nwd2 cDNA ORF clone, Danio rerio(zebrafish)

The following nwd2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nwd2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa13555 XM_685709.8
Latest version!
Danio rerio NACHT and WD repeat domain containing 2 (nwd2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa13556 XM_005172360.3
Latest version!
Danio rerio NACHT and WD repeat domain containing 2 (nwd2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa43788 XM_009303553.3
Latest version!
Danio rerio NACHT and WD repeat domain containing 2 (nwd2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
View Old Accession Versions >>
ODa43788 XM_009303553.2 Danio rerio NACHT and WD repeat domain containing 2 (nwd2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa13555 XM_685709.7 Danio rerio NACHT and WD repeat domain containing 2 (nwd2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $1021.30
$1459.00
ODa13556 XM_005172360.2 Danio rerio NACHT and WD repeat domain containing 2 (nwd2), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa13555
    Clone ID Related Accession (Same CDS sequence) XM_685709.8 , XM_685709.7
    Accession Version XM_685709.8 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5244bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product NACHT and WD repeat domain-containing protein 2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_685709.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAAGAGGA TGAACCCACG GTGGAAGTCG CCACCCATGT GGCCATCCGG AGCGGGCAGC 
    AGGCAGCCCT GTCCCCGTGA ATCTGCACTG CGCAGAGCAG CCATCAGTGG CAACGTCCTT
    GCCCTCCCAC CATTCCATGT CCCCACGGGC AGGAGTGTCC GGGTCTTCAT CTGTGCCAAC
    CCTGACGATA CAGAAGCAGA GAGAAATGCT CTGAAAGAAC ATGTCTACCC CAAACTACGA
    GACTTCTGCC GAGAGAACTA TGGCATTGAA TTCCAGGTGG TGGACCTGTA CTGGGGCATT
    GATCCAGAGG AGTGGGACAG TCCGGAGCTG CAGAGACTGC GGATGAAACT ACTGGAAGAA
    TGTCTGAAGA CTTCAGCTGG TCCATGCTTT GTTGGGCTCA TAGGAGAGAA GTATGGCAGC
    ATTCGGGTCC CAGGGGAGGT GGAGTCAGCT GAGTTTGAGA TGATTTTGGA TGCGGCAGTG
    GAGGCGGGAC TAGACACACG GATCTTGGAG GAGTGGTACT GTCGAGATGA GAACTCAGTC
    CCACCTGCCT ACTACCTAAA ACCAAAAGCT GAGATGCTGA AAAACTATCA GAATTCTATG
    GAGTCAAACA CCGTAGCAAA ATCAAAGAAC GACAAGGCAT GGAGGGCAGT CTCAGAGGAA
    ATCAAGAGGA TCTTCAGAAC CTCTGTTTTA CAGCTGCAGG AGAAGGGGAC CATGAAGAGT
    GGTCAAGCCA AGAAATTTCT CTGCTCAGCA TTAGAGGATG AACTGGACTT CGCATTGGGC
    AAGCAGACCC CAGCCTTTCT CAAAAAATGT GTTTGTTACA TTCGGAAGAT ATCTAACTTT
    GATCGTTTCG CTAAACTCCC CGAAATGGCA CGTTACATGG ACATTGTGGT GAGCGATGAC
    CGTGTCATGA GAAACCAGGA AGCCTACGAG CGTCTGCTAA AGGTCAGAGA CGAGTTCATT
    CCGGTGGTGG TGGCAGCATC CAATCTCCGA GTCTACTCAT CGGTCACTCA CTGTGACATG
    AAACTTGGCT ACTCGCAGGA GGTAGAGAGC CATTACGTTG AAGGTCTGTG TAAACAGTTT
    TATGAAGACA TGGTGGACAT CATCCAGGCG ACGGTTCAGC AGAACTTTGA CACAGAGACA
    GATCCTCTCT ATGATGAAAT TATACAACAT TTGTCGCTGT GCAAGACGTT TTCATCTTTT
    TATGTGTACA AGAGCGAAGC CTTGGATATA GTCCAGGAGT ACCTGTACCC CTCGAAAGGT
    GGTCGAATAA CTCCCCAAGT GGTTTACGGA GGACCCTGCA CTGGAAAGAC CTTGTTACTT
    GCTGAAGTGG CAAAACAGGC CTATTCATGG ATACAGAAAG AGATGGGACC AGAAACAGAC
    CCAGTGGTCA TTGTCAGATT CATCGGTTCC ACTGACTTCT CTACTGACCT CCGCACCCTG
    CTGCAGAGCA TCTGTGAGCA GATTGCCATC AACTATCGGT GCCTGATCCA TTTCTTACCA
    AACAAGATCC AGGAGATGAG AGAACTGCTG GTGAATTTGC TGGGGGAGTC GTCATTCCAC
    CGACCTCTTG TCATTATTCT AGATGCCTTG GAGCAGTTGT CAGACGCAGA CGAGGCTCGA
    AAACTGTGGT GGCTCCCGGT CCACCTGCCA CGTACAGTTC GAATAGTAGT GTCCACCCTG
    CCCAACAAGC ACGGAATACT GCAGAAGCTG CGTTGCCTGA TACATGATGA GGAAGCCTAT
    GTTGAACTAA TGCAAAGAGA TCGCAAAGCT TGCAGTCAGA CCCTTAAACA GCAGCTTCTG
    GGTGTCAAAA GGAAGGTGAC GTCTGGCCAA CAGATATATG TTAATGAGGC AATGGCAAAA
    TGTACACTTC CTATGTTTGT TAACCTCATC TACAGAGAGG TTGTTCACTG GAGATCGCAC
    AAGGATGTGG ATGACAAATC GTTGTGTTCC ACAGTACACG AAAGTATTGA GCAGCTTTTC
    TACTCAGTTG AAAATAAGCT TGGCTCACGC TTTGTCTTTC GGGCACTGGG CTACATCACA
    ATGGCACGGG CAGGCCTGAC TGAGCTGGAG CTAGAGGATA TCTTATCTTT AGATAACAGT
    GTCCTAGGGG ACATCATGGT GAGCTCTAAT CTCAAAAGCC CACTGCGTAT TTCCTATGAT
    TTCATAGCTC GGCTAAAAGA AGAACTTGAA GGTTATTTAG TGGAGCGTCA GGTCCATAAT
    GTAACACTAA TGGTGTGGGC AAATCGGCAT CTACATCTCA TAGCACAGAA GCTGTACCTC
    AGTAACGAAG AAGACGTGCA TCAAATGCAT AGCCTTTTGG CTGAGTACTT TCTTGGGGCA
    TGGGCAGGTG GCAGAAAGAA AATATTCCAC TGCGACAATA ATCACTTTGC CTCATTGAAT
    ATCTCTCAGC ATCGAAATCC TCACAACCAA CAGAGCCTTC ATGGCCATGA CAAAGCTTCT
    GCTGACAAGC ACTCATATGA CAGACAGACA CCTGAGCAAC CCTGGGTGTT CCAATGTAAT
    CTGCTGGAGC CGGACATCTT CTTTGTCAAC CACCGTAAGA TGACAGAGCT CACATACCAC
    TTGACAAGGA GCGGCCGCAC AGATGATCTC ATGTATGGTG TCATCATGAA CTTCAGTTGG
    CTCTACACTA TGATAAAGAT AGGGCAATTT GACAAGGCCC TGTCTGACAT TGACCTAGCC
    TACAGCTATA CGCAAGAAAA GGAGCTAAAG TTTCTTGCAA CTACACTCCG CAGTATAAAG
    CTTAAAGTGA CAAAGAATCC AGCATCACTG TCTGCTGAAC TACAGCAAAG GCTCCTTCCA
    GTGGTCACAT CCCTTCCTAA ACTCAGACAC CTCCTGCTTG AGTGTGACAA GGACGGCCCT
    AAATACTGCT CCATCGTGCC ACTTCACTCC TCTATGGATG TGACCTACAG TCCAGAGAGA
    TTGCCGCTCA GTTCTAGCTA CATGCAGATA GTTGAGACTC TGCCTACATT AGCTCCAAAC
    ATAGTCATTG TGGCTCTTGA AGATGGGTCC GTCAGCACTT GGGATGTTGA AAGCAGACAG
    CTGCTAAGAC AGATAGACAC AGCAAGGTCA GTTGTTCTTG GAATTAGACT AACCACTGAT
    GAGAAATATC TGGTAGTGGC CACTACAAAA AATACACTGC TCATTTATGA CAACCACAAG
    TCTTGTTTGT TGTCTGAGGT AGAGGTCAAA GGGTCCAAAC ACTGTGGGAT CACTGGTGGG
    GTGGCATTCA TTAATGGATT TACCTTGTCC AGCCACCATG CCTTAGCTTG GCTTGAGGCA
    AGCAAAGATG TCAGTGTGAT TGATTTGCTG TACGGCTGGC CATTGTATCA GTTCCATTGC
    TGGTATGAGG TAACCTGTGT CCAGTGTTCC CCAGATGGAA TGTATGCTTT CTGTGGACAG
    TACCTCAACA CAACCACAAT ATTCCACCTT GGTAGTGGGG ATAAGCTTGC CACAATGACT
    TCTGAATTCT CAGGTGGTTT CGTGAAGTCC ATTCTGATTC TAGACACAAT CAATCAAATG
    GTGATGATTG ACAATGAGGG CAGTCTCTCC GTATGGAGCA CCAAAGATAT CACAAACCCC
    AAGCTAATGG AGGATTATGA CTGCAGAGGA GATGATAGTG AGGTGGTGGG CATAGAGCTC
    TCAGAGGATC AGCGATCCAT TCTAATCTGC AAAGCAAGAA GCATTGAGGT TCTGGACACC
    AAAATTTGGA AGATGGCTGA AAAGTTTAAG GCCAAGCGCA CTGAGAAGTT TGTGGCAGCT
    GTTCTCTCCA AGAATAGCCA AAGTATAATA GCCTCCATGG AGAACACATC ATCCATATTT
    GTGTGGAGGA GAGACAGTGG ACAGTGCATG GCCAGCTTGA TAGAGATATC GGGAGCCATT
    GTAAAGCTAA TCAAATCAAC ACACCATAAC TTGCTTCTGT CTGTGGCCAG CAGTGGGGTG
    CTTTCCGTGT GGGATATCGA CATTATAACA GCCATGTCGA ACATTGATAA AACTGGAAAA
    CCTATCCAAA TTCTGCAACT CTCTGGCAGA GAGGATTTTG TATACACCAT GGATGGGTCA
    GAAGTCATCC ATAAATGGAA CTACAGCACA GGTTTCATTG AAATGGTGTT CAAACATGAA
    GGACTTGTTG AGAACTGTGT CCTGACAACA TCAGGTGACC TAATGGTGAC ATCTGACGAT
    AAAAGTAGTC AGTACATCTG GCATACGACC ACAGGTGAGA ATATATTCCG TATCAATGGC
    CAGAAAATCT CACAGCTTCT CATCACGCAC AATGACCAAT TTGTTGTGTC ACTTTGTGAG
    CAGAATGCCT CTCGTGTCTG GAGGCTTGCC ACTGGTCACA AGGTGTGCAA CATTCTGGTA
    ACACTTCAAC ATGCCCTCAT TACTACAGCA AACACATTCC TTGTGGGTGC AAACAAGAAT
    AAGCTGCTGG CTGTTAGCTT ATGGTCAGGG AGTGTTTCCA AAAAGTTTAT CTGTGATGAT
    GGGATCACCA TTGTTAACTT CAAGTTAATC CCTGACAGTC CAGATTTTGT TGTCTTCATC
    ACCTCCACTG AAACGGTATT CATCTGGAGT GTTGCCGATG AGTCAGTATG TAGACGGGTG
    CAACTCCCCA ACAACTTCTT AAAGAACCTG GAAGATTTCC AAATTTCACC GAATGGGAAA
    TTGGGCATTG TCTCAAAAGG TGATGAGAAC ATCAATGTTC TAGACCTACA CAGTGGTAAG
    CTGCGTCTGG TGCATGCTGC TGGCATAATA TGGCGGCAAA AGCTTTCAAG AGATGGACGC
    TACTTAGTAT ACATCTGTTT CCGGAACTGC GATGAAGATG ATGATGCAGG TGTGGTTTCA
    AGCCTGATTG TCATGAGACT GGCAGATGGT AAGAGTATCG GGACCTGTTC CCTTTACAAA
    ACGCCAACCT ATCTGTGCCT ATCACAGCGA GCACTTAACA TCATTGTCGG ATTTGAGGAT
    GGCAGCATTG GCACTTATAC AGTGGTTGAT CGTGTAGATG CTGCTCTGAA GATCAAGATC
    GCCACCTCAA ACAGTCGCCA AATTGTTAAC AACGCCTCAC AGAAAGTACG ACCCAAGTGT
    AGCAACAATG CCTTCAAGAC CATTGCTGAC TGTGTGTGGA GAGAATCCAC TGAGGTCTTC
    TCTCGAGACA GTCCTATCAA CGTGTCTGAT GGTGGGGAGC CAGAGACCAC CACACCCACA
    AAGAAAACTG AACTGCTGCA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_690801.5
    CDS297..5540
    Translation

    Target ORF information:

    RefSeq Version XM_685709.8
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NACHT and WD repeat domain containing 2 (nwd2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_685709.8

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAAGAGGA TGAACCCACG GTGGAAGTCG CCACCCATGT GGCCATCCGG AGCGGGCAGC 
    AGGCAGCCCT GTCCCCGTGA ATCTGCACTG CGCAGAGCAG CCATCAGTGG CAACGTCCTT
    GCCCTCCCAC CATTCCATGT CCCCACGGGC AGGAGTGTCC GGGTCTTCAT CTGTGCCAAC
    CCTGACGATA CAGAAGCAGA GAGAAATGCT CTGAAAGAAC ATGTCTACCC CAAACTACGA
    GACTTCTGCC GAGAGAACTA TGGCATTGAA TTCCAGGTGG TGGACCTGTA CTGGGGCATT
    GATCCAGAGG AGTGGGACAG TCCGGAGCTG CAGAGACTGC GGATGAAACT ACTGGAAGAA
    TGTCTGAAGA CTTCAGCTGG TCCATGCTTT GTTGGGCTCA TAGGAGAGAA GTATGGCAGC
    ATTCGGGTCC CAGGGGAGGT GGAGTCAGCT GAGTTTGAGA TGATTTTGGA TGCGGCAGTG
    GAGGCGGGAC TAGACACACG GATCTTGGAG GAGTGGTACT GTCGAGATGA GAACTCAGTC
    CCACCTGCCT ACTACCTAAA ACCAAAAGCT GAGATGCTGA AAAACTATCA GAATTCTATG
    GAGTCAAACA CCGTAGCAAA ATCAAAGAAC GACAAGGCAT GGAGGGCAGT CTCAGAGGAA
    ATCAAGAGGA TCTTCAGAAC CTCTGTTTTA CAGCTGCAGG AGAAGGGGAC CATGAAGAGT
    GGTCAAGCCA AGAAATTTCT CTGCTCAGCA TTAGAGGATG AACTGGACTT CGCATTGGGC
    AAGCAGACCC CAGCCTTTCT CAAAAAATGT GTTTGTTACA TTCGGAAGAT ATCTAACTTT
    GATCGTTTCG CTAAACTCCC CGAAATGGCA CGTTACATGG ACATTGTGGT GAGCGATGAC
    CGTGTCATGA GAAACCAGGA AGCCTACGAG CGTCTGCTAA AGGTCAGAGA CGAGTTCATT
    CCGGTGGTGG TGGCAGCATC CAATCTCCGA GTCTACTCAT CGGTCACTCA CTGTGACATG
    AAACTTGGCT ACTCGCAGGA GGTAGAGAGC CATTACGTTG AAGGTCTGTG TAAACAGTTT
    TATGAAGACA TGGTGGACAT CATCCAGGCG ACGGTTCAGC AGAACTTTGA CACAGAGACA
    GATCCTCTCT ATGATGAAAT TATACAACAT TTGTCGCTGT GCAAGACGTT TTCATCTTTT
    TATGTGTACA AGAGCGAAGC CTTGGATATA GTCCAGGAGT ACCTGTACCC CTCGAAAGGT
    GGTCGAATAA CTCCCCAAGT GGTTTACGGA GGACCCTGCA CTGGAAAGAC CTTGTTACTT
    GCTGAAGTGG CAAAACAGGC CTATTCATGG ATACAGAAAG AGATGGGACC AGAAACAGAC
    CCAGTGGTCA TTGTCAGATT CATCGGTTCC ACTGACTTCT CTACTGACCT CCGCACCCTG
