TTGATCAGAA ATCCCCTAAC GTGCTTCTGC AGAGCCTGTG CTGTAAGATC 
ACGGGCAAGA GCGAAGCTGA CGTAGCCCAT CAGTTCCAGT ACGCTGTGCG GGTCATTGGA 
AGTAATTACG CTCCCACAAT TGAACGGGAC GAGTTTTTGG TGTCTGAGAA AATTAAGAAA 
GAATTGTTGA AACAAAGGCG GGAAGCAGAT GCAGCCCTTT TCTCTGAGTT GCACAGAAAG 
CTTCAGACCC AGGGTGTATT GAAACACAGA TGGTCCATCT TATACCTCCT GCTCAGCCTC 
TGTGAGGACC CAAGGAAACC ATCCGGCCGG GTTCCTGTGT GTAGTTATGG AGCTCTGCTT 
GCTCAGGCTT TGCCCCGTGA TGTTCACTCC ACACCGTTCT ACTATGCCCG TCCTCAGAGT 
CTCCCACTCA GCTACCCAGA GCGCTCCGCC ACAGCTCATA ACTCCAGCAT GGCTACCAGT 
GGAATCAGCA GTATGGGGGT GTTTTCTATT AATGGGCCTG GACCGACCCC ACCATCTTTG 
CTTACTGGAG CTGGAACTCA GGCTGTTGGA GAGTCTATGA GAGGGTCTCG TTTAGCCTGG 
ACTCTGCCAG TGTCCAGTTC TCCGTCTGGG GCAGCAGCTG CTCCTCGTCC CCCCAGCGGA 
CCCTCCTCCA GTCCAGCTCC CAAACCACTG CAAAGACCCA GGCGGGACGG AGATGGCAGT 
GCTGATATTG GGGAAGCTGC TCTCGTCCGA GACATTCTTT ATGTTTTCCA GGGAATCGAC 
GGAAAGTTCA TCAAGATGTG CAGCCCGGAA AACTGCTACA AAATTGATTC AAAGGTGCCA 
CTGTGTAAGT CTTTGAGAGA CACTAGCAGC AGGCTGGCCG AGCTCGGCTG GCTCCACAAT 
AAAATTAGAA AATATACAGA CTCACACAGC TTGGATCGAG CCTTTGGTCT TGTTGGGCAG 
AGTTTTTGTG CTGCTCTGCA TCAGGAACTG AAAGAGTACT ACAGATTGCT CTCTGTACTT 
CATGCACAGC TCCAGGTGGA GGACGATCAG GGTGTAAATA TGAGTGTTGA CAGCAGTTTG 
ACTCTGAGGA GACTTCTGGT GTGGACCTAC GACCCCAAGG TTCGACTAAA AACACTGGCC 
GCCCTGGTGG ACTACTGCCA AGGGCGTAAG GGTGGAGAGT TGGCCACTGC TGTACATGCG 
TATGGAAAGA CGGGTGACCC CAACATGAGA GCACTGATCC AGCACATCCT TGGTCTGGTG 
TCTCATCCGA TTCTCAACTT CTTATACCGC TGGATTTATG ATGGAGAGCT GGAGGATACG 
TACCATGAGT TTTTTGTAGC CTCGGACCCA ACAGTGAAGA CGGACAGACT CTGGCATGAC 
AAATACTCTC TAAGGAAGAG TATGATCCCA TCCTTCATCA CCATGGACCA GGCCAGGAAG 
GTTCTTCTTA TTGGCAAATC CATCAACTTC CTGCATCAGG TTTGTCATGA CCGGACACCA 
CCGGGAAAGA TCATGCCTGC CTCCAAATCT AAAGACTCTC CCAAAGATGC TGCAGAGTTG 
TTCACTGATC TGGAAGGGAC CTTTCAGAGT AAGATTGATG CTGCGTATTT TGAGACCAGT 
AAGTATCTTC TAGACGTGCT GAATAAGAAC TACCAGCTTC TGGAGCATCT TCAGGCCATG 
AGGAGATACC TGCTGCTGGG CCAGGGAGAC TTTATCCGGC ACCTCATGGA CCTCCTTAAG 
CCAGAGTTAG CCAGGCCTGC CACCACTCTT TACCAGCACA ATCTAACCGG AATACTTGAG 
ACGGCCGTCA GAGCAACCAA TGCCCAATTT GACAGCCCTG AGATACTGAA GAGGCTTGAT 
GTCCGGCTGC TGGAGGTTTC TCCTGGAGAC ACTGGCTGGG ACGTCTTCAG TTTGGACTAT 
CATGTTGAAG GACCCATTGC CACAGTGTTC ACCCGTGAGT GTATGAGCCA CTACTTGCGT 
GTCTTCAACT TCCTGTGGAG GGCTAAAAGA ATGGAGTACA TCCTCACAGA CATCTGGAAG 
GGTCAGATGT GTAACGCCAA GCTGCTGAAG AGCATGCCGG AGCTTTCTGG TGTGCTGCAC 
