arhgef1b cDNA ORF clone, Danio rerio(zebrafish)

The following arhgef1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the arhgef1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa149250 XM_021466706.1
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 1b (arhgef1b), transcript variant X10, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
ODa149241 XM_021466698.1
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 1b (arhgef1b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa149242 XM_021466699.1
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 1b (arhgef1b), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa149243 XM_021466700.1
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 1b (arhgef1b), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa149244 XM_021466701.1
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 1b (arhgef1b), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa149245 XM_021466702.1
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 1b (arhgef1b), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa149246 XM_017351352.2
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 1b (arhgef1b), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa149247 XM_021466703.1
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 1b (arhgef1b), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa149248 XM_021466704.1
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 1b (arhgef1b), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa149249 XM_021466705.1
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 1b (arhgef1b), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa55380 XM_017351352.1
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 1b (arhgef1b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $629.30
$899.00
ODa42711 NM_001044848.2
Latest version!
Danio rerio Rho guanine nucleotide exchange factor (GEF) 1b (arhgef1b), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa149250
    Clone ID Related Accession (Same CDS sequence) XM_021466706.1
    Accession Version XM_021466706.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2589bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 1 isoform X10
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGTTACATA ATTGTAAACG CCCCTCAATT GTTCCTGACA AGGACAAAAG TAACTCCATC 
    TTTGGAGCAG TGGCTTATTA TATGAAGTAT CTTGGAGTCA AGACCAGAGC TTTTGACAAC
    AAGAAGTCCA AGTCAGGATG GCGGCCCTCG GTTCCATCTA TGCTGAAGCC ATGGTCAGTC
    AACAAGCAGA TTAAACCAGT CAAACCGCCT GGAATCGGCT ATGACGGTAA GCAAAGTAGA
    TTGGACTTTG AAGCAAAGCA AAACAAACAA CCTCCTCATC GAGTGTCTGT TAGTGATAGT
    GTCACCCCTC CAGGGCGAAA AACTGGTATT ACCGTGTCAG GGAGCACTCA TGGGTCAGAG
    AGCAGTGAAG AGTTACCCAT CAGTGTGAGT GTGACTCCCA GCCCTGACTT TCAAACTGAT
    ACAGTAGGTA TCATCAACAA TCGCACAGAC CCCAAACCAG CCTCTGAGGG TGGAGAAGTG
    TCTCCAGTCA GCACTGGTGG GACTGTGTCC ATCTGTGAAC CTCTCGCTGT CATTGATGTC
    CCTCCAGAAG ACATTCAGGA CAGCGGCAGA AAGAAGCCAA GGAATGTGCG TCGCAGTGAA
    AGCCTTTGTG TGGAGCGGCG TCACTCTCGT CGTGGTGGCT CTACTCGGGC CAAGCAGTCT
    CGTTCCCGCA GCGACGTGGA CCTCCAGTCA ACTTCCACAT CCCATCCCCT CTCACCCTCC
    CCACAGCATT CAGTCTCCGT GGAAGGTGAC AAAACACCGG AAATCGCTCT CCTCACTCCC
    ACTTTTGTCC CGGCCCCTCA GCAAGAAGAG ATAGAGCCCC GTCTCCTGGA GCTCGAGCAG
    GACCCACTCA ACTGGAGAGA ACTTGCTTCC AGTGAAAGCC TGCATAAGCT GAGCAAGAAG
    GAAGCCAAAA GACAAGAAGT CATAAATGAG CTGTTTGTGA CAGAACAAGC ACACGTCCGT
    ATGCTGAGTG TATTGCAGAC AGTGTTTTCT CGTCCACTTG AGAAGCAAGA GTATATGACT
    GCAAATGAGG TGGCCACCAT CTTCTCCAGC CTGGATGACA TCATAGAGAT GCACTATGTT
    TTCTATGAGA ACCTGAAGAA ACTGCGTGAA GAAGATGAAT ACATTGTCAA AACTATCAGC
    ACACCACTTC TCAACAGATT CAGTGGATCT GAAGGAGAAT GGTTTCAAAA GCTCACAGCA
    CGCTTCTGTA GCTACCAGTC CTGGGCTCTG GAGCAGATCA AGAGCAGGCA GAAGAAGGAC
    CCCCGATTCA ATGCTTTTAT ACTGGAAGCA GAGAGTAAGC CACAGTGTCG GAGGCTACAG
    CTAAAGGATA TCATACCCAT AGAGATGCAG AGACTAACAA AATACCCTCT TCTGCTGGAA
    AACATTGCTA AGAACACAGA GGATGCAACA GAAAAAGAGA AAATTAACCA AGCAGCAGAA
    TCCTGCCGTA AGATCCTCAA CCATGTCAAT GAAGAAGTCA AACAAATGGA GAACCTGCTG
    ATTTTGAAAG ACTACCAGCG TCGACTGGAT ACCTCAGGAC TGAAACCCAG CAATGAACTC
    TACAGTGAGT ACAAGACCAT TGATCTGACC AACCGAACGA TGCTGTTTGA AGGACCTCTG
    ACATGGAGGG TGACCAAAGA GAAAGCAATC GATGTGCACT GTGTGTTGCT GTCAGACCTG
    CTGGTCTTGT TTCAGAAGCA GGATGATAAG ATGCTTTTGA AGTGTCAGAG CAAAAGCAAC
    ATCGCCGTTC AGGAAGGCAA GCAGATGCTG AGTCCAATCA TCAAGCTGGA GTCTGTTTTC
    CTCAGAGAGG TGGCCACTGA CCCGAAGGCC CTGTATGTTA TCTTCACCTG GGACAGTGGA
    GCACAGATCT ATGAACTGGT GGCTCAGTCT GTCGGGGAGA GGAAAAACTG GACAGAGGCG
    ATTAAGTCTG CGGTTGATGA GCTGAAGAAG GCATCAAAGA GCAACACCAA ACTGGAAAGG
    AATAGCAGGG CTGGCAGTGT GCCAAACAAC ATTGCTGTAC CTCTATATGG CCCAGCTCAT
    CCTCCTCTTA GCCCCACTGA AAATGGAGGT GAACTGCTGA AAAATCGCGT CGGGCGATTT
    AGTGAGAAGA TTCGAGAAAG TGATCCTCCA CTCATCGACT ATCTAGCAGC CAACGGCTTT
    GACCTGCTCA CCCACAGCCA CACACCTCCG GAGAAATCTG CTACTGTTGC TTTGGATGAA
    GTCATGTGTT TGAAGAGGCT ATTGGTTGGG AGTATTAGCC TGTCAGATGA TGCTCAGACT
    CTTGGGGAAA ACGGTGGGAT GGCACCTGAA AGTCAGGATG CAGATGGAAC TCAACTGTCA
    GTGGAGGAAT CGAGCACAGA AGCAGGTCCT GAAGTAGAAG AGCATGAGGA AGATGTAGAG
    AATAAAGGAG AAGAGGAGAA AGGTATCAGT GCTCCTCTCG TGCTGTCTGA GGAGCGCATG
    GAGGAGGTGC AGATGAGACT GCAGGCTCTG GAGGAGCAGC TGAAGAGATT GAGGTTGGTT
    GAGGAGGACC ATTATAAATT ACAAGAAGCT CTTGCTAAAT ACACACTTCA GGGGGGCCAT
    TACAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322381.1
    CDS373..2961
    Translation

    Target ORF information:

    RefSeq Version XM_021466706.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 1b (arhgef1b), transcript variant X10, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466706.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    ATGTTACATA ATTGTAAACG CCCCTCAATT GTTCCTGACA AGGACAAAAG TAACTCCATC 
    TTTGGAGCAG TGGCTTATTA TATGAAGTAT CTTGGAGTCA AGACCAGAGC TTTTGACAAC
    AAGAAGTCCA AGTCAGGATG GCGGCCCTCG GTTCCATCTA TGCTGAAGCC ATGGTCAGTC
    AACAAGCAGA TTAAACCAGT CAAACCGCCT GGAATCGGCT ATGACGGTAA GCAAAGTAGA
    TTGGACTTTG AAGCAAAGCA AAACAAACAA CCTCCTCATC GAGTGTCTGT TAGTGATAGT
    GTCACCCCTC CAGGGCGAAA AACTGGTATT ACCGTGTCAG GGAGCACTCA TGGGTCAGAG
    AGCAGTGAAG AGTTACCCAT CAGTGTGAGT GTGACTCCCA GCCCTGACTT TCAAACTGAT
    ACAGTAGGTA TCATCAACAA TCGCACAGAC CCCAAACCAG CCTCTGAGGG TGGAGAAGTG
    TCTCCAGTCA GCACTGGTGG GACTGTGTCC ATCTGTGAAC CTCTCGCTGT CATTGATGTC
    CCTCCAGAAG ACATTCAGGA CAGCGGCAGA AAGAAGCCAA GGAATGTGCG TCGCAGTGAA
    AGCCTTTGTG TGGAGCGGCG TCACTCTCGT CGTGGTGGCT CTACTCGGGC CAAGCAGTCT
    CGTTCCCGCA GCGACGTGGA CCTCCAGTCA ACTTCCACAT CCCATCCCCT CTCACCCTCC
    CCACAGCATT CAGTCTCCGT GGAAGGTGAC AAAACACCGG AAATCGCTCT CCTCACTCCC
    ACTTTTGTCC CGGCCCCTCA GCAAGAAGAG ATAGAGCCCC GTCTCCTGGA GCTCGAGCAG
    GACCCACTCA ACTGGAGAGA ACTTGCTTCC AGTGAAAGCC TGCATAAGCT GAGCAAGAAG
    GAAGCCAAAA GACAAGAAGT CATAAATGAG CTGTTTGTGA CAGAACAAGC ACACGTCCGT
    ATGCTGAGTG TATTGCAGAC AGTGTTTTCT CGTCCACTTG AGAAGCAAGA GTATATGACT
    GCAAATGAGG TGGCCACCAT CTTCTCCAGC CTGGATGACA TCATAGAGAT GCACTATGTT
    TTCTATGAGA ACCTGAAGAA ACTGCGTGAA GAAGATGAAT ACATTGTCAA AACTATCAGC
    ACACCACTTC TCAACAGATT CAGTGGATCT GAAGGAGAAT GGTTTCAAAA GCTCACAGCA
    CGCTTCTGTA GCTACCAGTC CTGGGCTCTG GAGCAGATCA AGAGCAGGCA GAAGAAGGAC
    CCCCGATTCA ATGCTTTTAT ACTGGAAGCA GAGAGTAAGC CACAGTGTCG GAGGCTACAG
    CTAAAGGATA TCATACCCAT AGAGATGCAG AGACTAACAA AATACCCTCT TCTGCTGGAA
    AACATTGCTA AGAACACAGA GGATGCAACA GAAAAAGAGA AAATTAACCA AGCAGCAGAA
    TCCTGCCGTA AGATCCTCAA CCATGTCAAT GAAGAAGTCA AACAAATGGA GAACCTGCTG
    ATTTTGAAAG ACTACCAGCG TCGACTGGAT ACCTCAGGAC TGAAACCCAG CAATGAACTC
    TACAGTGAGT ACAAGACCAT TGATCTGACC AACCGAACGA TGCTGTTTGA AGGACCTCTG
    ACATGGAGGG TGACCAAAGA GAAAGCAATC GATGTGCACT GTGTGTTGCT GTCAGACCTG
    CTGGTCTTGT TTCAGAAGCA GGATGATAAG ATGCTTTTGA AGTGTCAGAG CAAAAGCAAC
    ATCGCCGTTC AGGAAGGCAA GCAGATGCTG AGTCCAATCA TCAAGCTGGA GTCTGTTTTC
    CTCAGAGAGG TGGCCACTGA CCCGAAGGCC CTGTATGTTA TCTTCACCTG GGACAGTGGA
    GCACAGATCT ATGAACTGGT GGCTCAGTCT GTCGGGGAGA GGAAAAACTG GACAGAGGCG
    ATTAAGTCTG CGGTTGATGA GCTGAAGAAG GCATCAAAGA GCAACACCAA ACTGGAAAGG
    AATAGCAGGG CTGGCAGTGT GCCAAACAAC ATTGCTGTAC CTCTATATGG CCCAGCTCAT
    CCTCCTCTTA GCCCCACTGA AAATGGAGGT GAACTGCTGA AAAATCGCGT CGGGCGATTT
    AGTGAGAAGA TTCGAGAAAG TGATCCTCCA CTCATCGACT ATCTAGCAGC CAACGGCTTT
    GACCTGCTCA CCCACAGCCA CACACCTCCG GAGAAATCTG CTACTGTTGC TTTGGATGAA
    GTCATGTGTT TGAAGAGGCT ATTGGTTGGG AGTATTAGCC TGTCAGATGA TGCTCAGACT
    CTTGGGGAAA ACGGTGGGAT GGCACCTGAA AGTCAGGATG CAGATGGAAC TCAACTGTCA
    GTGGAGGAAT CGAGCACAGA AGCAGGTCCT GAAGTAGAAG AGCATGAGGA AGATGTAGAG
    AATAAAGGAG AAGAGGAGAA AGGTATCAGT GCTCCTCTCG TGCTGTCTGA GGAGCGCATG
    GAGGAGGTGC AGATGAGACT GCAGGCTCTG GAGGAGCAGC TGAAGAGATT GAGGTTGGTT
    GAGGAGGACC ATTATAAATT ACAAGAAGCT CTTGCTAAAT ACACACTTCA GGGGGGCCAT
    TACAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149241
    Clone ID Related Accession (Same CDS sequence) XM_021466698.1
    Accession Version XM_021466698.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3249bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 1 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGTTGT TCTCCATTCT TGTAGGAGCT CGGTTTGCTG GTGTTGGTAG GACCTGCGAC 
    AGCATGGATT ACGAAGACGC CCATAATGGG CGCGGGCTGT CAGGTGCTCA GGGTTTAAAT
    CCAGTCATGA ACATAATCGG AGCAGAGGAT GAAGACTTTG AGAATGATGA GCAGCCTATG
    GTGGACGATC AGTGCATTCA CTTTAACAGT ATTGAGCTGT TGAAGTCCAG GCCCACCCAC
    CTGCTGGTGT TCCTTCATCA TGTCATGCTA CAGTTTGACT GTGCTCCTGT GCTATGCTAC
    CTTCATGCTG ACCTCATGAA GAACCTCAAT ACCAAAGACA CCAAGAAGCA CTTTGGGGAA
    TTCTACAACA CTTTCCTGGA GAAAGGAGCG ATTCTCAAAG TATCTGTACC AAGTAACATC
    GCCCCTGAAA TAGATCGCTT GCGTCCAGAA CTGATCAATG AAGAGCAGCA AAAACGTTAC
    GCTAATGAAA TACAGTTTGC GCAGAGTCCA GAGATACTGC GACAGCTGGA AGACTTCAGG
    CAAAAGAGGA TGATGGGAAT GACACCTAAT GAGCGGGAAT TAAATGATGT GGAATCCCAT
    CGACCCACTG ATCGCATCCC TATGGACATG AAGGAGAAGG CTGTCGCTGA ATCCCTGCTG
    GAAAAGATGG TTGAGGCCAA CCCCTCAATT GTTCCTGACA AGGACAAAAG TAACTCCATC
    TTTGGAGCAG TGGCTTATTA TATGAAGTAT CTTGGAGTCA AGACCAGAGC TTTTGACAAC
    AAGAAGTCCA AGTCAGGATG GCGGCCCTCG GTTCCATCTA TGCTGAAGCC ATGGTCAGTC
    AACAAGCAGA TTAAACCAGT CAAACCGCCT GGAATCGGCT ATGACGGTAA GCAAAGTAGA
    TTGGACTTTG AAGCAAAGCA AAACAAACAA CCTCCTCATC GAGTGTCTGT TAGTGATAGT
    GTCACCCCTC CAGGGCGAAA AACTGGTATT ACCGTGTCAG GGAGCACTCA TGGGTCAGAG
    AGCAGTGAAG AGTTACCCAT CAGTGTGAGT GTGACTCCCA GCCCTGACTT TCAAACTGAT
    ACAGTAGGTA TCATCAACAA TCGCACAGAC CCCAAACCAG CCTCTGAGGG TGGAGAAGTG
    TCTCCAGTCA GCACTGGTGG GACTGTGTCC ATCTGTGAAC CTCTCGCTGT CATTGATGTC
    CCTCCAGAAG ACATTCAGGA CAGCGGCAGA AAGAAGCCAA GGAATGTGCG TCGCAGTGAA
    AGCCTTTGTG TGGAGCGGCG TCACTCTCGT CGTGGTGGCT CTACTCGGGC CAAGCAGTCT
    CGTTCCCGCA GCGACGTGGA CCTCCAGTCA ACTTCCACAT CCCATCCCCT CTCACCCTCC
    CCACAGCATT CAGTCTCCGT GGAAGGTGAC AAAACACCGG AAATCGCTCT CCTCACTCCC
    ACTTTTGTCC CGGCCCCTCA GCAAGAAGAG ATAGAGCCCC GTCTCCTGGA GCTCGAGCAG
    GACCCACTCA ACTGGAGAGA ACTTGCTTCC AGTGAAAGCC TGCATAAGCT GAGCAAGAAG
    GAAGCCAAAA GACAAGAAGT CATAAATGAG CTGTTTGTGA CAGAACAAGC ACACGTCCGT
    ATGCTGAGTG TATTGCAGAC AGTGTTTTCT CGTCCACTTG AGAAGCAAGA GTATATGACT
    GCAAATGAGG TGGCCACCAT CTTCTCCAGC CTGGATGACA TCATAGAGAT GCACTATGTT
    TTCTATGAGA ACCTGAAGAA ACTGCGTGAA GAAGATGAAT ACATTGTCAA AACTATCAGC
    ACACCACTTC TCAACAGATT CAGTGGATCT GAAGGAGAAT GGTTTCAAAA GCTCACAGCA
    CGCTTCTGTA GCTACCAGTC CTGGGCTCTG GAGCAGATCA AGAGCAGGCA GAAGAAGGAC
    CCCCGATTCA ATGCTTTTAT ACTGGAAGCA GAGAGTAAGC CACAGTGTCG GAGGCTACAG
    CTAAAGGATA TCATACCCAT AGAGATGCAG AGACTAACAA AATACCCTCT TCTGCTGGAA
    AACATTGCTA AGAACACAGA GGATGCAACA GAAAAAGAGA AAATTAACCA AGCAGCAGAA
    TCCTGCCGTA AGATCCTCAA CCATGTCAAT GAAGAAGTCA AACAAATGGA GAACCTGCTG
    ATTTTGAAAG ACTACCAGCG TCGACTGGAT ACCTCAGGAC TGAAACCCAG CAATGAACTC
    TACAGTGAGT ACAAGACCAT TGATCTGACC AACCGAACGA TGCTGTTTGA AGGACCTCTG
    ACATGGAGGG TGACCAAAGA GAAAGCAATC GATGTGCACT GTGTGTTGCT GTCAGACCTG
    CTGGTCTTGT TTCAGAAGCA GGATGATAAG ATGCTTTTGA AGTGTCAGAG CAAAAGCAAC
    ATCGCCGTTC AGGAAGGCAA GCAGATGCTG AGTCCAATCA TCAAGCTGGA GTCTGTTTTC
    CTCAGAGAGG TGGCCACTGA CCCGAAGGCC CTGTATGTTA TCTTCACCTG GGACAGTGGA
    GCACAGATCT ATGAACTGGT GGCTCAGTCT GTCGGGGAGA GGAAAAACTG GACAGAGGCG
    ATTAAGTCTG CGGTTGATGA GCTGAAGAAG GCATCAAAGA GCAACACCAA ACTGGAAAGG
    AATAGCAGGG CTGGCAGTGT GCCAAACAAC ATTGCTGTAC CTCTATATGG CCCAGCTCAT
    CCTCCTCTTA GCCCCACTGA AAATGGAGGT GAACTGCTGA AAAATCGCGT CGGGCGATTT
    AGTGAGAAGA TTCGAGAAAG TGATCCTCCA CTCATCGACT ATCTAGCAGC CAACGGCTTT
    GACCTGCTCA CCCACAGCCA CACACCTCCG GAGAAATCTG CTACTGTTGC TTTGGATGAA
    GTCATGTGTT TGAAGAGGCT ATTGGTTGGG AGTATTAGCC TGTCAGATGA TGCTCAGACT
    CTTGGGGAAA ACGGTGGGAT GGCACCTGAA AGTCAGGATG CAGATGGAAC TCAACTGTCA
    GTGGAGGAAT CGAGCACAGA AGCAGGTCCT GAAGTAGAAG AGCATGAGGA AGATGTAGAG
    AATAAAGGAG AAGAGGAGAA AGGTATCAGT GCTCCTCTCG TGCTGTCTGA GGAGCGCATG
    GAGGAGGTGC AGATGAGACT GCAGGCTCTG GAGGAGCAGC TGAAGAGATT GAGGTTGGTT
    GAGGAGGACC ATTATAAATT ACAAGAAGCT CTTGCTAAAT ACACACTTCA GGGGGGCCAT
    TACAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322373.1
    CDS263..3511
    Translation

    Target ORF information:

