nwd2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	nwd2
Entrez Gene ID	562320
Full Name	NACHT and WD repeat domain containing 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009303553.2	XP_009301828.1	NACHT and WD repeat domain-containing protein 2 isoform X2
XM_005172360.2	XP_005172417.1	NACHT and WD repeat domain-containing protein 2 isoform X3
XM_685709.8	XP_690801.5	NACHT and WD repeat domain-containing protein 2 isoform X1
XM_009303553.3	XP_009301828.1	NACHT and WD repeat domain-containing protein 2 isoform X2
XM_685709.7	XP_690801.5	NACHT and WD repeat domain-containing protein 2 isoform X1
XM_005172360.3	XP_005172417.1	NACHT and WD repeat domain-containing protein 2 isoform X3

Pathways from BioSystems

Homologies

Homo sapiens (human)	KIAA1239	NP_001138462.1
Canis lupus familiaris (dog)	KIAA1239	XP_545955.3
Drosophila melanogaster (fruit fly)	CG10185	NP_650521.1
Bos taurus (cattle)	KIAA1239	XP_002688243.1
Danio rerio (zebrafish)	LOC562320	XP_690801.5
	LOC702906	XP_001091217.2
Mus musculus (house mouse)	3110047P20Rik	NP_795980.2
Gallus gallus (chicken)	KIAA1239	XP_426345.4
Xenopus tropicalis (tropical clawed frog)	kiaa1239	XP_004911280.1
Pan troglodytes (chimpanzee)	KIAA1239	XP_001137884.1
Rattus norvegicus (Norway rat)	RGD1563065	XP_223426.6
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP011163	XP_309486.3

Gene Ontology

The following nwd2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nwd2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa13555	XM_685709.8 Latest version!	Danio rerio NACHT and WD repeat domain containing 2 (nwd2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa13556	XM_005172360.3 Latest version!	Danio rerio NACHT and WD repeat domain containing 2 (nwd2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa43788	XM_009303553.3 Latest version!	Danio rerio NACHT and WD repeat domain containing 2 (nwd2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
View Old Accession Versions >>
ODa43788	XM_009303553.2	Danio rerio NACHT and WD repeat domain containing 2 (nwd2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa13555	XM_685709.7	Danio rerio NACHT and WD repeat domain containing 2 (nwd2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa13556	XM_005172360.2	Danio rerio NACHT and WD repeat domain containing 2 (nwd2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

nwd2 ( XM_685709.8 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_690801.5 Danio rerio NACHT and WD repeat domain containing 2 (nwd2), transcript variant X1, mRNA.

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CloneID	ODa13555
Clone ID Related Accession (Same CDS sequence)	XM_685709.8 , XM_685709.7
Accession Version	XM_685709.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NACHT and WD repeat domain-containing protein 2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_685709.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGGA TGAACCCACG GTGGAAGTCG CCACCCATGT GGCCATCCGG AGCGGGCAGC 
AGGCAGCCCT GTCCCCGTGA ATCTGCACTG CGCAGAGCAG CCATCAGTGG CAACGTCCTT 
GCCCTCCCAC CATTCCATGT CCCCACGGGC AGGAGTGTCC GGGTCTTCAT CTGTGCCAAC 
CCTGACGATA CAGAAGCAGA GAGAAATGCT CTGAAAGAAC ATGTCTACCC CAAACTACGA 
GACTTCTGCC GAGAGAACTA TGGCATTGAA TTCCAGGTGG TGGACCTGTA CTGGGGCATT 
GATCCAGAGG AGTGGGACAG TCCGGAGCTG CAGAGACTGC GGATGAAACT ACTGGAAGAA 
TGTCTGAAGA CTTCAGCTGG TCCATGCTTT GTTGGGCTCA TAGGAGAGAA GTATGGCAGC 
ATTCGGGTCC CAGGGGAGGT GGAGTCAGCT GAGTTTGAGA TGATTTTGGA TGCGGCAGTG 
GAGGCGGGAC TAGACACACG GATCTTGGAG GAGTGGTACT GTCGAGATGA GAACTCAGTC 
CCACCTGCCT ACTACCTAAA ACCAAAAGCT GAGATGCTGA AAAACTATCA GAATTCTATG 
GAGTCAAACA CCGTAGCAAA ATCAAAGAAC GACAAGGCAT GGAGGGCAGT CTCAGAGGAA 
ATCAAGAGGA TCTTCAGAAC CTCTGTTTTA CAGCTGCAGG AGAAGGGGAC CATGAAGAGT 
GGTCAAGCCA AGAAATTTCT CTGCTCAGCA TTAGAGGATG AACTGGACTT CGCATTGGGC 
AAGCAGACCC CAGCCTTTCT CAAAAAATGT GTTTGTTACA TTCGGAAGAT ATCTAACTTT 
GATCGTTTCG CTAAACTCCC CGAAATGGCA CGTTACATGG ACATTGTGGT GAGCGATGAC 
CGTGTCATGA GAAACCAGGA AGCCTACGAG CGTCTGCTAA AGGTCAGAGA CGAGTTCATT 
CCGGTGGTGG TGGCAGCATC CAATCTCCGA GTCTACTCAT CGGTCACTCA CTGTGACATG 
AAACTTGGCT ACTCGCAGGA GGTAGAGAGC CATTACGTTG AAGGTCTGTG TAAACAGTTT 
TATGAAGACA TGGTGGACAT CATCCAGGCG ACGGTTCAGC AGAACTTTGA CACAGAGACA 
GATCCTCTCT ATGATGAAAT TATACAACAT TTGTCGCTGT GCAAGACGTT TTCATCTTTT 
TATGTGTACA AGAGCGAAGC CTTGGATATA GTCCAGGAGT ACCTGTACCC CTCGAAAGGT 
GGTCGAATAA CTCCCCAAGT GGTTTACGGA GGACCCTGCA CTGGAAAGAC CTTGTTACTT 
GCTGAAGTGG CAAAACAGGC CTATTCATGG ATACAGAAAG AGATGGGACC AGAAACAGAC 
CCAGTGGTCA TTGTCAGATT CATCGGTTCC ACTGACTTCT CTACTGACCT CCGCACCCTG 
CTGCAGAGCA TCTGTGAGCA GATTGCCATC AACTATCGGT GCCTGATCCA TTTCTTACCA 
AACAAGATCC AGGAGATGAG AGAACTGCTG GTGAATTTGC TGGGGGAGTC GTCATTCCAC 
CGACCTCTTG TCATTATTCT AGATGCCTTG GAGCAGTTGT CAGACGCAGA CGAGGCTCGA 
AAACTGTGGT GGCTCCCGGT CCACCTGCCA CGTACAGTTC GAATAGTAGT GTCCACCCTG 
CCCAACAAGC ACGGAATACT GCAGAAGCTG CGTTGCCTGA TACATGATGA GGAAGCCTAT 
GTTGAACTAA TGCAAAGAGA TCGCAAAGCT TGCAGTCAGA CCCTTAAACA GCAGCTTCTG 
GGTGTCAAAA GGAAGGTGAC GTCTGGCCAA CAGATATATG TTAATGAGGC AATGGCAAAA 
TGTACACTTC CTATGTTTGT TAACCTCATC TACAGAGAGG TTGTTCACTG GAGATCGCAC 
AAGGATGTGG ATGACAAATC GTTGTGTTCC ACAGTACACG AAAGTATTGA GCAGCTTTTC 
TACTCAGTTG AAAATAAGCT TGGCTCACGC TTTGTCTTTC GGGCACTGGG CTACATCACA 
ATGGCACGGG CAGGCCTGAC TGAGCTGGAG CTAGAGGATA TCTTATCTTT AGATAACAGT 
GTCCTAGGGG ACATCATGGT GAGCTCTAAT CTCAAAAGCC CACTGCGTAT TTCCTATGAT 
TTCATAGCTC GGCTAAAAGA AGAACTTGAA GGTTATTTAG TGGAGCGTCA GGTCCATAAT 
GTAACACTAA TGGTGTGGGC AAATCGGCAT CTACATCTCA TAGCACAGAA GCTGTACCTC 
AGTAACGAAG AAGACGTGCA TCAAATGCAT AGCCTTTTGG CTGAGTACTT TCTTGGGGCA 
TGGGCAGGTG GCAGAAAGAA AATATTCCAC TGCGACAATA ATCACTTTGC CTCATTGAAT 
ATCTCTCAGC ATCGAAATCC TCACAACCAA CAGAGCCTTC ATGGCCATGA CAAAGCTTCT 
GCTGACAAGC ACTCATATGA CAGACAGACA CCTGAGCAAC CCTGGGTGTT CCAATGTAAT 
CTGCTGGAGC CGGACATCTT CTTTGTCAAC CACCGTAAGA TGACAGAGCT CACATACCAC 
TTGACAAGGA GCGGCCGCAC AGATGATCTC ATGTATGGTG TCATCATGAA CTTCAGTTGG 
CTCTACACTA TGATAAAGAT AGGGCAATTT GACAAGGCCC TGTCTGACAT TGACCTAGCC 
TACAGCTATA CGCAAGAAAA GGAGCTAAAG TTTCTTGCAA CTACACTCCG CAGTATAAAG 
CTTAAAGTGA CAAAGAATCC AGCATCACTG TCTGCTGAAC TACAGCAAAG GCTCCTTCCA 
GTGGTCACAT CCCTTCCTAA ACTCAGACAC CTCCTGCTTG AGTGTGACAA GGACGGCCCT 
AAATACTGCT CCATCGTGCC ACTTCACTCC TCTATGGATG TGACCTACAG TCCAGAGAGA 
TTGCCGCTCA GTTCTAGCTA CATGCAGATA GTTGAGACTC TGCCTACATT AGCTCCAAAC 
ATAGTCATTG TGGCTCTTGA AGATGGGTCC GTCAGCACTT GGGATGTTGA AAGCAGACAG 
CTGCTAAGAC AGATAGACAC AGCAAGGTCA GTTGTTCTTG GAATTAGACT AACCACTGAT 
GAGAAATATC TGGTAGTGGC CACTACAAAA AATACACTGC TCATTTATGA CAACCACAAG 
TCTTGTTTGT TGTCTGAGGT AGAGGTCAAA GGGTCCAAAC ACTGTGGGAT CACTGGTGGG 
GTGGCATTCA TTAATGGATT TACCTTGTCC AGCCACCATG CCTTAGCTTG GCTTGAGGCA 
AGCAAAGATG TCAGTGTGAT TGATTTGCTG TACGGCTGGC CATTGTATCA GTTCCATTGC 
TGGTATGAGG TAACCTGTGT CCAGTGTTCC CCAGATGGAA TGTATGCTTT CTGTGGACAG 
TACCTCAACA CAACCACAAT ATTCCACCTT GGTAGTGGGG ATAAGCTTGC CACAATGACT 
TCTGAATTCT CAGGTGGTTT CGTGAAGTCC ATTCTGATTC TAGACACAAT CAATCAAATG 
GTGATGATTG ACAATGAGGG CAGTCTCTCC GTATGGAGCA CCAAAGATAT CACAAACCCC 
AAGCTAATGG AGGATTATGA CTGCAGAGGA GATGATAGTG AGGTGGTGGG CATAGAGCTC 
TCAGAGGATC AGCGATCCAT TCTAATCTGC AAAGCAAGAA GCATTGAGGT TCTGGACACC 
AAAATTTGGA AGATGGCTGA AAAGTTTAAG GCCAAGCGCA CTGAGAAGTT TGTGGCAGCT 
GTTCTCTCCA AGAATAGCCA AAGTATAATA GCCTCCATGG AGAACACATC ATCCATATTT 
GTGTGGAGGA GAGACAGTGG ACAGTGCATG GCCAGCTTGA TAGAGATATC GGGAGCCATT 
GTAAAGCTAA TCAAATCAAC ACACCATAAC TTGCTTCTGT CTGTGGCCAG CAGTGGGGTG 
CTTTCCGTGT GGGATATCGA CATTATAACA GCCATGTCGA ACATTGATAA AACTGGAAAA 
CCTATCCAAA TTCTGCAACT CTCTGGCAGA GAGGATTTTG TATACACCAT GGATGGGTCA 
GAAGTCATCC ATAAATGGAA CTACAGCACA GGTTTCATTG AAATGGTGTT CAAACATGAA 
GGACTTGTTG AGAACTGTGT CCTGACAACA TCAGGTGACC TAATGGTGAC ATCTGACGAT 
AAAAGTAGTC AGTACATCTG GCATACGACC ACAGGTGAGA ATATATTCCG TATCAATGGC 
CAGAAAATCT CACAGCTTCT CATCACGCAC AATGACCAAT TTGTTGTGTC ACTTTGTGAG 
CAGAATGCCT CTCGTGTCTG GAGGCTTGCC ACTGGTCACA AGGTGTGCAA CATTCTGGTA 
ACACTTCAAC ATGCCCTCAT TACTACAGCA AACACATTCC TTGTGGGTGC AAACAAGAAT 
AAGCTGCTGG CTGTTAGCTT ATGGTCAGGG AGTGTTTCCA AAAAGTTTAT CTGTGATGAT 
GGGATCACCA TTGTTAACTT CAAGTTAATC CCTGACAGTC CAGATTTTGT TGTCTTCATC 
ACCTCCACTG AAACGGTATT CATCTGGAGT GTTGCCGATG AGTCAGTATG TAGACGGGTG 
CAACTCCCCA ACAACTTCTT AAAGAACCTG GAAGATTTCC AAATTTCACC GAATGGGAAA 
TTGGGCATTG TCTCAAAAGG TGATGAGAAC ATCAATGTTC TAGACCTACA CAGTGGTAAG 
CTGCGTCTGG TGCATGCTGC TGGCATAATA TGGCGGCAAA AGCTTTCAAG AGATGGACGC 
TACTTAGTAT ACATCTGTTT CCGGAACTGC GATGAAGATG ATGATGCAGG TGTGGTTTCA 
AGCCTGATTG TCATGAGACT GGCAGATGGT AAGAGTATCG GGACCTGTTC CCTTTACAAA 
ACGCCAACCT ATCTGTGCCT ATCACAGCGA GCACTTAACA TCATTGTCGG ATTTGAGGAT 
GGCAGCATTG GCACTTATAC AGTGGTTGAT CGTGTAGATG CTGCTCTGAA GATCAAGATC 
GCCACCTCAA ACAGTCGCCA AATTGTTAAC AACGCCTCAC AGAAAGTACG ACCCAAGTGT 
AGCAACAATG CCTTCAAGAC CATTGCTGAC TGTGTGTGGA GAGAATCCAC TGAGGTCTTC 
TCTCGAGACA GTCCTATCAA CGTGTCTGAT GGTGGGGAGC CAGAGACCAC CACACCCACA 
AAGAAAACTG AACTGCTGCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_690801.5
CDS	297..5540
Translation	MKRMNPRWKSPPMWPSGAGSRQPCPRESALRRAAISGNVLALPPFHVPTGRSVRVFICANPDDTEAERNALKEHVYPKLRDFCRENYGIEFQVVDLYWGIDPEEWDSPELQRLRMKLLEECLKTSAGPCFVGLIGEKYGSIRVPGEVESAEFEMILDAAVEAGLDTRILEEWYCRDENSVPPAYYLKPKAEMLKNYQNSMESNTVAKSKNDKAWRAVSEEIKRIFRTSVLQLQEKGTMKSGQAKKFLCSALEDELDFALGKQTPAFLKKCVCYIRKISNFDRFAKLPEMARYMDIVVSDDRVMRNQEAYERLLKVRDEFIPVVVAASNLRVYSSVTHCDMKLGYSQEVESHYVEGLCKQFYEDMVDIIQATVQQNFDTETDPLYDEIIQHLSLCKTFSSFYVYKSEALDIVQEYLYPSKGGRITPQVVYGGPCTGKTLLLAEVAKQAYSWIQKEMGPETDPVVIVRFIGSTDFSTDLRTLLQSICEQIAINYRCLIHFLPNKIQEMRELLVNLLGESSFHRPLVIILDALEQLSDADEARKLWWLPVHLPRTVRIVVSTLPNKHGILQKLRCLIHDEEAYVELMQRDRKACSQTLKQQLLGVKRKVTSGQQIYVNEAMAKCTLPMFVNLIYREVVHWRSHKDVDDKSLCSTVHESIEQLFYSVENKLGSRFVFRALGYITMARAGLTELELEDILSLDNSVLGDIMVSSNLKSPLRISYDFIARLKEELEGYLVERQVHNVTLMVWANRHLHLIAQKLYLSNEEDVHQMHSLLAEYFLGAWAGGRKKIFHCDNNHFASLNISQHRNPHNQQSLHGHDKASADKHSYDRQTPEQPWVFQCNLLEPDIFFVNHRKMTELTYHLTRSGRTDDLMYGVIMNFSWLYTMIKIGQFDKALSDIDLAYSYTQEKELKFLATTLRSIKLKVTKNPASLSAELQQRLLPVVTSLPKLRHLLLECDKDGPKYCSIVPLHSSMDVTYSPERLPLSSSYMQIVETLPTLAPNIVIVALEDGSVSTWDVESRQLLRQIDTARSVVLGIRLTTDEKYLVVATTKNTLLIYDNHKSCLLSEVEVKGSKHCGITGGVAFINGFTLSSHHALAWLEASKDVSVIDLLYGWPLYQFHCWYEVTCVQCSPDGMYAFCGQYLNTTTIFHLGSGDKLATMTSEFSGGFVKSILILDTINQMVMIDNEGSLSVWSTKDITNPKLMEDYDCRGDDSEVVGIELSEDQRSILICKARSIEVLDTKIWKMAEKFKAKRTEKFVAAVLSKNSQSIIASMENTSSIFVWRRDSGQCMASLIEISGAIVKLIKSTHHNLLLSVASSGVLSVWDIDIITAMSNIDKTGKPIQILQLSGREDFVYTMDGSEVIHKWNYSTGFIEMVFKHEGLVENCVLTTSGDLMVTSDDKSSQYIWHTTTGENIFRINGQKISQLLITHNDQFVVSLCEQNASRVWRLATGHKVCNILVTLQHALITTANTFLVGANKNKLLAVSLWSGSVSKKFICDDGITIVNFKLIPDSPDFVVFITSTETVFIWSVADESVCRRVQLPNNFLKNLEDFQISPNGKLGIVSKGDENINVLDLHSGKLRLVHAAGIIWRQKLSRDGRYLVYICFRNCDEDDDAGVVSSLIVMRLADGKSIGTCSLYKTPTYLCLSQRALNIIVGFEDGSIGTYTVVDRVDAALKIKIATSNSRQIVNNASQKVRPKCSNNAFKTIADCVWRESTEVFSRDSPINVSDGGEPETTTPTKKTELLQ

Target ORF information:

RefSeq Version	XM_685709.8
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NACHT and WD repeat domain containing 2 (nwd2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_685709.8

ORF Insert Sequence:

