flna gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_021474524.1	XP_021330199.1	filamin-A isoform X2
XM_009296771.2	XP_009295046.1	filamin-A isoform X2
XM_009296770.2	XP_009295045.1	filamin-A isoform X1
XM_021473622.1	XP_021329297.1	filamin-A isoform X1
XM_021473624.1	XP_021329299.1	filamin-A isoform X3
XM_021474525.1	XP_021330200.1	filamin-A isoform X3
XM_021474523.1	XP_021330198.1	filamin-A isoform X1
XM_009296771.3	XP_009295046.1	filamin-A isoform X2
XM_017353690.2	XP_017209179.1	filamin-A isoform X3
XM_021473623.1	XP_021329298.1	filamin-A isoform X2
XM_017353690.1	XP_017209179.1	filamin-A isoform X3
XM_009296770.3	XP_009295045.1	filamin-A isoform X1

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa146250	XM_021473622.1 Latest version!	Danio rerio filamin A, alpha (actin binding protein 280) (flna), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa146251	XM_021473623.1 Latest version!	Danio rerio filamin A, alpha (actin binding protein 280) (flna), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa146252	XM_021473624.1 Latest version!	Danio rerio filamin A, alpha (actin binding protein 280) (flna), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa57477	XM_017353690.2 Latest version!	Danio rerio filamin A, alpha (actin binding protein 280) (flna), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa57477	XM_021474525.1 Latest version!	Danio rerio filamin A, alpha (actin binding protein 280) (flna), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa43841	XM_021474523.1 Latest version!	Danio rerio filamin A, alpha (actin binding protein 280) (flna), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa43841	XM_009296770.3 Latest version!	Danio rerio filamin A, alpha (actin binding protein 280) (flna), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa43842	XM_009296771.3 Latest version!	Danio rerio filamin A, alpha (actin binding protein 280) (flna), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa43842	XM_021474524.1 Latest version!	Danio rerio filamin A, alpha (actin binding protein 280) (flna), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
View Old Accession Versions >>
ODa43842	XM_009296771.2	Danio rerio filamin A, alpha (actin binding protein 280) (flna), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
ODa57477	XM_017353690.1	Danio rerio filamin A, alpha (actin binding protein 280) (flna), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1371.30 $1959.00
ODa43841	XM_009296770.2	Danio rerio filamin A, alpha (actin binding protein 280) (flna), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	30	$1371.30 $1959.00
Hide Old Accession Versions >>

flna ( XM_021473622.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329297.1 Danio rerio filamin A, alpha (actin binding protein 280) (flna), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa146250
Clone ID Related Accession (Same CDS sequence)	XM_021473622.1
Accession Version	XM_021473622.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	filamin-A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395142.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCAAC ACACACGCCC CCACCAGTCG AGCGCCGCCG CTCCCCTATC CAACGCTTCT 
CTCTCCCCGG ACAAGGACGC CGACATGCCC GCTACAGAGA AAGATCTCGC CGAGGACGCG 
CCATGGAAGA AAATCCAGCA AAATACATTC ACGCGTTGGT GCAACGAGCA TCTGAAATGT 
GTCAACAAGC GCATAGCGAA CCTGCAGACG GACCTGAGCG ACGGGCTCCG GCTGATCGCG 
CTTCTGGAGG TGCTCAGCCA GAAGAAGATG TTCCGCAAGT ACAACCAGCG CCCTACCTTC 
AGACAGATGC AGCTGGAGAA CGTCTCCGTG GCGCTGGAGT TCCTGGACAT TGAAAACATC 
AAGCTGGTCT CCATTGACAG TAAGGCCATA GTGGATGGTA ACCTGAAGCT GATCCTGGGT 
CTGATATGGA CTCTTATTCT GCATTATTCC ATCTCCATGC CCATGTGGGA CGAGGAAGAG 
GAGAGCGAGG AAAGCAAGCA GAAGACGCCC AAGCAGAGGC TCCTCGGATG GATCCAGAAC 
AAGCTGCCCG AGCTGCCAAT CACCAACTTC AGCCGCGATT GGCAGTCAGG GAAAGCCCTT 
GGCGCTCTGG TGGACAGTTG TGCTCCAGGT CTGTGTCCAG ACTGGGATTC CTGGGACCAG 
ACCAAGCCTG TGGACAATGC CAGAGAGGCC ATGCAGCAGG CTGATGACTG GCTCGGTGTT 
CCTCAGGTCA TCACACCAGA AGAAATCGTT GATCCCAATG TGGACGAGCA CTCCGTTATG 
ACTTACCTGT CCCAGTTTCC CAAAGCCAAA CTCAAGCCTG GTGCCCCTCT GAGGCCCAAA 
CTCAATCCCA AAAAGGCCCG TGCTTATGGA CCAGGTATTG AGCCAACAGG TAATGTTGTG 
ATGAAGAAGG CTGTGTTCAC TGTTGAGACC ATCAGCGCTG GTCAGGGTGA GGTACTGGTT 
TATGTCGAGG ACCCCGCTGG ACACCGCGAG GAGGCCAAAG TCACAGCCAA CAATGACAAG 
AACCGCACCT ACTCAGTATT CTATGTGCCA AAAGTCACTG GACAACACAA GGTGACAGTG 
CTGTTTGCAG GGCAACACAT CTCTAAGAGC CCGTTTGAGG TGGATGTGGG AATGGCTCAG 
GGAGACTCCA GCAAGGTCAC TGCCCAGGGC CCAGGACTCG AGCCTGCAGG CAACATTGCC 
AACAAAACCA CATACTTTGA TGTCTACACA GCTGGTGCTG GAGTAGGAGA GGTGGAGGTG 
GTCATCATGG ACCCCAGTGG AAAGAAGGAC ACGGTTGAGT GCAGTGTCGA GGATAAAGGC 
AACAGCAGCT ACCGATGCAC CTACAAGCCC ACTCAGGAGG GACAGCACAT CATTTACATC 
ACCTTTGCCG GGGGACAGAT CTCCAAGAGC CCTTACACTG TCAATGTGGG AGAGGCCTGT 
AATCCAAGCC TGTGTACAGC CAAGGGCCGC GGTCTGCAGC CTAAAGGCCT GAGGGTCAAG 
GAGACCGCCG AGTTTAAGGT TTACACCAAA GGAGCAGGAA CCGGAGACCT CAAAGTTACC 
ATCAAAGGGC CCAAGGGTCT CGAGGAGCCC TGTAAGAAGA AGGATTTGGG AGACGGCGTG 
TACAGCTTTG ATTATTACCC CTCCACACCT GGAAATTACA TCATCACCAT CACATGGGGC 
GGTCAACACA TCCCACGCAG CCCGTTTGAG GTGAAGATCG GGTCTGAAGC AGGACCGCAG 
CAGGTCAGGG CGTGGGGTCC AGGACTAGAG AGTGGCGTTG TGGGCAAATC TGCTGACTTT 
GTTGTGGAAG CTGTTGGTGA TGATGTGGGC ACTTTAGGTT TCTCTGTTGA AGGCCCTTCC 
CCGGCTAAGA TTGAATGTGA TGATAAGGGA GATGGTTCAT GTGACGTGAG GTACTGGCCC 
ACCGAACCGG GTGAATATGC AGTTCATGTG CTCTGTAAGA ATGAGGACAT CCAGCTCAGC 
CCTTTCATGG CTGAAATCAT GCCCGCCCCA GGAAAAGACT TTTATCCTGA CAAGGTGAAG 
GCTTATGGCC CCGGTCTGCA GAGCTCAGGC CTGTCAGTTG GCAAACCAGC CGAGTTTACC 
GTGGATGCCA AACTGGGTGG CAAAGCTCCT CTGAAGATTC AAGCTCAGGA CCGTGATGGA 
AACCCAGTCG ATGTGCAGGT GAAGGACAAT GGAAATGGGA CATACAGCTG CAGCTACACC 
CCTCGCAAAC CAGTCAAACA CACTGTGATG GTGTCCTGGG GCGGCGTCAA CATCCCAGAG 
AGCCCGTTCA GAATGAACAT TGGGGCAGGG TGTCATCCCA ATAAGGTGAA AGTATCTGGA 
CCTGGAGTGG CCAAGACAGG CCTCAAAGCA TATGATCCAA CATACTTTAC TGTGGATTGT 
GCTGAGGCTG GACAGGGCGA CATCAGCATA GGCATCAAAT GTGCTGCAGG TGTGGTGGGA 
CCTGCTGAGG CTGACATAGA CTTCGACATC ATTAGAAATG ACAACGACAC ATTTACTGTT 
AAATACACTC CTCCTGGAGC GGGCAGCTAC ACCATCATGG TGCTGTTTGC TGATCAGACC 
ATTCCCATGA CACCCATCAG AATTAAGGTT GATCCCTCTC ATGATGCAAG CAAGGTTAAA 
GCTGAGGGGC CTGGACTTAG CCGTTCTGGT GTGGAGCTGA ACAAACCCAC TCACTTCACT 
GTGAACACAA AGGCTGCAGG CAAGGCCAAG CTTGATGCTC AGTTCACTGG ACCCAACAAG 
GGAGATGCAG TCCGTGACTT CGACATAATC GACAACCATG ATGGCACTCA CACTGTCAAA 
TATACCCCTG TCCAACAGGG CAACATGGGT TTGAATGTCA CCTACGGTGG TGACCCTATT 
CCCAAGAGCC CATTTGCAGT AGCAATTGCC CCCTCTCTGG ACCTCAGTAA AGTTAAAGTG 
GCTGGTCTTG GAAGTAAAAT GACTGTTGGA AAGGATCAGG AGATCACAGT CAAATCTAAA 
GGTGCAGGCG GTCAGGGCAA AGTTGGCGCA AAGGTTACTG GTCCATCTGC TAAATCAGTG 
CCCTGTAAAG TTGAACCAGG CCTGAGCCCA GAAACCAGCC AAGTTCGTTT TATCCCAAGA 
GATAAGGGAC CCTATGAGGT GGAACTGACA TATGATGGCG CCCCCATCCC TGGCAGTCCA 
TTCCCCATCG AAGCCATTGC ACCTACTGAC CCATCCAAGG TGCGATGCTC TGGGCCAGGA 
CTGGAGCGGG CGAAGGTTGG CGAGCCTGGA CAGTTTGTGG TGGATTGCAC TAATGCTGGC 
CCTGCTGAGT TGACCATCGA GATCATCTCT GACAATGGCA CTGAGGCTGA AGTCCACATT 
CAAGACAACG GCGACGGGAC CTACACCATC ACTTACGTCC CTCTCTACCC TGGAGCCTAT 
ACCCTCACCA TCCGCTACGG GGACCAGGAT GTGCCAAACT TCCCAGCAAG ACTCCATGTG 
GAGCCTGCTG TGGAGACCAG TGGAGTGAAA GTGTTCGGAC CCGGAGTGGA GGGCAAAGGT 
GTTTTCAGGG AAGCCACTAC AGATTTCACT GTGGATGCCC GTGCGCTCAC TAAAACCGGA 
GGCAATCACA TCAAAACCTG CATCAACAAC CCATCGGGCA ACCGCACTGA AGCACTGATC 
CGAGATCTGG GAGACGGCAC ATATCAAGTG GAGTACACGC CTTATGAGGA GGGTACACAC 
AGTGTTGAGG TTGCATATGA CAGCTCCCCG GTTCCCAAAA GCCCCTTCCG TGTGCCAGTG 
ACCGAGGGCT GTGACCCGGC GCGTGTGCGT GTGCATGGTC CAGGACTTCA GTCTGGCATC 
ACCAACAAAC CTAACAAATT TACCGTGGAG ACCCGTGGGG CTGGTACTGG TGGACTGGGG 
CTGGCTATGG AAGGACCTTC TGAGGCCAAA ATGTCCTGCA CCGATAACAA AGATGGCAGC 
TGTTGTGTTG AATACATCCC ATATGAGCCA GGGACATACA ACCTCAATGT CACCTATGGA 
GGCCAACCAA TCACTGGAAG CCCTTTCTCT GTACCTGTCC ATGATACTGT TGACCCAACT 
AAAGTGAAAT GTCAGGGCCA GGGGCTTGGA AACAACGTGC GTGCCAATAT CCCACAGGTC 
TTTTCTGTGG ATGCAAGCAA GGCTGGTATG GCGCCACTGC AGGTCAGAGT TCAGGGACCC 
AAAGGTGTTG TGGAGCCTGC TGAGGTGGTG GATAATGGTG ACCAGACTCA CACAGTCAGC 
TATGTGCCCA CAAGAGAGGG GCCATACTCC ATTAATGTTC TGTATGCTGA TGAGGAGATC 
CCACAGAGTC CTTATAAGGT GAAGGTTCTG CCCACTCATG ACGCAAGTAA AGTCCGTTGC 
AGCGGTCCTG GCCTGAATAC CACTGGTGTT CCTGCCAGTC TGCCCGTGGA GTTCACTATT 
GACGCCAAAG ATGCAGGAGA GGGACTGTTG GCCGTCCAGA TAACTGACCC TGATGGGAAG 
CCAAAGAAGG CTAACATTCG TGATAATCAG GACGGGACCT ACCTTGTGTC CTATGTGCCT 
GACATGACCG GCCGTTACAC CATTCTTATT AAGTATGGTG GTGATGAGAT CCCGTATTCT 
CCATATCGCA TTCGAGCCCT GCCTACTGGA GACGCCAGCA AGTGTACAGT CACAGTCTCA 
ATTGGAGGTC ATGGTTTGGG AGCTGGAGTT GGTCCGACTA TTCAGATTGG AGAGCAGACT 
GTCATCACTG TGGATGCGAA GGCAGCTGGG AAGGGCAAGG TGACCTGTAC CGTGTGCACA 
CCTGATGGAG GCGAGGTGGA TGTGGACGTC GTGGAAAATG AGGATGGAAC GTTTGACATC 
TTTTACACTG CACCGCAGCC TGGGAAATAC GTGATCTGTG TGCGCTTCGG TGGAGAGCAC 
ATCCCTAACA GCCCCTTCCA AGTCACGGCT TTGGAAGGTG ACTCTCCTGA CCAACTAATG 
CAGCAGAGCC AGAACCTGCA GTACGCCTAC GCGCCCAACA TGGGCCAGAC ATGGGCTACA 
GATGGGCAAT TGGGTATGAA TGGACTGGAT GTAGCTGGAC TGAGACCCTT TGACTTGGTC 
ATTCCTTTCA CCATTCAGAA AGGAGAAATT ACAGGTGATG TACGAATGCC CTCTGGAAAA 
GTGGCCAAGC CTGATATTAC AGACAACAAG GATGGCACAG TCACAGTGAA ATATGCCCCA 
ACTGAGGCGG GCCTGCATGA GATGGACATT AAATATGATG GAATACACAT TCCAGGAAGT 
CCATTGCAAT TCTACGTGGA TTATGTCAAC AGCGGTCATG TGACTGCATA TGGTCCTGGT 
CTAATTCATG GCATGGTCAA TAAGCCTGCA GTCTTCACAG TCAACACAAA GGATGCTGGA 
GAGGGTGGTC TGTCTCTGGC CATCGAGGGG CCATCAAAGG CAGATATCAG CTGCACTGAT 
AATCAGGACG GTACCTGCAC TGTTTCATAC CTCCCTGTTC TTCCCGGAGA CTACAATATC 
ATTGTTAAAT ACAATGACAA GAACATCCCC GGCAGCCCTT TCATGTCCAA AATCACAGGT 
GACGACTCGA TGCGCATGTC TCACCTGAAG GTGGGCTCAG CTGCTGACAT CCCTCTGGAC 
ATTGGTGAGC TTGACCTCAG TCAGCTGACA GCCACACTCA CCACCCCATC CGGCCGAGAG 
GAGCCTTGCC TGCTCAAGAT GCTCAGAAAT GGGCATGTGG GAATTTCCTT TGTGCCCAAG 
GAAATTGGCG AGCATCTTGT TAACATTAAG AAAAATGGAC GCCATATTCC TAGTAGCCCC 
ATTTCTGTCA TGATCAACCA ATCAGAGATC GGTGATGCCA GCCGTGTACG AGTAACTGGT 
CAAGGCCTGA GTGAAGCTCG CACCTTTGAG CCCGCAGAAT TCATCATTGA CACTAGAGAT 
GCAGGATATG GTGGACTCAG TTTGTCTATT GAGGGACCTA GTAAAGTGGA TATCAACACT 
GAAGATCAGG AAGATGGCAC CTGCAAAGTG ACATATTGCC CCACAGAGCC TGGAAACTAC 
ATAATTAACA TCAAATTTGC AGACCAGCAT GTGCCAGGAA GTGCTTTTAC TGTCAAAGTG 
ACAGGCGAGG GTCGGATGAA GGAGAGCATC ACCCGCAAAA GAAGAGCTGC TTCTGTGGCC 
AATGTTGGCA GCCAGTGCGA CCTCAGTCTA AAGATCCCTG AGATCAGCAT TGCAGACATG 
ACCGCTCAGG TGACCAGCCC ATCTGGCAAA GTCCACAAGG CAGAGATAAT GGAGGGAGAA 
AACAACACCT ACTGCATCCG ATTCGTGCCC ACAGAGATGG GTGTGCACAC AGTAAGCGTG 
AAGTATCAAG GTCAGCATGT GCCTGGATCC CCCTTCCAGT TCACGGTGGG ACCTCTTGGA 
GAAGGAGGAG CTCATAAAGT CAGAGCTGGA GGCCCAGGAT TAGAGAGGGC AGAAGCTGGT 
GTACCAGCGG AGTTCAGCAT TTGGACTCGT GAAGCAGGAG CTGGTGGTCT GTCCATCGCA 
GTGGAAGGAC CCAGCAAAGC AGAGATTGCT TTTGAAGACC GTAAAGATGG ATCCAGTGGT 
GTCTCTTACA TCGTCCAGGA GCCTGGCGAT TATGAAGTAT CCATCAAGTT CAATGACGAG 
CACATCCCTG ACAGTCCGTT TGTGGTGCCT GTGGCCTCGC CATCTGACGA TGCCCGCCGT 
CTCACTGTTG CCAGTCTTCA GGAGTCCGGG TTAAAGGTGA ACCAGCCGGC CTCTTTTGCT 
GTGAGCCTAA ATGGGGCGAA AGGAGTCATC GATGCCAAAG TTCACAGTCC ATCAGGAGCA 
CTTGAAGAGT GCTGCGTCAC AGAGATCGAT GAAGATAAAT ATGCTGTGAG ATTCATTCCC 
AGGGAGAATG GCCTGTACCT GATAGATGTG AAATTTAATG GTTCTCACAT CCCTGGAAGC 
CCCTTCAAGA TCCGTGTTGG TGAGACGGGT CAGGCTGGAG ATCCAGGAAT GGTGTCTGCT 
TATGGAGCTG GTCTGGAAGG AGGCACCACT GGATCGCCTT GTGAGTTCAT TGTAAACACC 
AGTTCAGCTG GCCCTGGAGC TTTGGCGGTG ACTATCGACG GGCCGTCCAA GGTGAAGATG 
GACTGTCAGG AATGCCCAGA GGGCTACAAA GTCACCTACA CACCTATGGC CCCTGGAAAC 
TACCTAATTT CTATTAAATA CGGTGGACCG TACCATATTG TTGGTAGTCC ATTCAAAGCT 
AAAATTACTG GCTCCCGATT GGTCAACAGC CACAGCATGC ATGAGACTTC TTCAGTACTG 
GTTGACCCTG TGACCCGCAG CATGACGACC AGCCAGCAGG CAGCGCCAGG CTGGGCCAGC 
TCTGATGCCA GCCGCGTAGT GGCCAAAGGT TTGGGGCTCA ACAAGGGCTT CATAGGACAG 
AAGAACACTT TCAGTGTGGA CTGTAGTAAA GCAGGGAGGA ACATGCTCCT GGTCGGAGTG 
GATGGACCCA AAGTGCCCTG TGAGGAGATC CTGGTGAAAC ACCTGGGCAA CCGCCTCTAC 
AACGTTTCCT ACCAGCTGAA GGAGAAAGGA GAGTACATCC TGGTGGTCAA ATGGGGTGAT 
GAGCACATCC CGGGCAGCCC GTATCACATC ACTGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329297.1
CDS	352..8010
Translation	MSQHTRPHQSSAAAPLSNASLSPDKDADMPATEKDLAEDAPWKKIQQNTFTRWCNEHLKCVNKRIANLQTDLSDGLRLIALLEVLSQKKMFRKYNQRPTFRQMQLENVSVALEFLDIENIKLVSIDSKAIVDGNLKLILGLIWTLILHYSISMPMWDEEEESEESKQKTPKQRLLGWIQNKLPELPITNFSRDWQSGKALGALVDSCAPGLCPDWDSWDQTKPVDNAREAMQQADDWLGVPQVITPEEIVDPNVDEHSVMTYLSQFPKAKLKPGAPLRPKLNPKKARAYGPGIEPTGNVVMKKAVFTVETISAGQGEVLVYVEDPAGHREEAKVTANNDKNRTYSVFYVPKVTGQHKVTVLFAGQHISKSPFEVDVGMAQGDSSKVTAQGPGLEPAGNIANKTTYFDVYTAGAGVGEVEVVIMDPSGKKDTVECSVEDKGNSSYRCTYKPTQEGQHIIYITFAGGQISKSPYTVNVGEACNPSLCTAKGRGLQPKGLRVKETAEFKVYTKGAGTGDLKVTIKGPKGLEEPCKKKDLGDGVYSFDYYPSTPGNYIITITWGGQHIPRSPFEVKIGSEAGPQQVRAWGPGLESGVVGKSADFVVEAVGDDVGTLGFSVEGPSPAKIECDDKGDGSCDVRYWPTEPGEYAVHVLCKNEDIQLSPFMAEIMPAPGKDFYPDKVKAYGPGLQSSGLSVGKPAEFTVDAKLGGKAPLKIQAQDRDGNPVDVQVKDNGNGTYSCSYTPRKPVKHTVMVSWGGVNIPESPFRMNIGAGCHPNKVKVSGPGVAKTGLKAYDPTYFTVDCAEAGQGDISIGIKCAAGVVGPAEADIDFDIIRNDNDTFTVKYTPPGAGSYTIMVLFADQTIPMTPIRIKVDPSHDASKVKAEGPGLSRSGVELNKPTHFTVNTKAAGKAKLDAQFTGPNKGDAVRDFDIIDNHDGTHTVKYTPVQQGNMGLNVTYGGDPIPKSPFAVAIAPSLDLSKVKVAGLGSKMTVGKDQEITVKSKGAGGQGKVGAKVTGPSAKSVPCKVEPGLSPETSQVRFIPRDKGPYEVELTYDGAPIPGSPFPIEAIAPTDPSKVRCSGPGLERAKVGEPGQFVVDCTNAGPAELTIEIISDNGTEAEVHIQDNGDGTYTITYVPLYPGAYTLTIRYGDQDVPNFPARLHVEPAVETSGVKVFGPGVEGKGVFREATTDFTVDARALTKTGGNHIKTCINNPSGNRTEALIRDLGDGTYQVEYTPYEEGTHSVEVAYDSSPVPKSPFRVPVTEGCDPARVRVHGPGLQSGITNKPNKFTVETRGAGTGGLGLAMEGPSEAKMSCTDNKDGSCCVEYIPYEPGTYNLNVTYGGQPITGSPFSVPVHDTVDPTKVKCQGQGLGNNVRANIPQVFSVDASKAGMAPLQVRVQGPKGVVEPAEVVDNGDQTHTVSYVPTREGPYSINVLYADEEIPQSPYKVKVLPTHDASKVRCSGPGLNTTGVPASLPVEFTIDAKDAGEGLLAVQITDPDGKPKKANIRDNQDGTYLVSYVPDMTGRYTILIKYGGDEIPYSPYRIRALPTGDASKCTVTVSIGGHGLGAGVGPTIQIGEQTVITVDAKAAGKGKVTCTVCTPDGGEVDVDVVENEDGTFDIFYTAPQPGKYVICVRFGGEHIPNSPFQVTALEGDSPDQLMQQSQNLQYAYAPNMGQTWATDGQLGMNGLDVAGLRPFDLVIPFTIQKGEITGDVRMPSGKVAKPDITDNKDGTVTVKYAPTEAGLHEMDIKYDGIHIPGSPLQFYVDYVNSGHVTAYGPGLIHGMVNKPAVFTVNTKDAGEGGLSLAIEGPSKADISCTDNQDGTCTVSYLPVLPGDYNIIVKYNDKNIPGSPFMSKITGDDSMRMSHLKVGSAADIPLDIGELDLSQLTATLTTPSGREEPCLLKMLRNGHVGISFVPKEIGEHLVNIKKNGRHIPSSPISVMINQSEIGDASRVRVTGQGLSEARTFEPAEFIIDTRDAGYGGLSLSIEGPSKVDINTEDQEDGTCKVTYCPTEPGNYIINIKFADQHVPGSAFTVKVTGEGRMKESITRKRRAASVANVGSQCDLSLKIPEISIADMTAQVTSPSGKVHKAEIMEGENNTYCIRFVPTEMGVHTVSVKYQGQHVPGSPFQFTVGPLGEGGAHKVRAGGPGLERAEAGVPAEFSIWTREAGAGGLSIAVEGPSKAEIAFEDRKDGSSGVSYIVQEPGDYEVSIKFNDEHIPDSPFVVPVASPSDDARRLTVASLQESGLKVNQPASFAVSLNGAKGVIDAKVHSPSGALEECCVTEIDEDKYAVRFIPRENGLYLIDVKFNGSHIPGSPFKIRVGETGQAGDPGMVSAYGAGLEGGTTGSPCEFIVNTSSAGPGALAVTIDGPSKVKMDCQECPEGYKVTYTPMAPGNYLISIKYGGPYHIVGSPFKAKITGSRLVNSHSMHETSSVLVDPVTRSMTTSQQAAPGWASSDASRVVAKGLGLNKGFIGQKNTFSVDCSKAGRNMLLVGVDGPKVPCEEILVKHLGNRLYNVSYQLKEKGEYILVVKWGDEHIPGSPYHITV

Target ORF information:

RefSeq Version	XM_021473622.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio filamin A, alpha (actin binding protein 280) (flna), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473622.1

ORF Insert Sequence:

ATGAGTCAAC ACACACGCCC CCACCAGTCG AGCGCCGCCG CTCCCCTATC CAACGCTTCT 
CTCTCCCCGG ACAAGGACGC CGACATGCCC GCTACAGAGA AAGATCTCGC CGAGGACGCG 
CCATGGAAGA AAATCCAGCA AAATACATTC ACGCGTTGGT GCAACGAGCA TCTGAAATGT 
GTCAACAAGC GCATAGCGAA CCTGCAGACG GACCTGAGCG ACGGGCTCCG GCTGATCGCG 
CTTCTGGAGG TGCTCAGCCA GAAGAAGATG TTCCGCAAGT ACAACCAGCG CCCTACCTTC 
AGACAGATGC AGCTGGAGAA CGTCTCCGTG GCGCTGGAGT TCCTGGACAT TGAAAACATC 
AAGCTGGTCT CCATTGACAG TAAGGCCATA GTGGATGGTA ACCTGAAGCT GATCCTGGGT 
CTGATATGGA CTCTTATTCT GCATTATTCC ATCTCCATGC CCATGTGGGA CGAGGAAGAG 
GAGAGCGAGG AAAGCAAGCA GAAGACGCCC AAGCAGAGGC TCCTCGGATG GATCCAGAAC 
AAGCTGCCCG AGCTGCCAAT CACCAACTTC AGCCGCGATT GGCAGTCAGG GAAAGCCCTT 
GGCGCTCTGG TGGACAGTTG TGCTCCAGGT CTGTGTCCAG ACTGGGATTC CTGGGACCAG 
ACCAAGCCTG TGGACAATGC CAGAGAGGCC ATGCAGCAGG CTGATGACTG GCTCGGTGTT 
CCTCAGGTCA TCACACCAGA AGAAATCGTT GATCCCAATG TGGACGAGCA CTCCGTTATG 
ACTTACCTGT CCCAGTTTCC CAAAGCCAAA CTCAAGCCTG GTGCCCCTCT GAGGCCCAAA 
CTCAATCCCA AAAAGGCCCG TGCTTATGGA CCAGGTATTG AGCCAACAGG TAATGTTGTG 
ATGAAGAAGG CTGTGTTCAC TGTTGAGACC ATCAGCGCTG GTCAGGGTGA GGTACTGGTT 
TATGTCGAGG ACCCCGCTGG ACACCGCGAG GAGGCCAAAG TCACAGCCAA CAATGACAAG 
AACCGCACCT ACTCAGTATT CTATGTGCCA AAAGTCACTG GACAACACAA GGTGACAGTG 
CTGTTTGCAG GGCAACACAT CTCTAAGAGC CCGTTTGAGG TGGATGTGGG AATGGCTCAG 
GGAGACTCCA GCAAGGTCAC TGCCCAGGGC CCAGGACTCG AGCCTGCAGG CAACATTGCC 
AACAAAACCA CATACTTTGA TGTCTACACA GCTGGTGCTG GAGTAGGAGA GGTGGAGGTG 
GTCATCATGG ACCCCAGTGG AAAGAAGGAC ACGGTTGAGT GCAGTGTCGA GGATAAAGGC 
AACAGCAGCT ACCGATGCAC CTACAAGCCC ACTCAGGAGG GACAGCACAT CATTTACATC 
ACCTTTGCCG GGGGACAGAT CTCCAAGAGC CCTTACACTG TCAATGTGGG AGAGGCCTGT 
AATCCAAGCC TGTGTACAGC CAAGGGCCGC GGTCTGCAGC CTAAAGGCCT GAGGGTCAAG 
GAGACCGCCG AGTTTAAGGT TTACACCAAA GGAGCAGGAA CCGGAGACCT CAAAGTTACC 
ATCAAAGGGC CCAAGGGTCT CGAGGAGCCC TGTAAGAAGA AGGATTTGGG AGACGGCGTG 
TACAGCTTTG ATTATTACCC CTCCACACCT GGAAATTACA TCATCACCAT CACATGGGGC 
GGTCAACACA TCCCACGCAG CCCGTTTGAG GTGAAGATCG GGTCTGAAGC AGGACCGCAG 
CAGGTCAGGG CGTGGGGTCC AGGACTAGAG AGTGGCGTTG TGGGCAAATC TGCTGACTTT 
GTTGTGGAAG CTGTTGGTGA TGATGTGGGC ACTTTAGGTT TCTCTGTTGA AGGCCCTTCC 
CCGGCTAAGA TTGAATGTGA TGATAAGGGA GATGGTTCAT GTGACGTGAG GTACTGGCCC 
ACCGAACCGG GTGAATATGC AGTTCATGTG CTCTGTAAGA ATGAGGACAT CCAGCTCAGC 
CCTTTCATGG CTGAAATCAT GCCCGCCCCA GGAAAAGACT TTTATCCTGA CAAGGTGAAG 
GCTTATGGCC CCGGTCTGCA GAGCTCAGGC CTGTCAGTTG GCAAACCAGC CGAGTTTACC 
GTGGATGCCA AACTGGGTGG CAAAGCTCCT CTGAAGATTC AAGCTCAGGA CCGTGATGGA 
AACCCAGTCG ATGTGCAGGT GAAGGACAAT GGAAATGGGA CATACAGCTG CAGCTACACC 
CCTCGCAAAC CAGTCAAACA CACTGTGATG GTGTCCTGGG GCGGCGTCAA CATCCCAGAG 
AGCCCGTTCA GAATGAACAT TGGGGCAGGG TGTCATCCCA ATAAGGTGAA AGTATCTGGA 
CCTGGAGTGG CCAAGACAGG CCTCAAAGCA TATGATCCAA CATACTTTAC TGTGGATTGT 
GCTGAGGCTG GACAGGGCGA CATCAGCATA GGCATCAAAT GTGCTGCAGG TGTGGTGGGA 
CCTGCTGAGG CTGACATAGA CTTCGACATC ATTAGAAATG ACAACGACAC ATTTACTGTT 
AAATACACTC CTCCTGGAGC GGGCAGCTAC ACCATCATGG TGCTGTTTGC TGATCAGACC 
ATTCCCATGA CACCCATCAG AATTAAGGTT GATCCCTCTC ATGATGCAAG CAAGGTTAAA 
GCTGAGGGGC CTGGACTTAG CCGTTCTGGT GTGGAGCTGA ACAAACCCAC TCACTTCACT 
GTGAACACAA AGGCTGCAGG CAAGGCCAAG CTTGATGCTC AGTTCACTGG ACCCAACAAG 
GGAGATGCAG TCCGTGACTT CGACATAATC GACAACCATG ATGGCACTCA CACTGTCAAA 
TATACCCCTG TCCAACAGGG CAACATGGGT TTGAATGTCA CCTACGGTGG TGACCCTATT 
CCCAAGAGCC CATTTGCAGT AGCAATTGCC CCCTCTCTGG ACCTCAGTAA AGTTAAAGTG 
GCTGGTCTTG GAAGTAAAAT GACTGTTGGA AAGGATCAGG AGATCACAGT CAAATCTAAA 
GGTGCAGGCG GTCAGGGCAA AGTTGGCGCA AAGGTTACTG GTCCATCTGC TAAATCAGTG 
CCCTGTAAAG TTGAACCAGG CCTGAGCCCA GAAACCAGCC AAGTTCGTTT TATCCCAAGA 
GATAAGGGAC CCTATGAGGT GGAACTGACA TATGATGGCG CCCCCATCCC TGGCAGTCCA 
TTCCCCATCG AAGCCATTGC ACCTACTGAC CCATCCAAGG TGCGATGCTC TGGGCCAGGA 
CTGGAGCGGG CGAAGGTTGG CGAGCCTGGA CAGTTTGTGG TGGATTGCAC TAATGCTGGC 
CCTGCTGAGT TGACCATCGA GATCATCTCT GACAATGGCA CTGAGGCTGA AGTCCACATT 
CAAGACAACG GCGACGGGAC CTACACCATC ACTTACGTCC CTCTCTACCC TGGAGCCTAT 
ACCCTCACCA TCCGCTACGG GGACCAGGAT GTGCCAAACT TCCCAGCAAG ACTCCATGTG 
GAGCCTGCTG TGGAGACCAG TGGAGTGAAA GTGTTCGGAC CCGGAGTGGA GGGCAAAGGT 
GTTTTCAGGG AAGCCACTAC AGATTTCACT GTGGATGCCC GTGCGCTCAC TAAAACCGGA 
GGCAATCACA TCAAAACCTG CATCAACAAC CCATCGGGCA ACCGCACTGA AGCACTGATC 
CGAGATCTGG GAGACGGCAC ATATCAAGTG GAGTACACGC CTTATGAGGA GGGTACACAC 
AGTGTTGAGG TTGCATATGA CAGCTCCCCG GTTCCCAAAA GCCCCTTCCG TGTGCCAGTG 
ACCGAGGGCT GTGACCCGGC GCGTGTGCGT GTGCATGGTC CAGGACTTCA GTCTGGCATC 
ACCAACAAAC CTAACAAATT TACCGTGGAG ACCCGTGGGG CTGGTACTGG TGGACTGGGG 
CTGGCTATGG AAGGACCTTC TGAGGCCAAA ATGTCCTGCA CCGATAACAA AGATGGCAGC 
TGTTGTGTTG AATACATCCC ATATGAGCCA GGGACATACA ACCTCAATGT CACCTATGGA 
GGCCAACCAA TCACTGGAAG CCCTTTCTCT GTACCTGTCC ATGATACTGT TGACCCAACT 
AAAGTGAAAT GTCAGGGCCA GGGGCTTGGA AACAACGTGC GTGCCAATAT CCCACAGGTC 
TTTTCTGTGG ATGCAAGCAA GGCTGGTATG GCGCCACTGC AGGTCAGAGT TCAGGGACCC 
AAAGGTGTTG TGGAGCCTGC TGAGGTGGTG GATAATGGTG ACCAGACTCA CACAGTCAGC 
TATGTGCCCA CAAGAGAGGG GCCATACTCC ATTAATGTTC TGTATGCTGA TGAGGAGATC 
CCACAGAGTC CTTATAAGGT GAAGGTTCTG CCCACTCATG ACGCAAGTAA AGTCCGTTGC 
AGCGGTCCTG GCCTGAATAC CACTGGTGTT CCTGCCAGTC TGCCCGTGGA GTTCACTATT 
GACGCCAAAG ATGCAGGAGA GGGACTGTTG GCCGTCCAGA TAACTGACCC TGATGGGAAG 
CCAAAGAAGG CTAACATTCG TGATAATCAG GACGGGACCT ACCTTGTGTC CTATGTGCCT 
GACATGACCG GCCGTTACAC CATTCTTATT AAGTATGGTG GTGATGAGAT CCCGTATTCT 
CCATATCGCA TTCGAGCCCT GCCTACTGGA GACGCCAGCA AGTGTACAGT CACAGTCTCA 
ATTGGAGGTC ATGGTTTGGG AGCTGGAGTT GGTCCGACTA TTCAGATTGG AGAGCAGACT 
GTCATCACTG TGGATGCGAA GGCAGCTGGG AAGGGCAAGG TGACCTGTAC CGTGTGCACA 
CCTGATGGAG GCGAGGTGGA TGTGGACGTC GTGGAAAATG AGGATGGAAC GTTTGACATC 
TTTTACACTG CACCGCAGCC TGGGAAATAC GTGATCTGTG TGCGCTTCGG TGGAGAGCAC 
ATCCCTAACA GCCCCTTCCA AGTCACGGCT TTGGAAGGTG ACTCTCCTGA CCAACTAATG 
CAGCAGAGCC AGAACCTGCA GTACGCCTAC GCGCCCAACA TGGGCCAGAC ATGGGCTACA 
GATGGGCAAT TGGGTATGAA TGGACTGGAT GTAGCTGGAC TGAGACCCTT TGACTTGGTC 
ATTCCTTTCA CCATTCAGAA AGGAGAAATT ACAGGTGATG TACGAATGCC CTCTGGAAAA 
GTGGCCAAGC CTGATATTAC AGACAACAAG GATGGCACAG TCACAGTGAA ATATGCCCCA 
ACTGAGGCGG GCCTGCATGA GATGGACATT AAATATGATG GAATACACAT TCCAGGAAGT 
CCATTGCAAT TCTACGTGGA TTATGTCAAC AGCGGTCATG TGACTGCATA TGGTCCTGGT 
CTAATTCATG GCATGGTCAA TAAGCCTGCA GTCTTCACAG TCAACACAAA GGATGCTGGA 
GAGGGTGGTC TGTCTCTGGC CATCGAGGGG CCATCAAAGG CAGATATCAG CTGCACTGAT 
AATCAGGACG GTACCTGCAC TGTTTCATAC CTCCCTGTTC TTCCCGGAGA CTACAATATC 
ATTGTTAAAT ACAATGACAA GAACATCCCC GGCAGCCCTT TCATGTCCAA AATCACAGGT 
GACGACTCGA TGCGCATGTC TCACCTGAAG GTGGGCTCAG CTGCTGACAT CCCTCTGGAC 
ATTGGTGAGC TTGACCTCAG TCAGCTGACA GCCACACTCA CCACCCCATC CGGCCGAGAG 
GAGCCTTGCC TGCTCAAGAT GCTCAGAAAT GGGCATGTGG GAATTTCCTT TGTGCCCAAG 
GAAATTGGCG AGCATCTTGT TAACATTAAG AAAAATGGAC GCCATATTCC TAGTAGCCCC 
ATTTCTGTCA TGATCAACCA ATCAGAGATC GGTGATGCCA GCCGTGTACG AGTAACTGGT 
CAAGGCCTGA GTGAAGCTCG CACCTTTGAG CCCGCAGAAT TCATCATTGA CACTAGAGAT 
GCAGGATATG GTGGACTCAG TTTGTCTATT GAGGGACCTA GTAAAGTGGA TATCAACACT 
GAAGATCAGG AAGATGGCAC CTGCAAAGTG ACATATTGCC CCACAGAGCC TGGAAACTAC 
ATAATTAACA TCAAATTTGC AGACCAGCAT GTGCCAGGAA GTGCTTTTAC TGTCAAAGTG 
ACAGGCGAGG GTCGGATGAA GGAGAGCATC ACCCGCAAAA GAAGAGCTGC TTCTGTGGCC 
AATGTTGGCA GCCAGTGCGA CCTCAGTCTA AAGATCCCTG AGATCAGCAT TGCAGACATG 
ACCGCTCAGG TGACCAGCCC ATCTGGCAAA GTCCACAAGG CAGAGATAAT GGAGGGAGAA 
AACAACACCT ACTGCATCCG ATTCGTGCCC ACAGAGATGG GTGTGCACAC AGTAAGCGTG 
AAGTATCAAG GTCAGCATGT GCCTGGATCC CCCTTCCAGT TCACGGTGGG ACCTCTTGGA 
GAAGGAGGAG CTCATAAAGT CAGAGCTGGA GGCCCAGGAT TAGAGAGGGC AGAAGCTGGT 
GTACCAGCGG AGTTCAGCAT TTGGACTCGT GAAGCAGGAG CTGGTGGTCT GTCCATCGCA 
GTGGAAGGAC CCAGCAAAGC AGAGATTGCT TTTGAAGACC GTAAAGATGG ATCCAGTGGT 
GTCTCTTACA TCGTCCAGGA GCCTGGCGAT TATGAAGTAT CCATCAAGTT CAATGACGAG 
CACATCCCTG ACAGTCCGTT TGTGGTGCCT GTGGCCTCGC CATCTGACGA TGCCCGCCGT 
CTCACTGTTG CCAGTCTTCA GGAGTCCGGG TTAAAGGTGA ACCAGCCGGC CTCTTTTGCT 
GTGAGCCTAA ATGGGGCGAA AGGAGTCATC GATGCCAAAG TTCACAGTCC ATCAGGAGCA 
CTTGAAGAGT GCTGCGTCAC AGAGATCGAT GAAGATAAAT ATGCTGTGAG ATTCATTCCC 
AGGGAGAATG GCCTGTACCT GATAGATGTG AAATTTAATG GTTCTCACAT CCCTGGAAGC 
CCCTTCAAGA TCCGTGTTGG TGAGACGGGT CAGGCTGGAG ATCCAGGAAT GGTGTCTGCT 
TATGGAGCTG GTCTGGAAGG AGGCACCACT GGATCGCCTT GTGAGTTCAT TGTAAACACC 
AGTTCAGCTG GCCCTGGAGC TTTGGCGGTG ACTATCGACG GGCCGTCCAA GGTGAAGATG 
GACTGTCAGG AATGCCCAGA GGGCTACAAA GTCACCTACA CACCTATGGC CCCTGGAAAC 
TACCTAATTT CTATTAAATA CGGTGGACCG TACCATATTG TTGGTAGTCC ATTCAAAGCT 
AAAATTACTG GCTCCCGATT GGTCAACAGC CACAGCATGC ATGAGACTTC TTCAGTACTG 
GTTGACCCTG TGACCCGCAG CATGACGACC AGCCAGCAGG CAGCGCCAGG CTGGGCCAGC 
TCTGATGCCA GCCGCGTAGT GGCCAAAGGT TTGGGGCTCA ACAAGGGCTT CATAGGACAG 
AAGAACACTT TCAGTGTGGA CTGTAGTAAA GCAGGGAGGA ACATGCTCCT GGTCGGAGTG 
GATGGACCCA AAGTGCCCTG TGAGGAGATC CTGGTGAAAC ACCTGGGCAA CCGCCTCTAC 
AACGTTTCCT ACCAGCTGAA GGAGAAAGGA GAGTACATCC TGGTGGTCAA ATGGGGTGAT 
GAGCACATCC CGGGCAGCCC GTATCACATC ACTGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

flna ( XM_021473623.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329298.1 Danio rerio filamin A, alpha (actin binding protein 280) (flna), transcript variant X2, mRNA.

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CloneID	ODa146251
Clone ID Related Accession (Same CDS sequence)	XM_021473623.1
Accession Version	XM_021473623.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	filamin-A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395142.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCAAC ACACACGCCC CCACCAGTCG AGCGCCGCCG CTCCCCTATC CAACGCTTCT 
CTCTCCCCGG ACAAGGACGC CGACATGCCC GCTACAGAGA AAGATCTCGC CGAGGACGCG 
CCATGGAAGA AAATCCAGCA AAATACATTC ACGCGTTGGT GCAACGAGCA TCTGAAATGT 
GTCAACAAGC GCATAGCGAA CCTGCAGACG GACCTGAGCG ACGGGCTCCG GCTGATCGCG 
CTTCTGGAGG TGCTCAGCCA GAAGAAGATG TTCCGCAAGT ACAACCAGCG CCCTACCTTC 
AGACAGATGC AGCTGGAGAA CGTCTCCGTG GCGCTGGAGT TCCTGGACAT TGAAAACATC 
AAGCTGGTCT CCATTGACAG TAAGGCCATA GTGGATGGTA ACCTGAAGCT GATCCTGGGT 
CTGATATGGA CTCTTATTCT GCATTATTCC ATCTCCATGC CCATGTGGGA CGAGGAAGAG 
GAGAGCGAGG AAAGCAAGCA GAAGACGCCC AAGCAGAGGC TCCTCGGATG GATCCAGAAC 
AAGCTGCCCG AGCTGCCAAT CACCAACTTC AGCCGCGATT GGCAGTCAGG GAAAGCCCTT 
GGCGCTCTGG TGGACAGTTG TGCTCCAGGT CTGTGTCCAG ACTGGGATTC CTGGGACCAG 
ACCAAGCCTG TGGACAATGC CAGAGAGGCC ATGCAGCAGG CTGATGACTG GCTCGGTGTT 
CCTCAGGTCA TCACACCAGA AGAAATCGTT GATCCCAATG TGGACGAGCA CTCCGTTATG 
ACTTACCTGT CCCAGTTTCC CAAAGCCAAA CTCAAGCCTG GTGCCCCTCT GAGGCCCAAA 
CTCAATCCCA AAAAGGCCCG TGCTTATGGA CCAGGTATTG AGCCAACAGG TAATGTTGTG 
ATGAAGAAGG CTGTGTTCAC TGTTGAGACC ATCAGCGCTG GTCAGGGTGA GGTACTGGTT 
TATGTCGAGG ACCCCGCTGG ACACCGCGAG GAGGCCAAAG TCACAGCCAA CAATGACAAG 
AACCGCACCT ACTCAGTATT CTATGTGCCA AAAGTCACTG GACAACACAA GGTGACAGTG 
CTGTTTGCAG GGCAACACAT CTCTAAGAGC CCGTTTGAGG TGGATGTGGG AATGGCTCAG 
GGAGACTCCA GCAAGGTCAC TGCCCAGGGC CCAGGACTCG AGCCTGCAGG CAACATTGCC 
AACAAAACCA CATACTTTGA TGTCTACACA GCTGGTGCTG GAGTAGGAGA GGTGGAGGTG 
GTCATCATGG ACCCCAGTGG AAAGAAGGAC ACGGTTGAGT GCAGTGTCGA GGATAAAGGC 
AACAGCAGCT ACCGATGCAC CTACAAGCCC ACTCAGGAGG GACAGCACAT CATTTACATC 
ACCTTTGCCG GGGGACAGAT CTCCAAGAGC CCTTACACTG TCAATGTGGG AGAGGCCTGT 
AATCCAAGCC TGTGTACAGC CAAGGGCCGC GGTCTGCAGC CTAAAGGCCT GAGGGTCAAG 
GAGACCGCCG AGTTTAAGGT TTACACCAAA GGAGCAGGAA CCGGAGACCT CAAAGTTACC 
ATCAAAGGGC CCAAGGGTCT CGAGGAGCCC TGTAAGAAGA AGGATTTGGG AGACGGCGTG 
TACAGCTTTG ATTATTACCC CTCCACACCT GGAAATTACA TCATCACCAT CACATGGGGC 
GGTCAACACA TCCCACGCAG CCCGTTTGAG GTGAAGATCG GGTCTGAAGC AGGACCGCAG 
CAGGTCAGGG CGTGGGGTCC AGGACTAGAG AGTGGCGTTG TGGGCAAATC TGCTGACTTT 
GTTGTGGAAG CTGTTGGTGA TGATGTGGGC ACTTTAGGTT TCTCTGTTGA AGGCCCTTCC 
CCGGCTAAGA TTGAATGTGA TGATAAGGGA GATGGTTCAT GTGACGTGAG GTACTGGCCC 
ACCGAACCGG GTGAATATGC AGTTCATGTG CTCTGTAAGA ATGAGGACAT CCAGCTCAGC 
CCTTTCATGG CTGAAATCAT GCCCGCCCCA GGAAAAGACT TTTATCCTGA CAAGGTGAAG 
GCTTATGGCC CCGGTCTGCA GAGCTCAGGC CTGTCAGTTG GCAAACCAGC CGAGTTTACC 
GTGGATGCCA AACTGGGTGG CAAAGCTCCT CTGAAGATTC AAGCTCAGGA CCGTGATGGA 
AACCCAGTCG ATGTGCAGGT GAAGGACAAT GGAAATGGGA CATACAGCTG CAGCTACACC 
CCTCGCAAAC CAGTCAAACA CACTGTGATG GTGTCCTGGG GCGGCGTCAA CATCCCAGAG 
AGCCCGTTCA GAATGAACAT TGGGGCAGGG TGTCATCCCA ATAAGGTGAA AGTATCTGGA 
CCTGGAGTGG CCAAGACAGG CCTCAAAGCA TATGATCCAA CATACTTTAC TGTGGATTGT 
GCTGAGGCTG GACAGGGCGA CATCAGCATA GGCATCAAAT GTGCTGCAGG TGTGGTGGGA 
CCTGCTGAGG CTGACATAGA CTTCGACATC ATTAGAAATG ACAACGACAC ATTTACTGTT 
AAATACACTC CTCCTGGAGC GGGCAGCTAC ACCATCATGG TGCTGTTTGC TGATCAGACC 
ATTCCCATGA CACCCATCAG AATTAAGGTT GATCCCTCTC ATGATGCAAG CAAGGTTAAA 
GCTGAGGGGC CTGGACTTAG CCGTTCTGGT GTGGAGCTGA ACAAACCCAC TCACTTCACT 
GTGAACACAA AGGCTGCAGG CAAGGCCAAG CTTGATGCTC AGTTCACTGG ACCCAACAAG 
GGAGATGCAG TCCGTGACTT CGACATAATC GACAACCATG ATGGCACTCA CACTGTCAAA 
TATACCCCTG TCCAACAGGG CAACATGGGT TTGAATGTCA CCTACGGTGG TGACCCTATT 
CCCAAGAGCC CATTTGCAGT AGCAATTGCC CCCTCTCTGG ACCTCAGTAA AGTTAAAGTG 
GCTGGTCTTG GAAGTAAAAT GACTGTTGGA AAGGATCAGG AGATCACAGT CAAATCTAAA 
GGTGCAGGCG GTCAGGGCAA AGTTGGCGCA AAGGTTACTG GTCCATCTGC TAAATCAGTG 
CCCTGTAAAG TTGAACCAGG CCTGAGCCCA GAAACCAGCC AAGTTCGTTT TATCCCAAGA 
GATAAGGGAC CCTATGAGGT GGAACTGACA TATGATGGCG CCCCCATCCC TGGCAGTCCA 
TTCCCCATCG AAGCCATTGC ACCTACTGAC CCATCCAAGG TGCGATGCTC TGGGCCAGGA 
CTGGAGCGGG CGAAGGTTGG CGAGCCTGGA CAGTTTGTGG TGGATTGCAC TAATGCTGGC 
CCTGCTGAGT TGACCATCGA GATCATCTCT GACAATGGCA CTGAGGCTGA AGTCCACATT 
CAAGACAACG GCGACGGGAC CTACACCATC ACTTACGTCC CTCTCTACCC TGGAGCCTAT 
ACCCTCACCA TCCGCTACGG GGACCAGGAT GTGCCAAACT TCCCAGCAAG ACTCCATGTG 
GAGCCTGCTG TGGAGACCAG TGGAGTGAAA GTGTTCGGAC CCGGAGTGGA GGGCAAAGGT 
GTTTTCAGGG AAGCCACTAC AGATTTCACT GTGGATGCCC GTGCGCTCAC TAAAACCGGA 
GGCAATCACA TCAAAACCTG CATCAACAAC CCATCGGGCA ACCGCACTGA AGCACTGATC 
CGAGATCTGG GAGACGGCAC ATATCAAGTG GAGTACACGC CTTATGAGGA GGGTACACAC 
AGTGTTGAGG TTGCATATGA CAGCTCCCCG GTTCCCAAAA GCCCCTTCCG TGTGCCAGTG 
ACCGAGGGCT GTGACCCGGC GCGTGTGCGT GTGCATGGTC CAGGACTTCA GTCTGGCATC 
ACCAACAAAC CTAACAAATT TACCGTGGAG ACCCGTGGGG CTGGTACTGG TGGACTGGGG 
CTGGCTATGG AAGGACCTTC TGAGGCCAAA ATGTCCTGCA CCGATAACAA AGATGGCAGC 
TGTTGTGTTG AATACATCCC ATATGAGCCA GGGACATACA ACCTCAATGT CACCTATGGA 
GGCCAACCAA TCACTGGAAG CCCTTTCTCT GTACCTGTCC ATGATACTGT TGACCCAACT 
AAAGTGAAAT GTCAGGGCCA GGGGCTTGGA AACAACGTGC GTGCCAATAT CCCACAGGTC 
TTTTCTGTGG ATGCAAGCAA GGCTGGTATG GCGCCACTGC AGGTCAGAGT TCAGGGACCC 
AAAGGTGTTG TGGAGCCTGC TGAGGTGGTG GATAATGGTG ACCAGACTCA CACAGTCAGC 
TATGTGCCCA CAAGAGAGGG GCCATACTCC ATTAATGTTC TGTATGCTGA TGAGGAGATC 
CCACAGAGTC CTTATAAGGT GAAGGTTCTG CCCACTCATG ACGCAAGTAA AGTCCGTTGC 
AGCGGTCCTG GCCTGAATAC CACTGGTGTT CCTGCCAGTC TGCCCGTGGA GTTCACTATT 
GACGCCAAAG ATGCAGGAGA GGGACTGTTG GCCGTCCAGA TAACTGACCC TGATGGGAAG 
CCAAAGAAGG CTAACATTCG TGATAATCAG GACGGGACCT ACCTTGTGTC CTATGTGCCT 
GACATGACCG GCCGTTACAC CATTCTTATT AAGTATGGTG GTGATGAGAT CCCGTATTCT 
CCATATCGCA TTCGAGCCCT GCCTACTGGA GACGCCAGCA AGTGTACAGT CACAGGAGCT 
GGAGTTGGTC CGACTATTCA GATTGGAGAG CAGACTGTCA TCACTGTGGA TGCGAAGGCA 
GCTGGGAAGG GCAAGGTGAC CTGTACCGTG TGCACACCTG ATGGAGGCGA GGTGGATGTG 
GACGTCGTGG AAAATGAGGA TGGAACGTTT GACATCTTTT ACACTGCACC GCAGCCTGGG 
AAATACGTGA TCTGTGTGCG CTTCGGTGGA GAGCACATCC CTAACAGCCC CTTCCAAGTC 
ACGGCTTTGG AAGGTGACTC TCCTGACCAA CTAATGCAGC AGAGCCAGAA CCTGCAGTAC 
GCCTACGCGC CCAACATGGG CCAGACATGG GCTACAGATG GGCAATTGGG TATGAATGGA 
CTGGATGTAG CTGGACTGAG ACCCTTTGAC TTGGTCATTC CTTTCACCAT TCAGAAAGGA 
GAAATTACAG GTGATGTACG AATGCCCTCT GGAAAAGTGG CCAAGCCTGA TATTACAGAC 
AACAAGGATG GCACAGTCAC AGTGAAATAT GCCCCAACTG AGGCGGGCCT GCATGAGATG 
GACATTAAAT ATGATGGAAT ACACATTCCA GGAAGTCCAT TGCAATTCTA CGTGGATTAT 
GTCAACAGCG GTCATGTGAC TGCATATGGT CCTGGTCTAA TTCATGGCAT GGTCAATAAG 
CCTGCAGTCT TCACAGTCAA CACAAAGGAT GCTGGAGAGG GTGGTCTGTC TCTGGCCATC 
GAGGGGCCAT CAAAGGCAGA TATCAGCTGC ACTGATAATC AGGACGGTAC CTGCACTGTT 
TCATACCTCC CTGTTCTTCC CGGAGACTAC AATATCATTG TTAAATACAA TGACAAGAAC 
ATCCCCGGCA GCCCTTTCAT GTCCAAAATC ACAGGTGACG ACTCGATGCG CATGTCTCAC 
CTGAAGGTGG GCTCAGCTGC TGACATCCCT CTGGACATTG GTGAGCTTGA CCTCAGTCAG 
CTGACAGCCA CACTCACCAC CCCATCCGGC CGAGAGGAGC CTTGCCTGCT CAAGATGCTC 
AGAAATGGGC ATGTGGGAAT TTCCTTTGTG CCCAAGGAAA TTGGCGAGCA TCTTGTTAAC 
ATTAAGAAAA ATGGACGCCA TATTCCTAGT AGCCCCATTT CTGTCATGAT CAACCAATCA 
GAGATCGGTG ATGCCAGCCG TGTACGAGTA ACTGGTCAAG GCCTGAGTGA AGCTCGCACC 
TTTGAGCCCG CAGAATTCAT CATTGACACT AGAGATGCAG GATATGGTGG ACTCAGTTTG 
TCTATTGAGG GACCTAGTAA AGTGGATATC AACACTGAAG ATCAGGAAGA TGGCACCTGC 
AAAGTGACAT ATTGCCCCAC AGAGCCTGGA AACTACATAA TTAACATCAA ATTTGCAGAC 
CAGCATGTGC CAGGAAGTGC TTTTACTGTC AAAGTGACAG GCGAGGGTCG GATGAAGGAG 
AGCATCACCC GCAAAAGAAG AGCTGCTTCT GTGGCCAATG TTGGCAGCCA GTGCGACCTC 
AGTCTAAAGA TCCCTGAGAT CAGCATTGCA GACATGACCG CTCAGGTGAC CAGCCCATCT 
GGCAAAGTCC ACAAGGCAGA GATAATGGAG GGAGAAAACA ACACCTACTG CATCCGATTC 
GTGCCCACAG AGATGGGTGT GCACACAGTA AGCGTGAAGT ATCAAGGTCA GCATGTGCCT 
GGATCCCCCT TCCAGTTCAC GGTGGGACCT CTTGGAGAAG GAGGAGCTCA TAAAGTCAGA 
GCTGGAGGCC CAGGATTAGA GAGGGCAGAA GCTGGTGTAC CAGCGGAGTT CAGCATTTGG 
ACTCGTGAAG CAGGAGCTGG TGGTCTGTCC ATCGCAGTGG AAGGACCCAG CAAAGCAGAG 
ATTGCTTTTG AAGACCGTAA AGATGGATCC AGTGGTGTCT CTTACATCGT CCAGGAGCCT 
GGCGATTATG AAGTATCCAT CAAGTTCAAT GACGAGCACA TCCCTGACAG TCCGTTTGTG 
GTGCCTGTGG CCTCGCCATC TGACGATGCC CGCCGTCTCA CTGTTGCCAG TCTTCAGGAG 
TCCGGGTTAA AGGTGAACCA GCCGGCCTCT TTTGCTGTGA GCCTAAATGG GGCGAAAGGA 
GTCATCGATG CCAAAGTTCA CAGTCCATCA GGAGCACTTG AAGAGTGCTG CGTCACAGAG 
ATCGATGAAG ATAAATATGC TGTGAGATTC ATTCCCAGGG AGAATGGCCT GTACCTGATA 
GATGTGAAAT TTAATGGTTC TCACATCCCT GGAAGCCCCT TCAAGATCCG TGTTGGTGAG 
ACGGGTCAGG CTGGAGATCC AGGAATGGTG TCTGCTTATG GAGCTGGTCT GGAAGGAGGC 
ACCACTGGAT CGCCTTGTGA GTTCATTGTA AACACCAGTT CAGCTGGCCC TGGAGCTTTG 
GCGGTGACTA TCGACGGGCC GTCCAAGGTG AAGATGGACT GTCAGGAATG CCCAGAGGGC 
TACAAAGTCA CCTACACACC TATGGCCCCT GGAAACTACC TAATTTCTAT TAAATACGGT 
GGACCGTACC ATATTGTTGG TAGTCCATTC AAAGCTAAAA TTACTGGCTC CCGATTGGTC 
AACAGCCACA GCATGCATGA GACTTCTTCA GTACTGGTTG ACCCTGTGAC CCGCAGCATG 
ACGACCAGCC AGCAGGCAGC GCCAGGCTGG GCCAGCTCTG ATGCCAGCCG CGTAGTGGCC 
AAAGGTTTGG GGCTCAACAA GGGCTTCATA GGACAGAAGA ACACTTTCAG TGTGGACTGT 
AGTAAAGCAG GGAGGAACAT GCTCCTGGTC GGAGTGGATG GACCCAAAGT GCCCTGTGAG 
GAGATCCTGG TGAAACACCT GGGCAACCGC CTCTACAACG TTTCCTACCA GCTGAAGGAG 
AAAGGAGAGT ACATCCTGGT GGTCAAATGG GGTGATGAGC ACATCCCGGG CAGCCCGTAT 
CACATCACTG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329298.1
CDS	352..7986
Translation	MSQHTRPHQSSAAAPLSNASLSPDKDADMPATEKDLAEDAPWKKIQQNTFTRWCNEHLKCVNKRIANLQTDLSDGLRLIALLEVLSQKKMFRKYNQRPTFRQMQLENVSVALEFLDIENIKLVSIDSKAIVDGNLKLILGLIWTLILHYSISMPMWDEEEESEESKQKTPKQRLLGWIQNKLPELPITNFSRDWQSGKALGALVDSCAPGLCPDWDSWDQTKPVDNAREAMQQADDWLGVPQVITPEEIVDPNVDEHSVMTYLSQFPKAKLKPGAPLRPKLNPKKARAYGPGIEPTGNVVMKKAVFTVETISAGQGEVLVYVEDPAGHREEAKVTANNDKNRTYSVFYVPKVTGQHKVTVLFAGQHISKSPFEVDVGMAQGDSSKVTAQGPGLEPAGNIANKTTYFDVYTAGAGVGEVEVVIMDPSGKKDTVECSVEDKGNSSYRCTYKPTQEGQHIIYITFAGGQISKSPYTVNVGEACNPSLCTAKGRGLQPKGLRVKETAEFKVYTKGAGTGDLKVTIKGPKGLEEPCKKKDLGDGVYSFDYYPSTPGNYIITITWGGQHIPRSPFEVKIGSEAGPQQVRAWGPGLESGVVGKSADFVVEAVGDDVGTLGFSVEGPSPAKIECDDKGDGSCDVRYWPTEPGEYAVHVLCKNEDIQLSPFMAEIMPAPGKDFYPDKVKAYGPGLQSSGLSVGKPAEFTVDAKLGGKAPLKIQAQDRDGNPVDVQVKDNGNGTYSCSYTPRKPVKHTVMVSWGGVNIPESPFRMNIGAGCHPNKVKVSGPGVAKTGLKAYDPTYFTVDCAEAGQGDISIGIKCAAGVVGPAEADIDFDIIRNDNDTFTVKYTPPGAGSYTIMVLFADQTIPMTPIRIKVDPSHDASKVKAEGPGLSRSGVELNKPTHFTVNTKAAGKAKLDAQFTGPNKGDAVRDFDIIDNHDGTHTVKYTPVQQGNMGLNVTYGGDPIPKSPFAVAIAPSLDLSKVKVAGLGSKMTVGKDQEITVKSKGAGGQGKVGAKVTGPSAKSVPCKVEPGLSPETSQVRFIPRDKGPYEVELTYDGAPIPGSPFPIEAIAPTDPSKVRCSGPGLERAKVGEPGQFVVDCTNAGPAELTIEIISDNGTEAEVHIQDNGDGTYTITYVPLYPGAYTLTIRYGDQDVPNFPARLHVEPAVETSGVKVFGPGVEGKGVFREATTDFTVDARALTKTGGNHIKTCINNPSGNRTEALIRDLGDGTYQVEYTPYEEGTHSVEVAYDSSPVPKSPFRVPVTEGCDPARVRVHGPGLQSGITNKPNKFTVETRGAGTGGLGLAMEGPSEAKMSCTDNKDGSCCVEYIPYEPGTYNLNVTYGGQPITGSPFSVPVHDTVDPTKVKCQGQGLGNNVRANIPQVFSVDASKAGMAPLQVRVQGPKGVVEPAEVVDNGDQTHTVSYVPTREGPYSINVLYADEEIPQSPYKVKVLPTHDASKVRCSGPGLNTTGVPASLPVEFTIDAKDAGEGLLAVQITDPDGKPKKANIRDNQDGTYLVSYVPDMTGRYTILIKYGGDEIPYSPYRIRALPTGDASKCTVTGAGVGPTIQIGEQTVITVDAKAAGKGKVTCTVCTPDGGEVDVDVVENEDGTFDIFYTAPQPGKYVICVRFGGEHIPNSPFQVTALEGDSPDQLMQQSQNLQYAYAPNMGQTWATDGQLGMNGLDVAGLRPFDLVIPFTIQKGEITGDVRMPSGKVAKPDITDNKDGTVTVKYAPTEAGLHEMDIKYDGIHIPGSPLQFYVDYVNSGHVTAYGPGLIHGMVNKPAVFTVNTKDAGEGGLSLAIEGPSKADISCTDNQDGTCTVSYLPVLPGDYNIIVKYNDKNIPGSPFMSKITGDDSMRMSHLKVGSAADIPLDIGELDLSQLTATLTTPSGREEPCLLKMLRNGHVGISFVPKEIGEHLVNIKKNGRHIPSSPISVMINQSEIGDASRVRVTGQGLSEARTFEPAEFIIDTRDAGYGGLSLSIEGPSKVDINTEDQEDGTCKVTYCPTEPGNYIINIKFADQHVPGSAFTVKVTGEGRMKESITRKRRAASVANVGSQCDLSLKIPEISIADMTAQVTSPSGKVHKAEIMEGENNTYCIRFVPTEMGVHTVSVKYQGQHVPGSPFQFTVGPLGEGGAHKVRAGGPGLERAEAGVPAEFSIWTREAGAGGLSIAVEGPSKAEIAFEDRKDGSSGVSYIVQEPGDYEVSIKFNDEHIPDSPFVVPVASPSDDARRLTVASLQESGLKVNQPASFAVSLNGAKGVIDAKVHSPSGALEECCVTEIDEDKYAVRFIPRENGLYLIDVKFNGSHIPGSPFKIRVGETGQAGDPGMVSAYGAGLEGGTTGSPCEFIVNTSSAGPGALAVTIDGPSKVKMDCQECPEGYKVTYTPMAPGNYLISIKYGGPYHIVGSPFKAKITGSRLVNSHSMHETSSVLVDPVTRSMTTSQQAAPGWASSDASRVVAKGLGLNKGFIGQKNTFSVDCSKAGRNMLLVGVDGPKVPCEEILVKHLGNRLYNVSYQLKEKGEYILVVKWGDEHIPGSPYHITV

