LOC556989 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC556989
Entrez Gene ID	556989
Full Name	myosin-IIIb
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_679943.6	XP_685035.4	myosin-IIIb isoform X1
XM_005172588.3	XP_005172645.1	myosin-IIIb isoform X2
XM_005172588.4	XP_005172645.1	myosin-IIIb isoform X2
XM_679943.7	XP_685035.4	myosin-IIIb isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	MYO3AL	XP_424992.4
Danio rerio (zebrafish)	LOC556989	XP_005172645.1

Gene Ontology

The following LOC556989 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC556989 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa15679	XM_679943.7 Latest version!	Danio rerio myosin-IIIb (LOC556989), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa15680	XM_005172588.4 Latest version!	Danio rerio myosin-IIIb (LOC556989), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa15680	XM_005172588.3	Danio rerio myosin-IIIb (LOC556989), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa15679	XM_679943.6	Danio rerio myosin-IIIb (LOC556989), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC556989 ( XM_679943.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_685035.4 Danio rerio myosin-IIIb (LOC556989), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa15679
Clone ID Related Accession (Same CDS sequence)	XM_679943.6 , XM_679943.7
Accession Version	XM_679943.6	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	myosin-IIIb isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_679943.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTGA CCGAGCGTGG ATATAAACAC GAGCTGCTGA TTTGTAATGA AAGACAGTCA 
GATTTGAAGT TTGTAAGTTC ACGTAGATCA TTAGACAGAG AGATAATGGA GGAAAACCTG 
CCTGAAGATC TGGCGAATCT GTCTGAGCTG GACGAGAAAT GTTTGCTGGA GGTTTTGACT 
GGACGTTTCA CACAGAACCT CATCTATACG TATATAGGGG ACATCTTGGT GGCTATCAAT 
CCATTTAAAT ATCTTTCCAT CTATGAAAAA GAGGTATCCC AGCGTTATAA GTGCCATGAG 
AAGAAGTCTT TACCCCCGCA TATATTCGCT GTGGCGGACC GAGCCTATCA GTCCATGCTG 
GGACGTCTAG CTATGGGACC TAAGAATCAA TGCATCGTCA TCAGTGGTGA GAGCGGCGCG 
GGTAAAACGG AGAGCACCAA ACTGCTGCTG AGACACATCA TGGAGCTCTG CAAGGCCAAC 
TCCCAGCTGG AGCAGCAGAT CCTGCAGGTG AATCCGCTTC TGGAGGCCTT TGGGAATGCT 
CAGACGGTCA TGAATGACAA CAGCAGTCGC TTCGGGAAGT ACATCCAGCT CCGTTTCCAG 
AACTCCTCAG TTAAAGGAGC CAAAATCAAT GAGTACCTGC TGGAGAAATC TCGCGTGGTC 
CACCAGGACC AGGGGGAGCA GAACTTCCAC ATCTTCTACC TCATGTTGGC TGGAATTTCA 
TTGGAGGATA AGGAAATTTA CGGCCTTCTG GACCCGACGC TGTACCGGTA TTTGAATGGC 
AGGTTTGGGC AAGAAGACGT AGTGCAGACG TGGCGCGTGA AATATGCAGA GGTGTGTAAC 
GCCATGGACA TGGTAGGATT CGAAGAGCAG GAGAAAGTGG ACATGATGAC CATTCTCGCT 
GGCATTCTGT CTCTGGGAAA CATCACGTTC GAGACGACCG ACAGTGACGC TCTGAAAGTG 
TCTGAAGCGT CACGTGGATG GCTGAAAGCT ACTGCTGGTC AGTTTGGCGT CCAGGAAGAA 
GACCTGCTGA AGAGTCTGAC CTGCACCACG TCAATGACTC GAGGAGAAGC CATTCAAAGA 
CTCCACAACC AGCAGCAGGC CGAGGATGCC AGGGACTCCA TCGCTAAAGT GGCCTACGGT 
CGAGTGTTTG GGTGGATCGT CAGTAAAATC AACGAACAGC TCGCTCCAAA AGTAGACTTT 
GATGTGGAAC TCAACGAAAT AGGAATCCTC GACATCTTTG GCTTTGAAAA CTTTGCTGTA 
AACCGATTTG AGCAGCTCTG CATTAATCTG GCTAATGAAC AGCTCCAGTA TTTCTTCAAC 
CACCACATTT TTCTGATGGA GCAGAAGGAA TATAAAGAGG AAGGAATCAC GTGGGAGACC 
ATAACCTACA AGGACAACAA ACCCATACTG GACCTGTTTC TCATGAAGCC CATTGGGATT 
CTCTCACTGC TGGATGAACA AAGTGCCTTT CCTCAGGCCA CTGATCGAGA TTTTGTTGAC 
AAGTTAAACA GCAAGTTTAA ATCGGCTCCA AACTTCGAAG TGGTGCGAAA TCACACTCCT 
CTGTTCACTG TTGTGCACTA CGCTGGGAAG GTGCAATATA ATGCGAGTGG TTTTCTGGAG 
AAAAATAGAG ACACGATTCC AGCCAGCATT CGCGAGCTCT TCATTAACAG CGTCACTCCT 
CTCCTCAGCG TTTTATTCGC AGCCACAATC TCCAGAACGG GCACTCTAAT GCCAAGGAAA 
GCCAAGTTGC AAGTTTCAGA TGATAACTTT AACTCCACCA GGAAGCAGTC TGTTGGTGCA 
CAGTTCAAGC ATTCTCTGGC GGTTCTGATG GAGAAGATGT TTGCTGCCAG TCCTCACTTC 
GTCCGCTGCA TCAAACCCAA CCGCAGCAAG CAGCCCGATC AGCTGGACAG TAAACTGGTC 
CTGGATCAGC TGCGGTACAA TGGACTGATG GAAACGATCC GGATCCGCCG GGATGGTTTC 
TCGTGGCGTC CGACATTTAG GGAGTTTGCC GAGAGGTACA ACATTCTGCT GCTGAAACCG 
GATCTGCCCA CAGACAAGAG CAGTTGTATG TCAATCCTCA ACAGCACAGA ACTGTCAGGA 
TGGAAGTGCG GATCATCGCG GCTGTTTTTT AAGCTCTGCC ACCAGGTGGA GTTGGCCAAT 
TTGCTGGACA GACTGGGCAG AGCGGCGCTT GTCATTCAGA AAAATTTCCG AGCCCGGACC 
TGCCGCAGGA AGTATCTGTG TCTGCTGGCG GAGCTGCGGC GTCAGCGTCA GGCTCAGCGG 
GAGCGCGAGG AGCGTGAGGA GGAGGAGAGG AGACGCCAGG AGGAGGAGAA GAGACGGCAG 
CAGCAGCAGG AGGCGGAGAG GAAGAGGCGT CTGGAGGTGG AGCAGGAGAA CCTGAGACCA 
GCGTCTCCAC CGGTGCCTTT GCCCAGGAAG AAAAAGCCGG AGCCCAGGCC TCGCTCGGTC 
CTCAGCAGCA TCAGTAAAAC AGCAAGCGTG GCTCCAGTAC CACGACCCCG CAGCAAGCTT 
TTAGAGGCGA CTCCATTTGA CAACGATCTG AACGAGAACT TCCACCTGGA CGCGTCTCTG 
ATGCTGCAGC CAATCGATGA AGAGCGCATT AAAGAGAAGC ACCTGAAGAA GAGCAACACT 
ATTCGCTGGT TTAGAGAGAC GCAGGTGCGC AAGCTGACCC GGGATGGCTC TTTCCCACAC 
TGGTTCCACG GCATGATCAC ACGGAGGCAA GCGGAAGATC TTCTCATCGA CAAACCTCTA 
GGCTGCTTCT TGGTGCGAGT CGGTCAAAGT CGTGAGGGTT TCACTCTGAC ATTCAGAGGT 
GCCGATCGTT GTCGTCACTA CATGGTGGAG ATGCAGAGCA ATGGGAAGTA TGTGATCCTG 
GGTGAAGATC GAGCTCATTC TTCTCTCACA GACCTGGTCC AGTACCACAC TCAGGTGGGC 
ATCAAGCCCT TCATGGAGCT GCTGACGGTG CCATGTGGAC AGATGTGCGA TCAGGAGCCG 
GACTATGAGG AGCTCAAGGC TTTTTCCTCG GCGGTGGAGA GCGTTGACGG TCCCGCTGAA 
AACATGGACA CGGGGTCAAA TAAAGTGCAC TTGCAGATGC AGAGACTGTT CCTGCCACCA 
GGAGGAAACT CAAACCATGA GCACCACCGA GCCCCAGTGC TGAAGCGCAT CTCTGACCGC 
CGCAGACGAA TGGAGGCGGC ACAAACCGAA CGCAAGGAGG AGGATCTGAC CAATCAGAGC 
AGGCCTTTCC CTCGACTCTA CCCATCAATA CGGCTAGCCA TGAGGGAGAT CCAGCAAATC 
CAAGAGCAGA GCCAAGAACA GATCCAGATT CCCTCCAGCA AAGACCTGAG CTCCTGGAAG 
AGGACGGATT CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_685035.4
CDS	363..3737
Translation	MKVTERGYKHELLICNERQSDLKFVSSRRSLDREIMEENLPEDLANLSELDEKCLLEVLTGRFTQNLIYTYIGDILVAINPFKYLSIYEKEVSQRYKCHEKKSLPPHIFAVADRAYQSMLGRLAMGPKNQCIVISGESGAGKTESTKLLLRHIMELCKANSQLEQQILQVNPLLEAFGNAQTVMNDNSSRFGKYIQLRFQNSSVKGAKINEYLLEKSRVVHQDQGEQNFHIFYLMLAGISLEDKEIYGLLDPTLYRYLNGRFGQEDVVQTWRVKYAEVCNAMDMVGFEEQEKVDMMTILAGILSLGNITFETTDSDALKVSEASRGWLKATAGQFGVQEEDLLKSLTCTTSMTRGEAIQRLHNQQQAEDARDSIAKVAYGRVFGWIVSKINEQLAPKVDFDVELNEIGILDIFGFENFAVNRFEQLCINLANEQLQYFFNHHIFLMEQKEYKEEGITWETITYKDNKPILDLFLMKPIGILSLLDEQSAFPQATDRDFVDKLNSKFKSAPNFEVVRNHTPLFTVVHYAGKVQYNASGFLEKNRDTIPASIRELFINSVTPLLSVLFAATISRTGTLMPRKAKLQVSDDNFNSTRKQSVGAQFKHSLAVLMEKMFAASPHFVRCIKPNRSKQPDQLDSKLVLDQLRYNGLMETIRIRRDGFSWRPTFREFAERYNILLLKPDLPTDKSSCMSILNSTELSGWKCGSSRLFFKLCHQVELANLLDRLGRAALVIQKNFRARTCRRKYLCLLAELRRQRQAQREREEREEEERRRQEEEKRRQQQQEAERKRRLEVEQENLRPASPPVPLPRKKKPEPRPRSVLSSISKTASVAPVPRPRSKLLEATPFDNDLNENFHLDASLMLQPIDEERIKEKHLKKSNTIRWFRETQVRKLTRDGSFPHWFHGMITRRQAEDLLIDKPLGCFLVRVGQSREGFTLTFRGADRCRHYMVEMQSNGKYVILGEDRAHSSLTDLVQYHTQVGIKPFMELLTVPCGQMCDQEPDYEELKAFSSAVESVDGPAENMDTGSNKVHLQMQRLFLPPGGNSNHEHHRAPVLKRISDRRRRMEAAQTERKEEDLTNQSRPFPRLYPSIRLAMREIQQIQEQSQEQIQIPSSKDLSSWKRTDS