ATGAAGAGGA TGAACCCACG GTGGAAGTCG CCACCCATGT GGCCATCCGG AGCGGGCAGC 
AGGCAGCCCT GTCCCCGTGA ATCTGCACTG CGCAGAGCAG CCATCAGTGG CAACGTCCTT 
GCCCTCCCAC CATTCCATGT CCCCACGGGC AGGAGTGTCC GGGTCTTCAT CTGTGCCAAC 
CCTGACGATA CAGAAGCAGA GAGAAATGCT CTGAAAGAAC ATGTCTACCC CAAACTACGA 
GACTTCTGCC GAGAGAACTA TGGCATTGAA TTCCAGGTGG TGGACCTGTA CTGGGGCATT 
GATCCAGAGG AGTGGGACAG TCCGGAGCTG CAGAGACTGC GGATGAAACT ACTGGAAGAA 
TGTCTGAAGA CTTCAGCTGG TCCATGCTTT GTTGGGCTCA TAGGAGAGAA GTATGGCAGC 
ATTCGGGTCC CAGGGGAGGT GGAGTCAGCT GAGTTTGAGA TGATTTTGGA TGCGGCAGTG 
GAGGCGGGAC TAGACACACG GATCTTGGAG GAGTGGTACT GTCGAGATGA GAACTCAGTC 
CCACCTGCCT ACTACCTAAA ACCAAAAGCT GAGATGCTGA AAAACTATCA GAATTCTATG 
GAGTCAAACA CCGTAGCAAA ATCAAAGAAC GACAAGGCAT GGAGGGCAGT CTCAGAGGAA 
ATCAAGAGGA TCTTCAGAAC CTCTGTTTTA CAGCTGCAGG AGAAGGGGAC CATGAAGAGT 
GGTCAAGCCA AGAAATTTCT CTGCTCAGCA TTAGAGGATG AACTGGACTT CGCATTGGGC 
AAGCAGACCC CAGCCTTTCT CAAAAAATGT GTTTGTTACA TTCGGAAGAT ATCTAACTTT 
GATCGTTTCG CTAAACTCCC CGAAATGGCA CGTTACATGG ACATTGTGGT GAGCGATGAC 
CGTGTCATGA GAAACCAGGA AGCCTACGAG CGTCTGCTAA AGGTCAGAGA CGAGTTCATT 
CCGGTGGTGG TGGCAGCATC CAATCTCCGA GTCTACTCAT CGGTCACTCA CTGTGACATG 
AAACTTGGCT ACTCGCAGGA GGTAGAGAGC CATTACGTTG AAGGTCTGTG TAAACAGTTT 
TATGAAGACA TGGTGGACAT CATCCAGGCG ACGGTTCAGC AGAACTTTGA CACAGAGACA 
GATCCTCTCT ATGATGAAAT TATACAACAT TTGTCGCTGT GCAAGACGTT TTCATCTTTT 
TATGTGTACA AGAGCGAAGC CTTGGATATA GTCCAGGAGT ACCTGTACCC CTCGAAAGGT 
GGTCGAATAA CTCCCCAAGT GGTTTACGGA GGACCCTGCA CTGGAAAGAC CTTGTTACTT 
GCTGAAGTGG CAAAACAGGC CTATTCATGG ATACAGAAAG AGATGGGACC AGAAACAGAC 
CCAGTGGTCA TTGTCAGATT CATCGGTTCC ACTGACTTCT CTACTGACCT CCGCACCCTG 
CTGCAGAGCA TCTGTGAGCA GATTGCCATC AACTATCGGT GCCTGATCCA TTTCTTACCA 
AACAAGATCC AGGAGATGAG AGAACTGCTG GTGAATTTGC TGGGGGAGTC GTCATTCCAC 
CGACCTCTTG TCATTATTCT AGATGCCTTG GAGCAGTTGT CAGACGCAGA CGAGGCTCGA 
AAACTGTGGT GGCTCCCGGT CCACCTGCCA CGTACAGTTC GAATAGTAGT GTCCACCCTG 
CCCAACAAGC ACGGAATACT GCAGAAGCTG CGTTGCCTGA TACATGATGA GGAAGCCTAT 
GTTGAACTAA TGCAAAGAGA TCGCAAAGCT TGCAGTCAGA CCCTTAAACA GCAGCTTCTG 
GGTGTCAAAA GGAAGGTGAC GTCTGGCCAA CAGATATATG TTAATGAGGC AATGGCAAAA 
TGTACACTTC CTATGTTTGT TAACCTCATC TACAGAGAGG TTGTTCACTG GAGATCGCAC 
AAGGATGTGG ATGACAAATC GTTGTGTTCC ACAGTACACG AAAGTATTGA GCAGCTTTTC 
TACTCAGTTG AAAATAAGCT TGGCTCACGC TTTGTCTTTC GGGCACTGGG CTACATCACA 
ATGGCACGGG CAGGCCTGAC TGAGCTGGAG CTAGAGGATA TCTTATCTTT AGATAACAGT 
GTCCTAGGGG ACATCATGGT GAGCTCTAAT CTCAAAAGCC CACTGCGTAT TTCCTATGAT 
TTCATAGCTC GGCTAAAAGA AGAACTTGAA GGTTATTTAG TGGAGCGTCA GGTCCATAAT 
GTAACACTAA TGGTGTGGGC AAATCGGCAT CTACATCTCA TAGCACAGAA GCTGTACCTC 
AGTAACGAAG AAGACGTGCA TCAAATGCAT AGCCTTTTGG CTGAGTACTT TCTTGGGGCA 
TGGGCAGGTG GCAGAAAGAA AATATTCCAC TGCGACAATA ATCACTTTGC CTCATTGAAT 
ATCTCTCAGC ATCGAAATCC TCACAACCAA CAGAGCCTTC ATGGCCATGA CAAAGCTTCT 
GCTGACAAGC ACTCATATGA CAGACAGACA CCTGAGCAAC CCTGGGTGTT CCAATGTAAT 
CTGCTGGAGC CGGACATCTT CTTTGTCAAC CACCGTAAGA TGACAGAGCT CACATACCAC 
TTGACAAGGA GCGGCCGCAC AGATGATCTC ATGTATGGTG TCATCATGAA CTTCAGTTGG 
CTCTACACTA TGATAAAGAT AGGGCAATTT GACAAGGCCC TGTCTGACAT TGACCTAGCC 
TACAGCTATA CGCAAGAAAA GGAGCTAAAG TTTCTTGCAA CTACACTCCG CAGTATAAAG 
CTTAAAGTGA CAAAGAATCC AGCATCACTG TCTGCTGAAC TACAGCAAAG GCTCCTTCCA 
GTGGTCACAT CCCTTCCTAA ACTCAGACAC CTCCTGCTTG AGTGTGACAA GGACGGCCCT 
AAATACTGCT CCATCGTGCC ACTTCACTCC TCTATGGATG TGACCTACAG TCCAGAGAGA 
TTGCCGCTCA GTTCTAGCTA CATGCAGATA GTTGAGACTC TGCCTACATT AGCTCCAAAC 
ATAGTCATTG TGGCTCTTGA AGATGGGTCC GTCAGCACTT GGGATGTTGA AAGCAGACAG 
CTGCTAAGAC AGATAGACAC AGCAAGGTCA GTTGTTCTTG GAATTAGACT AACCACTGAT 
GAGAAATATC TGGTAGTGGC CACTACAAAA AATACACTGC TCATTTATGA CAACCACAAG 
TCTTGTTTGT TGTCTGAGGT AGAGGTCAAA GGGTCCAAAC ACTGTGGGAT CACTGGTGGG 
GTGGCATTCA TTAATGGATT TACCTTGTCC AGCCACCATG CCTTAGCTTG GCTTGAGGCA 
AGCAAAGATG TCAGTGTGAT TGATTTGCTG TACGGCTGGC CATTGTATCA GTTCCATTGC 
TGGTATGAGG TAACCTGTGT CCAGTGTTCC CCAGATGGAA TGTATGCTTT CTGTGGACAG 
TACCTCAACA CAACCACAAT ATTCCACCTT GGTAGTGGGG ATAAGCTTGC CACAATGACT 
TCTGAATTCT CAGGTGGTTT CGTGAAGTCC ATTCTGATTC TAGACACAAT CAATCAAATG 
GTGATGATTG ACAATGAGGG CAGTCTCTCC GTATGGAGCA CCAAAGATAT CACAAACCCC 
AAGCTAATGG AGGATTATGA CTGCAGAGGA GATGATAGTG AGGTGGTGGG CATAGAGCTC 
TCAGAGGATC AGCGATCCAT TCTAATCTGC AAAGCAAGAA GCATTGAGGT TCTGGACACC 
AAAATTTGGA AGATGGCTGA AAAGTTTAAG GCCAAGCGCA CTGAGAAGTT TGTGGCAGCT 
GTTCTCTCCA AGAATAGCCA AAGTATAATA GCCTCCATGG AGAACACATC ATCCATATTT 
GTGTGGAGGA GAGACAGTGG ACAGTGCATG GCCAGCTTGA TAGAGATATC GGGAGCCATT 
GTAAAGCTAA TCAAATCAAC ACACCATAAC TTGCTTCTGT CTGTGGCCAG CAGTGGGGTG 
CTTTCCGTGT GGGATATCGA CATTATAACA GCCATGTCGA ACATTGATAA AACTGGAAAA 
CCTATCCAAA TTCTGCAACT CTCTGGCAGA GAGGATTTTG TATACACCAT GGATGGGTCA 
GAAGTCATCC ATAAATGGAA CTACAGCACA GGTTTCATTG AAATGGTGTT CAAACATGAA 
GGACTTGTTG AGAACTGTGT CCTGACAACA TCAGGTGACC TAATGGTGAC ATCTGACGAT 
AAAAGTAGTC AGTACATCTG GCATACGACC ACAGGTGAGA ATATATTCCG TATCAATGGC 
CAGAAAATCT CACAGCTTCT CATCACGCAC AATGACCAAT TTGTTGTGTC ACTTTGTGAG 
CAGAATGCCT CTCGTGTCTG GAGGCTTGCC ACTGGTCACA AGGTGTGCAA CATTCTGGTA 
ACACTTCAAC ATGCCCTCAT TACTACAGCA AACACATTCC TTGTGGGTGC AAACAAGAAT 
AAGCTGCTGG CTGTTAGCTT ATGGTCAGGG AGTGTTTCCA AAAAGTTTAT CTGTGATGAT 
GGGATCACCA TTGTTAACTT CAAGTTAATC CCTGACAGTC CAGATTTTGT TGTCTTCATC 
ACCTCCACTG AAACGGTATT CATCTGGAGT GTTGCCGATG AGTCAGTATG TAGACGGGTG 
CAACTCCCCA ACAACTTCTT AAAGAACCTG GAAGATTTCC AAATTTCACC GAATGGGAAA 
TTGGGCATTG TCTCAAAAGG TGATGAGAAC ATCAATGTTC TAGACCTACA CAGTGGTAAG 
CTGCGTCTGG TGCATGCTGC TGGCATAATA TGGCGGCAAA AGCTTTCAAG AGATGGACGC 
TACTTAGTAT ACATCTGTTT CCGGAACTGC GATGAAGATG ATGATGCAGG TGTGGTTTCA 
AGCCTGATTG TCATGAGACT GGCAGATGGT AAGAGTATCG GGACCTGTTC CCTTTACAAA 
ACGCCAACCT ATCTGTGCCT ATCACAGCGA GCACTTAACA TCATTGTCGG ATTTGAGGAT 
GGCAGCATTG GCACTTATAC AGTGGTTGAT CGTGTAGATG CTGCTCTGAA GATCAAGATC 
GCCACCTCAA ACAGTCGCCA AATTGTTAAC AACGCCTCAC AGAAAGTACG ACCCAAGTGT 
AGCAACAATG CCTTCAAGAC CATTGCTGAC TGTGTGTGGA GAGAATCCAC TGAGGTCTTC 
TCTCGAGACA GTCCTATCAA CGTGTCTGAT GGTGGGGAGC CAGAGACCAC CACACCCACA 
AAGAAAACTG AACTGCTGCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nwd2 ( XM_685709.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_690801.5 Danio rerio NACHT and WD repeat domain containing 2 (nwd2), transcript variant X1, mRNA.

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CloneID	ODa13555
Clone ID Related Accession (Same CDS sequence)	XM_685709.8 , XM_685709.7
Accession Version	XM_685709.7	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5244bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	NACHT and WD repeat domain-containing protein 2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_685709.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGAGGA TGAACCCACG GTGGAAGTCG CCACCCATGT GGCCATCCGG AGCGGGCAGC 
AGGCAGCCCT GTCCCCGTGA ATCTGCACTG CGCAGAGCAG CCATCAGTGG CAACGTCCTT 
GCCCTCCCAC CATTCCATGT CCCCACGGGC AGGAGTGTCC GGGTCTTCAT CTGTGCCAAC 
CCTGACGATA CAGAAGCAGA GAGAAATGCT CTGAAAGAAC ATGTCTACCC CAAACTACGA 
GACTTCTGCC GAGAGAACTA TGGCATTGAA TTCCAGGTGG TGGACCTGTA CTGGGGCATT 
GATCCAGAGG AGTGGGACAG TCCGGAGCTG CAGAGACTGC GGATGAAACT ACTGGAAGAA 
TGTCTGAAGA CTTCAGCTGG TCCATGCTTT GTTGGGCTCA TAGGAGAGAA GTATGGCAGC 
ATTCGGGTCC CAGGGGAGGT GGAGTCAGCT GAGTTTGAGA TGATTTTGGA TGCGGCAGTG 
GAGGCGGGAC TAGACACACG GATCTTGGAG GAGTGGTACT GTCGAGATGA GAACTCAGTC 
CCACCTGCCT ACTACCTAAA ACCAAAAGCT GAGATGCTGA AAAACTATCA GAATTCTATG 
GAGTCAAACA CCGTAGCAAA ATCAAAGAAC GACAAGGCAT GGAGGGCAGT CTCAGAGGAA 
ATCAAGAGGA TCTTCAGAAC CTCTGTTTTA CAGCTGCAGG AGAAGGGGAC CATGAAGAGT 
GGTCAAGCCA AGAAATTTCT CTGCTCAGCA TTAGAGGATG AACTGGACTT CGCATTGGGC 
AAGCAGACCC CAGCCTTTCT CAAAAAATGT GTTTGTTACA TTCGGAAGAT ATCTAACTTT 
GATCGTTTCG CTAAACTCCC CGAAATGGCA CGTTACATGG ACATTGTGGT GAGCGATGAC 
CGTGTCATGA GAAACCAGGA AGCCTACGAG CGTCTGCTAA AGGTCAGAGA CGAGTTCATT 
CCGGTGGTGG TGGCAGCATC CAATCTCCGA GTCTACTCAT CGGTCACTCA CTGTGACATG 
AAACTTGGCT ACTCGCAGGA GGTAGAGAGC CATTACGTTG AAGGTCTGTG TAAACAGTTT 
TATGAAGACA TGGTGGACAT CATCCAGGCG ACGGTTCAGC AGAACTTTGA CACAGAGACA 
GATCCTCTCT ATGATGAAAT TATACAACAT TTGTCGCTGT GCAAGACGTT TTCATCTTTT 
TATGTGTACA AGAGCGAAGC CTTGGATATA GTCCAGGAGT ACCTGTACCC CTCGAAAGGT 
GGTCGAATAA CTCCCCAAGT GGTTTACGGA GGACCCTGCA CTGGAAAGAC CTTGTTACTT 
GCTGAAGTGG CAAAACAGGC CTATTCATGG ATACAGAAAG AGATGGGACC AGAAACAGAC 
CCAGTGGTCA TTGTCAGATT CATCGGTTCC ACTGACTTCT CTACTGACCT CCGCACCCTG 
CTGCAGAGCA TCTGTGAGCA GATTGCCATC AACTATCGGT GCCTGATCCA TTTCTTACCA 
AACAAGATCC AGGAGATGAG AGAACTGCTG GTGAATTTGC TGGGGGAGTC GTCATTCCAC 
CGACCTCTTG TCATTATTCT AGATGCCTTG GAGCAGTTGT CAGACGCAGA CGAGGCTCGA 
AAACTGTGGT GGCTCCCGGT CCACCTGCCA CGTACAGTTC GAATAGTAGT GTCCACCCTG 
CCCAACAAGC ACGGAATACT GCAGAAGCTG CGTTGCCTGA TACATGATGA GGAAGCCTAT 
GTTGAACTAA TGCAAAGAGA TCGCAAAGCT TGCAGTCAGA CCCTTAAACA GCAGCTTCTG 
GGTGTCAAAA GGAAGGTGAC GTCTGGCCAA CAGATATATG TTAATGAGGC AATGGCAAAA 
TGTACACTTC CTATGTTTGT TAACCTCATC TACAGAGAGG TTGTTCACTG GAGATCGCAC 
AAGGATGTGG ATGACAAATC GTTGTGTTCC ACAGTACACG AAAGTATTGA GCAGCTTTTC 
TACTCAGTTG AAAATAAGCT TGGCTCACGC TTTGTCTTTC GGGCACTGGG CTACATCACA 
ATGGCACGGG CAGGCCTGAC TGAGCTGGAG CTAGAGGATA TCTTATCTTT AGATAACAGT 
GTCCTAGGGG ACATCATGGT GAGCTCTAAT CTCAAAAGCC CACTGCGTAT TTCCTATGAT 
TTCATAGCTC GGCTAAAAGA AGAACTTGAA GGTTATTTAG TGGAGCGTCA GGTCCATAAT 
GTAACACTAA TGGTGTGGGC AAATCGGCAT CTACATCTCA TAGCACAGAA GCTGTACCTC 
AGTAACGAAG AAGACGTGCA TCAAATGCAT AGCCTTTTGG CTGAGTACTT TCTTGGGGCA 
TGGGCAGGTG GCAGAAAGAA AATATTCCAC TGCGACAATA ATCACTTTGC CTCATTGAAT 
ATCTCTCAGC ATCGAAATCC TCACAACCAA CAGAGCCTTC ATGGCCATGA CAAAGCTTCT 
GCTGACAAGC ACTCATATGA CAGACAGACA CCTGAGCAAC CCTGGGTGTT CCAATGTAAT 
CTGCTGGAGC CGGACATCTT CTTTGTCAAC CACCGTAAGA TGACAGAGCT CACATACCAC 
TTGACAAGGA GCGGCCGCAC AGATGATCTC ATGTATGGTG TCATCATGAA CTTCAGTTGG 
CTCTACACTA TGATAAAGAT AGGGCAATTT GACAAGGCCC TGTCTGACAT TGACCTAGCC 
TACAGCTATA CGCAAGAAAA GGAGCTAAAG TTTCTTGCAA CTACACTCCG CAGTATAAAG 
CTTAAAGTGA CAAAGAATCC AGCATCACTG TCTGCTGAAC TACAGCAAAG GCTCCTTCCA 
GTGGTCACAT CCCTTCCTAA ACTCAGACAC CTCCTGCTTG AGTGTGACAA GGACGGCCCT 
AAATACTGCT CCATCGTGCC ACTTCACTCC TCTATGGATG TGACCTACAG TCCAGAGAGA 
TTGCCGCTCA GTTCTAGCTA CATGCAGATA GTTGAGACTC TGCCTACATT AGCTCCAAAC 
ATAGTCATTG TGGCTCTTGA AGATGGGTCC GTCAGCACTT GGGATGTTGA AAGCAGACAG 
CTGCTAAGAC AGATAGACAC AGCAAGGTCA GTTGTTCTTG GAATTAGACT AACCACTGAT 
GAGAAATATC TGGTAGTGGC CACTACAAAA AATACACTGC TCATTTATGA CAACCACAAG 
TCTTGTTTGT TGTCTGAGGT AGAGGTCAAA GGGTCCAAAC ACTGTGGGAT CACTGGTGGG 
GTGGCATTCA TTAATGGATT TACCTTGTCC AGCCACCATG CCTTAGCTTG GCTTGAGGCA 
AGCAAAGATG TCAGTGTGAT TGATTTGCTG TACGGCTGGC CATTGTATCA GTTCCATTGC 
TGGTATGAGG TAACCTGTGT CCAGTGTTCC CCAGATGGAA TGTATGCTTT CTGTGGACAG 
TACCTCAACA CAACCACAAT ATTCCACCTT GGTAGTGGGG ATAAGCTTGC CACAATGACT 
TCTGAATTCT CAGGTGGTTT CGTGAAGTCC ATTCTGATTC TAGACACAAT CAATCAAATG 
GTGATGATTG ACAATGAGGG CAGTCTCTCC GTATGGAGCA CCAAAGATAT CACAAACCCC 
AAGCTAATGG AGGATTATGA CTGCAGAGGA GATGATAGTG AGGTGGTGGG CATAGAGCTC 
TCAGAGGATC AGCGATCCAT TCTAATCTGC AAAGCAAGAA GCATTGAGGT TCTGGACACC 
AAAATTTGGA AGATGGCTGA AAAGTTTAAG GCCAAGCGCA CTGAGAAGTT TGTGGCAGCT 
GTTCTCTCCA AGAATAGCCA AAGTATAATA GCCTCCATGG AGAACACATC ATCCATATTT 
GTGTGGAGGA GAGACAGTGG ACAGTGCATG GCCAGCTTGA TAGAGATATC GGGAGCCATT 
GTAAAGCTAA TCAAATCAAC ACACCATAAC TTGCTTCTGT CTGTGGCCAG CAGTGGGGTG 
CTTTCCGTGT GGGATATCGA CATTATAACA GCCATGTCGA ACATTGATAA AACTGGAAAA 
CCTATCCAAA TTCTGCAACT CTCTGGCAGA GAGGATTTTG TATACACCAT GGATGGGTCA 
GAAGTCATCC ATAAATGGAA CTACAGCACA GGTTTCATTG AAATGGTGTT CAAACATGAA 
GGACTTGTTG AGAACTGTGT CCTGACAACA TCAGGTGACC TAATGGTGAC ATCTGACGAT 
AAAAGTAGTC AGTACATCTG GCATACGACC ACAGGTGAGA ATATATTCCG TATCAATGGC 
CAGAAAATCT CACAGCTTCT CATCACGCAC AATGACCAAT TTGTTGTGTC ACTTTGTGAG 
CAGAATGCCT CTCGTGTCTG GAGGCTTGCC ACTGGTCACA AGGTGTGCAA CATTCTGGTA 
ACACTTCAAC ATGCCCTCAT TACTACAGCA AACACATTCC TTGTGGGTGC AAACAAGAAT 
AAGCTGCTGG CTGTTAGCTT ATGGTCAGGG AGTGTTTCCA AAAAGTTTAT CTGTGATGAT 
GGGATCACCA TTGTTAACTT CAAGTTAATC CCTGACAGTC CAGATTTTGT TGTCTTCATC 
ACCTCCACTG AAACGGTATT CATCTGGAGT GTTGCCGATG AGTCAGTATG TAGACGGGTG 
CAACTCCCCA ACAACTTCTT AAAGAACCTG GAAGATTTCC AAATTTCACC GAATGGGAAA 
TTGGGCATTG TCTCAAAAGG TGATGAGAAC ATCAATGTTC TAGACCTACA CAGTGGTAAG 
CTGCGTCTGG TGCATGCTGC TGGCATAATA TGGCGGCAAA AGCTTTCAAG AGATGGACGC 
TACTTAGTAT ACATCTGTTT CCGGAACTGC GATGAAGATG ATGATGCAGG TGTGGTTTCA 
AGCCTGATTG TCATGAGACT GGCAGATGGT AAGAGTATCG GGACCTGTTC CCTTTACAAA 
ACGCCAACCT ATCTGTGCCT ATCACAGCGA GCACTTAACA TCATTGTCGG ATTTGAGGAT 
GGCAGCATTG GCACTTATAC AGTGGTTGAT CGTGTAGATG CTGCTCTGAA GATCAAGATC 
GCCACCTCAA ACAGTCGCCA AATTGTTAAC AACGCCTCAC AGAAAGTACG ACCCAAGTGT 
AGCAACAATG CCTTCAAGAC CATTGCTGAC TGTGTGTGGA GAGAATCCAC TGAGGTCTTC 
TCTCGAGACA GTCCTATCAA CGTGTCTGAT GGTGGGGAGC CAGAGACCAC CACACCCACA 
AAGAAAACTG AACTGCTGCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_690801.5
CDS	270..5513
Translation	MKRMNPRWKSPPMWPSGAGSRQPCPRESALRRAAISGNVLALPPFHVPTGRSVRVFICANPDDTEAERNALKEHVYPKLRDFCRENYGIEFQVVDLYWGIDPEEWDSPELQRLRMKLLEECLKTSAGPCFVGLIGEKYGSIRVPGEVESAEFEMILDAAVEAGLDTRILEEWYCRDENSVPPAYYLKPKAEMLKNYQNSMESNTVAKSKNDKAWRAVSEEIKRIFRTSVLQLQEKGTMKSGQAKKFLCSALEDELDFALGKQTPAFLKKCVCYIRKISNFDRFAKLPEMARYMDIVVSDDRVMRNQEAYERLLKVRDEFIPVVVAASNLRVYSSVTHCDMKLGYSQEVESHYVEGLCKQFYEDMVDIIQATVQQNFDTETDPLYDEIIQHLSLCKTFSSFYVYKSEALDIVQEYLYPSKGGRITPQVVYGGPCTGKTLLLAEVAKQAYSWIQKEMGPETDPVVIVRFIGSTDFSTDLRTLLQSICEQIAINYRCLIHFLPNKIQEMRELLVNLLGESSFHRPLVIILDALEQLSDADEARKLWWLPVHLPRTVRIVVSTLPNKHGILQKLRCLIHDEEAYVELMQRDRKACSQTLKQQLLGVKRKVTSGQQIYVNEAMAKCTLPMFVNLIYREVVHWRSHKDVDDKSLCSTVHESIEQLFYSVENKLGSRFVFRALGYITMARAGLTELELEDILSLDNSVLGDIMVSSNLKSPLRISYDFIARLKEELEGYLVERQVHNVTLMVWANRHLHLIAQKLYLSNEEDVHQMHSLLAEYFLGAWAGGRKKIFHCDNNHFASLNISQHRNPHNQQSLHGHDKASADKHSYDRQTPEQPWVFQCNLLEPDIFFVNHRKMTELTYHLTRSGRTDDLMYGVIMNFSWLYTMIKIGQFDKALSDIDLAYSYTQEKELKFLATTLRSIKLKVTKNPASLSAELQQRLLPVVTSLPKLRHLLLECDKDGPKYCSIVPLHSSMDVTYSPERLPLSSSYMQIVETLPTLAPNIVIVALEDGSVSTWDVESRQLLRQIDTARSVVLGIRLTTDEKYLVVATTKNTLLIYDNHKSCLLSEVEVKGSKHCGITGGVAFINGFTLSSHHALAWLEASKDVSVIDLLYGWPLYQFHCWYEVTCVQCSPDGMYAFCGQYLNTTTIFHLGSGDKLATMTSEFSGGFVKSILILDTINQMVMIDNEGSLSVWSTKDITNPKLMEDYDCRGDDSEVVGIELSEDQRSILICKARSIEVLDTKIWKMAEKFKAKRTEKFVAAVLSKNSQSIIASMENTSSIFVWRRDSGQCMASLIEISGAIVKLIKSTHHNLLLSVASSGVLSVWDIDIITAMSNIDKTGKPIQILQLSGREDFVYTMDGSEVIHKWNYSTGFIEMVFKHEGLVENCVLTTSGDLMVTSDDKSSQYIWHTTTGENIFRINGQKISQLLITHNDQFVVSLCEQNASRVWRLATGHKVCNILVTLQHALITTANTFLVGANKNKLLAVSLWSGSVSKKFICDDGITIVNFKLIPDSPDFVVFITSTETVFIWSVADESVCRRVQLPNNFLKNLEDFQISPNGKLGIVSKGDENINVLDLHSGKLRLVHAAGIIWRQKLSRDGRYLVYICFRNCDEDDDAGVVSSLIVMRLADGKSIGTCSLYKTPTYLCLSQRALNIIVGFEDGSIGTYTVVDRVDAALKIKIATSNSRQIVNNASQKVRPKCSNNAFKTIADCVWRESTEVFSRDSPINVSDGGEPETTTPTKKTELLQ

