PHKA2 gene cDNA ORF clone, Bos taurus(cattle)

mRNA	Protein	Name
XM_010822157.2	XP_010820459.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X1
XM_005228392.3	XP_005228449.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X3
XM_005228394.3	XP_005228451.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X5
XM_024987967.1	XP_024843735.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X2
XM_024987968.1	XP_024843736.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X3
XM_024987969.1	XP_024843737.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X4
XM_005228390.3	XP_005228447.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X1
XM_005228393.3	XP_005228450.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X4
NM_001191545.1	NP_001178474.1	phosphorylase b kinase regulatory subunit alpha, liver isoform
XM_005228391.2	XP_005228448.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X2
XM_024987966.1	XP_024843734.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X1
XM_024987970.1	XP_024843738.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X5
XM_024987971.1	XP_024843739.1	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X6

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OBa238361	XM_024987967.1 Latest version!	Bos taurus phosphorylase kinase regulatory subunit alpha 2 (PHKA2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa238362	XM_024987968.1 Latest version!	Bos taurus phosphorylase kinase regulatory subunit alpha 2 (PHKA2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa238363	XM_024987969.1 Latest version!	Bos taurus phosphorylase kinase regulatory subunit alpha 2 (PHKA2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa238364	XM_024987970.1 Latest version!	Bos taurus phosphorylase kinase regulatory subunit alpha 2 (PHKA2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa238365	XM_024987971.1 Latest version!	Bos taurus phosphorylase kinase regulatory subunit alpha 2 (PHKA2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
OBa35760	NM_001191545.1 Latest version!	Bos taurus phosphorylase kinase regulatory subunit alpha 2 (PHKA2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa35759	XM_010822157.2 Latest version!	Bos taurus phosphorylase kinase, alpha 2 (liver) (PHKA2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa35759	XM_005228390.3 Latest version!	Bos taurus phosphorylase kinase, alpha 2 (liver) (PHKA2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa35760	XM_024987966.1 Latest version!	Bos taurus phosphorylase kinase regulatory subunit alpha 2 (PHKA2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa35760	XM_005228391.2 Latest version!	Bos taurus phosphorylase kinase, alpha 2 (liver) (PHKA2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa35761	XM_005228392.3 Latest version!	Bos taurus phosphorylase kinase, alpha 2 (liver) (PHKA2), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa35762	XM_005228393.3 Latest version!	Bos taurus phosphorylase kinase, alpha 2 (liver) (PHKA2), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00
OBa35763	XM_005228394.3 Latest version!	Bos taurus phosphorylase kinase, alpha 2 (liver) (PHKA2), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$713.30 $1019.00

PHKA2 ( XM_024987967.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024843735.1 Bos taurus phosphorylase kinase regulatory subunit alpha 2 (PHKA2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa238361
Clone ID Related Accession (Same CDS sequence)	XM_024987967.1
Accession Version	XM_024987967.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3654bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCA GGAGCAATTC CGGGGTCCGC TTAGACGGAT ACGCGCGGCT GGTGCAGCAA 
ACAATCCTCT GTTACCAGAA TCCCATCACG GGGCTGATGT CAGCCAGTCA TGAGCAGAAG 
GACGCCTGGG TACGGGATAA CATCTACAGC ATCCTGGCCG TGTGGGGCCT GGGCATGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAAT 
GTGGTGAAAC TGATGCGAGG TCTTCTCCAG TGCATGATGA GACAGGTGGA TAAAGTGGAG 
AGGTTCAAGC ACACTCAGAG TACCGAGGAC AGCCTGCACG CCAAGTACAA CACTGCCACC 
TGCAGCACTG TGGTGGGCGA CGACGAGTGG GGCCACCTCC AGGTGGATGC CACCTCCCTC 
TTCCTCCTGT TTCTGGCCCA GATGACAGCC TCCGGCTTAC GTATTATTTT CACTCTTGAC 
GAGGTGGCCT TCATACAGAA TCTTGTTTTT TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGAATGT GGGAACGTGG AGATAAGACT AATCAGGGTA TTCCAGAATT GAATGCAAGC 
TCTGTAGGAA TGGCCAAGGC AGCCCTTGAA GCAATTGATG AACTGGACCT TTTTGGAGCC 
CATGGAGGTC GCAAGTCTGT GATCCACGTC CTGCCGGATG AGGTGGAACA CTGCCAGTCT 
ATTCTGTTCT CCATGCTGCC AAGAGCCTCG ACGTCAAAAG AGATTGATGC TGGGCTTCTT 
TCCATTATTT CTTTCCCGGC CTTCGCGGTG GAAGATGTGA ACCTTGTGAA TGTGACAAAA 
AATGAAATTA TTTCCAAGCT CCAGGGGCGT TATGGATGCT GTCGCTTCCT TCGAGATGGT 
TATAAAACCC CAAGAGAGGA CCCAAACCGA TTGCATTATG ACCCTGCTGA ACTCAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTCATAAT AGATGGAGTC 
TTCAATGGTG ATGCCGTTCA GGTCCAAGAA TACCGTGAAG CCCTGGAGGG GATACTGATC 
AGAGGCAATA ACGGGGTCCA CTTGTTGCCT GAACTCTACG CCATCCCACC TGACAAGGTG 
GATGAAGAGT ATAAGAATCC TCACACGGTG GATAGAGTTC CCCTGGGGAA GGTGCCGCAT 
CTGTGGGGTC AGTCCTTGTA CATCCTCAGC TCCCTGTTGG CAGAGGGATT CCTTGCTGCT 
GGTGAAATTG ATCCCTTGAA CAGAAGATTT TCCACTTCAG TCAAACCTGA TGTCGTCGTA 
CAAGTGACTG TTCTGGCAGA AAACAACCAC ATTAAGGAGC TTCTGAGGAA ACATGGGGTC 
AACGTCCAGA GTATCGCTGA CATTCATCCA ATCCGAGTCC AGCCAGGCCG CATCCTTAGT 
CACATATATG CCAAGCTTGG ACGGAATAAG AATATGAGAT TGAGTGGGCG ACCGTATCGG 
CACATTGGTG TCCTTGGGAC ATCGAAACTA TACGTGATTA GGAACCAGAT CTTTACTTTC 
ACGCCCCAGT TCACCGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATCGTA 
GAGATGCTGA GGATTGAGCT GGCCTACCTG TGCACCTGCT GGCGGATGAC GGGCAGACCC 
ACGCTCACCT TCCCCATCAC GCATACCATG CTCACAAATG ATGGCTCAGA TATTCATTCC 
GCTGTTCTTG CTACAATTCG CAAACTAGAG GATGGATATT TTGGAGGAGC TAGAGTAAAA 
TTAGGGAGTC TTTCGGAATT TCTCACCACC TCTTTCTACA CGTATCTGAC CTTCCTGGAT 
CCAGACTGTG ATGAGAAGTT GTTTGATGAT GCCAGTGAAG GGACCTTCAG TCCTGATAGT 
GATTCAGACC TGGGAGGATA TTTGGAAGAC ACCTATAATC AAGAAAGCCA GGACGAGCTT 
GACCAGTATA TCAACCACCT TCTGCAAAGC ACATCCTCGA AGTGCTATCT GCCACCTCTC 
TGTAAGAAGA CAGAGGACAA CCAGGTTTTC AGTGCTATCC ATTCCACTCG GGATATACTT 
TCTGTGATGG CAAAAGCAAA GGGCTTGGAA ATTCCATTTG TTCCCATGGC TTTGCCAACT 
AAAGTTCTAA GTGCCCACCG TAAATCGCTG AATCTCGTTG ATTCTCCTCA GCCACTCCTA 
AAAAAGGCCC CTGAAGATGA CTTCCAGTTT CCCAGAGATG ACCATGGCGA CGTGGACTGT 
GAGAAGCTGG TTGAGCAGTT GAAAGACTGT TCGAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTGT ATGTAATAAA GGGTCCCAGC TGGGACACAA ATCTCTCTGG ACAGCACGGG 
GTCACTGTTC ACAACCTCCT CAGTGAGCTC TATGCGAAGG CCGGCTTGAA CCAGGAGTGG 
GGCCTGATCC GCTACATCTC CGGCCTCCTC CGGAAGAAAG TGGAGGTCCT GGCTGAGGCG 
TGCACGGACC TGCTGTCACA CCAGAAGCAG CTCACAGTGG GCCTGCCCCC AGAACCCCGG 
GAGAAGACCA TCTCTGCGCC TCTTCCCCCC GAGGAGCTCA CGAAGCTCAT CTACGAAGCC 
AGCGGGCAGG ACATCAGCAT CGCCGTGCTC ACACAGGAGA TCGTGGTGTA CCTGGCGATG 
TACGTCAGGG CACAGCCCAG CCTCTTCGTG GAGATGCTCC GGCTCCGAAT TGGACTGATC 
ATTCAGGTGA TGGCCACGGA GCTGGCACGG AGCCTGAACT GTTCAGGAGA AGAAGCTTCT 
GAAAGTCTGA TGAACCTCAG CCCTTTTGAT ATGAAAAATC TCCTGCACCA CATCTTAAGT 
GGCAAAGAAT TTGGTGTTGA AAGAAGCATG CGGCCCATTC ACTCCTCCAC ACCTAGCCCT 
GCTGTCTCCA TCCATGAGGT GGGCCACACT GGAGTCACCA AAACCGAGAG GAGCGGCATC 
AACAGGCTGA GGAGTGAGAT GAAACAGATG ACTAGGCGGT TTAGTGCTGA TGAACAGAGG 
TCCAGCACCC CATCCTCACC CACGGGCACA TCGTCATCAG ACTCGGGCAG GCAGCACATG 
GGCTGGGGGG AACGGCAGGG CCAGTGGCTG CGCAGGAGAA GGCTGGACGG CGCCATCAAC 
AGGGTCCCCG TGGGATTCTA CCAGAAGGTG TGGAAGATCC TTCAGAAGTG CCATGGTCTG 
TCCATCGACG GTTACGTGCT CCCGTCCTCG ACCACGCAAG AGATGACCCC GCAGGAGATC 
AAGTTTGCTG TCCACGTTGA GTCAGTGCTG AACCGAGTGC CACAACCCGA GTACCGGCAG 
CTGCTGGTGG AGGCCATCAT GGTGCTGACG CTGCTCTCGG ACACAGAGAT GAACAGCATC 
GGGGGCATCA TCCATGTGGA CCAGATCGTG CACATGGCCA ACCAGCTGTT CCTACAAGAC 
CAGGTGTCAA GTGGAGCCAC GGACACGCTG GAGAAAGACC AAGCCACAGG CATCTGCCAC 
TTCTTTTACG ACAGCGCTCC AAGCGGGGCT TATGGGACGA TGACCTACCT AACGAAGGCG 
GTGGCTTCTC ATTTGCAGGA ACTGCTGCCC AATTCAGGTT GCCAGATGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024843735.1
CDS	253..3906
Translation	MRSRSNSGVRLDGYARLVQQTILCYQNPITGLMSASHEQKDAWVRDNIYSILAVWGLGMAYRKNADRDEDKAKAYELEQNVVKLMRGLLQCMMRQVDKVERFKHTQSTEDSLHAKYNTATCSTVVGDDEWGHLQVDATSLFLLFLAQMTASGLRIIFTLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGMAKAALEAIDELDLFGAHGGRKSVIHVLPDEVEHCQSILFSMLPRASTSKEIDAGLLSIISFPAFAVEDVNLVNVTKNEIISKLQGRYGCCRFLRDGYKTPREDPNRLHYDPAELKLFENIECEWPVFWTYFIIDGVFNGDAVQVQEYREALEGILIRGNNGVHLLPELYAIPPDKVDEEYKNPHTVDRVPLGKVPHLWGQSLYILSSLLAEGFLAAGEIDPLNRRFSTSVKPDVVVQVTVLAENNHIKELLRKHGVNVQSIADIHPIRVQPGRILSHIYAKLGRNKNMRLSGRPYRHIGVLGTSKLYVIRNQIFTFTPQFTDQHHFYLALDNEMIVEMLRIELAYLCTCWRMTGRPTLTFPITHTMLTNDGSDIHSAVLATIRKLEDGYFGGARVKLGSLSEFLTTSFYTYLTFLDPDCDEKLFDDASEGTFSPDSDSDLGGYLEDTYNQESQDELDQYINHLLQSTSSKCYLPPLCKKTEDNQVFSAIHSTRDILSVMAKAKGLEIPFVPMALPTKVLSAHRKSLNLVDSPQPLLKKAPEDDFQFPRDDHGDVDCEKLVEQLKDCSNLQDQADILYILYVIKGPSWDTNLSGQHGVTVHNLLSELYAKAGLNQEWGLIRYISGLLRKKVEVLAEACTDLLSHQKQLTVGLPPEPREKTISAPLPPEELTKLIYEASGQDISIAVLTQEIVVYLAMYVRAQPSLFVEMLRLRIGLIIQVMATELARSLNCSGEEASESLMNLSPFDMKNLLHHILSGKEFGVERSMRPIHSSTPSPAVSIHEVGHTGVTKTERSGINRLRSEMKQMTRRFSADEQRSSTPSSPTGTSSSDSGRQHMGWGERQGQWLRRRRLDGAINRVPVGFYQKVWKILQKCHGLSIDGYVLPSSTTQEMTPQEIKFAVHVESVLNRVPQPEYRQLLVEAIMVLTLLSDTEMNSIGGIIHVDQIVHMANQLFLQDQVSSGATDTLEKDQATGICHFFYDSAPSGAYGTMTYLTKAVASHLQELLPNSGCQMQ

Target ORF information:

RefSeq Version	XM_024987967.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphorylase kinase regulatory subunit alpha 2 (PHKA2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024987967.1

ORF Insert Sequence:

ATGAGGAGCA GGAGCAATTC CGGGGTCCGC TTAGACGGAT ACGCGCGGCT GGTGCAGCAA 
ACAATCCTCT GTTACCAGAA TCCCATCACG GGGCTGATGT CAGCCAGTCA TGAGCAGAAG 
GACGCCTGGG TACGGGATAA CATCTACAGC ATCCTGGCCG TGTGGGGCCT GGGCATGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAAT 
GTGGTGAAAC TGATGCGAGG TCTTCTCCAG TGCATGATGA GACAGGTGGA TAAAGTGGAG 
AGGTTCAAGC ACACTCAGAG TACCGAGGAC AGCCTGCACG CCAAGTACAA CACTGCCACC 
TGCAGCACTG TGGTGGGCGA CGACGAGTGG GGCCACCTCC AGGTGGATGC CACCTCCCTC 
TTCCTCCTGT TTCTGGCCCA GATGACAGCC TCCGGCTTAC GTATTATTTT CACTCTTGAC 
GAGGTGGCCT TCATACAGAA TCTTGTTTTT TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGAATGT GGGAACGTGG AGATAAGACT AATCAGGGTA TTCCAGAATT GAATGCAAGC 
TCTGTAGGAA TGGCCAAGGC AGCCCTTGAA GCAATTGATG AACTGGACCT TTTTGGAGCC 
CATGGAGGTC GCAAGTCTGT GATCCACGTC CTGCCGGATG AGGTGGAACA CTGCCAGTCT 
ATTCTGTTCT CCATGCTGCC AAGAGCCTCG ACGTCAAAAG AGATTGATGC TGGGCTTCTT 
TCCATTATTT CTTTCCCGGC CTTCGCGGTG GAAGATGTGA ACCTTGTGAA TGTGACAAAA 
AATGAAATTA TTTCCAAGCT CCAGGGGCGT TATGGATGCT GTCGCTTCCT TCGAGATGGT 
TATAAAACCC CAAGAGAGGA CCCAAACCGA TTGCATTATG ACCCTGCTGA ACTCAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTCATAAT AGATGGAGTC 
TTCAATGGTG ATGCCGTTCA GGTCCAAGAA TACCGTGAAG CCCTGGAGGG GATACTGATC 
AGAGGCAATA ACGGGGTCCA CTTGTTGCCT GAACTCTACG CCATCCCACC TGACAAGGTG 
GATGAAGAGT ATAAGAATCC TCACACGGTG GATAGAGTTC CCCTGGGGAA GGTGCCGCAT 
CTGTGGGGTC AGTCCTTGTA CATCCTCAGC TCCCTGTTGG CAGAGGGATT CCTTGCTGCT 
GGTGAAATTG ATCCCTTGAA CAGAAGATTT TCCACTTCAG TCAAACCTGA TGTCGTCGTA 
CAAGTGACTG TTCTGGCAGA AAACAACCAC ATTAAGGAGC TTCTGAGGAA ACATGGGGTC 
AACGTCCAGA GTATCGCTGA CATTCATCCA ATCCGAGTCC AGCCAGGCCG CATCCTTAGT 
CACATATATG CCAAGCTTGG ACGGAATAAG AATATGAGAT TGAGTGGGCG ACCGTATCGG 
CACATTGGTG TCCTTGGGAC ATCGAAACTA TACGTGATTA GGAACCAGAT CTTTACTTTC 
ACGCCCCAGT TCACCGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATCGTA 
GAGATGCTGA GGATTGAGCT GGCCTACCTG TGCACCTGCT GGCGGATGAC GGGCAGACCC 
ACGCTCACCT TCCCCATCAC GCATACCATG CTCACAAATG ATGGCTCAGA TATTCATTCC 
GCTGTTCTTG CTACAATTCG CAAACTAGAG GATGGATATT TTGGAGGAGC TAGAGTAAAA 
TTAGGGAGTC TTTCGGAATT TCTCACCACC TCTTTCTACA CGTATCTGAC CTTCCTGGAT 
CCAGACTGTG ATGAGAAGTT GTTTGATGAT GCCAGTGAAG GGACCTTCAG TCCTGATAGT 
GATTCAGACC TGGGAGGATA TTTGGAAGAC ACCTATAATC AAGAAAGCCA GGACGAGCTT 
GACCAGTATA TCAACCACCT TCTGCAAAGC ACATCCTCGA AGTGCTATCT GCCACCTCTC 
TGTAAGAAGA CAGAGGACAA CCAGGTTTTC AGTGCTATCC ATTCCACTCG GGATATACTT 
TCTGTGATGG CAAAAGCAAA GGGCTTGGAA ATTCCATTTG TTCCCATGGC TTTGCCAACT 
AAAGTTCTAA GTGCCCACCG TAAATCGCTG AATCTCGTTG ATTCTCCTCA GCCACTCCTA 
AAAAAGGCCC CTGAAGATGA CTTCCAGTTT CCCAGAGATG ACCATGGCGA CGTGGACTGT 
GAGAAGCTGG TTGAGCAGTT GAAAGACTGT TCGAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTGT ATGTAATAAA GGGTCCCAGC TGGGACACAA ATCTCTCTGG ACAGCACGGG 
GTCACTGTTC ACAACCTCCT CAGTGAGCTC TATGCGAAGG CCGGCTTGAA CCAGGAGTGG 
GGCCTGATCC GCTACATCTC CGGCCTCCTC CGGAAGAAAG TGGAGGTCCT GGCTGAGGCG 
TGCACGGACC TGCTGTCACA CCAGAAGCAG CTCACAGTGG GCCTGCCCCC AGAACCCCGG 
GAGAAGACCA TCTCTGCGCC TCTTCCCCCC GAGGAGCTCA CGAAGCTCAT CTACGAAGCC 
AGCGGGCAGG ACATCAGCAT CGCCGTGCTC ACACAGGAGA TCGTGGTGTA CCTGGCGATG 
TACGTCAGGG CACAGCCCAG CCTCTTCGTG GAGATGCTCC GGCTCCGAAT TGGACTGATC 
ATTCAGGTGA TGGCCACGGA GCTGGCACGG AGCCTGAACT GTTCAGGAGA AGAAGCTTCT 
GAAAGTCTGA TGAACCTCAG CCCTTTTGAT ATGAAAAATC TCCTGCACCA CATCTTAAGT 
GGCAAAGAAT TTGGTGTTGA AAGAAGCATG CGGCCCATTC ACTCCTCCAC ACCTAGCCCT 
GCTGTCTCCA TCCATGAGGT GGGCCACACT GGAGTCACCA AAACCGAGAG GAGCGGCATC 
AACAGGCTGA GGAGTGAGAT GAAACAGATG ACTAGGCGGT TTAGTGCTGA TGAACAGAGG 
TCCAGCACCC CATCCTCACC CACGGGCACA TCGTCATCAG ACTCGGGCAG GCAGCACATG 
GGCTGGGGGG AACGGCAGGG CCAGTGGCTG CGCAGGAGAA GGCTGGACGG CGCCATCAAC 
AGGGTCCCCG TGGGATTCTA CCAGAAGGTG TGGAAGATCC TTCAGAAGTG CCATGGTCTG 
TCCATCGACG GTTACGTGCT CCCGTCCTCG ACCACGCAAG AGATGACCCC GCAGGAGATC 
AAGTTTGCTG TCCACGTTGA GTCAGTGCTG AACCGAGTGC CACAACCCGA GTACCGGCAG 
CTGCTGGTGG AGGCCATCAT GGTGCTGACG CTGCTCTCGG ACACAGAGAT GAACAGCATC 
GGGGGCATCA TCCATGTGGA CCAGATCGTG CACATGGCCA ACCAGCTGTT CCTACAAGAC 
CAGGTGTCAA GTGGAGCCAC GGACACGCTG GAGAAAGACC AAGCCACAGG CATCTGCCAC 
TTCTTTTACG ACAGCGCTCC AAGCGGGGCT TATGGGACGA TGACCTACCT AACGAAGGCG 
GTGGCTTCTC ATTTGCAGGA ACTGCTGCCC AATTCAGGTT GCCAGATGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA2 ( XM_024987968.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024843736.1 Bos taurus phosphorylase kinase regulatory subunit alpha 2 (PHKA2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	OBa238362
Clone ID Related Accession (Same CDS sequence)	XM_024987968.1
Accession Version	XM_024987968.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3624bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCA GGAGCAATTC CGGGGTCCGC TTAGACGGAT ACGCGCGGCT GGTGCAGCAA 
ACAATCCTCT GTTACCAGAA TCCCATCACG GGGCTGATGT CAGCCAGTCA TGAGCAGAAG 
GACGCCTGGG TACGGGATAA CATCTACAGC ATCCTGGCCG TGTGGGGCCT GGGCATGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAAT 
GTGGTGAAAC TGATGCGAGG TCTTCTCCAG TGCATGATGA GACAGGTGGA TAAAGTGGAG 
AGGTTCAAGC ACACTCAGAG TACCGAGGAC AGCCTGCACG CCAAGTACAA CACTGCCACC 
TGCAGCACTG TGGTGGGCGA CGACGAGTGG GGCCACCTCC AGGTGGATGC CACCTCCCTC 
TTCCTCCTGT TTCTGGCCCA GATGACAGCC TCCGGCTTAC GTATTATTTT CACTCTTGAC 
GAGGTGGCCT TCATACAGAA TCTTGTTTTT TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGAATGT GGGAACGTGG AGATAAGACT AATCAGGGTA TTCCAGAATT GAATGCAAGC 
TCTGTAGGAA TGGCCAAGGC AGCCCTTGAA GCAATTGATG AACTGGACCT TTTTGGAGCC 
CATGGAGGTC GCAAGTCTGT GATCCACGTC CTGCCGGATG AGGTGGAACA CTGCCAGTCT 
ATTCTGTTCT CCATGCTGCC AAGAGCCTCG ACGTCAAAAG AGATTGATGC TGGGCTTCTT 
TCCATTATTT CTTTCCCGGC CTTCGCGGTG GAAGATGTGA ACCTTGTGAA TGTGACAAAA 
AATGAAATTA TTTCCAAGCT CCAGGGGCGT TATGGATGCT GTCGCTTCCT TCGAGATGGT 
TATAAAACCC CAAGAGAGGA CCCAAACCGA TTGCATTATG ACCCTGCTGA ACTCAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTCATAAT AGATGGAGTC 
TTCAATGGTG ATGCCGTTCA GGTCCAAGAA TACCGTGAAG CCCTGGAGGG GATACTGATC 
AGAGGCAATA ACGGGGTCCA CTTGTTGCCT GAACTCTACG CCATCCCACC TGACAAGGTG 
GATGAAGAGT ATAAGAATCC TCACACGGTG GATAGAGTTC CCCTGGGGAA GGTGCCGCAT 
CTGTGGGGTC AGTCCTTGTA CATCCTCAGC TCCCTGTTGG CAGAGGGATT CCTTGCTGCT 
GGTGAAATTG ATCCCTTGAA CAGAAGATTT TCCACTTCAG TCAAACCTGA TGTCGTCGTA 
CAAGTGACTG TTCTGGCAGA AAACAACCAC ATTAAGGAGC TTCTGAGGAA ACATGGGGTC 
AACGTCCAGA GTATCGCTGA CATTCATCCA ATCCGAGTCC AGCCAGGCCG CATCCTTAGT 
CACATATATG CCAAGCTTGG ACGGAATAAG AATATGAGAT TGAGTGGGCG ACCGTATCGG 
CACATTGGTG TCCTTGGGAC ATCGAAACTA TACGTGATTA GGAACCAGAT CTTTACTTTC 
ACGCCCCAGT TCACCGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATCGTA 
GAGATGCTGA GGATTGAGCT GGCCTACCTG TGCACCTGCT GGCGGATGAC GGGCAGACCC 
ACGCTCACCT TCCCCATCAC GCATACCATG CTCACAAATG ATGGCTCAGA TATTCATTCC 
GCTGTTCTTG CTACAATTCG CAAACTAGAG GATGGATATT TTGGAGGAGC TAGAGTAAAA 
TTAGGGAGTC TTTCGGAATT TCTCACCACC TCTTTCTACA CGTATCTGAC CTTCCTGGAT 
CCAGACTGTG ATGAGAAGTT GTTTGATGAT GCCAGTGAAG GGACCTTCAG TCCTGATAGT 
GATTCAGACC TGGGAGGATA TTTGGAAGAC ACCTATAATC AAGAAAGCCA GGACGAGCTT 
GACCAGTATA TCAACCACCT TCTGCAAAGC ACATCCTCGA AGTGCTATCT GCCACCTCTC 
TGTAAGAAGA CAGAGGACAA CCAGGTTTTC AGTGCTATCC ATTCCACTCG GGATATACTT 
TCTGTGATGG CAAAAGCAAA GGGCTTGGAA ATTCCATTTG TTCCCATGGC TTTGCCAACT 
AAAGTTCTAA GTGCCCACCG TAAATCGCTG AATCTCGTTG ATTCTCCTCA GCCACTCCTA 
AAAAAGGCCC CTGAAGATGA CTTCCAGTTT CCCAGAGATG ACCATGGCGA CGTGGACTGT 
GAGAAGCTGG TTGAGCAGTT GAAAGACTGT TCGAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTGT ATGTAATAAA GGGTCCCAGC TGGGACACAA ATCTCTCTGG ACAGCACGGG 
GTCACTGTTC ACAACCTCCT CAGTGAGCTC TATGCGAAGG CCGGCTTGAA CCAGGAGTGG 
GGCCTGATCC GCTACATCTC CGGCCTCCTC CGGAAGAAAG TGGAGGTCCT GGCTGAGGCG 
TGCACGGACC TGCTGTCACA CCAGAAGCAG CTCACAGTGG GCCTGCCCCC AGAACCCCGG 
GAGAAGACCA TCTCTGCGCC TCTTCCCCCC GAGGAGCTCA CGAAGCTCAT CTACGAAGCC 
AGCGGGCAGG ACATCAGCAT CGCCGTGCTC ACACAGGAGA TCGTGGTGTA CCTGGCGATG 
TACGTCAGGG CACAGCCCAG CCTCTTCGTG GAGATGCTCC GGCTCCGAAT TGGACTGATC 
ATTCAGGTGA TGGCCACGGA GCTGGCACGG AGCCTGAACT GTTCAGGAGA AGAAGCTTCT 
GAAAGTCTGA TGAACCTCAG CCCTTTTGAT ATGAAAAATC TCCTGCACCA CATCTTAAGT 
GGCAAAGAAT TTGGTGTTGA AAGAAGCATG CGGCCCATTC ACTCCTCCAC ACCTAGCCCT 
GCTGTCTCCA TCCATGAGGT GGGCCACACT GGAGTCACCA AAACCGAGAG GAGCGGCATC 
AACAGGCTGA GGAGTGAGAT GAAACAGAGG TCCAGCACCC CATCCTCACC CACGGGCACA 
TCGTCATCAG ACTCGGGCAG GCAGCACATG GGCTGGGGGG AACGGCAGGG CCAGTGGCTG 
CGCAGGAGAA GGCTGGACGG CGCCATCAAC AGGGTCCCCG TGGGATTCTA CCAGAAGGTG 
TGGAAGATCC TTCAGAAGTG CCATGGTCTG TCCATCGACG GTTACGTGCT CCCGTCCTCG 
ACCACGCAAG AGATGACCCC GCAGGAGATC AAGTTTGCTG TCCACGTTGA GTCAGTGCTG 
AACCGAGTGC CACAACCCGA GTACCGGCAG CTGCTGGTGG AGGCCATCAT GGTGCTGACG 
CTGCTCTCGG ACACAGAGAT GAACAGCATC GGGGGCATCA TCCATGTGGA CCAGATCGTG 
CACATGGCCA ACCAGCTGTT CCTACAAGAC CAGGTGTCAA GTGGAGCCAC GGACACGCTG 
GAGAAAGACC AAGCCACAGG CATCTGCCAC TTCTTTTACG ACAGCGCTCC AAGCGGGGCT 
TATGGGACGA TGACCTACCT AACGAAGGCG GTGGCTTCTC ATTTGCAGGA ACTGCTGCCC 
AATTCAGGTT GCCAGATGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024843736.1
CDS	253..3876
Translation	MRSRSNSGVRLDGYARLVQQTILCYQNPITGLMSASHEQKDAWVRDNIYSILAVWGLGMAYRKNADRDEDKAKAYELEQNVVKLMRGLLQCMMRQVDKVERFKHTQSTEDSLHAKYNTATCSTVVGDDEWGHLQVDATSLFLLFLAQMTASGLRIIFTLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGMAKAALEAIDELDLFGAHGGRKSVIHVLPDEVEHCQSILFSMLPRASTSKEIDAGLLSIISFPAFAVEDVNLVNVTKNEIISKLQGRYGCCRFLRDGYKTPREDPNRLHYDPAELKLFENIECEWPVFWTYFIIDGVFNGDAVQVQEYREALEGILIRGNNGVHLLPELYAIPPDKVDEEYKNPHTVDRVPLGKVPHLWGQSLYILSSLLAEGFLAAGEIDPLNRRFSTSVKPDVVVQVTVLAENNHIKELLRKHGVNVQSIADIHPIRVQPGRILSHIYAKLGRNKNMRLSGRPYRHIGVLGTSKLYVIRNQIFTFTPQFTDQHHFYLALDNEMIVEMLRIELAYLCTCWRMTGRPTLTFPITHTMLTNDGSDIHSAVLATIRKLEDGYFGGARVKLGSLSEFLTTSFYTYLTFLDPDCDEKLFDDASEGTFSPDSDSDLGGYLEDTYNQESQDELDQYINHLLQSTSSKCYLPPLCKKTEDNQVFSAIHSTRDILSVMAKAKGLEIPFVPMALPTKVLSAHRKSLNLVDSPQPLLKKAPEDDFQFPRDDHGDVDCEKLVEQLKDCSNLQDQADILYILYVIKGPSWDTNLSGQHGVTVHNLLSELYAKAGLNQEWGLIRYISGLLRKKVEVLAEACTDLLSHQKQLTVGLPPEPREKTISAPLPPEELTKLIYEASGQDISIAVLTQEIVVYLAMYVRAQPSLFVEMLRLRIGLIIQVMATELARSLNCSGEEASESLMNLSPFDMKNLLHHILSGKEFGVERSMRPIHSSTPSPAVSIHEVGHTGVTKTERSGINRLRSEMKQRSSTPSSPTGTSSSDSGRQHMGWGERQGQWLRRRRLDGAINRVPVGFYQKVWKILQKCHGLSIDGYVLPSSTTQEMTPQEIKFAVHVESVLNRVPQPEYRQLLVEAIMVLTLLSDTEMNSIGGIIHVDQIVHMANQLFLQDQVSSGATDTLEKDQATGICHFFYDSAPSGAYGTMTYLTKAVASHLQELLPNSGCQMQ