Target ORF information:

RefSeq Version	XM_021473623.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio filamin A, alpha (actin binding protein 280) (flna), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473623.1

ORF Insert Sequence:

ATGAGTCAAC ACACACGCCC CCACCAGTCG AGCGCCGCCG CTCCCCTATC CAACGCTTCT 
CTCTCCCCGG ACAAGGACGC CGACATGCCC GCTACAGAGA AAGATCTCGC CGAGGACGCG 
CCATGGAAGA AAATCCAGCA AAATACATTC ACGCGTTGGT GCAACGAGCA TCTGAAATGT 
GTCAACAAGC GCATAGCGAA CCTGCAGACG GACCTGAGCG ACGGGCTCCG GCTGATCGCG 
CTTCTGGAGG TGCTCAGCCA GAAGAAGATG TTCCGCAAGT ACAACCAGCG CCCTACCTTC 
AGACAGATGC AGCTGGAGAA CGTCTCCGTG GCGCTGGAGT TCCTGGACAT TGAAAACATC 
AAGCTGGTCT CCATTGACAG TAAGGCCATA GTGGATGGTA ACCTGAAGCT GATCCTGGGT 
CTGATATGGA CTCTTATTCT GCATTATTCC ATCTCCATGC CCATGTGGGA CGAGGAAGAG 
GAGAGCGAGG AAAGCAAGCA GAAGACGCCC AAGCAGAGGC TCCTCGGATG GATCCAGAAC 
AAGCTGCCCG AGCTGCCAAT CACCAACTTC AGCCGCGATT GGCAGTCAGG GAAAGCCCTT 
GGCGCTCTGG TGGACAGTTG TGCTCCAGGT CTGTGTCCAG ACTGGGATTC CTGGGACCAG 
ACCAAGCCTG TGGACAATGC CAGAGAGGCC ATGCAGCAGG CTGATGACTG GCTCGGTGTT 
CCTCAGGTCA TCACACCAGA AGAAATCGTT GATCCCAATG TGGACGAGCA CTCCGTTATG 
ACTTACCTGT CCCAGTTTCC CAAAGCCAAA CTCAAGCCTG GTGCCCCTCT GAGGCCCAAA 
CTCAATCCCA AAAAGGCCCG TGCTTATGGA CCAGGTATTG AGCCAACAGG TAATGTTGTG 
ATGAAGAAGG CTGTGTTCAC TGTTGAGACC ATCAGCGCTG GTCAGGGTGA GGTACTGGTT 
TATGTCGAGG ACCCCGCTGG ACACCGCGAG GAGGCCAAAG TCACAGCCAA CAATGACAAG 
AACCGCACCT ACTCAGTATT CTATGTGCCA AAAGTCACTG GACAACACAA GGTGACAGTG 
CTGTTTGCAG GGCAACACAT CTCTAAGAGC CCGTTTGAGG TGGATGTGGG AATGGCTCAG 
GGAGACTCCA GCAAGGTCAC TGCCCAGGGC CCAGGACTCG AGCCTGCAGG CAACATTGCC 
AACAAAACCA CATACTTTGA TGTCTACACA GCTGGTGCTG GAGTAGGAGA GGTGGAGGTG 
GTCATCATGG ACCCCAGTGG AAAGAAGGAC ACGGTTGAGT GCAGTGTCGA GGATAAAGGC 
AACAGCAGCT ACCGATGCAC CTACAAGCCC ACTCAGGAGG GACAGCACAT CATTTACATC 
ACCTTTGCCG GGGGACAGAT CTCCAAGAGC CCTTACACTG TCAATGTGGG AGAGGCCTGT 
AATCCAAGCC TGTGTACAGC CAAGGGCCGC GGTCTGCAGC CTAAAGGCCT GAGGGTCAAG 
GAGACCGCCG AGTTTAAGGT TTACACCAAA GGAGCAGGAA CCGGAGACCT CAAAGTTACC 
ATCAAAGGGC CCAAGGGTCT CGAGGAGCCC TGTAAGAAGA AGGATTTGGG AGACGGCGTG 
TACAGCTTTG ATTATTACCC CTCCACACCT GGAAATTACA TCATCACCAT CACATGGGGC 
GGTCAACACA TCCCACGCAG CCCGTTTGAG GTGAAGATCG GGTCTGAAGC AGGACCGCAG 
CAGGTCAGGG CGTGGGGTCC AGGACTAGAG AGTGGCGTTG TGGGCAAATC TGCTGACTTT 
GTTGTGGAAG CTGTTGGTGA TGATGTGGGC ACTTTAGGTT TCTCTGTTGA AGGCCCTTCC 
CCGGCTAAGA TTGAATGTGA TGATAAGGGA GATGGTTCAT GTGACGTGAG GTACTGGCCC 
ACCGAACCGG GTGAATATGC AGTTCATGTG CTCTGTAAGA ATGAGGACAT CCAGCTCAGC 
CCTTTCATGG CTGAAATCAT GCCCGCCCCA GGAAAAGACT TTTATCCTGA CAAGGTGAAG 
GCTTATGGCC CCGGTCTGCA GAGCTCAGGC CTGTCAGTTG GCAAACCAGC CGAGTTTACC 
GTGGATGCCA AACTGGGTGG CAAAGCTCCT CTGAAGATTC AAGCTCAGGA CCGTGATGGA 
AACCCAGTCG ATGTGCAGGT GAAGGACAAT GGAAATGGGA CATACAGCTG CAGCTACACC 
CCTCGCAAAC CAGTCAAACA CACTGTGATG GTGTCCTGGG GCGGCGTCAA CATCCCAGAG 
AGCCCGTTCA GAATGAACAT TGGGGCAGGG TGTCATCCCA ATAAGGTGAA AGTATCTGGA 
CCTGGAGTGG CCAAGACAGG CCTCAAAGCA TATGATCCAA CATACTTTAC TGTGGATTGT 
GCTGAGGCTG GACAGGGCGA CATCAGCATA GGCATCAAAT GTGCTGCAGG TGTGGTGGGA 
CCTGCTGAGG CTGACATAGA CTTCGACATC ATTAGAAATG ACAACGACAC ATTTACTGTT 
AAATACACTC CTCCTGGAGC GGGCAGCTAC ACCATCATGG TGCTGTTTGC TGATCAGACC 
ATTCCCATGA CACCCATCAG AATTAAGGTT GATCCCTCTC ATGATGCAAG CAAGGTTAAA 
GCTGAGGGGC CTGGACTTAG CCGTTCTGGT GTGGAGCTGA ACAAACCCAC TCACTTCACT 
GTGAACACAA AGGCTGCAGG CAAGGCCAAG CTTGATGCTC AGTTCACTGG ACCCAACAAG 
GGAGATGCAG TCCGTGACTT CGACATAATC GACAACCATG ATGGCACTCA CACTGTCAAA 
TATACCCCTG TCCAACAGGG CAACATGGGT TTGAATGTCA CCTACGGTGG TGACCCTATT 
CCCAAGAGCC CATTTGCAGT AGCAATTGCC CCCTCTCTGG ACCTCAGTAA AGTTAAAGTG 
GCTGGTCTTG GAAGTAAAAT GACTGTTGGA AAGGATCAGG AGATCACAGT CAAATCTAAA 
GGTGCAGGCG GTCAGGGCAA AGTTGGCGCA AAGGTTACTG GTCCATCTGC TAAATCAGTG 
CCCTGTAAAG TTGAACCAGG CCTGAGCCCA GAAACCAGCC AAGTTCGTTT TATCCCAAGA 
GATAAGGGAC CCTATGAGGT GGAACTGACA TATGATGGCG CCCCCATCCC TGGCAGTCCA 
TTCCCCATCG AAGCCATTGC ACCTACTGAC CCATCCAAGG TGCGATGCTC TGGGCCAGGA 
CTGGAGCGGG CGAAGGTTGG CGAGCCTGGA CAGTTTGTGG TGGATTGCAC TAATGCTGGC 
CCTGCTGAGT TGACCATCGA GATCATCTCT GACAATGGCA CTGAGGCTGA AGTCCACATT 
CAAGACAACG GCGACGGGAC CTACACCATC ACTTACGTCC CTCTCTACCC TGGAGCCTAT 
ACCCTCACCA TCCGCTACGG GGACCAGGAT GTGCCAAACT TCCCAGCAAG ACTCCATGTG 
GAGCCTGCTG TGGAGACCAG TGGAGTGAAA GTGTTCGGAC CCGGAGTGGA GGGCAAAGGT 
GTTTTCAGGG AAGCCACTAC AGATTTCACT GTGGATGCCC GTGCGCTCAC TAAAACCGGA 
GGCAATCACA TCAAAACCTG CATCAACAAC CCATCGGGCA ACCGCACTGA AGCACTGATC 
CGAGATCTGG GAGACGGCAC ATATCAAGTG GAGTACACGC CTTATGAGGA GGGTACACAC 
AGTGTTGAGG TTGCATATGA CAGCTCCCCG GTTCCCAAAA GCCCCTTCCG TGTGCCAGTG 
ACCGAGGGCT GTGACCCGGC GCGTGTGCGT GTGCATGGTC CAGGACTTCA GTCTGGCATC 
ACCAACAAAC CTAACAAATT TACCGTGGAG ACCCGTGGGG CTGGTACTGG TGGACTGGGG 
CTGGCTATGG AAGGACCTTC TGAGGCCAAA ATGTCCTGCA CCGATAACAA AGATGGCAGC 
TGTTGTGTTG AATACATCCC ATATGAGCCA GGGACATACA ACCTCAATGT CACCTATGGA 
GGCCAACCAA TCACTGGAAG CCCTTTCTCT GTACCTGTCC ATGATACTGT TGACCCAACT 
AAAGTGAAAT GTCAGGGCCA GGGGCTTGGA AACAACGTGC GTGCCAATAT CCCACAGGTC 
TTTTCTGTGG ATGCAAGCAA GGCTGGTATG GCGCCACTGC AGGTCAGAGT TCAGGGACCC 
AAAGGTGTTG TGGAGCCTGC TGAGGTGGTG GATAATGGTG ACCAGACTCA CACAGTCAGC 
TATGTGCCCA CAAGAGAGGG GCCATACTCC ATTAATGTTC TGTATGCTGA TGAGGAGATC 
CCACAGAGTC CTTATAAGGT GAAGGTTCTG CCCACTCATG ACGCAAGTAA AGTCCGTTGC 
AGCGGTCCTG GCCTGAATAC CACTGGTGTT CCTGCCAGTC TGCCCGTGGA GTTCACTATT 
GACGCCAAAG ATGCAGGAGA GGGACTGTTG GCCGTCCAGA TAACTGACCC TGATGGGAAG 
CCAAAGAAGG CTAACATTCG TGATAATCAG GACGGGACCT ACCTTGTGTC CTATGTGCCT 
GACATGACCG GCCGTTACAC CATTCTTATT AAGTATGGTG GTGATGAGAT CCCGTATTCT 
CCATATCGCA TTCGAGCCCT GCCTACTGGA GACGCCAGCA AGTGTACAGT CACAGGAGCT 
GGAGTTGGTC CGACTATTCA GATTGGAGAG CAGACTGTCA TCACTGTGGA TGCGAAGGCA 
GCTGGGAAGG GCAAGGTGAC CTGTACCGTG TGCACACCTG ATGGAGGCGA GGTGGATGTG 
GACGTCGTGG AAAATGAGGA TGGAACGTTT GACATCTTTT ACACTGCACC GCAGCCTGGG 
AAATACGTGA TCTGTGTGCG CTTCGGTGGA GAGCACATCC CTAACAGCCC CTTCCAAGTC 
ACGGCTTTGG AAGGTGACTC TCCTGACCAA CTAATGCAGC AGAGCCAGAA CCTGCAGTAC 
GCCTACGCGC CCAACATGGG CCAGACATGG GCTACAGATG GGCAATTGGG TATGAATGGA 
CTGGATGTAG CTGGACTGAG ACCCTTTGAC TTGGTCATTC CTTTCACCAT TCAGAAAGGA 
GAAATTACAG GTGATGTACG AATGCCCTCT GGAAAAGTGG CCAAGCCTGA TATTACAGAC 
AACAAGGATG GCACAGTCAC AGTGAAATAT GCCCCAACTG AGGCGGGCCT GCATGAGATG 
GACATTAAAT ATGATGGAAT ACACATTCCA GGAAGTCCAT TGCAATTCTA CGTGGATTAT 
GTCAACAGCG GTCATGTGAC TGCATATGGT CCTGGTCTAA TTCATGGCAT GGTCAATAAG 
CCTGCAGTCT TCACAGTCAA CACAAAGGAT GCTGGAGAGG GTGGTCTGTC TCTGGCCATC 
GAGGGGCCAT CAAAGGCAGA TATCAGCTGC ACTGATAATC AGGACGGTAC CTGCACTGTT 
TCATACCTCC CTGTTCTTCC CGGAGACTAC AATATCATTG TTAAATACAA TGACAAGAAC 
ATCCCCGGCA GCCCTTTCAT GTCCAAAATC ACAGGTGACG ACTCGATGCG CATGTCTCAC 
CTGAAGGTGG GCTCAGCTGC TGACATCCCT CTGGACATTG GTGAGCTTGA CCTCAGTCAG 
CTGACAGCCA CACTCACCAC CCCATCCGGC CGAGAGGAGC CTTGCCTGCT CAAGATGCTC 
AGAAATGGGC ATGTGGGAAT TTCCTTTGTG CCCAAGGAAA TTGGCGAGCA TCTTGTTAAC 
ATTAAGAAAA ATGGACGCCA TATTCCTAGT AGCCCCATTT CTGTCATGAT CAACCAATCA 
GAGATCGGTG ATGCCAGCCG TGTACGAGTA ACTGGTCAAG GCCTGAGTGA AGCTCGCACC 
TTTGAGCCCG CAGAATTCAT CATTGACACT AGAGATGCAG GATATGGTGG ACTCAGTTTG 
TCTATTGAGG GACCTAGTAA AGTGGATATC AACACTGAAG ATCAGGAAGA TGGCACCTGC 
AAAGTGACAT ATTGCCCCAC AGAGCCTGGA AACTACATAA TTAACATCAA ATTTGCAGAC 
CAGCATGTGC CAGGAAGTGC TTTTACTGTC AAAGTGACAG GCGAGGGTCG GATGAAGGAG 
AGCATCACCC GCAAAAGAAG AGCTGCTTCT GTGGCCAATG TTGGCAGCCA GTGCGACCTC 
AGTCTAAAGA TCCCTGAGAT CAGCATTGCA GACATGACCG CTCAGGTGAC CAGCCCATCT 
GGCAAAGTCC ACAAGGCAGA GATAATGGAG GGAGAAAACA ACACCTACTG CATCCGATTC 
GTGCCCACAG AGATGGGTGT GCACACAGTA AGCGTGAAGT ATCAAGGTCA GCATGTGCCT 
GGATCCCCCT TCCAGTTCAC GGTGGGACCT CTTGGAGAAG GAGGAGCTCA TAAAGTCAGA 
GCTGGAGGCC CAGGATTAGA GAGGGCAGAA GCTGGTGTAC CAGCGGAGTT CAGCATTTGG 
ACTCGTGAAG CAGGAGCTGG TGGTCTGTCC ATCGCAGTGG AAGGACCCAG CAAAGCAGAG 
ATTGCTTTTG AAGACCGTAA AGATGGATCC AGTGGTGTCT CTTACATCGT CCAGGAGCCT 
GGCGATTATG AAGTATCCAT CAAGTTCAAT GACGAGCACA TCCCTGACAG TCCGTTTGTG 
GTGCCTGTGG CCTCGCCATC TGACGATGCC CGCCGTCTCA CTGTTGCCAG TCTTCAGGAG 
TCCGGGTTAA AGGTGAACCA GCCGGCCTCT TTTGCTGTGA GCCTAAATGG GGCGAAAGGA 
GTCATCGATG CCAAAGTTCA CAGTCCATCA GGAGCACTTG AAGAGTGCTG CGTCACAGAG 
ATCGATGAAG ATAAATATGC TGTGAGATTC ATTCCCAGGG AGAATGGCCT GTACCTGATA 
GATGTGAAAT TTAATGGTTC TCACATCCCT GGAAGCCCCT TCAAGATCCG TGTTGGTGAG 
ACGGGTCAGG CTGGAGATCC AGGAATGGTG TCTGCTTATG GAGCTGGTCT GGAAGGAGGC 
ACCACTGGAT CGCCTTGTGA GTTCATTGTA AACACCAGTT CAGCTGGCCC TGGAGCTTTG 
GCGGTGACTA TCGACGGGCC GTCCAAGGTG AAGATGGACT GTCAGGAATG CCCAGAGGGC 
TACAAAGTCA CCTACACACC TATGGCCCCT GGAAACTACC TAATTTCTAT TAAATACGGT 
GGACCGTACC ATATTGTTGG TAGTCCATTC AAAGCTAAAA TTACTGGCTC CCGATTGGTC 
AACAGCCACA GCATGCATGA GACTTCTTCA GTACTGGTTG ACCCTGTGAC CCGCAGCATG 
ACGACCAGCC AGCAGGCAGC GCCAGGCTGG GCCAGCTCTG ATGCCAGCCG CGTAGTGGCC 
AAAGGTTTGG GGCTCAACAA GGGCTTCATA GGACAGAAGA ACACTTTCAG TGTGGACTGT 
AGTAAAGCAG GGAGGAACAT GCTCCTGGTC GGAGTGGATG GACCCAAAGT GCCCTGTGAG 
GAGATCCTGG TGAAACACCT GGGCAACCGC CTCTACAACG TTTCCTACCA GCTGAAGGAG 
AAAGGAGAGT ACATCCTGGT GGTCAAATGG GGTGATGAGC ACATCCCGGG CAGCCCGTAT 
CACATCACTG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

flna ( XM_021473624.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021329299.1 Danio rerio filamin A, alpha (actin binding protein 280) (flna), transcript variant X3, mRNA.

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CloneID	ODa146252
Clone ID Related Accession (Same CDS sequence)	XM_021473624.1
Accession Version	XM_021473624.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	filamin-A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395142.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCAAC ACACACGCCC CCACCAGTCG AGCGCCGCCG CTCCCCTATC CAACGCTTCT 
CTCTCCCCGG ACAAGGACGC CGACATGCCC GCTACAGAGA AAGATCTCGC CGAGGACGCG 
CCATGGAAGA AAATCCAGCA AAATACATTC ACGCGTTGGT GCAACGAGCA TCTGAAATGT 
GTCAACAAGC GCATAGCGAA CCTGCAGACG GACCTGAGCG ACGGGCTCCG GCTGATCGCG 
CTTCTGGAGG TGCTCAGCCA GAAGAAGATG TTCCGCAAGT ACAACCAGCG CCCTACCTTC 
AGACAGATGC AGCTGGAGAA CGTCTCCGTG GCGCTGGAGT TCCTGGACAT TGAAAACATC 
AAGCTGGTCT CCATTGACAG TAAGGCCATA GTGGATGGTA ACCTGAAGCT GATCCTGGGT 
CTGATATGGA CTCTTATTCT GCATTATTCC ATCTCCATGC CCATGTGGGA CGAGGAAGAG 
GAGAGCGAGG AAAGCAAGCA GAAGACGCCC AAGCAGAGGC TCCTCGGATG GATCCAGAAC 
AAGCTGCCCG AGCTGCCAAT CACCAACTTC AGCCGCGATT GGCAGTCAGG GAAAGCCCTT 
GGCGCTCTGG TGGACAGTTG TGCTCCAGGT CTGTGTCCAG ACTGGGATTC CTGGGACCAG 
ACCAAGCCTG TGGACAATGC CAGAGAGGCC ATGCAGCAGG CTGATGACTG GCTCGGTGTT 
CCTCAGGTCA TCACACCAGA AGAAATCGTT GATCCCAATG TGGACGAGCA CTCCGTTATG 
ACTTACCTGT CCCAGTTTCC CAAAGCCAAA CTCAAGCCTG GTGCCCCTCT GAGGCCCAAA 
CTCAATCCCA AAAAGGCCCG TGCTTATGGA CCAGGTATTG AGCCAACAGG TAATGTTGTG 
ATGAAGAAGG CTGTGTTCAC TGTTGAGACC ATCAGCGCTG GTCAGGGTGA GGTACTGGTT 
TATGTCGAGG ACCCCGCTGG ACACCGCGAG GAGGCCAAAG TCACAGCCAA CAATGACAAG 
AACCGCACCT ACTCAGTATT CTATGTGCCA AAAGTCACTG GACAACACAA GGTGACAGTG 
CTGTTTGCAG GGCAACACAT CTCTAAGAGC CCGTTTGAGG TGGATGTGGG AATGGCTCAG 
GGAGACTCCA GCAAGGTCAC TGCCCAGGGC CCAGGACTCG AGCCTGCAGG CAACATTGCC 
AACAAAACCA CATACTTTGA TGTCTACACA GCTGGTGCTG GAGTAGGAGA GGTGGAGGTG 
GTCATCATGG ACCCCAGTGG AAAGAAGGAC ACGGTTGAGT GCAGTGTCGA GGATAAAGGC 
AACAGCAGCT ACCGATGCAC CTACAAGCCC ACTCAGGAGG GACAGCACAT CATTTACATC 
ACCTTTGCCG GGGGACAGAT CTCCAAGAGC CCTTACACTG TCAATGTGGG AGAGGCCTGT 
AATCCAAGCC TGTGTACAGC CAAGGGCCGC GGTCTGCAGC CTAAAGGCCT GAGGGTCAAG 
GAGACCGCCG AGTTTAAGGT TTACACCAAA GGAGCAGGAA CCGGAGACCT CAAAGTTACC 
ATCAAAGGGC CCAAGGGTCT CGAGGAGCCC TGTAAGAAGA AGGATTTGGG AGACGGCGTG 
TACAGCTTTG ATTATTACCC CTCCACACCT GGAAATTACA TCATCACCAT CACATGGGGC 
GGTCAACACA TCCCACGCAG CCCGTTTGAG GTGAAGATCG GGTCTGAAGC AGGACCGCAG 
CAGGTCAGGG CGTGGGGTCC AGGACTAGAG AGTGGCGTTG TGGGCAAATC TGCTGACTTT 
GTTGTGGAAG CTGTTGGTGA TGATGTGGGC ACTTTAGGTT TCTCTGTTGA AGGCCCTTCC 
CCGGCTAAGA TTGAATGTGA TGATAAGGGA GATGGTTCAT GTGACGTGAG GTACTGGCCC 
ACCGAACCGG GTGAATATGC AGTTCATGTG CTCTGTAAGA ATGAGGACAT CCAGCTCAGC 
CCTTTCATGG CTGAAATCAT GCCCGCCCCA GGAAAAGACT TTTATCCTGA CAAGGTGAAG 
GCTTATGGCC CCGGTCTGCA GAGCTCAGGC CTGTCAGTTG GCAAACCAGC CGAGTTTACC 
GTGGATGCCA AACTGGGTGG CAAAGCTCCT CTGAAGATTC AAGCTCAGGA CCGTGATGGA 
AACCCAGTCG ATGTGCAGGT GAAGGACAAT GGAAATGGGA CATACAGCTG CAGCTACACC 
CCTCGCAAAC CAGTCAAACA CACTGTGATG GTGTCCTGGG GCGGCGTCAA CATCCCAGAG 
AGCCCGTTCA GAATGAACAT TGGGGCAGGG TGTCATCCCA ATAAGGTGAA AGTATCTGGA 
CCTGGAGTGG CCAAGACAGG CCTCAAAGCA TATGATCCAA CATACTTTAC TGTGGATTGT 
GCTGAGGCTG GACAGGGCGA CATCAGCATA GGCATCAAAT GTGCTGCAGG TGTGGTGGGA 
CCTGCTGAGG CTGACATAGA CTTCGACATC ATTAGAAATG ACAACGACAC ATTTACTGTT 
AAATACACTC CTCCTGGAGC GGGCAGCTAC ACCATCATGG TGCTGTTTGC TGATCAGACC 
ATTCCCATGA CACCCATCAG AATTAAGGTT GATCCCTCTC ATGATGCAAG CAAGGTTAAA 
GCTGAGGGGC CTGGACTTAG CCGTTCTGGT GTGGAGCTGA ACAAACCCAC TCACTTCACT 
GTGAACACAA AGGCTGCAGG CAAGGCCAAG CTTGATGCTC AGTTCACTGG ACCCAACAAG 
GGAGATGCAG TCCGTGACTT CGACATAATC GACAACCATG ATGGCACTCA CACTGTCAAA 
TATACCCCTG TCCAACAGGG CAACATGGGT TTGAATGTCA CCTACGGTGG TGACCCTATT 
CCCAAGAGCC CATTTGCAGT AGCAATTGCC CCCTCTCTGG ACCTCAGTAA AGTTAAAGTG 
GCTGGTCTTG GAAGTAAAAT GACTGTTGGA AAGGATCAGG AGATCACAGT CAAATCTAAA 
GGTGCAGGCG GTCAGGGCAA AGTTGGCGCA AAGGTTACTG GTCCATCTGC TAAATCAGTG 
CCCTGTAAAG TTGAACCAGG CCTGAGCCCA GAAACCAGCC AAGTTCGTTT TATCCCAAGA 
GATAAGGGAC CCTATGAGGT GGAACTGACA TATGATGGCG CCCCCATCCC TGGCAGTCCA 
TTCCCCATCG AAGCCATTGC ACCTACTGAC CCATCCAAGG TGCGATGCTC TGGGCCAGGA 
CTGGAGCGGG CGAAGGTTGG CGAGCCTGGA CAGTTTGTGG TGGATTGCAC TAATGCTGGC 
CCTGCTGAGT TGACCATCGA GATCATCTCT GACAATGGCA CTGAGGCTGA AGTCCACATT 
CAAGACAACG GCGACGGGAC CTACACCATC ACTTACGTCC CTCTCTACCC TGGAGCCTAT 
ACCCTCACCA TCCGCTACGG GGACCAGGAT GTGCCAAACT TCCCAGCAAG ACTCCATGTG 
GAGCCTGCTG TGGAGACCAG TGGAGTGAAA GTGTTCGGAC CCGGAGTGGA GGGCAAAGGT 
GTTTTCAGGG AAGCCACTAC AGATTTCACT GTGGATGCCC GTGCGCTCAC TAAAACCGGA 
GGCAATCACA TCAAAACCTG CATCAACAAC CCATCGGGCA ACCGCACTGA AGCACTGATC 
CGAGATCTGG GAGACGGCAC ATATCAAGTG GAGTACACGC CTTATGAGGA GGGTACACAC 
AGTGTTGAGG TTGCATATGA CAGCTCCCCG GTTCCCAAAA GCCCCTTCCG TGTGCCAGTG 
ACCGAGGGCT GTGACCCGGC GCGTGTGCGT GTGCATGGTC CAGGACTTCA GTCTGGCATC 
ACCAACAAAC CTAACAAATT TACCGTGGAG ACCCGTGGGG CTGGTACTGG TGGACTGGGG 
CTGGCTATGG AAGGACCTTC TGAGGCCAAA ATGTCCTGCA CCGATAACAA AGATGGCAGC 
TGTTGTGTTG AATACATCCC ATATGAGCCA GGGACATACA ACCTCAATGT CACCTATGGA 
GGCCAACCAA TCACTGGAAG CCCTTTCTCT GTACCTGTCC ATGATACTGT TGACCCAACT 
AAAGTGAAAT GTCAGGGCCA GGGGCTTGGA AACAACGTGC GTGCCAATAT CCCACAGGTC 
TTTTCTGTGG ATGCAAGCAA GGCTGGTATG GCGCCACTGC AGGTCAGAGT TCAGGGACCC 
AAAGGTGTTG TGGAGCCTGC TGAGGTGGTG GATAATGGTG ACCAGACTCA CACAGTCAGC 
TATGTGCCCA CAAGAGAGGG GCCATACTCC ATTAATGTTC TGTATGCTGA TGAGGAGATC 
CCACAGAGTC CTTATAAGGT GAAGGTTCTG CCCACTCATG ACGCAAGTAA AGTCCGTTGC 
AGCGGTCCTG GCCTGAATAC CACTGGTGTT CCTGCCAGTC TGCCCGTGGA GTTCACTATT 
GACGCCAAAG ATGCAGGAGA GGGACTGTTG GCCGTCCAGA TAACTGACCC TGATGGGAAG 
CCAAAGAAGG CTAACATTCG TGATAATCAG GACGGGACCT ACCTTGTGTC CTATGTGCCT 
GACATGACCG GCCGTTACAC CATTCTTATT AAGTATGGTG GTGATGAGAT CCCGTATTCT 
CCATATCGCA TTCGAGCCCT GCCTACTGGA GACGCCAGCA AGTGTACAGT CACAGTCTCA 
ATTGGAGGTC ATGGTTTGGG AGCTGGAGTT GGTCCGACTA TTCAGATTGG AGAGCAGACT 
GTCATCACTG TGGATGCGAA GGCAGCTGGG AAGGGCAAGG TGACCTGTAC CGTGTGCACA 
CCTGATGGAG GCGAGGTGGA TGTGGACGTC GTGGAAAATG AGGATGGAAC GTTTGACATC 
TTTTACACTG CACCGCAGCC TGGGAAATAC GTGATCTGTG TGCGCTTCGG TGGAGAGCAC 
ATCCCTAACA GCCCCTTCCA AGTCACGGCT ACAGATGGGC AATTGGGTAT GAATGGACTG 
GATGTAGCTG GACTGAGACC CTTTGACTTG GTCATTCCTT TCACCATTCA GAAAGGAGAA 
ATTACAGGTG ATGTACGAAT GCCCTCTGGA AAAGTGGCCA AGCCTGATAT TACAGACAAC 
AAGGATGGCA CAGTCACAGT GAAATATGCC CCAACTGAGG CGGGCCTGCA TGAGATGGAC 
ATTAAATATG ATGGAATACA CATTCCAGGA AGTCCATTGC AATTCTACGT GGATTATGTC 
AACAGCGGTC ATGTGACTGC ATATGGTCCT GGTCTAATTC ATGGCATGGT CAATAAGCCT 
GCAGTCTTCA CAGTCAACAC AAAGGATGCT GGAGAGGGTG GTCTGTCTCT GGCCATCGAG 
GGGCCATCAA AGGCAGATAT CAGCTGCACT GATAATCAGG ACGGTACCTG CACTGTTTCA 
TACCTCCCTG TTCTTCCCGG AGACTACAAT ATCATTGTTA AATACAATGA CAAGAACATC 
CCCGGCAGCC CTTTCATGTC CAAAATCACA GGTGACGACT CGATGCGCAT GTCTCACCTG 
AAGGTGGGCT CAGCTGCTGA CATCCCTCTG GACATTGGTG AGCTTGACCT CAGTCAGCTG 
ACAGCCACAC TCACCACCCC ATCCGGCCGA GAGGAGCCTT GCCTGCTCAA GATGCTCAGA 
AATGGGCATG TGGGAATTTC CTTTGTGCCC AAGGAAATTG GCGAGCATCT TGTTAACATT 
AAGAAAAATG GACGCCATAT TCCTAGTAGC CCCATTTCTG TCATGATCAA CCAATCAGAG 
ATCGGTGATG CCAGCCGTGT ACGAGTAACT GGTCAAGGCC TGAGTGAAGC TCGCACCTTT 
GAGCCCGCAG AATTCATCAT TGACACTAGA GATGCAGGAT ATGGTGGACT CAGTTTGTCT 
ATTGAGGGAC CTAGTAAAGT GGATATCAAC ACTGAAGATC AGGAAGATGG CACCTGCAAA 
GTGACATATT GCCCCACAGA GCCTGGAAAC TACATAATTA ACATCAAATT TGCAGACCAG 
CATGTGCCAG GAAGTGCTTT TACTGTCAAA GTGACAGGCG AGGGTCGGAT GAAGGAGAGC 
ATCACCCGCA AAAGAAGAGC TGCTTCTGTG GCCAATGTTG GCAGCCAGTG CGACCTCAGT 
CTAAAGATCC CTGAGATCAG CATTGCAGAC ATGACCGCTC AGGTGACCAG CCCATCTGGC 
AAAGTCCACA AGGCAGAGAT AATGGAGGGA GAAAACAACA CCTACTGCAT CCGATTCGTG 
CCCACAGAGA TGGGTGTGCA CACAGTAAGC GTGAAGTATC AAGGTCAGCA TGTGCCTGGA 
TCCCCCTTCC AGTTCACGGT GGGACCTCTT GGAGAAGGAG GAGCTCATAA AGTCAGAGCT 
GGAGGCCCAG GATTAGAGAG GGCAGAAGCT GGTGTACCAG CGGAGTTCAG CATTTGGACT 
CGTGAAGCAG GAGCTGGTGG TCTGTCCATC GCAGTGGAAG GACCCAGCAA AGCAGAGATT 
GCTTTTGAAG ACCGTAAAGA TGGATCCAGT GGTGTCTCTT ACATCGTCCA GGAGCCTGGC 
GATTATGAAG TATCCATCAA GTTCAATGAC GAGCACATCC CTGACAGTCC GTTTGTGGTG 
CCTGTGGCCT CGCCATCTGA CGATGCCCGC CGTCTCACTG TTGCCAGTCT TCAGGAGTCC 
GGGTTAAAGG TGAACCAGCC GGCCTCTTTT GCTGTGAGCC TAAATGGGGC GAAAGGAGTC 
ATCGATGCCA AAGTTCACAG TCCATCAGGA GCACTTGAAG AGTGCTGCGT CACAGAGATC 
GATGAAGATA AATATGCTGT GAGATTCATT CCCAGGGAGA ATGGCCTGTA CCTGATAGAT 
GTGAAATTTA ATGGTTCTCA CATCCCTGGA AGCCCCTTCA AGATCCGTGT TGGTGAGACG 
GGTCAGGCTG GAGATCCAGG AATGGTGTCT GCTTATGGAG CTGGTCTGGA AGGAGGCACC 
ACTGGATCGC CTTGTGAGTT CATTGTAAAC ACCAGTTCAG CTGGCCCTGG AGCTTTGGCG 
GTGACTATCG ACGGGCCGTC CAAGGTGAAG ATGGACTGTC AGGAATGCCC AGAGGGCTAC 
AAAGTCACCT ACACACCTAT GGCCCCTGGA AACTACCTAA TTTCTATTAA ATACGGTGGA 
CCGTACCATA TTGTTGGTAG TCCATTCAAA GCTAAAATTA CTGGCTCCCG ATTGGTCAAC 
AGCCACAGCA TGCATGAGAC TTCTTCAGTA CTGGTTGACC CTGTGACCCG CAGCATGACG 
ACCAGCCAGC AGGCAGCGCC AGGCTGGGCC AGCTCTGATG CCAGCCGCGT AGTGGCCAAA 
GGTTTGGGGC TCAACAAGGG CTTCATAGGA CAGAAGAACA CTTTCAGTGT GGACTGTAGT 
AAAGCAGGGA GGAACATGCT CCTGGTCGGA GTGGATGGAC CCAAAGTGCC CTGTGAGGAG 
ATCCTGGTGA AACACCTGGG CAACCGCCTC TACAACGTTT CCTACCAGCT GAAGGAGAAA 
GGAGAGTACA TCCTGGTGGT CAAATGGGGT GATGAGCACA TCCCGGGCAG CCCGTATCAC 
ATCACTGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021329299.1
CDS	353..7924
Translation	MSQHTRPHQSSAAAPLSNASLSPDKDADMPATEKDLAEDAPWKKIQQNTFTRWCNEHLKCVNKRIANLQTDLSDGLRLIALLEVLSQKKMFRKYNQRPTFRQMQLENVSVALEFLDIENIKLVSIDSKAIVDGNLKLILGLIWTLILHYSISMPMWDEEEESEESKQKTPKQRLLGWIQNKLPELPITNFSRDWQSGKALGALVDSCAPGLCPDWDSWDQTKPVDNAREAMQQADDWLGVPQVITPEEIVDPNVDEHSVMTYLSQFPKAKLKPGAPLRPKLNPKKARAYGPGIEPTGNVVMKKAVFTVETISAGQGEVLVYVEDPAGHREEAKVTANNDKNRTYSVFYVPKVTGQHKVTVLFAGQHISKSPFEVDVGMAQGDSSKVTAQGPGLEPAGNIANKTTYFDVYTAGAGVGEVEVVIMDPSGKKDTVECSVEDKGNSSYRCTYKPTQEGQHIIYITFAGGQISKSPYTVNVGEACNPSLCTAKGRGLQPKGLRVKETAEFKVYTKGAGTGDLKVTIKGPKGLEEPCKKKDLGDGVYSFDYYPSTPGNYIITITWGGQHIPRSPFEVKIGSEAGPQQVRAWGPGLESGVVGKSADFVVEAVGDDVGTLGFSVEGPSPAKIECDDKGDGSCDVRYWPTEPGEYAVHVLCKNEDIQLSPFMAEIMPAPGKDFYPDKVKAYGPGLQSSGLSVGKPAEFTVDAKLGGKAPLKIQAQDRDGNPVDVQVKDNGNGTYSCSYTPRKPVKHTVMVSWGGVNIPESPFRMNIGAGCHPNKVKVSGPGVAKTGLKAYDPTYFTVDCAEAGQGDISIGIKCAAGVVGPAEADIDFDIIRNDNDTFTVKYTPPGAGSYTIMVLFADQTIPMTPIRIKVDPSHDASKVKAEGPGLSRSGVELNKPTHFTVNTKAAGKAKLDAQFTGPNKGDAVRDFDIIDNHDGTHTVKYTPVQQGNMGLNVTYGGDPIPKSPFAVAIAPSLDLSKVKVAGLGSKMTVGKDQEITVKSKGAGGQGKVGAKVTGPSAKSVPCKVEPGLSPETSQVRFIPRDKGPYEVELTYDGAPIPGSPFPIEAIAPTDPSKVRCSGPGLERAKVGEPGQFVVDCTNAGPAELTIEIISDNGTEAEVHIQDNGDGTYTITYVPLYPGAYTLTIRYGDQDVPNFPARLHVEPAVETSGVKVFGPGVEGKGVFREATTDFTVDARALTKTGGNHIKTCINNPSGNRTEALIRDLGDGTYQVEYTPYEEGTHSVEVAYDSSPVPKSPFRVPVTEGCDPARVRVHGPGLQSGITNKPNKFTVETRGAGTGGLGLAMEGPSEAKMSCTDNKDGSCCVEYIPYEPGTYNLNVTYGGQPITGSPFSVPVHDTVDPTKVKCQGQGLGNNVRANIPQVFSVDASKAGMAPLQVRVQGPKGVVEPAEVVDNGDQTHTVSYVPTREGPYSINVLYADEEIPQSPYKVKVLPTHDASKVRCSGPGLNTTGVPASLPVEFTIDAKDAGEGLLAVQITDPDGKPKKANIRDNQDGTYLVSYVPDMTGRYTILIKYGGDEIPYSPYRIRALPTGDASKCTVTVSIGGHGLGAGVGPTIQIGEQTVITVDAKAAGKGKVTCTVCTPDGGEVDVDVVENEDGTFDIFYTAPQPGKYVICVRFGGEHIPNSPFQVTATDGQLGMNGLDVAGLRPFDLVIPFTIQKGEITGDVRMPSGKVAKPDITDNKDGTVTVKYAPTEAGLHEMDIKYDGIHIPGSPLQFYVDYVNSGHVTAYGPGLIHGMVNKPAVFTVNTKDAGEGGLSLAIEGPSKADISCTDNQDGTCTVSYLPVLPGDYNIIVKYNDKNIPGSPFMSKITGDDSMRMSHLKVGSAADIPLDIGELDLSQLTATLTTPSGREEPCLLKMLRNGHVGISFVPKEIGEHLVNIKKNGRHIPSSPISVMINQSEIGDASRVRVTGQGLSEARTFEPAEFIIDTRDAGYGGLSLSIEGPSKVDINTEDQEDGTCKVTYCPTEPGNYIINIKFADQHVPGSAFTVKVTGEGRMKESITRKRRAASVANVGSQCDLSLKIPEISIADMTAQVTSPSGKVHKAEIMEGENNTYCIRFVPTEMGVHTVSVKYQGQHVPGSPFQFTVGPLGEGGAHKVRAGGPGLERAEAGVPAEFSIWTREAGAGGLSIAVEGPSKAEIAFEDRKDGSSGVSYIVQEPGDYEVSIKFNDEHIPDSPFVVPVASPSDDARRLTVASLQESGLKVNQPASFAVSLNGAKGVIDAKVHSPSGALEECCVTEIDEDKYAVRFIPRENGLYLIDVKFNGSHIPGSPFKIRVGETGQAGDPGMVSAYGAGLEGGTTGSPCEFIVNTSSAGPGALAVTIDGPSKVKMDCQECPEGYKVTYTPMAPGNYLISIKYGGPYHIVGSPFKAKITGSRLVNSHSMHETSSVLVDPVTRSMTTSQQAAPGWASSDASRVVAKGLGLNKGFIGQKNTFSVDCSKAGRNMLLVGVDGPKVPCEEILVKHLGNRLYNVSYQLKEKGEYILVVKWGDEHIPGSPYHITV

Target ORF information:

RefSeq Version	XM_021473624.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio filamin A, alpha (actin binding protein 280) (flna), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473624.1

ORF Insert Sequence:

ATGAGTCAAC ACACACGCCC CCACCAGTCG AGCGCCGCCG CTCCCCTATC CAACGCTTCT 
CTCTCCCCGG ACAAGGACGC CGACATGCCC GCTACAGAGA AAGATCTCGC CGAGGACGCG 
CCATGGAAGA AAATCCAGCA AAATACATTC ACGCGTTGGT GCAACGAGCA TCTGAAATGT 
GTCAACAAGC GCATAGCGAA CCTGCAGACG GACCTGAGCG ACGGGCTCCG GCTGATCGCG 
CTTCTGGAGG TGCTCAGCCA GAAGAAGATG TTCCGCAAGT ACAACCAGCG CCCTACCTTC 
AGACAGATGC AGCTGGAGAA CGTCTCCGTG GCGCTGGAGT TCCTGGACAT TGAAAACATC 
AAGCTGGTCT CCATTGACAG TAAGGCCATA GTGGATGGTA ACCTGAAGCT GATCCTGGGT 
CTGATATGGA CTCTTATTCT GCATTATTCC ATCTCCATGC CCATGTGGGA CGAGGAAGAG 
GAGAGCGAGG AAAGCAAGCA GAAGACGCCC AAGCAGAGGC TCCTCGGATG GATCCAGAAC 
AAGCTGCCCG AGCTGCCAAT CACCAACTTC AGCCGCGATT GGCAGTCAGG GAAAGCCCTT 
GGCGCTCTGG TGGACAGTTG TGCTCCAGGT CTGTGTCCAG ACTGGGATTC CTGGGACCAG 
ACCAAGCCTG TGGACAATGC CAGAGAGGCC ATGCAGCAGG CTGATGACTG GCTCGGTGTT 
CCTCAGGTCA TCACACCAGA AGAAATCGTT GATCCCAATG TGGACGAGCA CTCCGTTATG 
ACTTACCTGT CCCAGTTTCC CAAAGCCAAA CTCAAGCCTG GTGCCCCTCT GAGGCCCAAA 
CTCAATCCCA AAAAGGCCCG TGCTTATGGA CCAGGTATTG AGCCAACAGG TAATGTTGTG 
ATGAAGAAGG CTGTGTTCAC TGTTGAGACC ATCAGCGCTG GTCAGGGTGA GGTACTGGTT 
TATGTCGAGG ACCCCGCTGG ACACCGCGAG GAGGCCAAAG TCACAGCCAA CAATGACAAG 
AACCGCACCT ACTCAGTATT CTATGTGCCA AAAGTCACTG GACAACACAA GGTGACAGTG 
CTGTTTGCAG GGCAACACAT CTCTAAGAGC CCGTTTGAGG TGGATGTGGG AATGGCTCAG 
GGAGACTCCA GCAAGGTCAC TGCCCAGGGC CCAGGACTCG AGCCTGCAGG CAACATTGCC 
AACAAAACCA CATACTTTGA TGTCTACACA GCTGGTGCTG GAGTAGGAGA GGTGGAGGTG 
GTCATCATGG ACCCCAGTGG AAAGAAGGAC ACGGTTGAGT GCAGTGTCGA GGATAAAGGC 
AACAGCAGCT ACCGATGCAC CTACAAGCCC ACTCAGGAGG GACAGCACAT CATTTACATC 
ACCTTTGCCG GGGGACAGAT CTCCAAGAGC CCTTACACTG TCAATGTGGG AGAGGCCTGT 
AATCCAAGCC TGTGTACAGC CAAGGGCCGC GGTCTGCAGC CTAAAGGCCT GAGGGTCAAG 
GAGACCGCCG AGTTTAAGGT TTACACCAAA GGAGCAGGAA CCGGAGACCT CAAAGTTACC 
ATCAAAGGGC CCAAGGGTCT CGAGGAGCCC TGTAAGAAGA AGGATTTGGG AGACGGCGTG 
TACAGCTTTG ATTATTACCC CTCCACACCT GGAAATTACA TCATCACCAT CACATGGGGC 
GGTCAACACA TCCCACGCAG CCCGTTTGAG GTGAAGATCG GGTCTGAAGC AGGACCGCAG 
CAGGTCAGGG CGTGGGGTCC AGGACTAGAG AGTGGCGTTG TGGGCAAATC TGCTGACTTT 
GTTGTGGAAG CTGTTGGTGA TGATGTGGGC ACTTTAGGTT TCTCTGTTGA AGGCCCTTCC 
CCGGCTAAGA TTGAATGTGA TGATAAGGGA GATGGTTCAT GTGACGTGAG GTACTGGCCC 
ACCGAACCGG GTGAATATGC AGTTCATGTG CTCTGTAAGA ATGAGGACAT CCAGCTCAGC 
CCTTTCATGG CTGAAATCAT GCCCGCCCCA GGAAAAGACT TTTATCCTGA CAAGGTGAAG 
GCTTATGGCC CCGGTCTGCA GAGCTCAGGC CTGTCAGTTG GCAAACCAGC CGAGTTTACC 
GTGGATGCCA AACTGGGTGG CAAAGCTCCT CTGAAGATTC AAGCTCAGGA CCGTGATGGA 
AACCCAGTCG ATGTGCAGGT GAAGGACAAT GGAAATGGGA CATACAGCTG CAGCTACACC 
CCTCGCAAAC CAGTCAAACA CACTGTGATG GTGTCCTGGG GCGGCGTCAA CATCCCAGAG 
AGCCCGTTCA GAATGAACAT TGGGGCAGGG TGTCATCCCA ATAAGGTGAA AGTATCTGGA 
CCTGGAGTGG CCAAGACAGG CCTCAAAGCA TATGATCCAA CATACTTTAC TGTGGATTGT 
GCTGAGGCTG GACAGGGCGA CATCAGCATA GGCATCAAAT GTGCTGCAGG TGTGGTGGGA 
CCTGCTGAGG CTGACATAGA CTTCGACATC ATTAGAAATG ACAACGACAC ATTTACTGTT 
AAATACACTC CTCCTGGAGC GGGCAGCTAC ACCATCATGG TGCTGTTTGC TGATCAGACC 
ATTCCCATGA CACCCATCAG AATTAAGGTT GATCCCTCTC ATGATGCAAG CAAGGTTAAA 
GCTGAGGGGC CTGGACTTAG CCGTTCTGGT GTGGAGCTGA ACAAACCCAC TCACTTCACT 
GTGAACACAA AGGCTGCAGG CAAGGCCAAG CTTGATGCTC AGTTCACTGG ACCCAACAAG 
GGAGATGCAG TCCGTGACTT CGACATAATC GACAACCATG ATGGCACTCA CACTGTCAAA 
TATACCCCTG TCCAACAGGG CAACATGGGT TTGAATGTCA CCTACGGTGG TGACCCTATT 
CCCAAGAGCC CATTTGCAGT AGCAATTGCC CCCTCTCTGG ACCTCAGTAA AGTTAAAGTG 
GCTGGTCTTG GAAGTAAAAT GACTGTTGGA AAGGATCAGG AGATCACAGT CAAATCTAAA 
GGTGCAGGCG GTCAGGGCAA AGTTGGCGCA AAGGTTACTG GTCCATCTGC TAAATCAGTG 
CCCTGTAAAG TTGAACCAGG CCTGAGCCCA GAAACCAGCC AAGTTCGTTT TATCCCAAGA 
GATAAGGGAC CCTATGAGGT GGAACTGACA TATGATGGCG CCCCCATCCC TGGCAGTCCA 
TTCCCCATCG AAGCCATTGC ACCTACTGAC CCATCCAAGG TGCGATGCTC TGGGCCAGGA 
CTGGAGCGGG CGAAGGTTGG CGAGCCTGGA CAGTTTGTGG TGGATTGCAC TAATGCTGGC 
CCTGCTGAGT TGACCATCGA GATCATCTCT GACAATGGCA CTGAGGCTGA AGTCCACATT 
CAAGACAACG GCGACGGGAC CTACACCATC ACTTACGTCC CTCTCTACCC TGGAGCCTAT 
ACCCTCACCA TCCGCTACGG GGACCAGGAT GTGCCAAACT TCCCAGCAAG ACTCCATGTG 
GAGCCTGCTG TGGAGACCAG TGGAGTGAAA GTGTTCGGAC CCGGAGTGGA GGGCAAAGGT 
GTTTTCAGGG AAGCCACTAC AGATTTCACT GTGGATGCCC GTGCGCTCAC TAAAACCGGA 
GGCAATCACA TCAAAACCTG CATCAACAAC CCATCGGGCA ACCGCACTGA AGCACTGATC 
CGAGATCTGG GAGACGGCAC ATATCAAGTG GAGTACACGC CTTATGAGGA GGGTACACAC 
AGTGTTGAGG TTGCATATGA CAGCTCCCCG GTTCCCAAAA GCCCCTTCCG TGTGCCAGTG 
ACCGAGGGCT GTGACCCGGC GCGTGTGCGT GTGCATGGTC CAGGACTTCA GTCTGGCATC 
ACCAACAAAC CTAACAAATT TACCGTGGAG ACCCGTGGGG CTGGTACTGG TGGACTGGGG 
CTGGCTATGG AAGGACCTTC TGAGGCCAAA ATGTCCTGCA CCGATAACAA AGATGGCAGC 
TGTTGTGTTG AATACATCCC ATATGAGCCA GGGACATACA ACCTCAATGT CACCTATGGA 
GGCCAACCAA TCACTGGAAG CCCTTTCTCT GTACCTGTCC ATGATACTGT TGACCCAACT 
AAAGTGAAAT GTCAGGGCCA GGGGCTTGGA AACAACGTGC GTGCCAATAT CCCACAGGTC 
TTTTCTGTGG ATGCAAGCAA GGCTGGTATG GCGCCACTGC AGGTCAGAGT TCAGGGACCC 
AAAGGTGTTG TGGAGCCTGC TGAGGTGGTG GATAATGGTG ACCAGACTCA CACAGTCAGC 
TATGTGCCCA CAAGAGAGGG GCCATACTCC ATTAATGTTC TGTATGCTGA TGAGGAGATC 
CCACAGAGTC CTTATAAGGT GAAGGTTCTG CCCACTCATG ACGCAAGTAA AGTCCGTTGC 
AGCGGTCCTG GCCTGAATAC CACTGGTGTT CCTGCCAGTC TGCCCGTGGA GTTCACTATT 
GACGCCAAAG ATGCAGGAGA GGGACTGTTG GCCGTCCAGA TAACTGACCC TGATGGGAAG 
CCAAAGAAGG CTAACATTCG TGATAATCAG GACGGGACCT ACCTTGTGTC CTATGTGCCT 
GACATGACCG GCCGTTACAC CATTCTTATT AAGTATGGTG GTGATGAGAT CCCGTATTCT 
CCATATCGCA TTCGAGCCCT GCCTACTGGA GACGCCAGCA AGTGTACAGT CACAGTCTCA 
ATTGGAGGTC ATGGTTTGGG AGCTGGAGTT GGTCCGACTA TTCAGATTGG AGAGCAGACT 
GTCATCACTG TGGATGCGAA GGCAGCTGGG AAGGGCAAGG TGACCTGTAC CGTGTGCACA 
CCTGATGGAG GCGAGGTGGA TGTGGACGTC GTGGAAAATG AGGATGGAAC GTTTGACATC 
TTTTACACTG CACCGCAGCC TGGGAAATAC GTGATCTGTG TGCGCTTCGG TGGAGAGCAC 
ATCCCTAACA GCCCCTTCCA AGTCACGGCT ACAGATGGGC AATTGGGTAT GAATGGACTG 
GATGTAGCTG GACTGAGACC CTTTGACTTG GTCATTCCTT TCACCATTCA GAAAGGAGAA 
ATTACAGGTG ATGTACGAAT GCCCTCTGGA AAAGTGGCCA AGCCTGATAT TACAGACAAC 
AAGGATGGCA CAGTCACAGT GAAATATGCC CCAACTGAGG CGGGCCTGCA TGAGATGGAC 
ATTAAATATG ATGGAATACA CATTCCAGGA AGTCCATTGC AATTCTACGT GGATTATGTC 
AACAGCGGTC ATGTGACTGC ATATGGTCCT GGTCTAATTC ATGGCATGGT CAATAAGCCT 
GCAGTCTTCA CAGTCAACAC AAAGGATGCT GGAGAGGGTG GTCTGTCTCT GGCCATCGAG 
GGGCCATCAA AGGCAGATAT CAGCTGCACT GATAATCAGG ACGGTACCTG CACTGTTTCA 
TACCTCCCTG TTCTTCCCGG AGACTACAAT ATCATTGTTA AATACAATGA CAAGAACATC 
CCCGGCAGCC CTTTCATGTC CAAAATCACA GGTGACGACT CGATGCGCAT GTCTCACCTG 
AAGGTGGGCT CAGCTGCTGA CATCCCTCTG GACATTGGTG AGCTTGACCT CAGTCAGCTG 
ACAGCCACAC TCACCACCCC ATCCGGCCGA GAGGAGCCTT GCCTGCTCAA GATGCTCAGA 
AATGGGCATG TGGGAATTTC CTTTGTGCCC AAGGAAATTG GCGAGCATCT TGTTAACATT 
AAGAAAAATG GACGCCATAT TCCTAGTAGC CCCATTTCTG TCATGATCAA CCAATCAGAG 
ATCGGTGATG CCAGCCGTGT ACGAGTAACT GGTCAAGGCC TGAGTGAAGC TCGCACCTTT 
GAGCCCGCAG AATTCATCAT TGACACTAGA GATGCAGGAT ATGGTGGACT CAGTTTGTCT 
ATTGAGGGAC CTAGTAAAGT GGATATCAAC ACTGAAGATC AGGAAGATGG CACCTGCAAA 
GTGACATATT GCCCCACAGA GCCTGGAAAC TACATAATTA ACATCAAATT TGCAGACCAG 
CATGTGCCAG GAAGTGCTTT TACTGTCAAA GTGACAGGCG AGGGTCGGAT GAAGGAGAGC 
ATCACCCGCA AAAGAAGAGC TGCTTCTGTG GCCAATGTTG GCAGCCAGTG CGACCTCAGT 
CTAAAGATCC CTGAGATCAG CATTGCAGAC ATGACCGCTC AGGTGACCAG CCCATCTGGC 
AAAGTCCACA AGGCAGAGAT AATGGAGGGA GAAAACAACA CCTACTGCAT CCGATTCGTG 
CCCACAGAGA TGGGTGTGCA CACAGTAAGC GTGAAGTATC AAGGTCAGCA TGTGCCTGGA 
TCCCCCTTCC AGTTCACGGT GGGACCTCTT GGAGAAGGAG GAGCTCATAA AGTCAGAGCT 
GGAGGCCCAG GATTAGAGAG GGCAGAAGCT GGTGTACCAG CGGAGTTCAG CATTTGGACT 
CGTGAAGCAG GAGCTGGTGG TCTGTCCATC GCAGTGGAAG GACCCAGCAA AGCAGAGATT 
GCTTTTGAAG ACCGTAAAGA TGGATCCAGT GGTGTCTCTT ACATCGTCCA GGAGCCTGGC 
GATTATGAAG TATCCATCAA GTTCAATGAC GAGCACATCC CTGACAGTCC GTTTGTGGTG 
CCTGTGGCCT CGCCATCTGA CGATGCCCGC CGTCTCACTG TTGCCAGTCT TCAGGAGTCC 
GGGTTAAAGG TGAACCAGCC GGCCTCTTTT GCTGTGAGCC TAAATGGGGC GAAAGGAGTC 
ATCGATGCCA AAGTTCACAG TCCATCAGGA GCACTTGAAG AGTGCTGCGT CACAGAGATC 
GATGAAGATA AATATGCTGT GAGATTCATT CCCAGGGAGA ATGGCCTGTA CCTGATAGAT 
GTGAAATTTA ATGGTTCTCA CATCCCTGGA AGCCCCTTCA AGATCCGTGT TGGTGAGACG 
GGTCAGGCTG GAGATCCAGG AATGGTGTCT GCTTATGGAG CTGGTCTGGA AGGAGGCACC 
ACTGGATCGC CTTGTGAGTT CATTGTAAAC ACCAGTTCAG CTGGCCCTGG AGCTTTGGCG 
GTGACTATCG ACGGGCCGTC CAAGGTGAAG ATGGACTGTC AGGAATGCCC AGAGGGCTAC 
AAAGTCACCT ACACACCTAT GGCCCCTGGA AACTACCTAA TTTCTATTAA ATACGGTGGA 
CCGTACCATA TTGTTGGTAG TCCATTCAAA GCTAAAATTA CTGGCTCCCG ATTGGTCAAC 
AGCCACAGCA TGCATGAGAC TTCTTCAGTA CTGGTTGACC CTGTGACCCG CAGCATGACG 
ACCAGCCAGC AGGCAGCGCC AGGCTGGGCC AGCTCTGATG CCAGCCGCGT AGTGGCCAAA 
GGTTTGGGGC TCAACAAGGG CTTCATAGGA CAGAAGAACA CTTTCAGTGT GGACTGTAGT 
AAAGCAGGGA GGAACATGCT CCTGGTCGGA GTGGATGGAC CCAAAGTGCC CTGTGAGGAG 
ATCCTGGTGA AACACCTGGG CAACCGCCTC TACAACGTTT CCTACCAGCT GAAGGAGAAA 
GGAGAGTACA TCCTGGTGGT CAAATGGGGT GATGAGCACA TCCCGGGCAG CCCGTATCAC 
ATCACTGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

flna ( XM_021474525.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330200.1 Danio rerio filamin A, alpha (actin binding protein 280) (flna), transcript variant X3, mRNA.