Target ORF information:

RefSeq Version	XM_679943.6
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myosin-IIIb (LOC556989), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_679943.6

ORF Insert Sequence:

ATGAAAGTGA CCGAGCGTGG ATATAAACAC GAGCTGCTGA TTTGTAATGA AAGACAGTCA 
GATTTGAAGT TTGTAAGTTC ACGTAGATCA TTAGACAGAG AGATAATGGA GGAAAACCTG 
CCTGAAGATC TGGCGAATCT GTCTGAGCTG GACGAGAAAT GTTTGCTGGA GGTTTTGACT 
GGACGTTTCA CACAGAACCT CATCTATACG TATATAGGGG ACATCTTGGT GGCTATCAAT 
CCATTTAAAT ATCTTTCCAT CTATGAAAAA GAGGTATCCC AGCGTTATAA GTGCCATGAG 
AAGAAGTCTT TACCCCCGCA TATATTCGCT GTGGCGGACC GAGCCTATCA GTCCATGCTG 
GGACGTCTAG CTATGGGACC TAAGAATCAA TGCATCGTCA TCAGTGGTGA GAGCGGCGCG 
GGTAAAACGG AGAGCACCAA ACTGCTGCTG AGACACATCA TGGAGCTCTG CAAGGCCAAC 
TCCCAGCTGG AGCAGCAGAT CCTGCAGGTG AATCCGCTTC TGGAGGCCTT TGGGAATGCT 
CAGACGGTCA TGAATGACAA CAGCAGTCGC TTCGGGAAGT ACATCCAGCT CCGTTTCCAG 
AACTCCTCAG TTAAAGGAGC CAAAATCAAT GAGTACCTGC TGGAGAAATC TCGCGTGGTC 
CACCAGGACC AGGGGGAGCA GAACTTCCAC ATCTTCTACC TCATGTTGGC TGGAATTTCA 
TTGGAGGATA AGGAAATTTA CGGCCTTCTG GACCCGACGC TGTACCGGTA TTTGAATGGC 
AGGTTTGGGC AAGAAGACGT AGTGCAGACG TGGCGCGTGA AATATGCAGA GGTGTGTAAC 
GCCATGGACA TGGTAGGATT CGAAGAGCAG GAGAAAGTGG ACATGATGAC CATTCTCGCT 
GGCATTCTGT CTCTGGGAAA CATCACGTTC GAGACGACCG ACAGTGACGC TCTGAAAGTG 
TCTGAAGCGT CACGTGGATG GCTGAAAGCT ACTGCTGGTC AGTTTGGCGT CCAGGAAGAA 
GACCTGCTGA AGAGTCTGAC CTGCACCACG TCAATGACTC GAGGAGAAGC CATTCAAAGA 
CTCCACAACC AGCAGCAGGC CGAGGATGCC AGGGACTCCA TCGCTAAAGT GGCCTACGGT 
CGAGTGTTTG GGTGGATCGT CAGTAAAATC AACGAACAGC TCGCTCCAAA AGTAGACTTT 
GATGTGGAAC TCAACGAAAT AGGAATCCTC GACATCTTTG GCTTTGAAAA CTTTGCTGTA 
AACCGATTTG AGCAGCTCTG CATTAATCTG GCTAATGAAC AGCTCCAGTA TTTCTTCAAC 
CACCACATTT TTCTGATGGA GCAGAAGGAA TATAAAGAGG AAGGAATCAC GTGGGAGACC 
ATAACCTACA AGGACAACAA ACCCATACTG GACCTGTTTC TCATGAAGCC CATTGGGATT 
CTCTCACTGC TGGATGAACA AAGTGCCTTT CCTCAGGCCA CTGATCGAGA TTTTGTTGAC 
AAGTTAAACA GCAAGTTTAA ATCGGCTCCA AACTTCGAAG TGGTGCGAAA TCACACTCCT 
CTGTTCACTG TTGTGCACTA CGCTGGGAAG GTGCAATATA ATGCGAGTGG TTTTCTGGAG 
AAAAATAGAG ACACGATTCC AGCCAGCATT CGCGAGCTCT TCATTAACAG CGTCACTCCT 
CTCCTCAGCG TTTTATTCGC AGCCACAATC TCCAGAACGG GCACTCTAAT GCCAAGGAAA 
GCCAAGTTGC AAGTTTCAGA TGATAACTTT AACTCCACCA GGAAGCAGTC TGTTGGTGCA 
CAGTTCAAGC ATTCTCTGGC GGTTCTGATG GAGAAGATGT TTGCTGCCAG TCCTCACTTC 
GTCCGCTGCA TCAAACCCAA CCGCAGCAAG CAGCCCGATC AGCTGGACAG TAAACTGGTC 
CTGGATCAGC TGCGGTACAA TGGACTGATG GAAACGATCC GGATCCGCCG GGATGGTTTC 
TCGTGGCGTC CGACATTTAG GGAGTTTGCC GAGAGGTACA ACATTCTGCT GCTGAAACCG 
GATCTGCCCA CAGACAAGAG CAGTTGTATG TCAATCCTCA ACAGCACAGA ACTGTCAGGA 
TGGAAGTGCG GATCATCGCG GCTGTTTTTT AAGCTCTGCC ACCAGGTGGA GTTGGCCAAT 
TTGCTGGACA GACTGGGCAG AGCGGCGCTT GTCATTCAGA AAAATTTCCG AGCCCGGACC 
TGCCGCAGGA AGTATCTGTG TCTGCTGGCG GAGCTGCGGC GTCAGCGTCA GGCTCAGCGG 
GAGCGCGAGG AGCGTGAGGA GGAGGAGAGG AGACGCCAGG AGGAGGAGAA GAGACGGCAG 
CAGCAGCAGG AGGCGGAGAG GAAGAGGCGT CTGGAGGTGG AGCAGGAGAA CCTGAGACCA 
GCGTCTCCAC CGGTGCCTTT GCCCAGGAAG AAAAAGCCGG AGCCCAGGCC TCGCTCGGTC 
CTCAGCAGCA TCAGTAAAAC AGCAAGCGTG GCTCCAGTAC CACGACCCCG CAGCAAGCTT 
TTAGAGGCGA CTCCATTTGA CAACGATCTG AACGAGAACT TCCACCTGGA CGCGTCTCTG 
ATGCTGCAGC CAATCGATGA AGAGCGCATT AAAGAGAAGC ACCTGAAGAA GAGCAACACT 
ATTCGCTGGT TTAGAGAGAC GCAGGTGCGC AAGCTGACCC GGGATGGCTC TTTCCCACAC 
TGGTTCCACG GCATGATCAC ACGGAGGCAA GCGGAAGATC TTCTCATCGA CAAACCTCTA 
GGCTGCTTCT TGGTGCGAGT CGGTCAAAGT CGTGAGGGTT TCACTCTGAC ATTCAGAGGT 
GCCGATCGTT GTCGTCACTA CATGGTGGAG ATGCAGAGCA ATGGGAAGTA TGTGATCCTG 
GGTGAAGATC GAGCTCATTC TTCTCTCACA GACCTGGTCC AGTACCACAC TCAGGTGGGC 
ATCAAGCCCT TCATGGAGCT GCTGACGGTG CCATGTGGAC AGATGTGCGA TCAGGAGCCG 
GACTATGAGG AGCTCAAGGC TTTTTCCTCG GCGGTGGAGA GCGTTGACGG TCCCGCTGAA 
AACATGGACA CGGGGTCAAA TAAAGTGCAC TTGCAGATGC AGAGACTGTT CCTGCCACCA 
GGAGGAAACT CAAACCATGA GCACCACCGA GCCCCAGTGC TGAAGCGCAT CTCTGACCGC 
CGCAGACGAA TGGAGGCGGC ACAAACCGAA CGCAAGGAGG AGGATCTGAC CAATCAGAGC 
AGGCCTTTCC CTCGACTCTA CCCATCAATA CGGCTAGCCA TGAGGGAGAT CCAGCAAATC 
CAAGAGCAGA GCCAAGAACA GATCCAGATT CCCTCCAGCA AAGACCTGAG CTCCTGGAAG 
AGGACGGATT CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC556989 ( XM_679943.7 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_685035.4 Danio rerio myosin-IIIb (LOC556989), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa15679
Clone ID Related Accession (Same CDS sequence)	XM_679943.6 , XM_679943.7
Accession Version	XM_679943.7 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3375bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	myosin-IIIb isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_679943.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTGA CCGAGCGTGG ATATAAACAC GAGCTGCTGA TTTGTAATGA AAGACAGTCA 
GATTTGAAGT TTGTAAGTTC ACGTAGATCA TTAGACAGAG AGATAATGGA GGAAAACCTG 
CCTGAAGATC TGGCGAATCT GTCTGAGCTG GACGAGAAAT GTTTGCTGGA GGTTTTGACT 
