phka2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	phka2
Entrez Gene ID	556199
Full Name	phosphorylase kinase, alpha 2 (liver)
General protein information	Preferred Names phosphorylase kinase, alpha 2 (liver) Names phosphorylase b kinase regulatory subunit alpha, liver isoform
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021479871.1	XP_021335546.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X1
XM_009306167.2	XP_009304442.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X1
XM_678952.9	XP_684044.4	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X3
XM_678952.8	XP_684044.4	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X2
XM_009306167.3	XP_009304442.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X2

Pathways from BioSystems

Homologies

	PHKA2	XP_001084454.1
Rattus norvegicus (Norway rat)	Phka2	NP_001177923.1
Danio rerio (zebrafish)	LOC556199	XP_684044.4
Drosophila melanogaster (fruit fly)	CG7766	NP_001138180.1
Pan troglodytes (chimpanzee)	PHKA2	XP_003317431.1
Mus musculus (house mouse)	Phka2	NP_001171350.1
Homo sapiens (human)	PHKA2	NP_000283.1
Bos taurus (cattle)	PHKA2	NP_001178474.1
Canis lupus familiaris (dog)	PHKA2	XP_005641224.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000708	XP_559348.4
Caenorhabditis elegans (roundworm)	C14B9.8	NP_498777.2
Gallus gallus (chicken)	PHKA2	XP_004934744.1
Xenopus tropicalis (tropical clawed frog)	phka2	XP_004911720.1

Gene Ontology

The following phka2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the phka2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa148035	XM_021479871.1 Latest version!	Danio rerio phosphorylase kinase, alpha 2 (liver) (phka2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa17230	XM_678952.9 Latest version!	Danio rerio phosphorylase kinase, alpha 2 (liver) (phka2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa42321	XM_009306167.3 Latest version!	Danio rerio phosphorylase kinase, alpha 2 (liver) (phka2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
View Old Accession Versions >>
ODa17230	XM_678952.8	Danio rerio phosphorylase kinase, alpha 2 (liver) (phka2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
ODa42321	XM_009306167.2	Danio rerio phosphorylase kinase, alpha 2 (liver) (phka2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

phka2 ( XM_021479871.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021335546.1 Danio rerio phosphorylase kinase, alpha 2 (liver) (phka2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa148035
Clone ID Related Accession (Same CDS sequence)	XM_021479871.1
Accession Version	XM_021479871.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3675bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCC GCAGTAATTC AGGAGTGAGG CTGGACGGAT TTGCCAGACT TGTCCATGAA 
ACAATATTAT GCCACCAGAA TCCAGTGACG GGTCTACTGC CGGCCAGCGA GCAGCAGAAG 
GATGCCTGGG TCAGGGATAA CGTGTACAGC ATTTTGGCAG TGTGGGGACT TGGGATGGCT 
TACCGCAAGA ATGCAGACCG TGATGAGGAT AAAGCCAAAG CCTATGAGCT TGAACAGAGT 
GTGGTTAAGC TAATGCAAGG TCTGCTGCAG TGTATGATGA GACAGGTGGA GAAGGTAGAG 
AAGTTCAAAC ACACACAGAG CACAAAGGAC TGCCTGCATG CCAAATATCA CACACCTACC 
TGTGCCACAG TGGTCGGAGA TGACCAATGG GGGCACCTGC AGGTCGATGC CACCTCACTC 
TACCTGCTCA CACTGGCCCA GATGACTGCC TCAGGTCTGC ACATCATATC AAACTTAGAC 
GAGGTGGCTT TCATCCAGAA CCTGGTGTTC TACATCGAAG CAGCGTACAA AGTGGCTGAT 
TATGGGATGT GGGAGCGAGG AGACAAGACC AATCAAGGCA TTCCTGAACT GAATGGCAGC 
TCCGTCGGAA TGGCAAAGGC AGCTTTGGAG GCCATAGATG AGCTTGATTT GTTTGGAGCT 
CATGGAGGTC CAAAATCAGT GATTCACGTT CTCCCAGATG AAGTAGAACA TTGTCAGTCT 
ATCTTGTGCT CCATGCTTCC TCGAGCCTCT ACCTCTAAAG AGATTGATGC TGGTCTGCTC 
TCTGTCATCT CATTTCCTGC CTTTGCTGTG GAGGATGCGG ATCTGGTCAA CATCACCAAG 
GCCGAAATCA TCACCAAGCT GCAGGGACGT TATGGCTGTT GTCGATTTAT CCGAGATGGA 
TACAGGACTC CAAAAGAGGA CCCTACTCGA CTTCACTATG ACCCTGCCGA GCTGAAGCTC 
TTTGAGAACA TTGAATGTGA ATGGCCAGTG TTTTGGACTT ATCTAATACT CGATGGCATC 
TTCAATGCAG ATCATGAACA GGTGGAGGAA TACAGAGAGG CTCTGGATGG GGTTTTGATC 
AGGGGCAAAC ATGGTATCCG TCTGGTGCCT GAACTCTACG CTGTACCTGC TGACAAAGTT 
GAGGAGGAGT ACAGGAACCC TCATTCGGTG GAGCGTGTGG CTTTTGGGCA GCTTCCACAC 
ATGTGGGGTC AGTCTCTGTA TATACTGGGC AGTCTCCTGA CTGAGGGATT TCTGGCCCCT 
GGTGAAATTG ATCCACTTAA TAGGCGGTTT TCTAAAGAAT TCAAGCCTGA TGTTGTTGTG 
CAAGTGAGTG TAGTGGCTGA GTCAGTGCAG ATCCAGCAGC TGCTGAGGGA TCATGGGATT 
GAGGTTCAGA CCGTCTCTGA TGTATCGCCC ATCCGGGTTA TGCCAGCTCG CATCCTGAGC 
CACATTTATG TGAAACTGGG AAACTGTAAA AAACTCAACT TGAGTGGACG ACCGTACAGG 
CACATTGGCG TTCTGGGAAC TTCAAAGTTT TATGAGATCA GAAATGGCAC TTACACTTTT 
ACTCCACAGT TCATTGACCA GCATCACTTC TACTTGGCTC TGGATAACCA GATGATTGTT 
GAGATGTTGC GGACAGAACT TGCTTACCTC TCGTCCTGCT GGCGAATGAC AGGAAGACCA 
ACCCTCACAT TCCCTATTAC ACAGAGCATG CTGGTTGAAG ATGGTGACAG TATTGACCCC 
TGCATCCTCT CCACCTTGCG GAAGCTTCAG GATGGCTACT TTGGTGGTGC AAGGGTGCAG 
ATGGCAAACC TGTCCTCGTT CCTCACCACC TCCTTCCACA CGCAGCTCAG CTTTTTGGAC 
GGCGACTCTG AAGAGGATCT GCTGGAGGAG GAAGAGGAGG AAGATGAAGA GGCTTCCGCA 
GGAGGATCAG GAGACATGTT CGACCAGTAC CTCACGGATT TATTGCACAG CACTACCACA 
AAATGCCATC TACCTCCCAT CCAAAGAGGG CAGCACCACG TTTTCAGTGC TGAACACACC 
ACCAGAGACA TACTGTCCTT CATGGCCCAA GTTCAAGGCC ACAACATGCC AAAGCCGTCC 
ATGTATTTGC CTGTTGCCCC TATCATGAGC AAACACAGGA AGTCTCTGAA CCTGCTAGAT 
GTCCCTCAAC ATAAAGCTTC ACCTCAGACA AAGGCTGATA ACGTTGACCT GCATTTACCA 
CGCGATTCTC ATGGAAACAC AGACTTTGTT CATCTGGTGG ATCAGCTTAA GCACTGTCCA 
ACCCTGCAGG ACCAGGCCGA TATCCTCTAT GTCCTCTATG CTATGAAAGG CCCGAACTGG 
GTGGTGAGTT TGTCAGGACA GGGTGAGGCG ACAGTTCGCT CTCTGCTGGA GGAGCTGTAC 
GTTCGGGCAG GAGCGTGTAA GGAATGGGGC CTCATCCGCT ACATCTCCGG CATCCTGAAG 
AAGAGGGTGG AAGTCCTTGC TGAAGCCTGC ACAGATCTGA TCTCTCATCA TAAACAGCTG 
ACAGTGGGTC TTCCTCCTGA ACCCAGAGAG AAAGTCATCA CGGTTCCTCT TCCTCCTGAA 
GAATTGATCA GCTTGATCTA CGAGGCCAGC GGACAAGACA TCAGCATTGC AGTCCTCACA 
CAGGAGATTA TGGTGTATTT GGCCATGTAC GTGCGCTCTC AGCCATCTCT GTTTGGAGAT 
ATGCTGCGTC TGCGCATCGG TCTCATCATG CAGGTGATGG CAACTGAACT GGCCCGCAGT 
CTGCACTGCT CAGGAGAAGA GGCTTCAGAG AGCCTGATGA GCCTCAGTCC TTTTGACATG 
AAGAATCTCT TACATCACAT CCTGAGCGGG AAAGAGTTTG GCGTTGAGAG GAGCATGCGT 
CCAATTCAGT CGTCTGCCAC CAGTCCTGCC ATCTCCATCC ATGAGCTCGG ACACACAGGA 
GCCACCAAGA CAGAGCGGAC AGGCATCAGA AAACTCAAGA CTGAAATTAA ACAGCTCGAT 
GACTCCAGAC CCATAAGCAT CTATTCCAGC AGTCATTCCA TCAGCAGTAA TGTCACATCA 
CCTCGCTCCA CGCGATGCAG CAGCCCATCC ACTCCCAGCG GGATTTTGTC TCCGACCGGG 
ACAGGAGACT GTCACCTGCA GTGGGAGGAG CGGCAGGGCC AGTGGCTGAG GAGACGCAGG 
TTGGACGGGG CCATCAACCG GGTCCCCATG GGCTTCTATC AGAAAGTCTG GAAGATTCTC 
CAAAAGTGTC ATGGCCTCTC CATCGCTGGT TATGTCCTGC CTTCTTCTAC CACCCGTGAG 
ATGACGGAGG GAGAGATCAA GTTTGCGGTG CATGTGGAGT CTGTCCTGAA TCATGTTCCT 
CAGCCGGAGT ACAGGCAGCT GCTGGTGGAG GCTATTATGG TGCTCACTCT TGTGGCCGAC 
ATGGAGATTC CTAGTGTTGG TGGAATTATT CACATCGACC GCATCGTTCA CATGGCCAAC 
GATCTCTTTC AGCAGGATCA GAGATCCAGC GGGGCCAATG AGTATTTCTT GGAGCAAGAT 
CCTGCCACAG GAATCTGTAA CTTCTTCTAT GACAGCGCTC CCAGCGGCAG CTATGGCACC 
ATGACTTATC TTTCCAAGGC GGTGGTCACA TATGTTCAAG ACTTCCTGCC AAACAGCAGC 
TGTCTAATGC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021335546.1
CDS	580..4254
Translation	MRSRSNSGVRLDGFARLVHETILCHQNPVTGLLPASEQQKDAWVRDNVYSILAVWGLGMAYRKNADRDEDKAKAYELEQSVVKLMQGLLQCMMRQVEKVEKFKHTQSTKDCLHAKYHTPTCATVVGDDQWGHLQVDATSLYLLTLAQMTASGLHIISNLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNGSSVGMAKAALEAIDELDLFGAHGGPKSVIHVLPDEVEHCQSILCSMLPRASTSKEIDAGLLSVISFPAFAVEDADLVNITKAEIITKLQGRYGCCRFIRDGYRTPKEDPTRLHYDPAELKLFENIECEWPVFWTYLILDGIFNADHEQVEEYREALDGVLIRGKHGIRLVPELYAVPADKVEEEYRNPHSVERVAFGQLPHMWGQSLYILGSLLTEGFLAPGEIDPLNRRFSKEFKPDVVVQVSVVAESVQIQQLLRDHGIEVQTVSDVSPIRVMPARILSHIYVKLGNCKKLNLSGRPYRHIGVLGTSKFYEIRNGTYTFTPQFIDQHHFYLALDNQMIVEMLRTELAYLSSCWRMTGRPTLTFPITQSMLVEDGDSIDPCILSTLRKLQDGYFGGARVQMANLSSFLTTSFHTQLSFLDGDSEEDLLEEEEEEDEEASAGGSGDMFDQYLTDLLHSTTTKCHLPPIQRGQHHVFSAEHTTRDILSFMAQVQGHNMPKPSMYLPVAPIMSKHRKSLNLLDVPQHKASPQTKADNVDLHLPRDSHGNTDFVHLVDQLKHCPTLQDQADILYVLYAMKGPNWVVSLSGQGEATVRSLLEELYVRAGACKEWGLIRYISGILKKRVEVLAEACTDLISHHKQLTVGLPPEPREKVITVPLPPEELISLIYEASGQDISIAVLTQEIMVYLAMYVRSQPSLFGDMLRLRIGLIMQVMATELARSLHCSGEEASESLMSLSPFDMKNLLHHILSGKEFGVERSMRPIQSSATSPAISIHELGHTGATKTERTGIRKLKTEIKQLDDSRPISIYSSSHSISSNVTSPRSTRCSSPSTPSGILSPTGTGDCHLQWEERQGQWLRRRRLDGAINRVPMGFYQKVWKILQKCHGLSIAGYVLPSSTTREMTEGEIKFAVHVESVLNHVPQPEYRQLLVEAIMVLTLVADMEIPSVGGIIHIDRIVHMANDLFQQDQRSSGANEYFLEQDPATGICNFFYDSAPSGSYGTMTYLSKAVVTYVQDFLPNSSCLMQ

