ACSS2L cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ACSS2L
Entrez Gene ID	560020
Full Name	acyl-CoA synthetase short chain family member 2 like
Synonyms	zmp:0000000720
General protein information	Preferred Names acyl-CoA synthetase short chain family member 2 like Names acetyl-coenzyme A synthetase, cytoplasmic acs
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001277117.2	NP_001264046.2	acetyl-coenzyme A synthetase, cytoplasmic
NM_001277117.1	NP_001264046.1	acetyl-coenzyme A synthetase, cytoplasmic

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	acss2l	NP_001264046.1
Xenopus tropicalis (tropical clawed frog)	LOC100492069	XP_002937518.1
Magnaporthe oryzae (rice blast fungus)	MGG_04590	XP_003710902.1

Gene Ontology
Bibliography

Related articles in PubMed

ATF4 overexpression induces early onset of hyperlipidaemia and hepatic steatosis and enhances adipogenesis in zebrafish.
Yeh KY, Lai CY, Lin CY, Hsu CC, Lo CP, Her GM
Scientific reports7(1)16362(2017 Nov)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Ubiquitous transcription factor YY1 promotes zebrafish liver steatosis and lipotoxicity by inhibiting CHOP-10 expression.
Her GM, Pai WY, Lai CY, Hsieh YW, Pang HW
Biochimica et biophysica acta1831(6)1037-51(2013 Jun)

Regulation of cytochrome P450 expression in a novel liver cell line from zebrafish (Brachydanio rerio).
Miranda CL, Collodi P, Zhao X, Barnes DW, Buhler DR
Archives of biochemistry and biophysics305(2)320-7(1993 Sep)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ACSS2L gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ACSS2L cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa35927	NM_001277117.1 Latest version!	Danio rerio acyl-CoA synthetase short-chain family member 2 like (acss2l), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa141190	NM_001277117.2 Latest version!	Danio rerio acyl-CoA synthetase short chain family member 2 like (acss2l), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ACSS2L ( NM_001277117.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001264046.1 Danio rerio acyl-CoA synthetase short-chain family member 2 like (acss2l), mRNA.

Price & Availability↑

CloneID	ODa35927
Clone ID Related Accession (Same CDS sequence)	NM_001277117.1
Accession Version	NM_001277117.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-08-27
Organism	Danio rerio(Zebrafish)
Product	acetyl-coenzyme A synthetase, cytoplasmic
Comment	VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BC129436.1 and CABZ01087855.1. On Mar 10, 2013 this sequence version replaced XM_001919016.3. Sequence Note: This RefSeq record was created from transcript and genomic sequence data of mixed strains. No single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and homologous protein data. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMN02418768, SAMN02418769 [ECO:0000350] ##Evidence-Data-END##