GGACGTTTCA CACAGAACCT CATCTATACG TATATAGGGG ACATCTTGGT GGCTATCAAT 
CCATTTAAAT ATCTTTCCAT CTATGAAAAA GAGGTATCCC AGCGTTATAA GTGCCATGAG 
AAGAAGTCTT TACCCCCGCA TATATTCGCT GTGGCGGACC GAGCCTATCA GTCCATGCTG 
GGACGTCTAG CTATGGGACC TAAGAATCAA TGCATCGTCA TCAGTGGTGA GAGCGGCGCG 
GGTAAAACGG AGAGCACCAA ACTGCTGCTG AGACACATCA TGGAGCTCTG CAAGGCCAAC 
TCCCAGCTGG AGCAGCAGAT CCTGCAGGTG AATCCGCTTC TGGAGGCCTT TGGGAATGCT 
CAGACGGTCA TGAATGACAA CAGCAGTCGC TTCGGGAAGT ACATCCAGCT CCGTTTCCAG 
AACTCCTCAG TTAAAGGAGC CAAAATCAAT GAGTACCTGC TGGAGAAATC TCGCGTGGTC 
CACCAGGACC AGGGGGAGCA GAACTTCCAC ATCTTCTACC TCATGTTGGC TGGAATTTCA 
TTGGAGGATA AGGAAATTTA CGGCCTTCTG GACCCGACGC TGTACCGGTA TTTGAATGGC 
AGGTTTGGGC AAGAAGACGT AGTGCAGACG TGGCGCGTGA AATATGCAGA GGTGTGTAAC 
GCCATGGACA TGGTAGGATT CGAAGAGCAG GAGAAAGTGG ACATGATGAC CATTCTCGCT 
GGCATTCTGT CTCTGGGAAA CATCACGTTC GAGACGACCG ACAGTGACGC TCTGAAAGTG 
TCTGAAGCGT CACGTGGATG GCTGAAAGCT ACTGCTGGTC AGTTTGGCGT CCAGGAAGAA 
GACCTGCTGA AGAGTCTGAC CTGCACCACG TCAATGACTC GAGGAGAAGC CATTCAAAGA 
CTCCACAACC AGCAGCAGGC CGAGGATGCC AGGGACTCCA TCGCTAAAGT GGCCTACGGT 
CGAGTGTTTG GGTGGATCGT CAGTAAAATC AACGAACAGC TCGCTCCAAA AGTAGACTTT 
GATGTGGAAC TCAACGAAAT AGGAATCCTC GACATCTTTG GCTTTGAAAA CTTTGCTGTA 
AACCGATTTG AGCAGCTCTG CATTAATCTG GCTAATGAAC AGCTCCAGTA TTTCTTCAAC 
CACCACATTT TTCTGATGGA GCAGAAGGAA TATAAAGAGG AAGGAATCAC GTGGGAGACC 
ATAACCTACA AGGACAACAA ACCCATACTG GACCTGTTTC TCATGAAGCC CATTGGGATT 
CTCTCACTGC TGGATGAACA AAGTGCCTTT CCTCAGGCCA CTGATCGAGA TTTTGTTGAC 
AAGTTAAACA GCAAGTTTAA ATCGGCTCCA AACTTCGAAG TGGTGCGAAA TCACACTCCT 
CTGTTCACTG TTGTGCACTA CGCTGGGAAG GTGCAATATA ATGCGAGTGG TTTTCTGGAG 
AAAAATAGAG ACACGATTCC AGCCAGCATT CGCGAGCTCT TCATTAACAG CGTCACTCCT 
CTCCTCAGCG TTTTATTCGC AGCCACAATC TCCAGAACGG GCACTCTAAT GCCAAGGAAA 
GCCAAGTTGC AAGTTTCAGA TGATAACTTT AACTCCACCA GGAAGCAGTC TGTTGGTGCA 
CAGTTCAAGC ATTCTCTGGC GGTTCTGATG GAGAAGATGT TTGCTGCCAG TCCTCACTTC 
GTCCGCTGCA TCAAACCCAA CCGCAGCAAG CAGCCCGATC AGCTGGACAG TAAACTGGTC 
CTGGATCAGC TGCGGTACAA TGGACTGATG GAAACGATCC GGATCCGCCG GGATGGTTTC 
TCGTGGCGTC CGACATTTAG GGAGTTTGCC GAGAGGTACA ACATTCTGCT GCTGAAACCG 
GATCTGCCCA CAGACAAGAG CAGTTGTATG TCAATCCTCA ACAGCACAGA ACTGTCAGGA 
TGGAAGTGCG GATCATCGCG GCTGTTTTTT AAGCTCTGCC ACCAGGTGGA GTTGGCCAAT 
TTGCTGGACA GACTGGGCAG AGCGGCGCTT GTCATTCAGA AAAATTTCCG AGCCCGGACC 
TGCCGCAGGA AGTATCTGTG TCTGCTGGCG GAGCTGCGGC GTCAGCGTCA GGCTCAGCGG 
GAGCGCGAGG AGCGTGAGGA GGAGGAGAGG AGACGCCAGG AGGAGGAGAA GAGACGGCAG 
CAGCAGCAGG AGGCGGAGAG GAAGAGGCGT CTGGAGGTGG AGCAGGAGAA CCTGAGACCA 
GCGTCTCCAC CGGTGCCTTT GCCCAGGAAG AAAAAGCCGG AGCCCAGGCC TCGCTCGGTC 
CTCAGCAGCA TCAGTAAAAC AGCAAGCGTG GCTCCAGTAC CACGACCCCG CAGCAAGCTT 
TTAGAGGCGA CTCCATTTGA CAACGATCTG AACGAGAACT TCCACCTGGA CGCGTCTCTG 
ATGCTGCAGC CAATCGATGA AGAGCGCATT AAAGAGAAGC ACCTGAAGAA GAGCAACACT 
ATTCGCTGGT TTAGAGAGAC GCAGGTGCGC AAGCTGACCC GGGATGGCTC TTTCCCACAC 
TGGTTCCACG GCATGATCAC ACGGAGGCAA GCGGAAGATC TTCTCATCGA CAAACCTCTA 
GGCTGCTTCT TGGTGCGAGT CGGTCAAAGT CGTGAGGGTT TCACTCTGAC ATTCAGAGGT 
GCCGATCGTT GTCGTCACTA CATGGTGGAG ATGCAGAGCA ATGGGAAGTA TGTGATCCTG 
GGTGAAGATC GAGCTCATTC TTCTCTCACA GACCTGGTCC AGTACCACAC TCAGGTGGGC 
ATCAAGCCCT TCATGGAGCT GCTGACGGTG CCATGTGGAC AGATGTGCGA TCAGGAGCCG 
GACTATGAGG AGCTCAAGGC TTTTTCCTCG GCGGTGGAGA GCGTTGACGG TCCCGCTGAA 
AACATGGACA CGGGGTCAAA TAAAGTGCAC TTGCAGATGC AGAGACTGTT CCTGCCACCA 
GGAGGAAACT CAAACCATGA GCACCACCGA GCCCCAGTGC TGAAGCGCAT CTCTGACCGC 
CGCAGACGAA TGGAGGCGGC ACAAACCGAA CGCAAGGAGG AGGATCTGAC CAATCAGAGC 
AGGCCTTTCC CTCGACTCTA CCCATCAATA CGGCTAGCCA TGAGGGAGAT CCAGCAAATC 
CAAGAGCAGA GCCAAGAACA GATCCAGATT CCCTCCAGCA AAGACCTGAG CTCCTGGAAG 
AGGACGGATT CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_685035.4
CDS	91..3465
Translation	MKVTERGYKHELLICNERQSDLKFVSSRRSLDREIMEENLPEDLANLSELDEKCLLEVLTGRFTQNLIYTYIGDILVAINPFKYLSIYEKEVSQRYKCHEKKSLPPHIFAVADRAYQSMLGRLAMGPKNQCIVISGESGAGKTESTKLLLRHIMELCKANSQLEQQILQVNPLLEAFGNAQTVMNDNSSRFGKYIQLRFQNSSVKGAKINEYLLEKSRVVHQDQGEQNFHIFYLMLAGISLEDKEIYGLLDPTLYRYLNGRFGQEDVVQTWRVKYAEVCNAMDMVGFEEQEKVDMMTILAGILSLGNITFETTDSDALKVSEASRGWLKATAGQFGVQEEDLLKSLTCTTSMTRGEAIQRLHNQQQAEDARDSIAKVAYGRVFGWIVSKINEQLAPKVDFDVELNEIGILDIFGFENFAVNRFEQLCINLANEQLQYFFNHHIFLMEQKEYKEEGITWETITYKDNKPILDLFLMKPIGILSLLDEQSAFPQATDRDFVDKLNSKFKSAPNFEVVRNHTPLFTVVHYAGKVQYNASGFLEKNRDTIPASIRELFINSVTPLLSVLFAATISRTGTLMPRKAKLQVSDDNFNSTRKQSVGAQFKHSLAVLMEKMFAASPHFVRCIKPNRSKQPDQLDSKLVLDQLRYNGLMETIRIRRDGFSWRPTFREFAERYNILLLKPDLPTDKSSCMSILNSTELSGWKCGSSRLFFKLCHQVELANLLDRLGRAALVIQKNFRARTCRRKYLCLLAELRRQRQAQREREEREEEERRRQEEEKRRQQQQEAERKRRLEVEQENLRPASPPVPLPRKKKPEPRPRSVLSSISKTASVAPVPRPRSKLLEATPFDNDLNENFHLDASLMLQPIDEERIKEKHLKKSNTIRWFRETQVRKLTRDGSFPHWFHGMITRRQAEDLLIDKPLGCFLVRVGQSREGFTLTFRGADRCRHYMVEMQSNGKYVILGEDRAHSSLTDLVQYHTQVGIKPFMELLTVPCGQMCDQEPDYEELKAFSSAVESVDGPAENMDTGSNKVHLQMQRLFLPPGGNSNHEHHRAPVLKRISDRRRRMEAAQTERKEEDLTNQSRPFPRLYPSIRLAMREIQQIQEQSQEQIQIPSSKDLSSWKRTDS