Target ORF information:

RefSeq Version	XM_021479871.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphorylase kinase, alpha 2 (liver) (phka2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021479871.1

ORF Insert Sequence:

ATGAGGAGCC GCAGTAATTC AGGAGTGAGG CTGGACGGAT TTGCCAGACT TGTCCATGAA 
ACAATATTAT GCCACCAGAA TCCAGTGACG GGTCTACTGC CGGCCAGCGA GCAGCAGAAG 
GATGCCTGGG TCAGGGATAA CGTGTACAGC ATTTTGGCAG TGTGGGGACT TGGGATGGCT 
TACCGCAAGA ATGCAGACCG TGATGAGGAT AAAGCCAAAG CCTATGAGCT TGAACAGAGT 
GTGGTTAAGC TAATGCAAGG TCTGCTGCAG TGTATGATGA GACAGGTGGA GAAGGTAGAG 
AAGTTCAAAC ACACACAGAG CACAAAGGAC TGCCTGCATG CCAAATATCA CACACCTACC 
TGTGCCACAG TGGTCGGAGA TGACCAATGG GGGCACCTGC AGGTCGATGC CACCTCACTC 
TACCTGCTCA CACTGGCCCA GATGACTGCC TCAGGTCTGC ACATCATATC AAACTTAGAC 
GAGGTGGCTT TCATCCAGAA CCTGGTGTTC TACATCGAAG CAGCGTACAA AGTGGCTGAT 
TATGGGATGT GGGAGCGAGG AGACAAGACC AATCAAGGCA TTCCTGAACT GAATGGCAGC 
TCCGTCGGAA TGGCAAAGGC AGCTTTGGAG GCCATAGATG AGCTTGATTT GTTTGGAGCT 
CATGGAGGTC CAAAATCAGT GATTCACGTT CTCCCAGATG AAGTAGAACA TTGTCAGTCT 
ATCTTGTGCT CCATGCTTCC TCGAGCCTCT ACCTCTAAAG AGATTGATGC TGGTCTGCTC 
TCTGTCATCT CATTTCCTGC CTTTGCTGTG GAGGATGCGG ATCTGGTCAA CATCACCAAG 
GCCGAAATCA TCACCAAGCT GCAGGGACGT TATGGCTGTT GTCGATTTAT CCGAGATGGA 
TACAGGACTC CAAAAGAGGA CCCTACTCGA CTTCACTATG ACCCTGCCGA GCTGAAGCTC 
TTTGAGAACA TTGAATGTGA ATGGCCAGTG TTTTGGACTT ATCTAATACT CGATGGCATC 
TTCAATGCAG ATCATGAACA GGTGGAGGAA TACAGAGAGG CTCTGGATGG GGTTTTGATC 
AGGGGCAAAC ATGGTATCCG TCTGGTGCCT GAACTCTACG CTGTACCTGC TGACAAAGTT 
GAGGAGGAGT ACAGGAACCC TCATTCGGTG GAGCGTGTGG CTTTTGGGCA GCTTCCACAC 
ATGTGGGGTC AGTCTCTGTA TATACTGGGC AGTCTCCTGA CTGAGGGATT TCTGGCCCCT 
GGTGAAATTG ATCCACTTAA TAGGCGGTTT TCTAAAGAAT TCAAGCCTGA TGTTGTTGTG 
CAAGTGAGTG TAGTGGCTGA GTCAGTGCAG ATCCAGCAGC TGCTGAGGGA TCATGGGATT 
GAGGTTCAGA CCGTCTCTGA TGTATCGCCC ATCCGGGTTA TGCCAGCTCG CATCCTGAGC 
CACATTTATG TGAAACTGGG AAACTGTAAA AAACTCAACT TGAGTGGACG ACCGTACAGG 
CACATTGGCG TTCTGGGAAC TTCAAAGTTT TATGAGATCA GAAATGGCAC TTACACTTTT 
ACTCCACAGT TCATTGACCA GCATCACTTC TACTTGGCTC TGGATAACCA GATGATTGTT 
GAGATGTTGC GGACAGAACT TGCTTACCTC TCGTCCTGCT GGCGAATGAC AGGAAGACCA 
ACCCTCACAT TCCCTATTAC ACAGAGCATG CTGGTTGAAG ATGGTGACAG TATTGACCCC 
TGCATCCTCT CCACCTTGCG GAAGCTTCAG GATGGCTACT TTGGTGGTGC AAGGGTGCAG 
ATGGCAAACC TGTCCTCGTT CCTCACCACC TCCTTCCACA CGCAGCTCAG CTTTTTGGAC 
GGCGACTCTG AAGAGGATCT GCTGGAGGAG GAAGAGGAGG AAGATGAAGA GGCTTCCGCA 
GGAGGATCAG GAGACATGTT CGACCAGTAC CTCACGGATT TATTGCACAG CACTACCACA 
AAATGCCATC TACCTCCCAT CCAAAGAGGG CAGCACCACG TTTTCAGTGC TGAACACACC 
ACCAGAGACA TACTGTCCTT CATGGCCCAA GTTCAAGGCC ACAACATGCC AAAGCCGTCC 
ATGTATTTGC CTGTTGCCCC TATCATGAGC AAACACAGGA AGTCTCTGAA CCTGCTAGAT 
GTCCCTCAAC ATAAAGCTTC ACCTCAGACA AAGGCTGATA ACGTTGACCT GCATTTACCA 
CGCGATTCTC ATGGAAACAC AGACTTTGTT CATCTGGTGG ATCAGCTTAA GCACTGTCCA 
ACCCTGCAGG ACCAGGCCGA TATCCTCTAT GTCCTCTATG CTATGAAAGG CCCGAACTGG 
GTGGTGAGTT TGTCAGGACA GGGTGAGGCG ACAGTTCGCT CTCTGCTGGA GGAGCTGTAC 
GTTCGGGCAG GAGCGTGTAA GGAATGGGGC CTCATCCGCT ACATCTCCGG CATCCTGAAG 
AAGAGGGTGG AAGTCCTTGC TGAAGCCTGC ACAGATCTGA TCTCTCATCA TAAACAGCTG 
ACAGTGGGTC TTCCTCCTGA ACCCAGAGAG AAAGTCATCA CGGTTCCTCT TCCTCCTGAA 
GAATTGATCA GCTTGATCTA CGAGGCCAGC GGACAAGACA TCAGCATTGC AGTCCTCACA 
CAGGAGATTA TGGTGTATTT GGCCATGTAC GTGCGCTCTC AGCCATCTCT GTTTGGAGAT 
ATGCTGCGTC TGCGCATCGG TCTCATCATG CAGGTGATGG CAACTGAACT GGCCCGCAGT 
CTGCACTGCT CAGGAGAAGA GGCTTCAGAG AGCCTGATGA GCCTCAGTCC TTTTGACATG 
AAGAATCTCT TACATCACAT CCTGAGCGGG AAAGAGTTTG GCGTTGAGAG GAGCATGCGT 
CCAATTCAGT CGTCTGCCAC CAGTCCTGCC ATCTCCATCC ATGAGCTCGG ACACACAGGA 
GCCACCAAGA CAGAGCGGAC AGGCATCAGA AAACTCAAGA CTGAAATTAA ACAGCTCGAT 
GACTCCAGAC CCATAAGCAT CTATTCCAGC AGTCATTCCA TCAGCAGTAA TGTCACATCA 
CCTCGCTCCA CGCGATGCAG CAGCCCATCC ACTCCCAGCG GGATTTTGTC TCCGACCGGG 
ACAGGAGACT GTCACCTGCA GTGGGAGGAG CGGCAGGGCC AGTGGCTGAG GAGACGCAGG 
TTGGACGGGG CCATCAACCG GGTCCCCATG GGCTTCTATC AGAAAGTCTG GAAGATTCTC 
CAAAAGTGTC ATGGCCTCTC CATCGCTGGT TATGTCCTGC CTTCTTCTAC CACCCGTGAG 
ATGACGGAGG GAGAGATCAA GTTTGCGGTG CATGTGGAGT CTGTCCTGAA TCATGTTCCT 
CAGCCGGAGT ACAGGCAGCT GCTGGTGGAG GCTATTATGG TGCTCACTCT TGTGGCCGAC 
ATGGAGATTC CTAGTGTTGG TGGAATTATT CACATCGACC GCATCGTTCA CATGGCCAAC 
GATCTCTTTC AGCAGGATCA GAGATCCAGC GGGGCCAATG AGTATTTCTT GGAGCAAGAT 
CCTGCCACAG GAATCTGTAA CTTCTTCTAT GACAGCGCTC CCAGCGGCAG CTATGGCACC 
ATGACTTATC TTTCCAAGGC GGTGGTCACA TATGTTCAAG ACTTCCTGCC AAACAGCAGC 
TGTCTAATGC AGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phka2 ( XM_678952.9 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_684044.4 Danio rerio phosphorylase kinase, alpha 2 (liver) (phka2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa17230
Clone ID Related Accession (Same CDS sequence)	XM_678952.9 , XM_678952.8
Accession Version	XM_678952.9 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_678952.8. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCC GCAGTAATTC AGGAGTGAGG CTGGACGGAT TTGCCAGACT TGTCCATGAA 
ACAATATTAT GCCACCAGAA TCCAGTGACG GGTCTACTGC CGGCCAGCGA GCAGCAGAAG 
GATGCCTGGG TCAGGGATAA CGTGTACAGC ATTTTGGCAG TGTGGGGACT TGGGATGGCT 
TACCGCAAGA ATGCAGACCG TGATGAGGAT AAAGCCAAAG CCTATGAGCT TGAACAGAGT 
GTGGTTAAGC TAATGCAAGG TCTGCTGCAG TGTATGATGA GACAGGTGGA GAAGGTAGAG 
AAGTTCAAAC ACACACAGAG CACAAAGGAC TGCCTGCATG CCAAATATCA CACACCTACC 
TGTGCCACAG TGGTCGGAGA TGACCAATGG GGGCACCTGC AGGTCGATGC CACCTCACTC 
TACCTGCTCA CACTGGCCCA GATGACTGCC TCAGGTCTGC ACATCATATC AAACTTAGAC 
GAGGTGGCTT TCATCCAGAA CCTGGTGTTC TACATCGAAG CAGCGTACAA AGTGGCTGAT 
TATGGGATGT GGGAGCGAGG AGACAAGACC AATCAAGGCA TTCCTGAACT GAATGGCAGC 
TCCGTCGGAA TGGCAAAGGC AGCTTTGGAG GCCATAGATG AGCTTGATTT GTTTGGAGCT 
CATGGAGGTC CAAAATCAGT GATTCACGTT CTCCCAGATG AAGTAGAACA TTGTCAGTCT 
ATCTTGTGCT CCATGCTTCC TCGAGCCTCT ACCTCTAAAG AGATTGATGC TGGTCTGCTC 
TCTGTCATCT CATTTCCTGC CTTTGCTGTG GAGGATGCGG ATCTGGTCAA CATCACCAAG 
GCCGAAATCA TCACCAAGCT GCAGGGACGT TATGGCTGTT GTCGATTTAT CCGAGATGGA 
TACAGGACTC CAAAAGAGGA CCCTACTCGA CTTCACTATG ACCCTGCCGA GCTGAAGCTC 
TTTGAGAACA TTGAATGTGA ATGGCCAGTG TTTTGGACTT ATCTAATACT CGATGGCATC 
TTCAATGCAG ATCATGAACA GGTGGAGGAA TACAGAGAGG CTCTGGATGG GGTTTTGATC 
AGGGGCAAAC ATGGTATCCG TCTGGTGCCT GAACTCTACG CTGTACCTGC TGACAAAGTT 
GAGGAGGAGT ACAGGAACCC TCATTCGGTG GAGCGTGTGG CTTTTGGGCA GCTTCCACAC 
ATGTGGGGTC AGTCTCTGTA TATACTGGGC AGTCTCCTGA CTGAGGGATT TCTGGCCCCT 
GGTGAAATTG ATCCACTTAA TAGGCGGTTT TCTAAAGAAT TCAAGCCTGA TGTTGTTGTG 
CAAGTGAGTG TAGTGGCTGA GTCAGTGCAG ATCCAGCAGC TGCTGAGGGA TCATGGGATT 
GAGGTTCAGA CCGTCTCTGA TGTATCGCCC ATCCGGGTTA TGCCAGCTCG CATCCTGAGC 
CACATTTATG TGAAACTGGG AAACTGTAAA AAACTCAACT TGAGTGGACG ACCGTACAGG 
CACATTGGCG TTCTGGGAAC TTCAAAGTTT TATGAGATCA GAAATGGCAC TTACACTTTT 
ACTCCACAGT TCATTGACCA GCATCACTTC TACTTGGCTC TGGATAACCA GATGATTGTT 
GAGATGTTGC GGACAGAACT TGCTTACCTC TCGTCCTGCT GGCGAATGAC AGGAAGACCA 
ACCCTCACAT TCCCTATTAC ACAGAGCATG CTGGTTGAAG ATGGTGACAG TATTGACCCC 
TGCATCCTCT CCACCTTGCG GAAGCTTCAG GATGGCTACT TTGGTGGTGC AAGGGTGCAG 
ATGGCAAACC TGTCCTCGTT CCTCACCACC TCCTTCCACA CGCAGCTCAG CTTTTTGGAC 
GGCGACTCTG AAGAGGATCT GCTGGAGGAG GAAGAGGAGG AAGATGAAGA GGCTTCCGCA 
GGAGGATCAG GAGACATGTT CGACCAGTAC CTCACGGATT TATTGCACAG CACTACCACA 
AAATGCCATC TACCTCCCAT CCAAAGAGGG CAGCACCACG TTTTCAGTGC TGAACACACC 
ACCAGAGACA TACTGTCCTT CATGGCCCAA GTTCAAGGCC ACAACATGCC AAAGCCGTCC 
ATGTATTTGC CTGTTGCCCC TATCATGAGC AAACACAGGA AGTCTCTGAA CCTGCTAGAT 
GTCCCTCAAC ATAAAGCTTC ACCTCAGACA AAGGCTGATA ACGTTGACCT GCATTTACCA 
CGCGATTCTC ATGGAAACAC AGACTTTGTT CATCTGGTGG ATCAGCTTAA GCACTGTCCA 
ACCCTGCAGG ACCAGGCCGA TATCCTCTAT GTCCTCTATG CTATGAAAGG CCCGAACTGG 
GTGGTGAGTT TGTCAGGACA GGGTGAGGCG ACAGTTCGCT CTCTGCTGGA GGAGCTGTAC 
GTTCGGGCAG GAGCGTGTAA GGAATGGGGC CTCATCCGCT ACATCTCCGG CATCCTGAAG 
AAGAGGGTGG AAGTCCTTGC TGAAGCCTGC ACAGATCTGA TCTCTCATCA TAAACAGCTG 
ACAGTGGGTC TTCCTCCTGA ACCCAGAGAG AAAGTCATCA CGGTTCCTCT TCCTCCTGAA 
GAATTGATCA GCTTGATCTA CGAGGCCAGC GGACAAGACA TCAGCATTGC AGTCCTCACA 
CAGGAGATTA TGGTGTATTT GGCCATGTAC GTGCGCTCTC AGCCATCTCT GTTTGGAGAT 
ATGCTGCGTC TGCGCATCGG TCTCATCATG CAGGTGATGG CAACTGAACT GGCCCGCAGT 
CTGCACTGCT CAGGAGAAGA GGCTTCAGAG AGCCTGATGA GCCTCAGTCC TTTTGACATG 
AAGAATCTCT TACATCACAT CCTGAGCGGG AAAGAGTTTG GCGTTGAGAG GAGCATGCGT 
CCAATTCAGT CGTCTGCCAC CAGTCCTGCC ATCTCCATCC ATGAGCTCGG ACACACAGGA 
GCCACCAAGA CAGAGCGGAC AGGCATCAGA AAACTCAAGA CTGAAATTAA ACAGCTCGAT 
GACTCCAGAC CCATAAGCCG ATGCAGCAGC CCATCCACTC CCAGCGGGAT TTTGTCTCCG 
ACCGGGACAG GAGACTGTCA CCTGCAGTGG GAGGAGCGGC AGGGCCAGTG GCTGAGGAGA 
CGCAGGTTGG ACGGGGCCAT CAACCGGGTC CCCATGGGCT TCTATCAGAA AGTCTGGAAG 
ATTCTCCAAA AGTGTCATGG CCTCTCCATC GCTGGTTATG TCCTGCCTTC TTCTACCACC 
CGTGAGATGA CGGAGGGAGA GATCAAGTTT GCGGTGCATG TGGAGTCTGT CCTGAATCAT 
GTTCCTCAGC CGGAGTACAG GCAGCTGCTG GTGGAGGCTA TTATGGTGCT CACTCTTGTG 
GCCGACATGG AGATTCCTAG TGTTGGTGGA ATTATTCACA TCGACCGCAT CGTTCACATG 
GCCAACGATC TCTTTCAGCA GGATCAGAGA TCCAGCGGGG CCAATGAGTA TTTCTTGGAG 
CAAGATCCTG CCACAGGAAT CTGTAACTTC TTCTATGACA GCGCTCCCAG CGGCAGCTAT 
GGCACCATGA CTTATCTTTC CAAGGCGGTG GTCACATATG TTCAAGACTT CCTGCCAAAC 
AGCAGCTGTC TAATGCAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_684044.4
CDS	580..4200
Translation	MRSRSNSGVRLDGFARLVHETILCHQNPVTGLLPASEQQKDAWVRDNVYSILAVWGLGMAYRKNADRDEDKAKAYELEQSVVKLMQGLLQCMMRQVEKVEKFKHTQSTKDCLHAKYHTPTCATVVGDDQWGHLQVDATSLYLLTLAQMTASGLHIISNLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNGSSVGMAKAALEAIDELDLFGAHGGPKSVIHVLPDEVEHCQSILCSMLPRASTSKEIDAGLLSVISFPAFAVEDADLVNITKAEIITKLQGRYGCCRFIRDGYRTPKEDPTRLHYDPAELKLFENIECEWPVFWTYLILDGIFNADHEQVEEYREALDGVLIRGKHGIRLVPELYAVPADKVEEEYRNPHSVERVAFGQLPHMWGQSLYILGSLLTEGFLAPGEIDPLNRRFSKEFKPDVVVQVSVVAESVQIQQLLRDHGIEVQTVSDVSPIRVMPARILSHIYVKLGNCKKLNLSGRPYRHIGVLGTSKFYEIRNGTYTFTPQFIDQHHFYLALDNQMIVEMLRTELAYLSSCWRMTGRPTLTFPITQSMLVEDGDSIDPCILSTLRKLQDGYFGGARVQMANLSSFLTTSFHTQLSFLDGDSEEDLLEEEEEEDEEASAGGSGDMFDQYLTDLLHSTTTKCHLPPIQRGQHHVFSAEHTTRDILSFMAQVQGHNMPKPSMYLPVAPIMSKHRKSLNLLDVPQHKASPQTKADNVDLHLPRDSHGNTDFVHLVDQLKHCPTLQDQADILYVLYAMKGPNWVVSLSGQGEATVRSLLEELYVRAGACKEWGLIRYISGILKKRVEVLAEACTDLISHHKQLTVGLPPEPREKVITVPLPPEELISLIYEASGQDISIAVLTQEIMVYLAMYVRSQPSLFGDMLRLRIGLIMQVMATELARSLHCSGEEASESLMSLSPFDMKNLLHHILSGKEFGVERSMRPIQSSATSPAISIHELGHTGATKTERTGIRKLKTEIKQLDDSRPISRCSSPSTPSGILSPTGTGDCHLQWEERQGQWLRRRRLDGAINRVPMGFYQKVWKILQKCHGLSIAGYVLPSSTTREMTEGEIKFAVHVESVLNHVPQPEYRQLLVEAIMVLTLVADMEIPSVGGIIHIDRIVHMANDLFQQDQRSSGANEYFLEQDPATGICNFFYDSAPSGSYGTMTYLSKAVVTYVQDFLPNSSCLMQ