    CTGCAGAGCA TCTGTGAGCA GATTGCCATC AACTATCGGT GCCTGATCCA TTTCTTACCA
    AACAAGATCC AGGAGATGAG AGAACTGCTG GTGAATTTGC TGGGGGAGTC GTCATTCCAC
    CGACCTCTTG TCATTATTCT AGATGCCTTG GAGCAGTTGT CAGACGCAGA CGAGGCTCGA
    AAACTGTGGT GGCTCCCGGT CCACCTGCCA CGTACAGTTC GAATAGTAGT GTCCACCCTG
    CCCAACAAGC ACGGAATACT GCAGAAGCTG CGTTGCCTGA TACATGATGA GGAAGCCTAT
    GTTGAACTAA TGCAAAGAGA TCGCAAAGCT TGCAGTCAGA CCCTTAAACA GCAGCTTCTG
    GGTGTCAAAA GGAAGGTGAC GTCTGGCCAA CAGATATATG TTAATGAGGC AATGGCAAAA
    TGTACACTTC CTATGTTTGT TAACCTCATC TACAGAGAGG TTGTTCACTG GAGATCGCAC
    AAGGATGTGG ATGACAAATC GTTGTGTTCC ACAGTACACG AAAGTATTGA GCAGCTTTTC
    TACTCAGTTG AAAATAAGCT TGGCTCACGC TTTGTCTTTC GGGCACTGGG CTACATCACA
    ATGGCACGGG CAGGCCTGAC TGAGCTGGAG CTAGAGGATA TCTTATCTTT AGATAACAGT
    GTCCTAGGGG ACATCATGGT GAGCTCTAAT CTCAAAAGCC CACTGCGTAT TTCCTATGAT
    TTCATAGCTC GGCTAAAAGA AGAACTTGAA GGTTATTTAG TGGAGCGTCA GGTCCATAAT
    GTAACACTAA TGGTGTGGGC AAATCGGCAT CTACATCTCA TAGCACAGAA GCTGTACCTC
    AGTAACGAAG AAGACGTGCA TCAAATGCAT AGCCTTTTGG CTGAGTACTT TCTTGGGGCA
    TGGGCAGGTG GCAGAAAGAA AATATTCCAC TGCGACAATA ATCACTTTGC CTCATTGAAT
    ATCTCTCAGC ATCGAAATCC TCACAACCAA CAGAGCCTTC ATGGCCATGA CAAAGCTTCT
    GCTGACAAGC ACTCATATGA CAGACAGACA CCTGAGCAAC CCTGGGTGTT CCAATGTAAT
    CTGCTGGAGC CGGACATCTT CTTTGTCAAC CACCGTAAGA TGACAGAGCT CACATACCAC
    TTGACAAGGA GCGGCCGCAC AGATGATCTC ATGTATGGTG TCATCATGAA CTTCAGTTGG
    CTCTACACTA TGATAAAGAT AGGGCAATTT GACAAGGCCC TGTCTGACAT TGACCTAGCC
    TACAGCTATA CGCAAGAAAA GGAGCTAAAG TTTCTTGCAA CTACACTCCG CAGTATAAAG
    CTTAAAGTGA CAAAGAATCC AGCATCACTG TCTGCTGAAC TACAGCAAAG GCTCCTTCCA
    GTGGTCACAT CCCTTCCTAA ACTCAGACAC CTCCTGCTTG AGTGTGACAA GGACGGCCCT
    AAATACTGCT CCATCGTGCC ACTTCACTCC TCTATGGATG TGACCTACAG TCCAGAGAGA
    TTGCCGCTCA GTTCTAGCTA CATGCAGATA GTTGAGACTC TGCCTACATT AGCTCCAAAC
    ATAGTCATTG TGGCTCTTGA AGATGGGTCC GTCAGCACTT GGGATGTTGA AAGCAGACAG
    CTGCTAAGAC AGATAGACAC AGCAAGGTCA GTTGTTCTTG GAATTAGACT AACCACTGAT
    GAGAAATATC TGGTAGTGGC CACTACAAAA AATACACTGC TCATTTATGA CAACCACAAG
    TCTTGTTTGT TGTCTGAGGT AGAGGTCAAA GGGTCCAAAC ACTGTGGGAT CACTGGTGGG
    GTGGCATTCA TTAATGGATT TACCTTGTCC AGCCACCATG CCTTAGCTTG GCTTGAGGCA
    AGCAAAGATG TCAGTGTGAT TGATTTGCTG TACGGCTGGC CATTGTATCA GTTCCATTGC
    TGGTATGAGG TAACCTGTGT CCAGTGTTCC CCAGATGGAA TGTATGCTTT CTGTGGACAG
    TACCTCAACA CAACCACAAT ATTCCACCTT GGTAGTGGGG ATAAGCTTGC CACAATGACT
    TCTGAATTCT CAGGTGGTTT CGTGAAGTCC ATTCTGATTC TAGACACAAT CAATCAAATG
    GTGATGATTG ACAATGAGGG CAGTCTCTCC GTATGGAGCA CCAAAGATAT CACAAACCCC
    AAGCTAATGG AGGATTATGA CTGCAGAGGA GATGATAGTG AGGTGGTGGG CATAGAGCTC
    TCAGAGGATC AGCGATCCAT TCTAATCTGC AAAGCAAGAA GCATTGAGGT TCTGGACACC
    AAAATTTGGA AGATGGCTGA AAAGTTTAAG GCCAAGCGCA CTGAGAAGTT TGTGGCAGCT
    GTTCTCTCCA AGAATAGCCA AAGTATAATA GCCTCCATGG AGAACACATC ATCCATATTT
    GTGTGGAGGA GAGACAGTGG ACAGTGCATG GCCAGCTTGA TAGAGATATC GGGAGCCATT
    GTAAAGCTAA TCAAATCAAC ACACCATAAC TTGCTTCTGT CTGTGGCCAG CAGTGGGGTG
    CTTTCCGTGT GGGATATCGA CATTATAACA GCCATGTCGA ACATTGATAA AACTGGAAAA
    CCTATCCAAA TTCTGCAACT CTCTGGCAGA GAGGATTTTG TATACACCAT GGATGGGTCA
    GAAGTCATCC ATAAATGGAA CTACAGCACA GGTTTCATTG AAATGGTGTT CAAACATGAA
    GGACTTGTTG AGAACTGTGT CCTGACAACA TCAGGTGACC TAATGGTGAC ATCTGACGAT
    AAAAGTAGTC AGTACATCTG GCATACGACC ACAGGTGAGA ATATATTCCG TATCAATGGC
    CAGAAAATCT CACAGCTTCT CATCACGCAC AATGACCAAT TTGTTGTGTC ACTTTGTGAG
    CAGAATGCCT CTCGTGTCTG GAGGCTTGCC ACTGGTCACA AGGTGTGCAA CATTCTGGTA
    ACACTTCAAC ATGCCCTCAT TACTACAGCA AACACATTCC TTGTGGGTGC AAACAAGAAT
    AAGCTGCTGG CTGTTAGCTT ATGGTCAGGG AGTGTTTCCA AAAAGTTTAT CTGTGATGAT
    GGGATCACCA TTGTTAACTT CAAGTTAATC CCTGACAGTC CAGATTTTGT TGTCTTCATC
    ACCTCCACTG AAACGGTATT CATCTGGAGT GTTGCCGATG AGTCAGTATG TAGACGGGTG
    CAACTCCCCA ACAACTTCTT AAAGAACCTG GAAGATTTCC AAATTTCACC GAATGGGAAA
    TTGGGCATTG TCTCAAAAGG TGATGAGAAC ATCAATGTTC TAGACCTACA CAGTGGTAAG
    CTGCGTCTGG TGCATGCTGC TGGCATAATA TGGCGGCAAA AGCTTTCAAG AGATGGACGC
    TACTTAGTAT ACATCTGTTT CCGGAACTGC GATGAAGATG ATGATGCAGG TGTGGTTTCA
    AGCCTGATTG TCATGAGACT GGCAGATGGT AAGAGTATCG GGACCTGTTC CCTTTACAAA
    ACGCCAACCT ATCTGTGCCT ATCACAGCGA GCACTTAACA TCATTGTCGG ATTTGAGGAT
    GGCAGCATTG GCACTTATAC AGTGGTTGAT CGTGTAGATG CTGCTCTGAA GATCAAGATC
    GCCACCTCAA ACAGTCGCCA AATTGTTAAC AACGCCTCAC AGAAAGTACG ACCCAAGTGT
    AGCAACAATG CCTTCAAGAC CATTGCTGAC TGTGTGTGGA GAGAATCCAC TGAGGTCTTC
    TCTCGAGACA GTCCTATCAA CGTGTCTGAT GGTGGGGAGC CAGAGACCAC CACACCCACA
    AAGAAAACTG AACTGCTGCA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13555
    Clone ID Related Accession (Same CDS sequence) XM_685709.8 , XM_685709.7
    Accession Version XM_685709.7 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5244bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product NACHT and WD repeat domain-containing protein 2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_685709.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAAGAGGA TGAACCCACG GTGGAAGTCG CCACCCATGT GGCCATCCGG AGCGGGCAGC 
    AGGCAGCCCT GTCCCCGTGA ATCTGCACTG CGCAGAGCAG CCATCAGTGG CAACGTCCTT
    GCCCTCCCAC CATTCCATGT CCCCACGGGC AGGAGTGTCC GGGTCTTCAT CTGTGCCAAC
    CCTGACGATA CAGAAGCAGA GAGAAATGCT CTGAAAGAAC ATGTCTACCC CAAACTACGA
    GACTTCTGCC GAGAGAACTA TGGCATTGAA TTCCAGGTGG TGGACCTGTA CTGGGGCATT
    GATCCAGAGG AGTGGGACAG TCCGGAGCTG CAGAGACTGC GGATGAAACT ACTGGAAGAA
    TGTCTGAAGA CTTCAGCTGG TCCATGCTTT GTTGGGCTCA TAGGAGAGAA GTATGGCAGC
    ATTCGGGTCC CAGGGGAGGT GGAGTCAGCT GAGTTTGAGA TGATTTTGGA TGCGGCAGTG
    GAGGCGGGAC TAGACACACG GATCTTGGAG GAGTGGTACT GTCGAGATGA GAACTCAGTC
    CCACCTGCCT ACTACCTAAA ACCAAAAGCT GAGATGCTGA AAAACTATCA GAATTCTATG
    GAGTCAAACA CCGTAGCAAA ATCAAAGAAC GACAAGGCAT GGAGGGCAGT CTCAGAGGAA
    ATCAAGAGGA TCTTCAGAAC CTCTGTTTTA CAGCTGCAGG AGAAGGGGAC CATGAAGAGT
    GGTCAAGCCA AGAAATTTCT CTGCTCAGCA TTAGAGGATG AACTGGACTT CGCATTGGGC
    AAGCAGACCC CAGCCTTTCT CAAAAAATGT GTTTGTTACA TTCGGAAGAT ATCTAACTTT
    GATCGTTTCG CTAAACTCCC CGAAATGGCA CGTTACATGG ACATTGTGGT GAGCGATGAC
    CGTGTCATGA GAAACCAGGA AGCCTACGAG CGTCTGCTAA AGGTCAGAGA CGAGTTCATT
    CCGGTGGTGG TGGCAGCATC CAATCTCCGA GTCTACTCAT CGGTCACTCA CTGTGACATG
    AAACTTGGCT ACTCGCAGGA GGTAGAGAGC CATTACGTTG AAGGTCTGTG TAAACAGTTT
    TATGAAGACA TGGTGGACAT CATCCAGGCG ACGGTTCAGC AGAACTTTGA CACAGAGACA
    GATCCTCTCT ATGATGAAAT TATACAACAT TTGTCGCTGT GCAAGACGTT TTCATCTTTT
    TATGTGTACA AGAGCGAAGC CTTGGATATA GTCCAGGAGT ACCTGTACCC CTCGAAAGGT
    GGTCGAATAA CTCCCCAAGT GGTTTACGGA GGACCCTGCA CTGGAAAGAC CTTGTTACTT
    GCTGAAGTGG CAAAACAGGC CTATTCATGG ATACAGAAAG AGATGGGACC AGAAACAGAC
    CCAGTGGTCA TTGTCAGATT CATCGGTTCC ACTGACTTCT CTACTGACCT CCGCACCCTG
    CTGCAGAGCA TCTGTGAGCA GATTGCCATC AACTATCGGT GCCTGATCCA TTTCTTACCA
    AACAAGATCC AGGAGATGAG AGAACTGCTG GTGAATTTGC TGGGGGAGTC GTCATTCCAC
    CGACCTCTTG TCATTATTCT AGATGCCTTG GAGCAGTTGT CAGACGCAGA CGAGGCTCGA
    AAACTGTGGT GGCTCCCGGT CCACCTGCCA CGTACAGTTC GAATAGTAGT GTCCACCCTG
    CCCAACAAGC ACGGAATACT GCAGAAGCTG CGTTGCCTGA TACATGATGA GGAAGCCTAT
    GTTGAACTAA TGCAAAGAGA TCGCAAAGCT TGCAGTCAGA CCCTTAAACA GCAGCTTCTG
    GGTGTCAAAA GGAAGGTGAC GTCTGGCCAA CAGATATATG TTAATGAGGC AATGGCAAAA
    TGTACACTTC CTATGTTTGT TAACCTCATC TACAGAGAGG TTGTTCACTG GAGATCGCAC
    AAGGATGTGG ATGACAAATC GTTGTGTTCC ACAGTACACG AAAGTATTGA GCAGCTTTTC
    TACTCAGTTG AAAATAAGCT TGGCTCACGC TTTGTCTTTC GGGCACTGGG CTACATCACA
    ATGGCACGGG CAGGCCTGAC TGAGCTGGAG CTAGAGGATA TCTTATCTTT AGATAACAGT
    GTCCTAGGGG ACATCATGGT GAGCTCTAAT CTCAAAAGCC CACTGCGTAT TTCCTATGAT
    TTCATAGCTC GGCTAAAAGA AGAACTTGAA GGTTATTTAG TGGAGCGTCA GGTCCATAAT
    GTAACACTAA TGGTGTGGGC AAATCGGCAT CTACATCTCA TAGCACAGAA GCTGTACCTC
    AGTAACGAAG AAGACGTGCA TCAAATGCAT AGCCTTTTGG CTGAGTACTT TCTTGGGGCA
    TGGGCAGGTG GCAGAAAGAA AATATTCCAC TGCGACAATA ATCACTTTGC CTCATTGAAT
    ATCTCTCAGC ATCGAAATCC TCACAACCAA CAGAGCCTTC ATGGCCATGA CAAAGCTTCT
    GCTGACAAGC ACTCATATGA CAGACAGACA CCTGAGCAAC CCTGGGTGTT CCAATGTAAT
    CTGCTGGAGC CGGACATCTT CTTTGTCAAC CACCGTAAGA TGACAGAGCT CACATACCAC
    TTGACAAGGA GCGGCCGCAC AGATGATCTC ATGTATGGTG TCATCATGAA CTTCAGTTGG
    CTCTACACTA TGATAAAGAT AGGGCAATTT GACAAGGCCC TGTCTGACAT TGACCTAGCC
    TACAGCTATA CGCAAGAAAA GGAGCTAAAG TTTCTTGCAA CTACACTCCG CAGTATAAAG
    CTTAAAGTGA CAAAGAATCC AGCATCACTG TCTGCTGAAC TACAGCAAAG GCTCCTTCCA
    GTGGTCACAT CCCTTCCTAA ACTCAGACAC CTCCTGCTTG AGTGTGACAA GGACGGCCCT
    AAATACTGCT CCATCGTGCC ACTTCACTCC TCTATGGATG TGACCTACAG TCCAGAGAGA
    TTGCCGCTCA GTTCTAGCTA CATGCAGATA GTTGAGACTC TGCCTACATT AGCTCCAAAC
    ATAGTCATTG TGGCTCTTGA AGATGGGTCC GTCAGCACTT GGGATGTTGA AAGCAGACAG
    CTGCTAAGAC AGATAGACAC AGCAAGGTCA GTTGTTCTTG GAATTAGACT AACCACTGAT
    GAGAAATATC TGGTAGTGGC CACTACAAAA AATACACTGC TCATTTATGA CAACCACAAG
    TCTTGTTTGT TGTCTGAGGT AGAGGTCAAA GGGTCCAAAC ACTGTGGGAT CACTGGTGGG
    GTGGCATTCA TTAATGGATT TACCTTGTCC AGCCACCATG CCTTAGCTTG GCTTGAGGCA
    AGCAAAGATG TCAGTGTGAT TGATTTGCTG TACGGCTGGC CATTGTATCA GTTCCATTGC
    TGGTATGAGG TAACCTGTGT CCAGTGTTCC CCAGATGGAA TGTATGCTTT CTGTGGACAG
    TACCTCAACA CAACCACAAT ATTCCACCTT GGTAGTGGGG ATAAGCTTGC CACAATGACT
    TCTGAATTCT CAGGTGGTTT CGTGAAGTCC ATTCTGATTC TAGACACAAT CAATCAAATG
    GTGATGATTG ACAATGAGGG CAGTCTCTCC GTATGGAGCA CCAAAGATAT CACAAACCCC
    AAGCTAATGG AGGATTATGA CTGCAGAGGA GATGATAGTG AGGTGGTGGG CATAGAGCTC
    TCAGAGGATC AGCGATCCAT TCTAATCTGC AAAGCAAGAA GCATTGAGGT TCTGGACACC
    AAAATTTGGA AGATGGCTGA AAAGTTTAAG GCCAAGCGCA CTGAGAAGTT TGTGGCAGCT
    GTTCTCTCCA AGAATAGCCA AAGTATAATA GCCTCCATGG AGAACACATC ATCCATATTT
    GTGTGGAGGA GAGACAGTGG ACAGTGCATG GCCAGCTTGA TAGAGATATC GGGAGCCATT
    GTAAAGCTAA TCAAATCAAC ACACCATAAC TTGCTTCTGT CTGTGGCCAG CAGTGGGGTG
    CTTTCCGTGT GGGATATCGA CATTATAACA GCCATGTCGA ACATTGATAA AACTGGAAAA
    CCTATCCAAA TTCTGCAACT CTCTGGCAGA GAGGATTTTG TATACACCAT GGATGGGTCA