Target ORF information:

RefSeq Version	XM_679943.7
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin-IIIb (LOC556989), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_679943.7

ORF Insert Sequence:

ATGAAAGTGA CCGAGCGTGG ATATAAACAC GAGCTGCTGA TTTGTAATGA AAGACAGTCA 
GATTTGAAGT TTGTAAGTTC ACGTAGATCA TTAGACAGAG AGATAATGGA GGAAAACCTG 
CCTGAAGATC TGGCGAATCT GTCTGAGCTG GACGAGAAAT GTTTGCTGGA GGTTTTGACT 
GGACGTTTCA CACAGAACCT CATCTATACG TATATAGGGG ACATCTTGGT GGCTATCAAT 
CCATTTAAAT ATCTTTCCAT CTATGAAAAA GAGGTATCCC AGCGTTATAA GTGCCATGAG 
AAGAAGTCTT TACCCCCGCA TATATTCGCT GTGGCGGACC GAGCCTATCA GTCCATGCTG 
GGACGTCTAG CTATGGGACC TAAGAATCAA TGCATCGTCA TCAGTGGTGA GAGCGGCGCG 
GGTAAAACGG AGAGCACCAA ACTGCTGCTG AGACACATCA TGGAGCTCTG CAAGGCCAAC 
TCCCAGCTGG AGCAGCAGAT CCTGCAGGTG AATCCGCTTC TGGAGGCCTT TGGGAATGCT 
CAGACGGTCA TGAATGACAA CAGCAGTCGC TTCGGGAAGT ACATCCAGCT CCGTTTCCAG 
AACTCCTCAG TTAAAGGAGC CAAAATCAAT GAGTACCTGC TGGAGAAATC TCGCGTGGTC 
CACCAGGACC AGGGGGAGCA GAACTTCCAC ATCTTCTACC TCATGTTGGC TGGAATTTCA 
TTGGAGGATA AGGAAATTTA CGGCCTTCTG GACCCGACGC TGTACCGGTA TTTGAATGGC 
AGGTTTGGGC AAGAAGACGT AGTGCAGACG TGGCGCGTGA AATATGCAGA GGTGTGTAAC 
GCCATGGACA TGGTAGGATT CGAAGAGCAG GAGAAAGTGG ACATGATGAC CATTCTCGCT 
GGCATTCTGT CTCTGGGAAA CATCACGTTC GAGACGACCG ACAGTGACGC TCTGAAAGTG 
TCTGAAGCGT CACGTGGATG GCTGAAAGCT ACTGCTGGTC AGTTTGGCGT CCAGGAAGAA 
GACCTGCTGA AGAGTCTGAC CTGCACCACG TCAATGACTC GAGGAGAAGC CATTCAAAGA 
CTCCACAACC AGCAGCAGGC CGAGGATGCC AGGGACTCCA TCGCTAAAGT GGCCTACGGT 
CGAGTGTTTG GGTGGATCGT CAGTAAAATC AACGAACAGC TCGCTCCAAA AGTAGACTTT 
GATGTGGAAC TCAACGAAAT AGGAATCCTC GACATCTTTG GCTTTGAAAA CTTTGCTGTA 
AACCGATTTG AGCAGCTCTG CATTAATCTG GCTAATGAAC AGCTCCAGTA TTTCTTCAAC 
CACCACATTT TTCTGATGGA GCAGAAGGAA TATAAAGAGG AAGGAATCAC GTGGGAGACC 
ATAACCTACA AGGACAACAA ACCCATACTG GACCTGTTTC TCATGAAGCC CATTGGGATT 
CTCTCACTGC TGGATGAACA AAGTGCCTTT CCTCAGGCCA CTGATCGAGA TTTTGTTGAC 
AAGTTAAACA GCAAGTTTAA ATCGGCTCCA AACTTCGAAG TGGTGCGAAA TCACACTCCT 
CTGTTCACTG TTGTGCACTA CGCTGGGAAG GTGCAATATA ATGCGAGTGG TTTTCTGGAG 
AAAAATAGAG ACACGATTCC AGCCAGCATT CGCGAGCTCT TCATTAACAG CGTCACTCCT 
CTCCTCAGCG TTTTATTCGC AGCCACAATC TCCAGAACGG GCACTCTAAT GCCAAGGAAA 
GCCAAGTTGC AAGTTTCAGA TGATAACTTT AACTCCACCA GGAAGCAGTC TGTTGGTGCA 
CAGTTCAAGC ATTCTCTGGC GGTTCTGATG GAGAAGATGT TTGCTGCCAG TCCTCACTTC 
GTCCGCTGCA TCAAACCCAA CCGCAGCAAG CAGCCCGATC AGCTGGACAG TAAACTGGTC 
CTGGATCAGC TGCGGTACAA TGGACTGATG GAAACGATCC GGATCCGCCG GGATGGTTTC 
TCGTGGCGTC CGACATTTAG GGAGTTTGCC GAGAGGTACA ACATTCTGCT GCTGAAACCG 
GATCTGCCCA CAGACAAGAG CAGTTGTATG TCAATCCTCA ACAGCACAGA ACTGTCAGGA 
TGGAAGTGCG GATCATCGCG GCTGTTTTTT AAGCTCTGCC ACCAGGTGGA GTTGGCCAAT 
TTGCTGGACA GACTGGGCAG AGCGGCGCTT GTCATTCAGA AAAATTTCCG AGCCCGGACC 
TGCCGCAGGA AGTATCTGTG TCTGCTGGCG GAGCTGCGGC GTCAGCGTCA GGCTCAGCGG 
GAGCGCGAGG AGCGTGAGGA GGAGGAGAGG AGACGCCAGG AGGAGGAGAA GAGACGGCAG 
CAGCAGCAGG AGGCGGAGAG GAAGAGGCGT CTGGAGGTGG AGCAGGAGAA CCTGAGACCA 
GCGTCTCCAC CGGTGCCTTT GCCCAGGAAG AAAAAGCCGG AGCCCAGGCC TCGCTCGGTC 
CTCAGCAGCA TCAGTAAAAC AGCAAGCGTG GCTCCAGTAC CACGACCCCG CAGCAAGCTT 
TTAGAGGCGA CTCCATTTGA CAACGATCTG AACGAGAACT TCCACCTGGA CGCGTCTCTG 
ATGCTGCAGC CAATCGATGA AGAGCGCATT AAAGAGAAGC ACCTGAAGAA GAGCAACACT 
ATTCGCTGGT TTAGAGAGAC GCAGGTGCGC AAGCTGACCC GGGATGGCTC TTTCCCACAC 
TGGTTCCACG GCATGATCAC ACGGAGGCAA GCGGAAGATC TTCTCATCGA CAAACCTCTA 
GGCTGCTTCT TGGTGCGAGT CGGTCAAAGT CGTGAGGGTT TCACTCTGAC ATTCAGAGGT 
GCCGATCGTT GTCGTCACTA CATGGTGGAG ATGCAGAGCA ATGGGAAGTA TGTGATCCTG 
GGTGAAGATC GAGCTCATTC TTCTCTCACA GACCTGGTCC AGTACCACAC TCAGGTGGGC 
ATCAAGCCCT TCATGGAGCT GCTGACGGTG CCATGTGGAC AGATGTGCGA TCAGGAGCCG 
GACTATGAGG AGCTCAAGGC TTTTTCCTCG GCGGTGGAGA GCGTTGACGG TCCCGCTGAA 
AACATGGACA CGGGGTCAAA TAAAGTGCAC TTGCAGATGC AGAGACTGTT CCTGCCACCA 
GGAGGAAACT CAAACCATGA GCACCACCGA GCCCCAGTGC TGAAGCGCAT CTCTGACCGC 
CGCAGACGAA TGGAGGCGGC ACAAACCGAA CGCAAGGAGG AGGATCTGAC CAATCAGAGC 
AGGCCTTTCC CTCGACTCTA CCCATCAATA CGGCTAGCCA TGAGGGAGAT CCAGCAAATC 
CAAGAGCAGA GCCAAGAACA GATCCAGATT CCCTCCAGCA AAGACCTGAG CTCCTGGAAG 
AGGACGGATT CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC556989 ( XM_005172588.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172645.1 Danio rerio myosin-IIIb (LOC556989), transcript variant X2, mRNA.