Target ORF information:

RefSeq Version	XM_024987968.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphorylase kinase regulatory subunit alpha 2 (PHKA2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024987968.1

ORF Insert Sequence:

ATGAGGAGCA GGAGCAATTC CGGGGTCCGC TTAGACGGAT ACGCGCGGCT GGTGCAGCAA 
ACAATCCTCT GTTACCAGAA TCCCATCACG GGGCTGATGT CAGCCAGTCA TGAGCAGAAG 
GACGCCTGGG TACGGGATAA CATCTACAGC ATCCTGGCCG TGTGGGGCCT GGGCATGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAAT 
GTGGTGAAAC TGATGCGAGG TCTTCTCCAG TGCATGATGA GACAGGTGGA TAAAGTGGAG 
AGGTTCAAGC ACACTCAGAG TACCGAGGAC AGCCTGCACG CCAAGTACAA CACTGCCACC 
TGCAGCACTG TGGTGGGCGA CGACGAGTGG GGCCACCTCC AGGTGGATGC CACCTCCCTC 
TTCCTCCTGT TTCTGGCCCA GATGACAGCC TCCGGCTTAC GTATTATTTT CACTCTTGAC 
GAGGTGGCCT TCATACAGAA TCTTGTTTTT TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGAATGT GGGAACGTGG AGATAAGACT AATCAGGGTA TTCCAGAATT GAATGCAAGC 
TCTGTAGGAA TGGCCAAGGC AGCCCTTGAA GCAATTGATG AACTGGACCT TTTTGGAGCC 
CATGGAGGTC GCAAGTCTGT GATCCACGTC CTGCCGGATG AGGTGGAACA CTGCCAGTCT 
ATTCTGTTCT CCATGCTGCC AAGAGCCTCG ACGTCAAAAG AGATTGATGC TGGGCTTCTT 
TCCATTATTT CTTTCCCGGC CTTCGCGGTG GAAGATGTGA ACCTTGTGAA TGTGACAAAA 
AATGAAATTA TTTCCAAGCT CCAGGGGCGT TATGGATGCT GTCGCTTCCT TCGAGATGGT 
TATAAAACCC CAAGAGAGGA CCCAAACCGA TTGCATTATG ACCCTGCTGA ACTCAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTCATAAT AGATGGAGTC 
TTCAATGGTG ATGCCGTTCA GGTCCAAGAA TACCGTGAAG CCCTGGAGGG GATACTGATC 
AGAGGCAATA ACGGGGTCCA CTTGTTGCCT GAACTCTACG CCATCCCACC TGACAAGGTG 
GATGAAGAGT ATAAGAATCC TCACACGGTG GATAGAGTTC CCCTGGGGAA GGTGCCGCAT 
CTGTGGGGTC AGTCCTTGTA CATCCTCAGC TCCCTGTTGG CAGAGGGATT CCTTGCTGCT 
GGTGAAATTG ATCCCTTGAA CAGAAGATTT TCCACTTCAG TCAAACCTGA TGTCGTCGTA 
CAAGTGACTG TTCTGGCAGA AAACAACCAC ATTAAGGAGC TTCTGAGGAA ACATGGGGTC 
AACGTCCAGA GTATCGCTGA CATTCATCCA ATCCGAGTCC AGCCAGGCCG CATCCTTAGT 
CACATATATG CCAAGCTTGG ACGGAATAAG AATATGAGAT TGAGTGGGCG ACCGTATCGG 
CACATTGGTG TCCTTGGGAC ATCGAAACTA TACGTGATTA GGAACCAGAT CTTTACTTTC 
ACGCCCCAGT TCACCGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATCGTA 
GAGATGCTGA GGATTGAGCT GGCCTACCTG TGCACCTGCT GGCGGATGAC GGGCAGACCC 
ACGCTCACCT TCCCCATCAC GCATACCATG CTCACAAATG ATGGCTCAGA TATTCATTCC 
GCTGTTCTTG CTACAATTCG CAAACTAGAG GATGGATATT TTGGAGGAGC TAGAGTAAAA 
TTAGGGAGTC TTTCGGAATT TCTCACCACC TCTTTCTACA CGTATCTGAC CTTCCTGGAT 
CCAGACTGTG ATGAGAAGTT GTTTGATGAT GCCAGTGAAG GGACCTTCAG TCCTGATAGT 
GATTCAGACC TGGGAGGATA TTTGGAAGAC ACCTATAATC AAGAAAGCCA GGACGAGCTT 
GACCAGTATA TCAACCACCT TCTGCAAAGC ACATCCTCGA AGTGCTATCT GCCACCTCTC 
TGTAAGAAGA CAGAGGACAA CCAGGTTTTC AGTGCTATCC ATTCCACTCG GGATATACTT 
TCTGTGATGG CAAAAGCAAA GGGCTTGGAA ATTCCATTTG TTCCCATGGC TTTGCCAACT 
AAAGTTCTAA GTGCCCACCG TAAATCGCTG AATCTCGTTG ATTCTCCTCA GCCACTCCTA 
AAAAAGGCCC CTGAAGATGA CTTCCAGTTT CCCAGAGATG ACCATGGCGA CGTGGACTGT 
GAGAAGCTGG TTGAGCAGTT GAAAGACTGT TCGAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTGT ATGTAATAAA GGGTCCCAGC TGGGACACAA ATCTCTCTGG ACAGCACGGG 
GTCACTGTTC ACAACCTCCT CAGTGAGCTC TATGCGAAGG CCGGCTTGAA CCAGGAGTGG 
GGCCTGATCC GCTACATCTC CGGCCTCCTC CGGAAGAAAG TGGAGGTCCT GGCTGAGGCG 
TGCACGGACC TGCTGTCACA CCAGAAGCAG CTCACAGTGG GCCTGCCCCC AGAACCCCGG 
GAGAAGACCA TCTCTGCGCC TCTTCCCCCC GAGGAGCTCA CGAAGCTCAT CTACGAAGCC 
AGCGGGCAGG ACATCAGCAT CGCCGTGCTC ACACAGGAGA TCGTGGTGTA CCTGGCGATG 
TACGTCAGGG CACAGCCCAG CCTCTTCGTG GAGATGCTCC GGCTCCGAAT TGGACTGATC 
ATTCAGGTGA TGGCCACGGA GCTGGCACGG AGCCTGAACT GTTCAGGAGA AGAAGCTTCT 
GAAAGTCTGA TGAACCTCAG CCCTTTTGAT ATGAAAAATC TCCTGCACCA CATCTTAAGT 
GGCAAAGAAT TTGGTGTTGA AAGAAGCATG CGGCCCATTC ACTCCTCCAC ACCTAGCCCT 
GCTGTCTCCA TCCATGAGGT GGGCCACACT GGAGTCACCA AAACCGAGAG GAGCGGCATC 
AACAGGCTGA GGAGTGAGAT GAAACAGAGG TCCAGCACCC CATCCTCACC CACGGGCACA 
TCGTCATCAG ACTCGGGCAG GCAGCACATG GGCTGGGGGG AACGGCAGGG CCAGTGGCTG 
CGCAGGAGAA GGCTGGACGG CGCCATCAAC AGGGTCCCCG TGGGATTCTA CCAGAAGGTG 
TGGAAGATCC TTCAGAAGTG CCATGGTCTG TCCATCGACG GTTACGTGCT CCCGTCCTCG 
ACCACGCAAG AGATGACCCC GCAGGAGATC AAGTTTGCTG TCCACGTTGA GTCAGTGCTG 
AACCGAGTGC CACAACCCGA GTACCGGCAG CTGCTGGTGG AGGCCATCAT GGTGCTGACG 
CTGCTCTCGG ACACAGAGAT GAACAGCATC GGGGGCATCA TCCATGTGGA CCAGATCGTG 
CACATGGCCA ACCAGCTGTT CCTACAAGAC CAGGTGTCAA GTGGAGCCAC GGACACGCTG 
GAGAAAGACC AAGCCACAGG CATCTGCCAC TTCTTTTACG ACAGCGCTCC AAGCGGGGCT 
TATGGGACGA TGACCTACCT AACGAAGGCG GTGGCTTCTC ATTTGCAGGA ACTGCTGCCC 
AATTCAGGTT GCCAGATGCA GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA2 ( XM_024987969.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024843737.1 Bos taurus phosphorylase kinase regulatory subunit alpha 2 (PHKA2), transcript variant X4, mRNA.

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CloneID	OBa238363
Clone ID Related Accession (Same CDS sequence)	XM_024987969.1
Accession Version	XM_024987969.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3612bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGCCA GTCATGAGCA GAAGGACGCC TGGGTACGGG ATAACATCTA CAGCATCCTG 
GCCGTGTGGG GCCTGGGCAT GGCCTACCGC AAGAACGCCG ACCGCGACGA GGACAAGGCC 
AAGGCCTACG AGCTGGAGCA GAATGTGGTG AAACTGATGC GAGGTCTTCT CCAGTGCATG 
ATGAGACAGG TGGATAAAGT GGAGAGGTTC AAGCACACTC AGAGTACCGA GGACAGCCTG 
CACGCCAAGT ACAACACTGC CACCTGCAGC ACTGTGGTGG GCGACGACGA GTGGGGCCAC 
CTCCAGGTGG ATGCCACCTC CCTCTTCCTC CTGTTTCTGG CCCAGATGAC AGCCTCCGGC 
TTACGTATTA TTTTCACTCT TGACGAGGTG GCCTTCATAC AGAATCTTGT TTTTTACATA 
GAAGCTGCAT ATAAAGTTGC TGATTATGGA ATGTGGGAAC GTGGAGATAA GACTAATCAG 
GGTATTCCAG AATTGAATGC AAGCTCTGTA GGAATGGCCA AGGCAGCCCT TGAAGCAATT 
GATGAACTGG ACCTTTTTGG AGCCCATGGA GGTCGCAAGT CTGTGATCCA CGTCCTGCCG 
GATGAGGTGG AACACTGCCA GTCTATTCTG TTCTCCATGC TGCCAAGAGC CTCGACGTCA 
AAAGAGATTG ATGCTGGGCT TCTTTCCATT ATTTCTTTCC CGGCCTTCGC GGTGGAAGAT 
GTGAACCTTG TGAATGTGAC AAAAAATGAA ATTATTTCCA AGCTCCAGGG GCGTTATGGA 
TGCTGTCGCT TCCTTCGAGA TGGTTATAAA ACCCCAAGAG AGGACCCAAA CCGATTGCAT 
TATGACCCTG CTGAACTCAA GCTCTTTGAA AACATTGAAT GTGAATGGCC TGTGTTCTGG 
ACTTATTTCA TAATAGATGG AGTCTTCAAT GGTGATGCCG TTCAGGTCCA AGAATACCGT 
GAAGCCCTGG AGGGGATACT GATCAGAGGC AATAACGGGG TCCACTTGTT GCCTGAACTC 
TACGCCATCC CACCTGACAA GGTGGATGAA GAGTATAAGA ATCCTCACAC GGTGGATAGA 
GTTCCCCTGG GGAAGGTGCC GCATCTGTGG GGTCAGTCCT TGTACATCCT CAGCTCCCTG 
TTGGCAGAGG GATTCCTTGC TGCTGGTGAA ATTGATCCCT TGAACAGAAG ATTTTCCACT 
TCAGTCAAAC CTGATGTCGT CGTACAAGTG ACTGTTCTGG CAGAAAACAA CCACATTAAG 
GAGCTTCTGA GGAAACATGG GGTCAACGTC CAGAGTATCG CTGACATTCA TCCAATCCGA 
GTCCAGCCAG GCCGCATCCT TAGTCACATA TATGCCAAGC TTGGACGGAA TAAGAATATG 
AGATTGAGTG GGCGACCGTA TCGGCACATT GGTGTCCTTG GGACATCGAA ACTATACGTG 
ATTAGGAACC AGATCTTTAC TTTCACGCCC CAGTTCACCG ACCAGCATCA CTTCTACCTG 
GCCCTGGACA ATGAGATGAT CGTAGAGATG CTGAGGATTG AGCTGGCCTA CCTGTGCACC 
TGCTGGCGGA TGACGGGCAG ACCCACGCTC ACCTTCCCCA TCACGCATAC CATGCTCACA 
AATGATGGCT CAGATATTCA TTCCGCTGTT CTTGCTACAA TTCGCAAACT AGAGGATGGA 
TATTTTGGAG GAGCTAGAGT AAAATTAGGG AGTCTTTCGG AATTTCTCAC CACCTCTTTC 
TACACGTATC TGACCTTCCT GGATCCAGAC TGTGATGAGA AGTTGTTTGA TGATGCCAGT 
GAAGGGACCT TCAGTCCTGA TAGTGATTCA GACCTGGGAG GATATTTGGA AGACACCTAT 
AATCAAGAAA GCCAGGACGA GCTTGACCAG TATATCAACC ACCTTCTGCA AAGCACATCC 
TCGAAGTGCT ATCTGCCACC TCTCTGTAAG AAGACAGAGG ACAACCAGGT TTTCAGTGCT 
ATCCATTCCA CTCGGGATAT ACTTTCTGTG ATGGCAAAAG CAAAGGGCTT GGAAATTCCA 
TTTGTTCCCA TGGCTTTGCC AACTAAAGTT CTAAGTGCCC ACCGTAAATC GCTGAATCTC 
GTTGATTCTC CTCAGCCACT CCTAAAAAAG GCCCCTGAAG ATGACTTCCA GTTTCCCAGA 
GATGACCATG GCGACGTGGA CTGTGAGAAG CTGGTTGAGC AGTTGAAAGA CTGTTCGAAC 
CTACAGGACC AAGCAGACAT TCTGTACATT CTGTATGTAA TAAAGGGTCC CAGCTGGGAC 
ACAAATCTCT CTGGACAGCA CGGGGTCACT GTTCACAACC TCCTCAGTGA GCTCTATGCG 
AAGGCCGGCT TGAACCAGGA GTGGGGCCTG ATCCGCTACA TCTCCGGCCT CCTCCGGAAG 
AAAGTGGAGG TCCTGGCTGA GGCGTGCACG GACCTGCTGT CACACCAGAA GCAGCTCACA 
GTGGGCCTGC CCCCAGAACC CCGGGAGAAG ACCATCTCTG CGCCTCTTCC CCCCGAGGAG 
CTCACGAAGC TCATCTACGA AGCCAGCGGG CAGGACATCA GCATCGCCGT GCTCACACAG 
GAGATCGTGG TGTACCTGGC GATGTACGTC AGGGCACAGC CCAGCCTCTT CGTGGAGATG 
CTCCGGCTCC GAATTGGACT GATCATTCAG GTGATGGCCA CGGAGCTGGC ACGGAGCCTG 
AACTGTTCAG GAGAAGAAGC TTCTGAAAGT CTGATGAACC TCAGCCCTTT TGATATGAAA 
AATCTCCTGC ACCACATCTT AAGTGGCAAA GAATTTGGTG TTGAAAGAAG CATGCGGCCC 
ATTCACTCCT CCACACCTAG CCCTGCTGTC TCCATCCATG AGGTGGGCCA CACTGGAGTC 
ACCAAAACCG AGAGGAGCGG CATCAACAGG CTGAGGAGTG AGATGAAACA GATGACTAGG 
CGGTTTAGTG CTGATGAACA GCTCATTTCT GTGAGCCAGG CCGCATCCAG CAGCGCGCGC 
TCCTCAAAGT CTGCGAGGTC CAGCACCCCA TCCTCACCCA CGGGCACATC GTCATCAGAC 
TCGGGCAGGC AGCACATGGG CTGGGGGGAA CGGCAGGGCC AGTGGCTGCG CAGGAGAAGG 
CTGGACGGCG CCATCAACAG GGTCCCCGTG GGATTCTACC AGAAGGTGTG GAAGATCCTT 
CAGAAGTGCC ATGGTCTGTC CATCGACGGT TACGTGCTCC CGTCCTCGAC CACGCAAGAG 
ATGACCCCGC AGGAGATCAA GTTTGCTGTC CACGTTGAGT CAGTGCTGAA CCGAGTGCCA 
CAACCCGAGT ACCGGCAGCT GCTGGTGGAG GCCATCATGG TGCTGACGCT GCTCTCGGAC 
ACAGAGATGA ACAGCATCGG GGGCATCATC CATGTGGACC AGATCGTGCA CATGGCCAAC 
CAGCTGTTCC TACAAGACCA GGTGTCAAGT GGAGCCACGG ACACGCTGGA GAAAGACCAA 
GCCACAGGCA TCTGCCACTT CTTTTACGAC AGCGCTCCAA GCGGGGCTTA TGGGACGATG 
ACCTACCTAA CGAAGGCGGT GGCTTCTCAT TTGCAGGAAC TGCTGCCCAA TTCAGGTTGC 
CAGATGCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024843737.1
CDS	149..3760
Translation	MSASHEQKDAWVRDNIYSILAVWGLGMAYRKNADRDEDKAKAYELEQNVVKLMRGLLQCMMRQVDKVERFKHTQSTEDSLHAKYNTATCSTVVGDDEWGHLQVDATSLFLLFLAQMTASGLRIIFTLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGMAKAALEAIDELDLFGAHGGRKSVIHVLPDEVEHCQSILFSMLPRASTSKEIDAGLLSIISFPAFAVEDVNLVNVTKNEIISKLQGRYGCCRFLRDGYKTPREDPNRLHYDPAELKLFENIECEWPVFWTYFIIDGVFNGDAVQVQEYREALEGILIRGNNGVHLLPELYAIPPDKVDEEYKNPHTVDRVPLGKVPHLWGQSLYILSSLLAEGFLAAGEIDPLNRRFSTSVKPDVVVQVTVLAENNHIKELLRKHGVNVQSIADIHPIRVQPGRILSHIYAKLGRNKNMRLSGRPYRHIGVLGTSKLYVIRNQIFTFTPQFTDQHHFYLALDNEMIVEMLRIELAYLCTCWRMTGRPTLTFPITHTMLTNDGSDIHSAVLATIRKLEDGYFGGARVKLGSLSEFLTTSFYTYLTFLDPDCDEKLFDDASEGTFSPDSDSDLGGYLEDTYNQESQDELDQYINHLLQSTSSKCYLPPLCKKTEDNQVFSAIHSTRDILSVMAKAKGLEIPFVPMALPTKVLSAHRKSLNLVDSPQPLLKKAPEDDFQFPRDDHGDVDCEKLVEQLKDCSNLQDQADILYILYVIKGPSWDTNLSGQHGVTVHNLLSELYAKAGLNQEWGLIRYISGLLRKKVEVLAEACTDLLSHQKQLTVGLPPEPREKTISAPLPPEELTKLIYEASGQDISIAVLTQEIVVYLAMYVRAQPSLFVEMLRLRIGLIIQVMATELARSLNCSGEEASESLMNLSPFDMKNLLHHILSGKEFGVERSMRPIHSSTPSPAVSIHEVGHTGVTKTERSGINRLRSEMKQMTRRFSADEQLISVSQAASSSARSSKSARSSTPSSPTGTSSSDSGRQHMGWGERQGQWLRRRRLDGAINRVPVGFYQKVWKILQKCHGLSIDGYVLPSSTTQEMTPQEIKFAVHVESVLNRVPQPEYRQLLVEAIMVLTLLSDTEMNSIGGIIHVDQIVHMANQLFLQDQVSSGATDTLEKDQATGICHFFYDSAPSGAYGTMTYLTKAVASHLQELLPNSGCQMQ