Target ORF information:

RefSeq Version	XM_678952.9
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphorylase kinase, alpha 2 (liver) (phka2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_678952.9

ORF Insert Sequence:

ATGAGGAGCC GCAGTAATTC AGGAGTGAGG CTGGACGGAT TTGCCAGACT TGTCCATGAA 
ACAATATTAT GCCACCAGAA TCCAGTGACG GGTCTACTGC CGGCCAGCGA GCAGCAGAAG 
GATGCCTGGG TCAGGGATAA CGTGTACAGC ATTTTGGCAG TGTGGGGACT TGGGATGGCT 
TACCGCAAGA ATGCAGACCG TGATGAGGAT AAAGCCAAAG CCTATGAGCT TGAACAGAGT 
GTGGTTAAGC TAATGCAAGG TCTGCTGCAG TGTATGATGA GACAGGTGGA GAAGGTAGAG 
AAGTTCAAAC ACACACAGAG CACAAAGGAC TGCCTGCATG CCAAATATCA CACACCTACC 
TGTGCCACAG TGGTCGGAGA TGACCAATGG GGGCACCTGC AGGTCGATGC CACCTCACTC 
TACCTGCTCA CACTGGCCCA GATGACTGCC TCAGGTCTGC ACATCATATC AAACTTAGAC 
GAGGTGGCTT TCATCCAGAA CCTGGTGTTC TACATCGAAG CAGCGTACAA AGTGGCTGAT 
TATGGGATGT GGGAGCGAGG AGACAAGACC AATCAAGGCA TTCCTGAACT GAATGGCAGC 
TCCGTCGGAA TGGCAAAGGC AGCTTTGGAG GCCATAGATG AGCTTGATTT GTTTGGAGCT 
CATGGAGGTC CAAAATCAGT GATTCACGTT CTCCCAGATG AAGTAGAACA TTGTCAGTCT 
ATCTTGTGCT CCATGCTTCC TCGAGCCTCT ACCTCTAAAG AGATTGATGC TGGTCTGCTC 
TCTGTCATCT CATTTCCTGC CTTTGCTGTG GAGGATGCGG ATCTGGTCAA CATCACCAAG 
GCCGAAATCA TCACCAAGCT GCAGGGACGT TATGGCTGTT GTCGATTTAT CCGAGATGGA 
TACAGGACTC CAAAAGAGGA CCCTACTCGA CTTCACTATG ACCCTGCCGA GCTGAAGCTC 
TTTGAGAACA TTGAATGTGA ATGGCCAGTG TTTTGGACTT ATCTAATACT CGATGGCATC 
TTCAATGCAG ATCATGAACA GGTGGAGGAA TACAGAGAGG CTCTGGATGG GGTTTTGATC 
AGGGGCAAAC ATGGTATCCG TCTGGTGCCT GAACTCTACG CTGTACCTGC TGACAAAGTT 
GAGGAGGAGT ACAGGAACCC TCATTCGGTG GAGCGTGTGG CTTTTGGGCA GCTTCCACAC 
ATGTGGGGTC AGTCTCTGTA TATACTGGGC AGTCTCCTGA CTGAGGGATT TCTGGCCCCT 
GGTGAAATTG ATCCACTTAA TAGGCGGTTT TCTAAAGAAT TCAAGCCTGA TGTTGTTGTG 
CAAGTGAGTG TAGTGGCTGA GTCAGTGCAG ATCCAGCAGC TGCTGAGGGA TCATGGGATT 
GAGGTTCAGA CCGTCTCTGA TGTATCGCCC ATCCGGGTTA TGCCAGCTCG CATCCTGAGC 
CACATTTATG TGAAACTGGG AAACTGTAAA AAACTCAACT TGAGTGGACG ACCGTACAGG 
CACATTGGCG TTCTGGGAAC TTCAAAGTTT TATGAGATCA GAAATGGCAC TTACACTTTT 
ACTCCACAGT TCATTGACCA GCATCACTTC TACTTGGCTC TGGATAACCA GATGATTGTT 
GAGATGTTGC GGACAGAACT TGCTTACCTC TCGTCCTGCT GGCGAATGAC AGGAAGACCA 
ACCCTCACAT TCCCTATTAC ACAGAGCATG CTGGTTGAAG ATGGTGACAG TATTGACCCC 
TGCATCCTCT CCACCTTGCG GAAGCTTCAG GATGGCTACT TTGGTGGTGC AAGGGTGCAG 
ATGGCAAACC TGTCCTCGTT CCTCACCACC TCCTTCCACA CGCAGCTCAG CTTTTTGGAC 
GGCGACTCTG AAGAGGATCT GCTGGAGGAG GAAGAGGAGG AAGATGAAGA GGCTTCCGCA 
GGAGGATCAG GAGACATGTT CGACCAGTAC CTCACGGATT TATTGCACAG CACTACCACA 
AAATGCCATC TACCTCCCAT CCAAAGAGGG CAGCACCACG TTTTCAGTGC TGAACACACC 
ACCAGAGACA TACTGTCCTT CATGGCCCAA GTTCAAGGCC ACAACATGCC AAAGCCGTCC 
ATGTATTTGC CTGTTGCCCC TATCATGAGC AAACACAGGA AGTCTCTGAA CCTGCTAGAT 
GTCCCTCAAC ATAAAGCTTC ACCTCAGACA AAGGCTGATA ACGTTGACCT GCATTTACCA 
CGCGATTCTC ATGGAAACAC AGACTTTGTT CATCTGGTGG ATCAGCTTAA GCACTGTCCA 
ACCCTGCAGG ACCAGGCCGA TATCCTCTAT GTCCTCTATG CTATGAAAGG CCCGAACTGG 
GTGGTGAGTT TGTCAGGACA GGGTGAGGCG ACAGTTCGCT CTCTGCTGGA GGAGCTGTAC 
GTTCGGGCAG GAGCGTGTAA GGAATGGGGC CTCATCCGCT ACATCTCCGG CATCCTGAAG 
AAGAGGGTGG AAGTCCTTGC TGAAGCCTGC ACAGATCTGA TCTCTCATCA TAAACAGCTG 
ACAGTGGGTC TTCCTCCTGA ACCCAGAGAG AAAGTCATCA CGGTTCCTCT TCCTCCTGAA 
GAATTGATCA GCTTGATCTA CGAGGCCAGC GGACAAGACA TCAGCATTGC AGTCCTCACA 
CAGGAGATTA TGGTGTATTT GGCCATGTAC GTGCGCTCTC AGCCATCTCT GTTTGGAGAT 
ATGCTGCGTC TGCGCATCGG TCTCATCATG CAGGTGATGG CAACTGAACT GGCCCGCAGT 
CTGCACTGCT CAGGAGAAGA GGCTTCAGAG AGCCTGATGA GCCTCAGTCC TTTTGACATG 
AAGAATCTCT TACATCACAT CCTGAGCGGG AAAGAGTTTG GCGTTGAGAG GAGCATGCGT 
CCAATTCAGT CGTCTGCCAC CAGTCCTGCC ATCTCCATCC ATGAGCTCGG ACACACAGGA 
GCCACCAAGA CAGAGCGGAC AGGCATCAGA AAACTCAAGA CTGAAATTAA ACAGCTCGAT 
GACTCCAGAC CCATAAGCCG ATGCAGCAGC CCATCCACTC CCAGCGGGAT TTTGTCTCCG 
ACCGGGACAG GAGACTGTCA CCTGCAGTGG GAGGAGCGGC AGGGCCAGTG GCTGAGGAGA 
CGCAGGTTGG ACGGGGCCAT CAACCGGGTC CCCATGGGCT TCTATCAGAA AGTCTGGAAG 
ATTCTCCAAA AGTGTCATGG CCTCTCCATC GCTGGTTATG TCCTGCCTTC TTCTACCACC 
CGTGAGATGA CGGAGGGAGA GATCAAGTTT GCGGTGCATG TGGAGTCTGT CCTGAATCAT 
GTTCCTCAGC CGGAGTACAG GCAGCTGCTG GTGGAGGCTA TTATGGTGCT CACTCTTGTG 
GCCGACATGG AGATTCCTAG TGTTGGTGGA ATTATTCACA TCGACCGCAT CGTTCACATG 
GCCAACGATC TCTTTCAGCA GGATCAGAGA TCCAGCGGGG CCAATGAGTA TTTCTTGGAG 
CAAGATCCTG CCACAGGAAT CTGTAACTTC TTCTATGACA GCGCTCCCAG CGGCAGCTAT 
GGCACCATGA CTTATCTTTC CAAGGCGGTG GTCACATATG TTCAAGACTT CCTGCCAAAC 
AGCAGCTGTC TAATGCAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phka2 ( XM_678952.8 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_684044.4 Danio rerio phosphorylase kinase, alpha 2 (liver) (phka2), transcript variant X2, mRNA.