    GAAGTCATCC ATAAATGGAA CTACAGCACA GGTTTCATTG AAATGGTGTT CAAACATGAA
    GGACTTGTTG AGAACTGTGT CCTGACAACA TCAGGTGACC TAATGGTGAC ATCTGACGAT
    AAAAGTAGTC AGTACATCTG GCATACGACC ACAGGTGAGA ATATATTCCG TATCAATGGC
    CAGAAAATCT CACAGCTTCT CATCACGCAC AATGACCAAT TTGTTGTGTC ACTTTGTGAG
    CAGAATGCCT CTCGTGTCTG GAGGCTTGCC ACTGGTCACA AGGTGTGCAA CATTCTGGTA
    ACACTTCAAC ATGCCCTCAT TACTACAGCA AACACATTCC TTGTGGGTGC AAACAAGAAT
    AAGCTGCTGG CTGTTAGCTT ATGGTCAGGG AGTGTTTCCA AAAAGTTTAT CTGTGATGAT
    GGGATCACCA TTGTTAACTT CAAGTTAATC CCTGACAGTC CAGATTTTGT TGTCTTCATC
    ACCTCCACTG AAACGGTATT CATCTGGAGT GTTGCCGATG AGTCAGTATG TAGACGGGTG
    CAACTCCCCA ACAACTTCTT AAAGAACCTG GAAGATTTCC AAATTTCACC GAATGGGAAA
    TTGGGCATTG TCTCAAAAGG TGATGAGAAC ATCAATGTTC TAGACCTACA CAGTGGTAAG
    CTGCGTCTGG TGCATGCTGC TGGCATAATA TGGCGGCAAA AGCTTTCAAG AGATGGACGC
    TACTTAGTAT ACATCTGTTT CCGGAACTGC GATGAAGATG ATGATGCAGG TGTGGTTTCA
    AGCCTGATTG TCATGAGACT GGCAGATGGT AAGAGTATCG GGACCTGTTC CCTTTACAAA
    ACGCCAACCT ATCTGTGCCT ATCACAGCGA GCACTTAACA TCATTGTCGG ATTTGAGGAT
    GGCAGCATTG GCACTTATAC AGTGGTTGAT CGTGTAGATG CTGCTCTGAA GATCAAGATC
    GCCACCTCAA ACAGTCGCCA AATTGTTAAC AACGCCTCAC AGAAAGTACG ACCCAAGTGT
    AGCAACAATG CCTTCAAGAC CATTGCTGAC TGTGTGTGGA GAGAATCCAC TGAGGTCTTC
    TCTCGAGACA GTCCTATCAA CGTGTCTGAT GGTGGGGAGC CAGAGACCAC CACACCCACA
    AAGAAAACTG AACTGCTGCA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_690801.5
    CDS270..5513
    Translation

    Target ORF information:

    RefSeq Version XM_685709.7
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio NACHT and WD repeat domain containing 2 (nwd2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_685709.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    ATGAAGAGGA TGAACCCACG GTGGAAGTCG CCACCCATGT GGCCATCCGG AGCGGGCAGC 
    AGGCAGCCCT GTCCCCGTGA ATCTGCACTG CGCAGAGCAG CCATCAGTGG CAACGTCCTT
    GCCCTCCCAC CATTCCATGT CCCCACGGGC AGGAGTGTCC GGGTCTTCAT CTGTGCCAAC
    CCTGACGATA CAGAAGCAGA GAGAAATGCT CTGAAAGAAC ATGTCTACCC CAAACTACGA
    GACTTCTGCC GAGAGAACTA TGGCATTGAA TTCCAGGTGG TGGACCTGTA CTGGGGCATT
    GATCCAGAGG AGTGGGACAG TCCGGAGCTG CAGAGACTGC GGATGAAACT ACTGGAAGAA
    TGTCTGAAGA CTTCAGCTGG TCCATGCTTT GTTGGGCTCA TAGGAGAGAA GTATGGCAGC
    ATTCGGGTCC CAGGGGAGGT GGAGTCAGCT GAGTTTGAGA TGATTTTGGA TGCGGCAGTG
    GAGGCGGGAC TAGACACACG GATCTTGGAG GAGTGGTACT GTCGAGATGA GAACTCAGTC
    CCACCTGCCT ACTACCTAAA ACCAAAAGCT GAGATGCTGA AAAACTATCA GAATTCTATG
    GAGTCAAACA CCGTAGCAAA ATCAAAGAAC GACAAGGCAT GGAGGGCAGT CTCAGAGGAA
    ATCAAGAGGA TCTTCAGAAC CTCTGTTTTA CAGCTGCAGG AGAAGGGGAC CATGAAGAGT
    GGTCAAGCCA AGAAATTTCT CTGCTCAGCA TTAGAGGATG AACTGGACTT CGCATTGGGC
    AAGCAGACCC CAGCCTTTCT CAAAAAATGT GTTTGTTACA TTCGGAAGAT ATCTAACTTT
    GATCGTTTCG CTAAACTCCC CGAAATGGCA CGTTACATGG ACATTGTGGT GAGCGATGAC
    CGTGTCATGA GAAACCAGGA AGCCTACGAG CGTCTGCTAA AGGTCAGAGA CGAGTTCATT
    CCGGTGGTGG TGGCAGCATC CAATCTCCGA GTCTACTCAT CGGTCACTCA CTGTGACATG
    AAACTTGGCT ACTCGCAGGA GGTAGAGAGC CATTACGTTG AAGGTCTGTG TAAACAGTTT
    TATGAAGACA TGGTGGACAT CATCCAGGCG ACGGTTCAGC AGAACTTTGA CACAGAGACA
    GATCCTCTCT ATGATGAAAT TATACAACAT TTGTCGCTGT GCAAGACGTT TTCATCTTTT
    TATGTGTACA AGAGCGAAGC CTTGGATATA GTCCAGGAGT ACCTGTACCC CTCGAAAGGT
    GGTCGAATAA CTCCCCAAGT GGTTTACGGA GGACCCTGCA CTGGAAAGAC CTTGTTACTT
    GCTGAAGTGG CAAAACAGGC CTATTCATGG ATACAGAAAG AGATGGGACC AGAAACAGAC
    CCAGTGGTCA TTGTCAGATT CATCGGTTCC ACTGACTTCT CTACTGACCT CCGCACCCTG
    CTGCAGAGCA TCTGTGAGCA GATTGCCATC AACTATCGGT GCCTGATCCA TTTCTTACCA
    AACAAGATCC AGGAGATGAG AGAACTGCTG GTGAATTTGC TGGGGGAGTC GTCATTCCAC
    CGACCTCTTG TCATTATTCT AGATGCCTTG GAGCAGTTGT CAGACGCAGA CGAGGCTCGA
    AAACTGTGGT GGCTCCCGGT CCACCTGCCA CGTACAGTTC GAATAGTAGT GTCCACCCTG
    CCCAACAAGC ACGGAATACT GCAGAAGCTG CGTTGCCTGA TACATGATGA GGAAGCCTAT
    GTTGAACTAA TGCAAAGAGA TCGCAAAGCT TGCAGTCAGA CCCTTAAACA GCAGCTTCTG
    GGTGTCAAAA GGAAGGTGAC GTCTGGCCAA CAGATATATG TTAATGAGGC AATGGCAAAA
    TGTACACTTC CTATGTTTGT TAACCTCATC TACAGAGAGG TTGTTCACTG GAGATCGCAC
    AAGGATGTGG ATGACAAATC GTTGTGTTCC ACAGTACACG AAAGTATTGA GCAGCTTTTC
    TACTCAGTTG AAAATAAGCT TGGCTCACGC TTTGTCTTTC GGGCACTGGG CTACATCACA
    ATGGCACGGG CAGGCCTGAC TGAGCTGGAG CTAGAGGATA TCTTATCTTT AGATAACAGT
    GTCCTAGGGG ACATCATGGT GAGCTCTAAT CTCAAAAGCC CACTGCGTAT TTCCTATGAT
    TTCATAGCTC GGCTAAAAGA AGAACTTGAA GGTTATTTAG TGGAGCGTCA GGTCCATAAT
    GTAACACTAA TGGTGTGGGC AAATCGGCAT CTACATCTCA TAGCACAGAA GCTGTACCTC
    AGTAACGAAG AAGACGTGCA TCAAATGCAT AGCCTTTTGG CTGAGTACTT TCTTGGGGCA
    TGGGCAGGTG GCAGAAAGAA AATATTCCAC TGCGACAATA ATCACTTTGC CTCATTGAAT
    ATCTCTCAGC ATCGAAATCC TCACAACCAA CAGAGCCTTC ATGGCCATGA CAAAGCTTCT
    GCTGACAAGC ACTCATATGA CAGACAGACA CCTGAGCAAC CCTGGGTGTT CCAATGTAAT
    CTGCTGGAGC CGGACATCTT CTTTGTCAAC CACCGTAAGA TGACAGAGCT CACATACCAC
    TTGACAAGGA GCGGCCGCAC AGATGATCTC ATGTATGGTG TCATCATGAA CTTCAGTTGG
    CTCTACACTA TGATAAAGAT AGGGCAATTT GACAAGGCCC TGTCTGACAT TGACCTAGCC
    TACAGCTATA CGCAAGAAAA GGAGCTAAAG TTTCTTGCAA CTACACTCCG CAGTATAAAG
    CTTAAAGTGA CAAAGAATCC AGCATCACTG TCTGCTGAAC TACAGCAAAG GCTCCTTCCA
    GTGGTCACAT CCCTTCCTAA ACTCAGACAC CTCCTGCTTG AGTGTGACAA GGACGGCCCT
    AAATACTGCT CCATCGTGCC ACTTCACTCC TCTATGGATG TGACCTACAG TCCAGAGAGA
    TTGCCGCTCA GTTCTAGCTA CATGCAGATA GTTGAGACTC TGCCTACATT AGCTCCAAAC
    ATAGTCATTG TGGCTCTTGA AGATGGGTCC GTCAGCACTT GGGATGTTGA AAGCAGACAG
    CTGCTAAGAC AGATAGACAC AGCAAGGTCA GTTGTTCTTG GAATTAGACT AACCACTGAT
    GAGAAATATC TGGTAGTGGC CACTACAAAA AATACACTGC TCATTTATGA CAACCACAAG
    TCTTGTTTGT TGTCTGAGGT AGAGGTCAAA GGGTCCAAAC ACTGTGGGAT CACTGGTGGG
    GTGGCATTCA TTAATGGATT TACCTTGTCC AGCCACCATG CCTTAGCTTG GCTTGAGGCA
    AGCAAAGATG TCAGTGTGAT TGATTTGCTG TACGGCTGGC CATTGTATCA GTTCCATTGC
    TGGTATGAGG TAACCTGTGT CCAGTGTTCC CCAGATGGAA TGTATGCTTT CTGTGGACAG
    TACCTCAACA CAACCACAAT ATTCCACCTT GGTAGTGGGG ATAAGCTTGC CACAATGACT
    TCTGAATTCT CAGGTGGTTT CGTGAAGTCC ATTCTGATTC TAGACACAAT CAATCAAATG
    GTGATGATTG ACAATGAGGG CAGTCTCTCC GTATGGAGCA CCAAAGATAT CACAAACCCC
    AAGCTAATGG AGGATTATGA CTGCAGAGGA GATGATAGTG AGGTGGTGGG CATAGAGCTC
    TCAGAGGATC AGCGATCCAT TCTAATCTGC AAAGCAAGAA GCATTGAGGT TCTGGACACC
    AAAATTTGGA AGATGGCTGA AAAGTTTAAG GCCAAGCGCA CTGAGAAGTT TGTGGCAGCT
    GTTCTCTCCA AGAATAGCCA AAGTATAATA GCCTCCATGG AGAACACATC ATCCATATTT
    GTGTGGAGGA GAGACAGTGG ACAGTGCATG GCCAGCTTGA TAGAGATATC GGGAGCCATT
    GTAAAGCTAA TCAAATCAAC ACACCATAAC TTGCTTCTGT CTGTGGCCAG CAGTGGGGTG
    CTTTCCGTGT GGGATATCGA CATTATAACA GCCATGTCGA ACATTGATAA AACTGGAAAA
    CCTATCCAAA TTCTGCAACT CTCTGGCAGA GAGGATTTTG TATACACCAT GGATGGGTCA
    GAAGTCATCC ATAAATGGAA CTACAGCACA GGTTTCATTG AAATGGTGTT CAAACATGAA
    GGACTTGTTG AGAACTGTGT CCTGACAACA TCAGGTGACC TAATGGTGAC ATCTGACGAT
    AAAAGTAGTC AGTACATCTG GCATACGACC ACAGGTGAGA ATATATTCCG TATCAATGGC
    CAGAAAATCT CACAGCTTCT CATCACGCAC AATGACCAAT TTGTTGTGTC ACTTTGTGAG
    CAGAATGCCT CTCGTGTCTG GAGGCTTGCC ACTGGTCACA AGGTGTGCAA CATTCTGGTA
    ACACTTCAAC ATGCCCTCAT TACTACAGCA AACACATTCC TTGTGGGTGC AAACAAGAAT
    AAGCTGCTGG CTGTTAGCTT ATGGTCAGGG AGTGTTTCCA AAAAGTTTAT CTGTGATGAT
    GGGATCACCA TTGTTAACTT CAAGTTAATC CCTGACAGTC CAGATTTTGT TGTCTTCATC
    ACCTCCACTG AAACGGTATT CATCTGGAGT GTTGCCGATG AGTCAGTATG TAGACGGGTG
    CAACTCCCCA ACAACTTCTT AAAGAACCTG GAAGATTTCC AAATTTCACC GAATGGGAAA
    TTGGGCATTG TCTCAAAAGG TGATGAGAAC ATCAATGTTC TAGACCTACA CAGTGGTAAG
    CTGCGTCTGG TGCATGCTGC TGGCATAATA TGGCGGCAAA AGCTTTCAAG AGATGGACGC
    TACTTAGTAT ACATCTGTTT CCGGAACTGC GATGAAGATG ATGATGCAGG TGTGGTTTCA
    AGCCTGATTG TCATGAGACT GGCAGATGGT AAGAGTATCG GGACCTGTTC CCTTTACAAA
    ACGCCAACCT ATCTGTGCCT ATCACAGCGA GCACTTAACA TCATTGTCGG ATTTGAGGAT
    GGCAGCATTG GCACTTATAC AGTGGTTGAT CGTGTAGATG CTGCTCTGAA GATCAAGATC
    GCCACCTCAA ACAGTCGCCA AATTGTTAAC AACGCCTCAC AGAAAGTACG ACCCAAGTGT
    AGCAACAATG CCTTCAAGAC CATTGCTGAC TGTGTGTGGA GAGAATCCAC TGAGGTCTTC
    TCTCGAGACA GTCCTATCAA CGTGTCTGAT GGTGGGGAGC CAGAGACCAC CACACCCACA
    AAGAAAACTG AACTGCTGCA ATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13556
    Clone ID Related Accession (Same CDS sequence) XM_005172360.2 , XM_005172360.3
    Accession Version XM_005172360.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product NACHT and WD repeat domain-containing protein 2 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_005172360.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATTTTGG ATGCGGCAGT GGAGGCGGGA CTAGACACAC GGATCTTGGA GGAGTGGTAC 
    TGTCGAGATG AGAACTCAGT CCCACCTGCC TACTACCTAA AACCAAAAGC TGAGATGCTG
    AAAAACTATC AGAATTCTAT GGAGTCAAAC ACCGTAGCAA AATCAAAGAA CGACAAGGCA
    TGGAGGGCAG TCTCAGAGGA AATCAAGAGG ATCTTCAGAA CCTCTGTTTT ACAGCTGCAG
    GAGAAGGGGA CCATGAAGAG TGGTCAAGCC AAGAAATTTC TCTGCTCAGC ATTAGAGGAT
    GAACTGGACT TCGCATTGGG CAAGCAGACC CCAGCCTTTC TCAAAAAATG TGTTTGTTAC
    ATTCGGAAGA TATCTAACTT TGATCGTTTC GCTAAACTCC CCGAAATGGC ACGTTACATG
    GACATTGTGG TGAGCGATGA CCGTGTCATG AGAAACCAGG AAGCCTACGA GCGTCTGCTA
    AAGGTCAGAG ACGAGTTCAT TCCGGTGGTG GTGGCAGCAT CCAATCTCCG AGTCTACTCA
    TCGGTCACTC ACTGTGACAT GAAACTTGGC TACTCGCAGG AGGTAGAGAG CCATTACGTT
    GAAGGTCTGT GTAAACAGTT TTATGAAGAC ATGGTGGACA TCATCCAGGC GACGGTTCAG
    CAGAACTTTG ACACAGAGAC AGATCCTCTC TATGATGAAA TTATACAACA TTTGTCGCTG
    TGCAAGACGT TTTCATCTTT TTATGTGTAC AAGAGCGAAG CCTTGGATAT AGTCCAGGAG
    TACCTGTACC CCTCGAAAGG TGGTCGAATA ACTCCCCAAG TGGTTTACGG AGGACCCTGC
    ACTGGAAAGA CCTTGTTACT TGCTGAAGTG GCAAAACAGG CCTATTCATG GATACAGAAA
    GAGATGGGAC CAGAAACAGA CCCAGTGGTC ATTGTCAGAT TCATCGGTTC CACTGACTTC
    TCTACTGACC TCCGCACCCT GCTGCAGAGC ATCTGTGAGC AGATTGCCAT CAACTATCGG
    TGCCTGATCC ATTTCTTACC AAACAAGATC CAGGAGATGA GAGAACTGCT GGTGAATTTG
    CTGGGGGAGT CGTCATTCCA CCGACCTCTT GTCATTATTC TAGATGCCTT GGAGCAGTTG