Target ORF information:

RefSeq Version	XM_024987969.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphorylase kinase regulatory subunit alpha 2 (PHKA2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024987969.1

ORF Insert Sequence:

ATGTCAGCCA GTCATGAGCA GAAGGACGCC TGGGTACGGG ATAACATCTA CAGCATCCTG 
GCCGTGTGGG GCCTGGGCAT GGCCTACCGC AAGAACGCCG ACCGCGACGA GGACAAGGCC 
AAGGCCTACG AGCTGGAGCA GAATGTGGTG AAACTGATGC GAGGTCTTCT CCAGTGCATG 
ATGAGACAGG TGGATAAAGT GGAGAGGTTC AAGCACACTC AGAGTACCGA GGACAGCCTG 
CACGCCAAGT ACAACACTGC CACCTGCAGC ACTGTGGTGG GCGACGACGA GTGGGGCCAC 
CTCCAGGTGG ATGCCACCTC CCTCTTCCTC CTGTTTCTGG CCCAGATGAC AGCCTCCGGC 
TTACGTATTA TTTTCACTCT TGACGAGGTG GCCTTCATAC AGAATCTTGT TTTTTACATA 
GAAGCTGCAT ATAAAGTTGC TGATTATGGA ATGTGGGAAC GTGGAGATAA GACTAATCAG 
GGTATTCCAG AATTGAATGC AAGCTCTGTA GGAATGGCCA AGGCAGCCCT TGAAGCAATT 
GATGAACTGG ACCTTTTTGG AGCCCATGGA GGTCGCAAGT CTGTGATCCA CGTCCTGCCG 
GATGAGGTGG AACACTGCCA GTCTATTCTG TTCTCCATGC TGCCAAGAGC CTCGACGTCA 
AAAGAGATTG ATGCTGGGCT TCTTTCCATT ATTTCTTTCC CGGCCTTCGC GGTGGAAGAT 
GTGAACCTTG TGAATGTGAC AAAAAATGAA ATTATTTCCA AGCTCCAGGG GCGTTATGGA 
TGCTGTCGCT TCCTTCGAGA TGGTTATAAA ACCCCAAGAG AGGACCCAAA CCGATTGCAT 
TATGACCCTG CTGAACTCAA GCTCTTTGAA AACATTGAAT GTGAATGGCC TGTGTTCTGG 
ACTTATTTCA TAATAGATGG AGTCTTCAAT GGTGATGCCG TTCAGGTCCA AGAATACCGT 
GAAGCCCTGG AGGGGATACT GATCAGAGGC AATAACGGGG TCCACTTGTT GCCTGAACTC 
TACGCCATCC CACCTGACAA GGTGGATGAA GAGTATAAGA ATCCTCACAC GGTGGATAGA 
GTTCCCCTGG GGAAGGTGCC GCATCTGTGG GGTCAGTCCT TGTACATCCT CAGCTCCCTG 
TTGGCAGAGG GATTCCTTGC TGCTGGTGAA ATTGATCCCT TGAACAGAAG ATTTTCCACT 
TCAGTCAAAC CTGATGTCGT CGTACAAGTG ACTGTTCTGG CAGAAAACAA CCACATTAAG 
GAGCTTCTGA GGAAACATGG GGTCAACGTC CAGAGTATCG CTGACATTCA TCCAATCCGA 
GTCCAGCCAG GCCGCATCCT TAGTCACATA TATGCCAAGC TTGGACGGAA TAAGAATATG 
AGATTGAGTG GGCGACCGTA TCGGCACATT GGTGTCCTTG GGACATCGAA ACTATACGTG 
ATTAGGAACC AGATCTTTAC TTTCACGCCC CAGTTCACCG ACCAGCATCA CTTCTACCTG 
GCCCTGGACA ATGAGATGAT CGTAGAGATG CTGAGGATTG AGCTGGCCTA CCTGTGCACC 
TGCTGGCGGA TGACGGGCAG ACCCACGCTC ACCTTCCCCA TCACGCATAC CATGCTCACA 
AATGATGGCT CAGATATTCA TTCCGCTGTT CTTGCTACAA TTCGCAAACT AGAGGATGGA 
TATTTTGGAG GAGCTAGAGT AAAATTAGGG AGTCTTTCGG AATTTCTCAC CACCTCTTTC 
TACACGTATC TGACCTTCCT GGATCCAGAC TGTGATGAGA AGTTGTTTGA TGATGCCAGT 
GAAGGGACCT TCAGTCCTGA TAGTGATTCA GACCTGGGAG GATATTTGGA AGACACCTAT 
AATCAAGAAA GCCAGGACGA GCTTGACCAG TATATCAACC ACCTTCTGCA AAGCACATCC 
TCGAAGTGCT ATCTGCCACC TCTCTGTAAG AAGACAGAGG ACAACCAGGT TTTCAGTGCT 
ATCCATTCCA CTCGGGATAT ACTTTCTGTG ATGGCAAAAG CAAAGGGCTT GGAAATTCCA 
TTTGTTCCCA TGGCTTTGCC AACTAAAGTT CTAAGTGCCC ACCGTAAATC GCTGAATCTC 
GTTGATTCTC CTCAGCCACT CCTAAAAAAG GCCCCTGAAG ATGACTTCCA GTTTCCCAGA 
GATGACCATG GCGACGTGGA CTGTGAGAAG CTGGTTGAGC AGTTGAAAGA CTGTTCGAAC 
CTACAGGACC AAGCAGACAT TCTGTACATT CTGTATGTAA TAAAGGGTCC CAGCTGGGAC 
ACAAATCTCT CTGGACAGCA CGGGGTCACT GTTCACAACC TCCTCAGTGA GCTCTATGCG 
AAGGCCGGCT TGAACCAGGA GTGGGGCCTG ATCCGCTACA TCTCCGGCCT CCTCCGGAAG 
AAAGTGGAGG TCCTGGCTGA GGCGTGCACG GACCTGCTGT CACACCAGAA GCAGCTCACA 
GTGGGCCTGC CCCCAGAACC CCGGGAGAAG ACCATCTCTG CGCCTCTTCC CCCCGAGGAG 
CTCACGAAGC TCATCTACGA AGCCAGCGGG CAGGACATCA GCATCGCCGT GCTCACACAG 
GAGATCGTGG TGTACCTGGC GATGTACGTC AGGGCACAGC CCAGCCTCTT CGTGGAGATG 
CTCCGGCTCC GAATTGGACT GATCATTCAG GTGATGGCCA CGGAGCTGGC ACGGAGCCTG 
AACTGTTCAG GAGAAGAAGC TTCTGAAAGT CTGATGAACC TCAGCCCTTT TGATATGAAA 
AATCTCCTGC ACCACATCTT AAGTGGCAAA GAATTTGGTG TTGAAAGAAG CATGCGGCCC 
ATTCACTCCT CCACACCTAG CCCTGCTGTC TCCATCCATG AGGTGGGCCA CACTGGAGTC 
ACCAAAACCG AGAGGAGCGG CATCAACAGG CTGAGGAGTG AGATGAAACA GATGACTAGG 
CGGTTTAGTG CTGATGAACA GCTCATTTCT GTGAGCCAGG CCGCATCCAG CAGCGCGCGC 
TCCTCAAAGT CTGCGAGGTC CAGCACCCCA TCCTCACCCA CGGGCACATC GTCATCAGAC 
TCGGGCAGGC AGCACATGGG CTGGGGGGAA CGGCAGGGCC AGTGGCTGCG CAGGAGAAGG 
CTGGACGGCG CCATCAACAG GGTCCCCGTG GGATTCTACC AGAAGGTGTG GAAGATCCTT 
CAGAAGTGCC ATGGTCTGTC CATCGACGGT TACGTGCTCC CGTCCTCGAC CACGCAAGAG 
ATGACCCCGC AGGAGATCAA GTTTGCTGTC CACGTTGAGT CAGTGCTGAA CCGAGTGCCA 
CAACCCGAGT ACCGGCAGCT GCTGGTGGAG GCCATCATGG TGCTGACGCT GCTCTCGGAC 
ACAGAGATGA ACAGCATCGG GGGCATCATC CATGTGGACC AGATCGTGCA CATGGCCAAC 
CAGCTGTTCC TACAAGACCA GGTGTCAAGT GGAGCCACGG ACACGCTGGA GAAAGACCAA 
GCCACAGGCA TCTGCCACTT CTTTTACGAC AGCGCTCCAA GCGGGGCTTA TGGGACGATG 
ACCTACCTAA CGAAGGCGGT GGCTTCTCAT TTGCAGGAAC TGCTGCCCAA TTCAGGTTGC 
CAGATGCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA2 ( XM_024987970.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024843738.1 Bos taurus phosphorylase kinase regulatory subunit alpha 2 (PHKA2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa238364
Clone ID Related Accession (Same CDS sequence)	XM_024987970.1
Accession Version	XM_024987970.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3606bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCA GGAGCAATTC CGGGGTCCGC TTAGACGGAT ACGCGCGGCT GGTGCAGCAA 
ACAATCCTCT GTTACCAGAA TCCCATCACG GGGCTGATGT CAGCCAGTCA TGAGCAGAAG 
GACGCCTGGG TACGGGATAA CATCTACAGC ATCCTGGCCG TGTGGGGCCT GGGCATGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAAT 
GTGGTGAAAC TGATGCGAGG TCTTCTCCAG TGCATGATGA GACAGGTGGA TAAAGTGGAG 
AGGTTCAAGC ACACTCAGAG TACCGAGGAC AGCCTGCACG CCAAGTACAA CACTGCCACC 
TGCAGCACTG TGGTGGGCGA CGACGAGTGG GGCCACCTCC AGGTGGATGC CACCTCCCTC 
TTCCTCCTGT TTCTGGCCCA GATGACAGCC TCCGGCTTAC GTATTATTTT CACTCTTGAC 
GAGGTGGCCT TCATACAGAA TCTTGTTTTT TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGAATGT GGGAACGTGG AGATAAGACT AATCAGGGTA TTCCAGAATT GAATGCAAGC 
TCTGTAGGAA TGGCCAAGGC AGCCCTTGAA GCAATTGATG AACTGGACCT TTTTGGAGCC 
CATGGAGGTC GCAAGTCTGT GATCCACGTC CTGCCGGATG AGGTGGAACA CTGCCAGTCT 
ATTCTGTTCT CCATGCTGCC AAGAGCCTCG ACGTCAAAAG AGATTGATGC TGGGCTTCTT 
TCCATTATTT CTTTCCCGGC CTTCGCGGTG GAAGATGTGA ACCTTGTGAA TGTGACAAAA 
AATGAAATTA TTTCCAAGCT CCAGGGGCGT TATGGATGCT GTCGCTTCCT TCGAGATGGT 
TATAAAACCC CAAGAGAGGA CCCAAACCGA TTGCATTATG ACCCTGCTGA ACTCAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTCATAAT AGATGGAGTC 
TTCAATGGTG ATGCCGTTCA GGTCCAAGAA TACCGTGAAG CCCTGGAGGG GATACTGATC 
AGAGGCAATA ACGGGGTCCA CTTGTTGCCT GAACTCTACG CCATCCCACC TGACAAGGTG 
GATGAAGAGT ATAAGAATCC TCACACGGTG GATAGAGTTC CCCTGGGGAA GGTGCCGCAT 
CTGTGGGGTC AGTCCTTGTA CATCCTCAGC TCCCTGTTGG CAGAGGGATT CCTTGCTGCT 
GGTGAAATTG ATCCCTTGAA CAGAAGATTT TCCACTTCAG TCAAACCTGA TGTCGTCGTA 
CAAGTGACTG TTCTGGCAGA AAACAACCAC ATTAAGGAGC TTCTGAGGAA ACATGGGGTC 
AACGTCCAGA GTATCGCTGA CATTCATCCA ATCCGAGTCC AGCCAGGCCG CATCCTTAGT 
CACATATATG CCAAGCTTGG ACGGAATAAG AATATGAGAT TGAGTGGGCG ACCGTATCGG 
CACATTGGTG TCCTTGGGAC ATCGAAACTA TACGTGATTA GGAACCAGAT CTTTACTTTC 
ACGCCCCAGT TCACCGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATCGTA 
GAGATGCTGA GGATTGAGCT GGCCTACCTG TGCACCTGCT GGCGGATGAC GGGCAGACCC 
ACGCTCACCT TCCCCATCAC GCATACCATG CTCACAAATG ATGGCTCAGA TATTCATTCC 
GCTGTTCTTG CTACAATTCG CAAACTAGAG GATGGATATT TTGGAGGAGC TAGAGTAAAA 
TTAGGGAGTC TTTCGGAATT TCTCACCACC TCTTTCTACA CGTATCTGAC CTTCCTGGAT 
CCAGACTGTG ATGAGAAGTT GTTTGATGAT GCCAGTGAAG GGACCTTCAG TCCTGATAGT 
GATTCAGACC TGGGAGGATA TTTGGAAGAC ACCTATAATC AAGAAAGCCA GGACGAGCTT 
GACCAGTATA TCAACCACCT TCTGCAAAGC ACATCCTCGA AGTGCTATCT GCCACCTCTC 
TGTAAGAAGA CAGAGGACAA CCAGGTTTTC AGTGCTATCC ATTCCACTCG GGATATACTT 
TCTGTGATGG CAAAAGCAAA GGGCTTGGAA ATTCCATTTG TTCCCATGGC TTTGCCAACT 
AAAGTTCTAA GTGCCCACCG TAAATCGCTG AATCTCGTTG ATTCTCCTCA GCCACTCCTA 
AAAAAGGCCC CTGAAGATGA CTTCCAGTTT CCCAGAGATG ACCATGGCGA CGTGGACTGT 
GAGAAGCTGG TTGAGCAGTT GAAAGACTGT TCGAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTGT ATGTAATAAA GGGTCCCAGC TGGGACACAA ATCTCTCTGG ACAGCACGGG 
GTCACTGTTC ACAACCTCCT CAGTGAGCTC TATGCGAAGG CCGGCTTGAA CCAGGAGTGG 
GGCCTGATCC GCTACATCTC CGGCCTCCTC CGGAAGAAAG TGGAGGTCCT GGCTGAGGCG 
TGCACGGACC TGCTGTCACA CCAGAAGCAG CTCACAGTGG GCCTGCCCCC AGAACCCCGG 
GAGAAGACCA TCTCTGCGCC TCTTCCCCCC GAGGAGCTCA CGAAGCTCAT CTACGAAGCC 
AGCGGGCAGG ACATCAGCAT CGCCGTGCTC ACACAGGAGA TCGTGGTGTA CCTGGCGATG 
TACGTCAGGG CACAGCCCAG CCTCTTCGTG GAGATGCTCC GGCTCCGAAT TGGACTGATC 
ATTCAGGTGA TGGCCACGGA GCTGGCACGG AGCCTGAACT GTTCAGTGCG GCCCATTCAC 
TCCTCCACAC CTAGCCCTGC TGTCTCCATC CATGAGGTGG GCCACACTGG AGTCACCAAA 
ACCGAGAGGA GCGGCATCAA CAGGCTGAGG AGTGAGATGA AACAGATGAC TAGGCGGTTT 
AGTGCTGATG AACAGCTCAT TTCTGTGAGC CAGGCCGCAT CCAGCAGCGC GCGCTCCTCA 
AAGTCTGCGA GGTCCAGCAC CCCATCCTCA CCCACGGGCA CATCGTCATC AGACTCGGGC 
AGGCAGCACA TGGGCTGGGG GGAACGGCAG GGCCAGTGGC TGCGCAGGAG AAGGCTGGAC 
GGCGCCATCA ACAGGGTCCC CGTGGGATTC TACCAGAAGG TGTGGAAGAT CCTTCAGAAG 
TGCCATGGTC TGTCCATCGA CGGTTACGTG CTCCCGTCCT CGACCACGCA AGAGATGACC 
CCGCAGGAGA TCAAGTTTGC TGTCCACGTT GAGTCAGTGC TGAACCGAGT GCCACAACCC 
GAGTACCGGC AGCTGCTGGT GGAGGCCATC ATGGTGCTGA CGCTGCTCTC GGACACAGAG 
ATGAACAGCA TCGGGGGCAT CATCCATGTG GACCAGATCG TGCACATGGC CAACCAGCTG 
TTCCTACAAG ACCAGGTGTC AAGTGGAGCC ACGGACACGC TGGAGAAAGA CCAAGCCACA 
GGCATCTGCC ACTTCTTTTA CGACAGCGCT CCAAGCGGGG CTTATGGGAC GATGACCTAC 
CTAACGAAGG CGGTGGCTTC TCATTTGCAG GAACTGCTGC CCAATTCAGG TTGCCAGATG 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024843738.1
CDS	253..3858
Translation	MRSRSNSGVRLDGYARLVQQTILCYQNPITGLMSASHEQKDAWVRDNIYSILAVWGLGMAYRKNADRDEDKAKAYELEQNVVKLMRGLLQCMMRQVDKVERFKHTQSTEDSLHAKYNTATCSTVVGDDEWGHLQVDATSLFLLFLAQMTASGLRIIFTLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGMAKAALEAIDELDLFGAHGGRKSVIHVLPDEVEHCQSILFSMLPRASTSKEIDAGLLSIISFPAFAVEDVNLVNVTKNEIISKLQGRYGCCRFLRDGYKTPREDPNRLHYDPAELKLFENIECEWPVFWTYFIIDGVFNGDAVQVQEYREALEGILIRGNNGVHLLPELYAIPPDKVDEEYKNPHTVDRVPLGKVPHLWGQSLYILSSLLAEGFLAAGEIDPLNRRFSTSVKPDVVVQVTVLAENNHIKELLRKHGVNVQSIADIHPIRVQPGRILSHIYAKLGRNKNMRLSGRPYRHIGVLGTSKLYVIRNQIFTFTPQFTDQHHFYLALDNEMIVEMLRIELAYLCTCWRMTGRPTLTFPITHTMLTNDGSDIHSAVLATIRKLEDGYFGGARVKLGSLSEFLTTSFYTYLTFLDPDCDEKLFDDASEGTFSPDSDSDLGGYLEDTYNQESQDELDQYINHLLQSTSSKCYLPPLCKKTEDNQVFSAIHSTRDILSVMAKAKGLEIPFVPMALPTKVLSAHRKSLNLVDSPQPLLKKAPEDDFQFPRDDHGDVDCEKLVEQLKDCSNLQDQADILYILYVIKGPSWDTNLSGQHGVTVHNLLSELYAKAGLNQEWGLIRYISGLLRKKVEVLAEACTDLLSHQKQLTVGLPPEPREKTISAPLPPEELTKLIYEASGQDISIAVLTQEIVVYLAMYVRAQPSLFVEMLRLRIGLIIQVMATELARSLNCSVRPIHSSTPSPAVSIHEVGHTGVTKTERSGINRLRSEMKQMTRRFSADEQLISVSQAASSSARSSKSARSSTPSSPTGTSSSDSGRQHMGWGERQGQWLRRRRLDGAINRVPVGFYQKVWKILQKCHGLSIDGYVLPSSTTQEMTPQEIKFAVHVESVLNRVPQPEYRQLLVEAIMVLTLLSDTEMNSIGGIIHVDQIVHMANQLFLQDQVSSGATDTLEKDQATGICHFFYDSAPSGAYGTMTYLTKAVASHLQELLPNSGCQMQ

Target ORF information:

RefSeq Version	XM_024987970.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphorylase kinase regulatory subunit alpha 2 (PHKA2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024987970.1

ORF Insert Sequence:

ATGAGGAGCA GGAGCAATTC CGGGGTCCGC TTAGACGGAT ACGCGCGGCT GGTGCAGCAA 
ACAATCCTCT GTTACCAGAA TCCCATCACG GGGCTGATGT CAGCCAGTCA TGAGCAGAAG 
GACGCCTGGG TACGGGATAA CATCTACAGC ATCCTGGCCG TGTGGGGCCT GGGCATGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAAT 
GTGGTGAAAC TGATGCGAGG TCTTCTCCAG TGCATGATGA GACAGGTGGA TAAAGTGGAG 
AGGTTCAAGC ACACTCAGAG TACCGAGGAC AGCCTGCACG CCAAGTACAA CACTGCCACC 
TGCAGCACTG TGGTGGGCGA CGACGAGTGG GGCCACCTCC AGGTGGATGC CACCTCCCTC 
TTCCTCCTGT TTCTGGCCCA GATGACAGCC TCCGGCTTAC GTATTATTTT CACTCTTGAC 
GAGGTGGCCT TCATACAGAA TCTTGTTTTT TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGAATGT GGGAACGTGG AGATAAGACT AATCAGGGTA TTCCAGAATT GAATGCAAGC 
TCTGTAGGAA TGGCCAAGGC AGCCCTTGAA GCAATTGATG AACTGGACCT TTTTGGAGCC 
CATGGAGGTC GCAAGTCTGT GATCCACGTC CTGCCGGATG AGGTGGAACA CTGCCAGTCT 
ATTCTGTTCT CCATGCTGCC AAGAGCCTCG ACGTCAAAAG AGATTGATGC TGGGCTTCTT 
TCCATTATTT CTTTCCCGGC CTTCGCGGTG GAAGATGTGA ACCTTGTGAA TGTGACAAAA 
AATGAAATTA TTTCCAAGCT CCAGGGGCGT TATGGATGCT GTCGCTTCCT TCGAGATGGT 
TATAAAACCC CAAGAGAGGA CCCAAACCGA TTGCATTATG ACCCTGCTGA ACTCAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTCATAAT AGATGGAGTC 
TTCAATGGTG ATGCCGTTCA GGTCCAAGAA TACCGTGAAG CCCTGGAGGG GATACTGATC 
AGAGGCAATA ACGGGGTCCA CTTGTTGCCT GAACTCTACG CCATCCCACC TGACAAGGTG 
GATGAAGAGT ATAAGAATCC TCACACGGTG GATAGAGTTC CCCTGGGGAA GGTGCCGCAT 
CTGTGGGGTC AGTCCTTGTA CATCCTCAGC TCCCTGTTGG CAGAGGGATT CCTTGCTGCT 
GGTGAAATTG ATCCCTTGAA CAGAAGATTT TCCACTTCAG TCAAACCTGA TGTCGTCGTA 
CAAGTGACTG TTCTGGCAGA AAACAACCAC ATTAAGGAGC TTCTGAGGAA ACATGGGGTC 
AACGTCCAGA GTATCGCTGA CATTCATCCA ATCCGAGTCC AGCCAGGCCG CATCCTTAGT 
CACATATATG CCAAGCTTGG ACGGAATAAG AATATGAGAT TGAGTGGGCG ACCGTATCGG 
CACATTGGTG TCCTTGGGAC ATCGAAACTA TACGTGATTA GGAACCAGAT CTTTACTTTC 
ACGCCCCAGT TCACCGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATCGTA 
GAGATGCTGA GGATTGAGCT GGCCTACCTG TGCACCTGCT GGCGGATGAC GGGCAGACCC 
ACGCTCACCT TCCCCATCAC GCATACCATG CTCACAAATG ATGGCTCAGA TATTCATTCC 
GCTGTTCTTG CTACAATTCG CAAACTAGAG GATGGATATT TTGGAGGAGC TAGAGTAAAA 
TTAGGGAGTC TTTCGGAATT TCTCACCACC TCTTTCTACA CGTATCTGAC CTTCCTGGAT 
CCAGACTGTG ATGAGAAGTT GTTTGATGAT GCCAGTGAAG GGACCTTCAG TCCTGATAGT 
GATTCAGACC TGGGAGGATA TTTGGAAGAC ACCTATAATC AAGAAAGCCA GGACGAGCTT 
GACCAGTATA TCAACCACCT TCTGCAAAGC ACATCCTCGA AGTGCTATCT GCCACCTCTC 
TGTAAGAAGA CAGAGGACAA CCAGGTTTTC AGTGCTATCC ATTCCACTCG GGATATACTT 
TCTGTGATGG CAAAAGCAAA GGGCTTGGAA ATTCCATTTG TTCCCATGGC TTTGCCAACT 
AAAGTTCTAA GTGCCCACCG TAAATCGCTG AATCTCGTTG ATTCTCCTCA GCCACTCCTA 
AAAAAGGCCC CTGAAGATGA CTTCCAGTTT CCCAGAGATG ACCATGGCGA CGTGGACTGT 
GAGAAGCTGG TTGAGCAGTT GAAAGACTGT TCGAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTGT ATGTAATAAA GGGTCCCAGC TGGGACACAA ATCTCTCTGG ACAGCACGGG 
GTCACTGTTC ACAACCTCCT CAGTGAGCTC TATGCGAAGG CCGGCTTGAA CCAGGAGTGG 
GGCCTGATCC GCTACATCTC CGGCCTCCTC CGGAAGAAAG TGGAGGTCCT GGCTGAGGCG 
TGCACGGACC TGCTGTCACA CCAGAAGCAG CTCACAGTGG GCCTGCCCCC AGAACCCCGG 
GAGAAGACCA TCTCTGCGCC TCTTCCCCCC GAGGAGCTCA CGAAGCTCAT CTACGAAGCC 
AGCGGGCAGG ACATCAGCAT CGCCGTGCTC ACACAGGAGA TCGTGGTGTA CCTGGCGATG 
TACGTCAGGG CACAGCCCAG CCTCTTCGTG GAGATGCTCC GGCTCCGAAT TGGACTGATC 
ATTCAGGTGA TGGCCACGGA GCTGGCACGG AGCCTGAACT GTTCAGTGCG GCCCATTCAC 
TCCTCCACAC CTAGCCCTGC TGTCTCCATC CATGAGGTGG GCCACACTGG AGTCACCAAA 
ACCGAGAGGA GCGGCATCAA CAGGCTGAGG AGTGAGATGA AACAGATGAC TAGGCGGTTT 
AGTGCTGATG AACAGCTCAT TTCTGTGAGC CAGGCCGCAT CCAGCAGCGC GCGCTCCTCA 
AAGTCTGCGA GGTCCAGCAC CCCATCCTCA CCCACGGGCA CATCGTCATC AGACTCGGGC 
AGGCAGCACA TGGGCTGGGG GGAACGGCAG GGCCAGTGGC TGCGCAGGAG AAGGCTGGAC 
GGCGCCATCA ACAGGGTCCC CGTGGGATTC TACCAGAAGG TGTGGAAGAT CCTTCAGAAG 
TGCCATGGTC TGTCCATCGA CGGTTACGTG CTCCCGTCCT CGACCACGCA AGAGATGACC 
CCGCAGGAGA TCAAGTTTGC TGTCCACGTT GAGTCAGTGC TGAACCGAGT GCCACAACCC 
GAGTACCGGC AGCTGCTGGT GGAGGCCATC ATGGTGCTGA CGCTGCTCTC GGACACAGAG 
ATGAACAGCA TCGGGGGCAT CATCCATGTG GACCAGATCG TGCACATGGC CAACCAGCTG 
TTCCTACAAG ACCAGGTGTC AAGTGGAGCC ACGGACACGC TGGAGAAAGA CCAAGCCACA 
GGCATCTGCC ACTTCTTTTA CGACAGCGCT CCAAGCGGGG CTTATGGGAC GATGACCTAC 
CTAACGAAGG CGGTGGCTTC TCATTTGCAG GAACTGCTGC CCAATTCAGG TTGCCAGATG 
CAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA2 ( XM_024987971.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024843739.1 Bos taurus phosphorylase kinase regulatory subunit alpha 2 (PHKA2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa238365
Clone ID Related Accession (Same CDS sequence)	XM_024987971.1
Accession Version	XM_024987971.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCA GGAGCAATTC CGGGGTCCGC TTAGACGGAT ACGCGCGGCT GGTGCAGCAA 
ACAATCCTCT GTTACCAGAA TCCCATCACG GGGCTGATGT CAGCCAGTCA TGAGCAGAAG 
GACGCCTGGG TACGGGATAA CATCTACAGC ATCCTGGCCG TGTGGGGCCT GGGCATGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAAT 
GTGGTGAAAC TGATGCGAGG TCTTCTCCAG TGCATGATGA GACAGGTGGA TAAAGTGGAG 
AGGTTCAAGC ACACTCAGAG TACCGAGGAC AGCCTGCACG CCAAGTACAA CACTGCCACC 
TGCAGCACTG TGGTGGGCGA CGACGAGTGG GGCCACCTCC AGGTGGATGC CACCTCCCTC 
TTCCTCCTGT TTCTGGCCCA GATGACAGCC TCCGGCTTAC GTATTATTTT CACTCTTGAC 
GAGGTGGCCT TCATACAGAA TCTTGTTTTT TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGAATGT GGGAACGTGG AGATAAGACT AATCAGGGTA TTCCAGAATT GAATGCAAGC 
TCTGTAGGAA TGGCCAAGGC AGCCCTTGAA GCAATTGATG AACTGGACCT TTTTGGAGCC 
CATGGAGGTC GCAAGTCTGT GATCCACGTC CTGCCGGATG AGGTGGAACA CTGCCAGTCT 
ATTCTGTTCT CCATGCTGCC AAGAGCCTCG ACGTCAAAAG AGATTGATGC TGGGCTTCTT 
TCCATTATTT CTTTCCCGGC CTTCGCGGTG GAAGATGTGA ACCTTGTGAA TGTGACAAAA 
AATGAAATTA TTTCCAAGCT CCAGGGGCGT TATGGATGCT GTCGCTTCCT TCGAGATGGT 
TATAAAACCC CAAGAGAGGA CCCAAACCGA TTGCATTATG ACCCTGCTGA ACTCAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTCATAAT AGATGGAGTC 
TTCAATGGTG ATGCCGTTCA GGTCCAAGAA TACCGTGAAG CCCTGGAGGG GATACTGATC 
AGAGGCAATA ACGGGGTCCA CTTGTTGCCT GAACTCTACG CCATCCCACC TGACAAGGTG 
GATGAAGAGT ATAAGAATCC TCACACGGTG GATAGAGTTC CCCTGGGGAA GGTGCCGCAT 
CTGTGGGGTC AGTCCTTGTA CATCCTCAGC TCCCTGTTGG CAGAGGGATT CCTTGCTGCT 
GGTGAAATTG ATCCCTTGAA CAGAAGATTT TCCACTTCAG TCAAACCTGA TGTCGTCGTA 
CAAGTGACTG TTCTGGCAGA AAACAACCAC ATTAAGGAGC TTCTGAGGAA ACATGGGGTC 
AACGTCCAGA GTATCGCTGA CATTCATCCA ATCCGAGTCC AGCCAGGCCG CATCCTTAGT 
CACATATATG CCAAGCTTGG ACGGAATAAG AATATGAGAT TGAGTGGGCG ACCGTATCGG 
CACATTGGTG TCCTTGGGAC ATCGAAACTA TACGTGATTA GGAACCAGAT CTTTACTTTC 
ACGCCCCAGT TCACCGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATCGTA 
GAGATGCTGA GGATTGAGCT GGCCTACCTG TGCACCTGCT GGCGGATGAC GGGCAGACCC 
ACGCTCACCT TCCCCATCAC GCATACCATG CTCACAAATG ATGGCTCAGA TATTCATTCC 
GCTGTTCTTG CTACAATTCG CAAACTAGAG GATGGATATT TTGGAGGAGC TAGAGTAAAA 
TTAGGGAGTC TTTCGGAATT TCTCACCACC TCTTTCTACA CGTATCTGAC CTTCCTGGAT 
CCAGACTGTG ATGAGAAGTT GTTTGATGAT GCCAGTGAAG GGACCTTCAG TCCTGATAGT 
GATTCAGACC TGGGAGGATA TTTGGAAGAC ACCTATAATC AAGAAAGCCA GGACGAGCTT 
GACCAGTATA TCAACCACCT TCTGCAAAGC ACATCCTCGA AGTGCTATCT GCCACCTCTC 
TGTAAGAAGA CAGAGGACAA CCAGGTTTTC AGTGCTATCC ATTCCACTCG GGATATACTT 
TCTGTGATGG CAAAAGCAAA GGGCTTGGAA ATTCCATTTG TTCCCATGGC TTTGCCAACT 
AAAGTTCTAA GTGCCCACCG TAAATCGCTG AATCTCGTTG ATTCTCCTCA GCCACTCCTA 
AAAAAGGCCC CTGAAGATGA CTTCCAGTTT CCCAGAGATG ACCATGGCGA CGTGGACTGT 
GAGAAGCTGG TTGAGCAGTT GAAAGACTGT TCGAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTGT ATGTAATAAA GGGTCCCAGC TGGGACACAA ATCTCTCTGG ACAGCACGGG 
GTCACTGTTC ACAACCTCCT CAGTGAGCTC TATGCGAAGG CCGGCTTGAA CCAGGAGTGG 
GGCCTGATCC GCTACATCTC CGGCCTCCTC CGGAAGAAAG TGGAGGTCCT GGCTGAGGCG 
TGCACGGACC TGCTGTCACA CCAGAAGCAG CTCACAGTGG GCCTGCCCCC AGAACCCCGG 
GAGAAGACCA TCTCTGCGCC TCTTCCCCCC GAGGAGCTCA CGAAGCTCAT CTACGAAGCC 
AGCGGGCAGG ACATCAGCAT CGCCGTGCTC ACACAGGAGA TCGTGGTGTA CCTGGCGATG 
TACGTCAGGG CACAGCCCAG CCTCTTCGTG GAGATGCTCC GGCTCCGAAT TGGACTGATC 
ATTCAGGTGA TGGCCACGGA GCTGGCACGG AGCCTGAACT GTTCAGTGCG GCCCATTCAC 
TCCTCCACAC CTAGCCCTGC TGTCTCCATC CATGAGGTGG GCCACACTGG AGTCACCAAA 
ACCGAGAGGA GCGGCATCAA CAGGCTGAGG AGTGAGATGA AACAGAGGTC CAGCACCCCA 
TCCTCACCCA CGGGCACATC GTCATCAGAC TCGGGCAGGC AGCACATGGG CTGGGGGGAA 
CGGCAGGGCC AGTGGCTGCG CAGGAGAAGG CTGGACGGCG CCATCAACAG GGTCCCCGTG 
GGATTCTACC AGAAGGTGTG GAAGATCCTT CAGAAGTGCC ATGGTCTGTC CATCGACGGT 
TACGTGCTCC CGTCCTCGAC CACGCAAGAG ATGACCCCGC AGGAGATCAA GTTTGCTGTC 
CACGTTGAGT CAGTGCTGAA CCGAGTGCCA CAACCCGAGT ACCGGCAGCT GCTGGTGGAG 
GCCATCATGG TGCTGACGCT GCTCTCGGAC ACAGAGATGA ACAGCATCGG GGGCATCATC 
CATGTGGACC AGATCGTGCA CATGGCCAAC CAGCTGTTCC TACAAGACCA GGTGTCAAGT 
GGAGCCACGG ACACGCTGGA GAAAGACCAA GCCACAGGCA TCTGCCACTT CTTTTACGAC 
AGCGCTCCAA GCGGGGCTTA TGGGACGATG ACCTACCTAA CGAAGGCGGT GGCTTCTCAT 
TTGCAGGAAC TGCTGCCCAA TTCAGGTTGC CAGATGCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024843739.1
CDS	253..3774
Translation	MRSRSNSGVRLDGYARLVQQTILCYQNPITGLMSASHEQKDAWVRDNIYSILAVWGLGMAYRKNADRDEDKAKAYELEQNVVKLMRGLLQCMMRQVDKVERFKHTQSTEDSLHAKYNTATCSTVVGDDEWGHLQVDATSLFLLFLAQMTASGLRIIFTLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGMAKAALEAIDELDLFGAHGGRKSVIHVLPDEVEHCQSILFSMLPRASTSKEIDAGLLSIISFPAFAVEDVNLVNVTKNEIISKLQGRYGCCRFLRDGYKTPREDPNRLHYDPAELKLFENIECEWPVFWTYFIIDGVFNGDAVQVQEYREALEGILIRGNNGVHLLPELYAIPPDKVDEEYKNPHTVDRVPLGKVPHLWGQSLYILSSLLAEGFLAAGEIDPLNRRFSTSVKPDVVVQVTVLAENNHIKELLRKHGVNVQSIADIHPIRVQPGRILSHIYAKLGRNKNMRLSGRPYRHIGVLGTSKLYVIRNQIFTFTPQFTDQHHFYLALDNEMIVEMLRIELAYLCTCWRMTGRPTLTFPITHTMLTNDGSDIHSAVLATIRKLEDGYFGGARVKLGSLSEFLTTSFYTYLTFLDPDCDEKLFDDASEGTFSPDSDSDLGGYLEDTYNQESQDELDQYINHLLQSTSSKCYLPPLCKKTEDNQVFSAIHSTRDILSVMAKAKGLEIPFVPMALPTKVLSAHRKSLNLVDSPQPLLKKAPEDDFQFPRDDHGDVDCEKLVEQLKDCSNLQDQADILYILYVIKGPSWDTNLSGQHGVTVHNLLSELYAKAGLNQEWGLIRYISGLLRKKVEVLAEACTDLLSHQKQLTVGLPPEPREKTISAPLPPEELTKLIYEASGQDISIAVLTQEIVVYLAMYVRAQPSLFVEMLRLRIGLIIQVMATELARSLNCSVRPIHSSTPSPAVSIHEVGHTGVTKTERSGINRLRSEMKQRSSTPSSPTGTSSSDSGRQHMGWGERQGQWLRRRRLDGAINRVPVGFYQKVWKILQKCHGLSIDGYVLPSSTTQEMTPQEIKFAVHVESVLNRVPQPEYRQLLVEAIMVLTLLSDTEMNSIGGIIHVDQIVHMANQLFLQDQVSSGATDTLEKDQATGICHFFYDSAPSGAYGTMTYLTKAVASHLQELLPNSGCQMQ

Target ORF information:

RefSeq Version	XM_024987971.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphorylase kinase regulatory subunit alpha 2 (PHKA2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024987971.1

ORF Insert Sequence:

ATGAGGAGCA GGAGCAATTC CGGGGTCCGC TTAGACGGAT ACGCGCGGCT GGTGCAGCAA 
ACAATCCTCT GTTACCAGAA TCCCATCACG GGGCTGATGT CAGCCAGTCA TGAGCAGAAG 
GACGCCTGGG TACGGGATAA CATCTACAGC ATCCTGGCCG TGTGGGGCCT GGGCATGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAAT 
GTGGTGAAAC TGATGCGAGG TCTTCTCCAG TGCATGATGA GACAGGTGGA TAAAGTGGAG 
AGGTTCAAGC ACACTCAGAG TACCGAGGAC AGCCTGCACG CCAAGTACAA CACTGCCACC 
TGCAGCACTG TGGTGGGCGA CGACGAGTGG GGCCACCTCC AGGTGGATGC CACCTCCCTC 
TTCCTCCTGT TTCTGGCCCA GATGACAGCC TCCGGCTTAC GTATTATTTT CACTCTTGAC 
GAGGTGGCCT TCATACAGAA TCTTGTTTTT TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGAATGT GGGAACGTGG AGATAAGACT AATCAGGGTA TTCCAGAATT GAATGCAAGC 
TCTGTAGGAA TGGCCAAGGC AGCCCTTGAA GCAATTGATG AACTGGACCT TTTTGGAGCC 
CATGGAGGTC GCAAGTCTGT GATCCACGTC CTGCCGGATG AGGTGGAACA CTGCCAGTCT 
ATTCTGTTCT CCATGCTGCC AAGAGCCTCG ACGTCAAAAG AGATTGATGC TGGGCTTCTT 
TCCATTATTT CTTTCCCGGC CTTCGCGGTG GAAGATGTGA ACCTTGTGAA TGTGACAAAA 
AATGAAATTA TTTCCAAGCT CCAGGGGCGT TATGGATGCT GTCGCTTCCT TCGAGATGGT 
TATAAAACCC CAAGAGAGGA CCCAAACCGA TTGCATTATG ACCCTGCTGA ACTCAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTCATAAT AGATGGAGTC 
TTCAATGGTG ATGCCGTTCA GGTCCAAGAA TACCGTGAAG CCCTGGAGGG GATACTGATC 
AGAGGCAATA ACGGGGTCCA CTTGTTGCCT GAACTCTACG CCATCCCACC TGACAAGGTG 
GATGAAGAGT ATAAGAATCC TCACACGGTG GATAGAGTTC CCCTGGGGAA GGTGCCGCAT 
CTGTGGGGTC AGTCCTTGTA CATCCTCAGC TCCCTGTTGG CAGAGGGATT CCTTGCTGCT 
GGTGAAATTG ATCCCTTGAA CAGAAGATTT TCCACTTCAG TCAAACCTGA TGTCGTCGTA 
CAAGTGACTG TTCTGGCAGA AAACAACCAC ATTAAGGAGC TTCTGAGGAA ACATGGGGTC 
AACGTCCAGA GTATCGCTGA CATTCATCCA ATCCGAGTCC AGCCAGGCCG CATCCTTAGT 
CACATATATG CCAAGCTTGG ACGGAATAAG AATATGAGAT TGAGTGGGCG ACCGTATCGG 
CACATTGGTG TCCTTGGGAC ATCGAAACTA TACGTGATTA GGAACCAGAT CTTTACTTTC 
ACGCCCCAGT TCACCGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATCGTA 
GAGATGCTGA GGATTGAGCT GGCCTACCTG TGCACCTGCT GGCGGATGAC GGGCAGACCC 
ACGCTCACCT TCCCCATCAC GCATACCATG CTCACAAATG ATGGCTCAGA TATTCATTCC 
GCTGTTCTTG CTACAATTCG CAAACTAGAG GATGGATATT TTGGAGGAGC TAGAGTAAAA 
TTAGGGAGTC TTTCGGAATT TCTCACCACC TCTTTCTACA CGTATCTGAC CTTCCTGGAT 
CCAGACTGTG ATGAGAAGTT GTTTGATGAT GCCAGTGAAG GGACCTTCAG TCCTGATAGT 
GATTCAGACC TGGGAGGATA TTTGGAAGAC ACCTATAATC AAGAAAGCCA GGACGAGCTT 
GACCAGTATA TCAACCACCT TCTGCAAAGC ACATCCTCGA AGTGCTATCT GCCACCTCTC 
TGTAAGAAGA CAGAGGACAA CCAGGTTTTC AGTGCTATCC ATTCCACTCG GGATATACTT 
TCTGTGATGG CAAAAGCAAA GGGCTTGGAA ATTCCATTTG TTCCCATGGC TTTGCCAACT 
AAAGTTCTAA GTGCCCACCG TAAATCGCTG AATCTCGTTG ATTCTCCTCA GCCACTCCTA 
AAAAAGGCCC CTGAAGATGA CTTCCAGTTT CCCAGAGATG ACCATGGCGA CGTGGACTGT 
GAGAAGCTGG TTGAGCAGTT GAAAGACTGT TCGAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTGT ATGTAATAAA GGGTCCCAGC TGGGACACAA ATCTCTCTGG ACAGCACGGG 
GTCACTGTTC ACAACCTCCT CAGTGAGCTC TATGCGAAGG CCGGCTTGAA CCAGGAGTGG 
GGCCTGATCC GCTACATCTC CGGCCTCCTC CGGAAGAAAG TGGAGGTCCT GGCTGAGGCG 
TGCACGGACC TGCTGTCACA CCAGAAGCAG CTCACAGTGG GCCTGCCCCC AGAACCCCGG 
GAGAAGACCA TCTCTGCGCC TCTTCCCCCC GAGGAGCTCA CGAAGCTCAT CTACGAAGCC 
AGCGGGCAGG ACATCAGCAT CGCCGTGCTC ACACAGGAGA TCGTGGTGTA CCTGGCGATG 
TACGTCAGGG CACAGCCCAG CCTCTTCGTG GAGATGCTCC GGCTCCGAAT TGGACTGATC 
ATTCAGGTGA TGGCCACGGA GCTGGCACGG AGCCTGAACT GTTCAGTGCG GCCCATTCAC 
TCCTCCACAC CTAGCCCTGC TGTCTCCATC CATGAGGTGG GCCACACTGG AGTCACCAAA 
ACCGAGAGGA GCGGCATCAA CAGGCTGAGG AGTGAGATGA AACAGAGGTC CAGCACCCCA 
TCCTCACCCA CGGGCACATC GTCATCAGAC TCGGGCAGGC AGCACATGGG CTGGGGGGAA 
CGGCAGGGCC AGTGGCTGCG CAGGAGAAGG CTGGACGGCG CCATCAACAG GGTCCCCGTG 
GGATTCTACC AGAAGGTGTG GAAGATCCTT CAGAAGTGCC ATGGTCTGTC CATCGACGGT 
TACGTGCTCC CGTCCTCGAC CACGCAAGAG ATGACCCCGC AGGAGATCAA GTTTGCTGTC 
CACGTTGAGT CAGTGCTGAA CCGAGTGCCA CAACCCGAGT ACCGGCAGCT GCTGGTGGAG 
GCCATCATGG TGCTGACGCT GCTCTCGGAC ACAGAGATGA ACAGCATCGG GGGCATCATC 
CATGTGGACC AGATCGTGCA CATGGCCAAC CAGCTGTTCC TACAAGACCA GGTGTCAAGT 
GGAGCCACGG ACACGCTGGA GAAAGACCAA GCCACAGGCA TCTGCCACTT CTTTTACGAC 
AGCGCTCCAA GCGGGGCTTA TGGGACGATG ACCTACCTAA CGAAGGCGGT GGCTTCTCAT 
TTGCAGGAAC TGCTGCCCAA TTCAGGTTGC CAGATGCAGT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA2 ( NM_001191545.1 ) cDNA ORF clone, Bos taurus(cattle) -> NP_001178474.1 Bos taurus phosphorylase kinase regulatory subunit alpha 2 (PHKA2), mRNA.

Price & Availability↑

CloneID	OBa35760
Clone ID Related Accession (Same CDS sequence)	NM_001191545.1 , XM_005228391.2 , XM_024987966.1
Accession Version	NM_001191545.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-06-02
Organism	Bos taurus(cattle)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from NKLS02000030.1. On Apr 16, 2011 this sequence version replaced XM_002707412.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: single sample supports all introns SAMN03145413, SAMN03145415 [ECO:0000348] ##Evidence-Data-END##