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CloneID	ODa57477
Clone ID Related Accession (Same CDS sequence)	XM_021474525.1 , XM_017353690.2 , XM_017353690.1
Accession Version	XM_021474525.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	filamin-A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395361.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCAAC ACACACGCCC CCACCAGTCG AGCGCCGCCG CTCCCCTATC CAACGCTTCT 
CTCTCCCCGG ACAAGGACGC CGACATGCCC GCTACAGAGA AAGATCTCGC CGAGGACGCG 
CCATGGAAGA AAATCCAGCA AAATACATTC ACGCGTTGGT GCAACGAGCA TCTGAAATGT 
GTCAACAAGC GCATAGCGAA CCTGCAGACG GACCTGAGCG ACGGGCTCCG GCTGATCGCG 
CTTCTGGAGG TGCTCAGCCA GAAGAAGATG TTCCGCAAGT ACAACCAGCG CCCTACCTTC 
AGACAGATGC AGCTGGAGAA CGTCTCCGTG GCGCTGGAGT TCCTGGACAT TGAAAACATC 
AAGCTGGTCT CCATTGACAG TAAGGCCATA GTGGATGGTA ACCTGAAGCT GATCCTGGGT 
CTGATATGGA CTCTTATTCT GCATTATTCC ATCTCCATGC CCATGTGGGA CGAGGAAGAG 
GAGAGCGAGG AAAGCAAGCA GAAGACGCCC AAGCAGAGGC TCCTCGGATG GATCCAGAAC 
AAGCTGCCCG AGCTGCCAAT CACCAACTTC AGCCGCGATT GGCAGTCAGG GAAAGCCCTT 
GGCGCTCTGG TGGACAGTTG TGCTCCAGGT CTGTGTCCAG ACTGGGATTC CTGGGACCAG 
ACCAAGCCTG TGGACAATGC CAGAGAGGCC ATGCAGCAGG CTGATGACTG GCTCGGTGTT 
CCTCAGGTCA TCACACCAGA AGAAATCGTT GATCCCAATG TGGACGAGCA CTCCGTTATG 
ACTTACCTGT CCCAGTTTCC CAAAGCCAAA CTCAAGCCTG GTGCCCCTCT GAGGCCCAAA 
CTCAATCCCA AAAAGGCCCG TGCTTATGGA CCAGGTATTG AGCCAACAGG TAATGTTGTG 
ATGAAGAAGG CTGTGTTCAC TGTTGAGACC ATCAGCGCTG GTCAGGGTGA GGTACTGGTT 
TATGTCGAGG ACCCCGCTGG ACACCGCGAG GAGGCCAAAG TCACAGCCAA CAATGACAAG 
AACCGCACCT ACTCAGTATT CTATGTGCCA AAAGTCACTG GACAACACAA GGTGACAGTG 
CTGTTTGCAG GGCAACACAT CTCTAAGAGC CCGTTTGAGG TGGATGTGGG AATGGCTCAG 
GGAGACTCCA GCAAGGTCAC TGCCCAGGGC CCAGGACTCG AGCCTGCAGG CAACATTGCC 
AACAAAACCA CATACTTTGA TGTCTACACA GCTGGTGCTG GAGTAGGAGA GGTGGAGGTG 
GTCATCATGG ACCCCAGTGG AAAGAAGGAC ACGGTTGAGT GCAGTGTCGA GGATAAAGGC 
AACAGCAGCT ACCGATGCAC CTACAAGCCC ACTCAGGAGG GACAGCACAT CATTTACATC 
ACCTTTGCCG GGGGACAGAT CTCCAAGAGC CCTTACACTG TCAATGTGGG AGAGGCCTGT 
AATCCAAGCC TGTGTACAGC CAAGGGCCGC GGTCTGCAGC CTAAAGGCCT GAGGGTCAAG 
GAGACCGCCG AGTTTAAGGT TTACACCAAA GGAGCAGGAA CCGGAGACCT CAAAGTTACC 
ATCAAAGGGC CCAAGGGTCT CGAGGAGCCC TGTAAGAAGA AGGATTTGGG AGACGGCGTG 
TACAGCTTTG ATTATTACCC CTCCACACCT GGAAATTACA TCATCACCAT CACATGGGGC 
GGTCAACACA TCCCACGCAG CCCGTTTGAG GTGAAGATCG GGTCTGAAGC AGGACCGCAG 
CAGGTCAGGG CGTGGGGTCC AGGACTAGAG AGTGGCGTTG TGGGCAAATC TGCTGACTTT 
GTTGTGGAAG CTGTTGGTGA TGATGTGGGC ACTTTAGGTT TCTCTGTTGA AGGCCCTTCC 
CCGGCTAAGA TTGAATGTGA TGATAAGGGA GATGGTTCAT GTGACGTGAG GTACTGGCCC 
ACCGAACCGG GTGAATATGC AGTTCATGTG CTCTGTAAGA ATGAGGACAT CCAGCTCAGC 
CCTTTCATGG CTGAAATCAT GCCCGCCCCA GGAAAAGACT TTTATCCTGA CAAGGTGAAG 
GCTTATGGCC CCGGTCTGCA GAGCTCAGGC CTGTCAGTTG GCAAACCAGC CGAGTTTACC 
GTGGATGCCA AACTGGGTGG CAAAGCTCCT CTGAAGATTC AAGCTCAGGA CCGTGATGGA 
AACCCAGTCG ATGTGCAGGT GAAGGACAAT GGAAATGGGA CATACAGCTG CAGCTACACC 
CCTCGCAAAC CAGTCAAACA CACTGTGATG GTGTCCTGGG GCGGCGTCAA CATCCCAGAG 
AGCCCGTTCA GAATGAACAT TGGGGCAGGG TGTCATCCCA ATAAGGTGAA AGTATCTGGA 
CCTGGAGTGG CCAAGACAGG CCTCAAAGCA TATGATCCAA CATACTTTAC TGTGGATTGT 
GCTGAGGCTG GACAGGGCGA CATCAGCATA GGCATCAAAT GTGCTGCAGG TGTGGTGGGA 
CCTGCTGAGG CTGACATAGA CTTCGACATC ATTAGAAATG ACAACGACAC ATTTACTGTT 
AAATACACTC CTCCTGGAGC GGGCAGCTAC ACCATCATGG TGCTGTTTGC TGATCAGACC 
ATTCCCATGA CACCCATCAG AATTAAGGTT GATCCCTCTC ATGATGCAAG CAAGGTTAAA 
GCTGAGGGGC CTGGACTTAG CCGTTCTGGT GTGGAGCTGA ACAAACCCAC TCACTTCACT 
GTGAACACAA AGGCTGCAGG CAAGGCCAAG CTTGATGCTC AGTTCACTGG ACCCAACAAG 
GGAGATGCAG TCCGTGACTT CGACATAATC GACAACCATG ATGGCACTCA CACTGTCAAA 
TATACCCCTG TCCAACAGGG CAACATGGGT TTGAATGTCA CCTACGGTGG TGACCCTATT 
CCCAAGAGCC CATTTGCAGT AGCAATTGCC CCCTCTCTGG ACCTCAGTAA AGTTAAAGTG 
GCTGGTCTTG GAAGTAAAAT GACTGTTGGA AAGGATCAGG AGATCACAGT CAAATCTAAA 
GGTGCAGGCG GTCAGGGCAA AGTTGGCGCA AAGGTTACTG GTCCATCTGC TAAATCAGTG 
CCCTGTAAAG TTGAACCAGG CCTGAGCCCA GAAACCAGCC AAGTTCGTTT TATCCCAAGA 
GATAAGGGAC CCTATGAGGT GGAACTGACA TATGATGGCG CCCCCATCCC TGGCAGTCCA 
TTCCCCATCG AAGCCATTGC ACCTACTGAC CCATCCAAGG TGCGATGCTC TGGGCCAGGA 
CTGGAGCGGG CGAAGGTTGG CGAGCCTGGA CAGTTTGTGG TGGATTGCAC TAATGCTGGC 
CCTGCTGAGT TGACCATCGA GATCATCTCT GACAATGGCA CTGAGGCTGA AGTCCACATT 
CAAGACAACG GCGACGGGAC CTACACCATC ACTTACGTCC CTCTCTACCC TGGAGCCTAT 
ACCCTCACCA TCCGCTACGG GGACCAGGAT GTGCCAAACT TCCCAGCAAG ACTCCATGTG 
GAGCCTGCTG TGGAGACCAG TGGAGTGAAA GTGTTCGGAC CCGGAGTGGA GGGCAAAGGT 
GTTTTCAGGG AAGCCACTAC AGATTTCACT GTGGATGCCC GTGCGCTCAC TAAAACCGGA 
GGCAATCACA TCAAAACCTG CATCAACAAC CCATCGGGCA ACCGCACTGA AGCACTGATC 
CGAGATCTGG GAGACGGCAC ATATCAAGTG GAGTACACGC CTTATGAGGA GGGTACACAC 
AGTGTTGAGG TTGCATATGA CAGCTCCCCG GTTCCCAAAA GCCCCTTCCG TGTGCCAGTG 
ACCGAGGGCT GTGACCCGGC GCGTGTGCGT GTGCATGGTC CAGGACTTCA GTCTGGCATC 
ACCAACAAAC CTAACAAATT TACCGTGGAG ACCCGTGGGG CTGGTACTGG TGGACTGGGG 
CTGGCTATGG AAGGACCTTC TGAGGCCAAA ATGTCCTGCA CCGATAACAA AGATGGCAGC 
TGTTGTGTTG AATACATCCC ATATGAGCCA GGGACATACA ACCTCAATGT CACCTATGGA 
GGCCAACCAA TCACTGGAAG CCCTTTCTCT GTACCTGTCC ATGATACTGT TGACCCAACT 
AAAGTGAAAT GTCAGGGCCA GGGGCTTGGA AACAACGTGC GTGCCAATAT CCCACAGGTC 
TTTTCTGTGG ATGCAAGCAA GGCTGGTATG GCGCCACTGC AGGTCAGAGT TCAGGGACCC 
AAAGGTGTTG TGGAGCCTGC TGAGGTGGTG GATAATGGTG ACCAGACTCA CACAGTCAGC 
TATGTGCCCA CAAGAGAGGG GCCATACTCC ATTAATGTTC TGTATGCTGA TGAGGAGATC 
CCACAGAGTC CTTATAAGGT GAAGGTTCTG CCCACTCATG ACGCAAGTAA AGTCCGTTGC 
AGCGGTCCTG GCCTGAATAC CACTGGTGTT CCTGCCAGTC TGCCCGTGGA GTTCACTATT 
GACGCCAAAG ATGCAGGAGA GGGACTGTTG GCCGTCCAGA TAACTGACCC TGATGGGAAG 
CCAAAGAAGG CTAACATTCG TGATAATCAG GACGGGACCT ACCTTGTGTC CTATGTGCCT 
GACATGACCG GCCGTTACAC CATTCTTATT AAGTATGGTG GTGATGAGAT CCCGTATTCT 
CCATATCGCA TTCGAGCCCT GCCTACTGGA GACGCCAGCA AGTGTACAGT CACAGTCTCA 
ATTGGAGGTC ATGGTTTGGG AGCTGGAGTT GGTCCGACTA TTCAGATTGG AGAGCAGACT 
GTCATCACTG TGGATGCGAA GGCAGCTGGG AAGGGCAAGG TGACCTGTAC CGTGTGCACA 
CCTGATGGAG GCGAGGTGGA TGTGGACGTC GTGGAAAATG AGGATGGAAC GTTTGACATC 
TTTTACACTG CACCGCAGCC TGGGAAATAC GTGATCTGTG TGCGCTTCGG TGGAGAGCAC 
ATCCCTAACA GCCCCTTCCA AGTCACGGCT ACAGATGGGC AATTGGGTAT GAATGGACTG 
GATGTAGCTG GACTGAGACC CTTTGACTTG GTCATTCCTT TCACCATTCA GAAAGGAGAA 
ATTACAGGTG ATGTACGAAT GCCCTCTGGA AAAGTGGCCA AGCCTGATAT TACAGACAAC 
AAGGATGGCA CAGTCACAGT GAAATATGCC CCAACTGAGG CGGGCCTGCA TGAGATGGAC 
ATTAAATATG ATGGAATACA CATTCCAGGA AGTCCATTGC AATTCTACGT GGATTATGTC 
AACAGCGGTC ATGTGACTGC ATATGGTCCT GGTCTAATTC ATGGCATGGT CAATAAGCCT 
GCAGTCTTCA CAGTCAACAC AAAGGATGCT GGAGAGGGTG GTCTGTCTCT GGCCATCGAG 
GGGCCATCAA AGGCAGATAT CAGCTGCACT GATAATCAGG ACGGTACCTG CACTGTTTCA 
TACCTCCCTG TTCTTCCCGG AGACTACAAT ATCATTGTTA AATACAATGA CAAGAACATC 
CCCGGCAGCC CTTTCATGTC CAAAATCACA GGTGACGACT CGATGCGCAT GTCTCACCTG 
AAGGTGGGCT CAGCTGCTGA CATCCCTCTG GACATTGGTG AGCTTGACCT CAGTCAGCTG 
ACAGCCACAC TCACCACCCC ATCCGGCCGA GAGGAGCCTT GCCTGCTCAA GATGCTCAGA 
AATGGGCATG TGGGAATTTC CTTTGTGCCC AAGGAAATTG GCGAGCATCT TGTTAACATT 
AAGAAAAATG GACGCCATAT TCCTAGTAGC CCCATTTCTG TCATGATCAA CCAATCAGAG 
ATCGGTGATG CCAGCCGTGT ACGAGTAACT GGTCAAGGCC TGAGTGAAGC TCGCACCTTT 
GAGCCCGCAG AATTCATCAT TGACACTAGA GATGCAGGAT ATGGTGGACT CAGTTTGTCT 
ATTGAGGGAC CTAGTAAAGT GGATATCAAC ACTGAAGATC AGGAAGATGG CACCTGCAAA 
GTGACATATT GCCCCACAGA GCCTGGAAAC TACATAATTA ACATCAAATT TGCAGACCAG 
CATGTGCCAG GAAGTGCTTT CACTGTGAAA GTGACAGGCG AGGGTCGGAT GAAGGAGAGC 
ATCACCCGCA AAAGAAGAGC TGCTTCTGTG GCCAATGTTG GCAGCCAGTG CGACCTCAGT 
CTAAAGATCC CTGAGATCAG CATTGCAGAC ATGACCGCTC AGGTGACCAG CCCATCTGGC 
AAAGTCCACA AGGCAGAGAT AATGGAGGGA GAAAACAACA CCTACTGCAT CCGATTCGTG 
CCCACAGAGA TGGGTGTGCA CACAGTAAGC GTGAAGTATC AAGGTCAGCA TGTGCCTGGA 
TCCCCCTTCC AGTTCACGGT GGGACCTCTT GGAGAAGGAG GAGCTCATAA AGTCAGAGCT 
GGAGGCCCAG GATTAGAGAG GGCAGAAGCT GGTGTACCAG CGGAGTTCAG CATTTGGACT 
CGTGAAGCAG GAGCTGGTGG TCTGTCCATC GCAGTGGAAG GACCCAGCAA AGCAGAGATT 
GCTTTTGAAG ACCGTAAAGA TGGATCCAGT GGTGTCTCTT ACATCGTCCA GGAGCCTGGC 
GATTATGAAG TATCCATCAA GTTCAATGAC GAGCACATCC CTGACAGTCC GTTTGTGGTG 
CCTGTGGCCT CGCCATCTGA CGATGCCCGC CGTCTCACTG TTGCCAGTCT TCAGGAGTCC 
GGGTTAAAGG TGAACCAGCC GGCCTCTTTT GCTGTGAGCC TAAATGGGGC GAAAGGAGTC 
ATCGATGCCA AAGTTCACAG TCCATCAGGA GCACTTGAAG AGTGCTGCGT CACCGAGATC 
GATGAAGATA AATATGCTGT GAGATTCATT CCCAGGGAGA ATGGATTGTA CCTGATAGAT 
GTGAAATTTA ATGGTTCTCA CATCCCTGGA AGCCCCTTCA AGATCCGTGT TGGTGAGACG 
GGTCAGGCTG GAGATCCAGG AATGGTGTCT GCTTATGGAG CTGGTCTGGA AGGAGGCACT 
ACTGGATCGC CTTGTGAGTT CATTGTAAAC ACCAGTTCAG CTGGCCCTGG AGCTTTGGCG 
GTGACTATCG ACGGGCCGTC CAAGGTGAAG ATGGACTGTC AGGAATGCCC AGAGGGCTAC 
AAAGTCACCT ACACACCTAT GGCCCCTGGA AACTACCTAA TTTCTATTAA ATACGGTGGA 
CCGTACCATA TTGTTGGTAG TCCATTCAAA GCTAAAATTA CTGGCTCCCG ATTGGTCAAC 
AGCCACAGCA TGCATGAGAC TTCATCAGTA CTGGTTGACC CTGTGACCCG CAGCATGACG 
ACCAGCCAGC AGGCAGCGCC AGGCTGGGCC AGCTCTGATG CCAGCCGCGT AGTGGCCAAA 
GGTTTGGGGC TCAACAAGGG CTTCATAGGA CAGAAGAACA CTTTCAGTGT GGACTGTAGT 
AAAGCAGGGA GGAACATGCT CCTGGTCGGA GTGGATGGAC CCAAAGTGCC CTGTGAGGAG 
ATCCTGGTGA AACACCTGGG CAACCGCCTC TACAACGTTT CCTACCAGCT GAAGGAGAAA 
GGAGAGTACA TTCTGGTGGT CAAATGGGGT GATGAGCACA TCCCGGGCAG CCCGTATCAC 
ATCACTGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330200.1
CDS	356..7927
Translation	MSQHTRPHQSSAAAPLSNASLSPDKDADMPATEKDLAEDAPWKKIQQNTFTRWCNEHLKCVNKRIANLQTDLSDGLRLIALLEVLSQKKMFRKYNQRPTFRQMQLENVSVALEFLDIENIKLVSIDSKAIVDGNLKLILGLIWTLILHYSISMPMWDEEEESEESKQKTPKQRLLGWIQNKLPELPITNFSRDWQSGKALGALVDSCAPGLCPDWDSWDQTKPVDNAREAMQQADDWLGVPQVITPEEIVDPNVDEHSVMTYLSQFPKAKLKPGAPLRPKLNPKKARAYGPGIEPTGNVVMKKAVFTVETISAGQGEVLVYVEDPAGHREEAKVTANNDKNRTYSVFYVPKVTGQHKVTVLFAGQHISKSPFEVDVGMAQGDSSKVTAQGPGLEPAGNIANKTTYFDVYTAGAGVGEVEVVIMDPSGKKDTVECSVEDKGNSSYRCTYKPTQEGQHIIYITFAGGQISKSPYTVNVGEACNPSLCTAKGRGLQPKGLRVKETAEFKVYTKGAGTGDLKVTIKGPKGLEEPCKKKDLGDGVYSFDYYPSTPGNYIITITWGGQHIPRSPFEVKIGSEAGPQQVRAWGPGLESGVVGKSADFVVEAVGDDVGTLGFSVEGPSPAKIECDDKGDGSCDVRYWPTEPGEYAVHVLCKNEDIQLSPFMAEIMPAPGKDFYPDKVKAYGPGLQSSGLSVGKPAEFTVDAKLGGKAPLKIQAQDRDGNPVDVQVKDNGNGTYSCSYTPRKPVKHTVMVSWGGVNIPESPFRMNIGAGCHPNKVKVSGPGVAKTGLKAYDPTYFTVDCAEAGQGDISIGIKCAAGVVGPAEADIDFDIIRNDNDTFTVKYTPPGAGSYTIMVLFADQTIPMTPIRIKVDPSHDASKVKAEGPGLSRSGVELNKPTHFTVNTKAAGKAKLDAQFTGPNKGDAVRDFDIIDNHDGTHTVKYTPVQQGNMGLNVTYGGDPIPKSPFAVAIAPSLDLSKVKVAGLGSKMTVGKDQEITVKSKGAGGQGKVGAKVTGPSAKSVPCKVEPGLSPETSQVRFIPRDKGPYEVELTYDGAPIPGSPFPIEAIAPTDPSKVRCSGPGLERAKVGEPGQFVVDCTNAGPAELTIEIISDNGTEAEVHIQDNGDGTYTITYVPLYPGAYTLTIRYGDQDVPNFPARLHVEPAVETSGVKVFGPGVEGKGVFREATTDFTVDARALTKTGGNHIKTCINNPSGNRTEALIRDLGDGTYQVEYTPYEEGTHSVEVAYDSSPVPKSPFRVPVTEGCDPARVRVHGPGLQSGITNKPNKFTVETRGAGTGGLGLAMEGPSEAKMSCTDNKDGSCCVEYIPYEPGTYNLNVTYGGQPITGSPFSVPVHDTVDPTKVKCQGQGLGNNVRANIPQVFSVDASKAGMAPLQVRVQGPKGVVEPAEVVDNGDQTHTVSYVPTREGPYSINVLYADEEIPQSPYKVKVLPTHDASKVRCSGPGLNTTGVPASLPVEFTIDAKDAGEGLLAVQITDPDGKPKKANIRDNQDGTYLVSYVPDMTGRYTILIKYGGDEIPYSPYRIRALPTGDASKCTVTVSIGGHGLGAGVGPTIQIGEQTVITVDAKAAGKGKVTCTVCTPDGGEVDVDVVENEDGTFDIFYTAPQPGKYVICVRFGGEHIPNSPFQVTATDGQLGMNGLDVAGLRPFDLVIPFTIQKGEITGDVRMPSGKVAKPDITDNKDGTVTVKYAPTEAGLHEMDIKYDGIHIPGSPLQFYVDYVNSGHVTAYGPGLIHGMVNKPAVFTVNTKDAGEGGLSLAIEGPSKADISCTDNQDGTCTVSYLPVLPGDYNIIVKYNDKNIPGSPFMSKITGDDSMRMSHLKVGSAADIPLDIGELDLSQLTATLTTPSGREEPCLLKMLRNGHVGISFVPKEIGEHLVNIKKNGRHIPSSPISVMINQSEIGDASRVRVTGQGLSEARTFEPAEFIIDTRDAGYGGLSLSIEGPSKVDINTEDQEDGTCKVTYCPTEPGNYIINIKFADQHVPGSAFTVKVTGEGRMKESITRKRRAASVANVGSQCDLSLKIPEISIADMTAQVTSPSGKVHKAEIMEGENNTYCIRFVPTEMGVHTVSVKYQGQHVPGSPFQFTVGPLGEGGAHKVRAGGPGLERAEAGVPAEFSIWTREAGAGGLSIAVEGPSKAEIAFEDRKDGSSGVSYIVQEPGDYEVSIKFNDEHIPDSPFVVPVASPSDDARRLTVASLQESGLKVNQPASFAVSLNGAKGVIDAKVHSPSGALEECCVTEIDEDKYAVRFIPRENGLYLIDVKFNGSHIPGSPFKIRVGETGQAGDPGMVSAYGAGLEGGTTGSPCEFIVNTSSAGPGALAVTIDGPSKVKMDCQECPEGYKVTYTPMAPGNYLISIKYGGPYHIVGSPFKAKITGSRLVNSHSMHETSSVLVDPVTRSMTTSQQAAPGWASSDASRVVAKGLGLNKGFIGQKNTFSVDCSKAGRNMLLVGVDGPKVPCEEILVKHLGNRLYNVSYQLKEKGEYILVVKWGDEHIPGSPYHITV

Target ORF information:

RefSeq Version	XM_021474525.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio filamin A, alpha (actin binding protein 280) (flna), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474525.1

ORF Insert Sequence:

ATGAGTCAAC ACACACGCCC CCACCAGTCG AGCGCCGCCG CTCCCCTATC CAACGCTTCT 
CTCTCCCCGG ACAAGGACGC CGACATGCCC GCTACAGAGA AAGATCTCGC CGAGGACGCG 
CCATGGAAGA AAATCCAGCA AAATACATTC ACGCGTTGGT GCAACGAGCA TCTGAAATGT 
GTCAACAAGC GCATAGCGAA CCTGCAGACG GACCTGAGCG ACGGGCTCCG GCTGATCGCG 
CTTCTGGAGG TGCTCAGCCA GAAGAAGATG TTCCGCAAGT ACAACCAGCG CCCTACCTTC 
AGACAGATGC AGCTGGAGAA CGTCTCCGTG GCGCTGGAGT TCCTGGACAT TGAAAACATC 
AAGCTGGTCT CCATTGACAG TAAGGCCATA GTGGATGGTA ACCTGAAGCT GATCCTGGGT 
CTGATATGGA CTCTTATTCT GCATTATTCC ATCTCCATGC CCATGTGGGA CGAGGAAGAG 
GAGAGCGAGG AAAGCAAGCA GAAGACGCCC AAGCAGAGGC TCCTCGGATG GATCCAGAAC 
AAGCTGCCCG AGCTGCCAAT CACCAACTTC AGCCGCGATT GGCAGTCAGG GAAAGCCCTT 
GGCGCTCTGG TGGACAGTTG TGCTCCAGGT CTGTGTCCAG ACTGGGATTC CTGGGACCAG 
ACCAAGCCTG TGGACAATGC CAGAGAGGCC ATGCAGCAGG CTGATGACTG GCTCGGTGTT 
CCTCAGGTCA TCACACCAGA AGAAATCGTT GATCCCAATG TGGACGAGCA CTCCGTTATG 
ACTTACCTGT CCCAGTTTCC CAAAGCCAAA CTCAAGCCTG GTGCCCCTCT GAGGCCCAAA 
CTCAATCCCA AAAAGGCCCG TGCTTATGGA CCAGGTATTG AGCCAACAGG TAATGTTGTG 
ATGAAGAAGG CTGTGTTCAC TGTTGAGACC ATCAGCGCTG GTCAGGGTGA GGTACTGGTT 
TATGTCGAGG ACCCCGCTGG ACACCGCGAG GAGGCCAAAG TCACAGCCAA CAATGACAAG 
AACCGCACCT ACTCAGTATT CTATGTGCCA AAAGTCACTG GACAACACAA GGTGACAGTG 
CTGTTTGCAG GGCAACACAT CTCTAAGAGC CCGTTTGAGG TGGATGTGGG AATGGCTCAG 
GGAGACTCCA GCAAGGTCAC TGCCCAGGGC CCAGGACTCG AGCCTGCAGG CAACATTGCC 
AACAAAACCA CATACTTTGA TGTCTACACA GCTGGTGCTG GAGTAGGAGA GGTGGAGGTG 
GTCATCATGG ACCCCAGTGG AAAGAAGGAC ACGGTTGAGT GCAGTGTCGA GGATAAAGGC 
AACAGCAGCT ACCGATGCAC CTACAAGCCC ACTCAGGAGG GACAGCACAT CATTTACATC 
ACCTTTGCCG GGGGACAGAT CTCCAAGAGC CCTTACACTG TCAATGTGGG AGAGGCCTGT 
AATCCAAGCC TGTGTACAGC CAAGGGCCGC GGTCTGCAGC CTAAAGGCCT GAGGGTCAAG 
GAGACCGCCG AGTTTAAGGT TTACACCAAA GGAGCAGGAA CCGGAGACCT CAAAGTTACC 
ATCAAAGGGC CCAAGGGTCT CGAGGAGCCC TGTAAGAAGA AGGATTTGGG AGACGGCGTG 
TACAGCTTTG ATTATTACCC CTCCACACCT GGAAATTACA TCATCACCAT CACATGGGGC 
GGTCAACACA TCCCACGCAG CCCGTTTGAG GTGAAGATCG GGTCTGAAGC AGGACCGCAG 
CAGGTCAGGG CGTGGGGTCC AGGACTAGAG AGTGGCGTTG TGGGCAAATC TGCTGACTTT 
GTTGTGGAAG CTGTTGGTGA TGATGTGGGC ACTTTAGGTT TCTCTGTTGA AGGCCCTTCC 
CCGGCTAAGA TTGAATGTGA TGATAAGGGA GATGGTTCAT GTGACGTGAG GTACTGGCCC 
ACCGAACCGG GTGAATATGC AGTTCATGTG CTCTGTAAGA ATGAGGACAT CCAGCTCAGC 
CCTTTCATGG CTGAAATCAT GCCCGCCCCA GGAAAAGACT TTTATCCTGA CAAGGTGAAG 
GCTTATGGCC CCGGTCTGCA GAGCTCAGGC CTGTCAGTTG GCAAACCAGC CGAGTTTACC 
GTGGATGCCA AACTGGGTGG CAAAGCTCCT CTGAAGATTC AAGCTCAGGA CCGTGATGGA 
AACCCAGTCG ATGTGCAGGT GAAGGACAAT GGAAATGGGA CATACAGCTG CAGCTACACC 
CCTCGCAAAC CAGTCAAACA CACTGTGATG GTGTCCTGGG GCGGCGTCAA CATCCCAGAG 
AGCCCGTTCA GAATGAACAT TGGGGCAGGG TGTCATCCCA ATAAGGTGAA AGTATCTGGA 
CCTGGAGTGG CCAAGACAGG CCTCAAAGCA TATGATCCAA CATACTTTAC TGTGGATTGT 
GCTGAGGCTG GACAGGGCGA CATCAGCATA GGCATCAAAT GTGCTGCAGG TGTGGTGGGA 
CCTGCTGAGG CTGACATAGA CTTCGACATC ATTAGAAATG ACAACGACAC ATTTACTGTT 
AAATACACTC CTCCTGGAGC GGGCAGCTAC ACCATCATGG TGCTGTTTGC TGATCAGACC 
ATTCCCATGA CACCCATCAG AATTAAGGTT GATCCCTCTC ATGATGCAAG CAAGGTTAAA 
GCTGAGGGGC CTGGACTTAG CCGTTCTGGT GTGGAGCTGA ACAAACCCAC TCACTTCACT 
GTGAACACAA AGGCTGCAGG CAAGGCCAAG CTTGATGCTC AGTTCACTGG ACCCAACAAG 
GGAGATGCAG TCCGTGACTT CGACATAATC GACAACCATG ATGGCACTCA CACTGTCAAA 
TATACCCCTG TCCAACAGGG CAACATGGGT TTGAATGTCA CCTACGGTGG TGACCCTATT 
CCCAAGAGCC CATTTGCAGT AGCAATTGCC CCCTCTCTGG ACCTCAGTAA AGTTAAAGTG 
GCTGGTCTTG GAAGTAAAAT GACTGTTGGA AAGGATCAGG AGATCACAGT CAAATCTAAA 
GGTGCAGGCG GTCAGGGCAA AGTTGGCGCA AAGGTTACTG GTCCATCTGC TAAATCAGTG 
CCCTGTAAAG TTGAACCAGG CCTGAGCCCA GAAACCAGCC AAGTTCGTTT TATCCCAAGA 
GATAAGGGAC CCTATGAGGT GGAACTGACA TATGATGGCG CCCCCATCCC TGGCAGTCCA 
TTCCCCATCG AAGCCATTGC ACCTACTGAC CCATCCAAGG TGCGATGCTC TGGGCCAGGA 
CTGGAGCGGG CGAAGGTTGG CGAGCCTGGA CAGTTTGTGG TGGATTGCAC TAATGCTGGC 
CCTGCTGAGT TGACCATCGA GATCATCTCT GACAATGGCA CTGAGGCTGA AGTCCACATT 
CAAGACAACG GCGACGGGAC CTACACCATC ACTTACGTCC CTCTCTACCC TGGAGCCTAT 
ACCCTCACCA TCCGCTACGG GGACCAGGAT GTGCCAAACT TCCCAGCAAG ACTCCATGTG 
GAGCCTGCTG TGGAGACCAG TGGAGTGAAA GTGTTCGGAC CCGGAGTGGA GGGCAAAGGT 
GTTTTCAGGG AAGCCACTAC AGATTTCACT GTGGATGCCC GTGCGCTCAC TAAAACCGGA 
GGCAATCACA TCAAAACCTG CATCAACAAC CCATCGGGCA ACCGCACTGA AGCACTGATC 
CGAGATCTGG GAGACGGCAC ATATCAAGTG GAGTACACGC CTTATGAGGA GGGTACACAC 
AGTGTTGAGG TTGCATATGA CAGCTCCCCG GTTCCCAAAA GCCCCTTCCG TGTGCCAGTG 
ACCGAGGGCT GTGACCCGGC GCGTGTGCGT GTGCATGGTC CAGGACTTCA GTCTGGCATC 
ACCAACAAAC CTAACAAATT TACCGTGGAG ACCCGTGGGG CTGGTACTGG TGGACTGGGG 
CTGGCTATGG AAGGACCTTC TGAGGCCAAA ATGTCCTGCA CCGATAACAA AGATGGCAGC 
TGTTGTGTTG AATACATCCC ATATGAGCCA GGGACATACA ACCTCAATGT CACCTATGGA 
GGCCAACCAA TCACTGGAAG CCCTTTCTCT GTACCTGTCC ATGATACTGT TGACCCAACT 
AAAGTGAAAT GTCAGGGCCA GGGGCTTGGA AACAACGTGC GTGCCAATAT CCCACAGGTC 
TTTTCTGTGG ATGCAAGCAA GGCTGGTATG GCGCCACTGC AGGTCAGAGT TCAGGGACCC 
AAAGGTGTTG TGGAGCCTGC TGAGGTGGTG GATAATGGTG ACCAGACTCA CACAGTCAGC 
TATGTGCCCA CAAGAGAGGG GCCATACTCC ATTAATGTTC TGTATGCTGA TGAGGAGATC 
CCACAGAGTC CTTATAAGGT GAAGGTTCTG CCCACTCATG ACGCAAGTAA AGTCCGTTGC 
AGCGGTCCTG GCCTGAATAC CACTGGTGTT CCTGCCAGTC TGCCCGTGGA GTTCACTATT 
GACGCCAAAG ATGCAGGAGA GGGACTGTTG GCCGTCCAGA TAACTGACCC TGATGGGAAG 
CCAAAGAAGG CTAACATTCG TGATAATCAG GACGGGACCT ACCTTGTGTC CTATGTGCCT 
GACATGACCG GCCGTTACAC CATTCTTATT AAGTATGGTG GTGATGAGAT CCCGTATTCT 
CCATATCGCA TTCGAGCCCT GCCTACTGGA GACGCCAGCA AGTGTACAGT CACAGTCTCA 
ATTGGAGGTC ATGGTTTGGG AGCTGGAGTT GGTCCGACTA TTCAGATTGG AGAGCAGACT 
GTCATCACTG TGGATGCGAA GGCAGCTGGG AAGGGCAAGG TGACCTGTAC CGTGTGCACA 
CCTGATGGAG GCGAGGTGGA TGTGGACGTC GTGGAAAATG AGGATGGAAC GTTTGACATC 
TTTTACACTG CACCGCAGCC TGGGAAATAC GTGATCTGTG TGCGCTTCGG TGGAGAGCAC 
ATCCCTAACA GCCCCTTCCA AGTCACGGCT ACAGATGGGC AATTGGGTAT GAATGGACTG 
GATGTAGCTG GACTGAGACC CTTTGACTTG GTCATTCCTT TCACCATTCA GAAAGGAGAA 
ATTACAGGTG ATGTACGAAT GCCCTCTGGA AAAGTGGCCA AGCCTGATAT TACAGACAAC 
AAGGATGGCA CAGTCACAGT GAAATATGCC CCAACTGAGG CGGGCCTGCA TGAGATGGAC 
ATTAAATATG ATGGAATACA CATTCCAGGA AGTCCATTGC AATTCTACGT GGATTATGTC 
AACAGCGGTC ATGTGACTGC ATATGGTCCT GGTCTAATTC ATGGCATGGT CAATAAGCCT 
GCAGTCTTCA CAGTCAACAC AAAGGATGCT GGAGAGGGTG GTCTGTCTCT GGCCATCGAG 
GGGCCATCAA AGGCAGATAT CAGCTGCACT GATAATCAGG ACGGTACCTG CACTGTTTCA 
TACCTCCCTG TTCTTCCCGG AGACTACAAT ATCATTGTTA AATACAATGA CAAGAACATC 
CCCGGCAGCC CTTTCATGTC CAAAATCACA GGTGACGACT CGATGCGCAT GTCTCACCTG 
AAGGTGGGCT CAGCTGCTGA CATCCCTCTG GACATTGGTG AGCTTGACCT CAGTCAGCTG 
ACAGCCACAC TCACCACCCC ATCCGGCCGA GAGGAGCCTT GCCTGCTCAA GATGCTCAGA 
AATGGGCATG TGGGAATTTC CTTTGTGCCC AAGGAAATTG GCGAGCATCT TGTTAACATT 
AAGAAAAATG GACGCCATAT TCCTAGTAGC CCCATTTCTG TCATGATCAA CCAATCAGAG 
ATCGGTGATG CCAGCCGTGT ACGAGTAACT GGTCAAGGCC TGAGTGAAGC TCGCACCTTT 
GAGCCCGCAG AATTCATCAT TGACACTAGA GATGCAGGAT ATGGTGGACT CAGTTTGTCT 
ATTGAGGGAC CTAGTAAAGT GGATATCAAC ACTGAAGATC AGGAAGATGG CACCTGCAAA 
GTGACATATT GCCCCACAGA GCCTGGAAAC TACATAATTA ACATCAAATT TGCAGACCAG 
CATGTGCCAG GAAGTGCTTT CACTGTGAAA GTGACAGGCG AGGGTCGGAT GAAGGAGAGC 
ATCACCCGCA AAAGAAGAGC TGCTTCTGTG GCCAATGTTG GCAGCCAGTG CGACCTCAGT 
CTAAAGATCC CTGAGATCAG CATTGCAGAC ATGACCGCTC AGGTGACCAG CCCATCTGGC 
AAAGTCCACA AGGCAGAGAT AATGGAGGGA GAAAACAACA CCTACTGCAT CCGATTCGTG 
CCCACAGAGA TGGGTGTGCA CACAGTAAGC GTGAAGTATC AAGGTCAGCA TGTGCCTGGA 
TCCCCCTTCC AGTTCACGGT GGGACCTCTT GGAGAAGGAG GAGCTCATAA AGTCAGAGCT 
GGAGGCCCAG GATTAGAGAG GGCAGAAGCT GGTGTACCAG CGGAGTTCAG CATTTGGACT 
CGTGAAGCAG GAGCTGGTGG TCTGTCCATC GCAGTGGAAG GACCCAGCAA AGCAGAGATT 
GCTTTTGAAG ACCGTAAAGA TGGATCCAGT GGTGTCTCTT ACATCGTCCA GGAGCCTGGC 
GATTATGAAG TATCCATCAA GTTCAATGAC GAGCACATCC CTGACAGTCC GTTTGTGGTG 
CCTGTGGCCT CGCCATCTGA CGATGCCCGC CGTCTCACTG TTGCCAGTCT TCAGGAGTCC 
GGGTTAAAGG TGAACCAGCC GGCCTCTTTT GCTGTGAGCC TAAATGGGGC GAAAGGAGTC 
ATCGATGCCA AAGTTCACAG TCCATCAGGA GCACTTGAAG AGTGCTGCGT CACCGAGATC 
GATGAAGATA AATATGCTGT GAGATTCATT CCCAGGGAGA ATGGATTGTA CCTGATAGAT 
GTGAAATTTA ATGGTTCTCA CATCCCTGGA AGCCCCTTCA AGATCCGTGT TGGTGAGACG 
GGTCAGGCTG GAGATCCAGG AATGGTGTCT GCTTATGGAG CTGGTCTGGA AGGAGGCACT 
ACTGGATCGC CTTGTGAGTT CATTGTAAAC ACCAGTTCAG CTGGCCCTGG AGCTTTGGCG 
GTGACTATCG ACGGGCCGTC CAAGGTGAAG ATGGACTGTC AGGAATGCCC AGAGGGCTAC 
AAAGTCACCT ACACACCTAT GGCCCCTGGA AACTACCTAA TTTCTATTAA ATACGGTGGA 
CCGTACCATA TTGTTGGTAG TCCATTCAAA GCTAAAATTA CTGGCTCCCG ATTGGTCAAC 
AGCCACAGCA TGCATGAGAC TTCATCAGTA CTGGTTGACC CTGTGACCCG CAGCATGACG 
ACCAGCCAGC AGGCAGCGCC AGGCTGGGCC AGCTCTGATG CCAGCCGCGT AGTGGCCAAA 
GGTTTGGGGC TCAACAAGGG CTTCATAGGA CAGAAGAACA CTTTCAGTGT GGACTGTAGT 
AAAGCAGGGA GGAACATGCT CCTGGTCGGA GTGGATGGAC CCAAAGTGCC CTGTGAGGAG 
ATCCTGGTGA AACACCTGGG CAACCGCCTC TACAACGTTT CCTACCAGCT GAAGGAGAAA 
GGAGAGTACA TTCTGGTGGT CAAATGGGGT GATGAGCACA TCCCGGGCAG CCCGTATCAC 
ATCACTGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

flna ( XM_017353690.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209179.1 Danio rerio filamin A, alpha (actin binding protein 280) (flna), transcript variant X3, mRNA.

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CloneID	ODa57477
Clone ID Related Accession (Same CDS sequence)	XM_021474525.1 , XM_017353690.2 , XM_017353690.1
Accession Version	XM_017353690.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	filamin-A isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017353690.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCAAC ACACACGCCC CCACCAGTCG AGCGCCGCCG CTCCCCTATC CAACGCTTCT 
CTCTCCCCGG ACAAGGACGC CGACATGCCC GCTACAGAGA AAGATCTCGC CGAGGACGCG 
CCATGGAAGA AAATCCAGCA AAATACATTC ACGCGTTGGT GCAACGAGCA TCTGAAATGT 
GTCAACAAGC GCATAGCGAA CCTGCAGACG GACCTGAGCG ACGGGCTCCG GCTGATCGCG 
CTTCTGGAGG TGCTCAGCCA GAAGAAGATG TTCCGCAAGT ACAACCAGCG CCCTACCTTC 
AGACAGATGC AGCTGGAGAA CGTCTCCGTG GCGCTGGAGT TCCTGGACAT TGAAAACATC 
AAGCTGGTCT CCATTGACAG TAAGGCCATA GTGGATGGTA ACCTGAAGCT GATCCTGGGT 
CTGATATGGA CTCTTATTCT GCATTATTCC ATCTCCATGC CCATGTGGGA CGAGGAAGAG 
GAGAGCGAGG AAAGCAAGCA GAAGACGCCC AAGCAGAGGC TCCTCGGATG GATCCAGAAC 
AAGCTGCCCG AGCTGCCAAT CACCAACTTC AGCCGCGATT GGCAGTCAGG GAAAGCCCTT 
GGCGCTCTGG TGGACAGTTG TGCTCCAGGT CTGTGTCCAG ACTGGGATTC CTGGGACCAG 
ACCAAGCCTG TGGACAATGC CAGAGAGGCC ATGCAGCAGG CTGATGACTG GCTCGGTGTT 
CCTCAGGTCA TCACACCAGA AGAAATCGTT GATCCCAATG TGGACGAGCA CTCCGTTATG 
ACTTACCTGT CCCAGTTTCC CAAAGCCAAA CTCAAGCCTG GTGCCCCTCT GAGGCCCAAA 
CTCAATCCCA AAAAGGCCCG TGCTTATGGA CCAGGTATTG AGCCAACAGG TAATGTTGTG 
ATGAAGAAGG CTGTGTTCAC TGTTGAGACC ATCAGCGCTG GTCAGGGTGA GGTACTGGTT 
TATGTCGAGG ACCCCGCTGG ACACCGCGAG GAGGCCAAAG TCACAGCCAA CAATGACAAG 
AACCGCACCT ACTCAGTATT CTATGTGCCA AAAGTCACTG GACAACACAA GGTGACAGTG 
CTGTTTGCAG GGCAACACAT CTCTAAGAGC CCGTTTGAGG TGGATGTGGG AATGGCTCAG 
GGAGACTCCA GCAAGGTCAC TGCCCAGGGC CCAGGACTCG AGCCTGCAGG CAACATTGCC 
AACAAAACCA CATACTTTGA TGTCTACACA GCTGGTGCTG GAGTAGGAGA GGTGGAGGTG 
GTCATCATGG ACCCCAGTGG AAAGAAGGAC ACGGTTGAGT GCAGTGTCGA GGATAAAGGC 
AACAGCAGCT ACCGATGCAC CTACAAGCCC ACTCAGGAGG GACAGCACAT CATTTACATC 
ACCTTTGCCG GGGGACAGAT CTCCAAGAGC CCTTACACTG TCAATGTGGG AGAGGCCTGT 
AATCCAAGCC TGTGTACAGC CAAGGGCCGC GGTCTGCAGC CTAAAGGCCT GAGGGTCAAG 
GAGACCGCCG AGTTTAAGGT TTACACCAAA GGAGCAGGAA CCGGAGACCT CAAAGTTACC 
ATCAAAGGGC CCAAGGGTCT CGAGGAGCCC TGTAAGAAGA AGGATTTGGG AGACGGCGTG 
TACAGCTTTG ATTATTACCC CTCCACACCT GGAAATTACA TCATCACCAT CACATGGGGC 
GGTCAACACA TCCCACGCAG CCCGTTTGAG GTGAAGATCG GGTCTGAAGC AGGACCGCAG 
CAGGTCAGGG CGTGGGGTCC AGGACTAGAG AGTGGCGTTG TGGGCAAATC TGCTGACTTT 
GTTGTGGAAG CTGTTGGTGA TGATGTGGGC ACTTTAGGTT TCTCTGTTGA AGGCCCTTCC 
CCGGCTAAGA TTGAATGTGA TGATAAGGGA GATGGTTCAT GTGACGTGAG GTACTGGCCC 
ACCGAACCGG GTGAATATGC AGTTCATGTG CTCTGTAAGA ATGAGGACAT CCAGCTCAGC 
CCTTTCATGG CTGAAATCAT GCCCGCCCCA GGAAAAGACT TTTATCCTGA CAAGGTGAAG 
GCTTATGGCC CCGGTCTGCA GAGCTCAGGC CTGTCAGTTG GCAAACCAGC CGAGTTTACC 
GTGGATGCCA AACTGGGTGG CAAAGCTCCT CTGAAGATTC AAGCTCAGGA CCGTGATGGA 
AACCCAGTCG ATGTGCAGGT GAAGGACAAT GGAAATGGGA CATACAGCTG CAGCTACACC 
CCTCGCAAAC CAGTCAAACA CACTGTGATG GTGTCCTGGG GCGGCGTCAA CATCCCAGAG 
AGCCCGTTCA GAATGAACAT TGGGGCAGGG TGTCATCCCA ATAAGGTGAA AGTATCTGGA 
CCTGGAGTGG CCAAGACAGG CCTCAAAGCA TATGATCCAA CATACTTTAC TGTGGATTGT 
GCTGAGGCTG GACAGGGCGA CATCAGCATA GGCATCAAAT GTGCTGCAGG TGTGGTGGGA 
CCTGCTGAGG CTGACATAGA CTTCGACATC ATTAGAAATG ACAACGACAC ATTTACTGTT 
AAATACACTC CTCCTGGAGC GGGCAGCTAC ACCATCATGG TGCTGTTTGC TGATCAGACC 
ATTCCCATGA CACCCATCAG AATTAAGGTT GATCCCTCTC ATGATGCAAG CAAGGTTAAA 
GCTGAGGGGC CTGGACTTAG CCGTTCTGGT GTGGAGCTGA ACAAACCCAC TCACTTCACT 
GTGAACACAA AGGCTGCAGG CAAGGCCAAG CTTGATGCTC AGTTCACTGG ACCCAACAAG 
GGAGATGCAG TCCGTGACTT CGACATAATC GACAACCATG ATGGCACTCA CACTGTCAAA 
TATACCCCTG TCCAACAGGG CAACATGGGT TTGAATGTCA CCTACGGTGG TGACCCTATT 
CCCAAGAGCC CATTTGCAGT AGCAATTGCC CCCTCTCTGG ACCTCAGTAA AGTTAAAGTG 
GCTGGTCTTG GAAGTAAAAT GACTGTTGGA AAGGATCAGG AGATCACAGT CAAATCTAAA 
GGTGCAGGCG GTCAGGGCAA AGTTGGCGCA AAGGTTACTG GTCCATCTGC TAAATCAGTG 
CCCTGTAAAG TTGAACCAGG CCTGAGCCCA GAAACCAGCC AAGTTCGTTT TATCCCAAGA 
GATAAGGGAC CCTATGAGGT GGAACTGACA TATGATGGCG CCCCCATCCC TGGCAGTCCA 
TTCCCCATCG AAGCCATTGC ACCTACTGAC CCATCCAAGG TGCGATGCTC TGGGCCAGGA 
CTGGAGCGGG CGAAGGTTGG CGAGCCTGGA CAGTTTGTGG TGGATTGCAC TAATGCTGGC 
CCTGCTGAGT TGACCATCGA GATCATCTCT GACAATGGCA CTGAGGCTGA AGTCCACATT 
CAAGACAACG GCGACGGGAC CTACACCATC ACTTACGTCC CTCTCTACCC TGGAGCCTAT 
ACCCTCACCA TCCGCTACGG GGACCAGGAT GTGCCAAACT TCCCAGCAAG ACTCCATGTG 
GAGCCTGCTG TGGAGACCAG TGGAGTGAAA GTGTTCGGAC CCGGAGTGGA GGGCAAAGGT 
GTTTTCAGGG AAGCCACTAC AGATTTCACT GTGGATGCCC GTGCGCTCAC TAAAACCGGA 
GGCAATCACA TCAAAACCTG CATCAACAAC CCATCGGGCA ACCGCACTGA AGCACTGATC 
CGAGATCTGG GAGACGGCAC ATATCAAGTG GAGTACACGC CTTATGAGGA GGGTACACAC 
AGTGTTGAGG TTGCATATGA CAGCTCCCCG GTTCCCAAAA GCCCCTTCCG TGTGCCAGTG 
ACCGAGGGCT GTGACCCGGC GCGTGTGCGT GTGCATGGTC CAGGACTTCA GTCTGGCATC 
ACCAACAAAC CTAACAAATT TACCGTGGAG ACCCGTGGGG CTGGTACTGG TGGACTGGGG 
CTGGCTATGG AAGGACCTTC TGAGGCCAAA ATGTCCTGCA CCGATAACAA AGATGGCAGC 
TGTTGTGTTG AATACATCCC ATATGAGCCA GGGACATACA ACCTCAATGT CACCTATGGA 
GGCCAACCAA TCACTGGAAG CCCTTTCTCT GTACCTGTCC ATGATACTGT TGACCCAACT 
AAAGTGAAAT GTCAGGGCCA GGGGCTTGGA AACAACGTGC GTGCCAATAT CCCACAGGTC 
TTTTCTGTGG ATGCAAGCAA GGCTGGTATG GCGCCACTGC AGGTCAGAGT TCAGGGACCC 
AAAGGTGTTG TGGAGCCTGC TGAGGTGGTG GATAATGGTG ACCAGACTCA CACAGTCAGC 
TATGTGCCCA CAAGAGAGGG GCCATACTCC ATTAATGTTC TGTATGCTGA TGAGGAGATC 
CCACAGAGTC CTTATAAGGT GAAGGTTCTG CCCACTCATG ACGCAAGTAA AGTCCGTTGC 
AGCGGTCCTG GCCTGAATAC CACTGGTGTT CCTGCCAGTC TGCCCGTGGA GTTCACTATT 
GACGCCAAAG ATGCAGGAGA GGGACTGTTG GCCGTCCAGA TAACTGACCC TGATGGGAAG 
CCAAAGAAGG CTAACATTCG TGATAATCAG GACGGGACCT ACCTTGTGTC CTATGTGCCT 
GACATGACCG GCCGTTACAC CATTCTTATT AAGTATGGTG GTGATGAGAT CCCGTATTCT 
CCATATCGCA TTCGAGCCCT GCCTACTGGA GACGCCAGCA AGTGTACAGT CACAGTCTCA 
ATTGGAGGTC ATGGTTTGGG AGCTGGAGTT GGTCCGACTA TTCAGATTGG AGAGCAGACT 
GTCATCACTG TGGATGCGAA GGCAGCTGGG AAGGGCAAGG TGACCTGTAC CGTGTGCACA 
CCTGATGGAG GCGAGGTGGA TGTGGACGTC GTGGAAAATG AGGATGGAAC GTTTGACATC 
TTTTACACTG CACCGCAGCC TGGGAAATAC GTGATCTGTG TGCGCTTCGG TGGAGAGCAC 
ATCCCTAACA GCCCCTTCCA AGTCACGGCT ACAGATGGGC AATTGGGTAT GAATGGACTG 
GATGTAGCTG GACTGAGACC CTTTGACTTG GTCATTCCTT TCACCATTCA GAAAGGAGAA 
ATTACAGGTG ATGTACGAAT GCCCTCTGGA AAAGTGGCCA AGCCTGATAT TACAGACAAC 
AAGGATGGCA CAGTCACAGT GAAATATGCC CCAACTGAGG CGGGCCTGCA TGAGATGGAC 
ATTAAATATG ATGGAATACA CATTCCAGGA AGTCCATTGC AATTCTACGT GGATTATGTC 
AACAGCGGTC ATGTGACTGC ATATGGTCCT GGTCTAATTC ATGGCATGGT CAATAAGCCT 
GCAGTCTTCA CAGTCAACAC AAAGGATGCT GGAGAGGGTG GTCTGTCTCT GGCCATCGAG 
GGGCCATCAA AGGCAGATAT CAGCTGCACT GATAATCAGG ACGGTACCTG CACTGTTTCA 
TACCTCCCTG TTCTTCCCGG AGACTACAAT ATCATTGTTA AATACAATGA CAAGAACATC 
CCCGGCAGCC CTTTCATGTC CAAAATCACA GGTGACGACT CGATGCGCAT GTCTCACCTG 
AAGGTGGGCT CAGCTGCTGA CATCCCTCTG GACATTGGTG AGCTTGACCT CAGTCAGCTG 
ACAGCCACAC TCACCACCCC ATCCGGCCGA GAGGAGCCTT GCCTGCTCAA GATGCTCAGA 
AATGGGCATG TGGGAATTTC CTTTGTGCCC AAGGAAATTG GCGAGCATCT TGTTAACATT 
AAGAAAAATG GACGCCATAT TCCTAGTAGC CCCATTTCTG TCATGATCAA CCAATCAGAG 
ATCGGTGATG CCAGCCGTGT ACGAGTAACT GGTCAAGGCC TGAGTGAAGC TCGCACCTTT 
GAGCCCGCAG AATTCATCAT TGACACTAGA GATGCAGGAT ATGGTGGACT CAGTTTGTCT 
ATTGAGGGAC CTAGTAAAGT GGATATCAAC ACTGAAGATC AGGAAGATGG CACCTGCAAA 
GTGACATATT GCCCCACAGA GCCTGGAAAC TACATAATTA ACATCAAATT TGCAGACCAG 
CATGTGCCAG GAAGTGCTTT CACTGTGAAA GTGACAGGCG AGGGTCGGAT GAAGGAGAGC 
ATCACCCGCA AAAGAAGAGC TGCTTCTGTG GCCAATGTTG GCAGCCAGTG CGACCTCAGT 
CTAAAGATCC CTGAGATCAG CATTGCAGAC ATGACCGCTC AGGTGACCAG CCCATCTGGC 
AAAGTCCACA AGGCAGAGAT AATGGAGGGA GAAAACAACA CCTACTGCAT CCGATTCGTG 
CCCACAGAGA TGGGTGTGCA CACAGTAAGC GTGAAGTATC AAGGTCAGCA TGTGCCTGGA 
TCCCCCTTCC AGTTCACGGT GGGACCTCTT GGAGAAGGAG GAGCTCATAA AGTCAGAGCT 
GGAGGCCCAG GATTAGAGAG GGCAGAAGCT GGTGTACCAG CGGAGTTCAG CATTTGGACT 
CGTGAAGCAG GAGCTGGTGG TCTGTCCATC GCAGTGGAAG GACCCAGCAA AGCAGAGATT 
GCTTTTGAAG ACCGTAAAGA TGGATCCAGT GGTGTCTCTT ACATCGTCCA GGAGCCTGGC 
GATTATGAAG TATCCATCAA GTTCAATGAC GAGCACATCC CTGACAGTCC GTTTGTGGTG 
CCTGTGGCCT CGCCATCTGA CGATGCCCGC CGTCTCACTG TTGCCAGTCT TCAGGAGTCC 
GGGTTAAAGG TGAACCAGCC GGCCTCTTTT GCTGTGAGCC TAAATGGGGC GAAAGGAGTC 
ATCGATGCCA AAGTTCACAG TCCATCAGGA GCACTTGAAG AGTGCTGCGT CACCGAGATC 
GATGAAGATA AATATGCTGT GAGATTCATT CCCAGGGAGA ATGGATTGTA CCTGATAGAT 
GTGAAATTTA ATGGTTCTCA CATCCCTGGA AGCCCCTTCA AGATCCGTGT TGGTGAGACG 
GGTCAGGCTG GAGATCCAGG AATGGTGTCT GCTTATGGAG CTGGTCTGGA AGGAGGCACT 
ACTGGATCGC CTTGTGAGTT CATTGTAAAC ACCAGTTCAG CTGGCCCTGG AGCTTTGGCG 
GTGACTATCG ACGGGCCGTC CAAGGTGAAG ATGGACTGTC AGGAATGCCC AGAGGGCTAC 
AAAGTCACCT ACACACCTAT GGCCCCTGGA AACTACCTAA TTTCTATTAA ATACGGTGGA 
CCGTACCATA TTGTTGGTAG TCCATTCAAA GCTAAAATTA CTGGCTCCCG ATTGGTCAAC 
AGCCACAGCA TGCATGAGAC TTCATCAGTA CTGGTTGACC CTGTGACCCG CAGCATGACG 
ACCAGCCAGC AGGCAGCGCC AGGCTGGGCC AGCTCTGATG CCAGCCGCGT AGTGGCCAAA 
GGTTTGGGGC TCAACAAGGG CTTCATAGGA CAGAAGAACA CTTTCAGTGT GGACTGTAGT 
AAAGCAGGGA GGAACATGCT CCTGGTCGGA GTGGATGGAC CCAAAGTGCC CTGTGAGGAG 
ATCCTGGTGA AACACCTGGG CAACCGCCTC TACAACGTTT CCTACCAGCT GAAGGAGAAA 
GGAGAGTACA TTCTGGTGGT CAAATGGGGT GATGAGCACA TCCCGGGCAG CCCGTATCAC 
ATCACTGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209179.1
CDS	356..7927
Translation	MSQHTRPHQSSAAAPLSNASLSPDKDADMPATEKDLAEDAPWKKIQQNTFTRWCNEHLKCVNKRIANLQTDLSDGLRLIALLEVLSQKKMFRKYNQRPTFRQMQLENVSVALEFLDIENIKLVSIDSKAIVDGNLKLILGLIWTLILHYSISMPMWDEEEESEESKQKTPKQRLLGWIQNKLPELPITNFSRDWQSGKALGALVDSCAPGLCPDWDSWDQTKPVDNAREAMQQADDWLGVPQVITPEEIVDPNVDEHSVMTYLSQFPKAKLKPGAPLRPKLNPKKARAYGPGIEPTGNVVMKKAVFTVETISAGQGEVLVYVEDPAGHREEAKVTANNDKNRTYSVFYVPKVTGQHKVTVLFAGQHISKSPFEVDVGMAQGDSSKVTAQGPGLEPAGNIANKTTYFDVYTAGAGVGEVEVVIMDPSGKKDTVECSVEDKGNSSYRCTYKPTQEGQHIIYITFAGGQISKSPYTVNVGEACNPSLCTAKGRGLQPKGLRVKETAEFKVYTKGAGTGDLKVTIKGPKGLEEPCKKKDLGDGVYSFDYYPSTPGNYIITITWGGQHIPRSPFEVKIGSEAGPQQVRAWGPGLESGVVGKSADFVVEAVGDDVGTLGFSVEGPSPAKIECDDKGDGSCDVRYWPTEPGEYAVHVLCKNEDIQLSPFMAEIMPAPGKDFYPDKVKAYGPGLQSSGLSVGKPAEFTVDAKLGGKAPLKIQAQDRDGNPVDVQVKDNGNGTYSCSYTPRKPVKHTVMVSWGGVNIPESPFRMNIGAGCHPNKVKVSGPGVAKTGLKAYDPTYFTVDCAEAGQGDISIGIKCAAGVVGPAEADIDFDIIRNDNDTFTVKYTPPGAGSYTIMVLFADQTIPMTPIRIKVDPSHDASKVKAEGPGLSRSGVELNKPTHFTVNTKAAGKAKLDAQFTGPNKGDAVRDFDIIDNHDGTHTVKYTPVQQGNMGLNVTYGGDPIPKSPFAVAIAPSLDLSKVKVAGLGSKMTVGKDQEITVKSKGAGGQGKVGAKVTGPSAKSVPCKVEPGLSPETSQVRFIPRDKGPYEVELTYDGAPIPGSPFPIEAIAPTDPSKVRCSGPGLERAKVGEPGQFVVDCTNAGPAELTIEIISDNGTEAEVHIQDNGDGTYTITYVPLYPGAYTLTIRYGDQDVPNFPARLHVEPAVETSGVKVFGPGVEGKGVFREATTDFTVDARALTKTGGNHIKTCINNPSGNRTEALIRDLGDGTYQVEYTPYEEGTHSVEVAYDSSPVPKSPFRVPVTEGCDPARVRVHGPGLQSGITNKPNKFTVETRGAGTGGLGLAMEGPSEAKMSCTDNKDGSCCVEYIPYEPGTYNLNVTYGGQPITGSPFSVPVHDTVDPTKVKCQGQGLGNNVRANIPQVFSVDASKAGMAPLQVRVQGPKGVVEPAEVVDNGDQTHTVSYVPTREGPYSINVLYADEEIPQSPYKVKVLPTHDASKVRCSGPGLNTTGVPASLPVEFTIDAKDAGEGLLAVQITDPDGKPKKANIRDNQDGTYLVSYVPDMTGRYTILIKYGGDEIPYSPYRIRALPTGDASKCTVTVSIGGHGLGAGVGPTIQIGEQTVITVDAKAAGKGKVTCTVCTPDGGEVDVDVVENEDGTFDIFYTAPQPGKYVICVRFGGEHIPNSPFQVTATDGQLGMNGLDVAGLRPFDLVIPFTIQKGEITGDVRMPSGKVAKPDITDNKDGTVTVKYAPTEAGLHEMDIKYDGIHIPGSPLQFYVDYVNSGHVTAYGPGLIHGMVNKPAVFTVNTKDAGEGGLSLAIEGPSKADISCTDNQDGTCTVSYLPVLPGDYNIIVKYNDKNIPGSPFMSKITGDDSMRMSHLKVGSAADIPLDIGELDLSQLTATLTTPSGREEPCLLKMLRNGHVGISFVPKEIGEHLVNIKKNGRHIPSSPISVMINQSEIGDASRVRVTGQGLSEARTFEPAEFIIDTRDAGYGGLSLSIEGPSKVDINTEDQEDGTCKVTYCPTEPGNYIINIKFADQHVPGSAFTVKVTGEGRMKESITRKRRAASVANVGSQCDLSLKIPEISIADMTAQVTSPSGKVHKAEIMEGENNTYCIRFVPTEMGVHTVSVKYQGQHVPGSPFQFTVGPLGEGGAHKVRAGGPGLERAEAGVPAEFSIWTREAGAGGLSIAVEGPSKAEIAFEDRKDGSSGVSYIVQEPGDYEVSIKFNDEHIPDSPFVVPVASPSDDARRLTVASLQESGLKVNQPASFAVSLNGAKGVIDAKVHSPSGALEECCVTEIDEDKYAVRFIPRENGLYLIDVKFNGSHIPGSPFKIRVGETGQAGDPGMVSAYGAGLEGGTTGSPCEFIVNTSSAGPGALAVTIDGPSKVKMDCQECPEGYKVTYTPMAPGNYLISIKYGGPYHIVGSPFKAKITGSRLVNSHSMHETSSVLVDPVTRSMTTSQQAAPGWASSDASRVVAKGLGLNKGFIGQKNTFSVDCSKAGRNMLLVGVDGPKVPCEEILVKHLGNRLYNVSYQLKEKGEYILVVKWGDEHIPGSPYHITV