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CloneID	ODa17230
Clone ID Related Accession (Same CDS sequence)	XM_678952.9 , XM_678952.8
Accession Version	XM_678952.8	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3621bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_678952.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCC GCAGTAATTC AGGAGTGAGG CTGGACGGAT TTGCCAGACT TGTCCATGAA 
ACAATATTAT GCCACCAGAA TCCAGTGACG GGTCTACTGC CGGCCAGCGA GCAGCAGAAG 
GATGCCTGGG TCAGGGATAA CGTGTACAGC ATTTTGGCAG TGTGGGGACT TGGGATGGCT 
TACCGCAAGA ATGCAGACCG TGATGAGGAT AAAGCCAAAG CCTATGAGCT TGAACAGAGT 
GTGGTTAAGC TAATGCAAGG TCTGCTGCAG TGTATGATGA GACAGGTGGA GAAGGTAGAG 
AAGTTCAAAC ACACACAGAG CACAAAGGAC TGCCTGCATG CCAAATATCA CACACCTACC 
TGTGCCACAG TGGTCGGAGA TGACCAATGG GGGCACCTGC AGGTCGATGC CACCTCACTC 
TACCTGCTCA CACTGGCCCA GATGACTGCC TCAGGTCTGC ACATCATATC AAACTTAGAC 
GAGGTGGCTT TCATCCAGAA CCTGGTGTTC TACATCGAAG CAGCGTACAA AGTGGCTGAT 
TATGGGATGT GGGAGCGAGG AGACAAGACC AATCAAGGCA TTCCTGAACT GAATGGCAGC 
TCCGTCGGAA TGGCAAAGGC AGCTTTGGAG GCCATAGATG AGCTTGATTT GTTTGGAGCT 
CATGGAGGTC CAAAATCAGT GATTCACGTT CTCCCAGATG AAGTAGAACA TTGTCAGTCT 
ATCTTGTGCT CCATGCTTCC TCGAGCCTCT ACCTCTAAAG AGATTGATGC TGGTCTGCTC 
TCTGTCATCT CATTTCCTGC CTTTGCTGTG GAGGATGCGG ATCTGGTCAA CATCACCAAG 
GCCGAAATCA TCACCAAGCT GCAGGGACGT TATGGCTGTT GTCGATTTAT CCGAGATGGA 
TACAGGACTC CAAAAGAGGA CCCTACTCGA CTTCACTATG ACCCTGCCGA GCTGAAGCTC 
TTTGAGAACA TTGAATGTGA ATGGCCAGTG TTTTGGACTT ATCTAATACT CGATGGCATC 
TTCAATGCAG ATCATGAACA GGTGGAGGAA TACAGAGAGG CTCTGGATGG GGTTTTGATC 
AGGGGCAAAC ATGGTATCCG TCTGGTGCCT GAACTCTACG CTGTACCTGC TGACAAAGTT 
GAGGAGGAGT ACAGGAACCC TCATTCGGTG GAGCGTGTGG CTTTTGGGCA GCTTCCACAC 
ATGTGGGGTC AGTCTCTGTA TATACTGGGC AGTCTCCTGA CTGAGGGATT TCTGGCCCCT 
GGTGAAATTG ATCCACTTAA TAGGCGGTTT TCTAAAGAAT TCAAGCCTGA TGTTGTTGTG 
CAAGTGAGTG TAGTGGCTGA GTCAGTGCAG ATCCAGCAGC TGCTGAGGGA TCATGGGATT 
GAGGTTCAGA CCGTCTCTGA TGTATCGCCC ATCCGGGTTA TGCCAGCTCG CATCCTGAGC 
CACATTTATG TGAAACTGGG AAACTGTAAA AAACTCAACT TGAGTGGACG ACCGTACAGG 
CACATTGGCG TTCTGGGAAC TTCAAAGTTT TATGAGATCA GAAATGGCAC TTACACTTTT 
ACTCCACAGT TCATTGACCA GCATCACTTC TACTTGGCTC TGGATAACCA GATGATTGTT 
GAGATGTTGC GGACAGAACT TGCTTACCTC TCGTCCTGCT GGCGAATGAC AGGAAGACCA 
ACCCTCACAT TCCCTATTAC ACAGAGCATG CTGGTTGAAG ATGGTGACAG TATTGACCCC 
TGCATCCTCT CCACCTTGCG GAAGCTTCAG GATGGCTACT TTGGTGGTGC AAGGGTGCAG 
ATGGCAAACC TGTCCTCGTT CCTCACCACC TCCTTCCACA CGCAGCTCAG CTTTTTGGAC 
GGCGACTCTG AAGAGGATCT GCTGGAGGAG GAAGAGGAGG AAGATGAAGA GGCTTCCGCA 
GGAGGATCAG GAGACATGTT CGACCAGTAC CTCACGGATT TATTGCACAG CACTACCACA 
AAATGCCATC TACCTCCCAT CCAAAGAGGG CAGCACCACG TTTTCAGTGC TGAACACACC 
ACCAGAGACA TACTGTCCTT CATGGCCCAA GTTCAAGGCC ACAACATGCC AAAGCCGTCC 
ATGTATTTGC CTGTTGCCCC TATCATGAGC AAACACAGGA AGTCTCTGAA CCTGCTAGAT 
GTCCCTCAAC ATAAAGCTTC ACCTCAGACA AAGGCTGATA ACGTTGACCT GCATTTACCA 
CGCGATTCTC ATGGAAACAC AGACTTTGTT CATCTGGTGG ATCAGCTTAA GCACTGTCCA 
ACCCTGCAGG ACCAGGCCGA TATCCTCTAT GTCCTCTATG CTATGAAAGG CCCGAACTGG 
GTGGTGAGTT TGTCAGGACA GGGTGAGGCG ACAGTTCGCT CTCTGCTGGA GGAGCTGTAC 
GTTCGGGCAG GAGCGTGTAA GGAATGGGGC CTCATCCGCT ACATCTCCGG CATCCTGAAG 
AAGAGGGTGG AAGTCCTTGC TGAAGCCTGC ACAGATCTGA TCTCTCATCA TAAACAGCTG 
ACAGTGGGTC TTCCTCCTGA ACCCAGAGAG AAAGTCATCA CGGTTCCTCT TCCTCCTGAA 
GAATTGATCA GCTTGATCTA CGAGGCCAGC GGACAAGACA TCAGCATTGC AGTCCTCACA 
CAGGAGATTA TGGTGTATTT GGCCATGTAC GTGCGCTCTC AGCCATCTCT GTTTGGAGAT 
ATGCTGCGTC TGCGCATCGG TCTCATCATG CAGGTGATGG CAACTGAACT GGCCCGCAGT 
CTGCACTGCT CAGGAGAAGA GGCTTCAGAG AGCCTGATGA GCCTCAGTCC TTTTGACATG 
AAGAATCTCT TACATCACAT CCTGAGCGGG AAAGAGTTTG GCGTTGAGAG GAGCATGCGT 
CCAATTCAGT CGTCTGCCAC CAGTCCTGCC ATCTCCATCC ATGAGCTCGG ACACACAGGA 
GCCACCAAGA CAGAGCGGAC AGGCATCAGA AAACTCAAGA CTGAAATTAA ACAGCTCGAT 
GACTCCAGAC CCATAAGCCG ATGCAGCAGC CCATCCACTC CCAGCGGGAT TTTGTCTCCG 
ACCGGGACAG GAGACTGTCA CCTGCAGTGG GAGGAGCGGC AGGGCCAGTG GCTGAGGAGA 
CGCAGGTTGG ACGGGGCCAT CAACCGGGTC CCCATGGGCT TCTATCAGAA AGTCTGGAAG 
ATTCTCCAAA AGTGTCATGG CCTCTCCATC GCTGGTTATG TCCTGCCTTC TTCTACCACC 
CGTGAGATGA CGGAGGGAGA GATCAAGTTT GCGGTGCATG TGGAGTCTGT CCTGAATCAT 
GTTCCTCAGC CGGAGTACAG GCAGCTGCTG GTGGAGGCTA TTATGGTGCT CACTCTTGTG 
GCCGACATGG AGATTCCTAG TGTTGGTGGA ATTATTCACA TCGACCGCAT CGTTCACATG 
GCCAACGATC TCTTTCAGCA GGATCAGAGA TCCAGCGGGG CCAATGAGTA TTTCTTGGAG 
CAAGATCCTG CCACAGGAAT CTGTAACTTC TTCTATGACA GCGCTCCCAG CGGCAGCTAT 
GGCACCATGA CTTATCTTTC CAAGGCGGTG GTCACATATG TTCAAGACTT CCTGCCAAAC 
AGCAGCTGTC TAATGCAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_684044.4
CDS	568..4188
Translation	MRSRSNSGVRLDGFARLVHETILCHQNPVTGLLPASEQQKDAWVRDNVYSILAVWGLGMAYRKNADRDEDKAKAYELEQSVVKLMQGLLQCMMRQVEKVEKFKHTQSTKDCLHAKYHTPTCATVVGDDQWGHLQVDATSLYLLTLAQMTASGLHIISNLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNGSSVGMAKAALEAIDELDLFGAHGGPKSVIHVLPDEVEHCQSILCSMLPRASTSKEIDAGLLSVISFPAFAVEDADLVNITKAEIITKLQGRYGCCRFIRDGYRTPKEDPTRLHYDPAELKLFENIECEWPVFWTYLILDGIFNADHEQVEEYREALDGVLIRGKHGIRLVPELYAVPADKVEEEYRNPHSVERVAFGQLPHMWGQSLYILGSLLTEGFLAPGEIDPLNRRFSKEFKPDVVVQVSVVAESVQIQQLLRDHGIEVQTVSDVSPIRVMPARILSHIYVKLGNCKKLNLSGRPYRHIGVLGTSKFYEIRNGTYTFTPQFIDQHHFYLALDNQMIVEMLRTELAYLSSCWRMTGRPTLTFPITQSMLVEDGDSIDPCILSTLRKLQDGYFGGARVQMANLSSFLTTSFHTQLSFLDGDSEEDLLEEEEEEDEEASAGGSGDMFDQYLTDLLHSTTTKCHLPPIQRGQHHVFSAEHTTRDILSFMAQVQGHNMPKPSMYLPVAPIMSKHRKSLNLLDVPQHKASPQTKADNVDLHLPRDSHGNTDFVHLVDQLKHCPTLQDQADILYVLYAMKGPNWVVSLSGQGEATVRSLLEELYVRAGACKEWGLIRYISGILKKRVEVLAEACTDLISHHKQLTVGLPPEPREKVITVPLPPEELISLIYEASGQDISIAVLTQEIMVYLAMYVRSQPSLFGDMLRLRIGLIMQVMATELARSLHCSGEEASESLMSLSPFDMKNLLHHILSGKEFGVERSMRPIQSSATSPAISIHELGHTGATKTERTGIRKLKTEIKQLDDSRPISRCSSPSTPSGILSPTGTGDCHLQWEERQGQWLRRRRLDGAINRVPMGFYQKVWKILQKCHGLSIAGYVLPSSTTREMTEGEIKFAVHVESVLNHVPQPEYRQLLVEAIMVLTLVADMEIPSVGGIIHIDRIVHMANDLFQQDQRSSGANEYFLEQDPATGICNFFYDSAPSGSYGTMTYLSKAVVTYVQDFLPNSSCLMQ

