DAAM1A cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	DAAM1A
Entrez Gene ID	557451
Full Name	dishevelled associated activator of morphogenesis 1a
Synonyms	daam1
General protein information	Preferred Names dishevelled associated activator of morphogenesis 1a Names disheveled-associated activator of morphogenesis 1 dishevelled associated activator of morphogenesis 1
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_702261.8	XP_707353.4	disheveled-associated activator of morphogenesis 1 isoform X1
XM_017351772.1	XP_017207261.1	disheveled-associated activator of morphogenesis 1 isoform X2
XM_702261.9	XP_707353.4	disheveled-associated activator of morphogenesis 1 isoform X1
XM_017351772.2	XP_017207261.1	disheveled-associated activator of morphogenesis 1 isoform X2
XM_017351771.2	XP_017207260.1	disheveled-associated activator of morphogenesis 1 isoform X1
XM_017351771.1	XP_017207260.1	disheveled-associated activator of morphogenesis 1 isoform X1

Pathways from BioSystems
Gene Ontology

The following DAAM1A gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the DAAM1A cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa22320	XM_017351771.2 Latest version!	Danio rerio dishevelled associated activator of morphogenesis 1a (daam1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa22320	XM_702261.9 Latest version!	Danio rerio dishevelled associated activator of morphogenesis 1a (daam1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa22321	XM_017351772.2 Latest version!	Danio rerio dishevelled associated activator of morphogenesis 1a (daam1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
ODa22320	XM_702261.8	Danio rerio dishevelled associated activator of morphogenesis 1a (daam1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa22321	XM_017351772.1	Danio rerio dishevelled associated activator of morphogenesis 1a (daam1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa22320	XM_017351771.1	Danio rerio dishevelled associated activator of morphogenesis 1a (daam1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

DAAM1A ( XM_702261.8 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_707353.4 Danio rerio dishevelled associated activator of morphogenesis 1a (daam1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa22320
Clone ID Related Accession (Same CDS sequence)	XM_702261.8 , XM_702261.9 , XM_017351771.2 , XM_017351771.1
Accession Version	XM_702261.8	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	disheveled-associated activator of morphogenesis 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_702261.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCC CACGTAAGCG AGGAGGTCGG GGCCTGTCAT TCCTCTGCTG CTGTCTAAAA 
GGGAGCGATC ATCCCGAAAT CACCTACAGC CTCCGTCATG GCAGCAACTT TACACTGCAG 
ACCATGGAGC CCACCCTGCC AATGCCCCCC ACTGAGGAGC TGGATGCTAT GTTCACTGAA 
CTGGTGGATG AGCTGGATCT CACAGAGAAA CATAGAGCGG CCATGTTTGC CCTGCCTGCC 
GAGAAGAAAT GGCAAATCTA CTGTAGCAAG AAAAAGGAAC AGGAAGAGAA TAAAGGGGCG 
ACCAGCTGGC CTGAATTCTA CATCGATCAG ATCAATTCTA TGGCTGCTAG GAAGTCTTTG 
CTAGCTCTGG AGAAGGAGGA TGAGGAGGAG AGGAATAAAA CCATAGAGAG CCTGAAAACT 
GCTCTCCGCA CACAGCCCAT GAGGTTCGTG ACGCGCTTCA TAGACCAGGA TGGCCTGACG 
TGCATCTTAA ATTTTCTGAA AACGATGGAC TATGAGACGA CCGAATCGCA GATCCACACG 
TCGCTCATCG GCTGCATTAA AGCCCTGATG AACAACTCTC AGGGCCGAGC GCACGTGCTC 
TCGCATTCAG AAAGCATCAA CATCATTGCT CAAAGCCTGG CCACCGAGAA CATCAAGACT 
AAAGTGGCTG TTCTGGAGAT CATGGGTGCT GTTTGTTTGG TTCCTGGAGG ACACAAGAAA 
ATTCTGGAAG CCATGCTGCA TTATCAGAAG TTTGCCTGCG AGCGGACGCG CTTTCAGACT 
CTCCTGAATG ACTTGGACAG GAGTACGGGC CGCTACAGAG ATGAGGTGAA CCTCAAGACG 
GCCATCATGT CCTTCATCAA CGCCGTCCTC AGTCAAGGAG CAGGGGAGAC CAGTTTGGAG 
TTCAGAATTC ATTTGCGGTA TGAGTTTCTA ATGCTGGGCA TCCAACCAGT GATTGATAAA 
TTACGGTCCC ATGAAAACTC CACGTTAGAT CGGCATTTGG ACTTCTTTGA GATGCTACGA 
AATGAAGATG AATTAGCTCT CGCTAAGCGG TTTGAAAACG TTCATGTAGA TACTAAAAGT 
GCCACTCAGA TGTTCGAGCT CATCCGGAAG AGGATAAACC ACACAGACGC CTTCCCGCAC 
TTTATATCCG TCTTACAGCA CTGTCTTCAT ATGCCTTACA AGAAAACTGG GAACACTGTG 
CAGTACTGGG TTCTCCTAGA CAGAATTGTC CAGCAGATCA TCCTGCAGAA CGACAAGGGG 
CTCGACCCAG ATGTGGCACC GCTGGAGAAC TTTGATGTCA AAAACGTGGT GCGAATGCTG 
GTCAATGAGA ACGAGGTGAA ACAATGGAAA GAGCAAGCGG AAAAAATGAG GAAAGAACAT 
AATGAACTTC AGCAGAAGCT GGAAAAGAAA GAAAGGGAGT GTGATGCTAA AGCCCAAGAG 
AAGGAAGAAA TGATGCAAAC GCTCAATAAG ATGAAAGAGA AGCTGGAAAA GGAAAGCAGT 
GAACATAAGC TTGTTAAGCA GCAAGTGGCT GACCTTTCTG CCCGGCTACA TGAGATGAGT 
AATAGGACAA ATATCCCTGG TGGCCCTCCC CTCGCCCCTC TGGGTCCTGG TCTTCCACCT 
CCTCCTCCAC CTCCGGGTCA GGCTGGTCTT CCTCCTCCAC CTCCTCCAGG AGGAGCACCA 
CCACCACCTC CGCCTCCCCC CGGTGGACCT CCTCCTCCTC CAGGCCTGCT TGGCTTCGGT 
GCTCCTCCTC CTCCTGGAGG TCTCTTGGGC TCCACACTGA AGAAGAAGAA CATCCCGCAA 
CCATCCAACC CGCTCAAGTC TTTTAACTGG ACCAAACTCA GTGAAAATAA GCTGGAAGGG 
ACAGTGTGGC TGGATCTGGA CGACGTCAGA GTCTTCAAAC AATTAGATCT GGAGGATATT 
GAGAAAACAT TCTCTGCTTA TCAGCGACAG CAGGACTTTC TGTTCAATAA CTTTAGACAG 
AAAGAGTCCG AGGACGACAC TGTCACTTCT AAAAAGGTCA AAGAACTGTC TGTCATCGAT 
GGACGACGTG CGCAAAACTG CAACATCCTT CTCTCCAAGT TGAAACTGTC CAACGAGGAG 
ATCAAGAGAG CCATATTGAC CATGGATGAG CAGGAGGATC TGCCCAAAGA CATGCTGGAG 
CAGCTGTTGA AGTTTGTGCC AGAAAAAAGT GATGTAGACC TTCTGGAGGA GCACAAACAT 
GAGCTGGAGC GAATGGCGAA GGCAGACCGC TTCCTCTACG AGATGAGCAG AATCAACCAC 
TACCAGCAAA GACTGCAGTC TCTTTACTTT AAAAAGAAGT TTGCAGAAAG AATAGCAGAA 
ATCAAGCCCA AAGTGGAAGC TCTCAGTAAA GCATCTAAAG AGGTTCTTCA GAGCAAGAAT 
CTCCGTCAGC TTCTGGAGAT TGTCCTGGCC TTCGGAAACT ACATGAACAA AGGCCAGAGA 
GGCAATGCTT ACGGCTTCAA AGTGTCCTCT CTCAACAAGA TCGCTGACAC CAAATCCAGC 
ATCGACAAAA ACGTCACTCT TCTGCATTAC CTGATTACCG TTCTTGAGCA GAAATACCCC 
AAAGTCAGTC TGATTCACGA GGATCTGCAG AACGTTCCAG TTGCGGCCAA AGTCAACATG 
ACCGAACTGG AGAAAGACAT TAACAATCTG CGCTCTGGCT TGAAAAGCGT GGAGACGGAG 
CTGGAGTACC AGAGGACTCA ACCTCAGACC TATGGAGACA AGTTTGTGTC TGTAGTGAGT 
CAGTTCATCA CTGTGGCTGG ATTCAGTTTC TCTGACATTG AGGACTCTCT TCAGGATGCT 
AAGGATTCGT TTGGGAAGGC CGTGCAGCAC TTCGGTGAGG ACGCCACACG CATGCAGCCT 
GATGAGTTCT TCGGCATCTT CGATCAGTTC CTGCAGGGCT TCAGTGAAGC TAAACAAGAC 
AATGAGAACA TGAGACGACG GAAAGAGGAG GAGGAGCGCA GGGCCCGTAT GGAGGCACAG 
CTCAAGGAGC AGCGCGAGAG GGAAAGAAAA TCCCGGAAGG CTAAAGAAAA CTGCGAGGAG 
GACGGAGAGT TCGACGACCT GGTGTCTGCA CTGCGATCTG GGGAGGTGTT TGACAAAGAT 
ATGTCAAAGA TGAAACACAA CCGTAAACGT CCCGTCAAAT CGAGTGCAGA AAGCAGCAGA 
GAAAGACCCG TCACCAAACT CAACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_707353.4
CDS	411..3620
Translation	MMAPRKRGGRGLSFLCCCLKGSDHPEITYSLRHGSNFTLQTMEPTLPMPPTEELDAMFTELVDELDLTEKHRAAMFALPAEKKWQIYCSKKKEQEENKGATSWPEFYIDQINSMAARKSLLALEKEDEEERNKTIESLKTALRTQPMRFVTRFIDQDGLTCILNFLKTMDYETTESQIHTSLIGCIKALMNNSQGRAHVLSHSESINIIAQSLATENIKTKVAVLEIMGAVCLVPGGHKKILEAMLHYQKFACERTRFQTLLNDLDRSTGRYRDEVNLKTAIMSFINAVLSQGAGETSLEFRIHLRYEFLMLGIQPVIDKLRSHENSTLDRHLDFFEMLRNEDELALAKRFENVHVDTKSATQMFELIRKRINHTDAFPHFISVLQHCLHMPYKKTGNTVQYWVLLDRIVQQIILQNDKGLDPDVAPLENFDVKNVVRMLVNENEVKQWKEQAEKMRKEHNELQQKLEKKERECDAKAQEKEEMMQTLNKMKEKLEKESSEHKLVKQQVADLSARLHEMSNRTNIPGGPPLAPLGPGLPPPPPPPGQAGLPPPPPPGGAPPPPPPPPGGPPPPPGLLGFGAPPPPGGLLGSTLKKKNIPQPSNPLKSFNWTKLSENKLEGTVWLDLDDVRVFKQLDLEDIEKTFSAYQRQQDFLFNNFRQKESEDDTVTSKKVKELSVIDGRRAQNCNILLSKLKLSNEEIKRAILTMDEQEDLPKDMLEQLLKFVPEKSDVDLLEEHKHELERMAKADRFLYEMSRINHYQQRLQSLYFKKKFAERIAEIKPKVEALSKASKEVLQSKNLRQLLEIVLAFGNYMNKGQRGNAYGFKVSSLNKIADTKSSIDKNVTLLHYLITVLEQKYPKVSLIHEDLQNVPVAAKVNMTELEKDINNLRSGLKSVETELEYQRTQPQTYGDKFVSVVSQFITVAGFSFSDIEDSLQDAKDSFGKAVQHFGEDATRMQPDEFFGIFDQFLQGFSEAKQDNENMRRRKEEEERRARMEAQLKEQRERERKSRKAKENCEEDGEFDDLVSALRSGEVFDKDMSKMKHNRKRPVKSSAESSRERPVTKLNF

Target ORF information:

RefSeq Version	XM_702261.8
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio dishevelled associated activator of morphogenesis 1a (daam1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_702261.8

ORF Insert Sequence:

ATGATGGCCC CACGTAAGCG AGGAGGTCGG GGCCTGTCAT TCCTCTGCTG CTGTCTAAAA 
GGGAGCGATC ATCCCGAAAT CACCTACAGC CTCCGTCATG GCAGCAACTT TACACTGCAG 
ACCATGGAGC CCACCCTGCC AATGCCCCCC ACTGAGGAGC TGGATGCTAT GTTCACTGAA 
CTGGTGGATG AGCTGGATCT CACAGAGAAA CATAGAGCGG CCATGTTTGC CCTGCCTGCC 
GAGAAGAAAT GGCAAATCTA CTGTAGCAAG AAAAAGGAAC AGGAAGAGAA TAAAGGGGCG 
ACCAGCTGGC CTGAATTCTA CATCGATCAG ATCAATTCTA TGGCTGCTAG GAAGTCTTTG 
CTAGCTCTGG AGAAGGAGGA TGAGGAGGAG AGGAATAAAA CCATAGAGAG CCTGAAAACT 
GCTCTCCGCA CACAGCCCAT GAGGTTCGTG ACGCGCTTCA TAGACCAGGA TGGCCTGACG 
TGCATCTTAA ATTTTCTGAA AACGATGGAC TATGAGACGA CCGAATCGCA GATCCACACG 
TCGCTCATCG GCTGCATTAA AGCCCTGATG AACAACTCTC AGGGCCGAGC GCACGTGCTC 
TCGCATTCAG AAAGCATCAA CATCATTGCT CAAAGCCTGG CCACCGAGAA CATCAAGACT 
AAAGTGGCTG TTCTGGAGAT CATGGGTGCT GTTTGTTTGG TTCCTGGAGG ACACAAGAAA 
ATTCTGGAAG CCATGCTGCA TTATCAGAAG TTTGCCTGCG AGCGGACGCG CTTTCAGACT 
CTCCTGAATG ACTTGGACAG GAGTACGGGC CGCTACAGAG ATGAGGTGAA CCTCAAGACG 
GCCATCATGT CCTTCATCAA CGCCGTCCTC AGTCAAGGAG CAGGGGAGAC CAGTTTGGAG 
TTCAGAATTC ATTTGCGGTA TGAGTTTCTA ATGCTGGGCA TCCAACCAGT GATTGATAAA 
TTACGGTCCC ATGAAAACTC CACGTTAGAT CGGCATTTGG ACTTCTTTGA GATGCTACGA 
AATGAAGATG AATTAGCTCT CGCTAAGCGG TTTGAAAACG TTCATGTAGA TACTAAAAGT 
GCCACTCAGA TGTTCGAGCT CATCCGGAAG AGGATAAACC ACACAGACGC CTTCCCGCAC 
TTTATATCCG TCTTACAGCA CTGTCTTCAT ATGCCTTACA AGAAAACTGG GAACACTGTG 
CAGTACTGGG TTCTCCTAGA CAGAATTGTC CAGCAGATCA TCCTGCAGAA CGACAAGGGG 
CTCGACCCAG ATGTGGCACC GCTGGAGAAC TTTGATGTCA AAAACGTGGT GCGAATGCTG 
GTCAATGAGA ACGAGGTGAA ACAATGGAAA GAGCAAGCGG AAAAAATGAG GAAAGAACAT 
AATGAACTTC AGCAGAAGCT GGAAAAGAAA GAAAGGGAGT GTGATGCTAA AGCCCAAGAG 
AAGGAAGAAA TGATGCAAAC GCTCAATAAG ATGAAAGAGA AGCTGGAAAA GGAAAGCAGT 
GAACATAAGC TTGTTAAGCA GCAAGTGGCT GACCTTTCTG CCCGGCTACA TGAGATGAGT 
AATAGGACAA ATATCCCTGG TGGCCCTCCC CTCGCCCCTC TGGGTCCTGG TCTTCCACCT 
CCTCCTCCAC CTCCGGGTCA GGCTGGTCTT CCTCCTCCAC CTCCTCCAGG AGGAGCACCA 
CCACCACCTC CGCCTCCCCC CGGTGGACCT CCTCCTCCTC CAGGCCTGCT TGGCTTCGGT 
GCTCCTCCTC CTCCTGGAGG TCTCTTGGGC TCCACACTGA AGAAGAAGAA CATCCCGCAA 
CCATCCAACC CGCTCAAGTC TTTTAACTGG ACCAAACTCA GTGAAAATAA GCTGGAAGGG 
ACAGTGTGGC TGGATCTGGA CGACGTCAGA GTCTTCAAAC AATTAGATCT GGAGGATATT 
GAGAAAACAT TCTCTGCTTA TCAGCGACAG CAGGACTTTC TGTTCAATAA CTTTAGACAG 
AAAGAGTCCG AGGACGACAC TGTCACTTCT AAAAAGGTCA AAGAACTGTC TGTCATCGAT 
GGACGACGTG CGCAAAACTG CAACATCCTT CTCTCCAAGT TGAAACTGTC CAACGAGGAG 
ATCAAGAGAG CCATATTGAC CATGGATGAG CAGGAGGATC TGCCCAAAGA CATGCTGGAG 
CAGCTGTTGA AGTTTGTGCC AGAAAAAAGT GATGTAGACC TTCTGGAGGA GCACAAACAT 
GAGCTGGAGC GAATGGCGAA GGCAGACCGC TTCCTCTACG AGATGAGCAG AATCAACCAC 
TACCAGCAAA GACTGCAGTC TCTTTACTTT AAAAAGAAGT TTGCAGAAAG AATAGCAGAA 
ATCAAGCCCA AAGTGGAAGC TCTCAGTAAA GCATCTAAAG AGGTTCTTCA GAGCAAGAAT 
CTCCGTCAGC TTCTGGAGAT TGTCCTGGCC TTCGGAAACT ACATGAACAA AGGCCAGAGA 
GGCAATGCTT ACGGCTTCAA AGTGTCCTCT CTCAACAAGA TCGCTGACAC CAAATCCAGC 
ATCGACAAAA ACGTCACTCT TCTGCATTAC CTGATTACCG TTCTTGAGCA GAAATACCCC 
AAAGTCAGTC TGATTCACGA GGATCTGCAG AACGTTCCAG TTGCGGCCAA AGTCAACATG 
ACCGAACTGG AGAAAGACAT TAACAATCTG CGCTCTGGCT TGAAAAGCGT GGAGACGGAG 
CTGGAGTACC AGAGGACTCA ACCTCAGACC TATGGAGACA AGTTTGTGTC TGTAGTGAGT 
CAGTTCATCA CTGTGGCTGG ATTCAGTTTC TCTGACATTG AGGACTCTCT TCAGGATGCT 
AAGGATTCGT TTGGGAAGGC CGTGCAGCAC TTCGGTGAGG ACGCCACACG CATGCAGCCT 
GATGAGTTCT TCGGCATCTT CGATCAGTTC CTGCAGGGCT TCAGTGAAGC TAAACAAGAC 
AATGAGAACA TGAGACGACG GAAAGAGGAG GAGGAGCGCA GGGCCCGTAT GGAGGCACAG 
CTCAAGGAGC AGCGCGAGAG GGAAAGAAAA TCCCGGAAGG CTAAAGAAAA CTGCGAGGAG 
GACGGAGAGT TCGACGACCT GGTGTCTGCA CTGCGATCTG GGGAGGTGTT TGACAAAGAT 
ATGTCAAAGA TGAAACACAA CCGTAAACGT CCCGTCAAAT CGAGTGCAGA AAGCAGCAGA 
GAAAGACCCG TCACCAAACT CAACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAAM1A ( XM_702261.9 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_707353.4 Danio rerio dishevelled associated activator of morphogenesis 1a (daam1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa22320
Clone ID Related Accession (Same CDS sequence)	XM_702261.8 , XM_702261.9 , XM_017351771.2 , XM_017351771.1
Accession Version	XM_702261.9 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	disheveled-associated activator of morphogenesis 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_702261.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCC CACGTAAGCG AGGAGGTCGG GGCCTGTCAT TCCTCTGCTG CTGTCTAAAA 
GGGAGCGATC ATCCCGAAAT CACCTACAGC CTCCGTCATG GCAGCAACTT TACACTGCAG 
ACCATGGAGC CCACCCTGCC AATGCCCCCC ACTGAGGAGC TGGATGCTAT GTTCACTGAA 
CTGGTGGATG AGCTGGATCT CACAGAGAAA CATAGAGCGG CCATGTTTGC CCTGCCTGCC 
GAGAAGAAAT GGCAAATCTA CTGTAGCAAG AAAAAGGAAC AGGAAGAGAA TAAAGGGGCG 
ACCAGCTGGC CTGAATTCTA CATCGATCAG ATCAATTCTA TGGCTGCTAG GAAGTCTTTG 
CTAGCTCTGG AGAAGGAGGA TGAGGAGGAG AGGAATAAAA CCATAGAGAG CCTGAAAACT 
GCTCTCCGCA CACAGCCCAT GAGGTTCGTG ACGCGCTTCA TAGACCAGGA TGGCCTGACG 
TGCATCTTAA ATTTTCTGAA AACGATGGAC TATGAGACGA CCGAATCGCA GATCCACACG 
TCGCTCATCG GCTGCATTAA AGCCCTGATG AACAACTCTC AGGGCCGAGC GCACGTGCTC 
TCGCATTCAG AAAGCATCAA CATCATTGCT CAAAGCCTGG CCACCGAGAA CATCAAGACT 
AAAGTGGCTG TTCTGGAGAT CATGGGTGCT GTTTGTTTGG TTCCTGGAGG ACACAAGAAA 
ATTCTGGAAG CCATGCTGCA TTATCAGAAG TTTGCCTGCG AGCGGACGCG CTTTCAGACT 
CTCCTGAATG ACTTGGACAG GAGTACGGGC CGCTACAGAG ATGAGGTGAA CCTCAAGACG 
GCCATCATGT CCTTCATCAA CGCCGTCCTC AGTCAAGGAG CAGGGGAGAC CAGTTTGGAG 
TTCAGAATTC ATTTGCGGTA TGAGTTTCTA ATGCTGGGCA TCCAACCAGT GATTGATAAA 
TTACGGTCCC ATGAAAACTC CACGTTAGAT CGGCATTTGG ACTTCTTTGA GATGCTACGA 
AATGAAGATG AATTAGCTCT CGCTAAGCGG TTTGAAAACG TTCATGTAGA TACTAAAAGT 
GCCACTCAGA TGTTCGAGCT CATCCGGAAG AGGATAAACC ACACAGACGC CTTCCCGCAC 
TTTATATCCG TCTTACAGCA CTGTCTTCAT ATGCCTTACA AGAAAACTGG GAACACTGTG 
CAGTACTGGG TTCTCCTAGA CAGAATTGTC CAGCAGATCA TCCTGCAGAA CGACAAGGGG 
CTCGACCCAG ATGTGGCACC GCTGGAGAAC TTTGATGTCA AAAACGTGGT GCGAATGCTG 
GTCAATGAGA ACGAGGTGAA ACAATGGAAA GAGCAAGCGG AAAAAATGAG GAAAGAACAT 
AATGAACTTC AGCAGAAGCT GGAAAAGAAA GAAAGGGAGT GTGATGCTAA AGCCCAAGAG 
AAGGAAGAAA TGATGCAAAC GCTCAATAAG ATGAAAGAGA AGCTGGAAAA GGAAAGCAGT 
GAACATAAGC TTGTTAAGCA GCAAGTGGCT GACCTTTCTG CCCGGCTACA TGAGATGAGT 
AATAGGACAA ATATCCCTGG TGGCCCTCCC CTCGCCCCTC TGGGTCCTGG TCTTCCACCT 
CCTCCTCCAC CTCCGGGTCA GGCTGGTCTT CCTCCTCCAC CTCCTCCAGG AGGAGCACCA 
CCACCACCTC CGCCTCCCCC CGGTGGACCT CCTCCTCCTC CAGGCCTGCT TGGCTTCGGT 
GCTCCTCCTC CTCCTGGAGG TCTCTTGGGC TCCACACTGA AGAAGAAGAA CATCCCGCAA 
CCATCCAACC CGCTCAAGTC TTTTAACTGG ACCAAACTCA GTGAAAATAA GCTGGAAGGG 
ACAGTGTGGC TGGATCTGGA CGACGTCAGA GTCTTCAAAC AATTAGATCT GGAGGATATT 
GAGAAAACAT TCTCTGCTTA TCAGCGACAG CAGGACTTTC TGTTCAATAA CTTTAGACAG 
AAAGAGTCCG AGGACGACAC TGTCACTTCT AAAAAGGTCA AAGAACTGTC TGTCATCGAT 
GGACGACGTG CGCAAAACTG CAACATCCTT CTCTCCAAGT TGAAACTGTC CAACGAGGAG 
ATCAAGAGAG CCATATTGAC CATGGATGAG CAGGAGGATC TGCCCAAAGA CATGCTGGAG 
CAGCTGTTGA AGTTTGTGCC AGAAAAAAGT GATGTAGACC TTCTGGAGGA GCACAAACAT 
GAGCTGGAGC GAATGGCGAA GGCAGACCGC TTCCTCTACG AGATGAGCAG AATCAACCAC 
TACCAGCAAA GACTGCAGTC TCTTTACTTT AAAAAGAAGT TTGCAGAAAG AATAGCAGAA 
ATCAAGCCCA AAGTGGAAGC TCTCAGTAAA GCATCTAAAG AGGTTCTTCA GAGCAAGAAT 
CTCCGTCAGC TTCTGGAGAT TGTCCTGGCC TTCGGAAACT ACATGAACAA AGGCCAGAGA 
GGCAATGCTT ACGGCTTCAA AGTGTCCTCT CTCAACAAGA TCGCTGACAC CAAATCCAGC 
ATCGACAAAA ACGTCACTCT TCTGCATTAC CTGATTACCG TTCTTGAGCA GAAATACCCC 
AAAGTCAGTC TGATTCACGA GGATCTGCAG AACGTTCCAG TTGCGGCCAA AGTCAACATG 
ACCGAACTGG AGAAAGACAT TAACAATCTG CGCTCTGGCT TGAAAAGCGT GGAGACGGAG 
CTGGAGTACC AGAGGACTCA ACCTCAGACC TATGGAGACA AGTTTGTGTC TGTAGTGAGT 
CAGTTCATCA CTGTGGCTGG ATTCAGTTTC TCTGACATTG AGGACTCTCT TCAGGATGCT 
AAGGATTCGT TTGGGAAGGC CGTGCAGCAC TTCGGTGAGG ACGCCACACG CATGCAGCCT 
GATGAGTTCT TCGGCATCTT CGATCAGTTC CTGCAGGGCT TCAGTGAAGC TAAACAAGAC 
AATGAGAACA TGAGACGACG GAAAGAGGAG GAGGAGCGCA GGGCCCGTAT GGAGGCACAG 
CTCAAGGAGC AGCGCGAGAG GGAAAGAAAA TCCCGGAAGG CTAAAGAAAA CTGCGAGGAG 
GACGGAGAGT TCGACGACCT GGTGTCTGCA CTGCGATCTG GGGAGGTGTT TGACAAAGAT 
ATGTCAAAGA TGAAACACAA CCGTAAACGT CCCGTCAAAT CGAGTGCAGA AAGCAGCAGA 
GAAAGACCCG TCACCAAACT CAACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_707353.4
CDS	416..3625
Translation	MMAPRKRGGRGLSFLCCCLKGSDHPEITYSLRHGSNFTLQTMEPTLPMPPTEELDAMFTELVDELDLTEKHRAAMFALPAEKKWQIYCSKKKEQEENKGATSWPEFYIDQINSMAARKSLLALEKEDEEERNKTIESLKTALRTQPMRFVTRFIDQDGLTCILNFLKTMDYETTESQIHTSLIGCIKALMNNSQGRAHVLSHSESINIIAQSLATENIKTKVAVLEIMGAVCLVPGGHKKILEAMLHYQKFACERTRFQTLLNDLDRSTGRYRDEVNLKTAIMSFINAVLSQGAGETSLEFRIHLRYEFLMLGIQPVIDKLRSHENSTLDRHLDFFEMLRNEDELALAKRFENVHVDTKSATQMFELIRKRINHTDAFPHFISVLQHCLHMPYKKTGNTVQYWVLLDRIVQQIILQNDKGLDPDVAPLENFDVKNVVRMLVNENEVKQWKEQAEKMRKEHNELQQKLEKKERECDAKAQEKEEMMQTLNKMKEKLEKESSEHKLVKQQVADLSARLHEMSNRTNIPGGPPLAPLGPGLPPPPPPPGQAGLPPPPPPGGAPPPPPPPPGGPPPPPGLLGFGAPPPPGGLLGSTLKKKNIPQPSNPLKSFNWTKLSENKLEGTVWLDLDDVRVFKQLDLEDIEKTFSAYQRQQDFLFNNFRQKESEDDTVTSKKVKELSVIDGRRAQNCNILLSKLKLSNEEIKRAILTMDEQEDLPKDMLEQLLKFVPEKSDVDLLEEHKHELERMAKADRFLYEMSRINHYQQRLQSLYFKKKFAERIAEIKPKVEALSKASKEVLQSKNLRQLLEIVLAFGNYMNKGQRGNAYGFKVSSLNKIADTKSSIDKNVTLLHYLITVLEQKYPKVSLIHEDLQNVPVAAKVNMTELEKDINNLRSGLKSVETELEYQRTQPQTYGDKFVSVVSQFITVAGFSFSDIEDSLQDAKDSFGKAVQHFGEDATRMQPDEFFGIFDQFLQGFSEAKQDNENMRRRKEEEERRARMEAQLKEQRERERKSRKAKENCEEDGEFDDLVSALRSGEVFDKDMSKMKHNRKRPVKSSAESSRERPVTKLNF