    TCAGACGCAG ACGAGGCTCG AAAACTGTGG TGGCTCCCGG TCCACCTGCC ACGTACAGTT
    CGAATAGTAG TGTCCACCCT GCCCAACAAG CACGGAATAC TGCAGAAGCT GCGTTGCCTG
    ATACATGATG AGGAAGCCTA TGTTGAACTA ATGCAAAGAG ATCGCAAAGC TTGCAGTCAG
    ACCCTTAAAC AGCAGCTTCT GGGTGTCAAA AGGAAGGTGA CGTCTGGCCA ACAGATATAT
    GTTAATGAGG CAATGGCAAA ATGTACACTT CCTATGTTTG TTAACCTCAT CTACAGAGAG
    GTTGTTCACT GGAGATCGCA CAAGGATGTG GATGACAAAT CGTTGTGTTC CACAGTACAC
    GAAAGTATTG AGCAGCTTTT CTACTCAGTT GAAAATAAGC TTGGCTCACG CTTTGTCTTT
    CGGGCACTGG GCTACATCAC AATGGCACGG GCAGGCCTGA CTGAGCTGGA GCTAGAGGAT
    ATCTTATCTT TAGATAACAG TGTCCTAGGG GACATCATGG TGAGCTCTAA TCTCAAAAGC
    CCACTGCGTA TTTCCTATGA TTTCATAGCT CGGCTAAAAG AAGAACTTGA AGGTTATTTA
    GTGGAGCGTC AGGTCCATAA TGTAACACTA ATGGTGTGGG CAAATCGGCA TCTACATCTC
    ATAGCACAGA AGCTGTACCT CAGTAACGAA GAAGACGTGC ATCAAATGCA TAGCCTTTTG
    GCTGAGTACT TTCTTGGGGC ATGGGCAGGT GGCAGAAAGA AAATATTCCA CTGCGACAAT
    AATCACTTTG CCTCATTGAA TATCTCTCAG CATCGAAATC CTCACAACCA ACAGAGCCTT
    CATGGCCATG ACAAAGCTTC TGCTGACAAG CACTCATATG ACAGACAGAC ACCTGAGCAA
    CCCTGGGTGT TCCAATGTAA TCTGCTGGAG CCGGACATCT TCTTTGTCAA CCACCGTAAG
    ATGACAGAGC TCACATACCA CTTGACAAGG AGCGGCCGCA CAGATGATCT CATGTATGGT
    GTCATCATGA ACTTCAGTTG GCTCTACACT ATGATAAAGA TAGGGCAATT TGACAAGGCC
    CTGTCTGACA TTGACCTAGC CTACAGCTAT ACGCAAGAAA AGGAGCTAAA GTTTCTTGCA
    ACTACACTCC GCAGTATAAA GCTTAAAGTG ACAAAGAATC CAGCATCACT GTCTGCTGAA
    CTACAGCAAA GGCTCCTTCC AGTGGTCACA TCCCTTCCTA AACTCAGACA CCTCCTGCTT
    GAGTGTGACA AGGACGGCCC TAAATACTGC TCCATCGTGC CACTTCACTC CTCTATGGAT
    GTGACCTACA GTCCAGAGAG ATTGCCGCTC AGTTCTAGCT ACATGCAGAT AGTTGAGACT
    CTGCCTACAT TAGCTCCAAA CATAGTCATT GTGGCTCTTG AAGATGGGTC CGTCAGCACT
    TGGGATGTTG AAAGCAGACA GCTGCTAAGA CAGATAGACA CAGCAAGGTC AGTTGTTCTT
    GGAATTAGAC TAACCACTGA TGAGAAATAT CTGGTAGTGG CCACTACAAA AAATACACTG
    CTCATTTATG ACAACCACAA GTCTTGTTTG TTGTCTGAGG TAGAGGTCAA AGGGTCCAAA
    CACTGTGGGA TCACTGGTGG GGTGGCATTC ATTAATGGAT TTACCTTGTC CAGCCACCAT
    GCCTTAGCTT GGCTTGAGGC AAGCAAAGAT GTCAGTGTGA TTGATTTGCT GTACGGCTGG
    CCATTGTATC AGTTCCATTG CTGGTATGAG GTAACCTGTG TCCAGTGTTC CCCAGATGGA
    ATGTATGCTT TCTGTGGACA GTACCTCAAC ACAACCACAA TATTCCACCT TGGTAGTGGG
    GATAAGCTTG CCACAATGAC TTCTGAATTC TCAGGTGGTT TCGTGAAGTC CATTCTGATT
    CTAGACACAA TCAATCAAAT GGTGATGATT GACAATGAGG GCAGTCTCTC CGTATGGAGC
    ACCAAAGATA TCACAAACCC CAAGCTAATG GAGGATTATG ACTGCAGAGG AGATGATAGT
    GAGGTGGTGG GCATAGAGCT CTCAGAGGAT CAGCGATCCA TTCTAATCTG CAAAGCAAGA
    AGCATTGAGG TTCTGGACAC CAAAATTTGG AAGATGGCTG AAAAGTTTAA GGCCAAGCGC
    ACTGAGAAGT TTGTGGCAGC TGTTCTCTCC AAGAATAGCC AAAGTATAAT AGCCTCCATG
    GAGAACACAT CATCCATATT TGTGTGGAGG AGAGACAGTG GACAGTGCAT GGCCAGCTTG
    ATAGAGATAT CGGGAGCCAT TGTAAAGCTA ATCAAATCAA CACACCATAA CTTGCTTCTG
    TCTGTGGCCA GCAGTGGGGT GCTTTCCGTG TGGGATATCG ACATTATAAC AGCCATGTCG
    AACATTGATA AAACTGGAAA ACCTATCCAA ATTCTGCAAC TCTCTGGCAG AGAGGATTTT
    GTATACACCA TGGATGGGTC AGAAGTCATC CATAAATGGA ACTACAGCAC AGGTTTCATT
    GAAATGGTGT TCAAACATGA AGGACTTGTT GAGAACTGTG TCCTGACAAC ATCAGGTGAC
    CTAATGGTGA CATCTGACGA TAAAAGTAGT CAGTACATCT GGCATACGAC CACAGGTGAG
    AATATATTCC GTATCAATGG CCAGAAAATC TCACAGCTTC TCATCACGCA CAATGACCAA
    TTTGTTGTGT CACTTTGTGA GCAGAATGCC TCTCGTGTCT GGAGGCTTGC CACTGGTCAC
    AAGGTGTGCA ACATTCTGGT AACACTTCAA CATGCCCTCA TTACTACAGC AAACACATTC
    CTTGTGGGTG CAAACAAGAA TAAGCTGCTG GCTGTTAGCT TATGGTCAGG GAGTGTTTCC
    AAAAAGTTTA TCTGTGATGA TGGGATCACC ATTGTTAACT TCAAGTTAAT CCCTGACAGT
    CCAGATTTTG TTGTCTTCAT CACCTCCACT GAAACGGTAT TCATCTGGAG TGTTGCCGAT
    GAGTCAGTAT GTAGACGGGT GCAACTCCCC AACAACTTCT TAAAGAACCT GGAAGATTTC
    CAAATTTCAC CGAATGGGAA ATTGGGCATT GTCTCAAAAG GTGATGAGAA CATCAATGTT
    CTAGACCTAC ACAGTGGTAA GCTGCGTCTG GTGCATGCTG CTGGCATAAT ATGGCGGCAA
    AAGCTTTCAA GAGATGGACG CTACTTAGTA TACATCTGTT TCCGGAACTG CGATGAAGAT
    GATGATGCAG GTGTGGTTTC AAGCCTGATT GTCATGAGAC TGGCAGATGG TAAGAGTATC
    GGGACCTGTT CCCTTTACAA AACGCCAACC TATCTGTGCC TATCACAGCG AGCACTTAAC
    ATCATTGTCG GATTTGAGGA TGGCAGCATT GGCACTTATA CAGTGGTTGA TCGTGTAGAT
    GCTGCTCTGA AGATCAAGAT CGCCACCTCA AACAGTCGCC AAATTGTTAA CAACGCCTCA
    CAGAAAGTAC GACCCAAGTG TAGCAACAAT GCCTTCAAGA CCATTGCTGA CTGTGTGTGG
    AGAGAATCCA CTGAGGTCTT CTCTCGAGAC AGTCCTATCA ACGTGTCTGA TGGTGGGGAG
    CCAGAGACCA CCACACCCAC AAAGAAAACT GAACTGCTGC AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005172417.1
    CDS145..4929
    Translation

    Target ORF information:

    RefSeq Version XM_005172360.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio NACHT and WD repeat domain containing 2 (nwd2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005172360.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATTTTGG ATGCGGCAGT GGAGGCGGGA CTAGACACAC GGATCTTGGA GGAGTGGTAC 
    TGTCGAGATG AGAACTCAGT CCCACCTGCC TACTACCTAA AACCAAAAGC TGAGATGCTG
    AAAAACTATC AGAATTCTAT GGAGTCAAAC ACCGTAGCAA AATCAAAGAA CGACAAGGCA
    TGGAGGGCAG TCTCAGAGGA AATCAAGAGG ATCTTCAGAA CCTCTGTTTT ACAGCTGCAG
    GAGAAGGGGA CCATGAAGAG TGGTCAAGCC AAGAAATTTC TCTGCTCAGC ATTAGAGGAT
    GAACTGGACT TCGCATTGGG CAAGCAGACC CCAGCCTTTC TCAAAAAATG TGTTTGTTAC
    ATTCGGAAGA TATCTAACTT TGATCGTTTC GCTAAACTCC CCGAAATGGC ACGTTACATG
    GACATTGTGG TGAGCGATGA CCGTGTCATG AGAAACCAGG AAGCCTACGA GCGTCTGCTA
    AAGGTCAGAG ACGAGTTCAT TCCGGTGGTG GTGGCAGCAT CCAATCTCCG AGTCTACTCA
    TCGGTCACTC ACTGTGACAT GAAACTTGGC TACTCGCAGG AGGTAGAGAG CCATTACGTT
    GAAGGTCTGT GTAAACAGTT TTATGAAGAC ATGGTGGACA TCATCCAGGC GACGGTTCAG
    CAGAACTTTG ACACAGAGAC AGATCCTCTC TATGATGAAA TTATACAACA TTTGTCGCTG
    TGCAAGACGT TTTCATCTTT TTATGTGTAC AAGAGCGAAG CCTTGGATAT AGTCCAGGAG
    TACCTGTACC CCTCGAAAGG TGGTCGAATA ACTCCCCAAG TGGTTTACGG AGGACCCTGC
    ACTGGAAAGA CCTTGTTACT TGCTGAAGTG GCAAAACAGG CCTATTCATG GATACAGAAA
    GAGATGGGAC CAGAAACAGA CCCAGTGGTC ATTGTCAGAT TCATCGGTTC CACTGACTTC
    TCTACTGACC TCCGCACCCT GCTGCAGAGC ATCTGTGAGC AGATTGCCAT CAACTATCGG
    TGCCTGATCC ATTTCTTACC AAACAAGATC CAGGAGATGA GAGAACTGCT GGTGAATTTG
    CTGGGGGAGT CGTCATTCCA CCGACCTCTT GTCATTATTC TAGATGCCTT GGAGCAGTTG
    TCAGACGCAG ACGAGGCTCG AAAACTGTGG TGGCTCCCGG TCCACCTGCC ACGTACAGTT
    CGAATAGTAG TGTCCACCCT GCCCAACAAG CACGGAATAC TGCAGAAGCT GCGTTGCCTG
    ATACATGATG AGGAAGCCTA TGTTGAACTA ATGCAAAGAG ATCGCAAAGC TTGCAGTCAG
    ACCCTTAAAC AGCAGCTTCT GGGTGTCAAA AGGAAGGTGA CGTCTGGCCA ACAGATATAT
    GTTAATGAGG CAATGGCAAA ATGTACACTT CCTATGTTTG TTAACCTCAT CTACAGAGAG
    GTTGTTCACT GGAGATCGCA CAAGGATGTG GATGACAAAT CGTTGTGTTC CACAGTACAC
    GAAAGTATTG AGCAGCTTTT CTACTCAGTT GAAAATAAGC TTGGCTCACG CTTTGTCTTT
    CGGGCACTGG GCTACATCAC AATGGCACGG GCAGGCCTGA CTGAGCTGGA GCTAGAGGAT
    ATCTTATCTT TAGATAACAG TGTCCTAGGG GACATCATGG TGAGCTCTAA TCTCAAAAGC
    CCACTGCGTA TTTCCTATGA TTTCATAGCT CGGCTAAAAG AAGAACTTGA AGGTTATTTA
    GTGGAGCGTC AGGTCCATAA TGTAACACTA ATGGTGTGGG CAAATCGGCA TCTACATCTC
    ATAGCACAGA AGCTGTACCT CAGTAACGAA GAAGACGTGC ATCAAATGCA TAGCCTTTTG
    GCTGAGTACT TTCTTGGGGC ATGGGCAGGT GGCAGAAAGA AAATATTCCA CTGCGACAAT
    AATCACTTTG CCTCATTGAA TATCTCTCAG CATCGAAATC CTCACAACCA ACAGAGCCTT
    CATGGCCATG ACAAAGCTTC TGCTGACAAG CACTCATATG ACAGACAGAC ACCTGAGCAA
    CCCTGGGTGT TCCAATGTAA TCTGCTGGAG CCGGACATCT TCTTTGTCAA CCACCGTAAG
    ATGACAGAGC TCACATACCA CTTGACAAGG AGCGGCCGCA CAGATGATCT CATGTATGGT
    GTCATCATGA ACTTCAGTTG GCTCTACACT ATGATAAAGA TAGGGCAATT TGACAAGGCC
    CTGTCTGACA TTGACCTAGC CTACAGCTAT ACGCAAGAAA AGGAGCTAAA GTTTCTTGCA
    ACTACACTCC GCAGTATAAA GCTTAAAGTG ACAAAGAATC CAGCATCACT GTCTGCTGAA
    CTACAGCAAA GGCTCCTTCC AGTGGTCACA TCCCTTCCTA AACTCAGACA CCTCCTGCTT
    GAGTGTGACA AGGACGGCCC TAAATACTGC TCCATCGTGC CACTTCACTC CTCTATGGAT
    GTGACCTACA GTCCAGAGAG ATTGCCGCTC AGTTCTAGCT ACATGCAGAT AGTTGAGACT
    CTGCCTACAT TAGCTCCAAA CATAGTCATT GTGGCTCTTG AAGATGGGTC CGTCAGCACT
    TGGGATGTTG AAAGCAGACA GCTGCTAAGA CAGATAGACA CAGCAAGGTC AGTTGTTCTT
    GGAATTAGAC TAACCACTGA TGAGAAATAT CTGGTAGTGG CCACTACAAA AAATACACTG
    CTCATTTATG ACAACCACAA GTCTTGTTTG TTGTCTGAGG TAGAGGTCAA AGGGTCCAAA
    CACTGTGGGA TCACTGGTGG GGTGGCATTC ATTAATGGAT TTACCTTGTC CAGCCACCAT
    GCCTTAGCTT GGCTTGAGGC AAGCAAAGAT GTCAGTGTGA TTGATTTGCT GTACGGCTGG
    CCATTGTATC AGTTCCATTG CTGGTATGAG GTAACCTGTG TCCAGTGTTC CCCAGATGGA
    ATGTATGCTT TCTGTGGACA GTACCTCAAC ACAACCACAA TATTCCACCT TGGTAGTGGG
    GATAAGCTTG CCACAATGAC TTCTGAATTC TCAGGTGGTT TCGTGAAGTC CATTCTGATT
    CTAGACACAA TCAATCAAAT GGTGATGATT GACAATGAGG GCAGTCTCTC CGTATGGAGC
    ACCAAAGATA TCACAAACCC CAAGCTAATG GAGGATTATG ACTGCAGAGG AGATGATAGT
    GAGGTGGTGG GCATAGAGCT CTCAGAGGAT CAGCGATCCA TTCTAATCTG CAAAGCAAGA
    AGCATTGAGG TTCTGGACAC CAAAATTTGG AAGATGGCTG AAAAGTTTAA GGCCAAGCGC
    ACTGAGAAGT TTGTGGCAGC TGTTCTCTCC AAGAATAGCC AAAGTATAAT AGCCTCCATG
    GAGAACACAT CATCCATATT TGTGTGGAGG AGAGACAGTG GACAGTGCAT GGCCAGCTTG
    ATAGAGATAT CGGGAGCCAT TGTAAAGCTA ATCAAATCAA CACACCATAA CTTGCTTCTG
    TCTGTGGCCA GCAGTGGGGT GCTTTCCGTG TGGGATATCG ACATTATAAC AGCCATGTCG
    AACATTGATA AAACTGGAAA ACCTATCCAA ATTCTGCAAC TCTCTGGCAG AGAGGATTTT
    GTATACACCA TGGATGGGTC AGAAGTCATC CATAAATGGA ACTACAGCAC AGGTTTCATT
    GAAATGGTGT TCAAACATGA AGGACTTGTT GAGAACTGTG TCCTGACAAC ATCAGGTGAC
    CTAATGGTGA CATCTGACGA TAAAAGTAGT CAGTACATCT GGCATACGAC CACAGGTGAG
    AATATATTCC GTATCAATGG CCAGAAAATC TCACAGCTTC TCATCACGCA CAATGACCAA
    TTTGTTGTGT CACTTTGTGA GCAGAATGCC TCTCGTGTCT GGAGGCTTGC CACTGGTCAC
    AAGGTGTGCA ACATTCTGGT AACACTTCAA CATGCCCTCA TTACTACAGC AAACACATTC
    CTTGTGGGTG CAAACAAGAA TAAGCTGCTG GCTGTTAGCT TATGGTCAGG GAGTGTTTCC
    AAAAAGTTTA TCTGTGATGA TGGGATCACC ATTGTTAACT TCAAGTTAAT CCCTGACAGT
    CCAGATTTTG TTGTCTTCAT CACCTCCACT GAAACGGTAT TCATCTGGAG TGTTGCCGAT
    GAGTCAGTAT GTAGACGGGT GCAACTCCCC AACAACTTCT TAAAGAACCT GGAAGATTTC
    CAAATTTCAC CGAATGGGAA ATTGGGCATT GTCTCAAAAG GTGATGAGAA CATCAATGTT
    CTAGACCTAC ACAGTGGTAA GCTGCGTCTG GTGCATGCTG CTGGCATAAT ATGGCGGCAA