Target ORF information:

RefSeq Version	XM_678952.8
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phosphorylase kinase, alpha 2 (liver) (phka2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_678952.8

ORF Insert Sequence:

ATGAGGAGCC GCAGTAATTC AGGAGTGAGG CTGGACGGAT TTGCCAGACT TGTCCATGAA 
ACAATATTAT GCCACCAGAA TCCAGTGACG GGTCTACTGC CGGCCAGCGA GCAGCAGAAG 
GATGCCTGGG TCAGGGATAA CGTGTACAGC ATTTTGGCAG TGTGGGGACT TGGGATGGCT 
TACCGCAAGA ATGCAGACCG TGATGAGGAT AAAGCCAAAG CCTATGAGCT TGAACAGAGT 
GTGGTTAAGC TAATGCAAGG TCTGCTGCAG TGTATGATGA GACAGGTGGA GAAGGTAGAG 
AAGTTCAAAC ACACACAGAG CACAAAGGAC TGCCTGCATG CCAAATATCA CACACCTACC 
TGTGCCACAG TGGTCGGAGA TGACCAATGG GGGCACCTGC AGGTCGATGC CACCTCACTC 
TACCTGCTCA CACTGGCCCA GATGACTGCC TCAGGTCTGC ACATCATATC AAACTTAGAC 
GAGGTGGCTT TCATCCAGAA CCTGGTGTTC TACATCGAAG CAGCGTACAA AGTGGCTGAT 
TATGGGATGT GGGAGCGAGG AGACAAGACC AATCAAGGCA TTCCTGAACT GAATGGCAGC 
TCCGTCGGAA TGGCAAAGGC AGCTTTGGAG GCCATAGATG AGCTTGATTT GTTTGGAGCT 
CATGGAGGTC CAAAATCAGT GATTCACGTT CTCCCAGATG AAGTAGAACA TTGTCAGTCT 
ATCTTGTGCT CCATGCTTCC TCGAGCCTCT ACCTCTAAAG AGATTGATGC TGGTCTGCTC 
TCTGTCATCT CATTTCCTGC CTTTGCTGTG GAGGATGCGG ATCTGGTCAA CATCACCAAG 
GCCGAAATCA TCACCAAGCT GCAGGGACGT TATGGCTGTT GTCGATTTAT CCGAGATGGA 
TACAGGACTC CAAAAGAGGA CCCTACTCGA CTTCACTATG ACCCTGCCGA GCTGAAGCTC 
TTTGAGAACA TTGAATGTGA ATGGCCAGTG TTTTGGACTT ATCTAATACT CGATGGCATC 
TTCAATGCAG ATCATGAACA GGTGGAGGAA TACAGAGAGG CTCTGGATGG GGTTTTGATC 
AGGGGCAAAC ATGGTATCCG TCTGGTGCCT GAACTCTACG CTGTACCTGC TGACAAAGTT 
GAGGAGGAGT ACAGGAACCC TCATTCGGTG GAGCGTGTGG CTTTTGGGCA GCTTCCACAC 
ATGTGGGGTC AGTCTCTGTA TATACTGGGC AGTCTCCTGA CTGAGGGATT TCTGGCCCCT 
GGTGAAATTG ATCCACTTAA TAGGCGGTTT TCTAAAGAAT TCAAGCCTGA TGTTGTTGTG 
CAAGTGAGTG TAGTGGCTGA GTCAGTGCAG ATCCAGCAGC TGCTGAGGGA TCATGGGATT 
GAGGTTCAGA CCGTCTCTGA TGTATCGCCC ATCCGGGTTA TGCCAGCTCG CATCCTGAGC 
CACATTTATG TGAAACTGGG AAACTGTAAA AAACTCAACT TGAGTGGACG ACCGTACAGG 
CACATTGGCG TTCTGGGAAC TTCAAAGTTT TATGAGATCA GAAATGGCAC TTACACTTTT 
ACTCCACAGT TCATTGACCA GCATCACTTC TACTTGGCTC TGGATAACCA GATGATTGTT 
GAGATGTTGC GGACAGAACT TGCTTACCTC TCGTCCTGCT GGCGAATGAC AGGAAGACCA 
ACCCTCACAT TCCCTATTAC ACAGAGCATG CTGGTTGAAG ATGGTGACAG TATTGACCCC 
TGCATCCTCT CCACCTTGCG GAAGCTTCAG GATGGCTACT TTGGTGGTGC AAGGGTGCAG 
ATGGCAAACC TGTCCTCGTT CCTCACCACC TCCTTCCACA CGCAGCTCAG CTTTTTGGAC 
GGCGACTCTG AAGAGGATCT GCTGGAGGAG GAAGAGGAGG AAGATGAAGA GGCTTCCGCA 
GGAGGATCAG GAGACATGTT CGACCAGTAC CTCACGGATT TATTGCACAG CACTACCACA 
AAATGCCATC TACCTCCCAT CCAAAGAGGG CAGCACCACG TTTTCAGTGC TGAACACACC 
ACCAGAGACA TACTGTCCTT CATGGCCCAA GTTCAAGGCC ACAACATGCC AAAGCCGTCC 
ATGTATTTGC CTGTTGCCCC TATCATGAGC AAACACAGGA AGTCTCTGAA CCTGCTAGAT 
GTCCCTCAAC ATAAAGCTTC ACCTCAGACA AAGGCTGATA ACGTTGACCT GCATTTACCA 
CGCGATTCTC ATGGAAACAC AGACTTTGTT CATCTGGTGG ATCAGCTTAA GCACTGTCCA 
ACCCTGCAGG ACCAGGCCGA TATCCTCTAT GTCCTCTATG CTATGAAAGG CCCGAACTGG 
GTGGTGAGTT TGTCAGGACA GGGTGAGGCG ACAGTTCGCT CTCTGCTGGA GGAGCTGTAC 
GTTCGGGCAG GAGCGTGTAA GGAATGGGGC CTCATCCGCT ACATCTCCGG CATCCTGAAG 
AAGAGGGTGG AAGTCCTTGC TGAAGCCTGC ACAGATCTGA TCTCTCATCA TAAACAGCTG 
ACAGTGGGTC TTCCTCCTGA ACCCAGAGAG AAAGTCATCA CGGTTCCTCT TCCTCCTGAA 
GAATTGATCA GCTTGATCTA CGAGGCCAGC GGACAAGACA TCAGCATTGC AGTCCTCACA 
CAGGAGATTA TGGTGTATTT GGCCATGTAC GTGCGCTCTC AGCCATCTCT GTTTGGAGAT 
ATGCTGCGTC TGCGCATCGG TCTCATCATG CAGGTGATGG CAACTGAACT GGCCCGCAGT 
CTGCACTGCT CAGGAGAAGA GGCTTCAGAG AGCCTGATGA GCCTCAGTCC TTTTGACATG 
AAGAATCTCT TACATCACAT CCTGAGCGGG AAAGAGTTTG GCGTTGAGAG GAGCATGCGT 
CCAATTCAGT CGTCTGCCAC CAGTCCTGCC ATCTCCATCC ATGAGCTCGG ACACACAGGA 
GCCACCAAGA CAGAGCGGAC AGGCATCAGA AAACTCAAGA CTGAAATTAA ACAGCTCGAT 
GACTCCAGAC CCATAAGCCG ATGCAGCAGC CCATCCACTC CCAGCGGGAT TTTGTCTCCG 
ACCGGGACAG GAGACTGTCA CCTGCAGTGG GAGGAGCGGC AGGGCCAGTG GCTGAGGAGA 
CGCAGGTTGG ACGGGGCCAT CAACCGGGTC CCCATGGGCT TCTATCAGAA AGTCTGGAAG 
ATTCTCCAAA AGTGTCATGG CCTCTCCATC GCTGGTTATG TCCTGCCTTC TTCTACCACC 
CGTGAGATGA CGGAGGGAGA GATCAAGTTT GCGGTGCATG TGGAGTCTGT CCTGAATCAT 
GTTCCTCAGC CGGAGTACAG GCAGCTGCTG GTGGAGGCTA TTATGGTGCT CACTCTTGTG 
GCCGACATGG AGATTCCTAG TGTTGGTGGA ATTATTCACA TCGACCGCAT CGTTCACATG 
GCCAACGATC TCTTTCAGCA GGATCAGAGA TCCAGCGGGG CCAATGAGTA TTTCTTGGAG 
CAAGATCCTG CCACAGGAAT CTGTAACTTC TTCTATGACA GCGCTCCCAG CGGCAGCTAT 
GGCACCATGA CTTATCTTTC CAAGGCGGTG GTCACATATG TTCAAGACTT CCTGCCAAAC 
AGCAGCTGTC TAATGCAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phka2 ( XM_009306167.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304442.1 Danio rerio phosphorylase kinase, alpha 2 (liver) (phka2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa42321
Clone ID Related Accession (Same CDS sequence)	XM_009306167.2 , XM_009306167.3
Accession Version	XM_009306167.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306167.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCC GCAGTAATTC AGGAGTGAGG CTGGACGGAT TTGCCAGACT TGTCCATGAA 
ACAATATTAT GCCACCAGAA TCCAGTGACG GGTCTACTGC CGGCCAGCGA GCAGCAGAAG 
GATGCCTGGG TCAGGGATAA CGTGTACAGC ATTTTGGCAG TGTGGGGACT TGGGATGGCT 
TACCGCAAGA ATGCAGACCG TGATGAGGAT AAAGCCAAAG CCTATGAGCT TGAACAGAGT 
GTGGTTAAGC TAATGCAAGG TCTGCTGCAG TGTATGATGA GACAGGTGGA GAAGGTAGAG 
AAGTTCAAAC ACACACAGAG CACAAAGGAC TGCCTGCATG CCAAATATCA CACACCTACC 
TGTGCCACAG TGGTCGGAGA TGACCAATGG GGGCACCTGC AGGTCGATGC CACCTCACTC 
TACCTGCTCA CACTGGCCCA GATGACTGCC TCAGGTCTGC ACATCATATC AAACTTAGAC 
GAGGTGGCTT TCATCCAGAA CCTGGTGTTC TACATCGAAG CAGCGTACAA AGTGGCTGAT 
TATGGGATGT GGGAGCGAGG AGACAAGACC AATCAAGGCA TTCCTGAACT GAATGGCAGC 
TCCGTCGGAA TGGCAAAGGC AGCTTTGGAG GCCATAGATG AGCTTGATTT GTTTGGAGCT 
CATGGAGGTC CAAAATCAGT GATTCACGTT CTCCCAGATG AAGTAGAACA TTGTCAGTCT 
ATCTTGTGCT CCATGCTTCC TCGAGCCTCT ACCTCTAAAG AGATTGATGC TGGTCTGCTC 
TCTGTCATCT CATTTCCTGC CTTTGCTGTG GAGGATGCGG ATCTGGTCAA CATCACCAAG 
GCCGAAATCA TCACCAAGCT GCAGGGACGT TATGGCTGTT GTCGATTTAT CCGAGATGGA 
TACAGGACTC CAAAAGAGGA CCCTACTCGA CTTCACTATG ACCCTGCCGA GCTGAAGCTC 
TTTGAGAACA TTGAATGTGA ATGGCCAGTG TTTTGGACTT ATCTAATACT CGATGGCATC 
TTCAATGCAG ATCATGAACA GGTGGAGGAA TACAGAGAGG CTCTGGATGG GGTTTTGATC 
AGGGGCAAAC ATGGTATCCG TCTGGTGCCT GAACTCTACG CTGTACCTGC TGACAAAGTT 
GAGGAGGAGT ACAGGAACCC TCATTCGGTG GAGCGTGTGG CTTTTGGGCA GCTTCCACAC 
ATGTGGGGTC AGTCTCTGTA TATACTGGGC AGTCTCCTGA CTGAGGGATT TCTGGCCCCT 
GGTGAAATTG ATCCACTTAA TAGGCGGTTT TCTAAAGAAT TCAAGCCTGA TGTTGTTGTG 
CAAGTGAGTG TAGTGGCTGA GTCAGTGCAG ATCCAGCAGC TGCTGAGGGA TCATGGGATT 
GAGGTTCAGA CCGTCTCTGA TGTATCGCCC ATCCGGGTTA TGCCAGCTCG CATCCTGAGC 
CACATTTATG TGAAACTGGG AAACTGTAAA AAACTCAACT TGAGTGGACG ACCGTACAGG 
CACATTGGCG TTCTGGGAAC TTCAAAGTTT TATGAGATCA GAAATGGCAC TTACACTTTT 
ACTCCACAGT TCATTGACCA GCATCACTTC TACTTGGCTC TGGATAACCA GATGATTGTT 
GAGATGTTGC GGACAGAACT TGCTTACCTC TCGTCCTGCT GGCGAATGAC AGGAAGACCA 
ACCCTCACAT TCCCTATTAC ACAGAGCATG CTGGTTGAAG ATGGTGACAG TATTGACCCC 
TGCATCCTCT CCACCTTGCG GAAGCTTCAG GATGGCTACT TTGGTGGTGC AAGGGTGCAG 
ATGGCAAACC TGTCCTCGTT CCTCACCACC TCCTTCCACA CGCAGCTCAG CTTTTTGGAC 
GGCGACTCTG AAGAGGATCT GCTGGAGGAG GAAGAGGAGG AAGATGAAGA GGCTTCCGCA 
GGAGGATCAG GAGACATGTT CGACCAGTAC CTCACGGATT TATTGCACAG CACTACCACA 
AAATGCCATC TACCTCCCAT CCAAAGAGGG CAGCACCACG TTTTCAGTGC TGAACACACC 
ACCAGAGACA TACTGTCCTT CATGGCCCAA GTTCAAGGCC ACAACATGCC AAAGCCGTCC 
ATGTATTTGC CTGTTGCCCC TATCATGAGC AAACACAGGA AGTCTCTGAA CCTGCTAGAT 
GTCCCTCAAC ATAAAGCTTC ACCTCAGACA AAGGCTGATA ACGTTGACCT GCATTTACCA 
CGCGATTCTC ATGGAAACAC AGACTTTGTT CATCTGGTGG ATCAGCTTAA GCACTGTCCA 
ACCCTGCAGG ACCAGGCCGA TATCCTCTAT GTCCTCTATG CTATGAAAGG CCCGAACTGG 
GTGGTGAGTT TGTCAGGACA GGGTGAGGCG ACAGTTCGCT CTCTGCTGGA GGAGCTGTAC 
GTTCGGGCAG GAGCGTGTAA GGAATGGGGC CTCATCCGCT ACATCTCCGG CATCCTGAAG 
AAGAGGGTGG AAGTCCTTGC TGAAGCCTGC ACAGATCTGA TCTCTCATCA TAAACAGCTG 
ACAGTGGGTC TTCCTCCTGA ACCCAGAGAG AAAGTCATCA CGGTTCCTCT TCCTCCTGAA 
GAATTGATCA GCTTGATCTA CGAGGCCAGC GGACAAGACA TCAGCATTGC AGTCCTCACA 
CAGGAGATTA TGGTGTATTT GGCCATGTAC GTGCGCTCTC AGCCATCTCT GTTTGGAGAT 
ATGCTGCGTC TGCGCATCGG TCTCATCATG CAGGTGATGG CAACTGAACT GGCCCGCAGT 
CTGCACTGCT CAGGAGAAGA GGCTTCAGAG AGCCTGATGA GCCTCAGTCC TTTTGACATG 
AAGAATCTCT TACATCACAT CCTGAGCGGG AAAGAGTTTG GCGTTGAGAG GAGCATGCGT 
CCAATTCAGT CGTCTGCCAC CAGTCCTGCC ATCTCCATCC ATGAGCTCGG ACACACAGGA 
GCCACCAAGA CAGAGCGGAC AGGCATCAGA AAACTCAAGA CTGAAATTAA ACAGATCTAT 
TCCAGCAGTC ATTCCATCAG CAGTAATGTC ACATCACCTC GCTCCACGCG ATGCAGCAGC 
CCATCCACTC CCAGCGGGAT TTTGTCTCCG ACCGGGACAG GAGACTGTCA CCTGCAGTGG 
GAGGAGCGGC AGGGCCAGTG GCTGAGGAGA CGCAGGTTGG ACGGGGCCAT CAACCGGGTC 
CCCATGGGCT TCTATCAGAA AGTCTGGAAG ATTCTCCAAA AGTGTCATGG CCTCTCCATC 
GCTGGTTATG TCCTGCCTTC TTCTACCACC CGTGAGATGA CGGAGGGAGA GATCAAGTTT 
GCGGTGCATG TGGAGTCTGT CCTGAATCAT GTTCCTCAGC CGGAGTACAG GCAGCTGCTG 
GTGGAGGCTA TTATGGTGCT CACTCTTGTG GCCGACATGG AGATTCCTAG TGTTGGTGGA 
ATTATTCACA TCGACCGCAT CGTTCACATG GCCAACGATC TCTTTCAGCA GGATCAGAGA 
TCCAGCGGGG CCAATGAGTA TTTCTTGGAG CAAGATCCTG CCACAGGAAT CTGTAACTTC 
TTCTATGACA GCGCTCCCAG CGGCAGCTAT GGCACCATGA CTTATCTTTC CAAGGCGGTG 
GTCACATATG TTCAAGACTT CCTGCCAAAC AGCAGCTGTC TAATGCAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304442.1
CDS	568..4218
Translation	MRSRSNSGVRLDGFARLVHETILCHQNPVTGLLPASEQQKDAWVRDNVYSILAVWGLGMAYRKNADRDEDKAKAYELEQSVVKLMQGLLQCMMRQVEKVEKFKHTQSTKDCLHAKYHTPTCATVVGDDQWGHLQVDATSLYLLTLAQMTASGLHIISNLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNGSSVGMAKAALEAIDELDLFGAHGGPKSVIHVLPDEVEHCQSILCSMLPRASTSKEIDAGLLSVISFPAFAVEDADLVNITKAEIITKLQGRYGCCRFIRDGYRTPKEDPTRLHYDPAELKLFENIECEWPVFWTYLILDGIFNADHEQVEEYREALDGVLIRGKHGIRLVPELYAVPADKVEEEYRNPHSVERVAFGQLPHMWGQSLYILGSLLTEGFLAPGEIDPLNRRFSKEFKPDVVVQVSVVAESVQIQQLLRDHGIEVQTVSDVSPIRVMPARILSHIYVKLGNCKKLNLSGRPYRHIGVLGTSKFYEIRNGTYTFTPQFIDQHHFYLALDNQMIVEMLRTELAYLSSCWRMTGRPTLTFPITQSMLVEDGDSIDPCILSTLRKLQDGYFGGARVQMANLSSFLTTSFHTQLSFLDGDSEEDLLEEEEEEDEEASAGGSGDMFDQYLTDLLHSTTTKCHLPPIQRGQHHVFSAEHTTRDILSFMAQVQGHNMPKPSMYLPVAPIMSKHRKSLNLLDVPQHKASPQTKADNVDLHLPRDSHGNTDFVHLVDQLKHCPTLQDQADILYVLYAMKGPNWVVSLSGQGEATVRSLLEELYVRAGACKEWGLIRYISGILKKRVEVLAEACTDLISHHKQLTVGLPPEPREKVITVPLPPEELISLIYEASGQDISIAVLTQEIMVYLAMYVRSQPSLFGDMLRLRIGLIMQVMATELARSLHCSGEEASESLMSLSPFDMKNLLHHILSGKEFGVERSMRPIQSSATSPAISIHELGHTGATKTERTGIRKLKTEIKQIYSSSHSISSNVTSPRSTRCSSPSTPSGILSPTGTGDCHLQWEERQGQWLRRRRLDGAINRVPMGFYQKVWKILQKCHGLSIAGYVLPSSTTREMTEGEIKFAVHVESVLNHVPQPEYRQLLVEAIMVLTLVADMEIPSVGGIIHIDRIVHMANDLFQQDQRSSGANEYFLEQDPATGICNFFYDSAPSGSYGTMTYLSKAVVTYVQDFLPNSSCLMQ