Target ORF information:

RefSeq Version	XM_702261.9
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dishevelled associated activator of morphogenesis 1a (daam1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_702261.9

ORF Insert Sequence:

ATGATGGCCC CACGTAAGCG AGGAGGTCGG GGCCTGTCAT TCCTCTGCTG CTGTCTAAAA 
GGGAGCGATC ATCCCGAAAT CACCTACAGC CTCCGTCATG GCAGCAACTT TACACTGCAG 
ACCATGGAGC CCACCCTGCC AATGCCCCCC ACTGAGGAGC TGGATGCTAT GTTCACTGAA 
CTGGTGGATG AGCTGGATCT CACAGAGAAA CATAGAGCGG CCATGTTTGC CCTGCCTGCC 
GAGAAGAAAT GGCAAATCTA CTGTAGCAAG AAAAAGGAAC AGGAAGAGAA TAAAGGGGCG 
ACCAGCTGGC CTGAATTCTA CATCGATCAG ATCAATTCTA TGGCTGCTAG GAAGTCTTTG 
CTAGCTCTGG AGAAGGAGGA TGAGGAGGAG AGGAATAAAA CCATAGAGAG CCTGAAAACT 
GCTCTCCGCA CACAGCCCAT GAGGTTCGTG ACGCGCTTCA TAGACCAGGA TGGCCTGACG 
TGCATCTTAA ATTTTCTGAA AACGATGGAC TATGAGACGA CCGAATCGCA GATCCACACG 
TCGCTCATCG GCTGCATTAA AGCCCTGATG AACAACTCTC AGGGCCGAGC GCACGTGCTC 
TCGCATTCAG AAAGCATCAA CATCATTGCT CAAAGCCTGG CCACCGAGAA CATCAAGACT 
AAAGTGGCTG TTCTGGAGAT CATGGGTGCT GTTTGTTTGG TTCCTGGAGG ACACAAGAAA 
ATTCTGGAAG CCATGCTGCA TTATCAGAAG TTTGCCTGCG AGCGGACGCG CTTTCAGACT 
CTCCTGAATG ACTTGGACAG GAGTACGGGC CGCTACAGAG ATGAGGTGAA CCTCAAGACG 
GCCATCATGT CCTTCATCAA CGCCGTCCTC AGTCAAGGAG CAGGGGAGAC CAGTTTGGAG 
TTCAGAATTC ATTTGCGGTA TGAGTTTCTA ATGCTGGGCA TCCAACCAGT GATTGATAAA 
TTACGGTCCC ATGAAAACTC CACGTTAGAT CGGCATTTGG ACTTCTTTGA GATGCTACGA 
AATGAAGATG AATTAGCTCT CGCTAAGCGG TTTGAAAACG TTCATGTAGA TACTAAAAGT 
GCCACTCAGA TGTTCGAGCT CATCCGGAAG AGGATAAACC ACACAGACGC CTTCCCGCAC 
TTTATATCCG TCTTACAGCA CTGTCTTCAT ATGCCTTACA AGAAAACTGG GAACACTGTG 
CAGTACTGGG TTCTCCTAGA CAGAATTGTC CAGCAGATCA TCCTGCAGAA CGACAAGGGG 
CTCGACCCAG ATGTGGCACC GCTGGAGAAC TTTGATGTCA AAAACGTGGT GCGAATGCTG 
GTCAATGAGA ACGAGGTGAA ACAATGGAAA GAGCAAGCGG AAAAAATGAG GAAAGAACAT 
AATGAACTTC AGCAGAAGCT GGAAAAGAAA GAAAGGGAGT GTGATGCTAA AGCCCAAGAG 
AAGGAAGAAA TGATGCAAAC GCTCAATAAG ATGAAAGAGA AGCTGGAAAA GGAAAGCAGT 
GAACATAAGC TTGTTAAGCA GCAAGTGGCT GACCTTTCTG CCCGGCTACA TGAGATGAGT 
AATAGGACAA ATATCCCTGG TGGCCCTCCC CTCGCCCCTC TGGGTCCTGG TCTTCCACCT 
CCTCCTCCAC CTCCGGGTCA GGCTGGTCTT CCTCCTCCAC CTCCTCCAGG AGGAGCACCA 
CCACCACCTC CGCCTCCCCC CGGTGGACCT CCTCCTCCTC CAGGCCTGCT TGGCTTCGGT 
GCTCCTCCTC CTCCTGGAGG TCTCTTGGGC TCCACACTGA AGAAGAAGAA CATCCCGCAA 
CCATCCAACC CGCTCAAGTC TTTTAACTGG ACCAAACTCA GTGAAAATAA GCTGGAAGGG 
ACAGTGTGGC TGGATCTGGA CGACGTCAGA GTCTTCAAAC AATTAGATCT GGAGGATATT 
GAGAAAACAT TCTCTGCTTA TCAGCGACAG CAGGACTTTC TGTTCAATAA CTTTAGACAG 
AAAGAGTCCG AGGACGACAC TGTCACTTCT AAAAAGGTCA AAGAACTGTC TGTCATCGAT 
GGACGACGTG CGCAAAACTG CAACATCCTT CTCTCCAAGT TGAAACTGTC CAACGAGGAG 
ATCAAGAGAG CCATATTGAC CATGGATGAG CAGGAGGATC TGCCCAAAGA CATGCTGGAG 
CAGCTGTTGA AGTTTGTGCC AGAAAAAAGT GATGTAGACC TTCTGGAGGA GCACAAACAT 
GAGCTGGAGC GAATGGCGAA GGCAGACCGC TTCCTCTACG AGATGAGCAG AATCAACCAC 
TACCAGCAAA GACTGCAGTC TCTTTACTTT AAAAAGAAGT TTGCAGAAAG AATAGCAGAA 
ATCAAGCCCA AAGTGGAAGC TCTCAGTAAA GCATCTAAAG AGGTTCTTCA GAGCAAGAAT 
CTCCGTCAGC TTCTGGAGAT TGTCCTGGCC TTCGGAAACT ACATGAACAA AGGCCAGAGA 
GGCAATGCTT ACGGCTTCAA AGTGTCCTCT CTCAACAAGA TCGCTGACAC CAAATCCAGC 
ATCGACAAAA ACGTCACTCT TCTGCATTAC CTGATTACCG TTCTTGAGCA GAAATACCCC 
AAAGTCAGTC TGATTCACGA GGATCTGCAG AACGTTCCAG TTGCGGCCAA AGTCAACATG 
ACCGAACTGG AGAAAGACAT TAACAATCTG CGCTCTGGCT TGAAAAGCGT GGAGACGGAG 
CTGGAGTACC AGAGGACTCA ACCTCAGACC TATGGAGACA AGTTTGTGTC TGTAGTGAGT 
CAGTTCATCA CTGTGGCTGG ATTCAGTTTC TCTGACATTG AGGACTCTCT TCAGGATGCT 
AAGGATTCGT TTGGGAAGGC CGTGCAGCAC TTCGGTGAGG ACGCCACACG CATGCAGCCT 
GATGAGTTCT TCGGCATCTT CGATCAGTTC CTGCAGGGCT TCAGTGAAGC TAAACAAGAC 
AATGAGAACA TGAGACGACG GAAAGAGGAG GAGGAGCGCA GGGCCCGTAT GGAGGCACAG 
CTCAAGGAGC AGCGCGAGAG GGAAAGAAAA TCCCGGAAGG CTAAAGAAAA CTGCGAGGAG 
GACGGAGAGT TCGACGACCT GGTGTCTGCA CTGCGATCTG GGGAGGTGTT TGACAAAGAT 
ATGTCAAAGA TGAAACACAA CCGTAAACGT CCCGTCAAAT CGAGTGCAGA AAGCAGCAGA 
GAAAGACCCG TCACCAAACT CAACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAAM1A ( XM_017351771.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017207260.1 Danio rerio dishevelled associated activator of morphogenesis 1a (daam1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa22320
Clone ID Related Accession (Same CDS sequence)	XM_702261.8 , XM_702261.9 , XM_017351771.2 , XM_017351771.1
Accession Version	XM_017351771.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	disheveled-associated activator of morphogenesis 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017351771.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCC CACGTAAGCG AGGAGGTCGG GGCCTGTCAT TCCTCTGCTG CTGTCTAAAA 
GGGAGCGATC ATCCCGAAAT CACCTACAGC CTCCGTCATG GCAGCAACTT TACACTGCAG 
ACCATGGAGC CCACCCTGCC AATGCCCCCC ACTGAGGAGC TGGATGCTAT GTTCACTGAA 
CTGGTGGATG AGCTGGATCT CACAGAGAAA CATAGAGCGG CCATGTTTGC CCTGCCTGCC 
GAGAAGAAAT GGCAAATCTA CTGTAGCAAG AAAAAGGAAC AGGAAGAGAA TAAAGGGGCG 
ACCAGCTGGC CTGAATTCTA CATCGATCAG ATCAATTCTA TGGCTGCTAG GAAGTCTTTG 
CTAGCTCTGG AGAAGGAGGA TGAGGAGGAG AGGAATAAAA CCATAGAGAG CCTGAAAACT 
GCTCTCCGCA CACAGCCCAT GAGGTTCGTG ACGCGCTTCA TAGACCAGGA TGGCCTGACG 
TGCATCTTAA ATTTTCTGAA AACGATGGAC TATGAGACGA CCGAATCGCA GATCCACACG 
TCGCTCATCG GCTGCATTAA AGCCCTGATG AACAACTCTC AGGGCCGAGC GCACGTGCTC 
TCGCATTCAG AAAGCATCAA CATCATTGCT CAAAGCCTGG CCACCGAGAA CATCAAGACT 
AAAGTGGCTG TTCTGGAGAT CATGGGTGCT GTTTGTTTGG TTCCTGGAGG ACACAAGAAA 
ATTCTGGAAG CCATGCTGCA TTATCAGAAG TTTGCCTGCG AGCGGACGCG CTTTCAGACT 
CTCCTGAATG ACTTGGACAG GAGTACGGGC CGCTACAGAG ATGAGGTGAA CCTCAAGACG 
GCCATCATGT CCTTCATCAA CGCCGTCCTC AGTCAAGGAG CAGGGGAGAC CAGTTTGGAG 
TTCAGAATTC ATTTGCGGTA TGAGTTTCTA ATGCTGGGCA TCCAACCAGT GATTGATAAA 
TTACGGTCCC ATGAAAACTC CACGTTAGAT CGGCATTTGG ACTTCTTTGA GATGCTACGA 
AATGAAGATG AATTAGCTCT CGCTAAGCGG TTTGAAAACG TTCATGTAGA TACTAAAAGT 
GCCACTCAGA TGTTCGAGCT CATCCGGAAG AGGATAAACC ACACAGACGC CTTCCCGCAC 
TTTATATCCG TCTTACAGCA CTGTCTTCAT ATGCCTTACA AGAAAACTGG GAACACTGTG 
CAGTACTGGG TTCTCCTAGA CAGAATTGTC CAGCAGATCA TCCTGCAGAA CGACAAGGGG 
CTCGACCCAG ATGTGGCACC GCTGGAGAAC TTTGATGTCA AAAACGTGGT GCGAATGCTG 
GTCAATGAGA ACGAGGTGAA ACAATGGAAA GAGCAAGCGG AAAAAATGAG GAAAGAACAT 
AATGAACTTC AGCAGAAGCT GGAAAAGAAA GAAAGGGAGT GTGATGCTAA AGCCCAAGAG 
AAGGAAGAAA TGATGCAAAC GCTCAATAAG ATGAAAGAGA AGCTGGAAAA GGAAAGCAGT 
GAACATAAGC TTGTTAAGCA GCAAGTGGCT GACCTTTCTG CCCGGCTACA TGAGATGAGT 
AATAGGACAA ATATCCCTGG TGGCCCTCCC CTCGCCCCTC TGGGTCCTGG TCTTCCACCT 
CCTCCTCCAC CTCCGGGTCA GGCTGGTCTT CCTCCTCCAC CTCCTCCAGG AGGAGCACCA 
CCACCACCTC CGCCTCCCCC CGGTGGACCT CCTCCTCCTC CAGGCCTGCT TGGCTTCGGT 
GCTCCTCCTC CTCCTGGAGG TCTCTTGGGC TCCACACTGA AGAAGAAGAA CATCCCGCAA 
CCATCCAACC CGCTCAAGTC TTTTAACTGG ACCAAACTCA GTGAAAATAA GCTGGAAGGG 
ACAGTGTGGC TGGATCTGGA CGACGTCAGA GTCTTCAAAC AATTAGATCT GGAGGATATT 
GAGAAAACAT TCTCTGCTTA TCAGCGACAG CAGGACTTTC TGTTCAATAA CTTTAGACAG 
AAAGAGTCCG AGGACGACAC TGTCACTTCT AAAAAGGTCA AAGAACTGTC TGTCATCGAT 
GGACGACGTG CGCAAAACTG CAACATCCTT CTCTCCAAGT TGAAACTGTC CAACGAGGAG 
ATCAAGAGAG CCATATTGAC CATGGATGAG CAGGAGGATC TGCCCAAAGA CATGCTGGAG 
CAGCTGTTGA AGTTTGTGCC AGAAAAAAGT GATGTAGACC TTCTGGAGGA GCACAAACAT 
GAGCTGGAGC GAATGGCGAA GGCAGACCGC TTCCTCTACG AGATGAGCAG AATCAACCAC 
TACCAGCAAA GACTGCAGTC TCTTTACTTT AAAAAGAAGT TTGCAGAAAG AATAGCAGAA 
ATCAAGCCCA AAGTGGAAGC TCTCAGTAAA GCATCTAAAG AGGTTCTTCA GAGCAAGAAT 
CTCCGTCAGC TTCTGGAGAT TGTCCTGGCC TTCGGAAACT ACATGAACAA AGGCCAGAGA 
GGCAATGCTT ACGGCTTCAA AGTGTCCTCT CTCAACAAGA TCGCTGACAC CAAATCCAGC 
ATCGACAAAA ACGTCACTCT TCTGCATTAC CTGATTACCG TTCTTGAGCA GAAATACCCC 
AAAGTCAGTC TGATTCACGA GGATCTGCAG AACGTTCCAG TTGCGGCCAA AGTCAACATG 
ACCGAACTGG AGAAAGACAT TAACAATCTG CGCTCTGGCT TGAAAAGCGT GGAGACGGAG 
CTGGAGTACC AGAGGACTCA ACCTCAGACC TATGGAGACA AGTTTGTGTC TGTAGTGAGT 
CAGTTCATCA CTGTGGCTGG ATTCAGTTTC TCTGACATTG AGGACTCTCT TCAGGATGCT 
AAGGATTCGT TTGGGAAGGC CGTGCAGCAC TTCGGTGAGG ACGCCACACG CATGCAGCCT 
GATGAGTTCT TCGGCATCTT CGATCAGTTC CTGCAGGGCT TCAGTGAAGC TAAACAAGAC 
AATGAGAACA TGAGACGACG GAAAGAGGAG GAGGAGCGCA GGGCCCGTAT GGAGGCACAG 
CTCAAGGAGC AGCGCGAGAG GGAAAGAAAA TCCCGGAAGG CTAAAGAAAA CTGCGAGGAG 
GACGGAGAGT TCGACGACCT GGTGTCTGCA CTGCGATCTG GGGAGGTGTT TGACAAAGAT 
ATGTCAAAGA TGAAACACAA CCGTAAACGT CCCGTCAAAT CGAGTGCAGA AAGCAGCAGA 
GAAAGACCCG TCACCAAACT CAACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207260.1
CDS	166..3375
Translation	MMAPRKRGGRGLSFLCCCLKGSDHPEITYSLRHGSNFTLQTMEPTLPMPPTEELDAMFTELVDELDLTEKHRAAMFALPAEKKWQIYCSKKKEQEENKGATSWPEFYIDQINSMAARKSLLALEKEDEEERNKTIESLKTALRTQPMRFVTRFIDQDGLTCILNFLKTMDYETTESQIHTSLIGCIKALMNNSQGRAHVLSHSESINIIAQSLATENIKTKVAVLEIMGAVCLVPGGHKKILEAMLHYQKFACERTRFQTLLNDLDRSTGRYRDEVNLKTAIMSFINAVLSQGAGETSLEFRIHLRYEFLMLGIQPVIDKLRSHENSTLDRHLDFFEMLRNEDELALAKRFENVHVDTKSATQMFELIRKRINHTDAFPHFISVLQHCLHMPYKKTGNTVQYWVLLDRIVQQIILQNDKGLDPDVAPLENFDVKNVVRMLVNENEVKQWKEQAEKMRKEHNELQQKLEKKERECDAKAQEKEEMMQTLNKMKEKLEKESSEHKLVKQQVADLSARLHEMSNRTNIPGGPPLAPLGPGLPPPPPPPGQAGLPPPPPPGGAPPPPPPPPGGPPPPPGLLGFGAPPPPGGLLGSTLKKKNIPQPSNPLKSFNWTKLSENKLEGTVWLDLDDVRVFKQLDLEDIEKTFSAYQRQQDFLFNNFRQKESEDDTVTSKKVKELSVIDGRRAQNCNILLSKLKLSNEEIKRAILTMDEQEDLPKDMLEQLLKFVPEKSDVDLLEEHKHELERMAKADRFLYEMSRINHYQQRLQSLYFKKKFAERIAEIKPKVEALSKASKEVLQSKNLRQLLEIVLAFGNYMNKGQRGNAYGFKVSSLNKIADTKSSIDKNVTLLHYLITVLEQKYPKVSLIHEDLQNVPVAAKVNMTELEKDINNLRSGLKSVETELEYQRTQPQTYGDKFVSVVSQFITVAGFSFSDIEDSLQDAKDSFGKAVQHFGEDATRMQPDEFFGIFDQFLQGFSEAKQDNENMRRRKEEEERRARMEAQLKEQRERERKSRKAKENCEEDGEFDDLVSALRSGEVFDKDMSKMKHNRKRPVKSSAESSRERPVTKLNF