    RefSeq Version XM_021466698.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 1b (arhgef1b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466698.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGTTGT TCTCCATTCT TGTAGGAGCT CGGTTTGCTG GTGTTGGTAG GACCTGCGAC 
    AGCATGGATT ACGAAGACGC CCATAATGGG CGCGGGCTGT CAGGTGCTCA GGGTTTAAAT
    CCAGTCATGA ACATAATCGG AGCAGAGGAT GAAGACTTTG AGAATGATGA GCAGCCTATG
    GTGGACGATC AGTGCATTCA CTTTAACAGT ATTGAGCTGT TGAAGTCCAG GCCCACCCAC
    CTGCTGGTGT TCCTTCATCA TGTCATGCTA CAGTTTGACT GTGCTCCTGT GCTATGCTAC
    CTTCATGCTG ACCTCATGAA GAACCTCAAT ACCAAAGACA CCAAGAAGCA CTTTGGGGAA
    TTCTACAACA CTTTCCTGGA GAAAGGAGCG ATTCTCAAAG TATCTGTACC AAGTAACATC
    GCCCCTGAAA TAGATCGCTT GCGTCCAGAA CTGATCAATG AAGAGCAGCA AAAACGTTAC
    GCTAATGAAA TACAGTTTGC GCAGAGTCCA GAGATACTGC GACAGCTGGA AGACTTCAGG
    CAAAAGAGGA TGATGGGAAT GACACCTAAT GAGCGGGAAT TAAATGATGT GGAATCCCAT
    CGACCCACTG ATCGCATCCC TATGGACATG AAGGAGAAGG CTGTCGCTGA ATCCCTGCTG
    GAAAAGATGG TTGAGGCCAA CCCCTCAATT GTTCCTGACA AGGACAAAAG TAACTCCATC
    TTTGGAGCAG TGGCTTATTA TATGAAGTAT CTTGGAGTCA AGACCAGAGC TTTTGACAAC
    AAGAAGTCCA AGTCAGGATG GCGGCCCTCG GTTCCATCTA TGCTGAAGCC ATGGTCAGTC
    AACAAGCAGA TTAAACCAGT CAAACCGCCT GGAATCGGCT ATGACGGTAA GCAAAGTAGA
    TTGGACTTTG AAGCAAAGCA AAACAAACAA CCTCCTCATC GAGTGTCTGT TAGTGATAGT
    GTCACCCCTC CAGGGCGAAA AACTGGTATT ACCGTGTCAG GGAGCACTCA TGGGTCAGAG
    AGCAGTGAAG AGTTACCCAT CAGTGTGAGT GTGACTCCCA GCCCTGACTT TCAAACTGAT
    ACAGTAGGTA TCATCAACAA TCGCACAGAC CCCAAACCAG CCTCTGAGGG TGGAGAAGTG
    TCTCCAGTCA GCACTGGTGG GACTGTGTCC ATCTGTGAAC CTCTCGCTGT CATTGATGTC
    CCTCCAGAAG ACATTCAGGA CAGCGGCAGA AAGAAGCCAA GGAATGTGCG TCGCAGTGAA
    AGCCTTTGTG TGGAGCGGCG TCACTCTCGT CGTGGTGGCT CTACTCGGGC CAAGCAGTCT
    CGTTCCCGCA GCGACGTGGA CCTCCAGTCA ACTTCCACAT CCCATCCCCT CTCACCCTCC
    CCACAGCATT CAGTCTCCGT GGAAGGTGAC AAAACACCGG AAATCGCTCT CCTCACTCCC
    ACTTTTGTCC CGGCCCCTCA GCAAGAAGAG ATAGAGCCCC GTCTCCTGGA GCTCGAGCAG
    GACCCACTCA ACTGGAGAGA ACTTGCTTCC AGTGAAAGCC TGCATAAGCT GAGCAAGAAG
    GAAGCCAAAA GACAAGAAGT CATAAATGAG CTGTTTGTGA CAGAACAAGC ACACGTCCGT
    ATGCTGAGTG TATTGCAGAC AGTGTTTTCT CGTCCACTTG AGAAGCAAGA GTATATGACT
    GCAAATGAGG TGGCCACCAT CTTCTCCAGC CTGGATGACA TCATAGAGAT GCACTATGTT
    TTCTATGAGA ACCTGAAGAA ACTGCGTGAA GAAGATGAAT ACATTGTCAA AACTATCAGC
    ACACCACTTC TCAACAGATT CAGTGGATCT GAAGGAGAAT GGTTTCAAAA GCTCACAGCA
    CGCTTCTGTA GCTACCAGTC CTGGGCTCTG GAGCAGATCA AGAGCAGGCA GAAGAAGGAC
    CCCCGATTCA ATGCTTTTAT ACTGGAAGCA GAGAGTAAGC CACAGTGTCG GAGGCTACAG
    CTAAAGGATA TCATACCCAT AGAGATGCAG AGACTAACAA AATACCCTCT TCTGCTGGAA
    AACATTGCTA AGAACACAGA GGATGCAACA GAAAAAGAGA AAATTAACCA AGCAGCAGAA
    TCCTGCCGTA AGATCCTCAA CCATGTCAAT GAAGAAGTCA AACAAATGGA GAACCTGCTG
    ATTTTGAAAG ACTACCAGCG TCGACTGGAT ACCTCAGGAC TGAAACCCAG CAATGAACTC
    TACAGTGAGT ACAAGACCAT TGATCTGACC AACCGAACGA TGCTGTTTGA AGGACCTCTG
    ACATGGAGGG TGACCAAAGA GAAAGCAATC GATGTGCACT GTGTGTTGCT GTCAGACCTG
    CTGGTCTTGT TTCAGAAGCA GGATGATAAG ATGCTTTTGA AGTGTCAGAG CAAAAGCAAC
    ATCGCCGTTC AGGAAGGCAA GCAGATGCTG AGTCCAATCA TCAAGCTGGA GTCTGTTTTC
    CTCAGAGAGG TGGCCACTGA CCCGAAGGCC CTGTATGTTA TCTTCACCTG GGACAGTGGA
    GCACAGATCT ATGAACTGGT GGCTCAGTCT GTCGGGGAGA GGAAAAACTG GACAGAGGCG
    ATTAAGTCTG CGGTTGATGA GCTGAAGAAG GCATCAAAGA GCAACACCAA ACTGGAAAGG
    AATAGCAGGG CTGGCAGTGT GCCAAACAAC ATTGCTGTAC CTCTATATGG CCCAGCTCAT
    CCTCCTCTTA GCCCCACTGA AAATGGAGGT GAACTGCTGA AAAATCGCGT CGGGCGATTT
    AGTGAGAAGA TTCGAGAAAG TGATCCTCCA CTCATCGACT ATCTAGCAGC CAACGGCTTT
    GACCTGCTCA CCCACAGCCA CACACCTCCG GAGAAATCTG CTACTGTTGC TTTGGATGAA
    GTCATGTGTT TGAAGAGGCT ATTGGTTGGG AGTATTAGCC TGTCAGATGA TGCTCAGACT
    CTTGGGGAAA ACGGTGGGAT GGCACCTGAA AGTCAGGATG CAGATGGAAC TCAACTGTCA
    GTGGAGGAAT CGAGCACAGA AGCAGGTCCT GAAGTAGAAG AGCATGAGGA AGATGTAGAG
    AATAAAGGAG AAGAGGAGAA AGGTATCAGT GCTCCTCTCG TGCTGTCTGA GGAGCGCATG
    GAGGAGGTGC AGATGAGACT GCAGGCTCTG GAGGAGCAGC TGAAGAGATT GAGGTTGGTT
    GAGGAGGACC ATTATAAATT ACAAGAAGCT CTTGCTAAAT ACACACTTCA GGGGGGCCAT
    TACAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149242
    Clone ID Related Accession (Same CDS sequence) XM_021466699.1
    Accession Version XM_021466699.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3246bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 1 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGTTGT TCTCCATTCT TGTAGGAGCT CGGTTTGCTG GTGTTGGTAG GACCTGCGAC 
    AGCATGGATT ACGAAGACGC CCATAATGGG CGCGGGCTGT CAGGTGCTCA GGGTTTAAAT
    CCAGTCATGA ACATAATCGG AGCAGAGGAT GAAGACTTTG AGAATGATGA GCAGCCTATG
    GTGGACGATC AGTGCATTCA CTTTAACAGT ATTGAGCTGT TGAAGTCCAG GCCCACCCAC
    CTGCTGGTGT TCCTTCATCA TGTCATGCTA CAGTTTGACT GTGCTCCTGT GCTATGCTAC
    CTTCATGCTG ACCTCATGAA GAACCTCAAT ACCAAAGACA CCAAGAAGCA CTTTGGGGAA
    TTCTACAACA CTTTCCTGGA GAAAGGAGCG ATTCTCAAAG TATCTGTACC AAGTAACATC
    GCCCCTGAAA TAGATCGCTT GCGTCCAGAA CTGATCAATG AAGAGCAGCA AAAACGTTAC
    GCTAATGAAA TACAGTTTGC GCAGAGTCCA GAGATACTGC GACAGCTGGA AGACTTCAGG
    CAAAAGAGGA TGATGGGAAT GACACCTAAT GAGCGGGAAT TAAATGATGT GGAATCCCAT
    CGACCCACTG ATCGCATCCC TATGGACATG AAGGAGAAGG CTGTCGCTGA ATCCCTGCTG
    GAAAAGATGG TTGAGGCCAA CCCCTCAATT GTTCCTGACA AGGACAAAAG TAACTCCATC
    TTTGGAGCAG TGGCTTATTA TATGAAGTAT CTTGGAGTCA AGACCAGAGC TTTTGACAAC
    AAGAAGTCCA AGTCAGGATG GCGGCCCTCG GTTCCATCTA TGCTGAAGCC ATGGTCAGTC
    AACAAGCAGA TTAAACCAGT CAAACCGCCT GGAATCGGCT ATGACGGTAA GCAAAGTAGA
    TTGGACTTTG AAGCAAAGCA AAACAAACAA CCTCCTCATC GAGTGTCTGT TAGTGATAGT
    GTCACCCCTC CAGGGCGAAA AACTGGTATT ACCGTGTCAG GGAGCACTCA TGGGTCAGAG
    AGCAGTGAAG AGTTACCCAT CAGTGTGAGT GTGACTCCCA GCCCTGACTT TCAAACTGAT
    ACAGGTATCA TCAACAATCG CACAGACCCC AAACCAGCCT CTGAGGGTGG AGAAGTGTCT
    CCAGTCAGCA CTGGTGGGAC TGTGTCCATC TGTGAACCTC TCGCTGTCAT TGATGTCCCT
    CCAGAAGACA TTCAGGACAG CGGCAGAAAG AAGCCAAGGA ATGTGCGTCG CAGTGAAAGC
    CTTTGTGTGG AGCGGCGTCA CTCTCGTCGT GGTGGCTCTA CTCGGGCCAA GCAGTCTCGT
    TCCCGCAGCG ACGTGGACCT CCAGTCAACT TCCACATCCC ATCCCCTCTC ACCCTCCCCA
    CAGCATTCAG TCTCCGTGGA AGGTGACAAA ACACCGGAAA TCGCTCTCCT CACTCCCACT
    TTTGTCCCGG CCCCTCAGCA AGAAGAGATA GAGCCCCGTC TCCTGGAGCT CGAGCAGGAC
    CCACTCAACT GGAGAGAACT TGCTTCCAGT GAAAGCCTGC ATAAGCTGAG CAAGAAGGAA
    GCCAAAAGAC AAGAAGTCAT AAATGAGCTG TTTGTGACAG AACAAGCACA CGTCCGTATG
    CTGAGTGTAT TGCAGACAGT GTTTTCTCGT CCACTTGAGA AGCAAGAGTA TATGACTGCA
    AATGAGGTGG CCACCATCTT CTCCAGCCTG GATGACATCA TAGAGATGCA CTATGTTTTC
    TATGAGAACC TGAAGAAACT GCGTGAAGAA GATGAATACA TTGTCAAAAC TATCAGCACA
    CCACTTCTCA ACAGATTCAG TGGATCTGAA GGAGAATGGT TTCAAAAGCT CACAGCACGC
    TTCTGTAGCT ACCAGTCCTG GGCTCTGGAG CAGATCAAGA GCAGGCAGAA GAAGGACCCC
    CGATTCAATG CTTTTATACT GGAAGCAGAG AGTAAGCCAC AGTGTCGGAG GCTACAGCTA
    AAGGATATCA TACCCATAGA GATGCAGAGA CTAACAAAAT ACCCTCTTCT GCTGGAAAAC
    ATTGCTAAGA ACACAGAGGA TGCAACAGAA AAAGAGAAAA TTAACCAAGC AGCAGAATCC
    TGCCGTAAGA TCCTCAACCA TGTCAATGAA GAAGTCAAAC AAATGGAGAA CCTGCTGATT
    TTGAAAGACT ACCAGCGTCG ACTGGATACC TCAGGACTGA AACCCAGCAA TGAACTCTAC
    AGTGAGTACA AGACCATTGA TCTGACCAAC CGAACGATGC TGTTTGAAGG ACCTCTGACA
    TGGAGGGTGA CCAAAGAGAA AGCAATCGAT GTGCACTGTG TGTTGCTGTC AGACCTGCTG
    GTCTTGTTTC AGAAGCAGGA TGATAAGATG CTTTTGAAGT GTCAGAGCAA AAGCAACATC
    GCCGTTCAGG AAGGCAAGCA GATGCTGAGT CCAATCATCA AGCTGGAGTC TGTTTTCCTC
    AGAGAGGTGG CCACTGACCC GAAGGCCCTG TATGTTATCT TCACCTGGGA CAGTGGAGCA
    CAGATCTATG AACTGGTGGC TCAGTCTGTC GGGGAGAGGA AAAACTGGAC AGAGGCGATT
    AAGTCTGCGG TTGATGAGCT GAAGAAGGCA TCAAAGAGCA ACACCAAACT GGAAAGGAAT
    AGCAGGGCTG GCAGTGTGCC AAACAACATT GCTGTACCTC TATATGGCCC AGCTCATCCT
    CCTCTTAGCC CCACTGAAAA TGGAGGTGAA CTGCTGAAAA ATCGCGTCGG GCGATTTAGT
    GAGAAGATTC GAGAAAGTGA TCCTCCACTC ATCGACTATC TAGCAGCCAA CGGCTTTGAC
    CTGCTCACCC ACAGCCACAC ACCTCCGGAG AAATCTGCTA CTGTTGCTTT GGATGAAGTC
    ATGTGTTTGA AGAGGCTATT GGTTGGGAGT ATTAGCCTGT CAGATGATGC TCAGACTCTT
    GGGGAAAACG GTGGGATGGC ACCTGAAAGT CAGGATGCAG ATGGAACTCA ACTGTCAGTG
    GAGGAATCGA GCACAGAAGC AGGTCCTGAA GTAGAAGAGC ATGAGGAAGA TGTAGAGAAT
    AAAGGAGAAG AGGAGAAAGG TATCAGTGCT CCTCTCGTGC TGTCTGAGGA GCGCATGGAG
    GAGGTGCAGA TGAGACTGCA GGCTCTGGAG GAGCAGCTGA AGAGATTGAG GTTGGTTGAG
    GAGGACCATT ATAAATTACA AGAAGCTCTT GCTAAATACA CACTTCAGGG GGGCCATTAC
    AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322374.1
    CDS264..3509
    Translation

    Target ORF information:

    RefSeq Version XM_021466699.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 1b (arhgef1b), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466699.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    ATGCTGTTGT TCTCCATTCT TGTAGGAGCT CGGTTTGCTG GTGTTGGTAG GACCTGCGAC 
    AGCATGGATT ACGAAGACGC CCATAATGGG CGCGGGCTGT CAGGTGCTCA GGGTTTAAAT
    CCAGTCATGA ACATAATCGG AGCAGAGGAT GAAGACTTTG AGAATGATGA GCAGCCTATG
    GTGGACGATC AGTGCATTCA CTTTAACAGT ATTGAGCTGT TGAAGTCCAG GCCCACCCAC
    CTGCTGGTGT TCCTTCATCA TGTCATGCTA CAGTTTGACT GTGCTCCTGT GCTATGCTAC
    CTTCATGCTG ACCTCATGAA GAACCTCAAT ACCAAAGACA CCAAGAAGCA CTTTGGGGAA
    TTCTACAACA CTTTCCTGGA GAAAGGAGCG ATTCTCAAAG TATCTGTACC AAGTAACATC
    GCCCCTGAAA TAGATCGCTT GCGTCCAGAA CTGATCAATG AAGAGCAGCA AAAACGTTAC
    GCTAATGAAA TACAGTTTGC GCAGAGTCCA GAGATACTGC GACAGCTGGA AGACTTCAGG
    CAAAAGAGGA TGATGGGAAT GACACCTAAT GAGCGGGAAT TAAATGATGT GGAATCCCAT
    CGACCCACTG ATCGCATCCC TATGGACATG AAGGAGAAGG CTGTCGCTGA ATCCCTGCTG
    GAAAAGATGG TTGAGGCCAA CCCCTCAATT GTTCCTGACA AGGACAAAAG TAACTCCATC
    TTTGGAGCAG TGGCTTATTA TATGAAGTAT CTTGGAGTCA AGACCAGAGC TTTTGACAAC
    AAGAAGTCCA AGTCAGGATG GCGGCCCTCG GTTCCATCTA TGCTGAAGCC ATGGTCAGTC
    AACAAGCAGA TTAAACCAGT CAAACCGCCT GGAATCGGCT ATGACGGTAA GCAAAGTAGA
    TTGGACTTTG AAGCAAAGCA AAACAAACAA CCTCCTCATC GAGTGTCTGT TAGTGATAGT
    GTCACCCCTC CAGGGCGAAA AACTGGTATT ACCGTGTCAG GGAGCACTCA TGGGTCAGAG
    AGCAGTGAAG AGTTACCCAT CAGTGTGAGT GTGACTCCCA GCCCTGACTT TCAAACTGAT
    ACAGGTATCA TCAACAATCG CACAGACCCC AAACCAGCCT CTGAGGGTGG AGAAGTGTCT
    CCAGTCAGCA CTGGTGGGAC TGTGTCCATC TGTGAACCTC TCGCTGTCAT TGATGTCCCT
    CCAGAAGACA TTCAGGACAG CGGCAGAAAG AAGCCAAGGA ATGTGCGTCG CAGTGAAAGC
    CTTTGTGTGG AGCGGCGTCA CTCTCGTCGT GGTGGCTCTA CTCGGGCCAA GCAGTCTCGT
    TCCCGCAGCG ACGTGGACCT CCAGTCAACT TCCACATCCC ATCCCCTCTC ACCCTCCCCA
    CAGCATTCAG TCTCCGTGGA AGGTGACAAA ACACCGGAAA TCGCTCTCCT CACTCCCACT
    TTTGTCCCGG CCCCTCAGCA AGAAGAGATA GAGCCCCGTC TCCTGGAGCT CGAGCAGGAC
    CCACTCAACT GGAGAGAACT TGCTTCCAGT GAAAGCCTGC ATAAGCTGAG CAAGAAGGAA
    GCCAAAAGAC AAGAAGTCAT AAATGAGCTG TTTGTGACAG AACAAGCACA CGTCCGTATG
    CTGAGTGTAT TGCAGACAGT GTTTTCTCGT CCACTTGAGA AGCAAGAGTA TATGACTGCA
    AATGAGGTGG CCACCATCTT CTCCAGCCTG GATGACATCA TAGAGATGCA CTATGTTTTC
    TATGAGAACC TGAAGAAACT GCGTGAAGAA GATGAATACA TTGTCAAAAC TATCAGCACA
    CCACTTCTCA ACAGATTCAG TGGATCTGAA GGAGAATGGT TTCAAAAGCT CACAGCACGC
    TTCTGTAGCT ACCAGTCCTG GGCTCTGGAG CAGATCAAGA GCAGGCAGAA GAAGGACCCC
    CGATTCAATG CTTTTATACT GGAAGCAGAG AGTAAGCCAC AGTGTCGGAG GCTACAGCTA
    AAGGATATCA TACCCATAGA GATGCAGAGA CTAACAAAAT ACCCTCTTCT GCTGGAAAAC
    ATTGCTAAGA ACACAGAGGA TGCAACAGAA AAAGAGAAAA TTAACCAAGC AGCAGAATCC
    TGCCGTAAGA TCCTCAACCA TGTCAATGAA GAAGTCAAAC AAATGGAGAA CCTGCTGATT
    TTGAAAGACT ACCAGCGTCG ACTGGATACC TCAGGACTGA AACCCAGCAA TGAACTCTAC
    AGTGAGTACA AGACCATTGA TCTGACCAAC CGAACGATGC TGTTTGAAGG ACCTCTGACA
    TGGAGGGTGA CCAAAGAGAA AGCAATCGAT GTGCACTGTG TGTTGCTGTC AGACCTGCTG
    GTCTTGTTTC AGAAGCAGGA TGATAAGATG CTTTTGAAGT GTCAGAGCAA AAGCAACATC
    GCCGTTCAGG AAGGCAAGCA GATGCTGAGT CCAATCATCA AGCTGGAGTC TGTTTTCCTC
    AGAGAGGTGG CCACTGACCC GAAGGCCCTG TATGTTATCT TCACCTGGGA CAGTGGAGCA
    CAGATCTATG AACTGGTGGC TCAGTCTGTC GGGGAGAGGA AAAACTGGAC AGAGGCGATT
    AAGTCTGCGG TTGATGAGCT GAAGAAGGCA TCAAAGAGCA ACACCAAACT GGAAAGGAAT
    AGCAGGGCTG GCAGTGTGCC AAACAACATT GCTGTACCTC TATATGGCCC AGCTCATCCT
    CCTCTTAGCC CCACTGAAAA TGGAGGTGAA CTGCTGAAAA ATCGCGTCGG GCGATTTAGT
    GAGAAGATTC GAGAAAGTGA TCCTCCACTC ATCGACTATC TAGCAGCCAA CGGCTTTGAC
    CTGCTCACCC ACAGCCACAC ACCTCCGGAG AAATCTGCTA CTGTTGCTTT GGATGAAGTC
    ATGTGTTTGA AGAGGCTATT GGTTGGGAGT ATTAGCCTGT CAGATGATGC TCAGACTCTT
    GGGGAAAACG GTGGGATGGC ACCTGAAAGT CAGGATGCAG ATGGAACTCA ACTGTCAGTG
    GAGGAATCGA GCACAGAAGC AGGTCCTGAA GTAGAAGAGC ATGAGGAAGA TGTAGAGAAT
    AAAGGAGAAG AGGAGAAAGG TATCAGTGCT CCTCTCGTGC TGTCTGAGGA GCGCATGGAG
    GAGGTGCAGA TGAGACTGCA GGCTCTGGAG GAGCAGCTGA AGAGATTGAG GTTGGTTGAG
    GAGGACCATT ATAAATTACA AGAAGCTCTT GCTAAATACA CACTTCAGGG GGGCCATTAC
    AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149243
    Clone ID Related Accession (Same CDS sequence) XM_021466700.1
    Accession Version XM_021466700.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3237bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 1 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCTGTTGT TCTCCATTCT TGTAGGAGCT CGGTTTGCTG GTGTTGGTAG GACCTGCGAC 
    AGCATGGATT ACGAAGACGC CCATAATGGG CGCGGGCTGT CAGGTGCTCA GGGTTTAAAT
    CCAGTCATGA ACATAATCGG AGCAGAGGAT GAAGACTTTG AGAATGATGA GCAGCCTATG
    GTGGACGATC AGTGCATTCA CTTTAACAGT ATTGAGCTGT TGAAGTCCAG GCCCACCCAC
    CTGCTGGTGT TCCTTCATCA TGTCATGCTA CAGTTTGACT GTGCTCCTGT GCTATGCTAC
    CTTCATGCTG ACCTCATGAA GAACCTCAAT ACCAAAGACA CCAAGAAGCA CTTTGGGGAA
    TTCTACAACA CTTTCCTGGA GAAAGGAGCG ATTCTCAAAG TATCTGTACC AAGTAACATC
    GCCCCTGAAA TAGATCGCTT GCGTCCAGAA CTGATCAATG AAGAGCAGCA AAAACGTTAC
    GCTAATGAAA TACAGTTTGC GCAGAGTCCA GAGATACTGC GACAGCTGGA AGACTTCAGG
    CAAAAGAGGA TGATGGGAAT GACACCTAAT GAGCGGGAAT TAAATGATGT GGAATCCCAT
    CGACCCACTG ATCGCATCCC TATGGACATG AAGGAGAAGG CTGTCGCTGA ATCCCTGCTG
    GAAAAGATGG TTGAGGCCAA CCCCTCAATT GTTCCTGACA AGGACAAAAG TAACTCCATC
    TTTGGAGCAG TGGCTTATTA TATGAAGTAT CTTGGAGTCA AGACCAGAGC TTTTGACAAC
    AAGAAGTCCA AGTCAGGATG GCGGCCCTCG GTTCCATCTA TGCTGAAGCC ATGGTCAGTC
    AACAAGCAGA TTAAACCAGT CAAACCGCCT GGAATCGGCT ATGACGGTAA GCAAAGTAGA
    TTGGACTTTG AAGCAAAGCA AAACAAACAA CCTCCTCATC GAGTGTCTGT TAGTGATAGT
    GTCACCCCTC CAGGGCGAAA AACTGGTATT ACCGTGTCAG GGAGCACTCA TGGGTCAGAG
    AGCAGTGAAG AGTTACCCAT CAGTGTGAGT GTGACTCCCA GCCCTGACTT TCAAACTGAT
    ACAGTAGGTA TCATCAACAA TCGCACAGAC CCCAAACCAG CCTCTGAGGG TGGAGAAGTG
    TCTCCAGTCA GCACTGGTGG GACTGTGTCC ATCTGTGAAC CTCTCGCTGT CATTGATGTC
    CCTCCAGAAG ACATTCAGGA CAGCGGCAGG AATGTGCGTC GCAGTGAAAG CCTTTGTGTG
    GAGCGGCGTC ACTCTCGTCG TGGTGGCTCT ACTCGGGCCA AGCAGTCTCG TTCCCGCAGC
    GACGTGGACC TCCAGTCAAC TTCCACATCC CATCCCCTCT CACCCTCCCC ACAGCATTCA
    GTCTCCGTGG AAGGTGACAA AACACCGGAA ATCGCTCTCC TCACTCCCAC TTTTGTCCCG
    GCCCCTCAGC AAGAAGAGAT AGAGCCCCGT CTCCTGGAGC TCGAGCAGGA CCCACTCAAC
    TGGAGAGAAC TTGCTTCCAG TGAAAGCCTG CATAAGCTGA GCAAGAAGGA AGCCAAAAGA
    CAAGAAGTCA TAAATGAGCT GTTTGTGACA GAACAAGCAC ACGTCCGTAT GCTGAGTGTA
    TTGCAGACAG TGTTTTCTCG TCCACTTGAG AAGCAAGAGT ATATGACTGC AAATGAGGTG
    GCCACCATCT TCTCCAGCCT GGATGACATC ATAGAGATGC ACTATGTTTT CTATGAGAAC
    CTGAAGAAAC TGCGTGAAGA AGATGAATAC ATTGTCAAAA CTATCAGCAC ACCACTTCTC
    AACAGATTCA GTGGATCTGA AGGAGAATGG TTTCAAAAGC TCACAGCACG CTTCTGTAGC
    TACCAGTCCT GGGCTCTGGA GCAGATCAAG AGCAGGCAGA AGAAGGACCC CCGATTCAAT
    GCTTTTATAC TGGAAGCAGA GAGTAAGCCA CAGTGTCGGA GGCTACAGCT AAAGGATATC
    ATACCCATAG AGATGCAGAG ACTAACAAAA TACCCTCTTC TGCTGGAAAA CATTGCTAAG
    AACACAGAGG ATGCAACAGA AAAAGAGAAA ATTAACCAAG CAGCAGAATC CTGCCGTAAG
    ATCCTCAACC ATGTCAATGA AGAAGTCAAA CAAATGGAGA ACCTGCTGAT TTTGAAAGAC
    TACCAGCGTC GACTGGATAC CTCAGGACTG AAACCCAGCA ATGAACTCTA CAGTGAGTAC
    AAGACCATTG ATCTGACCAA CCGAACGATG CTGTTTGAAG GACCTCTGAC ATGGAGGGTG
    ACCAAAGAGA AAGCAATCGA TGTGCACTGT GTGTTGCTGT CAGACCTGCT GGTCTTGTTT
    CAGAAGCAGG ATGATAAGAT GCTTTTGAAG TGTCAGAGCA AAAGCAACAT CGCCGTTCAG
    GAAGGCAAGC AGATGCTGAG TCCAATCATC AAGCTGGAGT CTGTTTTCCT CAGAGAGGTG
    GCCACTGACC CGAAGGCCCT GTATGTTATC TTCACCTGGG ACAGTGGAGC ACAGATCTAT
    GAACTGGTGG CTCAGTCTGT CGGGGAGAGG AAAAACTGGA CAGAGGCGAT TAAGTCTGCG
    GTTGATGAGC TGAAGAAGGC ATCAAAGAGC AACACCAAAC TGGAAAGGAA TAGCAGGGCT
    GGCAGTGTGC CAAACAACAT TGCTGTACCT CTATATGGCC CAGCTCATCC TCCTCTTAGC
    CCCACTGAAA ATGGAGGTGA ACTGCTGAAA AATCGCGTCG GGCGATTTAG TGAGAAGATT
    CGAGAAAGTG ATCCTCCACT CATCGACTAT CTAGCAGCCA ACGGCTTTGA CCTGCTCACC
    CACAGCCACA CACCTCCGGA GAAATCTGCT ACTGTTGCTT TGGATGAAGT CATGTGTTTG
    AAGAGGCTAT TGGTTGGGAG TATTAGCCTG TCAGATGATG CTCAGACTCT TGGGGAAAAC
    GGTGGGATGG CACCTGAAAG TCAGGATGCA GATGGAACTC AACTGTCAGT GGAGGAATCG
    AGCACAGAAG CAGGTCCTGA AGTAGAAGAG CATGAGGAAG ATGTAGAGAA TAAAGGAGAA
    GAGGAGAAAG GTATCAGTGC TCCTCTCGTG CTGTCTGAGG AGCGCATGGA GGAGGTGCAG
    ATGAGACTGC AGGCTCTGGA GGAGCAGCTG AAGAGATTGA GGTTGGTTGA GGAGGACCAT
    TATAAATTAC AAGAAGCTCT TGCTAAATAC ACACTTCAGG GGGGCCATTA CAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322375.1
    CDS263..3499
    Translation