CAGTGTCACG TTCTTGCTTC TGAAATGGTT CACTTCATTC ATCAGATGCA GTATTACATC 
ACATTTGAGG TGTTGGAATG CTCTTGGGAT GAGTTGTGGA ATAAAGTCCA GCAGGCTCAG 
GATCTGGATC ACATTATAGC AGCTCATGAC GTTTTCCTGG ACACCATCAT TTCTCGCTGC 
CTGCTGGATG TGAACAACCG GTCTCTGTTG AATCAGTTGC GCGCAGTGTT TGATCAGATC 
ATCGAGTTCC AGAATGCTCA GGACACACTG TACCGCTCTG CTCTAGAAGA ACTGCAGCTA 
CGCATACAAT TCGAGGACAA GAAGAGACAG AGAGAAGAGA TGGCCGAGTG GGGTGTAACA 
GCAGAGCAGG AGGCCGAGGA GAACAGACGC GTGCAGGAGT TTAAAGACAC CATACCAAAA 
ATGTGCTCTC AGCTCCGATT GCTCACACAC TTCTATCAGG GCATAGTGCA GGAGTTCCTG 
CGGCTGCTAA TGACCTGCTC TGACGAGAGT CTCCAGTTCC TCAGTTTTCG TCTGGATTTC 
AATGAACATT ACAAGGCCCG TGACCCCCGC CTGCGGCCCT CGCTCGGGGC CACCAGAGGC 
AGACGGAGCT CCAACATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

tubgcp3 ( XM_005170995.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005171052.1 Danio rerio tubulin, gamma complex associated protein 3 (tubgcp3), transcript variant X1, mRNA.

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CloneID	ODa06123
Clone ID Related Accession (Same CDS sequence)	XM_005170995.3 , XM_005170995.4
Accession Version	XM_005170995.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2721bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	gamma-tubulin complex component 3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007112.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005170995.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGACGC TTGATCAGAA ATCCCCTAAC GTGCTTCTGC AGAGCCTGTG CTGTAAGATC 
ACGGGCAAGA GCGAAGCTGA CGTAGCCCAT CAGTTCCAGT ACGCTGTGCG GGTCATTGGA 
AGTAATTACG CTCCCACAAT TGAACGGGAC GAGTTTTTGG TGTCTGAGAA AATTAAGAAA 
GAATTGTTGA AACAAAGGCG GGAAGCAGAT GCAGCCCTTT TCTCTGAGTT GCACAGAAAG 
CTTCAGACCC AGGGTGTATT GAAACACAGA TGGTCCATCT TATACCTCCT GCTCAGCCTC 
TGTGAGGACC CAAGGAAACC ATCCGGCCGG GTTCCTGTGT GTAGTTATGG AGCTCTGCTT 
GCTCAGGCTT TGCCCCGTGA TGTTCACTCC ACACCGTTCT ACTATGCCCG TCCTCAGAGT 
CTCCCACTCA GCTACCCAGA GCGCTCCGCC ACAGCTCATA ACTCCAGCAT GGCTACCAGT 
GGAATCAGCA GTATGGGGGT GTTTTCTATT AATGGGCCTG GACCGACCCC ACCATCTTTG 
CTTACTGGAG CTGGAACTCA GGCTGTTGGA GAGTCTATGA GAGGGTCTCG TTTAGCCTGG 
ACTCTGCCAG TGTCCAGTTC TCCGTCTGGG GCAGCAGCTG CTCCTCGTCC CCCCAGCGGA 
CCCTCCTCCA GTCCAGCTCC CAAACCACTG CAAAGACCCA GGCGGGACGG AGATGGCAGT 
GCTGATATTG GGGAAGCTGC TCTCGTCCGA GACATTCTTT ATGTTTTCCA GGGAATCGAC 
GGAAAGTTCA TCAAGATGTG CAGCCCGGAA AACTGCTACA AAATTGATTC AAAGGTGCCA 