Target ORF information:

RefSeq Version	XM_685709.7
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NACHT and WD repeat domain containing 2 (nwd2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_685709.7

ORF Insert Sequence:

ATGAAGAGGA TGAACCCACG GTGGAAGTCG CCACCCATGT GGCCATCCGG AGCGGGCAGC 
AGGCAGCCCT GTCCCCGTGA ATCTGCACTG CGCAGAGCAG CCATCAGTGG CAACGTCCTT 
GCCCTCCCAC CATTCCATGT CCCCACGGGC AGGAGTGTCC GGGTCTTCAT CTGTGCCAAC 
CCTGACGATA CAGAAGCAGA GAGAAATGCT CTGAAAGAAC ATGTCTACCC CAAACTACGA 
GACTTCTGCC GAGAGAACTA TGGCATTGAA TTCCAGGTGG TGGACCTGTA CTGGGGCATT 
GATCCAGAGG AGTGGGACAG TCCGGAGCTG CAGAGACTGC GGATGAAACT ACTGGAAGAA 
TGTCTGAAGA CTTCAGCTGG TCCATGCTTT GTTGGGCTCA TAGGAGAGAA GTATGGCAGC 
ATTCGGGTCC CAGGGGAGGT GGAGTCAGCT GAGTTTGAGA TGATTTTGGA TGCGGCAGTG 
GAGGCGGGAC TAGACACACG GATCTTGGAG GAGTGGTACT GTCGAGATGA GAACTCAGTC 
CCACCTGCCT ACTACCTAAA ACCAAAAGCT GAGATGCTGA AAAACTATCA GAATTCTATG 
GAGTCAAACA CCGTAGCAAA ATCAAAGAAC GACAAGGCAT GGAGGGCAGT CTCAGAGGAA 
ATCAAGAGGA TCTTCAGAAC CTCTGTTTTA CAGCTGCAGG AGAAGGGGAC CATGAAGAGT 
GGTCAAGCCA AGAAATTTCT CTGCTCAGCA TTAGAGGATG AACTGGACTT CGCATTGGGC 
AAGCAGACCC CAGCCTTTCT CAAAAAATGT GTTTGTTACA TTCGGAAGAT ATCTAACTTT 
GATCGTTTCG CTAAACTCCC CGAAATGGCA CGTTACATGG ACATTGTGGT GAGCGATGAC 
CGTGTCATGA GAAACCAGGA AGCCTACGAG CGTCTGCTAA AGGTCAGAGA CGAGTTCATT 
CCGGTGGTGG TGGCAGCATC CAATCTCCGA GTCTACTCAT CGGTCACTCA CTGTGACATG 
AAACTTGGCT ACTCGCAGGA GGTAGAGAGC CATTACGTTG AAGGTCTGTG TAAACAGTTT 
TATGAAGACA TGGTGGACAT CATCCAGGCG ACGGTTCAGC AGAACTTTGA CACAGAGACA 
GATCCTCTCT ATGATGAAAT TATACAACAT TTGTCGCTGT GCAAGACGTT TTCATCTTTT 
TATGTGTACA AGAGCGAAGC CTTGGATATA GTCCAGGAGT ACCTGTACCC CTCGAAAGGT 
GGTCGAATAA CTCCCCAAGT GGTTTACGGA GGACCCTGCA CTGGAAAGAC CTTGTTACTT 
GCTGAAGTGG CAAAACAGGC CTATTCATGG ATACAGAAAG AGATGGGACC AGAAACAGAC 
CCAGTGGTCA TTGTCAGATT CATCGGTTCC ACTGACTTCT CTACTGACCT CCGCACCCTG 
CTGCAGAGCA TCTGTGAGCA GATTGCCATC AACTATCGGT GCCTGATCCA TTTCTTACCA 
AACAAGATCC AGGAGATGAG AGAACTGCTG GTGAATTTGC TGGGGGAGTC GTCATTCCAC 
CGACCTCTTG TCATTATTCT AGATGCCTTG GAGCAGTTGT CAGACGCAGA CGAGGCTCGA 
AAACTGTGGT GGCTCCCGGT CCACCTGCCA CGTACAGTTC GAATAGTAGT GTCCACCCTG 
CCCAACAAGC ACGGAATACT GCAGAAGCTG CGTTGCCTGA TACATGATGA GGAAGCCTAT 
GTTGAACTAA TGCAAAGAGA TCGCAAAGCT TGCAGTCAGA CCCTTAAACA GCAGCTTCTG 
GGTGTCAAAA GGAAGGTGAC GTCTGGCCAA CAGATATATG TTAATGAGGC AATGGCAAAA 
TGTACACTTC CTATGTTTGT TAACCTCATC TACAGAGAGG TTGTTCACTG GAGATCGCAC 
AAGGATGTGG ATGACAAATC GTTGTGTTCC ACAGTACACG AAAGTATTGA GCAGCTTTTC 
TACTCAGTTG AAAATAAGCT TGGCTCACGC TTTGTCTTTC GGGCACTGGG CTACATCACA 
ATGGCACGGG CAGGCCTGAC TGAGCTGGAG CTAGAGGATA TCTTATCTTT AGATAACAGT 
GTCCTAGGGG ACATCATGGT GAGCTCTAAT CTCAAAAGCC CACTGCGTAT TTCCTATGAT 
TTCATAGCTC GGCTAAAAGA AGAACTTGAA GGTTATTTAG TGGAGCGTCA GGTCCATAAT 
GTAACACTAA TGGTGTGGGC AAATCGGCAT CTACATCTCA TAGCACAGAA GCTGTACCTC 
AGTAACGAAG AAGACGTGCA TCAAATGCAT AGCCTTTTGG CTGAGTACTT TCTTGGGGCA 
TGGGCAGGTG GCAGAAAGAA AATATTCCAC TGCGACAATA ATCACTTTGC CTCATTGAAT 
ATCTCTCAGC ATCGAAATCC TCACAACCAA CAGAGCCTTC ATGGCCATGA CAAAGCTTCT 
GCTGACAAGC ACTCATATGA CAGACAGACA CCTGAGCAAC CCTGGGTGTT CCAATGTAAT 
CTGCTGGAGC CGGACATCTT CTTTGTCAAC CACCGTAAGA TGACAGAGCT CACATACCAC 
TTGACAAGGA GCGGCCGCAC AGATGATCTC ATGTATGGTG TCATCATGAA CTTCAGTTGG 
CTCTACACTA TGATAAAGAT AGGGCAATTT GACAAGGCCC TGTCTGACAT TGACCTAGCC 
TACAGCTATA CGCAAGAAAA GGAGCTAAAG TTTCTTGCAA CTACACTCCG CAGTATAAAG 
CTTAAAGTGA CAAAGAATCC AGCATCACTG TCTGCTGAAC TACAGCAAAG GCTCCTTCCA 
GTGGTCACAT CCCTTCCTAA ACTCAGACAC CTCCTGCTTG AGTGTGACAA GGACGGCCCT 
AAATACTGCT CCATCGTGCC ACTTCACTCC TCTATGGATG TGACCTACAG TCCAGAGAGA 
TTGCCGCTCA GTTCTAGCTA CATGCAGATA GTTGAGACTC TGCCTACATT AGCTCCAAAC 
ATAGTCATTG TGGCTCTTGA AGATGGGTCC GTCAGCACTT GGGATGTTGA AAGCAGACAG 
CTGCTAAGAC AGATAGACAC AGCAAGGTCA GTTGTTCTTG GAATTAGACT AACCACTGAT 
GAGAAATATC TGGTAGTGGC CACTACAAAA AATACACTGC TCATTTATGA CAACCACAAG 
TCTTGTTTGT TGTCTGAGGT AGAGGTCAAA GGGTCCAAAC ACTGTGGGAT CACTGGTGGG 
GTGGCATTCA TTAATGGATT TACCTTGTCC AGCCACCATG CCTTAGCTTG GCTTGAGGCA 
AGCAAAGATG TCAGTGTGAT TGATTTGCTG TACGGCTGGC CATTGTATCA GTTCCATTGC 
TGGTATGAGG TAACCTGTGT CCAGTGTTCC CCAGATGGAA TGTATGCTTT CTGTGGACAG 
TACCTCAACA CAACCACAAT ATTCCACCTT GGTAGTGGGG ATAAGCTTGC CACAATGACT 
TCTGAATTCT CAGGTGGTTT CGTGAAGTCC ATTCTGATTC TAGACACAAT CAATCAAATG 
GTGATGATTG ACAATGAGGG CAGTCTCTCC GTATGGAGCA CCAAAGATAT CACAAACCCC 
AAGCTAATGG AGGATTATGA CTGCAGAGGA GATGATAGTG AGGTGGTGGG CATAGAGCTC 
TCAGAGGATC AGCGATCCAT TCTAATCTGC AAAGCAAGAA GCATTGAGGT TCTGGACACC 
AAAATTTGGA AGATGGCTGA AAAGTTTAAG GCCAAGCGCA CTGAGAAGTT TGTGGCAGCT 
GTTCTCTCCA AGAATAGCCA AAGTATAATA GCCTCCATGG AGAACACATC ATCCATATTT 
GTGTGGAGGA GAGACAGTGG ACAGTGCATG GCCAGCTTGA TAGAGATATC GGGAGCCATT 
GTAAAGCTAA TCAAATCAAC ACACCATAAC TTGCTTCTGT CTGTGGCCAG CAGTGGGGTG 
CTTTCCGTGT GGGATATCGA CATTATAACA GCCATGTCGA ACATTGATAA AACTGGAAAA 
CCTATCCAAA TTCTGCAACT CTCTGGCAGA GAGGATTTTG TATACACCAT GGATGGGTCA 
GAAGTCATCC ATAAATGGAA CTACAGCACA GGTTTCATTG AAATGGTGTT CAAACATGAA 
GGACTTGTTG AGAACTGTGT CCTGACAACA TCAGGTGACC TAATGGTGAC ATCTGACGAT 
AAAAGTAGTC AGTACATCTG GCATACGACC ACAGGTGAGA ATATATTCCG TATCAATGGC 
CAGAAAATCT CACAGCTTCT CATCACGCAC AATGACCAAT TTGTTGTGTC ACTTTGTGAG 
CAGAATGCCT CTCGTGTCTG GAGGCTTGCC ACTGGTCACA AGGTGTGCAA CATTCTGGTA 
ACACTTCAAC ATGCCCTCAT TACTACAGCA AACACATTCC TTGTGGGTGC AAACAAGAAT 
AAGCTGCTGG CTGTTAGCTT ATGGTCAGGG AGTGTTTCCA AAAAGTTTAT CTGTGATGAT 
GGGATCACCA TTGTTAACTT CAAGTTAATC CCTGACAGTC CAGATTTTGT TGTCTTCATC 
ACCTCCACTG AAACGGTATT CATCTGGAGT GTTGCCGATG AGTCAGTATG TAGACGGGTG 
CAACTCCCCA ACAACTTCTT AAAGAACCTG GAAGATTTCC AAATTTCACC GAATGGGAAA 
TTGGGCATTG TCTCAAAAGG TGATGAGAAC ATCAATGTTC TAGACCTACA CAGTGGTAAG 
CTGCGTCTGG TGCATGCTGC TGGCATAATA TGGCGGCAAA AGCTTTCAAG AGATGGACGC 
TACTTAGTAT ACATCTGTTT CCGGAACTGC GATGAAGATG ATGATGCAGG TGTGGTTTCA 
AGCCTGATTG TCATGAGACT GGCAGATGGT AAGAGTATCG GGACCTGTTC CCTTTACAAA 
ACGCCAACCT ATCTGTGCCT ATCACAGCGA GCACTTAACA TCATTGTCGG ATTTGAGGAT 
GGCAGCATTG GCACTTATAC AGTGGTTGAT CGTGTAGATG CTGCTCTGAA GATCAAGATC 
GCCACCTCAA ACAGTCGCCA AATTGTTAAC AACGCCTCAC AGAAAGTACG ACCCAAGTGT 
AGCAACAATG CCTTCAAGAC CATTGCTGAC TGTGTGTGGA GAGAATCCAC TGAGGTCTTC 
TCTCGAGACA GTCCTATCAA CGTGTCTGAT GGTGGGGAGC CAGAGACCAC CACACCCACA 
AAGAAAACTG AACTGCTGCA ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nwd2 ( XM_005172360.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172417.1 Danio rerio NACHT and WD repeat domain containing 2 (nwd2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa13556
Clone ID Related Accession (Same CDS sequence)	XM_005172360.2 , XM_005172360.3
Accession Version	XM_005172360.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	NACHT and WD repeat domain-containing protein 2 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Sep 24, 2014 this sequence version replaced XM_005172360.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTTGG ATGCGGCAGT GGAGGCGGGA CTAGACACAC GGATCTTGGA GGAGTGGTAC 
TGTCGAGATG AGAACTCAGT CCCACCTGCC TACTACCTAA AACCAAAAGC TGAGATGCTG 
AAAAACTATC AGAATTCTAT GGAGTCAAAC ACCGTAGCAA AATCAAAGAA CGACAAGGCA 
TGGAGGGCAG TCTCAGAGGA AATCAAGAGG ATCTTCAGAA CCTCTGTTTT ACAGCTGCAG 
GAGAAGGGGA CCATGAAGAG TGGTCAAGCC AAGAAATTTC TCTGCTCAGC ATTAGAGGAT 
GAACTGGACT TCGCATTGGG CAAGCAGACC CCAGCCTTTC TCAAAAAATG TGTTTGTTAC 
ATTCGGAAGA TATCTAACTT TGATCGTTTC GCTAAACTCC CCGAAATGGC ACGTTACATG 
GACATTGTGG TGAGCGATGA CCGTGTCATG AGAAACCAGG AAGCCTACGA GCGTCTGCTA 
AAGGTCAGAG ACGAGTTCAT TCCGGTGGTG GTGGCAGCAT CCAATCTCCG AGTCTACTCA 
TCGGTCACTC ACTGTGACAT GAAACTTGGC TACTCGCAGG AGGTAGAGAG CCATTACGTT 
GAAGGTCTGT GTAAACAGTT TTATGAAGAC ATGGTGGACA TCATCCAGGC GACGGTTCAG 
CAGAACTTTG ACACAGAGAC AGATCCTCTC TATGATGAAA TTATACAACA TTTGTCGCTG 
TGCAAGACGT TTTCATCTTT TTATGTGTAC AAGAGCGAAG CCTTGGATAT AGTCCAGGAG 
TACCTGTACC CCTCGAAAGG TGGTCGAATA ACTCCCCAAG TGGTTTACGG AGGACCCTGC 
ACTGGAAAGA CCTTGTTACT TGCTGAAGTG GCAAAACAGG CCTATTCATG GATACAGAAA 
GAGATGGGAC CAGAAACAGA CCCAGTGGTC ATTGTCAGAT TCATCGGTTC CACTGACTTC 
TCTACTGACC TCCGCACCCT GCTGCAGAGC ATCTGTGAGC AGATTGCCAT CAACTATCGG 
TGCCTGATCC ATTTCTTACC AAACAAGATC CAGGAGATGA GAGAACTGCT GGTGAATTTG 
CTGGGGGAGT CGTCATTCCA CCGACCTCTT GTCATTATTC TAGATGCCTT GGAGCAGTTG 
TCAGACGCAG ACGAGGCTCG AAAACTGTGG TGGCTCCCGG TCCACCTGCC ACGTACAGTT 
CGAATAGTAG TGTCCACCCT GCCCAACAAG CACGGAATAC TGCAGAAGCT GCGTTGCCTG 
ATACATGATG AGGAAGCCTA TGTTGAACTA ATGCAAAGAG ATCGCAAAGC TTGCAGTCAG 
ACCCTTAAAC AGCAGCTTCT GGGTGTCAAA AGGAAGGTGA CGTCTGGCCA ACAGATATAT 
GTTAATGAGG CAATGGCAAA ATGTACACTT CCTATGTTTG TTAACCTCAT CTACAGAGAG 
GTTGTTCACT GGAGATCGCA CAAGGATGTG GATGACAAAT CGTTGTGTTC CACAGTACAC 
GAAAGTATTG AGCAGCTTTT CTACTCAGTT GAAAATAAGC TTGGCTCACG CTTTGTCTTT 
CGGGCACTGG GCTACATCAC AATGGCACGG GCAGGCCTGA CTGAGCTGGA GCTAGAGGAT 
ATCTTATCTT TAGATAACAG TGTCCTAGGG GACATCATGG TGAGCTCTAA TCTCAAAAGC 
CCACTGCGTA TTTCCTATGA TTTCATAGCT CGGCTAAAAG AAGAACTTGA AGGTTATTTA 
GTGGAGCGTC AGGTCCATAA TGTAACACTA ATGGTGTGGG CAAATCGGCA TCTACATCTC 
ATAGCACAGA AGCTGTACCT CAGTAACGAA GAAGACGTGC ATCAAATGCA TAGCCTTTTG 
GCTGAGTACT TTCTTGGGGC ATGGGCAGGT GGCAGAAAGA AAATATTCCA CTGCGACAAT 
AATCACTTTG CCTCATTGAA TATCTCTCAG CATCGAAATC CTCACAACCA ACAGAGCCTT 
CATGGCCATG ACAAAGCTTC TGCTGACAAG CACTCATATG ACAGACAGAC ACCTGAGCAA 
CCCTGGGTGT TCCAATGTAA TCTGCTGGAG CCGGACATCT TCTTTGTCAA CCACCGTAAG 
ATGACAGAGC TCACATACCA CTTGACAAGG AGCGGCCGCA CAGATGATCT CATGTATGGT 
GTCATCATGA ACTTCAGTTG GCTCTACACT ATGATAAAGA TAGGGCAATT TGACAAGGCC 
CTGTCTGACA TTGACCTAGC CTACAGCTAT ACGCAAGAAA AGGAGCTAAA GTTTCTTGCA 
ACTACACTCC GCAGTATAAA GCTTAAAGTG ACAAAGAATC CAGCATCACT GTCTGCTGAA 
CTACAGCAAA GGCTCCTTCC AGTGGTCACA TCCCTTCCTA AACTCAGACA CCTCCTGCTT 
GAGTGTGACA AGGACGGCCC TAAATACTGC TCCATCGTGC CACTTCACTC CTCTATGGAT 
GTGACCTACA GTCCAGAGAG ATTGCCGCTC AGTTCTAGCT ACATGCAGAT AGTTGAGACT 
CTGCCTACAT TAGCTCCAAA CATAGTCATT GTGGCTCTTG AAGATGGGTC CGTCAGCACT 
TGGGATGTTG AAAGCAGACA GCTGCTAAGA CAGATAGACA CAGCAAGGTC AGTTGTTCTT 
GGAATTAGAC TAACCACTGA TGAGAAATAT CTGGTAGTGG CCACTACAAA AAATACACTG 
CTCATTTATG ACAACCACAA GTCTTGTTTG TTGTCTGAGG TAGAGGTCAA AGGGTCCAAA 
CACTGTGGGA TCACTGGTGG GGTGGCATTC ATTAATGGAT TTACCTTGTC CAGCCACCAT 
GCCTTAGCTT GGCTTGAGGC AAGCAAAGAT GTCAGTGTGA TTGATTTGCT GTACGGCTGG 
CCATTGTATC AGTTCCATTG CTGGTATGAG GTAACCTGTG TCCAGTGTTC CCCAGATGGA 
ATGTATGCTT TCTGTGGACA GTACCTCAAC ACAACCACAA TATTCCACCT TGGTAGTGGG 
GATAAGCTTG CCACAATGAC TTCTGAATTC TCAGGTGGTT TCGTGAAGTC CATTCTGATT 
CTAGACACAA TCAATCAAAT GGTGATGATT GACAATGAGG GCAGTCTCTC CGTATGGAGC 
ACCAAAGATA TCACAAACCC CAAGCTAATG GAGGATTATG ACTGCAGAGG AGATGATAGT 
GAGGTGGTGG GCATAGAGCT CTCAGAGGAT CAGCGATCCA TTCTAATCTG CAAAGCAAGA 
AGCATTGAGG TTCTGGACAC CAAAATTTGG AAGATGGCTG AAAAGTTTAA GGCCAAGCGC 
ACTGAGAAGT TTGTGGCAGC TGTTCTCTCC AAGAATAGCC AAAGTATAAT AGCCTCCATG 
GAGAACACAT CATCCATATT TGTGTGGAGG AGAGACAGTG GACAGTGCAT GGCCAGCTTG 
ATAGAGATAT CGGGAGCCAT TGTAAAGCTA ATCAAATCAA CACACCATAA CTTGCTTCTG 
TCTGTGGCCA GCAGTGGGGT GCTTTCCGTG TGGGATATCG ACATTATAAC AGCCATGTCG 
AACATTGATA AAACTGGAAA ACCTATCCAA ATTCTGCAAC TCTCTGGCAG AGAGGATTTT 
GTATACACCA TGGATGGGTC AGAAGTCATC CATAAATGGA ACTACAGCAC AGGTTTCATT 
GAAATGGTGT TCAAACATGA AGGACTTGTT GAGAACTGTG TCCTGACAAC ATCAGGTGAC 
CTAATGGTGA CATCTGACGA TAAAAGTAGT CAGTACATCT GGCATACGAC CACAGGTGAG 
AATATATTCC GTATCAATGG CCAGAAAATC TCACAGCTTC TCATCACGCA CAATGACCAA 
TTTGTTGTGT CACTTTGTGA GCAGAATGCC TCTCGTGTCT GGAGGCTTGC CACTGGTCAC 
AAGGTGTGCA ACATTCTGGT AACACTTCAA CATGCCCTCA TTACTACAGC AAACACATTC 
CTTGTGGGTG CAAACAAGAA TAAGCTGCTG GCTGTTAGCT TATGGTCAGG GAGTGTTTCC 
AAAAAGTTTA TCTGTGATGA TGGGATCACC ATTGTTAACT TCAAGTTAAT CCCTGACAGT 
CCAGATTTTG TTGTCTTCAT CACCTCCACT GAAACGGTAT TCATCTGGAG TGTTGCCGAT 
GAGTCAGTAT GTAGACGGGT GCAACTCCCC AACAACTTCT TAAAGAACCT GGAAGATTTC 
CAAATTTCAC CGAATGGGAA ATTGGGCATT GTCTCAAAAG GTGATGAGAA CATCAATGTT 
CTAGACCTAC ACAGTGGTAA GCTGCGTCTG GTGCATGCTG CTGGCATAAT ATGGCGGCAA 
AAGCTTTCAA GAGATGGACG CTACTTAGTA TACATCTGTT TCCGGAACTG CGATGAAGAT 
GATGATGCAG GTGTGGTTTC AAGCCTGATT GTCATGAGAC TGGCAGATGG TAAGAGTATC 
GGGACCTGTT CCCTTTACAA AACGCCAACC TATCTGTGCC TATCACAGCG AGCACTTAAC 
ATCATTGTCG GATTTGAGGA TGGCAGCATT GGCACTTATA CAGTGGTTGA TCGTGTAGAT 
GCTGCTCTGA AGATCAAGAT CGCCACCTCA AACAGTCGCC AAATTGTTAA CAACGCCTCA 
CAGAAAGTAC GACCCAAGTG TAGCAACAAT GCCTTCAAGA CCATTGCTGA CTGTGTGTGG 
AGAGAATCCA CTGAGGTCTT CTCTCGAGAC AGTCCTATCA ACGTGTCTGA TGGTGGGGAG 
CCAGAGACCA CCACACCCAC AAAGAAAACT GAACTGCTGC AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172417.1
CDS	145..4929
Translation	MILDAAVEAGLDTRILEEWYCRDENSVPPAYYLKPKAEMLKNYQNSMESNTVAKSKNDKAWRAVSEEIKRIFRTSVLQLQEKGTMKSGQAKKFLCSALEDELDFALGKQTPAFLKKCVCYIRKISNFDRFAKLPEMARYMDIVVSDDRVMRNQEAYERLLKVRDEFIPVVVAASNLRVYSSVTHCDMKLGYSQEVESHYVEGLCKQFYEDMVDIIQATVQQNFDTETDPLYDEIIQHLSLCKTFSSFYVYKSEALDIVQEYLYPSKGGRITPQVVYGGPCTGKTLLLAEVAKQAYSWIQKEMGPETDPVVIVRFIGSTDFSTDLRTLLQSICEQIAINYRCLIHFLPNKIQEMRELLVNLLGESSFHRPLVIILDALEQLSDADEARKLWWLPVHLPRTVRIVVSTLPNKHGILQKLRCLIHDEEAYVELMQRDRKACSQTLKQQLLGVKRKVTSGQQIYVNEAMAKCTLPMFVNLIYREVVHWRSHKDVDDKSLCSTVHESIEQLFYSVENKLGSRFVFRALGYITMARAGLTELELEDILSLDNSVLGDIMVSSNLKSPLRISYDFIARLKEELEGYLVERQVHNVTLMVWANRHLHLIAQKLYLSNEEDVHQMHSLLAEYFLGAWAGGRKKIFHCDNNHFASLNISQHRNPHNQQSLHGHDKASADKHSYDRQTPEQPWVFQCNLLEPDIFFVNHRKMTELTYHLTRSGRTDDLMYGVIMNFSWLYTMIKIGQFDKALSDIDLAYSYTQEKELKFLATTLRSIKLKVTKNPASLSAELQQRLLPVVTSLPKLRHLLLECDKDGPKYCSIVPLHSSMDVTYSPERLPLSSSYMQIVETLPTLAPNIVIVALEDGSVSTWDVESRQLLRQIDTARSVVLGIRLTTDEKYLVVATTKNTLLIYDNHKSCLLSEVEVKGSKHCGITGGVAFINGFTLSSHHALAWLEASKDVSVIDLLYGWPLYQFHCWYEVTCVQCSPDGMYAFCGQYLNTTTIFHLGSGDKLATMTSEFSGGFVKSILILDTINQMVMIDNEGSLSVWSTKDITNPKLMEDYDCRGDDSEVVGIELSEDQRSILICKARSIEVLDTKIWKMAEKFKAKRTEKFVAAVLSKNSQSIIASMENTSSIFVWRRDSGQCMASLIEISGAIVKLIKSTHHNLLLSVASSGVLSVWDIDIITAMSNIDKTGKPIQILQLSGREDFVYTMDGSEVIHKWNYSTGFIEMVFKHEGLVENCVLTTSGDLMVTSDDKSSQYIWHTTTGENIFRINGQKISQLLITHNDQFVVSLCEQNASRVWRLATGHKVCNILVTLQHALITTANTFLVGANKNKLLAVSLWSGSVSKKFICDDGITIVNFKLIPDSPDFVVFITSTETVFIWSVADESVCRRVQLPNNFLKNLEDFQISPNGKLGIVSKGDENINVLDLHSGKLRLVHAAGIIWRQKLSRDGRYLVYICFRNCDEDDDAGVVSSLIVMRLADGKSIGTCSLYKTPTYLCLSQRALNIIVGFEDGSIGTYTVVDRVDAALKIKIATSNSRQIVNNASQKVRPKCSNNAFKTIADCVWRESTEVFSRDSPINVSDGGEPETTTPTKKTELLQ