ATGTCTCCCG TTTCAAACTC CACCAACACG GCTCTTTCAA GCCCACAGGA GCCAGAGGAG 
AAGATTTACC ATCCACCCGA CTGTATTTTC ACTGACGCGC ACGTGCCTGA CTTTGACGCA 
TATCTGGCTT TATATAAAAA GTCCATTGAA GAGCCTGAAG AGTTCTGGGG TGATGTTGCT 
CAGGAGTTTT TCTGGAAGAA GCCTCCGTCG GGACAGATGT TGCAGTATAA CTTCAACGTC 
ACCAAGGGGA GTGTTTTCAT CAAGTTCATG GACGCAGCAA AAACCAACAT TTGCTACAAC 
GTCCTCGACC GGAATGTGCA CGAGAGAAAC CTGGGAGACA AAGTGGCTTA TTACTGGGTG 
GGAAACTCAC CTGATCATCA TCTAACCATC ACCTACAACC AGCTGTTGAG AAACGTGTGC 
CGATGTGCCA ATGCTTTAAA GCTGTTGGGG GTGAAGAAGG GGGACAGAGT CGCCATTTAT 
TTACCCATGA TTCCAGAGCT GGTGTACACC ATGCTGGCCT GCGCGAGAAT CGGGGCTGTT 
CACTCTATAG TGTTTGCAGG TTTCTCGTCC GAGTCTCTGT GTGAAAGGAT CATGGACGCT 
CAATGCAACA TCCTGGTCAC AGCAGATGGT GGTTACAGAG GTGACAAGCT GATCAACCTG 
AAGAAGATAG CGTCTGAAGC TTTGGATCGC TGTAAGGAAA GCTCGGCCTC GTCTGTTCAG 
AAGTGTGTTG TGGTCAAACA TCCTGCCTTC ATAACCAGCA GCGATGCTTC CAGCAGTCTA 
CAGACTCCAT GGGATGAATT GTGTGATATC TGGTGGGATG ACCTGATGGA TGGAGTGTCG 
GATGAGTGTG AACCGGAGTG GATGGACTCT GAAGATCCTC TTTTCATACT TTACACCAGC 
GGCTCCACCG GAAAACCCAA GGGGGTGGTC CACACCGTCA CAGGCTACAT GCTCTACACA 
TCACTGACCT TCAAATATGT TTTCGATTAT CACCATGACG ACGTTTACTG GTGCACTGCA 
GACATTGGCT GGATCACAGG CCACTCCTAC ATCACATATG GCCCGCTGGC CAATGGCGCG 
ACCAGTGTGC TCTTTGAAGG TGTGCCGGTC CATCCTCATG TGGGCAGACT CTGGGAGATC 
ATTGAGAAGT ATGGAGTCTC TAAGTTCTAC ACGGCGCCCA CTGCTATAAG ACTGCTGATG 
AAATATGGGA AAGAACCACT GCAGCGGTAT GATCTGTCCA GCTTGCGTGT TTTGGGAACG 
GTTGGTGAAC CCATTAACCC AGAGGCTTGG CTTTGGTATT ATGATGTTGT GGGTCAGCAG 
CGCTGTCCGG TTATCGACAC CTTCTGGCAA ACAGAGACGG GTGGTCATGT TCTGACTCCT 