Target ORF information:

RefSeq Version	XM_009306167.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio phosphorylase kinase, alpha 2 (liver) (phka2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306167.2

ORF Insert Sequence:

ATGAGGAGCC GCAGTAATTC AGGAGTGAGG CTGGACGGAT TTGCCAGACT TGTCCATGAA 
ACAATATTAT GCCACCAGAA TCCAGTGACG GGTCTACTGC CGGCCAGCGA GCAGCAGAAG 
GATGCCTGGG TCAGGGATAA CGTGTACAGC ATTTTGGCAG TGTGGGGACT TGGGATGGCT 
TACCGCAAGA ATGCAGACCG TGATGAGGAT AAAGCCAAAG CCTATGAGCT TGAACAGAGT 
GTGGTTAAGC TAATGCAAGG TCTGCTGCAG TGTATGATGA GACAGGTGGA GAAGGTAGAG 
AAGTTCAAAC ACACACAGAG CACAAAGGAC TGCCTGCATG CCAAATATCA CACACCTACC 
TGTGCCACAG TGGTCGGAGA TGACCAATGG GGGCACCTGC AGGTCGATGC CACCTCACTC 
TACCTGCTCA CACTGGCCCA GATGACTGCC TCAGGTCTGC ACATCATATC AAACTTAGAC 
GAGGTGGCTT TCATCCAGAA CCTGGTGTTC TACATCGAAG CAGCGTACAA AGTGGCTGAT 
TATGGGATGT GGGAGCGAGG AGACAAGACC AATCAAGGCA TTCCTGAACT GAATGGCAGC 
TCCGTCGGAA TGGCAAAGGC AGCTTTGGAG GCCATAGATG AGCTTGATTT GTTTGGAGCT 
CATGGAGGTC CAAAATCAGT GATTCACGTT CTCCCAGATG AAGTAGAACA TTGTCAGTCT 
ATCTTGTGCT CCATGCTTCC TCGAGCCTCT ACCTCTAAAG AGATTGATGC TGGTCTGCTC 
TCTGTCATCT CATTTCCTGC CTTTGCTGTG GAGGATGCGG ATCTGGTCAA CATCACCAAG 
GCCGAAATCA TCACCAAGCT GCAGGGACGT TATGGCTGTT GTCGATTTAT CCGAGATGGA 
TACAGGACTC CAAAAGAGGA CCCTACTCGA CTTCACTATG ACCCTGCCGA GCTGAAGCTC 
TTTGAGAACA TTGAATGTGA ATGGCCAGTG TTTTGGACTT ATCTAATACT CGATGGCATC 
TTCAATGCAG ATCATGAACA GGTGGAGGAA TACAGAGAGG CTCTGGATGG GGTTTTGATC 
AGGGGCAAAC ATGGTATCCG TCTGGTGCCT GAACTCTACG CTGTACCTGC TGACAAAGTT 
GAGGAGGAGT ACAGGAACCC TCATTCGGTG GAGCGTGTGG CTTTTGGGCA GCTTCCACAC 
ATGTGGGGTC AGTCTCTGTA TATACTGGGC AGTCTCCTGA CTGAGGGATT TCTGGCCCCT 
GGTGAAATTG ATCCACTTAA TAGGCGGTTT TCTAAAGAAT TCAAGCCTGA TGTTGTTGTG 
CAAGTGAGTG TAGTGGCTGA GTCAGTGCAG ATCCAGCAGC TGCTGAGGGA TCATGGGATT 
GAGGTTCAGA CCGTCTCTGA TGTATCGCCC ATCCGGGTTA TGCCAGCTCG CATCCTGAGC 
CACATTTATG TGAAACTGGG AAACTGTAAA AAACTCAACT TGAGTGGACG ACCGTACAGG 
CACATTGGCG TTCTGGGAAC TTCAAAGTTT TATGAGATCA GAAATGGCAC TTACACTTTT 
ACTCCACAGT TCATTGACCA GCATCACTTC TACTTGGCTC TGGATAACCA GATGATTGTT 
GAGATGTTGC GGACAGAACT TGCTTACCTC TCGTCCTGCT GGCGAATGAC AGGAAGACCA 
ACCCTCACAT TCCCTATTAC ACAGAGCATG CTGGTTGAAG ATGGTGACAG TATTGACCCC 
TGCATCCTCT CCACCTTGCG GAAGCTTCAG GATGGCTACT TTGGTGGTGC AAGGGTGCAG 
ATGGCAAACC TGTCCTCGTT CCTCACCACC TCCTTCCACA CGCAGCTCAG CTTTTTGGAC 
GGCGACTCTG AAGAGGATCT GCTGGAGGAG GAAGAGGAGG AAGATGAAGA GGCTTCCGCA 
GGAGGATCAG GAGACATGTT CGACCAGTAC CTCACGGATT TATTGCACAG CACTACCACA 
AAATGCCATC TACCTCCCAT CCAAAGAGGG CAGCACCACG TTTTCAGTGC TGAACACACC 
ACCAGAGACA TACTGTCCTT CATGGCCCAA GTTCAAGGCC ACAACATGCC AAAGCCGTCC 
ATGTATTTGC CTGTTGCCCC TATCATGAGC AAACACAGGA AGTCTCTGAA CCTGCTAGAT 
GTCCCTCAAC ATAAAGCTTC ACCTCAGACA AAGGCTGATA ACGTTGACCT GCATTTACCA 
CGCGATTCTC ATGGAAACAC AGACTTTGTT CATCTGGTGG ATCAGCTTAA GCACTGTCCA 
ACCCTGCAGG ACCAGGCCGA TATCCTCTAT GTCCTCTATG CTATGAAAGG CCCGAACTGG 
GTGGTGAGTT TGTCAGGACA GGGTGAGGCG ACAGTTCGCT CTCTGCTGGA GGAGCTGTAC 
GTTCGGGCAG GAGCGTGTAA GGAATGGGGC CTCATCCGCT ACATCTCCGG CATCCTGAAG 
AAGAGGGTGG AAGTCCTTGC TGAAGCCTGC ACAGATCTGA TCTCTCATCA TAAACAGCTG 
ACAGTGGGTC TTCCTCCTGA ACCCAGAGAG AAAGTCATCA CGGTTCCTCT TCCTCCTGAA 
GAATTGATCA GCTTGATCTA CGAGGCCAGC GGACAAGACA TCAGCATTGC AGTCCTCACA 
CAGGAGATTA TGGTGTATTT GGCCATGTAC GTGCGCTCTC AGCCATCTCT GTTTGGAGAT 
ATGCTGCGTC TGCGCATCGG TCTCATCATG CAGGTGATGG CAACTGAACT GGCCCGCAGT 
CTGCACTGCT CAGGAGAAGA GGCTTCAGAG AGCCTGATGA GCCTCAGTCC TTTTGACATG 
AAGAATCTCT TACATCACAT CCTGAGCGGG AAAGAGTTTG GCGTTGAGAG GAGCATGCGT 
CCAATTCAGT CGTCTGCCAC CAGTCCTGCC ATCTCCATCC ATGAGCTCGG ACACACAGGA 
GCCACCAAGA CAGAGCGGAC AGGCATCAGA AAACTCAAGA CTGAAATTAA ACAGATCTAT 
TCCAGCAGTC ATTCCATCAG CAGTAATGTC ACATCACCTC GCTCCACGCG ATGCAGCAGC 
CCATCCACTC CCAGCGGGAT TTTGTCTCCG ACCGGGACAG GAGACTGTCA CCTGCAGTGG 
GAGGAGCGGC AGGGCCAGTG GCTGAGGAGA CGCAGGTTGG ACGGGGCCAT CAACCGGGTC 
CCCATGGGCT TCTATCAGAA AGTCTGGAAG ATTCTCCAAA AGTGTCATGG CCTCTCCATC 
GCTGGTTATG TCCTGCCTTC TTCTACCACC CGTGAGATGA CGGAGGGAGA GATCAAGTTT 
GCGGTGCATG TGGAGTCTGT CCTGAATCAT GTTCCTCAGC CGGAGTACAG GCAGCTGCTG 
GTGGAGGCTA TTATGGTGCT CACTCTTGTG GCCGACATGG AGATTCCTAG TGTTGGTGGA 
ATTATTCACA TCGACCGCAT CGTTCACATG GCCAACGATC TCTTTCAGCA GGATCAGAGA 
TCCAGCGGGG CCAATGAGTA TTTCTTGGAG CAAGATCCTG CCACAGGAAT CTGTAACTTC 
TTCTATGACA GCGCTCCCAG CGGCAGCTAT GGCACCATGA CTTATCTTTC CAAGGCGGTG 
GTCACATATG TTCAAGACTT CCTGCCAAAC AGCAGCTGTC TAATGCAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

phka2 ( XM_009306167.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304442.1 Danio rerio phosphorylase kinase, alpha 2 (liver) (phka2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa42321
Clone ID Related Accession (Same CDS sequence)	XM_009306167.2 , XM_009306167.3
Accession Version	XM_009306167.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3651bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306167.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCC GCAGTAATTC AGGAGTGAGG CTGGACGGAT TTGCCAGACT TGTCCATGAA 
ACAATATTAT GCCACCAGAA TCCAGTGACG GGTCTACTGC CGGCCAGCGA GCAGCAGAAG 
GATGCCTGGG TCAGGGATAA CGTGTACAGC ATTTTGGCAG TGTGGGGACT TGGGATGGCT 
TACCGCAAGA ATGCAGACCG TGATGAGGAT AAAGCCAAAG CCTATGAGCT TGAACAGAGT 