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CloneID	ODa15680
Clone ID Related Accession (Same CDS sequence)	XM_005172588.3 , XM_005172588.4
Accession Version	XM_005172588.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	myosin-IIIb isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172588.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTGA CCGAGCGTGG ATATAAACAC GAGCTGCTGA TTTGTAATGA AAGACAGTCA 
GATTTGAAGT TTGTAAGTTC ACGTAGATCA TTAGACAGAG AGATAATGGA GGAAAACCTG 
CCTGAAGATC TGGCGAATCT GTCTGAGCTG GACGAGAAAT GTTTGCTGGA GGTTTTGACT 
GGACGTTTCA CACAGAACCT CATCTATACG TATATAGGGG ACATCTTGGT GGCTATCAAT 
CCATTTAAAT ATCTTTCCAT CTATGAAAAA GAGGTATCCC AGCGTTATAA GTGCCATGAG 
AAGAAGTCTT TACCCCCGCA TATATTCGCT GTGGCGGACC GAGCCTATCA GTCCATGCTG 
GGACGTCTAG CTATGGGACC TAAGAATCAA TGCATCGTCA TCAGTGGTGA GAGCGGCGCG 
GGTAAAACGG AGAGCACCAA ACTGCTGCTG AGACACATCA TGGAGCTCTG CAAGGCCAAC 
TCCCAGCTGG AGCAGCAGAT CCTGCAGGTG AATCCGCTTC TGGAGGCCTT TGGGAATGCT 
CAGACGGTCA TGAATGACAA CAGCAGTCGC TTCGGGAAGT ACATCCAGCT CCGTTTCCAG 
AACTCCTCAG TTAAAGGAGC CAAAATCAAT GAGTACCTGC TGGAGAAATC TCGCGTGGTC 
CACCAGGACC AGGGGGAGCA GAACTTCCAC ATCTTCTACC TCATGTTGGC TGGAATTTCA 
TTGGAGGATA AGGAAATTTA CGGCCTTCTG GACCCGACGC TGTACCGGTA TTTGAATGGC 
AGGTTTGGGC AAGAAGACGT AGTGCAGACG TGGCGCGTGA AATATGCAGA GGTGTGTAAC 
GCCATGGACA TGGTAGGATT CGAAGAGCAG GAGAAAGTGG ACATGATGAC CATTCTCGCT 
GGCATTCTGT CTCTGGGAAA CATCACGTTC GAGACGACCG ACAGTGACGC TCTGAAAGTG 
TCTGAAGCGT CACGTGGATG GCTGAAAGCT ACTGCTGGTC AGTTTGGCGT CCAGGAAGAA 
GACCTGCTGA AGAGTCTGAC CTGCACCACG TCAATGACTC GAGGAGAAGC CATTCAAAGA 
CTCCACAACC AGCAGCAGGC CGAGGATGCC AGGGACTCCA TCGCTAAAGT GGCCTACGGT 
CGAGTGTTTG GGTGGATCGT CAGTAAAATC AACGAACAGC TCGCTCCAAA AGTAGACTTT 
GATGTGGAAC TCAACGAAAT AGGAATCCTC GACATCTTTG GCTTTGAAAA CTTTGCTGTA 
AACCGATTTG AGCAGCTCTG CATTAATCTG GCTAATGAAC AGCTCCAGTA TTTCTTCAAC 
CACCACATTT TTCTGATGGA GCAGAAGGAA TATAAAGAGG AAGGAATCAC GTGGGAGACC 
ATAACCTACA AGGACAACAA ACCCATACTG GACCTGTTTC TCATGAAGCC CATTGGGATT 
CTCTCACTGC TGGATGAACA AAGTGCCTTT CCTCAGGCCA CTGATCGAGA TTTTGTTGAC 
AAGTTAAACA GCAAGTTTAA ATCGGCTCCA AACTTCGAAG TGGTGCGAAA TCACACTCCT 
CTGTTCACTG TTGTGCACTA CGCTGGGAAG GTGCAATATA ATGCGAGTGG TTTTCTGGAG 
AAAAATAGAG ACACGATTCC AGCCAGCATT CGCGAGCTCT TCATTAACAG CGTCACTCCT 
CTCCTCAGCG TTTTATTCGC AGCCACAATC TCCAGAACGG GCACTCTAAT GCCAAGGAAA 
GCCAAGTTGC AAGTTTCAGA TGATAACTTT AACTCCACCA GGAAGCAGTC TGTTGGTGCA 
CAGTTCAAGC ATTCTCTGGC GGTTCTGATG GAGAAGATGT TTGCTGCCAG TCCTCACTTC 
GTCCGCTGCA TCAAACCCAA CCGCAGCAAG CAGCCCGATC AGCTGGACAG TAAACTGGTC 
CTGGATCAGC TGCGGTACAA TGGACTGATG GAAACGATCC GGATCCGCCG GGATGGTTTC 
TCGTGGCGTC CGACATTTAG GGAGTTTGCC GAGAGGTACA ACATTCTGCT GCTGAAACCG 
GATCTGCCCA CAGACAAGAG CAGTTGTATG TCAATCCTCA ACAGCACAGA ACTGTCAGGA 
TGGAAGTGCG GATCATCGCG GCTGTTTTTT AAGCTCTGCC ACCAGGTGGA GTTGGCCAAT 
TTGCTGGACA GACTGGGCAG AGCGGCGCTT GTCATTCAGA AAAATTTCCG AGCCCGGACC 
TGCCGCAGGA AGTATCTGTG TCTGCTGGCG GAGCTGCGGC GTCAGCGTCA GGCTCAGCGG 
GAGCGCGAGG AGCGTGAGGA GGAGGAGAGG AGACGCCAGG AGGAGGAGAA GAGACGGCAG 
CAGCAGCAGG AGGCGGAGAG GAAGAGGCGT CTGGAGGTGG AGCAGGAGAA CCTGAGACCA 
GCGTCTCCAC CGGTGCCTTT GCCCAGGAAG AAAAAGCCGG AGCCCAGGCC TCGCTCGGTC 
CTCAGCAGCA TCAGTAAAAC AGCAAGCGTG GCTCCAGTAC CACGACCCCG CAGCAAGCTT 
TTAGAGGCGA CTCCATTTGA CAACGATCTG AACGAGAACT TCCACCTGGA CGCGTCTCTG 
ATGCTGCAGC CAATCGATGA AGAGCGCATT AAAGAGAAGC ACCTGAAGAA GAGCAACACT 
ATTCGCTGGT TTAGAGAGAC GCAGGTGCGC AAGCTGACCC GGGATGGCTC TTTCCCACAC 
TGGTTCCACG GCATGATCAC ACGGAGGCAA GCGGAAGATC TTCTCATCGA CAAACCTCTA 
GGCTGCTTCT TGGTGCGAGT CGGTCAAAGT CGTGAGGGTT TCACTCTGAC ATTCAGAGGT 
GCCGATCGTT GTCGTCACTA CATGGTGGAG ATGCAGAGCA ATGGGAAGTA TGTGATCCTG 
GGTGAAGATC GAGCTCATTC TTCTCTCACA GACCTGGTCC AGTACCACAC TCAGGTGGGC 
ATCAAGCCCT TCATGGAGCT GCTGACGGTG CCATGTGGAC AGATGTGCGA TCAGGAGCCG 
GACTATGAGG AGCTCAAGGC TTTTTCCTCG GCGGTGGAGA GCGTTGACGG TCCCGCTGAA 
AACATGGACA CGGGGTCAAA TAAAGTGCAC TTGCAGATGC AGAGACTGTT CCTGCCACCA 
GGAGGAAACT CAAACCATGA GCACCACCGA GCCCCAGTGC TGAAGCGCAT CTCTGACCGC 
CGCAGACGAA TGGAGGCGGC ACAAACCGAA CGCAAGGAGG AGGATCTGAC CAATCAGAGC 
AGGCCTTTCC CTCGACTCTA CCCATCAATA CGGCTAGCCA TGAGGGAGAT CCAGCAAATC 
CAAGAGAGCC AAGAACAGAT CCAGATTCCC TCCAGCAAAG ACCTGAGCTC CTGGAAGAGG 
ACGGATTCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172645.1
CDS	368..3739
Translation	MKVTERGYKHELLICNERQSDLKFVSSRRSLDREIMEENLPEDLANLSELDEKCLLEVLTGRFTQNLIYTYIGDILVAINPFKYLSIYEKEVSQRYKCHEKKSLPPHIFAVADRAYQSMLGRLAMGPKNQCIVISGESGAGKTESTKLLLRHIMELCKANSQLEQQILQVNPLLEAFGNAQTVMNDNSSRFGKYIQLRFQNSSVKGAKINEYLLEKSRVVHQDQGEQNFHIFYLMLAGISLEDKEIYGLLDPTLYRYLNGRFGQEDVVQTWRVKYAEVCNAMDMVGFEEQEKVDMMTILAGILSLGNITFETTDSDALKVSEASRGWLKATAGQFGVQEEDLLKSLTCTTSMTRGEAIQRLHNQQQAEDARDSIAKVAYGRVFGWIVSKINEQLAPKVDFDVELNEIGILDIFGFENFAVNRFEQLCINLANEQLQYFFNHHIFLMEQKEYKEEGITWETITYKDNKPILDLFLMKPIGILSLLDEQSAFPQATDRDFVDKLNSKFKSAPNFEVVRNHTPLFTVVHYAGKVQYNASGFLEKNRDTIPASIRELFINSVTPLLSVLFAATISRTGTLMPRKAKLQVSDDNFNSTRKQSVGAQFKHSLAVLMEKMFAASPHFVRCIKPNRSKQPDQLDSKLVLDQLRYNGLMETIRIRRDGFSWRPTFREFAERYNILLLKPDLPTDKSSCMSILNSTELSGWKCGSSRLFFKLCHQVELANLLDRLGRAALVIQKNFRARTCRRKYLCLLAELRRQRQAQREREEREEEERRRQEEEKRRQQQQEAERKRRLEVEQENLRPASPPVPLPRKKKPEPRPRSVLSSISKTASVAPVPRPRSKLLEATPFDNDLNENFHLDASLMLQPIDEERIKEKHLKKSNTIRWFRETQVRKLTRDGSFPHWFHGMITRRQAEDLLIDKPLGCFLVRVGQSREGFTLTFRGADRCRHYMVEMQSNGKYVILGEDRAHSSLTDLVQYHTQVGIKPFMELLTVPCGQMCDQEPDYEELKAFSSAVESVDGPAENMDTGSNKVHLQMQRLFLPPGGNSNHEHHRAPVLKRISDRRRRMEAAQTERKEEDLTNQSRPFPRLYPSIRLAMREIQQIQESQEQIQIPSSKDLSSWKRTDS