Target ORF information:

RefSeq Version	XM_017351771.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dishevelled associated activator of morphogenesis 1a (daam1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351771.2

ORF Insert Sequence:

ATGATGGCCC CACGTAAGCG AGGAGGTCGG GGCCTGTCAT TCCTCTGCTG CTGTCTAAAA 
GGGAGCGATC ATCCCGAAAT CACCTACAGC CTCCGTCATG GCAGCAACTT TACACTGCAG 
ACCATGGAGC CCACCCTGCC AATGCCCCCC ACTGAGGAGC TGGATGCTAT GTTCACTGAA 
CTGGTGGATG AGCTGGATCT CACAGAGAAA CATAGAGCGG CCATGTTTGC CCTGCCTGCC 
GAGAAGAAAT GGCAAATCTA CTGTAGCAAG AAAAAGGAAC AGGAAGAGAA TAAAGGGGCG 
ACCAGCTGGC CTGAATTCTA CATCGATCAG ATCAATTCTA TGGCTGCTAG GAAGTCTTTG 
CTAGCTCTGG AGAAGGAGGA TGAGGAGGAG AGGAATAAAA CCATAGAGAG CCTGAAAACT 
GCTCTCCGCA CACAGCCCAT GAGGTTCGTG ACGCGCTTCA TAGACCAGGA TGGCCTGACG 
TGCATCTTAA ATTTTCTGAA AACGATGGAC TATGAGACGA CCGAATCGCA GATCCACACG 
TCGCTCATCG GCTGCATTAA AGCCCTGATG AACAACTCTC AGGGCCGAGC GCACGTGCTC 
TCGCATTCAG AAAGCATCAA CATCATTGCT CAAAGCCTGG CCACCGAGAA CATCAAGACT 
AAAGTGGCTG TTCTGGAGAT CATGGGTGCT GTTTGTTTGG TTCCTGGAGG ACACAAGAAA 
ATTCTGGAAG CCATGCTGCA TTATCAGAAG TTTGCCTGCG AGCGGACGCG CTTTCAGACT 
CTCCTGAATG ACTTGGACAG GAGTACGGGC CGCTACAGAG ATGAGGTGAA CCTCAAGACG 
GCCATCATGT CCTTCATCAA CGCCGTCCTC AGTCAAGGAG CAGGGGAGAC CAGTTTGGAG 
TTCAGAATTC ATTTGCGGTA TGAGTTTCTA ATGCTGGGCA TCCAACCAGT GATTGATAAA 
TTACGGTCCC ATGAAAACTC CACGTTAGAT CGGCATTTGG ACTTCTTTGA GATGCTACGA 
AATGAAGATG AATTAGCTCT CGCTAAGCGG TTTGAAAACG TTCATGTAGA TACTAAAAGT 
GCCACTCAGA TGTTCGAGCT CATCCGGAAG AGGATAAACC ACACAGACGC CTTCCCGCAC 
TTTATATCCG TCTTACAGCA CTGTCTTCAT ATGCCTTACA AGAAAACTGG GAACACTGTG 
CAGTACTGGG TTCTCCTAGA CAGAATTGTC CAGCAGATCA TCCTGCAGAA CGACAAGGGG 
CTCGACCCAG ATGTGGCACC GCTGGAGAAC TTTGATGTCA AAAACGTGGT GCGAATGCTG 
GTCAATGAGA ACGAGGTGAA ACAATGGAAA GAGCAAGCGG AAAAAATGAG GAAAGAACAT 
AATGAACTTC AGCAGAAGCT GGAAAAGAAA GAAAGGGAGT GTGATGCTAA AGCCCAAGAG 
AAGGAAGAAA TGATGCAAAC GCTCAATAAG ATGAAAGAGA AGCTGGAAAA GGAAAGCAGT 
GAACATAAGC TTGTTAAGCA GCAAGTGGCT GACCTTTCTG CCCGGCTACA TGAGATGAGT 
AATAGGACAA ATATCCCTGG TGGCCCTCCC CTCGCCCCTC TGGGTCCTGG TCTTCCACCT 
CCTCCTCCAC CTCCGGGTCA GGCTGGTCTT CCTCCTCCAC CTCCTCCAGG AGGAGCACCA 
CCACCACCTC CGCCTCCCCC CGGTGGACCT CCTCCTCCTC CAGGCCTGCT TGGCTTCGGT 
GCTCCTCCTC CTCCTGGAGG TCTCTTGGGC TCCACACTGA AGAAGAAGAA CATCCCGCAA 
CCATCCAACC CGCTCAAGTC TTTTAACTGG ACCAAACTCA GTGAAAATAA GCTGGAAGGG 
ACAGTGTGGC TGGATCTGGA CGACGTCAGA GTCTTCAAAC AATTAGATCT GGAGGATATT 
GAGAAAACAT TCTCTGCTTA TCAGCGACAG CAGGACTTTC TGTTCAATAA CTTTAGACAG 
AAAGAGTCCG AGGACGACAC TGTCACTTCT AAAAAGGTCA AAGAACTGTC TGTCATCGAT 
GGACGACGTG CGCAAAACTG CAACATCCTT CTCTCCAAGT TGAAACTGTC CAACGAGGAG 
ATCAAGAGAG CCATATTGAC CATGGATGAG CAGGAGGATC TGCCCAAAGA CATGCTGGAG 
CAGCTGTTGA AGTTTGTGCC AGAAAAAAGT GATGTAGACC TTCTGGAGGA GCACAAACAT 
GAGCTGGAGC GAATGGCGAA GGCAGACCGC TTCCTCTACG AGATGAGCAG AATCAACCAC 
TACCAGCAAA GACTGCAGTC TCTTTACTTT AAAAAGAAGT TTGCAGAAAG AATAGCAGAA 
ATCAAGCCCA AAGTGGAAGC TCTCAGTAAA GCATCTAAAG AGGTTCTTCA GAGCAAGAAT 
CTCCGTCAGC TTCTGGAGAT TGTCCTGGCC TTCGGAAACT ACATGAACAA AGGCCAGAGA 
GGCAATGCTT ACGGCTTCAA AGTGTCCTCT CTCAACAAGA TCGCTGACAC CAAATCCAGC 
ATCGACAAAA ACGTCACTCT TCTGCATTAC CTGATTACCG TTCTTGAGCA GAAATACCCC 
AAAGTCAGTC TGATTCACGA GGATCTGCAG AACGTTCCAG TTGCGGCCAA AGTCAACATG 
ACCGAACTGG AGAAAGACAT TAACAATCTG CGCTCTGGCT TGAAAAGCGT GGAGACGGAG 
CTGGAGTACC AGAGGACTCA ACCTCAGACC TATGGAGACA AGTTTGTGTC TGTAGTGAGT 
CAGTTCATCA CTGTGGCTGG ATTCAGTTTC TCTGACATTG AGGACTCTCT TCAGGATGCT 
AAGGATTCGT TTGGGAAGGC CGTGCAGCAC TTCGGTGAGG ACGCCACACG CATGCAGCCT 
GATGAGTTCT TCGGCATCTT CGATCAGTTC CTGCAGGGCT TCAGTGAAGC TAAACAAGAC 
AATGAGAACA TGAGACGACG GAAAGAGGAG GAGGAGCGCA GGGCCCGTAT GGAGGCACAG 
CTCAAGGAGC AGCGCGAGAG GGAAAGAAAA TCCCGGAAGG CTAAAGAAAA CTGCGAGGAG 
GACGGAGAGT TCGACGACCT GGTGTCTGCA CTGCGATCTG GGGAGGTGTT TGACAAAGAT 
ATGTCAAAGA TGAAACACAA CCGTAAACGT CCCGTCAAAT CGAGTGCAGA AAGCAGCAGA 
GAAAGACCCG TCACCAAACT CAACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAAM1A ( XM_017351771.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017207260.1 Danio rerio dishevelled associated activator of morphogenesis 1a (daam1a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa22320
Clone ID Related Accession (Same CDS sequence)	XM_702261.8 , XM_702261.9 , XM_017351771.2 , XM_017351771.1
Accession Version	XM_017351771.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3210bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	disheveled-associated activator of morphogenesis 1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCC CACGTAAGCG AGGAGGTCGG GGCCTGTCAT TCCTCTGCTG CTGTCTAAAA 
GGGAGCGATC ATCCCGAAAT CACCTACAGC CTCCGTCATG GCAGCAACTT TACACTGCAG 
ACCATGGAGC CCACCCTGCC AATGCCCCCC ACTGAGGAGC TGGATGCTAT GTTCACTGAA 
CTGGTGGATG AGCTGGATCT CACAGAGAAA CATAGAGCGG CCATGTTTGC CCTGCCTGCC 