Target ORF information:

RefSeq Version	XM_017353690.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio filamin A, alpha (actin binding protein 280) (flna), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353690.2

ORF Insert Sequence:

ATGAGTCAAC ACACACGCCC CCACCAGTCG AGCGCCGCCG CTCCCCTATC CAACGCTTCT 
CTCTCCCCGG ACAAGGACGC CGACATGCCC GCTACAGAGA AAGATCTCGC CGAGGACGCG 
CCATGGAAGA AAATCCAGCA AAATACATTC ACGCGTTGGT GCAACGAGCA TCTGAAATGT 
GTCAACAAGC GCATAGCGAA CCTGCAGACG GACCTGAGCG ACGGGCTCCG GCTGATCGCG 
CTTCTGGAGG TGCTCAGCCA GAAGAAGATG TTCCGCAAGT ACAACCAGCG CCCTACCTTC 
AGACAGATGC AGCTGGAGAA CGTCTCCGTG GCGCTGGAGT TCCTGGACAT TGAAAACATC 
AAGCTGGTCT CCATTGACAG TAAGGCCATA GTGGATGGTA ACCTGAAGCT GATCCTGGGT 
CTGATATGGA CTCTTATTCT GCATTATTCC ATCTCCATGC CCATGTGGGA CGAGGAAGAG 
GAGAGCGAGG AAAGCAAGCA GAAGACGCCC AAGCAGAGGC TCCTCGGATG GATCCAGAAC 
AAGCTGCCCG AGCTGCCAAT CACCAACTTC AGCCGCGATT GGCAGTCAGG GAAAGCCCTT 
GGCGCTCTGG TGGACAGTTG TGCTCCAGGT CTGTGTCCAG ACTGGGATTC CTGGGACCAG 
ACCAAGCCTG TGGACAATGC CAGAGAGGCC ATGCAGCAGG CTGATGACTG GCTCGGTGTT 
CCTCAGGTCA TCACACCAGA AGAAATCGTT GATCCCAATG TGGACGAGCA CTCCGTTATG 
ACTTACCTGT CCCAGTTTCC CAAAGCCAAA CTCAAGCCTG GTGCCCCTCT GAGGCCCAAA 
CTCAATCCCA AAAAGGCCCG TGCTTATGGA CCAGGTATTG AGCCAACAGG TAATGTTGTG 
ATGAAGAAGG CTGTGTTCAC TGTTGAGACC ATCAGCGCTG GTCAGGGTGA GGTACTGGTT 
TATGTCGAGG ACCCCGCTGG ACACCGCGAG GAGGCCAAAG TCACAGCCAA CAATGACAAG 
AACCGCACCT ACTCAGTATT CTATGTGCCA AAAGTCACTG GACAACACAA GGTGACAGTG 
CTGTTTGCAG GGCAACACAT CTCTAAGAGC CCGTTTGAGG TGGATGTGGG AATGGCTCAG 
GGAGACTCCA GCAAGGTCAC TGCCCAGGGC CCAGGACTCG AGCCTGCAGG CAACATTGCC 
AACAAAACCA CATACTTTGA TGTCTACACA GCTGGTGCTG GAGTAGGAGA GGTGGAGGTG 
GTCATCATGG ACCCCAGTGG AAAGAAGGAC ACGGTTGAGT GCAGTGTCGA GGATAAAGGC 
AACAGCAGCT ACCGATGCAC CTACAAGCCC ACTCAGGAGG GACAGCACAT CATTTACATC 
ACCTTTGCCG GGGGACAGAT CTCCAAGAGC CCTTACACTG TCAATGTGGG AGAGGCCTGT 
AATCCAAGCC TGTGTACAGC CAAGGGCCGC GGTCTGCAGC CTAAAGGCCT GAGGGTCAAG 
GAGACCGCCG AGTTTAAGGT TTACACCAAA GGAGCAGGAA CCGGAGACCT CAAAGTTACC 
ATCAAAGGGC CCAAGGGTCT CGAGGAGCCC TGTAAGAAGA AGGATTTGGG AGACGGCGTG 
TACAGCTTTG ATTATTACCC CTCCACACCT GGAAATTACA TCATCACCAT CACATGGGGC 
GGTCAACACA TCCCACGCAG CCCGTTTGAG GTGAAGATCG GGTCTGAAGC AGGACCGCAG 
CAGGTCAGGG CGTGGGGTCC AGGACTAGAG AGTGGCGTTG TGGGCAAATC TGCTGACTTT 
GTTGTGGAAG CTGTTGGTGA TGATGTGGGC ACTTTAGGTT TCTCTGTTGA AGGCCCTTCC 
CCGGCTAAGA TTGAATGTGA TGATAAGGGA GATGGTTCAT GTGACGTGAG GTACTGGCCC 
ACCGAACCGG GTGAATATGC AGTTCATGTG CTCTGTAAGA ATGAGGACAT CCAGCTCAGC 
CCTTTCATGG CTGAAATCAT GCCCGCCCCA GGAAAAGACT TTTATCCTGA CAAGGTGAAG 
GCTTATGGCC CCGGTCTGCA GAGCTCAGGC CTGTCAGTTG GCAAACCAGC CGAGTTTACC 
GTGGATGCCA AACTGGGTGG CAAAGCTCCT CTGAAGATTC AAGCTCAGGA CCGTGATGGA 
AACCCAGTCG ATGTGCAGGT GAAGGACAAT GGAAATGGGA CATACAGCTG CAGCTACACC 
CCTCGCAAAC CAGTCAAACA CACTGTGATG GTGTCCTGGG GCGGCGTCAA CATCCCAGAG 
AGCCCGTTCA GAATGAACAT TGGGGCAGGG TGTCATCCCA ATAAGGTGAA AGTATCTGGA 
CCTGGAGTGG CCAAGACAGG CCTCAAAGCA TATGATCCAA CATACTTTAC TGTGGATTGT 
GCTGAGGCTG GACAGGGCGA CATCAGCATA GGCATCAAAT GTGCTGCAGG TGTGGTGGGA 
CCTGCTGAGG CTGACATAGA CTTCGACATC ATTAGAAATG ACAACGACAC ATTTACTGTT 
AAATACACTC CTCCTGGAGC GGGCAGCTAC ACCATCATGG TGCTGTTTGC TGATCAGACC 
ATTCCCATGA CACCCATCAG AATTAAGGTT GATCCCTCTC ATGATGCAAG CAAGGTTAAA 
GCTGAGGGGC CTGGACTTAG CCGTTCTGGT GTGGAGCTGA ACAAACCCAC TCACTTCACT 
GTGAACACAA AGGCTGCAGG CAAGGCCAAG CTTGATGCTC AGTTCACTGG ACCCAACAAG 
GGAGATGCAG TCCGTGACTT CGACATAATC GACAACCATG ATGGCACTCA CACTGTCAAA 
TATACCCCTG TCCAACAGGG CAACATGGGT TTGAATGTCA CCTACGGTGG TGACCCTATT 
CCCAAGAGCC CATTTGCAGT AGCAATTGCC CCCTCTCTGG ACCTCAGTAA AGTTAAAGTG 
GCTGGTCTTG GAAGTAAAAT GACTGTTGGA AAGGATCAGG AGATCACAGT CAAATCTAAA 
GGTGCAGGCG GTCAGGGCAA AGTTGGCGCA AAGGTTACTG GTCCATCTGC TAAATCAGTG 
CCCTGTAAAG TTGAACCAGG CCTGAGCCCA GAAACCAGCC AAGTTCGTTT TATCCCAAGA 
GATAAGGGAC CCTATGAGGT GGAACTGACA TATGATGGCG CCCCCATCCC TGGCAGTCCA 
TTCCCCATCG AAGCCATTGC ACCTACTGAC CCATCCAAGG TGCGATGCTC TGGGCCAGGA 
CTGGAGCGGG CGAAGGTTGG CGAGCCTGGA CAGTTTGTGG TGGATTGCAC TAATGCTGGC 
CCTGCTGAGT TGACCATCGA GATCATCTCT GACAATGGCA CTGAGGCTGA AGTCCACATT 
CAAGACAACG GCGACGGGAC CTACACCATC ACTTACGTCC CTCTCTACCC TGGAGCCTAT 
ACCCTCACCA TCCGCTACGG GGACCAGGAT GTGCCAAACT TCCCAGCAAG ACTCCATGTG 
GAGCCTGCTG TGGAGACCAG TGGAGTGAAA GTGTTCGGAC CCGGAGTGGA GGGCAAAGGT 
GTTTTCAGGG AAGCCACTAC AGATTTCACT GTGGATGCCC GTGCGCTCAC TAAAACCGGA 
GGCAATCACA TCAAAACCTG CATCAACAAC CCATCGGGCA ACCGCACTGA AGCACTGATC 
CGAGATCTGG GAGACGGCAC ATATCAAGTG GAGTACACGC CTTATGAGGA GGGTACACAC 
AGTGTTGAGG TTGCATATGA CAGCTCCCCG GTTCCCAAAA GCCCCTTCCG TGTGCCAGTG 
ACCGAGGGCT GTGACCCGGC GCGTGTGCGT GTGCATGGTC CAGGACTTCA GTCTGGCATC 
ACCAACAAAC CTAACAAATT TACCGTGGAG ACCCGTGGGG CTGGTACTGG TGGACTGGGG 
CTGGCTATGG AAGGACCTTC TGAGGCCAAA ATGTCCTGCA CCGATAACAA AGATGGCAGC 
TGTTGTGTTG AATACATCCC ATATGAGCCA GGGACATACA ACCTCAATGT CACCTATGGA 
GGCCAACCAA TCACTGGAAG CCCTTTCTCT GTACCTGTCC ATGATACTGT TGACCCAACT 
AAAGTGAAAT GTCAGGGCCA GGGGCTTGGA AACAACGTGC GTGCCAATAT CCCACAGGTC 
TTTTCTGTGG ATGCAAGCAA GGCTGGTATG GCGCCACTGC AGGTCAGAGT TCAGGGACCC 
AAAGGTGTTG TGGAGCCTGC TGAGGTGGTG GATAATGGTG ACCAGACTCA CACAGTCAGC 
TATGTGCCCA CAAGAGAGGG GCCATACTCC ATTAATGTTC TGTATGCTGA TGAGGAGATC 
CCACAGAGTC CTTATAAGGT GAAGGTTCTG CCCACTCATG ACGCAAGTAA AGTCCGTTGC 
AGCGGTCCTG GCCTGAATAC CACTGGTGTT CCTGCCAGTC TGCCCGTGGA GTTCACTATT 
GACGCCAAAG ATGCAGGAGA GGGACTGTTG GCCGTCCAGA TAACTGACCC TGATGGGAAG 
CCAAAGAAGG CTAACATTCG TGATAATCAG GACGGGACCT ACCTTGTGTC CTATGTGCCT 
GACATGACCG GCCGTTACAC CATTCTTATT AAGTATGGTG GTGATGAGAT CCCGTATTCT 
CCATATCGCA TTCGAGCCCT GCCTACTGGA GACGCCAGCA AGTGTACAGT CACAGTCTCA 
ATTGGAGGTC ATGGTTTGGG AGCTGGAGTT GGTCCGACTA TTCAGATTGG AGAGCAGACT 
GTCATCACTG TGGATGCGAA GGCAGCTGGG AAGGGCAAGG TGACCTGTAC CGTGTGCACA 
CCTGATGGAG GCGAGGTGGA TGTGGACGTC GTGGAAAATG AGGATGGAAC GTTTGACATC 
TTTTACACTG CACCGCAGCC TGGGAAATAC GTGATCTGTG TGCGCTTCGG TGGAGAGCAC 
ATCCCTAACA GCCCCTTCCA AGTCACGGCT ACAGATGGGC AATTGGGTAT GAATGGACTG 
GATGTAGCTG GACTGAGACC CTTTGACTTG GTCATTCCTT TCACCATTCA GAAAGGAGAA 
ATTACAGGTG ATGTACGAAT GCCCTCTGGA AAAGTGGCCA AGCCTGATAT TACAGACAAC 
AAGGATGGCA CAGTCACAGT GAAATATGCC CCAACTGAGG CGGGCCTGCA TGAGATGGAC 
ATTAAATATG ATGGAATACA CATTCCAGGA AGTCCATTGC AATTCTACGT GGATTATGTC 
AACAGCGGTC ATGTGACTGC ATATGGTCCT GGTCTAATTC ATGGCATGGT CAATAAGCCT 
GCAGTCTTCA CAGTCAACAC AAAGGATGCT GGAGAGGGTG GTCTGTCTCT GGCCATCGAG 
GGGCCATCAA AGGCAGATAT CAGCTGCACT GATAATCAGG ACGGTACCTG CACTGTTTCA 
TACCTCCCTG TTCTTCCCGG AGACTACAAT ATCATTGTTA AATACAATGA CAAGAACATC 
CCCGGCAGCC CTTTCATGTC CAAAATCACA GGTGACGACT CGATGCGCAT GTCTCACCTG 
AAGGTGGGCT CAGCTGCTGA CATCCCTCTG GACATTGGTG AGCTTGACCT CAGTCAGCTG 
ACAGCCACAC TCACCACCCC ATCCGGCCGA GAGGAGCCTT GCCTGCTCAA GATGCTCAGA 
AATGGGCATG TGGGAATTTC CTTTGTGCCC AAGGAAATTG GCGAGCATCT TGTTAACATT 
AAGAAAAATG GACGCCATAT TCCTAGTAGC CCCATTTCTG TCATGATCAA CCAATCAGAG 
ATCGGTGATG CCAGCCGTGT ACGAGTAACT GGTCAAGGCC TGAGTGAAGC TCGCACCTTT 
GAGCCCGCAG AATTCATCAT TGACACTAGA GATGCAGGAT ATGGTGGACT CAGTTTGTCT 
ATTGAGGGAC CTAGTAAAGT GGATATCAAC ACTGAAGATC AGGAAGATGG CACCTGCAAA 
GTGACATATT GCCCCACAGA GCCTGGAAAC TACATAATTA ACATCAAATT TGCAGACCAG 
CATGTGCCAG GAAGTGCTTT CACTGTGAAA GTGACAGGCG AGGGTCGGAT GAAGGAGAGC 
ATCACCCGCA AAAGAAGAGC TGCTTCTGTG GCCAATGTTG GCAGCCAGTG CGACCTCAGT 
CTAAAGATCC CTGAGATCAG CATTGCAGAC ATGACCGCTC AGGTGACCAG CCCATCTGGC 
AAAGTCCACA AGGCAGAGAT AATGGAGGGA GAAAACAACA CCTACTGCAT CCGATTCGTG 
CCCACAGAGA TGGGTGTGCA CACAGTAAGC GTGAAGTATC AAGGTCAGCA TGTGCCTGGA 
TCCCCCTTCC AGTTCACGGT GGGACCTCTT GGAGAAGGAG GAGCTCATAA AGTCAGAGCT 
GGAGGCCCAG GATTAGAGAG GGCAGAAGCT GGTGTACCAG CGGAGTTCAG CATTTGGACT 
CGTGAAGCAG GAGCTGGTGG TCTGTCCATC GCAGTGGAAG GACCCAGCAA AGCAGAGATT 
GCTTTTGAAG ACCGTAAAGA TGGATCCAGT GGTGTCTCTT ACATCGTCCA GGAGCCTGGC 
GATTATGAAG TATCCATCAA GTTCAATGAC GAGCACATCC CTGACAGTCC GTTTGTGGTG 
CCTGTGGCCT CGCCATCTGA CGATGCCCGC CGTCTCACTG TTGCCAGTCT TCAGGAGTCC 
GGGTTAAAGG TGAACCAGCC GGCCTCTTTT GCTGTGAGCC TAAATGGGGC GAAAGGAGTC 
ATCGATGCCA AAGTTCACAG TCCATCAGGA GCACTTGAAG AGTGCTGCGT CACCGAGATC 
GATGAAGATA AATATGCTGT GAGATTCATT CCCAGGGAGA ATGGATTGTA CCTGATAGAT 
GTGAAATTTA ATGGTTCTCA CATCCCTGGA AGCCCCTTCA AGATCCGTGT TGGTGAGACG 
GGTCAGGCTG GAGATCCAGG AATGGTGTCT GCTTATGGAG CTGGTCTGGA AGGAGGCACT 
ACTGGATCGC CTTGTGAGTT CATTGTAAAC ACCAGTTCAG CTGGCCCTGG AGCTTTGGCG 
GTGACTATCG ACGGGCCGTC CAAGGTGAAG ATGGACTGTC AGGAATGCCC AGAGGGCTAC 
AAAGTCACCT ACACACCTAT GGCCCCTGGA AACTACCTAA TTTCTATTAA ATACGGTGGA 
CCGTACCATA TTGTTGGTAG TCCATTCAAA GCTAAAATTA CTGGCTCCCG ATTGGTCAAC 
AGCCACAGCA TGCATGAGAC TTCATCAGTA CTGGTTGACC CTGTGACCCG CAGCATGACG 
ACCAGCCAGC AGGCAGCGCC AGGCTGGGCC AGCTCTGATG CCAGCCGCGT AGTGGCCAAA 
GGTTTGGGGC TCAACAAGGG CTTCATAGGA CAGAAGAACA CTTTCAGTGT GGACTGTAGT 
AAAGCAGGGA GGAACATGCT CCTGGTCGGA GTGGATGGAC CCAAAGTGCC CTGTGAGGAG 
ATCCTGGTGA AACACCTGGG CAACCGCCTC TACAACGTTT CCTACCAGCT GAAGGAGAAA 
GGAGAGTACA TTCTGGTGGT CAAATGGGGT GATGAGCACA TCCCGGGCAG CCCGTATCAC 
ATCACTGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

flna ( XM_017353690.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017209179.1 Danio rerio filamin A, alpha (actin binding protein 280) (flna), transcript variant X3, mRNA.

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CloneID	ODa57477
Clone ID Related Accession (Same CDS sequence)	XM_021474525.1 , XM_017353690.2 , XM_017353690.1
Accession Version	XM_017353690.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7572bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	filamin-A isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCAAC ACACACGCCC CCACCAGTCG AGCGCCGCCG CTCCCCTATC CAACGCTTCT 
CTCTCCCCGG ACAAGGACGC CGACATGCCC GCTACAGAGA AAGATCTCGC CGAGGACGCG 
CCATGGAAGA AAATCCAGCA AAATACATTC ACGCGTTGGT GCAACGAGCA TCTGAAATGT 
GTCAACAAGC GCATAGCGAA CCTGCAGACG GACCTGAGCG ACGGGCTCCG GCTGATCGCG 
CTTCTGGAGG TGCTCAGCCA GAAGAAGATG TTCCGCAAGT ACAACCAGCG CCCTACCTTC 
AGACAGATGC AGCTGGAGAA CGTCTCCGTG GCGCTGGAGT TCCTGGACAT TGAAAACATC 
AAGCTGGTCT CCATTGACAG TAAGGCCATA GTGGATGGTA ACCTGAAGCT GATCCTGGGT 
CTGATATGGA CTCTTATTCT GCATTATTCC ATCTCCATGC CCATGTGGGA CGAGGAAGAG 
GAGAGCGAGG AAAGCAAGCA GAAGACGCCC AAGCAGAGGC TCCTCGGATG GATCCAGAAC 
AAGCTGCCCG AGCTGCCAAT CACCAACTTC AGCCGCGATT GGCAGTCAGG GAAAGCCCTT 
GGCGCTCTGG TGGACAGTTG TGCTCCAGGT CTGTGTCCAG ACTGGGATTC CTGGGACCAG 
ACCAAGCCTG TGGACAATGC CAGAGAGGCC ATGCAGCAGG CTGATGACTG GCTCGGTGTT 
CCTCAGGTCA TCACACCAGA AGAAATCGTT GATCCCAATG TGGACGAGCA CTCCGTTATG 
ACTTACCTGT CCCAGTTTCC CAAAGCCAAA CTCAAGCCTG GTGCCCCTCT GAGGCCCAAA 
CTCAATCCCA AAAAGGCCCG TGCTTATGGA CCAGGTATTG AGCCAACAGG TAATGTTGTG 
ATGAAGAAGG CTGTGTTCAC TGTTGAGACC ATCAGCGCTG GTCAGGGTGA GGTACTGGTT 
TATGTCGAGG ACCCCGCTGG ACACCGCGAG GAGGCCAAAG TCACAGCCAA CAATGACAAG 
AACCGCACCT ACTCAGTATT CTATGTGCCA AAAGTCACTG GACAACACAA GGTGACAGTG 
CTGTTTGCAG GGCAACACAT CTCTAAGAGC CCGTTTGAGG TGGATGTGGG AATGGCTCAG 
GGAGACTCCA GCAAGGTCAC TGCCCAGGGC CCAGGACTCG AGCCTGCAGG CAACATTGCC 
AACAAAACCA CATACTTTGA TGTCTACACA GCTGGTGCTG GAGTAGGAGA GGTGGAGGTG 
GTCATCATGG ACCCCAGTGG AAAGAAGGAC ACGGTTGAGT GCAGTGTCGA GGATAAAGGC 
AACAGCAGCT ACCGATGCAC CTACAAGCCC ACTCAGGAGG GACAGCACAT CATTTACATC 
ACCTTTGCCG GGGGACAGAT CTCCAAGAGC CCTTACACTG TCAATGTGGG AGAGGCCTGT 
AATCCAAGCC TGTGTACAGC CAAGGGCCGC GGTCTGCAGC CTAAAGGCCT GAGGGTCAAG 
GAGACCGCCG AGTTTAAGGT TTACACCAAA GGAGCAGGAA CCGGAGACCT CAAAGTTACC 
ATCAAAGGGC CCAAGGGTCT CGAGGAGCCC TGTAAGAAGA AGGATTTGGG AGACGGCGTG 
TACAGCTTTG ATTATTACCC CTCCACACCT GGAAATTACA TCATCACCAT CACATGGGGC 
GGTCAACACA TCCCACGCAG CCCGTTTGAG GTGAAGATCG GGTCTGAAGC AGGACCGCAG 
CAGGTCAGGG CGTGGGGTCC AGGACTAGAG AGTGGCGTTG TGGGCAAATC TGCTGACTTT 
GTTGTGGAAG CTGTTGGTGA TGATGTGGGC ACTTTAGGTT TCTCTGTTGA AGGCCCTTCC 
CCGGCTAAGA TTGAATGTGA TGATAAGGGA GATGGTTCAT GTGACGTGAG GTACTGGCCC 
ACCGAACCGG GTGAATATGC AGTTCATGTG CTCTGTAAGA ATGAGGACAT CCAGCTCAGC 
CCTTTCATGG CTGAAATCAT GCCCGCCCCA GGAAAAGACT TTTATCCTGA CAAGGTGAAG 
GCTTATGGCC CCGGTCTGCA GAGCTCAGGC CTGTCAGTTG GCAAACCAGC CGAGTTTACC 
GTGGATGCCA AACTGGGTGG CAAAGCTCCT CTGAAGATTC AAGCTCAGGA CCGTGATGGA 
AACCCAGTCG ATGTGCAGGT GAAGGACAAT GGAAATGGGA CATACAGCTG CAGCTACACC 
CCTCGCAAAC CAGTCAAACA CACTGTGATG GTGTCCTGGG GCGGCGTCAA CATCCCAGAG 
AGCCCGTTCA GAATGAACAT TGGGGCAGGG TGTCATCCCA ATAAGGTGAA AGTATCTGGA 
CCTGGAGTGG CCAAGACAGG CCTCAAAGCA TATGATCCAA CATACTTTAC TGTGGATTGT 
GCTGAGGCTG GACAGGGCGA CATCAGCATA GGCATCAAAT GTGCTGCAGG TGTGGTGGGA 
CCTGCTGAGG CTGACATAGA CTTCGACATC ATTAGAAATG ACAACGACAC ATTTACTGTT 
AAATACACTC CTCCTGGAGC GGGCAGCTAC ACCATCATGG TGCTGTTTGC TGATCAGACC 
ATTCCCATGA CACCCATCAG AATTAAGGTT GATCCCTCTC ATGATGCAAG CAAGGTTAAA 
GCTGAGGGGC CTGGACTTAG CCGTTCTGGT GTGGAGCTGA ACAAACCCAC TCACTTCACT 
GTGAACACAA AGGCTGCAGG CAAGGCCAAG CTTGATGCTC AGTTCACTGG ACCCAACAAG 
GGAGATGCAG TCCGTGACTT CGACATAATC GACAACCATG ATGGCACTCA CACTGTCAAA 
TATACCCCTG TCCAACAGGG CAACATGGGT TTGAATGTCA CCTACGGTGG TGACCCTATT 
CCCAAGAGCC CATTTGCAGT AGCAATTGCC CCCTCTCTGG ACCTCAGTAA AGTTAAAGTG 
GCTGGTCTTG GAAGTAAAAT GACTGTTGGA AAGGATCAGG AGATCACAGT CAAATCTAAA 
GGTGCAGGCG GTCAGGGCAA AGTTGGCGCA AAGGTTACTG GTCCATCTGC TAAATCAGTG 
CCCTGTAAAG TTGAACCAGG CCTGAGCCCA GAAACCAGCC AAGTTCGTTT TATCCCAAGA 
GATAAGGGAC CCTATGAGGT GGAACTGACA TATGATGGCG CCCCCATCCC TGGCAGTCCA 
TTCCCCATCG AAGCCATTGC ACCTACTGAC CCATCCAAGG TGCGATGCTC TGGGCCAGGA 
CTGGAGCGGG CGAAGGTTGG CGAGCCTGGA CAGTTTGTGG TGGATTGCAC TAATGCTGGC 
CCTGCTGAGT TGACCATCGA GATCATCTCT GACAATGGCA CTGAGGCTGA AGTCCACATT 
CAAGACAACG GCGACGGGAC CTACACCATC ACTTACGTCC CTCTCTACCC TGGAGCCTAT 
ACCCTCACCA TCCGCTACGG GGACCAGGAT GTGCCAAACT TCCCAGCAAG ACTCCATGTG 
GAGCCTGCTG TGGAGACCAG TGGAGTGAAA GTGTTCGGAC CCGGAGTGGA GGGCAAAGGT 
GTTTTCAGGG AAGCCACTAC AGATTTCACT GTGGATGCCC GTGCGCTCAC TAAAACCGGA 
GGCAATCACA TCAAAACCTG CATCAACAAC CCATCGGGCA ACCGCACTGA AGCACTGATC 
CGAGATCTGG GAGACGGCAC ATATCAAGTG GAGTACACGC CTTATGAGGA GGGTACACAC 
AGTGTTGAGG TTGCATATGA CAGCTCCCCG GTTCCCAAAA GCCCCTTCCG TGTGCCAGTG 
ACCGAGGGCT GTGACCCGGC GCGTGTGCGT GTGCATGGTC CAGGACTTCA GTCTGGCATC 
ACCAACAAAC CTAACAAATT TACCGTGGAG ACCCGTGGGG CTGGTACTGG TGGACTGGGG 
CTGGCTATGG AAGGACCTTC TGAGGCCAAA ATGTCCTGCA CCGATAACAA AGATGGCAGC 
TGTTGTGTTG AATACATCCC ATATGAGCCA GGGACATACA ACCTCAATGT CACCTATGGA 
GGCCAACCAA TCACTGGAAG CCCTTTCTCT GTACCTGTCC ATGATACTGT TGACCCAACT 
AAAGTGAAAT GTCAGGGCCA GGGGCTTGGA AACAACGTGC GTGCCAATAT CCCACAGGTC 
TTTTCTGTGG ATGCAAGCAA GGCTGGTATG GCGCCACTGC AGGTCAGAGT TCAGGGACCC 
AAAGGTGTTG TGGAGCCTGC TGAGGTGGTG GATAATGGTG ACCAGACTCA CACAGTCAGC 
TATGTGCCCA CAAGAGAGGG GCCATACTCC ATTAATGTTC TGTATGCTGA TGAGGAGATC 
CCACAGAGTC CTTATAAGGT GAAGGTTCTG CCCACTCATG ACGCAAGTAA AGTCCGTTGC 
AGCGGTCCTG GCCTGAATAC CACTGGTGTT CCTGCCAGTC TGCCCGTGGA GTTCACTATT 
GACGCCAAAG ATGCAGGAGA GGGACTGTTG GCCGTCCAGA TAACTGACCC TGATGGGAAG 
CCAAAGAAGG CTAACATTCG TGATAATCAG GACGGGACCT ACCTTGTGTC CTATGTGCCT 
GACATGACCG GCCGTTACAC CATTCTTATT AAGTATGGTG GTGATGAGAT CCCGTATTCT 
CCATATCGCA TTCGAGCCCT GCCTACTGGA GACGCCAGCA AGTGTACAGT CACAGTCTCA 
ATTGGAGGTC ATGGTTTGGG AGCTGGAGTT GGTCCGACTA TTCAGATTGG AGAGCAGACT 
GTCATCACTG TGGATGCGAA GGCAGCTGGG AAGGGCAAGG TGACCTGTAC CGTGTGCACA 
CCTGATGGAG GCGAGGTGGA TGTGGACGTC GTGGAAAATG AGGATGGAAC GTTTGACATC 
TTTTACACTG CACCGCAGCC TGGGAAATAC GTGATCTGTG TGCGCTTCGG TGGAGAGCAC 
ATCCCTAACA GCCCCTTCCA AGTCACGGCT ACAGATGGGC AATTGGGTAT GAATGGACTG 
GATGTAGCTG GACTGAGACC CTTTGACTTG GTCATTCCTT TCACCATTCA GAAAGGAGAA 
ATTACAGGTG ATGTACGAAT GCCCTCTGGA AAAGTGGCCA AGCCTGATAT TACAGACAAC 
AAGGATGGCA CAGTCACAGT GAAATATGCC CCAACTGAGG CGGGCCTGCA TGAGATGGAC 
ATTAAATATG ATGGAATACA CATTCCAGGA AGTCCATTGC AATTCTACGT GGATTATGTC 
AACAGCGGTC ATGTGACTGC ATATGGTCCT GGTCTAATTC ATGGCATGGT CAATAAGCCT 
GCAGTCTTCA CAGTCAACAC AAAGGATGCT GGAGAGGGTG GTCTGTCTCT GGCCATCGAG 
GGGCCATCAA AGGCAGATAT CAGCTGCACT GATAATCAGG ACGGTACCTG CACTGTTTCA 
TACCTCCCTG TTCTTCCCGG AGACTACAAT ATCATTGTTA AATACAATGA CAAGAACATC 
CCCGGCAGCC CTTTCATGTC CAAAATCACA GGTGACGACT CGATGCGCAT GTCTCACCTG 
AAGGTGGGCT CAGCTGCTGA CATCCCTCTG GACATTGGTG AGCTTGACCT CAGTCAGCTG 
ACAGCCACAC TCACCACCCC ATCCGGCCGA GAGGAGCCTT GCCTGCTCAA GATGCTCAGA 
AATGGGCATG TGGGAATTTC CTTTGTGCCC AAGGAAATTG GCGAGCATCT TGTTAACATT 
AAGAAAAATG GACGCCATAT TCCTAGTAGC CCCATTTCTG TCATGATCAA CCAATCAGAG 
ATCGGTGATG CCAGCCGTGT ACGAGTAACT GGTCAAGGCC TGAGTGAAGC TCGCACCTTT 
GAGCCCGCAG AATTCATCAT TGACACTAGA GATGCAGGAT ATGGTGGACT CAGTTTGTCT 
ATTGAGGGAC CTAGTAAAGT GGATATCAAC ACTGAAGATC AGGAAGATGG CACCTGCAAA 
GTGACATATT GCCCCACAGA GCCTGGAAAC TACATAATTA ACATCAAATT TGCAGACCAG 
CATGTGCCAG GAAGTGCTTT CACTGTGAAA GTGACAGGCG AGGGTCGGAT GAAGGAGAGC 
ATCACCCGCA AAAGAAGAGC TGCTTCTGTG GCCAATGTTG GCAGCCAGTG CGACCTCAGT 
CTAAAGATCC CTGAGATCAG CATTGCAGAC ATGACCGCTC AGGTGACCAG CCCATCTGGC 
AAAGTCCACA AGGCAGAGAT AATGGAGGGA GAAAACAACA CCTACTGCAT CCGATTCGTG 
CCCACAGAGA TGGGTGTGCA CACAGTAAGC GTGAAGTATC AAGGTCAGCA TGTGCCTGGA 
TCCCCCTTCC AGTTCACGGT GGGACCTCTT GGAGAAGGAG GAGCTCATAA AGTCAGAGCT 
GGAGGCCCAG GATTAGAGAG GGCAGAAGCT GGTGTACCAG CGGAGTTCAG CATTTGGACT 
CGTGAAGCAG GAGCTGGTGG TCTGTCCATC GCAGTGGAAG GACCCAGCAA AGCAGAGATT 
GCTTTTGAAG ACCGTAAAGA TGGATCCAGT GGTGTCTCTT ACATCGTCCA GGAGCCTGGC 
GATTATGAAG TATCCATCAA GTTCAATGAC GAGCACATCC CTGACAGTCC GTTTGTGGTG 
CCTGTGGCCT CGCCATCTGA CGATGCCCGC CGTCTCACTG TTGCCAGTCT TCAGGAGTCC 
GGGTTAAAGG TGAACCAGCC GGCCTCTTTT GCTGTGAGCC TAAATGGGGC GAAAGGAGTC 
ATCGATGCCA AAGTTCACAG TCCATCAGGA GCACTTGAAG AGTGCTGCGT CACCGAGATC 
GATGAAGATA AATATGCTGT GAGATTCATT CCCAGGGAGA ATGGATTGTA CCTGATAGAT 
GTGAAATTTA ATGGTTCTCA CATCCCTGGA AGCCCCTTCA AGATCCGTGT TGGTGAGACG 
GGTCAGGCTG GAGATCCAGG AATGGTGTCT GCTTATGGAG CTGGTCTGGA AGGAGGCACT 
ACTGGATCGC CTTGTGAGTT CATTGTAAAC ACCAGTTCAG CTGGCCCTGG AGCTTTGGCG 
GTGACTATCG ACGGGCCGTC CAAGGTGAAG ATGGACTGTC AGGAATGCCC AGAGGGCTAC 
AAAGTCACCT ACACACCTAT GGCCCCTGGA AACTACCTAA TTTCTATTAA ATACGGTGGA 
CCGTACCATA TTGTTGGTAG TCCATTCAAA GCTAAAATTA CTGGCTCCCG ATTGGTCAAC 
AGCCACAGCA TGCATGAGAC TTCATCAGTA CTGGTTGACC CTGTGACCCG CAGCATGACG 
ACCAGCCAGC AGGCAGCGCC AGGCTGGGCC AGCTCTGATG CCAGCCGCGT AGTGGCCAAA 
GGTTTGGGGC TCAACAAGGG CTTCATAGGA CAGAAGAACA CTTTCAGTGT GGACTGTAGT 
AAAGCAGGGA GGAACATGCT CCTGGTCGGA GTGGATGGAC CCAAAGTGCC CTGTGAGGAG 
ATCCTGGTGA AACACCTGGG CAACCGCCTC TACAACGTTT CCTACCAGCT GAAGGAGAAA 
GGAGAGTACA TTCTGGTGGT CAAATGGGGT GATGAGCACA TCCCGGGCAG CCCGTATCAC 
ATCACTGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017209179.1
CDS	370..7941
Translation	MSQHTRPHQSSAAAPLSNASLSPDKDADMPATEKDLAEDAPWKKIQQNTFTRWCNEHLKCVNKRIANLQTDLSDGLRLIALLEVLSQKKMFRKYNQRPTFRQMQLENVSVALEFLDIENIKLVSIDSKAIVDGNLKLILGLIWTLILHYSISMPMWDEEEESEESKQKTPKQRLLGWIQNKLPELPITNFSRDWQSGKALGALVDSCAPGLCPDWDSWDQTKPVDNAREAMQQADDWLGVPQVITPEEIVDPNVDEHSVMTYLSQFPKAKLKPGAPLRPKLNPKKARAYGPGIEPTGNVVMKKAVFTVETISAGQGEVLVYVEDPAGHREEAKVTANNDKNRTYSVFYVPKVTGQHKVTVLFAGQHISKSPFEVDVGMAQGDSSKVTAQGPGLEPAGNIANKTTYFDVYTAGAGVGEVEVVIMDPSGKKDTVECSVEDKGNSSYRCTYKPTQEGQHIIYITFAGGQISKSPYTVNVGEACNPSLCTAKGRGLQPKGLRVKETAEFKVYTKGAGTGDLKVTIKGPKGLEEPCKKKDLGDGVYSFDYYPSTPGNYIITITWGGQHIPRSPFEVKIGSEAGPQQVRAWGPGLESGVVGKSADFVVEAVGDDVGTLGFSVEGPSPAKIECDDKGDGSCDVRYWPTEPGEYAVHVLCKNEDIQLSPFMAEIMPAPGKDFYPDKVKAYGPGLQSSGLSVGKPAEFTVDAKLGGKAPLKIQAQDRDGNPVDVQVKDNGNGTYSCSYTPRKPVKHTVMVSWGGVNIPESPFRMNIGAGCHPNKVKVSGPGVAKTGLKAYDPTYFTVDCAEAGQGDISIGIKCAAGVVGPAEADIDFDIIRNDNDTFTVKYTPPGAGSYTIMVLFADQTIPMTPIRIKVDPSHDASKVKAEGPGLSRSGVELNKPTHFTVNTKAAGKAKLDAQFTGPNKGDAVRDFDIIDNHDGTHTVKYTPVQQGNMGLNVTYGGDPIPKSPFAVAIAPSLDLSKVKVAGLGSKMTVGKDQEITVKSKGAGGQGKVGAKVTGPSAKSVPCKVEPGLSPETSQVRFIPRDKGPYEVELTYDGAPIPGSPFPIEAIAPTDPSKVRCSGPGLERAKVGEPGQFVVDCTNAGPAELTIEIISDNGTEAEVHIQDNGDGTYTITYVPLYPGAYTLTIRYGDQDVPNFPARLHVEPAVETSGVKVFGPGVEGKGVFREATTDFTVDARALTKTGGNHIKTCINNPSGNRTEALIRDLGDGTYQVEYTPYEEGTHSVEVAYDSSPVPKSPFRVPVTEGCDPARVRVHGPGLQSGITNKPNKFTVETRGAGTGGLGLAMEGPSEAKMSCTDNKDGSCCVEYIPYEPGTYNLNVTYGGQPITGSPFSVPVHDTVDPTKVKCQGQGLGNNVRANIPQVFSVDASKAGMAPLQVRVQGPKGVVEPAEVVDNGDQTHTVSYVPTREGPYSINVLYADEEIPQSPYKVKVLPTHDASKVRCSGPGLNTTGVPASLPVEFTIDAKDAGEGLLAVQITDPDGKPKKANIRDNQDGTYLVSYVPDMTGRYTILIKYGGDEIPYSPYRIRALPTGDASKCTVTVSIGGHGLGAGVGPTIQIGEQTVITVDAKAAGKGKVTCTVCTPDGGEVDVDVVENEDGTFDIFYTAPQPGKYVICVRFGGEHIPNSPFQVTATDGQLGMNGLDVAGLRPFDLVIPFTIQKGEITGDVRMPSGKVAKPDITDNKDGTVTVKYAPTEAGLHEMDIKYDGIHIPGSPLQFYVDYVNSGHVTAYGPGLIHGMVNKPAVFTVNTKDAGEGGLSLAIEGPSKADISCTDNQDGTCTVSYLPVLPGDYNIIVKYNDKNIPGSPFMSKITGDDSMRMSHLKVGSAADIPLDIGELDLSQLTATLTTPSGREEPCLLKMLRNGHVGISFVPKEIGEHLVNIKKNGRHIPSSPISVMINQSEIGDASRVRVTGQGLSEARTFEPAEFIIDTRDAGYGGLSLSIEGPSKVDINTEDQEDGTCKVTYCPTEPGNYIINIKFADQHVPGSAFTVKVTGEGRMKESITRKRRAASVANVGSQCDLSLKIPEISIADMTAQVTSPSGKVHKAEIMEGENNTYCIRFVPTEMGVHTVSVKYQGQHVPGSPFQFTVGPLGEGGAHKVRAGGPGLERAEAGVPAEFSIWTREAGAGGLSIAVEGPSKAEIAFEDRKDGSSGVSYIVQEPGDYEVSIKFNDEHIPDSPFVVPVASPSDDARRLTVASLQESGLKVNQPASFAVSLNGAKGVIDAKVHSPSGALEECCVTEIDEDKYAVRFIPRENGLYLIDVKFNGSHIPGSPFKIRVGETGQAGDPGMVSAYGAGLEGGTTGSPCEFIVNTSSAGPGALAVTIDGPSKVKMDCQECPEGYKVTYTPMAPGNYLISIKYGGPYHIVGSPFKAKITGSRLVNSHSMHETSSVLVDPVTRSMTTSQQAAPGWASSDASRVVAKGLGLNKGFIGQKNTFSVDCSKAGRNMLLVGVDGPKVPCEEILVKHLGNRLYNVSYQLKEKGEYILVVKWGDEHIPGSPYHITV

Target ORF information:

RefSeq Version	XM_017353690.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio filamin A, alpha (actin binding protein 280) (flna), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017353690.1

ORF Insert Sequence:

ATGAGTCAAC ACACACGCCC CCACCAGTCG AGCGCCGCCG CTCCCCTATC CAACGCTTCT 
CTCTCCCCGG ACAAGGACGC CGACATGCCC GCTACAGAGA AAGATCTCGC CGAGGACGCG 
CCATGGAAGA AAATCCAGCA AAATACATTC ACGCGTTGGT GCAACGAGCA TCTGAAATGT 
GTCAACAAGC GCATAGCGAA CCTGCAGACG GACCTGAGCG ACGGGCTCCG GCTGATCGCG 
CTTCTGGAGG TGCTCAGCCA GAAGAAGATG TTCCGCAAGT ACAACCAGCG CCCTACCTTC 
AGACAGATGC AGCTGGAGAA CGTCTCCGTG GCGCTGGAGT TCCTGGACAT TGAAAACATC 
AAGCTGGTCT CCATTGACAG TAAGGCCATA GTGGATGGTA ACCTGAAGCT GATCCTGGGT 
CTGATATGGA CTCTTATTCT GCATTATTCC ATCTCCATGC CCATGTGGGA CGAGGAAGAG 
GAGAGCGAGG AAAGCAAGCA GAAGACGCCC AAGCAGAGGC TCCTCGGATG GATCCAGAAC 
AAGCTGCCCG AGCTGCCAAT CACCAACTTC AGCCGCGATT GGCAGTCAGG GAAAGCCCTT 
GGCGCTCTGG TGGACAGTTG TGCTCCAGGT CTGTGTCCAG ACTGGGATTC CTGGGACCAG 
ACCAAGCCTG TGGACAATGC CAGAGAGGCC ATGCAGCAGG CTGATGACTG GCTCGGTGTT 
CCTCAGGTCA TCACACCAGA AGAAATCGTT GATCCCAATG TGGACGAGCA CTCCGTTATG 
ACTTACCTGT CCCAGTTTCC CAAAGCCAAA CTCAAGCCTG GTGCCCCTCT GAGGCCCAAA 
CTCAATCCCA AAAAGGCCCG TGCTTATGGA CCAGGTATTG AGCCAACAGG TAATGTTGTG 
ATGAAGAAGG CTGTGTTCAC TGTTGAGACC ATCAGCGCTG GTCAGGGTGA GGTACTGGTT 
TATGTCGAGG ACCCCGCTGG ACACCGCGAG GAGGCCAAAG TCACAGCCAA CAATGACAAG 
AACCGCACCT ACTCAGTATT CTATGTGCCA AAAGTCACTG GACAACACAA GGTGACAGTG 
CTGTTTGCAG GGCAACACAT CTCTAAGAGC CCGTTTGAGG TGGATGTGGG AATGGCTCAG 
GGAGACTCCA GCAAGGTCAC TGCCCAGGGC CCAGGACTCG AGCCTGCAGG CAACATTGCC 
AACAAAACCA CATACTTTGA TGTCTACACA GCTGGTGCTG GAGTAGGAGA GGTGGAGGTG 
GTCATCATGG ACCCCAGTGG AAAGAAGGAC ACGGTTGAGT GCAGTGTCGA GGATAAAGGC 
AACAGCAGCT ACCGATGCAC CTACAAGCCC ACTCAGGAGG GACAGCACAT CATTTACATC 
ACCTTTGCCG GGGGACAGAT CTCCAAGAGC CCTTACACTG TCAATGTGGG AGAGGCCTGT 
AATCCAAGCC TGTGTACAGC CAAGGGCCGC GGTCTGCAGC CTAAAGGCCT GAGGGTCAAG 
GAGACCGCCG AGTTTAAGGT TTACACCAAA GGAGCAGGAA CCGGAGACCT CAAAGTTACC 
ATCAAAGGGC CCAAGGGTCT CGAGGAGCCC TGTAAGAAGA AGGATTTGGG AGACGGCGTG 
TACAGCTTTG ATTATTACCC CTCCACACCT GGAAATTACA TCATCACCAT CACATGGGGC 
GGTCAACACA TCCCACGCAG CCCGTTTGAG GTGAAGATCG GGTCTGAAGC AGGACCGCAG 
CAGGTCAGGG CGTGGGGTCC AGGACTAGAG AGTGGCGTTG TGGGCAAATC TGCTGACTTT 
GTTGTGGAAG CTGTTGGTGA TGATGTGGGC ACTTTAGGTT TCTCTGTTGA AGGCCCTTCC 
CCGGCTAAGA TTGAATGTGA TGATAAGGGA GATGGTTCAT GTGACGTGAG GTACTGGCCC 
ACCGAACCGG GTGAATATGC AGTTCATGTG CTCTGTAAGA ATGAGGACAT CCAGCTCAGC 
CCTTTCATGG CTGAAATCAT GCCCGCCCCA GGAAAAGACT TTTATCCTGA CAAGGTGAAG 
GCTTATGGCC CCGGTCTGCA GAGCTCAGGC CTGTCAGTTG GCAAACCAGC CGAGTTTACC 
GTGGATGCCA AACTGGGTGG CAAAGCTCCT CTGAAGATTC AAGCTCAGGA CCGTGATGGA 
AACCCAGTCG ATGTGCAGGT GAAGGACAAT GGAAATGGGA CATACAGCTG CAGCTACACC 
CCTCGCAAAC CAGTCAAACA CACTGTGATG GTGTCCTGGG GCGGCGTCAA CATCCCAGAG 
AGCCCGTTCA GAATGAACAT TGGGGCAGGG TGTCATCCCA ATAAGGTGAA AGTATCTGGA 
CCTGGAGTGG CCAAGACAGG CCTCAAAGCA TATGATCCAA CATACTTTAC TGTGGATTGT 
GCTGAGGCTG GACAGGGCGA CATCAGCATA GGCATCAAAT GTGCTGCAGG TGTGGTGGGA 
CCTGCTGAGG CTGACATAGA CTTCGACATC ATTAGAAATG ACAACGACAC ATTTACTGTT 
AAATACACTC CTCCTGGAGC GGGCAGCTAC ACCATCATGG TGCTGTTTGC TGATCAGACC 
ATTCCCATGA CACCCATCAG AATTAAGGTT GATCCCTCTC ATGATGCAAG CAAGGTTAAA 
GCTGAGGGGC CTGGACTTAG CCGTTCTGGT GTGGAGCTGA ACAAACCCAC TCACTTCACT 
GTGAACACAA AGGCTGCAGG CAAGGCCAAG CTTGATGCTC AGTTCACTGG ACCCAACAAG 
GGAGATGCAG TCCGTGACTT CGACATAATC GACAACCATG ATGGCACTCA CACTGTCAAA 
TATACCCCTG TCCAACAGGG CAACATGGGT TTGAATGTCA CCTACGGTGG TGACCCTATT 
CCCAAGAGCC CATTTGCAGT AGCAATTGCC CCCTCTCTGG ACCTCAGTAA AGTTAAAGTG 
GCTGGTCTTG GAAGTAAAAT GACTGTTGGA AAGGATCAGG AGATCACAGT CAAATCTAAA 
GGTGCAGGCG GTCAGGGCAA AGTTGGCGCA AAGGTTACTG GTCCATCTGC TAAATCAGTG 
CCCTGTAAAG TTGAACCAGG CCTGAGCCCA GAAACCAGCC AAGTTCGTTT TATCCCAAGA 
GATAAGGGAC CCTATGAGGT GGAACTGACA TATGATGGCG CCCCCATCCC TGGCAGTCCA 
TTCCCCATCG AAGCCATTGC ACCTACTGAC CCATCCAAGG TGCGATGCTC TGGGCCAGGA 
CTGGAGCGGG CGAAGGTTGG CGAGCCTGGA CAGTTTGTGG TGGATTGCAC TAATGCTGGC 
CCTGCTGAGT TGACCATCGA GATCATCTCT GACAATGGCA CTGAGGCTGA AGTCCACATT 
CAAGACAACG GCGACGGGAC CTACACCATC ACTTACGTCC CTCTCTACCC TGGAGCCTAT 
ACCCTCACCA TCCGCTACGG GGACCAGGAT GTGCCAAACT TCCCAGCAAG ACTCCATGTG 
GAGCCTGCTG TGGAGACCAG TGGAGTGAAA GTGTTCGGAC CCGGAGTGGA GGGCAAAGGT 
GTTTTCAGGG AAGCCACTAC AGATTTCACT GTGGATGCCC GTGCGCTCAC TAAAACCGGA 
GGCAATCACA TCAAAACCTG CATCAACAAC CCATCGGGCA ACCGCACTGA AGCACTGATC 
CGAGATCTGG GAGACGGCAC ATATCAAGTG GAGTACACGC CTTATGAGGA GGGTACACAC 
AGTGTTGAGG TTGCATATGA CAGCTCCCCG GTTCCCAAAA GCCCCTTCCG TGTGCCAGTG 
ACCGAGGGCT GTGACCCGGC GCGTGTGCGT GTGCATGGTC CAGGACTTCA GTCTGGCATC 
ACCAACAAAC CTAACAAATT TACCGTGGAG ACCCGTGGGG CTGGTACTGG TGGACTGGGG 
CTGGCTATGG AAGGACCTTC TGAGGCCAAA ATGTCCTGCA CCGATAACAA AGATGGCAGC 
TGTTGTGTTG AATACATCCC ATATGAGCCA GGGACATACA ACCTCAATGT CACCTATGGA 
GGCCAACCAA TCACTGGAAG CCCTTTCTCT GTACCTGTCC ATGATACTGT TGACCCAACT 
AAAGTGAAAT GTCAGGGCCA GGGGCTTGGA AACAACGTGC GTGCCAATAT CCCACAGGTC 
TTTTCTGTGG ATGCAAGCAA GGCTGGTATG GCGCCACTGC AGGTCAGAGT TCAGGGACCC 
AAAGGTGTTG TGGAGCCTGC TGAGGTGGTG GATAATGGTG ACCAGACTCA CACAGTCAGC 
TATGTGCCCA CAAGAGAGGG GCCATACTCC ATTAATGTTC TGTATGCTGA TGAGGAGATC 
CCACAGAGTC CTTATAAGGT GAAGGTTCTG CCCACTCATG ACGCAAGTAA AGTCCGTTGC 
AGCGGTCCTG GCCTGAATAC CACTGGTGTT CCTGCCAGTC TGCCCGTGGA GTTCACTATT 
GACGCCAAAG ATGCAGGAGA GGGACTGTTG GCCGTCCAGA TAACTGACCC TGATGGGAAG 
CCAAAGAAGG CTAACATTCG TGATAATCAG GACGGGACCT ACCTTGTGTC CTATGTGCCT 
GACATGACCG GCCGTTACAC CATTCTTATT AAGTATGGTG GTGATGAGAT CCCGTATTCT 
CCATATCGCA TTCGAGCCCT GCCTACTGGA GACGCCAGCA AGTGTACAGT CACAGTCTCA 
ATTGGAGGTC ATGGTTTGGG AGCTGGAGTT GGTCCGACTA TTCAGATTGG AGAGCAGACT 
GTCATCACTG TGGATGCGAA GGCAGCTGGG AAGGGCAAGG TGACCTGTAC CGTGTGCACA 
CCTGATGGAG GCGAGGTGGA TGTGGACGTC GTGGAAAATG AGGATGGAAC GTTTGACATC 
TTTTACACTG CACCGCAGCC TGGGAAATAC GTGATCTGTG TGCGCTTCGG TGGAGAGCAC 
ATCCCTAACA GCCCCTTCCA AGTCACGGCT ACAGATGGGC AATTGGGTAT GAATGGACTG 
GATGTAGCTG GACTGAGACC CTTTGACTTG GTCATTCCTT TCACCATTCA GAAAGGAGAA 
ATTACAGGTG ATGTACGAAT GCCCTCTGGA AAAGTGGCCA AGCCTGATAT TACAGACAAC 
AAGGATGGCA CAGTCACAGT GAAATATGCC CCAACTGAGG CGGGCCTGCA TGAGATGGAC 
ATTAAATATG ATGGAATACA CATTCCAGGA AGTCCATTGC AATTCTACGT GGATTATGTC 
AACAGCGGTC ATGTGACTGC ATATGGTCCT GGTCTAATTC ATGGCATGGT CAATAAGCCT 
GCAGTCTTCA CAGTCAACAC AAAGGATGCT GGAGAGGGTG GTCTGTCTCT GGCCATCGAG 
GGGCCATCAA AGGCAGATAT CAGCTGCACT GATAATCAGG ACGGTACCTG CACTGTTTCA 
TACCTCCCTG TTCTTCCCGG AGACTACAAT ATCATTGTTA AATACAATGA CAAGAACATC 
CCCGGCAGCC CTTTCATGTC CAAAATCACA GGTGACGACT CGATGCGCAT GTCTCACCTG 
AAGGTGGGCT CAGCTGCTGA CATCCCTCTG GACATTGGTG AGCTTGACCT CAGTCAGCTG 
ACAGCCACAC TCACCACCCC ATCCGGCCGA GAGGAGCCTT GCCTGCTCAA GATGCTCAGA 
AATGGGCATG TGGGAATTTC CTTTGTGCCC AAGGAAATTG GCGAGCATCT TGTTAACATT 
AAGAAAAATG GACGCCATAT TCCTAGTAGC CCCATTTCTG TCATGATCAA CCAATCAGAG 
ATCGGTGATG CCAGCCGTGT ACGAGTAACT GGTCAAGGCC TGAGTGAAGC TCGCACCTTT 
GAGCCCGCAG AATTCATCAT TGACACTAGA GATGCAGGAT ATGGTGGACT CAGTTTGTCT 
ATTGAGGGAC CTAGTAAAGT GGATATCAAC ACTGAAGATC AGGAAGATGG CACCTGCAAA 
GTGACATATT GCCCCACAGA GCCTGGAAAC TACATAATTA ACATCAAATT TGCAGACCAG 
CATGTGCCAG GAAGTGCTTT CACTGTGAAA GTGACAGGCG AGGGTCGGAT GAAGGAGAGC 
ATCACCCGCA AAAGAAGAGC TGCTTCTGTG GCCAATGTTG GCAGCCAGTG CGACCTCAGT 
CTAAAGATCC CTGAGATCAG CATTGCAGAC ATGACCGCTC AGGTGACCAG CCCATCTGGC 
AAAGTCCACA AGGCAGAGAT AATGGAGGGA GAAAACAACA CCTACTGCAT CCGATTCGTG 
CCCACAGAGA TGGGTGTGCA CACAGTAAGC GTGAAGTATC AAGGTCAGCA TGTGCCTGGA 
TCCCCCTTCC AGTTCACGGT GGGACCTCTT GGAGAAGGAG GAGCTCATAA AGTCAGAGCT 
GGAGGCCCAG GATTAGAGAG GGCAGAAGCT GGTGTACCAG CGGAGTTCAG CATTTGGACT 
CGTGAAGCAG GAGCTGGTGG TCTGTCCATC GCAGTGGAAG GACCCAGCAA AGCAGAGATT 
GCTTTTGAAG ACCGTAAAGA TGGATCCAGT GGTGTCTCTT ACATCGTCCA GGAGCCTGGC 
GATTATGAAG TATCCATCAA GTTCAATGAC GAGCACATCC CTGACAGTCC GTTTGTGGTG 
CCTGTGGCCT CGCCATCTGA CGATGCCCGC CGTCTCACTG TTGCCAGTCT TCAGGAGTCC 
GGGTTAAAGG TGAACCAGCC GGCCTCTTTT GCTGTGAGCC TAAATGGGGC GAAAGGAGTC 
ATCGATGCCA AAGTTCACAG TCCATCAGGA GCACTTGAAG AGTGCTGCGT CACCGAGATC 
GATGAAGATA AATATGCTGT GAGATTCATT CCCAGGGAGA ATGGATTGTA CCTGATAGAT 
GTGAAATTTA ATGGTTCTCA CATCCCTGGA AGCCCCTTCA AGATCCGTGT TGGTGAGACG 
GGTCAGGCTG GAGATCCAGG AATGGTGTCT GCTTATGGAG CTGGTCTGGA AGGAGGCACT 
ACTGGATCGC CTTGTGAGTT CATTGTAAAC ACCAGTTCAG CTGGCCCTGG AGCTTTGGCG 
GTGACTATCG ACGGGCCGTC CAAGGTGAAG ATGGACTGTC AGGAATGCCC AGAGGGCTAC 
AAAGTCACCT ACACACCTAT GGCCCCTGGA AACTACCTAA TTTCTATTAA ATACGGTGGA 
CCGTACCATA TTGTTGGTAG TCCATTCAAA GCTAAAATTA CTGGCTCCCG ATTGGTCAAC 
AGCCACAGCA TGCATGAGAC TTCATCAGTA CTGGTTGACC CTGTGACCCG CAGCATGACG 
ACCAGCCAGC AGGCAGCGCC AGGCTGGGCC AGCTCTGATG CCAGCCGCGT AGTGGCCAAA 
GGTTTGGGGC TCAACAAGGG CTTCATAGGA CAGAAGAACA CTTTCAGTGT GGACTGTAGT 
AAAGCAGGGA GGAACATGCT CCTGGTCGGA GTGGATGGAC CCAAAGTGCC CTGTGAGGAG 
ATCCTGGTGA AACACCTGGG CAACCGCCTC TACAACGTTT CCTACCAGCT GAAGGAGAAA 
GGAGAGTACA TTCTGGTGGT CAAATGGGGT GATGAGCACA TCCCGGGCAG CCCGTATCAC 
ATCACTGTCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

flna ( XM_009296770.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295045.1 Danio rerio filamin A, alpha (actin binding protein 280) (flna), transcript variant X1, mRNA.