Target ORF information:

RefSeq Version	XM_005172588.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio myosin-IIIb (LOC556989), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172588.3

ORF Insert Sequence:

ATGAAAGTGA CCGAGCGTGG ATATAAACAC GAGCTGCTGA TTTGTAATGA AAGACAGTCA 
GATTTGAAGT TTGTAAGTTC ACGTAGATCA TTAGACAGAG AGATAATGGA GGAAAACCTG 
CCTGAAGATC TGGCGAATCT GTCTGAGCTG GACGAGAAAT GTTTGCTGGA GGTTTTGACT 
GGACGTTTCA CACAGAACCT CATCTATACG TATATAGGGG ACATCTTGGT GGCTATCAAT 
CCATTTAAAT ATCTTTCCAT CTATGAAAAA GAGGTATCCC AGCGTTATAA GTGCCATGAG 
AAGAAGTCTT TACCCCCGCA TATATTCGCT GTGGCGGACC GAGCCTATCA GTCCATGCTG 
GGACGTCTAG CTATGGGACC TAAGAATCAA TGCATCGTCA TCAGTGGTGA GAGCGGCGCG 
GGTAAAACGG AGAGCACCAA ACTGCTGCTG AGACACATCA TGGAGCTCTG CAAGGCCAAC 
TCCCAGCTGG AGCAGCAGAT CCTGCAGGTG AATCCGCTTC TGGAGGCCTT TGGGAATGCT 
CAGACGGTCA TGAATGACAA CAGCAGTCGC TTCGGGAAGT ACATCCAGCT CCGTTTCCAG 
AACTCCTCAG TTAAAGGAGC CAAAATCAAT GAGTACCTGC TGGAGAAATC TCGCGTGGTC 
CACCAGGACC AGGGGGAGCA GAACTTCCAC ATCTTCTACC TCATGTTGGC TGGAATTTCA 
TTGGAGGATA AGGAAATTTA CGGCCTTCTG GACCCGACGC TGTACCGGTA TTTGAATGGC 
AGGTTTGGGC AAGAAGACGT AGTGCAGACG TGGCGCGTGA AATATGCAGA GGTGTGTAAC 
GCCATGGACA TGGTAGGATT CGAAGAGCAG GAGAAAGTGG ACATGATGAC CATTCTCGCT 
GGCATTCTGT CTCTGGGAAA CATCACGTTC GAGACGACCG ACAGTGACGC TCTGAAAGTG 
TCTGAAGCGT CACGTGGATG GCTGAAAGCT ACTGCTGGTC AGTTTGGCGT CCAGGAAGAA 
GACCTGCTGA AGAGTCTGAC CTGCACCACG TCAATGACTC GAGGAGAAGC CATTCAAAGA 
CTCCACAACC AGCAGCAGGC CGAGGATGCC AGGGACTCCA TCGCTAAAGT GGCCTACGGT 
CGAGTGTTTG GGTGGATCGT CAGTAAAATC AACGAACAGC TCGCTCCAAA AGTAGACTTT 
GATGTGGAAC TCAACGAAAT AGGAATCCTC GACATCTTTG GCTTTGAAAA CTTTGCTGTA 
AACCGATTTG AGCAGCTCTG CATTAATCTG GCTAATGAAC AGCTCCAGTA TTTCTTCAAC 
CACCACATTT TTCTGATGGA GCAGAAGGAA TATAAAGAGG AAGGAATCAC GTGGGAGACC 
ATAACCTACA AGGACAACAA ACCCATACTG GACCTGTTTC TCATGAAGCC CATTGGGATT 
CTCTCACTGC TGGATGAACA AAGTGCCTTT CCTCAGGCCA CTGATCGAGA TTTTGTTGAC 
AAGTTAAACA GCAAGTTTAA ATCGGCTCCA AACTTCGAAG TGGTGCGAAA TCACACTCCT 
CTGTTCACTG TTGTGCACTA CGCTGGGAAG GTGCAATATA ATGCGAGTGG TTTTCTGGAG 
AAAAATAGAG ACACGATTCC AGCCAGCATT CGCGAGCTCT TCATTAACAG CGTCACTCCT 
CTCCTCAGCG TTTTATTCGC AGCCACAATC TCCAGAACGG GCACTCTAAT GCCAAGGAAA 
GCCAAGTTGC AAGTTTCAGA TGATAACTTT AACTCCACCA GGAAGCAGTC TGTTGGTGCA 
CAGTTCAAGC ATTCTCTGGC GGTTCTGATG GAGAAGATGT TTGCTGCCAG TCCTCACTTC 
GTCCGCTGCA TCAAACCCAA CCGCAGCAAG CAGCCCGATC AGCTGGACAG TAAACTGGTC 
CTGGATCAGC TGCGGTACAA TGGACTGATG GAAACGATCC GGATCCGCCG GGATGGTTTC 
TCGTGGCGTC CGACATTTAG GGAGTTTGCC GAGAGGTACA ACATTCTGCT GCTGAAACCG 
GATCTGCCCA CAGACAAGAG CAGTTGTATG TCAATCCTCA ACAGCACAGA ACTGTCAGGA 
TGGAAGTGCG GATCATCGCG GCTGTTTTTT AAGCTCTGCC ACCAGGTGGA GTTGGCCAAT 
TTGCTGGACA GACTGGGCAG AGCGGCGCTT GTCATTCAGA AAAATTTCCG AGCCCGGACC 
TGCCGCAGGA AGTATCTGTG TCTGCTGGCG GAGCTGCGGC GTCAGCGTCA GGCTCAGCGG 
GAGCGCGAGG AGCGTGAGGA GGAGGAGAGG AGACGCCAGG AGGAGGAGAA GAGACGGCAG 
CAGCAGCAGG AGGCGGAGAG GAAGAGGCGT CTGGAGGTGG AGCAGGAGAA CCTGAGACCA 
GCGTCTCCAC CGGTGCCTTT GCCCAGGAAG AAAAAGCCGG AGCCCAGGCC TCGCTCGGTC 
CTCAGCAGCA TCAGTAAAAC AGCAAGCGTG GCTCCAGTAC CACGACCCCG CAGCAAGCTT 
TTAGAGGCGA CTCCATTTGA CAACGATCTG AACGAGAACT TCCACCTGGA CGCGTCTCTG 
ATGCTGCAGC CAATCGATGA AGAGCGCATT AAAGAGAAGC ACCTGAAGAA GAGCAACACT 
ATTCGCTGGT TTAGAGAGAC GCAGGTGCGC AAGCTGACCC GGGATGGCTC TTTCCCACAC 
TGGTTCCACG GCATGATCAC ACGGAGGCAA GCGGAAGATC TTCTCATCGA CAAACCTCTA 
GGCTGCTTCT TGGTGCGAGT CGGTCAAAGT CGTGAGGGTT TCACTCTGAC ATTCAGAGGT 
GCCGATCGTT GTCGTCACTA CATGGTGGAG ATGCAGAGCA ATGGGAAGTA TGTGATCCTG 
GGTGAAGATC GAGCTCATTC TTCTCTCACA GACCTGGTCC AGTACCACAC TCAGGTGGGC 
ATCAAGCCCT TCATGGAGCT GCTGACGGTG CCATGTGGAC AGATGTGCGA TCAGGAGCCG 
GACTATGAGG AGCTCAAGGC TTTTTCCTCG GCGGTGGAGA GCGTTGACGG TCCCGCTGAA 
AACATGGACA CGGGGTCAAA TAAAGTGCAC TTGCAGATGC AGAGACTGTT CCTGCCACCA 
GGAGGAAACT CAAACCATGA GCACCACCGA GCCCCAGTGC TGAAGCGCAT CTCTGACCGC 
CGCAGACGAA TGGAGGCGGC ACAAACCGAA CGCAAGGAGG AGGATCTGAC CAATCAGAGC 
AGGCCTTTCC CTCGACTCTA CCCATCAATA CGGCTAGCCA TGAGGGAGAT CCAGCAAATC 
CAAGAGAGCC AAGAACAGAT CCAGATTCCC TCCAGCAAAG ACCTGAGCTC CTGGAAGAGG 
ACGGATTCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC556989 ( XM_005172588.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172645.1 Danio rerio myosin-IIIb (LOC556989), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa15680
Clone ID Related Accession (Same CDS sequence)	XM_005172588.3 , XM_005172588.4
Accession Version	XM_005172588.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3372bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	myosin-IIIb isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007121.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172588.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAAGTGA CCGAGCGTGG ATATAAACAC GAGCTGCTGA TTTGTAATGA AAGACAGTCA 
GATTTGAAGT TTGTAAGTTC ACGTAGATCA TTAGACAGAG AGATAATGGA GGAAAACCTG 
CCTGAAGATC TGGCGAATCT GTCTGAGCTG GACGAGAAAT GTTTGCTGGA GGTTTTGACT 