CTGTGTAAGT CTTTGAGAGA CACTAGCAGC AGGCTGGCCG AGCTCGGCTG GCTCCACAAT 
AAAATTAGAA AATATACAGA CTCACACAGC TTGGATCGAG CCTTTGGTCT TGTTGGGCAG 
AGTTTTTGTG CTGCTCTGCA TCAGGAACTG AAAGAGTACT ACAGATTGCT CTCTGTACTT 
CATGCACAGC TCCAGGTGGA GGACGATCAG GGTGTAAATA TGAGTGTTGA CAGCAGTTTG 
ACTCTGAGGA GACTTCTGGT GTGGACCTAC GACCCCAAGG TTCGACTAAA AACACTGGCC 
GCCCTGGTGG ACTACTGCCA AGGGCGTAAG GGTGGAGAGT TGGCCACTGC TGTACATGCG 
TATGGAAAGA CGGGTGACCC CAACATGAGA GCACTGATCC AGCACATCCT TGGTCTGGTG 
TCTCATCCGA TTCTCAACTT CTTATACCGC TGGATTTATG ATGGAGAGCT GGAGGATACG 
TACCATGAGT TTTTTGTAGC CTCGGACCCA ACAGTGAAGA CGGACAGACT CTGGCATGAC 
AAATACTCTC TAAGGAAGAG TATGATCCCA TCCTTCATCA CCATGGACCA GGCCAGGAAG 
GTTCTTCTTA TTGGCAAATC CATCAACTTC CTGCATCAGG TTTGTCATGA CCGGACACCA 
CCGGGAAAGA TCATGCCTGC CTCCAAATCT AAAGACTCTC CCAAAGATGC TGCAGAGTTG 
TTCACTGATC TGGAAGGGAC CTTTCAGAGT AAGATTGATG CTGCGTATTT TGAGACCAGT 
AAGTATCTTC TAGACGTGCT GAATAAGAAC TACCAGCTTC TGGAGCATCT TCAGGCCATG 
AGGAGATACC TGCTGCTGGG CCAGGGAGAC TTTATCCGGC ACCTCATGGA CCTCCTTAAG 
CCAGAGTTAG CCAGGCCTGC CACCACTCTT TACCAGCACA ATCTAACCGG AATACTTGAG 
ACGGCCGTCA GAGCAACCAA TGCCCAATTT GACAGCCCTG AGATACTGAA GAGGCTTGAT 
GTCCGGCTGC TGGAGGTTTC TCCTGGAGAC ACTGGCTGGG ACGTCTTCAG TTTGGACTAT 
CATGTTGAAG GACCCATTGC CACAGTGTTC ACCCGTGAGT GTATGAGCCA CTACTTGCGT 
GTCTTCAACT TCCTGTGGAG GGCTAAAAGA ATGGAGTACA TCCTCACAGA CATCTGGAAG 
GGTCAGATGT GTAACGCCAA GCTGCTGAAG AGCATGCCGG AGCTTTCTGG TGTGCTGCAC 
CAGTGTCACG TTCTTGCTTC TGAAATGGTT CACTTCATTC ATCAGATGCA GTATTACATC 
ACATTTGAGG TGTTGGAATG CTCTTGGGAT GAGTTGTGGA ATAAAGTCCA GCAGGCTCAG 
GATCTGGATC ACATTATAGC AGCTCATGAC GTTTTCCTGG ACACCATCAT TTCTCGCTGC 
CTGCTGGATG TGAACAACCG GTCTCTGTTG AATCAGTTGC GCGCAGTGTT TGATCAGATC 
ATCGAGTTCC AGAATGCTCA GGACACACTG TACCGCTCTG CTCTAGAAGA ACTGCAGCTA 
CGCATACAAT TCGAGGACAA GAAGAGACAG AGAGAAGAGA TGGCCGAGTG GGGTGTAACA 
GCAGAGCAGG AGGCCGAGGA GAACAGACGC GTGCAGGAGT TTAAAGACAC CATACCAAAA 
ATGTGCTCTC AGCTCCGATT GCTCACACAC TTCTATCAGG GCATAGTGCA GGAGTTCCTG 
CGGCTGCTAA TGACCTGCTC TGACGAGAGT CTCCAGTTCC TCAGTTTTCG TCTGGATTTC 
AATGAACATT ACAAGGCCCG TGACCCCCGC CTGCGGCCCT CGCTCGGGGC CACCAGAGGC 
AGACGGAGCT CCAACATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005171052.1
CDS	178..2898