CTACCAGCTG CTACACCACT AAAACCTGGA TCTGCTACGT GCCCATTTTT TGGAGTCCAG 
CCTGCCATTC TTAATGAACA CGGAGAAGAA TTAGAGGGCG AAGCGGAGGG ATACTTGGTT 
TTCCGGCAGC CCTGGCCGGG AATCATGAGG GGTGTATTTG GGAATCAGGA GCGATTCGAG 
AGCACATACT TCAGAAAATT CCCAGGATTC TACGTGACAG GAGACGGCTG CAGGCGGGAC 
AAGGATGGAT ATTACTGGAT CACCGGCCGG ATTGATGACA TGCTGAATGT TTCTGGTCAT 
TTGCTGAGCA CAGCAGAGGT GGAGTCTGCT CTGTCTGAAC ACCCATCGGT GGCTGAAGCT 
GCAGTGGTCA GTTGTCCTCA TGCAGTGAAA GGAGAGTGTC TGTACTGCTT TATCACTCTG 
AAAGACCAGC AAGAGTTCAA CCGCACGCTG ATGGATGAAC TCAAGAGGAA AGTGAGGGAG 
AAGATTGGAC CCATCGCCAC CCCAGATTTT ATCCAGAACG CTCCAGGGCT TCCCAAAACC 
CGTTCAGGGA AGATCATGCG ACGAGTTCTA CGACAGATCG CTCGCAATGA AAAAGAGCTG 
GGTGACCTGT CTACACTGGC GGACCCTAAA GTTGTGGAGG TGCTGTTCAG CCAGAGATGT 
GAAGCAGCGG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001264046.1
CDS	41..2095
Translation	MSPVSNSTNTALSSPQEPEEKIYHPPDCIFTDAHVPDFDAYLALYKKSIEEPEEFWGDVAQEFFWKKPPSGQMLQYNFNVTKGSVFIKFMDAAKTNICYNVLDRNVHERNLGDKVAYYWVGNSPDHHLTITYNQLLRNVCRCANALKLLGVKKGDRVAIYLPMIPELVYTMLACARIGAVHSIVFAGFSSESLCERIMDAQCNILVTADGGYRGDKLINLKKIASEALDRCKESSASSVQKCVVVKHPAFITSSDASSSLQTPWDELCDIWWDDLMDGVSDECEPEWMDSEDPLFILYTSGSTGKPKGVVHTVTGYMLYTSLTFKYVFDYHHDDVYWCTADIGWITGHSYITYGPLANGATSVLFEGVPVHPHVGRLWEIIEKYGVSKFYTAPTAIRLLMKYGKEPLQRYDLSSLRVLGTVGEPINPEAWLWYYDVVGQQRCPVIDTFWQTETGGHVLTPLPAATPLKPGSATCPFFGVQPAILNEHGEELEGEAEGYLVFRQPWPGIMRGVFGNQERFESTYFRKFPGFYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALSEHPSVAEAAVVSCPHAVKGECLYCFITLKDQQEFNRTLMDELKRKVREKIGPIATPDFIQNAPGLPKTRSGKIMRRVLRQIARNEKELGDLSTLADPKVVEVLFSQRCEAAA