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CloneID	ODa43841
Clone ID Related Accession (Same CDS sequence)	XM_009296770.2 , XM_021474523.1 , XM_009296770.3
Accession Version	XM_009296770.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	filamin-A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296770.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCAAC ACACACGCCC CCACCAGTCG AGCGCCGCCG CTCCCCTATC CAACGCTTCT 
CTCTCCCCGG ACAAGGACGC CGACATGCCC GCTACAGAGA AAGATCTCGC CGAGGACGCG 
CCATGGAAGA AAATCCAGCA AAATACATTC ACGCGTTGGT GCAACGAGCA TCTGAAATGT 
GTCAACAAGC GCATAGCGAA CCTGCAGACG GACCTGAGCG ACGGGCTCCG GCTGATCGCG 
CTTCTGGAGG TGCTCAGCCA GAAGAAGATG TTCCGCAAGT ACAACCAGCG CCCTACCTTC 
AGACAGATGC AGCTGGAGAA CGTCTCCGTG GCGCTGGAGT TCCTGGACAT TGAAAACATC 
AAGCTGGTCT CCATTGACAG TAAGGCCATA GTGGATGGTA ACCTGAAGCT GATCCTGGGT 
CTGATATGGA CTCTTATTCT GCATTATTCC ATCTCCATGC CCATGTGGGA CGAGGAAGAG 
GAGAGCGAGG AAAGCAAGCA GAAGACGCCC AAGCAGAGGC TCCTCGGATG GATCCAGAAC 
AAGCTGCCCG AGCTGCCAAT CACCAACTTC AGCCGCGATT GGCAGTCAGG GAAAGCCCTT 
GGCGCTCTGG TGGACAGTTG TGCTCCAGGT CTGTGTCCAG ACTGGGATTC CTGGGACCAG 
ACCAAGCCTG TGGACAATGC CAGAGAGGCC ATGCAGCAGG CTGATGACTG GCTCGGTGTT 
CCTCAGGTCA TCACACCAGA AGAAATCGTT GATCCCAATG TGGACGAGCA CTCCGTTATG 
ACTTACCTGT CCCAGTTTCC CAAAGCCAAA CTCAAGCCTG GTGCCCCTCT GAGGCCCAAA 
CTCAATCCCA AAAAGGCCCG TGCTTATGGA CCAGGTATTG AGCCAACAGG TAATGTTGTG 
ATGAAGAAGG CTGTGTTCAC TGTTGAGACC ATCAGCGCTG GTCAGGGTGA GGTACTGGTT 
TATGTCGAGG ACCCCGCTGG ACACCGCGAG GAGGCCAAAG TCACAGCCAA CAATGACAAG 
AACCGCACCT ACTCAGTATT CTATGTGCCA AAAGTCACTG GACAACACAA GGTGACAGTG 
CTGTTTGCAG GGCAACACAT CTCTAAGAGC CCGTTTGAGG TGGATGTGGG AATGGCTCAG 
GGAGACTCCA GCAAGGTCAC TGCCCAGGGC CCAGGACTCG AGCCTGCAGG CAACATTGCC 
AACAAAACCA CATACTTTGA TGTCTACACA GCTGGTGCTG GAGTAGGAGA GGTGGAGGTG 
GTCATCATGG ACCCCAGTGG AAAGAAGGAC ACGGTTGAGT GCAGTGTCGA GGATAAAGGC 
AACAGCAGCT ACCGATGCAC CTACAAGCCC ACTCAGGAGG GACAGCACAT CATTTACATC 
ACCTTTGCCG GGGGACAGAT CTCCAAGAGC CCTTACACTG TCAATGTGGG AGAGGCCTGT 
AATCCAAGCC TGTGTACAGC CAAGGGCCGC GGTCTGCAGC CTAAAGGCCT GAGGGTCAAG 
GAGACCGCCG AGTTTAAGGT TTACACCAAA GGAGCAGGAA CCGGAGACCT CAAAGTTACC 
ATCAAAGGGC CCAAGGGTCT CGAGGAGCCC TGTAAGAAGA AGGATTTGGG AGACGGCGTG 
TACAGCTTTG ATTATTACCC CTCCACACCT GGAAATTACA TCATCACCAT CACATGGGGC 
GGTCAACACA TCCCACGCAG CCCGTTTGAG GTGAAGATCG GGTCTGAAGC AGGACCGCAG 
CAGGTCAGGG CGTGGGGTCC AGGACTAGAG AGTGGCGTTG TGGGCAAATC TGCTGACTTT 
GTTGTGGAAG CTGTTGGTGA TGATGTGGGC ACTTTAGGTT TCTCTGTTGA AGGCCCTTCC 
CCGGCTAAGA TTGAATGTGA TGATAAGGGA GATGGTTCAT GTGACGTGAG GTACTGGCCC 
ACCGAACCGG GTGAATATGC AGTTCATGTG CTCTGTAAGA ATGAGGACAT CCAGCTCAGC 
CCTTTCATGG CTGAAATCAT GCCCGCCCCA GGAAAAGACT TTTATCCTGA CAAGGTGAAG 
GCTTATGGCC CCGGTCTGCA GAGCTCAGGC CTGTCAGTTG GCAAACCAGC CGAGTTTACC 
GTGGATGCCA AACTGGGTGG CAAAGCTCCT CTGAAGATTC AAGCTCAGGA CCGTGATGGA 
AACCCAGTCG ATGTGCAGGT GAAGGACAAT GGAAATGGGA CATACAGCTG CAGCTACACC 
CCTCGCAAAC CAGTCAAACA CACTGTGATG GTGTCCTGGG GCGGCGTCAA CATCCCAGAG 
AGCCCGTTCA GAATGAACAT TGGGGCAGGG TGTCATCCCA ATAAGGTGAA AGTATCTGGA 
CCTGGAGTGG CCAAGACAGG CCTCAAAGCA TATGATCCAA CATACTTTAC TGTGGATTGT 
GCTGAGGCTG GACAGGGCGA CATCAGCATA GGCATCAAAT GTGCTGCAGG TGTGGTGGGA 
CCTGCTGAGG CTGACATAGA CTTCGACATC ATTAGAAATG ACAACGACAC ATTTACTGTT 
AAATACACTC CTCCTGGAGC GGGCAGCTAC ACCATCATGG TGCTGTTTGC TGATCAGACC 
ATTCCCATGA CACCCATCAG AATTAAGGTT GATCCCTCTC ATGATGCAAG CAAGGTTAAA 
GCTGAGGGGC CTGGACTTAG CCGTTCTGGT GTGGAGCTGA ACAAACCCAC TCACTTCACT 
GTGAACACAA AGGCTGCAGG CAAGGCCAAG CTTGATGCTC AGTTCACTGG ACCCAACAAG 
GGAGATGCAG TCCGTGACTT CGACATAATC GACAACCATG ATGGCACTCA CACTGTCAAA 
TATACCCCTG TCCAACAGGG CAACATGGGT TTGAATGTCA CCTACGGTGG TGACCCTATT 
CCCAAGAGCC CATTTGCAGT AGCAATTGCC CCCTCTCTGG ACCTCAGTAA AGTTAAAGTG 
GCTGGTCTTG GAAGTAAAAT GACTGTTGGA AAGGATCAGG AGATCACAGT CAAATCTAAA 
GGTGCAGGCG GTCAGGGCAA AGTTGGCGCA AAGGTTACTG GTCCATCTGC TAAATCAGTG 
CCCTGTAAAG TTGAACCAGG CCTGAGCCCA GAAACCAGCC AAGTTCGTTT TATCCCAAGA 
GATAAGGGAC CCTATGAGGT GGAACTGACA TATGATGGCG CCCCCATCCC TGGCAGTCCA 
TTCCCCATCG AAGCCATTGC ACCTACTGAC CCATCCAAGG TGCGATGCTC TGGGCCAGGA 
CTGGAGCGGG CGAAGGTTGG CGAGCCTGGA CAGTTTGTGG TGGATTGCAC TAATGCTGGC 
CCTGCTGAGT TGACCATCGA GATCATCTCT GACAATGGCA CTGAGGCTGA AGTCCACATT 
CAAGACAACG GCGACGGGAC CTACACCATC ACTTACGTCC CTCTCTACCC TGGAGCCTAT 
ACCCTCACCA TCCGCTACGG GGACCAGGAT GTGCCAAACT TCCCAGCAAG ACTCCATGTG 
GAGCCTGCTG TGGAGACCAG TGGAGTGAAA GTGTTCGGAC CCGGAGTGGA GGGCAAAGGT 
GTTTTCAGGG AAGCCACTAC AGATTTCACT GTGGATGCCC GTGCGCTCAC TAAAACCGGA 
GGCAATCACA TCAAAACCTG CATCAACAAC CCATCGGGCA ACCGCACTGA AGCACTGATC 
CGAGATCTGG GAGACGGCAC ATATCAAGTG GAGTACACGC CTTATGAGGA GGGTACACAC 
AGTGTTGAGG TTGCATATGA CAGCTCCCCG GTTCCCAAAA GCCCCTTCCG TGTGCCAGTG 
ACCGAGGGCT GTGACCCGGC GCGTGTGCGT GTGCATGGTC CAGGACTTCA GTCTGGCATC 
ACCAACAAAC CTAACAAATT TACCGTGGAG ACCCGTGGGG CTGGTACTGG TGGACTGGGG 
CTGGCTATGG AAGGACCTTC TGAGGCCAAA ATGTCCTGCA CCGATAACAA AGATGGCAGC 
TGTTGTGTTG AATACATCCC ATATGAGCCA GGGACATACA ACCTCAATGT CACCTATGGA 
GGCCAACCAA TCACTGGAAG CCCTTTCTCT GTACCTGTCC ATGATACTGT TGACCCAACT 
AAAGTGAAAT GTCAGGGCCA GGGGCTTGGA AACAACGTGC GTGCCAATAT CCCACAGGTC 
TTTTCTGTGG ATGCAAGCAA GGCTGGTATG GCGCCACTGC AGGTCAGAGT TCAGGGACCC 
AAAGGTGTTG TGGAGCCTGC TGAGGTGGTG GATAATGGTG ACCAGACTCA CACAGTCAGC 
TATGTGCCCA CAAGAGAGGG GCCATACTCC ATTAATGTTC TGTATGCTGA TGAGGAGATC 
CCACAGAGTC CTTATAAGGT GAAGGTTCTG CCCACTCATG ACGCAAGTAA AGTCCGTTGC 
AGCGGTCCTG GCCTGAATAC CACTGGTGTT CCTGCCAGTC TGCCCGTGGA GTTCACTATT 
GACGCCAAAG ATGCAGGAGA GGGACTGTTG GCCGTCCAGA TAACTGACCC TGATGGGAAG 
CCAAAGAAGG CTAACATTCG TGATAATCAG GACGGGACCT ACCTTGTGTC CTATGTGCCT 
GACATGACCG GCCGTTACAC CATTCTTATT AAGTATGGTG GTGATGAGAT CCCGTATTCT 
CCATATCGCA TTCGAGCCCT GCCTACTGGA GACGCCAGCA AGTGTACAGT CACAGTCTCA 
ATTGGAGGTC ATGGTTTGGG AGCTGGAGTT GGTCCGACTA TTCAGATTGG AGAGCAGACT 
GTCATCACTG TGGATGCGAA GGCAGCTGGG AAGGGCAAGG TGACCTGTAC CGTGTGCACA 
CCTGATGGAG GCGAGGTGGA TGTGGACGTC GTGGAAAATG AGGATGGAAC GTTTGACATC 
TTTTACACTG CACCGCAGCC TGGGAAATAC GTGATCTGTG TGCGCTTCGG TGGAGAGCAC 
ATCCCTAACA GCCCCTTCCA AGTCACGGCT TTGGAAGGTG ACTCTCCTGA CCAACTAATG 
CAGCAGAGCC AGAACCTGCA GTACGCCTAC GCGCCCAACA TGGGCCAGAC ATGGGCTACA 
GATGGGCAAT TGGGTATGAA TGGACTGGAT GTAGCTGGAC TGAGACCCTT TGACTTGGTC 
ATTCCTTTCA CCATTCAGAA AGGAGAAATT ACAGGTGATG TACGAATGCC CTCTGGAAAA 
GTGGCCAAGC CTGATATTAC AGACAACAAG GATGGCACAG TCACAGTGAA ATATGCCCCA 
ACTGAGGCGG GCCTGCATGA GATGGACATT AAATATGATG GAATACACAT TCCAGGAAGT 
CCATTGCAAT TCTACGTGGA TTATGTCAAC AGCGGTCATG TGACTGCATA TGGTCCTGGT 
CTAATTCATG GCATGGTCAA TAAGCCTGCA GTCTTCACAG TCAACACAAA GGATGCTGGA 
GAGGGTGGTC TGTCTCTGGC CATCGAGGGG CCATCAAAGG CAGATATCAG CTGCACTGAT 
AATCAGGACG GTACCTGCAC TGTTTCATAC CTCCCTGTTC TTCCCGGAGA CTACAATATC 
ATTGTTAAAT ACAATGACAA GAACATCCCC GGCAGCCCTT TCATGTCCAA AATCACAGGT 
GACGACTCGA TGCGCATGTC TCACCTGAAG GTGGGCTCAG CTGCTGACAT CCCTCTGGAC 
ATTGGTGAGC TTGACCTCAG TCAGCTGACA GCCACACTCA CCACCCCATC CGGCCGAGAG 
GAGCCTTGCC TGCTCAAGAT GCTCAGAAAT GGGCATGTGG GAATTTCCTT TGTGCCCAAG 
GAAATTGGCG AGCATCTTGT TAACATTAAG AAAAATGGAC GCCATATTCC TAGTAGCCCC 
ATTTCTGTCA TGATCAACCA ATCAGAGATC GGTGATGCCA GCCGTGTACG AGTAACTGGT 
CAAGGCCTGA GTGAAGCTCG CACCTTTGAG CCCGCAGAAT TCATCATTGA CACTAGAGAT 
GCAGGATATG GTGGACTCAG TTTGTCTATT GAGGGACCTA GTAAAGTGGA TATCAACACT 
GAAGATCAGG AAGATGGCAC CTGCAAAGTG ACATATTGCC CCACAGAGCC TGGAAACTAC 
ATAATTAACA TCAAATTTGC AGACCAGCAT GTGCCAGGAA GTGCTTTCAC TGTGAAAGTG 
ACAGGCGAGG GTCGGATGAA GGAGAGCATC ACCCGCAAAA GAAGAGCTGC TTCTGTGGCC 
AATGTTGGCA GCCAGTGCGA CCTCAGTCTA AAGATCCCTG AGATCAGCAT TGCAGACATG 
ACCGCTCAGG TGACCAGCCC ATCTGGCAAA GTCCACAAGG CAGAGATAAT GGAGGGAGAA 
AACAACACCT ACTGCATCCG ATTCGTGCCC ACAGAGATGG GTGTGCACAC AGTAAGCGTG 
AAGTATCAAG GTCAGCATGT GCCTGGATCC CCCTTCCAGT TCACGGTGGG ACCTCTTGGA 
GAAGGAGGAG CTCATAAAGT CAGAGCTGGA GGCCCAGGAT TAGAGAGGGC AGAAGCTGGT 
GTACCAGCGG AGTTCAGCAT TTGGACTCGT GAAGCAGGAG CTGGTGGTCT GTCCATCGCA 
GTGGAAGGAC CCAGCAAAGC AGAGATTGCT TTTGAAGACC GTAAAGATGG ATCCAGTGGT 
GTCTCTTACA TCGTCCAGGA GCCTGGCGAT TATGAAGTAT CCATCAAGTT CAATGACGAG 
CACATCCCTG ACAGTCCGTT TGTGGTGCCT GTGGCCTCGC CATCTGACGA TGCCCGCCGT 
CTCACTGTTG CCAGTCTTCA GGAGTCCGGG TTAAAGGTGA ACCAGCCGGC CTCTTTTGCT 
GTGAGCCTAA ATGGGGCGAA AGGAGTCATC GATGCCAAAG TTCACAGTCC ATCAGGAGCA 
CTTGAAGAGT GCTGCGTCAC CGAGATCGAT GAAGATAAAT ATGCTGTGAG ATTCATTCCC 
AGGGAGAATG GATTGTACCT GATAGATGTG AAATTTAATG GTTCTCACAT CCCTGGAAGC 
CCCTTCAAGA TCCGTGTTGG TGAGACGGGT CAGGCTGGAG ATCCAGGAAT GGTGTCTGCT 
TATGGAGCTG GTCTGGAAGG AGGCACTACT GGATCGCCTT GTGAGTTCAT TGTAAACACC 
AGTTCAGCTG GCCCTGGAGC TTTGGCGGTG ACTATCGACG GGCCGTCCAA GGTGAAGATG 
GACTGTCAGG AATGCCCAGA GGGCTACAAA GTCACCTACA CACCTATGGC CCCTGGAAAC 
TACCTAATTT CTATTAAATA CGGTGGACCG TACCATATTG TTGGTAGTCC ATTCAAAGCT 
AAAATTACTG GCTCCCGATT GGTCAACAGC CACAGCATGC ATGAGACTTC ATCAGTACTG 
GTTGACCCTG TGACCCGCAG CATGACGACC AGCCAGCAGG CAGCGCCAGG CTGGGCCAGC 
TCTGATGCCA GCCGCGTAGT GGCCAAAGGT TTGGGGCTCA ACAAGGGCTT CATAGGACAG 
AAGAACACTT TCAGTGTGGA CTGTAGTAAA GCAGGGAGGA ACATGCTCCT GGTCGGAGTG 
GATGGACCCA AAGTGCCCTG TGAGGAGATC CTGGTGAAAC ACCTGGGCAA CCGCCTCTAC 
AACGTTTCCT ACCAGCTGAA GGAGAAAGGA GAGTACATTC TGGTGGTCAA ATGGGGTGAT 
GAGCACATCC CGGGCAGCCC GTATCACATC ACTGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295045.1
CDS	370..8028
Translation	MSQHTRPHQSSAAAPLSNASLSPDKDADMPATEKDLAEDAPWKKIQQNTFTRWCNEHLKCVNKRIANLQTDLSDGLRLIALLEVLSQKKMFRKYNQRPTFRQMQLENVSVALEFLDIENIKLVSIDSKAIVDGNLKLILGLIWTLILHYSISMPMWDEEEESEESKQKTPKQRLLGWIQNKLPELPITNFSRDWQSGKALGALVDSCAPGLCPDWDSWDQTKPVDNAREAMQQADDWLGVPQVITPEEIVDPNVDEHSVMTYLSQFPKAKLKPGAPLRPKLNPKKARAYGPGIEPTGNVVMKKAVFTVETISAGQGEVLVYVEDPAGHREEAKVTANNDKNRTYSVFYVPKVTGQHKVTVLFAGQHISKSPFEVDVGMAQGDSSKVTAQGPGLEPAGNIANKTTYFDVYTAGAGVGEVEVVIMDPSGKKDTVECSVEDKGNSSYRCTYKPTQEGQHIIYITFAGGQISKSPYTVNVGEACNPSLCTAKGRGLQPKGLRVKETAEFKVYTKGAGTGDLKVTIKGPKGLEEPCKKKDLGDGVYSFDYYPSTPGNYIITITWGGQHIPRSPFEVKIGSEAGPQQVRAWGPGLESGVVGKSADFVVEAVGDDVGTLGFSVEGPSPAKIECDDKGDGSCDVRYWPTEPGEYAVHVLCKNEDIQLSPFMAEIMPAPGKDFYPDKVKAYGPGLQSSGLSVGKPAEFTVDAKLGGKAPLKIQAQDRDGNPVDVQVKDNGNGTYSCSYTPRKPVKHTVMVSWGGVNIPESPFRMNIGAGCHPNKVKVSGPGVAKTGLKAYDPTYFTVDCAEAGQGDISIGIKCAAGVVGPAEADIDFDIIRNDNDTFTVKYTPPGAGSYTIMVLFADQTIPMTPIRIKVDPSHDASKVKAEGPGLSRSGVELNKPTHFTVNTKAAGKAKLDAQFTGPNKGDAVRDFDIIDNHDGTHTVKYTPVQQGNMGLNVTYGGDPIPKSPFAVAIAPSLDLSKVKVAGLGSKMTVGKDQEITVKSKGAGGQGKVGAKVTGPSAKSVPCKVEPGLSPETSQVRFIPRDKGPYEVELTYDGAPIPGSPFPIEAIAPTDPSKVRCSGPGLERAKVGEPGQFVVDCTNAGPAELTIEIISDNGTEAEVHIQDNGDGTYTITYVPLYPGAYTLTIRYGDQDVPNFPARLHVEPAVETSGVKVFGPGVEGKGVFREATTDFTVDARALTKTGGNHIKTCINNPSGNRTEALIRDLGDGTYQVEYTPYEEGTHSVEVAYDSSPVPKSPFRVPVTEGCDPARVRVHGPGLQSGITNKPNKFTVETRGAGTGGLGLAMEGPSEAKMSCTDNKDGSCCVEYIPYEPGTYNLNVTYGGQPITGSPFSVPVHDTVDPTKVKCQGQGLGNNVRANIPQVFSVDASKAGMAPLQVRVQGPKGVVEPAEVVDNGDQTHTVSYVPTREGPYSINVLYADEEIPQSPYKVKVLPTHDASKVRCSGPGLNTTGVPASLPVEFTIDAKDAGEGLLAVQITDPDGKPKKANIRDNQDGTYLVSYVPDMTGRYTILIKYGGDEIPYSPYRIRALPTGDASKCTVTVSIGGHGLGAGVGPTIQIGEQTVITVDAKAAGKGKVTCTVCTPDGGEVDVDVVENEDGTFDIFYTAPQPGKYVICVRFGGEHIPNSPFQVTALEGDSPDQLMQQSQNLQYAYAPNMGQTWATDGQLGMNGLDVAGLRPFDLVIPFTIQKGEITGDVRMPSGKVAKPDITDNKDGTVTVKYAPTEAGLHEMDIKYDGIHIPGSPLQFYVDYVNSGHVTAYGPGLIHGMVNKPAVFTVNTKDAGEGGLSLAIEGPSKADISCTDNQDGTCTVSYLPVLPGDYNIIVKYNDKNIPGSPFMSKITGDDSMRMSHLKVGSAADIPLDIGELDLSQLTATLTTPSGREEPCLLKMLRNGHVGISFVPKEIGEHLVNIKKNGRHIPSSPISVMINQSEIGDASRVRVTGQGLSEARTFEPAEFIIDTRDAGYGGLSLSIEGPSKVDINTEDQEDGTCKVTYCPTEPGNYIINIKFADQHVPGSAFTVKVTGEGRMKESITRKRRAASVANVGSQCDLSLKIPEISIADMTAQVTSPSGKVHKAEIMEGENNTYCIRFVPTEMGVHTVSVKYQGQHVPGSPFQFTVGPLGEGGAHKVRAGGPGLERAEAGVPAEFSIWTREAGAGGLSIAVEGPSKAEIAFEDRKDGSSGVSYIVQEPGDYEVSIKFNDEHIPDSPFVVPVASPSDDARRLTVASLQESGLKVNQPASFAVSLNGAKGVIDAKVHSPSGALEECCVTEIDEDKYAVRFIPRENGLYLIDVKFNGSHIPGSPFKIRVGETGQAGDPGMVSAYGAGLEGGTTGSPCEFIVNTSSAGPGALAVTIDGPSKVKMDCQECPEGYKVTYTPMAPGNYLISIKYGGPYHIVGSPFKAKITGSRLVNSHSMHETSSVLVDPVTRSMTTSQQAAPGWASSDASRVVAKGLGLNKGFIGQKNTFSVDCSKAGRNMLLVGVDGPKVPCEEILVKHLGNRLYNVSYQLKEKGEYILVVKWGDEHIPGSPYHITV

Target ORF information:

RefSeq Version	XM_009296770.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio filamin A, alpha (actin binding protein 280) (flna), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296770.2

ORF Insert Sequence:

ATGAGTCAAC ACACACGCCC CCACCAGTCG AGCGCCGCCG CTCCCCTATC CAACGCTTCT 
CTCTCCCCGG ACAAGGACGC CGACATGCCC GCTACAGAGA AAGATCTCGC CGAGGACGCG 
CCATGGAAGA AAATCCAGCA AAATACATTC ACGCGTTGGT GCAACGAGCA TCTGAAATGT 
GTCAACAAGC GCATAGCGAA CCTGCAGACG GACCTGAGCG ACGGGCTCCG GCTGATCGCG 
CTTCTGGAGG TGCTCAGCCA GAAGAAGATG TTCCGCAAGT ACAACCAGCG CCCTACCTTC 
AGACAGATGC AGCTGGAGAA CGTCTCCGTG GCGCTGGAGT TCCTGGACAT TGAAAACATC 
AAGCTGGTCT CCATTGACAG TAAGGCCATA GTGGATGGTA ACCTGAAGCT GATCCTGGGT 
CTGATATGGA CTCTTATTCT GCATTATTCC ATCTCCATGC CCATGTGGGA CGAGGAAGAG 
GAGAGCGAGG AAAGCAAGCA GAAGACGCCC AAGCAGAGGC TCCTCGGATG GATCCAGAAC 
AAGCTGCCCG AGCTGCCAAT CACCAACTTC AGCCGCGATT GGCAGTCAGG GAAAGCCCTT 
GGCGCTCTGG TGGACAGTTG TGCTCCAGGT CTGTGTCCAG ACTGGGATTC CTGGGACCAG 
ACCAAGCCTG TGGACAATGC CAGAGAGGCC ATGCAGCAGG CTGATGACTG GCTCGGTGTT 
CCTCAGGTCA TCACACCAGA AGAAATCGTT GATCCCAATG TGGACGAGCA CTCCGTTATG 
ACTTACCTGT CCCAGTTTCC CAAAGCCAAA CTCAAGCCTG GTGCCCCTCT GAGGCCCAAA 
CTCAATCCCA AAAAGGCCCG TGCTTATGGA CCAGGTATTG AGCCAACAGG TAATGTTGTG 
ATGAAGAAGG CTGTGTTCAC TGTTGAGACC ATCAGCGCTG GTCAGGGTGA GGTACTGGTT 
TATGTCGAGG ACCCCGCTGG ACACCGCGAG GAGGCCAAAG TCACAGCCAA CAATGACAAG 
AACCGCACCT ACTCAGTATT CTATGTGCCA AAAGTCACTG GACAACACAA GGTGACAGTG 
CTGTTTGCAG GGCAACACAT CTCTAAGAGC CCGTTTGAGG TGGATGTGGG AATGGCTCAG 
GGAGACTCCA GCAAGGTCAC TGCCCAGGGC CCAGGACTCG AGCCTGCAGG CAACATTGCC 
AACAAAACCA CATACTTTGA TGTCTACACA GCTGGTGCTG GAGTAGGAGA GGTGGAGGTG 
GTCATCATGG ACCCCAGTGG AAAGAAGGAC ACGGTTGAGT GCAGTGTCGA GGATAAAGGC 
AACAGCAGCT ACCGATGCAC CTACAAGCCC ACTCAGGAGG GACAGCACAT CATTTACATC 
ACCTTTGCCG GGGGACAGAT CTCCAAGAGC CCTTACACTG TCAATGTGGG AGAGGCCTGT 
AATCCAAGCC TGTGTACAGC CAAGGGCCGC GGTCTGCAGC CTAAAGGCCT GAGGGTCAAG 
GAGACCGCCG AGTTTAAGGT TTACACCAAA GGAGCAGGAA CCGGAGACCT CAAAGTTACC 
ATCAAAGGGC CCAAGGGTCT CGAGGAGCCC TGTAAGAAGA AGGATTTGGG AGACGGCGTG 
TACAGCTTTG ATTATTACCC CTCCACACCT GGAAATTACA TCATCACCAT CACATGGGGC 
GGTCAACACA TCCCACGCAG CCCGTTTGAG GTGAAGATCG GGTCTGAAGC AGGACCGCAG 
CAGGTCAGGG CGTGGGGTCC AGGACTAGAG AGTGGCGTTG TGGGCAAATC TGCTGACTTT 
GTTGTGGAAG CTGTTGGTGA TGATGTGGGC ACTTTAGGTT TCTCTGTTGA AGGCCCTTCC 
CCGGCTAAGA TTGAATGTGA TGATAAGGGA GATGGTTCAT GTGACGTGAG GTACTGGCCC 
ACCGAACCGG GTGAATATGC AGTTCATGTG CTCTGTAAGA ATGAGGACAT CCAGCTCAGC 
CCTTTCATGG CTGAAATCAT GCCCGCCCCA GGAAAAGACT TTTATCCTGA CAAGGTGAAG 
GCTTATGGCC CCGGTCTGCA GAGCTCAGGC CTGTCAGTTG GCAAACCAGC CGAGTTTACC 
GTGGATGCCA AACTGGGTGG CAAAGCTCCT CTGAAGATTC AAGCTCAGGA CCGTGATGGA 
AACCCAGTCG ATGTGCAGGT GAAGGACAAT GGAAATGGGA CATACAGCTG CAGCTACACC 
CCTCGCAAAC CAGTCAAACA CACTGTGATG GTGTCCTGGG GCGGCGTCAA CATCCCAGAG 
AGCCCGTTCA GAATGAACAT TGGGGCAGGG TGTCATCCCA ATAAGGTGAA AGTATCTGGA 
CCTGGAGTGG CCAAGACAGG CCTCAAAGCA TATGATCCAA CATACTTTAC TGTGGATTGT 
GCTGAGGCTG GACAGGGCGA CATCAGCATA GGCATCAAAT GTGCTGCAGG TGTGGTGGGA 
CCTGCTGAGG CTGACATAGA CTTCGACATC ATTAGAAATG ACAACGACAC ATTTACTGTT 
AAATACACTC CTCCTGGAGC GGGCAGCTAC ACCATCATGG TGCTGTTTGC TGATCAGACC 
ATTCCCATGA CACCCATCAG AATTAAGGTT GATCCCTCTC ATGATGCAAG CAAGGTTAAA 
GCTGAGGGGC CTGGACTTAG CCGTTCTGGT GTGGAGCTGA ACAAACCCAC TCACTTCACT 
GTGAACACAA AGGCTGCAGG CAAGGCCAAG CTTGATGCTC AGTTCACTGG ACCCAACAAG 
GGAGATGCAG TCCGTGACTT CGACATAATC GACAACCATG ATGGCACTCA CACTGTCAAA 
TATACCCCTG TCCAACAGGG CAACATGGGT TTGAATGTCA CCTACGGTGG TGACCCTATT 
CCCAAGAGCC CATTTGCAGT AGCAATTGCC CCCTCTCTGG ACCTCAGTAA AGTTAAAGTG 
GCTGGTCTTG GAAGTAAAAT GACTGTTGGA AAGGATCAGG AGATCACAGT CAAATCTAAA 
GGTGCAGGCG GTCAGGGCAA AGTTGGCGCA AAGGTTACTG GTCCATCTGC TAAATCAGTG 
CCCTGTAAAG TTGAACCAGG CCTGAGCCCA GAAACCAGCC AAGTTCGTTT TATCCCAAGA 
GATAAGGGAC CCTATGAGGT GGAACTGACA TATGATGGCG CCCCCATCCC TGGCAGTCCA 
TTCCCCATCG AAGCCATTGC ACCTACTGAC CCATCCAAGG TGCGATGCTC TGGGCCAGGA 
CTGGAGCGGG CGAAGGTTGG CGAGCCTGGA CAGTTTGTGG TGGATTGCAC TAATGCTGGC 
CCTGCTGAGT TGACCATCGA GATCATCTCT GACAATGGCA CTGAGGCTGA AGTCCACATT 
CAAGACAACG GCGACGGGAC CTACACCATC ACTTACGTCC CTCTCTACCC TGGAGCCTAT 
ACCCTCACCA TCCGCTACGG GGACCAGGAT GTGCCAAACT TCCCAGCAAG ACTCCATGTG 
GAGCCTGCTG TGGAGACCAG TGGAGTGAAA GTGTTCGGAC CCGGAGTGGA GGGCAAAGGT 
GTTTTCAGGG AAGCCACTAC AGATTTCACT GTGGATGCCC GTGCGCTCAC TAAAACCGGA 
GGCAATCACA TCAAAACCTG CATCAACAAC CCATCGGGCA ACCGCACTGA AGCACTGATC 
CGAGATCTGG GAGACGGCAC ATATCAAGTG GAGTACACGC CTTATGAGGA GGGTACACAC 
AGTGTTGAGG TTGCATATGA CAGCTCCCCG GTTCCCAAAA GCCCCTTCCG TGTGCCAGTG 
ACCGAGGGCT GTGACCCGGC GCGTGTGCGT GTGCATGGTC CAGGACTTCA GTCTGGCATC 
ACCAACAAAC CTAACAAATT TACCGTGGAG ACCCGTGGGG CTGGTACTGG TGGACTGGGG 
CTGGCTATGG AAGGACCTTC TGAGGCCAAA ATGTCCTGCA CCGATAACAA AGATGGCAGC 
TGTTGTGTTG AATACATCCC ATATGAGCCA GGGACATACA ACCTCAATGT CACCTATGGA 
GGCCAACCAA TCACTGGAAG CCCTTTCTCT GTACCTGTCC ATGATACTGT TGACCCAACT 
AAAGTGAAAT GTCAGGGCCA GGGGCTTGGA AACAACGTGC GTGCCAATAT CCCACAGGTC 
TTTTCTGTGG ATGCAAGCAA GGCTGGTATG GCGCCACTGC AGGTCAGAGT TCAGGGACCC 
AAAGGTGTTG TGGAGCCTGC TGAGGTGGTG GATAATGGTG ACCAGACTCA CACAGTCAGC 
TATGTGCCCA CAAGAGAGGG GCCATACTCC ATTAATGTTC TGTATGCTGA TGAGGAGATC 
CCACAGAGTC CTTATAAGGT GAAGGTTCTG CCCACTCATG ACGCAAGTAA AGTCCGTTGC 
AGCGGTCCTG GCCTGAATAC CACTGGTGTT CCTGCCAGTC TGCCCGTGGA GTTCACTATT 
GACGCCAAAG ATGCAGGAGA GGGACTGTTG GCCGTCCAGA TAACTGACCC TGATGGGAAG 
CCAAAGAAGG CTAACATTCG TGATAATCAG GACGGGACCT ACCTTGTGTC CTATGTGCCT 
GACATGACCG GCCGTTACAC CATTCTTATT AAGTATGGTG GTGATGAGAT CCCGTATTCT 
CCATATCGCA TTCGAGCCCT GCCTACTGGA GACGCCAGCA AGTGTACAGT CACAGTCTCA 
ATTGGAGGTC ATGGTTTGGG AGCTGGAGTT GGTCCGACTA TTCAGATTGG AGAGCAGACT 
GTCATCACTG TGGATGCGAA GGCAGCTGGG AAGGGCAAGG TGACCTGTAC CGTGTGCACA 
CCTGATGGAG GCGAGGTGGA TGTGGACGTC GTGGAAAATG AGGATGGAAC GTTTGACATC 
TTTTACACTG CACCGCAGCC TGGGAAATAC GTGATCTGTG TGCGCTTCGG TGGAGAGCAC 
ATCCCTAACA GCCCCTTCCA AGTCACGGCT TTGGAAGGTG ACTCTCCTGA CCAACTAATG 
CAGCAGAGCC AGAACCTGCA GTACGCCTAC GCGCCCAACA TGGGCCAGAC ATGGGCTACA 
GATGGGCAAT TGGGTATGAA TGGACTGGAT GTAGCTGGAC TGAGACCCTT TGACTTGGTC 
ATTCCTTTCA CCATTCAGAA AGGAGAAATT ACAGGTGATG TACGAATGCC CTCTGGAAAA 
GTGGCCAAGC CTGATATTAC AGACAACAAG GATGGCACAG TCACAGTGAA ATATGCCCCA 
ACTGAGGCGG GCCTGCATGA GATGGACATT AAATATGATG GAATACACAT TCCAGGAAGT 
CCATTGCAAT TCTACGTGGA TTATGTCAAC AGCGGTCATG TGACTGCATA TGGTCCTGGT 
CTAATTCATG GCATGGTCAA TAAGCCTGCA GTCTTCACAG TCAACACAAA GGATGCTGGA 
GAGGGTGGTC TGTCTCTGGC CATCGAGGGG CCATCAAAGG CAGATATCAG CTGCACTGAT 
AATCAGGACG GTACCTGCAC TGTTTCATAC CTCCCTGTTC TTCCCGGAGA CTACAATATC 
ATTGTTAAAT ACAATGACAA GAACATCCCC GGCAGCCCTT TCATGTCCAA AATCACAGGT 
GACGACTCGA TGCGCATGTC TCACCTGAAG GTGGGCTCAG CTGCTGACAT CCCTCTGGAC 
ATTGGTGAGC TTGACCTCAG TCAGCTGACA GCCACACTCA CCACCCCATC CGGCCGAGAG 
GAGCCTTGCC TGCTCAAGAT GCTCAGAAAT GGGCATGTGG GAATTTCCTT TGTGCCCAAG 
GAAATTGGCG AGCATCTTGT TAACATTAAG AAAAATGGAC GCCATATTCC TAGTAGCCCC 
ATTTCTGTCA TGATCAACCA ATCAGAGATC GGTGATGCCA GCCGTGTACG AGTAACTGGT 
CAAGGCCTGA GTGAAGCTCG CACCTTTGAG CCCGCAGAAT TCATCATTGA CACTAGAGAT 
GCAGGATATG GTGGACTCAG TTTGTCTATT GAGGGACCTA GTAAAGTGGA TATCAACACT 
GAAGATCAGG AAGATGGCAC CTGCAAAGTG ACATATTGCC CCACAGAGCC TGGAAACTAC 
ATAATTAACA TCAAATTTGC AGACCAGCAT GTGCCAGGAA GTGCTTTCAC TGTGAAAGTG 
ACAGGCGAGG GTCGGATGAA GGAGAGCATC ACCCGCAAAA GAAGAGCTGC TTCTGTGGCC 
AATGTTGGCA GCCAGTGCGA CCTCAGTCTA AAGATCCCTG AGATCAGCAT TGCAGACATG 
ACCGCTCAGG TGACCAGCCC ATCTGGCAAA GTCCACAAGG CAGAGATAAT GGAGGGAGAA 
AACAACACCT ACTGCATCCG ATTCGTGCCC ACAGAGATGG GTGTGCACAC AGTAAGCGTG 
AAGTATCAAG GTCAGCATGT GCCTGGATCC CCCTTCCAGT TCACGGTGGG ACCTCTTGGA 
GAAGGAGGAG CTCATAAAGT CAGAGCTGGA GGCCCAGGAT TAGAGAGGGC AGAAGCTGGT 
GTACCAGCGG AGTTCAGCAT TTGGACTCGT GAAGCAGGAG CTGGTGGTCT GTCCATCGCA 
GTGGAAGGAC CCAGCAAAGC AGAGATTGCT TTTGAAGACC GTAAAGATGG ATCCAGTGGT 
GTCTCTTACA TCGTCCAGGA GCCTGGCGAT TATGAAGTAT CCATCAAGTT CAATGACGAG 
CACATCCCTG ACAGTCCGTT TGTGGTGCCT GTGGCCTCGC CATCTGACGA TGCCCGCCGT 
CTCACTGTTG CCAGTCTTCA GGAGTCCGGG TTAAAGGTGA ACCAGCCGGC CTCTTTTGCT 
GTGAGCCTAA ATGGGGCGAA AGGAGTCATC GATGCCAAAG TTCACAGTCC ATCAGGAGCA 
CTTGAAGAGT GCTGCGTCAC CGAGATCGAT GAAGATAAAT ATGCTGTGAG ATTCATTCCC 
AGGGAGAATG GATTGTACCT GATAGATGTG AAATTTAATG GTTCTCACAT CCCTGGAAGC 
CCCTTCAAGA TCCGTGTTGG TGAGACGGGT CAGGCTGGAG ATCCAGGAAT GGTGTCTGCT 
TATGGAGCTG GTCTGGAAGG AGGCACTACT GGATCGCCTT GTGAGTTCAT TGTAAACACC 
AGTTCAGCTG GCCCTGGAGC TTTGGCGGTG ACTATCGACG GGCCGTCCAA GGTGAAGATG 
GACTGTCAGG AATGCCCAGA GGGCTACAAA GTCACCTACA CACCTATGGC CCCTGGAAAC 
TACCTAATTT CTATTAAATA CGGTGGACCG TACCATATTG TTGGTAGTCC ATTCAAAGCT 
AAAATTACTG GCTCCCGATT GGTCAACAGC CACAGCATGC ATGAGACTTC ATCAGTACTG 
GTTGACCCTG TGACCCGCAG CATGACGACC AGCCAGCAGG CAGCGCCAGG CTGGGCCAGC 
TCTGATGCCA GCCGCGTAGT GGCCAAAGGT TTGGGGCTCA ACAAGGGCTT CATAGGACAG 
AAGAACACTT TCAGTGTGGA CTGTAGTAAA GCAGGGAGGA ACATGCTCCT GGTCGGAGTG 
GATGGACCCA AAGTGCCCTG TGAGGAGATC CTGGTGAAAC ACCTGGGCAA CCGCCTCTAC 
AACGTTTCCT ACCAGCTGAA GGAGAAAGGA GAGTACATTC TGGTGGTCAA ATGGGGTGAT 
GAGCACATCC CGGGCAGCCC GTATCACATC ACTGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

flna ( XM_021474523.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330198.1 Danio rerio filamin A, alpha (actin binding protein 280) (flna), transcript variant X1, mRNA.

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CloneID	ODa43841
Clone ID Related Accession (Same CDS sequence)	XM_009296770.2 , XM_021474523.1 , XM_009296770.3
Accession Version	XM_021474523.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	filamin-A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395361.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCAAC ACACACGCCC CCACCAGTCG AGCGCCGCCG CTCCCCTATC CAACGCTTCT 
CTCTCCCCGG ACAAGGACGC CGACATGCCC GCTACAGAGA AAGATCTCGC CGAGGACGCG 
CCATGGAAGA AAATCCAGCA AAATACATTC ACGCGTTGGT GCAACGAGCA TCTGAAATGT 
GTCAACAAGC GCATAGCGAA CCTGCAGACG GACCTGAGCG ACGGGCTCCG GCTGATCGCG 
CTTCTGGAGG TGCTCAGCCA GAAGAAGATG TTCCGCAAGT ACAACCAGCG CCCTACCTTC 
AGACAGATGC AGCTGGAGAA CGTCTCCGTG GCGCTGGAGT TCCTGGACAT TGAAAACATC 
AAGCTGGTCT CCATTGACAG TAAGGCCATA GTGGATGGTA ACCTGAAGCT GATCCTGGGT 
CTGATATGGA CTCTTATTCT GCATTATTCC ATCTCCATGC CCATGTGGGA CGAGGAAGAG 
GAGAGCGAGG AAAGCAAGCA GAAGACGCCC AAGCAGAGGC TCCTCGGATG GATCCAGAAC 
AAGCTGCCCG AGCTGCCAAT CACCAACTTC AGCCGCGATT GGCAGTCAGG GAAAGCCCTT 
GGCGCTCTGG TGGACAGTTG TGCTCCAGGT CTGTGTCCAG ACTGGGATTC CTGGGACCAG 
ACCAAGCCTG TGGACAATGC CAGAGAGGCC ATGCAGCAGG CTGATGACTG GCTCGGTGTT 
CCTCAGGTCA TCACACCAGA AGAAATCGTT GATCCCAATG TGGACGAGCA CTCCGTTATG 
ACTTACCTGT CCCAGTTTCC CAAAGCCAAA CTCAAGCCTG GTGCCCCTCT GAGGCCCAAA 
CTCAATCCCA AAAAGGCCCG TGCTTATGGA CCAGGTATTG AGCCAACAGG TAATGTTGTG 
ATGAAGAAGG CTGTGTTCAC TGTTGAGACC ATCAGCGCTG GTCAGGGTGA GGTACTGGTT 
TATGTCGAGG ACCCCGCTGG ACACCGCGAG GAGGCCAAAG TCACAGCCAA CAATGACAAG 
AACCGCACCT ACTCAGTATT CTATGTGCCA AAAGTCACTG GACAACACAA GGTGACAGTG 
CTGTTTGCAG GGCAACACAT CTCTAAGAGC CCGTTTGAGG TGGATGTGGG AATGGCTCAG 
GGAGACTCCA GCAAGGTCAC TGCCCAGGGC CCAGGACTCG AGCCTGCAGG CAACATTGCC 
AACAAAACCA CATACTTTGA TGTCTACACA GCTGGTGCTG GAGTAGGAGA GGTGGAGGTG 
GTCATCATGG ACCCCAGTGG AAAGAAGGAC ACGGTTGAGT GCAGTGTCGA GGATAAAGGC 
AACAGCAGCT ACCGATGCAC CTACAAGCCC ACTCAGGAGG GACAGCACAT CATTTACATC 
ACCTTTGCCG GGGGACAGAT CTCCAAGAGC CCTTACACTG TCAATGTGGG AGAGGCCTGT 
AATCCAAGCC TGTGTACAGC CAAGGGCCGC GGTCTGCAGC CTAAAGGCCT GAGGGTCAAG 
GAGACCGCCG AGTTTAAGGT TTACACCAAA GGAGCAGGAA CCGGAGACCT CAAAGTTACC 
ATCAAAGGGC CCAAGGGTCT CGAGGAGCCC TGTAAGAAGA AGGATTTGGG AGACGGCGTG 
TACAGCTTTG ATTATTACCC CTCCACACCT GGAAATTACA TCATCACCAT CACATGGGGC 
GGTCAACACA TCCCACGCAG CCCGTTTGAG GTGAAGATCG GGTCTGAAGC AGGACCGCAG 
CAGGTCAGGG CGTGGGGTCC AGGACTAGAG AGTGGCGTTG TGGGCAAATC TGCTGACTTT 
GTTGTGGAAG CTGTTGGTGA TGATGTGGGC ACTTTAGGTT TCTCTGTTGA AGGCCCTTCC 
CCGGCTAAGA TTGAATGTGA TGATAAGGGA GATGGTTCAT GTGACGTGAG GTACTGGCCC 
ACCGAACCGG GTGAATATGC AGTTCATGTG CTCTGTAAGA ATGAGGACAT CCAGCTCAGC 
CCTTTCATGG CTGAAATCAT GCCCGCCCCA GGAAAAGACT TTTATCCTGA CAAGGTGAAG 
GCTTATGGCC CCGGTCTGCA GAGCTCAGGC CTGTCAGTTG GCAAACCAGC CGAGTTTACC 
GTGGATGCCA AACTGGGTGG CAAAGCTCCT CTGAAGATTC AAGCTCAGGA CCGTGATGGA 
AACCCAGTCG ATGTGCAGGT GAAGGACAAT GGAAATGGGA CATACAGCTG CAGCTACACC 
CCTCGCAAAC CAGTCAAACA CACTGTGATG GTGTCCTGGG GCGGCGTCAA CATCCCAGAG 
AGCCCGTTCA GAATGAACAT TGGGGCAGGG TGTCATCCCA ATAAGGTGAA AGTATCTGGA 
CCTGGAGTGG CCAAGACAGG CCTCAAAGCA TATGATCCAA CATACTTTAC TGTGGATTGT 
GCTGAGGCTG GACAGGGCGA CATCAGCATA GGCATCAAAT GTGCTGCAGG TGTGGTGGGA 
CCTGCTGAGG CTGACATAGA CTTCGACATC ATTAGAAATG ACAACGACAC ATTTACTGTT 
AAATACACTC CTCCTGGAGC GGGCAGCTAC ACCATCATGG TGCTGTTTGC TGATCAGACC 
ATTCCCATGA CACCCATCAG AATTAAGGTT GATCCCTCTC ATGATGCAAG CAAGGTTAAA 
GCTGAGGGGC CTGGACTTAG CCGTTCTGGT GTGGAGCTGA ACAAACCCAC TCACTTCACT 
GTGAACACAA AGGCTGCAGG CAAGGCCAAG CTTGATGCTC AGTTCACTGG ACCCAACAAG 
GGAGATGCAG TCCGTGACTT CGACATAATC GACAACCATG ATGGCACTCA CACTGTCAAA 
TATACCCCTG TCCAACAGGG CAACATGGGT TTGAATGTCA CCTACGGTGG TGACCCTATT 
CCCAAGAGCC CATTTGCAGT AGCAATTGCC CCCTCTCTGG ACCTCAGTAA AGTTAAAGTG 
GCTGGTCTTG GAAGTAAAAT GACTGTTGGA AAGGATCAGG AGATCACAGT CAAATCTAAA 
GGTGCAGGCG GTCAGGGCAA AGTTGGCGCA AAGGTTACTG GTCCATCTGC TAAATCAGTG 
CCCTGTAAAG TTGAACCAGG CCTGAGCCCA GAAACCAGCC AAGTTCGTTT TATCCCAAGA 
GATAAGGGAC CCTATGAGGT GGAACTGACA TATGATGGCG CCCCCATCCC TGGCAGTCCA 
TTCCCCATCG AAGCCATTGC ACCTACTGAC CCATCCAAGG TGCGATGCTC TGGGCCAGGA 
CTGGAGCGGG CGAAGGTTGG CGAGCCTGGA CAGTTTGTGG TGGATTGCAC TAATGCTGGC 
CCTGCTGAGT TGACCATCGA GATCATCTCT GACAATGGCA CTGAGGCTGA AGTCCACATT 
CAAGACAACG GCGACGGGAC CTACACCATC ACTTACGTCC CTCTCTACCC TGGAGCCTAT 
ACCCTCACCA TCCGCTACGG GGACCAGGAT GTGCCAAACT TCCCAGCAAG ACTCCATGTG 
GAGCCTGCTG TGGAGACCAG TGGAGTGAAA GTGTTCGGAC CCGGAGTGGA GGGCAAAGGT 
GTTTTCAGGG AAGCCACTAC AGATTTCACT GTGGATGCCC GTGCGCTCAC TAAAACCGGA 
GGCAATCACA TCAAAACCTG CATCAACAAC CCATCGGGCA ACCGCACTGA AGCACTGATC 
CGAGATCTGG GAGACGGCAC ATATCAAGTG GAGTACACGC CTTATGAGGA GGGTACACAC 
AGTGTTGAGG TTGCATATGA CAGCTCCCCG GTTCCCAAAA GCCCCTTCCG TGTGCCAGTG 
ACCGAGGGCT GTGACCCGGC GCGTGTGCGT GTGCATGGTC CAGGACTTCA GTCTGGCATC 
ACCAACAAAC CTAACAAATT TACCGTGGAG ACCCGTGGGG CTGGTACTGG TGGACTGGGG 
CTGGCTATGG AAGGACCTTC TGAGGCCAAA ATGTCCTGCA CCGATAACAA AGATGGCAGC 
TGTTGTGTTG AATACATCCC ATATGAGCCA GGGACATACA ACCTCAATGT CACCTATGGA 
GGCCAACCAA TCACTGGAAG CCCTTTCTCT GTACCTGTCC ATGATACTGT TGACCCAACT 
AAAGTGAAAT GTCAGGGCCA GGGGCTTGGA AACAACGTGC GTGCCAATAT CCCACAGGTC 
TTTTCTGTGG ATGCAAGCAA GGCTGGTATG GCGCCACTGC AGGTCAGAGT TCAGGGACCC 
AAAGGTGTTG TGGAGCCTGC TGAGGTGGTG GATAATGGTG ACCAGACTCA CACAGTCAGC 
TATGTGCCCA CAAGAGAGGG GCCATACTCC ATTAATGTTC TGTATGCTGA TGAGGAGATC 
CCACAGAGTC CTTATAAGGT GAAGGTTCTG CCCACTCATG ACGCAAGTAA AGTCCGTTGC 
AGCGGTCCTG GCCTGAATAC CACTGGTGTT CCTGCCAGTC TGCCCGTGGA GTTCACTATT 
GACGCCAAAG ATGCAGGAGA GGGACTGTTG GCCGTCCAGA TAACTGACCC TGATGGGAAG 
CCAAAGAAGG CTAACATTCG TGATAATCAG GACGGGACCT ACCTTGTGTC CTATGTGCCT 
GACATGACCG GCCGTTACAC CATTCTTATT AAGTATGGTG GTGATGAGAT CCCGTATTCT 
CCATATCGCA TTCGAGCCCT GCCTACTGGA GACGCCAGCA AGTGTACAGT CACAGTCTCA 
ATTGGAGGTC ATGGTTTGGG AGCTGGAGTT GGTCCGACTA TTCAGATTGG AGAGCAGACT 
GTCATCACTG TGGATGCGAA GGCAGCTGGG AAGGGCAAGG TGACCTGTAC CGTGTGCACA 
CCTGATGGAG GCGAGGTGGA TGTGGACGTC GTGGAAAATG AGGATGGAAC GTTTGACATC 
TTTTACACTG CACCGCAGCC TGGGAAATAC GTGATCTGTG TGCGCTTCGG TGGAGAGCAC 
ATCCCTAACA GCCCCTTCCA AGTCACGGCT TTGGAAGGTG ACTCTCCTGA CCAACTAATG 
CAGCAGAGCC AGAACCTGCA GTACGCCTAC GCGCCCAACA TGGGCCAGAC ATGGGCTACA 
GATGGGCAAT TGGGTATGAA TGGACTGGAT GTAGCTGGAC TGAGACCCTT TGACTTGGTC 
ATTCCTTTCA CCATTCAGAA AGGAGAAATT ACAGGTGATG TACGAATGCC CTCTGGAAAA 
GTGGCCAAGC CTGATATTAC AGACAACAAG GATGGCACAG TCACAGTGAA ATATGCCCCA 
ACTGAGGCGG GCCTGCATGA GATGGACATT AAATATGATG GAATACACAT TCCAGGAAGT 
CCATTGCAAT TCTACGTGGA TTATGTCAAC AGCGGTCATG TGACTGCATA TGGTCCTGGT 
CTAATTCATG GCATGGTCAA TAAGCCTGCA GTCTTCACAG TCAACACAAA GGATGCTGGA 
GAGGGTGGTC TGTCTCTGGC CATCGAGGGG CCATCAAAGG CAGATATCAG CTGCACTGAT 
AATCAGGACG GTACCTGCAC TGTTTCATAC CTCCCTGTTC TTCCCGGAGA CTACAATATC 
ATTGTTAAAT ACAATGACAA GAACATCCCC GGCAGCCCTT TCATGTCCAA AATCACAGGT 
GACGACTCGA TGCGCATGTC TCACCTGAAG GTGGGCTCAG CTGCTGACAT CCCTCTGGAC 
ATTGGTGAGC TTGACCTCAG TCAGCTGACA GCCACACTCA CCACCCCATC CGGCCGAGAG 
GAGCCTTGCC TGCTCAAGAT GCTCAGAAAT GGGCATGTGG GAATTTCCTT TGTGCCCAAG 
GAAATTGGCG AGCATCTTGT TAACATTAAG AAAAATGGAC GCCATATTCC TAGTAGCCCC 
ATTTCTGTCA TGATCAACCA ATCAGAGATC GGTGATGCCA GCCGTGTACG AGTAACTGGT 
CAAGGCCTGA GTGAAGCTCG CACCTTTGAG CCCGCAGAAT TCATCATTGA CACTAGAGAT 
GCAGGATATG GTGGACTCAG TTTGTCTATT GAGGGACCTA GTAAAGTGGA TATCAACACT 
GAAGATCAGG AAGATGGCAC CTGCAAAGTG ACATATTGCC CCACAGAGCC TGGAAACTAC 
ATAATTAACA TCAAATTTGC AGACCAGCAT GTGCCAGGAA GTGCTTTCAC TGTGAAAGTG 
ACAGGCGAGG GTCGGATGAA GGAGAGCATC ACCCGCAAAA GAAGAGCTGC TTCTGTGGCC 
AATGTTGGCA GCCAGTGCGA CCTCAGTCTA AAGATCCCTG AGATCAGCAT TGCAGACATG 
ACCGCTCAGG TGACCAGCCC ATCTGGCAAA GTCCACAAGG CAGAGATAAT GGAGGGAGAA 
AACAACACCT ACTGCATCCG ATTCGTGCCC ACAGAGATGG GTGTGCACAC AGTAAGCGTG 
AAGTATCAAG GTCAGCATGT GCCTGGATCC CCCTTCCAGT TCACGGTGGG ACCTCTTGGA 
GAAGGAGGAG CTCATAAAGT CAGAGCTGGA GGCCCAGGAT TAGAGAGGGC AGAAGCTGGT 
GTACCAGCGG AGTTCAGCAT TTGGACTCGT GAAGCAGGAG CTGGTGGTCT GTCCATCGCA 
GTGGAAGGAC CCAGCAAAGC AGAGATTGCT TTTGAAGACC GTAAAGATGG ATCCAGTGGT 
GTCTCTTACA TCGTCCAGGA GCCTGGCGAT TATGAAGTAT CCATCAAGTT CAATGACGAG 
CACATCCCTG ACAGTCCGTT TGTGGTGCCT GTGGCCTCGC CATCTGACGA TGCCCGCCGT 
CTCACTGTTG CCAGTCTTCA GGAGTCCGGG TTAAAGGTGA ACCAGCCGGC CTCTTTTGCT 
GTGAGCCTAA ATGGGGCGAA AGGAGTCATC GATGCCAAAG TTCACAGTCC ATCAGGAGCA 
CTTGAAGAGT GCTGCGTCAC CGAGATCGAT GAAGATAAAT ATGCTGTGAG ATTCATTCCC 
AGGGAGAATG GATTGTACCT GATAGATGTG AAATTTAATG GTTCTCACAT CCCTGGAAGC 
CCCTTCAAGA TCCGTGTTGG TGAGACGGGT CAGGCTGGAG ATCCAGGAAT GGTGTCTGCT 
TATGGAGCTG GTCTGGAAGG AGGCACTACT GGATCGCCTT GTGAGTTCAT TGTAAACACC 
AGTTCAGCTG GCCCTGGAGC TTTGGCGGTG ACTATCGACG GGCCGTCCAA GGTGAAGATG 
GACTGTCAGG AATGCCCAGA GGGCTACAAA GTCACCTACA CACCTATGGC CCCTGGAAAC 
TACCTAATTT CTATTAAATA CGGTGGACCG TACCATATTG TTGGTAGTCC ATTCAAAGCT 
AAAATTACTG GCTCCCGATT GGTCAACAGC CACAGCATGC ATGAGACTTC ATCAGTACTG 
GTTGACCCTG TGACCCGCAG CATGACGACC AGCCAGCAGG CAGCGCCAGG CTGGGCCAGC 
TCTGATGCCA GCCGCGTAGT GGCCAAAGGT TTGGGGCTCA ACAAGGGCTT CATAGGACAG 
AAGAACACTT TCAGTGTGGA CTGTAGTAAA GCAGGGAGGA ACATGCTCCT GGTCGGAGTG 
GATGGACCCA AAGTGCCCTG TGAGGAGATC CTGGTGAAAC ACCTGGGCAA CCGCCTCTAC 
AACGTTTCCT ACCAGCTGAA GGAGAAAGGA GAGTACATTC TGGTGGTCAA ATGGGGTGAT 
GAGCACATCC CGGGCAGCCC GTATCACATC ACTGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330198.1
CDS	356..8014
Translation	MSQHTRPHQSSAAAPLSNASLSPDKDADMPATEKDLAEDAPWKKIQQNTFTRWCNEHLKCVNKRIANLQTDLSDGLRLIALLEVLSQKKMFRKYNQRPTFRQMQLENVSVALEFLDIENIKLVSIDSKAIVDGNLKLILGLIWTLILHYSISMPMWDEEEESEESKQKTPKQRLLGWIQNKLPELPITNFSRDWQSGKALGALVDSCAPGLCPDWDSWDQTKPVDNAREAMQQADDWLGVPQVITPEEIVDPNVDEHSVMTYLSQFPKAKLKPGAPLRPKLNPKKARAYGPGIEPTGNVVMKKAVFTVETISAGQGEVLVYVEDPAGHREEAKVTANNDKNRTYSVFYVPKVTGQHKVTVLFAGQHISKSPFEVDVGMAQGDSSKVTAQGPGLEPAGNIANKTTYFDVYTAGAGVGEVEVVIMDPSGKKDTVECSVEDKGNSSYRCTYKPTQEGQHIIYITFAGGQISKSPYTVNVGEACNPSLCTAKGRGLQPKGLRVKETAEFKVYTKGAGTGDLKVTIKGPKGLEEPCKKKDLGDGVYSFDYYPSTPGNYIITITWGGQHIPRSPFEVKIGSEAGPQQVRAWGPGLESGVVGKSADFVVEAVGDDVGTLGFSVEGPSPAKIECDDKGDGSCDVRYWPTEPGEYAVHVLCKNEDIQLSPFMAEIMPAPGKDFYPDKVKAYGPGLQSSGLSVGKPAEFTVDAKLGGKAPLKIQAQDRDGNPVDVQVKDNGNGTYSCSYTPRKPVKHTVMVSWGGVNIPESPFRMNIGAGCHPNKVKVSGPGVAKTGLKAYDPTYFTVDCAEAGQGDISIGIKCAAGVVGPAEADIDFDIIRNDNDTFTVKYTPPGAGSYTIMVLFADQTIPMTPIRIKVDPSHDASKVKAEGPGLSRSGVELNKPTHFTVNTKAAGKAKLDAQFTGPNKGDAVRDFDIIDNHDGTHTVKYTPVQQGNMGLNVTYGGDPIPKSPFAVAIAPSLDLSKVKVAGLGSKMTVGKDQEITVKSKGAGGQGKVGAKVTGPSAKSVPCKVEPGLSPETSQVRFIPRDKGPYEVELTYDGAPIPGSPFPIEAIAPTDPSKVRCSGPGLERAKVGEPGQFVVDCTNAGPAELTIEIISDNGTEAEVHIQDNGDGTYTITYVPLYPGAYTLTIRYGDQDVPNFPARLHVEPAVETSGVKVFGPGVEGKGVFREATTDFTVDARALTKTGGNHIKTCINNPSGNRTEALIRDLGDGTYQVEYTPYEEGTHSVEVAYDSSPVPKSPFRVPVTEGCDPARVRVHGPGLQSGITNKPNKFTVETRGAGTGGLGLAMEGPSEAKMSCTDNKDGSCCVEYIPYEPGTYNLNVTYGGQPITGSPFSVPVHDTVDPTKVKCQGQGLGNNVRANIPQVFSVDASKAGMAPLQVRVQGPKGVVEPAEVVDNGDQTHTVSYVPTREGPYSINVLYADEEIPQSPYKVKVLPTHDASKVRCSGPGLNTTGVPASLPVEFTIDAKDAGEGLLAVQITDPDGKPKKANIRDNQDGTYLVSYVPDMTGRYTILIKYGGDEIPYSPYRIRALPTGDASKCTVTVSIGGHGLGAGVGPTIQIGEQTVITVDAKAAGKGKVTCTVCTPDGGEVDVDVVENEDGTFDIFYTAPQPGKYVICVRFGGEHIPNSPFQVTALEGDSPDQLMQQSQNLQYAYAPNMGQTWATDGQLGMNGLDVAGLRPFDLVIPFTIQKGEITGDVRMPSGKVAKPDITDNKDGTVTVKYAPTEAGLHEMDIKYDGIHIPGSPLQFYVDYVNSGHVTAYGPGLIHGMVNKPAVFTVNTKDAGEGGLSLAIEGPSKADISCTDNQDGTCTVSYLPVLPGDYNIIVKYNDKNIPGSPFMSKITGDDSMRMSHLKVGSAADIPLDIGELDLSQLTATLTTPSGREEPCLLKMLRNGHVGISFVPKEIGEHLVNIKKNGRHIPSSPISVMINQSEIGDASRVRVTGQGLSEARTFEPAEFIIDTRDAGYGGLSLSIEGPSKVDINTEDQEDGTCKVTYCPTEPGNYIINIKFADQHVPGSAFTVKVTGEGRMKESITRKRRAASVANVGSQCDLSLKIPEISIADMTAQVTSPSGKVHKAEIMEGENNTYCIRFVPTEMGVHTVSVKYQGQHVPGSPFQFTVGPLGEGGAHKVRAGGPGLERAEAGVPAEFSIWTREAGAGGLSIAVEGPSKAEIAFEDRKDGSSGVSYIVQEPGDYEVSIKFNDEHIPDSPFVVPVASPSDDARRLTVASLQESGLKVNQPASFAVSLNGAKGVIDAKVHSPSGALEECCVTEIDEDKYAVRFIPRENGLYLIDVKFNGSHIPGSPFKIRVGETGQAGDPGMVSAYGAGLEGGTTGSPCEFIVNTSSAGPGALAVTIDGPSKVKMDCQECPEGYKVTYTPMAPGNYLISIKYGGPYHIVGSPFKAKITGSRLVNSHSMHETSSVLVDPVTRSMTTSQQAAPGWASSDASRVVAKGLGLNKGFIGQKNTFSVDCSKAGRNMLLVGVDGPKVPCEEILVKHLGNRLYNVSYQLKEKGEYILVVKWGDEHIPGSPYHITV