    Target ORF information:

    RefSeq Version XM_021466700.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 1b (arhgef1b), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466700.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCTGTTGT TCTCCATTCT TGTAGGAGCT CGGTTTGCTG GTGTTGGTAG GACCTGCGAC 
    AGCATGGATT ACGAAGACGC CCATAATGGG CGCGGGCTGT CAGGTGCTCA GGGTTTAAAT
    CCAGTCATGA ACATAATCGG AGCAGAGGAT GAAGACTTTG AGAATGATGA GCAGCCTATG
    GTGGACGATC AGTGCATTCA CTTTAACAGT ATTGAGCTGT TGAAGTCCAG GCCCACCCAC
    CTGCTGGTGT TCCTTCATCA TGTCATGCTA CAGTTTGACT GTGCTCCTGT GCTATGCTAC
    CTTCATGCTG ACCTCATGAA GAACCTCAAT ACCAAAGACA CCAAGAAGCA CTTTGGGGAA
    TTCTACAACA CTTTCCTGGA GAAAGGAGCG ATTCTCAAAG TATCTGTACC AAGTAACATC
    GCCCCTGAAA TAGATCGCTT GCGTCCAGAA CTGATCAATG AAGAGCAGCA AAAACGTTAC
    GCTAATGAAA TACAGTTTGC GCAGAGTCCA GAGATACTGC GACAGCTGGA AGACTTCAGG
    CAAAAGAGGA TGATGGGAAT GACACCTAAT GAGCGGGAAT TAAATGATGT GGAATCCCAT
    CGACCCACTG ATCGCATCCC TATGGACATG AAGGAGAAGG CTGTCGCTGA ATCCCTGCTG
    GAAAAGATGG TTGAGGCCAA CCCCTCAATT GTTCCTGACA AGGACAAAAG TAACTCCATC
    TTTGGAGCAG TGGCTTATTA TATGAAGTAT CTTGGAGTCA AGACCAGAGC TTTTGACAAC
    AAGAAGTCCA AGTCAGGATG GCGGCCCTCG GTTCCATCTA TGCTGAAGCC ATGGTCAGTC
    AACAAGCAGA TTAAACCAGT CAAACCGCCT GGAATCGGCT ATGACGGTAA GCAAAGTAGA
    TTGGACTTTG AAGCAAAGCA AAACAAACAA CCTCCTCATC GAGTGTCTGT TAGTGATAGT
    GTCACCCCTC CAGGGCGAAA AACTGGTATT ACCGTGTCAG GGAGCACTCA TGGGTCAGAG
    AGCAGTGAAG AGTTACCCAT CAGTGTGAGT GTGACTCCCA GCCCTGACTT TCAAACTGAT
    ACAGTAGGTA TCATCAACAA TCGCACAGAC CCCAAACCAG CCTCTGAGGG TGGAGAAGTG
    TCTCCAGTCA GCACTGGTGG GACTGTGTCC ATCTGTGAAC CTCTCGCTGT CATTGATGTC
    CCTCCAGAAG ACATTCAGGA CAGCGGCAGG AATGTGCGTC GCAGTGAAAG CCTTTGTGTG
    GAGCGGCGTC ACTCTCGTCG TGGTGGCTCT ACTCGGGCCA AGCAGTCTCG TTCCCGCAGC
    GACGTGGACC TCCAGTCAAC TTCCACATCC CATCCCCTCT CACCCTCCCC ACAGCATTCA
    GTCTCCGTGG AAGGTGACAA AACACCGGAA ATCGCTCTCC TCACTCCCAC TTTTGTCCCG
    GCCCCTCAGC AAGAAGAGAT AGAGCCCCGT CTCCTGGAGC TCGAGCAGGA CCCACTCAAC
    TGGAGAGAAC TTGCTTCCAG TGAAAGCCTG CATAAGCTGA GCAAGAAGGA AGCCAAAAGA
    CAAGAAGTCA TAAATGAGCT GTTTGTGACA GAACAAGCAC ACGTCCGTAT GCTGAGTGTA
    TTGCAGACAG TGTTTTCTCG TCCACTTGAG AAGCAAGAGT ATATGACTGC AAATGAGGTG
    GCCACCATCT TCTCCAGCCT GGATGACATC ATAGAGATGC ACTATGTTTT CTATGAGAAC
    CTGAAGAAAC TGCGTGAAGA AGATGAATAC ATTGTCAAAA CTATCAGCAC ACCACTTCTC
    AACAGATTCA GTGGATCTGA AGGAGAATGG TTTCAAAAGC TCACAGCACG CTTCTGTAGC
    TACCAGTCCT GGGCTCTGGA GCAGATCAAG AGCAGGCAGA AGAAGGACCC CCGATTCAAT
    GCTTTTATAC TGGAAGCAGA GAGTAAGCCA CAGTGTCGGA GGCTACAGCT AAAGGATATC
    ATACCCATAG AGATGCAGAG ACTAACAAAA TACCCTCTTC TGCTGGAAAA CATTGCTAAG
    AACACAGAGG ATGCAACAGA AAAAGAGAAA ATTAACCAAG CAGCAGAATC CTGCCGTAAG
    ATCCTCAACC ATGTCAATGA AGAAGTCAAA CAAATGGAGA ACCTGCTGAT TTTGAAAGAC
    TACCAGCGTC GACTGGATAC CTCAGGACTG AAACCCAGCA ATGAACTCTA CAGTGAGTAC
    AAGACCATTG ATCTGACCAA CCGAACGATG CTGTTTGAAG GACCTCTGAC ATGGAGGGTG
    ACCAAAGAGA AAGCAATCGA TGTGCACTGT GTGTTGCTGT CAGACCTGCT GGTCTTGTTT
    CAGAAGCAGG ATGATAAGAT GCTTTTGAAG TGTCAGAGCA AAAGCAACAT CGCCGTTCAG
    GAAGGCAAGC AGATGCTGAG TCCAATCATC AAGCTGGAGT CTGTTTTCCT CAGAGAGGTG
    GCCACTGACC CGAAGGCCCT GTATGTTATC TTCACCTGGG ACAGTGGAGC ACAGATCTAT
    GAACTGGTGG CTCAGTCTGT CGGGGAGAGG AAAAACTGGA CAGAGGCGAT TAAGTCTGCG
    GTTGATGAGC TGAAGAAGGC ATCAAAGAGC AACACCAAAC TGGAAAGGAA TAGCAGGGCT
    GGCAGTGTGC CAAACAACAT TGCTGTACCT CTATATGGCC CAGCTCATCC TCCTCTTAGC
    CCCACTGAAA ATGGAGGTGA ACTGCTGAAA AATCGCGTCG GGCGATTTAG TGAGAAGATT
    CGAGAAAGTG ATCCTCCACT CATCGACTAT CTAGCAGCCA ACGGCTTTGA CCTGCTCACC
    CACAGCCACA CACCTCCGGA GAAATCTGCT ACTGTTGCTT TGGATGAAGT CATGTGTTTG
    AAGAGGCTAT TGGTTGGGAG TATTAGCCTG TCAGATGATG CTCAGACTCT TGGGGAAAAC
    GGTGGGATGG CACCTGAAAG TCAGGATGCA GATGGAACTC AACTGTCAGT GGAGGAATCG
    AGCACAGAAG CAGGTCCTGA AGTAGAAGAG CATGAGGAAG ATGTAGAGAA TAAAGGAGAA
    GAGGAGAAAG GTATCAGTGC TCCTCTCGTG CTGTCTGAGG AGCGCATGGA GGAGGTGCAG
    ATGAGACTGC AGGCTCTGGA GGAGCAGCTG AAGAGATTGA GGTTGGTTGA GGAGGACCAT
    TATAAATTAC AAGAAGCTCT TGCTAAATAC ACACTTCAGG GGGGCCATTA CAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149244
    Clone ID Related Accession (Same CDS sequence) XM_021466701.1
    Accession Version XM_021466701.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3222bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 1 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCTGTTGT TCTCCATTCT TGTAGGAGCT CGGTTTGCTG GTGTTGGTAG GACCTGCGAC 
    AGCATGGATT ACGAAGACGC CCATAATGGG CGCGGGCTGT CAGGTGCTCA GGGTTTAAAT
    CCAGTCATGA ACATAATCGG AGCAGAGGAT GAAGACTTTG AGAATGATGA GCAGCCTATG
    GTGGACGATC AGTGCATTCA CTTTAACAGT ATTGAGCTGT TGAAGTCCAG GCCCACCCAC
    CTGCTGGTGT TCCTTCATCA TGTCATGCTA CAGTTTGACT GTGCTCCTGT GCTATGCTAC
    CTTCATGCTG ACCTCATGAA GAACCTCAAT ACCAAAGACA CCAAGAAGCA CTTTGGGGAA
    TTCTACAACA CTTTCCTGGA GAAAGGAGCG ATTCTCAAAG TATCTGTACC AAGTAACATC
    GCCCCTGAAA TAGATCGCTT GCGTCCAGAA CTGATCAATG AAGAGCAGCA AAAACGTTAC
    GCTAATGAAA TACAGTTTGC GCAGAGTCCA GAGATACTGC GACAGCTGGA AGACTTCAGG
    CAAAAGAGGA TGATGGGAAT GACACCTAAT GAGCGGGAAT TAAATGATGT GGAATCCCAT
    CGACCCACTG ATCGCATCCC TATGGACATG AAGGAGAAGG CTGTCGCTGA ATCCCTGCTG
    GAAAAGATGG TTGAGGCCAA CCCCTCAATT GTTCCTGACA AGGACAAAAG TAACTCCATC
    TTTGGAGCAG TGGCTTATTA TATGAAGTAT CTTGGAGTCA AGACCAGAGC TTTTGACAAC
    AAGAAGTCCA AGTCAGGATG GCGGCCCTCG GTTCCATCTA TGCTGAAGCC ATGGTCAGTC
    AACAAGCAGA TTAAACCAGT CAAACCGCCT GGAATCGGCT ATGACGCAAA GCAAAACAAA
    CAACCTCCTC ATCGAGTGTC TGTTAGTGAT AGTGTCACCC CTCCAGGGCG AAAAACTGGT
    ATTACCGTGT CAGGGAGCAC TCATGGGTCA GAGAGCAGTG AAGAGTTACC CATCAGTGTG
    AGTGTGACTC CCAGCCCTGA CTTTCAAACT GATACAGTAG GTATCATCAA CAATCGCACA
    GACCCCAAAC CAGCCTCTGA GGGTGGAGAA GTGTCTCCAG TCAGCACTGG TGGGACTGTG
    TCCATCTGTG AACCTCTCGC TGTCATTGAT GTCCCTCCAG AAGACATTCA GGACAGCGGC
    AGAAAGAAGC CAAGGAATGT GCGTCGCAGT GAAAGCCTTT GTGTGGAGCG GCGTCACTCT
    CGTCGTGGTG GCTCTACTCG GGCCAAGCAG TCTCGTTCCC GCAGCGACGT GGACCTCCAG
    TCAACTTCCA CATCCCATCC CCTCTCACCC TCCCCACAGC ATTCAGTCTC CGTGGAAGGT
    GACAAAACAC CGGAAATCGC TCTCCTCACT CCCACTTTTG TCCCGGCCCC TCAGCAAGAA
    GAGATAGAGC CCCGTCTCCT GGAGCTCGAG CAGGACCCAC TCAACTGGAG AGAACTTGCT
    TCCAGTGAAA GCCTGCATAA GCTGAGCAAG AAGGAAGCCA AAAGACAAGA AGTCATAAAT
    GAGCTGTTTG TGACAGAACA AGCACACGTC CGTATGCTGA GTGTATTGCA GACAGTGTTT
    TCTCGTCCAC TTGAGAAGCA AGAGTATATG ACTGCAAATG AGGTGGCCAC CATCTTCTCC
    AGCCTGGATG ACATCATAGA GATGCACTAT GTTTTCTATG AGAACCTGAA GAAACTGCGT
    GAAGAAGATG AATACATTGT CAAAACTATC AGCACACCAC TTCTCAACAG ATTCAGTGGA
    TCTGAAGGAG AATGGTTTCA AAAGCTCACA GCACGCTTCT GTAGCTACCA GTCCTGGGCT
    CTGGAGCAGA TCAAGAGCAG GCAGAAGAAG GACCCCCGAT TCAATGCTTT TATACTGGAA
    GCAGAGAGTA AGCCACAGTG TCGGAGGCTA CAGCTAAAGG ATATCATACC CATAGAGATG
    CAGAGACTAA CAAAATACCC TCTTCTGCTG GAAAACATTG CTAAGAACAC AGAGGATGCA
    ACAGAAAAAG AGAAAATTAA CCAAGCAGCA GAATCCTGCC GTAAGATCCT CAACCATGTC
    AATGAAGAAG TCAAACAAAT GGAGAACCTG CTGATTTTGA AAGACTACCA GCGTCGACTG
    GATACCTCAG GACTGAAACC CAGCAATGAA CTCTACAGTG AGTACAAGAC CATTGATCTG
    ACCAACCGAA CGATGCTGTT TGAAGGACCT CTGACATGGA GGGTGACCAA AGAGAAAGCA
    ATCGATGTGC ACTGTGTGTT GCTGTCAGAC CTGCTGGTCT TGTTTCAGAA GCAGGATGAT
    AAGATGCTTT TGAAGTGTCA GAGCAAAAGC AACATCGCCG TTCAGGAAGG CAAGCAGATG
    CTGAGTCCAA TCATCAAGCT GGAGTCTGTT TTCCTCAGAG AGGTGGCCAC TGACCCGAAG
    GCCCTGTATG TTATCTTCAC CTGGGACAGT GGAGCACAGA TCTATGAACT GGTGGCTCAG
    TCTGTCGGGG AGAGGAAAAA CTGGACAGAG GCGATTAAGT CTGCGGTTGA TGAGCTGAAG
    AAGGCATCAA AGAGCAACAC CAAACTGGAA AGGAATAGCA GGGCTGGCAG TGTGCCAAAC
    AACATTGCTG TACCTCTATA TGGCCCAGCT CATCCTCCTC TTAGCCCCAC TGAAAATGGA
    GGTGAACTGC TGAAAAATCG CGTCGGGCGA TTTAGTGAGA AGATTCGAGA AAGTGATCCT
    CCACTCATCG ACTATCTAGC AGCCAACGGC TTTGACCTGC TCACCCACAG CCACACACCT
    CCGGAGAAAT CTGCTACTGT TGCTTTGGAT GAAGTCATGT GTTTGAAGAG GCTATTGGTT
    GGGAGTATTA GCCTGTCAGA TGATGCTCAG ACTCTTGGGG AAAACGGTGG GATGGCACCT
    GAAAGTCAGG ATGCAGATGG AACTCAACTG TCAGTGGAGG AATCGAGCAC AGAAGCAGGT
    CCTGAAGTAG AAGAGCATGA GGAAGATGTA GAGAATAAAG GAGAAGAGGA GAAAGGTATC
    AGTGCTCCTC TCGTGCTGTC TGAGGAGCGC ATGGAGGAGG TGCAGATGAG ACTGCAGGCT
    CTGGAGGAGC AGCTGAAGAG ATTGAGGTTG GTTGAGGAGG ACCATTATAA ATTACAAGAA
    GCTCTTGCTA AATACACACT TCAGGGGGGC CATTACAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322376.1
    CDS262..3483
    Translation