Target ORF information:

RefSeq Version	NM_001277117.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio acyl-CoA synthetase short-chain family member 2 like (acss2l), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001277117.1

ORF Insert Sequence:

ATGTCTCCCG TTTCAAACTC CACCAACACG GCTCTTTCAA GCCCACAGGA GCCAGAGGAG 
AAGATTTACC ATCCACCCGA CTGTATTTTC ACTGACGCGC ACGTGCCTGA CTTTGACGCA 
TATCTGGCTT TATATAAAAA GTCCATTGAA GAGCCTGAAG AGTTCTGGGG TGATGTTGCT 
CAGGAGTTTT TCTGGAAGAA GCCTCCGTCG GGACAGATGT TGCAGTATAA CTTCAACGTC 
ACCAAGGGGA GTGTTTTCAT CAAGTTCATG GACGCAGCAA AAACCAACAT TTGCTACAAC 
GTCCTCGACC GGAATGTGCA CGAGAGAAAC CTGGGAGACA AAGTGGCTTA TTACTGGGTG 
GGAAACTCAC CTGATCATCA TCTAACCATC ACCTACAACC AGCTGTTGAG AAACGTGTGC 
CGATGTGCCA ATGCTTTAAA GCTGTTGGGG GTGAAGAAGG GGGACAGAGT CGCCATTTAT 
TTACCCATGA TTCCAGAGCT GGTGTACACC ATGCTGGCCT GCGCGAGAAT CGGGGCTGTT 
CACTCTATAG TGTTTGCAGG TTTCTCGTCC GAGTCTCTGT GTGAAAGGAT CATGGACGCT 
CAATGCAACA TCCTGGTCAC AGCAGATGGT GGTTACAGAG GTGACAAGCT GATCAACCTG 
AAGAAGATAG CGTCTGAAGC TTTGGATCGC TGTAAGGAAA GCTCGGCCTC GTCTGTTCAG 
AAGTGTGTTG TGGTCAAACA TCCTGCCTTC ATAACCAGCA GCGATGCTTC CAGCAGTCTA 
CAGACTCCAT GGGATGAATT GTGTGATATC TGGTGGGATG ACCTGATGGA TGGAGTGTCG 
GATGAGTGTG AACCGGAGTG GATGGACTCT GAAGATCCTC TTTTCATACT TTACACCAGC 
GGCTCCACCG GAAAACCCAA GGGGGTGGTC CACACCGTCA CAGGCTACAT GCTCTACACA 
TCACTGACCT TCAAATATGT TTTCGATTAT CACCATGACG ACGTTTACTG GTGCACTGCA 
GACATTGGCT GGATCACAGG CCACTCCTAC ATCACATATG GCCCGCTGGC CAATGGCGCG 
ACCAGTGTGC TCTTTGAAGG TGTGCCGGTC CATCCTCATG TGGGCAGACT CTGGGAGATC 
ATTGAGAAGT ATGGAGTCTC TAAGTTCTAC ACGGCGCCCA CTGCTATAAG ACTGCTGATG 
AAATATGGGA AAGAACCACT GCAGCGGTAT GATCTGTCCA GCTTGCGTGT TTTGGGAACG 
GTTGGTGAAC CCATTAACCC AGAGGCTTGG CTTTGGTATT ATGATGTTGT GGGTCAGCAG 
CGCTGTCCGG TTATCGACAC CTTCTGGCAA ACAGAGACGG GTGGTCATGT TCTGACTCCT 
CTACCAGCTG CTACACCACT AAAACCTGGA TCTGCTACGT GCCCATTTTT TGGAGTCCAG 
CCTGCCATTC TTAATGAACA CGGAGAAGAA TTAGAGGGCG AAGCGGAGGG ATACTTGGTT 
TTCCGGCAGC CCTGGCCGGG AATCATGAGG GGTGTATTTG GGAATCAGGA GCGATTCGAG 
AGCACATACT TCAGAAAATT CCCAGGATTC TACGTGACAG GAGACGGCTG CAGGCGGGAC 
AAGGATGGAT ATTACTGGAT CACCGGCCGG ATTGATGACA TGCTGAATGT TTCTGGTCAT 
TTGCTGAGCA CAGCAGAGGT GGAGTCTGCT CTGTCTGAAC ACCCATCGGT GGCTGAAGCT 
GCAGTGGTCA GTTGTCCTCA TGCAGTGAAA GGAGAGTGTC TGTACTGCTT TATCACTCTG 
AAAGACCAGC AAGAGTTCAA CCGCACGCTG ATGGATGAAC TCAAGAGGAA AGTGAGGGAG 
AAGATTGGAC CCATCGCCAC CCCAGATTTT ATCCAGAACG CTCCAGGGCT TCCCAAAACC 
CGTTCAGGGA AGATCATGCG ACGAGTTCTA CGACAGATCG CTCGCAATGA AAAAGAGCTG 
GGTGACCTGT CTACACTGGC GGACCCTAAA GTTGTGGAGG TGCTGTTCAG CCAGAGATGT 
GAAGCAGCGG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ACSS2L ( NM_001277117.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001264046.2 Danio rerio acyl-CoA synthetase short chain family member 2 like (acss2l), mRNA.