ATGAGGAGCA GGAGCAATTC CGGGGTCCGC TTAGACGGAT ACGCGCGGCT GGTGCAGCAA 
ACAATCCTCT GTTACCAGAA TCCCATCACG GGGCTGATGT CAGCCAGTCA TGAGCAGAAG 
GACGCCTGGG TACGGGATAA CATCTACAGC ATCCTGGCCG TGTGGGGCCT GGGCATGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAAT 
GTGGTGAAAC TGATGCGAGG TCTTCTCCAG TGCATGATGA GACAGGTGGA TAAAGTGGAG 
AGGTTCAAGC ACACTCAGAG TACCGAGGAC AGCCTGCACG CCAAGTACAA CACTGCCACC 
TGCAGCACTG TGGTGGGCGA CGACGAGTGG GGCCACCTCC AGGTGGATGC CACCTCCCTC 
TTCCTCCTGT TTCTGGCCCA GATGACAGCC TCCGGCTTAC GTATTATTTT CACTCTTGAC 
GAGGTGGCCT TCATACAGAA TCTTGTTTTT TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGAATGT GGGAACGTGG AGATAAGACT AATCAGGGTA TTCCAGAATT GAATGCAAGC 
TCTGTAGGAA TGGCCAAGGC AGCCCTTGAA GCAATTGATG AACTGGACCT TTTTGGAGCC 
CATGGAGGTC GCAAGTCTGT GATCCACGTC CTGCCGGATG AGGTGGAACA CTGCCAGTCT 
ATTCTGTTCT CCATGCTGCC AAGAGCCTCG ACGTCAAAAG AGATTGATGC TGGGCTTCTT 
TCCATTATTT CTTTCCCGGC CTTCGCGGTG GAAGATGTGA ACCTTGTGAA TGTGACAAAA 
AATGAAATTA TTTCCAAGCT CCAGGGGCGT TATGGATGCT GTCGCTTCCT TCGAGATGGT 
TATAAAACCC CAAGAGAGGA CCCAAACCGA TTGCATTATG ACCCTGCTGA ACTCAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTCATAAT AGATGGAGTC 
TTCAATGGTG ATGCCGTTCA GGTCCAAGAA TACCGTGAAG CCCTGGAGGG GATACTGATC 
AGAGGCAATA ACGGGGTCCA CTTGTTGCCT GAACTCTACG CCATCCCACC TGACAAGGTG 
GATGAAGAGT ATAAGAATCC TCACACGGTG GATAGAGTTC CCCTGGGGAA GGTGCCGCAT 
CTGTGGGGTC AGTCCTTGTA CATCCTCAGC TCCCTGTTGG CAGAGGGATT CCTTGCTGCT 
GGTGAAATTG ATCCCTTGAA CAGAAGATTT TCCACTTCAG TCAAACCTGA TGTCGTCGTA 
CAAGTGACTG TTCTGGCAGA AAACAACCAC ATTAAGGAGC TTCTGAGGAA ACATGGGGTC 
AACGTCCAGA GTATCGCTGA CATTCATCCA ATCCGAGTCC AGCCAGGCCG CATCCTTAGT 
CACATATATG CCAAGCTTGG ACGGAATAAG AATATGAGAT TGAGTGGGCG ACCGTATCGG 
CACATTGGTG TCCTTGGGAC ATCGAAACTA TACGTGATTA GGAACCAGAT CTTTACTTTC 
ACGCCCCAGT TCACCGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATCGTA 
GAGATGCTGA GGATTGAGCT GGCCTACCTG TGCACCTGCT GGCGGATGAC GGGCAGACCC 
ACGCTCACCT TCCCCATCAC GCATACCATG CTCACAAATG ATGGCTCAGA TATTCATTCC 
GCTGTTCTTG CTACAATTCG CAAACTAGAG GATGGATATT TTGGAGGAGC TAGAGTAAAA 
TTAGGGAGTC TTTCGGAATT TCTCACCACC TCTTTCTACA CGTATCTGAC CTTCCTGGAT 
CCAGACTGTG ATGAGAAGTT GTTTGATGAT GCCAGTGAAG GGACCTTCAG TCCTGATAGT 
GATTCAGACC TGGGAGGATA TTTGGAAGAC ACCTATAATC AAGAAAGCCA GGACGAGCTT 
GACCAGTATA TCAACCACCT TCTGCAAAGC ACATCCTCGA AGTGCTATCT GCCACCTCTC 
TGTAAGAAGA CAGAGGACAA CCAGGTTTTC AGTGCTATCC ATTCCACTCG GGATATACTT 
TCTGTGATGG CAAAAGCAAA GGGCTTGGAA ATTCCATTTG TTCCCATGGC TTTGCCAACT 
AAAGTTCTAA GTGCCCACCG TAAATCGCTG AATCTCGTTG ATTCTCCTCA GCCACTCCTA 
AAAAAGGCCC CTGAAGATGA CTTCCAGTTT CCCAGAGATG ACCATGGCGA CGTGGACTGT 
GAGAAGCTGG TTGAGCAGTT GAAAGACTGT TCGAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTGT ATGTAATAAA GGGTCCCAGC TGGGACACAA ATCTCTCTGG ACAGCACGGG 
GTCACTGTTC ACAACCTCCT CAGTGAGCTC TATGCGAAGG CCGGCTTGAA CCAGGAGTGG 
GGCCTGATCC GCTACATCTC CGGCCTCCTC CGGAAGAAAG TGGAGGTCCT GGCTGAGGCG 
TGCACGGACC TGCTGTCACA CCAGAAGCAG CTCACAGTGG GCCTGCCCCC AGAACCCCGG 
GAGAAGACCA TCTCTGCGCC TCTTCCCCCC GAGGAGCTCA CGAAGCTCAT CTACGAAGCC 
AGCGGGCAGG ACATCAGCAT CGCCGTGCTC ACACAGGAGA TCGTGGTGTA CCTGGCGATG 
TACGTCAGGG CACAGCCCAG CCTCTTCGTG GAGATGCTCC GGCTCCGAAT TGGACTGATC 
ATTCAGGTGA TGGCCACGGA GCTGGCACGG AGCCTGAACT GTTCAGGAGA AGAAGCTTCT 
GAAAGTCTGA TGAACCTCAG CCCTTTTGAT ATGAAAAATC TCCTGCACCA CATCTTAAGT 
GGCAAAGAAT TTGGTGTTGA AAGAAGCATG CGGCCCATTC ACTCCTCCAC ACCTAGCCCT 
GCTGTCTCCA TCCATGAGGT GGGCCACACT GGAGTCACCA AAACCGAGAG GAGCGGCATC 
AACAGGCTGA GGAGTGAGAT GAAACAGATG ACTAGGCGGT TTAGTGCTGA TGAACAGCTC 
ATTTCTGTGA GCCAGGCCGC ATCCAGCAGC GCGCGCTCCT CAAAGTCTGC GAGGTCCAGC 
ACCCCATCCT CACCCACGGG CACATCGTCA TCAGACTCGG GCAGGCAGCA CATGGGCTGG 
GGGGAACGGC AGGGCCAGTG GCTGCGCAGG AGAAGGCTGG ACGGCGCCAT CAACAGGGTC 
CCCGTGGGAT TCTACCAGAA GGTGTGGAAG ATCCTTCAGA AGTGCCATGG TCTGTCCATC 
GACGGTTACG TGCTCCCGTC CTCGACCACG CAAGAGATGA CCCCGCAGGA GATCAAGTTT 
GCTGTCCACG TTGAGTCAGT GCTGAACCGA GTGCCACAAC CCGAGTACCG GCAGCTGCTG 
GTGGAGGCCA TCATGGTGCT GACGCTGCTC TCGGACACAG AGATGAACAG CATCGGGGGC 
ATCATCCATG TGGACCAGAT CGTGCACATG GCCAACCAGC TGTTCCTACA AGACCAGGTG 
TCAAGTGGAG CCACGGACAC GCTGGAGAAA GACCAAGCCA CAGGCATCTG CCACTTCTTT 
TACGACAGCG CTCCAAGCGG GGCTTATGGG ACGATGACCT ACCTAACGAA GGCGGTGGCT 
TCTCATTTGC AGGAACTGCT GCCCAATTCA GGTTGCCAGA TGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001178474.1
CDS	48..3755
Translation	MRSRSNSGVRLDGYARLVQQTILCYQNPITGLMSASHEQKDAWVRDNIYSILAVWGLGMAYRKNADRDEDKAKAYELEQNVVKLMRGLLQCMMRQVDKVERFKHTQSTEDSLHAKYNTATCSTVVGDDEWGHLQVDATSLFLLFLAQMTASGLRIIFTLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGMAKAALEAIDELDLFGAHGGRKSVIHVLPDEVEHCQSILFSMLPRASTSKEIDAGLLSIISFPAFAVEDVNLVNVTKNEIISKLQGRYGCCRFLRDGYKTPREDPNRLHYDPAELKLFENIECEWPVFWTYFIIDGVFNGDAVQVQEYREALEGILIRGNNGVHLLPELYAIPPDKVDEEYKNPHTVDRVPLGKVPHLWGQSLYILSSLLAEGFLAAGEIDPLNRRFSTSVKPDVVVQVTVLAENNHIKELLRKHGVNVQSIADIHPIRVQPGRILSHIYAKLGRNKNMRLSGRPYRHIGVLGTSKLYVIRNQIFTFTPQFTDQHHFYLALDNEMIVEMLRIELAYLCTCWRMTGRPTLTFPITHTMLTNDGSDIHSAVLATIRKLEDGYFGGARVKLGSLSEFLTTSFYTYLTFLDPDCDEKLFDDASEGTFSPDSDSDLGGYLEDTYNQESQDELDQYINHLLQSTSSKCYLPPLCKKTEDNQVFSAIHSTRDILSVMAKAKGLEIPFVPMALPTKVLSAHRKSLNLVDSPQPLLKKAPEDDFQFPRDDHGDVDCEKLVEQLKDCSNLQDQADILYILYVIKGPSWDTNLSGQHGVTVHNLLSELYAKAGLNQEWGLIRYISGLLRKKVEVLAEACTDLLSHQKQLTVGLPPEPREKTISAPLPPEELTKLIYEASGQDISIAVLTQEIVVYLAMYVRAQPSLFVEMLRLRIGLIIQVMATELARSLNCSGEEASESLMNLSPFDMKNLLHHILSGKEFGVERSMRPIHSSTPSPAVSIHEVGHTGVTKTERSGINRLRSEMKQMTRRFSADEQLISVSQAASSSARSSKSARSSTPSSPTGTSSSDSGRQHMGWGERQGQWLRRRRLDGAINRVPVGFYQKVWKILQKCHGLSIDGYVLPSSTTQEMTPQEIKFAVHVESVLNRVPQPEYRQLLVEAIMVLTLLSDTEMNSIGGIIHVDQIVHMANQLFLQDQVSSGATDTLEKDQATGICHFFYDSAPSGAYGTMTYLTKAVASHLQELLPNSGCQMQ

Target ORF information:

RefSeq Version	NM_001191545.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphorylase kinase regulatory subunit alpha 2 (PHKA2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001191545.1

ORF Insert Sequence:

ATGAGGAGCA GGAGCAATTC CGGGGTCCGC TTAGACGGAT ACGCGCGGCT GGTGCAGCAA 
ACAATCCTCT GTTACCAGAA TCCCATCACG GGGCTGATGT CAGCCAGTCA TGAGCAGAAG 
GACGCCTGGG TACGGGATAA CATCTACAGC ATCCTGGCCG TGTGGGGCCT GGGCATGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAAT 
GTGGTGAAAC TGATGCGAGG TCTTCTCCAG TGCATGATGA GACAGGTGGA TAAAGTGGAG 
AGGTTCAAGC ACACTCAGAG TACCGAGGAC AGCCTGCACG CCAAGTACAA CACTGCCACC 
TGCAGCACTG TGGTGGGCGA CGACGAGTGG GGCCACCTCC AGGTGGATGC CACCTCCCTC 
TTCCTCCTGT TTCTGGCCCA GATGACAGCC TCCGGCTTAC GTATTATTTT CACTCTTGAC 
GAGGTGGCCT TCATACAGAA TCTTGTTTTT TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGAATGT GGGAACGTGG AGATAAGACT AATCAGGGTA TTCCAGAATT GAATGCAAGC 
TCTGTAGGAA TGGCCAAGGC AGCCCTTGAA GCAATTGATG AACTGGACCT TTTTGGAGCC 
CATGGAGGTC GCAAGTCTGT GATCCACGTC CTGCCGGATG AGGTGGAACA CTGCCAGTCT 
ATTCTGTTCT CCATGCTGCC AAGAGCCTCG ACGTCAAAAG AGATTGATGC TGGGCTTCTT 
TCCATTATTT CTTTCCCGGC CTTCGCGGTG GAAGATGTGA ACCTTGTGAA TGTGACAAAA 
AATGAAATTA TTTCCAAGCT CCAGGGGCGT TATGGATGCT GTCGCTTCCT TCGAGATGGT 
TATAAAACCC CAAGAGAGGA CCCAAACCGA TTGCATTATG ACCCTGCTGA ACTCAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTCATAAT AGATGGAGTC 
TTCAATGGTG ATGCCGTTCA GGTCCAAGAA TACCGTGAAG CCCTGGAGGG GATACTGATC 
AGAGGCAATA ACGGGGTCCA CTTGTTGCCT GAACTCTACG CCATCCCACC TGACAAGGTG 
GATGAAGAGT ATAAGAATCC TCACACGGTG GATAGAGTTC CCCTGGGGAA GGTGCCGCAT 
CTGTGGGGTC AGTCCTTGTA CATCCTCAGC TCCCTGTTGG CAGAGGGATT CCTTGCTGCT 
GGTGAAATTG ATCCCTTGAA CAGAAGATTT TCCACTTCAG TCAAACCTGA TGTCGTCGTA 
CAAGTGACTG TTCTGGCAGA AAACAACCAC ATTAAGGAGC TTCTGAGGAA ACATGGGGTC 
AACGTCCAGA GTATCGCTGA CATTCATCCA ATCCGAGTCC AGCCAGGCCG CATCCTTAGT 
CACATATATG CCAAGCTTGG ACGGAATAAG AATATGAGAT TGAGTGGGCG ACCGTATCGG 
CACATTGGTG TCCTTGGGAC ATCGAAACTA TACGTGATTA GGAACCAGAT CTTTACTTTC 
ACGCCCCAGT TCACCGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATCGTA 
GAGATGCTGA GGATTGAGCT GGCCTACCTG TGCACCTGCT GGCGGATGAC GGGCAGACCC 
ACGCTCACCT TCCCCATCAC GCATACCATG CTCACAAATG ATGGCTCAGA TATTCATTCC 
GCTGTTCTTG CTACAATTCG CAAACTAGAG GATGGATATT TTGGAGGAGC TAGAGTAAAA 
TTAGGGAGTC TTTCGGAATT TCTCACCACC TCTTTCTACA CGTATCTGAC CTTCCTGGAT 
CCAGACTGTG ATGAGAAGTT GTTTGATGAT GCCAGTGAAG GGACCTTCAG TCCTGATAGT 
GATTCAGACC TGGGAGGATA TTTGGAAGAC ACCTATAATC AAGAAAGCCA GGACGAGCTT 
GACCAGTATA TCAACCACCT TCTGCAAAGC ACATCCTCGA AGTGCTATCT GCCACCTCTC 
TGTAAGAAGA CAGAGGACAA CCAGGTTTTC AGTGCTATCC ATTCCACTCG GGATATACTT 
TCTGTGATGG CAAAAGCAAA GGGCTTGGAA ATTCCATTTG TTCCCATGGC TTTGCCAACT 
AAAGTTCTAA GTGCCCACCG TAAATCGCTG AATCTCGTTG ATTCTCCTCA GCCACTCCTA 
AAAAAGGCCC CTGAAGATGA CTTCCAGTTT CCCAGAGATG ACCATGGCGA CGTGGACTGT 
GAGAAGCTGG TTGAGCAGTT GAAAGACTGT TCGAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTGT ATGTAATAAA GGGTCCCAGC TGGGACACAA ATCTCTCTGG ACAGCACGGG 
GTCACTGTTC ACAACCTCCT CAGTGAGCTC TATGCGAAGG CCGGCTTGAA CCAGGAGTGG 
GGCCTGATCC GCTACATCTC CGGCCTCCTC CGGAAGAAAG TGGAGGTCCT GGCTGAGGCG 
TGCACGGACC TGCTGTCACA CCAGAAGCAG CTCACAGTGG GCCTGCCCCC AGAACCCCGG 
GAGAAGACCA TCTCTGCGCC TCTTCCCCCC GAGGAGCTCA CGAAGCTCAT CTACGAAGCC 
AGCGGGCAGG ACATCAGCAT CGCCGTGCTC ACACAGGAGA TCGTGGTGTA CCTGGCGATG 
TACGTCAGGG CACAGCCCAG CCTCTTCGTG GAGATGCTCC GGCTCCGAAT TGGACTGATC 
ATTCAGGTGA TGGCCACGGA GCTGGCACGG AGCCTGAACT GTTCAGGAGA AGAAGCTTCT 
GAAAGTCTGA TGAACCTCAG CCCTTTTGAT ATGAAAAATC TCCTGCACCA CATCTTAAGT 
GGCAAAGAAT TTGGTGTTGA AAGAAGCATG CGGCCCATTC ACTCCTCCAC ACCTAGCCCT 
GCTGTCTCCA TCCATGAGGT GGGCCACACT GGAGTCACCA AAACCGAGAG GAGCGGCATC 
AACAGGCTGA GGAGTGAGAT GAAACAGATG ACTAGGCGGT TTAGTGCTGA TGAACAGCTC 
ATTTCTGTGA GCCAGGCCGC ATCCAGCAGC GCGCGCTCCT CAAAGTCTGC GAGGTCCAGC 
ACCCCATCCT CACCCACGGG CACATCGTCA TCAGACTCGG GCAGGCAGCA CATGGGCTGG 
GGGGAACGGC AGGGCCAGTG GCTGCGCAGG AGAAGGCTGG ACGGCGCCAT CAACAGGGTC 
CCCGTGGGAT TCTACCAGAA GGTGTGGAAG ATCCTTCAGA AGTGCCATGG TCTGTCCATC 
GACGGTTACG TGCTCCCGTC CTCGACCACG CAAGAGATGA CCCCGCAGGA GATCAAGTTT 
GCTGTCCACG TTGAGTCAGT GCTGAACCGA GTGCCACAAC CCGAGTACCG GCAGCTGCTG 
GTGGAGGCCA TCATGGTGCT GACGCTGCTC TCGGACACAG AGATGAACAG CATCGGGGGC 
ATCATCCATG TGGACCAGAT CGTGCACATG GCCAACCAGC TGTTCCTACA AGACCAGGTG 
TCAAGTGGAG CCACGGACAC GCTGGAGAAA GACCAAGCCA CAGGCATCTG CCACTTCTTT 
TACGACAGCG CTCCAAGCGG GGCTTATGGG ACGATGACCT ACCTAACGAA GGCGGTGGCT 
TCTCATTTGC AGGAACTGCT GCCCAATTCA GGTTGCCAGA TGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA2 ( XM_010822157.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_010820459.1 Bos taurus phosphorylase kinase, alpha 2 (liver) (PHKA2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	OBa35759
Clone ID Related Accession (Same CDS sequence)	XM_010822157.2 , XM_005228390.3
Accession Version	XM_010822157.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104772.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_010822157.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCA GGAGCAATTC CGGGGTCCGC TTAGACGGAT ACGCGCGGCT GGTGCAGCAA 
ACAATCCTCT GTTACCAGAA TCCCATCACG GGGCTGATGT CAGCCAGTCA TGAGCAGAAG 
GACGCCTGGG TACGGGATAA CATCTACAGC ATCCTGGCCG TGTGGGGCCT GGGCATGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAAT 
GTGGTGAAAC TGATGCGAGG TCTTCTCCAG TGCATGATGA GACAGGTGGA TAAAGTGGAG 
AGGTTCAAGC ACACTCAGAG TACCGAGGAC AGCCTGCACG CCAAGTACAA CACTGCCACC 
TGCAGCACTG TGGTGGGCGA CGACGAGTGG GGCCACCTCC AGGTGGATGC CACCTCCCTC 
TTCCTCCTGT TTCTGGCCCA GATGACAGCC TCCGGCTTAC GTATTATTTT CACTCTTGAC 
GAGGTGGCCT TCATACAGAA TCTTGTTTTT TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGAATGT GGGAACGTGG AGATAAGACT AATCAGGGTA TTCCAGAATT GAATGCAAGC 
TCTGTAGGAA TGGCCAAGGC AGCCCTTGAA GCAATTGATG AACTGGACCT TTTTGGAGCC 
CATGGAGGTC GCAAGTCTGT GATCCACGTC CTGCCGGATG AGGTGGAACA CTGCCAGTCT 
ATTCTGTTCT CCATGCTGCC AAGAGCCTCG ACGTCAAAAG AGATTGATGC TGGGCTTCTT 
TCCATTATTT CTTTCCCGGC CTTCGCGGTG GAAGATGTGA ACCTTGTGAA TGTGACAAAA 
AATGAAATTA TTTCCAAGCT CCAGGGGCGT TATGGATGCT GTCGCTTCCT TCGAGATGGT 
TATAAAACCC CAAGAGAGGA CCCAAACCGA TTGCATTATG ACCCTGCTGA ACTCAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTCATAAT AGATGGAGTC 
TTCAATGGTG ATGCCGTTCA GGTCCAAGAA TACCGTGAAG CCCTGGAGGG GATACTGATC 
AGAGGCAATA ACGGGGTCCA CTTGTTGCCT GAACTCTACG CCATCCCACC TGACAAGGTG 
GATGAAGAGT ATAAGAATCC TCACACGGTG GATAGAGTTC CCCTGGGGAA GGTGCCGCAT 
CTGTGGGGTC AGTCCTTGTA CATCCTCAGC TCCCTGTTGG CAGAGGGATT CCTTGCTGCT 
GGTGAAATTG ATCCCTTGAA CAGAAGATTT TCCACTTCAG TCAAACCTGA TGTCGTCGTA 
CAAGTGACTG TTCTGGCAGA AAACAACCAC ATTAAGGAGC TTCTGAGGAA ACATGGGGTC 
AACGTCCAGA GTATCGCTGA CATTCATCCA ATCCGAGTCC AGCCAGGCCG CATCCTTAGT 
CACATATATG CCAAGCTTGG ACGGAATAAG AATATGAGAT TGAGTGGGCG ACCGTATCGG 
CACATTGGTG TCCTTGGGAC ATCGAAACTA TACGTGATTA GGAACCAGAT CTTTACTTTC 
ACGCCCCAGT TCACCGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATCGTA 
GAGATGCTGA GGATTGAGCT GGCCTACCTG TGCACCTGCT GGCGGATGAC GGGCAGACCC 
ACGCTCACCT TCCCCATCAC GCATACCATG CTCACAAATG ATGGCTCAGA TATTCATTCC 
GCTGTTCTTG CTACAATTCG CAAACTAGAG GATGGATATT TTGGAGGAGC TAGAGTAAAA 
TTAGGGAGTC TTTCGGAATT TCTCACCACC TCTTTCTACA CGTATCTGAC CTTCCTGGAT 
CCAGACTGTG ATGAGAAGTT GTTTGATGAT GCCAGTGAAG GGACCTTCAG TCCTGATAGT 
GATTCAGACC TGGGAGGATA TTTGGAAGAC ACCTATAATC AAGAAAGCCA GGACGAGCTT 
GACCAGTATA TCAACCACCT TCTGCAAAGC ACATCCTCGA AGTGCTATCT GCCACCTCTC 
TGTAAGAAGA CAGAGGACAA CCAGGTTTTC AGTGCTATCC ATTCCACTCG GGATATACTT 
TCTGTGATGG CAAAAGCAAA GGGCTTGGAA ATTCCATTTG TTCCCATGGC TTTGCCAACT 
AAAGTTCTAA GTGCCCACCG TAAATCGCTG AATCTCGTTG ATTCTCCTCA GCCACTCCTA 
AAAAAGGCCC CTGAAGATGA CTTCCAGTTT CCCAGAGATG ACCATGGCGA CGTGGACTGT 
GAGAAGCTGG TTGAGCAGTT GAAAGACTGT TCGAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTGT ATGTAATAAA GGGTCCCAGC TGGGACACAA ATCTCTCTGG ACAGCACGGG 
GTCACTGTTC ACAACCTCCT CAGTGAGCTC TATGCGAAGG CCGGCTTGAA CCAGGAGTGG 
GGCCTGATCC GCTACATCTC CGGCCTCCTC CGGAAGAAAG TGGAGGTCCT GGCTGAGGCG 
TGCACGGACC TGCTGTCACA CCAGAAGCAG CTCACAGTGG GCCTGCCCCC AGAACCCCGG 
GAGAAGACCA TCTCTGCGCC TCTTCCCCCC GAGGAGCTCA CGAAGCTCAT CTACGAAGCC 
AGCGGGCAGG ACATCAGCAT CGCCGTGCTC ACACAGGAGA TCGTGGTGTA CCTGGCGATG 
TACGTCAGGG CACAGCCCAG CCTCTTCGTG GAGATGCTCC GGCTCCGAAT TGGACTGATC 
ATTCAGGTGA TGGCCACGGA GCTGGCACGG AGCCTGAACT GTTCAGGAGA AGAAGCTTCT 
GAAAGTCTGA TGAACCTCAG CCCTTTTGAT ATGAAAAATC TCCTGCACCA CATCTTAAGT 
GGCAAAGAAT TTGGTGTTGA AAGAAGCATG CGGCCCATTC ACTCCTCCAC ACCTAGCCCT 
GCTGTCTCCA TCCATGAGGT GGGCCACACT GGAGTCACCA AAACCGAGAG GAGCGGCATC 
AACAGGCTGA GGAGTGAGAT GAAACAGATG ACTAGGCGGT TTAGTGCTGA TGAACAGCTC 
ATTTCTGTGA GCCAGGCCGC ATCCAGCAGC GCGCGCTCCT CAAAGTCTGC GAGGTCCAGC 
ACCCCATCCT CACCCACGGG CACATCGTCA TCAGACTCGG GCAGGCAGCA CATGGGCTGG 
GGGGAACGGC AGGGCCAGTG GCTGCGCAGG AGAAGGCTGG ACGGCGCCAT CAACAGGGTC 
CCCGTGGGAT TCTACCAGAA GGTGTGGAAG ATCCTTCAGA AGTGCCATGG TCTGTCCATC 
GACGGTTACG TGCTCCCGTC CTCGACCACG CAAGAGATGA CCCCGCAGGA GATCAAGTTT 
GCTGTCCACG TTGAGTCAGT GCTGAACCGA GTGCCACAAC CCGAGTACCG GCAGCTGCTG 
GTGGAGGCCA TCATGGTGCT GACGCTGCTC TCGGACACAG AGATGAACAG CATCGGGGGC 
ATCATCCATG TGGACCAGAT CGTGCACATG GCCAACCAGC TGTTCCTACA AGACCAGGTG 
TCAAGTGGAG CCACGGACAC GCTGGAGAAA GACCAAGCCA CAGGCATCTG CCACTTCTTT 
TACGACAGCG CTCCAAGCGG GGCTTATGGG ACGATGACCT ACCTAACGAA GGCGGTGGCT 
TCTCATTTGC AGGAACTGCT GCCCAATTCA GCATCTGGTC TCCTGGAGTC TGAGGACAGA 
TTGGAAGTGG GTCTCTCAAG TGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_010820459.1
CDS	349..4098
Translation	MRSRSNSGVRLDGYARLVQQTILCYQNPITGLMSASHEQKDAWVRDNIYSILAVWGLGMAYRKNADRDEDKAKAYELEQNVVKLMRGLLQCMMRQVDKVERFKHTQSTEDSLHAKYNTATCSTVVGDDEWGHLQVDATSLFLLFLAQMTASGLRIIFTLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGMAKAALEAIDELDLFGAHGGRKSVIHVLPDEVEHCQSILFSMLPRASTSKEIDAGLLSIISFPAFAVEDVNLVNVTKNEIISKLQGRYGCCRFLRDGYKTPREDPNRLHYDPAELKLFENIECEWPVFWTYFIIDGVFNGDAVQVQEYREALEGILIRGNNGVHLLPELYAIPPDKVDEEYKNPHTVDRVPLGKVPHLWGQSLYILSSLLAEGFLAAGEIDPLNRRFSTSVKPDVVVQVTVLAENNHIKELLRKHGVNVQSIADIHPIRVQPGRILSHIYAKLGRNKNMRLSGRPYRHIGVLGTSKLYVIRNQIFTFTPQFTDQHHFYLALDNEMIVEMLRIELAYLCTCWRMTGRPTLTFPITHTMLTNDGSDIHSAVLATIRKLEDGYFGGARVKLGSLSEFLTTSFYTYLTFLDPDCDEKLFDDASEGTFSPDSDSDLGGYLEDTYNQESQDELDQYINHLLQSTSSKCYLPPLCKKTEDNQVFSAIHSTRDILSVMAKAKGLEIPFVPMALPTKVLSAHRKSLNLVDSPQPLLKKAPEDDFQFPRDDHGDVDCEKLVEQLKDCSNLQDQADILYILYVIKGPSWDTNLSGQHGVTVHNLLSELYAKAGLNQEWGLIRYISGLLRKKVEVLAEACTDLLSHQKQLTVGLPPEPREKTISAPLPPEELTKLIYEASGQDISIAVLTQEIVVYLAMYVRAQPSLFVEMLRLRIGLIIQVMATELARSLNCSGEEASESLMNLSPFDMKNLLHHILSGKEFGVERSMRPIHSSTPSPAVSIHEVGHTGVTKTERSGINRLRSEMKQMTRRFSADEQLISVSQAASSSARSSKSARSSTPSSPTGTSSSDSGRQHMGWGERQGQWLRRRRLDGAINRVPVGFYQKVWKILQKCHGLSIDGYVLPSSTTQEMTPQEIKFAVHVESVLNRVPQPEYRQLLVEAIMVLTLLSDTEMNSIGGIIHVDQIVHMANQLFLQDQVSSGATDTLEKDQATGICHFFYDSAPSGAYGTMTYLTKAVASHLQELLPNSASGLLESEDRLEVGLSSAE