    Target ORF information:

    RefSeq Version XM_021466701.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 1b (arhgef1b), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466701.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCTGTTGT TCTCCATTCT TGTAGGAGCT CGGTTTGCTG GTGTTGGTAG GACCTGCGAC 
    AGCATGGATT ACGAAGACGC CCATAATGGG CGCGGGCTGT CAGGTGCTCA GGGTTTAAAT
    CCAGTCATGA ACATAATCGG AGCAGAGGAT GAAGACTTTG AGAATGATGA GCAGCCTATG
    GTGGACGATC AGTGCATTCA CTTTAACAGT ATTGAGCTGT TGAAGTCCAG GCCCACCCAC
    CTGCTGGTGT TCCTTCATCA TGTCATGCTA CAGTTTGACT GTGCTCCTGT GCTATGCTAC
    CTTCATGCTG ACCTCATGAA GAACCTCAAT ACCAAAGACA CCAAGAAGCA CTTTGGGGAA
    TTCTACAACA CTTTCCTGGA GAAAGGAGCG ATTCTCAAAG TATCTGTACC AAGTAACATC
    GCCCCTGAAA TAGATCGCTT GCGTCCAGAA CTGATCAATG AAGAGCAGCA AAAACGTTAC
    GCTAATGAAA TACAGTTTGC GCAGAGTCCA GAGATACTGC GACAGCTGGA AGACTTCAGG
    CAAAAGAGGA TGATGGGAAT GACACCTAAT GAGCGGGAAT TAAATGATGT GGAATCCCAT
    CGACCCACTG ATCGCATCCC TATGGACATG AAGGAGAAGG CTGTCGCTGA ATCCCTGCTG
    GAAAAGATGG TTGAGGCCAA CCCCTCAATT GTTCCTGACA AGGACAAAAG TAACTCCATC
    TTTGGAGCAG TGGCTTATTA TATGAAGTAT CTTGGAGTCA AGACCAGAGC TTTTGACAAC
    AAGAAGTCCA AGTCAGGATG GCGGCCCTCG GTTCCATCTA TGCTGAAGCC ATGGTCAGTC
    AACAAGCAGA TTAAACCAGT CAAACCGCCT GGAATCGGCT ATGACGCAAA GCAAAACAAA
    CAACCTCCTC ATCGAGTGTC TGTTAGTGAT AGTGTCACCC CTCCAGGGCG AAAAACTGGT
    ATTACCGTGT CAGGGAGCAC TCATGGGTCA GAGAGCAGTG AAGAGTTACC CATCAGTGTG
    AGTGTGACTC CCAGCCCTGA CTTTCAAACT GATACAGTAG GTATCATCAA CAATCGCACA
    GACCCCAAAC CAGCCTCTGA GGGTGGAGAA GTGTCTCCAG TCAGCACTGG TGGGACTGTG
    TCCATCTGTG AACCTCTCGC TGTCATTGAT GTCCCTCCAG AAGACATTCA GGACAGCGGC
    AGAAAGAAGC CAAGGAATGT GCGTCGCAGT GAAAGCCTTT GTGTGGAGCG GCGTCACTCT
    CGTCGTGGTG GCTCTACTCG GGCCAAGCAG TCTCGTTCCC GCAGCGACGT GGACCTCCAG
    TCAACTTCCA CATCCCATCC CCTCTCACCC TCCCCACAGC ATTCAGTCTC CGTGGAAGGT
    GACAAAACAC CGGAAATCGC TCTCCTCACT CCCACTTTTG TCCCGGCCCC TCAGCAAGAA
    GAGATAGAGC CCCGTCTCCT GGAGCTCGAG CAGGACCCAC TCAACTGGAG AGAACTTGCT
    TCCAGTGAAA GCCTGCATAA GCTGAGCAAG AAGGAAGCCA AAAGACAAGA AGTCATAAAT
    GAGCTGTTTG TGACAGAACA AGCACACGTC CGTATGCTGA GTGTATTGCA GACAGTGTTT
    TCTCGTCCAC TTGAGAAGCA AGAGTATATG ACTGCAAATG AGGTGGCCAC CATCTTCTCC
    AGCCTGGATG ACATCATAGA GATGCACTAT GTTTTCTATG AGAACCTGAA GAAACTGCGT
    GAAGAAGATG AATACATTGT CAAAACTATC AGCACACCAC TTCTCAACAG ATTCAGTGGA
    TCTGAAGGAG AATGGTTTCA AAAGCTCACA GCACGCTTCT GTAGCTACCA GTCCTGGGCT
    CTGGAGCAGA TCAAGAGCAG GCAGAAGAAG GACCCCCGAT TCAATGCTTT TATACTGGAA
    GCAGAGAGTA AGCCACAGTG TCGGAGGCTA CAGCTAAAGG ATATCATACC CATAGAGATG
    CAGAGACTAA CAAAATACCC TCTTCTGCTG GAAAACATTG CTAAGAACAC AGAGGATGCA
    ACAGAAAAAG AGAAAATTAA CCAAGCAGCA GAATCCTGCC GTAAGATCCT CAACCATGTC
    AATGAAGAAG TCAAACAAAT GGAGAACCTG CTGATTTTGA AAGACTACCA GCGTCGACTG
    GATACCTCAG GACTGAAACC CAGCAATGAA CTCTACAGTG AGTACAAGAC CATTGATCTG
    ACCAACCGAA CGATGCTGTT TGAAGGACCT CTGACATGGA GGGTGACCAA AGAGAAAGCA
    ATCGATGTGC ACTGTGTGTT GCTGTCAGAC CTGCTGGTCT TGTTTCAGAA GCAGGATGAT
    AAGATGCTTT TGAAGTGTCA GAGCAAAAGC AACATCGCCG TTCAGGAAGG CAAGCAGATG
    CTGAGTCCAA TCATCAAGCT GGAGTCTGTT TTCCTCAGAG AGGTGGCCAC TGACCCGAAG
    GCCCTGTATG TTATCTTCAC CTGGGACAGT GGAGCACAGA TCTATGAACT GGTGGCTCAG
    TCTGTCGGGG AGAGGAAAAA CTGGACAGAG GCGATTAAGT CTGCGGTTGA TGAGCTGAAG
    AAGGCATCAA AGAGCAACAC CAAACTGGAA AGGAATAGCA GGGCTGGCAG TGTGCCAAAC
    AACATTGCTG TACCTCTATA TGGCCCAGCT CATCCTCCTC TTAGCCCCAC TGAAAATGGA
    GGTGAACTGC TGAAAAATCG CGTCGGGCGA TTTAGTGAGA AGATTCGAGA AAGTGATCCT
    CCACTCATCG ACTATCTAGC AGCCAACGGC TTTGACCTGC TCACCCACAG CCACACACCT
    CCGGAGAAAT CTGCTACTGT TGCTTTGGAT GAAGTCATGT GTTTGAAGAG GCTATTGGTT
    GGGAGTATTA GCCTGTCAGA TGATGCTCAG ACTCTTGGGG AAAACGGTGG GATGGCACCT
    GAAAGTCAGG ATGCAGATGG AACTCAACTG TCAGTGGAGG AATCGAGCAC AGAAGCAGGT
    CCTGAAGTAG AAGAGCATGA GGAAGATGTA GAGAATAAAG GAGAAGAGGA GAAAGGTATC
    AGTGCTCCTC TCGTGCTGTC TGAGGAGCGC ATGGAGGAGG TGCAGATGAG ACTGCAGGCT
    CTGGAGGAGC AGCTGAAGAG ATTGAGGTTG GTTGAGGAGG ACCATTATAA ATTACAAGAA
    GCTCTTGCTA AATACACACT TCAGGGGGGC CATTACAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149245
    Clone ID Related Accession (Same CDS sequence) XM_021466702.1
    Accession Version XM_021466702.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3216bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 1 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCTGTTGT TCTCCATTCT TGTAGGAGCT CGGTTTGCTG GTGTTGGTAG GACCTGCGAC 
    AGCATGGATT ACGAAGACGC CCATAATGGG CGCGGGCTGT CAGGTGCTCA GGGTTTAAAT
    CCAGTCATGA ACATAATCGG AGCAGAGGAT GAAGACTTTG AGAATGATGA GCAGCCTATG
    GTGGACGATC AGTGCATTCA CTTTAACAGT ATTGAGCTGT TGAAGTCCAG GCCCACCCAC
    CTGCTGGTGT TCCTTCATCA TGTCATGCTA CAGTTTGACT GTGCTCCTGT GCTATGCTAC
    CTTCATGCTG ACCTCATGAA GAACCTCAAT ACCAAAGACA CCAAGAAGCA CTTTGGGGAA
    TTCTACAACA CTTTCCTGGA GAAAGGAGCG ATTCTCAAAG TATCTGTACC AAGTAACATC
    GCCCCTGAAA TAGATCGCTT GCGTCCAGAA CTGATCAATG AAGAGCAGCA AAAACGTTAC
    GCTAATGAAA TACAGTTTGC GCAGAGTCCA GAGATACTGC GACAGCTGGA AGACTTCAGG
    CAAAAGAGGA TGATGGGAAT GACACCTAAT GAGCGGGAAT TAAATGATGT GGAATCCCAT
    CGACCCACTG ATCGCATCCC TATGGACATG AAGGAGAAGG CTGTCGCTGA ATCCCTGCTG
    GAAAAGATGG TTGAGGCCAA CCCCTCAATT GTTCCTGACA AGGACAAAAG TAACTCCATC
    TTTGGAGCAG TGGCTTATTA TATGAAGTAT CTTGGAGTCA AGACCAGAGC TTTTGACAAC
    AAGAAGTCCA AGTCAGGATG GCGGCCCTCG GTTCCATCTA TGCTGAAGCC ATGGTCAGTC
    AACAAGCAGA TTAAACCAGT CAAACCGCCT GGAATCGGCT CAAAGCAAAA CAAACAACCT
    CCTCATCGAG TGTCTGTTAG TGATAGTGTC ACCCCTCCAG GGCGAAAAAC TGGTATTACC
    GTGTCAGGGA GCACTCATGG GTCAGAGAGC AGTGAAGAGT TACCCATCAG TGTGAGTGTG
    ACTCCCAGCC CTGACTTTCA AACTGATACA GTAGGTATCA TCAACAATCG CACAGACCCC
    AAACCAGCCT CTGAGGGTGG AGAAGTGTCT CCAGTCAGCA CTGGTGGGAC TGTGTCCATC
    TGTGAACCTC TCGCTGTCAT TGATGTCCCT CCAGAAGACA TTCAGGACAG CGGCAGAAAG
    AAGCCAAGGA ATGTGCGTCG CAGTGAAAGC CTTTGTGTGG AGCGGCGTCA CTCTCGTCGT
    GGTGGCTCTA CTCGGGCCAA GCAGTCTCGT TCCCGCAGCG ACGTGGACCT CCAGTCAACT
    TCCACATCCC ATCCCCTCTC ACCCTCCCCA CAGCATTCAG TCTCCGTGGA AGGTGACAAA
    ACACCGGAAA TCGCTCTCCT CACTCCCACT TTTGTCCCGG CCCCTCAGCA AGAAGAGATA
    GAGCCCCGTC TCCTGGAGCT CGAGCAGGAC CCACTCAACT GGAGAGAACT TGCTTCCAGT
    GAAAGCCTGC ATAAGCTGAG CAAGAAGGAA GCCAAAAGAC AAGAAGTCAT AAATGAGCTG
    TTTGTGACAG AACAAGCACA CGTCCGTATG CTGAGTGTAT TGCAGACAGT GTTTTCTCGT
    CCACTTGAGA AGCAAGAGTA TATGACTGCA AATGAGGTGG CCACCATCTT CTCCAGCCTG
    GATGACATCA TAGAGATGCA CTATGTTTTC TATGAGAACC TGAAGAAACT GCGTGAAGAA
    GATGAATACA TTGTCAAAAC TATCAGCACA CCACTTCTCA ACAGATTCAG TGGATCTGAA
    GGAGAATGGT TTCAAAAGCT CACAGCACGC TTCTGTAGCT ACCAGTCCTG GGCTCTGGAG
    CAGATCAAGA GCAGGCAGAA GAAGGACCCC CGATTCAATG CTTTTATACT GGAAGCAGAG
    AGTAAGCCAC AGTGTCGGAG GCTACAGCTA AAGGATATCA TACCCATAGA GATGCAGAGA
    CTAACAAAAT ACCCTCTTCT GCTGGAAAAC ATTGCTAAGA ACACAGAGGA TGCAACAGAA
    AAAGAGAAAA TTAACCAAGC AGCAGAATCC TGCCGTAAGA TCCTCAACCA TGTCAATGAA
    GAAGTCAAAC AAATGGAGAA CCTGCTGATT TTGAAAGACT ACCAGCGTCG ACTGGATACC
    TCAGGACTGA AACCCAGCAA TGAACTCTAC AGTGAGTACA AGACCATTGA TCTGACCAAC
    CGAACGATGC TGTTTGAAGG ACCTCTGACA TGGAGGGTGA CCAAAGAGAA AGCAATCGAT
    GTGCACTGTG TGTTGCTGTC AGACCTGCTG GTCTTGTTTC AGAAGCAGGA TGATAAGATG
    CTTTTGAAGT GTCAGAGCAA AAGCAACATC GCCGTTCAGG AAGGCAAGCA GATGCTGAGT
    CCAATCATCA AGCTGGAGTC TGTTTTCCTC AGAGAGGTGG CCACTGACCC GAAGGCCCTG
    TATGTTATCT TCACCTGGGA CAGTGGAGCA CAGATCTATG AACTGGTGGC TCAGTCTGTC
    GGGGAGAGGA AAAACTGGAC AGAGGCGATT AAGTCTGCGG TTGATGAGCT GAAGAAGGCA
    TCAAAGAGCA ACACCAAACT GGAAAGGAAT AGCAGGGCTG GCAGTGTGCC AAACAACATT
    GCTGTACCTC TATATGGCCC AGCTCATCCT CCTCTTAGCC CCACTGAAAA TGGAGGTGAA
    CTGCTGAAAA ATCGCGTCGG GCGATTTAGT GAGAAGATTC GAGAAAGTGA TCCTCCACTC
    ATCGACTATC TAGCAGCCAA CGGCTTTGAC CTGCTCACCC ACAGCCACAC ACCTCCGGAG
    AAATCTGCTA CTGTTGCTTT GGATGAAGTC ATGTGTTTGA AGAGGCTATT GGTTGGGAGT
    ATTAGCCTGT CAGATGATGC TCAGACTCTT GGGGAAAACG GTGGGATGGC ACCTGAAAGT
    CAGGATGCAG ATGGAACTCA ACTGTCAGTG GAGGAATCGA GCACAGAAGC AGGTCCTGAA
    GTAGAAGAGC ATGAGGAAGA TGTAGAGAAT AAAGGAGAAG AGGAGAAAGG TATCAGTGCT
    CCTCTCGTGC TGTCTGAGGA GCGCATGGAG GAGGTGCAGA TGAGACTGCA GGCTCTGGAG
    GAGCAGCTGA AGAGATTGAG GTTGGTTGAG GAGGACCATT ATAAATTACA AGAAGCTCTT
    GCTAAATACA CACTTCAGGG GGGCCATTAC AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322377.1
    CDS262..3477
    Translation

    Target ORF information:

    RefSeq Version XM_021466702.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 1b (arhgef1b), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466702.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGCTGTTGT TCTCCATTCT TGTAGGAGCT CGGTTTGCTG GTGTTGGTAG GACCTGCGAC 
    AGCATGGATT ACGAAGACGC CCATAATGGG CGCGGGCTGT CAGGTGCTCA GGGTTTAAAT
    CCAGTCATGA ACATAATCGG AGCAGAGGAT GAAGACTTTG AGAATGATGA GCAGCCTATG
    GTGGACGATC AGTGCATTCA CTTTAACAGT ATTGAGCTGT TGAAGTCCAG GCCCACCCAC
    CTGCTGGTGT TCCTTCATCA TGTCATGCTA CAGTTTGACT GTGCTCCTGT GCTATGCTAC
    CTTCATGCTG ACCTCATGAA GAACCTCAAT ACCAAAGACA CCAAGAAGCA CTTTGGGGAA
    TTCTACAACA CTTTCCTGGA GAAAGGAGCG ATTCTCAAAG TATCTGTACC AAGTAACATC
    GCCCCTGAAA TAGATCGCTT GCGTCCAGAA CTGATCAATG AAGAGCAGCA AAAACGTTAC
    GCTAATGAAA TACAGTTTGC GCAGAGTCCA GAGATACTGC GACAGCTGGA AGACTTCAGG
    CAAAAGAGGA TGATGGGAAT GACACCTAAT GAGCGGGAAT TAAATGATGT GGAATCCCAT
    CGACCCACTG ATCGCATCCC TATGGACATG AAGGAGAAGG CTGTCGCTGA ATCCCTGCTG
    GAAAAGATGG TTGAGGCCAA CCCCTCAATT GTTCCTGACA AGGACAAAAG TAACTCCATC
    TTTGGAGCAG TGGCTTATTA TATGAAGTAT CTTGGAGTCA AGACCAGAGC TTTTGACAAC
    AAGAAGTCCA AGTCAGGATG GCGGCCCTCG GTTCCATCTA TGCTGAAGCC ATGGTCAGTC
    AACAAGCAGA TTAAACCAGT CAAACCGCCT GGAATCGGCT CAAAGCAAAA CAAACAACCT
    CCTCATCGAG TGTCTGTTAG TGATAGTGTC ACCCCTCCAG GGCGAAAAAC TGGTATTACC
    GTGTCAGGGA GCACTCATGG GTCAGAGAGC AGTGAAGAGT TACCCATCAG TGTGAGTGTG
    ACTCCCAGCC CTGACTTTCA AACTGATACA GTAGGTATCA TCAACAATCG CACAGACCCC
    AAACCAGCCT CTGAGGGTGG AGAAGTGTCT CCAGTCAGCA CTGGTGGGAC TGTGTCCATC
    TGTGAACCTC TCGCTGTCAT TGATGTCCCT CCAGAAGACA TTCAGGACAG CGGCAGAAAG
    AAGCCAAGGA ATGTGCGTCG CAGTGAAAGC CTTTGTGTGG AGCGGCGTCA CTCTCGTCGT
    GGTGGCTCTA CTCGGGCCAA GCAGTCTCGT TCCCGCAGCG ACGTGGACCT CCAGTCAACT
    TCCACATCCC ATCCCCTCTC ACCCTCCCCA CAGCATTCAG TCTCCGTGGA AGGTGACAAA
    ACACCGGAAA TCGCTCTCCT CACTCCCACT TTTGTCCCGG CCCCTCAGCA AGAAGAGATA
    GAGCCCCGTC TCCTGGAGCT CGAGCAGGAC CCACTCAACT GGAGAGAACT TGCTTCCAGT
    GAAAGCCTGC ATAAGCTGAG CAAGAAGGAA GCCAAAAGAC AAGAAGTCAT AAATGAGCTG
    TTTGTGACAG AACAAGCACA CGTCCGTATG CTGAGTGTAT TGCAGACAGT GTTTTCTCGT
    CCACTTGAGA AGCAAGAGTA TATGACTGCA AATGAGGTGG CCACCATCTT CTCCAGCCTG
    GATGACATCA TAGAGATGCA CTATGTTTTC TATGAGAACC TGAAGAAACT GCGTGAAGAA
    GATGAATACA TTGTCAAAAC TATCAGCACA CCACTTCTCA ACAGATTCAG TGGATCTGAA
    GGAGAATGGT TTCAAAAGCT CACAGCACGC TTCTGTAGCT ACCAGTCCTG GGCTCTGGAG
    CAGATCAAGA GCAGGCAGAA GAAGGACCCC CGATTCAATG CTTTTATACT GGAAGCAGAG
    AGTAAGCCAC AGTGTCGGAG GCTACAGCTA AAGGATATCA TACCCATAGA GATGCAGAGA
    CTAACAAAAT ACCCTCTTCT GCTGGAAAAC ATTGCTAAGA ACACAGAGGA TGCAACAGAA
    AAAGAGAAAA TTAACCAAGC AGCAGAATCC TGCCGTAAGA TCCTCAACCA TGTCAATGAA
    GAAGTCAAAC AAATGGAGAA CCTGCTGATT TTGAAAGACT ACCAGCGTCG ACTGGATACC
    TCAGGACTGA AACCCAGCAA TGAACTCTAC AGTGAGTACA AGACCATTGA TCTGACCAAC
    CGAACGATGC TGTTTGAAGG ACCTCTGACA TGGAGGGTGA CCAAAGAGAA AGCAATCGAT
    GTGCACTGTG TGTTGCTGTC AGACCTGCTG GTCTTGTTTC AGAAGCAGGA TGATAAGATG
    CTTTTGAAGT GTCAGAGCAA AAGCAACATC GCCGTTCAGG AAGGCAAGCA GATGCTGAGT
    CCAATCATCA AGCTGGAGTC TGTTTTCCTC AGAGAGGTGG CCACTGACCC GAAGGCCCTG
    TATGTTATCT TCACCTGGGA CAGTGGAGCA CAGATCTATG AACTGGTGGC TCAGTCTGTC
    GGGGAGAGGA AAAACTGGAC AGAGGCGATT AAGTCTGCGG TTGATGAGCT GAAGAAGGCA
    TCAAAGAGCA ACACCAAACT GGAAAGGAAT AGCAGGGCTG GCAGTGTGCC AAACAACATT
    GCTGTACCTC TATATGGCCC AGCTCATCCT CCTCTTAGCC CCACTGAAAA TGGAGGTGAA
    CTGCTGAAAA ATCGCGTCGG GCGATTTAGT GAGAAGATTC GAGAAAGTGA TCCTCCACTC
    ATCGACTATC TAGCAGCCAA CGGCTTTGAC CTGCTCACCC ACAGCCACAC ACCTCCGGAG
    AAATCTGCTA CTGTTGCTTT GGATGAAGTC ATGTGTTTGA AGAGGCTATT GGTTGGGAGT
    ATTAGCCTGT CAGATGATGC TCAGACTCTT GGGGAAAACG GTGGGATGGC ACCTGAAAGT
    CAGGATGCAG ATGGAACTCA ACTGTCAGTG GAGGAATCGA GCACAGAAGC AGGTCCTGAA
    GTAGAAGAGC ATGAGGAAGA TGTAGAGAAT AAAGGAGAAG AGGAGAAAGG TATCAGTGCT
    CCTCTCGTGC TGTCTGAGGA GCGCATGGAG GAGGTGCAGA TGAGACTGCA GGCTCTGGAG
    GAGCAGCTGA AGAGATTGAG GTTGGTTGAG GAGGACCATT ATAAATTACA AGAAGCTCTT
    GCTAAATACA CACTTCAGGG GGGCCATTAC AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149246
    Clone ID Related Accession (Same CDS sequence) XM_017351352.2
    Accession Version XM_017351352.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3186bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 1 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017351352.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATTACG AAGACGCCCA TAATGGGCGC GGGCTGTCAG GTGCTCAGGG TTTAAATCCA 
    GTCATGAACA TAATCGGAGC AGAGGATGAA GACTTTGAGA ATGATGAGCA GCCTATGGTG
    GACGATCAGT GCATTCACTT TAACAGTATT GAGCTGTTGA AGTCCAGGCC CACCCACCTG
    CTGGTGTTCC TTCATCATGT CATGCTACAG TTTGACTGTG CTCCTGTGCT ATGCTACCTT
    CATGCTGACC TCATGAAGAA CCTCAATACC AAAGACACCA AGAAGCACTT TGGGGAATTC
    TACAACACTT TCCTGGAGAA AGGAGCGATT CTCAAAGTAT CTGTACCAAG TAACATCGCC
    CCTGAAATAG ATCGCTTGCG TCCAGAACTG ATCAATGAAG AGCAGCAAAA ACGTTACGCT
    AATGAAATAC AGTTTGCGCA GAGTCCAGAG ATACTGCGAC AGCTGGAAGA CTTCAGGCAA
    AAGAGGATGA TGGGAATGAC ACCTAATGAG CGGGAATTAA ATGATGTGGA ATCCCATCGA
    CCCACTGATC GCATCCCTAT GGACATGAAG GAGAAGGCTG TCGCTGAATC CCTGCTGGAA
    AAGATGGTTG AGGCCAACCC CTCAATTGTT CCTGACAAGG ACAAAAGTAA CTCCATCTTT
    GGAGCAGTGG CTTATTATAT GAAGTATCTT GGAGTCAAGA CCAGAGCTTT TGACAACAAG
    AAGTCCAAGT CAGGATGGCG GCCCTCGGTT CCATCTATGC TGAAGCCATG GTCAGTCAAC
    AAGCAGATTA AACCAGTCAA ACCGCCTGGA ATCGGCTATG ACGGTAAGCA AAGTAGATTG
    GACTTTGAAG CAAAGCAAAA CAAACAACCT CCTCATCGAG TGTCTGTTAG TGATAGTGTC
    ACCCCTCCAG GGCGAAAAAC TGGTATTACC GTGTCAGGGA GCACTCATGG GTCAGAGAGC
    AGTGAAGAGT TACCCATCAG TGTGAGTGTG ACTCCCAGCC CTGACTTTCA AACTGATACA
    GTAGGTATCA TCAACAATCG CACAGACCCC AAACCAGCCT CTGAGGGTGG AGAAGTGTCT
    CCAGTCAGCA CTGGTGGGAC TGTGTCCATC TGTGAACCTC TCGCTGTCAT TGATGTCCCT
    CCAGAAGACA TTCAGGACAG CGGCAGAAAG AAGCCAAGGA ATGTGCGTCG CAGTGAAAGC
    CTTTGTGTGG AGCGGCGTCA CTCTCGTCGT GGTGGCTCTA CTCGGGCCAA GCAGTCTCGT
    TCCCGCAGCG ACGTGGACCT CCAGTCAACT TCCACATCCC ATCCCCTCTC ACCCTCCCCA
    CAGCATTCAG TCTCCGTGGA AGGTGACAAA ACACCGGAAA TCGCTCTCCT CACTCCCACT
    TTTGTCCCGG CCCCTCAGCA AGAAGAGATA GAGCCCCGTC TCCTGGAGCT CGAGCAGGAC
    CCACTCAACT GGAGAGAACT TGCTTCCAGT GAAAGCCTGC ATAAGCTGAG CAAGAAGGAA
    GCCAAAAGAC AAGAAGTCAT AAATGAGCTG TTTGTGACAG AACAAGCACA CGTCCGTATG
    CTGAGTGTAT TGCAGACAGT GTTTTCTCGT CCACTTGAGA AGCAAGAGTA TATGACTGCA
    AATGAGGTGG CCACCATCTT CTCCAGCCTG GATGACATCA TAGAGATGCA CTATGTTTTC
    TATGAGAACC TGAAGAAACT GCGTGAAGAA GATGAATACA TTGTCAAAAC TATCAGCACA
    CCACTTCTCA ACAGATTCAG TGGATCTGAA GGAGAATGGT TTCAAAAGCT CACAGCACGC
    TTCTGTAGCT ACCAGTCCTG GGCTCTGGAG CAGATCAAGA GCAGGCAGAA GAAGGACCCC
    CGATTCAATG CTTTTATACT GGAAGCAGAG AGTAAGCCAC AGTGTCGGAG GCTACAGCTA
    AAGGATATCA TACCCATAGA GATGCAGAGA CTAACAAAAT ACCCTCTTCT GCTGGAAAAC
    ATTGCTAAGA ACACAGAGGA TGCAACAGAA AAAGAGAAAA TTAACCAAGC AGCAGAATCC
    TGCCGTAAGA TCCTCAACCA TGTCAATGAA GAAGTCAAAC AAATGGAGAA CCTGCTGATT
    TTGAAAGACT ACCAGCGTCG ACTGGATACC TCAGGACTGA AACCCAGCAA TGAACTCTAC
    AGTGAGTACA AGACCATTGA TCTGACCAAC CGAACGATGC TGTTTGAAGG ACCTCTGACA
    TGGAGGGTGA CCAAAGAGAA AGCAATCGAT GTGCACTGTG TGTTGCTGTC AGACCTGCTG
    GTCTTGTTTC AGAAGCAGGA TGATAAGATG CTTTTGAAGT GTCAGAGCAA AAGCAACATC
    GCCGTTCAGG AAGGCAAGCA GATGCTGAGT CCAATCATCA AGCTGGAGTC TGTTTTCCTC
    AGAGAGGTGG CCACTGACCC GAAGGCCCTG TATGTTATCT TCACCTGGGA CAGTGGAGCA
    CAGATCTATG AACTGGTGGC TCAGTCTGTC GGGGAGAGGA AAAACTGGAC AGAGGCGATT
    AAGTCTGCGG TTGATGAGCT GAAGAAGGCA TCAAAGAGCA ACACCAAACT GGAAAGGAAT
    AGCAGGGCTG GCAGTGTGCC AAACAACATT GCTGTACCTC TATATGGCCC AGCTCATCCT
    CCTCTTAGCC CCACTGAAAA TGGAGGTGAA CTGCTGAAAA ATCGCGTCGG GCGATTTAGT
    GAGAAGATTC GAGAAAGTGA TCCTCCACTC ATCGACTATC TAGCAGCCAA CGGCTTTGAC
    CTGCTCACCC ACAGCCACAC ACCTCCGGAG AAATCTGCTA CTGTTGCTTT GGATGAAGTC
    ATGTGTTTGA AGAGGCTATT GGTTGGGAGT ATTAGCCTGT CAGATGATGC TCAGACTCTT
    GGGGAAAACG GTGGGATGGC ACCTGAAAGT CAGGATGCAG ATGGAACTCA ACTGTCAGTG
    GAGGAATCGA GCACAGAAGC AGGTCCTGAA GTAGAAGAGC ATGAGGAAGA TGTAGAGAAT
    AAAGGAGAAG AGGAGAAAGG TATCAGTGCT CCTCTCGTGC TGTCTGAGGA GCGCATGGAG
    GAGGTGCAGA TGAGACTGCA GGCTCTGGAG GAGCAGCTGA AGAGATTGAG GTTGGTTGAG
    GAGGACCATT ATAAATTACA AGAAGCTCTT GCTAAATACA CACTTCAGGG GGGCCATTAC
    AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017206841.2
    CDS340..3525
    Translation

    Target ORF information:

    RefSeq Version XM_017351352.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 1b (arhgef1b), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351352.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    ATGGATTACG AAGACGCCCA TAATGGGCGC GGGCTGTCAG GTGCTCAGGG TTTAAATCCA 
    GTCATGAACA TAATCGGAGC AGAGGATGAA GACTTTGAGA ATGATGAGCA GCCTATGGTG
    GACGATCAGT GCATTCACTT TAACAGTATT GAGCTGTTGA AGTCCAGGCC CACCCACCTG
    CTGGTGTTCC TTCATCATGT CATGCTACAG TTTGACTGTG CTCCTGTGCT ATGCTACCTT
    CATGCTGACC TCATGAAGAA CCTCAATACC AAAGACACCA AGAAGCACTT TGGGGAATTC
    TACAACACTT TCCTGGAGAA AGGAGCGATT CTCAAAGTAT CTGTACCAAG TAACATCGCC
    CCTGAAATAG ATCGCTTGCG TCCAGAACTG ATCAATGAAG AGCAGCAAAA ACGTTACGCT
    AATGAAATAC AGTTTGCGCA GAGTCCAGAG ATACTGCGAC AGCTGGAAGA CTTCAGGCAA
    AAGAGGATGA TGGGAATGAC ACCTAATGAG CGGGAATTAA ATGATGTGGA ATCCCATCGA
    CCCACTGATC GCATCCCTAT GGACATGAAG GAGAAGGCTG TCGCTGAATC CCTGCTGGAA
    AAGATGGTTG AGGCCAACCC CTCAATTGTT CCTGACAAGG ACAAAAGTAA CTCCATCTTT
    GGAGCAGTGG CTTATTATAT GAAGTATCTT GGAGTCAAGA CCAGAGCTTT TGACAACAAG
    AAGTCCAAGT CAGGATGGCG GCCCTCGGTT CCATCTATGC TGAAGCCATG GTCAGTCAAC
    AAGCAGATTA AACCAGTCAA ACCGCCTGGA ATCGGCTATG ACGGTAAGCA AAGTAGATTG
    GACTTTGAAG CAAAGCAAAA CAAACAACCT CCTCATCGAG TGTCTGTTAG TGATAGTGTC
    ACCCCTCCAG GGCGAAAAAC TGGTATTACC GTGTCAGGGA GCACTCATGG GTCAGAGAGC
    AGTGAAGAGT TACCCATCAG TGTGAGTGTG ACTCCCAGCC CTGACTTTCA AACTGATACA
    GTAGGTATCA TCAACAATCG CACAGACCCC AAACCAGCCT CTGAGGGTGG AGAAGTGTCT
    CCAGTCAGCA CTGGTGGGAC TGTGTCCATC TGTGAACCTC TCGCTGTCAT TGATGTCCCT
    CCAGAAGACA TTCAGGACAG CGGCAGAAAG AAGCCAAGGA ATGTGCGTCG CAGTGAAAGC
    CTTTGTGTGG AGCGGCGTCA CTCTCGTCGT GGTGGCTCTA CTCGGGCCAA GCAGTCTCGT
    TCCCGCAGCG ACGTGGACCT CCAGTCAACT TCCACATCCC ATCCCCTCTC ACCCTCCCCA
    CAGCATTCAG TCTCCGTGGA AGGTGACAAA ACACCGGAAA TCGCTCTCCT CACTCCCACT
    TTTGTCCCGG CCCCTCAGCA AGAAGAGATA GAGCCCCGTC TCCTGGAGCT CGAGCAGGAC
    CCACTCAACT GGAGAGAACT TGCTTCCAGT GAAAGCCTGC ATAAGCTGAG CAAGAAGGAA
    GCCAAAAGAC AAGAAGTCAT AAATGAGCTG TTTGTGACAG AACAAGCACA CGTCCGTATG
    CTGAGTGTAT TGCAGACAGT GTTTTCTCGT CCACTTGAGA AGCAAGAGTA TATGACTGCA
    AATGAGGTGG CCACCATCTT CTCCAGCCTG GATGACATCA TAGAGATGCA CTATGTTTTC
    TATGAGAACC TGAAGAAACT GCGTGAAGAA GATGAATACA TTGTCAAAAC TATCAGCACA
    CCACTTCTCA ACAGATTCAG TGGATCTGAA GGAGAATGGT TTCAAAAGCT CACAGCACGC
    TTCTGTAGCT ACCAGTCCTG GGCTCTGGAG CAGATCAAGA GCAGGCAGAA GAAGGACCCC
    CGATTCAATG CTTTTATACT GGAAGCAGAG AGTAAGCCAC AGTGTCGGAG GCTACAGCTA
    AAGGATATCA TACCCATAGA GATGCAGAGA CTAACAAAAT ACCCTCTTCT GCTGGAAAAC
    ATTGCTAAGA ACACAGAGGA TGCAACAGAA AAAGAGAAAA TTAACCAAGC AGCAGAATCC
    TGCCGTAAGA TCCTCAACCA TGTCAATGAA GAAGTCAAAC AAATGGAGAA CCTGCTGATT
    TTGAAAGACT ACCAGCGTCG ACTGGATACC TCAGGACTGA AACCCAGCAA TGAACTCTAC
    AGTGAGTACA AGACCATTGA TCTGACCAAC CGAACGATGC TGTTTGAAGG ACCTCTGACA
    TGGAGGGTGA CCAAAGAGAA AGCAATCGAT GTGCACTGTG TGTTGCTGTC AGACCTGCTG
    GTCTTGTTTC AGAAGCAGGA TGATAAGATG CTTTTGAAGT GTCAGAGCAA AAGCAACATC
    GCCGTTCAGG AAGGCAAGCA GATGCTGAGT CCAATCATCA AGCTGGAGTC TGTTTTCCTC
    AGAGAGGTGG CCACTGACCC GAAGGCCCTG TATGTTATCT TCACCTGGGA CAGTGGAGCA
    CAGATCTATG AACTGGTGGC TCAGTCTGTC GGGGAGAGGA AAAACTGGAC AGAGGCGATT
    AAGTCTGCGG TTGATGAGCT GAAGAAGGCA TCAAAGAGCA ACACCAAACT GGAAAGGAAT
    AGCAGGGCTG GCAGTGTGCC AAACAACATT GCTGTACCTC TATATGGCCC AGCTCATCCT
    CCTCTTAGCC CCACTGAAAA TGGAGGTGAA CTGCTGAAAA ATCGCGTCGG GCGATTTAGT
    GAGAAGATTC GAGAAAGTGA TCCTCCACTC ATCGACTATC TAGCAGCCAA CGGCTTTGAC
    CTGCTCACCC ACAGCCACAC ACCTCCGGAG AAATCTGCTA CTGTTGCTTT GGATGAAGTC
    ATGTGTTTGA AGAGGCTATT GGTTGGGAGT ATTAGCCTGT CAGATGATGC TCAGACTCTT
    GGGGAAAACG GTGGGATGGC ACCTGAAAGT CAGGATGCAG ATGGAACTCA ACTGTCAGTG
    GAGGAATCGA GCACAGAAGC AGGTCCTGAA GTAGAAGAGC ATGAGGAAGA TGTAGAGAAT
    AAAGGAGAAG AGGAGAAAGG TATCAGTGCT CCTCTCGTGC TGTCTGAGGA GCGCATGGAG
    GAGGTGCAGA TGAGACTGCA GGCTCTGGAG GAGCAGCTGA AGAGATTGAG GTTGGTTGAG
    GAGGACCATT ATAAATTACA AGAAGCTCTT GCTAAATACA CACTTCAGGG GGGCCATTAC
    AATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149247
    Clone ID Related Accession (Same CDS sequence) XM_021466703.1
    Accession Version XM_021466703.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3162bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 1 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCTGTTGT TCTCCATTCT TGTAGGAGCT CGGTTTGCTG GTGTTGGTAG GACCTGCGAC 
    AGCATGGATT ACGAAGACGC CCATAATGGG CGCGGGCTGT CAGGTGCTCA GGGTTTAAAT
    CCAGTCATGA ACATAATCGG AGCAGAGGAT GAAGACTTTG AGAATGATGA GCAGCCTATG
    GTGGACGATC AGTGCATTCA CTTTAACAGT ATTGAGCTGT TGAAGTCCAG GCCCACCCAC
    CTGCTGGTGT TCCTTCATCA TGTCATGCTA CAGTTTGACT GTGCTCCTGT GCTATGCTAC
    CTTCATGCTG ACCTCATGAA GAACCTCAAT ACCAAAGACA CCAAGAAGCA CTTTGGGGAA
    TTCTACAACA CTTTCCTGGA GAAAGGAGCG ATTCTCAAAG TATCTGTACC AAGTAACATC
    GCCCCTGAAA TAGATCGCTT GCGTCCAGAA CTGATCAATG AAGAGCAGCA AAAACGTTAC
    GCTAATGAAA TACAGTTTGC GCAGAGTCCA GAGATACTGC GACAGCTGGA AGACTTCAGG
    CAAAAGAGGA TGATGGGAAT GACACCTAAT GAGCGGGAAT TAAATGATGT GGAATCCCAT
    CGACCCACTG ATCGCATCCC TATGGACATG AAGGAGAAGG CTGTCGCTGA ATCCCTGCTG
    GAAAAGATGG TTGAGGCCAA CCCCTCAATT GTTCCTGACA AGGACAAAAG TAACTCCATC
    TTTGGAGCAG TGGCTTATTA TATGAAGTAT CTTGGAGTCA AGACCAGAGC TTTTGACAAC
    AAGAAGTCCA AGTCAGGATG GCGGCCCTCG GTTCCATCTA TGCTGAAGCC ATGGTCAGTC
    AACAAGCAGA TTAAACCAGT CAAACCGCCT GGAATCGGCT ATGACGGGCG AAAAACTGGT
    ATTACCGTGT CAGGGAGCAC TCATGGGTCA GAGAGCAGTG AAGAGTTACC CATCAGTGTG
    AGTGTGACTC CCAGCCCTGA CTTTCAAACT GATACAGTAG GTATCATCAA CAATCGCACA
    GACCCCAAAC CAGCCTCTGA GGGTGGAGAA GTGTCTCCAG TCAGCACTGG TGGGACTGTG
    TCCATCTGTG AACCTCTCGC TGTCATTGAT GTCCCTCCAG AAGACATTCA GGACAGCGGC
    AGAAAGAAGC CAAGGAATGT GCGTCGCAGT GAAAGCCTTT GTGTGGAGCG GCGTCACTCT
    CGTCGTGGTG GCTCTACTCG GGCCAAGCAG TCTCGTTCCC GCAGCGACGT GGACCTCCAG
    TCAACTTCCA CATCCCATCC CCTCTCACCC TCCCCACAGC ATTCAGTCTC CGTGGAAGGT
    GACAAAACAC CGGAAATCGC TCTCCTCACT CCCACTTTTG TCCCGGCCCC TCAGCAAGAA
    GAGATAGAGC CCCGTCTCCT GGAGCTCGAG CAGGACCCAC TCAACTGGAG AGAACTTGCT
    TCCAGTGAAA GCCTGCATAA GCTGAGCAAG AAGGAAGCCA AAAGACAAGA AGTCATAAAT
    GAGCTGTTTG TGACAGAACA AGCACACGTC CGTATGCTGA GTGTATTGCA GACAGTGTTT
    TCTCGTCCAC TTGAGAAGCA AGAGTATATG ACTGCAAATG AGGTGGCCAC CATCTTCTCC
    AGCCTGGATG ACATCATAGA GATGCACTAT GTTTTCTATG AGAACCTGAA GAAACTGCGT
    GAAGAAGATG AATACATTGT CAAAACTATC AGCACACCAC TTCTCAACAG ATTCAGTGGA
    TCTGAAGGAG AATGGTTTCA AAAGCTCACA GCACGCTTCT GTAGCTACCA GTCCTGGGCT
    CTGGAGCAGA TCAAGAGCAG GCAGAAGAAG GACCCCCGAT TCAATGCTTT TATACTGGAA
    GCAGAGAGTA AGCCACAGTG TCGGAGGCTA CAGCTAAAGG ATATCATACC CATAGAGATG
    CAGAGACTAA CAAAATACCC TCTTCTGCTG GAAAACATTG CTAAGAACAC AGAGGATGCA
    ACAGAAAAAG AGAAAATTAA CCAAGCAGCA GAATCCTGCC GTAAGATCCT CAACCATGTC
    AATGAAGAAG TCAAACAAAT GGAGAACCTG CTGATTTTGA AAGACTACCA GCGTCGACTG
    GATACCTCAG GACTGAAACC CAGCAATGAA CTCTACAGTG AGTACAAGAC CATTGATCTG
    ACCAACCGAA CGATGCTGTT TGAAGGACCT CTGACATGGA GGGTGACCAA AGAGAAAGCA
    ATCGATGTGC ACTGTGTGTT GCTGTCAGAC CTGCTGGTCT TGTTTCAGAA GCAGGATGAT
    AAGATGCTTT TGAAGTGTCA GAGCAAAAGC AACATCGCCG TTCAGGAAGG CAAGCAGATG
    CTGAGTCCAA TCATCAAGCT GGAGTCTGTT TTCCTCAGAG AGGTGGCCAC TGACCCGAAG
    GCCCTGTATG TTATCTTCAC CTGGGACAGT GGAGCACAGA TCTATGAACT GGTGGCTCAG
    TCTGTCGGGG AGAGGAAAAA CTGGACAGAG GCGATTAAGT CTGCGGTTGA TGAGCTGAAG
    AAGGCATCAA AGAGCAACAC CAAACTGGAA AGGAATAGCA GGGCTGGCAG TGTGCCAAAC
    AACATTGCTG TACCTCTATA TGGCCCAGCT CATCCTCCTC TTAGCCCCAC TGAAAATGGA
    GGTGAACTGC TGAAAAATCG CGTCGGGCGA TTTAGTGAGA AGATTCGAGA AAGTGATCCT
    CCACTCATCG ACTATCTAGC AGCCAACGGC TTTGACCTGC TCACCCACAG CCACACACCT
    CCGGAGAAAT CTGCTACTGT TGCTTTGGAT GAAGTCATGT GTTTGAAGAG GCTATTGGTT
    GGGAGTATTA GCCTGTCAGA TGATGCTCAG ACTCTTGGGG AAAACGGTGG GATGGCACCT
    GAAAGTCAGG ATGCAGATGG AACTCAACTG TCAGTGGAGG AATCGAGCAC AGAAGCAGGT
    CCTGAAGTAG AAGAGCATGA GGAAGATGTA GAGAATAAAG GAGAAGAGGA GAAAGGTATC
    AGTGCTCCTC TCGTGCTGTC TGAGGAGCGC ATGGAGGAGG TGCAGATGAG ACTGCAGGCT
    CTGGAGGAGC AGCTGAAGAG ATTGAGGTTG GTTGAGGAGG ACCATTATAA ATTACAAGAA
    GCTCTTGCTA AATACACACT TCAGGGGGGC CATTACAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322378.1
    CDS262..3423
    Translation