Target ORF information:

RefSeq Version	XM_021474523.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio filamin A, alpha (actin binding protein 280) (flna), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474523.1

ORF Insert Sequence:

ATGAGTCAAC ACACACGCCC CCACCAGTCG AGCGCCGCCG CTCCCCTATC CAACGCTTCT 
CTCTCCCCGG ACAAGGACGC CGACATGCCC GCTACAGAGA AAGATCTCGC CGAGGACGCG 
CCATGGAAGA AAATCCAGCA AAATACATTC ACGCGTTGGT GCAACGAGCA TCTGAAATGT 
GTCAACAAGC GCATAGCGAA CCTGCAGACG GACCTGAGCG ACGGGCTCCG GCTGATCGCG 
CTTCTGGAGG TGCTCAGCCA GAAGAAGATG TTCCGCAAGT ACAACCAGCG CCCTACCTTC 
AGACAGATGC AGCTGGAGAA CGTCTCCGTG GCGCTGGAGT TCCTGGACAT TGAAAACATC 
AAGCTGGTCT CCATTGACAG TAAGGCCATA GTGGATGGTA ACCTGAAGCT GATCCTGGGT 
CTGATATGGA CTCTTATTCT GCATTATTCC ATCTCCATGC CCATGTGGGA CGAGGAAGAG 
GAGAGCGAGG AAAGCAAGCA GAAGACGCCC AAGCAGAGGC TCCTCGGATG GATCCAGAAC 
AAGCTGCCCG AGCTGCCAAT CACCAACTTC AGCCGCGATT GGCAGTCAGG GAAAGCCCTT 
GGCGCTCTGG TGGACAGTTG TGCTCCAGGT CTGTGTCCAG ACTGGGATTC CTGGGACCAG 
ACCAAGCCTG TGGACAATGC CAGAGAGGCC ATGCAGCAGG CTGATGACTG GCTCGGTGTT 
CCTCAGGTCA TCACACCAGA AGAAATCGTT GATCCCAATG TGGACGAGCA CTCCGTTATG 
ACTTACCTGT CCCAGTTTCC CAAAGCCAAA CTCAAGCCTG GTGCCCCTCT GAGGCCCAAA 
CTCAATCCCA AAAAGGCCCG TGCTTATGGA CCAGGTATTG AGCCAACAGG TAATGTTGTG 
ATGAAGAAGG CTGTGTTCAC TGTTGAGACC ATCAGCGCTG GTCAGGGTGA GGTACTGGTT 
TATGTCGAGG ACCCCGCTGG ACACCGCGAG GAGGCCAAAG TCACAGCCAA CAATGACAAG 
AACCGCACCT ACTCAGTATT CTATGTGCCA AAAGTCACTG GACAACACAA GGTGACAGTG 
CTGTTTGCAG GGCAACACAT CTCTAAGAGC CCGTTTGAGG TGGATGTGGG AATGGCTCAG 
GGAGACTCCA GCAAGGTCAC TGCCCAGGGC CCAGGACTCG AGCCTGCAGG CAACATTGCC 
AACAAAACCA CATACTTTGA TGTCTACACA GCTGGTGCTG GAGTAGGAGA GGTGGAGGTG 
GTCATCATGG ACCCCAGTGG AAAGAAGGAC ACGGTTGAGT GCAGTGTCGA GGATAAAGGC 
AACAGCAGCT ACCGATGCAC CTACAAGCCC ACTCAGGAGG GACAGCACAT CATTTACATC 
ACCTTTGCCG GGGGACAGAT CTCCAAGAGC CCTTACACTG TCAATGTGGG AGAGGCCTGT 
AATCCAAGCC TGTGTACAGC CAAGGGCCGC GGTCTGCAGC CTAAAGGCCT GAGGGTCAAG 
GAGACCGCCG AGTTTAAGGT TTACACCAAA GGAGCAGGAA CCGGAGACCT CAAAGTTACC 
ATCAAAGGGC CCAAGGGTCT CGAGGAGCCC TGTAAGAAGA AGGATTTGGG AGACGGCGTG 
TACAGCTTTG ATTATTACCC CTCCACACCT GGAAATTACA TCATCACCAT CACATGGGGC 
GGTCAACACA TCCCACGCAG CCCGTTTGAG GTGAAGATCG GGTCTGAAGC AGGACCGCAG 
CAGGTCAGGG CGTGGGGTCC AGGACTAGAG AGTGGCGTTG TGGGCAAATC TGCTGACTTT 
GTTGTGGAAG CTGTTGGTGA TGATGTGGGC ACTTTAGGTT TCTCTGTTGA AGGCCCTTCC 
CCGGCTAAGA TTGAATGTGA TGATAAGGGA GATGGTTCAT GTGACGTGAG GTACTGGCCC 
ACCGAACCGG GTGAATATGC AGTTCATGTG CTCTGTAAGA ATGAGGACAT CCAGCTCAGC 
CCTTTCATGG CTGAAATCAT GCCCGCCCCA GGAAAAGACT TTTATCCTGA CAAGGTGAAG 
GCTTATGGCC CCGGTCTGCA GAGCTCAGGC CTGTCAGTTG GCAAACCAGC CGAGTTTACC 
GTGGATGCCA AACTGGGTGG CAAAGCTCCT CTGAAGATTC AAGCTCAGGA CCGTGATGGA 
AACCCAGTCG ATGTGCAGGT GAAGGACAAT GGAAATGGGA CATACAGCTG CAGCTACACC 
CCTCGCAAAC CAGTCAAACA CACTGTGATG GTGTCCTGGG GCGGCGTCAA CATCCCAGAG 
AGCCCGTTCA GAATGAACAT TGGGGCAGGG TGTCATCCCA ATAAGGTGAA AGTATCTGGA 
CCTGGAGTGG CCAAGACAGG CCTCAAAGCA TATGATCCAA CATACTTTAC TGTGGATTGT 
GCTGAGGCTG GACAGGGCGA CATCAGCATA GGCATCAAAT GTGCTGCAGG TGTGGTGGGA 
CCTGCTGAGG CTGACATAGA CTTCGACATC ATTAGAAATG ACAACGACAC ATTTACTGTT 
AAATACACTC CTCCTGGAGC GGGCAGCTAC ACCATCATGG TGCTGTTTGC TGATCAGACC 
ATTCCCATGA CACCCATCAG AATTAAGGTT GATCCCTCTC ATGATGCAAG CAAGGTTAAA 
GCTGAGGGGC CTGGACTTAG CCGTTCTGGT GTGGAGCTGA ACAAACCCAC TCACTTCACT 
GTGAACACAA AGGCTGCAGG CAAGGCCAAG CTTGATGCTC AGTTCACTGG ACCCAACAAG 
GGAGATGCAG TCCGTGACTT CGACATAATC GACAACCATG ATGGCACTCA CACTGTCAAA 
TATACCCCTG TCCAACAGGG CAACATGGGT TTGAATGTCA CCTACGGTGG TGACCCTATT 
CCCAAGAGCC CATTTGCAGT AGCAATTGCC CCCTCTCTGG ACCTCAGTAA AGTTAAAGTG 
GCTGGTCTTG GAAGTAAAAT GACTGTTGGA AAGGATCAGG AGATCACAGT CAAATCTAAA 
GGTGCAGGCG GTCAGGGCAA AGTTGGCGCA AAGGTTACTG GTCCATCTGC TAAATCAGTG 
CCCTGTAAAG TTGAACCAGG CCTGAGCCCA GAAACCAGCC AAGTTCGTTT TATCCCAAGA 
GATAAGGGAC CCTATGAGGT GGAACTGACA TATGATGGCG CCCCCATCCC TGGCAGTCCA 
TTCCCCATCG AAGCCATTGC ACCTACTGAC CCATCCAAGG TGCGATGCTC TGGGCCAGGA 
CTGGAGCGGG CGAAGGTTGG CGAGCCTGGA CAGTTTGTGG TGGATTGCAC TAATGCTGGC 
CCTGCTGAGT TGACCATCGA GATCATCTCT GACAATGGCA CTGAGGCTGA AGTCCACATT 
CAAGACAACG GCGACGGGAC CTACACCATC ACTTACGTCC CTCTCTACCC TGGAGCCTAT 
ACCCTCACCA TCCGCTACGG GGACCAGGAT GTGCCAAACT TCCCAGCAAG ACTCCATGTG 
GAGCCTGCTG TGGAGACCAG TGGAGTGAAA GTGTTCGGAC CCGGAGTGGA GGGCAAAGGT 
GTTTTCAGGG AAGCCACTAC AGATTTCACT GTGGATGCCC GTGCGCTCAC TAAAACCGGA 
GGCAATCACA TCAAAACCTG CATCAACAAC CCATCGGGCA ACCGCACTGA AGCACTGATC 
CGAGATCTGG GAGACGGCAC ATATCAAGTG GAGTACACGC CTTATGAGGA GGGTACACAC 
AGTGTTGAGG TTGCATATGA CAGCTCCCCG GTTCCCAAAA GCCCCTTCCG TGTGCCAGTG 
ACCGAGGGCT GTGACCCGGC GCGTGTGCGT GTGCATGGTC CAGGACTTCA GTCTGGCATC 
ACCAACAAAC CTAACAAATT TACCGTGGAG ACCCGTGGGG CTGGTACTGG TGGACTGGGG 
CTGGCTATGG AAGGACCTTC TGAGGCCAAA ATGTCCTGCA CCGATAACAA AGATGGCAGC 
TGTTGTGTTG AATACATCCC ATATGAGCCA GGGACATACA ACCTCAATGT CACCTATGGA 
GGCCAACCAA TCACTGGAAG CCCTTTCTCT GTACCTGTCC ATGATACTGT TGACCCAACT 
AAAGTGAAAT GTCAGGGCCA GGGGCTTGGA AACAACGTGC GTGCCAATAT CCCACAGGTC 
TTTTCTGTGG ATGCAAGCAA GGCTGGTATG GCGCCACTGC AGGTCAGAGT TCAGGGACCC 
AAAGGTGTTG TGGAGCCTGC TGAGGTGGTG GATAATGGTG ACCAGACTCA CACAGTCAGC 
TATGTGCCCA CAAGAGAGGG GCCATACTCC ATTAATGTTC TGTATGCTGA TGAGGAGATC 
CCACAGAGTC CTTATAAGGT GAAGGTTCTG CCCACTCATG ACGCAAGTAA AGTCCGTTGC 
AGCGGTCCTG GCCTGAATAC CACTGGTGTT CCTGCCAGTC TGCCCGTGGA GTTCACTATT 
GACGCCAAAG ATGCAGGAGA GGGACTGTTG GCCGTCCAGA TAACTGACCC TGATGGGAAG 
CCAAAGAAGG CTAACATTCG TGATAATCAG GACGGGACCT ACCTTGTGTC CTATGTGCCT 
GACATGACCG GCCGTTACAC CATTCTTATT AAGTATGGTG GTGATGAGAT CCCGTATTCT 
CCATATCGCA TTCGAGCCCT GCCTACTGGA GACGCCAGCA AGTGTACAGT CACAGTCTCA 
ATTGGAGGTC ATGGTTTGGG AGCTGGAGTT GGTCCGACTA TTCAGATTGG AGAGCAGACT 
GTCATCACTG TGGATGCGAA GGCAGCTGGG AAGGGCAAGG TGACCTGTAC CGTGTGCACA 
CCTGATGGAG GCGAGGTGGA TGTGGACGTC GTGGAAAATG AGGATGGAAC GTTTGACATC 
TTTTACACTG CACCGCAGCC TGGGAAATAC GTGATCTGTG TGCGCTTCGG TGGAGAGCAC 
ATCCCTAACA GCCCCTTCCA AGTCACGGCT TTGGAAGGTG ACTCTCCTGA CCAACTAATG 
CAGCAGAGCC AGAACCTGCA GTACGCCTAC GCGCCCAACA TGGGCCAGAC ATGGGCTACA 
GATGGGCAAT TGGGTATGAA TGGACTGGAT GTAGCTGGAC TGAGACCCTT TGACTTGGTC 
ATTCCTTTCA CCATTCAGAA AGGAGAAATT ACAGGTGATG TACGAATGCC CTCTGGAAAA 
GTGGCCAAGC CTGATATTAC AGACAACAAG GATGGCACAG TCACAGTGAA ATATGCCCCA 
ACTGAGGCGG GCCTGCATGA GATGGACATT AAATATGATG GAATACACAT TCCAGGAAGT 
CCATTGCAAT TCTACGTGGA TTATGTCAAC AGCGGTCATG TGACTGCATA TGGTCCTGGT 
CTAATTCATG GCATGGTCAA TAAGCCTGCA GTCTTCACAG TCAACACAAA GGATGCTGGA 
GAGGGTGGTC TGTCTCTGGC CATCGAGGGG CCATCAAAGG CAGATATCAG CTGCACTGAT 
AATCAGGACG GTACCTGCAC TGTTTCATAC CTCCCTGTTC TTCCCGGAGA CTACAATATC 
ATTGTTAAAT ACAATGACAA GAACATCCCC GGCAGCCCTT TCATGTCCAA AATCACAGGT 
GACGACTCGA TGCGCATGTC TCACCTGAAG GTGGGCTCAG CTGCTGACAT CCCTCTGGAC 
ATTGGTGAGC TTGACCTCAG TCAGCTGACA GCCACACTCA CCACCCCATC CGGCCGAGAG 
GAGCCTTGCC TGCTCAAGAT GCTCAGAAAT GGGCATGTGG GAATTTCCTT TGTGCCCAAG 
GAAATTGGCG AGCATCTTGT TAACATTAAG AAAAATGGAC GCCATATTCC TAGTAGCCCC 
ATTTCTGTCA TGATCAACCA ATCAGAGATC GGTGATGCCA GCCGTGTACG AGTAACTGGT 
CAAGGCCTGA GTGAAGCTCG CACCTTTGAG CCCGCAGAAT TCATCATTGA CACTAGAGAT 
GCAGGATATG GTGGACTCAG TTTGTCTATT GAGGGACCTA GTAAAGTGGA TATCAACACT 
GAAGATCAGG AAGATGGCAC CTGCAAAGTG ACATATTGCC CCACAGAGCC TGGAAACTAC 
ATAATTAACA TCAAATTTGC AGACCAGCAT GTGCCAGGAA GTGCTTTCAC TGTGAAAGTG 
ACAGGCGAGG GTCGGATGAA GGAGAGCATC ACCCGCAAAA GAAGAGCTGC TTCTGTGGCC 
AATGTTGGCA GCCAGTGCGA CCTCAGTCTA AAGATCCCTG AGATCAGCAT TGCAGACATG 
ACCGCTCAGG TGACCAGCCC ATCTGGCAAA GTCCACAAGG CAGAGATAAT GGAGGGAGAA 
AACAACACCT ACTGCATCCG ATTCGTGCCC ACAGAGATGG GTGTGCACAC AGTAAGCGTG 
AAGTATCAAG GTCAGCATGT GCCTGGATCC CCCTTCCAGT TCACGGTGGG ACCTCTTGGA 
GAAGGAGGAG CTCATAAAGT CAGAGCTGGA GGCCCAGGAT TAGAGAGGGC AGAAGCTGGT 
GTACCAGCGG AGTTCAGCAT TTGGACTCGT GAAGCAGGAG CTGGTGGTCT GTCCATCGCA 
GTGGAAGGAC CCAGCAAAGC AGAGATTGCT TTTGAAGACC GTAAAGATGG ATCCAGTGGT 
GTCTCTTACA TCGTCCAGGA GCCTGGCGAT TATGAAGTAT CCATCAAGTT CAATGACGAG 
CACATCCCTG ACAGTCCGTT TGTGGTGCCT GTGGCCTCGC CATCTGACGA TGCCCGCCGT 
CTCACTGTTG CCAGTCTTCA GGAGTCCGGG TTAAAGGTGA ACCAGCCGGC CTCTTTTGCT 
GTGAGCCTAA ATGGGGCGAA AGGAGTCATC GATGCCAAAG TTCACAGTCC ATCAGGAGCA 
CTTGAAGAGT GCTGCGTCAC CGAGATCGAT GAAGATAAAT ATGCTGTGAG ATTCATTCCC 
AGGGAGAATG GATTGTACCT GATAGATGTG AAATTTAATG GTTCTCACAT CCCTGGAAGC 
CCCTTCAAGA TCCGTGTTGG TGAGACGGGT CAGGCTGGAG ATCCAGGAAT GGTGTCTGCT 
TATGGAGCTG GTCTGGAAGG AGGCACTACT GGATCGCCTT GTGAGTTCAT TGTAAACACC 
AGTTCAGCTG GCCCTGGAGC TTTGGCGGTG ACTATCGACG GGCCGTCCAA GGTGAAGATG 
GACTGTCAGG AATGCCCAGA GGGCTACAAA GTCACCTACA CACCTATGGC CCCTGGAAAC 
TACCTAATTT CTATTAAATA CGGTGGACCG TACCATATTG TTGGTAGTCC ATTCAAAGCT 
AAAATTACTG GCTCCCGATT GGTCAACAGC CACAGCATGC ATGAGACTTC ATCAGTACTG 
GTTGACCCTG TGACCCGCAG CATGACGACC AGCCAGCAGG CAGCGCCAGG CTGGGCCAGC 
TCTGATGCCA GCCGCGTAGT GGCCAAAGGT TTGGGGCTCA ACAAGGGCTT CATAGGACAG 
AAGAACACTT TCAGTGTGGA CTGTAGTAAA GCAGGGAGGA ACATGCTCCT GGTCGGAGTG 
GATGGACCCA AAGTGCCCTG TGAGGAGATC CTGGTGAAAC ACCTGGGCAA CCGCCTCTAC 
AACGTTTCCT ACCAGCTGAA GGAGAAAGGA GAGTACATTC TGGTGGTCAA ATGGGGTGAT 
GAGCACATCC CGGGCAGCCC GTATCACATC ACTGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

flna ( XM_009296770.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295045.1 Danio rerio filamin A, alpha (actin binding protein 280) (flna), transcript variant X1, mRNA.

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CloneID	ODa43841
Clone ID Related Accession (Same CDS sequence)	XM_009296770.2 , XM_021474523.1 , XM_009296770.3
Accession Version	XM_009296770.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7659bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	filamin-A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296770.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCAAC ACACACGCCC CCACCAGTCG AGCGCCGCCG CTCCCCTATC CAACGCTTCT 
CTCTCCCCGG ACAAGGACGC CGACATGCCC GCTACAGAGA AAGATCTCGC CGAGGACGCG 
CCATGGAAGA AAATCCAGCA AAATACATTC ACGCGTTGGT GCAACGAGCA TCTGAAATGT 
GTCAACAAGC GCATAGCGAA CCTGCAGACG GACCTGAGCG ACGGGCTCCG GCTGATCGCG 
CTTCTGGAGG TGCTCAGCCA GAAGAAGATG TTCCGCAAGT ACAACCAGCG CCCTACCTTC 
AGACAGATGC AGCTGGAGAA CGTCTCCGTG GCGCTGGAGT TCCTGGACAT TGAAAACATC 
AAGCTGGTCT CCATTGACAG TAAGGCCATA GTGGATGGTA ACCTGAAGCT GATCCTGGGT 
CTGATATGGA CTCTTATTCT GCATTATTCC ATCTCCATGC CCATGTGGGA CGAGGAAGAG 
GAGAGCGAGG AAAGCAAGCA GAAGACGCCC AAGCAGAGGC TCCTCGGATG GATCCAGAAC 
AAGCTGCCCG AGCTGCCAAT CACCAACTTC AGCCGCGATT GGCAGTCAGG GAAAGCCCTT 
GGCGCTCTGG TGGACAGTTG TGCTCCAGGT CTGTGTCCAG ACTGGGATTC CTGGGACCAG 
ACCAAGCCTG TGGACAATGC CAGAGAGGCC ATGCAGCAGG CTGATGACTG GCTCGGTGTT 
CCTCAGGTCA TCACACCAGA AGAAATCGTT GATCCCAATG TGGACGAGCA CTCCGTTATG 
ACTTACCTGT CCCAGTTTCC CAAAGCCAAA CTCAAGCCTG GTGCCCCTCT GAGGCCCAAA 
CTCAATCCCA AAAAGGCCCG TGCTTATGGA CCAGGTATTG AGCCAACAGG TAATGTTGTG 
ATGAAGAAGG CTGTGTTCAC TGTTGAGACC ATCAGCGCTG GTCAGGGTGA GGTACTGGTT 
TATGTCGAGG ACCCCGCTGG ACACCGCGAG GAGGCCAAAG TCACAGCCAA CAATGACAAG 
AACCGCACCT ACTCAGTATT CTATGTGCCA AAAGTCACTG GACAACACAA GGTGACAGTG 
CTGTTTGCAG GGCAACACAT CTCTAAGAGC CCGTTTGAGG TGGATGTGGG AATGGCTCAG 
GGAGACTCCA GCAAGGTCAC TGCCCAGGGC CCAGGACTCG AGCCTGCAGG CAACATTGCC 
AACAAAACCA CATACTTTGA TGTCTACACA GCTGGTGCTG GAGTAGGAGA GGTGGAGGTG 
GTCATCATGG ACCCCAGTGG AAAGAAGGAC ACGGTTGAGT GCAGTGTCGA GGATAAAGGC 
AACAGCAGCT ACCGATGCAC CTACAAGCCC ACTCAGGAGG GACAGCACAT CATTTACATC 
ACCTTTGCCG GGGGACAGAT CTCCAAGAGC CCTTACACTG TCAATGTGGG AGAGGCCTGT 
AATCCAAGCC TGTGTACAGC CAAGGGCCGC GGTCTGCAGC CTAAAGGCCT GAGGGTCAAG 
GAGACCGCCG AGTTTAAGGT TTACACCAAA GGAGCAGGAA CCGGAGACCT CAAAGTTACC 
ATCAAAGGGC CCAAGGGTCT CGAGGAGCCC TGTAAGAAGA AGGATTTGGG AGACGGCGTG 
TACAGCTTTG ATTATTACCC CTCCACACCT GGAAATTACA TCATCACCAT CACATGGGGC 
GGTCAACACA TCCCACGCAG CCCGTTTGAG GTGAAGATCG GGTCTGAAGC AGGACCGCAG 
CAGGTCAGGG CGTGGGGTCC AGGACTAGAG AGTGGCGTTG TGGGCAAATC TGCTGACTTT 
GTTGTGGAAG CTGTTGGTGA TGATGTGGGC ACTTTAGGTT TCTCTGTTGA AGGCCCTTCC 
CCGGCTAAGA TTGAATGTGA TGATAAGGGA GATGGTTCAT GTGACGTGAG GTACTGGCCC 
ACCGAACCGG GTGAATATGC AGTTCATGTG CTCTGTAAGA ATGAGGACAT CCAGCTCAGC 
CCTTTCATGG CTGAAATCAT GCCCGCCCCA GGAAAAGACT TTTATCCTGA CAAGGTGAAG 
GCTTATGGCC CCGGTCTGCA GAGCTCAGGC CTGTCAGTTG GCAAACCAGC CGAGTTTACC 
GTGGATGCCA AACTGGGTGG CAAAGCTCCT CTGAAGATTC AAGCTCAGGA CCGTGATGGA 
AACCCAGTCG ATGTGCAGGT GAAGGACAAT GGAAATGGGA CATACAGCTG CAGCTACACC 
CCTCGCAAAC CAGTCAAACA CACTGTGATG GTGTCCTGGG GCGGCGTCAA CATCCCAGAG 
AGCCCGTTCA GAATGAACAT TGGGGCAGGG TGTCATCCCA ATAAGGTGAA AGTATCTGGA 
CCTGGAGTGG CCAAGACAGG CCTCAAAGCA TATGATCCAA CATACTTTAC TGTGGATTGT 
GCTGAGGCTG GACAGGGCGA CATCAGCATA GGCATCAAAT GTGCTGCAGG TGTGGTGGGA 
CCTGCTGAGG CTGACATAGA CTTCGACATC ATTAGAAATG ACAACGACAC ATTTACTGTT 
AAATACACTC CTCCTGGAGC GGGCAGCTAC ACCATCATGG TGCTGTTTGC TGATCAGACC 
ATTCCCATGA CACCCATCAG AATTAAGGTT GATCCCTCTC ATGATGCAAG CAAGGTTAAA 
GCTGAGGGGC CTGGACTTAG CCGTTCTGGT GTGGAGCTGA ACAAACCCAC TCACTTCACT 
GTGAACACAA AGGCTGCAGG CAAGGCCAAG CTTGATGCTC AGTTCACTGG ACCCAACAAG 
GGAGATGCAG TCCGTGACTT CGACATAATC GACAACCATG ATGGCACTCA CACTGTCAAA 
TATACCCCTG TCCAACAGGG CAACATGGGT TTGAATGTCA CCTACGGTGG TGACCCTATT 
CCCAAGAGCC CATTTGCAGT AGCAATTGCC CCCTCTCTGG ACCTCAGTAA AGTTAAAGTG 
GCTGGTCTTG GAAGTAAAAT GACTGTTGGA AAGGATCAGG AGATCACAGT CAAATCTAAA 
GGTGCAGGCG GTCAGGGCAA AGTTGGCGCA AAGGTTACTG GTCCATCTGC TAAATCAGTG 
CCCTGTAAAG TTGAACCAGG CCTGAGCCCA GAAACCAGCC AAGTTCGTTT TATCCCAAGA 
GATAAGGGAC CCTATGAGGT GGAACTGACA TATGATGGCG CCCCCATCCC TGGCAGTCCA 
TTCCCCATCG AAGCCATTGC ACCTACTGAC CCATCCAAGG TGCGATGCTC TGGGCCAGGA 
CTGGAGCGGG CGAAGGTTGG CGAGCCTGGA CAGTTTGTGG TGGATTGCAC TAATGCTGGC 
CCTGCTGAGT TGACCATCGA GATCATCTCT GACAATGGCA CTGAGGCTGA AGTCCACATT 
CAAGACAACG GCGACGGGAC CTACACCATC ACTTACGTCC CTCTCTACCC TGGAGCCTAT 
ACCCTCACCA TCCGCTACGG GGACCAGGAT GTGCCAAACT TCCCAGCAAG ACTCCATGTG 
GAGCCTGCTG TGGAGACCAG TGGAGTGAAA GTGTTCGGAC CCGGAGTGGA GGGCAAAGGT 
GTTTTCAGGG AAGCCACTAC AGATTTCACT GTGGATGCCC GTGCGCTCAC TAAAACCGGA 
GGCAATCACA TCAAAACCTG CATCAACAAC CCATCGGGCA ACCGCACTGA AGCACTGATC 
CGAGATCTGG GAGACGGCAC ATATCAAGTG GAGTACACGC CTTATGAGGA GGGTACACAC 
AGTGTTGAGG TTGCATATGA CAGCTCCCCG GTTCCCAAAA GCCCCTTCCG TGTGCCAGTG 
ACCGAGGGCT GTGACCCGGC GCGTGTGCGT GTGCATGGTC CAGGACTTCA GTCTGGCATC 
ACCAACAAAC CTAACAAATT TACCGTGGAG ACCCGTGGGG CTGGTACTGG TGGACTGGGG 
CTGGCTATGG AAGGACCTTC TGAGGCCAAA ATGTCCTGCA CCGATAACAA AGATGGCAGC 
TGTTGTGTTG AATACATCCC ATATGAGCCA GGGACATACA ACCTCAATGT CACCTATGGA 
GGCCAACCAA TCACTGGAAG CCCTTTCTCT GTACCTGTCC ATGATACTGT TGACCCAACT 
AAAGTGAAAT GTCAGGGCCA GGGGCTTGGA AACAACGTGC GTGCCAATAT CCCACAGGTC 
TTTTCTGTGG ATGCAAGCAA GGCTGGTATG GCGCCACTGC AGGTCAGAGT TCAGGGACCC 
AAAGGTGTTG TGGAGCCTGC TGAGGTGGTG GATAATGGTG ACCAGACTCA CACAGTCAGC 
TATGTGCCCA CAAGAGAGGG GCCATACTCC ATTAATGTTC TGTATGCTGA TGAGGAGATC 
CCACAGAGTC CTTATAAGGT GAAGGTTCTG CCCACTCATG ACGCAAGTAA AGTCCGTTGC 
AGCGGTCCTG GCCTGAATAC CACTGGTGTT CCTGCCAGTC TGCCCGTGGA GTTCACTATT 
GACGCCAAAG ATGCAGGAGA GGGACTGTTG GCCGTCCAGA TAACTGACCC TGATGGGAAG 
CCAAAGAAGG CTAACATTCG TGATAATCAG GACGGGACCT ACCTTGTGTC CTATGTGCCT 
GACATGACCG GCCGTTACAC CATTCTTATT AAGTATGGTG GTGATGAGAT CCCGTATTCT 
CCATATCGCA TTCGAGCCCT GCCTACTGGA GACGCCAGCA AGTGTACAGT CACAGTCTCA 
ATTGGAGGTC ATGGTTTGGG AGCTGGAGTT GGTCCGACTA TTCAGATTGG AGAGCAGACT 
GTCATCACTG TGGATGCGAA GGCAGCTGGG AAGGGCAAGG TGACCTGTAC CGTGTGCACA 
CCTGATGGAG GCGAGGTGGA TGTGGACGTC GTGGAAAATG AGGATGGAAC GTTTGACATC 
TTTTACACTG CACCGCAGCC TGGGAAATAC GTGATCTGTG TGCGCTTCGG TGGAGAGCAC 
ATCCCTAACA GCCCCTTCCA AGTCACGGCT TTGGAAGGTG ACTCTCCTGA CCAACTAATG 
CAGCAGAGCC AGAACCTGCA GTACGCCTAC GCGCCCAACA TGGGCCAGAC ATGGGCTACA 
GATGGGCAAT TGGGTATGAA TGGACTGGAT GTAGCTGGAC TGAGACCCTT TGACTTGGTC 
ATTCCTTTCA CCATTCAGAA AGGAGAAATT ACAGGTGATG TACGAATGCC CTCTGGAAAA 
GTGGCCAAGC CTGATATTAC AGACAACAAG GATGGCACAG TCACAGTGAA ATATGCCCCA 
ACTGAGGCGG GCCTGCATGA GATGGACATT AAATATGATG GAATACACAT TCCAGGAAGT 
CCATTGCAAT TCTACGTGGA TTATGTCAAC AGCGGTCATG TGACTGCATA TGGTCCTGGT 
CTAATTCATG GCATGGTCAA TAAGCCTGCA GTCTTCACAG TCAACACAAA GGATGCTGGA 
GAGGGTGGTC TGTCTCTGGC CATCGAGGGG CCATCAAAGG CAGATATCAG CTGCACTGAT 
AATCAGGACG GTACCTGCAC TGTTTCATAC CTCCCTGTTC TTCCCGGAGA CTACAATATC 
ATTGTTAAAT ACAATGACAA GAACATCCCC GGCAGCCCTT TCATGTCCAA AATCACAGGT 
GACGACTCGA TGCGCATGTC TCACCTGAAG GTGGGCTCAG CTGCTGACAT CCCTCTGGAC 
ATTGGTGAGC TTGACCTCAG TCAGCTGACA GCCACACTCA CCACCCCATC CGGCCGAGAG 
GAGCCTTGCC TGCTCAAGAT GCTCAGAAAT GGGCATGTGG GAATTTCCTT TGTGCCCAAG 
GAAATTGGCG AGCATCTTGT TAACATTAAG AAAAATGGAC GCCATATTCC TAGTAGCCCC 
ATTTCTGTCA TGATCAACCA ATCAGAGATC GGTGATGCCA GCCGTGTACG AGTAACTGGT 
CAAGGCCTGA GTGAAGCTCG CACCTTTGAG CCCGCAGAAT TCATCATTGA CACTAGAGAT 
GCAGGATATG GTGGACTCAG TTTGTCTATT GAGGGACCTA GTAAAGTGGA TATCAACACT 
GAAGATCAGG AAGATGGCAC CTGCAAAGTG ACATATTGCC CCACAGAGCC TGGAAACTAC 
ATAATTAACA TCAAATTTGC AGACCAGCAT GTGCCAGGAA GTGCTTTCAC TGTGAAAGTG 
ACAGGCGAGG GTCGGATGAA GGAGAGCATC ACCCGCAAAA GAAGAGCTGC TTCTGTGGCC 
AATGTTGGCA GCCAGTGCGA CCTCAGTCTA AAGATCCCTG AGATCAGCAT TGCAGACATG 
ACCGCTCAGG TGACCAGCCC ATCTGGCAAA GTCCACAAGG CAGAGATAAT GGAGGGAGAA 
AACAACACCT ACTGCATCCG ATTCGTGCCC ACAGAGATGG GTGTGCACAC AGTAAGCGTG 
AAGTATCAAG GTCAGCATGT GCCTGGATCC CCCTTCCAGT TCACGGTGGG ACCTCTTGGA 
GAAGGAGGAG CTCATAAAGT CAGAGCTGGA GGCCCAGGAT TAGAGAGGGC AGAAGCTGGT 
GTACCAGCGG AGTTCAGCAT TTGGACTCGT GAAGCAGGAG CTGGTGGTCT GTCCATCGCA 
GTGGAAGGAC CCAGCAAAGC AGAGATTGCT TTTGAAGACC GTAAAGATGG ATCCAGTGGT 
GTCTCTTACA TCGTCCAGGA GCCTGGCGAT TATGAAGTAT CCATCAAGTT CAATGACGAG 
CACATCCCTG ACAGTCCGTT TGTGGTGCCT GTGGCCTCGC CATCTGACGA TGCCCGCCGT 
CTCACTGTTG CCAGTCTTCA GGAGTCCGGG TTAAAGGTGA ACCAGCCGGC CTCTTTTGCT 
GTGAGCCTAA ATGGGGCGAA AGGAGTCATC GATGCCAAAG TTCACAGTCC ATCAGGAGCA 
CTTGAAGAGT GCTGCGTCAC CGAGATCGAT GAAGATAAAT ATGCTGTGAG ATTCATTCCC 
AGGGAGAATG GATTGTACCT GATAGATGTG AAATTTAATG GTTCTCACAT CCCTGGAAGC 
CCCTTCAAGA TCCGTGTTGG TGAGACGGGT CAGGCTGGAG ATCCAGGAAT GGTGTCTGCT 
TATGGAGCTG GTCTGGAAGG AGGCACTACT GGATCGCCTT GTGAGTTCAT TGTAAACACC 
AGTTCAGCTG GCCCTGGAGC TTTGGCGGTG ACTATCGACG GGCCGTCCAA GGTGAAGATG 
GACTGTCAGG AATGCCCAGA GGGCTACAAA GTCACCTACA CACCTATGGC CCCTGGAAAC 
TACCTAATTT CTATTAAATA CGGTGGACCG TACCATATTG TTGGTAGTCC ATTCAAAGCT 
AAAATTACTG GCTCCCGATT GGTCAACAGC CACAGCATGC ATGAGACTTC ATCAGTACTG 
GTTGACCCTG TGACCCGCAG CATGACGACC AGCCAGCAGG CAGCGCCAGG CTGGGCCAGC 
TCTGATGCCA GCCGCGTAGT GGCCAAAGGT TTGGGGCTCA ACAAGGGCTT CATAGGACAG 
AAGAACACTT TCAGTGTGGA CTGTAGTAAA GCAGGGAGGA ACATGCTCCT GGTCGGAGTG 
GATGGACCCA AAGTGCCCTG TGAGGAGATC CTGGTGAAAC ACCTGGGCAA CCGCCTCTAC 
AACGTTTCCT ACCAGCTGAA GGAGAAAGGA GAGTACATTC TGGTGGTCAA ATGGGGTGAT 
GAGCACATCC CGGGCAGCCC GTATCACATC ACTGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295045.1
CDS	356..8014
Translation	MSQHTRPHQSSAAAPLSNASLSPDKDADMPATEKDLAEDAPWKKIQQNTFTRWCNEHLKCVNKRIANLQTDLSDGLRLIALLEVLSQKKMFRKYNQRPTFRQMQLENVSVALEFLDIENIKLVSIDSKAIVDGNLKLILGLIWTLILHYSISMPMWDEEEESEESKQKTPKQRLLGWIQNKLPELPITNFSRDWQSGKALGALVDSCAPGLCPDWDSWDQTKPVDNAREAMQQADDWLGVPQVITPEEIVDPNVDEHSVMTYLSQFPKAKLKPGAPLRPKLNPKKARAYGPGIEPTGNVVMKKAVFTVETISAGQGEVLVYVEDPAGHREEAKVTANNDKNRTYSVFYVPKVTGQHKVTVLFAGQHISKSPFEVDVGMAQGDSSKVTAQGPGLEPAGNIANKTTYFDVYTAGAGVGEVEVVIMDPSGKKDTVECSVEDKGNSSYRCTYKPTQEGQHIIYITFAGGQISKSPYTVNVGEACNPSLCTAKGRGLQPKGLRVKETAEFKVYTKGAGTGDLKVTIKGPKGLEEPCKKKDLGDGVYSFDYYPSTPGNYIITITWGGQHIPRSPFEVKIGSEAGPQQVRAWGPGLESGVVGKSADFVVEAVGDDVGTLGFSVEGPSPAKIECDDKGDGSCDVRYWPTEPGEYAVHVLCKNEDIQLSPFMAEIMPAPGKDFYPDKVKAYGPGLQSSGLSVGKPAEFTVDAKLGGKAPLKIQAQDRDGNPVDVQVKDNGNGTYSCSYTPRKPVKHTVMVSWGGVNIPESPFRMNIGAGCHPNKVKVSGPGVAKTGLKAYDPTYFTVDCAEAGQGDISIGIKCAAGVVGPAEADIDFDIIRNDNDTFTVKYTPPGAGSYTIMVLFADQTIPMTPIRIKVDPSHDASKVKAEGPGLSRSGVELNKPTHFTVNTKAAGKAKLDAQFTGPNKGDAVRDFDIIDNHDGTHTVKYTPVQQGNMGLNVTYGGDPIPKSPFAVAIAPSLDLSKVKVAGLGSKMTVGKDQEITVKSKGAGGQGKVGAKVTGPSAKSVPCKVEPGLSPETSQVRFIPRDKGPYEVELTYDGAPIPGSPFPIEAIAPTDPSKVRCSGPGLERAKVGEPGQFVVDCTNAGPAELTIEIISDNGTEAEVHIQDNGDGTYTITYVPLYPGAYTLTIRYGDQDVPNFPARLHVEPAVETSGVKVFGPGVEGKGVFREATTDFTVDARALTKTGGNHIKTCINNPSGNRTEALIRDLGDGTYQVEYTPYEEGTHSVEVAYDSSPVPKSPFRVPVTEGCDPARVRVHGPGLQSGITNKPNKFTVETRGAGTGGLGLAMEGPSEAKMSCTDNKDGSCCVEYIPYEPGTYNLNVTYGGQPITGSPFSVPVHDTVDPTKVKCQGQGLGNNVRANIPQVFSVDASKAGMAPLQVRVQGPKGVVEPAEVVDNGDQTHTVSYVPTREGPYSINVLYADEEIPQSPYKVKVLPTHDASKVRCSGPGLNTTGVPASLPVEFTIDAKDAGEGLLAVQITDPDGKPKKANIRDNQDGTYLVSYVPDMTGRYTILIKYGGDEIPYSPYRIRALPTGDASKCTVTVSIGGHGLGAGVGPTIQIGEQTVITVDAKAAGKGKVTCTVCTPDGGEVDVDVVENEDGTFDIFYTAPQPGKYVICVRFGGEHIPNSPFQVTALEGDSPDQLMQQSQNLQYAYAPNMGQTWATDGQLGMNGLDVAGLRPFDLVIPFTIQKGEITGDVRMPSGKVAKPDITDNKDGTVTVKYAPTEAGLHEMDIKYDGIHIPGSPLQFYVDYVNSGHVTAYGPGLIHGMVNKPAVFTVNTKDAGEGGLSLAIEGPSKADISCTDNQDGTCTVSYLPVLPGDYNIIVKYNDKNIPGSPFMSKITGDDSMRMSHLKVGSAADIPLDIGELDLSQLTATLTTPSGREEPCLLKMLRNGHVGISFVPKEIGEHLVNIKKNGRHIPSSPISVMINQSEIGDASRVRVTGQGLSEARTFEPAEFIIDTRDAGYGGLSLSIEGPSKVDINTEDQEDGTCKVTYCPTEPGNYIINIKFADQHVPGSAFTVKVTGEGRMKESITRKRRAASVANVGSQCDLSLKIPEISIADMTAQVTSPSGKVHKAEIMEGENNTYCIRFVPTEMGVHTVSVKYQGQHVPGSPFQFTVGPLGEGGAHKVRAGGPGLERAEAGVPAEFSIWTREAGAGGLSIAVEGPSKAEIAFEDRKDGSSGVSYIVQEPGDYEVSIKFNDEHIPDSPFVVPVASPSDDARRLTVASLQESGLKVNQPASFAVSLNGAKGVIDAKVHSPSGALEECCVTEIDEDKYAVRFIPRENGLYLIDVKFNGSHIPGSPFKIRVGETGQAGDPGMVSAYGAGLEGGTTGSPCEFIVNTSSAGPGALAVTIDGPSKVKMDCQECPEGYKVTYTPMAPGNYLISIKYGGPYHIVGSPFKAKITGSRLVNSHSMHETSSVLVDPVTRSMTTSQQAAPGWASSDASRVVAKGLGLNKGFIGQKNTFSVDCSKAGRNMLLVGVDGPKVPCEEILVKHLGNRLYNVSYQLKEKGEYILVVKWGDEHIPGSPYHITV

Target ORF information:

RefSeq Version	XM_009296770.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio filamin A, alpha (actin binding protein 280) (flna), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296770.3

ORF Insert Sequence:

ATGAGTCAAC ACACACGCCC CCACCAGTCG AGCGCCGCCG CTCCCCTATC CAACGCTTCT 
CTCTCCCCGG ACAAGGACGC CGACATGCCC GCTACAGAGA AAGATCTCGC CGAGGACGCG 
CCATGGAAGA AAATCCAGCA AAATACATTC ACGCGTTGGT GCAACGAGCA TCTGAAATGT 
GTCAACAAGC GCATAGCGAA CCTGCAGACG GACCTGAGCG ACGGGCTCCG GCTGATCGCG 
CTTCTGGAGG TGCTCAGCCA GAAGAAGATG TTCCGCAAGT ACAACCAGCG CCCTACCTTC 
AGACAGATGC AGCTGGAGAA CGTCTCCGTG GCGCTGGAGT TCCTGGACAT TGAAAACATC 
AAGCTGGTCT CCATTGACAG TAAGGCCATA GTGGATGGTA ACCTGAAGCT GATCCTGGGT 
CTGATATGGA CTCTTATTCT GCATTATTCC ATCTCCATGC CCATGTGGGA CGAGGAAGAG 
GAGAGCGAGG AAAGCAAGCA GAAGACGCCC AAGCAGAGGC TCCTCGGATG GATCCAGAAC 
AAGCTGCCCG AGCTGCCAAT CACCAACTTC AGCCGCGATT GGCAGTCAGG GAAAGCCCTT 
GGCGCTCTGG TGGACAGTTG TGCTCCAGGT CTGTGTCCAG ACTGGGATTC CTGGGACCAG 
ACCAAGCCTG TGGACAATGC CAGAGAGGCC ATGCAGCAGG CTGATGACTG GCTCGGTGTT 
CCTCAGGTCA TCACACCAGA AGAAATCGTT GATCCCAATG TGGACGAGCA CTCCGTTATG 
ACTTACCTGT CCCAGTTTCC CAAAGCCAAA CTCAAGCCTG GTGCCCCTCT GAGGCCCAAA 
CTCAATCCCA AAAAGGCCCG TGCTTATGGA CCAGGTATTG AGCCAACAGG TAATGTTGTG 
ATGAAGAAGG CTGTGTTCAC TGTTGAGACC ATCAGCGCTG GTCAGGGTGA GGTACTGGTT 
TATGTCGAGG ACCCCGCTGG ACACCGCGAG GAGGCCAAAG TCACAGCCAA CAATGACAAG 
AACCGCACCT ACTCAGTATT CTATGTGCCA AAAGTCACTG GACAACACAA GGTGACAGTG 
CTGTTTGCAG GGCAACACAT CTCTAAGAGC CCGTTTGAGG TGGATGTGGG AATGGCTCAG 
GGAGACTCCA GCAAGGTCAC TGCCCAGGGC CCAGGACTCG AGCCTGCAGG CAACATTGCC 
AACAAAACCA CATACTTTGA TGTCTACACA GCTGGTGCTG GAGTAGGAGA GGTGGAGGTG 
GTCATCATGG ACCCCAGTGG AAAGAAGGAC ACGGTTGAGT GCAGTGTCGA GGATAAAGGC 
AACAGCAGCT ACCGATGCAC CTACAAGCCC ACTCAGGAGG GACAGCACAT CATTTACATC 
ACCTTTGCCG GGGGACAGAT CTCCAAGAGC CCTTACACTG TCAATGTGGG AGAGGCCTGT 
AATCCAAGCC TGTGTACAGC CAAGGGCCGC GGTCTGCAGC CTAAAGGCCT GAGGGTCAAG 
GAGACCGCCG AGTTTAAGGT TTACACCAAA GGAGCAGGAA CCGGAGACCT CAAAGTTACC 
ATCAAAGGGC CCAAGGGTCT CGAGGAGCCC TGTAAGAAGA AGGATTTGGG AGACGGCGTG 
TACAGCTTTG ATTATTACCC CTCCACACCT GGAAATTACA TCATCACCAT CACATGGGGC 
GGTCAACACA TCCCACGCAG CCCGTTTGAG GTGAAGATCG GGTCTGAAGC AGGACCGCAG 
CAGGTCAGGG CGTGGGGTCC AGGACTAGAG AGTGGCGTTG TGGGCAAATC TGCTGACTTT 
GTTGTGGAAG CTGTTGGTGA TGATGTGGGC ACTTTAGGTT TCTCTGTTGA AGGCCCTTCC 
CCGGCTAAGA TTGAATGTGA TGATAAGGGA GATGGTTCAT GTGACGTGAG GTACTGGCCC 
ACCGAACCGG GTGAATATGC AGTTCATGTG CTCTGTAAGA ATGAGGACAT CCAGCTCAGC 
CCTTTCATGG CTGAAATCAT GCCCGCCCCA GGAAAAGACT TTTATCCTGA CAAGGTGAAG 
GCTTATGGCC CCGGTCTGCA GAGCTCAGGC CTGTCAGTTG GCAAACCAGC CGAGTTTACC 
GTGGATGCCA AACTGGGTGG CAAAGCTCCT CTGAAGATTC AAGCTCAGGA CCGTGATGGA 
AACCCAGTCG ATGTGCAGGT GAAGGACAAT GGAAATGGGA CATACAGCTG CAGCTACACC 
CCTCGCAAAC CAGTCAAACA CACTGTGATG GTGTCCTGGG GCGGCGTCAA CATCCCAGAG 
AGCCCGTTCA GAATGAACAT TGGGGCAGGG TGTCATCCCA ATAAGGTGAA AGTATCTGGA 
CCTGGAGTGG CCAAGACAGG CCTCAAAGCA TATGATCCAA CATACTTTAC TGTGGATTGT 
GCTGAGGCTG GACAGGGCGA CATCAGCATA GGCATCAAAT GTGCTGCAGG TGTGGTGGGA 
CCTGCTGAGG CTGACATAGA CTTCGACATC ATTAGAAATG ACAACGACAC ATTTACTGTT 
AAATACACTC CTCCTGGAGC GGGCAGCTAC ACCATCATGG TGCTGTTTGC TGATCAGACC 
ATTCCCATGA CACCCATCAG AATTAAGGTT GATCCCTCTC ATGATGCAAG CAAGGTTAAA 
GCTGAGGGGC CTGGACTTAG CCGTTCTGGT GTGGAGCTGA ACAAACCCAC TCACTTCACT 
GTGAACACAA AGGCTGCAGG CAAGGCCAAG CTTGATGCTC AGTTCACTGG ACCCAACAAG 
GGAGATGCAG TCCGTGACTT CGACATAATC GACAACCATG ATGGCACTCA CACTGTCAAA 
TATACCCCTG TCCAACAGGG CAACATGGGT TTGAATGTCA CCTACGGTGG TGACCCTATT 
CCCAAGAGCC CATTTGCAGT AGCAATTGCC CCCTCTCTGG ACCTCAGTAA AGTTAAAGTG 
GCTGGTCTTG GAAGTAAAAT GACTGTTGGA AAGGATCAGG AGATCACAGT CAAATCTAAA 
GGTGCAGGCG GTCAGGGCAA AGTTGGCGCA AAGGTTACTG GTCCATCTGC TAAATCAGTG 
CCCTGTAAAG TTGAACCAGG CCTGAGCCCA GAAACCAGCC AAGTTCGTTT TATCCCAAGA 
GATAAGGGAC CCTATGAGGT GGAACTGACA TATGATGGCG CCCCCATCCC TGGCAGTCCA 
TTCCCCATCG AAGCCATTGC ACCTACTGAC CCATCCAAGG TGCGATGCTC TGGGCCAGGA 
CTGGAGCGGG CGAAGGTTGG CGAGCCTGGA CAGTTTGTGG TGGATTGCAC TAATGCTGGC 
CCTGCTGAGT TGACCATCGA GATCATCTCT GACAATGGCA CTGAGGCTGA AGTCCACATT 
CAAGACAACG GCGACGGGAC CTACACCATC ACTTACGTCC CTCTCTACCC TGGAGCCTAT 
ACCCTCACCA TCCGCTACGG GGACCAGGAT GTGCCAAACT TCCCAGCAAG ACTCCATGTG 
GAGCCTGCTG TGGAGACCAG TGGAGTGAAA GTGTTCGGAC CCGGAGTGGA GGGCAAAGGT 
GTTTTCAGGG AAGCCACTAC AGATTTCACT GTGGATGCCC GTGCGCTCAC TAAAACCGGA 
GGCAATCACA TCAAAACCTG CATCAACAAC CCATCGGGCA ACCGCACTGA AGCACTGATC 
CGAGATCTGG GAGACGGCAC ATATCAAGTG GAGTACACGC CTTATGAGGA GGGTACACAC 
AGTGTTGAGG TTGCATATGA CAGCTCCCCG GTTCCCAAAA GCCCCTTCCG TGTGCCAGTG 
ACCGAGGGCT GTGACCCGGC GCGTGTGCGT GTGCATGGTC CAGGACTTCA GTCTGGCATC 
ACCAACAAAC CTAACAAATT TACCGTGGAG ACCCGTGGGG CTGGTACTGG TGGACTGGGG 
CTGGCTATGG AAGGACCTTC TGAGGCCAAA ATGTCCTGCA CCGATAACAA AGATGGCAGC 
TGTTGTGTTG AATACATCCC ATATGAGCCA GGGACATACA ACCTCAATGT CACCTATGGA 
GGCCAACCAA TCACTGGAAG CCCTTTCTCT GTACCTGTCC ATGATACTGT TGACCCAACT 
AAAGTGAAAT GTCAGGGCCA GGGGCTTGGA AACAACGTGC GTGCCAATAT CCCACAGGTC 
TTTTCTGTGG ATGCAAGCAA GGCTGGTATG GCGCCACTGC AGGTCAGAGT TCAGGGACCC 
AAAGGTGTTG TGGAGCCTGC TGAGGTGGTG GATAATGGTG ACCAGACTCA CACAGTCAGC 
TATGTGCCCA CAAGAGAGGG GCCATACTCC ATTAATGTTC TGTATGCTGA TGAGGAGATC 
CCACAGAGTC CTTATAAGGT GAAGGTTCTG CCCACTCATG ACGCAAGTAA AGTCCGTTGC 
AGCGGTCCTG GCCTGAATAC CACTGGTGTT CCTGCCAGTC TGCCCGTGGA GTTCACTATT 
GACGCCAAAG ATGCAGGAGA GGGACTGTTG GCCGTCCAGA TAACTGACCC TGATGGGAAG 
CCAAAGAAGG CTAACATTCG TGATAATCAG GACGGGACCT ACCTTGTGTC CTATGTGCCT 
GACATGACCG GCCGTTACAC CATTCTTATT AAGTATGGTG GTGATGAGAT CCCGTATTCT 
CCATATCGCA TTCGAGCCCT GCCTACTGGA GACGCCAGCA AGTGTACAGT CACAGTCTCA 
ATTGGAGGTC ATGGTTTGGG AGCTGGAGTT GGTCCGACTA TTCAGATTGG AGAGCAGACT 
GTCATCACTG TGGATGCGAA GGCAGCTGGG AAGGGCAAGG TGACCTGTAC CGTGTGCACA 
CCTGATGGAG GCGAGGTGGA TGTGGACGTC GTGGAAAATG AGGATGGAAC GTTTGACATC 
TTTTACACTG CACCGCAGCC TGGGAAATAC GTGATCTGTG TGCGCTTCGG TGGAGAGCAC 
ATCCCTAACA GCCCCTTCCA AGTCACGGCT TTGGAAGGTG ACTCTCCTGA CCAACTAATG 
CAGCAGAGCC AGAACCTGCA GTACGCCTAC GCGCCCAACA TGGGCCAGAC ATGGGCTACA 
GATGGGCAAT TGGGTATGAA TGGACTGGAT GTAGCTGGAC TGAGACCCTT TGACTTGGTC 
ATTCCTTTCA CCATTCAGAA AGGAGAAATT ACAGGTGATG TACGAATGCC CTCTGGAAAA 
GTGGCCAAGC CTGATATTAC AGACAACAAG GATGGCACAG TCACAGTGAA ATATGCCCCA 
ACTGAGGCGG GCCTGCATGA GATGGACATT AAATATGATG GAATACACAT TCCAGGAAGT 
CCATTGCAAT TCTACGTGGA TTATGTCAAC AGCGGTCATG TGACTGCATA TGGTCCTGGT 
CTAATTCATG GCATGGTCAA TAAGCCTGCA GTCTTCACAG TCAACACAAA GGATGCTGGA 
GAGGGTGGTC TGTCTCTGGC CATCGAGGGG CCATCAAAGG CAGATATCAG CTGCACTGAT 
AATCAGGACG GTACCTGCAC TGTTTCATAC CTCCCTGTTC TTCCCGGAGA CTACAATATC 
ATTGTTAAAT ACAATGACAA GAACATCCCC GGCAGCCCTT TCATGTCCAA AATCACAGGT 
GACGACTCGA TGCGCATGTC TCACCTGAAG GTGGGCTCAG CTGCTGACAT CCCTCTGGAC 
ATTGGTGAGC TTGACCTCAG TCAGCTGACA GCCACACTCA CCACCCCATC CGGCCGAGAG 
GAGCCTTGCC TGCTCAAGAT GCTCAGAAAT GGGCATGTGG GAATTTCCTT TGTGCCCAAG 
GAAATTGGCG AGCATCTTGT TAACATTAAG AAAAATGGAC GCCATATTCC TAGTAGCCCC 
ATTTCTGTCA TGATCAACCA ATCAGAGATC GGTGATGCCA GCCGTGTACG AGTAACTGGT 
CAAGGCCTGA GTGAAGCTCG CACCTTTGAG CCCGCAGAAT TCATCATTGA CACTAGAGAT 
GCAGGATATG GTGGACTCAG TTTGTCTATT GAGGGACCTA GTAAAGTGGA TATCAACACT 
GAAGATCAGG AAGATGGCAC CTGCAAAGTG ACATATTGCC CCACAGAGCC TGGAAACTAC 
ATAATTAACA TCAAATTTGC AGACCAGCAT GTGCCAGGAA GTGCTTTCAC TGTGAAAGTG 
ACAGGCGAGG GTCGGATGAA GGAGAGCATC ACCCGCAAAA GAAGAGCTGC TTCTGTGGCC 
AATGTTGGCA GCCAGTGCGA CCTCAGTCTA AAGATCCCTG AGATCAGCAT TGCAGACATG 
ACCGCTCAGG TGACCAGCCC ATCTGGCAAA GTCCACAAGG CAGAGATAAT GGAGGGAGAA 
AACAACACCT ACTGCATCCG ATTCGTGCCC ACAGAGATGG GTGTGCACAC AGTAAGCGTG 
AAGTATCAAG GTCAGCATGT GCCTGGATCC CCCTTCCAGT TCACGGTGGG ACCTCTTGGA 
GAAGGAGGAG CTCATAAAGT CAGAGCTGGA GGCCCAGGAT TAGAGAGGGC AGAAGCTGGT 
GTACCAGCGG AGTTCAGCAT TTGGACTCGT GAAGCAGGAG CTGGTGGTCT GTCCATCGCA 
GTGGAAGGAC CCAGCAAAGC AGAGATTGCT TTTGAAGACC GTAAAGATGG ATCCAGTGGT 
GTCTCTTACA TCGTCCAGGA GCCTGGCGAT TATGAAGTAT CCATCAAGTT CAATGACGAG 
CACATCCCTG ACAGTCCGTT TGTGGTGCCT GTGGCCTCGC CATCTGACGA TGCCCGCCGT 
CTCACTGTTG CCAGTCTTCA GGAGTCCGGG TTAAAGGTGA ACCAGCCGGC CTCTTTTGCT 
GTGAGCCTAA ATGGGGCGAA AGGAGTCATC GATGCCAAAG TTCACAGTCC ATCAGGAGCA 
CTTGAAGAGT GCTGCGTCAC CGAGATCGAT GAAGATAAAT ATGCTGTGAG ATTCATTCCC 
AGGGAGAATG GATTGTACCT GATAGATGTG AAATTTAATG GTTCTCACAT CCCTGGAAGC 
CCCTTCAAGA TCCGTGTTGG TGAGACGGGT CAGGCTGGAG ATCCAGGAAT GGTGTCTGCT 
TATGGAGCTG GTCTGGAAGG AGGCACTACT GGATCGCCTT GTGAGTTCAT TGTAAACACC 
AGTTCAGCTG GCCCTGGAGC TTTGGCGGTG ACTATCGACG GGCCGTCCAA GGTGAAGATG 
GACTGTCAGG AATGCCCAGA GGGCTACAAA GTCACCTACA CACCTATGGC CCCTGGAAAC 
TACCTAATTT CTATTAAATA CGGTGGACCG TACCATATTG TTGGTAGTCC ATTCAAAGCT 
AAAATTACTG GCTCCCGATT GGTCAACAGC CACAGCATGC ATGAGACTTC ATCAGTACTG 
GTTGACCCTG TGACCCGCAG CATGACGACC AGCCAGCAGG CAGCGCCAGG CTGGGCCAGC 
TCTGATGCCA GCCGCGTAGT GGCCAAAGGT TTGGGGCTCA ACAAGGGCTT CATAGGACAG 
AAGAACACTT TCAGTGTGGA CTGTAGTAAA GCAGGGAGGA ACATGCTCCT GGTCGGAGTG 
GATGGACCCA AAGTGCCCTG TGAGGAGATC CTGGTGAAAC ACCTGGGCAA CCGCCTCTAC 
AACGTTTCCT ACCAGCTGAA GGAGAAAGGA GAGTACATTC TGGTGGTCAA ATGGGGTGAT 
GAGCACATCC CGGGCAGCCC GTATCACATC ACTGTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

flna ( XM_021474524.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330199.1 Danio rerio filamin A, alpha (actin binding protein 280) (flna), transcript variant X2, mRNA.