Price & Availability↑

CloneID	ODa141190
Clone ID Related Accession (Same CDS sequence)	NM_001277117.2
Accession Version	NM_001277117.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2055bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2020-01-04
Organism	Danio rerio(zebrafish)
Product	acetyl-coenzyme A synthetase, cytoplasmic
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CABZ01087853.1, BC129436.1, CABZ01087854.1 and CABZ01087855.1. On Aug 4, 2017 this sequence version replaced NM_001277117.1. Sequence Note: This RefSeq record was created from transcript and genomic sequence data of mixed strains. No single transcript from the same strain was available for the full length of the gene. The extent of this transcript is supported by transcript alignments and homologous protein data. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGTCTCCCG TTTCAGACTC CACCAACACG GCTCTTTCAA GCCCAAAGGA GCCCGAGGAG 
AAGATTTACC ATCCACCCGA CGGCATTTTC ACTGACGCGC ACGTGCCTGA CTTTGACGCA 
TATCTGGCTT TATATAAAAA GTCCATTGAA GAGCCTGAAG AGTTCTGGGG TGATGTTGCT 
CAGGAGTTTT TCTGGAAGAA GCCTCCGTCG GGACAGATGT TGCAGTATAA CTTCGACGTC 
ACCAAGGGGA GTGTTTTCAT CAAGTTCATG GACGGAGCAA AAACCAACAT TTGCTACAAC 
GTCCTCGACC GGAATGTGCA TGAGAGAAAC CTGGGAGACA AAGTGGCTTA TTACTGGGTG 
GGAAACTCAC CTGATCATCA TCTAACCATC ACCTACAACC AGCTGTTGAG AAACGTGTGC 
CGATGTGCCA ATGCTTTAAA GCTGTTGGGG GTGAAGAAGG GGGACAGAGT CGCCATTTAT 
TTACCCATGA TTCCAGAGCT GGTGTACACC ATGCTGGCCT GCGCGAGAAT CGGGGCTGTT 
CACTCTATAG TGTTTGCAGG TTTCTCGTCC GAGTCTCTGT GTGAAAGGAT CATGGACGCT 
CAATGCAACA TCCTGGTCAC AGCAGATGGT GTTTACAGAG GTGACAAGCT GATCAACCTG 
AAGAAGATAG CGTCTGAAGC TTTGGATCGC TGTAAGGAAA GCTCGGCCTC GTCTGTTCAG 
AAGTGTGTTG TGGTCAAACA TCCTGCCTTC ATAACCAGCA GCGATGCTTC CAGCAGTCTA 
CAGACTCCAT GGGATGAATT GTGTGATATC TGGTGGGATG ACCTGATGGA TGGAGTGTCG 
GATGAGTGTG AACCGGAGTG GATGGACTCT GAAGATCCTC TTTTCATACT TTACACCAGC 
GGCTCCACCG GAAAACCCAA GGGGGTGGTC CACACCGTCA CAGGCTACAT GCTCTACACC 