Target ORF information:

RefSeq Version	XM_005172360.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NACHT and WD repeat domain containing 2 (nwd2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172360.2

ORF Insert Sequence:

ATGATTTTGG ATGCGGCAGT GGAGGCGGGA CTAGACACAC GGATCTTGGA GGAGTGGTAC 
TGTCGAGATG AGAACTCAGT CCCACCTGCC TACTACCTAA AACCAAAAGC TGAGATGCTG 
AAAAACTATC AGAATTCTAT GGAGTCAAAC ACCGTAGCAA AATCAAAGAA CGACAAGGCA 
TGGAGGGCAG TCTCAGAGGA AATCAAGAGG ATCTTCAGAA CCTCTGTTTT ACAGCTGCAG 
GAGAAGGGGA CCATGAAGAG TGGTCAAGCC AAGAAATTTC TCTGCTCAGC ATTAGAGGAT 
GAACTGGACT TCGCATTGGG CAAGCAGACC CCAGCCTTTC TCAAAAAATG TGTTTGTTAC 
ATTCGGAAGA TATCTAACTT TGATCGTTTC GCTAAACTCC CCGAAATGGC ACGTTACATG 
GACATTGTGG TGAGCGATGA CCGTGTCATG AGAAACCAGG AAGCCTACGA GCGTCTGCTA 
AAGGTCAGAG ACGAGTTCAT TCCGGTGGTG GTGGCAGCAT CCAATCTCCG AGTCTACTCA 
TCGGTCACTC ACTGTGACAT GAAACTTGGC TACTCGCAGG AGGTAGAGAG CCATTACGTT 
GAAGGTCTGT GTAAACAGTT TTATGAAGAC ATGGTGGACA TCATCCAGGC GACGGTTCAG 
CAGAACTTTG ACACAGAGAC AGATCCTCTC TATGATGAAA TTATACAACA TTTGTCGCTG 
TGCAAGACGT TTTCATCTTT TTATGTGTAC AAGAGCGAAG CCTTGGATAT AGTCCAGGAG 
TACCTGTACC CCTCGAAAGG TGGTCGAATA ACTCCCCAAG TGGTTTACGG AGGACCCTGC 
ACTGGAAAGA CCTTGTTACT TGCTGAAGTG GCAAAACAGG CCTATTCATG GATACAGAAA 
GAGATGGGAC CAGAAACAGA CCCAGTGGTC ATTGTCAGAT TCATCGGTTC CACTGACTTC 
TCTACTGACC TCCGCACCCT GCTGCAGAGC ATCTGTGAGC AGATTGCCAT CAACTATCGG 
TGCCTGATCC ATTTCTTACC AAACAAGATC CAGGAGATGA GAGAACTGCT GGTGAATTTG 
CTGGGGGAGT CGTCATTCCA CCGACCTCTT GTCATTATTC TAGATGCCTT GGAGCAGTTG 
TCAGACGCAG ACGAGGCTCG AAAACTGTGG TGGCTCCCGG TCCACCTGCC ACGTACAGTT 
CGAATAGTAG TGTCCACCCT GCCCAACAAG CACGGAATAC TGCAGAAGCT GCGTTGCCTG 
ATACATGATG AGGAAGCCTA TGTTGAACTA ATGCAAAGAG ATCGCAAAGC TTGCAGTCAG 
ACCCTTAAAC AGCAGCTTCT GGGTGTCAAA AGGAAGGTGA CGTCTGGCCA ACAGATATAT 
GTTAATGAGG CAATGGCAAA ATGTACACTT CCTATGTTTG TTAACCTCAT CTACAGAGAG 
GTTGTTCACT GGAGATCGCA CAAGGATGTG GATGACAAAT CGTTGTGTTC CACAGTACAC 
GAAAGTATTG AGCAGCTTTT CTACTCAGTT GAAAATAAGC TTGGCTCACG CTTTGTCTTT 
CGGGCACTGG GCTACATCAC AATGGCACGG GCAGGCCTGA CTGAGCTGGA GCTAGAGGAT 
ATCTTATCTT TAGATAACAG TGTCCTAGGG GACATCATGG TGAGCTCTAA TCTCAAAAGC 
CCACTGCGTA TTTCCTATGA TTTCATAGCT CGGCTAAAAG AAGAACTTGA AGGTTATTTA 
GTGGAGCGTC AGGTCCATAA TGTAACACTA ATGGTGTGGG CAAATCGGCA TCTACATCTC 
ATAGCACAGA AGCTGTACCT CAGTAACGAA GAAGACGTGC ATCAAATGCA TAGCCTTTTG 
GCTGAGTACT TTCTTGGGGC ATGGGCAGGT GGCAGAAAGA AAATATTCCA CTGCGACAAT 
AATCACTTTG CCTCATTGAA TATCTCTCAG CATCGAAATC CTCACAACCA ACAGAGCCTT 
CATGGCCATG ACAAAGCTTC TGCTGACAAG CACTCATATG ACAGACAGAC ACCTGAGCAA 
CCCTGGGTGT TCCAATGTAA TCTGCTGGAG CCGGACATCT TCTTTGTCAA CCACCGTAAG 
ATGACAGAGC TCACATACCA CTTGACAAGG AGCGGCCGCA CAGATGATCT CATGTATGGT 
GTCATCATGA ACTTCAGTTG GCTCTACACT ATGATAAAGA TAGGGCAATT TGACAAGGCC 
CTGTCTGACA TTGACCTAGC CTACAGCTAT ACGCAAGAAA AGGAGCTAAA GTTTCTTGCA 
ACTACACTCC GCAGTATAAA GCTTAAAGTG ACAAAGAATC CAGCATCACT GTCTGCTGAA 
CTACAGCAAA GGCTCCTTCC AGTGGTCACA TCCCTTCCTA AACTCAGACA CCTCCTGCTT 
GAGTGTGACA AGGACGGCCC TAAATACTGC TCCATCGTGC CACTTCACTC CTCTATGGAT 
GTGACCTACA GTCCAGAGAG ATTGCCGCTC AGTTCTAGCT ACATGCAGAT AGTTGAGACT 
CTGCCTACAT TAGCTCCAAA CATAGTCATT GTGGCTCTTG AAGATGGGTC CGTCAGCACT 
TGGGATGTTG AAAGCAGACA GCTGCTAAGA CAGATAGACA CAGCAAGGTC AGTTGTTCTT 
GGAATTAGAC TAACCACTGA TGAGAAATAT CTGGTAGTGG CCACTACAAA AAATACACTG 
CTCATTTATG ACAACCACAA GTCTTGTTTG TTGTCTGAGG TAGAGGTCAA AGGGTCCAAA 
CACTGTGGGA TCACTGGTGG GGTGGCATTC ATTAATGGAT TTACCTTGTC CAGCCACCAT 
GCCTTAGCTT GGCTTGAGGC AAGCAAAGAT GTCAGTGTGA TTGATTTGCT GTACGGCTGG 
CCATTGTATC AGTTCCATTG CTGGTATGAG GTAACCTGTG TCCAGTGTTC CCCAGATGGA 
ATGTATGCTT TCTGTGGACA GTACCTCAAC ACAACCACAA TATTCCACCT TGGTAGTGGG 
GATAAGCTTG CCACAATGAC TTCTGAATTC TCAGGTGGTT TCGTGAAGTC CATTCTGATT 
CTAGACACAA TCAATCAAAT GGTGATGATT GACAATGAGG GCAGTCTCTC CGTATGGAGC 
ACCAAAGATA TCACAAACCC CAAGCTAATG GAGGATTATG ACTGCAGAGG AGATGATAGT 
GAGGTGGTGG GCATAGAGCT CTCAGAGGAT CAGCGATCCA TTCTAATCTG CAAAGCAAGA 
AGCATTGAGG TTCTGGACAC CAAAATTTGG AAGATGGCTG AAAAGTTTAA GGCCAAGCGC 
ACTGAGAAGT TTGTGGCAGC TGTTCTCTCC AAGAATAGCC AAAGTATAAT AGCCTCCATG 
GAGAACACAT CATCCATATT TGTGTGGAGG AGAGACAGTG GACAGTGCAT GGCCAGCTTG 
ATAGAGATAT CGGGAGCCAT TGTAAAGCTA ATCAAATCAA CACACCATAA CTTGCTTCTG 
TCTGTGGCCA GCAGTGGGGT GCTTTCCGTG TGGGATATCG ACATTATAAC AGCCATGTCG 
AACATTGATA AAACTGGAAA ACCTATCCAA ATTCTGCAAC TCTCTGGCAG AGAGGATTTT 
GTATACACCA TGGATGGGTC AGAAGTCATC CATAAATGGA ACTACAGCAC AGGTTTCATT 
GAAATGGTGT TCAAACATGA AGGACTTGTT GAGAACTGTG TCCTGACAAC ATCAGGTGAC 
CTAATGGTGA CATCTGACGA TAAAAGTAGT CAGTACATCT GGCATACGAC CACAGGTGAG 
AATATATTCC GTATCAATGG CCAGAAAATC TCACAGCTTC TCATCACGCA CAATGACCAA 
TTTGTTGTGT CACTTTGTGA GCAGAATGCC TCTCGTGTCT GGAGGCTTGC CACTGGTCAC 
AAGGTGTGCA ACATTCTGGT AACACTTCAA CATGCCCTCA TTACTACAGC AAACACATTC 
CTTGTGGGTG CAAACAAGAA TAAGCTGCTG GCTGTTAGCT TATGGTCAGG GAGTGTTTCC 
AAAAAGTTTA TCTGTGATGA TGGGATCACC ATTGTTAACT TCAAGTTAAT CCCTGACAGT 
CCAGATTTTG TTGTCTTCAT CACCTCCACT GAAACGGTAT TCATCTGGAG TGTTGCCGAT 
GAGTCAGTAT GTAGACGGGT GCAACTCCCC AACAACTTCT TAAAGAACCT GGAAGATTTC 
CAAATTTCAC CGAATGGGAA ATTGGGCATT GTCTCAAAAG GTGATGAGAA CATCAATGTT 
CTAGACCTAC ACAGTGGTAA GCTGCGTCTG GTGCATGCTG CTGGCATAAT ATGGCGGCAA 
AAGCTTTCAA GAGATGGACG CTACTTAGTA TACATCTGTT TCCGGAACTG CGATGAAGAT 
GATGATGCAG GTGTGGTTTC AAGCCTGATT GTCATGAGAC TGGCAGATGG TAAGAGTATC 
GGGACCTGTT CCCTTTACAA AACGCCAACC TATCTGTGCC TATCACAGCG AGCACTTAAC 
ATCATTGTCG GATTTGAGGA TGGCAGCATT GGCACTTATA CAGTGGTTGA TCGTGTAGAT 
GCTGCTCTGA AGATCAAGAT CGCCACCTCA AACAGTCGCC AAATTGTTAA CAACGCCTCA 
CAGAAAGTAC GACCCAAGTG TAGCAACAAT GCCTTCAAGA CCATTGCTGA CTGTGTGTGG 
AGAGAATCCA CTGAGGTCTT CTCTCGAGAC AGTCCTATCA ACGTGTCTGA TGGTGGGGAG 
CCAGAGACCA CCACACCCAC AAAGAAAACT GAACTGCTGC AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nwd2 ( XM_005172360.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172417.1 Danio rerio NACHT and WD repeat domain containing 2 (nwd2), transcript variant X3, mRNA.