Target ORF information:

RefSeq Version	XM_010822157.2
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphorylase kinase, alpha 2 (liver) (PHKA2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_010822157.2

ORF Insert Sequence:

ATGAGGAGCA GGAGCAATTC CGGGGTCCGC TTAGACGGAT ACGCGCGGCT GGTGCAGCAA 
ACAATCCTCT GTTACCAGAA TCCCATCACG GGGCTGATGT CAGCCAGTCA TGAGCAGAAG 
GACGCCTGGG TACGGGATAA CATCTACAGC ATCCTGGCCG TGTGGGGCCT GGGCATGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAAT 
GTGGTGAAAC TGATGCGAGG TCTTCTCCAG TGCATGATGA GACAGGTGGA TAAAGTGGAG 
AGGTTCAAGC ACACTCAGAG TACCGAGGAC AGCCTGCACG CCAAGTACAA CACTGCCACC 
TGCAGCACTG TGGTGGGCGA CGACGAGTGG GGCCACCTCC AGGTGGATGC CACCTCCCTC 
TTCCTCCTGT TTCTGGCCCA GATGACAGCC TCCGGCTTAC GTATTATTTT CACTCTTGAC 
GAGGTGGCCT TCATACAGAA TCTTGTTTTT TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGAATGT GGGAACGTGG AGATAAGACT AATCAGGGTA TTCCAGAATT GAATGCAAGC 
TCTGTAGGAA TGGCCAAGGC AGCCCTTGAA GCAATTGATG AACTGGACCT TTTTGGAGCC 
CATGGAGGTC GCAAGTCTGT GATCCACGTC CTGCCGGATG AGGTGGAACA CTGCCAGTCT 
ATTCTGTTCT CCATGCTGCC AAGAGCCTCG ACGTCAAAAG AGATTGATGC TGGGCTTCTT 
TCCATTATTT CTTTCCCGGC CTTCGCGGTG GAAGATGTGA ACCTTGTGAA TGTGACAAAA 
AATGAAATTA TTTCCAAGCT CCAGGGGCGT TATGGATGCT GTCGCTTCCT TCGAGATGGT 
TATAAAACCC CAAGAGAGGA CCCAAACCGA TTGCATTATG ACCCTGCTGA ACTCAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTCATAAT AGATGGAGTC 
TTCAATGGTG ATGCCGTTCA GGTCCAAGAA TACCGTGAAG CCCTGGAGGG GATACTGATC 
AGAGGCAATA ACGGGGTCCA CTTGTTGCCT GAACTCTACG CCATCCCACC TGACAAGGTG 
GATGAAGAGT ATAAGAATCC TCACACGGTG GATAGAGTTC CCCTGGGGAA GGTGCCGCAT 
CTGTGGGGTC AGTCCTTGTA CATCCTCAGC TCCCTGTTGG CAGAGGGATT CCTTGCTGCT 
GGTGAAATTG ATCCCTTGAA CAGAAGATTT TCCACTTCAG TCAAACCTGA TGTCGTCGTA 
CAAGTGACTG TTCTGGCAGA AAACAACCAC ATTAAGGAGC TTCTGAGGAA ACATGGGGTC 
AACGTCCAGA GTATCGCTGA CATTCATCCA ATCCGAGTCC AGCCAGGCCG CATCCTTAGT 
CACATATATG CCAAGCTTGG ACGGAATAAG AATATGAGAT TGAGTGGGCG ACCGTATCGG 
CACATTGGTG TCCTTGGGAC ATCGAAACTA TACGTGATTA GGAACCAGAT CTTTACTTTC 
ACGCCCCAGT TCACCGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATCGTA 
GAGATGCTGA GGATTGAGCT GGCCTACCTG TGCACCTGCT GGCGGATGAC GGGCAGACCC 
ACGCTCACCT TCCCCATCAC GCATACCATG CTCACAAATG ATGGCTCAGA TATTCATTCC 
GCTGTTCTTG CTACAATTCG CAAACTAGAG GATGGATATT TTGGAGGAGC TAGAGTAAAA 
TTAGGGAGTC TTTCGGAATT TCTCACCACC TCTTTCTACA CGTATCTGAC CTTCCTGGAT 
CCAGACTGTG ATGAGAAGTT GTTTGATGAT GCCAGTGAAG GGACCTTCAG TCCTGATAGT 
GATTCAGACC TGGGAGGATA TTTGGAAGAC ACCTATAATC AAGAAAGCCA GGACGAGCTT 
GACCAGTATA TCAACCACCT TCTGCAAAGC ACATCCTCGA AGTGCTATCT GCCACCTCTC 
TGTAAGAAGA CAGAGGACAA CCAGGTTTTC AGTGCTATCC ATTCCACTCG GGATATACTT 
TCTGTGATGG CAAAAGCAAA GGGCTTGGAA ATTCCATTTG TTCCCATGGC TTTGCCAACT 
AAAGTTCTAA GTGCCCACCG TAAATCGCTG AATCTCGTTG ATTCTCCTCA GCCACTCCTA 
AAAAAGGCCC CTGAAGATGA CTTCCAGTTT CCCAGAGATG ACCATGGCGA CGTGGACTGT 
GAGAAGCTGG TTGAGCAGTT GAAAGACTGT TCGAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTGT ATGTAATAAA GGGTCCCAGC TGGGACACAA ATCTCTCTGG ACAGCACGGG 
GTCACTGTTC ACAACCTCCT CAGTGAGCTC TATGCGAAGG CCGGCTTGAA CCAGGAGTGG 
GGCCTGATCC GCTACATCTC CGGCCTCCTC CGGAAGAAAG TGGAGGTCCT GGCTGAGGCG 
TGCACGGACC TGCTGTCACA CCAGAAGCAG CTCACAGTGG GCCTGCCCCC AGAACCCCGG 
GAGAAGACCA TCTCTGCGCC TCTTCCCCCC GAGGAGCTCA CGAAGCTCAT CTACGAAGCC 
AGCGGGCAGG ACATCAGCAT CGCCGTGCTC ACACAGGAGA TCGTGGTGTA CCTGGCGATG 
TACGTCAGGG CACAGCCCAG CCTCTTCGTG GAGATGCTCC GGCTCCGAAT TGGACTGATC 
ATTCAGGTGA TGGCCACGGA GCTGGCACGG AGCCTGAACT GTTCAGGAGA AGAAGCTTCT 
GAAAGTCTGA TGAACCTCAG CCCTTTTGAT ATGAAAAATC TCCTGCACCA CATCTTAAGT 
GGCAAAGAAT TTGGTGTTGA AAGAAGCATG CGGCCCATTC ACTCCTCCAC ACCTAGCCCT 
GCTGTCTCCA TCCATGAGGT GGGCCACACT GGAGTCACCA AAACCGAGAG GAGCGGCATC 
AACAGGCTGA GGAGTGAGAT GAAACAGATG ACTAGGCGGT TTAGTGCTGA TGAACAGCTC 
ATTTCTGTGA GCCAGGCCGC ATCCAGCAGC GCGCGCTCCT CAAAGTCTGC GAGGTCCAGC 
ACCCCATCCT CACCCACGGG CACATCGTCA TCAGACTCGG GCAGGCAGCA CATGGGCTGG 
GGGGAACGGC AGGGCCAGTG GCTGCGCAGG AGAAGGCTGG ACGGCGCCAT CAACAGGGTC 
CCCGTGGGAT TCTACCAGAA GGTGTGGAAG ATCCTTCAGA AGTGCCATGG TCTGTCCATC 
GACGGTTACG TGCTCCCGTC CTCGACCACG CAAGAGATGA CCCCGCAGGA GATCAAGTTT 
GCTGTCCACG TTGAGTCAGT GCTGAACCGA GTGCCACAAC CCGAGTACCG GCAGCTGCTG 
GTGGAGGCCA TCATGGTGCT GACGCTGCTC TCGGACACAG AGATGAACAG CATCGGGGGC 
ATCATCCATG TGGACCAGAT CGTGCACATG GCCAACCAGC TGTTCCTACA AGACCAGGTG 
TCAAGTGGAG CCACGGACAC GCTGGAGAAA GACCAAGCCA CAGGCATCTG CCACTTCTTT 
TACGACAGCG CTCCAAGCGG GGCTTATGGG ACGATGACCT ACCTAACGAA GGCGGTGGCT 
TCTCATTTGC AGGAACTGCT GCCCAATTCA GCATCTGGTC TCCTGGAGTC TGAGGACAGA 
TTGGAAGTGG GTCTCTCAAG TGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA2 ( XM_005228390.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228447.1 Bos taurus phosphorylase kinase, alpha 2 (liver) (PHKA2), transcript variant X1, mRNA.

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CloneID	OBa35759
Clone ID Related Accession (Same CDS sequence)	XM_010822157.2 , XM_005228390.3
Accession Version	XM_005228390.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3750bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104772.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228390.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCA GGAGCAATTC CGGGGTCCGC TTAGACGGAT ACGCGCGGCT GGTGCAGCAA 
ACAATCCTCT GTTACCAGAA TCCCATCACG GGGCTGATGT CAGCCAGTCA TGAGCAGAAG 
GACGCCTGGG TACGGGATAA CATCTACAGC ATCCTGGCCG TGTGGGGCCT GGGCATGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAAT 
GTGGTGAAAC TGATGCGAGG TCTTCTCCAG TGCATGATGA GACAGGTGGA TAAAGTGGAG 
AGGTTCAAGC ACACTCAGAG TACCGAGGAC AGCCTGCACG CCAAGTACAA CACTGCCACC 
TGCAGCACTG TGGTGGGCGA CGACGAGTGG GGCCACCTCC AGGTGGATGC CACCTCCCTC 
TTCCTCCTGT TTCTGGCCCA GATGACAGCC TCCGGCTTAC GTATTATTTT CACTCTTGAC 
GAGGTGGCCT TCATACAGAA TCTTGTTTTT TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGAATGT GGGAACGTGG AGATAAGACT AATCAGGGTA TTCCAGAATT GAATGCAAGC 
TCTGTAGGAA TGGCCAAGGC AGCCCTTGAA GCAATTGATG AACTGGACCT TTTTGGAGCC 
CATGGAGGTC GCAAGTCTGT GATCCACGTC CTGCCGGATG AGGTGGAACA CTGCCAGTCT 
ATTCTGTTCT CCATGCTGCC AAGAGCCTCG ACGTCAAAAG AGATTGATGC TGGGCTTCTT 
TCCATTATTT CTTTCCCGGC CTTCGCGGTG GAAGATGTGA ACCTTGTGAA TGTGACAAAA 
AATGAAATTA TTTCCAAGCT CCAGGGGCGT TATGGATGCT GTCGCTTCCT TCGAGATGGT 
TATAAAACCC CAAGAGAGGA CCCAAACCGA TTGCATTATG ACCCTGCTGA ACTCAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTCATAAT AGATGGAGTC 
TTCAATGGTG ATGCCGTTCA GGTCCAAGAA TACCGTGAAG CCCTGGAGGG GATACTGATC 
AGAGGCAATA ACGGGGTCCA CTTGTTGCCT GAACTCTACG CCATCCCACC TGACAAGGTG 
GATGAAGAGT ATAAGAATCC TCACACGGTG GATAGAGTTC CCCTGGGGAA GGTGCCGCAT 
CTGTGGGGTC AGTCCTTGTA CATCCTCAGC TCCCTGTTGG CAGAGGGATT CCTTGCTGCT 
GGTGAAATTG ATCCCTTGAA CAGAAGATTT TCCACTTCAG TCAAACCTGA TGTCGTCGTA 
CAAGTGACTG TTCTGGCAGA AAACAACCAC ATTAAGGAGC TTCTGAGGAA ACATGGGGTC 
AACGTCCAGA GTATCGCTGA CATTCATCCA ATCCGAGTCC AGCCAGGCCG CATCCTTAGT 
CACATATATG CCAAGCTTGG ACGGAATAAG AATATGAGAT TGAGTGGGCG ACCGTATCGG 
CACATTGGTG TCCTTGGGAC ATCGAAACTA TACGTGATTA GGAACCAGAT CTTTACTTTC 
ACGCCCCAGT TCACCGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATCGTA 
GAGATGCTGA GGATTGAGCT GGCCTACCTG TGCACCTGCT GGCGGATGAC GGGCAGACCC 
ACGCTCACCT TCCCCATCAC GCATACCATG CTCACAAATG ATGGCTCAGA TATTCATTCC 
GCTGTTCTTG CTACAATTCG CAAACTAGAG GATGGATATT TTGGAGGAGC TAGAGTAAAA 
TTAGGGAGTC TTTCGGAATT TCTCACCACC TCTTTCTACA CGTATCTGAC CTTCCTGGAT 
CCAGACTGTG ATGAGAAGTT GTTTGATGAT GCCAGTGAAG GGACCTTCAG TCCTGATAGT 
GATTCAGACC TGGGAGGATA TTTGGAAGAC ACCTATAATC AAGAAAGCCA GGACGAGCTT 
GACCAGTATA TCAACCACCT TCTGCAAAGC ACATCCTCGA AGTGCTATCT GCCACCTCTC 
TGTAAGAAGA CAGAGGACAA CCAGGTTTTC AGTGCTATCC ATTCCACTCG GGATATACTT 
TCTGTGATGG CAAAAGCAAA GGGCTTGGAA ATTCCATTTG TTCCCATGGC TTTGCCAACT 
AAAGTTCTAA GTGCCCACCG TAAATCGCTG AATCTCGTTG ATTCTCCTCA GCCACTCCTA 
AAAAAGGCCC CTGAAGATGA CTTCCAGTTT CCCAGAGATG ACCATGGCGA CGTGGACTGT 
GAGAAGCTGG TTGAGCAGTT GAAAGACTGT TCGAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTGT ATGTAATAAA GGGTCCCAGC TGGGACACAA ATCTCTCTGG ACAGCACGGG 
GTCACTGTTC ACAACCTCCT CAGTGAGCTC TATGCGAAGG CCGGCTTGAA CCAGGAGTGG 
GGCCTGATCC GCTACATCTC CGGCCTCCTC CGGAAGAAAG TGGAGGTCCT GGCTGAGGCG 
TGCACGGACC TGCTGTCACA CCAGAAGCAG CTCACAGTGG GCCTGCCCCC AGAACCCCGG 
GAGAAGACCA TCTCTGCGCC TCTTCCCCCC GAGGAGCTCA CGAAGCTCAT CTACGAAGCC 
AGCGGGCAGG ACATCAGCAT CGCCGTGCTC ACACAGGAGA TCGTGGTGTA CCTGGCGATG 
TACGTCAGGG CACAGCCCAG CCTCTTCGTG GAGATGCTCC GGCTCCGAAT TGGACTGATC 
ATTCAGGTGA TGGCCACGGA GCTGGCACGG AGCCTGAACT GTTCAGGAGA AGAAGCTTCT 
GAAAGTCTGA TGAACCTCAG CCCTTTTGAT ATGAAAAATC TCCTGCACCA CATCTTAAGT 
GGCAAAGAAT TTGGTGTTGA AAGAAGCATG CGGCCCATTC ACTCCTCCAC ACCTAGCCCT 
GCTGTCTCCA TCCATGAGGT GGGCCACACT GGAGTCACCA AAACCGAGAG GAGCGGCATC 
AACAGGCTGA GGAGTGAGAT GAAACAGATG ACTAGGCGGT TTAGTGCTGA TGAACAGCTC 
ATTTCTGTGA GCCAGGCCGC ATCCAGCAGC GCGCGCTCCT CAAAGTCTGC GAGGTCCAGC 
ACCCCATCCT CACCCACGGG CACATCGTCA TCAGACTCGG GCAGGCAGCA CATGGGCTGG 
GGGGAACGGC AGGGCCAGTG GCTGCGCAGG AGAAGGCTGG ACGGCGCCAT CAACAGGGTC 
CCCGTGGGAT TCTACCAGAA GGTGTGGAAG ATCCTTCAGA AGTGCCATGG TCTGTCCATC 
GACGGTTACG TGCTCCCGTC CTCGACCACG CAAGAGATGA CCCCGCAGGA GATCAAGTTT 
GCTGTCCACG TTGAGTCAGT GCTGAACCGA GTGCCACAAC CCGAGTACCG GCAGCTGCTG 
GTGGAGGCCA TCATGGTGCT GACGCTGCTC TCGGACACAG AGATGAACAG CATCGGGGGC 
ATCATCCATG TGGACCAGAT CGTGCACATG GCCAACCAGC TGTTCCTACA AGACCAGGTG 
TCAAGTGGAG CCACGGACAC GCTGGAGAAA GACCAAGCCA CAGGCATCTG CCACTTCTTT 
TACGACAGCG CTCCAAGCGG GGCTTATGGG ACGATGACCT ACCTAACGAA GGCGGTGGCT 
TCTCATTTGC AGGAACTGCT GCCCAATTCA GCATCTGGTC TCCTGGAGTC TGAGGACAGA 
TTGGAAGTGG GTCTCTCAAG TGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228447.1
CDS	251..4000
Translation	MRSRSNSGVRLDGYARLVQQTILCYQNPITGLMSASHEQKDAWVRDNIYSILAVWGLGMAYRKNADRDEDKAKAYELEQNVVKLMRGLLQCMMRQVDKVERFKHTQSTEDSLHAKYNTATCSTVVGDDEWGHLQVDATSLFLLFLAQMTASGLRIIFTLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGMAKAALEAIDELDLFGAHGGRKSVIHVLPDEVEHCQSILFSMLPRASTSKEIDAGLLSIISFPAFAVEDVNLVNVTKNEIISKLQGRYGCCRFLRDGYKTPREDPNRLHYDPAELKLFENIECEWPVFWTYFIIDGVFNGDAVQVQEYREALEGILIRGNNGVHLLPELYAIPPDKVDEEYKNPHTVDRVPLGKVPHLWGQSLYILSSLLAEGFLAAGEIDPLNRRFSTSVKPDVVVQVTVLAENNHIKELLRKHGVNVQSIADIHPIRVQPGRILSHIYAKLGRNKNMRLSGRPYRHIGVLGTSKLYVIRNQIFTFTPQFTDQHHFYLALDNEMIVEMLRIELAYLCTCWRMTGRPTLTFPITHTMLTNDGSDIHSAVLATIRKLEDGYFGGARVKLGSLSEFLTTSFYTYLTFLDPDCDEKLFDDASEGTFSPDSDSDLGGYLEDTYNQESQDELDQYINHLLQSTSSKCYLPPLCKKTEDNQVFSAIHSTRDILSVMAKAKGLEIPFVPMALPTKVLSAHRKSLNLVDSPQPLLKKAPEDDFQFPRDDHGDVDCEKLVEQLKDCSNLQDQADILYILYVIKGPSWDTNLSGQHGVTVHNLLSELYAKAGLNQEWGLIRYISGLLRKKVEVLAEACTDLLSHQKQLTVGLPPEPREKTISAPLPPEELTKLIYEASGQDISIAVLTQEIVVYLAMYVRAQPSLFVEMLRLRIGLIIQVMATELARSLNCSGEEASESLMNLSPFDMKNLLHHILSGKEFGVERSMRPIHSSTPSPAVSIHEVGHTGVTKTERSGINRLRSEMKQMTRRFSADEQLISVSQAASSSARSSKSARSSTPSSPTGTSSSDSGRQHMGWGERQGQWLRRRRLDGAINRVPVGFYQKVWKILQKCHGLSIDGYVLPSSTTQEMTPQEIKFAVHVESVLNRVPQPEYRQLLVEAIMVLTLLSDTEMNSIGGIIHVDQIVHMANQLFLQDQVSSGATDTLEKDQATGICHFFYDSAPSGAYGTMTYLTKAVASHLQELLPNSASGLLESEDRLEVGLSSAE

Target ORF information:

RefSeq Version	XM_005228390.3
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphorylase kinase, alpha 2 (liver) (PHKA2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228390.3

ORF Insert Sequence:

ATGAGGAGCA GGAGCAATTC CGGGGTCCGC TTAGACGGAT ACGCGCGGCT GGTGCAGCAA 
ACAATCCTCT GTTACCAGAA TCCCATCACG GGGCTGATGT CAGCCAGTCA TGAGCAGAAG 
GACGCCTGGG TACGGGATAA CATCTACAGC ATCCTGGCCG TGTGGGGCCT GGGCATGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAAT 
GTGGTGAAAC TGATGCGAGG TCTTCTCCAG TGCATGATGA GACAGGTGGA TAAAGTGGAG 
AGGTTCAAGC ACACTCAGAG TACCGAGGAC AGCCTGCACG CCAAGTACAA CACTGCCACC 
TGCAGCACTG TGGTGGGCGA CGACGAGTGG GGCCACCTCC AGGTGGATGC CACCTCCCTC 
TTCCTCCTGT TTCTGGCCCA GATGACAGCC TCCGGCTTAC GTATTATTTT CACTCTTGAC 
GAGGTGGCCT TCATACAGAA TCTTGTTTTT TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGAATGT GGGAACGTGG AGATAAGACT AATCAGGGTA TTCCAGAATT GAATGCAAGC 
TCTGTAGGAA TGGCCAAGGC AGCCCTTGAA GCAATTGATG AACTGGACCT TTTTGGAGCC 
CATGGAGGTC GCAAGTCTGT GATCCACGTC CTGCCGGATG AGGTGGAACA CTGCCAGTCT 
ATTCTGTTCT CCATGCTGCC AAGAGCCTCG ACGTCAAAAG AGATTGATGC TGGGCTTCTT 
TCCATTATTT CTTTCCCGGC CTTCGCGGTG GAAGATGTGA ACCTTGTGAA TGTGACAAAA 
AATGAAATTA TTTCCAAGCT CCAGGGGCGT TATGGATGCT GTCGCTTCCT TCGAGATGGT 
TATAAAACCC CAAGAGAGGA CCCAAACCGA TTGCATTATG ACCCTGCTGA ACTCAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTCATAAT AGATGGAGTC 
TTCAATGGTG ATGCCGTTCA GGTCCAAGAA TACCGTGAAG CCCTGGAGGG GATACTGATC 
AGAGGCAATA ACGGGGTCCA CTTGTTGCCT GAACTCTACG CCATCCCACC TGACAAGGTG 
GATGAAGAGT ATAAGAATCC TCACACGGTG GATAGAGTTC CCCTGGGGAA GGTGCCGCAT 
CTGTGGGGTC AGTCCTTGTA CATCCTCAGC TCCCTGTTGG CAGAGGGATT CCTTGCTGCT 
GGTGAAATTG ATCCCTTGAA CAGAAGATTT TCCACTTCAG TCAAACCTGA TGTCGTCGTA 
CAAGTGACTG TTCTGGCAGA AAACAACCAC ATTAAGGAGC TTCTGAGGAA ACATGGGGTC 
AACGTCCAGA GTATCGCTGA CATTCATCCA ATCCGAGTCC AGCCAGGCCG CATCCTTAGT 
CACATATATG CCAAGCTTGG ACGGAATAAG AATATGAGAT TGAGTGGGCG ACCGTATCGG 
CACATTGGTG TCCTTGGGAC ATCGAAACTA TACGTGATTA GGAACCAGAT CTTTACTTTC 
ACGCCCCAGT TCACCGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATCGTA 
GAGATGCTGA GGATTGAGCT GGCCTACCTG TGCACCTGCT GGCGGATGAC GGGCAGACCC 
ACGCTCACCT TCCCCATCAC GCATACCATG CTCACAAATG ATGGCTCAGA TATTCATTCC 
GCTGTTCTTG CTACAATTCG CAAACTAGAG GATGGATATT TTGGAGGAGC TAGAGTAAAA 
TTAGGGAGTC TTTCGGAATT TCTCACCACC TCTTTCTACA CGTATCTGAC CTTCCTGGAT 
CCAGACTGTG ATGAGAAGTT GTTTGATGAT GCCAGTGAAG GGACCTTCAG TCCTGATAGT 
GATTCAGACC TGGGAGGATA TTTGGAAGAC ACCTATAATC AAGAAAGCCA GGACGAGCTT 
GACCAGTATA TCAACCACCT TCTGCAAAGC ACATCCTCGA AGTGCTATCT GCCACCTCTC 
TGTAAGAAGA CAGAGGACAA CCAGGTTTTC AGTGCTATCC ATTCCACTCG GGATATACTT 
TCTGTGATGG CAAAAGCAAA GGGCTTGGAA ATTCCATTTG TTCCCATGGC TTTGCCAACT 
AAAGTTCTAA GTGCCCACCG TAAATCGCTG AATCTCGTTG ATTCTCCTCA GCCACTCCTA 
AAAAAGGCCC CTGAAGATGA CTTCCAGTTT CCCAGAGATG ACCATGGCGA CGTGGACTGT 
GAGAAGCTGG TTGAGCAGTT GAAAGACTGT TCGAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTGT ATGTAATAAA GGGTCCCAGC TGGGACACAA ATCTCTCTGG ACAGCACGGG 
GTCACTGTTC ACAACCTCCT CAGTGAGCTC TATGCGAAGG CCGGCTTGAA CCAGGAGTGG 
GGCCTGATCC GCTACATCTC CGGCCTCCTC CGGAAGAAAG TGGAGGTCCT GGCTGAGGCG 
TGCACGGACC TGCTGTCACA CCAGAAGCAG CTCACAGTGG GCCTGCCCCC AGAACCCCGG 
GAGAAGACCA TCTCTGCGCC TCTTCCCCCC GAGGAGCTCA CGAAGCTCAT CTACGAAGCC 
AGCGGGCAGG ACATCAGCAT CGCCGTGCTC ACACAGGAGA TCGTGGTGTA CCTGGCGATG 
TACGTCAGGG CACAGCCCAG CCTCTTCGTG GAGATGCTCC GGCTCCGAAT TGGACTGATC 
ATTCAGGTGA TGGCCACGGA GCTGGCACGG AGCCTGAACT GTTCAGGAGA AGAAGCTTCT 
GAAAGTCTGA TGAACCTCAG CCCTTTTGAT ATGAAAAATC TCCTGCACCA CATCTTAAGT 
GGCAAAGAAT TTGGTGTTGA AAGAAGCATG CGGCCCATTC ACTCCTCCAC ACCTAGCCCT 
GCTGTCTCCA TCCATGAGGT GGGCCACACT GGAGTCACCA AAACCGAGAG GAGCGGCATC 
AACAGGCTGA GGAGTGAGAT GAAACAGATG ACTAGGCGGT TTAGTGCTGA TGAACAGCTC 
ATTTCTGTGA GCCAGGCCGC ATCCAGCAGC GCGCGCTCCT CAAAGTCTGC GAGGTCCAGC 
ACCCCATCCT CACCCACGGG CACATCGTCA TCAGACTCGG GCAGGCAGCA CATGGGCTGG 
GGGGAACGGC AGGGCCAGTG GCTGCGCAGG AGAAGGCTGG ACGGCGCCAT CAACAGGGTC 
CCCGTGGGAT TCTACCAGAA GGTGTGGAAG ATCCTTCAGA AGTGCCATGG TCTGTCCATC 
GACGGTTACG TGCTCCCGTC CTCGACCACG CAAGAGATGA CCCCGCAGGA GATCAAGTTT 
GCTGTCCACG TTGAGTCAGT GCTGAACCGA GTGCCACAAC CCGAGTACCG GCAGCTGCTG 
GTGGAGGCCA TCATGGTGCT GACGCTGCTC TCGGACACAG AGATGAACAG CATCGGGGGC 
ATCATCCATG TGGACCAGAT CGTGCACATG GCCAACCAGC TGTTCCTACA AGACCAGGTG 
TCAAGTGGAG CCACGGACAC GCTGGAGAAA GACCAAGCCA CAGGCATCTG CCACTTCTTT 
TACGACAGCG CTCCAAGCGG GGCTTATGGG ACGATGACCT ACCTAACGAA GGCGGTGGCT 
TCTCATTTGC AGGAACTGCT GCCCAATTCA GCATCTGGTC TCCTGGAGTC TGAGGACAGA 
TTGGAAGTGG GTCTCTCAAG TGCAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA2 ( XM_005228391.2 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228448.1 Bos taurus phosphorylase kinase, alpha 2 (liver) (PHKA2), transcript variant X3, mRNA.