TCACTGACCT TCAAATATGT TTTCGATTAT CACCATGACG ACGTTTACTG GTGCACTGCA 
GACATTGGCT GGATCACAGG CCACTCCTAC ATCACATATG GCCCGCTGGC CAATGGCGCG 
ACCAGTGTGC TCTTTGAAGG TGTGCCGGTC CATCCTCATG TGGGCAGACT CTGGGAGATC 
ATTGAGAAGT ATGGAGTCTC TAAGTTCTAC ACGGCGCCCA CTGCTATAAG ACTGCTGATG 
AAATATGGGA AAGAACCACT GCAGCGGTAT GATCTGTCCA GCTTGCGTGT TTTGGGAACG 
GTTGGTGAAC CCATTAACCC AGAGGCTTGG CTTTGGTATT ATGATGTTGT GGGTCAGCAG 
CGCTGTCCGG TTATCGACAC CTTCTGGCAA ACAGAGACGG GTGGTCATGT TCTGACTCCT 
CTACCAGCTG CTACACCACT AAAACCTGGA TCTGCTACGT GCCCATTTTT TGGAGTCCAG 
CCTGCCATTC TTAATGAACA CGGAGAAGAA TTAGAGGGCG AAGCGGAGGG ATACTTGGTT 
TTCCGGCAGC CCTGGCCGGG AATCATGAGG GGTGTATTTG GGAATCAGGA GCGATTCGAG 
AGCACATACT TCAGAAAATT CCCAGGATTC TACGTGACAG GAGACGGCTG CAGGCGGGAC 
AAGGATGGAT ATTACTGGAT CACCGGCCGG ATTGATGACA TGCTGAATGT TTCTGGTCAT 
TTGCTGAGCA CAGCAGAGGT GGAGTCTGCT CTGTCTGAAC ACCCATCGGT GGCTGAAGCT 
GCAGTGGTCA GTTGTCCTCA TGCAGTGAAA GGAGAGTGTC TGTACTGCTT TATCACTCTG 
AAAGACCAGC AAGAGTTCAA CCGCACGCTG ATGGATGAAC TCAAGAGGAA AGTGAGGGAG 
AAGATTGGAC CCATCGCCAC CCCAGATTTT ATCCAGAACG CTCCAGGGCT TCCCAAAACC 
CGTTCAGGGA AGATCATGCG ACGAGTTCTA CGACAGATCG CTCGCAATGA AAAAGAGCTG 
GGTGACCTGT CTACACTGGC GGACCCTAAA GTTGTGGAGG TGCTGTTCAG CCAGAGATGT 
GAAGCAGCGG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001264046.2
CDS	41..2095
Translation	MSPVSDSTNTALSSPKEPEEKIYHPPDGIFTDAHVPDFDAYLALYKKSIEEPEEFWGDVAQEFFWKKPPSGQMLQYNFDVTKGSVFIKFMDGAKTNICYNVLDRNVHERNLGDKVAYYWVGNSPDHHLTITYNQLLRNVCRCANALKLLGVKKGDRVAIYLPMIPELVYTMLACARIGAVHSIVFAGFSSESLCERIMDAQCNILVTADGVYRGDKLINLKKIASEALDRCKESSASSVQKCVVVKHPAFITSSDASSSLQTPWDELCDIWWDDLMDGVSDECEPEWMDSEDPLFILYTSGSTGKPKGVVHTVTGYMLYTSLTFKYVFDYHHDDVYWCTADIGWITGHSYITYGPLANGATSVLFEGVPVHPHVGRLWEIIEKYGVSKFYTAPTAIRLLMKYGKEPLQRYDLSSLRVLGTVGEPINPEAWLWYYDVVGQQRCPVIDTFWQTETGGHVLTPLPAATPLKPGSATCPFFGVQPAILNEHGEELEGEAEGYLVFRQPWPGIMRGVFGNQERFESTYFRKFPGFYVTGDGCRRDKDGYYWITGRIDDMLNVSGHLLSTAEVESALSEHPSVAEAAVVSCPHAVKGECLYCFITLKDQQEFNRTLMDELKRKVREKIGPIATPDFIQNAPGLPKTRSGKIMRRVLRQIARNEKELGDLSTLADPKVVEVLFSQRCEAAA