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CloneID	ODa13556
Clone ID Related Accession (Same CDS sequence)	XM_005172360.2 , XM_005172360.3
Accession Version	XM_005172360.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4785bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NACHT and WD repeat domain-containing protein 2 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172360.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATTTTGG ATGCGGCAGT GGAGGCGGGA CTAGACACAC GGATCTTGGA GGAGTGGTAC 
TGTCGAGATG AGAACTCAGT CCCACCTGCC TACTACCTAA AACCAAAAGC TGAGATGCTG 
AAAAACTATC AGAATTCTAT GGAGTCAAAC ACCGTAGCAA AATCAAAGAA CGACAAGGCA 
TGGAGGGCAG TCTCAGAGGA AATCAAGAGG ATCTTCAGAA CCTCTGTTTT ACAGCTGCAG 
GAGAAGGGGA CCATGAAGAG TGGTCAAGCC AAGAAATTTC TCTGCTCAGC ATTAGAGGAT 
GAACTGGACT TCGCATTGGG CAAGCAGACC CCAGCCTTTC TCAAAAAATG TGTTTGTTAC 
ATTCGGAAGA TATCTAACTT TGATCGTTTC GCTAAACTCC CCGAAATGGC ACGTTACATG 
GACATTGTGG TGAGCGATGA CCGTGTCATG AGAAACCAGG AAGCCTACGA GCGTCTGCTA 
AAGGTCAGAG ACGAGTTCAT TCCGGTGGTG GTGGCAGCAT CCAATCTCCG AGTCTACTCA 
TCGGTCACTC ACTGTGACAT GAAACTTGGC TACTCGCAGG AGGTAGAGAG CCATTACGTT 
GAAGGTCTGT GTAAACAGTT TTATGAAGAC ATGGTGGACA TCATCCAGGC GACGGTTCAG 
CAGAACTTTG ACACAGAGAC AGATCCTCTC TATGATGAAA TTATACAACA TTTGTCGCTG 
TGCAAGACGT TTTCATCTTT TTATGTGTAC AAGAGCGAAG CCTTGGATAT AGTCCAGGAG 
TACCTGTACC CCTCGAAAGG TGGTCGAATA ACTCCCCAAG TGGTTTACGG AGGACCCTGC 
ACTGGAAAGA CCTTGTTACT TGCTGAAGTG GCAAAACAGG CCTATTCATG GATACAGAAA 
GAGATGGGAC CAGAAACAGA CCCAGTGGTC ATTGTCAGAT TCATCGGTTC CACTGACTTC 
TCTACTGACC TCCGCACCCT GCTGCAGAGC ATCTGTGAGC AGATTGCCAT CAACTATCGG 
TGCCTGATCC ATTTCTTACC AAACAAGATC CAGGAGATGA GAGAACTGCT GGTGAATTTG 
CTGGGGGAGT CGTCATTCCA CCGACCTCTT GTCATTATTC TAGATGCCTT GGAGCAGTTG 
TCAGACGCAG ACGAGGCTCG AAAACTGTGG TGGCTCCCGG TCCACCTGCC ACGTACAGTT 
CGAATAGTAG TGTCCACCCT GCCCAACAAG CACGGAATAC TGCAGAAGCT GCGTTGCCTG 
ATACATGATG AGGAAGCCTA TGTTGAACTA ATGCAAAGAG ATCGCAAAGC TTGCAGTCAG 
ACCCTTAAAC AGCAGCTTCT GGGTGTCAAA AGGAAGGTGA CGTCTGGCCA ACAGATATAT 
GTTAATGAGG CAATGGCAAA ATGTACACTT CCTATGTTTG TTAACCTCAT CTACAGAGAG 
GTTGTTCACT GGAGATCGCA CAAGGATGTG GATGACAAAT CGTTGTGTTC CACAGTACAC 
GAAAGTATTG AGCAGCTTTT CTACTCAGTT GAAAATAAGC TTGGCTCACG CTTTGTCTTT 
CGGGCACTGG GCTACATCAC AATGGCACGG GCAGGCCTGA CTGAGCTGGA GCTAGAGGAT 
ATCTTATCTT TAGATAACAG TGTCCTAGGG GACATCATGG TGAGCTCTAA TCTCAAAAGC 
CCACTGCGTA TTTCCTATGA TTTCATAGCT CGGCTAAAAG AAGAACTTGA AGGTTATTTA 
GTGGAGCGTC AGGTCCATAA TGTAACACTA ATGGTGTGGG CAAATCGGCA TCTACATCTC 
ATAGCACAGA AGCTGTACCT CAGTAACGAA GAAGACGTGC ATCAAATGCA TAGCCTTTTG 
GCTGAGTACT TTCTTGGGGC ATGGGCAGGT GGCAGAAAGA AAATATTCCA CTGCGACAAT 
AATCACTTTG CCTCATTGAA TATCTCTCAG CATCGAAATC CTCACAACCA ACAGAGCCTT 
CATGGCCATG ACAAAGCTTC TGCTGACAAG CACTCATATG ACAGACAGAC ACCTGAGCAA 
CCCTGGGTGT TCCAATGTAA TCTGCTGGAG CCGGACATCT TCTTTGTCAA CCACCGTAAG 
ATGACAGAGC TCACATACCA CTTGACAAGG AGCGGCCGCA CAGATGATCT CATGTATGGT 
GTCATCATGA ACTTCAGTTG GCTCTACACT ATGATAAAGA TAGGGCAATT TGACAAGGCC 
CTGTCTGACA TTGACCTAGC CTACAGCTAT ACGCAAGAAA AGGAGCTAAA GTTTCTTGCA 
ACTACACTCC GCAGTATAAA GCTTAAAGTG ACAAAGAATC CAGCATCACT GTCTGCTGAA 
CTACAGCAAA GGCTCCTTCC AGTGGTCACA TCCCTTCCTA AACTCAGACA CCTCCTGCTT 
GAGTGTGACA AGGACGGCCC TAAATACTGC TCCATCGTGC CACTTCACTC CTCTATGGAT 
GTGACCTACA GTCCAGAGAG ATTGCCGCTC AGTTCTAGCT ACATGCAGAT AGTTGAGACT 
CTGCCTACAT TAGCTCCAAA CATAGTCATT GTGGCTCTTG AAGATGGGTC CGTCAGCACT 
TGGGATGTTG AAAGCAGACA GCTGCTAAGA CAGATAGACA CAGCAAGGTC AGTTGTTCTT 
GGAATTAGAC TAACCACTGA TGAGAAATAT CTGGTAGTGG CCACTACAAA AAATACACTG 
CTCATTTATG ACAACCACAA GTCTTGTTTG TTGTCTGAGG TAGAGGTCAA AGGGTCCAAA 
CACTGTGGGA TCACTGGTGG GGTGGCATTC ATTAATGGAT TTACCTTGTC CAGCCACCAT 
GCCTTAGCTT GGCTTGAGGC AAGCAAAGAT GTCAGTGTGA TTGATTTGCT GTACGGCTGG 
CCATTGTATC AGTTCCATTG CTGGTATGAG GTAACCTGTG TCCAGTGTTC CCCAGATGGA 
ATGTATGCTT TCTGTGGACA GTACCTCAAC ACAACCACAA TATTCCACCT TGGTAGTGGG 
GATAAGCTTG CCACAATGAC TTCTGAATTC TCAGGTGGTT TCGTGAAGTC CATTCTGATT 
CTAGACACAA TCAATCAAAT GGTGATGATT GACAATGAGG GCAGTCTCTC CGTATGGAGC 
ACCAAAGATA TCACAAACCC CAAGCTAATG GAGGATTATG ACTGCAGAGG AGATGATAGT 
GAGGTGGTGG GCATAGAGCT CTCAGAGGAT CAGCGATCCA TTCTAATCTG CAAAGCAAGA 
AGCATTGAGG TTCTGGACAC CAAAATTTGG AAGATGGCTG AAAAGTTTAA GGCCAAGCGC 
ACTGAGAAGT TTGTGGCAGC TGTTCTCTCC AAGAATAGCC AAAGTATAAT AGCCTCCATG 
GAGAACACAT CATCCATATT TGTGTGGAGG AGAGACAGTG GACAGTGCAT GGCCAGCTTG 
ATAGAGATAT CGGGAGCCAT TGTAAAGCTA ATCAAATCAA CACACCATAA CTTGCTTCTG 
TCTGTGGCCA GCAGTGGGGT GCTTTCCGTG TGGGATATCG ACATTATAAC AGCCATGTCG 
AACATTGATA AAACTGGAAA ACCTATCCAA ATTCTGCAAC TCTCTGGCAG AGAGGATTTT 
GTATACACCA TGGATGGGTC AGAAGTCATC CATAAATGGA ACTACAGCAC AGGTTTCATT 
GAAATGGTGT TCAAACATGA AGGACTTGTT GAGAACTGTG TCCTGACAAC ATCAGGTGAC 
CTAATGGTGA CATCTGACGA TAAAAGTAGT CAGTACATCT GGCATACGAC CACAGGTGAG 
AATATATTCC GTATCAATGG CCAGAAAATC TCACAGCTTC TCATCACGCA CAATGACCAA 
TTTGTTGTGT CACTTTGTGA GCAGAATGCC TCTCGTGTCT GGAGGCTTGC CACTGGTCAC 
AAGGTGTGCA ACATTCTGGT AACACTTCAA CATGCCCTCA TTACTACAGC AAACACATTC 
CTTGTGGGTG CAAACAAGAA TAAGCTGCTG GCTGTTAGCT TATGGTCAGG GAGTGTTTCC 
AAAAAGTTTA TCTGTGATGA TGGGATCACC ATTGTTAACT TCAAGTTAAT CCCTGACAGT 
CCAGATTTTG TTGTCTTCAT CACCTCCACT GAAACGGTAT TCATCTGGAG TGTTGCCGAT 
GAGTCAGTAT GTAGACGGGT GCAACTCCCC AACAACTTCT TAAAGAACCT GGAAGATTTC 
CAAATTTCAC CGAATGGGAA ATTGGGCATT GTCTCAAAAG GTGATGAGAA CATCAATGTT 
CTAGACCTAC ACAGTGGTAA GCTGCGTCTG GTGCATGCTG CTGGCATAAT ATGGCGGCAA 
AAGCTTTCAA GAGATGGACG CTACTTAGTA TACATCTGTT TCCGGAACTG CGATGAAGAT 
GATGATGCAG GTGTGGTTTC AAGCCTGATT GTCATGAGAC TGGCAGATGG TAAGAGTATC 
GGGACCTGTT CCCTTTACAA AACGCCAACC TATCTGTGCC TATCACAGCG AGCACTTAAC 
ATCATTGTCG GATTTGAGGA TGGCAGCATT GGCACTTATA CAGTGGTTGA TCGTGTAGAT 
GCTGCTCTGA AGATCAAGAT CGCCACCTCA AACAGTCGCC AAATTGTTAA CAACGCCTCA 
CAGAAAGTAC GACCCAAGTG TAGCAACAAT GCCTTCAAGA CCATTGCTGA CTGTGTGTGG 
AGAGAATCCA CTGAGGTCTT CTCTCGAGAC AGTCCTATCA ACGTGTCTGA TGGTGGGGAG 
CCAGAGACCA CCACACCCAC AAAGAAAACT GAACTGCTGC AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172417.1
CDS	194..4978
Translation	MILDAAVEAGLDTRILEEWYCRDENSVPPAYYLKPKAEMLKNYQNSMESNTVAKSKNDKAWRAVSEEIKRIFRTSVLQLQEKGTMKSGQAKKFLCSALEDELDFALGKQTPAFLKKCVCYIRKISNFDRFAKLPEMARYMDIVVSDDRVMRNQEAYERLLKVRDEFIPVVVAASNLRVYSSVTHCDMKLGYSQEVESHYVEGLCKQFYEDMVDIIQATVQQNFDTETDPLYDEIIQHLSLCKTFSSFYVYKSEALDIVQEYLYPSKGGRITPQVVYGGPCTGKTLLLAEVAKQAYSWIQKEMGPETDPVVIVRFIGSTDFSTDLRTLLQSICEQIAINYRCLIHFLPNKIQEMRELLVNLLGESSFHRPLVIILDALEQLSDADEARKLWWLPVHLPRTVRIVVSTLPNKHGILQKLRCLIHDEEAYVELMQRDRKACSQTLKQQLLGVKRKVTSGQQIYVNEAMAKCTLPMFVNLIYREVVHWRSHKDVDDKSLCSTVHESIEQLFYSVENKLGSRFVFRALGYITMARAGLTELELEDILSLDNSVLGDIMVSSNLKSPLRISYDFIARLKEELEGYLVERQVHNVTLMVWANRHLHLIAQKLYLSNEEDVHQMHSLLAEYFLGAWAGGRKKIFHCDNNHFASLNISQHRNPHNQQSLHGHDKASADKHSYDRQTPEQPWVFQCNLLEPDIFFVNHRKMTELTYHLTRSGRTDDLMYGVIMNFSWLYTMIKIGQFDKALSDIDLAYSYTQEKELKFLATTLRSIKLKVTKNPASLSAELQQRLLPVVTSLPKLRHLLLECDKDGPKYCSIVPLHSSMDVTYSPERLPLSSSYMQIVETLPTLAPNIVIVALEDGSVSTWDVESRQLLRQIDTARSVVLGIRLTTDEKYLVVATTKNTLLIYDNHKSCLLSEVEVKGSKHCGITGGVAFINGFTLSSHHALAWLEASKDVSVIDLLYGWPLYQFHCWYEVTCVQCSPDGMYAFCGQYLNTTTIFHLGSGDKLATMTSEFSGGFVKSILILDTINQMVMIDNEGSLSVWSTKDITNPKLMEDYDCRGDDSEVVGIELSEDQRSILICKARSIEVLDTKIWKMAEKFKAKRTEKFVAAVLSKNSQSIIASMENTSSIFVWRRDSGQCMASLIEISGAIVKLIKSTHHNLLLSVASSGVLSVWDIDIITAMSNIDKTGKPIQILQLSGREDFVYTMDGSEVIHKWNYSTGFIEMVFKHEGLVENCVLTTSGDLMVTSDDKSSQYIWHTTTGENIFRINGQKISQLLITHNDQFVVSLCEQNASRVWRLATGHKVCNILVTLQHALITTANTFLVGANKNKLLAVSLWSGSVSKKFICDDGITIVNFKLIPDSPDFVVFITSTETVFIWSVADESVCRRVQLPNNFLKNLEDFQISPNGKLGIVSKGDENINVLDLHSGKLRLVHAAGIIWRQKLSRDGRYLVYICFRNCDEDDDAGVVSSLIVMRLADGKSIGTCSLYKTPTYLCLSQRALNIIVGFEDGSIGTYTVVDRVDAALKIKIATSNSRQIVNNASQKVRPKCSNNAFKTIADCVWRESTEVFSRDSPINVSDGGEPETTTPTKKTELLQ

Target ORF information:

RefSeq Version	XM_005172360.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NACHT and WD repeat domain containing 2 (nwd2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172360.3

ORF Insert Sequence:

ATGATTTTGG ATGCGGCAGT GGAGGCGGGA CTAGACACAC GGATCTTGGA GGAGTGGTAC 
TGTCGAGATG AGAACTCAGT CCCACCTGCC TACTACCTAA AACCAAAAGC TGAGATGCTG 
AAAAACTATC AGAATTCTAT GGAGTCAAAC ACCGTAGCAA AATCAAAGAA CGACAAGGCA 
TGGAGGGCAG TCTCAGAGGA AATCAAGAGG ATCTTCAGAA CCTCTGTTTT ACAGCTGCAG 
GAGAAGGGGA CCATGAAGAG TGGTCAAGCC AAGAAATTTC TCTGCTCAGC ATTAGAGGAT 
GAACTGGACT TCGCATTGGG CAAGCAGACC CCAGCCTTTC TCAAAAAATG TGTTTGTTAC 
ATTCGGAAGA TATCTAACTT TGATCGTTTC GCTAAACTCC CCGAAATGGC ACGTTACATG 
GACATTGTGG TGAGCGATGA CCGTGTCATG AGAAACCAGG AAGCCTACGA GCGTCTGCTA 
AAGGTCAGAG ACGAGTTCAT TCCGGTGGTG GTGGCAGCAT CCAATCTCCG AGTCTACTCA 
TCGGTCACTC ACTGTGACAT GAAACTTGGC TACTCGCAGG AGGTAGAGAG CCATTACGTT 
GAAGGTCTGT GTAAACAGTT TTATGAAGAC ATGGTGGACA TCATCCAGGC GACGGTTCAG 
CAGAACTTTG ACACAGAGAC AGATCCTCTC TATGATGAAA TTATACAACA TTTGTCGCTG 
TGCAAGACGT TTTCATCTTT TTATGTGTAC AAGAGCGAAG CCTTGGATAT AGTCCAGGAG 
TACCTGTACC CCTCGAAAGG TGGTCGAATA ACTCCCCAAG TGGTTTACGG AGGACCCTGC 
ACTGGAAAGA CCTTGTTACT TGCTGAAGTG GCAAAACAGG CCTATTCATG GATACAGAAA 
GAGATGGGAC CAGAAACAGA CCCAGTGGTC ATTGTCAGAT TCATCGGTTC CACTGACTTC 
TCTACTGACC TCCGCACCCT GCTGCAGAGC ATCTGTGAGC AGATTGCCAT CAACTATCGG 
TGCCTGATCC ATTTCTTACC AAACAAGATC CAGGAGATGA GAGAACTGCT GGTGAATTTG 
CTGGGGGAGT CGTCATTCCA CCGACCTCTT GTCATTATTC TAGATGCCTT GGAGCAGTTG 
TCAGACGCAG ACGAGGCTCG AAAACTGTGG TGGCTCCCGG TCCACCTGCC ACGTACAGTT 
CGAATAGTAG TGTCCACCCT GCCCAACAAG CACGGAATAC TGCAGAAGCT GCGTTGCCTG 
ATACATGATG AGGAAGCCTA TGTTGAACTA ATGCAAAGAG ATCGCAAAGC TTGCAGTCAG 
ACCCTTAAAC AGCAGCTTCT GGGTGTCAAA AGGAAGGTGA CGTCTGGCCA ACAGATATAT 
GTTAATGAGG CAATGGCAAA ATGTACACTT CCTATGTTTG TTAACCTCAT CTACAGAGAG 
GTTGTTCACT GGAGATCGCA CAAGGATGTG GATGACAAAT CGTTGTGTTC CACAGTACAC 
GAAAGTATTG AGCAGCTTTT CTACTCAGTT GAAAATAAGC TTGGCTCACG CTTTGTCTTT 
CGGGCACTGG GCTACATCAC AATGGCACGG GCAGGCCTGA CTGAGCTGGA GCTAGAGGAT 
ATCTTATCTT TAGATAACAG TGTCCTAGGG GACATCATGG TGAGCTCTAA TCTCAAAAGC 
CCACTGCGTA TTTCCTATGA TTTCATAGCT CGGCTAAAAG AAGAACTTGA AGGTTATTTA 
GTGGAGCGTC AGGTCCATAA TGTAACACTA ATGGTGTGGG CAAATCGGCA TCTACATCTC 
ATAGCACAGA AGCTGTACCT CAGTAACGAA GAAGACGTGC ATCAAATGCA TAGCCTTTTG 
GCTGAGTACT TTCTTGGGGC ATGGGCAGGT GGCAGAAAGA AAATATTCCA CTGCGACAAT 
AATCACTTTG CCTCATTGAA TATCTCTCAG CATCGAAATC CTCACAACCA ACAGAGCCTT 
CATGGCCATG ACAAAGCTTC TGCTGACAAG CACTCATATG ACAGACAGAC ACCTGAGCAA 
CCCTGGGTGT TCCAATGTAA TCTGCTGGAG CCGGACATCT TCTTTGTCAA CCACCGTAAG 
ATGACAGAGC TCACATACCA CTTGACAAGG AGCGGCCGCA CAGATGATCT CATGTATGGT 
GTCATCATGA ACTTCAGTTG GCTCTACACT ATGATAAAGA TAGGGCAATT TGACAAGGCC 
CTGTCTGACA TTGACCTAGC CTACAGCTAT ACGCAAGAAA AGGAGCTAAA GTTTCTTGCA 
ACTACACTCC GCAGTATAAA GCTTAAAGTG ACAAAGAATC CAGCATCACT GTCTGCTGAA 
CTACAGCAAA GGCTCCTTCC AGTGGTCACA TCCCTTCCTA AACTCAGACA CCTCCTGCTT 
GAGTGTGACA AGGACGGCCC TAAATACTGC TCCATCGTGC CACTTCACTC CTCTATGGAT 
GTGACCTACA GTCCAGAGAG ATTGCCGCTC AGTTCTAGCT ACATGCAGAT AGTTGAGACT 
CTGCCTACAT TAGCTCCAAA CATAGTCATT GTGGCTCTTG AAGATGGGTC CGTCAGCACT 
TGGGATGTTG AAAGCAGACA GCTGCTAAGA CAGATAGACA CAGCAAGGTC AGTTGTTCTT 
GGAATTAGAC TAACCACTGA TGAGAAATAT CTGGTAGTGG CCACTACAAA AAATACACTG 
CTCATTTATG ACAACCACAA GTCTTGTTTG TTGTCTGAGG TAGAGGTCAA AGGGTCCAAA 
CACTGTGGGA TCACTGGTGG GGTGGCATTC ATTAATGGAT TTACCTTGTC CAGCCACCAT 
GCCTTAGCTT GGCTTGAGGC AAGCAAAGAT GTCAGTGTGA TTGATTTGCT GTACGGCTGG 
CCATTGTATC AGTTCCATTG CTGGTATGAG GTAACCTGTG TCCAGTGTTC CCCAGATGGA 
ATGTATGCTT TCTGTGGACA GTACCTCAAC ACAACCACAA TATTCCACCT TGGTAGTGGG 
GATAAGCTTG CCACAATGAC TTCTGAATTC TCAGGTGGTT TCGTGAAGTC CATTCTGATT 
CTAGACACAA TCAATCAAAT GGTGATGATT GACAATGAGG GCAGTCTCTC CGTATGGAGC 
ACCAAAGATA TCACAAACCC CAAGCTAATG GAGGATTATG ACTGCAGAGG AGATGATAGT 
GAGGTGGTGG GCATAGAGCT CTCAGAGGAT CAGCGATCCA TTCTAATCTG CAAAGCAAGA 
AGCATTGAGG TTCTGGACAC CAAAATTTGG AAGATGGCTG AAAAGTTTAA GGCCAAGCGC 
ACTGAGAAGT TTGTGGCAGC TGTTCTCTCC AAGAATAGCC AAAGTATAAT AGCCTCCATG 
GAGAACACAT CATCCATATT TGTGTGGAGG AGAGACAGTG GACAGTGCAT GGCCAGCTTG 
ATAGAGATAT CGGGAGCCAT TGTAAAGCTA ATCAAATCAA CACACCATAA CTTGCTTCTG 
TCTGTGGCCA GCAGTGGGGT GCTTTCCGTG TGGGATATCG ACATTATAAC AGCCATGTCG 
AACATTGATA AAACTGGAAA ACCTATCCAA ATTCTGCAAC TCTCTGGCAG AGAGGATTTT 
GTATACACCA TGGATGGGTC AGAAGTCATC CATAAATGGA ACTACAGCAC AGGTTTCATT 
GAAATGGTGT TCAAACATGA AGGACTTGTT GAGAACTGTG TCCTGACAAC ATCAGGTGAC 
CTAATGGTGA CATCTGACGA TAAAAGTAGT CAGTACATCT GGCATACGAC CACAGGTGAG 
AATATATTCC GTATCAATGG CCAGAAAATC TCACAGCTTC TCATCACGCA CAATGACCAA 
TTTGTTGTGT CACTTTGTGA GCAGAATGCC TCTCGTGTCT GGAGGCTTGC CACTGGTCAC 
AAGGTGTGCA ACATTCTGGT AACACTTCAA CATGCCCTCA TTACTACAGC AAACACATTC 
CTTGTGGGTG CAAACAAGAA TAAGCTGCTG GCTGTTAGCT TATGGTCAGG GAGTGTTTCC 
AAAAAGTTTA TCTGTGATGA TGGGATCACC ATTGTTAACT TCAAGTTAAT CCCTGACAGT 
CCAGATTTTG TTGTCTTCAT CACCTCCACT GAAACGGTAT TCATCTGGAG TGTTGCCGAT 
GAGTCAGTAT GTAGACGGGT GCAACTCCCC AACAACTTCT TAAAGAACCT GGAAGATTTC 
CAAATTTCAC CGAATGGGAA ATTGGGCATT GTCTCAAAAG GTGATGAGAA CATCAATGTT 
CTAGACCTAC ACAGTGGTAA GCTGCGTCTG GTGCATGCTG CTGGCATAAT ATGGCGGCAA 
AAGCTTTCAA GAGATGGACG CTACTTAGTA TACATCTGTT TCCGGAACTG CGATGAAGAT 
GATGATGCAG GTGTGGTTTC AAGCCTGATT GTCATGAGAC TGGCAGATGG TAAGAGTATC 
GGGACCTGTT CCCTTTACAA AACGCCAACC TATCTGTGCC TATCACAGCG AGCACTTAAC 
ATCATTGTCG GATTTGAGGA TGGCAGCATT GGCACTTATA CAGTGGTTGA TCGTGTAGAT 
GCTGCTCTGA AGATCAAGAT CGCCACCTCA AACAGTCGCC AAATTGTTAA CAACGCCTCA 
CAGAAAGTAC GACCCAAGTG TAGCAACAAT GCCTTCAAGA CCATTGCTGA CTGTGTGTGG 
AGAGAATCCA CTGAGGTCTT CTCTCGAGAC AGTCCTATCA ACGTGTCTGA TGGTGGGGAG 
CCAGAGACCA CCACACCCAC AAAGAAAACT GAACTGCTGC AATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nwd2 ( XM_009303553.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301828.1 Danio rerio NACHT and WD repeat domain containing 2 (nwd2), transcript variant X2, mRNA.

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CloneID	ODa43788
Clone ID Related Accession (Same CDS sequence)	XM_009303553.2 , XM_009303553.3
Accession Version	XM_009303553.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	NACHT and WD repeat domain-containing protein 2 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303553.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCCAT CCGGAGCGGG CAGCAGGCAG CCCTGTCCCC GTGAATCTGC ACTGCGCAGA 
GCAGCCATCA GTGGCAACGT CCTTGCCCTC CCACCATTCC ATGTCCCCAC GGGCAGGAGT 
GTCCGGGTCT TCATCTGTGC CAACCCTGAC GATACAGAAG CAGAGAGAAA TGCTCTGAAA 
GAACATGTCT ACCCCAAACT ACGAGACTTC TGCCGAGAGA ACTATGGCAT TGAATTCCAG 
GTGGTGGACC TGTACTGGGG CATTGATCCA GAGGAGTGGG ACAGTCCGGA GCTGCAGAGA 
CTGCGGATGA AACTACTGGA AGAATGTCTG AAGACTTCAG CTGGTCCATG CTTTGTTGGG 
CTCATAGGAG AGAAGTATGG CAGCATTCGG GTCCCAGGGG AGGTGGAGTC AGCTGAGTTT 
GAGATGATTT TGGATGCGGC AGTGGAGGCG GGACTAGACA CACGGATCTT GGAGGAGTGG 
TACTGTCGAG ATGAGAACTC AGTCCCACCT GCCTACTACC TAAAACCAAA AGCTGAGATG 
CTGAAAAACT ATCAGAATTC TATGGAGTCA AACACCGTAG CAAAATCAAA GAACGACAAG 
GCATGGAGGG CAGTCTCAGA GGAAATCAAG AGGATCTTCA GAACCTCTGT TTTACAGCTG 
CAGGAGAAGG GGACCATGAA GAGTGGTCAA GCCAAGAAAT TTCTCTGCTC AGCATTAGAG 
GATGAACTGG ACTTCGCATT GGGCAAGCAG ACCCCAGCCT TTCTCAAAAA ATGTGTTTGT 
TACATTCGGA AGATATCTAA CTTTGATCGT TTCGCTAAAC TCCCCGAAAT GGCACGTTAC 
ATGGACATTG TGGTGAGCGA TGACCGTGTC ATGAGAAACC AGGAAGCCTA CGAGCGTCTG 
CTAAAGGTCA GAGACGAGTT CATTCCGGTG GTGGTGGCAG CATCCAATCT CCGAGTCTAC 
TCATCGGTCA CTCACTGTGA CATGAAACTT GGCTACTCGC AGGAGGTAGA GAGCCATTAC 
GTTGAAGGTC TGTGTAAACA GTTTTATGAA GACATGGTGG ACATCATCCA GGCGACGGTT 
CAGCAGAACT TTGACACAGA GACAGATCCT CTCTATGATG AAATTATACA ACATTTGTCG 
CTGTGCAAGA CGTTTTCATC TTTTTATGTG TACAAGAGCG AAGCCTTGGA TATAGTCCAG 
GAGTACCTGT ACCCCTCGAA AGGTGGTCGA ATAACTCCCC AAGTGGTTTA CGGAGGACCC 
TGCACTGGAA AGACCTTGTT ACTTGCTGAA GTGGCAAAAC AGGCCTATTC ATGGATACAG 
AAAGAGATGG GACCAGAAAC AGACCCAGTG GTCATTGTCA GATTCATCGG TTCCACTGAC 
TTCTCTACTG ACCTCCGCAC CCTGCTGCAG AGCATCTGTG AGCAGATTGC CATCAACTAT 
CGGTGCCTGA TCCATTTCTT ACCAAACAAG ATCCAGGAGA TGAGAGAACT GCTGGTGAAT 
TTGCTGGGGG AGTCGTCATT CCACCGACCT CTTGTCATTA TTCTAGATGC CTTGGAGCAG 
TTGTCAGACG CAGACGAGGC TCGAAAACTG TGGTGGCTCC CGGTCCACCT GCCACGTACA 
GTTCGAATAG TAGTGTCCAC CCTGCCCAAC AAGCACGGAA TACTGCAGAA GCTGCGTTGC 
CTGATACATG ATGAGGAAGC CTATGTTGAA CTAATGCAAA GAGATCGCAA AGCTTGCAGT 
CAGACCCTTA AACAGCAGCT TCTGGGTGTC AAAAGGAAGG TGACGTCTGG CCAACAGATA 
TATGTTAATG AGGCAATGGC AAAATGTACA CTTCCTATGT TTGTTAACCT CATCTACAGA 
GAGGTTGTTC ACTGGAGATC GCACAAGGAT GTGGATGACA AATCGTTGTG TTCCACAGTA 
CACGAAAGTA TTGAGCAGCT TTTCTACTCA GTTGAAAATA AGCTTGGCTC ACGCTTTGTC 
TTTCGGGCAC TGGGCTACAT CACAATGGCA CGGGCAGGCC TGACTGAGCT GGAGCTAGAG 
GATATCTTAT CTTTAGATAA CAGTGTCCTA GGGGACATCA TGGTGAGCTC TAATCTCAAA 
AGCCCACTGC GTATTTCCTA TGATTTCATA GCTCGGCTAA AAGAAGAACT TGAAGGTTAT 
TTAGTGGAGC GTCAGGTCCA TAATGTAACA CTAATGGTGT GGGCAAATCG GCATCTACAT 
CTCATAGCAC AGAAGCTGTA CCTCAGTAAC GAAGAAGACG TGCATCAAAT GCATAGCCTT 
TTGGCTGAGT ACTTTCTTGG GGCATGGGCA GGTGGCAGAA AGAAAATATT CCACTGCGAC 
AATAATCACT TTGCCTCATT GAATATCTCT CAGCATCGAA ATCCTCACAA CCAACAGAGC 
CTTCATGGCC ATGACAAAGC TTCTGCTGAC AAGCACTCAT ATGACAGACA GACACCTGAG 
CAACCCTGGG TGTTCCAATG TAATCTGCTG GAGCCGGACA TCTTCTTTGT CAACCACCGT 
AAGATGACAG AGCTCACATA CCACTTGACA AGGAGCGGCC GCACAGATGA TCTCATGTAT 
GGTGTCATCA TGAACTTCAG TTGGCTCTAC ACTATGATAA AGATAGGGCA ATTTGACAAG 
GCCCTGTCTG ACATTGACCT AGCCTACAGC TATACGCAAG AAAAGGAGCT AAAGTTTCTT 
GCAACTACAC TCCGCAGTAT AAAGCTTAAA GTGACAAAGA ATCCAGCATC ACTGTCTGCT 
GAACTACAGC AAAGGCTCCT TCCAGTGGTC ACATCCCTTC CTAAACTCAG ACACCTCCTG 
CTTGAGTGTG ACAAGGACGG CCCTAAATAC TGCTCCATCG TGCCACTTCA CTCCTCTATG 
GATGTGACCT ACAGTCCAGA GAGATTGCCG CTCAGTTCTA GCTACATGCA GATAGTTGAG 
ACTCTGCCTA CATTAGCTCC AAACATAGTC ATTGTGGCTC TTGAAGATGG GTCCGTCAGC 
ACTTGGGATG TTGAAAGCAG ACAGCTGCTA AGACAGATAG ACACAGCAAG GTCAGTTGTT 
CTTGGAATTA GACTAACCAC TGATGAGAAA TATCTGGTAG TGGCCACTAC AAAAAATACA 
CTGCTCATTT ATGACAACCA CAAGTCTTGT TTGTTGTCTG AGGTAGAGGT CAAAGGGTCC 
AAACACTGTG GGATCACTGG TGGGGTGGCA TTCATTAATG GATTTACCTT GTCCAGCCAC 
CATGCCTTAG CTTGGCTTGA GGCAAGCAAA GATGTCAGTG TGATTGATTT GCTGTACGGC 
TGGCCATTGT ATCAGTTCCA TTGCTGGTAT GAGGTAACCT GTGTCCAGTG TTCCCCAGAT 
GGAATGTATG CTTTCTGTGG ACAGTACCTC AACACAACCA CAATATTCCA CCTTGGTAGT 
GGGGATAAGC TTGCCACAAT GACTTCTGAA TTCTCAGGTG GTTTCGTGAA GTCCATTCTG 
ATTCTAGACA CAATCAATCA AATGGTGATG ATTGACAATG AGGGCAGTCT CTCCGTATGG 
AGCACCAAAG ATATCACAAA CCCCAAGCTA ATGGAGGATT ATGACTGCAG AGGAGATGAT 
AGTGAGGTGG TGGGCATAGA GCTCTCAGAG GATCAGCGAT CCATTCTAAT CTGCAAAGCA 
AGAAGCATTG AGGTTCTGGA CACCAAAATT TGGAAGATGG CTGAAAAGTT TAAGGCCAAG 
CGCACTGAGA AGTTTGTGGC AGCTGTTCTC TCCAAGAATA GCCAAAGTAT AATAGCCTCC 
ATGGAGAACA CATCATCCAT ATTTGTGTGG AGGAGAGACA GTGGACAGTG CATGGCCAGC 
TTGATAGAGA TATCGGGAGC CATTGTAAAG CTAATCAAAT CAACACACCA TAACTTGCTT 
CTGTCTGTGG CCAGCAGTGG GGTGCTTTCC GTGTGGGATA TCGACATTAT AACAGCCATG 
TCGAACATTG ATAAAACTGG AAAACCTATC CAAATTCTGC AACTCTCTGG CAGAGAGGAT 
TTTGTATACA CCATGGATGG GTCAGAAGTC ATCCATAAAT GGAACTACAG CACAGGTTTC 
ATTGAAATGG TGTTCAAACA TGAAGGACTT GTTGAGAACT GTGTCCTGAC AACATCAGGT 
GACCTAATGG TGACATCTGA CGATAAAAGT AGTCAGTACA TCTGGCATAC GACCACAGGT 
GAGAATATAT TCCGTATCAA TGGCCAGAAA ATCTCACAGC TTCTCATCAC GCACAATGAC 
CAATTTGTTG TGTCACTTTG TGAGCAGAAT GCCTCTCGTG TCTGGAGGCT TGCCACTGGT 
CACAAGGTGT GCAACATTCT GGTAACACTT CAACATGCCC TCATTACTAC AGCAAACACA 
TTCCTTGTGG GTGCAAACAA GAATAAGCTG CTGGCTGTTA GCTTATGGTC AGGGAGTGTT 
TCCAAAAAGT TTATCTGTGA TGATGGGATC ACCATTGTTA ACTTCAAGTT AATCCCTGAC 
AGTCCAGATT TTGTTGTCTT CATCACCTCC ACTGAAACGG TATTCATCTG GAGTGTTGCC 
GATGAGTCAG TATGTAGACG GGTGCAACTC CCCAACAACT TCTTAAAGAA CCTGGAAGAT 
TTCCAAATTT CACCGAATGG GAAATTGGGC ATTGTCTCAA AAGGTGATGA GAACATCAAT 
GTTCTAGACC TACACAGTGG TAAGCTGCGT CTGGTGCATG CTGCTGGCAT AATATGGCGG 
CAAAAGCTTT CAAGAGATGG ACGCTACTTA GTATACATCT GTTTCCGGAA CTGCGATGAA 
GATGATGATG CAGGTGTGGT TTCAAGCCTG ATTGTCATGA GACTGGCAGA TGGTAAGAGT 
ATCGGGACCT GTTCCCTTTA CAAAACGCCA ACCTATCTGT GCCTATCACA GCGAGCACTT 
AACATCATTG TCGGATTTGA GGATGGCAGC ATTGGCACTT ATACAGTGGT TGATCGTGTA 
GATGCTGCTC TGAAGATCAA GATCGCCACC TCAAACAGTC GCCAAATTGT TAACAACGCC 
TCACAGAAAG TACGACCCAA GTGTAGCAAC AATGCCTTCA AGACCATTGC TGACTGTGTG 
TGGAGAGAAT CCACTGAGGT CTTCTCTCGA GACAGTCCTA TCAACGTGTC TGATGGTGGG 
GAGCCAGAGA CCACCACACC CACAAAGAAA ACTGAACTGC TGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301828.1
CDS	141..5348
Translation	MWPSGAGSRQPCPRESALRRAAISGNVLALPPFHVPTGRSVRVFICANPDDTEAERNALKEHVYPKLRDFCRENYGIEFQVVDLYWGIDPEEWDSPELQRLRMKLLEECLKTSAGPCFVGLIGEKYGSIRVPGEVESAEFEMILDAAVEAGLDTRILEEWYCRDENSVPPAYYLKPKAEMLKNYQNSMESNTVAKSKNDKAWRAVSEEIKRIFRTSVLQLQEKGTMKSGQAKKFLCSALEDELDFALGKQTPAFLKKCVCYIRKISNFDRFAKLPEMARYMDIVVSDDRVMRNQEAYERLLKVRDEFIPVVVAASNLRVYSSVTHCDMKLGYSQEVESHYVEGLCKQFYEDMVDIIQATVQQNFDTETDPLYDEIIQHLSLCKTFSSFYVYKSEALDIVQEYLYPSKGGRITPQVVYGGPCTGKTLLLAEVAKQAYSWIQKEMGPETDPVVIVRFIGSTDFSTDLRTLLQSICEQIAINYRCLIHFLPNKIQEMRELLVNLLGESSFHRPLVIILDALEQLSDADEARKLWWLPVHLPRTVRIVVSTLPNKHGILQKLRCLIHDEEAYVELMQRDRKACSQTLKQQLLGVKRKVTSGQQIYVNEAMAKCTLPMFVNLIYREVVHWRSHKDVDDKSLCSTVHESIEQLFYSVENKLGSRFVFRALGYITMARAGLTELELEDILSLDNSVLGDIMVSSNLKSPLRISYDFIARLKEELEGYLVERQVHNVTLMVWANRHLHLIAQKLYLSNEEDVHQMHSLLAEYFLGAWAGGRKKIFHCDNNHFASLNISQHRNPHNQQSLHGHDKASADKHSYDRQTPEQPWVFQCNLLEPDIFFVNHRKMTELTYHLTRSGRTDDLMYGVIMNFSWLYTMIKIGQFDKALSDIDLAYSYTQEKELKFLATTLRSIKLKVTKNPASLSAELQQRLLPVVTSLPKLRHLLLECDKDGPKYCSIVPLHSSMDVTYSPERLPLSSSYMQIVETLPTLAPNIVIVALEDGSVSTWDVESRQLLRQIDTARSVVLGIRLTTDEKYLVVATTKNTLLIYDNHKSCLLSEVEVKGSKHCGITGGVAFINGFTLSSHHALAWLEASKDVSVIDLLYGWPLYQFHCWYEVTCVQCSPDGMYAFCGQYLNTTTIFHLGSGDKLATMTSEFSGGFVKSILILDTINQMVMIDNEGSLSVWSTKDITNPKLMEDYDCRGDDSEVVGIELSEDQRSILICKARSIEVLDTKIWKMAEKFKAKRTEKFVAAVLSKNSQSIIASMENTSSIFVWRRDSGQCMASLIEISGAIVKLIKSTHHNLLLSVASSGVLSVWDIDIITAMSNIDKTGKPIQILQLSGREDFVYTMDGSEVIHKWNYSTGFIEMVFKHEGLVENCVLTTSGDLMVTSDDKSSQYIWHTTTGENIFRINGQKISQLLITHNDQFVVSLCEQNASRVWRLATGHKVCNILVTLQHALITTANTFLVGANKNKLLAVSLWSGSVSKKFICDDGITIVNFKLIPDSPDFVVFITSTETVFIWSVADESVCRRVQLPNNFLKNLEDFQISPNGKLGIVSKGDENINVLDLHSGKLRLVHAAGIIWRQKLSRDGRYLVYICFRNCDEDDDAGVVSSLIVMRLADGKSIGTCSLYKTPTYLCLSQRALNIIVGFEDGSIGTYTVVDRVDAALKIKIATSNSRQIVNNASQKVRPKCSNNAFKTIADCVWRESTEVFSRDSPINVSDGGEPETTTPTKKTELLQ