    Target ORF information:

    RefSeq Version XM_021466703.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 1b (arhgef1b), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466703.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGCTGTTGT TCTCCATTCT TGTAGGAGCT CGGTTTGCTG GTGTTGGTAG GACCTGCGAC 
    AGCATGGATT ACGAAGACGC CCATAATGGG CGCGGGCTGT CAGGTGCTCA GGGTTTAAAT
    CCAGTCATGA ACATAATCGG AGCAGAGGAT GAAGACTTTG AGAATGATGA GCAGCCTATG
    GTGGACGATC AGTGCATTCA CTTTAACAGT ATTGAGCTGT TGAAGTCCAG GCCCACCCAC
    CTGCTGGTGT TCCTTCATCA TGTCATGCTA CAGTTTGACT GTGCTCCTGT GCTATGCTAC
    CTTCATGCTG ACCTCATGAA GAACCTCAAT ACCAAAGACA CCAAGAAGCA CTTTGGGGAA
    TTCTACAACA CTTTCCTGGA GAAAGGAGCG ATTCTCAAAG TATCTGTACC AAGTAACATC
    GCCCCTGAAA TAGATCGCTT GCGTCCAGAA CTGATCAATG AAGAGCAGCA AAAACGTTAC
    GCTAATGAAA TACAGTTTGC GCAGAGTCCA GAGATACTGC GACAGCTGGA AGACTTCAGG
    CAAAAGAGGA TGATGGGAAT GACACCTAAT GAGCGGGAAT TAAATGATGT GGAATCCCAT
    CGACCCACTG ATCGCATCCC TATGGACATG AAGGAGAAGG CTGTCGCTGA ATCCCTGCTG
    GAAAAGATGG TTGAGGCCAA CCCCTCAATT GTTCCTGACA AGGACAAAAG TAACTCCATC
    TTTGGAGCAG TGGCTTATTA TATGAAGTAT CTTGGAGTCA AGACCAGAGC TTTTGACAAC
    AAGAAGTCCA AGTCAGGATG GCGGCCCTCG GTTCCATCTA TGCTGAAGCC ATGGTCAGTC
    AACAAGCAGA TTAAACCAGT CAAACCGCCT GGAATCGGCT ATGACGGGCG AAAAACTGGT
    ATTACCGTGT CAGGGAGCAC TCATGGGTCA GAGAGCAGTG AAGAGTTACC CATCAGTGTG
    AGTGTGACTC CCAGCCCTGA CTTTCAAACT GATACAGTAG GTATCATCAA CAATCGCACA
    GACCCCAAAC CAGCCTCTGA GGGTGGAGAA GTGTCTCCAG TCAGCACTGG TGGGACTGTG
    TCCATCTGTG AACCTCTCGC TGTCATTGAT GTCCCTCCAG AAGACATTCA GGACAGCGGC
    AGAAAGAAGC CAAGGAATGT GCGTCGCAGT GAAAGCCTTT GTGTGGAGCG GCGTCACTCT
    CGTCGTGGTG GCTCTACTCG GGCCAAGCAG TCTCGTTCCC GCAGCGACGT GGACCTCCAG
    TCAACTTCCA CATCCCATCC CCTCTCACCC TCCCCACAGC ATTCAGTCTC CGTGGAAGGT
    GACAAAACAC CGGAAATCGC TCTCCTCACT CCCACTTTTG TCCCGGCCCC TCAGCAAGAA
    GAGATAGAGC CCCGTCTCCT GGAGCTCGAG CAGGACCCAC TCAACTGGAG AGAACTTGCT
    TCCAGTGAAA GCCTGCATAA GCTGAGCAAG AAGGAAGCCA AAAGACAAGA AGTCATAAAT
    GAGCTGTTTG TGACAGAACA AGCACACGTC CGTATGCTGA GTGTATTGCA GACAGTGTTT
    TCTCGTCCAC TTGAGAAGCA AGAGTATATG ACTGCAAATG AGGTGGCCAC CATCTTCTCC
    AGCCTGGATG ACATCATAGA GATGCACTAT GTTTTCTATG AGAACCTGAA GAAACTGCGT
    GAAGAAGATG AATACATTGT CAAAACTATC AGCACACCAC TTCTCAACAG ATTCAGTGGA
    TCTGAAGGAG AATGGTTTCA AAAGCTCACA GCACGCTTCT GTAGCTACCA GTCCTGGGCT
    CTGGAGCAGA TCAAGAGCAG GCAGAAGAAG GACCCCCGAT TCAATGCTTT TATACTGGAA
    GCAGAGAGTA AGCCACAGTG TCGGAGGCTA CAGCTAAAGG ATATCATACC CATAGAGATG
    CAGAGACTAA CAAAATACCC TCTTCTGCTG GAAAACATTG CTAAGAACAC AGAGGATGCA
    ACAGAAAAAG AGAAAATTAA CCAAGCAGCA GAATCCTGCC GTAAGATCCT CAACCATGTC
    AATGAAGAAG TCAAACAAAT GGAGAACCTG CTGATTTTGA AAGACTACCA GCGTCGACTG
    GATACCTCAG GACTGAAACC CAGCAATGAA CTCTACAGTG AGTACAAGAC CATTGATCTG
    ACCAACCGAA CGATGCTGTT TGAAGGACCT CTGACATGGA GGGTGACCAA AGAGAAAGCA
    ATCGATGTGC ACTGTGTGTT GCTGTCAGAC CTGCTGGTCT TGTTTCAGAA GCAGGATGAT
    AAGATGCTTT TGAAGTGTCA GAGCAAAAGC AACATCGCCG TTCAGGAAGG CAAGCAGATG
    CTGAGTCCAA TCATCAAGCT GGAGTCTGTT TTCCTCAGAG AGGTGGCCAC TGACCCGAAG
    GCCCTGTATG TTATCTTCAC CTGGGACAGT GGAGCACAGA TCTATGAACT GGTGGCTCAG
    TCTGTCGGGG AGAGGAAAAA CTGGACAGAG GCGATTAAGT CTGCGGTTGA TGAGCTGAAG
    AAGGCATCAA AGAGCAACAC CAAACTGGAA AGGAATAGCA GGGCTGGCAG TGTGCCAAAC
    AACATTGCTG TACCTCTATA TGGCCCAGCT CATCCTCCTC TTAGCCCCAC TGAAAATGGA
    GGTGAACTGC TGAAAAATCG CGTCGGGCGA TTTAGTGAGA AGATTCGAGA AAGTGATCCT
    CCACTCATCG ACTATCTAGC AGCCAACGGC TTTGACCTGC TCACCCACAG CCACACACCT
    CCGGAGAAAT CTGCTACTGT TGCTTTGGAT GAAGTCATGT GTTTGAAGAG GCTATTGGTT
    GGGAGTATTA GCCTGTCAGA TGATGCTCAG ACTCTTGGGG AAAACGGTGG GATGGCACCT
    GAAAGTCAGG ATGCAGATGG AACTCAACTG TCAGTGGAGG AATCGAGCAC AGAAGCAGGT
    CCTGAAGTAG AAGAGCATGA GGAAGATGTA GAGAATAAAG GAGAAGAGGA GAAAGGTATC
    AGTGCTCCTC TCGTGCTGTC TGAGGAGCGC ATGGAGGAGG TGCAGATGAG ACTGCAGGCT
    CTGGAGGAGC AGCTGAAGAG ATTGAGGTTG GTTGAGGAGG ACCATTATAA ATTACAAGAA
    GCTCTTGCTA AATACACACT TCAGGGGGGC CATTACAATT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149248
    Clone ID Related Accession (Same CDS sequence) XM_021466704.1
    Accession Version XM_021466704.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3081bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 1 isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCTGTTGT TCTCCATTCT TGTAGGAGCT CGGTTTGCTG GTGTTGGTAG GACCTGCGAC 
    AGCATGGATT ACGAAGACGC CCATAATGGG CGCGGGCTGT CAGGTGCTCA GGGTTTAAAT
    CCAGTCATGA ACATAATCGG AGCAGAGGAT GAAGACTTTG AGAATGATGA GCAGCCTATG
    GTGGACGATC AGTGCATTCA CTTTAACAGT ATTGAGCTGT TGAAGTCCAG GCCCACCCAC
    CTGCTGGTGT TCCTTCATCA TGTCATGCTA CAGTTTGACT GTGCTCCTGT GCTATGCTAC
    CTTCATGCTG ACCTCATGAA GAACCTCAAT ACCAAAGACA CCAAGAAGCA CTTTGGGGAA
    TTCTACAACA CTTTCCTGGA GAAAGGAGCG ATTCTCAAAG TATCTGTACC AAGTAACATC
    GCCCCTGAAA TAGATCGCTT GCGTCCAGAA CTGATCAATG AAGAGCAGCA AAAACGTTAC
    GCTAATGAAA TACAGTTTGC GCAGAGTCCA GAGATACTGC GACAGCTGGA AGACTTCAGG
    CAAAAGAGGA TGATGGGAAT GACACCTAAT GAGCGGGAAT TAAATGATGT GGAATCCCAT
    CGACCCACTG ATCGCATCCC TATGGACATG AAGGAGAAGG CTGTCGCTGA ATCCCTGCTG
    GAAAAGATGG TTGAGGCCAA CCCCTCAATT GTTCCTGACA AGGACAAAAG TAACTCCATC
    TTTGGAGCAG TGGCTTATTA TATGAAGTAT CTTGGAGTCA AGACCAGAGC TTTTGACAAC
    AAGAAGTCCA AGTCAGGATG GCGGCCCTCG GTTCCATCTA TGCTGAAGCC ATGGTCAGTC
    AACAAGCAGA TTAAACCAGT CAAACCGCCT GGAATCGGCT ATGACGGTAA GCAAAGTAGA
    TTGGACTTTG AAGCAAAGCA AAACAAACAA CCTCCTCATC GAGTGTCTGT TAGTGATAGT
    GTCACCCCTC CAGGGCGAAA AACTGGTATT ACCGTGTCAG GGAGCACTCA TGGGTCAGAG
    AGCAGTGAAG AGTTACCCAT CAGTGTGAGT GTGACTCCCA GCCCTGACTT TCAAACTGAT
    ACAGTAGGTA TCATCAACAA TCGCACAGAC CCCAAACCAG CCTCTGAGGG TGGAGAAGTG
    TCTCCAGTCA GCACTGGTGG GACTGTGTCC ATCTGTGAAC CTCTCGCTGT CATTGATGTC
    CCTCCAGAAG ACATTCAGGA CAGCGGCAGC GTGGAAGGTG ACAAAACACC GGAAATCGCT
    CTCCTCACTC CCACTTTTGT CCCGGCCCCT CAGCAAGAAG AGATAGAGCC CCGTCTCCTG
    GAGCTCGAGC AGGACCCACT CAACTGGAGA GAACTTGCTT CCAGTGAAAG CCTGCATAAG
    CTGAGCAAGA AGGAAGCCAA AAGACAAGAA GTCATAAATG AGCTGTTTGT GACAGAACAA
    GCACACGTCC GTATGCTGAG TGTATTGCAG ACAGTGTTTT CTCGTCCACT TGAGAAGCAA
    GAGTATATGA CTGCAAATGA GGTGGCCACC ATCTTCTCCA GCCTGGATGA CATCATAGAG
    ATGCACTATG TTTTCTATGA GAACCTGAAG AAACTGCGTG AAGAAGATGA ATACATTGTC
    AAAACTATCA GCACACCACT TCTCAACAGA TTCAGTGGAT CTGAAGGAGA ATGGTTTCAA
    AAGCTCACAG CACGCTTCTG TAGCTACCAG TCCTGGGCTC TGGAGCAGAT CAAGAGCAGG
    CAGAAGAAGG ACCCCCGATT CAATGCTTTT ATACTGGAAG CAGAGAGTAA GCCACAGTGT
    CGGAGGCTAC AGCTAAAGGA TATCATACCC ATAGAGATGC AGAGACTAAC AAAATACCCT
    CTTCTGCTGG AAAACATTGC TAAGAACACA GAGGATGCAA CAGAAAAAGA GAAAATTAAC
    CAAGCAGCAG AATCCTGCCG TAAGATCCTC AACCATGTCA ATGAAGAAGT CAAACAAATG
    GAGAACCTGC TGATTTTGAA AGACTACCAG CGTCGACTGG ATACCTCAGG ACTGAAACCC
    AGCAATGAAC TCTACAGTGA GTACAAGACC ATTGATCTGA CCAACCGAAC GATGCTGTTT
    GAAGGACCTC TGACATGGAG GGTGACCAAA GAGAAAGCAA TCGATGTGCA CTGTGTGTTG
    CTGTCAGACC TGCTGGTCTT GTTTCAGAAG CAGGATGATA AGATGCTTTT GAAGTGTCAG
    AGCAAAAGCA ACATCGCCGT TCAGGAAGGC AAGCAGATGC TGAGTCCAAT CATCAAGCTG
    GAGTCTGTTT TCCTCAGAGA GGTGGCCACT GACCCGAAGG CCCTGTATGT TATCTTCACC
    TGGGACAGTG GAGCACAGAT CTATGAACTG GTGGCTCAGT CTGTCGGGGA GAGGAAAAAC
    TGGACAGAGG CGATTAAGTC TGCGGTTGAT GAGCTGAAGA AGGCATCAAA GAGCAACACC
    AAACTGGAAA GGAATAGCAG GGCTGGCAGT GTGCCAAACA ACATTGCTGT ACCTCTATAT
    GGCCCAGCTC ATCCTCCTCT TAGCCCCACT GAAAATGGAG GTGAACTGCT GAAAAATCGC
    GTCGGGCGAT TTAGTGAGAA GATTCGAGAA AGTGATCCTC CACTCATCGA CTATCTAGCA
    GCCAACGGCT TTGACCTGCT CACCCACAGC CACACACCTC CGGAGAAATC TGCTACTGTT
    GCTTTGGATG AAGTCATGTG TTTGAAGAGG CTATTGGTTG GGAGTATTAG CCTGTCAGAT
    GATGCTCAGA CTCTTGGGGA AAACGGTGGG ATGGCACCTG AAAGTCAGGA TGCAGATGGA
    ACTCAACTGT CAGTGGAGGA ATCGAGCACA GAAGCAGGTC CTGAAGTAGA AGAGCATGAG
    GAAGATGTAG AGAATAAAGG AGAAGAGGAG AAAGGTATCA GTGCTCCTCT CGTGCTGTCT
    GAGGAGCGCA TGGAGGAGGT GCAGATGAGA CTGCAGGCTC TGGAGGAGCA GCTGAAGAGA
    TTGAGGTTGG TTGAGGAGGA CCATTATAAA TTACAAGAAG CTCTTGCTAA ATACACACTT
    CAGGGGGGCC ATTACAATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322379.1
    CDS259..3339
    Translation

    Target ORF information:

    RefSeq Version XM_021466704.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 1b (arhgef1b), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466704.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    ATGCTGTTGT TCTCCATTCT TGTAGGAGCT CGGTTTGCTG GTGTTGGTAG GACCTGCGAC 
    AGCATGGATT ACGAAGACGC CCATAATGGG CGCGGGCTGT CAGGTGCTCA GGGTTTAAAT
    CCAGTCATGA ACATAATCGG AGCAGAGGAT GAAGACTTTG AGAATGATGA GCAGCCTATG
    GTGGACGATC AGTGCATTCA CTTTAACAGT ATTGAGCTGT TGAAGTCCAG GCCCACCCAC
    CTGCTGGTGT TCCTTCATCA TGTCATGCTA CAGTTTGACT GTGCTCCTGT GCTATGCTAC
    CTTCATGCTG ACCTCATGAA GAACCTCAAT ACCAAAGACA CCAAGAAGCA CTTTGGGGAA
    TTCTACAACA CTTTCCTGGA GAAAGGAGCG ATTCTCAAAG TATCTGTACC AAGTAACATC
    GCCCCTGAAA TAGATCGCTT GCGTCCAGAA CTGATCAATG AAGAGCAGCA AAAACGTTAC
    GCTAATGAAA TACAGTTTGC GCAGAGTCCA GAGATACTGC GACAGCTGGA AGACTTCAGG
    CAAAAGAGGA TGATGGGAAT GACACCTAAT GAGCGGGAAT TAAATGATGT GGAATCCCAT
    CGACCCACTG ATCGCATCCC TATGGACATG AAGGAGAAGG CTGTCGCTGA ATCCCTGCTG
    GAAAAGATGG TTGAGGCCAA CCCCTCAATT GTTCCTGACA AGGACAAAAG TAACTCCATC
    TTTGGAGCAG TGGCTTATTA TATGAAGTAT CTTGGAGTCA AGACCAGAGC TTTTGACAAC
    AAGAAGTCCA AGTCAGGATG GCGGCCCTCG GTTCCATCTA TGCTGAAGCC ATGGTCAGTC
    AACAAGCAGA TTAAACCAGT CAAACCGCCT GGAATCGGCT ATGACGGTAA GCAAAGTAGA
    TTGGACTTTG AAGCAAAGCA AAACAAACAA CCTCCTCATC GAGTGTCTGT TAGTGATAGT
    GTCACCCCTC CAGGGCGAAA AACTGGTATT ACCGTGTCAG GGAGCACTCA TGGGTCAGAG
    AGCAGTGAAG AGTTACCCAT CAGTGTGAGT GTGACTCCCA GCCCTGACTT TCAAACTGAT
    ACAGTAGGTA TCATCAACAA TCGCACAGAC CCCAAACCAG CCTCTGAGGG TGGAGAAGTG
    TCTCCAGTCA GCACTGGTGG GACTGTGTCC ATCTGTGAAC CTCTCGCTGT CATTGATGTC
    CCTCCAGAAG ACATTCAGGA CAGCGGCAGC GTGGAAGGTG ACAAAACACC GGAAATCGCT
    CTCCTCACTC CCACTTTTGT CCCGGCCCCT CAGCAAGAAG AGATAGAGCC CCGTCTCCTG
    GAGCTCGAGC AGGACCCACT CAACTGGAGA GAACTTGCTT CCAGTGAAAG CCTGCATAAG
    CTGAGCAAGA AGGAAGCCAA AAGACAAGAA GTCATAAATG AGCTGTTTGT GACAGAACAA
    GCACACGTCC GTATGCTGAG TGTATTGCAG ACAGTGTTTT CTCGTCCACT TGAGAAGCAA
    GAGTATATGA CTGCAAATGA GGTGGCCACC ATCTTCTCCA GCCTGGATGA CATCATAGAG
    ATGCACTATG TTTTCTATGA GAACCTGAAG AAACTGCGTG AAGAAGATGA ATACATTGTC
    AAAACTATCA GCACACCACT TCTCAACAGA TTCAGTGGAT CTGAAGGAGA ATGGTTTCAA
    AAGCTCACAG CACGCTTCTG TAGCTACCAG TCCTGGGCTC TGGAGCAGAT CAAGAGCAGG
    CAGAAGAAGG ACCCCCGATT CAATGCTTTT ATACTGGAAG CAGAGAGTAA GCCACAGTGT
    CGGAGGCTAC AGCTAAAGGA TATCATACCC ATAGAGATGC AGAGACTAAC AAAATACCCT
    CTTCTGCTGG AAAACATTGC TAAGAACACA GAGGATGCAA CAGAAAAAGA GAAAATTAAC
    CAAGCAGCAG AATCCTGCCG TAAGATCCTC AACCATGTCA ATGAAGAAGT CAAACAAATG
    GAGAACCTGC TGATTTTGAA AGACTACCAG CGTCGACTGG ATACCTCAGG ACTGAAACCC
    AGCAATGAAC TCTACAGTGA GTACAAGACC ATTGATCTGA CCAACCGAAC GATGCTGTTT
    GAAGGACCTC TGACATGGAG GGTGACCAAA GAGAAAGCAA TCGATGTGCA CTGTGTGTTG
    CTGTCAGACC TGCTGGTCTT GTTTCAGAAG CAGGATGATA AGATGCTTTT GAAGTGTCAG
    AGCAAAAGCA ACATCGCCGT TCAGGAAGGC AAGCAGATGC TGAGTCCAAT CATCAAGCTG
    GAGTCTGTTT TCCTCAGAGA GGTGGCCACT GACCCGAAGG CCCTGTATGT TATCTTCACC
    TGGGACAGTG GAGCACAGAT CTATGAACTG GTGGCTCAGT CTGTCGGGGA GAGGAAAAAC
    TGGACAGAGG CGATTAAGTC TGCGGTTGAT GAGCTGAAGA AGGCATCAAA GAGCAACACC
    AAACTGGAAA GGAATAGCAG GGCTGGCAGT GTGCCAAACA ACATTGCTGT ACCTCTATAT
    GGCCCAGCTC ATCCTCCTCT TAGCCCCACT GAAAATGGAG GTGAACTGCT GAAAAATCGC
    GTCGGGCGAT TTAGTGAGAA GATTCGAGAA AGTGATCCTC CACTCATCGA CTATCTAGCA
    GCCAACGGCT TTGACCTGCT CACCCACAGC CACACACCTC CGGAGAAATC TGCTACTGTT
    GCTTTGGATG AAGTCATGTG TTTGAAGAGG CTATTGGTTG GGAGTATTAG CCTGTCAGAT
    GATGCTCAGA CTCTTGGGGA AAACGGTGGG ATGGCACCTG AAAGTCAGGA TGCAGATGGA
    ACTCAACTGT CAGTGGAGGA ATCGAGCACA GAAGCAGGTC CTGAAGTAGA AGAGCATGAG
    GAAGATGTAG AGAATAAAGG AGAAGAGGAG AAAGGTATCA GTGCTCCTCT CGTGCTGTCT
    GAGGAGCGCA TGGAGGAGGT GCAGATGAGA CTGCAGGCTC TGGAGGAGCA GCTGAAGAGA
    TTGAGGTTGG TTGAGGAGGA CCATTATAAA TTACAAGAAG CTCTTGCTAA ATACACACTT
    CAGGGGGGCC ATTACAATTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa149249
    Clone ID Related Accession (Same CDS sequence) XM_021466705.1
    Accession Version XM_021466705.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3009bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 1 isoform X9
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCATGCT ACAGTTTGAC TGTGCTCCTG TGCCAATGTG ATCTACCCCA GCTATGCTAC 
    CTTCATGCTG ACCTCATGAA GAACCTCAAT ACCAAAGACA CCAAGAAGCA CTTTGGGGAA
    TTCTACAACA CTTTCCTGGA GAAAGGAGCG ATTCTCAAAG TATCTGTACC AAGTAACATC
    GCCCCTGAAA TAGATCGCTT GCGTCCAGAA CTGATCAATG AAGAGCAGCA AAAACGTTAC
    GCTAATGAAA TACAGTTTGC GCAGAGTCCA GAGATACTGC GACAGCTGGA AGACTTCAGG
    CAAAAGAGGA TGATGGGAAT GACACCTAAT GAGCGGGAAT TAAATGATGT GGAATCCCAT
    CGACCCACTG ATCGCATCCC TATGGACATG AAGGAGAAGG CTGTCGCTGA ATCCCTGCTG
    GAAAAGATGG TTGAGGCCAA CCCCTCAATT GTTCCTGACA AGGACAAAAG TAACTCCATC
    TTTGGAGCAG TGGCTTATTA TATGAAGTAT CTTGGAGTCA AGACCAGAGC TTTTGACAAC
    AAGAAGTCCA AGTCAGGATG GCGGCCCTCG GTTCCATCTA TGCTGAAGCC ATGGTCAGTC
    AACAAGCAGA TTAAACCAGT CAAACCGCCT GGAATCGGCT ATGACGGTAA GCAAAGTAGA
    TTGGACTTTG AAGCAAAGCA AAACAAACAA CCTCCTCATC GAGTGTCTGT TAGTGATAGT
    GTCACCCCTC CAGGGCGAAA AACTGGTATT ACCGTGTCAG GGAGCACTCA TGGGTCAGAG
    AGCAGTGAAG AGTTACCCAT CAGTGTGAGT GTGACTCCCA GCCCTGACTT TCAAACTGAT
    ACAGTAGGTA TCATCAACAA TCGCACAGAC CCCAAACCAG CCTCTGAGGG TGGAGAAGTG
    TCTCCAGTCA GCACTGGTGG GACTGTGTCC ATCTGTGAAC CTCTCGCTGT CATTGATGTC
    CCTCCAGAAG ACATTCAGGA CAGCGGCAGA AAGAAGCCAA GGAATGTGCG TCGCAGTGAA
    AGCCTTTGTG TGGAGCGGCG TCACTCTCGT CGTGGTGGCT CTACTCGGGC CAAGCAGTCT
    CGTTCCCGCA GCGACGTGGA CCTCCAGTCA ACTTCCACAT CCCATCCCCT CTCACCCTCC
    CCACAGCATT CAGTCTCCGT GGAAGGTGAC AAAACACCGG AAATCGCTCT CCTCACTCCC
    ACTTTTGTCC CGGCCCCTCA GCAAGAAGAG ATAGAGCCCC GTCTCCTGGA GCTCGAGCAG
    GACCCACTCA ACTGGAGAGA ACTTGCTTCC AGTGAAAGCC TGCATAAGCT GAGCAAGAAG
    GAAGCCAAAA GACAAGAAGT CATAAATGAG CTGTTTGTGA CAGAACAAGC ACACGTCCGT
    ATGCTGAGTG TATTGCAGAC AGTGTTTTCT CGTCCACTTG AGAAGCAAGA GTATATGACT
    GCAAATGAGG TGGCCACCAT CTTCTCCAGC CTGGATGACA TCATAGAGAT GCACTATGTT
    TTCTATGAGA ACCTGAAGAA ACTGCGTGAA GAAGATGAAT ACATTGTCAA AACTATCAGC
    ACACCACTTC TCAACAGATT CAGTGGATCT GAAGGAGAAT GGTTTCAAAA GCTCACAGCA
    CGCTTCTGTA GCTACCAGTC CTGGGCTCTG GAGCAGATCA AGAGCAGGCA GAAGAAGGAC
    CCCCGATTCA ATGCTTTTAT ACTGGAAGCA GAGAGTAAGC CACAGTGTCG GAGGCTACAG
    CTAAAGGATA TCATACCCAT AGAGATGCAG AGACTAACAA AATACCCTCT TCTGCTGGAA
    AACATTGCTA AGAACACAGA GGATGCAACA GAAAAAGAGA AAATTAACCA AGCAGCAGAA
    TCCTGCCGTA AGATCCTCAA CCATGTCAAT GAAGAAGTCA AACAAATGGA GAACCTGCTG
    ATTTTGAAAG ACTACCAGCG TCGACTGGAT ACCTCAGGAC TGAAACCCAG CAATGAACTC
    TACAGTGAGT ACAAGACCAT TGATCTGACC AACCGAACGA TGCTGTTTGA AGGACCTCTG
    ACATGGAGGG TGACCAAAGA GAAAGCAATC GATGTGCACT GTGTGTTGCT GTCAGACCTG
    CTGGTCTTGT TTCAGAAGCA GGATGATAAG ATGCTTTTGA AGTGTCAGAG CAAAAGCAAC
    ATCGCCGTTC AGGAAGGCAA GCAGATGCTG AGTCCAATCA TCAAGCTGGA GTCTGTTTTC
    CTCAGAGAGG TGGCCACTGA CCCGAAGGCC CTGTATGTTA TCTTCACCTG GGACAGTGGA
    GCACAGATCT ATGAACTGGT GGCTCAGTCT GTCGGGGAGA GGAAAAACTG GACAGAGGCG
    ATTAAGTCTG CGGTTGATGA GCTGAAGAAG GCATCAAAGA GCAACACCAA ACTGGAAAGG
    AATAGCAGGG CTGGCAGTGT GCCAAACAAC ATTGCTGTAC CTCTATATGG CCCAGCTCAT
    CCTCCTCTTA GCCCCACTGA AAATGGAGGT GAACTGCTGA AAAATCGCGT CGGGCGATTT
    AGTGAGAAGA TTCGAGAAAG TGATCCTCCA CTCATCGACT ATCTAGCAGC CAACGGCTTT
    GACCTGCTCA CCCACAGCCA CACACCTCCG GAGAAATCTG CTACTGTTGC TTTGGATGAA
    GTCATGTGTT TGAAGAGGCT ATTGGTTGGG AGTATTAGCC TGTCAGATGA TGCTCAGACT
    CTTGGGGAAA ACGGTGGGAT GGCACCTGAA AGTCAGGATG CAGATGGAAC TCAACTGTCA
    GTGGAGGAAT CGAGCACAGA AGCAGGTCCT GAAGTAGAAG AGCATGAGGA AGATGTAGAG
    AATAAAGGAG AAGAGGAGAA AGGTATCAGT GCTCCTCTCG TGCTGTCTGA GGAGCGCATG
    GAGGAGGTGC AGATGAGACT GCAGGCTCTG GAGGAGCAGC TGAAGAGATT GAGGTTGGTT
    GAGGAGGACC ATTATAAATT ACAAGAAGCT CTTGCTAAAT ACACACTTCA GGGGGGCCAT
    TACAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322380.1
    CDS170..3178
    Translation

    Target ORF information:

    RefSeq Version XM_021466705.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 1b (arhgef1b), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466705.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGTCATGCT ACAGTTTGAC TGTGCTCCTG TGCCAATGTG ATCTACCCCA GCTATGCTAC 
    CTTCATGCTG ACCTCATGAA GAACCTCAAT ACCAAAGACA CCAAGAAGCA CTTTGGGGAA
    TTCTACAACA CTTTCCTGGA GAAAGGAGCG ATTCTCAAAG TATCTGTACC AAGTAACATC
    GCCCCTGAAA TAGATCGCTT GCGTCCAGAA CTGATCAATG AAGAGCAGCA AAAACGTTAC
    GCTAATGAAA TACAGTTTGC GCAGAGTCCA GAGATACTGC GACAGCTGGA AGACTTCAGG
    CAAAAGAGGA TGATGGGAAT GACACCTAAT GAGCGGGAAT TAAATGATGT GGAATCCCAT
    CGACCCACTG ATCGCATCCC TATGGACATG AAGGAGAAGG CTGTCGCTGA ATCCCTGCTG
    GAAAAGATGG TTGAGGCCAA CCCCTCAATT GTTCCTGACA AGGACAAAAG TAACTCCATC
    TTTGGAGCAG TGGCTTATTA TATGAAGTAT CTTGGAGTCA AGACCAGAGC TTTTGACAAC
    AAGAAGTCCA AGTCAGGATG GCGGCCCTCG GTTCCATCTA TGCTGAAGCC ATGGTCAGTC
    AACAAGCAGA TTAAACCAGT CAAACCGCCT GGAATCGGCT ATGACGGTAA GCAAAGTAGA
    TTGGACTTTG AAGCAAAGCA AAACAAACAA CCTCCTCATC GAGTGTCTGT TAGTGATAGT
    GTCACCCCTC CAGGGCGAAA AACTGGTATT ACCGTGTCAG GGAGCACTCA TGGGTCAGAG
    AGCAGTGAAG AGTTACCCAT CAGTGTGAGT GTGACTCCCA GCCCTGACTT TCAAACTGAT
    ACAGTAGGTA TCATCAACAA TCGCACAGAC CCCAAACCAG CCTCTGAGGG TGGAGAAGTG
    TCTCCAGTCA GCACTGGTGG GACTGTGTCC ATCTGTGAAC CTCTCGCTGT CATTGATGTC
    CCTCCAGAAG ACATTCAGGA CAGCGGCAGA AAGAAGCCAA GGAATGTGCG TCGCAGTGAA
    AGCCTTTGTG TGGAGCGGCG TCACTCTCGT CGTGGTGGCT CTACTCGGGC CAAGCAGTCT
    CGTTCCCGCA GCGACGTGGA CCTCCAGTCA ACTTCCACAT CCCATCCCCT CTCACCCTCC
    CCACAGCATT CAGTCTCCGT GGAAGGTGAC AAAACACCGG AAATCGCTCT CCTCACTCCC
    ACTTTTGTCC CGGCCCCTCA GCAAGAAGAG ATAGAGCCCC GTCTCCTGGA GCTCGAGCAG
    GACCCACTCA ACTGGAGAGA ACTTGCTTCC AGTGAAAGCC TGCATAAGCT GAGCAAGAAG
    GAAGCCAAAA GACAAGAAGT CATAAATGAG CTGTTTGTGA CAGAACAAGC ACACGTCCGT
    ATGCTGAGTG TATTGCAGAC AGTGTTTTCT CGTCCACTTG AGAAGCAAGA GTATATGACT
    GCAAATGAGG TGGCCACCAT CTTCTCCAGC CTGGATGACA TCATAGAGAT GCACTATGTT
    TTCTATGAGA ACCTGAAGAA ACTGCGTGAA GAAGATGAAT ACATTGTCAA AACTATCAGC
    ACACCACTTC TCAACAGATT CAGTGGATCT GAAGGAGAAT GGTTTCAAAA GCTCACAGCA
    CGCTTCTGTA GCTACCAGTC CTGGGCTCTG GAGCAGATCA AGAGCAGGCA GAAGAAGGAC
    CCCCGATTCA ATGCTTTTAT ACTGGAAGCA GAGAGTAAGC CACAGTGTCG GAGGCTACAG
    CTAAAGGATA TCATACCCAT AGAGATGCAG AGACTAACAA AATACCCTCT TCTGCTGGAA
    AACATTGCTA AGAACACAGA GGATGCAACA GAAAAAGAGA AAATTAACCA AGCAGCAGAA
    TCCTGCCGTA AGATCCTCAA CCATGTCAAT GAAGAAGTCA AACAAATGGA GAACCTGCTG
    ATTTTGAAAG ACTACCAGCG TCGACTGGAT ACCTCAGGAC TGAAACCCAG CAATGAACTC
    TACAGTGAGT ACAAGACCAT TGATCTGACC AACCGAACGA TGCTGTTTGA AGGACCTCTG
    ACATGGAGGG TGACCAAAGA GAAAGCAATC GATGTGCACT GTGTGTTGCT GTCAGACCTG
    CTGGTCTTGT TTCAGAAGCA GGATGATAAG ATGCTTTTGA AGTGTCAGAG CAAAAGCAAC
    ATCGCCGTTC AGGAAGGCAA GCAGATGCTG AGTCCAATCA TCAAGCTGGA GTCTGTTTTC
    CTCAGAGAGG TGGCCACTGA CCCGAAGGCC CTGTATGTTA TCTTCACCTG GGACAGTGGA
    GCACAGATCT ATGAACTGGT GGCTCAGTCT GTCGGGGAGA GGAAAAACTG GACAGAGGCG
    ATTAAGTCTG CGGTTGATGA GCTGAAGAAG GCATCAAAGA GCAACACCAA ACTGGAAAGG
    AATAGCAGGG CTGGCAGTGT GCCAAACAAC ATTGCTGTAC CTCTATATGG CCCAGCTCAT
    CCTCCTCTTA GCCCCACTGA AAATGGAGGT GAACTGCTGA AAAATCGCGT CGGGCGATTT
    AGTGAGAAGA TTCGAGAAAG TGATCCTCCA CTCATCGACT ATCTAGCAGC CAACGGCTTT
    GACCTGCTCA CCCACAGCCA CACACCTCCG GAGAAATCTG CTACTGTTGC TTTGGATGAA
    GTCATGTGTT TGAAGAGGCT ATTGGTTGGG AGTATTAGCC TGTCAGATGA TGCTCAGACT
    CTTGGGGAAA ACGGTGGGAT GGCACCTGAA AGTCAGGATG CAGATGGAAC TCAACTGTCA
    GTGGAGGAAT CGAGCACAGA AGCAGGTCCT GAAGTAGAAG AGCATGAGGA AGATGTAGAG
    AATAAAGGAG AAGAGGAGAA AGGTATCAGT GCTCCTCTCG TGCTGTCTGA GGAGCGCATG
    GAGGAGGTGC AGATGAGACT GCAGGCTCTG GAGGAGCAGC TGAAGAGATT GAGGTTGGTT
    GAGGAGGACC ATTATAAATT ACAAGAAGCT CTTGCTAAAT ACACACTTCA GGGGGGCCAT
    TACAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55380
    Clone ID Related Accession (Same CDS sequence) XM_017351352.1
    Accession Version XM_017351352.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3138bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product rho guanine nucleotide exchange factor 1 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## ab initio :: 1% of CDS bases ##RefSeq-Attributes-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGATTACG AAGACGCCCA TAATGGGCGC GGGCTGTCAG GTGCTCAGGG TTTAAATCCA 
    GTCATGAACA TAATCGGAGC AGAGGATGAA GACTTTGAGA ATGATGAGCA GCCTATGGTG
    GACGATCAGT GCATTCACTT TAACAGTATT GAGCTGTTGA AGTCCAGGCC CACCCACCTG
    CTGGTGTTCC TTCATCATGT CATGCTACAG TTTGACTGTG CTCCTGTGCT ATGCTACCTT
    CATGCTGACC TCATGAAGAA CCTCAATACC AAAGACACCA AGAAGCACTT TGGGGAATTC
    TACAACACTT TCCTGGAGAA AGGAGCGATT CTCAAAGTAT CTGTACCAAG TAACATCGCC
    CCTGAAATAG ATCGCTTGCG TCCAGAACTG ATCAATGAAG AGCAGCAAAA ACGTTACGCT
    AATGAAATAC AGTTTGCGCA GAGTCCAGAG ATACTGCGAC AGCTGGAAGA CTTCAGGCAA
    AAGAGGATGA TGGGAATGAC ACCTAATGAG CGGGAATTAA ATGATGTGGA ATCCCATCGA
    CCCACTGATC GCATCCCTAT GGACATGAAG GAGAAGGCTG TCGCTGAATC CCTGCTGGAA
    AAGATGGTTG AGGCCAACCC CTCAATTGTT CCTGACAAGG ACAAAAGTAA CTCCATCTTT
    GGAGCAGTGG CTTATTATAT GAAGTATCTT GGAGTCAAGA CCAGAGCTTT TGACAACAAG
    AAGTCCAAGT CAGGATGGCG GCCCTCGGTT CCATCTATGC TGAAGCCATG GTCAGTCAAC
    AAGCAGATTA AACCAGTCAA ACCGCCTGGA ATCGGCTATG ACGGTAAGCA AAGTAGATTG
    GACTTTGAAG CAAAGCAAAA CAAACAACCT CCTCATCGAG TGTCTGTTAG TGATAGTGTC
    ACCCCTCCAG GGCGAAAAAC TGGTATTACC GTGTCAGGGA GCACTCATGG GTCAGAGAGC
    AGTGAAGAGT TACCCATCAG TGTGAGTGTG ACTCCCAGCC CTGACTTTCA AACTGATACA
    GTAGGTATCA TCAACAATCG CACAGACCCC AAACCAGCCT CTGAGGGTGG AGAAGTGTCT
    CCAGTCAGCA CTGGTGGGAC TGTGTCCATC TGTGAACCTC TCGCTGTCAT TGATGTCCCT
    CCAGAAGACA TTCAGGACAG CGGCAGAAAG AAGCCAAGGA ATGTGCGTCG CAGTGAAAGC
    CTTTGTGTGG AGCGGCGTCA CTCTCGTCGT GGTGGCTCTA CTCGGGCCAA GCAGTCTCGT
    TCCCGCAGCG ACGTGGACCT CCAGTCAACT TCCACATCCC ATCCCCTCTC ACCCTCCCCA
    CAGCATTCAG TCTCCGTGGA AGGTGACAAA ACACCGGAAA TCGCTCTCCT CACTCCCACT
    TTTGTCCCGG CCCCTCAGCA AGAAGAGATA GAGCCCCGTC TCCTGGAGCT CGAGCAGGAC
    CCACTCAACT GGAGAGAACT TGCTTCCAGT GAAAGCCTGC ATAAGCTGAG CAAGAAGGAA
    GCCAAAAGAC AAGAAGTCAT AAATGAGCTG TTTGTGACAG AACAAGCACA CGTCCGTATG
    CTGAGTGTAT TGCAGACAGT GTTTTCTCGT CCACTTGAGA AGCAAGAGTA TATGACTGCA
    AATGAGGTGG CCACCATCTT CTCCAGCCTG GATGACATCA TAGAGATGCA CTATGTTTTC
    TATGAGAACC TGAAGAAACT GCGTGAAGAA GATGAATACA TTGTCAAAAC TATCAGCACA
    CCACTTCTCA ACAGATTCAG TGGATCTGAA GGAGAATGGT TTCAAAAGCT CACAGCACGC
    TTCTGTAGCT ACCAGTCCTG GGCTCTGGAG CAGATCAAGA GCAGGCAGAA GAAGGACCCC
    CGATTCAATG CTTTTATACT GGAAGCAGAG AGTAAGCCAC AGTGTCGGAG GCTACAGCTA
    AAGGATATCA TACCCATAGA GATGCAGAGA CTAACAAAAT ACCCTCTTCT GCTGGAAAAC
    ATTGCTAAGA ACACAGAGGA TGCAACAGAA AAAGAGAAAA TTAACCAAGC AGCAGAATCC
    TGCCGTAAGA TCCTCAACCA TGTCAATGAA GAAGTCAAAC AAATGGAGAA CCTGCTGATT
    TTGAAAGACT ACCAGCGTCG ACTGGATACC TCAGGACTGA AACCCAGCAA TGAACTCTAC
    AGTGAGTACA AGACCATTGA TCTGACCAAC CGAACGATGC TGTTTGAAGG ACCTCTGACA
    TGGAGGGTGA CCAAAGAGAA AGCAATCGAT GTGCACTGTG TGTTGCTGTC AGACCTGCTG
    GTCTTGTTTC AGAAGCAGGA TGATAAGATG CTTTTGAAGT GTCAGAGCAA AAGCAACATC
    GCCGTTCAGG AAGGCAAGCA GATGCTGAGT CCAATCATCA AGCTGGAGTC TGTTTTCCTC
    AGAGAGGTGG CCACTGACCC GAAGGCCCTG TATGTTATCT TCACCTGGGA CAGTGGAGCA
    CAGATCTATG AACTGGTGGC TCAGTCTGTC GGGGAGAGGA AAAACTGGAC AGAGGCGATT
    AAGTCTGCGG TTGATGAGCT GAAGAAGGCA TCAAAGAGCA ACACCAAACT GGAAAGGAAT
    AGCAGGGCTG GCAGTGTGCC AAACAACATT GCTGTACCTC TATATGGCCC AGCTCATCCT
    CCTCTTAGCC CCACTGAAAA TGGAGGTGAA CTGCTGAAAA ATCGCGTCGG GCGATTTAGT
    GAGAAGATTC GAGAAAGTGA TCCTCCACTC ATCGACTATC TAGCAGCCAA CGGCTTTGAC
    CTGCTCACCC ACAGCCACAC ACCTCCGGAG AAATCTGCTA CTGTTGCTTT GGATGAAGTC
    ATGTGTTTGA AGAGGCTATT GGTTGGGAGT ATTAGCCTGT CAGATGATGC TCAGACTCTT
    GGGGAAAACG GTGGGATGGC ACCTGAAAGT CAGGATGCAG ATGGAACTCA ACTGTCAGTG
    GAGGAATCGA GCACAGAAGC AGGTCCTGAA GTAGAAGAGC ATGAGGAAGA TGTAGAGAAT
    AAAGGAGAAG AGGAGAAAGG TATCAGTGCT CCTCTCGTGC TGTCTGAGGA GCGCATGGAG
    GAGGTGCAGA TGAGACTGCA GGCTCTGGAG GAGCAGCTGA AGAGATTGAG GGTGAGGAGC
    AAACTGGTGC ATTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017206841.1
    CDS373..3510
    Translation