Translation	MATLDQKSPNVLLQSLCCKITGKSEADVAHQFQYAVRVIGSNYAPTIERDEFLVSEKIKKELLKQRREADAALFSELHRKLQTQGVLKHRWSILYLLLSLCEDPRKPSGRVPVCSYGALLAQALPRDVHSTPFYYARPQSLPLSYPERSATAHNSSMATSGISSMGVFSINGPGPTPPSLLTGAGTQAVGESMRGSRLAWTLPVSSSPSGAAAAPRPPSGPSSSPAPKPLQRPRRDGDGSADIGEAALVRDILYVFQGIDGKFIKMCSPENCYKIDSKVPLCKSLRDTSSRLAELGWLHNKIRKYTDSHSLDRAFGLVGQSFCAALHQELKEYYRLLSVLHAQLQVEDDQGVNMSVDSSLTLRRLLVWTYDPKVRLKTLAALVDYCQGRKGGELATAVHAYGKTGDPNMRALIQHILGLVSHPILNFLYRWIYDGELEDTYHEFFVASDPTVKTDRLWHDKYSLRKSMIPSFITMDQARKVLLIGKSINFLHQVCHDRTPPGKIMPASKSKDSPKDAAELFTDLEGTFQSKIDAAYFETSKYLLDVLNKNYQLLEHLQAMRRYLLLGQGDFIRHLMDLLKPELARPATTLYQHNLTGILETAVRATNAQFDSPEILKRLDVRLLEVSPGDTGWDVFSLDYHVEGPIATVFTRECMSHYLRVFNFLWRAKRMEYILTDIWKGQMCNAKLLKSMPELSGVLHQCHVLASEMVHFIHQMQYYITFEVLECSWDELWNKVQQAQDLDHIIAAHDVFLDTIISRCLLDVNNRSLLNQLRAVFDQIIEFQNAQDTLYRSALEELQLRIQFEDKKRQREEMAEWGVTAEQEAEENRRVQEFKDTIPKMCSQLRLLTHFYQGIVQEFLRLLMTCSDESLQFLSFRLDFNEHYKARDPRLRPSLGATRGRRSSNI

Target ORF information:

RefSeq Version	XM_005170995.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio tubulin, gamma complex associated protein 3 (tubgcp3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170995.4

ORF Insert Sequence:

ATGGCGACGC TTGATCAGAA ATCCCCTAAC GTGCTTCTGC AGAGCCTGTG CTGTAAGATC 
ACGGGCAAGA GCGAAGCTGA CGTAGCCCAT CAGTTCCAGT ACGCTGTGCG GGTCATTGGA 
AGTAATTACG CTCCCACAAT TGAACGGGAC GAGTTTTTGG TGTCTGAGAA AATTAAGAAA 
GAATTGTTGA AACAAAGGCG GGAAGCAGAT GCAGCCCTTT TCTCTGAGTT GCACAGAAAG 
CTTCAGACCC AGGGTGTATT GAAACACAGA TGGTCCATCT TATACCTCCT GCTCAGCCTC 
TGTGAGGACC CAAGGAAACC ATCCGGCCGG GTTCCTGTGT GTAGTTATGG AGCTCTGCTT 
GCTCAGGCTT TGCCCCGTGA TGTTCACTCC ACACCGTTCT ACTATGCCCG TCCTCAGAGT 
CTCCCACTCA GCTACCCAGA GCGCTCCGCC ACAGCTCATA ACTCCAGCAT GGCTACCAGT 
GGAATCAGCA GTATGGGGGT GTTTTCTATT AATGGGCCTG GACCGACCCC ACCATCTTTG 
CTTACTGGAG CTGGAACTCA GGCTGTTGGA GAGTCTATGA GAGGGTCTCG TTTAGCCTGG 
ACTCTGCCAG TGTCCAGTTC TCCGTCTGGG GCAGCAGCTG CTCCTCGTCC CCCCAGCGGA 
CCCTCCTCCA GTCCAGCTCC CAAACCACTG CAAAGACCCA GGCGGGACGG AGATGGCAGT 
GCTGATATTG GGGAAGCTGC TCTCGTCCGA GACATTCTTT ATGTTTTCCA GGGAATCGAC 
GGAAAGTTCA TCAAGATGTG CAGCCCGGAA AACTGCTACA AAATTGATTC AAAGGTGCCA 
CTGTGTAAGT CTTTGAGAGA CACTAGCAGC AGGCTGGCCG AGCTCGGCTG GCTCCACAAT 
AAAATTAGAA AATATACAGA CTCACACAGC TTGGATCGAG CCTTTGGTCT TGTTGGGCAG 
AGTTTTTGTG CTGCTCTGCA TCAGGAACTG AAAGAGTACT ACAGATTGCT CTCTGTACTT 
CATGCACAGC TCCAGGTGGA GGACGATCAG GGTGTAAATA TGAGTGTTGA CAGCAGTTTG 
ACTCTGAGGA GACTTCTGGT GTGGACCTAC GACCCCAAGG TTCGACTAAA AACACTGGCC 
GCCCTGGTGG ACTACTGCCA AGGGCGTAAG GGTGGAGAGT TGGCCACTGC TGTACATGCG 
TATGGAAAGA CGGGTGACCC CAACATGAGA GCACTGATCC AGCACATCCT TGGTCTGGTG 