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CloneID	ODa43842
Clone ID Related Accession (Same CDS sequence)	XM_021474524.1 , XM_009296771.2 , XM_009296771.3
Accession Version	XM_021474524.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	filamin-A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395361.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCAAC ACACACGCCC CCACCAGTCG AGCGCCGCCG CTCCCCTATC CAACGCTTCT 
CTCTCCCCGG ACAAGGACGC CGACATGCCC GCTACAGAGA AAGATCTCGC CGAGGACGCG 
CCATGGAAGA AAATCCAGCA AAATACATTC ACGCGTTGGT GCAACGAGCA TCTGAAATGT 
GTCAACAAGC GCATAGCGAA CCTGCAGACG GACCTGAGCG ACGGGCTCCG GCTGATCGCG 
CTTCTGGAGG TGCTCAGCCA GAAGAAGATG TTCCGCAAGT ACAACCAGCG CCCTACCTTC 
AGACAGATGC AGCTGGAGAA CGTCTCCGTG GCGCTGGAGT TCCTGGACAT TGAAAACATC 
AAGCTGGTCT CCATTGACAG TAAGGCCATA GTGGATGGTA ACCTGAAGCT GATCCTGGGT 
CTGATATGGA CTCTTATTCT GCATTATTCC ATCTCCATGC CCATGTGGGA CGAGGAAGAG 
GAGAGCGAGG AAAGCAAGCA GAAGACGCCC AAGCAGAGGC TCCTCGGATG GATCCAGAAC 
AAGCTGCCCG AGCTGCCAAT CACCAACTTC AGCCGCGATT GGCAGTCAGG GAAAGCCCTT 
GGCGCTCTGG TGGACAGTTG TGCTCCAGGT CTGTGTCCAG ACTGGGATTC CTGGGACCAG 
ACCAAGCCTG TGGACAATGC CAGAGAGGCC ATGCAGCAGG CTGATGACTG GCTCGGTGTT 
CCTCAGGTCA TCACACCAGA AGAAATCGTT GATCCCAATG TGGACGAGCA CTCCGTTATG 
ACTTACCTGT CCCAGTTTCC CAAAGCCAAA CTCAAGCCTG GTGCCCCTCT GAGGCCCAAA 
CTCAATCCCA AAAAGGCCCG TGCTTATGGA CCAGGTATTG AGCCAACAGG TAATGTTGTG 
ATGAAGAAGG CTGTGTTCAC TGTTGAGACC ATCAGCGCTG GTCAGGGTGA GGTACTGGTT 
TATGTCGAGG ACCCCGCTGG ACACCGCGAG GAGGCCAAAG TCACAGCCAA CAATGACAAG 
AACCGCACCT ACTCAGTATT CTATGTGCCA AAAGTCACTG GACAACACAA GGTGACAGTG 
CTGTTTGCAG GGCAACACAT CTCTAAGAGC CCGTTTGAGG TGGATGTGGG AATGGCTCAG 
GGAGACTCCA GCAAGGTCAC TGCCCAGGGC CCAGGACTCG AGCCTGCAGG CAACATTGCC 
AACAAAACCA CATACTTTGA TGTCTACACA GCTGGTGCTG GAGTAGGAGA GGTGGAGGTG 
GTCATCATGG ACCCCAGTGG AAAGAAGGAC ACGGTTGAGT GCAGTGTCGA GGATAAAGGC 
AACAGCAGCT ACCGATGCAC CTACAAGCCC ACTCAGGAGG GACAGCACAT CATTTACATC 
ACCTTTGCCG GGGGACAGAT CTCCAAGAGC CCTTACACTG TCAATGTGGG AGAGGCCTGT 
AATCCAAGCC TGTGTACAGC CAAGGGCCGC GGTCTGCAGC CTAAAGGCCT GAGGGTCAAG 
GAGACCGCCG AGTTTAAGGT TTACACCAAA GGAGCAGGAA CCGGAGACCT CAAAGTTACC 
ATCAAAGGGC CCAAGGGTCT CGAGGAGCCC TGTAAGAAGA AGGATTTGGG AGACGGCGTG 
TACAGCTTTG ATTATTACCC CTCCACACCT GGAAATTACA TCATCACCAT CACATGGGGC 
GGTCAACACA TCCCACGCAG CCCGTTTGAG GTGAAGATCG GGTCTGAAGC AGGACCGCAG 
CAGGTCAGGG CGTGGGGTCC AGGACTAGAG AGTGGCGTTG TGGGCAAATC TGCTGACTTT 
GTTGTGGAAG CTGTTGGTGA TGATGTGGGC ACTTTAGGTT TCTCTGTTGA AGGCCCTTCC 
CCGGCTAAGA TTGAATGTGA TGATAAGGGA GATGGTTCAT GTGACGTGAG GTACTGGCCC 
ACCGAACCGG GTGAATATGC AGTTCATGTG CTCTGTAAGA ATGAGGACAT CCAGCTCAGC 
CCTTTCATGG CTGAAATCAT GCCCGCCCCA GGAAAAGACT TTTATCCTGA CAAGGTGAAG 
GCTTATGGCC CCGGTCTGCA GAGCTCAGGC CTGTCAGTTG GCAAACCAGC CGAGTTTACC 
GTGGATGCCA AACTGGGTGG CAAAGCTCCT CTGAAGATTC AAGCTCAGGA CCGTGATGGA 
AACCCAGTCG ATGTGCAGGT GAAGGACAAT GGAAATGGGA CATACAGCTG CAGCTACACC 
CCTCGCAAAC CAGTCAAACA CACTGTGATG GTGTCCTGGG GCGGCGTCAA CATCCCAGAG 
AGCCCGTTCA GAATGAACAT TGGGGCAGGG TGTCATCCCA ATAAGGTGAA AGTATCTGGA 
CCTGGAGTGG CCAAGACAGG CCTCAAAGCA TATGATCCAA CATACTTTAC TGTGGATTGT 
GCTGAGGCTG GACAGGGCGA CATCAGCATA GGCATCAAAT GTGCTGCAGG TGTGGTGGGA 
CCTGCTGAGG CTGACATAGA CTTCGACATC ATTAGAAATG ACAACGACAC ATTTACTGTT 
AAATACACTC CTCCTGGAGC GGGCAGCTAC ACCATCATGG TGCTGTTTGC TGATCAGACC 
ATTCCCATGA CACCCATCAG AATTAAGGTT GATCCCTCTC ATGATGCAAG CAAGGTTAAA 
GCTGAGGGGC CTGGACTTAG CCGTTCTGGT GTGGAGCTGA ACAAACCCAC TCACTTCACT 
GTGAACACAA AGGCTGCAGG CAAGGCCAAG CTTGATGCTC AGTTCACTGG ACCCAACAAG 
GGAGATGCAG TCCGTGACTT CGACATAATC GACAACCATG ATGGCACTCA CACTGTCAAA 
TATACCCCTG TCCAACAGGG CAACATGGGT TTGAATGTCA CCTACGGTGG TGACCCTATT 
CCCAAGAGCC CATTTGCAGT AGCAATTGCC CCCTCTCTGG ACCTCAGTAA AGTTAAAGTG 
GCTGGTCTTG GAAGTAAAAT GACTGTTGGA AAGGATCAGG AGATCACAGT CAAATCTAAA 
GGTGCAGGCG GTCAGGGCAA AGTTGGCGCA AAGGTTACTG GTCCATCTGC TAAATCAGTG 
CCCTGTAAAG TTGAACCAGG CCTGAGCCCA GAAACCAGCC AAGTTCGTTT TATCCCAAGA 
GATAAGGGAC CCTATGAGGT GGAACTGACA TATGATGGCG CCCCCATCCC TGGCAGTCCA 
TTCCCCATCG AAGCCATTGC ACCTACTGAC CCATCCAAGG TGCGATGCTC TGGGCCAGGA 
CTGGAGCGGG CGAAGGTTGG CGAGCCTGGA CAGTTTGTGG TGGATTGCAC TAATGCTGGC 
CCTGCTGAGT TGACCATCGA GATCATCTCT GACAATGGCA CTGAGGCTGA AGTCCACATT 
CAAGACAACG GCGACGGGAC CTACACCATC ACTTACGTCC CTCTCTACCC TGGAGCCTAT 
ACCCTCACCA TCCGCTACGG GGACCAGGAT GTGCCAAACT TCCCAGCAAG ACTCCATGTG 
GAGCCTGCTG TGGAGACCAG TGGAGTGAAA GTGTTCGGAC CCGGAGTGGA GGGCAAAGGT 
GTTTTCAGGG AAGCCACTAC AGATTTCACT GTGGATGCCC GTGCGCTCAC TAAAACCGGA 
GGCAATCACA TCAAAACCTG CATCAACAAC CCATCGGGCA ACCGCACTGA AGCACTGATC 
CGAGATCTGG GAGACGGCAC ATATCAAGTG GAGTACACGC CTTATGAGGA GGGTACACAC 
AGTGTTGAGG TTGCATATGA CAGCTCCCCG GTTCCCAAAA GCCCCTTCCG TGTGCCAGTG 
ACCGAGGGCT GTGACCCGGC GCGTGTGCGT GTGCATGGTC CAGGACTTCA GTCTGGCATC 
ACCAACAAAC CTAACAAATT TACCGTGGAG ACCCGTGGGG CTGGTACTGG TGGACTGGGG 
CTGGCTATGG AAGGACCTTC TGAGGCCAAA ATGTCCTGCA CCGATAACAA AGATGGCAGC 
TGTTGTGTTG AATACATCCC ATATGAGCCA GGGACATACA ACCTCAATGT CACCTATGGA 
GGCCAACCAA TCACTGGAAG CCCTTTCTCT GTACCTGTCC ATGATACTGT TGACCCAACT 
AAAGTGAAAT GTCAGGGCCA GGGGCTTGGA AACAACGTGC GTGCCAATAT CCCACAGGTC 
TTTTCTGTGG ATGCAAGCAA GGCTGGTATG GCGCCACTGC AGGTCAGAGT TCAGGGACCC 
AAAGGTGTTG TGGAGCCTGC TGAGGTGGTG GATAATGGTG ACCAGACTCA CACAGTCAGC 
TATGTGCCCA CAAGAGAGGG GCCATACTCC ATTAATGTTC TGTATGCTGA TGAGGAGATC 
CCACAGAGTC CTTATAAGGT GAAGGTTCTG CCCACTCATG ACGCAAGTAA AGTCCGTTGC 
AGCGGTCCTG GCCTGAATAC CACTGGTGTT CCTGCCAGTC TGCCCGTGGA GTTCACTATT 
GACGCCAAAG ATGCAGGAGA GGGACTGTTG GCCGTCCAGA TAACTGACCC TGATGGGAAG 
CCAAAGAAGG CTAACATTCG TGATAATCAG GACGGGACCT ACCTTGTGTC CTATGTGCCT 
GACATGACCG GCCGTTACAC CATTCTTATT AAGTATGGTG GTGATGAGAT CCCGTATTCT 
CCATATCGCA TTCGAGCCCT GCCTACTGGA GACGCCAGCA AGTGTACAGT CACAGGAGCT 
GGAGTTGGTC CGACTATTCA GATTGGAGAG CAGACTGTCA TCACTGTGGA TGCGAAGGCA 
GCTGGGAAGG GCAAGGTGAC CTGTACCGTG TGCACACCTG ATGGAGGCGA GGTGGATGTG 
GACGTCGTGG AAAATGAGGA TGGAACGTTT GACATCTTTT ACACTGCACC GCAGCCTGGG 
AAATACGTGA TCTGTGTGCG CTTCGGTGGA GAGCACATCC CTAACAGCCC CTTCCAAGTC 
ACGGCTTTGG AAGGTGACTC TCCTGACCAA CTAATGCAGC AGAGCCAGAA CCTGCAGTAC 
GCCTACGCGC CCAACATGGG CCAGACATGG GCTACAGATG GGCAATTGGG TATGAATGGA 
CTGGATGTAG CTGGACTGAG ACCCTTTGAC TTGGTCATTC CTTTCACCAT TCAGAAAGGA 
GAAATTACAG GTGATGTACG AATGCCCTCT GGAAAAGTGG CCAAGCCTGA TATTACAGAC 
AACAAGGATG GCACAGTCAC AGTGAAATAT GCCCCAACTG AGGCGGGCCT GCATGAGATG 
GACATTAAAT ATGATGGAAT ACACATTCCA GGAAGTCCAT TGCAATTCTA CGTGGATTAT 
GTCAACAGCG GTCATGTGAC TGCATATGGT CCTGGTCTAA TTCATGGCAT GGTCAATAAG 
CCTGCAGTCT TCACAGTCAA CACAAAGGAT GCTGGAGAGG GTGGTCTGTC TCTGGCCATC 
GAGGGGCCAT CAAAGGCAGA TATCAGCTGC ACTGATAATC AGGACGGTAC CTGCACTGTT 
TCATACCTCC CTGTTCTTCC CGGAGACTAC AATATCATTG TTAAATACAA TGACAAGAAC 
ATCCCCGGCA GCCCTTTCAT GTCCAAAATC ACAGGTGACG ACTCGATGCG CATGTCTCAC 
CTGAAGGTGG GCTCAGCTGC TGACATCCCT CTGGACATTG GTGAGCTTGA CCTCAGTCAG 
CTGACAGCCA CACTCACCAC CCCATCCGGC CGAGAGGAGC CTTGCCTGCT CAAGATGCTC 
AGAAATGGGC ATGTGGGAAT TTCCTTTGTG CCCAAGGAAA TTGGCGAGCA TCTTGTTAAC 
ATTAAGAAAA ATGGACGCCA TATTCCTAGT AGCCCCATTT CTGTCATGAT CAACCAATCA 
GAGATCGGTG ATGCCAGCCG TGTACGAGTA ACTGGTCAAG GCCTGAGTGA AGCTCGCACC 
TTTGAGCCCG CAGAATTCAT CATTGACACT AGAGATGCAG GATATGGTGG ACTCAGTTTG 
TCTATTGAGG GACCTAGTAA AGTGGATATC AACACTGAAG ATCAGGAAGA TGGCACCTGC 
AAAGTGACAT ATTGCCCCAC AGAGCCTGGA AACTACATAA TTAACATCAA ATTTGCAGAC 
CAGCATGTGC CAGGAAGTGC TTTCACTGTG AAAGTGACAG GCGAGGGTCG GATGAAGGAG 
AGCATCACCC GCAAAAGAAG AGCTGCTTCT GTGGCCAATG TTGGCAGCCA GTGCGACCTC 
AGTCTAAAGA TCCCTGAGAT CAGCATTGCA GACATGACCG CTCAGGTGAC CAGCCCATCT 
GGCAAAGTCC ACAAGGCAGA GATAATGGAG GGAGAAAACA ACACCTACTG CATCCGATTC 
GTGCCCACAG AGATGGGTGT GCACACAGTA AGCGTGAAGT ATCAAGGTCA GCATGTGCCT 
GGATCCCCCT TCCAGTTCAC GGTGGGACCT CTTGGAGAAG GAGGAGCTCA TAAAGTCAGA 
GCTGGAGGCC CAGGATTAGA GAGGGCAGAA GCTGGTGTAC CAGCGGAGTT CAGCATTTGG 
ACTCGTGAAG CAGGAGCTGG TGGTCTGTCC ATCGCAGTGG AAGGACCCAG CAAAGCAGAG 
ATTGCTTTTG AAGACCGTAA AGATGGATCC AGTGGTGTCT CTTACATCGT CCAGGAGCCT 
GGCGATTATG AAGTATCCAT CAAGTTCAAT GACGAGCACA TCCCTGACAG TCCGTTTGTG 
GTGCCTGTGG CCTCGCCATC TGACGATGCC CGCCGTCTCA CTGTTGCCAG TCTTCAGGAG 
TCCGGGTTAA AGGTGAACCA GCCGGCCTCT TTTGCTGTGA GCCTAAATGG GGCGAAAGGA 
GTCATCGATG CCAAAGTTCA CAGTCCATCA GGAGCACTTG AAGAGTGCTG CGTCACCGAG 
ATCGATGAAG ATAAATATGC TGTGAGATTC ATTCCCAGGG AGAATGGATT GTACCTGATA 
GATGTGAAAT TTAATGGTTC TCACATCCCT GGAAGCCCCT TCAAGATCCG TGTTGGTGAG 
ACGGGTCAGG CTGGAGATCC AGGAATGGTG TCTGCTTATG GAGCTGGTCT GGAAGGAGGC 
ACTACTGGAT CGCCTTGTGA GTTCATTGTA AACACCAGTT CAGCTGGCCC TGGAGCTTTG 
GCGGTGACTA TCGACGGGCC GTCCAAGGTG AAGATGGACT GTCAGGAATG CCCAGAGGGC 
TACAAAGTCA CCTACACACC TATGGCCCCT GGAAACTACC TAATTTCTAT TAAATACGGT 
GGACCGTACC ATATTGTTGG TAGTCCATTC AAAGCTAAAA TTACTGGCTC CCGATTGGTC 
AACAGCCACA GCATGCATGA GACTTCATCA GTACTGGTTG ACCCTGTGAC CCGCAGCATG 
ACGACCAGCC AGCAGGCAGC GCCAGGCTGG GCCAGCTCTG ATGCCAGCCG CGTAGTGGCC 
AAAGGTTTGG GGCTCAACAA GGGCTTCATA GGACAGAAGA ACACTTTCAG TGTGGACTGT 
AGTAAAGCAG GGAGGAACAT GCTCCTGGTC GGAGTGGATG GACCCAAAGT GCCCTGTGAG 
GAGATCCTGG TGAAACACCT GGGCAACCGC CTCTACAACG TTTCCTACCA GCTGAAGGAG 
AAAGGAGAGT ACATTCTGGT GGTCAAATGG GGTGATGAGC ACATCCCGGG CAGCCCGTAT 
CACATCACTG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330199.1
CDS	356..7990
Translation	MSQHTRPHQSSAAAPLSNASLSPDKDADMPATEKDLAEDAPWKKIQQNTFTRWCNEHLKCVNKRIANLQTDLSDGLRLIALLEVLSQKKMFRKYNQRPTFRQMQLENVSVALEFLDIENIKLVSIDSKAIVDGNLKLILGLIWTLILHYSISMPMWDEEEESEESKQKTPKQRLLGWIQNKLPELPITNFSRDWQSGKALGALVDSCAPGLCPDWDSWDQTKPVDNAREAMQQADDWLGVPQVITPEEIVDPNVDEHSVMTYLSQFPKAKLKPGAPLRPKLNPKKARAYGPGIEPTGNVVMKKAVFTVETISAGQGEVLVYVEDPAGHREEAKVTANNDKNRTYSVFYVPKVTGQHKVTVLFAGQHISKSPFEVDVGMAQGDSSKVTAQGPGLEPAGNIANKTTYFDVYTAGAGVGEVEVVIMDPSGKKDTVECSVEDKGNSSYRCTYKPTQEGQHIIYITFAGGQISKSPYTVNVGEACNPSLCTAKGRGLQPKGLRVKETAEFKVYTKGAGTGDLKVTIKGPKGLEEPCKKKDLGDGVYSFDYYPSTPGNYIITITWGGQHIPRSPFEVKIGSEAGPQQVRAWGPGLESGVVGKSADFVVEAVGDDVGTLGFSVEGPSPAKIECDDKGDGSCDVRYWPTEPGEYAVHVLCKNEDIQLSPFMAEIMPAPGKDFYPDKVKAYGPGLQSSGLSVGKPAEFTVDAKLGGKAPLKIQAQDRDGNPVDVQVKDNGNGTYSCSYTPRKPVKHTVMVSWGGVNIPESPFRMNIGAGCHPNKVKVSGPGVAKTGLKAYDPTYFTVDCAEAGQGDISIGIKCAAGVVGPAEADIDFDIIRNDNDTFTVKYTPPGAGSYTIMVLFADQTIPMTPIRIKVDPSHDASKVKAEGPGLSRSGVELNKPTHFTVNTKAAGKAKLDAQFTGPNKGDAVRDFDIIDNHDGTHTVKYTPVQQGNMGLNVTYGGDPIPKSPFAVAIAPSLDLSKVKVAGLGSKMTVGKDQEITVKSKGAGGQGKVGAKVTGPSAKSVPCKVEPGLSPETSQVRFIPRDKGPYEVELTYDGAPIPGSPFPIEAIAPTDPSKVRCSGPGLERAKVGEPGQFVVDCTNAGPAELTIEIISDNGTEAEVHIQDNGDGTYTITYVPLYPGAYTLTIRYGDQDVPNFPARLHVEPAVETSGVKVFGPGVEGKGVFREATTDFTVDARALTKTGGNHIKTCINNPSGNRTEALIRDLGDGTYQVEYTPYEEGTHSVEVAYDSSPVPKSPFRVPVTEGCDPARVRVHGPGLQSGITNKPNKFTVETRGAGTGGLGLAMEGPSEAKMSCTDNKDGSCCVEYIPYEPGTYNLNVTYGGQPITGSPFSVPVHDTVDPTKVKCQGQGLGNNVRANIPQVFSVDASKAGMAPLQVRVQGPKGVVEPAEVVDNGDQTHTVSYVPTREGPYSINVLYADEEIPQSPYKVKVLPTHDASKVRCSGPGLNTTGVPASLPVEFTIDAKDAGEGLLAVQITDPDGKPKKANIRDNQDGTYLVSYVPDMTGRYTILIKYGGDEIPYSPYRIRALPTGDASKCTVTGAGVGPTIQIGEQTVITVDAKAAGKGKVTCTVCTPDGGEVDVDVVENEDGTFDIFYTAPQPGKYVICVRFGGEHIPNSPFQVTALEGDSPDQLMQQSQNLQYAYAPNMGQTWATDGQLGMNGLDVAGLRPFDLVIPFTIQKGEITGDVRMPSGKVAKPDITDNKDGTVTVKYAPTEAGLHEMDIKYDGIHIPGSPLQFYVDYVNSGHVTAYGPGLIHGMVNKPAVFTVNTKDAGEGGLSLAIEGPSKADISCTDNQDGTCTVSYLPVLPGDYNIIVKYNDKNIPGSPFMSKITGDDSMRMSHLKVGSAADIPLDIGELDLSQLTATLTTPSGREEPCLLKMLRNGHVGISFVPKEIGEHLVNIKKNGRHIPSSPISVMINQSEIGDASRVRVTGQGLSEARTFEPAEFIIDTRDAGYGGLSLSIEGPSKVDINTEDQEDGTCKVTYCPTEPGNYIINIKFADQHVPGSAFTVKVTGEGRMKESITRKRRAASVANVGSQCDLSLKIPEISIADMTAQVTSPSGKVHKAEIMEGENNTYCIRFVPTEMGVHTVSVKYQGQHVPGSPFQFTVGPLGEGGAHKVRAGGPGLERAEAGVPAEFSIWTREAGAGGLSIAVEGPSKAEIAFEDRKDGSSGVSYIVQEPGDYEVSIKFNDEHIPDSPFVVPVASPSDDARRLTVASLQESGLKVNQPASFAVSLNGAKGVIDAKVHSPSGALEECCVTEIDEDKYAVRFIPRENGLYLIDVKFNGSHIPGSPFKIRVGETGQAGDPGMVSAYGAGLEGGTTGSPCEFIVNTSSAGPGALAVTIDGPSKVKMDCQECPEGYKVTYTPMAPGNYLISIKYGGPYHIVGSPFKAKITGSRLVNSHSMHETSSVLVDPVTRSMTTSQQAAPGWASSDASRVVAKGLGLNKGFIGQKNTFSVDCSKAGRNMLLVGVDGPKVPCEEILVKHLGNRLYNVSYQLKEKGEYILVVKWGDEHIPGSPYHITV

Target ORF information:

RefSeq Version	XM_021474524.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio filamin A, alpha (actin binding protein 280) (flna), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474524.1

ORF Insert Sequence:

ATGAGTCAAC ACACACGCCC CCACCAGTCG AGCGCCGCCG CTCCCCTATC CAACGCTTCT 
CTCTCCCCGG ACAAGGACGC CGACATGCCC GCTACAGAGA AAGATCTCGC CGAGGACGCG 
CCATGGAAGA AAATCCAGCA AAATACATTC ACGCGTTGGT GCAACGAGCA TCTGAAATGT 
GTCAACAAGC GCATAGCGAA CCTGCAGACG GACCTGAGCG ACGGGCTCCG GCTGATCGCG 
CTTCTGGAGG TGCTCAGCCA GAAGAAGATG TTCCGCAAGT ACAACCAGCG CCCTACCTTC 
AGACAGATGC AGCTGGAGAA CGTCTCCGTG GCGCTGGAGT TCCTGGACAT TGAAAACATC 
AAGCTGGTCT CCATTGACAG TAAGGCCATA GTGGATGGTA ACCTGAAGCT GATCCTGGGT 
CTGATATGGA CTCTTATTCT GCATTATTCC ATCTCCATGC CCATGTGGGA CGAGGAAGAG 
GAGAGCGAGG AAAGCAAGCA GAAGACGCCC AAGCAGAGGC TCCTCGGATG GATCCAGAAC 
AAGCTGCCCG AGCTGCCAAT CACCAACTTC AGCCGCGATT GGCAGTCAGG GAAAGCCCTT 
GGCGCTCTGG TGGACAGTTG TGCTCCAGGT CTGTGTCCAG ACTGGGATTC CTGGGACCAG 
ACCAAGCCTG TGGACAATGC CAGAGAGGCC ATGCAGCAGG CTGATGACTG GCTCGGTGTT 
CCTCAGGTCA TCACACCAGA AGAAATCGTT GATCCCAATG TGGACGAGCA CTCCGTTATG 
ACTTACCTGT CCCAGTTTCC CAAAGCCAAA CTCAAGCCTG GTGCCCCTCT GAGGCCCAAA 
CTCAATCCCA AAAAGGCCCG TGCTTATGGA CCAGGTATTG AGCCAACAGG TAATGTTGTG 
ATGAAGAAGG CTGTGTTCAC TGTTGAGACC ATCAGCGCTG GTCAGGGTGA GGTACTGGTT 
TATGTCGAGG ACCCCGCTGG ACACCGCGAG GAGGCCAAAG TCACAGCCAA CAATGACAAG 
AACCGCACCT ACTCAGTATT CTATGTGCCA AAAGTCACTG GACAACACAA GGTGACAGTG 
CTGTTTGCAG GGCAACACAT CTCTAAGAGC CCGTTTGAGG TGGATGTGGG AATGGCTCAG 
GGAGACTCCA GCAAGGTCAC TGCCCAGGGC CCAGGACTCG AGCCTGCAGG CAACATTGCC 
AACAAAACCA CATACTTTGA TGTCTACACA GCTGGTGCTG GAGTAGGAGA GGTGGAGGTG 
GTCATCATGG ACCCCAGTGG AAAGAAGGAC ACGGTTGAGT GCAGTGTCGA GGATAAAGGC 
AACAGCAGCT ACCGATGCAC CTACAAGCCC ACTCAGGAGG GACAGCACAT CATTTACATC 
ACCTTTGCCG GGGGACAGAT CTCCAAGAGC CCTTACACTG TCAATGTGGG AGAGGCCTGT 
AATCCAAGCC TGTGTACAGC CAAGGGCCGC GGTCTGCAGC CTAAAGGCCT GAGGGTCAAG 
GAGACCGCCG AGTTTAAGGT TTACACCAAA GGAGCAGGAA CCGGAGACCT CAAAGTTACC 
ATCAAAGGGC CCAAGGGTCT CGAGGAGCCC TGTAAGAAGA AGGATTTGGG AGACGGCGTG 
TACAGCTTTG ATTATTACCC CTCCACACCT GGAAATTACA TCATCACCAT CACATGGGGC 
GGTCAACACA TCCCACGCAG CCCGTTTGAG GTGAAGATCG GGTCTGAAGC AGGACCGCAG 
CAGGTCAGGG CGTGGGGTCC AGGACTAGAG AGTGGCGTTG TGGGCAAATC TGCTGACTTT 
GTTGTGGAAG CTGTTGGTGA TGATGTGGGC ACTTTAGGTT TCTCTGTTGA AGGCCCTTCC 
CCGGCTAAGA TTGAATGTGA TGATAAGGGA GATGGTTCAT GTGACGTGAG GTACTGGCCC 
ACCGAACCGG GTGAATATGC AGTTCATGTG CTCTGTAAGA ATGAGGACAT CCAGCTCAGC 
CCTTTCATGG CTGAAATCAT GCCCGCCCCA GGAAAAGACT TTTATCCTGA CAAGGTGAAG 
GCTTATGGCC CCGGTCTGCA GAGCTCAGGC CTGTCAGTTG GCAAACCAGC CGAGTTTACC 
GTGGATGCCA AACTGGGTGG CAAAGCTCCT CTGAAGATTC AAGCTCAGGA CCGTGATGGA 
AACCCAGTCG ATGTGCAGGT GAAGGACAAT GGAAATGGGA CATACAGCTG CAGCTACACC 
CCTCGCAAAC CAGTCAAACA CACTGTGATG GTGTCCTGGG GCGGCGTCAA CATCCCAGAG 
AGCCCGTTCA GAATGAACAT TGGGGCAGGG TGTCATCCCA ATAAGGTGAA AGTATCTGGA 
CCTGGAGTGG CCAAGACAGG CCTCAAAGCA TATGATCCAA CATACTTTAC TGTGGATTGT 
GCTGAGGCTG GACAGGGCGA CATCAGCATA GGCATCAAAT GTGCTGCAGG TGTGGTGGGA 
CCTGCTGAGG CTGACATAGA CTTCGACATC ATTAGAAATG ACAACGACAC ATTTACTGTT 
AAATACACTC CTCCTGGAGC GGGCAGCTAC ACCATCATGG TGCTGTTTGC TGATCAGACC 
ATTCCCATGA CACCCATCAG AATTAAGGTT GATCCCTCTC ATGATGCAAG CAAGGTTAAA 
GCTGAGGGGC CTGGACTTAG CCGTTCTGGT GTGGAGCTGA ACAAACCCAC TCACTTCACT 
GTGAACACAA AGGCTGCAGG CAAGGCCAAG CTTGATGCTC AGTTCACTGG ACCCAACAAG 
GGAGATGCAG TCCGTGACTT CGACATAATC GACAACCATG ATGGCACTCA CACTGTCAAA 
TATACCCCTG TCCAACAGGG CAACATGGGT TTGAATGTCA CCTACGGTGG TGACCCTATT 
CCCAAGAGCC CATTTGCAGT AGCAATTGCC CCCTCTCTGG ACCTCAGTAA AGTTAAAGTG 
GCTGGTCTTG GAAGTAAAAT GACTGTTGGA AAGGATCAGG AGATCACAGT CAAATCTAAA 
GGTGCAGGCG GTCAGGGCAA AGTTGGCGCA AAGGTTACTG GTCCATCTGC TAAATCAGTG 
CCCTGTAAAG TTGAACCAGG CCTGAGCCCA GAAACCAGCC AAGTTCGTTT TATCCCAAGA 
GATAAGGGAC CCTATGAGGT GGAACTGACA TATGATGGCG CCCCCATCCC TGGCAGTCCA 
TTCCCCATCG AAGCCATTGC ACCTACTGAC CCATCCAAGG TGCGATGCTC TGGGCCAGGA 
CTGGAGCGGG CGAAGGTTGG CGAGCCTGGA CAGTTTGTGG TGGATTGCAC TAATGCTGGC 
CCTGCTGAGT TGACCATCGA GATCATCTCT GACAATGGCA CTGAGGCTGA AGTCCACATT 
CAAGACAACG GCGACGGGAC CTACACCATC ACTTACGTCC CTCTCTACCC TGGAGCCTAT 
ACCCTCACCA TCCGCTACGG GGACCAGGAT GTGCCAAACT TCCCAGCAAG ACTCCATGTG 
GAGCCTGCTG TGGAGACCAG TGGAGTGAAA GTGTTCGGAC CCGGAGTGGA GGGCAAAGGT 
GTTTTCAGGG AAGCCACTAC AGATTTCACT GTGGATGCCC GTGCGCTCAC TAAAACCGGA 
GGCAATCACA TCAAAACCTG CATCAACAAC CCATCGGGCA ACCGCACTGA AGCACTGATC 
CGAGATCTGG GAGACGGCAC ATATCAAGTG GAGTACACGC CTTATGAGGA GGGTACACAC 
AGTGTTGAGG TTGCATATGA CAGCTCCCCG GTTCCCAAAA GCCCCTTCCG TGTGCCAGTG 
ACCGAGGGCT GTGACCCGGC GCGTGTGCGT GTGCATGGTC CAGGACTTCA GTCTGGCATC 
ACCAACAAAC CTAACAAATT TACCGTGGAG ACCCGTGGGG CTGGTACTGG TGGACTGGGG 
CTGGCTATGG AAGGACCTTC TGAGGCCAAA ATGTCCTGCA CCGATAACAA AGATGGCAGC 
TGTTGTGTTG AATACATCCC ATATGAGCCA GGGACATACA ACCTCAATGT CACCTATGGA 
GGCCAACCAA TCACTGGAAG CCCTTTCTCT GTACCTGTCC ATGATACTGT TGACCCAACT 
AAAGTGAAAT GTCAGGGCCA GGGGCTTGGA AACAACGTGC GTGCCAATAT CCCACAGGTC 
TTTTCTGTGG ATGCAAGCAA GGCTGGTATG GCGCCACTGC AGGTCAGAGT TCAGGGACCC 
AAAGGTGTTG TGGAGCCTGC TGAGGTGGTG GATAATGGTG ACCAGACTCA CACAGTCAGC 
TATGTGCCCA CAAGAGAGGG GCCATACTCC ATTAATGTTC TGTATGCTGA TGAGGAGATC 
CCACAGAGTC CTTATAAGGT GAAGGTTCTG CCCACTCATG ACGCAAGTAA AGTCCGTTGC 
AGCGGTCCTG GCCTGAATAC CACTGGTGTT CCTGCCAGTC TGCCCGTGGA GTTCACTATT 
GACGCCAAAG ATGCAGGAGA GGGACTGTTG GCCGTCCAGA TAACTGACCC TGATGGGAAG 
CCAAAGAAGG CTAACATTCG TGATAATCAG GACGGGACCT ACCTTGTGTC CTATGTGCCT 
GACATGACCG GCCGTTACAC CATTCTTATT AAGTATGGTG GTGATGAGAT CCCGTATTCT 
CCATATCGCA TTCGAGCCCT GCCTACTGGA GACGCCAGCA AGTGTACAGT CACAGGAGCT 
GGAGTTGGTC CGACTATTCA GATTGGAGAG CAGACTGTCA TCACTGTGGA TGCGAAGGCA 
GCTGGGAAGG GCAAGGTGAC CTGTACCGTG TGCACACCTG ATGGAGGCGA GGTGGATGTG 
GACGTCGTGG AAAATGAGGA TGGAACGTTT GACATCTTTT ACACTGCACC GCAGCCTGGG 
AAATACGTGA TCTGTGTGCG CTTCGGTGGA GAGCACATCC CTAACAGCCC CTTCCAAGTC 
ACGGCTTTGG AAGGTGACTC TCCTGACCAA CTAATGCAGC AGAGCCAGAA CCTGCAGTAC 
GCCTACGCGC CCAACATGGG CCAGACATGG GCTACAGATG GGCAATTGGG TATGAATGGA 
CTGGATGTAG CTGGACTGAG ACCCTTTGAC TTGGTCATTC CTTTCACCAT TCAGAAAGGA 
GAAATTACAG GTGATGTACG AATGCCCTCT GGAAAAGTGG CCAAGCCTGA TATTACAGAC 
AACAAGGATG GCACAGTCAC AGTGAAATAT GCCCCAACTG AGGCGGGCCT GCATGAGATG 
GACATTAAAT ATGATGGAAT ACACATTCCA GGAAGTCCAT TGCAATTCTA CGTGGATTAT 
GTCAACAGCG GTCATGTGAC TGCATATGGT CCTGGTCTAA TTCATGGCAT GGTCAATAAG 
CCTGCAGTCT TCACAGTCAA CACAAAGGAT GCTGGAGAGG GTGGTCTGTC TCTGGCCATC 
GAGGGGCCAT CAAAGGCAGA TATCAGCTGC ACTGATAATC AGGACGGTAC CTGCACTGTT 
TCATACCTCC CTGTTCTTCC CGGAGACTAC AATATCATTG TTAAATACAA TGACAAGAAC 
ATCCCCGGCA GCCCTTTCAT GTCCAAAATC ACAGGTGACG ACTCGATGCG CATGTCTCAC 
CTGAAGGTGG GCTCAGCTGC TGACATCCCT CTGGACATTG GTGAGCTTGA CCTCAGTCAG 
CTGACAGCCA CACTCACCAC CCCATCCGGC CGAGAGGAGC CTTGCCTGCT CAAGATGCTC 
AGAAATGGGC ATGTGGGAAT TTCCTTTGTG CCCAAGGAAA TTGGCGAGCA TCTTGTTAAC 
ATTAAGAAAA ATGGACGCCA TATTCCTAGT AGCCCCATTT CTGTCATGAT CAACCAATCA 
GAGATCGGTG ATGCCAGCCG TGTACGAGTA ACTGGTCAAG GCCTGAGTGA AGCTCGCACC 
TTTGAGCCCG CAGAATTCAT CATTGACACT AGAGATGCAG GATATGGTGG ACTCAGTTTG 
TCTATTGAGG GACCTAGTAA AGTGGATATC AACACTGAAG ATCAGGAAGA TGGCACCTGC 
AAAGTGACAT ATTGCCCCAC AGAGCCTGGA AACTACATAA TTAACATCAA ATTTGCAGAC 
CAGCATGTGC CAGGAAGTGC TTTCACTGTG AAAGTGACAG GCGAGGGTCG GATGAAGGAG 
AGCATCACCC GCAAAAGAAG AGCTGCTTCT GTGGCCAATG TTGGCAGCCA GTGCGACCTC 
AGTCTAAAGA TCCCTGAGAT CAGCATTGCA GACATGACCG CTCAGGTGAC CAGCCCATCT 
GGCAAAGTCC ACAAGGCAGA GATAATGGAG GGAGAAAACA ACACCTACTG CATCCGATTC 
GTGCCCACAG AGATGGGTGT GCACACAGTA AGCGTGAAGT ATCAAGGTCA GCATGTGCCT 
GGATCCCCCT TCCAGTTCAC GGTGGGACCT CTTGGAGAAG GAGGAGCTCA TAAAGTCAGA 
GCTGGAGGCC CAGGATTAGA GAGGGCAGAA GCTGGTGTAC CAGCGGAGTT CAGCATTTGG 
ACTCGTGAAG CAGGAGCTGG TGGTCTGTCC ATCGCAGTGG AAGGACCCAG CAAAGCAGAG 
ATTGCTTTTG AAGACCGTAA AGATGGATCC AGTGGTGTCT CTTACATCGT CCAGGAGCCT 
GGCGATTATG AAGTATCCAT CAAGTTCAAT GACGAGCACA TCCCTGACAG TCCGTTTGTG 
GTGCCTGTGG CCTCGCCATC TGACGATGCC CGCCGTCTCA CTGTTGCCAG TCTTCAGGAG 
TCCGGGTTAA AGGTGAACCA GCCGGCCTCT TTTGCTGTGA GCCTAAATGG GGCGAAAGGA 
GTCATCGATG CCAAAGTTCA CAGTCCATCA GGAGCACTTG AAGAGTGCTG CGTCACCGAG 
ATCGATGAAG ATAAATATGC TGTGAGATTC ATTCCCAGGG AGAATGGATT GTACCTGATA 
GATGTGAAAT TTAATGGTTC TCACATCCCT GGAAGCCCCT TCAAGATCCG TGTTGGTGAG 
ACGGGTCAGG CTGGAGATCC AGGAATGGTG TCTGCTTATG GAGCTGGTCT GGAAGGAGGC 
ACTACTGGAT CGCCTTGTGA GTTCATTGTA AACACCAGTT CAGCTGGCCC TGGAGCTTTG 
GCGGTGACTA TCGACGGGCC GTCCAAGGTG AAGATGGACT GTCAGGAATG CCCAGAGGGC 
TACAAAGTCA CCTACACACC TATGGCCCCT GGAAACTACC TAATTTCTAT TAAATACGGT 
GGACCGTACC ATATTGTTGG TAGTCCATTC AAAGCTAAAA TTACTGGCTC CCGATTGGTC 
AACAGCCACA GCATGCATGA GACTTCATCA GTACTGGTTG ACCCTGTGAC CCGCAGCATG 
ACGACCAGCC AGCAGGCAGC GCCAGGCTGG GCCAGCTCTG ATGCCAGCCG CGTAGTGGCC 
AAAGGTTTGG GGCTCAACAA GGGCTTCATA GGACAGAAGA ACACTTTCAG TGTGGACTGT 
AGTAAAGCAG GGAGGAACAT GCTCCTGGTC GGAGTGGATG GACCCAAAGT GCCCTGTGAG 
GAGATCCTGG TGAAACACCT GGGCAACCGC CTCTACAACG TTTCCTACCA GCTGAAGGAG 
AAAGGAGAGT ACATTCTGGT GGTCAAATGG GGTGATGAGC ACATCCCGGG CAGCCCGTAT 
CACATCACTG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

flna ( XM_009296771.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295046.1 Danio rerio filamin A, alpha (actin binding protein 280) (flna), transcript variant X2, mRNA.

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CloneID	ODa43842
Clone ID Related Accession (Same CDS sequence)	XM_021474524.1 , XM_009296771.2 , XM_009296771.3
Accession Version	XM_009296771.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	filamin-A isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009296771.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCAAC ACACACGCCC CCACCAGTCG AGCGCCGCCG CTCCCCTATC CAACGCTTCT 
CTCTCCCCGG ACAAGGACGC CGACATGCCC GCTACAGAGA AAGATCTCGC CGAGGACGCG 
CCATGGAAGA AAATCCAGCA AAATACATTC ACGCGTTGGT GCAACGAGCA TCTGAAATGT 
GTCAACAAGC GCATAGCGAA CCTGCAGACG GACCTGAGCG ACGGGCTCCG GCTGATCGCG 
CTTCTGGAGG TGCTCAGCCA GAAGAAGATG TTCCGCAAGT ACAACCAGCG CCCTACCTTC 
AGACAGATGC AGCTGGAGAA CGTCTCCGTG GCGCTGGAGT TCCTGGACAT TGAAAACATC 
AAGCTGGTCT CCATTGACAG TAAGGCCATA GTGGATGGTA ACCTGAAGCT GATCCTGGGT 
CTGATATGGA CTCTTATTCT GCATTATTCC ATCTCCATGC CCATGTGGGA CGAGGAAGAG 
GAGAGCGAGG AAAGCAAGCA GAAGACGCCC AAGCAGAGGC TCCTCGGATG GATCCAGAAC 
AAGCTGCCCG AGCTGCCAAT CACCAACTTC AGCCGCGATT GGCAGTCAGG GAAAGCCCTT 
GGCGCTCTGG TGGACAGTTG TGCTCCAGGT CTGTGTCCAG ACTGGGATTC CTGGGACCAG 
ACCAAGCCTG TGGACAATGC CAGAGAGGCC ATGCAGCAGG CTGATGACTG GCTCGGTGTT 
CCTCAGGTCA TCACACCAGA AGAAATCGTT GATCCCAATG TGGACGAGCA CTCCGTTATG 
ACTTACCTGT CCCAGTTTCC CAAAGCCAAA CTCAAGCCTG GTGCCCCTCT GAGGCCCAAA 
CTCAATCCCA AAAAGGCCCG TGCTTATGGA CCAGGTATTG AGCCAACAGG TAATGTTGTG 
ATGAAGAAGG CTGTGTTCAC TGTTGAGACC ATCAGCGCTG GTCAGGGTGA GGTACTGGTT 
TATGTCGAGG ACCCCGCTGG ACACCGCGAG GAGGCCAAAG TCACAGCCAA CAATGACAAG 
AACCGCACCT ACTCAGTATT CTATGTGCCA AAAGTCACTG GACAACACAA GGTGACAGTG 
CTGTTTGCAG GGCAACACAT CTCTAAGAGC CCGTTTGAGG TGGATGTGGG AATGGCTCAG 
GGAGACTCCA GCAAGGTCAC TGCCCAGGGC CCAGGACTCG AGCCTGCAGG CAACATTGCC 
AACAAAACCA CATACTTTGA TGTCTACACA GCTGGTGCTG GAGTAGGAGA GGTGGAGGTG 
GTCATCATGG ACCCCAGTGG AAAGAAGGAC ACGGTTGAGT GCAGTGTCGA GGATAAAGGC 
AACAGCAGCT ACCGATGCAC CTACAAGCCC ACTCAGGAGG GACAGCACAT CATTTACATC 
ACCTTTGCCG GGGGACAGAT CTCCAAGAGC CCTTACACTG TCAATGTGGG AGAGGCCTGT 
AATCCAAGCC TGTGTACAGC CAAGGGCCGC GGTCTGCAGC CTAAAGGCCT GAGGGTCAAG 
GAGACCGCCG AGTTTAAGGT TTACACCAAA GGAGCAGGAA CCGGAGACCT CAAAGTTACC 
ATCAAAGGGC CCAAGGGTCT CGAGGAGCCC TGTAAGAAGA AGGATTTGGG AGACGGCGTG 
TACAGCTTTG ATTATTACCC CTCCACACCT GGAAATTACA TCATCACCAT CACATGGGGC 
GGTCAACACA TCCCACGCAG CCCGTTTGAG GTGAAGATCG GGTCTGAAGC AGGACCGCAG 
CAGGTCAGGG CGTGGGGTCC AGGACTAGAG AGTGGCGTTG TGGGCAAATC TGCTGACTTT 
GTTGTGGAAG CTGTTGGTGA TGATGTGGGC ACTTTAGGTT TCTCTGTTGA AGGCCCTTCC 
CCGGCTAAGA TTGAATGTGA TGATAAGGGA GATGGTTCAT GTGACGTGAG GTACTGGCCC 
ACCGAACCGG GTGAATATGC AGTTCATGTG CTCTGTAAGA ATGAGGACAT CCAGCTCAGC 
CCTTTCATGG CTGAAATCAT GCCCGCCCCA GGAAAAGACT TTTATCCTGA CAAGGTGAAG 
GCTTATGGCC CCGGTCTGCA GAGCTCAGGC CTGTCAGTTG GCAAACCAGC CGAGTTTACC 
GTGGATGCCA AACTGGGTGG CAAAGCTCCT CTGAAGATTC AAGCTCAGGA CCGTGATGGA 
AACCCAGTCG ATGTGCAGGT GAAGGACAAT GGAAATGGGA CATACAGCTG CAGCTACACC 
CCTCGCAAAC CAGTCAAACA CACTGTGATG GTGTCCTGGG GCGGCGTCAA CATCCCAGAG 
AGCCCGTTCA GAATGAACAT TGGGGCAGGG TGTCATCCCA ATAAGGTGAA AGTATCTGGA 
CCTGGAGTGG CCAAGACAGG CCTCAAAGCA TATGATCCAA CATACTTTAC TGTGGATTGT 
GCTGAGGCTG GACAGGGCGA CATCAGCATA GGCATCAAAT GTGCTGCAGG TGTGGTGGGA 
CCTGCTGAGG CTGACATAGA CTTCGACATC ATTAGAAATG ACAACGACAC ATTTACTGTT 
AAATACACTC CTCCTGGAGC GGGCAGCTAC ACCATCATGG TGCTGTTTGC TGATCAGACC 
ATTCCCATGA CACCCATCAG AATTAAGGTT GATCCCTCTC ATGATGCAAG CAAGGTTAAA 
GCTGAGGGGC CTGGACTTAG CCGTTCTGGT GTGGAGCTGA ACAAACCCAC TCACTTCACT 
GTGAACACAA AGGCTGCAGG CAAGGCCAAG CTTGATGCTC AGTTCACTGG ACCCAACAAG 
GGAGATGCAG TCCGTGACTT CGACATAATC GACAACCATG ATGGCACTCA CACTGTCAAA 
TATACCCCTG TCCAACAGGG CAACATGGGT TTGAATGTCA CCTACGGTGG TGACCCTATT 
CCCAAGAGCC CATTTGCAGT AGCAATTGCC CCCTCTCTGG ACCTCAGTAA AGTTAAAGTG 
GCTGGTCTTG GAAGTAAAAT GACTGTTGGA AAGGATCAGG AGATCACAGT CAAATCTAAA 
GGTGCAGGCG GTCAGGGCAA AGTTGGCGCA AAGGTTACTG GTCCATCTGC TAAATCAGTG 
CCCTGTAAAG TTGAACCAGG CCTGAGCCCA GAAACCAGCC AAGTTCGTTT TATCCCAAGA 
GATAAGGGAC CCTATGAGGT GGAACTGACA TATGATGGCG CCCCCATCCC TGGCAGTCCA 
TTCCCCATCG AAGCCATTGC ACCTACTGAC CCATCCAAGG TGCGATGCTC TGGGCCAGGA 
CTGGAGCGGG CGAAGGTTGG CGAGCCTGGA CAGTTTGTGG TGGATTGCAC TAATGCTGGC 
CCTGCTGAGT TGACCATCGA GATCATCTCT GACAATGGCA CTGAGGCTGA AGTCCACATT 
CAAGACAACG GCGACGGGAC CTACACCATC ACTTACGTCC CTCTCTACCC TGGAGCCTAT 
ACCCTCACCA TCCGCTACGG GGACCAGGAT GTGCCAAACT TCCCAGCAAG ACTCCATGTG 
GAGCCTGCTG TGGAGACCAG TGGAGTGAAA GTGTTCGGAC CCGGAGTGGA GGGCAAAGGT 
GTTTTCAGGG AAGCCACTAC AGATTTCACT GTGGATGCCC GTGCGCTCAC TAAAACCGGA 
GGCAATCACA TCAAAACCTG CATCAACAAC CCATCGGGCA ACCGCACTGA AGCACTGATC 
CGAGATCTGG GAGACGGCAC ATATCAAGTG GAGTACACGC CTTATGAGGA GGGTACACAC 
AGTGTTGAGG TTGCATATGA CAGCTCCCCG GTTCCCAAAA GCCCCTTCCG TGTGCCAGTG 
ACCGAGGGCT GTGACCCGGC GCGTGTGCGT GTGCATGGTC CAGGACTTCA GTCTGGCATC 
ACCAACAAAC CTAACAAATT TACCGTGGAG ACCCGTGGGG CTGGTACTGG TGGACTGGGG 
CTGGCTATGG AAGGACCTTC TGAGGCCAAA ATGTCCTGCA CCGATAACAA AGATGGCAGC 
TGTTGTGTTG AATACATCCC ATATGAGCCA GGGACATACA ACCTCAATGT CACCTATGGA 
GGCCAACCAA TCACTGGAAG CCCTTTCTCT GTACCTGTCC ATGATACTGT TGACCCAACT 
AAAGTGAAAT GTCAGGGCCA GGGGCTTGGA AACAACGTGC GTGCCAATAT CCCACAGGTC 
TTTTCTGTGG ATGCAAGCAA GGCTGGTATG GCGCCACTGC AGGTCAGAGT TCAGGGACCC 
AAAGGTGTTG TGGAGCCTGC TGAGGTGGTG GATAATGGTG ACCAGACTCA CACAGTCAGC 
TATGTGCCCA CAAGAGAGGG GCCATACTCC ATTAATGTTC TGTATGCTGA TGAGGAGATC 
CCACAGAGTC CTTATAAGGT GAAGGTTCTG CCCACTCATG ACGCAAGTAA AGTCCGTTGC 
AGCGGTCCTG GCCTGAATAC CACTGGTGTT CCTGCCAGTC TGCCCGTGGA GTTCACTATT 
GACGCCAAAG ATGCAGGAGA GGGACTGTTG GCCGTCCAGA TAACTGACCC TGATGGGAAG 
CCAAAGAAGG CTAACATTCG TGATAATCAG GACGGGACCT ACCTTGTGTC CTATGTGCCT 
GACATGACCG GCCGTTACAC CATTCTTATT AAGTATGGTG GTGATGAGAT CCCGTATTCT 
CCATATCGCA TTCGAGCCCT GCCTACTGGA GACGCCAGCA AGTGTACAGT CACAGGAGCT 
GGAGTTGGTC CGACTATTCA GATTGGAGAG CAGACTGTCA TCACTGTGGA TGCGAAGGCA 
GCTGGGAAGG GCAAGGTGAC CTGTACCGTG TGCACACCTG ATGGAGGCGA GGTGGATGTG 
GACGTCGTGG AAAATGAGGA TGGAACGTTT GACATCTTTT ACACTGCACC GCAGCCTGGG 
AAATACGTGA TCTGTGTGCG CTTCGGTGGA GAGCACATCC CTAACAGCCC CTTCCAAGTC 
ACGGCTTTGG AAGGTGACTC TCCTGACCAA CTAATGCAGC AGAGCCAGAA CCTGCAGTAC 
GCCTACGCGC CCAACATGGG CCAGACATGG GCTACAGATG GGCAATTGGG TATGAATGGA 
CTGGATGTAG CTGGACTGAG ACCCTTTGAC TTGGTCATTC CTTTCACCAT TCAGAAAGGA 
GAAATTACAG GTGATGTACG AATGCCCTCT GGAAAAGTGG CCAAGCCTGA TATTACAGAC 
AACAAGGATG GCACAGTCAC AGTGAAATAT GCCCCAACTG AGGCGGGCCT GCATGAGATG 
GACATTAAAT ATGATGGAAT ACACATTCCA GGAAGTCCAT TGCAATTCTA CGTGGATTAT 
GTCAACAGCG GTCATGTGAC TGCATATGGT CCTGGTCTAA TTCATGGCAT GGTCAATAAG 
CCTGCAGTCT TCACAGTCAA CACAAAGGAT GCTGGAGAGG GTGGTCTGTC TCTGGCCATC 
GAGGGGCCAT CAAAGGCAGA TATCAGCTGC ACTGATAATC AGGACGGTAC CTGCACTGTT 
TCATACCTCC CTGTTCTTCC CGGAGACTAC AATATCATTG TTAAATACAA TGACAAGAAC 
ATCCCCGGCA GCCCTTTCAT GTCCAAAATC ACAGGTGACG ACTCGATGCG CATGTCTCAC 
CTGAAGGTGG GCTCAGCTGC TGACATCCCT CTGGACATTG GTGAGCTTGA CCTCAGTCAG 
CTGACAGCCA CACTCACCAC CCCATCCGGC CGAGAGGAGC CTTGCCTGCT CAAGATGCTC 
AGAAATGGGC ATGTGGGAAT TTCCTTTGTG CCCAAGGAAA TTGGCGAGCA TCTTGTTAAC 
ATTAAGAAAA ATGGACGCCA TATTCCTAGT AGCCCCATTT CTGTCATGAT CAACCAATCA 
GAGATCGGTG ATGCCAGCCG TGTACGAGTA ACTGGTCAAG GCCTGAGTGA AGCTCGCACC 
TTTGAGCCCG CAGAATTCAT CATTGACACT AGAGATGCAG GATATGGTGG ACTCAGTTTG 
TCTATTGAGG GACCTAGTAA AGTGGATATC AACACTGAAG ATCAGGAAGA TGGCACCTGC 
AAAGTGACAT ATTGCCCCAC AGAGCCTGGA AACTACATAA TTAACATCAA ATTTGCAGAC 
CAGCATGTGC CAGGAAGTGC TTTCACTGTG AAAGTGACAG GCGAGGGTCG GATGAAGGAG 
AGCATCACCC GCAAAAGAAG AGCTGCTTCT GTGGCCAATG TTGGCAGCCA GTGCGACCTC 
AGTCTAAAGA TCCCTGAGAT CAGCATTGCA GACATGACCG CTCAGGTGAC CAGCCCATCT 
GGCAAAGTCC ACAAGGCAGA GATAATGGAG GGAGAAAACA ACACCTACTG CATCCGATTC 
GTGCCCACAG AGATGGGTGT GCACACAGTA AGCGTGAAGT ATCAAGGTCA GCATGTGCCT 
GGATCCCCCT TCCAGTTCAC GGTGGGACCT CTTGGAGAAG GAGGAGCTCA TAAAGTCAGA 
GCTGGAGGCC CAGGATTAGA GAGGGCAGAA GCTGGTGTAC CAGCGGAGTT CAGCATTTGG 
ACTCGTGAAG CAGGAGCTGG TGGTCTGTCC ATCGCAGTGG AAGGACCCAG CAAAGCAGAG 
ATTGCTTTTG AAGACCGTAA AGATGGATCC AGTGGTGTCT CTTACATCGT CCAGGAGCCT 
GGCGATTATG AAGTATCCAT CAAGTTCAAT GACGAGCACA TCCCTGACAG TCCGTTTGTG 
GTGCCTGTGG CCTCGCCATC TGACGATGCC CGCCGTCTCA CTGTTGCCAG TCTTCAGGAG 
TCCGGGTTAA AGGTGAACCA GCCGGCCTCT TTTGCTGTGA GCCTAAATGG GGCGAAAGGA 
GTCATCGATG CCAAAGTTCA CAGTCCATCA GGAGCACTTG AAGAGTGCTG CGTCACCGAG 
ATCGATGAAG ATAAATATGC TGTGAGATTC ATTCCCAGGG AGAATGGATT GTACCTGATA 
GATGTGAAAT TTAATGGTTC TCACATCCCT GGAAGCCCCT TCAAGATCCG TGTTGGTGAG 
ACGGGTCAGG CTGGAGATCC AGGAATGGTG TCTGCTTATG GAGCTGGTCT GGAAGGAGGC 
ACTACTGGAT CGCCTTGTGA GTTCATTGTA AACACCAGTT CAGCTGGCCC TGGAGCTTTG 
GCGGTGACTA TCGACGGGCC GTCCAAGGTG AAGATGGACT GTCAGGAATG CCCAGAGGGC 
TACAAAGTCA CCTACACACC TATGGCCCCT GGAAACTACC TAATTTCTAT TAAATACGGT 
GGACCGTACC ATATTGTTGG TAGTCCATTC AAAGCTAAAA TTACTGGCTC CCGATTGGTC 
AACAGCCACA GCATGCATGA GACTTCATCA GTACTGGTTG ACCCTGTGAC CCGCAGCATG 
ACGACCAGCC AGCAGGCAGC GCCAGGCTGG GCCAGCTCTG ATGCCAGCCG CGTAGTGGCC 
AAAGGTTTGG GGCTCAACAA GGGCTTCATA GGACAGAAGA ACACTTTCAG TGTGGACTGT 
AGTAAAGCAG GGAGGAACAT GCTCCTGGTC GGAGTGGATG GACCCAAAGT GCCCTGTGAG 
GAGATCCTGG TGAAACACCT GGGCAACCGC CTCTACAACG TTTCCTACCA GCTGAAGGAG 
AAAGGAGAGT ACATTCTGGT GGTCAAATGG GGTGATGAGC ACATCCCGGG CAGCCCGTAT 
CACATCACTG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295046.1
CDS	370..8004
Translation	MSQHTRPHQSSAAAPLSNASLSPDKDADMPATEKDLAEDAPWKKIQQNTFTRWCNEHLKCVNKRIANLQTDLSDGLRLIALLEVLSQKKMFRKYNQRPTFRQMQLENVSVALEFLDIENIKLVSIDSKAIVDGNLKLILGLIWTLILHYSISMPMWDEEEESEESKQKTPKQRLLGWIQNKLPELPITNFSRDWQSGKALGALVDSCAPGLCPDWDSWDQTKPVDNAREAMQQADDWLGVPQVITPEEIVDPNVDEHSVMTYLSQFPKAKLKPGAPLRPKLNPKKARAYGPGIEPTGNVVMKKAVFTVETISAGQGEVLVYVEDPAGHREEAKVTANNDKNRTYSVFYVPKVTGQHKVTVLFAGQHISKSPFEVDVGMAQGDSSKVTAQGPGLEPAGNIANKTTYFDVYTAGAGVGEVEVVIMDPSGKKDTVECSVEDKGNSSYRCTYKPTQEGQHIIYITFAGGQISKSPYTVNVGEACNPSLCTAKGRGLQPKGLRVKETAEFKVYTKGAGTGDLKVTIKGPKGLEEPCKKKDLGDGVYSFDYYPSTPGNYIITITWGGQHIPRSPFEVKIGSEAGPQQVRAWGPGLESGVVGKSADFVVEAVGDDVGTLGFSVEGPSPAKIECDDKGDGSCDVRYWPTEPGEYAVHVLCKNEDIQLSPFMAEIMPAPGKDFYPDKVKAYGPGLQSSGLSVGKPAEFTVDAKLGGKAPLKIQAQDRDGNPVDVQVKDNGNGTYSCSYTPRKPVKHTVMVSWGGVNIPESPFRMNIGAGCHPNKVKVSGPGVAKTGLKAYDPTYFTVDCAEAGQGDISIGIKCAAGVVGPAEADIDFDIIRNDNDTFTVKYTPPGAGSYTIMVLFADQTIPMTPIRIKVDPSHDASKVKAEGPGLSRSGVELNKPTHFTVNTKAAGKAKLDAQFTGPNKGDAVRDFDIIDNHDGTHTVKYTPVQQGNMGLNVTYGGDPIPKSPFAVAIAPSLDLSKVKVAGLGSKMTVGKDQEITVKSKGAGGQGKVGAKVTGPSAKSVPCKVEPGLSPETSQVRFIPRDKGPYEVELTYDGAPIPGSPFPIEAIAPTDPSKVRCSGPGLERAKVGEPGQFVVDCTNAGPAELTIEIISDNGTEAEVHIQDNGDGTYTITYVPLYPGAYTLTIRYGDQDVPNFPARLHVEPAVETSGVKVFGPGVEGKGVFREATTDFTVDARALTKTGGNHIKTCINNPSGNRTEALIRDLGDGTYQVEYTPYEEGTHSVEVAYDSSPVPKSPFRVPVTEGCDPARVRVHGPGLQSGITNKPNKFTVETRGAGTGGLGLAMEGPSEAKMSCTDNKDGSCCVEYIPYEPGTYNLNVTYGGQPITGSPFSVPVHDTVDPTKVKCQGQGLGNNVRANIPQVFSVDASKAGMAPLQVRVQGPKGVVEPAEVVDNGDQTHTVSYVPTREGPYSINVLYADEEIPQSPYKVKVLPTHDASKVRCSGPGLNTTGVPASLPVEFTIDAKDAGEGLLAVQITDPDGKPKKANIRDNQDGTYLVSYVPDMTGRYTILIKYGGDEIPYSPYRIRALPTGDASKCTVTGAGVGPTIQIGEQTVITVDAKAAGKGKVTCTVCTPDGGEVDVDVVENEDGTFDIFYTAPQPGKYVICVRFGGEHIPNSPFQVTALEGDSPDQLMQQSQNLQYAYAPNMGQTWATDGQLGMNGLDVAGLRPFDLVIPFTIQKGEITGDVRMPSGKVAKPDITDNKDGTVTVKYAPTEAGLHEMDIKYDGIHIPGSPLQFYVDYVNSGHVTAYGPGLIHGMVNKPAVFTVNTKDAGEGGLSLAIEGPSKADISCTDNQDGTCTVSYLPVLPGDYNIIVKYNDKNIPGSPFMSKITGDDSMRMSHLKVGSAADIPLDIGELDLSQLTATLTTPSGREEPCLLKMLRNGHVGISFVPKEIGEHLVNIKKNGRHIPSSPISVMINQSEIGDASRVRVTGQGLSEARTFEPAEFIIDTRDAGYGGLSLSIEGPSKVDINTEDQEDGTCKVTYCPTEPGNYIINIKFADQHVPGSAFTVKVTGEGRMKESITRKRRAASVANVGSQCDLSLKIPEISIADMTAQVTSPSGKVHKAEIMEGENNTYCIRFVPTEMGVHTVSVKYQGQHVPGSPFQFTVGPLGEGGAHKVRAGGPGLERAEAGVPAEFSIWTREAGAGGLSIAVEGPSKAEIAFEDRKDGSSGVSYIVQEPGDYEVSIKFNDEHIPDSPFVVPVASPSDDARRLTVASLQESGLKVNQPASFAVSLNGAKGVIDAKVHSPSGALEECCVTEIDEDKYAVRFIPRENGLYLIDVKFNGSHIPGSPFKIRVGETGQAGDPGMVSAYGAGLEGGTTGSPCEFIVNTSSAGPGALAVTIDGPSKVKMDCQECPEGYKVTYTPMAPGNYLISIKYGGPYHIVGSPFKAKITGSRLVNSHSMHETSSVLVDPVTRSMTTSQQAAPGWASSDASRVVAKGLGLNKGFIGQKNTFSVDCSKAGRNMLLVGVDGPKVPCEEILVKHLGNRLYNVSYQLKEKGEYILVVKWGDEHIPGSPYHITV