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CloneID	OBa35760
Clone ID Related Accession (Same CDS sequence)	NM_001191545.1 , XM_005228391.2 , XM_024987966.1
Accession Version	XM_005228391.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104772.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228391.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCA GGAGCAATTC CGGGGTCCGC TTAGACGGAT ACGCGCGGCT GGTGCAGCAA 
ACAATCCTCT GTTACCAGAA TCCCATCACG GGGCTGATGT CAGCCAGTCA TGAGCAGAAG 
GACGCCTGGG TACGGGATAA CATCTACAGC ATCCTGGCCG TGTGGGGCCT GGGCATGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAAT 
GTGGTGAAAC TGATGCGAGG TCTTCTCCAG TGCATGATGA GACAGGTGGA TAAAGTGGAG 
AGGTTCAAGC ACACTCAGAG TACCGAGGAC AGCCTGCACG CCAAGTACAA CACTGCCACC 
TGCAGCACTG TGGTGGGCGA CGACGAGTGG GGCCACCTCC AGGTGGATGC CACCTCCCTC 
TTCCTCCTGT TTCTGGCCCA GATGACAGCC TCCGGCTTAC GTATTATTTT CACTCTTGAC 
GAGGTGGCCT TCATACAGAA TCTTGTTTTT TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGAATGT GGGAACGTGG AGATAAGACT AATCAGGGTA TTCCAGAATT GAATGCAAGC 
TCTGTAGGAA TGGCCAAGGC AGCCCTTGAA GCAATTGATG AACTGGACCT TTTTGGAGCC 
CATGGAGGTC GCAAGTCTGT GATCCACGTC CTGCCGGATG AGGTGGAACA CTGCCAGTCT 
ATTCTGTTCT CCATGCTGCC AAGAGCCTCG ACGTCAAAAG AGATTGATGC TGGGCTTCTT 
TCCATTATTT CTTTCCCGGC CTTCGCGGTG GAAGATGTGA ACCTTGTGAA TGTGACAAAA 
AATGAAATTA TTTCCAAGCT CCAGGGGCGT TATGGATGCT GTCGCTTCCT TCGAGATGGT 
TATAAAACCC CAAGAGAGGA CCCAAACCGA TTGCATTATG ACCCTGCTGA ACTCAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTCATAAT AGATGGAGTC 
TTCAATGGTG ATGCCGTTCA GGTCCAAGAA TACCGTGAAG CCCTGGAGGG GATACTGATC 
AGAGGCAATA ACGGGGTCCA CTTGTTGCCT GAACTCTACG CCATCCCACC TGACAAGGTG 
GATGAAGAGT ATAAGAATCC TCACACGGTG GATAGAGTTC CCCTGGGGAA GGTGCCGCAT 
CTGTGGGGTC AGTCCTTGTA CATCCTCAGC TCCCTGTTGG CAGAGGGATT CCTTGCTGCT 
GGTGAAATTG ATCCCTTGAA CAGAAGATTT TCCACTTCAG TCAAACCTGA TGTCGTCGTA 
CAAGTGACTG TTCTGGCAGA AAACAACCAC ATTAAGGAGC TTCTGAGGAA ACATGGGGTC 
AACGTCCAGA GTATCGCTGA CATTCATCCA ATCCGAGTCC AGCCAGGCCG CATCCTTAGT 
CACATATATG CCAAGCTTGG ACGGAATAAG AATATGAGAT TGAGTGGGCG ACCGTATCGG 
CACATTGGTG TCCTTGGGAC ATCGAAACTA TACGTGATTA GGAACCAGAT CTTTACTTTC 
ACGCCCCAGT TCACCGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATCGTA 
GAGATGCTGA GGATTGAGCT GGCCTACCTG TGCACCTGCT GGCGGATGAC GGGCAGACCC 
ACGCTCACCT TCCCCATCAC GCATACCATG CTCACAAATG ATGGCTCAGA TATTCATTCC 
GCTGTTCTTG CTACAATTCG CAAACTAGAG GATGGATATT TTGGAGGAGC TAGAGTAAAA 
TTAGGGAGTC TTTCGGAATT TCTCACCACC TCTTTCTACA CGTATCTGAC CTTCCTGGAT 
CCAGACTGTG ATGAGAAGTT GTTTGATGAT GCCAGTGAAG GGACCTTCAG TCCTGATAGT 
GATTCAGACC TGGGAGGATA TTTGGAAGAC ACCTATAATC AAGAAAGCCA GGACGAGCTT 
GACCAGTATA TCAACCACCT TCTGCAAAGC ACATCCTCGA AGTGCTATCT GCCACCTCTC 
TGTAAGAAGA CAGAGGACAA CCAGGTTTTC AGTGCTATCC ATTCCACTCG GGATATACTT 
TCTGTGATGG CAAAAGCAAA GGGCTTGGAA ATTCCATTTG TTCCCATGGC TTTGCCAACT 
AAAGTTCTAA GTGCCCACCG TAAATCGCTG AATCTCGTTG ATTCTCCTCA GCCACTCCTA 
AAAAAGGCCC CTGAAGATGA CTTCCAGTTT CCCAGAGATG ACCATGGCGA CGTGGACTGT 
GAGAAGCTGG TTGAGCAGTT GAAAGACTGT TCGAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTGT ATGTAATAAA GGGTCCCAGC TGGGACACAA ATCTCTCTGG ACAGCACGGG 
GTCACTGTTC ACAACCTCCT CAGTGAGCTC TATGCGAAGG CCGGCTTGAA CCAGGAGTGG 
GGCCTGATCC GCTACATCTC CGGCCTCCTC CGGAAGAAAG TGGAGGTCCT GGCTGAGGCG 
TGCACGGACC TGCTGTCACA CCAGAAGCAG CTCACAGTGG GCCTGCCCCC AGAACCCCGG 
GAGAAGACCA TCTCTGCGCC TCTTCCCCCC GAGGAGCTCA CGAAGCTCAT CTACGAAGCC 
AGCGGGCAGG ACATCAGCAT CGCCGTGCTC ACACAGGAGA TCGTGGTGTA CCTGGCGATG 
TACGTCAGGG CACAGCCCAG CCTCTTCGTG GAGATGCTCC GGCTCCGAAT TGGACTGATC 
ATTCAGGTGA TGGCCACGGA GCTGGCACGG AGCCTGAACT GTTCAGGAGA AGAAGCTTCT 
GAAAGTCTGA TGAACCTCAG CCCTTTTGAT ATGAAAAATC TCCTGCACCA CATCTTAAGT 
GGCAAAGAAT TTGGTGTTGA AAGAAGCATG CGGCCCATTC ACTCCTCCAC ACCTAGCCCT 
GCTGTCTCCA TCCATGAGGT GGGCCACACT GGAGTCACCA AAACCGAGAG GAGCGGCATC 
AACAGGCTGA GGAGTGAGAT GAAACAGATG ACTAGGCGGT TTAGTGCTGA TGAACAGCTC 
ATTTCTGTGA GCCAGGCCGC ATCCAGCAGC GCGCGCTCCT CAAAGTCTGC GAGGTCCAGC 
ACCCCATCCT CACCCACGGG CACATCGTCA TCAGACTCGG GCAGGCAGCA CATGGGCTGG 
GGGGAACGGC AGGGCCAGTG GCTGCGCAGG AGAAGGCTGG ACGGCGCCAT CAACAGGGTC 
CCCGTGGGAT TCTACCAGAA GGTGTGGAAG ATCCTTCAGA AGTGCCATGG TCTGTCCATC 
GACGGTTACG TGCTCCCGTC CTCGACCACG CAAGAGATGA CCCCGCAGGA GATCAAGTTT 
GCTGTCCACG TTGAGTCAGT GCTGAACCGA GTGCCACAAC CCGAGTACCG GCAGCTGCTG 
GTGGAGGCCA TCATGGTGCT GACGCTGCTC TCGGACACAG AGATGAACAG CATCGGGGGC 
ATCATCCATG TGGACCAGAT CGTGCACATG GCCAACCAGC TGTTCCTACA AGACCAGGTG 
TCAAGTGGAG CCACGGACAC GCTGGAGAAA GACCAAGCCA CAGGCATCTG CCACTTCTTT 
TACGACAGCG CTCCAAGCGG GGCTTATGGG ACGATGACCT ACCTAACGAA GGCGGTGGCT 
TCTCATTTGC AGGAACTGCT GCCCAATTCA GGTTGCCAGA TGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228448.1
CDS	250..3957
Translation	MRSRSNSGVRLDGYARLVQQTILCYQNPITGLMSASHEQKDAWVRDNIYSILAVWGLGMAYRKNADRDEDKAKAYELEQNVVKLMRGLLQCMMRQVDKVERFKHTQSTEDSLHAKYNTATCSTVVGDDEWGHLQVDATSLFLLFLAQMTASGLRIIFTLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGMAKAALEAIDELDLFGAHGGRKSVIHVLPDEVEHCQSILFSMLPRASTSKEIDAGLLSIISFPAFAVEDVNLVNVTKNEIISKLQGRYGCCRFLRDGYKTPREDPNRLHYDPAELKLFENIECEWPVFWTYFIIDGVFNGDAVQVQEYREALEGILIRGNNGVHLLPELYAIPPDKVDEEYKNPHTVDRVPLGKVPHLWGQSLYILSSLLAEGFLAAGEIDPLNRRFSTSVKPDVVVQVTVLAENNHIKELLRKHGVNVQSIADIHPIRVQPGRILSHIYAKLGRNKNMRLSGRPYRHIGVLGTSKLYVIRNQIFTFTPQFTDQHHFYLALDNEMIVEMLRIELAYLCTCWRMTGRPTLTFPITHTMLTNDGSDIHSAVLATIRKLEDGYFGGARVKLGSLSEFLTTSFYTYLTFLDPDCDEKLFDDASEGTFSPDSDSDLGGYLEDTYNQESQDELDQYINHLLQSTSSKCYLPPLCKKTEDNQVFSAIHSTRDILSVMAKAKGLEIPFVPMALPTKVLSAHRKSLNLVDSPQPLLKKAPEDDFQFPRDDHGDVDCEKLVEQLKDCSNLQDQADILYILYVIKGPSWDTNLSGQHGVTVHNLLSELYAKAGLNQEWGLIRYISGLLRKKVEVLAEACTDLLSHQKQLTVGLPPEPREKTISAPLPPEELTKLIYEASGQDISIAVLTQEIVVYLAMYVRAQPSLFVEMLRLRIGLIIQVMATELARSLNCSGEEASESLMNLSPFDMKNLLHHILSGKEFGVERSMRPIHSSTPSPAVSIHEVGHTGVTKTERSGINRLRSEMKQMTRRFSADEQLISVSQAASSSARSSKSARSSTPSSPTGTSSSDSGRQHMGWGERQGQWLRRRRLDGAINRVPVGFYQKVWKILQKCHGLSIDGYVLPSSTTQEMTPQEIKFAVHVESVLNRVPQPEYRQLLVEAIMVLTLLSDTEMNSIGGIIHVDQIVHMANQLFLQDQVSSGATDTLEKDQATGICHFFYDSAPSGAYGTMTYLTKAVASHLQELLPNSGCQMQ

Target ORF information:

RefSeq Version	XM_005228391.2
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphorylase kinase, alpha 2 (liver) (PHKA2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228391.2

ORF Insert Sequence:

ATGAGGAGCA GGAGCAATTC CGGGGTCCGC TTAGACGGAT ACGCGCGGCT GGTGCAGCAA 
ACAATCCTCT GTTACCAGAA TCCCATCACG GGGCTGATGT CAGCCAGTCA TGAGCAGAAG 
GACGCCTGGG TACGGGATAA CATCTACAGC ATCCTGGCCG TGTGGGGCCT GGGCATGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAAT 
GTGGTGAAAC TGATGCGAGG TCTTCTCCAG TGCATGATGA GACAGGTGGA TAAAGTGGAG 
AGGTTCAAGC ACACTCAGAG TACCGAGGAC AGCCTGCACG CCAAGTACAA CACTGCCACC 
TGCAGCACTG TGGTGGGCGA CGACGAGTGG GGCCACCTCC AGGTGGATGC CACCTCCCTC 
TTCCTCCTGT TTCTGGCCCA GATGACAGCC TCCGGCTTAC GTATTATTTT CACTCTTGAC 
GAGGTGGCCT TCATACAGAA TCTTGTTTTT TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGAATGT GGGAACGTGG AGATAAGACT AATCAGGGTA TTCCAGAATT GAATGCAAGC 
TCTGTAGGAA TGGCCAAGGC AGCCCTTGAA GCAATTGATG AACTGGACCT TTTTGGAGCC 
CATGGAGGTC GCAAGTCTGT GATCCACGTC CTGCCGGATG AGGTGGAACA CTGCCAGTCT 
ATTCTGTTCT CCATGCTGCC AAGAGCCTCG ACGTCAAAAG AGATTGATGC TGGGCTTCTT 
TCCATTATTT CTTTCCCGGC CTTCGCGGTG GAAGATGTGA ACCTTGTGAA TGTGACAAAA 
AATGAAATTA TTTCCAAGCT CCAGGGGCGT TATGGATGCT GTCGCTTCCT TCGAGATGGT 
TATAAAACCC CAAGAGAGGA CCCAAACCGA TTGCATTATG ACCCTGCTGA ACTCAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTCATAAT AGATGGAGTC 
TTCAATGGTG ATGCCGTTCA GGTCCAAGAA TACCGTGAAG CCCTGGAGGG GATACTGATC 
AGAGGCAATA ACGGGGTCCA CTTGTTGCCT GAACTCTACG CCATCCCACC TGACAAGGTG 
GATGAAGAGT ATAAGAATCC TCACACGGTG GATAGAGTTC CCCTGGGGAA GGTGCCGCAT 
CTGTGGGGTC AGTCCTTGTA CATCCTCAGC TCCCTGTTGG CAGAGGGATT CCTTGCTGCT 
GGTGAAATTG ATCCCTTGAA CAGAAGATTT TCCACTTCAG TCAAACCTGA TGTCGTCGTA 
CAAGTGACTG TTCTGGCAGA AAACAACCAC ATTAAGGAGC TTCTGAGGAA ACATGGGGTC 
AACGTCCAGA GTATCGCTGA CATTCATCCA ATCCGAGTCC AGCCAGGCCG CATCCTTAGT 
CACATATATG CCAAGCTTGG ACGGAATAAG AATATGAGAT TGAGTGGGCG ACCGTATCGG 
CACATTGGTG TCCTTGGGAC ATCGAAACTA TACGTGATTA GGAACCAGAT CTTTACTTTC 
ACGCCCCAGT TCACCGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATCGTA 
GAGATGCTGA GGATTGAGCT GGCCTACCTG TGCACCTGCT GGCGGATGAC GGGCAGACCC 
ACGCTCACCT TCCCCATCAC GCATACCATG CTCACAAATG ATGGCTCAGA TATTCATTCC 
GCTGTTCTTG CTACAATTCG CAAACTAGAG GATGGATATT TTGGAGGAGC TAGAGTAAAA 
TTAGGGAGTC TTTCGGAATT TCTCACCACC TCTTTCTACA CGTATCTGAC CTTCCTGGAT 
CCAGACTGTG ATGAGAAGTT GTTTGATGAT GCCAGTGAAG GGACCTTCAG TCCTGATAGT 
GATTCAGACC TGGGAGGATA TTTGGAAGAC ACCTATAATC AAGAAAGCCA GGACGAGCTT 
GACCAGTATA TCAACCACCT TCTGCAAAGC ACATCCTCGA AGTGCTATCT GCCACCTCTC 
TGTAAGAAGA CAGAGGACAA CCAGGTTTTC AGTGCTATCC ATTCCACTCG GGATATACTT 
TCTGTGATGG CAAAAGCAAA GGGCTTGGAA ATTCCATTTG TTCCCATGGC TTTGCCAACT 
AAAGTTCTAA GTGCCCACCG TAAATCGCTG AATCTCGTTG ATTCTCCTCA GCCACTCCTA 
AAAAAGGCCC CTGAAGATGA CTTCCAGTTT CCCAGAGATG ACCATGGCGA CGTGGACTGT 
GAGAAGCTGG TTGAGCAGTT GAAAGACTGT TCGAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTGT ATGTAATAAA GGGTCCCAGC TGGGACACAA ATCTCTCTGG ACAGCACGGG 
GTCACTGTTC ACAACCTCCT CAGTGAGCTC TATGCGAAGG CCGGCTTGAA CCAGGAGTGG 
GGCCTGATCC GCTACATCTC CGGCCTCCTC CGGAAGAAAG TGGAGGTCCT GGCTGAGGCG 
TGCACGGACC TGCTGTCACA CCAGAAGCAG CTCACAGTGG GCCTGCCCCC AGAACCCCGG 
GAGAAGACCA TCTCTGCGCC TCTTCCCCCC GAGGAGCTCA CGAAGCTCAT CTACGAAGCC 
AGCGGGCAGG ACATCAGCAT CGCCGTGCTC ACACAGGAGA TCGTGGTGTA CCTGGCGATG 
TACGTCAGGG CACAGCCCAG CCTCTTCGTG GAGATGCTCC GGCTCCGAAT TGGACTGATC 
ATTCAGGTGA TGGCCACGGA GCTGGCACGG AGCCTGAACT GTTCAGGAGA AGAAGCTTCT 
GAAAGTCTGA TGAACCTCAG CCCTTTTGAT ATGAAAAATC TCCTGCACCA CATCTTAAGT 
GGCAAAGAAT TTGGTGTTGA AAGAAGCATG CGGCCCATTC ACTCCTCCAC ACCTAGCCCT 
GCTGTCTCCA TCCATGAGGT GGGCCACACT GGAGTCACCA AAACCGAGAG GAGCGGCATC 
AACAGGCTGA GGAGTGAGAT GAAACAGATG ACTAGGCGGT TTAGTGCTGA TGAACAGCTC 
ATTTCTGTGA GCCAGGCCGC ATCCAGCAGC GCGCGCTCCT CAAAGTCTGC GAGGTCCAGC 
ACCCCATCCT CACCCACGGG CACATCGTCA TCAGACTCGG GCAGGCAGCA CATGGGCTGG 
GGGGAACGGC AGGGCCAGTG GCTGCGCAGG AGAAGGCTGG ACGGCGCCAT CAACAGGGTC 
CCCGTGGGAT TCTACCAGAA GGTGTGGAAG ATCCTTCAGA AGTGCCATGG TCTGTCCATC 
GACGGTTACG TGCTCCCGTC CTCGACCACG CAAGAGATGA CCCCGCAGGA GATCAAGTTT 
GCTGTCCACG TTGAGTCAGT GCTGAACCGA GTGCCACAAC CCGAGTACCG GCAGCTGCTG 
GTGGAGGCCA TCATGGTGCT GACGCTGCTC TCGGACACAG AGATGAACAG CATCGGGGGC 
ATCATCCATG TGGACCAGAT CGTGCACATG GCCAACCAGC TGTTCCTACA AGACCAGGTG 
TCAAGTGGAG CCACGGACAC GCTGGAGAAA GACCAAGCCA CAGGCATCTG CCACTTCTTT 
TACGACAGCG CTCCAAGCGG GGCTTATGGG ACGATGACCT ACCTAACGAA GGCGGTGGCT 
TCTCATTTGC AGGAACTGCT GCCCAATTCA GGTTGCCAGA TGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA2 ( XM_024987966.1 ) cDNA ORF clone, Bos taurus(cattle) -> XP_024843734.1 Bos taurus phosphorylase kinase regulatory subunit alpha 2 (PHKA2), transcript variant X1, mRNA.