    Target ORF information:

    RefSeq Version XM_017351352.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 1b (arhgef1b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351352.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGATTACG AAGACGCCCA TAATGGGCGC GGGCTGTCAG GTGCTCAGGG TTTAAATCCA 
    GTCATGAACA TAATCGGAGC AGAGGATGAA GACTTTGAGA ATGATGAGCA GCCTATGGTG
    GACGATCAGT GCATTCACTT TAACAGTATT GAGCTGTTGA AGTCCAGGCC CACCCACCTG
    CTGGTGTTCC TTCATCATGT CATGCTACAG TTTGACTGTG CTCCTGTGCT ATGCTACCTT
    CATGCTGACC TCATGAAGAA CCTCAATACC AAAGACACCA AGAAGCACTT TGGGGAATTC
    TACAACACTT TCCTGGAGAA AGGAGCGATT CTCAAAGTAT CTGTACCAAG TAACATCGCC
    CCTGAAATAG ATCGCTTGCG TCCAGAACTG ATCAATGAAG AGCAGCAAAA ACGTTACGCT
    AATGAAATAC AGTTTGCGCA GAGTCCAGAG ATACTGCGAC AGCTGGAAGA CTTCAGGCAA
    AAGAGGATGA TGGGAATGAC ACCTAATGAG CGGGAATTAA ATGATGTGGA ATCCCATCGA
    CCCACTGATC GCATCCCTAT GGACATGAAG GAGAAGGCTG TCGCTGAATC CCTGCTGGAA
    AAGATGGTTG AGGCCAACCC CTCAATTGTT CCTGACAAGG ACAAAAGTAA CTCCATCTTT
    GGAGCAGTGG CTTATTATAT GAAGTATCTT GGAGTCAAGA CCAGAGCTTT TGACAACAAG
    AAGTCCAAGT CAGGATGGCG GCCCTCGGTT CCATCTATGC TGAAGCCATG GTCAGTCAAC
    AAGCAGATTA AACCAGTCAA ACCGCCTGGA ATCGGCTATG ACGGTAAGCA AAGTAGATTG
    GACTTTGAAG CAAAGCAAAA CAAACAACCT CCTCATCGAG TGTCTGTTAG TGATAGTGTC
    ACCCCTCCAG GGCGAAAAAC TGGTATTACC GTGTCAGGGA GCACTCATGG GTCAGAGAGC
    AGTGAAGAGT TACCCATCAG TGTGAGTGTG ACTCCCAGCC CTGACTTTCA AACTGATACA
    GTAGGTATCA TCAACAATCG CACAGACCCC AAACCAGCCT CTGAGGGTGG AGAAGTGTCT
    CCAGTCAGCA CTGGTGGGAC TGTGTCCATC TGTGAACCTC TCGCTGTCAT TGATGTCCCT
    CCAGAAGACA TTCAGGACAG CGGCAGAAAG AAGCCAAGGA ATGTGCGTCG CAGTGAAAGC
    CTTTGTGTGG AGCGGCGTCA CTCTCGTCGT GGTGGCTCTA CTCGGGCCAA GCAGTCTCGT
    TCCCGCAGCG ACGTGGACCT CCAGTCAACT TCCACATCCC ATCCCCTCTC ACCCTCCCCA
    CAGCATTCAG TCTCCGTGGA AGGTGACAAA ACACCGGAAA TCGCTCTCCT CACTCCCACT
    TTTGTCCCGG CCCCTCAGCA AGAAGAGATA GAGCCCCGTC TCCTGGAGCT CGAGCAGGAC
    CCACTCAACT GGAGAGAACT TGCTTCCAGT GAAAGCCTGC ATAAGCTGAG CAAGAAGGAA
    GCCAAAAGAC AAGAAGTCAT AAATGAGCTG TTTGTGACAG AACAAGCACA CGTCCGTATG
    CTGAGTGTAT TGCAGACAGT GTTTTCTCGT CCACTTGAGA AGCAAGAGTA TATGACTGCA
    AATGAGGTGG CCACCATCTT CTCCAGCCTG GATGACATCA TAGAGATGCA CTATGTTTTC
    TATGAGAACC TGAAGAAACT GCGTGAAGAA GATGAATACA TTGTCAAAAC TATCAGCACA
    CCACTTCTCA ACAGATTCAG TGGATCTGAA GGAGAATGGT TTCAAAAGCT CACAGCACGC
    TTCTGTAGCT ACCAGTCCTG GGCTCTGGAG CAGATCAAGA GCAGGCAGAA GAAGGACCCC
    CGATTCAATG CTTTTATACT GGAAGCAGAG AGTAAGCCAC AGTGTCGGAG GCTACAGCTA
    AAGGATATCA TACCCATAGA GATGCAGAGA CTAACAAAAT ACCCTCTTCT GCTGGAAAAC
    ATTGCTAAGA ACACAGAGGA TGCAACAGAA AAAGAGAAAA TTAACCAAGC AGCAGAATCC
    TGCCGTAAGA TCCTCAACCA TGTCAATGAA GAAGTCAAAC AAATGGAGAA CCTGCTGATT
    TTGAAAGACT ACCAGCGTCG ACTGGATACC TCAGGACTGA AACCCAGCAA TGAACTCTAC
    AGTGAGTACA AGACCATTGA TCTGACCAAC CGAACGATGC TGTTTGAAGG ACCTCTGACA
    TGGAGGGTGA CCAAAGAGAA AGCAATCGAT GTGCACTGTG TGTTGCTGTC AGACCTGCTG
    GTCTTGTTTC AGAAGCAGGA TGATAAGATG CTTTTGAAGT GTCAGAGCAA AAGCAACATC
    GCCGTTCAGG AAGGCAAGCA GATGCTGAGT CCAATCATCA AGCTGGAGTC TGTTTTCCTC
    AGAGAGGTGG CCACTGACCC GAAGGCCCTG TATGTTATCT TCACCTGGGA CAGTGGAGCA
    CAGATCTATG AACTGGTGGC TCAGTCTGTC GGGGAGAGGA AAAACTGGAC AGAGGCGATT
    AAGTCTGCGG TTGATGAGCT GAAGAAGGCA TCAAAGAGCA ACACCAAACT GGAAAGGAAT
    AGCAGGGCTG GCAGTGTGCC AAACAACATT GCTGTACCTC TATATGGCCC AGCTCATCCT
    CCTCTTAGCC CCACTGAAAA TGGAGGTGAA CTGCTGAAAA ATCGCGTCGG GCGATTTAGT
    GAGAAGATTC GAGAAAGTGA TCCTCCACTC ATCGACTATC TAGCAGCCAA CGGCTTTGAC
    CTGCTCACCC ACAGCCACAC ACCTCCGGAG AAATCTGCTA CTGTTGCTTT GGATGAAGTC
    ATGTGTTTGA AGAGGCTATT GGTTGGGAGT ATTAGCCTGT CAGATGATGC TCAGACTCTT
    GGGGAAAACG GTGGGATGGC ACCTGAAAGT CAGGATGCAG ATGGAACTCA ACTGTCAGTG
    GAGGAATCGA GCACAGAAGC AGGTCCTGAA GTAGAAGAGC ATGAGGAAGA TGTAGAGAAT
    AAAGGAGAAG AGGAGAAAGG TATCAGTGCT CCTCTCGTGC TGTCTGAGGA GCGCATGGAG
    GAGGTGCAGA TGAGACTGCA GGCTCTGGAG GAGCAGCTGA AGAGATTGAG GGTGAGGAGC
    AAACTGGTGC ATTTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa42711
    Clone ID Related Accession (Same CDS sequence) NM_001044848.2
    Accession Version NM_001044848.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3018bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2018-11-23
    Organism Danio rerio(zebrafish)
    Product rho guanine nucleotide exchange factor 1
    Comment Comment: INFERRED REFSEQ: This record is predicted by genome sequence analysis and is not yet supported by experimental evidence. The reference sequence was derived from BX571757.8 and BX571738.12. On Jun 20, 2015 this sequence version replaced NM_001044848.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01001482.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGATTACG AAGACGCCCA TAATGGGCGC GGGCTGTCAG GTGCTCAGGG TTTAAATCCA 
    GTCATGAACA TAATCGGAGC AGAGGATGAA GACTTTGAGA ATGATGAGCA GCCTATGGTG
    GACGATCAGT GCATTCACTT TAACAGTATT GAGCTGTTGA AGTCCAGGCC CACCCACCTG
    CTGGTGTTCC TTCATCATGT CATGCTACAG TTTGACTGTG CTCCTGTGCT ATGCTACCTT
    CATGCTGACC TCATGAAGAA CCTCAATACC AAAGACACCA AGAAGCACTT TGGGGAATTC
    TACAACACTT TCCTGGAGAA AGGAGCGATT CTCAAAGTAT CTGTACCAAG TAACATCGCC
    CCTGAAATAG ATCGCTTGCG TCCAGAACTG ATCAATGAAG AGCAGCAAAA ACGTTACGCT
    AATGAAATAC AGTTTGCGCA GAGTCCAGAG ATACTGCGAC AGCTGGAAGA CTTCAGGCAA
    AAGAGGATGA TGGGAATGAC ACCTAATGAG CGGGAATTAA ATGATGTGGA ATCCCATCGA
    CCCACTGATC GCATCCCTAT GGACATGAAG GAGAAGGCTG TCGCTGAATC CCTGCTGGAA
    AAGATGGTTG AGGCCAACCC CTCAATTGTT CCTGACAAGG ACAAAAGTAA CTCCATCTTT
    GGAGCAGTGG CTTATTATAT GAAGTATCTT GGAGTCAAGA CCAGAGCTTT TGACAACAAG
    AAGTCCAAGT CAGGATGGCG GCCCTCGGTT CCATCTATGC TGAAGCCATG GTCAGTCAAC
    AAGCAGATTA AACCAGTCAA ACCGCCTGGA ATCGGCTATG ACGGTAAGCA AAGTAGATTG
    GACTTTGAAG CAAAGCAAAA CAAACAACCT CCTCATCGAG TGTCTGTTAG TGATAGTGTC
    ACCCCTCCAG GGCGAAAAAC TGGTATTACC GTGTCAGGGA GCACTCATGG GTCAGAGAGC
    AGTGAAGAGT TACCCATCAG TGTGAGTGTG ACTCCCAGCC CTGACTTTCA AACTGATACA
    GTAGGTATCA TCAACAATCG CACAGACCCC AAACCAGCCT CTGAGGGTGG AGAAGTGTCT
    CCAGTCAGCA CTGGTGGGAC TGTGTCCATC TGTGAACCTC TCGCTGTCAT TGATGTCCCT
    CCAGAAGACA TTCAGGACAG CGGCAGCGTG GAAGGTGACA AAACACCGGA AATCGCTCTC
    CTCACTCCCA CTTTTGTCCC GGCCCCTCAG CAAGAAGAGA TAGAGCCCCG TCTCCTGGAG
    CTCGAGCAGG ACCCACTCAA CTGGAGAGAA CTTGCTTCCA GTGAAAGCCT GCATAAGCTG
    AGCAAGAAGG AAGCCAAAAG ACAAGAAGTC ATAAATGAGC TGTTTGTGAC AGAACAAGCA
    CACGTCCGTA TGCTGAGTGT ATTGCAGACA GTGTTTTCTC GTCCACTTGA GAAGCAAGAG
    TATATGACTG CAAATGAGGT GGCCACCATC TTCTCCAGCC TGGATGACAT CATAGAGATG
    CACTATGTTT TCTATGAGAA CCTGAAGAAA CTGCGTGAAG AAGATGAATA CATTGTCAAA
    ACTATCAGCA CACCACTTCT CAACAGATTC AGTGGATCTG AAGGAGAATG GTTTCAAAAG
    CTCACAGCAC GCTTCTGTAG CTACCAGTCC TGGGCTCTGG AGCAGATCAA GAGCAGGCAG
    AAGAAGGACC CCCGATTCAA TGCTTTTATA CTGGAAGCAG AGAGTAAGCC ACAGTGTCGG
    AGGCTACAGC TAAAGGATAT CATACCCATA GAGATGCAGA GACTAACAAA ATACCCTCTT
    CTGCTGGAAA ACATTGCTAA GAACACAGAG GATGCAACAG AAAAAGAGAA AATTAACCAA
    GCAGCAGAAT CCTGCCGTAA GATCCTCAAC CATGTCAATG AAGAAGTCAA ACAAATGGAG
    AACCTGCTGA TTTTGAAAGA CTACCAGCGT CGACTGGATA CCTCAGGACT GAAACCCAGC
    AATGAACTCT ACAGTGAGTA CAAGACCATT GATCTGACCA ACCGAACGAT GCTGTTTGAA
    GGACCTCTGA CATGGAGGGT GACCAAAGAG AAAGCAATCG ATGTGCACTG TGTGTTGCTG
    TCAGACCTGC TGGTCTTGTT TCAGAAGCAG GATGATAAGA TGCTTTTGAA GTGTCAGAGC
    AAAAGCAACA TCGCCGTTCA GGAAGGCAAG CAGATGCTGA GTCCAATCAT CAAGCTGGAG
    TCTGTTTTCC TCAGAGAGGT GGCCACTGAC CCGAAGGCCC TGTATGTTAT CTTCACCTGG
    GACAGTGGAG CACAGATCTA TGAACTGGTG GCTCAGTCTG TCGGGGAGAG GAAAAACTGG
    ACAGAGGCGA TTAAGTCTGC GGTTGATGAG CTGAAGAAGG CATCAAAGAG CAACACCAAA
    CTGGAAAGGA ATAGCAGGGC TGGCAGTGTG CCAAACAACA TTGCTGTACC TCTATATGGC
    CCAGCTCATC CTCCTCTTAG CCCCACTGAA AATGGAGGTG AACTGCTGAA AAATCGCGTC
    GGGCGATTTA GTGAGAAGAT TCGAGAAAGT GATCCTCCAC TCATCGACTA TCTAGCAGCC
    AACGGCTTTG ACCTGCTCAC CCACAGCCAC ACACCTCCGG AGAAATCTGC TACTGTTGCT
    TTGGATGAAG TCATGTGTTT GAAGAGGCTA TTGGTTGGGA GTATTAGCCT GTCAGATGAT
    GCTCAGACTC TTGGGGAAAA CGGTGGGATG GCACCTGAAA GTCAGGATGC AGATGGAACT
    CAACTGTCAG TGGAGGAATC GAGCACAGAA GCAGGTCCTG AAGTAGAAGA GCATGAGGAA
    GATGTAGAGA ATAAAGGAGA AGAGGAGAAA GGTATCAGTG CTCCTCTCGT GCTGTCTGAG
    GAGCGCATGG AGGAGGTGCA GATGAGACTG CAGGCTCTGG AGGAGCAGCT GAAGAGATTG
    AGGTTGGTTG AGGAGGACCA TTATAAATTA CAAGAAGCTC TTGCTAAATA CACACTTCAG
    GGGGGCCATT ACAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001038313.2
    CDS96..3113
    Translation

    Target ORF information:

    RefSeq Version NM_001044848.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio Rho guanine nucleotide exchange factor (GEF) 1b (arhgef1b), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001044848.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    ATGGATTACG AAGACGCCCA TAATGGGCGC GGGCTGTCAG GTGCTCAGGG TTTAAATCCA 
    GTCATGAACA TAATCGGAGC AGAGGATGAA GACTTTGAGA ATGATGAGCA GCCTATGGTG
    GACGATCAGT GCATTCACTT TAACAGTATT GAGCTGTTGA AGTCCAGGCC CACCCACCTG
    CTGGTGTTCC TTCATCATGT CATGCTACAG TTTGACTGTG CTCCTGTGCT ATGCTACCTT
    CATGCTGACC TCATGAAGAA CCTCAATACC AAAGACACCA AGAAGCACTT TGGGGAATTC
    TACAACACTT TCCTGGAGAA AGGAGCGATT CTCAAAGTAT CTGTACCAAG TAACATCGCC
    CCTGAAATAG ATCGCTTGCG TCCAGAACTG ATCAATGAAG AGCAGCAAAA ACGTTACGCT
    AATGAAATAC AGTTTGCGCA GAGTCCAGAG ATACTGCGAC AGCTGGAAGA CTTCAGGCAA
    AAGAGGATGA TGGGAATGAC ACCTAATGAG CGGGAATTAA ATGATGTGGA ATCCCATCGA
    CCCACTGATC GCATCCCTAT GGACATGAAG GAGAAGGCTG TCGCTGAATC CCTGCTGGAA
    AAGATGGTTG AGGCCAACCC CTCAATTGTT CCTGACAAGG ACAAAAGTAA CTCCATCTTT
    GGAGCAGTGG CTTATTATAT GAAGTATCTT GGAGTCAAGA CCAGAGCTTT TGACAACAAG
    AAGTCCAAGT CAGGATGGCG GCCCTCGGTT CCATCTATGC TGAAGCCATG GTCAGTCAAC
    AAGCAGATTA AACCAGTCAA ACCGCCTGGA ATCGGCTATG ACGGTAAGCA AAGTAGATTG
    GACTTTGAAG CAAAGCAAAA CAAACAACCT CCTCATCGAG TGTCTGTTAG TGATAGTGTC
    ACCCCTCCAG GGCGAAAAAC TGGTATTACC GTGTCAGGGA GCACTCATGG GTCAGAGAGC
    AGTGAAGAGT TACCCATCAG TGTGAGTGTG ACTCCCAGCC CTGACTTTCA AACTGATACA
    GTAGGTATCA TCAACAATCG CACAGACCCC AAACCAGCCT CTGAGGGTGG AGAAGTGTCT
    CCAGTCAGCA CTGGTGGGAC TGTGTCCATC TGTGAACCTC TCGCTGTCAT TGATGTCCCT
    CCAGAAGACA TTCAGGACAG CGGCAGCGTG GAAGGTGACA AAACACCGGA AATCGCTCTC
    CTCACTCCCA CTTTTGTCCC GGCCCCTCAG CAAGAAGAGA TAGAGCCCCG TCTCCTGGAG
    CTCGAGCAGG ACCCACTCAA CTGGAGAGAA CTTGCTTCCA GTGAAAGCCT GCATAAGCTG
    AGCAAGAAGG AAGCCAAAAG ACAAGAAGTC ATAAATGAGC TGTTTGTGAC AGAACAAGCA
    CACGTCCGTA TGCTGAGTGT ATTGCAGACA GTGTTTTCTC GTCCACTTGA GAAGCAAGAG
    TATATGACTG CAAATGAGGT GGCCACCATC TTCTCCAGCC TGGATGACAT CATAGAGATG
    CACTATGTTT TCTATGAGAA CCTGAAGAAA CTGCGTGAAG AAGATGAATA CATTGTCAAA
    ACTATCAGCA CACCACTTCT CAACAGATTC AGTGGATCTG AAGGAGAATG GTTTCAAAAG
    CTCACAGCAC GCTTCTGTAG CTACCAGTCC TGGGCTCTGG AGCAGATCAA GAGCAGGCAG
    AAGAAGGACC CCCGATTCAA TGCTTTTATA CTGGAAGCAG AGAGTAAGCC ACAGTGTCGG
    AGGCTACAGC TAAAGGATAT CATACCCATA GAGATGCAGA GACTAACAAA ATACCCTCTT
    CTGCTGGAAA ACATTGCTAA GAACACAGAG GATGCAACAG AAAAAGAGAA AATTAACCAA
    GCAGCAGAAT CCTGCCGTAA GATCCTCAAC CATGTCAATG AAGAAGTCAA ACAAATGGAG
    AACCTGCTGA TTTTGAAAGA CTACCAGCGT CGACTGGATA CCTCAGGACT GAAACCCAGC
    AATGAACTCT ACAGTGAGTA CAAGACCATT GATCTGACCA ACCGAACGAT GCTGTTTGAA
    GGACCTCTGA CATGGAGGGT GACCAAAGAG AAAGCAATCG ATGTGCACTG TGTGTTGCTG
    TCAGACCTGC TGGTCTTGTT TCAGAAGCAG GATGATAAGA TGCTTTTGAA GTGTCAGAGC
    AAAAGCAACA TCGCCGTTCA GGAAGGCAAG CAGATGCTGA GTCCAATCAT CAAGCTGGAG
    TCTGTTTTCC TCAGAGAGGT GGCCACTGAC CCGAAGGCCC TGTATGTTAT CTTCACCTGG
    GACAGTGGAG CACAGATCTA TGAACTGGTG GCTCAGTCTG TCGGGGAGAG GAAAAACTGG
    ACAGAGGCGA TTAAGTCTGC GGTTGATGAG CTGAAGAAGG CATCAAAGAG CAACACCAAA
    CTGGAAAGGA ATAGCAGGGC TGGCAGTGTG CCAAACAACA TTGCTGTACC TCTATATGGC
    CCAGCTCATC CTCCTCTTAG CCCCACTGAA AATGGAGGTG AACTGCTGAA AAATCGCGTC
    GGGCGATTTA GTGAGAAGAT TCGAGAAAGT GATCCTCCAC TCATCGACTA TCTAGCAGCC
    AACGGCTTTG ACCTGCTCAC CCACAGCCAC ACACCTCCGG AGAAATCTGC TACTGTTGCT
    TTGGATGAAG TCATGTGTTT GAAGAGGCTA TTGGTTGGGA GTATTAGCCT GTCAGATGAT
    GCTCAGACTC TTGGGGAAAA CGGTGGGATG GCACCTGAAA GTCAGGATGC AGATGGAACT
    CAACTGTCAG TGGAGGAATC GAGCACAGAA GCAGGTCCTG AAGTAGAAGA GCATGAGGAA
    GATGTAGAGA ATAAAGGAGA AGAGGAGAAA GGTATCAGTG CTCCTCTCGT GCTGTCTGAG
    GAGCGCATGG AGGAGGTGCA GATGAGACTG CAGGCTCTGG AGGAGCAGCT GAAGAGATTG
    AGGTTGGTTG AGGAGGACCA TTATAAATTA CAAGAAGCTC TTGCTAAATA CACACTTCAG
    GGGGGCCATT ACAATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.