    AAGCTTTCAA GAGATGGACG CTACTTAGTA TACATCTGTT TCCGGAACTG CGATGAAGAT
    GATGATGCAG GTGTGGTTTC AAGCCTGATT GTCATGAGAC TGGCAGATGG TAAGAGTATC
    GGGACCTGTT CCCTTTACAA AACGCCAACC TATCTGTGCC TATCACAGCG AGCACTTAAC
    ATCATTGTCG GATTTGAGGA TGGCAGCATT GGCACTTATA CAGTGGTTGA TCGTGTAGAT
    GCTGCTCTGA AGATCAAGAT CGCCACCTCA AACAGTCGCC AAATTGTTAA CAACGCCTCA
    CAGAAAGTAC GACCCAAGTG TAGCAACAAT GCCTTCAAGA CCATTGCTGA CTGTGTGTGG
    AGAGAATCCA CTGAGGTCTT CTCTCGAGAC AGTCCTATCA ACGTGTCTGA TGGTGGGGAG
    CCAGAGACCA CCACACCCAC AAAGAAAACT GAACTGCTGC AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa13556
    Clone ID Related Accession (Same CDS sequence) XM_005172360.2 , XM_005172360.3
    Accession Version XM_005172360.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4785bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product NACHT and WD repeat domain-containing protein 2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172360.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATTTTGG ATGCGGCAGT GGAGGCGGGA CTAGACACAC GGATCTTGGA GGAGTGGTAC 
    TGTCGAGATG AGAACTCAGT CCCACCTGCC TACTACCTAA AACCAAAAGC TGAGATGCTG
    AAAAACTATC AGAATTCTAT GGAGTCAAAC ACCGTAGCAA AATCAAAGAA CGACAAGGCA
    TGGAGGGCAG TCTCAGAGGA AATCAAGAGG ATCTTCAGAA CCTCTGTTTT ACAGCTGCAG
    GAGAAGGGGA CCATGAAGAG TGGTCAAGCC AAGAAATTTC TCTGCTCAGC ATTAGAGGAT
    GAACTGGACT TCGCATTGGG CAAGCAGACC CCAGCCTTTC TCAAAAAATG TGTTTGTTAC
    ATTCGGAAGA TATCTAACTT TGATCGTTTC GCTAAACTCC CCGAAATGGC ACGTTACATG
    GACATTGTGG TGAGCGATGA CCGTGTCATG AGAAACCAGG AAGCCTACGA GCGTCTGCTA
    AAGGTCAGAG ACGAGTTCAT TCCGGTGGTG GTGGCAGCAT CCAATCTCCG AGTCTACTCA
    TCGGTCACTC ACTGTGACAT GAAACTTGGC TACTCGCAGG AGGTAGAGAG CCATTACGTT
    GAAGGTCTGT GTAAACAGTT TTATGAAGAC ATGGTGGACA TCATCCAGGC GACGGTTCAG
    CAGAACTTTG ACACAGAGAC AGATCCTCTC TATGATGAAA TTATACAACA TTTGTCGCTG
    TGCAAGACGT TTTCATCTTT TTATGTGTAC AAGAGCGAAG CCTTGGATAT AGTCCAGGAG
    TACCTGTACC CCTCGAAAGG TGGTCGAATA ACTCCCCAAG TGGTTTACGG AGGACCCTGC
    ACTGGAAAGA CCTTGTTACT TGCTGAAGTG GCAAAACAGG CCTATTCATG GATACAGAAA
    GAGATGGGAC CAGAAACAGA CCCAGTGGTC ATTGTCAGAT TCATCGGTTC CACTGACTTC
    TCTACTGACC TCCGCACCCT GCTGCAGAGC ATCTGTGAGC AGATTGCCAT CAACTATCGG
    TGCCTGATCC ATTTCTTACC AAACAAGATC CAGGAGATGA GAGAACTGCT GGTGAATTTG
    CTGGGGGAGT CGTCATTCCA CCGACCTCTT GTCATTATTC TAGATGCCTT GGAGCAGTTG
    TCAGACGCAG ACGAGGCTCG AAAACTGTGG TGGCTCCCGG TCCACCTGCC ACGTACAGTT
    CGAATAGTAG TGTCCACCCT GCCCAACAAG CACGGAATAC TGCAGAAGCT GCGTTGCCTG
    ATACATGATG AGGAAGCCTA TGTTGAACTA ATGCAAAGAG ATCGCAAAGC TTGCAGTCAG
    ACCCTTAAAC AGCAGCTTCT GGGTGTCAAA AGGAAGGTGA CGTCTGGCCA ACAGATATAT
    GTTAATGAGG CAATGGCAAA ATGTACACTT CCTATGTTTG TTAACCTCAT CTACAGAGAG
    GTTGTTCACT GGAGATCGCA CAAGGATGTG GATGACAAAT CGTTGTGTTC CACAGTACAC
    GAAAGTATTG AGCAGCTTTT CTACTCAGTT GAAAATAAGC TTGGCTCACG CTTTGTCTTT
    CGGGCACTGG GCTACATCAC AATGGCACGG GCAGGCCTGA CTGAGCTGGA GCTAGAGGAT
    ATCTTATCTT TAGATAACAG TGTCCTAGGG GACATCATGG TGAGCTCTAA TCTCAAAAGC
    CCACTGCGTA TTTCCTATGA TTTCATAGCT CGGCTAAAAG AAGAACTTGA AGGTTATTTA
    GTGGAGCGTC AGGTCCATAA TGTAACACTA ATGGTGTGGG CAAATCGGCA TCTACATCTC
    ATAGCACAGA AGCTGTACCT CAGTAACGAA GAAGACGTGC ATCAAATGCA TAGCCTTTTG
    GCTGAGTACT TTCTTGGGGC ATGGGCAGGT GGCAGAAAGA AAATATTCCA CTGCGACAAT
    AATCACTTTG CCTCATTGAA TATCTCTCAG CATCGAAATC CTCACAACCA ACAGAGCCTT
    CATGGCCATG ACAAAGCTTC TGCTGACAAG CACTCATATG ACAGACAGAC ACCTGAGCAA
    CCCTGGGTGT TCCAATGTAA TCTGCTGGAG CCGGACATCT TCTTTGTCAA CCACCGTAAG
    ATGACAGAGC TCACATACCA CTTGACAAGG AGCGGCCGCA CAGATGATCT CATGTATGGT
    GTCATCATGA ACTTCAGTTG GCTCTACACT ATGATAAAGA TAGGGCAATT TGACAAGGCC
    CTGTCTGACA TTGACCTAGC CTACAGCTAT ACGCAAGAAA AGGAGCTAAA GTTTCTTGCA
    ACTACACTCC GCAGTATAAA GCTTAAAGTG ACAAAGAATC CAGCATCACT GTCTGCTGAA
    CTACAGCAAA GGCTCCTTCC AGTGGTCACA TCCCTTCCTA AACTCAGACA CCTCCTGCTT
    GAGTGTGACA AGGACGGCCC TAAATACTGC TCCATCGTGC CACTTCACTC CTCTATGGAT
    GTGACCTACA GTCCAGAGAG ATTGCCGCTC AGTTCTAGCT ACATGCAGAT AGTTGAGACT
    CTGCCTACAT TAGCTCCAAA CATAGTCATT GTGGCTCTTG AAGATGGGTC CGTCAGCACT
    TGGGATGTTG AAAGCAGACA GCTGCTAAGA CAGATAGACA CAGCAAGGTC AGTTGTTCTT
    GGAATTAGAC TAACCACTGA TGAGAAATAT CTGGTAGTGG CCACTACAAA AAATACACTG
    CTCATTTATG ACAACCACAA GTCTTGTTTG TTGTCTGAGG TAGAGGTCAA AGGGTCCAAA
    CACTGTGGGA TCACTGGTGG GGTGGCATTC ATTAATGGAT TTACCTTGTC CAGCCACCAT
    GCCTTAGCTT GGCTTGAGGC AAGCAAAGAT GTCAGTGTGA TTGATTTGCT GTACGGCTGG
    CCATTGTATC AGTTCCATTG CTGGTATGAG GTAACCTGTG TCCAGTGTTC CCCAGATGGA
    ATGTATGCTT TCTGTGGACA GTACCTCAAC ACAACCACAA TATTCCACCT TGGTAGTGGG
    GATAAGCTTG CCACAATGAC TTCTGAATTC TCAGGTGGTT TCGTGAAGTC CATTCTGATT
    CTAGACACAA TCAATCAAAT GGTGATGATT GACAATGAGG GCAGTCTCTC CGTATGGAGC
    ACCAAAGATA TCACAAACCC CAAGCTAATG GAGGATTATG ACTGCAGAGG AGATGATAGT
    GAGGTGGTGG GCATAGAGCT CTCAGAGGAT CAGCGATCCA TTCTAATCTG CAAAGCAAGA
    AGCATTGAGG TTCTGGACAC CAAAATTTGG AAGATGGCTG AAAAGTTTAA GGCCAAGCGC
    ACTGAGAAGT TTGTGGCAGC TGTTCTCTCC AAGAATAGCC AAAGTATAAT AGCCTCCATG
    GAGAACACAT CATCCATATT TGTGTGGAGG AGAGACAGTG GACAGTGCAT GGCCAGCTTG
    ATAGAGATAT CGGGAGCCAT TGTAAAGCTA ATCAAATCAA CACACCATAA CTTGCTTCTG
    TCTGTGGCCA GCAGTGGGGT GCTTTCCGTG TGGGATATCG ACATTATAAC AGCCATGTCG
    AACATTGATA AAACTGGAAA ACCTATCCAA ATTCTGCAAC TCTCTGGCAG AGAGGATTTT
    GTATACACCA TGGATGGGTC AGAAGTCATC CATAAATGGA ACTACAGCAC AGGTTTCATT
    GAAATGGTGT TCAAACATGA AGGACTTGTT GAGAACTGTG TCCTGACAAC ATCAGGTGAC
    CTAATGGTGA CATCTGACGA TAAAAGTAGT CAGTACATCT GGCATACGAC CACAGGTGAG
    AATATATTCC GTATCAATGG CCAGAAAATC TCACAGCTTC TCATCACGCA CAATGACCAA
    TTTGTTGTGT CACTTTGTGA GCAGAATGCC TCTCGTGTCT GGAGGCTTGC CACTGGTCAC
    AAGGTGTGCA ACATTCTGGT AACACTTCAA CATGCCCTCA TTACTACAGC AAACACATTC
    CTTGTGGGTG CAAACAAGAA TAAGCTGCTG GCTGTTAGCT TATGGTCAGG GAGTGTTTCC
    AAAAAGTTTA TCTGTGATGA TGGGATCACC ATTGTTAACT TCAAGTTAAT CCCTGACAGT
    CCAGATTTTG TTGTCTTCAT CACCTCCACT GAAACGGTAT TCATCTGGAG TGTTGCCGAT
    GAGTCAGTAT GTAGACGGGT GCAACTCCCC AACAACTTCT TAAAGAACCT GGAAGATTTC
    CAAATTTCAC CGAATGGGAA ATTGGGCATT GTCTCAAAAG GTGATGAGAA CATCAATGTT
    CTAGACCTAC ACAGTGGTAA GCTGCGTCTG GTGCATGCTG CTGGCATAAT ATGGCGGCAA
    AAGCTTTCAA GAGATGGACG CTACTTAGTA TACATCTGTT TCCGGAACTG CGATGAAGAT
    GATGATGCAG GTGTGGTTTC AAGCCTGATT GTCATGAGAC TGGCAGATGG TAAGAGTATC
    GGGACCTGTT CCCTTTACAA AACGCCAACC TATCTGTGCC TATCACAGCG AGCACTTAAC
    ATCATTGTCG GATTTGAGGA TGGCAGCATT GGCACTTATA CAGTGGTTGA TCGTGTAGAT
    GCTGCTCTGA AGATCAAGAT CGCCACCTCA AACAGTCGCC AAATTGTTAA CAACGCCTCA
    CAGAAAGTAC GACCCAAGTG TAGCAACAAT GCCTTCAAGA CCATTGCTGA CTGTGTGTGG
    AGAGAATCCA CTGAGGTCTT CTCTCGAGAC AGTCCTATCA ACGTGTCTGA TGGTGGGGAG
    CCAGAGACCA CCACACCCAC AAAGAAAACT GAACTGCTGC AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005172417.1
    CDS194..4978
    Translation

    Target ORF information:

    RefSeq Version XM_005172360.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NACHT and WD repeat domain containing 2 (nwd2), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005172360.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    ATGATTTTGG ATGCGGCAGT GGAGGCGGGA CTAGACACAC GGATCTTGGA GGAGTGGTAC 
    TGTCGAGATG AGAACTCAGT CCCACCTGCC TACTACCTAA AACCAAAAGC TGAGATGCTG
    AAAAACTATC AGAATTCTAT GGAGTCAAAC ACCGTAGCAA AATCAAAGAA CGACAAGGCA
    TGGAGGGCAG TCTCAGAGGA AATCAAGAGG ATCTTCAGAA CCTCTGTTTT ACAGCTGCAG
    GAGAAGGGGA CCATGAAGAG TGGTCAAGCC AAGAAATTTC TCTGCTCAGC ATTAGAGGAT
    GAACTGGACT TCGCATTGGG CAAGCAGACC CCAGCCTTTC TCAAAAAATG TGTTTGTTAC
    ATTCGGAAGA TATCTAACTT TGATCGTTTC GCTAAACTCC CCGAAATGGC ACGTTACATG
    GACATTGTGG TGAGCGATGA CCGTGTCATG AGAAACCAGG AAGCCTACGA GCGTCTGCTA
    AAGGTCAGAG ACGAGTTCAT TCCGGTGGTG GTGGCAGCAT CCAATCTCCG AGTCTACTCA
    TCGGTCACTC ACTGTGACAT GAAACTTGGC TACTCGCAGG AGGTAGAGAG CCATTACGTT
    GAAGGTCTGT GTAAACAGTT TTATGAAGAC ATGGTGGACA TCATCCAGGC GACGGTTCAG
    CAGAACTTTG ACACAGAGAC AGATCCTCTC TATGATGAAA TTATACAACA TTTGTCGCTG
    TGCAAGACGT TTTCATCTTT TTATGTGTAC AAGAGCGAAG CCTTGGATAT AGTCCAGGAG
    TACCTGTACC CCTCGAAAGG TGGTCGAATA ACTCCCCAAG TGGTTTACGG AGGACCCTGC
    ACTGGAAAGA CCTTGTTACT TGCTGAAGTG GCAAAACAGG CCTATTCATG GATACAGAAA
    GAGATGGGAC CAGAAACAGA CCCAGTGGTC ATTGTCAGAT TCATCGGTTC CACTGACTTC
    TCTACTGACC TCCGCACCCT GCTGCAGAGC ATCTGTGAGC AGATTGCCAT CAACTATCGG
    TGCCTGATCC ATTTCTTACC AAACAAGATC CAGGAGATGA GAGAACTGCT GGTGAATTTG
    CTGGGGGAGT CGTCATTCCA CCGACCTCTT GTCATTATTC TAGATGCCTT GGAGCAGTTG
    TCAGACGCAG ACGAGGCTCG AAAACTGTGG TGGCTCCCGG TCCACCTGCC ACGTACAGTT
    CGAATAGTAG TGTCCACCCT GCCCAACAAG CACGGAATAC TGCAGAAGCT GCGTTGCCTG
    ATACATGATG AGGAAGCCTA TGTTGAACTA ATGCAAAGAG ATCGCAAAGC TTGCAGTCAG
    ACCCTTAAAC AGCAGCTTCT GGGTGTCAAA AGGAAGGTGA CGTCTGGCCA ACAGATATAT
    GTTAATGAGG CAATGGCAAA ATGTACACTT CCTATGTTTG TTAACCTCAT CTACAGAGAG
    GTTGTTCACT GGAGATCGCA CAAGGATGTG GATGACAAAT CGTTGTGTTC CACAGTACAC
    GAAAGTATTG AGCAGCTTTT CTACTCAGTT GAAAATAAGC TTGGCTCACG CTTTGTCTTT
    CGGGCACTGG GCTACATCAC AATGGCACGG GCAGGCCTGA CTGAGCTGGA GCTAGAGGAT
    ATCTTATCTT TAGATAACAG TGTCCTAGGG GACATCATGG TGAGCTCTAA TCTCAAAAGC
    CCACTGCGTA TTTCCTATGA TTTCATAGCT CGGCTAAAAG AAGAACTTGA AGGTTATTTA
    GTGGAGCGTC AGGTCCATAA TGTAACACTA ATGGTGTGGG CAAATCGGCA TCTACATCTC
    ATAGCACAGA AGCTGTACCT CAGTAACGAA GAAGACGTGC ATCAAATGCA TAGCCTTTTG
    GCTGAGTACT TTCTTGGGGC ATGGGCAGGT GGCAGAAAGA AAATATTCCA CTGCGACAAT
    AATCACTTTG CCTCATTGAA TATCTCTCAG CATCGAAATC CTCACAACCA ACAGAGCCTT
    CATGGCCATG ACAAAGCTTC TGCTGACAAG CACTCATATG ACAGACAGAC ACCTGAGCAA
    CCCTGGGTGT TCCAATGTAA TCTGCTGGAG CCGGACATCT TCTTTGTCAA CCACCGTAAG
    ATGACAGAGC TCACATACCA CTTGACAAGG AGCGGCCGCA CAGATGATCT CATGTATGGT
    GTCATCATGA ACTTCAGTTG GCTCTACACT ATGATAAAGA TAGGGCAATT TGACAAGGCC
    CTGTCTGACA TTGACCTAGC CTACAGCTAT ACGCAAGAAA AGGAGCTAAA GTTTCTTGCA