Target ORF information:

RefSeq Version	XM_009303553.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio NACHT and WD repeat domain containing 2 (nwd2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303553.2

ORF Insert Sequence:

ATGTGGCCAT CCGGAGCGGG CAGCAGGCAG CCCTGTCCCC GTGAATCTGC ACTGCGCAGA 
GCAGCCATCA GTGGCAACGT CCTTGCCCTC CCACCATTCC ATGTCCCCAC GGGCAGGAGT 
GTCCGGGTCT TCATCTGTGC CAACCCTGAC GATACAGAAG CAGAGAGAAA TGCTCTGAAA 
GAACATGTCT ACCCCAAACT ACGAGACTTC TGCCGAGAGA ACTATGGCAT TGAATTCCAG 
GTGGTGGACC TGTACTGGGG CATTGATCCA GAGGAGTGGG ACAGTCCGGA GCTGCAGAGA 
CTGCGGATGA AACTACTGGA AGAATGTCTG AAGACTTCAG CTGGTCCATG CTTTGTTGGG 
CTCATAGGAG AGAAGTATGG CAGCATTCGG GTCCCAGGGG AGGTGGAGTC AGCTGAGTTT 
GAGATGATTT TGGATGCGGC AGTGGAGGCG GGACTAGACA CACGGATCTT GGAGGAGTGG 
TACTGTCGAG ATGAGAACTC AGTCCCACCT GCCTACTACC TAAAACCAAA AGCTGAGATG 
CTGAAAAACT ATCAGAATTC TATGGAGTCA AACACCGTAG CAAAATCAAA GAACGACAAG 
GCATGGAGGG CAGTCTCAGA GGAAATCAAG AGGATCTTCA GAACCTCTGT TTTACAGCTG 
CAGGAGAAGG GGACCATGAA GAGTGGTCAA GCCAAGAAAT TTCTCTGCTC AGCATTAGAG 
GATGAACTGG ACTTCGCATT GGGCAAGCAG ACCCCAGCCT TTCTCAAAAA ATGTGTTTGT 
TACATTCGGA AGATATCTAA CTTTGATCGT TTCGCTAAAC TCCCCGAAAT GGCACGTTAC 
ATGGACATTG TGGTGAGCGA TGACCGTGTC ATGAGAAACC AGGAAGCCTA CGAGCGTCTG 
CTAAAGGTCA GAGACGAGTT CATTCCGGTG GTGGTGGCAG CATCCAATCT CCGAGTCTAC 
TCATCGGTCA CTCACTGTGA CATGAAACTT GGCTACTCGC AGGAGGTAGA GAGCCATTAC 
GTTGAAGGTC TGTGTAAACA GTTTTATGAA GACATGGTGG ACATCATCCA GGCGACGGTT 
CAGCAGAACT TTGACACAGA GACAGATCCT CTCTATGATG AAATTATACA ACATTTGTCG 
CTGTGCAAGA CGTTTTCATC TTTTTATGTG TACAAGAGCG AAGCCTTGGA TATAGTCCAG 
GAGTACCTGT ACCCCTCGAA AGGTGGTCGA ATAACTCCCC AAGTGGTTTA CGGAGGACCC 
TGCACTGGAA AGACCTTGTT ACTTGCTGAA GTGGCAAAAC AGGCCTATTC ATGGATACAG 
AAAGAGATGG GACCAGAAAC AGACCCAGTG GTCATTGTCA GATTCATCGG TTCCACTGAC 
TTCTCTACTG ACCTCCGCAC CCTGCTGCAG AGCATCTGTG AGCAGATTGC CATCAACTAT 
CGGTGCCTGA TCCATTTCTT ACCAAACAAG ATCCAGGAGA TGAGAGAACT GCTGGTGAAT 
TTGCTGGGGG AGTCGTCATT CCACCGACCT CTTGTCATTA TTCTAGATGC CTTGGAGCAG 
TTGTCAGACG CAGACGAGGC TCGAAAACTG TGGTGGCTCC CGGTCCACCT GCCACGTACA 
GTTCGAATAG TAGTGTCCAC CCTGCCCAAC AAGCACGGAA TACTGCAGAA GCTGCGTTGC 
CTGATACATG ATGAGGAAGC CTATGTTGAA CTAATGCAAA GAGATCGCAA AGCTTGCAGT 
CAGACCCTTA AACAGCAGCT TCTGGGTGTC AAAAGGAAGG TGACGTCTGG CCAACAGATA 
TATGTTAATG AGGCAATGGC AAAATGTACA CTTCCTATGT TTGTTAACCT CATCTACAGA 
GAGGTTGTTC ACTGGAGATC GCACAAGGAT GTGGATGACA AATCGTTGTG TTCCACAGTA 
CACGAAAGTA TTGAGCAGCT TTTCTACTCA GTTGAAAATA AGCTTGGCTC ACGCTTTGTC 
TTTCGGGCAC TGGGCTACAT CACAATGGCA CGGGCAGGCC TGACTGAGCT GGAGCTAGAG 
GATATCTTAT CTTTAGATAA CAGTGTCCTA GGGGACATCA TGGTGAGCTC TAATCTCAAA 
AGCCCACTGC GTATTTCCTA TGATTTCATA GCTCGGCTAA AAGAAGAACT TGAAGGTTAT 
TTAGTGGAGC GTCAGGTCCA TAATGTAACA CTAATGGTGT GGGCAAATCG GCATCTACAT 
CTCATAGCAC AGAAGCTGTA CCTCAGTAAC GAAGAAGACG TGCATCAAAT GCATAGCCTT 
TTGGCTGAGT ACTTTCTTGG GGCATGGGCA GGTGGCAGAA AGAAAATATT CCACTGCGAC 
AATAATCACT TTGCCTCATT GAATATCTCT CAGCATCGAA ATCCTCACAA CCAACAGAGC 
CTTCATGGCC ATGACAAAGC TTCTGCTGAC AAGCACTCAT ATGACAGACA GACACCTGAG 
CAACCCTGGG TGTTCCAATG TAATCTGCTG GAGCCGGACA TCTTCTTTGT CAACCACCGT 
AAGATGACAG AGCTCACATA CCACTTGACA AGGAGCGGCC GCACAGATGA TCTCATGTAT 
GGTGTCATCA TGAACTTCAG TTGGCTCTAC ACTATGATAA AGATAGGGCA ATTTGACAAG 
GCCCTGTCTG ACATTGACCT AGCCTACAGC TATACGCAAG AAAAGGAGCT AAAGTTTCTT 
GCAACTACAC TCCGCAGTAT AAAGCTTAAA GTGACAAAGA ATCCAGCATC ACTGTCTGCT 
GAACTACAGC AAAGGCTCCT TCCAGTGGTC ACATCCCTTC CTAAACTCAG ACACCTCCTG 
CTTGAGTGTG ACAAGGACGG CCCTAAATAC TGCTCCATCG TGCCACTTCA CTCCTCTATG 
GATGTGACCT ACAGTCCAGA GAGATTGCCG CTCAGTTCTA GCTACATGCA GATAGTTGAG 
ACTCTGCCTA CATTAGCTCC AAACATAGTC ATTGTGGCTC TTGAAGATGG GTCCGTCAGC 
ACTTGGGATG TTGAAAGCAG ACAGCTGCTA AGACAGATAG ACACAGCAAG GTCAGTTGTT 
CTTGGAATTA GACTAACCAC TGATGAGAAA TATCTGGTAG TGGCCACTAC AAAAAATACA 
CTGCTCATTT ATGACAACCA CAAGTCTTGT TTGTTGTCTG AGGTAGAGGT CAAAGGGTCC 
AAACACTGTG GGATCACTGG TGGGGTGGCA TTCATTAATG GATTTACCTT GTCCAGCCAC 
CATGCCTTAG CTTGGCTTGA GGCAAGCAAA GATGTCAGTG TGATTGATTT GCTGTACGGC 
TGGCCATTGT ATCAGTTCCA TTGCTGGTAT GAGGTAACCT GTGTCCAGTG TTCCCCAGAT 
GGAATGTATG CTTTCTGTGG ACAGTACCTC AACACAACCA CAATATTCCA CCTTGGTAGT 
GGGGATAAGC TTGCCACAAT GACTTCTGAA TTCTCAGGTG GTTTCGTGAA GTCCATTCTG 
ATTCTAGACA CAATCAATCA AATGGTGATG ATTGACAATG AGGGCAGTCT CTCCGTATGG 
AGCACCAAAG ATATCACAAA CCCCAAGCTA ATGGAGGATT ATGACTGCAG AGGAGATGAT 
AGTGAGGTGG TGGGCATAGA GCTCTCAGAG GATCAGCGAT CCATTCTAAT CTGCAAAGCA 
AGAAGCATTG AGGTTCTGGA CACCAAAATT TGGAAGATGG CTGAAAAGTT TAAGGCCAAG 
CGCACTGAGA AGTTTGTGGC AGCTGTTCTC TCCAAGAATA GCCAAAGTAT AATAGCCTCC 
ATGGAGAACA CATCATCCAT ATTTGTGTGG AGGAGAGACA GTGGACAGTG CATGGCCAGC 
TTGATAGAGA TATCGGGAGC CATTGTAAAG CTAATCAAAT CAACACACCA TAACTTGCTT 
CTGTCTGTGG CCAGCAGTGG GGTGCTTTCC GTGTGGGATA TCGACATTAT AACAGCCATG 
TCGAACATTG ATAAAACTGG AAAACCTATC CAAATTCTGC AACTCTCTGG CAGAGAGGAT 
TTTGTATACA CCATGGATGG GTCAGAAGTC ATCCATAAAT GGAACTACAG CACAGGTTTC 
ATTGAAATGG TGTTCAAACA TGAAGGACTT GTTGAGAACT GTGTCCTGAC AACATCAGGT 
GACCTAATGG TGACATCTGA CGATAAAAGT AGTCAGTACA TCTGGCATAC GACCACAGGT 
GAGAATATAT TCCGTATCAA TGGCCAGAAA ATCTCACAGC TTCTCATCAC GCACAATGAC 
CAATTTGTTG TGTCACTTTG TGAGCAGAAT GCCTCTCGTG TCTGGAGGCT TGCCACTGGT 
CACAAGGTGT GCAACATTCT GGTAACACTT CAACATGCCC TCATTACTAC AGCAAACACA 
TTCCTTGTGG GTGCAAACAA GAATAAGCTG CTGGCTGTTA GCTTATGGTC AGGGAGTGTT 
TCCAAAAAGT TTATCTGTGA TGATGGGATC ACCATTGTTA ACTTCAAGTT AATCCCTGAC 
AGTCCAGATT TTGTTGTCTT CATCACCTCC ACTGAAACGG TATTCATCTG GAGTGTTGCC 
GATGAGTCAG TATGTAGACG GGTGCAACTC CCCAACAACT TCTTAAAGAA CCTGGAAGAT 
TTCCAAATTT CACCGAATGG GAAATTGGGC ATTGTCTCAA AAGGTGATGA GAACATCAAT 
GTTCTAGACC TACACAGTGG TAAGCTGCGT CTGGTGCATG CTGCTGGCAT AATATGGCGG 
CAAAAGCTTT CAAGAGATGG ACGCTACTTA GTATACATCT GTTTCCGGAA CTGCGATGAA 
GATGATGATG CAGGTGTGGT TTCAAGCCTG ATTGTCATGA GACTGGCAGA TGGTAAGAGT 
ATCGGGACCT GTTCCCTTTA CAAAACGCCA ACCTATCTGT GCCTATCACA GCGAGCACTT 
AACATCATTG TCGGATTTGA GGATGGCAGC ATTGGCACTT ATACAGTGGT TGATCGTGTA 
GATGCTGCTC TGAAGATCAA GATCGCCACC TCAAACAGTC GCCAAATTGT TAACAACGCC 
TCACAGAAAG TACGACCCAA GTGTAGCAAC AATGCCTTCA AGACCATTGC TGACTGTGTG 
TGGAGAGAAT CCACTGAGGT CTTCTCTCGA GACAGTCCTA TCAACGTGTC TGATGGTGGG 
GAGCCAGAGA CCACCACACC CACAAAGAAA ACTGAACTGC TGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

nwd2 ( XM_009303553.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301828.1 Danio rerio NACHT and WD repeat domain containing 2 (nwd2), transcript variant X2, mRNA.