GAGAAGAAAT GGCAAATCTA CTGTAGCAAG AAAAAGGAAC AGGAAGAGAA TAAAGGGGCG 
ACCAGCTGGC CTGAATTCTA CATCGATCAG ATCAATTCTA TGGCTGCTAG GAAGTCTTTG 
CTAGCTCTGG AGAAGGAGGA TGAGGAGGAG AGGAATAAAA CCATAGAGAG CCTGAAAACT 
GCTCTCCGCA CACAGCCCAT GAGGTTCGTG ACGCGCTTCA TAGACCAGGA TGGCCTGACG 
TGCATCTTAA ATTTTCTGAA AACGATGGAC TATGAGACGA CCGAATCGCA GATCCACACG 
TCGCTCATCG GCTGCATTAA AGCCCTGATG AACAACTCTC AGGGCCGAGC GCACGTGCTC 
TCGCATTCAG AAAGCATCAA CATCATTGCT CAAAGCCTGG CCACCGAGAA CATCAAGACT 
AAAGTGGCTG TTCTGGAGAT CATGGGTGCT GTTTGTTTGG TTCCTGGAGG ACACAAGAAA 
ATTCTGGAAG CCATGCTGCA TTATCAGAAG TTTGCCTGCG AGCGGACGCG CTTTCAGACT 
CTCCTGAATG ACTTGGACAG GAGTACGGGC CGCTACAGAG ATGAGGTGAA CCTCAAGACG 
GCCATCATGT CCTTCATCAA CGCCGTCCTC AGTCAAGGAG CAGGGGAGAC CAGTTTGGAG 
TTCAGAATTC ATTTGCGGTA TGAGTTTCTA ATGCTGGGCA TCCAACCAGT GATTGATAAA 
TTACGGTCCC ATGAAAACTC CACGTTAGAT CGGCATTTGG ACTTCTTTGA GATGCTACGA 
AATGAAGATG AATTAGCTCT CGCTAAGCGG TTTGAAAACG TTCATGTAGA TACTAAAAGT 
GCCACTCAGA TGTTCGAGCT CATCCGGAAG AGGATAAACC ACACAGACGC CTTCCCGCAC 
TTTATATCCG TCTTACAGCA CTGTCTTCAT ATGCCTTACA AGAAAACTGG GAACACTGTG 
CAGTACTGGG TTCTCCTAGA CAGAATTGTC CAGCAGATCA TCCTGCAGAA CGACAAGGGG 
CTCGACCCAG ATGTGGCACC GCTGGAGAAC TTTGATGTCA AAAACGTGGT GCGAATGCTG 
GTCAATGAGA ACGAGGTGAA ACAATGGAAA GAGCAAGCGG AAAAAATGAG GAAAGAACAT 
AATGAACTTC AGCAGAAGCT GGAAAAGAAA GAAAGGGAGT GTGATGCTAA AGCCCAAGAG 
AAGGAAGAAA TGATGCAAAC GCTCAATAAG ATGAAAGAGA AGCTGGAAAA GGAAAGCAGT 
GAACATAAGC TTGTTAAGCA GCAAGTGGCT GACCTTTCTG CCCGGCTACA TGAGATGAGT 
AATAGGACAA ATATCCCTGG TGGCCCTCCC CTCGCCCCTC TGGGTCCTGG TCTTCCACCT 
CCTCCTCCAC CTCCGGGTCA GGCTGGTCTT CCTCCTCCAC CTCCTCCAGG AGGAGCACCA 
CCACCACCTC CGCCTCCCCC CGGTGGACCT CCTCCTCCTC CAGGCCTGCT TGGCTTCGGT 
GCTCCTCCTC CTCCTGGAGG TCTCTTGGGC TCCACACTGA AGAAGAAGAA CATCCCGCAA 
CCATCCAACC CGCTCAAGTC TTTTAACTGG ACCAAACTCA GTGAAAATAA GCTGGAAGGG 
ACAGTGTGGC TGGATCTGGA CGACGTCAGA GTCTTCAAAC AATTAGATCT GGAGGATATT 
GAGAAAACAT TCTCTGCTTA TCAGCGACAG CAGGACTTTC TGTTCAATAA CTTTAGACAG 
AAAGAGTCCG AGGACGACAC TGTCACTTCT AAAAAGGTCA AAGAACTGTC TGTCATCGAT 
GGACGACGTG CGCAAAACTG CAACATCCTT CTCTCCAAGT TGAAACTGTC CAACGAGGAG 
ATCAAGAGAG CCATATTGAC CATGGATGAG CAGGAGGATC TGCCCAAAGA CATGCTGGAG 
CAGCTGTTGA AGTTTGTGCC AGAAAAAAGT GATGTAGACC TTCTGGAGGA GCACAAACAT 
GAGCTGGAGC GAATGGCGAA GGCAGACCGC TTCCTCTACG AGATGAGCAG AATCAACCAC 
TACCAGCAAA GACTGCAGTC TCTTTACTTT AAAAAGAAGT TTGCAGAAAG AATAGCAGAA 
ATCAAGCCCA AAGTGGAAGC TCTCAGTAAA GCATCTAAAG AGGTTCTTCA GAGCAAGAAT 
CTCCGTCAGC TTCTGGAGAT TGTCCTGGCC TTCGGAAACT ACATGAACAA AGGCCAGAGA 
GGCAATGCTT ACGGCTTCAA AGTGTCCTCT CTCAACAAGA TCGCTGACAC CAAATCCAGC 
ATCGACAAAA ACGTCACTCT TCTGCATTAC CTGATTACCG TTCTTGAGCA GAAATACCCC 
AAAGTCAGTC TGATTCACGA GGATCTGCAG AACGTTCCAG TTGCGGCCAA AGTCAACATG 
ACCGAACTGG AGAAAGACAT TAACAATCTG CGCTCTGGCT TGAAAAGCGT GGAGACGGAG 
CTGGAGTACC AGAGGACTCA ACCTCAGACC TATGGAGACA AGTTTGTGTC TGTAGTGAGT 
CAGTTCATCA CTGTGGCTGG ATTCAGTTTC TCTGACATTG AGGACTCTCT TCAGGATGCT 
AAGGATTCGT TTGGGAAGGC CGTGCAGCAC TTCGGTGAGG ACGCCACACG CATGCAGCCT 
GATGAGTTCT TCGGCATCTT CGATCAGTTC CTGCAGGGCT TCAGTGAAGC TAAACAAGAC 
AATGAGAACA TGAGACGACG GAAAGAGGAG GAGGAGCGCA GGGCCCGTAT GGAGGCACAG 
CTCAAGGAGC AGCGCGAGAG GGAAAGAAAA TCCCGGAAGG CTAAAGAAAA CTGCGAGGAG 
GACGGAGAGT TCGACGACCT GGTGTCTGCA CTGCGATCTG GGGAGGTGTT TGACAAAGAT 
ATGTCAAAGA TGAAACACAA CCGTAAACGT CCCGTCAAAT CGAGTGCAGA AAGCAGCAGA 
GAAAGACCCG TCACCAAACT CAACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207260.1
CDS	145..3354
Translation	MMAPRKRGGRGLSFLCCCLKGSDHPEITYSLRHGSNFTLQTMEPTLPMPPTEELDAMFTELVDELDLTEKHRAAMFALPAEKKWQIYCSKKKEQEENKGATSWPEFYIDQINSMAARKSLLALEKEDEEERNKTIESLKTALRTQPMRFVTRFIDQDGLTCILNFLKTMDYETTESQIHTSLIGCIKALMNNSQGRAHVLSHSESINIIAQSLATENIKTKVAVLEIMGAVCLVPGGHKKILEAMLHYQKFACERTRFQTLLNDLDRSTGRYRDEVNLKTAIMSFINAVLSQGAGETSLEFRIHLRYEFLMLGIQPVIDKLRSHENSTLDRHLDFFEMLRNEDELALAKRFENVHVDTKSATQMFELIRKRINHTDAFPHFISVLQHCLHMPYKKTGNTVQYWVLLDRIVQQIILQNDKGLDPDVAPLENFDVKNVVRMLVNENEVKQWKEQAEKMRKEHNELQQKLEKKERECDAKAQEKEEMMQTLNKMKEKLEKESSEHKLVKQQVADLSARLHEMSNRTNIPGGPPLAPLGPGLPPPPPPPGQAGLPPPPPPGGAPPPPPPPPGGPPPPPGLLGFGAPPPPGGLLGSTLKKKNIPQPSNPLKSFNWTKLSENKLEGTVWLDLDDVRVFKQLDLEDIEKTFSAYQRQQDFLFNNFRQKESEDDTVTSKKVKELSVIDGRRAQNCNILLSKLKLSNEEIKRAILTMDEQEDLPKDMLEQLLKFVPEKSDVDLLEEHKHELERMAKADRFLYEMSRINHYQQRLQSLYFKKKFAERIAEIKPKVEALSKASKEVLQSKNLRQLLEIVLAFGNYMNKGQRGNAYGFKVSSLNKIADTKSSIDKNVTLLHYLITVLEQKYPKVSLIHEDLQNVPVAAKVNMTELEKDINNLRSGLKSVETELEYQRTQPQTYGDKFVSVVSQFITVAGFSFSDIEDSLQDAKDSFGKAVQHFGEDATRMQPDEFFGIFDQFLQGFSEAKQDNENMRRRKEEEERRARMEAQLKEQRERERKSRKAKENCEEDGEFDDLVSALRSGEVFDKDMSKMKHNRKRPVKSSAESSRERPVTKLNF

Target ORF information:

RefSeq Version	XM_017351771.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio dishevelled associated activator of morphogenesis 1a (daam1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351771.1

ORF Insert Sequence:

ATGATGGCCC CACGTAAGCG AGGAGGTCGG GGCCTGTCAT TCCTCTGCTG CTGTCTAAAA 
GGGAGCGATC ATCCCGAAAT CACCTACAGC CTCCGTCATG GCAGCAACTT TACACTGCAG 
ACCATGGAGC CCACCCTGCC AATGCCCCCC ACTGAGGAGC TGGATGCTAT GTTCACTGAA 
CTGGTGGATG AGCTGGATCT CACAGAGAAA CATAGAGCGG CCATGTTTGC CCTGCCTGCC 
GAGAAGAAAT GGCAAATCTA CTGTAGCAAG AAAAAGGAAC AGGAAGAGAA TAAAGGGGCG 
ACCAGCTGGC CTGAATTCTA CATCGATCAG ATCAATTCTA TGGCTGCTAG GAAGTCTTTG 
CTAGCTCTGG AGAAGGAGGA TGAGGAGGAG AGGAATAAAA CCATAGAGAG CCTGAAAACT 
GCTCTCCGCA CACAGCCCAT GAGGTTCGTG ACGCGCTTCA TAGACCAGGA TGGCCTGACG 
TGCATCTTAA ATTTTCTGAA AACGATGGAC TATGAGACGA CCGAATCGCA GATCCACACG 
TCGCTCATCG GCTGCATTAA AGCCCTGATG AACAACTCTC AGGGCCGAGC GCACGTGCTC 
TCGCATTCAG AAAGCATCAA CATCATTGCT CAAAGCCTGG CCACCGAGAA CATCAAGACT 
AAAGTGGCTG TTCTGGAGAT CATGGGTGCT GTTTGTTTGG TTCCTGGAGG ACACAAGAAA 
ATTCTGGAAG CCATGCTGCA TTATCAGAAG TTTGCCTGCG AGCGGACGCG CTTTCAGACT 
CTCCTGAATG ACTTGGACAG GAGTACGGGC CGCTACAGAG ATGAGGTGAA CCTCAAGACG 
GCCATCATGT CCTTCATCAA CGCCGTCCTC AGTCAAGGAG CAGGGGAGAC CAGTTTGGAG 
TTCAGAATTC ATTTGCGGTA TGAGTTTCTA ATGCTGGGCA TCCAACCAGT GATTGATAAA 
TTACGGTCCC ATGAAAACTC CACGTTAGAT CGGCATTTGG ACTTCTTTGA GATGCTACGA 
AATGAAGATG AATTAGCTCT CGCTAAGCGG TTTGAAAACG TTCATGTAGA TACTAAAAGT 
GCCACTCAGA TGTTCGAGCT CATCCGGAAG AGGATAAACC ACACAGACGC CTTCCCGCAC 
TTTATATCCG TCTTACAGCA CTGTCTTCAT ATGCCTTACA AGAAAACTGG GAACACTGTG 
CAGTACTGGG TTCTCCTAGA CAGAATTGTC CAGCAGATCA TCCTGCAGAA CGACAAGGGG 
CTCGACCCAG ATGTGGCACC GCTGGAGAAC TTTGATGTCA AAAACGTGGT GCGAATGCTG 
GTCAATGAGA ACGAGGTGAA ACAATGGAAA GAGCAAGCGG AAAAAATGAG GAAAGAACAT 
AATGAACTTC AGCAGAAGCT GGAAAAGAAA GAAAGGGAGT GTGATGCTAA AGCCCAAGAG 
AAGGAAGAAA TGATGCAAAC GCTCAATAAG ATGAAAGAGA AGCTGGAAAA GGAAAGCAGT 
GAACATAAGC TTGTTAAGCA GCAAGTGGCT GACCTTTCTG CCCGGCTACA TGAGATGAGT 
AATAGGACAA ATATCCCTGG TGGCCCTCCC CTCGCCCCTC TGGGTCCTGG TCTTCCACCT 
CCTCCTCCAC CTCCGGGTCA GGCTGGTCTT CCTCCTCCAC CTCCTCCAGG AGGAGCACCA 
CCACCACCTC CGCCTCCCCC CGGTGGACCT CCTCCTCCTC CAGGCCTGCT TGGCTTCGGT 
GCTCCTCCTC CTCCTGGAGG TCTCTTGGGC TCCACACTGA AGAAGAAGAA CATCCCGCAA 
CCATCCAACC CGCTCAAGTC TTTTAACTGG ACCAAACTCA GTGAAAATAA GCTGGAAGGG 
ACAGTGTGGC TGGATCTGGA CGACGTCAGA GTCTTCAAAC AATTAGATCT GGAGGATATT 
GAGAAAACAT TCTCTGCTTA TCAGCGACAG CAGGACTTTC TGTTCAATAA CTTTAGACAG 
AAAGAGTCCG AGGACGACAC TGTCACTTCT AAAAAGGTCA AAGAACTGTC TGTCATCGAT 
GGACGACGTG CGCAAAACTG CAACATCCTT CTCTCCAAGT TGAAACTGTC CAACGAGGAG 
ATCAAGAGAG CCATATTGAC CATGGATGAG CAGGAGGATC TGCCCAAAGA CATGCTGGAG 
CAGCTGTTGA AGTTTGTGCC AGAAAAAAGT GATGTAGACC TTCTGGAGGA GCACAAACAT 
GAGCTGGAGC GAATGGCGAA GGCAGACCGC TTCCTCTACG AGATGAGCAG AATCAACCAC 
TACCAGCAAA GACTGCAGTC TCTTTACTTT AAAAAGAAGT TTGCAGAAAG AATAGCAGAA 
ATCAAGCCCA AAGTGGAAGC TCTCAGTAAA GCATCTAAAG AGGTTCTTCA GAGCAAGAAT 
CTCCGTCAGC TTCTGGAGAT TGTCCTGGCC TTCGGAAACT ACATGAACAA AGGCCAGAGA 
GGCAATGCTT ACGGCTTCAA AGTGTCCTCT CTCAACAAGA TCGCTGACAC CAAATCCAGC 
ATCGACAAAA ACGTCACTCT TCTGCATTAC CTGATTACCG TTCTTGAGCA GAAATACCCC 
AAAGTCAGTC TGATTCACGA GGATCTGCAG AACGTTCCAG TTGCGGCCAA AGTCAACATG 
ACCGAACTGG AGAAAGACAT TAACAATCTG CGCTCTGGCT TGAAAAGCGT GGAGACGGAG 
CTGGAGTACC AGAGGACTCA ACCTCAGACC TATGGAGACA AGTTTGTGTC TGTAGTGAGT 
CAGTTCATCA CTGTGGCTGG ATTCAGTTTC TCTGACATTG AGGACTCTCT TCAGGATGCT 
AAGGATTCGT TTGGGAAGGC CGTGCAGCAC TTCGGTGAGG ACGCCACACG CATGCAGCCT 
GATGAGTTCT TCGGCATCTT CGATCAGTTC CTGCAGGGCT TCAGTGAAGC TAAACAAGAC 
AATGAGAACA TGAGACGACG GAAAGAGGAG GAGGAGCGCA GGGCCCGTAT GGAGGCACAG 
CTCAAGGAGC AGCGCGAGAG GGAAAGAAAA TCCCGGAAGG CTAAAGAAAA CTGCGAGGAG 
GACGGAGAGT TCGACGACCT GGTGTCTGCA CTGCGATCTG GGGAGGTGTT TGACAAAGAT 
ATGTCAAAGA TGAAACACAA CCGTAAACGT CCCGTCAAAT CGAGTGCAGA AAGCAGCAGA 
GAAAGACCCG TCACCAAACT CAACTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAAM1A ( XM_017351772.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017207261.1 Danio rerio dishevelled associated activator of morphogenesis 1a (daam1a), transcript variant X3, mRNA.

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CloneID	ODa22321
Clone ID Related Accession (Same CDS sequence)	XM_017351772.1 , XM_017351772.2
Accession Version	XM_017351772.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	disheveled-associated activator of morphogenesis 1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCC CACGTAAGCG AGGAGGTCGG GGCCTGTCAT TCCTCTGCTG CTGTCTAAAA 
GGGAGCGATC ATCCCGAAAT CACCTACAGC CTCCGTCATG GCAGCAACTT TACACTGCAG 
ACCATGGAGC CCACCCTGCC AATGCCCCCC ACTGAGGAGC TGGATGCTAT GTTCACTGAA 
CTGGTGGATG AGCTGGATCT CACAGAGAAA CATAGAGCGG CCATGTTTGC CCTGCCTGCC 
GAGAAGAAAT GGCAAATCTA CTGTAGCAAG AAAAAGGAAC AGGAAGAGAA TAAAGGGGCG 
ACCAGCTGGC CTGAATTCTA CATCGATCAG ATCAATTCTA TGGCTGCTAG GAAGTCTTTG 
CTAGCTCTGG AGAAGGAGGA TGAGGAGGAG AGGAATAAAA CCATAGAGAG CCTGAAAACT 
GCTCTCCGCA CACAGCCCAT GAGGTTCGTG ACGCGCTTCA TAGACCAGGA TGGCCTGACG 
TGCATCTTAA ATTTTCTGAA AACGATGGAC TATGAGACGA CCGAATCGCA GATCCACACG 
TCGCTCATCG GCTGCATTAA AGCCCTGATG AACAACTCTC AGGGCCGAGC GCACGTGCTC 
TCGCATTCAG AAAGCATCAA CATCATTGCT CAAAGCCTGG CCACCGAGAA CATCAAGACT 
AAAGTGGCTG TTCTGGAGAT CATGGGTGCT GTTTGTTTGG TTCCTGGAGG ACACAAGAAA 
ATTCTGGAAG CCATGCTGCA TTATCAGAAG TTTGCCTGCG AGCGGACGCG CTTTCAGACT 
CTCCTGAATG ACTTGGACAG GAGTACGGGC CGCTACAGAG ATGAGGTGAA CCTCAAGACG 
GCCATCATGT CCTTCATCAA CGCCGTCCTC AGTCAAGGAG CAGGGGAGAC CAGTTTGGAG 
TTCAGAATTC ATTTGCGGTA TGAGTTTCTA ATGCTGGGCA TCCAACCAGT GATTGATAAA 
TTACGGTCCC ATGAAAACTC CACGTTAGAT CGGCATTTGG ACTTCTTTGA GATGCTACGA 
AATGAAGATG AATTAGCTCT CGCTAAGCGG TTTGAAAACG TTCATGTAGA TACTAAAAGT 
GCCACTCAGA TGTTCGAGCT CATCCGGAAG AGGATAAACC ACACAGACGC CTTCCCGCAC 
TTTATATCCG TCTTACAGCA CTGTCTTCAT ATGCCTTACA AGAAAACTGG GAACACTGTG 
CAGTACTGGG TTCTCCTAGA CAGAATTGTC CAGCAGATCA TCCTGCAGAA CGACAAGGGG 
CTCGACCCAG ATGTGGCACC GCTGGAGAAC TTTGATGTCA AAAACGTGGT GCGAATGCTG 
GTCAATGAGA ACGAGGTGAA ACAATGGAAA GAGCAAGCGG AAAAAATGAG GAAAGAACAT 
AATGAACTTC AGCAGAAGCT GGAAAAGAAA GAAAGGGAGT GTGATGCTAA AGCCCAAGAG 
AAGGAAGAAA TGATGCAAAC GCTCAATAAG ATGAAAGAGA AGCTGGAAAA GGAAAGCAGT 
GAACATAAGC TTGTTAAGCA GCAAGTGGCT GACCTTTCTG CCCGGCTACA TGAGATGAGT 
AATAGGACAA ATATCCCTGG TGGCCCTCCC CTCGCCCCTC TGGGTCCTGG TCTTCCACCT 
CCTCCTCCAC CTCCGGGTCA GGCTGGTCTT CCTCCTCCAC CTCCTCCAGG AGGAGCACCA 
CCACCACCTC CGCCTCCCCC CGGTGGACCT CCTCCTCCTC CAGGCCTGCT TGGCTTCGGT 
GCTCCTCCTC CTCCTGGAGG TCTCTTGGGC TCCACACTGA AGAAGAAGAA CATCCCGCAA 
CCATCCAACC CGCTCAAGTC TTTTAACTGG ACCAAACTCA GTGAAAATAA GCTGGAAGGG 
ACAGTGTGGC TGGATCTGGA CGACGTCAGA GTCTTCAAAC AATTAGATCT GGAGGATATT 
GAGAAAACAT TCTCTGCTTA TCAGCGACAG CAGAAAGAGT CCGAGGACGA CACTGTCACT 
TCTAAAAAGG TCAAAGAACT GTCTGTCATC GATGGACGAC GTGCGCAAAA CTGCAACATC 
CTTCTCTCCA AGTTGAAACT GTCCAACGAG GAGATCAAGA GAGCCATATT GACCATGGAT 
GAGCAGGAGG ATCTGCCCAA AGACATGCTG GAGCAGCTGT TGAAGTTTGT GCCAGAAAAA 
AGTGATGTAG ACCTTCTGGA GGAGCACAAA CATGAGCTGG AGCGAATGGC GAAGGCAGAC 
CGCTTCCTCT ACGAGATGAG CAGAATCAAC CACTACCAGC AAAGACTGCA GTCTCTTTAC 
TTTAAAAAGA AGTTTGCAGA AAGAATAGCA GAAATCAAGC CCAAAGTGGA AGCTCTCAGT 
AAAGCATCTA AAGAGGTTCT TCAGAGCAAG AATCTCCGTC AGCTTCTGGA GATTGTCCTG 
GCCTTCGGAA ACTACATGAA CAAAGGCCAG AGAGGCAATG CTTACGGCTT CAAAGTGTCC 
TCTCTCAACA AGATCGCTGA CACCAAATCC AGCATCGACA AAAACGTCAC TCTTCTGCAT 
TACCTGATTA CCGTTCTTGA GCAGAAATAC CCCAAAGTCA GTCTGATTCA CGAGGATCTG 
CAGAACGTTC CAGTTGCGGC CAAAGTCAAC ATGACCGAAC TGGAGAAAGA CATTAACAAT 
CTGCGCTCTG GCTTGAAAAG CGTGGAGACG GAGCTGGAGT ACCAGAGGAC TCAACCTCAG 
ACCTATGGAG ACAAGTTTGT GTCTGTAGTG AGTCAGTTCA TCACTGTGGC TGGATTCAGT 
TTCTCTGACA TTGAGGACTC TCTTCAGGAT GCTAAGGATT CGTTTGGGAA GGCCGTGCAG 
CACTTCGGTG AGGACGCCAC ACGCATGCAG CCTGATGAGT TCTTCGGCAT CTTCGATCAG 
TTCCTGCAGG GCTTCAGTGA AGCTAAACAA GACAATGAGA ACATGAGACG ACGGAAAGAG 
GAGGAGGAGC GCAGGGCCCG TATGGAGGCA CAGCTCAAGG AGCAGCGCGA GAGGGAAAGA 
AAATCCCGGA AGGCTAAAGA AAACTGCGAG GAGGACGGAG AGTTCGACGA CCTGGTGTCT 
GCACTGCGAT CTGGGGAGGT GTTTGACAAA GATATGTCAA AGATGAAACA CAACCGTAAA 
CGTCCCGTCA AATCGAGTGC AGAAAGCAGC AGAGAAAGAC CCGTCACCAA ACTCAACTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207261.1
CDS	145..3327
Translation	MMAPRKRGGRGLSFLCCCLKGSDHPEITYSLRHGSNFTLQTMEPTLPMPPTEELDAMFTELVDELDLTEKHRAAMFALPAEKKWQIYCSKKKEQEENKGATSWPEFYIDQINSMAARKSLLALEKEDEEERNKTIESLKTALRTQPMRFVTRFIDQDGLTCILNFLKTMDYETTESQIHTSLIGCIKALMNNSQGRAHVLSHSESINIIAQSLATENIKTKVAVLEIMGAVCLVPGGHKKILEAMLHYQKFACERTRFQTLLNDLDRSTGRYRDEVNLKTAIMSFINAVLSQGAGETSLEFRIHLRYEFLMLGIQPVIDKLRSHENSTLDRHLDFFEMLRNEDELALAKRFENVHVDTKSATQMFELIRKRINHTDAFPHFISVLQHCLHMPYKKTGNTVQYWVLLDRIVQQIILQNDKGLDPDVAPLENFDVKNVVRMLVNENEVKQWKEQAEKMRKEHNELQQKLEKKERECDAKAQEKEEMMQTLNKMKEKLEKESSEHKLVKQQVADLSARLHEMSNRTNIPGGPPLAPLGPGLPPPPPPPGQAGLPPPPPPGGAPPPPPPPPGGPPPPPGLLGFGAPPPPGGLLGSTLKKKNIPQPSNPLKSFNWTKLSENKLEGTVWLDLDDVRVFKQLDLEDIEKTFSAYQRQQKESEDDTVTSKKVKELSVIDGRRAQNCNILLSKLKLSNEEIKRAILTMDEQEDLPKDMLEQLLKFVPEKSDVDLLEEHKHELERMAKADRFLYEMSRINHYQQRLQSLYFKKKFAERIAEIKPKVEALSKASKEVLQSKNLRQLLEIVLAFGNYMNKGQRGNAYGFKVSSLNKIADTKSSIDKNVTLLHYLITVLEQKYPKVSLIHEDLQNVPVAAKVNMTELEKDINNLRSGLKSVETELEYQRTQPQTYGDKFVSVVSQFITVAGFSFSDIEDSLQDAKDSFGKAVQHFGEDATRMQPDEFFGIFDQFLQGFSEAKQDNENMRRRKEEEERRARMEAQLKEQRERERKSRKAKENCEEDGEFDDLVSALRSGEVFDKDMSKMKHNRKRPVKSSAESSRERPVTKLNF