GGACGTTTCA CACAGAACCT CATCTATACG TATATAGGGG ACATCTTGGT GGCTATCAAT 
CCATTTAAAT ATCTTTCCAT CTATGAAAAA GAGGTATCCC AGCGTTATAA GTGCCATGAG 
AAGAAGTCTT TACCCCCGCA TATATTCGCT GTGGCGGACC GAGCCTATCA GTCCATGCTG 
GGACGTCTAG CTATGGGACC TAAGAATCAA TGCATCGTCA TCAGTGGTGA GAGCGGCGCG 
GGTAAAACGG AGAGCACCAA ACTGCTGCTG AGACACATCA TGGAGCTCTG CAAGGCCAAC 
TCCCAGCTGG AGCAGCAGAT CCTGCAGGTG AATCCGCTTC TGGAGGCCTT TGGGAATGCT 
CAGACGGTCA TGAATGACAA CAGCAGTCGC TTCGGGAAGT ACATCCAGCT CCGTTTCCAG 
AACTCCTCAG TTAAAGGAGC CAAAATCAAT GAGTACCTGC TGGAGAAATC TCGCGTGGTC 
CACCAGGACC AGGGGGAGCA GAACTTCCAC ATCTTCTACC TCATGTTGGC TGGAATTTCA 
TTGGAGGATA AGGAAATTTA CGGCCTTCTG GACCCGACGC TGTACCGGTA TTTGAATGGC 
AGGTTTGGGC AAGAAGACGT AGTGCAGACG TGGCGCGTGA AATATGCAGA GGTGTGTAAC 
GCCATGGACA TGGTAGGATT CGAAGAGCAG GAGAAAGTGG ACATGATGAC CATTCTCGCT 
GGCATTCTGT CTCTGGGAAA CATCACGTTC GAGACGACCG ACAGTGACGC TCTGAAAGTG 
TCTGAAGCGT CACGTGGATG GCTGAAAGCT ACTGCTGGTC AGTTTGGCGT CCAGGAAGAA 
GACCTGCTGA AGAGTCTGAC CTGCACCACG TCAATGACTC GAGGAGAAGC CATTCAAAGA 
CTCCACAACC AGCAGCAGGC CGAGGATGCC AGGGACTCCA TCGCTAAAGT GGCCTACGGT 
CGAGTGTTTG GGTGGATCGT CAGTAAAATC AACGAACAGC TCGCTCCAAA AGTAGACTTT 
GATGTGGAAC TCAACGAAAT AGGAATCCTC GACATCTTTG GCTTTGAAAA CTTTGCTGTA 
AACCGATTTG AGCAGCTCTG CATTAATCTG GCTAATGAAC AGCTCCAGTA TTTCTTCAAC 
CACCACATTT TTCTGATGGA GCAGAAGGAA TATAAAGAGG AAGGAATCAC GTGGGAGACC 
ATAACCTACA AGGACAACAA ACCCATACTG GACCTGTTTC TCATGAAGCC CATTGGGATT 
CTCTCACTGC TGGATGAACA AAGTGCCTTT CCTCAGGCCA CTGATCGAGA TTTTGTTGAC 
AAGTTAAACA GCAAGTTTAA ATCGGCTCCA AACTTCGAAG TGGTGCGAAA TCACACTCCT 
CTGTTCACTG TTGTGCACTA CGCTGGGAAG GTGCAATATA ATGCGAGTGG TTTTCTGGAG 
AAAAATAGAG ACACGATTCC AGCCAGCATT CGCGAGCTCT TCATTAACAG CGTCACTCCT 
CTCCTCAGCG TTTTATTCGC AGCCACAATC TCCAGAACGG GCACTCTAAT GCCAAGGAAA 
GCCAAGTTGC AAGTTTCAGA TGATAACTTT AACTCCACCA GGAAGCAGTC TGTTGGTGCA 
CAGTTCAAGC ATTCTCTGGC GGTTCTGATG GAGAAGATGT TTGCTGCCAG TCCTCACTTC 
GTCCGCTGCA TCAAACCCAA CCGCAGCAAG CAGCCCGATC AGCTGGACAG TAAACTGGTC 
CTGGATCAGC TGCGGTACAA TGGACTGATG GAAACGATCC GGATCCGCCG GGATGGTTTC 
TCGTGGCGTC CGACATTTAG GGAGTTTGCC GAGAGGTACA ACATTCTGCT GCTGAAACCG 
GATCTGCCCA CAGACAAGAG CAGTTGTATG TCAATCCTCA ACAGCACAGA ACTGTCAGGA 
TGGAAGTGCG GATCATCGCG GCTGTTTTTT AAGCTCTGCC ACCAGGTGGA GTTGGCCAAT 
TTGCTGGACA GACTGGGCAG AGCGGCGCTT GTCATTCAGA AAAATTTCCG AGCCCGGACC 
TGCCGCAGGA AGTATCTGTG TCTGCTGGCG GAGCTGCGGC GTCAGCGTCA GGCTCAGCGG 
GAGCGCGAGG AGCGTGAGGA GGAGGAGAGG AGACGCCAGG AGGAGGAGAA GAGACGGCAG 
CAGCAGCAGG AGGCGGAGAG GAAGAGGCGT CTGGAGGTGG AGCAGGAGAA CCTGAGACCA 
GCGTCTCCAC CGGTGCCTTT GCCCAGGAAG AAAAAGCCGG AGCCCAGGCC TCGCTCGGTC 
CTCAGCAGCA TCAGTAAAAC AGCAAGCGTG GCTCCAGTAC CACGACCCCG CAGCAAGCTT 
TTAGAGGCGA CTCCATTTGA CAACGATCTG AACGAGAACT TCCACCTGGA CGCGTCTCTG 
ATGCTGCAGC CAATCGATGA AGAGCGCATT AAAGAGAAGC ACCTGAAGAA GAGCAACACT 
ATTCGCTGGT TTAGAGAGAC GCAGGTGCGC AAGCTGACCC GGGATGGCTC TTTCCCACAC 
TGGTTCCACG GCATGATCAC ACGGAGGCAA GCGGAAGATC TTCTCATCGA CAAACCTCTA 
GGCTGCTTCT TGGTGCGAGT CGGTCAAAGT CGTGAGGGTT TCACTCTGAC ATTCAGAGGT 
GCCGATCGTT GTCGTCACTA CATGGTGGAG ATGCAGAGCA ATGGGAAGTA TGTGATCCTG 
GGTGAAGATC GAGCTCATTC TTCTCTCACA GACCTGGTCC AGTACCACAC TCAGGTGGGC 
ATCAAGCCCT TCATGGAGCT GCTGACGGTG CCATGTGGAC AGATGTGCGA TCAGGAGCCG 
GACTATGAGG AGCTCAAGGC TTTTTCCTCG GCGGTGGAGA GCGTTGACGG TCCCGCTGAA 
AACATGGACA CGGGGTCAAA TAAAGTGCAC TTGCAGATGC AGAGACTGTT CCTGCCACCA 
GGAGGAAACT CAAACCATGA GCACCACCGA GCCCCAGTGC TGAAGCGCAT CTCTGACCGC 
CGCAGACGAA TGGAGGCGGC ACAAACCGAA CGCAAGGAGG AGGATCTGAC CAATCAGAGC 
AGGCCTTTCC CTCGACTCTA CCCATCAATA CGGCTAGCCA TGAGGGAGAT CCAGCAAATC 
CAAGAGAGCC AAGAACAGAT CCAGATTCCC TCCAGCAAAG ACCTGAGCTC CTGGAAGAGG 
ACGGATTCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172645.1
CDS	92..3463
Translation	MKVTERGYKHELLICNERQSDLKFVSSRRSLDREIMEENLPEDLANLSELDEKCLLEVLTGRFTQNLIYTYIGDILVAINPFKYLSIYEKEVSQRYKCHEKKSLPPHIFAVADRAYQSMLGRLAMGPKNQCIVISGESGAGKTESTKLLLRHIMELCKANSQLEQQILQVNPLLEAFGNAQTVMNDNSSRFGKYIQLRFQNSSVKGAKINEYLLEKSRVVHQDQGEQNFHIFYLMLAGISLEDKEIYGLLDPTLYRYLNGRFGQEDVVQTWRVKYAEVCNAMDMVGFEEQEKVDMMTILAGILSLGNITFETTDSDALKVSEASRGWLKATAGQFGVQEEDLLKSLTCTTSMTRGEAIQRLHNQQQAEDARDSIAKVAYGRVFGWIVSKINEQLAPKVDFDVELNEIGILDIFGFENFAVNRFEQLCINLANEQLQYFFNHHIFLMEQKEYKEEGITWETITYKDNKPILDLFLMKPIGILSLLDEQSAFPQATDRDFVDKLNSKFKSAPNFEVVRNHTPLFTVVHYAGKVQYNASGFLEKNRDTIPASIRELFINSVTPLLSVLFAATISRTGTLMPRKAKLQVSDDNFNSTRKQSVGAQFKHSLAVLMEKMFAASPHFVRCIKPNRSKQPDQLDSKLVLDQLRYNGLMETIRIRRDGFSWRPTFREFAERYNILLLKPDLPTDKSSCMSILNSTELSGWKCGSSRLFFKLCHQVELANLLDRLGRAALVIQKNFRARTCRRKYLCLLAELRRQRQAQREREEREEEERRRQEEEKRRQQQQEAERKRRLEVEQENLRPASPPVPLPRKKKPEPRPRSVLSSISKTASVAPVPRPRSKLLEATPFDNDLNENFHLDASLMLQPIDEERIKEKHLKKSNTIRWFRETQVRKLTRDGSFPHWFHGMITRRQAEDLLIDKPLGCFLVRVGQSREGFTLTFRGADRCRHYMVEMQSNGKYVILGEDRAHSSLTDLVQYHTQVGIKPFMELLTVPCGQMCDQEPDYEELKAFSSAVESVDGPAENMDTGSNKVHLQMQRLFLPPGGNSNHEHHRAPVLKRISDRRRRMEAAQTERKEEDLTNQSRPFPRLYPSIRLAMREIQQIQESQEQIQIPSSKDLSSWKRTDS