GTGGTTAAGC TAATGCAAGG TCTGCTGCAG TGTATGATGA GACAGGTGGA GAAGGTAGAG 
AAGTTCAAAC ACACACAGAG CACAAAGGAC TGCCTGCATG CCAAATATCA CACACCTACC 
TGTGCCACAG TGGTCGGAGA TGACCAATGG GGGCACCTGC AGGTCGATGC CACCTCACTC 
TACCTGCTCA CACTGGCCCA GATGACTGCC TCAGGTCTGC ACATCATATC AAACTTAGAC 
GAGGTGGCTT TCATCCAGAA CCTGGTGTTC TACATCGAAG CAGCGTACAA AGTGGCTGAT 
TATGGGATGT GGGAGCGAGG AGACAAGACC AATCAAGGCA TTCCTGAACT GAATGGCAGC 
TCCGTCGGAA TGGCAAAGGC AGCTTTGGAG GCCATAGATG AGCTTGATTT GTTTGGAGCT 
CATGGAGGTC CAAAATCAGT GATTCACGTT CTCCCAGATG AAGTAGAACA TTGTCAGTCT 
ATCTTGTGCT CCATGCTTCC TCGAGCCTCT ACCTCTAAAG AGATTGATGC TGGTCTGCTC 
TCTGTCATCT CATTTCCTGC CTTTGCTGTG GAGGATGCGG ATCTGGTCAA CATCACCAAG 
GCCGAAATCA TCACCAAGCT GCAGGGACGT TATGGCTGTT GTCGATTTAT CCGAGATGGA 
TACAGGACTC CAAAAGAGGA CCCTACTCGA CTTCACTATG ACCCTGCCGA GCTGAAGCTC 
TTTGAGAACA TTGAATGTGA ATGGCCAGTG TTTTGGACTT ATCTAATACT CGATGGCATC 
TTCAATGCAG ATCATGAACA GGTGGAGGAA TACAGAGAGG CTCTGGATGG GGTTTTGATC 
AGGGGCAAAC ATGGTATCCG TCTGGTGCCT GAACTCTACG CTGTACCTGC TGACAAAGTT 
GAGGAGGAGT ACAGGAACCC TCATTCGGTG GAGCGTGTGG CTTTTGGGCA GCTTCCACAC 
ATGTGGGGTC AGTCTCTGTA TATACTGGGC AGTCTCCTGA CTGAGGGATT TCTGGCCCCT 
GGTGAAATTG ATCCACTTAA TAGGCGGTTT TCTAAAGAAT TCAAGCCTGA TGTTGTTGTG 
CAAGTGAGTG TAGTGGCTGA GTCAGTGCAG ATCCAGCAGC TGCTGAGGGA TCATGGGATT 
GAGGTTCAGA CCGTCTCTGA TGTATCGCCC ATCCGGGTTA TGCCAGCTCG CATCCTGAGC 
CACATTTATG TGAAACTGGG AAACTGTAAA AAACTCAACT TGAGTGGACG ACCGTACAGG 
CACATTGGCG TTCTGGGAAC TTCAAAGTTT TATGAGATCA GAAATGGCAC TTACACTTTT 
ACTCCACAGT TCATTGACCA GCATCACTTC TACTTGGCTC TGGATAACCA GATGATTGTT 
GAGATGTTGC GGACAGAACT TGCTTACCTC TCGTCCTGCT GGCGAATGAC AGGAAGACCA 
ACCCTCACAT TCCCTATTAC ACAGAGCATG CTGGTTGAAG ATGGTGACAG TATTGACCCC 
TGCATCCTCT CCACCTTGCG GAAGCTTCAG GATGGCTACT TTGGTGGTGC AAGGGTGCAG 
ATGGCAAACC TGTCCTCGTT CCTCACCACC TCCTTCCACA CGCAGCTCAG CTTTTTGGAC 
GGCGACTCTG AAGAGGATCT GCTGGAGGAG GAAGAGGAGG AAGATGAAGA GGCTTCCGCA 
GGAGGATCAG GAGACATGTT CGACCAGTAC CTCACGGATT TATTGCACAG CACTACCACA 
AAATGCCATC TACCTCCCAT CCAAAGAGGG CAGCACCACG TTTTCAGTGC TGAACACACC 
ACCAGAGACA TACTGTCCTT CATGGCCCAA GTTCAAGGCC ACAACATGCC AAAGCCGTCC 
ATGTATTTGC CTGTTGCCCC TATCATGAGC AAACACAGGA AGTCTCTGAA CCTGCTAGAT 
GTCCCTCAAC ATAAAGCTTC ACCTCAGACA AAGGCTGATA ACGTTGACCT GCATTTACCA 
CGCGATTCTC ATGGAAACAC AGACTTTGTT CATCTGGTGG ATCAGCTTAA GCACTGTCCA 
ACCCTGCAGG ACCAGGCCGA TATCCTCTAT GTCCTCTATG CTATGAAAGG CCCGAACTGG 
GTGGTGAGTT TGTCAGGACA GGGTGAGGCG ACAGTTCGCT CTCTGCTGGA GGAGCTGTAC 
GTTCGGGCAG GAGCGTGTAA GGAATGGGGC CTCATCCGCT ACATCTCCGG CATCCTGAAG 
AAGAGGGTGG AAGTCCTTGC TGAAGCCTGC ACAGATCTGA TCTCTCATCA TAAACAGCTG 
ACAGTGGGTC TTCCTCCTGA ACCCAGAGAG AAAGTCATCA CGGTTCCTCT TCCTCCTGAA 
GAATTGATCA GCTTGATCTA CGAGGCCAGC GGACAAGACA TCAGCATTGC AGTCCTCACA 
CAGGAGATTA TGGTGTATTT GGCCATGTAC GTGCGCTCTC AGCCATCTCT GTTTGGAGAT 
ATGCTGCGTC TGCGCATCGG TCTCATCATG CAGGTGATGG CAACTGAACT GGCCCGCAGT 
CTGCACTGCT CAGGAGAAGA GGCTTCAGAG AGCCTGATGA GCCTCAGTCC TTTTGACATG 
AAGAATCTCT TACATCACAT CCTGAGCGGG AAAGAGTTTG GCGTTGAGAG GAGCATGCGT 
CCAATTCAGT CGTCTGCCAC CAGTCCTGCC ATCTCCATCC ATGAGCTCGG ACACACAGGA 
GCCACCAAGA CAGAGCGGAC AGGCATCAGA AAACTCAAGA CTGAAATTAA ACAGATCTAT 
TCCAGCAGTC ATTCCATCAG CAGTAATGTC ACATCACCTC GCTCCACGCG ATGCAGCAGC 
CCATCCACTC CCAGCGGGAT TTTGTCTCCG ACCGGGACAG GAGACTGTCA CCTGCAGTGG 
GAGGAGCGGC AGGGCCAGTG GCTGAGGAGA CGCAGGTTGG ACGGGGCCAT CAACCGGGTC 
CCCATGGGCT TCTATCAGAA AGTCTGGAAG ATTCTCCAAA AGTGTCATGG CCTCTCCATC 
GCTGGTTATG TCCTGCCTTC TTCTACCACC CGTGAGATGA CGGAGGGAGA GATCAAGTTT 
GCGGTGCATG TGGAGTCTGT CCTGAATCAT GTTCCTCAGC CGGAGTACAG GCAGCTGCTG 
GTGGAGGCTA TTATGGTGCT CACTCTTGTG GCCGACATGG AGATTCCTAG TGTTGGTGGA 
ATTATTCACA TCGACCGCAT CGTTCACATG GCCAACGATC TCTTTCAGCA GGATCAGAGA 
TCCAGCGGGG CCAATGAGTA TTTCTTGGAG CAAGATCCTG CCACAGGAAT CTGTAACTTC 
TTCTATGACA GCGCTCCCAG CGGCAGCTAT GGCACCATGA CTTATCTTTC CAAGGCGGTG 
GTCACATATG TTCAAGACTT CCTGCCAAAC AGCAGCTGTC TAATGCAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304442.1
CDS	580..4230
Translation	MRSRSNSGVRLDGFARLVHETILCHQNPVTGLLPASEQQKDAWVRDNVYSILAVWGLGMAYRKNADRDEDKAKAYELEQSVVKLMQGLLQCMMRQVEKVEKFKHTQSTKDCLHAKYHTPTCATVVGDDQWGHLQVDATSLYLLTLAQMTASGLHIISNLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNGSSVGMAKAALEAIDELDLFGAHGGPKSVIHVLPDEVEHCQSILCSMLPRASTSKEIDAGLLSVISFPAFAVEDADLVNITKAEIITKLQGRYGCCRFIRDGYRTPKEDPTRLHYDPAELKLFENIECEWPVFWTYLILDGIFNADHEQVEEYREALDGVLIRGKHGIRLVPELYAVPADKVEEEYRNPHSVERVAFGQLPHMWGQSLYILGSLLTEGFLAPGEIDPLNRRFSKEFKPDVVVQVSVVAESVQIQQLLRDHGIEVQTVSDVSPIRVMPARILSHIYVKLGNCKKLNLSGRPYRHIGVLGTSKFYEIRNGTYTFTPQFIDQHHFYLALDNQMIVEMLRTELAYLSSCWRMTGRPTLTFPITQSMLVEDGDSIDPCILSTLRKLQDGYFGGARVQMANLSSFLTTSFHTQLSFLDGDSEEDLLEEEEEEDEEASAGGSGDMFDQYLTDLLHSTTTKCHLPPIQRGQHHVFSAEHTTRDILSFMAQVQGHNMPKPSMYLPVAPIMSKHRKSLNLLDVPQHKASPQTKADNVDLHLPRDSHGNTDFVHLVDQLKHCPTLQDQADILYVLYAMKGPNWVVSLSGQGEATVRSLLEELYVRAGACKEWGLIRYISGILKKRVEVLAEACTDLISHHKQLTVGLPPEPREKVITVPLPPEELISLIYEASGQDISIAVLTQEIMVYLAMYVRSQPSLFGDMLRLRIGLIMQVMATELARSLHCSGEEASESLMSLSPFDMKNLLHHILSGKEFGVERSMRPIQSSATSPAISIHELGHTGATKTERTGIRKLKTEIKQIYSSSHSISSNVTSPRSTRCSSPSTPSGILSPTGTGDCHLQWEERQGQWLRRRRLDGAINRVPMGFYQKVWKILQKCHGLSIAGYVLPSSTTREMTEGEIKFAVHVESVLNHVPQPEYRQLLVEAIMVLTLVADMEIPSVGGIIHIDRIVHMANDLFQQDQRSSGANEYFLEQDPATGICNFFYDSAPSGSYGTMTYLSKAVVTYVQDFLPNSSCLMQ