TCTCATCCGA TTCTCAACTT CTTATACCGC TGGATTTATG ATGGAGAGCT GGAGGATACG 
TACCATGAGT TTTTTGTAGC CTCGGACCCA ACAGTGAAGA CGGACAGACT CTGGCATGAC 
AAATACTCTC TAAGGAAGAG TATGATCCCA TCCTTCATCA CCATGGACCA GGCCAGGAAG 
GTTCTTCTTA TTGGCAAATC CATCAACTTC CTGCATCAGG TTTGTCATGA CCGGACACCA 
CCGGGAAAGA TCATGCCTGC CTCCAAATCT AAAGACTCTC CCAAAGATGC TGCAGAGTTG 
TTCACTGATC TGGAAGGGAC CTTTCAGAGT AAGATTGATG CTGCGTATTT TGAGACCAGT 
AAGTATCTTC TAGACGTGCT GAATAAGAAC TACCAGCTTC TGGAGCATCT TCAGGCCATG 
AGGAGATACC TGCTGCTGGG CCAGGGAGAC TTTATCCGGC ACCTCATGGA CCTCCTTAAG 
CCAGAGTTAG CCAGGCCTGC CACCACTCTT TACCAGCACA ATCTAACCGG AATACTTGAG 
ACGGCCGTCA GAGCAACCAA TGCCCAATTT GACAGCCCTG AGATACTGAA GAGGCTTGAT 
GTCCGGCTGC TGGAGGTTTC TCCTGGAGAC ACTGGCTGGG ACGTCTTCAG TTTGGACTAT 
CATGTTGAAG GACCCATTGC CACAGTGTTC ACCCGTGAGT GTATGAGCCA CTACTTGCGT 
GTCTTCAACT TCCTGTGGAG GGCTAAAAGA ATGGAGTACA TCCTCACAGA CATCTGGAAG 
GGTCAGATGT GTAACGCCAA GCTGCTGAAG AGCATGCCGG AGCTTTCTGG TGTGCTGCAC 
CAGTGTCACG TTCTTGCTTC TGAAATGGTT CACTTCATTC ATCAGATGCA GTATTACATC 
ACATTTGAGG TGTTGGAATG CTCTTGGGAT GAGTTGTGGA ATAAAGTCCA GCAGGCTCAG 
GATCTGGATC ACATTATAGC AGCTCATGAC GTTTTCCTGG ACACCATCAT TTCTCGCTGC 
CTGCTGGATG TGAACAACCG GTCTCTGTTG AATCAGTTGC GCGCAGTGTT TGATCAGATC 
ATCGAGTTCC AGAATGCTCA GGACACACTG TACCGCTCTG CTCTAGAAGA ACTGCAGCTA 
CGCATACAAT TCGAGGACAA GAAGAGACAG AGAGAAGAGA TGGCCGAGTG GGGTGTAACA 
GCAGAGCAGG AGGCCGAGGA GAACAGACGC GTGCAGGAGT TTAAAGACAC CATACCAAAA 
ATGTGCTCTC AGCTCCGATT GCTCACACAC TTCTATCAGG GCATAGTGCA GGAGTTCCTG 
CGGCTGCTAA TGACCTGCTC TGACGAGAGT CTCCAGTTCC TCAGTTTTCG TCTGGATTTC 
AATGAACATT ACAAGGCCCG TGACCCCCGC CTGCGGCCCT CGCTCGGGGC CACCAGAGGC 
AGACGGAGCT CCAACATCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Tissue-restricted expression of Nrf2 and its target genes in zebrafish with gene-specific variations in the induction profiles.
PloS one6(10)e26884(2011)
Nakajima H,Nakajima-Takagi Y,Tsujita T,Akiyama S,Wakasa T,Mukaigasa K,Kaneko H,Tamaru Y,Yamamoto M,Kobayashi M

Transcriptomic analyses reveal novel genes with sexually dimorphic expression in the zebrafish gonad and brain.
PloS one3(3)e1791(2008 Mar)
Sreenivasan R,Cai M,Bartfai R,Wang X,Christoffels A,Orban L

Soluble tubulin complexes, gamma-tubulin, and their changing distribution in the zebrafish (Danio rerio) ovary, oocyte and embryo.
Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology147(1)56-73(2007 May)
Liu J,Lessman CA