Target ORF information:

RefSeq Version	XM_005172588.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio myosin-IIIb (LOC556989), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172588.4

ORF Insert Sequence:

ATGAAAGTGA CCGAGCGTGG ATATAAACAC GAGCTGCTGA TTTGTAATGA AAGACAGTCA 
GATTTGAAGT TTGTAAGTTC ACGTAGATCA TTAGACAGAG AGATAATGGA GGAAAACCTG 
CCTGAAGATC TGGCGAATCT GTCTGAGCTG GACGAGAAAT GTTTGCTGGA GGTTTTGACT 
GGACGTTTCA CACAGAACCT CATCTATACG TATATAGGGG ACATCTTGGT GGCTATCAAT 
CCATTTAAAT ATCTTTCCAT CTATGAAAAA GAGGTATCCC AGCGTTATAA GTGCCATGAG 
AAGAAGTCTT TACCCCCGCA TATATTCGCT GTGGCGGACC GAGCCTATCA GTCCATGCTG 
GGACGTCTAG CTATGGGACC TAAGAATCAA TGCATCGTCA TCAGTGGTGA GAGCGGCGCG 
GGTAAAACGG AGAGCACCAA ACTGCTGCTG AGACACATCA TGGAGCTCTG CAAGGCCAAC 
TCCCAGCTGG AGCAGCAGAT CCTGCAGGTG AATCCGCTTC TGGAGGCCTT TGGGAATGCT 
CAGACGGTCA TGAATGACAA CAGCAGTCGC TTCGGGAAGT ACATCCAGCT CCGTTTCCAG 
AACTCCTCAG TTAAAGGAGC CAAAATCAAT GAGTACCTGC TGGAGAAATC TCGCGTGGTC 
CACCAGGACC AGGGGGAGCA GAACTTCCAC ATCTTCTACC TCATGTTGGC TGGAATTTCA 
TTGGAGGATA AGGAAATTTA CGGCCTTCTG GACCCGACGC TGTACCGGTA TTTGAATGGC 
AGGTTTGGGC AAGAAGACGT AGTGCAGACG TGGCGCGTGA AATATGCAGA GGTGTGTAAC 
GCCATGGACA TGGTAGGATT CGAAGAGCAG GAGAAAGTGG ACATGATGAC CATTCTCGCT 
GGCATTCTGT CTCTGGGAAA CATCACGTTC GAGACGACCG ACAGTGACGC TCTGAAAGTG 
TCTGAAGCGT CACGTGGATG GCTGAAAGCT ACTGCTGGTC AGTTTGGCGT CCAGGAAGAA 
GACCTGCTGA AGAGTCTGAC CTGCACCACG TCAATGACTC GAGGAGAAGC CATTCAAAGA 
CTCCACAACC AGCAGCAGGC CGAGGATGCC AGGGACTCCA TCGCTAAAGT GGCCTACGGT 
CGAGTGTTTG GGTGGATCGT CAGTAAAATC AACGAACAGC TCGCTCCAAA AGTAGACTTT 
GATGTGGAAC TCAACGAAAT AGGAATCCTC GACATCTTTG GCTTTGAAAA CTTTGCTGTA 
AACCGATTTG AGCAGCTCTG CATTAATCTG GCTAATGAAC AGCTCCAGTA TTTCTTCAAC 
CACCACATTT TTCTGATGGA GCAGAAGGAA TATAAAGAGG AAGGAATCAC GTGGGAGACC 
ATAACCTACA AGGACAACAA ACCCATACTG GACCTGTTTC TCATGAAGCC CATTGGGATT 
CTCTCACTGC TGGATGAACA AAGTGCCTTT CCTCAGGCCA CTGATCGAGA TTTTGTTGAC 
AAGTTAAACA GCAAGTTTAA ATCGGCTCCA AACTTCGAAG TGGTGCGAAA TCACACTCCT 
CTGTTCACTG TTGTGCACTA CGCTGGGAAG GTGCAATATA ATGCGAGTGG TTTTCTGGAG 
AAAAATAGAG ACACGATTCC AGCCAGCATT CGCGAGCTCT TCATTAACAG CGTCACTCCT 
CTCCTCAGCG TTTTATTCGC AGCCACAATC TCCAGAACGG GCACTCTAAT GCCAAGGAAA 
GCCAAGTTGC AAGTTTCAGA TGATAACTTT AACTCCACCA GGAAGCAGTC TGTTGGTGCA 
CAGTTCAAGC ATTCTCTGGC GGTTCTGATG GAGAAGATGT TTGCTGCCAG TCCTCACTTC 
GTCCGCTGCA TCAAACCCAA CCGCAGCAAG CAGCCCGATC AGCTGGACAG TAAACTGGTC 
CTGGATCAGC TGCGGTACAA TGGACTGATG GAAACGATCC GGATCCGCCG GGATGGTTTC 
TCGTGGCGTC CGACATTTAG GGAGTTTGCC GAGAGGTACA ACATTCTGCT GCTGAAACCG 
GATCTGCCCA CAGACAAGAG CAGTTGTATG TCAATCCTCA ACAGCACAGA ACTGTCAGGA 
TGGAAGTGCG GATCATCGCG GCTGTTTTTT AAGCTCTGCC ACCAGGTGGA GTTGGCCAAT 
TTGCTGGACA GACTGGGCAG AGCGGCGCTT GTCATTCAGA AAAATTTCCG AGCCCGGACC 
TGCCGCAGGA AGTATCTGTG TCTGCTGGCG GAGCTGCGGC GTCAGCGTCA GGCTCAGCGG 
GAGCGCGAGG AGCGTGAGGA GGAGGAGAGG AGACGCCAGG AGGAGGAGAA GAGACGGCAG 
CAGCAGCAGG AGGCGGAGAG GAAGAGGCGT CTGGAGGTGG AGCAGGAGAA CCTGAGACCA 
GCGTCTCCAC CGGTGCCTTT GCCCAGGAAG AAAAAGCCGG AGCCCAGGCC TCGCTCGGTC 
CTCAGCAGCA TCAGTAAAAC AGCAAGCGTG GCTCCAGTAC CACGACCCCG CAGCAAGCTT 
TTAGAGGCGA CTCCATTTGA CAACGATCTG AACGAGAACT TCCACCTGGA CGCGTCTCTG 
ATGCTGCAGC CAATCGATGA AGAGCGCATT AAAGAGAAGC ACCTGAAGAA GAGCAACACT 
ATTCGCTGGT TTAGAGAGAC GCAGGTGCGC AAGCTGACCC GGGATGGCTC TTTCCCACAC 
TGGTTCCACG GCATGATCAC ACGGAGGCAA GCGGAAGATC TTCTCATCGA CAAACCTCTA 
GGCTGCTTCT TGGTGCGAGT CGGTCAAAGT CGTGAGGGTT TCACTCTGAC ATTCAGAGGT 
GCCGATCGTT GTCGTCACTA CATGGTGGAG ATGCAGAGCA ATGGGAAGTA TGTGATCCTG 
GGTGAAGATC GAGCTCATTC TTCTCTCACA GACCTGGTCC AGTACCACAC TCAGGTGGGC 
ATCAAGCCCT TCATGGAGCT GCTGACGGTG CCATGTGGAC AGATGTGCGA TCAGGAGCCG 
GACTATGAGG AGCTCAAGGC TTTTTCCTCG GCGGTGGAGA GCGTTGACGG TCCCGCTGAA 
AACATGGACA CGGGGTCAAA TAAAGTGCAC TTGCAGATGC AGAGACTGTT CCTGCCACCA 
GGAGGAAACT CAAACCATGA GCACCACCGA GCCCCAGTGC TGAAGCGCAT CTCTGACCGC 
CGCAGACGAA TGGAGGCGGC ACAAACCGAA CGCAAGGAGG AGGATCTGAC CAATCAGAGC 
AGGCCTTTCC CTCGACTCTA CCCATCAATA CGGCTAGCCA TGAGGGAGAT CCAGCAAATC 
CAAGAGAGCC AAGAACAGAT CCAGATTCCC TCCAGCAAAG ACCTGAGCTC CTGGAAGAGG 
ACGGATTCAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC556989 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

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