selenoi cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	selenoi
Entrez Gene ID	557632
Full Name	selenoprotein I
Synonyms	ept1,seli,si:ch211-197l9.4
General protein information	Preferred Names selenoprotein I Names ethanolaminephosphotransferase 1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)
Summary	The multi-pass transmembrane protein encoded by this gene belongs to the CDP-alcohol phosphatidyltransferase class-I family. It catalyzes the transfer of phosphoethanolamine from CDP-ethanolamine to diacylglycerol to produce phosphatidylethanolamine, which is involved in the formation and maintenance of vesicular membranes, regulation of lipid metabolism, and protein folding. This protein is a selenoprotein, containing the rare selenocysteine (Sec) amino acid at its active site. Sec is encoded by the UGA codon, which normally signals translation termination. The 3' UTRs of selenoprotein mRNAs contain a conserved stem-loop structure, designated the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. [provided by RefSeq, Jul 2016].

mRNA and Protein(s)

mRNA	Protein	Name
NM_001329437.1	NP_001316366.1	ethanolaminephosphotransferase 1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Selenoprotein Gene Nomenclature.
Gladyshev VN, Arn?r ES, Berry MJ, Brigelius-Floh? R, Bruford EA, Burk RF, Carlson BA, Castellano S, Chavatte L, Conrad M, Copeland PR, Diamond AM, Driscoll DM, Ferreiro A, Floh? L, Green FR, Guig? R, Handy DE, Hatfield DL, Hesketh J, Hoffmann PR, Holmgren A, Hondal RJ, Howard MT, Huang K, Kim HY, Kim IY, K?hrle J, Krol A, Kryukov GV, Lee BJ, Lee BC, Lei XG, Liu Q, Lescure A, Lobanov AV, Loscalzo J, Maiorino M, Mariotti M, Sandeep Prabhu K, Rayman MP, Rozovsky S, Salinas G, Schmidt EE, Schomburg L, Schweizer U, Simonovi? M, Sunde RA, Tsuji PA, Tweedie S, Ursini F, Whanger PD, Zhang Y
The Journal of biological chemistry291(46)24036-24040(2016 Nov)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Composition and evolution of the vertebrate and mammalian selenoproteomes.
Mariotti M, Ridge PG, Zhang Y, Lobanov AV, Pringle TH, Guigo R, Hatfield DL, Gladyshev VN
PloS one7(3)e33066(2012)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following selenoi gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the selenoi cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa98463	NM_001329437.1 Latest version!	Danio rerio selenoprotein I (selenoi), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$223.30 $319.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

selenoi ( NM_001329437.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001316366.1 Danio rerio selenoprotein I (selenoi), mRNA.

Price & Availability↑

CloneID	ODa98463
Clone ID Related Accession (Same CDS sequence)	NM_001329437.1
Accession Version	NM_001329437.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1203bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	ethanolaminephosphotransferase 1
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from BX649257.8. On Jul 11, 2016 this sequence version replaced XM_009297003.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GFIL01035796.1, GDQH01003819.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGCTCTCT ACCACTATGT CACGCAAGAA CAACTCTCAG GTTTTGATAA ATACAAGTAC 
AGCGCTGTGG ATTCAAACCC CCTTTCTATC TACGTCATGC ACCCTTTCTG GAACTCAGTG 
GTGAAGGTCT TACCCACATG GCTTGCCCCA AACCTCATCA CATTTACAGG GTTCATGTTC 
CTGGTACTCA CCTTCACCAT GCTATCCTTC TTCGACTTTG ACTTTTATGC CTCAGGTGAA 
GGCCACACGC ATGTGCCAAG TTGGGTGTGG ATTGCTGCTG GTTTGTTCAA CTTTCTAGCT 
TATACCTTAG ATGGTGTGGA TGGAAAACAG GCACGGAGAA CCAACTCCAG CACTCCTCTG 
GGTGAACTGT TTGATCACGG TCTGGACAGC TGGGCCTGTG TATTTTTTGT TGCCACAGTG 
TACTCTGTCT TTGGACGGGG TGAGACGGGG GTCAGCGTGG TGACACTCTA TTATCTGTTA 
TGGGTCGTCC TGTTTTCCTT CATCCTGTCT CACTGGGAGA AGTACAACAC TGGAATTTTG 
TTTCTGCCCT GGGGCTACGA CATCAGTCAA GTGACTATCT CCATCGTCTA CATTATAACA 
GCAGTAGTTG GAGTGGAAAC GTGGTACAAA CCTGTGTTTC TGAACATTCA CTACAGAGAT 
CTCTTCACTG TTATGATTAT CGGTTGTTTA TTTGTTGTAA CTCTGCCAAT GAGTCTCTAC 
AATGTCTTCA AGGCGTATCG TAACAACACC TTGAAGCACA GTTCACTGTA TGAGGCCTTG 
TTGCCGTTTT TTTCTCCTGT GCTTTTATTC GTGCTGTCTA CACTGTGGAT CAGTTTCTCT 
CCAACAAACA TCGTCCAGAA GCAGCCCAGG ATCTTTTATC TCATGGTGGG AACGGCCTTC 
TCCAACGTTA CATGCAAGCT CATTGTGTGC CAGATGAGTA ATACTCGATG TAAGCCACTG 
AGCTTGTTGT TGTTGCCGAT GAGCATCGTG GTGCTGCTAG TGATTTCTGG GACTGTGCAG 
GGAGGAGAAT CGCTAGTCTT GTTTATATGG ACTGCTGTAG TCCTGCTCAC TCACATACAC 
TACGGAGTCT CGGTTGTGAA GCAGTTGAGC GATCATTTTA ACATATTCGC TTTCTCACTA 
AAAAAGCCCA ATTCGGACTG ACAGGACGAG GAGAAAATCG GCCTGAAGGC TGCTGAGGTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001316366.1
CDS	119..1321
Translation	MALYHYVTQEQLSGFDKYKYSAVDSNPLSIYVMHPFWNSVVKVLPTWLAPNLITFTGFMFLVLTFTMLSFFDFDFYASGEGHTHVPSWVWIAAGLFNFLAYTLDGVDGKQARRTNSSTPLGELFDHGLDSWACVFFVATVYSVFGRGETGVSVVTLYYLLWVVLFSFILSHWEKYNTGILFLPWGYDISQVTISIVYIITAVVGVETWYKPVFLNIHYRDLFTVMIIGCLFVVTLPMSLYNVFKAYRNNTLKHSSLYEALLPFFSPVLLFVLSTLWISFSPTNIVQKQPRIFYLMVGTAFSNVTCKLIVCQMSNTRCKPLSLLLLPMSIVVLLVISGTVQGGESLVLFIWTAVVLLTHIHYGVSVVKQLSDHFNIFAFSLKKPNSD*QDEEKIGLKAAEV