Target ORF information:

RefSeq Version	XM_009306167.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio phosphorylase kinase, alpha 2 (liver) (phka2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306167.3

ORF Insert Sequence:

ATGAGGAGCC GCAGTAATTC AGGAGTGAGG CTGGACGGAT TTGCCAGACT TGTCCATGAA 
ACAATATTAT GCCACCAGAA TCCAGTGACG GGTCTACTGC CGGCCAGCGA GCAGCAGAAG 
GATGCCTGGG TCAGGGATAA CGTGTACAGC ATTTTGGCAG TGTGGGGACT TGGGATGGCT 
TACCGCAAGA ATGCAGACCG TGATGAGGAT AAAGCCAAAG CCTATGAGCT TGAACAGAGT 
GTGGTTAAGC TAATGCAAGG TCTGCTGCAG TGTATGATGA GACAGGTGGA GAAGGTAGAG 
AAGTTCAAAC ACACACAGAG CACAAAGGAC TGCCTGCATG CCAAATATCA CACACCTACC 
TGTGCCACAG TGGTCGGAGA TGACCAATGG GGGCACCTGC AGGTCGATGC CACCTCACTC 
TACCTGCTCA CACTGGCCCA GATGACTGCC TCAGGTCTGC ACATCATATC AAACTTAGAC 
GAGGTGGCTT TCATCCAGAA CCTGGTGTTC TACATCGAAG CAGCGTACAA AGTGGCTGAT 
TATGGGATGT GGGAGCGAGG AGACAAGACC AATCAAGGCA TTCCTGAACT GAATGGCAGC 
TCCGTCGGAA TGGCAAAGGC AGCTTTGGAG GCCATAGATG AGCTTGATTT GTTTGGAGCT 
CATGGAGGTC CAAAATCAGT GATTCACGTT CTCCCAGATG AAGTAGAACA TTGTCAGTCT 
ATCTTGTGCT CCATGCTTCC TCGAGCCTCT ACCTCTAAAG AGATTGATGC TGGTCTGCTC 
TCTGTCATCT CATTTCCTGC CTTTGCTGTG GAGGATGCGG ATCTGGTCAA CATCACCAAG 
GCCGAAATCA TCACCAAGCT GCAGGGACGT TATGGCTGTT GTCGATTTAT CCGAGATGGA 
TACAGGACTC CAAAAGAGGA CCCTACTCGA CTTCACTATG ACCCTGCCGA GCTGAAGCTC 
TTTGAGAACA TTGAATGTGA ATGGCCAGTG TTTTGGACTT ATCTAATACT CGATGGCATC 
TTCAATGCAG ATCATGAACA GGTGGAGGAA TACAGAGAGG CTCTGGATGG GGTTTTGATC 
AGGGGCAAAC ATGGTATCCG TCTGGTGCCT GAACTCTACG CTGTACCTGC TGACAAAGTT 
GAGGAGGAGT ACAGGAACCC TCATTCGGTG GAGCGTGTGG CTTTTGGGCA GCTTCCACAC 
ATGTGGGGTC AGTCTCTGTA TATACTGGGC AGTCTCCTGA CTGAGGGATT TCTGGCCCCT 
GGTGAAATTG ATCCACTTAA TAGGCGGTTT TCTAAAGAAT TCAAGCCTGA TGTTGTTGTG 
CAAGTGAGTG TAGTGGCTGA GTCAGTGCAG ATCCAGCAGC TGCTGAGGGA TCATGGGATT 
GAGGTTCAGA CCGTCTCTGA TGTATCGCCC ATCCGGGTTA TGCCAGCTCG CATCCTGAGC 
CACATTTATG TGAAACTGGG AAACTGTAAA AAACTCAACT TGAGTGGACG ACCGTACAGG 
CACATTGGCG TTCTGGGAAC TTCAAAGTTT TATGAGATCA GAAATGGCAC TTACACTTTT 
ACTCCACAGT TCATTGACCA GCATCACTTC TACTTGGCTC TGGATAACCA GATGATTGTT 
GAGATGTTGC GGACAGAACT TGCTTACCTC TCGTCCTGCT GGCGAATGAC AGGAAGACCA 
ACCCTCACAT TCCCTATTAC ACAGAGCATG CTGGTTGAAG ATGGTGACAG TATTGACCCC 
TGCATCCTCT CCACCTTGCG GAAGCTTCAG GATGGCTACT TTGGTGGTGC AAGGGTGCAG 
ATGGCAAACC TGTCCTCGTT CCTCACCACC TCCTTCCACA CGCAGCTCAG CTTTTTGGAC 
GGCGACTCTG AAGAGGATCT GCTGGAGGAG GAAGAGGAGG AAGATGAAGA GGCTTCCGCA 
GGAGGATCAG GAGACATGTT CGACCAGTAC CTCACGGATT TATTGCACAG CACTACCACA 
AAATGCCATC TACCTCCCAT CCAAAGAGGG CAGCACCACG TTTTCAGTGC TGAACACACC 
ACCAGAGACA TACTGTCCTT CATGGCCCAA GTTCAAGGCC ACAACATGCC AAAGCCGTCC 
ATGTATTTGC CTGTTGCCCC TATCATGAGC AAACACAGGA AGTCTCTGAA CCTGCTAGAT 
GTCCCTCAAC ATAAAGCTTC ACCTCAGACA AAGGCTGATA ACGTTGACCT GCATTTACCA 
CGCGATTCTC ATGGAAACAC AGACTTTGTT CATCTGGTGG ATCAGCTTAA GCACTGTCCA 
ACCCTGCAGG ACCAGGCCGA TATCCTCTAT GTCCTCTATG CTATGAAAGG CCCGAACTGG 
GTGGTGAGTT TGTCAGGACA GGGTGAGGCG ACAGTTCGCT CTCTGCTGGA GGAGCTGTAC 
GTTCGGGCAG GAGCGTGTAA GGAATGGGGC CTCATCCGCT ACATCTCCGG CATCCTGAAG 
AAGAGGGTGG AAGTCCTTGC TGAAGCCTGC ACAGATCTGA TCTCTCATCA TAAACAGCTG 
ACAGTGGGTC TTCCTCCTGA ACCCAGAGAG AAAGTCATCA CGGTTCCTCT TCCTCCTGAA 
GAATTGATCA GCTTGATCTA CGAGGCCAGC GGACAAGACA TCAGCATTGC AGTCCTCACA 
CAGGAGATTA TGGTGTATTT GGCCATGTAC GTGCGCTCTC AGCCATCTCT GTTTGGAGAT 
ATGCTGCGTC TGCGCATCGG TCTCATCATG CAGGTGATGG CAACTGAACT GGCCCGCAGT 
CTGCACTGCT CAGGAGAAGA GGCTTCAGAG AGCCTGATGA GCCTCAGTCC TTTTGACATG 
AAGAATCTCT TACATCACAT CCTGAGCGGG AAAGAGTTTG GCGTTGAGAG GAGCATGCGT 
CCAATTCAGT CGTCTGCCAC CAGTCCTGCC ATCTCCATCC ATGAGCTCGG ACACACAGGA 
GCCACCAAGA CAGAGCGGAC AGGCATCAGA AAACTCAAGA CTGAAATTAA ACAGATCTAT 
TCCAGCAGTC ATTCCATCAG CAGTAATGTC ACATCACCTC GCTCCACGCG ATGCAGCAGC 
CCATCCACTC CCAGCGGGAT TTTGTCTCCG ACCGGGACAG GAGACTGTCA CCTGCAGTGG 
GAGGAGCGGC AGGGCCAGTG GCTGAGGAGA CGCAGGTTGG ACGGGGCCAT CAACCGGGTC 
CCCATGGGCT TCTATCAGAA AGTCTGGAAG ATTCTCCAAA AGTGTCATGG CCTCTCCATC 
GCTGGTTATG TCCTGCCTTC TTCTACCACC CGTGAGATGA CGGAGGGAGA GATCAAGTTT 
GCGGTGCATG TGGAGTCTGT CCTGAATCAT GTTCCTCAGC CGGAGTACAG GCAGCTGCTG 
GTGGAGGCTA TTATGGTGCT CACTCTTGTG GCCGACATGG AGATTCCTAG TGTTGGTGGA 
ATTATTCACA TCGACCGCAT CGTTCACATG GCCAACGATC TCTTTCAGCA GGATCAGAGA 
TCCAGCGGGG CCAATGAGTA TTTCTTGGAG CAAGATCCTG CCACAGGAAT CTGTAACTTC 
TTCTATGACA GCGCTCCCAG CGGCAGCTAT GGCACCATGA CTTATCTTTC CAAGGCGGTG 
GTCACATATG TTCAAGACTT CCTGCCAAAC AGCAGCTGTC TAATGCAGTA A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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phka2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

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