Target ORF information:

RefSeq Version	XM_017351772.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio dishevelled associated activator of morphogenesis 1a (daam1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351772.1

ORF Insert Sequence:

ATGATGGCCC CACGTAAGCG AGGAGGTCGG GGCCTGTCAT TCCTCTGCTG CTGTCTAAAA 
GGGAGCGATC ATCCCGAAAT CACCTACAGC CTCCGTCATG GCAGCAACTT TACACTGCAG 
ACCATGGAGC CCACCCTGCC AATGCCCCCC ACTGAGGAGC TGGATGCTAT GTTCACTGAA 
CTGGTGGATG AGCTGGATCT CACAGAGAAA CATAGAGCGG CCATGTTTGC CCTGCCTGCC 
GAGAAGAAAT GGCAAATCTA CTGTAGCAAG AAAAAGGAAC AGGAAGAGAA TAAAGGGGCG 
ACCAGCTGGC CTGAATTCTA CATCGATCAG ATCAATTCTA TGGCTGCTAG GAAGTCTTTG 
CTAGCTCTGG AGAAGGAGGA TGAGGAGGAG AGGAATAAAA CCATAGAGAG CCTGAAAACT 
GCTCTCCGCA CACAGCCCAT GAGGTTCGTG ACGCGCTTCA TAGACCAGGA TGGCCTGACG 
TGCATCTTAA ATTTTCTGAA AACGATGGAC TATGAGACGA CCGAATCGCA GATCCACACG 
TCGCTCATCG GCTGCATTAA AGCCCTGATG AACAACTCTC AGGGCCGAGC GCACGTGCTC 
TCGCATTCAG AAAGCATCAA CATCATTGCT CAAAGCCTGG CCACCGAGAA CATCAAGACT 
AAAGTGGCTG TTCTGGAGAT CATGGGTGCT GTTTGTTTGG TTCCTGGAGG ACACAAGAAA 
ATTCTGGAAG CCATGCTGCA TTATCAGAAG TTTGCCTGCG AGCGGACGCG CTTTCAGACT 
CTCCTGAATG ACTTGGACAG GAGTACGGGC CGCTACAGAG ATGAGGTGAA CCTCAAGACG 
GCCATCATGT CCTTCATCAA CGCCGTCCTC AGTCAAGGAG CAGGGGAGAC CAGTTTGGAG 
TTCAGAATTC ATTTGCGGTA TGAGTTTCTA ATGCTGGGCA TCCAACCAGT GATTGATAAA 
TTACGGTCCC ATGAAAACTC CACGTTAGAT CGGCATTTGG ACTTCTTTGA GATGCTACGA 
AATGAAGATG AATTAGCTCT CGCTAAGCGG TTTGAAAACG TTCATGTAGA TACTAAAAGT 
GCCACTCAGA TGTTCGAGCT CATCCGGAAG AGGATAAACC ACACAGACGC CTTCCCGCAC 
TTTATATCCG TCTTACAGCA CTGTCTTCAT ATGCCTTACA AGAAAACTGG GAACACTGTG 
CAGTACTGGG TTCTCCTAGA CAGAATTGTC CAGCAGATCA TCCTGCAGAA CGACAAGGGG 
CTCGACCCAG ATGTGGCACC GCTGGAGAAC TTTGATGTCA AAAACGTGGT GCGAATGCTG 
GTCAATGAGA ACGAGGTGAA ACAATGGAAA GAGCAAGCGG AAAAAATGAG GAAAGAACAT 
AATGAACTTC AGCAGAAGCT GGAAAAGAAA GAAAGGGAGT GTGATGCTAA AGCCCAAGAG 
AAGGAAGAAA TGATGCAAAC GCTCAATAAG ATGAAAGAGA AGCTGGAAAA GGAAAGCAGT 
GAACATAAGC TTGTTAAGCA GCAAGTGGCT GACCTTTCTG CCCGGCTACA TGAGATGAGT 
AATAGGACAA ATATCCCTGG TGGCCCTCCC CTCGCCCCTC TGGGTCCTGG TCTTCCACCT 
CCTCCTCCAC CTCCGGGTCA GGCTGGTCTT CCTCCTCCAC CTCCTCCAGG AGGAGCACCA 
CCACCACCTC CGCCTCCCCC CGGTGGACCT CCTCCTCCTC CAGGCCTGCT TGGCTTCGGT 
GCTCCTCCTC CTCCTGGAGG TCTCTTGGGC TCCACACTGA AGAAGAAGAA CATCCCGCAA 
CCATCCAACC CGCTCAAGTC TTTTAACTGG ACCAAACTCA GTGAAAATAA GCTGGAAGGG 
ACAGTGTGGC TGGATCTGGA CGACGTCAGA GTCTTCAAAC AATTAGATCT GGAGGATATT 
GAGAAAACAT TCTCTGCTTA TCAGCGACAG CAGAAAGAGT CCGAGGACGA CACTGTCACT 
TCTAAAAAGG TCAAAGAACT GTCTGTCATC GATGGACGAC GTGCGCAAAA CTGCAACATC 
CTTCTCTCCA AGTTGAAACT GTCCAACGAG GAGATCAAGA GAGCCATATT GACCATGGAT 
GAGCAGGAGG ATCTGCCCAA AGACATGCTG GAGCAGCTGT TGAAGTTTGT GCCAGAAAAA 
AGTGATGTAG ACCTTCTGGA GGAGCACAAA CATGAGCTGG AGCGAATGGC GAAGGCAGAC 
CGCTTCCTCT ACGAGATGAG CAGAATCAAC CACTACCAGC AAAGACTGCA GTCTCTTTAC 
TTTAAAAAGA AGTTTGCAGA AAGAATAGCA GAAATCAAGC CCAAAGTGGA AGCTCTCAGT 
AAAGCATCTA AAGAGGTTCT TCAGAGCAAG AATCTCCGTC AGCTTCTGGA GATTGTCCTG 
GCCTTCGGAA ACTACATGAA CAAAGGCCAG AGAGGCAATG CTTACGGCTT CAAAGTGTCC 
TCTCTCAACA AGATCGCTGA CACCAAATCC AGCATCGACA AAAACGTCAC TCTTCTGCAT 
TACCTGATTA CCGTTCTTGA GCAGAAATAC CCCAAAGTCA GTCTGATTCA CGAGGATCTG 
CAGAACGTTC CAGTTGCGGC CAAAGTCAAC ATGACCGAAC TGGAGAAAGA CATTAACAAT 
CTGCGCTCTG GCTTGAAAAG CGTGGAGACG GAGCTGGAGT ACCAGAGGAC TCAACCTCAG 
ACCTATGGAG ACAAGTTTGT GTCTGTAGTG AGTCAGTTCA TCACTGTGGC TGGATTCAGT 
TTCTCTGACA TTGAGGACTC TCTTCAGGAT GCTAAGGATT CGTTTGGGAA GGCCGTGCAG 
CACTTCGGTG AGGACGCCAC ACGCATGCAG CCTGATGAGT TCTTCGGCAT CTTCGATCAG 
TTCCTGCAGG GCTTCAGTGA AGCTAAACAA GACAATGAGA ACATGAGACG ACGGAAAGAG 
GAGGAGGAGC GCAGGGCCCG TATGGAGGCA CAGCTCAAGG AGCAGCGCGA GAGGGAAAGA 
AAATCCCGGA AGGCTAAAGA AAACTGCGAG GAGGACGGAG AGTTCGACGA CCTGGTGTCT 
GCACTGCGAT CTGGGGAGGT GTTTGACAAA GATATGTCAA AGATGAAACA CAACCGTAAA 
CGTCCCGTCA AATCGAGTGC AGAAAGCAGC AGAGAAAGAC CCGTCACCAA ACTCAACTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

DAAM1A ( XM_017351772.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017207261.1 Danio rerio dishevelled associated activator of morphogenesis 1a (daam1a), transcript variant X3, mRNA.