Target ORF information:

RefSeq Version	XM_009296771.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio filamin A, alpha (actin binding protein 280) (flna), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296771.2

ORF Insert Sequence:

ATGAGTCAAC ACACACGCCC CCACCAGTCG AGCGCCGCCG CTCCCCTATC CAACGCTTCT 
CTCTCCCCGG ACAAGGACGC CGACATGCCC GCTACAGAGA AAGATCTCGC CGAGGACGCG 
CCATGGAAGA AAATCCAGCA AAATACATTC ACGCGTTGGT GCAACGAGCA TCTGAAATGT 
GTCAACAAGC GCATAGCGAA CCTGCAGACG GACCTGAGCG ACGGGCTCCG GCTGATCGCG 
CTTCTGGAGG TGCTCAGCCA GAAGAAGATG TTCCGCAAGT ACAACCAGCG CCCTACCTTC 
AGACAGATGC AGCTGGAGAA CGTCTCCGTG GCGCTGGAGT TCCTGGACAT TGAAAACATC 
AAGCTGGTCT CCATTGACAG TAAGGCCATA GTGGATGGTA ACCTGAAGCT GATCCTGGGT 
CTGATATGGA CTCTTATTCT GCATTATTCC ATCTCCATGC CCATGTGGGA CGAGGAAGAG 
GAGAGCGAGG AAAGCAAGCA GAAGACGCCC AAGCAGAGGC TCCTCGGATG GATCCAGAAC 
AAGCTGCCCG AGCTGCCAAT CACCAACTTC AGCCGCGATT GGCAGTCAGG GAAAGCCCTT 
GGCGCTCTGG TGGACAGTTG TGCTCCAGGT CTGTGTCCAG ACTGGGATTC CTGGGACCAG 
ACCAAGCCTG TGGACAATGC CAGAGAGGCC ATGCAGCAGG CTGATGACTG GCTCGGTGTT 
CCTCAGGTCA TCACACCAGA AGAAATCGTT GATCCCAATG TGGACGAGCA CTCCGTTATG 
ACTTACCTGT CCCAGTTTCC CAAAGCCAAA CTCAAGCCTG GTGCCCCTCT GAGGCCCAAA 
CTCAATCCCA AAAAGGCCCG TGCTTATGGA CCAGGTATTG AGCCAACAGG TAATGTTGTG 
ATGAAGAAGG CTGTGTTCAC TGTTGAGACC ATCAGCGCTG GTCAGGGTGA GGTACTGGTT 
TATGTCGAGG ACCCCGCTGG ACACCGCGAG GAGGCCAAAG TCACAGCCAA CAATGACAAG 
AACCGCACCT ACTCAGTATT CTATGTGCCA AAAGTCACTG GACAACACAA GGTGACAGTG 
CTGTTTGCAG GGCAACACAT CTCTAAGAGC CCGTTTGAGG TGGATGTGGG AATGGCTCAG 
GGAGACTCCA GCAAGGTCAC TGCCCAGGGC CCAGGACTCG AGCCTGCAGG CAACATTGCC 
AACAAAACCA CATACTTTGA TGTCTACACA GCTGGTGCTG GAGTAGGAGA GGTGGAGGTG 
GTCATCATGG ACCCCAGTGG AAAGAAGGAC ACGGTTGAGT GCAGTGTCGA GGATAAAGGC 
AACAGCAGCT ACCGATGCAC CTACAAGCCC ACTCAGGAGG GACAGCACAT CATTTACATC 
ACCTTTGCCG GGGGACAGAT CTCCAAGAGC CCTTACACTG TCAATGTGGG AGAGGCCTGT 
AATCCAAGCC TGTGTACAGC CAAGGGCCGC GGTCTGCAGC CTAAAGGCCT GAGGGTCAAG 
GAGACCGCCG AGTTTAAGGT TTACACCAAA GGAGCAGGAA CCGGAGACCT CAAAGTTACC 
ATCAAAGGGC CCAAGGGTCT CGAGGAGCCC TGTAAGAAGA AGGATTTGGG AGACGGCGTG 
TACAGCTTTG ATTATTACCC CTCCACACCT GGAAATTACA TCATCACCAT CACATGGGGC 
GGTCAACACA TCCCACGCAG CCCGTTTGAG GTGAAGATCG GGTCTGAAGC AGGACCGCAG 
CAGGTCAGGG CGTGGGGTCC AGGACTAGAG AGTGGCGTTG TGGGCAAATC TGCTGACTTT 
GTTGTGGAAG CTGTTGGTGA TGATGTGGGC ACTTTAGGTT TCTCTGTTGA AGGCCCTTCC 
CCGGCTAAGA TTGAATGTGA TGATAAGGGA GATGGTTCAT GTGACGTGAG GTACTGGCCC 
ACCGAACCGG GTGAATATGC AGTTCATGTG CTCTGTAAGA ATGAGGACAT CCAGCTCAGC 
CCTTTCATGG CTGAAATCAT GCCCGCCCCA GGAAAAGACT TTTATCCTGA CAAGGTGAAG 
GCTTATGGCC CCGGTCTGCA GAGCTCAGGC CTGTCAGTTG GCAAACCAGC CGAGTTTACC 
GTGGATGCCA AACTGGGTGG CAAAGCTCCT CTGAAGATTC AAGCTCAGGA CCGTGATGGA 
AACCCAGTCG ATGTGCAGGT GAAGGACAAT GGAAATGGGA CATACAGCTG CAGCTACACC 
CCTCGCAAAC CAGTCAAACA CACTGTGATG GTGTCCTGGG GCGGCGTCAA CATCCCAGAG 
AGCCCGTTCA GAATGAACAT TGGGGCAGGG TGTCATCCCA ATAAGGTGAA AGTATCTGGA 
CCTGGAGTGG CCAAGACAGG CCTCAAAGCA TATGATCCAA CATACTTTAC TGTGGATTGT 
GCTGAGGCTG GACAGGGCGA CATCAGCATA GGCATCAAAT GTGCTGCAGG TGTGGTGGGA 
CCTGCTGAGG CTGACATAGA CTTCGACATC ATTAGAAATG ACAACGACAC ATTTACTGTT 
AAATACACTC CTCCTGGAGC GGGCAGCTAC ACCATCATGG TGCTGTTTGC TGATCAGACC 
ATTCCCATGA CACCCATCAG AATTAAGGTT GATCCCTCTC ATGATGCAAG CAAGGTTAAA 
GCTGAGGGGC CTGGACTTAG CCGTTCTGGT GTGGAGCTGA ACAAACCCAC TCACTTCACT 
GTGAACACAA AGGCTGCAGG CAAGGCCAAG CTTGATGCTC AGTTCACTGG ACCCAACAAG 
GGAGATGCAG TCCGTGACTT CGACATAATC GACAACCATG ATGGCACTCA CACTGTCAAA 
TATACCCCTG TCCAACAGGG CAACATGGGT TTGAATGTCA CCTACGGTGG TGACCCTATT 
CCCAAGAGCC CATTTGCAGT AGCAATTGCC CCCTCTCTGG ACCTCAGTAA AGTTAAAGTG 
GCTGGTCTTG GAAGTAAAAT GACTGTTGGA AAGGATCAGG AGATCACAGT CAAATCTAAA 
GGTGCAGGCG GTCAGGGCAA AGTTGGCGCA AAGGTTACTG GTCCATCTGC TAAATCAGTG 
CCCTGTAAAG TTGAACCAGG CCTGAGCCCA GAAACCAGCC AAGTTCGTTT TATCCCAAGA 
GATAAGGGAC CCTATGAGGT GGAACTGACA TATGATGGCG CCCCCATCCC TGGCAGTCCA 
TTCCCCATCG AAGCCATTGC ACCTACTGAC CCATCCAAGG TGCGATGCTC TGGGCCAGGA 
CTGGAGCGGG CGAAGGTTGG CGAGCCTGGA CAGTTTGTGG TGGATTGCAC TAATGCTGGC 
CCTGCTGAGT TGACCATCGA GATCATCTCT GACAATGGCA CTGAGGCTGA AGTCCACATT 
CAAGACAACG GCGACGGGAC CTACACCATC ACTTACGTCC CTCTCTACCC TGGAGCCTAT 
ACCCTCACCA TCCGCTACGG GGACCAGGAT GTGCCAAACT TCCCAGCAAG ACTCCATGTG 
GAGCCTGCTG TGGAGACCAG TGGAGTGAAA GTGTTCGGAC CCGGAGTGGA GGGCAAAGGT 
GTTTTCAGGG AAGCCACTAC AGATTTCACT GTGGATGCCC GTGCGCTCAC TAAAACCGGA 
GGCAATCACA TCAAAACCTG CATCAACAAC CCATCGGGCA ACCGCACTGA AGCACTGATC 
CGAGATCTGG GAGACGGCAC ATATCAAGTG GAGTACACGC CTTATGAGGA GGGTACACAC 
AGTGTTGAGG TTGCATATGA CAGCTCCCCG GTTCCCAAAA GCCCCTTCCG TGTGCCAGTG 
ACCGAGGGCT GTGACCCGGC GCGTGTGCGT GTGCATGGTC CAGGACTTCA GTCTGGCATC 
ACCAACAAAC CTAACAAATT TACCGTGGAG ACCCGTGGGG CTGGTACTGG TGGACTGGGG 
CTGGCTATGG AAGGACCTTC TGAGGCCAAA ATGTCCTGCA CCGATAACAA AGATGGCAGC 
TGTTGTGTTG AATACATCCC ATATGAGCCA GGGACATACA ACCTCAATGT CACCTATGGA 
GGCCAACCAA TCACTGGAAG CCCTTTCTCT GTACCTGTCC ATGATACTGT TGACCCAACT 
AAAGTGAAAT GTCAGGGCCA GGGGCTTGGA AACAACGTGC GTGCCAATAT CCCACAGGTC 
TTTTCTGTGG ATGCAAGCAA GGCTGGTATG GCGCCACTGC AGGTCAGAGT TCAGGGACCC 
AAAGGTGTTG TGGAGCCTGC TGAGGTGGTG GATAATGGTG ACCAGACTCA CACAGTCAGC 
TATGTGCCCA CAAGAGAGGG GCCATACTCC ATTAATGTTC TGTATGCTGA TGAGGAGATC 
CCACAGAGTC CTTATAAGGT GAAGGTTCTG CCCACTCATG ACGCAAGTAA AGTCCGTTGC 
AGCGGTCCTG GCCTGAATAC CACTGGTGTT CCTGCCAGTC TGCCCGTGGA GTTCACTATT 
GACGCCAAAG ATGCAGGAGA GGGACTGTTG GCCGTCCAGA TAACTGACCC TGATGGGAAG 
CCAAAGAAGG CTAACATTCG TGATAATCAG GACGGGACCT ACCTTGTGTC CTATGTGCCT 
GACATGACCG GCCGTTACAC CATTCTTATT AAGTATGGTG GTGATGAGAT CCCGTATTCT 
CCATATCGCA TTCGAGCCCT GCCTACTGGA GACGCCAGCA AGTGTACAGT CACAGGAGCT 
GGAGTTGGTC CGACTATTCA GATTGGAGAG CAGACTGTCA TCACTGTGGA TGCGAAGGCA 
GCTGGGAAGG GCAAGGTGAC CTGTACCGTG TGCACACCTG ATGGAGGCGA GGTGGATGTG 
GACGTCGTGG AAAATGAGGA TGGAACGTTT GACATCTTTT ACACTGCACC GCAGCCTGGG 
AAATACGTGA TCTGTGTGCG CTTCGGTGGA GAGCACATCC CTAACAGCCC CTTCCAAGTC 
ACGGCTTTGG AAGGTGACTC TCCTGACCAA CTAATGCAGC AGAGCCAGAA CCTGCAGTAC 
GCCTACGCGC CCAACATGGG CCAGACATGG GCTACAGATG GGCAATTGGG TATGAATGGA 
CTGGATGTAG CTGGACTGAG ACCCTTTGAC TTGGTCATTC CTTTCACCAT TCAGAAAGGA 
GAAATTACAG GTGATGTACG AATGCCCTCT GGAAAAGTGG CCAAGCCTGA TATTACAGAC 
AACAAGGATG GCACAGTCAC AGTGAAATAT GCCCCAACTG AGGCGGGCCT GCATGAGATG 
GACATTAAAT ATGATGGAAT ACACATTCCA GGAAGTCCAT TGCAATTCTA CGTGGATTAT 
GTCAACAGCG GTCATGTGAC TGCATATGGT CCTGGTCTAA TTCATGGCAT GGTCAATAAG 
CCTGCAGTCT TCACAGTCAA CACAAAGGAT GCTGGAGAGG GTGGTCTGTC TCTGGCCATC 
GAGGGGCCAT CAAAGGCAGA TATCAGCTGC ACTGATAATC AGGACGGTAC CTGCACTGTT 
TCATACCTCC CTGTTCTTCC CGGAGACTAC AATATCATTG TTAAATACAA TGACAAGAAC 
ATCCCCGGCA GCCCTTTCAT GTCCAAAATC ACAGGTGACG ACTCGATGCG CATGTCTCAC 
CTGAAGGTGG GCTCAGCTGC TGACATCCCT CTGGACATTG GTGAGCTTGA CCTCAGTCAG 
CTGACAGCCA CACTCACCAC CCCATCCGGC CGAGAGGAGC CTTGCCTGCT CAAGATGCTC 
AGAAATGGGC ATGTGGGAAT TTCCTTTGTG CCCAAGGAAA TTGGCGAGCA TCTTGTTAAC 
ATTAAGAAAA ATGGACGCCA TATTCCTAGT AGCCCCATTT CTGTCATGAT CAACCAATCA 
GAGATCGGTG ATGCCAGCCG TGTACGAGTA ACTGGTCAAG GCCTGAGTGA AGCTCGCACC 
TTTGAGCCCG CAGAATTCAT CATTGACACT AGAGATGCAG GATATGGTGG ACTCAGTTTG 
TCTATTGAGG GACCTAGTAA AGTGGATATC AACACTGAAG ATCAGGAAGA TGGCACCTGC 
AAAGTGACAT ATTGCCCCAC AGAGCCTGGA AACTACATAA TTAACATCAA ATTTGCAGAC 
CAGCATGTGC CAGGAAGTGC TTTCACTGTG AAAGTGACAG GCGAGGGTCG GATGAAGGAG 
AGCATCACCC GCAAAAGAAG AGCTGCTTCT GTGGCCAATG TTGGCAGCCA GTGCGACCTC 
AGTCTAAAGA TCCCTGAGAT CAGCATTGCA GACATGACCG CTCAGGTGAC CAGCCCATCT 
GGCAAAGTCC ACAAGGCAGA GATAATGGAG GGAGAAAACA ACACCTACTG CATCCGATTC 
GTGCCCACAG AGATGGGTGT GCACACAGTA AGCGTGAAGT ATCAAGGTCA GCATGTGCCT 
GGATCCCCCT TCCAGTTCAC GGTGGGACCT CTTGGAGAAG GAGGAGCTCA TAAAGTCAGA 
GCTGGAGGCC CAGGATTAGA GAGGGCAGAA GCTGGTGTAC CAGCGGAGTT CAGCATTTGG 
ACTCGTGAAG CAGGAGCTGG TGGTCTGTCC ATCGCAGTGG AAGGACCCAG CAAAGCAGAG 
ATTGCTTTTG AAGACCGTAA AGATGGATCC AGTGGTGTCT CTTACATCGT CCAGGAGCCT 
GGCGATTATG AAGTATCCAT CAAGTTCAAT GACGAGCACA TCCCTGACAG TCCGTTTGTG 
GTGCCTGTGG CCTCGCCATC TGACGATGCC CGCCGTCTCA CTGTTGCCAG TCTTCAGGAG 
TCCGGGTTAA AGGTGAACCA GCCGGCCTCT TTTGCTGTGA GCCTAAATGG GGCGAAAGGA 
GTCATCGATG CCAAAGTTCA CAGTCCATCA GGAGCACTTG AAGAGTGCTG CGTCACCGAG 
ATCGATGAAG ATAAATATGC TGTGAGATTC ATTCCCAGGG AGAATGGATT GTACCTGATA 
GATGTGAAAT TTAATGGTTC TCACATCCCT GGAAGCCCCT TCAAGATCCG TGTTGGTGAG 
ACGGGTCAGG CTGGAGATCC AGGAATGGTG TCTGCTTATG GAGCTGGTCT GGAAGGAGGC 
ACTACTGGAT CGCCTTGTGA GTTCATTGTA AACACCAGTT CAGCTGGCCC TGGAGCTTTG 
GCGGTGACTA TCGACGGGCC GTCCAAGGTG AAGATGGACT GTCAGGAATG CCCAGAGGGC 
TACAAAGTCA CCTACACACC TATGGCCCCT GGAAACTACC TAATTTCTAT TAAATACGGT 
GGACCGTACC ATATTGTTGG TAGTCCATTC AAAGCTAAAA TTACTGGCTC CCGATTGGTC 
AACAGCCACA GCATGCATGA GACTTCATCA GTACTGGTTG ACCCTGTGAC CCGCAGCATG 
ACGACCAGCC AGCAGGCAGC GCCAGGCTGG GCCAGCTCTG ATGCCAGCCG CGTAGTGGCC 
AAAGGTTTGG GGCTCAACAA GGGCTTCATA GGACAGAAGA ACACTTTCAG TGTGGACTGT 
AGTAAAGCAG GGAGGAACAT GCTCCTGGTC GGAGTGGATG GACCCAAAGT GCCCTGTGAG 
GAGATCCTGG TGAAACACCT GGGCAACCGC CTCTACAACG TTTCCTACCA GCTGAAGGAG 
AAAGGAGAGT ACATTCTGGT GGTCAAATGG GGTGATGAGC ACATCCCGGG CAGCCCGTAT 
CACATCACTG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

flna ( XM_009296771.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295046.1 Danio rerio filamin A, alpha (actin binding protein 280) (flna), transcript variant X2, mRNA.

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CloneID	ODa43842
Clone ID Related Accession (Same CDS sequence)	XM_021474524.1 , XM_009296771.2 , XM_009296771.3
Accession Version	XM_009296771.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7635bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	filamin-A isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007134.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009296771.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTCAAC ACACACGCCC CCACCAGTCG AGCGCCGCCG CTCCCCTATC CAACGCTTCT 
CTCTCCCCGG ACAAGGACGC CGACATGCCC GCTACAGAGA AAGATCTCGC CGAGGACGCG 
CCATGGAAGA AAATCCAGCA AAATACATTC ACGCGTTGGT GCAACGAGCA TCTGAAATGT 
GTCAACAAGC GCATAGCGAA CCTGCAGACG GACCTGAGCG ACGGGCTCCG GCTGATCGCG 
CTTCTGGAGG TGCTCAGCCA GAAGAAGATG TTCCGCAAGT ACAACCAGCG CCCTACCTTC 
AGACAGATGC AGCTGGAGAA CGTCTCCGTG GCGCTGGAGT TCCTGGACAT TGAAAACATC 
AAGCTGGTCT CCATTGACAG TAAGGCCATA GTGGATGGTA ACCTGAAGCT GATCCTGGGT 
CTGATATGGA CTCTTATTCT GCATTATTCC ATCTCCATGC CCATGTGGGA CGAGGAAGAG 
GAGAGCGAGG AAAGCAAGCA GAAGACGCCC AAGCAGAGGC TCCTCGGATG GATCCAGAAC 
AAGCTGCCCG AGCTGCCAAT CACCAACTTC AGCCGCGATT GGCAGTCAGG GAAAGCCCTT 
GGCGCTCTGG TGGACAGTTG TGCTCCAGGT CTGTGTCCAG ACTGGGATTC CTGGGACCAG 
ACCAAGCCTG TGGACAATGC CAGAGAGGCC ATGCAGCAGG CTGATGACTG GCTCGGTGTT 
CCTCAGGTCA TCACACCAGA AGAAATCGTT GATCCCAATG TGGACGAGCA CTCCGTTATG 
ACTTACCTGT CCCAGTTTCC CAAAGCCAAA CTCAAGCCTG GTGCCCCTCT GAGGCCCAAA 
CTCAATCCCA AAAAGGCCCG TGCTTATGGA CCAGGTATTG AGCCAACAGG TAATGTTGTG 
ATGAAGAAGG CTGTGTTCAC TGTTGAGACC ATCAGCGCTG GTCAGGGTGA GGTACTGGTT 
TATGTCGAGG ACCCCGCTGG ACACCGCGAG GAGGCCAAAG TCACAGCCAA CAATGACAAG 
AACCGCACCT ACTCAGTATT CTATGTGCCA AAAGTCACTG GACAACACAA GGTGACAGTG 
CTGTTTGCAG GGCAACACAT CTCTAAGAGC CCGTTTGAGG TGGATGTGGG AATGGCTCAG 
GGAGACTCCA GCAAGGTCAC TGCCCAGGGC CCAGGACTCG AGCCTGCAGG CAACATTGCC 
AACAAAACCA CATACTTTGA TGTCTACACA GCTGGTGCTG GAGTAGGAGA GGTGGAGGTG 
GTCATCATGG ACCCCAGTGG AAAGAAGGAC ACGGTTGAGT GCAGTGTCGA GGATAAAGGC 
AACAGCAGCT ACCGATGCAC CTACAAGCCC ACTCAGGAGG GACAGCACAT CATTTACATC 
ACCTTTGCCG GGGGACAGAT CTCCAAGAGC CCTTACACTG TCAATGTGGG AGAGGCCTGT 
AATCCAAGCC TGTGTACAGC CAAGGGCCGC GGTCTGCAGC CTAAAGGCCT GAGGGTCAAG 
GAGACCGCCG AGTTTAAGGT TTACACCAAA GGAGCAGGAA CCGGAGACCT CAAAGTTACC 
ATCAAAGGGC CCAAGGGTCT CGAGGAGCCC TGTAAGAAGA AGGATTTGGG AGACGGCGTG 
TACAGCTTTG ATTATTACCC CTCCACACCT GGAAATTACA TCATCACCAT CACATGGGGC 
GGTCAACACA TCCCACGCAG CCCGTTTGAG GTGAAGATCG GGTCTGAAGC AGGACCGCAG 
CAGGTCAGGG CGTGGGGTCC AGGACTAGAG AGTGGCGTTG TGGGCAAATC TGCTGACTTT 
GTTGTGGAAG CTGTTGGTGA TGATGTGGGC ACTTTAGGTT TCTCTGTTGA AGGCCCTTCC 
CCGGCTAAGA TTGAATGTGA TGATAAGGGA GATGGTTCAT GTGACGTGAG GTACTGGCCC 
ACCGAACCGG GTGAATATGC AGTTCATGTG CTCTGTAAGA ATGAGGACAT CCAGCTCAGC 
CCTTTCATGG CTGAAATCAT GCCCGCCCCA GGAAAAGACT TTTATCCTGA CAAGGTGAAG 
GCTTATGGCC CCGGTCTGCA GAGCTCAGGC CTGTCAGTTG GCAAACCAGC CGAGTTTACC 
GTGGATGCCA AACTGGGTGG CAAAGCTCCT CTGAAGATTC AAGCTCAGGA CCGTGATGGA 
AACCCAGTCG ATGTGCAGGT GAAGGACAAT GGAAATGGGA CATACAGCTG CAGCTACACC 
CCTCGCAAAC CAGTCAAACA CACTGTGATG GTGTCCTGGG GCGGCGTCAA CATCCCAGAG 
AGCCCGTTCA GAATGAACAT TGGGGCAGGG TGTCATCCCA ATAAGGTGAA AGTATCTGGA 
CCTGGAGTGG CCAAGACAGG CCTCAAAGCA TATGATCCAA CATACTTTAC TGTGGATTGT 
GCTGAGGCTG GACAGGGCGA CATCAGCATA GGCATCAAAT GTGCTGCAGG TGTGGTGGGA 
CCTGCTGAGG CTGACATAGA CTTCGACATC ATTAGAAATG ACAACGACAC ATTTACTGTT 
AAATACACTC CTCCTGGAGC GGGCAGCTAC ACCATCATGG TGCTGTTTGC TGATCAGACC 
ATTCCCATGA CACCCATCAG AATTAAGGTT GATCCCTCTC ATGATGCAAG CAAGGTTAAA 
GCTGAGGGGC CTGGACTTAG CCGTTCTGGT GTGGAGCTGA ACAAACCCAC TCACTTCACT 
GTGAACACAA AGGCTGCAGG CAAGGCCAAG CTTGATGCTC AGTTCACTGG ACCCAACAAG 
GGAGATGCAG TCCGTGACTT CGACATAATC GACAACCATG ATGGCACTCA CACTGTCAAA 
TATACCCCTG TCCAACAGGG CAACATGGGT TTGAATGTCA CCTACGGTGG TGACCCTATT 
CCCAAGAGCC CATTTGCAGT AGCAATTGCC CCCTCTCTGG ACCTCAGTAA AGTTAAAGTG 
GCTGGTCTTG GAAGTAAAAT GACTGTTGGA AAGGATCAGG AGATCACAGT CAAATCTAAA 
GGTGCAGGCG GTCAGGGCAA AGTTGGCGCA AAGGTTACTG GTCCATCTGC TAAATCAGTG 
CCCTGTAAAG TTGAACCAGG CCTGAGCCCA GAAACCAGCC AAGTTCGTTT TATCCCAAGA 
GATAAGGGAC CCTATGAGGT GGAACTGACA TATGATGGCG CCCCCATCCC TGGCAGTCCA 
TTCCCCATCG AAGCCATTGC ACCTACTGAC CCATCCAAGG TGCGATGCTC TGGGCCAGGA 
CTGGAGCGGG CGAAGGTTGG CGAGCCTGGA CAGTTTGTGG TGGATTGCAC TAATGCTGGC 
CCTGCTGAGT TGACCATCGA GATCATCTCT GACAATGGCA CTGAGGCTGA AGTCCACATT 
CAAGACAACG GCGACGGGAC CTACACCATC ACTTACGTCC CTCTCTACCC TGGAGCCTAT 
ACCCTCACCA TCCGCTACGG GGACCAGGAT GTGCCAAACT TCCCAGCAAG ACTCCATGTG 
GAGCCTGCTG TGGAGACCAG TGGAGTGAAA GTGTTCGGAC CCGGAGTGGA GGGCAAAGGT 
GTTTTCAGGG AAGCCACTAC AGATTTCACT GTGGATGCCC GTGCGCTCAC TAAAACCGGA 
GGCAATCACA TCAAAACCTG CATCAACAAC CCATCGGGCA ACCGCACTGA AGCACTGATC 
CGAGATCTGG GAGACGGCAC ATATCAAGTG GAGTACACGC CTTATGAGGA GGGTACACAC 
AGTGTTGAGG TTGCATATGA CAGCTCCCCG GTTCCCAAAA GCCCCTTCCG TGTGCCAGTG 
ACCGAGGGCT GTGACCCGGC GCGTGTGCGT GTGCATGGTC CAGGACTTCA GTCTGGCATC 
ACCAACAAAC CTAACAAATT TACCGTGGAG ACCCGTGGGG CTGGTACTGG TGGACTGGGG 
CTGGCTATGG AAGGACCTTC TGAGGCCAAA ATGTCCTGCA CCGATAACAA AGATGGCAGC 
TGTTGTGTTG AATACATCCC ATATGAGCCA GGGACATACA ACCTCAATGT CACCTATGGA 
GGCCAACCAA TCACTGGAAG CCCTTTCTCT GTACCTGTCC ATGATACTGT TGACCCAACT 
AAAGTGAAAT GTCAGGGCCA GGGGCTTGGA AACAACGTGC GTGCCAATAT CCCACAGGTC 
TTTTCTGTGG ATGCAAGCAA GGCTGGTATG GCGCCACTGC AGGTCAGAGT TCAGGGACCC 
AAAGGTGTTG TGGAGCCTGC TGAGGTGGTG GATAATGGTG ACCAGACTCA CACAGTCAGC 
TATGTGCCCA CAAGAGAGGG GCCATACTCC ATTAATGTTC TGTATGCTGA TGAGGAGATC 
CCACAGAGTC CTTATAAGGT GAAGGTTCTG CCCACTCATG ACGCAAGTAA AGTCCGTTGC 
AGCGGTCCTG GCCTGAATAC CACTGGTGTT CCTGCCAGTC TGCCCGTGGA GTTCACTATT 
GACGCCAAAG ATGCAGGAGA GGGACTGTTG GCCGTCCAGA TAACTGACCC TGATGGGAAG 
CCAAAGAAGG CTAACATTCG TGATAATCAG GACGGGACCT ACCTTGTGTC CTATGTGCCT 
GACATGACCG GCCGTTACAC CATTCTTATT AAGTATGGTG GTGATGAGAT CCCGTATTCT 
CCATATCGCA TTCGAGCCCT GCCTACTGGA GACGCCAGCA AGTGTACAGT CACAGGAGCT 
GGAGTTGGTC CGACTATTCA GATTGGAGAG CAGACTGTCA TCACTGTGGA TGCGAAGGCA 
GCTGGGAAGG GCAAGGTGAC CTGTACCGTG TGCACACCTG ATGGAGGCGA GGTGGATGTG 
GACGTCGTGG AAAATGAGGA TGGAACGTTT GACATCTTTT ACACTGCACC GCAGCCTGGG 
AAATACGTGA TCTGTGTGCG CTTCGGTGGA GAGCACATCC CTAACAGCCC CTTCCAAGTC 
ACGGCTTTGG AAGGTGACTC TCCTGACCAA CTAATGCAGC AGAGCCAGAA CCTGCAGTAC 
GCCTACGCGC CCAACATGGG CCAGACATGG GCTACAGATG GGCAATTGGG TATGAATGGA 
CTGGATGTAG CTGGACTGAG ACCCTTTGAC TTGGTCATTC CTTTCACCAT TCAGAAAGGA 
GAAATTACAG GTGATGTACG AATGCCCTCT GGAAAAGTGG CCAAGCCTGA TATTACAGAC 
AACAAGGATG GCACAGTCAC AGTGAAATAT GCCCCAACTG AGGCGGGCCT GCATGAGATG 
GACATTAAAT ATGATGGAAT ACACATTCCA GGAAGTCCAT TGCAATTCTA CGTGGATTAT 
GTCAACAGCG GTCATGTGAC TGCATATGGT CCTGGTCTAA TTCATGGCAT GGTCAATAAG 
CCTGCAGTCT TCACAGTCAA CACAAAGGAT GCTGGAGAGG GTGGTCTGTC TCTGGCCATC 
GAGGGGCCAT CAAAGGCAGA TATCAGCTGC ACTGATAATC AGGACGGTAC CTGCACTGTT 
TCATACCTCC CTGTTCTTCC CGGAGACTAC AATATCATTG TTAAATACAA TGACAAGAAC 
ATCCCCGGCA GCCCTTTCAT GTCCAAAATC ACAGGTGACG ACTCGATGCG CATGTCTCAC 
CTGAAGGTGG GCTCAGCTGC TGACATCCCT CTGGACATTG GTGAGCTTGA CCTCAGTCAG 
CTGACAGCCA CACTCACCAC CCCATCCGGC CGAGAGGAGC CTTGCCTGCT CAAGATGCTC 
AGAAATGGGC ATGTGGGAAT TTCCTTTGTG CCCAAGGAAA TTGGCGAGCA TCTTGTTAAC 
ATTAAGAAAA ATGGACGCCA TATTCCTAGT AGCCCCATTT CTGTCATGAT CAACCAATCA 
GAGATCGGTG ATGCCAGCCG TGTACGAGTA ACTGGTCAAG GCCTGAGTGA AGCTCGCACC 
TTTGAGCCCG CAGAATTCAT CATTGACACT AGAGATGCAG GATATGGTGG ACTCAGTTTG 
TCTATTGAGG GACCTAGTAA AGTGGATATC AACACTGAAG ATCAGGAAGA TGGCACCTGC 
AAAGTGACAT ATTGCCCCAC AGAGCCTGGA AACTACATAA TTAACATCAA ATTTGCAGAC 
CAGCATGTGC CAGGAAGTGC TTTCACTGTG AAAGTGACAG GCGAGGGTCG GATGAAGGAG 
AGCATCACCC GCAAAAGAAG AGCTGCTTCT GTGGCCAATG TTGGCAGCCA GTGCGACCTC 
AGTCTAAAGA TCCCTGAGAT CAGCATTGCA GACATGACCG CTCAGGTGAC CAGCCCATCT 
GGCAAAGTCC ACAAGGCAGA GATAATGGAG GGAGAAAACA ACACCTACTG CATCCGATTC 
GTGCCCACAG AGATGGGTGT GCACACAGTA AGCGTGAAGT ATCAAGGTCA GCATGTGCCT 
GGATCCCCCT TCCAGTTCAC GGTGGGACCT CTTGGAGAAG GAGGAGCTCA TAAAGTCAGA 
GCTGGAGGCC CAGGATTAGA GAGGGCAGAA GCTGGTGTAC CAGCGGAGTT CAGCATTTGG 
ACTCGTGAAG CAGGAGCTGG TGGTCTGTCC ATCGCAGTGG AAGGACCCAG CAAAGCAGAG 
ATTGCTTTTG AAGACCGTAA AGATGGATCC AGTGGTGTCT CTTACATCGT CCAGGAGCCT 
GGCGATTATG AAGTATCCAT CAAGTTCAAT GACGAGCACA TCCCTGACAG TCCGTTTGTG 
GTGCCTGTGG CCTCGCCATC TGACGATGCC CGCCGTCTCA CTGTTGCCAG TCTTCAGGAG 
TCCGGGTTAA AGGTGAACCA GCCGGCCTCT TTTGCTGTGA GCCTAAATGG GGCGAAAGGA 
GTCATCGATG CCAAAGTTCA CAGTCCATCA GGAGCACTTG AAGAGTGCTG CGTCACCGAG 
ATCGATGAAG ATAAATATGC TGTGAGATTC ATTCCCAGGG AGAATGGATT GTACCTGATA 
GATGTGAAAT TTAATGGTTC TCACATCCCT GGAAGCCCCT TCAAGATCCG TGTTGGTGAG 
ACGGGTCAGG CTGGAGATCC AGGAATGGTG TCTGCTTATG GAGCTGGTCT GGAAGGAGGC 
ACTACTGGAT CGCCTTGTGA GTTCATTGTA AACACCAGTT CAGCTGGCCC TGGAGCTTTG 
GCGGTGACTA TCGACGGGCC GTCCAAGGTG AAGATGGACT GTCAGGAATG CCCAGAGGGC 
TACAAAGTCA CCTACACACC TATGGCCCCT GGAAACTACC TAATTTCTAT TAAATACGGT 
GGACCGTACC ATATTGTTGG TAGTCCATTC AAAGCTAAAA TTACTGGCTC CCGATTGGTC 
AACAGCCACA GCATGCATGA GACTTCATCA GTACTGGTTG ACCCTGTGAC CCGCAGCATG 
ACGACCAGCC AGCAGGCAGC GCCAGGCTGG GCCAGCTCTG ATGCCAGCCG CGTAGTGGCC 
AAAGGTTTGG GGCTCAACAA GGGCTTCATA GGACAGAAGA ACACTTTCAG TGTGGACTGT 
AGTAAAGCAG GGAGGAACAT GCTCCTGGTC GGAGTGGATG GACCCAAAGT GCCCTGTGAG 
GAGATCCTGG TGAAACACCT GGGCAACCGC CTCTACAACG TTTCCTACCA GCTGAAGGAG 
AAAGGAGAGT ACATTCTGGT GGTCAAATGG GGTGATGAGC ACATCCCGGG CAGCCCGTAT 
CACATCACTG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295046.1
CDS	356..7990
Translation	MSQHTRPHQSSAAAPLSNASLSPDKDADMPATEKDLAEDAPWKKIQQNTFTRWCNEHLKCVNKRIANLQTDLSDGLRLIALLEVLSQKKMFRKYNQRPTFRQMQLENVSVALEFLDIENIKLVSIDSKAIVDGNLKLILGLIWTLILHYSISMPMWDEEEESEESKQKTPKQRLLGWIQNKLPELPITNFSRDWQSGKALGALVDSCAPGLCPDWDSWDQTKPVDNAREAMQQADDWLGVPQVITPEEIVDPNVDEHSVMTYLSQFPKAKLKPGAPLRPKLNPKKARAYGPGIEPTGNVVMKKAVFTVETISAGQGEVLVYVEDPAGHREEAKVTANNDKNRTYSVFYVPKVTGQHKVTVLFAGQHISKSPFEVDVGMAQGDSSKVTAQGPGLEPAGNIANKTTYFDVYTAGAGVGEVEVVIMDPSGKKDTVECSVEDKGNSSYRCTYKPTQEGQHIIYITFAGGQISKSPYTVNVGEACNPSLCTAKGRGLQPKGLRVKETAEFKVYTKGAGTGDLKVTIKGPKGLEEPCKKKDLGDGVYSFDYYPSTPGNYIITITWGGQHIPRSPFEVKIGSEAGPQQVRAWGPGLESGVVGKSADFVVEAVGDDVGTLGFSVEGPSPAKIECDDKGDGSCDVRYWPTEPGEYAVHVLCKNEDIQLSPFMAEIMPAPGKDFYPDKVKAYGPGLQSSGLSVGKPAEFTVDAKLGGKAPLKIQAQDRDGNPVDVQVKDNGNGTYSCSYTPRKPVKHTVMVSWGGVNIPESPFRMNIGAGCHPNKVKVSGPGVAKTGLKAYDPTYFTVDCAEAGQGDISIGIKCAAGVVGPAEADIDFDIIRNDNDTFTVKYTPPGAGSYTIMVLFADQTIPMTPIRIKVDPSHDASKVKAEGPGLSRSGVELNKPTHFTVNTKAAGKAKLDAQFTGPNKGDAVRDFDIIDNHDGTHTVKYTPVQQGNMGLNVTYGGDPIPKSPFAVAIAPSLDLSKVKVAGLGSKMTVGKDQEITVKSKGAGGQGKVGAKVTGPSAKSVPCKVEPGLSPETSQVRFIPRDKGPYEVELTYDGAPIPGSPFPIEAIAPTDPSKVRCSGPGLERAKVGEPGQFVVDCTNAGPAELTIEIISDNGTEAEVHIQDNGDGTYTITYVPLYPGAYTLTIRYGDQDVPNFPARLHVEPAVETSGVKVFGPGVEGKGVFREATTDFTVDARALTKTGGNHIKTCINNPSGNRTEALIRDLGDGTYQVEYTPYEEGTHSVEVAYDSSPVPKSPFRVPVTEGCDPARVRVHGPGLQSGITNKPNKFTVETRGAGTGGLGLAMEGPSEAKMSCTDNKDGSCCVEYIPYEPGTYNLNVTYGGQPITGSPFSVPVHDTVDPTKVKCQGQGLGNNVRANIPQVFSVDASKAGMAPLQVRVQGPKGVVEPAEVVDNGDQTHTVSYVPTREGPYSINVLYADEEIPQSPYKVKVLPTHDASKVRCSGPGLNTTGVPASLPVEFTIDAKDAGEGLLAVQITDPDGKPKKANIRDNQDGTYLVSYVPDMTGRYTILIKYGGDEIPYSPYRIRALPTGDASKCTVTGAGVGPTIQIGEQTVITVDAKAAGKGKVTCTVCTPDGGEVDVDVVENEDGTFDIFYTAPQPGKYVICVRFGGEHIPNSPFQVTALEGDSPDQLMQQSQNLQYAYAPNMGQTWATDGQLGMNGLDVAGLRPFDLVIPFTIQKGEITGDVRMPSGKVAKPDITDNKDGTVTVKYAPTEAGLHEMDIKYDGIHIPGSPLQFYVDYVNSGHVTAYGPGLIHGMVNKPAVFTVNTKDAGEGGLSLAIEGPSKADISCTDNQDGTCTVSYLPVLPGDYNIIVKYNDKNIPGSPFMSKITGDDSMRMSHLKVGSAADIPLDIGELDLSQLTATLTTPSGREEPCLLKMLRNGHVGISFVPKEIGEHLVNIKKNGRHIPSSPISVMINQSEIGDASRVRVTGQGLSEARTFEPAEFIIDTRDAGYGGLSLSIEGPSKVDINTEDQEDGTCKVTYCPTEPGNYIINIKFADQHVPGSAFTVKVTGEGRMKESITRKRRAASVANVGSQCDLSLKIPEISIADMTAQVTSPSGKVHKAEIMEGENNTYCIRFVPTEMGVHTVSVKYQGQHVPGSPFQFTVGPLGEGGAHKVRAGGPGLERAEAGVPAEFSIWTREAGAGGLSIAVEGPSKAEIAFEDRKDGSSGVSYIVQEPGDYEVSIKFNDEHIPDSPFVVPVASPSDDARRLTVASLQESGLKVNQPASFAVSLNGAKGVIDAKVHSPSGALEECCVTEIDEDKYAVRFIPRENGLYLIDVKFNGSHIPGSPFKIRVGETGQAGDPGMVSAYGAGLEGGTTGSPCEFIVNTSSAGPGALAVTIDGPSKVKMDCQECPEGYKVTYTPMAPGNYLISIKYGGPYHIVGSPFKAKITGSRLVNSHSMHETSSVLVDPVTRSMTTSQQAAPGWASSDASRVVAKGLGLNKGFIGQKNTFSVDCSKAGRNMLLVGVDGPKVPCEEILVKHLGNRLYNVSYQLKEKGEYILVVKWGDEHIPGSPYHITV

Target ORF information:

RefSeq Version	XM_009296771.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio filamin A, alpha (actin binding protein 280) (flna), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009296771.3

ORF Insert Sequence:

ATGAGTCAAC ACACACGCCC CCACCAGTCG AGCGCCGCCG CTCCCCTATC CAACGCTTCT 
CTCTCCCCGG ACAAGGACGC CGACATGCCC GCTACAGAGA AAGATCTCGC CGAGGACGCG 
CCATGGAAGA AAATCCAGCA AAATACATTC ACGCGTTGGT GCAACGAGCA TCTGAAATGT 
GTCAACAAGC GCATAGCGAA CCTGCAGACG GACCTGAGCG ACGGGCTCCG GCTGATCGCG 
CTTCTGGAGG TGCTCAGCCA GAAGAAGATG TTCCGCAAGT ACAACCAGCG CCCTACCTTC 
AGACAGATGC AGCTGGAGAA CGTCTCCGTG GCGCTGGAGT TCCTGGACAT TGAAAACATC 
AAGCTGGTCT CCATTGACAG TAAGGCCATA GTGGATGGTA ACCTGAAGCT GATCCTGGGT 
CTGATATGGA CTCTTATTCT GCATTATTCC ATCTCCATGC CCATGTGGGA CGAGGAAGAG 
GAGAGCGAGG AAAGCAAGCA GAAGACGCCC AAGCAGAGGC TCCTCGGATG GATCCAGAAC 
AAGCTGCCCG AGCTGCCAAT CACCAACTTC AGCCGCGATT GGCAGTCAGG GAAAGCCCTT 
GGCGCTCTGG TGGACAGTTG TGCTCCAGGT CTGTGTCCAG ACTGGGATTC CTGGGACCAG 
ACCAAGCCTG TGGACAATGC CAGAGAGGCC ATGCAGCAGG CTGATGACTG GCTCGGTGTT 
CCTCAGGTCA TCACACCAGA AGAAATCGTT GATCCCAATG TGGACGAGCA CTCCGTTATG 
ACTTACCTGT CCCAGTTTCC CAAAGCCAAA CTCAAGCCTG GTGCCCCTCT GAGGCCCAAA 
CTCAATCCCA AAAAGGCCCG TGCTTATGGA CCAGGTATTG AGCCAACAGG TAATGTTGTG 
ATGAAGAAGG CTGTGTTCAC TGTTGAGACC ATCAGCGCTG GTCAGGGTGA GGTACTGGTT 
TATGTCGAGG ACCCCGCTGG ACACCGCGAG GAGGCCAAAG TCACAGCCAA CAATGACAAG 
AACCGCACCT ACTCAGTATT CTATGTGCCA AAAGTCACTG GACAACACAA GGTGACAGTG 
CTGTTTGCAG GGCAACACAT CTCTAAGAGC CCGTTTGAGG TGGATGTGGG AATGGCTCAG 
GGAGACTCCA GCAAGGTCAC TGCCCAGGGC CCAGGACTCG AGCCTGCAGG CAACATTGCC 
AACAAAACCA CATACTTTGA TGTCTACACA GCTGGTGCTG GAGTAGGAGA GGTGGAGGTG 
GTCATCATGG ACCCCAGTGG AAAGAAGGAC ACGGTTGAGT GCAGTGTCGA GGATAAAGGC 
AACAGCAGCT ACCGATGCAC CTACAAGCCC ACTCAGGAGG GACAGCACAT CATTTACATC 
ACCTTTGCCG GGGGACAGAT CTCCAAGAGC CCTTACACTG TCAATGTGGG AGAGGCCTGT 
AATCCAAGCC TGTGTACAGC CAAGGGCCGC GGTCTGCAGC CTAAAGGCCT GAGGGTCAAG 
GAGACCGCCG AGTTTAAGGT TTACACCAAA GGAGCAGGAA CCGGAGACCT CAAAGTTACC 
ATCAAAGGGC CCAAGGGTCT CGAGGAGCCC TGTAAGAAGA AGGATTTGGG AGACGGCGTG 
TACAGCTTTG ATTATTACCC CTCCACACCT GGAAATTACA TCATCACCAT CACATGGGGC 
GGTCAACACA TCCCACGCAG CCCGTTTGAG GTGAAGATCG GGTCTGAAGC AGGACCGCAG 
CAGGTCAGGG CGTGGGGTCC AGGACTAGAG AGTGGCGTTG TGGGCAAATC TGCTGACTTT 
GTTGTGGAAG CTGTTGGTGA TGATGTGGGC ACTTTAGGTT TCTCTGTTGA AGGCCCTTCC 
CCGGCTAAGA TTGAATGTGA TGATAAGGGA GATGGTTCAT GTGACGTGAG GTACTGGCCC 
ACCGAACCGG GTGAATATGC AGTTCATGTG CTCTGTAAGA ATGAGGACAT CCAGCTCAGC 
CCTTTCATGG CTGAAATCAT GCCCGCCCCA GGAAAAGACT TTTATCCTGA CAAGGTGAAG 
GCTTATGGCC CCGGTCTGCA GAGCTCAGGC CTGTCAGTTG GCAAACCAGC CGAGTTTACC 
GTGGATGCCA AACTGGGTGG CAAAGCTCCT CTGAAGATTC AAGCTCAGGA CCGTGATGGA 
AACCCAGTCG ATGTGCAGGT GAAGGACAAT GGAAATGGGA CATACAGCTG CAGCTACACC 
CCTCGCAAAC CAGTCAAACA CACTGTGATG GTGTCCTGGG GCGGCGTCAA CATCCCAGAG 
AGCCCGTTCA GAATGAACAT TGGGGCAGGG TGTCATCCCA ATAAGGTGAA AGTATCTGGA 
CCTGGAGTGG CCAAGACAGG CCTCAAAGCA TATGATCCAA CATACTTTAC TGTGGATTGT 
GCTGAGGCTG GACAGGGCGA CATCAGCATA GGCATCAAAT GTGCTGCAGG TGTGGTGGGA 
CCTGCTGAGG CTGACATAGA CTTCGACATC ATTAGAAATG ACAACGACAC ATTTACTGTT 
AAATACACTC CTCCTGGAGC GGGCAGCTAC ACCATCATGG TGCTGTTTGC TGATCAGACC 
ATTCCCATGA CACCCATCAG AATTAAGGTT GATCCCTCTC ATGATGCAAG CAAGGTTAAA 
GCTGAGGGGC CTGGACTTAG CCGTTCTGGT GTGGAGCTGA ACAAACCCAC TCACTTCACT 
GTGAACACAA AGGCTGCAGG CAAGGCCAAG CTTGATGCTC AGTTCACTGG ACCCAACAAG 
GGAGATGCAG TCCGTGACTT CGACATAATC GACAACCATG ATGGCACTCA CACTGTCAAA 
TATACCCCTG TCCAACAGGG CAACATGGGT TTGAATGTCA CCTACGGTGG TGACCCTATT 
CCCAAGAGCC CATTTGCAGT AGCAATTGCC CCCTCTCTGG ACCTCAGTAA AGTTAAAGTG 
GCTGGTCTTG GAAGTAAAAT GACTGTTGGA AAGGATCAGG AGATCACAGT CAAATCTAAA 
GGTGCAGGCG GTCAGGGCAA AGTTGGCGCA AAGGTTACTG GTCCATCTGC TAAATCAGTG 
CCCTGTAAAG TTGAACCAGG CCTGAGCCCA GAAACCAGCC AAGTTCGTTT TATCCCAAGA 
GATAAGGGAC CCTATGAGGT GGAACTGACA TATGATGGCG CCCCCATCCC TGGCAGTCCA 
TTCCCCATCG AAGCCATTGC ACCTACTGAC CCATCCAAGG TGCGATGCTC TGGGCCAGGA 
CTGGAGCGGG CGAAGGTTGG CGAGCCTGGA CAGTTTGTGG TGGATTGCAC TAATGCTGGC 
CCTGCTGAGT TGACCATCGA GATCATCTCT GACAATGGCA CTGAGGCTGA AGTCCACATT 
CAAGACAACG GCGACGGGAC CTACACCATC ACTTACGTCC CTCTCTACCC TGGAGCCTAT 
ACCCTCACCA TCCGCTACGG GGACCAGGAT GTGCCAAACT TCCCAGCAAG ACTCCATGTG 
GAGCCTGCTG TGGAGACCAG TGGAGTGAAA GTGTTCGGAC CCGGAGTGGA GGGCAAAGGT 
GTTTTCAGGG AAGCCACTAC AGATTTCACT GTGGATGCCC GTGCGCTCAC TAAAACCGGA 
GGCAATCACA TCAAAACCTG CATCAACAAC CCATCGGGCA ACCGCACTGA AGCACTGATC 
CGAGATCTGG GAGACGGCAC ATATCAAGTG GAGTACACGC CTTATGAGGA GGGTACACAC 
AGTGTTGAGG TTGCATATGA CAGCTCCCCG GTTCCCAAAA GCCCCTTCCG TGTGCCAGTG 
ACCGAGGGCT GTGACCCGGC GCGTGTGCGT GTGCATGGTC CAGGACTTCA GTCTGGCATC 
ACCAACAAAC CTAACAAATT TACCGTGGAG ACCCGTGGGG CTGGTACTGG TGGACTGGGG 
CTGGCTATGG AAGGACCTTC TGAGGCCAAA ATGTCCTGCA CCGATAACAA AGATGGCAGC 
TGTTGTGTTG AATACATCCC ATATGAGCCA GGGACATACA ACCTCAATGT CACCTATGGA 
GGCCAACCAA TCACTGGAAG CCCTTTCTCT GTACCTGTCC ATGATACTGT TGACCCAACT 
AAAGTGAAAT GTCAGGGCCA GGGGCTTGGA AACAACGTGC GTGCCAATAT CCCACAGGTC 
TTTTCTGTGG ATGCAAGCAA GGCTGGTATG GCGCCACTGC AGGTCAGAGT TCAGGGACCC 
AAAGGTGTTG TGGAGCCTGC TGAGGTGGTG GATAATGGTG ACCAGACTCA CACAGTCAGC 
TATGTGCCCA CAAGAGAGGG GCCATACTCC ATTAATGTTC TGTATGCTGA TGAGGAGATC 
CCACAGAGTC CTTATAAGGT GAAGGTTCTG CCCACTCATG ACGCAAGTAA AGTCCGTTGC 
AGCGGTCCTG GCCTGAATAC CACTGGTGTT CCTGCCAGTC TGCCCGTGGA GTTCACTATT 
GACGCCAAAG ATGCAGGAGA GGGACTGTTG GCCGTCCAGA TAACTGACCC TGATGGGAAG 
CCAAAGAAGG CTAACATTCG TGATAATCAG GACGGGACCT ACCTTGTGTC CTATGTGCCT 
GACATGACCG GCCGTTACAC CATTCTTATT AAGTATGGTG GTGATGAGAT CCCGTATTCT 
CCATATCGCA TTCGAGCCCT GCCTACTGGA GACGCCAGCA AGTGTACAGT CACAGGAGCT 
GGAGTTGGTC CGACTATTCA GATTGGAGAG CAGACTGTCA TCACTGTGGA TGCGAAGGCA 
GCTGGGAAGG GCAAGGTGAC CTGTACCGTG TGCACACCTG ATGGAGGCGA GGTGGATGTG 
GACGTCGTGG AAAATGAGGA TGGAACGTTT GACATCTTTT ACACTGCACC GCAGCCTGGG 
AAATACGTGA TCTGTGTGCG CTTCGGTGGA GAGCACATCC CTAACAGCCC CTTCCAAGTC 
ACGGCTTTGG AAGGTGACTC TCCTGACCAA CTAATGCAGC AGAGCCAGAA CCTGCAGTAC 
GCCTACGCGC CCAACATGGG CCAGACATGG GCTACAGATG GGCAATTGGG TATGAATGGA 
CTGGATGTAG CTGGACTGAG ACCCTTTGAC TTGGTCATTC CTTTCACCAT TCAGAAAGGA 
GAAATTACAG GTGATGTACG AATGCCCTCT GGAAAAGTGG CCAAGCCTGA TATTACAGAC 
AACAAGGATG GCACAGTCAC AGTGAAATAT GCCCCAACTG AGGCGGGCCT GCATGAGATG 
GACATTAAAT ATGATGGAAT ACACATTCCA GGAAGTCCAT TGCAATTCTA CGTGGATTAT 
GTCAACAGCG GTCATGTGAC TGCATATGGT CCTGGTCTAA TTCATGGCAT GGTCAATAAG 
CCTGCAGTCT TCACAGTCAA CACAAAGGAT GCTGGAGAGG GTGGTCTGTC TCTGGCCATC 
GAGGGGCCAT CAAAGGCAGA TATCAGCTGC ACTGATAATC AGGACGGTAC CTGCACTGTT 
TCATACCTCC CTGTTCTTCC CGGAGACTAC AATATCATTG TTAAATACAA TGACAAGAAC 
ATCCCCGGCA GCCCTTTCAT GTCCAAAATC ACAGGTGACG ACTCGATGCG CATGTCTCAC 
CTGAAGGTGG GCTCAGCTGC TGACATCCCT CTGGACATTG GTGAGCTTGA CCTCAGTCAG 
CTGACAGCCA CACTCACCAC CCCATCCGGC CGAGAGGAGC CTTGCCTGCT CAAGATGCTC 
AGAAATGGGC ATGTGGGAAT TTCCTTTGTG CCCAAGGAAA TTGGCGAGCA TCTTGTTAAC 
ATTAAGAAAA ATGGACGCCA TATTCCTAGT AGCCCCATTT CTGTCATGAT CAACCAATCA 
GAGATCGGTG ATGCCAGCCG TGTACGAGTA ACTGGTCAAG GCCTGAGTGA AGCTCGCACC 
TTTGAGCCCG CAGAATTCAT CATTGACACT AGAGATGCAG GATATGGTGG ACTCAGTTTG 
TCTATTGAGG GACCTAGTAA AGTGGATATC AACACTGAAG ATCAGGAAGA TGGCACCTGC 
AAAGTGACAT ATTGCCCCAC AGAGCCTGGA AACTACATAA TTAACATCAA ATTTGCAGAC 
CAGCATGTGC CAGGAAGTGC TTTCACTGTG AAAGTGACAG GCGAGGGTCG GATGAAGGAG 
AGCATCACCC GCAAAAGAAG AGCTGCTTCT GTGGCCAATG TTGGCAGCCA GTGCGACCTC 
AGTCTAAAGA TCCCTGAGAT CAGCATTGCA GACATGACCG CTCAGGTGAC CAGCCCATCT 
GGCAAAGTCC ACAAGGCAGA GATAATGGAG GGAGAAAACA ACACCTACTG CATCCGATTC 
GTGCCCACAG AGATGGGTGT GCACACAGTA AGCGTGAAGT ATCAAGGTCA GCATGTGCCT 
GGATCCCCCT TCCAGTTCAC GGTGGGACCT CTTGGAGAAG GAGGAGCTCA TAAAGTCAGA 
GCTGGAGGCC CAGGATTAGA GAGGGCAGAA GCTGGTGTAC CAGCGGAGTT CAGCATTTGG 
ACTCGTGAAG CAGGAGCTGG TGGTCTGTCC ATCGCAGTGG AAGGACCCAG CAAAGCAGAG 
ATTGCTTTTG AAGACCGTAA AGATGGATCC AGTGGTGTCT CTTACATCGT CCAGGAGCCT 
GGCGATTATG AAGTATCCAT CAAGTTCAAT GACGAGCACA TCCCTGACAG TCCGTTTGTG 
GTGCCTGTGG CCTCGCCATC TGACGATGCC CGCCGTCTCA CTGTTGCCAG TCTTCAGGAG 
TCCGGGTTAA AGGTGAACCA GCCGGCCTCT TTTGCTGTGA GCCTAAATGG GGCGAAAGGA 
GTCATCGATG CCAAAGTTCA CAGTCCATCA GGAGCACTTG AAGAGTGCTG CGTCACCGAG 
ATCGATGAAG ATAAATATGC TGTGAGATTC ATTCCCAGGG AGAATGGATT GTACCTGATA 
GATGTGAAAT TTAATGGTTC TCACATCCCT GGAAGCCCCT TCAAGATCCG TGTTGGTGAG 
ACGGGTCAGG CTGGAGATCC AGGAATGGTG TCTGCTTATG GAGCTGGTCT GGAAGGAGGC 
ACTACTGGAT CGCCTTGTGA GTTCATTGTA AACACCAGTT CAGCTGGCCC TGGAGCTTTG 
GCGGTGACTA TCGACGGGCC GTCCAAGGTG AAGATGGACT GTCAGGAATG CCCAGAGGGC 
TACAAAGTCA CCTACACACC TATGGCCCCT GGAAACTACC TAATTTCTAT TAAATACGGT 
GGACCGTACC ATATTGTTGG TAGTCCATTC AAAGCTAAAA TTACTGGCTC CCGATTGGTC 
AACAGCCACA GCATGCATGA GACTTCATCA GTACTGGTTG ACCCTGTGAC CCGCAGCATG 
ACGACCAGCC AGCAGGCAGC GCCAGGCTGG GCCAGCTCTG ATGCCAGCCG CGTAGTGGCC 
AAAGGTTTGG GGCTCAACAA GGGCTTCATA GGACAGAAGA ACACTTTCAG TGTGGACTGT 
AGTAAAGCAG GGAGGAACAT GCTCCTGGTC GGAGTGGATG GACCCAAAGT GCCCTGTGAG 
GAGATCCTGG TGAAACACCT GGGCAACCGC CTCTACAACG TTTCCTACCA GCTGAAGGAG 
AAAGGAGAGT ACATTCTGGT GGTCAAATGG GGTGATGAGC ACATCCCGGG CAGCCCGTAT 
CACATCACTG TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	flna
Entrez Gene ID	562529
Full Name	filamin A, alpha (actin binding protein 280)
Synonyms	fb98b06,wu:fb98b06,wu:fd09g03,wu:fd49a06
General protein information	Preferred Names filamin A, alpha (actin binding protein 280) Names filamin-A fd09g03
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

flna cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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