    ACTACACTCC GCAGTATAAA GCTTAAAGTG ACAAAGAATC CAGCATCACT GTCTGCTGAA
    CTACAGCAAA GGCTCCTTCC AGTGGTCACA TCCCTTCCTA AACTCAGACA CCTCCTGCTT
    GAGTGTGACA AGGACGGCCC TAAATACTGC TCCATCGTGC CACTTCACTC CTCTATGGAT
    GTGACCTACA GTCCAGAGAG ATTGCCGCTC AGTTCTAGCT ACATGCAGAT AGTTGAGACT
    CTGCCTACAT TAGCTCCAAA CATAGTCATT GTGGCTCTTG AAGATGGGTC CGTCAGCACT
    TGGGATGTTG AAAGCAGACA GCTGCTAAGA CAGATAGACA CAGCAAGGTC AGTTGTTCTT
    GGAATTAGAC TAACCACTGA TGAGAAATAT CTGGTAGTGG CCACTACAAA AAATACACTG
    CTCATTTATG ACAACCACAA GTCTTGTTTG TTGTCTGAGG TAGAGGTCAA AGGGTCCAAA
    CACTGTGGGA TCACTGGTGG GGTGGCATTC ATTAATGGAT TTACCTTGTC CAGCCACCAT
    GCCTTAGCTT GGCTTGAGGC AAGCAAAGAT GTCAGTGTGA TTGATTTGCT GTACGGCTGG
    CCATTGTATC AGTTCCATTG CTGGTATGAG GTAACCTGTG TCCAGTGTTC CCCAGATGGA
    ATGTATGCTT TCTGTGGACA GTACCTCAAC ACAACCACAA TATTCCACCT TGGTAGTGGG
    GATAAGCTTG CCACAATGAC TTCTGAATTC TCAGGTGGTT TCGTGAAGTC CATTCTGATT
    CTAGACACAA TCAATCAAAT GGTGATGATT GACAATGAGG GCAGTCTCTC CGTATGGAGC
    ACCAAAGATA TCACAAACCC CAAGCTAATG GAGGATTATG ACTGCAGAGG AGATGATAGT
    GAGGTGGTGG GCATAGAGCT CTCAGAGGAT CAGCGATCCA TTCTAATCTG CAAAGCAAGA
    AGCATTGAGG TTCTGGACAC CAAAATTTGG AAGATGGCTG AAAAGTTTAA GGCCAAGCGC
    ACTGAGAAGT TTGTGGCAGC TGTTCTCTCC AAGAATAGCC AAAGTATAAT AGCCTCCATG
    GAGAACACAT CATCCATATT TGTGTGGAGG AGAGACAGTG GACAGTGCAT GGCCAGCTTG
    ATAGAGATAT CGGGAGCCAT TGTAAAGCTA ATCAAATCAA CACACCATAA CTTGCTTCTG
    TCTGTGGCCA GCAGTGGGGT GCTTTCCGTG TGGGATATCG ACATTATAAC AGCCATGTCG
    AACATTGATA AAACTGGAAA ACCTATCCAA ATTCTGCAAC TCTCTGGCAG AGAGGATTTT
    GTATACACCA TGGATGGGTC AGAAGTCATC CATAAATGGA ACTACAGCAC AGGTTTCATT
    GAAATGGTGT TCAAACATGA AGGACTTGTT GAGAACTGTG TCCTGACAAC ATCAGGTGAC
    CTAATGGTGA CATCTGACGA TAAAAGTAGT CAGTACATCT GGCATACGAC CACAGGTGAG
    AATATATTCC GTATCAATGG CCAGAAAATC TCACAGCTTC TCATCACGCA CAATGACCAA
    TTTGTTGTGT CACTTTGTGA GCAGAATGCC TCTCGTGTCT GGAGGCTTGC CACTGGTCAC
    AAGGTGTGCA ACATTCTGGT AACACTTCAA CATGCCCTCA TTACTACAGC AAACACATTC
    CTTGTGGGTG CAAACAAGAA TAAGCTGCTG GCTGTTAGCT TATGGTCAGG GAGTGTTTCC
    AAAAAGTTTA TCTGTGATGA TGGGATCACC ATTGTTAACT TCAAGTTAAT CCCTGACAGT
    CCAGATTTTG TTGTCTTCAT CACCTCCACT GAAACGGTAT TCATCTGGAG TGTTGCCGAT
    GAGTCAGTAT GTAGACGGGT GCAACTCCCC AACAACTTCT TAAAGAACCT GGAAGATTTC
    CAAATTTCAC CGAATGGGAA ATTGGGCATT GTCTCAAAAG GTGATGAGAA CATCAATGTT
    CTAGACCTAC ACAGTGGTAA GCTGCGTCTG GTGCATGCTG CTGGCATAAT ATGGCGGCAA
    AAGCTTTCAA GAGATGGACG CTACTTAGTA TACATCTGTT TCCGGAACTG CGATGAAGAT
    GATGATGCAG GTGTGGTTTC AAGCCTGATT GTCATGAGAC TGGCAGATGG TAAGAGTATC
    GGGACCTGTT CCCTTTACAA AACGCCAACC TATCTGTGCC TATCACAGCG AGCACTTAAC
    ATCATTGTCG GATTTGAGGA TGGCAGCATT GGCACTTATA CAGTGGTTGA TCGTGTAGAT
    GCTGCTCTGA AGATCAAGAT CGCCACCTCA AACAGTCGCC AAATTGTTAA CAACGCCTCA
    CAGAAAGTAC GACCCAAGTG TAGCAACAAT GCCTTCAAGA CCATTGCTGA CTGTGTGTGG
    AGAGAATCCA CTGAGGTCTT CTCTCGAGAC AGTCCTATCA ACGTGTCTGA TGGTGGGGAG
    CCAGAGACCA CCACACCCAC AAAGAAAACT GAACTGCTGC AATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43788
    Clone ID Related Accession (Same CDS sequence) XM_009303553.2 , XM_009303553.3
    Accession Version XM_009303553.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product NACHT and WD repeat domain-containing protein 2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303553.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTGGCCAT CCGGAGCGGG CAGCAGGCAG CCCTGTCCCC GTGAATCTGC ACTGCGCAGA 
    GCAGCCATCA GTGGCAACGT CCTTGCCCTC CCACCATTCC ATGTCCCCAC GGGCAGGAGT
    GTCCGGGTCT TCATCTGTGC CAACCCTGAC GATACAGAAG CAGAGAGAAA TGCTCTGAAA
    GAACATGTCT ACCCCAAACT ACGAGACTTC TGCCGAGAGA ACTATGGCAT TGAATTCCAG
    GTGGTGGACC TGTACTGGGG CATTGATCCA GAGGAGTGGG ACAGTCCGGA GCTGCAGAGA
    CTGCGGATGA AACTACTGGA AGAATGTCTG AAGACTTCAG CTGGTCCATG CTTTGTTGGG
    CTCATAGGAG AGAAGTATGG CAGCATTCGG GTCCCAGGGG AGGTGGAGTC AGCTGAGTTT
    GAGATGATTT TGGATGCGGC AGTGGAGGCG GGACTAGACA CACGGATCTT GGAGGAGTGG
    TACTGTCGAG ATGAGAACTC AGTCCCACCT GCCTACTACC TAAAACCAAA AGCTGAGATG
    CTGAAAAACT ATCAGAATTC TATGGAGTCA AACACCGTAG CAAAATCAAA GAACGACAAG
    GCATGGAGGG CAGTCTCAGA GGAAATCAAG AGGATCTTCA GAACCTCTGT TTTACAGCTG
    CAGGAGAAGG GGACCATGAA GAGTGGTCAA GCCAAGAAAT TTCTCTGCTC AGCATTAGAG
    GATGAACTGG ACTTCGCATT GGGCAAGCAG ACCCCAGCCT TTCTCAAAAA ATGTGTTTGT
    TACATTCGGA AGATATCTAA CTTTGATCGT TTCGCTAAAC TCCCCGAAAT GGCACGTTAC
    ATGGACATTG TGGTGAGCGA TGACCGTGTC ATGAGAAACC AGGAAGCCTA CGAGCGTCTG
    CTAAAGGTCA GAGACGAGTT CATTCCGGTG GTGGTGGCAG CATCCAATCT CCGAGTCTAC
    TCATCGGTCA CTCACTGTGA CATGAAACTT GGCTACTCGC AGGAGGTAGA GAGCCATTAC
    GTTGAAGGTC TGTGTAAACA GTTTTATGAA GACATGGTGG ACATCATCCA GGCGACGGTT
    CAGCAGAACT TTGACACAGA GACAGATCCT CTCTATGATG AAATTATACA ACATTTGTCG
    CTGTGCAAGA CGTTTTCATC TTTTTATGTG TACAAGAGCG AAGCCTTGGA TATAGTCCAG
    GAGTACCTGT ACCCCTCGAA AGGTGGTCGA ATAACTCCCC AAGTGGTTTA CGGAGGACCC
    TGCACTGGAA AGACCTTGTT ACTTGCTGAA GTGGCAAAAC AGGCCTATTC ATGGATACAG
    AAAGAGATGG GACCAGAAAC AGACCCAGTG GTCATTGTCA GATTCATCGG TTCCACTGAC
    TTCTCTACTG ACCTCCGCAC CCTGCTGCAG AGCATCTGTG AGCAGATTGC CATCAACTAT
    CGGTGCCTGA TCCATTTCTT ACCAAACAAG ATCCAGGAGA TGAGAGAACT GCTGGTGAAT
    TTGCTGGGGG AGTCGTCATT CCACCGACCT CTTGTCATTA TTCTAGATGC CTTGGAGCAG
    TTGTCAGACG CAGACGAGGC TCGAAAACTG TGGTGGCTCC CGGTCCACCT GCCACGTACA
    GTTCGAATAG TAGTGTCCAC CCTGCCCAAC AAGCACGGAA TACTGCAGAA GCTGCGTTGC
    CTGATACATG ATGAGGAAGC CTATGTTGAA CTAATGCAAA GAGATCGCAA AGCTTGCAGT
    CAGACCCTTA AACAGCAGCT TCTGGGTGTC AAAAGGAAGG TGACGTCTGG CCAACAGATA
    TATGTTAATG AGGCAATGGC AAAATGTACA CTTCCTATGT TTGTTAACCT CATCTACAGA
    GAGGTTGTTC ACTGGAGATC GCACAAGGAT GTGGATGACA AATCGTTGTG TTCCACAGTA
    CACGAAAGTA TTGAGCAGCT TTTCTACTCA GTTGAAAATA AGCTTGGCTC ACGCTTTGTC
    TTTCGGGCAC TGGGCTACAT CACAATGGCA CGGGCAGGCC TGACTGAGCT GGAGCTAGAG
    GATATCTTAT CTTTAGATAA CAGTGTCCTA GGGGACATCA TGGTGAGCTC TAATCTCAAA
    AGCCCACTGC GTATTTCCTA TGATTTCATA GCTCGGCTAA AAGAAGAACT TGAAGGTTAT
    TTAGTGGAGC GTCAGGTCCA TAATGTAACA CTAATGGTGT GGGCAAATCG GCATCTACAT
    CTCATAGCAC AGAAGCTGTA CCTCAGTAAC GAAGAAGACG TGCATCAAAT GCATAGCCTT
    TTGGCTGAGT ACTTTCTTGG GGCATGGGCA GGTGGCAGAA AGAAAATATT CCACTGCGAC
    AATAATCACT TTGCCTCATT GAATATCTCT CAGCATCGAA ATCCTCACAA CCAACAGAGC
    CTTCATGGCC ATGACAAAGC TTCTGCTGAC AAGCACTCAT ATGACAGACA GACACCTGAG
    CAACCCTGGG TGTTCCAATG TAATCTGCTG GAGCCGGACA TCTTCTTTGT CAACCACCGT
    AAGATGACAG AGCTCACATA CCACTTGACA AGGAGCGGCC GCACAGATGA TCTCATGTAT
    GGTGTCATCA TGAACTTCAG TTGGCTCTAC ACTATGATAA AGATAGGGCA ATTTGACAAG
    GCCCTGTCTG ACATTGACCT AGCCTACAGC TATACGCAAG AAAAGGAGCT AAAGTTTCTT
    GCAACTACAC TCCGCAGTAT AAAGCTTAAA GTGACAAAGA ATCCAGCATC ACTGTCTGCT
    GAACTACAGC AAAGGCTCCT TCCAGTGGTC ACATCCCTTC CTAAACTCAG ACACCTCCTG
    CTTGAGTGTG ACAAGGACGG CCCTAAATAC TGCTCCATCG TGCCACTTCA CTCCTCTATG
    GATGTGACCT ACAGTCCAGA GAGATTGCCG CTCAGTTCTA GCTACATGCA GATAGTTGAG
    ACTCTGCCTA CATTAGCTCC AAACATAGTC ATTGTGGCTC TTGAAGATGG GTCCGTCAGC
    ACTTGGGATG TTGAAAGCAG ACAGCTGCTA AGACAGATAG ACACAGCAAG GTCAGTTGTT
    CTTGGAATTA GACTAACCAC TGATGAGAAA TATCTGGTAG TGGCCACTAC AAAAAATACA
    CTGCTCATTT ATGACAACCA CAAGTCTTGT TTGTTGTCTG AGGTAGAGGT CAAAGGGTCC
    AAACACTGTG GGATCACTGG TGGGGTGGCA TTCATTAATG GATTTACCTT GTCCAGCCAC
    CATGCCTTAG CTTGGCTTGA GGCAAGCAAA GATGTCAGTG TGATTGATTT GCTGTACGGC
    TGGCCATTGT ATCAGTTCCA TTGCTGGTAT GAGGTAACCT GTGTCCAGTG TTCCCCAGAT
    GGAATGTATG CTTTCTGTGG ACAGTACCTC AACACAACCA CAATATTCCA CCTTGGTAGT
    GGGGATAAGC TTGCCACAAT GACTTCTGAA TTCTCAGGTG GTTTCGTGAA GTCCATTCTG
    ATTCTAGACA CAATCAATCA AATGGTGATG ATTGACAATG AGGGCAGTCT CTCCGTATGG
    AGCACCAAAG ATATCACAAA CCCCAAGCTA ATGGAGGATT ATGACTGCAG AGGAGATGAT
    AGTGAGGTGG TGGGCATAGA GCTCTCAGAG GATCAGCGAT CCATTCTAAT CTGCAAAGCA
    AGAAGCATTG AGGTTCTGGA CACCAAAATT TGGAAGATGG CTGAAAAGTT TAAGGCCAAG
    CGCACTGAGA AGTTTGTGGC AGCTGTTCTC TCCAAGAATA GCCAAAGTAT AATAGCCTCC
    ATGGAGAACA CATCATCCAT ATTTGTGTGG AGGAGAGACA GTGGACAGTG CATGGCCAGC
    TTGATAGAGA TATCGGGAGC CATTGTAAAG CTAATCAAAT CAACACACCA TAACTTGCTT
    CTGTCTGTGG CCAGCAGTGG GGTGCTTTCC GTGTGGGATA TCGACATTAT AACAGCCATG
    TCGAACATTG ATAAAACTGG AAAACCTATC CAAATTCTGC AACTCTCTGG CAGAGAGGAT
    TTTGTATACA CCATGGATGG GTCAGAAGTC ATCCATAAAT GGAACTACAG CACAGGTTTC
    ATTGAAATGG TGTTCAAACA TGAAGGACTT GTTGAGAACT GTGTCCTGAC AACATCAGGT
    GACCTAATGG TGACATCTGA CGATAAAAGT AGTCAGTACA TCTGGCATAC GACCACAGGT
    GAGAATATAT TCCGTATCAA TGGCCAGAAA ATCTCACAGC TTCTCATCAC GCACAATGAC
    CAATTTGTTG TGTCACTTTG TGAGCAGAAT GCCTCTCGTG TCTGGAGGCT TGCCACTGGT
    CACAAGGTGT GCAACATTCT GGTAACACTT CAACATGCCC TCATTACTAC AGCAAACACA
    TTCCTTGTGG GTGCAAACAA GAATAAGCTG CTGGCTGTTA GCTTATGGTC AGGGAGTGTT
    TCCAAAAAGT TTATCTGTGA TGATGGGATC ACCATTGTTA ACTTCAAGTT AATCCCTGAC
    AGTCCAGATT TTGTTGTCTT CATCACCTCC ACTGAAACGG TATTCATCTG GAGTGTTGCC
    GATGAGTCAG TATGTAGACG GGTGCAACTC CCCAACAACT TCTTAAAGAA CCTGGAAGAT
    TTCCAAATTT CACCGAATGG GAAATTGGGC ATTGTCTCAA AAGGTGATGA GAACATCAAT
    GTTCTAGACC TACACAGTGG TAAGCTGCGT CTGGTGCATG CTGCTGGCAT AATATGGCGG
    CAAAAGCTTT CAAGAGATGG ACGCTACTTA GTATACATCT GTTTCCGGAA CTGCGATGAA
    GATGATGATG CAGGTGTGGT TTCAAGCCTG ATTGTCATGA GACTGGCAGA TGGTAAGAGT
    ATCGGGACCT GTTCCCTTTA CAAAACGCCA ACCTATCTGT GCCTATCACA GCGAGCACTT
    AACATCATTG TCGGATTTGA GGATGGCAGC ATTGGCACTT ATACAGTGGT TGATCGTGTA
    GATGCTGCTC TGAAGATCAA GATCGCCACC TCAAACAGTC GCCAAATTGT TAACAACGCC
    TCACAGAAAG TACGACCCAA GTGTAGCAAC AATGCCTTCA AGACCATTGC TGACTGTGTG
    TGGAGAGAAT CCACTGAGGT CTTCTCTCGA GACAGTCCTA TCAACGTGTC TGATGGTGGG
    GAGCCAGAGA CCACCACACC CACAAAGAAA ACTGAACTGC TGCAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301828.1
    CDS141..5348
    Translation

    Target ORF information:

    RefSeq Version XM_009303553.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio NACHT and WD repeat domain containing 2 (nwd2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303553.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTGGCCAT CCGGAGCGGG CAGCAGGCAG CCCTGTCCCC GTGAATCTGC ACTGCGCAGA 
    GCAGCCATCA GTGGCAACGT CCTTGCCCTC CCACCATTCC ATGTCCCCAC GGGCAGGAGT
    GTCCGGGTCT TCATCTGTGC CAACCCTGAC GATACAGAAG CAGAGAGAAA TGCTCTGAAA
    GAACATGTCT ACCCCAAACT ACGAGACTTC TGCCGAGAGA ACTATGGCAT TGAATTCCAG
    GTGGTGGACC TGTACTGGGG CATTGATCCA GAGGAGTGGG ACAGTCCGGA GCTGCAGAGA
    CTGCGGATGA AACTACTGGA AGAATGTCTG AAGACTTCAG CTGGTCCATG CTTTGTTGGG
    CTCATAGGAG AGAAGTATGG CAGCATTCGG GTCCCAGGGG AGGTGGAGTC AGCTGAGTTT
    GAGATGATTT TGGATGCGGC AGTGGAGGCG GGACTAGACA CACGGATCTT GGAGGAGTGG
    TACTGTCGAG ATGAGAACTC AGTCCCACCT GCCTACTACC TAAAACCAAA AGCTGAGATG
    CTGAAAAACT ATCAGAATTC TATGGAGTCA AACACCGTAG CAAAATCAAA GAACGACAAG
    GCATGGAGGG CAGTCTCAGA GGAAATCAAG AGGATCTTCA GAACCTCTGT TTTACAGCTG
    CAGGAGAAGG GGACCATGAA GAGTGGTCAA GCCAAGAAAT TTCTCTGCTC AGCATTAGAG
    GATGAACTGG ACTTCGCATT GGGCAAGCAG ACCCCAGCCT TTCTCAAAAA ATGTGTTTGT
    TACATTCGGA AGATATCTAA CTTTGATCGT TTCGCTAAAC TCCCCGAAAT GGCACGTTAC
    ATGGACATTG TGGTGAGCGA TGACCGTGTC ATGAGAAACC AGGAAGCCTA CGAGCGTCTG
    CTAAAGGTCA GAGACGAGTT CATTCCGGTG GTGGTGGCAG CATCCAATCT CCGAGTCTAC
    TCATCGGTCA CTCACTGTGA CATGAAACTT GGCTACTCGC AGGAGGTAGA GAGCCATTAC
    GTTGAAGGTC TGTGTAAACA GTTTTATGAA GACATGGTGG ACATCATCCA GGCGACGGTT
    CAGCAGAACT TTGACACAGA GACAGATCCT CTCTATGATG AAATTATACA ACATTTGTCG
    CTGTGCAAGA CGTTTTCATC TTTTTATGTG TACAAGAGCG AAGCCTTGGA TATAGTCCAG
    GAGTACCTGT ACCCCTCGAA AGGTGGTCGA ATAACTCCCC AAGTGGTTTA CGGAGGACCC
    TGCACTGGAA AGACCTTGTT ACTTGCTGAA GTGGCAAAAC AGGCCTATTC ATGGATACAG
    AAAGAGATGG GACCAGAAAC AGACCCAGTG GTCATTGTCA GATTCATCGG TTCCACTGAC
    TTCTCTACTG ACCTCCGCAC CCTGCTGCAG AGCATCTGTG AGCAGATTGC CATCAACTAT
    CGGTGCCTGA TCCATTTCTT ACCAAACAAG ATCCAGGAGA TGAGAGAACT GCTGGTGAAT
    TTGCTGGGGG AGTCGTCATT CCACCGACCT CTTGTCATTA TTCTAGATGC CTTGGAGCAG
    TTGTCAGACG CAGACGAGGC TCGAAAACTG TGGTGGCTCC CGGTCCACCT GCCACGTACA
    GTTCGAATAG TAGTGTCCAC CCTGCCCAAC AAGCACGGAA TACTGCAGAA GCTGCGTTGC
    CTGATACATG ATGAGGAAGC CTATGTTGAA CTAATGCAAA GAGATCGCAA AGCTTGCAGT
    CAGACCCTTA AACAGCAGCT TCTGGGTGTC AAAAGGAAGG TGACGTCTGG CCAACAGATA
    TATGTTAATG AGGCAATGGC AAAATGTACA CTTCCTATGT TTGTTAACCT CATCTACAGA
    GAGGTTGTTC ACTGGAGATC GCACAAGGAT GTGGATGACA AATCGTTGTG TTCCACAGTA
    CACGAAAGTA TTGAGCAGCT TTTCTACTCA GTTGAAAATA AGCTTGGCTC ACGCTTTGTC
    TTTCGGGCAC TGGGCTACAT CACAATGGCA CGGGCAGGCC TGACTGAGCT GGAGCTAGAG
    GATATCTTAT CTTTAGATAA CAGTGTCCTA GGGGACATCA TGGTGAGCTC TAATCTCAAA
    AGCCCACTGC GTATTTCCTA TGATTTCATA GCTCGGCTAA AAGAAGAACT TGAAGGTTAT
    TTAGTGGAGC GTCAGGTCCA TAATGTAACA CTAATGGTGT GGGCAAATCG GCATCTACAT
    CTCATAGCAC AGAAGCTGTA CCTCAGTAAC GAAGAAGACG TGCATCAAAT GCATAGCCTT
    TTGGCTGAGT ACTTTCTTGG GGCATGGGCA GGTGGCAGAA AGAAAATATT CCACTGCGAC
    AATAATCACT TTGCCTCATT GAATATCTCT CAGCATCGAA ATCCTCACAA CCAACAGAGC
    CTTCATGGCC ATGACAAAGC TTCTGCTGAC AAGCACTCAT ATGACAGACA GACACCTGAG
    CAACCCTGGG TGTTCCAATG TAATCTGCTG GAGCCGGACA TCTTCTTTGT CAACCACCGT
    AAGATGACAG AGCTCACATA CCACTTGACA AGGAGCGGCC GCACAGATGA TCTCATGTAT
    GGTGTCATCA TGAACTTCAG TTGGCTCTAC ACTATGATAA AGATAGGGCA ATTTGACAAG
    GCCCTGTCTG ACATTGACCT AGCCTACAGC TATACGCAAG AAAAGGAGCT AAAGTTTCTT
    GCAACTACAC TCCGCAGTAT AAAGCTTAAA GTGACAAAGA ATCCAGCATC ACTGTCTGCT
    GAACTACAGC AAAGGCTCCT TCCAGTGGTC ACATCCCTTC CTAAACTCAG ACACCTCCTG
    CTTGAGTGTG ACAAGGACGG CCCTAAATAC TGCTCCATCG TGCCACTTCA CTCCTCTATG
    GATGTGACCT ACAGTCCAGA GAGATTGCCG CTCAGTTCTA GCTACATGCA GATAGTTGAG
    ACTCTGCCTA CATTAGCTCC AAACATAGTC ATTGTGGCTC TTGAAGATGG GTCCGTCAGC
    ACTTGGGATG TTGAAAGCAG ACAGCTGCTA AGACAGATAG ACACAGCAAG GTCAGTTGTT
    CTTGGAATTA GACTAACCAC TGATGAGAAA TATCTGGTAG TGGCCACTAC AAAAAATACA
    CTGCTCATTT ATGACAACCA CAAGTCTTGT TTGTTGTCTG AGGTAGAGGT CAAAGGGTCC
    AAACACTGTG GGATCACTGG TGGGGTGGCA TTCATTAATG GATTTACCTT GTCCAGCCAC
    CATGCCTTAG CTTGGCTTGA GGCAAGCAAA GATGTCAGTG TGATTGATTT GCTGTACGGC
    TGGCCATTGT ATCAGTTCCA TTGCTGGTAT GAGGTAACCT GTGTCCAGTG TTCCCCAGAT
    GGAATGTATG CTTTCTGTGG ACAGTACCTC AACACAACCA CAATATTCCA CCTTGGTAGT
    GGGGATAAGC TTGCCACAAT GACTTCTGAA TTCTCAGGTG GTTTCGTGAA GTCCATTCTG
    ATTCTAGACA CAATCAATCA AATGGTGATG ATTGACAATG AGGGCAGTCT CTCCGTATGG
    AGCACCAAAG ATATCACAAA CCCCAAGCTA ATGGAGGATT ATGACTGCAG AGGAGATGAT
    AGTGAGGTGG TGGGCATAGA GCTCTCAGAG GATCAGCGAT CCATTCTAAT CTGCAAAGCA
    AGAAGCATTG AGGTTCTGGA CACCAAAATT TGGAAGATGG CTGAAAAGTT TAAGGCCAAG
    CGCACTGAGA AGTTTGTGGC AGCTGTTCTC TCCAAGAATA GCCAAAGTAT AATAGCCTCC
    ATGGAGAACA CATCATCCAT ATTTGTGTGG AGGAGAGACA GTGGACAGTG CATGGCCAGC
    TTGATAGAGA TATCGGGAGC CATTGTAAAG CTAATCAAAT CAACACACCA TAACTTGCTT
    CTGTCTGTGG CCAGCAGTGG GGTGCTTTCC GTGTGGGATA TCGACATTAT AACAGCCATG
    TCGAACATTG ATAAAACTGG AAAACCTATC CAAATTCTGC AACTCTCTGG CAGAGAGGAT
    TTTGTATACA CCATGGATGG GTCAGAAGTC ATCCATAAAT GGAACTACAG CACAGGTTTC
    ATTGAAATGG TGTTCAAACA TGAAGGACTT GTTGAGAACT GTGTCCTGAC AACATCAGGT
    GACCTAATGG TGACATCTGA CGATAAAAGT AGTCAGTACA TCTGGCATAC GACCACAGGT
    GAGAATATAT TCCGTATCAA TGGCCAGAAA ATCTCACAGC TTCTCATCAC GCACAATGAC
    CAATTTGTTG TGTCACTTTG TGAGCAGAAT GCCTCTCGTG TCTGGAGGCT TGCCACTGGT
    CACAAGGTGT GCAACATTCT GGTAACACTT CAACATGCCC TCATTACTAC AGCAAACACA
    TTCCTTGTGG GTGCAAACAA GAATAAGCTG CTGGCTGTTA GCTTATGGTC AGGGAGTGTT
    TCCAAAAAGT TTATCTGTGA TGATGGGATC ACCATTGTTA ACTTCAAGTT AATCCCTGAC
    AGTCCAGATT TTGTTGTCTT CATCACCTCC ACTGAAACGG TATTCATCTG GAGTGTTGCC
    GATGAGTCAG TATGTAGACG GGTGCAACTC CCCAACAACT TCTTAAAGAA CCTGGAAGAT
    TTCCAAATTT CACCGAATGG GAAATTGGGC ATTGTCTCAA AAGGTGATGA GAACATCAAT
    GTTCTAGACC TACACAGTGG TAAGCTGCGT CTGGTGCATG CTGCTGGCAT AATATGGCGG
    CAAAAGCTTT CAAGAGATGG ACGCTACTTA GTATACATCT GTTTCCGGAA CTGCGATGAA
    GATGATGATG CAGGTGTGGT TTCAAGCCTG ATTGTCATGA GACTGGCAGA TGGTAAGAGT
    ATCGGGACCT GTTCCCTTTA CAAAACGCCA ACCTATCTGT GCCTATCACA GCGAGCACTT
    AACATCATTG TCGGATTTGA GGATGGCAGC ATTGGCACTT ATACAGTGGT TGATCGTGTA
    GATGCTGCTC TGAAGATCAA GATCGCCACC TCAAACAGTC GCCAAATTGT TAACAACGCC
    TCACAGAAAG TACGACCCAA GTGTAGCAAC AATGCCTTCA AGACCATTGC TGACTGTGTG
    TGGAGAGAAT CCACTGAGGT CTTCTCTCGA GACAGTCCTA TCAACGTGTC TGATGGTGGG
    GAGCCAGAGA CCACCACACC CACAAAGAAA ACTGAACTGC TGCAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa43788
    Clone ID Related Accession (Same CDS sequence) XM_009303553.2 , XM_009303553.3
    Accession Version XM_009303553.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 5208bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product NACHT and WD repeat domain-containing protein 2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303553.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTGGCCAT CCGGAGCGGG CAGCAGGCAG CCCTGTCCCC GTGAATCTGC ACTGCGCAGA 
    GCAGCCATCA GTGGCAACGT CCTTGCCCTC CCACCATTCC ATGTCCCCAC GGGCAGGAGT
    GTCCGGGTCT TCATCTGTGC CAACCCTGAC GATACAGAAG CAGAGAGAAA TGCTCTGAAA
    GAACATGTCT ACCCCAAACT ACGAGACTTC TGCCGAGAGA ACTATGGCAT TGAATTCCAG
    GTGGTGGACC TGTACTGGGG CATTGATCCA GAGGAGTGGG ACAGTCCGGA GCTGCAGAGA
    CTGCGGATGA AACTACTGGA AGAATGTCTG AAGACTTCAG CTGGTCCATG CTTTGTTGGG
    CTCATAGGAG AGAAGTATGG CAGCATTCGG GTCCCAGGGG AGGTGGAGTC AGCTGAGTTT
    GAGATGATTT TGGATGCGGC AGTGGAGGCG GGACTAGACA CACGGATCTT GGAGGAGTGG
    TACTGTCGAG ATGAGAACTC AGTCCCACCT GCCTACTACC TAAAACCAAA AGCTGAGATG
    CTGAAAAACT ATCAGAATTC TATGGAGTCA AACACCGTAG CAAAATCAAA GAACGACAAG
    GCATGGAGGG CAGTCTCAGA GGAAATCAAG AGGATCTTCA GAACCTCTGT TTTACAGCTG
    CAGGAGAAGG GGACCATGAA GAGTGGTCAA GCCAAGAAAT TTCTCTGCTC AGCATTAGAG
    GATGAACTGG ACTTCGCATT GGGCAAGCAG ACCCCAGCCT TTCTCAAAAA ATGTGTTTGT
    TACATTCGGA AGATATCTAA CTTTGATCGT TTCGCTAAAC TCCCCGAAAT GGCACGTTAC
    ATGGACATTG TGGTGAGCGA TGACCGTGTC ATGAGAAACC AGGAAGCCTA CGAGCGTCTG
    CTAAAGGTCA GAGACGAGTT CATTCCGGTG GTGGTGGCAG CATCCAATCT CCGAGTCTAC
    TCATCGGTCA CTCACTGTGA CATGAAACTT GGCTACTCGC AGGAGGTAGA GAGCCATTAC
    GTTGAAGGTC TGTGTAAACA GTTTTATGAA GACATGGTGG ACATCATCCA GGCGACGGTT
    CAGCAGAACT TTGACACAGA GACAGATCCT CTCTATGATG AAATTATACA ACATTTGTCG
    CTGTGCAAGA CGTTTTCATC TTTTTATGTG TACAAGAGCG AAGCCTTGGA TATAGTCCAG
    GAGTACCTGT ACCCCTCGAA AGGTGGTCGA ATAACTCCCC AAGTGGTTTA CGGAGGACCC
    TGCACTGGAA AGACCTTGTT ACTTGCTGAA GTGGCAAAAC AGGCCTATTC ATGGATACAG
    AAAGAGATGG GACCAGAAAC AGACCCAGTG GTCATTGTCA GATTCATCGG TTCCACTGAC
    TTCTCTACTG ACCTCCGCAC CCTGCTGCAG AGCATCTGTG AGCAGATTGC CATCAACTAT
    CGGTGCCTGA TCCATTTCTT ACCAAACAAG ATCCAGGAGA TGAGAGAACT GCTGGTGAAT
    TTGCTGGGGG AGTCGTCATT CCACCGACCT CTTGTCATTA TTCTAGATGC CTTGGAGCAG
    TTGTCAGACG CAGACGAGGC TCGAAAACTG TGGTGGCTCC CGGTCCACCT GCCACGTACA
    GTTCGAATAG TAGTGTCCAC CCTGCCCAAC AAGCACGGAA TACTGCAGAA GCTGCGTTGC
    CTGATACATG ATGAGGAAGC CTATGTTGAA CTAATGCAAA GAGATCGCAA AGCTTGCAGT
    CAGACCCTTA AACAGCAGCT TCTGGGTGTC AAAAGGAAGG TGACGTCTGG CCAACAGATA
    TATGTTAATG AGGCAATGGC AAAATGTACA CTTCCTATGT TTGTTAACCT CATCTACAGA
    GAGGTTGTTC ACTGGAGATC GCACAAGGAT GTGGATGACA AATCGTTGTG TTCCACAGTA
    CACGAAAGTA TTGAGCAGCT TTTCTACTCA GTTGAAAATA AGCTTGGCTC ACGCTTTGTC
    TTTCGGGCAC TGGGCTACAT CACAATGGCA CGGGCAGGCC TGACTGAGCT GGAGCTAGAG
    GATATCTTAT CTTTAGATAA CAGTGTCCTA GGGGACATCA TGGTGAGCTC TAATCTCAAA
    AGCCCACTGC GTATTTCCTA TGATTTCATA GCTCGGCTAA AAGAAGAACT TGAAGGTTAT
    TTAGTGGAGC GTCAGGTCCA TAATGTAACA CTAATGGTGT GGGCAAATCG GCATCTACAT
    CTCATAGCAC AGAAGCTGTA CCTCAGTAAC GAAGAAGACG TGCATCAAAT GCATAGCCTT
    TTGGCTGAGT ACTTTCTTGG GGCATGGGCA GGTGGCAGAA AGAAAATATT CCACTGCGAC
    AATAATCACT TTGCCTCATT GAATATCTCT CAGCATCGAA ATCCTCACAA CCAACAGAGC
    CTTCATGGCC ATGACAAAGC TTCTGCTGAC AAGCACTCAT ATGACAGACA GACACCTGAG
    CAACCCTGGG TGTTCCAATG TAATCTGCTG GAGCCGGACA TCTTCTTTGT CAACCACCGT