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CloneID	ODa43788
Clone ID Related Accession (Same CDS sequence)	XM_009303553.2 , XM_009303553.3
Accession Version	XM_009303553.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	NACHT and WD repeat domain-containing protein 2 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303553.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGCCAT CCGGAGCGGG CAGCAGGCAG CCCTGTCCCC GTGAATCTGC ACTGCGCAGA 
GCAGCCATCA GTGGCAACGT CCTTGCCCTC CCACCATTCC ATGTCCCCAC GGGCAGGAGT 
GTCCGGGTCT TCATCTGTGC CAACCCTGAC GATACAGAAG CAGAGAGAAA TGCTCTGAAA 
GAACATGTCT ACCCCAAACT ACGAGACTTC TGCCGAGAGA ACTATGGCAT TGAATTCCAG 
GTGGTGGACC TGTACTGGGG CATTGATCCA GAGGAGTGGG ACAGTCCGGA GCTGCAGAGA 
CTGCGGATGA AACTACTGGA AGAATGTCTG AAGACTTCAG CTGGTCCATG CTTTGTTGGG 
CTCATAGGAG AGAAGTATGG CAGCATTCGG GTCCCAGGGG AGGTGGAGTC AGCTGAGTTT 
GAGATGATTT TGGATGCGGC AGTGGAGGCG GGACTAGACA CACGGATCTT GGAGGAGTGG 
TACTGTCGAG ATGAGAACTC AGTCCCACCT GCCTACTACC TAAAACCAAA AGCTGAGATG 
CTGAAAAACT ATCAGAATTC TATGGAGTCA AACACCGTAG CAAAATCAAA GAACGACAAG 
GCATGGAGGG CAGTCTCAGA GGAAATCAAG AGGATCTTCA GAACCTCTGT TTTACAGCTG 
CAGGAGAAGG GGACCATGAA GAGTGGTCAA GCCAAGAAAT TTCTCTGCTC AGCATTAGAG 
GATGAACTGG ACTTCGCATT GGGCAAGCAG ACCCCAGCCT TTCTCAAAAA ATGTGTTTGT 
TACATTCGGA AGATATCTAA CTTTGATCGT TTCGCTAAAC TCCCCGAAAT GGCACGTTAC 
ATGGACATTG TGGTGAGCGA TGACCGTGTC ATGAGAAACC AGGAAGCCTA CGAGCGTCTG 
CTAAAGGTCA GAGACGAGTT CATTCCGGTG GTGGTGGCAG CATCCAATCT CCGAGTCTAC 
TCATCGGTCA CTCACTGTGA CATGAAACTT GGCTACTCGC AGGAGGTAGA GAGCCATTAC 
GTTGAAGGTC TGTGTAAACA GTTTTATGAA GACATGGTGG ACATCATCCA GGCGACGGTT 
CAGCAGAACT TTGACACAGA GACAGATCCT CTCTATGATG AAATTATACA ACATTTGTCG 
CTGTGCAAGA CGTTTTCATC TTTTTATGTG TACAAGAGCG AAGCCTTGGA TATAGTCCAG 
GAGTACCTGT ACCCCTCGAA AGGTGGTCGA ATAACTCCCC AAGTGGTTTA CGGAGGACCC 
TGCACTGGAA AGACCTTGTT ACTTGCTGAA GTGGCAAAAC AGGCCTATTC ATGGATACAG 
AAAGAGATGG GACCAGAAAC AGACCCAGTG GTCATTGTCA GATTCATCGG TTCCACTGAC 
TTCTCTACTG ACCTCCGCAC CCTGCTGCAG AGCATCTGTG AGCAGATTGC CATCAACTAT 
CGGTGCCTGA TCCATTTCTT ACCAAACAAG ATCCAGGAGA TGAGAGAACT GCTGGTGAAT 
TTGCTGGGGG AGTCGTCATT CCACCGACCT CTTGTCATTA TTCTAGATGC CTTGGAGCAG 
TTGTCAGACG CAGACGAGGC TCGAAAACTG TGGTGGCTCC CGGTCCACCT GCCACGTACA 
GTTCGAATAG TAGTGTCCAC CCTGCCCAAC AAGCACGGAA TACTGCAGAA GCTGCGTTGC 
CTGATACATG ATGAGGAAGC CTATGTTGAA CTAATGCAAA GAGATCGCAA AGCTTGCAGT 
CAGACCCTTA AACAGCAGCT TCTGGGTGTC AAAAGGAAGG TGACGTCTGG CCAACAGATA 
TATGTTAATG AGGCAATGGC AAAATGTACA CTTCCTATGT TTGTTAACCT CATCTACAGA 
GAGGTTGTTC ACTGGAGATC GCACAAGGAT GTGGATGACA AATCGTTGTG TTCCACAGTA 
CACGAAAGTA TTGAGCAGCT TTTCTACTCA GTTGAAAATA AGCTTGGCTC ACGCTTTGTC 
TTTCGGGCAC TGGGCTACAT CACAATGGCA CGGGCAGGCC TGACTGAGCT GGAGCTAGAG 
GATATCTTAT CTTTAGATAA CAGTGTCCTA GGGGACATCA TGGTGAGCTC TAATCTCAAA 
AGCCCACTGC GTATTTCCTA TGATTTCATA GCTCGGCTAA AAGAAGAACT TGAAGGTTAT 
TTAGTGGAGC GTCAGGTCCA TAATGTAACA CTAATGGTGT GGGCAAATCG GCATCTACAT 
CTCATAGCAC AGAAGCTGTA CCTCAGTAAC GAAGAAGACG TGCATCAAAT GCATAGCCTT 
TTGGCTGAGT ACTTTCTTGG GGCATGGGCA GGTGGCAGAA AGAAAATATT CCACTGCGAC 
AATAATCACT TTGCCTCATT GAATATCTCT CAGCATCGAA ATCCTCACAA CCAACAGAGC 
CTTCATGGCC ATGACAAAGC TTCTGCTGAC AAGCACTCAT ATGACAGACA GACACCTGAG 
CAACCCTGGG TGTTCCAATG TAATCTGCTG GAGCCGGACA TCTTCTTTGT CAACCACCGT 
AAGATGACAG AGCTCACATA CCACTTGACA AGGAGCGGCC GCACAGATGA TCTCATGTAT 
GGTGTCATCA TGAACTTCAG TTGGCTCTAC ACTATGATAA AGATAGGGCA ATTTGACAAG 
GCCCTGTCTG ACATTGACCT AGCCTACAGC TATACGCAAG AAAAGGAGCT AAAGTTTCTT 
GCAACTACAC TCCGCAGTAT AAAGCTTAAA GTGACAAAGA ATCCAGCATC ACTGTCTGCT 
GAACTACAGC AAAGGCTCCT TCCAGTGGTC ACATCCCTTC CTAAACTCAG ACACCTCCTG 
CTTGAGTGTG ACAAGGACGG CCCTAAATAC TGCTCCATCG TGCCACTTCA CTCCTCTATG 
GATGTGACCT ACAGTCCAGA GAGATTGCCG CTCAGTTCTA GCTACATGCA GATAGTTGAG 
ACTCTGCCTA CATTAGCTCC AAACATAGTC ATTGTGGCTC TTGAAGATGG GTCCGTCAGC 
ACTTGGGATG TTGAAAGCAG ACAGCTGCTA AGACAGATAG ACACAGCAAG GTCAGTTGTT 
CTTGGAATTA GACTAACCAC TGATGAGAAA TATCTGGTAG TGGCCACTAC AAAAAATACA 
CTGCTCATTT ATGACAACCA CAAGTCTTGT TTGTTGTCTG AGGTAGAGGT CAAAGGGTCC 
AAACACTGTG GGATCACTGG TGGGGTGGCA TTCATTAATG GATTTACCTT GTCCAGCCAC 
CATGCCTTAG CTTGGCTTGA GGCAAGCAAA GATGTCAGTG TGATTGATTT GCTGTACGGC 
TGGCCATTGT ATCAGTTCCA TTGCTGGTAT GAGGTAACCT GTGTCCAGTG TTCCCCAGAT 
GGAATGTATG CTTTCTGTGG ACAGTACCTC AACACAACCA CAATATTCCA CCTTGGTAGT 
GGGGATAAGC TTGCCACAAT GACTTCTGAA TTCTCAGGTG GTTTCGTGAA GTCCATTCTG 
ATTCTAGACA CAATCAATCA AATGGTGATG ATTGACAATG AGGGCAGTCT CTCCGTATGG 
AGCACCAAAG ATATCACAAA CCCCAAGCTA ATGGAGGATT ATGACTGCAG AGGAGATGAT 
AGTGAGGTGG TGGGCATAGA GCTCTCAGAG GATCAGCGAT CCATTCTAAT CTGCAAAGCA 
AGAAGCATTG AGGTTCTGGA CACCAAAATT TGGAAGATGG CTGAAAAGTT TAAGGCCAAG 
CGCACTGAGA AGTTTGTGGC AGCTGTTCTC TCCAAGAATA GCCAAAGTAT AATAGCCTCC 
ATGGAGAACA CATCATCCAT ATTTGTGTGG AGGAGAGACA GTGGACAGTG CATGGCCAGC 
TTGATAGAGA TATCGGGAGC CATTGTAAAG CTAATCAAAT CAACACACCA TAACTTGCTT 
CTGTCTGTGG CCAGCAGTGG GGTGCTTTCC GTGTGGGATA TCGACATTAT AACAGCCATG 
TCGAACATTG ATAAAACTGG AAAACCTATC CAAATTCTGC AACTCTCTGG CAGAGAGGAT 
TTTGTATACA CCATGGATGG GTCAGAAGTC ATCCATAAAT GGAACTACAG CACAGGTTTC 
ATTGAAATGG TGTTCAAACA TGAAGGACTT GTTGAGAACT GTGTCCTGAC AACATCAGGT 
GACCTAATGG TGACATCTGA CGATAAAAGT AGTCAGTACA TCTGGCATAC GACCACAGGT 
GAGAATATAT TCCGTATCAA TGGCCAGAAA ATCTCACAGC TTCTCATCAC GCACAATGAC 
CAATTTGTTG TGTCACTTTG TGAGCAGAAT GCCTCTCGTG TCTGGAGGCT TGCCACTGGT 
CACAAGGTGT GCAACATTCT GGTAACACTT CAACATGCCC TCATTACTAC AGCAAACACA 
TTCCTTGTGG GTGCAAACAA GAATAAGCTG CTGGCTGTTA GCTTATGGTC AGGGAGTGTT 
TCCAAAAAGT TTATCTGTGA TGATGGGATC ACCATTGTTA ACTTCAAGTT AATCCCTGAC 
AGTCCAGATT TTGTTGTCTT CATCACCTCC ACTGAAACGG TATTCATCTG GAGTGTTGCC 
GATGAGTCAG TATGTAGACG GGTGCAACTC CCCAACAACT TCTTAAAGAA CCTGGAAGAT 
TTCCAAATTT CACCGAATGG GAAATTGGGC ATTGTCTCAA AAGGTGATGA GAACATCAAT 
GTTCTAGACC TACACAGTGG TAAGCTGCGT CTGGTGCATG CTGCTGGCAT AATATGGCGG 
CAAAAGCTTT CAAGAGATGG ACGCTACTTA GTATACATCT GTTTCCGGAA CTGCGATGAA 
GATGATGATG CAGGTGTGGT TTCAAGCCTG ATTGTCATGA GACTGGCAGA TGGTAAGAGT 
ATCGGGACCT GTTCCCTTTA CAAAACGCCA ACCTATCTGT GCCTATCACA GCGAGCACTT 
AACATCATTG TCGGATTTGA GGATGGCAGC ATTGGCACTT ATACAGTGGT TGATCGTGTA 
GATGCTGCTC TGAAGATCAA GATCGCCACC TCAAACAGTC GCCAAATTGT TAACAACGCC 
TCACAGAAAG TACGACCCAA GTGTAGCAAC AATGCCTTCA AGACCATTGC TGACTGTGTG 
TGGAGAGAAT CCACTGAGGT CTTCTCTCGA GACAGTCCTA TCAACGTGTC TGATGGTGGG 
GAGCCAGAGA CCACCACACC CACAAAGAAA ACTGAACTGC TGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009301828.1
CDS	441..5648
Translation	MWPSGAGSRQPCPRESALRRAAISGNVLALPPFHVPTGRSVRVFICANPDDTEAERNALKEHVYPKLRDFCRENYGIEFQVVDLYWGIDPEEWDSPELQRLRMKLLEECLKTSAGPCFVGLIGEKYGSIRVPGEVESAEFEMILDAAVEAGLDTRILEEWYCRDENSVPPAYYLKPKAEMLKNYQNSMESNTVAKSKNDKAWRAVSEEIKRIFRTSVLQLQEKGTMKSGQAKKFLCSALEDELDFALGKQTPAFLKKCVCYIRKISNFDRFAKLPEMARYMDIVVSDDRVMRNQEAYERLLKVRDEFIPVVVAASNLRVYSSVTHCDMKLGYSQEVESHYVEGLCKQFYEDMVDIIQATVQQNFDTETDPLYDEIIQHLSLCKTFSSFYVYKSEALDIVQEYLYPSKGGRITPQVVYGGPCTGKTLLLAEVAKQAYSWIQKEMGPETDPVVIVRFIGSTDFSTDLRTLLQSICEQIAINYRCLIHFLPNKIQEMRELLVNLLGESSFHRPLVIILDALEQLSDADEARKLWWLPVHLPRTVRIVVSTLPNKHGILQKLRCLIHDEEAYVELMQRDRKACSQTLKQQLLGVKRKVTSGQQIYVNEAMAKCTLPMFVNLIYREVVHWRSHKDVDDKSLCSTVHESIEQLFYSVENKLGSRFVFRALGYITMARAGLTELELEDILSLDNSVLGDIMVSSNLKSPLRISYDFIARLKEELEGYLVERQVHNVTLMVWANRHLHLIAQKLYLSNEEDVHQMHSLLAEYFLGAWAGGRKKIFHCDNNHFASLNISQHRNPHNQQSLHGHDKASADKHSYDRQTPEQPWVFQCNLLEPDIFFVNHRKMTELTYHLTRSGRTDDLMYGVIMNFSWLYTMIKIGQFDKALSDIDLAYSYTQEKELKFLATTLRSIKLKVTKNPASLSAELQQRLLPVVTSLPKLRHLLLECDKDGPKYCSIVPLHSSMDVTYSPERLPLSSSYMQIVETLPTLAPNIVIVALEDGSVSTWDVESRQLLRQIDTARSVVLGIRLTTDEKYLVVATTKNTLLIYDNHKSCLLSEVEVKGSKHCGITGGVAFINGFTLSSHHALAWLEASKDVSVIDLLYGWPLYQFHCWYEVTCVQCSPDGMYAFCGQYLNTTTIFHLGSGDKLATMTSEFSGGFVKSILILDTINQMVMIDNEGSLSVWSTKDITNPKLMEDYDCRGDDSEVVGIELSEDQRSILICKARSIEVLDTKIWKMAEKFKAKRTEKFVAAVLSKNSQSIIASMENTSSIFVWRRDSGQCMASLIEISGAIVKLIKSTHHNLLLSVASSGVLSVWDIDIITAMSNIDKTGKPIQILQLSGREDFVYTMDGSEVIHKWNYSTGFIEMVFKHEGLVENCVLTTSGDLMVTSDDKSSQYIWHTTTGENIFRINGQKISQLLITHNDQFVVSLCEQNASRVWRLATGHKVCNILVTLQHALITTANTFLVGANKNKLLAVSLWSGSVSKKFICDDGITIVNFKLIPDSPDFVVFITSTETVFIWSVADESVCRRVQLPNNFLKNLEDFQISPNGKLGIVSKGDENINVLDLHSGKLRLVHAAGIIWRQKLSRDGRYLVYICFRNCDEDDDAGVVSSLIVMRLADGKSIGTCSLYKTPTYLCLSQRALNIIVGFEDGSIGTYTVVDRVDAALKIKIATSNSRQIVNNASQKVRPKCSNNAFKTIADCVWRESTEVFSRDSPINVSDGGEPETTTPTKKTELLQ

Target ORF information:

RefSeq Version	XM_009303553.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NACHT and WD repeat domain containing 2 (nwd2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009303553.3

ORF Insert Sequence:

ATGTGGCCAT CCGGAGCGGG CAGCAGGCAG CCCTGTCCCC GTGAATCTGC ACTGCGCAGA 
GCAGCCATCA GTGGCAACGT CCTTGCCCTC CCACCATTCC ATGTCCCCAC GGGCAGGAGT 
GTCCGGGTCT TCATCTGTGC CAACCCTGAC GATACAGAAG CAGAGAGAAA TGCTCTGAAA 
GAACATGTCT ACCCCAAACT ACGAGACTTC TGCCGAGAGA ACTATGGCAT TGAATTCCAG 
GTGGTGGACC TGTACTGGGG CATTGATCCA GAGGAGTGGG ACAGTCCGGA GCTGCAGAGA 
CTGCGGATGA AACTACTGGA AGAATGTCTG AAGACTTCAG CTGGTCCATG CTTTGTTGGG 
CTCATAGGAG AGAAGTATGG CAGCATTCGG GTCCCAGGGG AGGTGGAGTC AGCTGAGTTT 
GAGATGATTT TGGATGCGGC AGTGGAGGCG GGACTAGACA CACGGATCTT GGAGGAGTGG 
TACTGTCGAG ATGAGAACTC AGTCCCACCT GCCTACTACC TAAAACCAAA AGCTGAGATG 
CTGAAAAACT ATCAGAATTC TATGGAGTCA AACACCGTAG CAAAATCAAA GAACGACAAG 
GCATGGAGGG CAGTCTCAGA GGAAATCAAG AGGATCTTCA GAACCTCTGT TTTACAGCTG 
CAGGAGAAGG GGACCATGAA GAGTGGTCAA GCCAAGAAAT TTCTCTGCTC AGCATTAGAG 
GATGAACTGG ACTTCGCATT GGGCAAGCAG ACCCCAGCCT TTCTCAAAAA ATGTGTTTGT 
TACATTCGGA AGATATCTAA CTTTGATCGT TTCGCTAAAC TCCCCGAAAT GGCACGTTAC 
ATGGACATTG TGGTGAGCGA TGACCGTGTC ATGAGAAACC AGGAAGCCTA CGAGCGTCTG 
CTAAAGGTCA GAGACGAGTT CATTCCGGTG GTGGTGGCAG CATCCAATCT CCGAGTCTAC 
TCATCGGTCA CTCACTGTGA CATGAAACTT GGCTACTCGC AGGAGGTAGA GAGCCATTAC 
GTTGAAGGTC TGTGTAAACA GTTTTATGAA GACATGGTGG ACATCATCCA GGCGACGGTT 
CAGCAGAACT TTGACACAGA GACAGATCCT CTCTATGATG AAATTATACA ACATTTGTCG 
CTGTGCAAGA CGTTTTCATC TTTTTATGTG TACAAGAGCG AAGCCTTGGA TATAGTCCAG 
GAGTACCTGT ACCCCTCGAA AGGTGGTCGA ATAACTCCCC AAGTGGTTTA CGGAGGACCC 
TGCACTGGAA AGACCTTGTT ACTTGCTGAA GTGGCAAAAC AGGCCTATTC ATGGATACAG 
AAAGAGATGG GACCAGAAAC AGACCCAGTG GTCATTGTCA GATTCATCGG TTCCACTGAC 
TTCTCTACTG ACCTCCGCAC CCTGCTGCAG AGCATCTGTG AGCAGATTGC CATCAACTAT 
CGGTGCCTGA TCCATTTCTT ACCAAACAAG ATCCAGGAGA TGAGAGAACT GCTGGTGAAT 
TTGCTGGGGG AGTCGTCATT CCACCGACCT CTTGTCATTA TTCTAGATGC CTTGGAGCAG 
TTGTCAGACG CAGACGAGGC TCGAAAACTG TGGTGGCTCC CGGTCCACCT GCCACGTACA 
GTTCGAATAG TAGTGTCCAC CCTGCCCAAC AAGCACGGAA TACTGCAGAA GCTGCGTTGC 
CTGATACATG ATGAGGAAGC CTATGTTGAA CTAATGCAAA GAGATCGCAA AGCTTGCAGT 
CAGACCCTTA AACAGCAGCT TCTGGGTGTC AAAAGGAAGG TGACGTCTGG CCAACAGATA 
TATGTTAATG AGGCAATGGC AAAATGTACA CTTCCTATGT TTGTTAACCT CATCTACAGA 
GAGGTTGTTC ACTGGAGATC GCACAAGGAT GTGGATGACA AATCGTTGTG TTCCACAGTA 
CACGAAAGTA TTGAGCAGCT TTTCTACTCA GTTGAAAATA AGCTTGGCTC ACGCTTTGTC 
TTTCGGGCAC TGGGCTACAT CACAATGGCA CGGGCAGGCC TGACTGAGCT GGAGCTAGAG 
GATATCTTAT CTTTAGATAA CAGTGTCCTA GGGGACATCA TGGTGAGCTC TAATCTCAAA 
AGCCCACTGC GTATTTCCTA TGATTTCATA GCTCGGCTAA AAGAAGAACT TGAAGGTTAT 
TTAGTGGAGC GTCAGGTCCA TAATGTAACA CTAATGGTGT GGGCAAATCG GCATCTACAT 
CTCATAGCAC AGAAGCTGTA CCTCAGTAAC GAAGAAGACG TGCATCAAAT GCATAGCCTT 
TTGGCTGAGT ACTTTCTTGG GGCATGGGCA GGTGGCAGAA AGAAAATATT CCACTGCGAC 
AATAATCACT TTGCCTCATT GAATATCTCT CAGCATCGAA ATCCTCACAA CCAACAGAGC 
CTTCATGGCC ATGACAAAGC TTCTGCTGAC AAGCACTCAT ATGACAGACA GACACCTGAG 
CAACCCTGGG TGTTCCAATG TAATCTGCTG GAGCCGGACA TCTTCTTTGT CAACCACCGT 
AAGATGACAG AGCTCACATA CCACTTGACA AGGAGCGGCC GCACAGATGA TCTCATGTAT 
GGTGTCATCA TGAACTTCAG TTGGCTCTAC ACTATGATAA AGATAGGGCA ATTTGACAAG 
GCCCTGTCTG ACATTGACCT AGCCTACAGC TATACGCAAG AAAAGGAGCT AAAGTTTCTT 
GCAACTACAC TCCGCAGTAT AAAGCTTAAA GTGACAAAGA ATCCAGCATC ACTGTCTGCT 
GAACTACAGC AAAGGCTCCT TCCAGTGGTC ACATCCCTTC CTAAACTCAG ACACCTCCTG 
CTTGAGTGTG ACAAGGACGG CCCTAAATAC TGCTCCATCG TGCCACTTCA CTCCTCTATG 
GATGTGACCT ACAGTCCAGA GAGATTGCCG CTCAGTTCTA GCTACATGCA GATAGTTGAG 
ACTCTGCCTA CATTAGCTCC AAACATAGTC ATTGTGGCTC TTGAAGATGG GTCCGTCAGC 
ACTTGGGATG TTGAAAGCAG ACAGCTGCTA AGACAGATAG ACACAGCAAG GTCAGTTGTT 
CTTGGAATTA GACTAACCAC TGATGAGAAA TATCTGGTAG TGGCCACTAC AAAAAATACA 
CTGCTCATTT ATGACAACCA CAAGTCTTGT TTGTTGTCTG AGGTAGAGGT CAAAGGGTCC 
AAACACTGTG GGATCACTGG TGGGGTGGCA TTCATTAATG GATTTACCTT GTCCAGCCAC 
CATGCCTTAG CTTGGCTTGA GGCAAGCAAA GATGTCAGTG TGATTGATTT GCTGTACGGC 
TGGCCATTGT ATCAGTTCCA TTGCTGGTAT GAGGTAACCT GTGTCCAGTG TTCCCCAGAT 
GGAATGTATG CTTTCTGTGG ACAGTACCTC AACACAACCA CAATATTCCA CCTTGGTAGT 
GGGGATAAGC TTGCCACAAT GACTTCTGAA TTCTCAGGTG GTTTCGTGAA GTCCATTCTG 
ATTCTAGACA CAATCAATCA AATGGTGATG ATTGACAATG AGGGCAGTCT CTCCGTATGG 
AGCACCAAAG ATATCACAAA CCCCAAGCTA ATGGAGGATT ATGACTGCAG AGGAGATGAT 
AGTGAGGTGG TGGGCATAGA GCTCTCAGAG GATCAGCGAT CCATTCTAAT CTGCAAAGCA 
AGAAGCATTG AGGTTCTGGA CACCAAAATT TGGAAGATGG CTGAAAAGTT TAAGGCCAAG 
CGCACTGAGA AGTTTGTGGC AGCTGTTCTC TCCAAGAATA GCCAAAGTAT AATAGCCTCC 
ATGGAGAACA CATCATCCAT ATTTGTGTGG AGGAGAGACA GTGGACAGTG CATGGCCAGC 
TTGATAGAGA TATCGGGAGC CATTGTAAAG CTAATCAAAT CAACACACCA TAACTTGCTT 
CTGTCTGTGG CCAGCAGTGG GGTGCTTTCC GTGTGGGATA TCGACATTAT AACAGCCATG 
TCGAACATTG ATAAAACTGG AAAACCTATC CAAATTCTGC AACTCTCTGG CAGAGAGGAT 
TTTGTATACA CCATGGATGG GTCAGAAGTC ATCCATAAAT GGAACTACAG CACAGGTTTC 
ATTGAAATGG TGTTCAAACA TGAAGGACTT GTTGAGAACT GTGTCCTGAC AACATCAGGT 
GACCTAATGG TGACATCTGA CGATAAAAGT AGTCAGTACA TCTGGCATAC GACCACAGGT 
GAGAATATAT TCCGTATCAA TGGCCAGAAA ATCTCACAGC TTCTCATCAC GCACAATGAC 
CAATTTGTTG TGTCACTTTG TGAGCAGAAT GCCTCTCGTG TCTGGAGGCT TGCCACTGGT 
CACAAGGTGT GCAACATTCT GGTAACACTT CAACATGCCC TCATTACTAC AGCAAACACA 
TTCCTTGTGG GTGCAAACAA GAATAAGCTG CTGGCTGTTA GCTTATGGTC AGGGAGTGTT 
TCCAAAAAGT TTATCTGTGA TGATGGGATC ACCATTGTTA ACTTCAAGTT AATCCCTGAC 
AGTCCAGATT TTGTTGTCTT CATCACCTCC ACTGAAACGG TATTCATCTG GAGTGTTGCC 
GATGAGTCAG TATGTAGACG GGTGCAACTC CCCAACAACT TCTTAAAGAA CCTGGAAGAT 
TTCCAAATTT CACCGAATGG GAAATTGGGC ATTGTCTCAA AAGGTGATGA GAACATCAAT 
GTTCTAGACC TACACAGTGG TAAGCTGCGT CTGGTGCATG CTGCTGGCAT AATATGGCGG 
CAAAAGCTTT CAAGAGATGG ACGCTACTTA GTATACATCT GTTTCCGGAA CTGCGATGAA 
GATGATGATG CAGGTGTGGT TTCAAGCCTG ATTGTCATGA GACTGGCAGA TGGTAAGAGT 
ATCGGGACCT GTTCCCTTTA CAAAACGCCA ACCTATCTGT GCCTATCACA GCGAGCACTT 
AACATCATTG TCGGATTTGA GGATGGCAGC ATTGGCACTT ATACAGTGGT TGATCGTGTA 
GATGCTGCTC TGAAGATCAA GATCGCCACC TCAAACAGTC GCCAAATTGT TAACAACGCC 
TCACAGAAAG TACGACCCAA GTGTAGCAAC AATGCCTTCA AGACCATTGC TGACTGTGTG 
TGGAGAGAAT CCACTGAGGT CTTCTCTCGA GACAGTCCTA TCAACGTGTC TGATGGTGGG 
GAGCCAGAGA CCACCACACC CACAAAGAAA ACTGAACTGC TGCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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オンライン注文

nwd2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム

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