Target ORF information:

RefSeq Version	NM_001277117.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio acyl-CoA synthetase short chain family member 2 like (acss2l), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001277117.2

ORF Insert Sequence:

ATGTCTCCCG TTTCAGACTC CACCAACACG GCTCTTTCAA GCCCAAAGGA GCCCGAGGAG 
AAGATTTACC ATCCACCCGA CGGCATTTTC ACTGACGCGC ACGTGCCTGA CTTTGACGCA 
TATCTGGCTT TATATAAAAA GTCCATTGAA GAGCCTGAAG AGTTCTGGGG TGATGTTGCT 
CAGGAGTTTT TCTGGAAGAA GCCTCCGTCG GGACAGATGT TGCAGTATAA CTTCGACGTC 
ACCAAGGGGA GTGTTTTCAT CAAGTTCATG GACGGAGCAA AAACCAACAT TTGCTACAAC 
GTCCTCGACC GGAATGTGCA TGAGAGAAAC CTGGGAGACA AAGTGGCTTA TTACTGGGTG 
GGAAACTCAC CTGATCATCA TCTAACCATC ACCTACAACC AGCTGTTGAG AAACGTGTGC 
CGATGTGCCA ATGCTTTAAA GCTGTTGGGG GTGAAGAAGG GGGACAGAGT CGCCATTTAT 
TTACCCATGA TTCCAGAGCT GGTGTACACC ATGCTGGCCT GCGCGAGAAT CGGGGCTGTT 
CACTCTATAG TGTTTGCAGG TTTCTCGTCC GAGTCTCTGT GTGAAAGGAT CATGGACGCT 
CAATGCAACA TCCTGGTCAC AGCAGATGGT GTTTACAGAG GTGACAAGCT GATCAACCTG 
AAGAAGATAG CGTCTGAAGC TTTGGATCGC TGTAAGGAAA GCTCGGCCTC GTCTGTTCAG 
AAGTGTGTTG TGGTCAAACA TCCTGCCTTC ATAACCAGCA GCGATGCTTC CAGCAGTCTA 
CAGACTCCAT GGGATGAATT GTGTGATATC TGGTGGGATG ACCTGATGGA TGGAGTGTCG 
GATGAGTGTG AACCGGAGTG GATGGACTCT GAAGATCCTC TTTTCATACT TTACACCAGC 
GGCTCCACCG GAAAACCCAA GGGGGTGGTC CACACCGTCA CAGGCTACAT GCTCTACACC 
TCACTGACCT TCAAATATGT TTTCGATTAT CACCATGACG ACGTTTACTG GTGCACTGCA 
GACATTGGCT GGATCACAGG CCACTCCTAC ATCACATATG GCCCGCTGGC CAATGGCGCG 
ACCAGTGTGC TCTTTGAAGG TGTGCCGGTC CATCCTCATG TGGGCAGACT CTGGGAGATC 
ATTGAGAAGT ATGGAGTCTC TAAGTTCTAC ACGGCGCCCA CTGCTATAAG ACTGCTGATG 
AAATATGGGA AAGAACCACT GCAGCGGTAT GATCTGTCCA GCTTGCGTGT TTTGGGAACG 
GTTGGTGAAC CCATTAACCC AGAGGCTTGG CTTTGGTATT ATGATGTTGT GGGTCAGCAG 
CGCTGTCCGG TTATCGACAC CTTCTGGCAA ACAGAGACGG GTGGTCATGT TCTGACTCCT 
CTACCAGCTG CTACACCACT AAAACCTGGA TCTGCTACGT GCCCATTTTT TGGAGTCCAG 
CCTGCCATTC TTAATGAACA CGGAGAAGAA TTAGAGGGCG AAGCGGAGGG ATACTTGGTT 
TTCCGGCAGC CCTGGCCGGG AATCATGAGG GGTGTATTTG GGAATCAGGA GCGATTCGAG 
AGCACATACT TCAGAAAATT CCCAGGATTC TACGTGACAG GAGACGGCTG CAGGCGGGAC 
AAGGATGGAT ATTACTGGAT CACCGGCCGG ATTGATGACA TGCTGAATGT TTCTGGTCAT 
TTGCTGAGCA CAGCAGAGGT GGAGTCTGCT CTGTCTGAAC ACCCATCGGT GGCTGAAGCT 
GCAGTGGTCA GTTGTCCTCA TGCAGTGAAA GGAGAGTGTC TGTACTGCTT TATCACTCTG 
AAAGACCAGC AAGAGTTCAA CCGCACGCTG ATGGATGAAC TCAAGAGGAA AGTGAGGGAG 
AAGATTGGAC CCATCGCCAC CCCAGATTTT ATCCAGAACG CTCCAGGGCT TCCCAAAACC 
CGTTCAGGGA AGATCATGCG ACGAGTTCTA CGACAGATCG CTCGCAATGA AAAAGAGCTG 
GGTGACCTGT CTACACTGGC GGACCCTAAA GTTGTGGAGG TGCTGTTCAG CCAGAGATGT 
GAAGCAGCGG CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ATF4 overexpression induces early onset of hyperlipidaemia and hepatic steatosis and enhances adipogenesis in zebrafish.
Scientific reports7(1)16362(2017 Nov)
Yeh KY,Lai CY,Lin CY,Hsu CC,Lo CP,Her GM

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Ubiquitous transcription factor YY1 promotes zebrafish liver steatosis and lipotoxicity by inhibiting CHOP-10 expression.
Biochimica et biophysica acta1831(6)1037-51(2013 Jun)
Her GM,Pai WY,Lai CY,Hsieh YW,Pang HW

Regulation of cytochrome P450 expression in a novel liver cell line from zebrafish (Brachydanio rerio).
Archives of biochemistry and biophysics305(2)320-7(1993 Sep)
Miranda CL,Collodi P,Zhao X,Barnes DW,Buhler DR

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ACSS2L cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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