    AAGATGACAG AGCTCACATA CCACTTGACA AGGAGCGGCC GCACAGATGA TCTCATGTAT
    GGTGTCATCA TGAACTTCAG TTGGCTCTAC ACTATGATAA AGATAGGGCA ATTTGACAAG
    GCCCTGTCTG ACATTGACCT AGCCTACAGC TATACGCAAG AAAAGGAGCT AAAGTTTCTT
    GCAACTACAC TCCGCAGTAT AAAGCTTAAA GTGACAAAGA ATCCAGCATC ACTGTCTGCT
    GAACTACAGC AAAGGCTCCT TCCAGTGGTC ACATCCCTTC CTAAACTCAG ACACCTCCTG
    CTTGAGTGTG ACAAGGACGG CCCTAAATAC TGCTCCATCG TGCCACTTCA CTCCTCTATG
    GATGTGACCT ACAGTCCAGA GAGATTGCCG CTCAGTTCTA GCTACATGCA GATAGTTGAG
    ACTCTGCCTA CATTAGCTCC AAACATAGTC ATTGTGGCTC TTGAAGATGG GTCCGTCAGC
    ACTTGGGATG TTGAAAGCAG ACAGCTGCTA AGACAGATAG ACACAGCAAG GTCAGTTGTT
    CTTGGAATTA GACTAACCAC TGATGAGAAA TATCTGGTAG TGGCCACTAC AAAAAATACA
    CTGCTCATTT ATGACAACCA CAAGTCTTGT TTGTTGTCTG AGGTAGAGGT CAAAGGGTCC
    AAACACTGTG GGATCACTGG TGGGGTGGCA TTCATTAATG GATTTACCTT GTCCAGCCAC
    CATGCCTTAG CTTGGCTTGA GGCAAGCAAA GATGTCAGTG TGATTGATTT GCTGTACGGC
    TGGCCATTGT ATCAGTTCCA TTGCTGGTAT GAGGTAACCT GTGTCCAGTG TTCCCCAGAT
    GGAATGTATG CTTTCTGTGG ACAGTACCTC AACACAACCA CAATATTCCA CCTTGGTAGT
    GGGGATAAGC TTGCCACAAT GACTTCTGAA TTCTCAGGTG GTTTCGTGAA GTCCATTCTG
    ATTCTAGACA CAATCAATCA AATGGTGATG ATTGACAATG AGGGCAGTCT CTCCGTATGG
    AGCACCAAAG ATATCACAAA CCCCAAGCTA ATGGAGGATT ATGACTGCAG AGGAGATGAT
    AGTGAGGTGG TGGGCATAGA GCTCTCAGAG GATCAGCGAT CCATTCTAAT CTGCAAAGCA
    AGAAGCATTG AGGTTCTGGA CACCAAAATT TGGAAGATGG CTGAAAAGTT TAAGGCCAAG
    CGCACTGAGA AGTTTGTGGC AGCTGTTCTC TCCAAGAATA GCCAAAGTAT AATAGCCTCC
    ATGGAGAACA CATCATCCAT ATTTGTGTGG AGGAGAGACA GTGGACAGTG CATGGCCAGC
    TTGATAGAGA TATCGGGAGC CATTGTAAAG CTAATCAAAT CAACACACCA TAACTTGCTT
    CTGTCTGTGG CCAGCAGTGG GGTGCTTTCC GTGTGGGATA TCGACATTAT AACAGCCATG
    TCGAACATTG ATAAAACTGG AAAACCTATC CAAATTCTGC AACTCTCTGG CAGAGAGGAT
    TTTGTATACA CCATGGATGG GTCAGAAGTC ATCCATAAAT GGAACTACAG CACAGGTTTC
    ATTGAAATGG TGTTCAAACA TGAAGGACTT GTTGAGAACT GTGTCCTGAC AACATCAGGT
    GACCTAATGG TGACATCTGA CGATAAAAGT AGTCAGTACA TCTGGCATAC GACCACAGGT
    GAGAATATAT TCCGTATCAA TGGCCAGAAA ATCTCACAGC TTCTCATCAC GCACAATGAC
    CAATTTGTTG TGTCACTTTG TGAGCAGAAT GCCTCTCGTG TCTGGAGGCT TGCCACTGGT
    CACAAGGTGT GCAACATTCT GGTAACACTT CAACATGCCC TCATTACTAC AGCAAACACA
    TTCCTTGTGG GTGCAAACAA GAATAAGCTG CTGGCTGTTA GCTTATGGTC AGGGAGTGTT
    TCCAAAAAGT TTATCTGTGA TGATGGGATC ACCATTGTTA ACTTCAAGTT AATCCCTGAC
    AGTCCAGATT TTGTTGTCTT CATCACCTCC ACTGAAACGG TATTCATCTG GAGTGTTGCC
    GATGAGTCAG TATGTAGACG GGTGCAACTC CCCAACAACT TCTTAAAGAA CCTGGAAGAT
    TTCCAAATTT CACCGAATGG GAAATTGGGC ATTGTCTCAA AAGGTGATGA GAACATCAAT
    GTTCTAGACC TACACAGTGG TAAGCTGCGT CTGGTGCATG CTGCTGGCAT AATATGGCGG
    CAAAAGCTTT CAAGAGATGG ACGCTACTTA GTATACATCT GTTTCCGGAA CTGCGATGAA
    GATGATGATG CAGGTGTGGT TTCAAGCCTG ATTGTCATGA GACTGGCAGA TGGTAAGAGT
    ATCGGGACCT GTTCCCTTTA CAAAACGCCA ACCTATCTGT GCCTATCACA GCGAGCACTT
    AACATCATTG TCGGATTTGA GGATGGCAGC ATTGGCACTT ATACAGTGGT TGATCGTGTA
    GATGCTGCTC TGAAGATCAA GATCGCCACC TCAAACAGTC GCCAAATTGT TAACAACGCC
    TCACAGAAAG TACGACCCAA GTGTAGCAAC AATGCCTTCA AGACCATTGC TGACTGTGTG
    TGGAGAGAAT CCACTGAGGT CTTCTCTCGA GACAGTCCTA TCAACGTGTC TGATGGTGGG
    GAGCCAGAGA CCACCACACC CACAAAGAAA ACTGAACTGC TGCAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301828.1
    CDS441..5648
    Translation

    Target ORF information:

    RefSeq Version XM_009303553.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio NACHT and WD repeat domain containing 2 (nwd2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303553.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    ATGTGGCCAT CCGGAGCGGG CAGCAGGCAG CCCTGTCCCC GTGAATCTGC ACTGCGCAGA 
    GCAGCCATCA GTGGCAACGT CCTTGCCCTC CCACCATTCC ATGTCCCCAC GGGCAGGAGT
    GTCCGGGTCT TCATCTGTGC CAACCCTGAC GATACAGAAG CAGAGAGAAA TGCTCTGAAA
    GAACATGTCT ACCCCAAACT ACGAGACTTC TGCCGAGAGA ACTATGGCAT TGAATTCCAG
    GTGGTGGACC TGTACTGGGG CATTGATCCA GAGGAGTGGG ACAGTCCGGA GCTGCAGAGA
    CTGCGGATGA AACTACTGGA AGAATGTCTG AAGACTTCAG CTGGTCCATG CTTTGTTGGG
    CTCATAGGAG AGAAGTATGG CAGCATTCGG GTCCCAGGGG AGGTGGAGTC AGCTGAGTTT
    GAGATGATTT TGGATGCGGC AGTGGAGGCG GGACTAGACA CACGGATCTT GGAGGAGTGG
    TACTGTCGAG ATGAGAACTC AGTCCCACCT GCCTACTACC TAAAACCAAA AGCTGAGATG
    CTGAAAAACT ATCAGAATTC TATGGAGTCA AACACCGTAG CAAAATCAAA GAACGACAAG
    GCATGGAGGG CAGTCTCAGA GGAAATCAAG AGGATCTTCA GAACCTCTGT TTTACAGCTG
    CAGGAGAAGG GGACCATGAA GAGTGGTCAA GCCAAGAAAT TTCTCTGCTC AGCATTAGAG
    GATGAACTGG ACTTCGCATT GGGCAAGCAG ACCCCAGCCT TTCTCAAAAA ATGTGTTTGT
    TACATTCGGA AGATATCTAA CTTTGATCGT TTCGCTAAAC TCCCCGAAAT GGCACGTTAC
    ATGGACATTG TGGTGAGCGA TGACCGTGTC ATGAGAAACC AGGAAGCCTA CGAGCGTCTG
    CTAAAGGTCA GAGACGAGTT CATTCCGGTG GTGGTGGCAG CATCCAATCT CCGAGTCTAC
    TCATCGGTCA CTCACTGTGA CATGAAACTT GGCTACTCGC AGGAGGTAGA GAGCCATTAC
    GTTGAAGGTC TGTGTAAACA GTTTTATGAA GACATGGTGG ACATCATCCA GGCGACGGTT
    CAGCAGAACT TTGACACAGA GACAGATCCT CTCTATGATG AAATTATACA ACATTTGTCG
    CTGTGCAAGA CGTTTTCATC TTTTTATGTG TACAAGAGCG AAGCCTTGGA TATAGTCCAG
    GAGTACCTGT ACCCCTCGAA AGGTGGTCGA ATAACTCCCC AAGTGGTTTA CGGAGGACCC
    TGCACTGGAA AGACCTTGTT ACTTGCTGAA GTGGCAAAAC AGGCCTATTC ATGGATACAG
    AAAGAGATGG GACCAGAAAC AGACCCAGTG GTCATTGTCA GATTCATCGG TTCCACTGAC
    TTCTCTACTG ACCTCCGCAC CCTGCTGCAG AGCATCTGTG AGCAGATTGC CATCAACTAT
    CGGTGCCTGA TCCATTTCTT ACCAAACAAG ATCCAGGAGA TGAGAGAACT GCTGGTGAAT
    TTGCTGGGGG AGTCGTCATT CCACCGACCT CTTGTCATTA TTCTAGATGC CTTGGAGCAG
    TTGTCAGACG CAGACGAGGC TCGAAAACTG TGGTGGCTCC CGGTCCACCT GCCACGTACA
    GTTCGAATAG TAGTGTCCAC CCTGCCCAAC AAGCACGGAA TACTGCAGAA GCTGCGTTGC
    CTGATACATG ATGAGGAAGC CTATGTTGAA CTAATGCAAA GAGATCGCAA AGCTTGCAGT
    CAGACCCTTA AACAGCAGCT TCTGGGTGTC AAAAGGAAGG TGACGTCTGG CCAACAGATA
    TATGTTAATG AGGCAATGGC AAAATGTACA CTTCCTATGT TTGTTAACCT CATCTACAGA
    GAGGTTGTTC ACTGGAGATC GCACAAGGAT GTGGATGACA AATCGTTGTG TTCCACAGTA
    CACGAAAGTA TTGAGCAGCT TTTCTACTCA GTTGAAAATA AGCTTGGCTC ACGCTTTGTC
    TTTCGGGCAC TGGGCTACAT CACAATGGCA CGGGCAGGCC TGACTGAGCT GGAGCTAGAG
    GATATCTTAT CTTTAGATAA CAGTGTCCTA GGGGACATCA TGGTGAGCTC TAATCTCAAA
    AGCCCACTGC GTATTTCCTA TGATTTCATA GCTCGGCTAA AAGAAGAACT TGAAGGTTAT
    TTAGTGGAGC GTCAGGTCCA TAATGTAACA CTAATGGTGT GGGCAAATCG GCATCTACAT
    CTCATAGCAC AGAAGCTGTA CCTCAGTAAC GAAGAAGACG TGCATCAAAT GCATAGCCTT
    TTGGCTGAGT ACTTTCTTGG GGCATGGGCA GGTGGCAGAA AGAAAATATT CCACTGCGAC
    AATAATCACT TTGCCTCATT GAATATCTCT CAGCATCGAA ATCCTCACAA CCAACAGAGC
    CTTCATGGCC ATGACAAAGC TTCTGCTGAC AAGCACTCAT ATGACAGACA GACACCTGAG
    CAACCCTGGG TGTTCCAATG TAATCTGCTG GAGCCGGACA TCTTCTTTGT CAACCACCGT
    AAGATGACAG AGCTCACATA CCACTTGACA AGGAGCGGCC GCACAGATGA TCTCATGTAT
    GGTGTCATCA TGAACTTCAG TTGGCTCTAC ACTATGATAA AGATAGGGCA ATTTGACAAG
    GCCCTGTCTG ACATTGACCT AGCCTACAGC TATACGCAAG AAAAGGAGCT AAAGTTTCTT
    GCAACTACAC TCCGCAGTAT AAAGCTTAAA GTGACAAAGA ATCCAGCATC ACTGTCTGCT
    GAACTACAGC AAAGGCTCCT TCCAGTGGTC ACATCCCTTC CTAAACTCAG ACACCTCCTG
    CTTGAGTGTG ACAAGGACGG CCCTAAATAC TGCTCCATCG TGCCACTTCA CTCCTCTATG
    GATGTGACCT ACAGTCCAGA GAGATTGCCG CTCAGTTCTA GCTACATGCA GATAGTTGAG
    ACTCTGCCTA CATTAGCTCC AAACATAGTC ATTGTGGCTC TTGAAGATGG GTCCGTCAGC
    ACTTGGGATG TTGAAAGCAG ACAGCTGCTA AGACAGATAG ACACAGCAAG GTCAGTTGTT
    CTTGGAATTA GACTAACCAC TGATGAGAAA TATCTGGTAG TGGCCACTAC AAAAAATACA
    CTGCTCATTT ATGACAACCA CAAGTCTTGT TTGTTGTCTG AGGTAGAGGT CAAAGGGTCC
    AAACACTGTG GGATCACTGG TGGGGTGGCA TTCATTAATG GATTTACCTT GTCCAGCCAC
    CATGCCTTAG CTTGGCTTGA GGCAAGCAAA GATGTCAGTG TGATTGATTT GCTGTACGGC
    TGGCCATTGT ATCAGTTCCA TTGCTGGTAT GAGGTAACCT GTGTCCAGTG TTCCCCAGAT
    GGAATGTATG CTTTCTGTGG ACAGTACCTC AACACAACCA CAATATTCCA CCTTGGTAGT
    GGGGATAAGC TTGCCACAAT GACTTCTGAA TTCTCAGGTG GTTTCGTGAA GTCCATTCTG
    ATTCTAGACA CAATCAATCA AATGGTGATG ATTGACAATG AGGGCAGTCT CTCCGTATGG
    AGCACCAAAG ATATCACAAA CCCCAAGCTA ATGGAGGATT ATGACTGCAG AGGAGATGAT
    AGTGAGGTGG TGGGCATAGA GCTCTCAGAG GATCAGCGAT CCATTCTAAT CTGCAAAGCA
    AGAAGCATTG AGGTTCTGGA CACCAAAATT TGGAAGATGG CTGAAAAGTT TAAGGCCAAG
    CGCACTGAGA AGTTTGTGGC AGCTGTTCTC TCCAAGAATA GCCAAAGTAT AATAGCCTCC
    ATGGAGAACA CATCATCCAT ATTTGTGTGG AGGAGAGACA GTGGACAGTG CATGGCCAGC
    TTGATAGAGA TATCGGGAGC CATTGTAAAG CTAATCAAAT CAACACACCA TAACTTGCTT
    CTGTCTGTGG CCAGCAGTGG GGTGCTTTCC GTGTGGGATA TCGACATTAT AACAGCCATG
    TCGAACATTG ATAAAACTGG AAAACCTATC CAAATTCTGC AACTCTCTGG CAGAGAGGAT
    TTTGTATACA CCATGGATGG GTCAGAAGTC ATCCATAAAT GGAACTACAG CACAGGTTTC
    ATTGAAATGG TGTTCAAACA TGAAGGACTT GTTGAGAACT GTGTCCTGAC AACATCAGGT
    GACCTAATGG TGACATCTGA CGATAAAAGT AGTCAGTACA TCTGGCATAC GACCACAGGT
    GAGAATATAT TCCGTATCAA TGGCCAGAAA ATCTCACAGC TTCTCATCAC GCACAATGAC
    CAATTTGTTG TGTCACTTTG TGAGCAGAAT GCCTCTCGTG TCTGGAGGCT TGCCACTGGT
    CACAAGGTGT GCAACATTCT GGTAACACTT CAACATGCCC TCATTACTAC AGCAAACACA
    TTCCTTGTGG GTGCAAACAA GAATAAGCTG CTGGCTGTTA GCTTATGGTC AGGGAGTGTT
    TCCAAAAAGT TTATCTGTGA TGATGGGATC ACCATTGTTA ACTTCAAGTT AATCCCTGAC
    AGTCCAGATT TTGTTGTCTT CATCACCTCC ACTGAAACGG TATTCATCTG GAGTGTTGCC
    GATGAGTCAG TATGTAGACG GGTGCAACTC CCCAACAACT TCTTAAAGAA CCTGGAAGAT
    TTCCAAATTT CACCGAATGG GAAATTGGGC ATTGTCTCAA AAGGTGATGA GAACATCAAT
    GTTCTAGACC TACACAGTGG TAAGCTGCGT CTGGTGCATG CTGCTGGCAT AATATGGCGG
    CAAAAGCTTT CAAGAGATGG ACGCTACTTA GTATACATCT GTTTCCGGAA CTGCGATGAA
    GATGATGATG CAGGTGTGGT TTCAAGCCTG ATTGTCATGA GACTGGCAGA TGGTAAGAGT
    ATCGGGACCT GTTCCCTTTA CAAAACGCCA ACCTATCTGT GCCTATCACA GCGAGCACTT
    AACATCATTG TCGGATTTGA GGATGGCAGC ATTGGCACTT ATACAGTGGT TGATCGTGTA
    GATGCTGCTC TGAAGATCAA GATCGCCACC TCAAACAGTC GCCAAATTGT TAACAACGCC
    TCACAGAAAG TACGACCCAA GTGTAGCAAC AATGCCTTCA AGACCATTGC TGACTGTGTG
    TGGAGAGAAT CCACTGAGGT CTTCTCTCGA GACAGTCCTA TCAACGTGTC TGATGGTGGG
    GAGCCAGAGA CCACCACACC CACAAAGAAA ACTGAACTGC TGCAATGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.