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CloneID	ODa22321
Clone ID Related Accession (Same CDS sequence)	XM_017351772.1 , XM_017351772.2
Accession Version	XM_017351772.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3183bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	disheveled-associated activator of morphogenesis 1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007128.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017351772.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCCC CACGTAAGCG AGGAGGTCGG GGCCTGTCAT TCCTCTGCTG CTGTCTAAAA 
GGGAGCGATC ATCCCGAAAT CACCTACAGC CTCCGTCATG GCAGCAACTT TACACTGCAG 
ACCATGGAGC CCACCCTGCC AATGCCCCCC ACTGAGGAGC TGGATGCTAT GTTCACTGAA 
CTGGTGGATG AGCTGGATCT CACAGAGAAA CATAGAGCGG CCATGTTTGC CCTGCCTGCC 
GAGAAGAAAT GGCAAATCTA CTGTAGCAAG AAAAAGGAAC AGGAAGAGAA TAAAGGGGCG 
ACCAGCTGGC CTGAATTCTA CATCGATCAG ATCAATTCTA TGGCTGCTAG GAAGTCTTTG 
CTAGCTCTGG AGAAGGAGGA TGAGGAGGAG AGGAATAAAA CCATAGAGAG CCTGAAAACT 
GCTCTCCGCA CACAGCCCAT GAGGTTCGTG ACGCGCTTCA TAGACCAGGA TGGCCTGACG 
TGCATCTTAA ATTTTCTGAA AACGATGGAC TATGAGACGA CCGAATCGCA GATCCACACG 
TCGCTCATCG GCTGCATTAA AGCCCTGATG AACAACTCTC AGGGCCGAGC GCACGTGCTC 
TCGCATTCAG AAAGCATCAA CATCATTGCT CAAAGCCTGG CCACCGAGAA CATCAAGACT 
AAAGTGGCTG TTCTGGAGAT CATGGGTGCT GTTTGTTTGG TTCCTGGAGG ACACAAGAAA 
ATTCTGGAAG CCATGCTGCA TTATCAGAAG TTTGCCTGCG AGCGGACGCG CTTTCAGACT 
CTCCTGAATG ACTTGGACAG GAGTACGGGC CGCTACAGAG ATGAGGTGAA CCTCAAGACG 
GCCATCATGT CCTTCATCAA CGCCGTCCTC AGTCAAGGAG CAGGGGAGAC CAGTTTGGAG 
TTCAGAATTC ATTTGCGGTA TGAGTTTCTA ATGCTGGGCA TCCAACCAGT GATTGATAAA 
TTACGGTCCC ATGAAAACTC CACGTTAGAT CGGCATTTGG ACTTCTTTGA GATGCTACGA 
AATGAAGATG AATTAGCTCT CGCTAAGCGG TTTGAAAACG TTCATGTAGA TACTAAAAGT 
GCCACTCAGA TGTTCGAGCT CATCCGGAAG AGGATAAACC ACACAGACGC CTTCCCGCAC 
TTTATATCCG TCTTACAGCA CTGTCTTCAT ATGCCTTACA AGAAAACTGG GAACACTGTG 
CAGTACTGGG TTCTCCTAGA CAGAATTGTC CAGCAGATCA TCCTGCAGAA CGACAAGGGG 
CTCGACCCAG ATGTGGCACC GCTGGAGAAC TTTGATGTCA AAAACGTGGT GCGAATGCTG 
GTCAATGAGA ACGAGGTGAA ACAATGGAAA GAGCAAGCGG AAAAAATGAG GAAAGAACAT 
AATGAACTTC AGCAGAAGCT GGAAAAGAAA GAAAGGGAGT GTGATGCTAA AGCCCAAGAG 
AAGGAAGAAA TGATGCAAAC GCTCAATAAG ATGAAAGAGA AGCTGGAAAA GGAAAGCAGT 
GAACATAAGC TTGTTAAGCA GCAAGTGGCT GACCTTTCTG CCCGGCTACA TGAGATGAGT 
AATAGGACAA ATATCCCTGG TGGCCCTCCC CTCGCCCCTC TGGGTCCTGG TCTTCCACCT 
CCTCCTCCAC CTCCGGGTCA GGCTGGTCTT CCTCCTCCAC CTCCTCCAGG AGGAGCACCA 
CCACCACCTC CGCCTCCCCC CGGTGGACCT CCTCCTCCTC CAGGCCTGCT TGGCTTCGGT 
GCTCCTCCTC CTCCTGGAGG TCTCTTGGGC TCCACACTGA AGAAGAAGAA CATCCCGCAA 
CCATCCAACC CGCTCAAGTC TTTTAACTGG ACCAAACTCA GTGAAAATAA GCTGGAAGGG 
ACAGTGTGGC TGGATCTGGA CGACGTCAGA GTCTTCAAAC AATTAGATCT GGAGGATATT 
GAGAAAACAT TCTCTGCTTA TCAGCGACAG CAGAAAGAGT CCGAGGACGA CACTGTCACT 
TCTAAAAAGG TCAAAGAACT GTCTGTCATC GATGGACGAC GTGCGCAAAA CTGCAACATC 
CTTCTCTCCA AGTTGAAACT GTCCAACGAG GAGATCAAGA GAGCCATATT GACCATGGAT 
GAGCAGGAGG ATCTGCCCAA AGACATGCTG GAGCAGCTGT TGAAGTTTGT GCCAGAAAAA 
AGTGATGTAG ACCTTCTGGA GGAGCACAAA CATGAGCTGG AGCGAATGGC GAAGGCAGAC 
CGCTTCCTCT ACGAGATGAG CAGAATCAAC CACTACCAGC AAAGACTGCA GTCTCTTTAC 
TTTAAAAAGA AGTTTGCAGA AAGAATAGCA GAAATCAAGC CCAAAGTGGA AGCTCTCAGT 
AAAGCATCTA AAGAGGTTCT TCAGAGCAAG AATCTCCGTC AGCTTCTGGA GATTGTCCTG 
GCCTTCGGAA ACTACATGAA CAAAGGCCAG AGAGGCAATG CTTACGGCTT CAAAGTGTCC 
TCTCTCAACA AGATCGCTGA CACCAAATCC AGCATCGACA AAAACGTCAC TCTTCTGCAT 
TACCTGATTA CCGTTCTTGA GCAGAAATAC CCCAAAGTCA GTCTGATTCA CGAGGATCTG 
CAGAACGTTC CAGTTGCGGC CAAAGTCAAC ATGACCGAAC TGGAGAAAGA CATTAACAAT 
CTGCGCTCTG GCTTGAAAAG CGTGGAGACG GAGCTGGAGT ACCAGAGGAC TCAACCTCAG 
ACCTATGGAG ACAAGTTTGT GTCTGTAGTG AGTCAGTTCA TCACTGTGGC TGGATTCAGT 
TTCTCTGACA TTGAGGACTC TCTTCAGGAT GCTAAGGATT CGTTTGGGAA GGCCGTGCAG 
CACTTCGGTG AGGACGCCAC ACGCATGCAG CCTGATGAGT TCTTCGGCAT CTTCGATCAG 
TTCCTGCAGG GCTTCAGTGA AGCTAAACAA GACAATGAGA ACATGAGACG ACGGAAAGAG 
GAGGAGGAGC GCAGGGCCCG TATGGAGGCA CAGCTCAAGG AGCAGCGCGA GAGGGAAAGA 
AAATCCCGGA AGGCTAAAGA AAACTGCGAG GAGGACGGAG AGTTCGACGA CCTGGTGTCT 
GCACTGCGAT CTGGGGAGGT GTTTGACAAA GATATGTCAA AGATGAAACA CAACCGTAAA 
CGTCCCGTCA AATCGAGTGC AGAAAGCAGC AGAGAAAGAC CCGTCACCAA ACTCAACTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207261.1
CDS	166..3348
Translation	MMAPRKRGGRGLSFLCCCLKGSDHPEITYSLRHGSNFTLQTMEPTLPMPPTEELDAMFTELVDELDLTEKHRAAMFALPAEKKWQIYCSKKKEQEENKGATSWPEFYIDQINSMAARKSLLALEKEDEEERNKTIESLKTALRTQPMRFVTRFIDQDGLTCILNFLKTMDYETTESQIHTSLIGCIKALMNNSQGRAHVLSHSESINIIAQSLATENIKTKVAVLEIMGAVCLVPGGHKKILEAMLHYQKFACERTRFQTLLNDLDRSTGRYRDEVNLKTAIMSFINAVLSQGAGETSLEFRIHLRYEFLMLGIQPVIDKLRSHENSTLDRHLDFFEMLRNEDELALAKRFENVHVDTKSATQMFELIRKRINHTDAFPHFISVLQHCLHMPYKKTGNTVQYWVLLDRIVQQIILQNDKGLDPDVAPLENFDVKNVVRMLVNENEVKQWKEQAEKMRKEHNELQQKLEKKERECDAKAQEKEEMMQTLNKMKEKLEKESSEHKLVKQQVADLSARLHEMSNRTNIPGGPPLAPLGPGLPPPPPPPGQAGLPPPPPPGGAPPPPPPPPGGPPPPPGLLGFGAPPPPGGLLGSTLKKKNIPQPSNPLKSFNWTKLSENKLEGTVWLDLDDVRVFKQLDLEDIEKTFSAYQRQQKESEDDTVTSKKVKELSVIDGRRAQNCNILLSKLKLSNEEIKRAILTMDEQEDLPKDMLEQLLKFVPEKSDVDLLEEHKHELERMAKADRFLYEMSRINHYQQRLQSLYFKKKFAERIAEIKPKVEALSKASKEVLQSKNLRQLLEIVLAFGNYMNKGQRGNAYGFKVSSLNKIADTKSSIDKNVTLLHYLITVLEQKYPKVSLIHEDLQNVPVAAKVNMTELEKDINNLRSGLKSVETELEYQRTQPQTYGDKFVSVVSQFITVAGFSFSDIEDSLQDAKDSFGKAVQHFGEDATRMQPDEFFGIFDQFLQGFSEAKQDNENMRRRKEEEERRARMEAQLKEQRERERKSRKAKENCEEDGEFDDLVSALRSGEVFDKDMSKMKHNRKRPVKSSAESSRERPVTKLNF

Target ORF information:

RefSeq Version	XM_017351772.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio dishevelled associated activator of morphogenesis 1a (daam1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017351772.2

ORF Insert Sequence:

ATGATGGCCC CACGTAAGCG AGGAGGTCGG GGCCTGTCAT TCCTCTGCTG CTGTCTAAAA 
GGGAGCGATC ATCCCGAAAT CACCTACAGC CTCCGTCATG GCAGCAACTT TACACTGCAG 
ACCATGGAGC CCACCCTGCC AATGCCCCCC ACTGAGGAGC TGGATGCTAT GTTCACTGAA 
CTGGTGGATG AGCTGGATCT CACAGAGAAA CATAGAGCGG CCATGTTTGC CCTGCCTGCC 
GAGAAGAAAT GGCAAATCTA CTGTAGCAAG AAAAAGGAAC AGGAAGAGAA TAAAGGGGCG 
ACCAGCTGGC CTGAATTCTA CATCGATCAG ATCAATTCTA TGGCTGCTAG GAAGTCTTTG 
CTAGCTCTGG AGAAGGAGGA TGAGGAGGAG AGGAATAAAA CCATAGAGAG CCTGAAAACT 
GCTCTCCGCA CACAGCCCAT GAGGTTCGTG ACGCGCTTCA TAGACCAGGA TGGCCTGACG 
TGCATCTTAA ATTTTCTGAA AACGATGGAC TATGAGACGA CCGAATCGCA GATCCACACG 
TCGCTCATCG GCTGCATTAA AGCCCTGATG AACAACTCTC AGGGCCGAGC GCACGTGCTC 
TCGCATTCAG AAAGCATCAA CATCATTGCT CAAAGCCTGG CCACCGAGAA CATCAAGACT 
AAAGTGGCTG TTCTGGAGAT CATGGGTGCT GTTTGTTTGG TTCCTGGAGG ACACAAGAAA 
ATTCTGGAAG CCATGCTGCA TTATCAGAAG TTTGCCTGCG AGCGGACGCG CTTTCAGACT 
CTCCTGAATG ACTTGGACAG GAGTACGGGC CGCTACAGAG ATGAGGTGAA CCTCAAGACG 
GCCATCATGT CCTTCATCAA CGCCGTCCTC AGTCAAGGAG CAGGGGAGAC CAGTTTGGAG 
TTCAGAATTC ATTTGCGGTA TGAGTTTCTA ATGCTGGGCA TCCAACCAGT GATTGATAAA 
TTACGGTCCC ATGAAAACTC CACGTTAGAT CGGCATTTGG ACTTCTTTGA GATGCTACGA 
AATGAAGATG AATTAGCTCT CGCTAAGCGG TTTGAAAACG TTCATGTAGA TACTAAAAGT 
GCCACTCAGA TGTTCGAGCT CATCCGGAAG AGGATAAACC ACACAGACGC CTTCCCGCAC 
TTTATATCCG TCTTACAGCA CTGTCTTCAT ATGCCTTACA AGAAAACTGG GAACACTGTG 
CAGTACTGGG TTCTCCTAGA CAGAATTGTC CAGCAGATCA TCCTGCAGAA CGACAAGGGG 
CTCGACCCAG ATGTGGCACC GCTGGAGAAC TTTGATGTCA AAAACGTGGT GCGAATGCTG 
GTCAATGAGA ACGAGGTGAA ACAATGGAAA GAGCAAGCGG AAAAAATGAG GAAAGAACAT 
AATGAACTTC AGCAGAAGCT GGAAAAGAAA GAAAGGGAGT GTGATGCTAA AGCCCAAGAG 
AAGGAAGAAA TGATGCAAAC GCTCAATAAG ATGAAAGAGA AGCTGGAAAA GGAAAGCAGT 
GAACATAAGC TTGTTAAGCA GCAAGTGGCT GACCTTTCTG CCCGGCTACA TGAGATGAGT 
AATAGGACAA ATATCCCTGG TGGCCCTCCC CTCGCCCCTC TGGGTCCTGG TCTTCCACCT 
CCTCCTCCAC CTCCGGGTCA GGCTGGTCTT CCTCCTCCAC CTCCTCCAGG AGGAGCACCA 
CCACCACCTC CGCCTCCCCC CGGTGGACCT CCTCCTCCTC CAGGCCTGCT TGGCTTCGGT 
GCTCCTCCTC CTCCTGGAGG TCTCTTGGGC TCCACACTGA AGAAGAAGAA CATCCCGCAA 
CCATCCAACC CGCTCAAGTC TTTTAACTGG ACCAAACTCA GTGAAAATAA GCTGGAAGGG 
ACAGTGTGGC TGGATCTGGA CGACGTCAGA GTCTTCAAAC AATTAGATCT GGAGGATATT 
GAGAAAACAT TCTCTGCTTA TCAGCGACAG CAGAAAGAGT CCGAGGACGA CACTGTCACT 
TCTAAAAAGG TCAAAGAACT GTCTGTCATC GATGGACGAC GTGCGCAAAA CTGCAACATC 
CTTCTCTCCA AGTTGAAACT GTCCAACGAG GAGATCAAGA GAGCCATATT GACCATGGAT 
GAGCAGGAGG ATCTGCCCAA AGACATGCTG GAGCAGCTGT TGAAGTTTGT GCCAGAAAAA 
AGTGATGTAG ACCTTCTGGA GGAGCACAAA CATGAGCTGG AGCGAATGGC GAAGGCAGAC 
CGCTTCCTCT ACGAGATGAG CAGAATCAAC CACTACCAGC AAAGACTGCA GTCTCTTTAC 
TTTAAAAAGA AGTTTGCAGA AAGAATAGCA GAAATCAAGC CCAAAGTGGA AGCTCTCAGT 
AAAGCATCTA AAGAGGTTCT TCAGAGCAAG AATCTCCGTC AGCTTCTGGA GATTGTCCTG 
GCCTTCGGAA ACTACATGAA CAAAGGCCAG AGAGGCAATG CTTACGGCTT CAAAGTGTCC 
TCTCTCAACA AGATCGCTGA CACCAAATCC AGCATCGACA AAAACGTCAC TCTTCTGCAT 
TACCTGATTA CCGTTCTTGA GCAGAAATAC CCCAAAGTCA GTCTGATTCA CGAGGATCTG 
CAGAACGTTC CAGTTGCGGC CAAAGTCAAC ATGACCGAAC TGGAGAAAGA CATTAACAAT 
CTGCGCTCTG GCTTGAAAAG CGTGGAGACG GAGCTGGAGT ACCAGAGGAC TCAACCTCAG 
ACCTATGGAG ACAAGTTTGT GTCTGTAGTG AGTCAGTTCA TCACTGTGGC TGGATTCAGT 
TTCTCTGACA TTGAGGACTC TCTTCAGGAT GCTAAGGATT CGTTTGGGAA GGCCGTGCAG 
CACTTCGGTG AGGACGCCAC ACGCATGCAG CCTGATGAGT TCTTCGGCAT CTTCGATCAG 
TTCCTGCAGG GCTTCAGTGA AGCTAAACAA GACAATGAGA ACATGAGACG ACGGAAAGAG 
GAGGAGGAGC GCAGGGCCCG TATGGAGGCA CAGCTCAAGG AGCAGCGCGA GAGGGAAAGA 
AAATCCCGGA AGGCTAAAGA AAACTGCGAG GAGGACGGAG AGTTCGACGA CCTGGTGTCT 
GCACTGCGAT CTGGGGAGGT GTTTGACAAA GATATGTCAA AGATGAAACA CAACCGTAAA 
CGTCCCGTCA AATCGAGTGC AGAAAGCAGC AGAGAAAGAC CCGTCACCAA ACTCAACTTC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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DAAM1A cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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