Target ORF information:

RefSeq Version	NM_001329437.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio selenoprotein I (selenoi), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001329437.1

ORF Insert Sequence:

ATGGCTCTCT ACCACTATGT CACGCAAGAA CAACTCTCAG GTTTTGATAA ATACAAGTAC 
AGCGCTGTGG ATTCAAACCC CCTTTCTATC TACGTCATGC ACCCTTTCTG GAACTCAGTG 
GTGAAGGTCT TACCCACATG GCTTGCCCCA AACCTCATCA CATTTACAGG GTTCATGTTC 
CTGGTACTCA CCTTCACCAT GCTATCCTTC TTCGACTTTG ACTTTTATGC CTCAGGTGAA 
GGCCACACGC ATGTGCCAAG TTGGGTGTGG ATTGCTGCTG GTTTGTTCAA CTTTCTAGCT 
TATACCTTAG ATGGTGTGGA TGGAAAACAG GCACGGAGAA CCAACTCCAG CACTCCTCTG 
GGTGAACTGT TTGATCACGG TCTGGACAGC TGGGCCTGTG TATTTTTTGT TGCCACAGTG 
TACTCTGTCT TTGGACGGGG TGAGACGGGG GTCAGCGTGG TGACACTCTA TTATCTGTTA 
TGGGTCGTCC TGTTTTCCTT CATCCTGTCT CACTGGGAGA AGTACAACAC TGGAATTTTG 
TTTCTGCCCT GGGGCTACGA CATCAGTCAA GTGACTATCT CCATCGTCTA CATTATAACA 
GCAGTAGTTG GAGTGGAAAC GTGGTACAAA CCTGTGTTTC TGAACATTCA CTACAGAGAT 
CTCTTCACTG TTATGATTAT CGGTTGTTTA TTTGTTGTAA CTCTGCCAAT GAGTCTCTAC 
AATGTCTTCA AGGCGTATCG TAACAACACC TTGAAGCACA GTTCACTGTA TGAGGCCTTG 
TTGCCGTTTT TTTCTCCTGT GCTTTTATTC GTGCTGTCTA CACTGTGGAT CAGTTTCTCT 
CCAACAAACA TCGTCCAGAA GCAGCCCAGG ATCTTTTATC TCATGGTGGG AACGGCCTTC 
TCCAACGTTA CATGCAAGCT CATTGTGTGC CAGATGAGTA ATACTCGATG TAAGCCACTG 
AGCTTGTTGT TGTTGCCGAT GAGCATCGTG GTGCTGCTAG TGATTTCTGG GACTGTGCAG 
GGAGGAGAAT CGCTAGTCTT GTTTATATGG ACTGCTGTAG TCCTGCTCAC TCACATACAC 
TACGGAGTCT CGGTTGTGAA GCAGTTGAGC GATCATTTTA ACATATTCGC TTTCTCACTA 
AAAAAGCCCA ATTCGGACTG ACAGGACGAG GAGAAAATCG GCCTGAAGGC TGCTGAGGTC 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Selenoprotein Gene Nomenclature.
The Journal of biological chemistry291(46)24036-24040(2016 Nov)
Gladyshev VN,Arn?r ES,Berry MJ,Brigelius-Floh? R,Bruford EA,Burk RF,Carlson BA,Castellano S,Chavatte L,Conrad M,Copeland PR,Diamond AM,Driscoll DM,Ferreiro A,Floh? L,Green FR,Guig? R,Handy DE,Hatfield DL,Hesketh J,Hoffmann PR,Holmgren A,Hondal RJ,Howard MT,Huang K,Kim HY,Kim IY,K?hrle J,Krol A,Kryukov GV,Lee BJ,Lee BC,Lei XG,Liu Q,Lescure A,Lobanov AV,Loscalzo J,Maiorino M,Mariotti M,Sandeep Prabhu K,Rayman MP,Rozovsky S,Salinas G,Schmidt EE,Schomburg L,Schweizer U,Simonovi? M,Sunde RA,Tsuji PA,Tweedie S,Ursini F,Whanger PD,Zhang Y

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Composition and evolution of the vertebrate and mammalian selenoproteomes.
PloS one7(3)e33066(2012)
Mariotti M,Ridge PG,Zhang Y,Lobanov AV,Pringle TH,Guigo R,Hatfield DL,Gladyshev VN

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selenoi cDNA ORF clone, Danio rerio(zebrafish)

Gene

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Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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