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CloneID	OBa35760
Clone ID Related Accession (Same CDS sequence)	NM_001191545.1 , XM_005228391.2 , XM_024987966.1
Accession Version	XM_024987966.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3708bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-05-06
Organism	Bos taurus(cattle)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_037357.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Bos taurus Annotation Release 106 Annotation Version :: 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.0 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCA GGAGCAATTC CGGGGTCCGC TTAGACGGAT ACGCGCGGCT GGTGCAGCAA 
ACAATCCTCT GTTACCAGAA TCCCATCACG GGGCTGATGT CAGCCAGTCA TGAGCAGAAG 
GACGCCTGGG TACGGGATAA CATCTACAGC ATCCTGGCCG TGTGGGGCCT GGGCATGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAAT 
GTGGTGAAAC TGATGCGAGG TCTTCTCCAG TGCATGATGA GACAGGTGGA TAAAGTGGAG 
AGGTTCAAGC ACACTCAGAG TACCGAGGAC AGCCTGCACG CCAAGTACAA CACTGCCACC 
TGCAGCACTG TGGTGGGCGA CGACGAGTGG GGCCACCTCC AGGTGGATGC CACCTCCCTC 
TTCCTCCTGT TTCTGGCCCA GATGACAGCC TCCGGCTTAC GTATTATTTT CACTCTTGAC 
GAGGTGGCCT TCATACAGAA TCTTGTTTTT TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGAATGT GGGAACGTGG AGATAAGACT AATCAGGGTA TTCCAGAATT GAATGCAAGC 
TCTGTAGGAA TGGCCAAGGC AGCCCTTGAA GCAATTGATG AACTGGACCT TTTTGGAGCC 
CATGGAGGTC GCAAGTCTGT GATCCACGTC CTGCCGGATG AGGTGGAACA CTGCCAGTCT 
ATTCTGTTCT CCATGCTGCC AAGAGCCTCG ACGTCAAAAG AGATTGATGC TGGGCTTCTT 
TCCATTATTT CTTTCCCGGC CTTCGCGGTG GAAGATGTGA ACCTTGTGAA TGTGACAAAA 
AATGAAATTA TTTCCAAGCT CCAGGGGCGT TATGGATGCT GTCGCTTCCT TCGAGATGGT 
TATAAAACCC CAAGAGAGGA CCCAAACCGA TTGCATTATG ACCCTGCTGA ACTCAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTCATAAT AGATGGAGTC 
TTCAATGGTG ATGCCGTTCA GGTCCAAGAA TACCGTGAAG CCCTGGAGGG GATACTGATC 
AGAGGCAATA ACGGGGTCCA CTTGTTGCCT GAACTCTACG CCATCCCACC TGACAAGGTG 
GATGAAGAGT ATAAGAATCC TCACACGGTG GATAGAGTTC CCCTGGGGAA GGTGCCGCAT 
CTGTGGGGTC AGTCCTTGTA CATCCTCAGC TCCCTGTTGG CAGAGGGATT CCTTGCTGCT 
GGTGAAATTG ATCCCTTGAA CAGAAGATTT TCCACTTCAG TCAAACCTGA TGTCGTCGTA 
CAAGTGACTG TTCTGGCAGA AAACAACCAC ATTAAGGAGC TTCTGAGGAA ACATGGGGTC 
AACGTCCAGA GTATCGCTGA CATTCATCCA ATCCGAGTCC AGCCAGGCCG CATCCTTAGT 
CACATATATG CCAAGCTTGG ACGGAATAAG AATATGAGAT TGAGTGGGCG ACCGTATCGG 
CACATTGGTG TCCTTGGGAC ATCGAAACTA TACGTGATTA GGAACCAGAT CTTTACTTTC 
ACGCCCCAGT TCACCGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATCGTA 
GAGATGCTGA GGATTGAGCT GGCCTACCTG TGCACCTGCT GGCGGATGAC GGGCAGACCC 
ACGCTCACCT TCCCCATCAC GCATACCATG CTCACAAATG ATGGCTCAGA TATTCATTCC 
GCTGTTCTTG CTACAATTCG CAAACTAGAG GATGGATATT TTGGAGGAGC TAGAGTAAAA 
TTAGGGAGTC TTTCGGAATT TCTCACCACC TCTTTCTACA CGTATCTGAC CTTCCTGGAT 
CCAGACTGTG ATGAGAAGTT GTTTGATGAT GCCAGTGAAG GGACCTTCAG TCCTGATAGT 
GATTCAGACC TGGGAGGATA TTTGGAAGAC ACCTATAATC AAGAAAGCCA GGACGAGCTT 
GACCAGTATA TCAACCACCT TCTGCAAAGC ACATCCTCGA AGTGCTATCT GCCACCTCTC 
TGTAAGAAGA CAGAGGACAA CCAGGTTTTC AGTGCTATCC ATTCCACTCG GGATATACTT 
TCTGTGATGG CAAAAGCAAA GGGCTTGGAA ATTCCATTTG TTCCCATGGC TTTGCCAACT 
AAAGTTCTAA GTGCCCACCG TAAATCGCTG AATCTCGTTG ATTCTCCTCA GCCACTCCTA 
AAAAAGGCCC CTGAAGATGA CTTCCAGTTT CCCAGAGATG ACCATGGCGA CGTGGACTGT 
GAGAAGCTGG TTGAGCAGTT GAAAGACTGT TCGAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTGT ATGTAATAAA GGGTCCCAGC TGGGACACAA ATCTCTCTGG ACAGCACGGG 
GTCACTGTTC ACAACCTCCT CAGTGAGCTC TATGCGAAGG CCGGCTTGAA CCAGGAGTGG 
GGCCTGATCC GCTACATCTC CGGCCTCCTC CGGAAGAAAG TGGAGGTCCT GGCTGAGGCG 
TGCACGGACC TGCTGTCACA CCAGAAGCAG CTCACAGTGG GCCTGCCCCC AGAACCCCGG 
GAGAAGACCA TCTCTGCGCC TCTTCCCCCC GAGGAGCTCA CGAAGCTCAT CTACGAAGCC 
AGCGGGCAGG ACATCAGCAT CGCCGTGCTC ACACAGGAGA TCGTGGTGTA CCTGGCGATG 
TACGTCAGGG CACAGCCCAG CCTCTTCGTG GAGATGCTCC GGCTCCGAAT TGGACTGATC 
ATTCAGGTGA TGGCCACGGA GCTGGCACGG AGCCTGAACT GTTCAGGAGA AGAAGCTTCT 
GAAAGTCTGA TGAACCTCAG CCCTTTTGAT ATGAAAAATC TCCTGCACCA CATCTTAAGT 
GGCAAAGAAT TTGGTGTTGA AAGAAGCATG CGGCCCATTC ACTCCTCCAC ACCTAGCCCT 
GCTGTCTCCA TCCATGAGGT GGGCCACACT GGAGTCACCA AAACCGAGAG GAGCGGCATC 
AACAGGCTGA GGAGTGAGAT GAAACAGATG ACTAGGCGGT TTAGTGCTGA TGAACAGCTC 
ATTTCTGTGA GCCAGGCCGC ATCCAGCAGC GCGCGCTCCT CAAAGTCTGC GAGGTCCAGC 
ACCCCATCCT CACCCACGGG CACATCGTCA TCAGACTCGG GCAGGCAGCA CATGGGCTGG 
GGGGAACGGC AGGGCCAGTG GCTGCGCAGG AGAAGGCTGG ACGGCGCCAT CAACAGGGTC 
CCCGTGGGAT TCTACCAGAA GGTGTGGAAG ATCCTTCAGA AGTGCCATGG TCTGTCCATC 
GACGGTTACG TGCTCCCGTC CTCGACCACG CAAGAGATGA CCCCGCAGGA GATCAAGTTT 
GCTGTCCACG TTGAGTCAGT GCTGAACCGA GTGCCACAAC CCGAGTACCG GCAGCTGCTG 
GTGGAGGCCA TCATGGTGCT GACGCTGCTC TCGGACACAG AGATGAACAG CATCGGGGGC 
ATCATCCATG TGGACCAGAT CGTGCACATG GCCAACCAGC TGTTCCTACA AGACCAGGTG 
TCAAGTGGAG CCACGGACAC GCTGGAGAAA GACCAAGCCA CAGGCATCTG CCACTTCTTT 
TACGACAGCG CTCCAAGCGG GGCTTATGGG ACGATGACCT ACCTAACGAA GGCGGTGGCT 
TCTCATTTGC AGGAACTGCT GCCCAATTCA GGTTGCCAGA TGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_024843734.1
CDS	253..3960
Translation	MRSRSNSGVRLDGYARLVQQTILCYQNPITGLMSASHEQKDAWVRDNIYSILAVWGLGMAYRKNADRDEDKAKAYELEQNVVKLMRGLLQCMMRQVDKVERFKHTQSTEDSLHAKYNTATCSTVVGDDEWGHLQVDATSLFLLFLAQMTASGLRIIFTLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGMAKAALEAIDELDLFGAHGGRKSVIHVLPDEVEHCQSILFSMLPRASTSKEIDAGLLSIISFPAFAVEDVNLVNVTKNEIISKLQGRYGCCRFLRDGYKTPREDPNRLHYDPAELKLFENIECEWPVFWTYFIIDGVFNGDAVQVQEYREALEGILIRGNNGVHLLPELYAIPPDKVDEEYKNPHTVDRVPLGKVPHLWGQSLYILSSLLAEGFLAAGEIDPLNRRFSTSVKPDVVVQVTVLAENNHIKELLRKHGVNVQSIADIHPIRVQPGRILSHIYAKLGRNKNMRLSGRPYRHIGVLGTSKLYVIRNQIFTFTPQFTDQHHFYLALDNEMIVEMLRIELAYLCTCWRMTGRPTLTFPITHTMLTNDGSDIHSAVLATIRKLEDGYFGGARVKLGSLSEFLTTSFYTYLTFLDPDCDEKLFDDASEGTFSPDSDSDLGGYLEDTYNQESQDELDQYINHLLQSTSSKCYLPPLCKKTEDNQVFSAIHSTRDILSVMAKAKGLEIPFVPMALPTKVLSAHRKSLNLVDSPQPLLKKAPEDDFQFPRDDHGDVDCEKLVEQLKDCSNLQDQADILYILYVIKGPSWDTNLSGQHGVTVHNLLSELYAKAGLNQEWGLIRYISGLLRKKVEVLAEACTDLLSHQKQLTVGLPPEPREKTISAPLPPEELTKLIYEASGQDISIAVLTQEIVVYLAMYVRAQPSLFVEMLRLRIGLIIQVMATELARSLNCSGEEASESLMNLSPFDMKNLLHHILSGKEFGVERSMRPIHSSTPSPAVSIHEVGHTGVTKTERSGINRLRSEMKQMTRRFSADEQLISVSQAASSSARSSKSARSSTPSSPTGTSSSDSGRQHMGWGERQGQWLRRRRLDGAINRVPVGFYQKVWKILQKCHGLSIDGYVLPSSTTQEMTPQEIKFAVHVESVLNRVPQPEYRQLLVEAIMVLTLLSDTEMNSIGGIIHVDQIVHMANQLFLQDQVSSGATDTLEKDQATGICHFFYDSAPSGAYGTMTYLTKAVASHLQELLPNSGCQMQ

Target ORF information:

RefSeq Version	XM_024987966.1
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphorylase kinase regulatory subunit alpha 2 (PHKA2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_024987966.1

ORF Insert Sequence:

ATGAGGAGCA GGAGCAATTC CGGGGTCCGC TTAGACGGAT ACGCGCGGCT GGTGCAGCAA 
ACAATCCTCT GTTACCAGAA TCCCATCACG GGGCTGATGT CAGCCAGTCA TGAGCAGAAG 
GACGCCTGGG TACGGGATAA CATCTACAGC ATCCTGGCCG TGTGGGGCCT GGGCATGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAAT 
GTGGTGAAAC TGATGCGAGG TCTTCTCCAG TGCATGATGA GACAGGTGGA TAAAGTGGAG 
AGGTTCAAGC ACACTCAGAG TACCGAGGAC AGCCTGCACG CCAAGTACAA CACTGCCACC 
TGCAGCACTG TGGTGGGCGA CGACGAGTGG GGCCACCTCC AGGTGGATGC CACCTCCCTC 
TTCCTCCTGT TTCTGGCCCA GATGACAGCC TCCGGCTTAC GTATTATTTT CACTCTTGAC 
GAGGTGGCCT TCATACAGAA TCTTGTTTTT TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGAATGT GGGAACGTGG AGATAAGACT AATCAGGGTA TTCCAGAATT GAATGCAAGC 
TCTGTAGGAA TGGCCAAGGC AGCCCTTGAA GCAATTGATG AACTGGACCT TTTTGGAGCC 
CATGGAGGTC GCAAGTCTGT GATCCACGTC CTGCCGGATG AGGTGGAACA CTGCCAGTCT 
ATTCTGTTCT CCATGCTGCC AAGAGCCTCG ACGTCAAAAG AGATTGATGC TGGGCTTCTT 
TCCATTATTT CTTTCCCGGC CTTCGCGGTG GAAGATGTGA ACCTTGTGAA TGTGACAAAA 
AATGAAATTA TTTCCAAGCT CCAGGGGCGT TATGGATGCT GTCGCTTCCT TCGAGATGGT 
TATAAAACCC CAAGAGAGGA CCCAAACCGA TTGCATTATG ACCCTGCTGA ACTCAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTCATAAT AGATGGAGTC 
TTCAATGGTG ATGCCGTTCA GGTCCAAGAA TACCGTGAAG CCCTGGAGGG GATACTGATC 
AGAGGCAATA ACGGGGTCCA CTTGTTGCCT GAACTCTACG CCATCCCACC TGACAAGGTG 
GATGAAGAGT ATAAGAATCC TCACACGGTG GATAGAGTTC CCCTGGGGAA GGTGCCGCAT 
CTGTGGGGTC AGTCCTTGTA CATCCTCAGC TCCCTGTTGG CAGAGGGATT CCTTGCTGCT 
GGTGAAATTG ATCCCTTGAA CAGAAGATTT TCCACTTCAG TCAAACCTGA TGTCGTCGTA 
CAAGTGACTG TTCTGGCAGA AAACAACCAC ATTAAGGAGC TTCTGAGGAA ACATGGGGTC 
AACGTCCAGA GTATCGCTGA CATTCATCCA ATCCGAGTCC AGCCAGGCCG CATCCTTAGT 
CACATATATG CCAAGCTTGG ACGGAATAAG AATATGAGAT TGAGTGGGCG ACCGTATCGG 
CACATTGGTG TCCTTGGGAC ATCGAAACTA TACGTGATTA GGAACCAGAT CTTTACTTTC 
ACGCCCCAGT TCACCGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATCGTA 
GAGATGCTGA GGATTGAGCT GGCCTACCTG TGCACCTGCT GGCGGATGAC GGGCAGACCC 
ACGCTCACCT TCCCCATCAC GCATACCATG CTCACAAATG ATGGCTCAGA TATTCATTCC 
GCTGTTCTTG CTACAATTCG CAAACTAGAG GATGGATATT TTGGAGGAGC TAGAGTAAAA 
TTAGGGAGTC TTTCGGAATT TCTCACCACC TCTTTCTACA CGTATCTGAC CTTCCTGGAT 
CCAGACTGTG ATGAGAAGTT GTTTGATGAT GCCAGTGAAG GGACCTTCAG TCCTGATAGT 
GATTCAGACC TGGGAGGATA TTTGGAAGAC ACCTATAATC AAGAAAGCCA GGACGAGCTT 
GACCAGTATA TCAACCACCT TCTGCAAAGC ACATCCTCGA AGTGCTATCT GCCACCTCTC 
TGTAAGAAGA CAGAGGACAA CCAGGTTTTC AGTGCTATCC ATTCCACTCG GGATATACTT 
TCTGTGATGG CAAAAGCAAA GGGCTTGGAA ATTCCATTTG TTCCCATGGC TTTGCCAACT 
AAAGTTCTAA GTGCCCACCG TAAATCGCTG AATCTCGTTG ATTCTCCTCA GCCACTCCTA 
AAAAAGGCCC CTGAAGATGA CTTCCAGTTT CCCAGAGATG ACCATGGCGA CGTGGACTGT 
GAGAAGCTGG TTGAGCAGTT GAAAGACTGT TCGAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTGT ATGTAATAAA GGGTCCCAGC TGGGACACAA ATCTCTCTGG ACAGCACGGG 
GTCACTGTTC ACAACCTCCT CAGTGAGCTC TATGCGAAGG CCGGCTTGAA CCAGGAGTGG 
GGCCTGATCC GCTACATCTC CGGCCTCCTC CGGAAGAAAG TGGAGGTCCT GGCTGAGGCG 
TGCACGGACC TGCTGTCACA CCAGAAGCAG CTCACAGTGG GCCTGCCCCC AGAACCCCGG 
GAGAAGACCA TCTCTGCGCC TCTTCCCCCC GAGGAGCTCA CGAAGCTCAT CTACGAAGCC 
AGCGGGCAGG ACATCAGCAT CGCCGTGCTC ACACAGGAGA TCGTGGTGTA CCTGGCGATG 
TACGTCAGGG CACAGCCCAG CCTCTTCGTG GAGATGCTCC GGCTCCGAAT TGGACTGATC 
ATTCAGGTGA TGGCCACGGA GCTGGCACGG AGCCTGAACT GTTCAGGAGA AGAAGCTTCT 
GAAAGTCTGA TGAACCTCAG CCCTTTTGAT ATGAAAAATC TCCTGCACCA CATCTTAAGT 
GGCAAAGAAT TTGGTGTTGA AAGAAGCATG CGGCCCATTC ACTCCTCCAC ACCTAGCCCT 
GCTGTCTCCA TCCATGAGGT GGGCCACACT GGAGTCACCA AAACCGAGAG GAGCGGCATC 
AACAGGCTGA GGAGTGAGAT GAAACAGATG ACTAGGCGGT TTAGTGCTGA TGAACAGCTC 
ATTTCTGTGA GCCAGGCCGC ATCCAGCAGC GCGCGCTCCT CAAAGTCTGC GAGGTCCAGC 
ACCCCATCCT CACCCACGGG CACATCGTCA TCAGACTCGG GCAGGCAGCA CATGGGCTGG 
GGGGAACGGC AGGGCCAGTG GCTGCGCAGG AGAAGGCTGG ACGGCGCCAT CAACAGGGTC 
CCCGTGGGAT TCTACCAGAA GGTGTGGAAG ATCCTTCAGA AGTGCCATGG TCTGTCCATC 
GACGGTTACG TGCTCCCGTC CTCGACCACG CAAGAGATGA CCCCGCAGGA GATCAAGTTT 
GCTGTCCACG TTGAGTCAGT GCTGAACCGA GTGCCACAAC CCGAGTACCG GCAGCTGCTG 
GTGGAGGCCA TCATGGTGCT GACGCTGCTC TCGGACACAG AGATGAACAG CATCGGGGGC 
ATCATCCATG TGGACCAGAT CGTGCACATG GCCAACCAGC TGTTCCTACA AGACCAGGTG 
TCAAGTGGAG CCACGGACAC GCTGGAGAAA GACCAAGCCA CAGGCATCTG CCACTTCTTT 
TACGACAGCG CTCCAAGCGG GGCTTATGGG ACGATGACCT ACCTAACGAA GGCGGTGGCT 
TCTCATTTGC AGGAACTGCT GCCCAATTCA GGTTGCCAGA TGCAGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA2 ( XM_005228392.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228449.1 Bos taurus phosphorylase kinase, alpha 2 (liver) (PHKA2), transcript variant X4, mRNA.

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CloneID	OBa35761
Clone ID Related Accession (Same CDS sequence)	XM_005228392.3
Accession Version	XM_005228392.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3696bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104772.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228392.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCA GGAGCAATTC CGGGGTCCGC TTAGACGGAT ACGCGCGGCT GGTGCAGCAA 
ACAATCCTCT GTTACCAGAA TCCCATCACG GGGCTGATGT CAGCCAGTCA TGAGCAGAAG 
GACGCCTGGG TACGGGATAA CATCTACAGC ATCCTGGCCG TGTGGGGCCT GGGCATGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAAT 
GTGGTGAAAC TGATGCGAGG TCTTCTCCAG TGCATGATGA GACAGGTGGA TAAAGTGGAG 
AGGTTCAAGC ACACTCAGAG TACCGAGGAC AGCCTGCACG CCAAGTACAA CACTGCCACC 
TGCAGCACTG TGGTGGGCGA CGACGAGTGG GGCCACCTCC AGGTGGATGC CACCTCCCTC 
TTCCTCCTGT TTCTGGCCCA GATGACAGCC TCCGGCTTAC GTATTATTTT CACTCTTGAC 
GAGGTGGCCT TCATACAGAA TCTTGTTTTT TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGAATGT GGGAACGTGG AGATAAGACT AATCAGGGTA TTCCAGAATT GAATGCAAGC 
TCTGTAGGAA TGGCCAAGGC AGCCCTTGAA GCAATTGATG AACTGGACCT TTTTGGAGCC 
CATGGAGGTC GCAAGTCTGT GATCCACGTC CTGCCGGATG AGGTGGAACA CTGCCAGTCT 
ATTCTGTTCT CCATGCTGCC AAGAGCCTCG ACGTCAAAAG AGATTGATGC TGGGCTTCTT 
TCCATTATTT CTTTCCCGGC CTTCGCGGTG GAAGATGTGA ACCTTGTGAA TGTGACAAAA 
AATGAAATTA TTTCCAAGCT CCAGGGGCGT TATGGATGCT GTCGCTTCCT TCGAGATGGT 
TATAAAACCC CAAGAGAGGA CCCAAACCGA TTGCATTATG ACCCTGCTGA ACTCAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTCATAAT AGATGGAGTC 
TTCAATGGTG ATGCCGTTCA GGTCCAAGAA TACCGTGAAG CCCTGGAGGG GATACTGATC 
AGAGGCAATA ACGGGGTCCA CTTGTTGCCT GAACTCTACG CCATCCCACC TGACAAGGTG 
GATGAAGAGT ATAAGAATCC TCACACGGTG GATAGAGTTC CCCTGGGGAA GGTGCCGCAT 
CTGTGGGGTC AGTCCTTGTA CATCCTCAGC TCCCTGTTGG CAGAGGGATT CCTTGCTGCT 
GGTGAAATTG ATCCCTTGAA CAGAAGATTT TCCACTTCAG TCAAACCTGA TGTCGTCGTA 
CAAGTGACTG TTCTGGCAGA AAACAACCAC ATTAAGGAGC TTCTGAGGAA ACATGGGGTC 
AACGTCCAGA GTATCGCTGA CATTCATCCA ATCCGAGTCC AGCCAGGCCG CATCCTTAGT 
CACATATATG CCAAGCTTGG ACGGAATAAG AATATGAGAT TGAGTGGGCG ACCGTATCGG 
CACATTGGTG TCCTTGGGAC ATCGAAACTA TACGTGATTA GGAACCAGAT CTTTACTTTC 
ACGCCCCAGT TCACCGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATCGTA 
GAGATGCTGA GGATTGAGCT GGCCTACCTG TGCACCTGCT GGCGGATGAC GGGCAGACCC 
ACGCTCACCT TCCCCATCAC GCATACCATG CTCACAAATG ATGGCTCAGA TATTCATTCC 
GCTGTTCTTG CTACAATTCG CAAACTAGAG GATGGATATT TTGGAGGAGC TAGAGTAAAA 
TTAGGGAGTC TTTCGGAATT TCTCACCACC TCTTTCTACA CGTATCTGAC CTTCCTGGAT 
CCAGACTGTG ATGAGAAGTT GTTTGATGAT GCCAGTGAAG GGACCTTCAG TCCTGATAGT 
GATTCAGACC TGGGAGGATA TTTGGAAGAC ACCTATAATC AAGAAAGCCA GGACGAGCTT 
GACCAGTATA TCAACCACCT TCTGCAAAGC ACATCCTCGA AGTGCTATCT GCCACCTCTC 
TGTAAGAAGA CAGAGGACAA CCAGGTTTTC AGTGCTATCC ATTCCACTCG GGATATACTT 
TCTGTGATGG CAAAAGCAAA GGGCTTGGAA ATTCCATTTG TTCCCATGGC TTTGCCAACT 
AAAGTTCTAA GTGCCCACCG TAAATCGCTG AATCTCGTTG ATTCTCCTCA GCCACTCCTA 
AAAAAGGCCC CTGAAGATGA CTTCCAGTTT CCCAGAGATG ACCATGGCGA CGTGGACTGT 
GAGAAGCTGG TTGAGCAGTT GAAAGACTGT TCGAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTGT ATGTAATAAA GGGTCCCAGC TGGGACACAA ATCTCTCTGG ACAGCACGGG 
GTCACTGTTC ACAACCTCCT CAGTGAGCTC TATGCGAAGG CCGGCTTGAA CCAGGAGTGG 
GGCCTGATCC GCTACATCTC CGGCCTCCTC CGGAAGAAAG TGGAGGTCCT GGCTGAGGCG 
TGCACGGACC TGCTGTCACA CCAGAAGCAG CTCACAGTGG GCCTGCCCCC AGAACCCCGG 
GAGAAGACCA TCTCTGCGCC TCTTCCCCCC GAGGAGCTCA CGAAGCTCAT CTACGAAGCC 
AGCGGGCAGG ACATCAGCAT CGCCGTGCTC ACACAGGAGA TCGTGGTGTA CCTGGCGATG 
TACGTCAGGG CACAGCCCAG CCTCTTCGTG GAGATGCTCC GGCTCCGAAT TGGACTGATC 
ATTCAGGTGA TGGCCACGGA GCTGGCACGG AGCCTGAACT GTTCAGGAGA AGAAGCTTCT 
GAAAGTCTGA TGAACCTCAG CCCTTTTGAT ATGAAAAATC TCCTGCACCA CATCTTAAGT 
GGCAAAGAAT TTGGTGTTGA AAGAAGCATG CGGCCCATTC ACTCCTCCAC ACCTAGCCCT 
GCTGTCTCCA TCCATGAGGT GGGCCACACT GGAGTCACCA AAACCGAGAG GAGCGGCATC 
AACAGGCTGA GGAGTGAGAT GAAACAGATG ACTAGGCGGT TTAGTGCTGA TGAACAGAGG 
TCCAGCACCC CATCCTCACC CACGGGCACA TCGTCATCAG ACTCGGGCAG GCAGCACATG 
GGCTGGGGGG AACGGCAGGG CCAGTGGCTG CGCAGGAGAA GGCTGGACGG CGCCATCAAC 
AGGGTCCCCG TGGGATTCTA CCAGAAGGTG TGGAAGATCC TTCAGAAGTG CCATGGTCTG 
TCCATCGACG GTTACGTGCT CCCGTCCTCG ACCACGCAAG AGATGACCCC GCAGGAGATC 
AAGTTTGCTG TCCACGTTGA GTCAGTGCTG AACCGAGTGC CACAACCCGA GTACCGGCAG 
CTGCTGGTGG AGGCCATCAT GGTGCTGACG CTGCTCTCGG ACACAGAGAT GAACAGCATC 
GGGGGCATCA TCCATGTGGA CCAGATCGTG CACATGGCCA ACCAGCTGTT CCTACAAGAC 
CAGGTGTCAA GTGGAGCCAC GGACACGCTG GAGAAAGACC AAGCCACAGG CATCTGCCAC 
TTCTTTTACG ACAGCGCTCC AAGCGGGGCT TATGGGACGA TGACCTACCT AACGAAGGCG 
GTGGCTTCTC ATTTGCAGGA ACTGCTGCCC AATTCAGCAT CTGGTCTCCT GGAGTCTGAG 
GACAGATTGG AAGTGGGTCT CTCAAGTGCA GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228449.1
CDS	250..3945
Translation	MRSRSNSGVRLDGYARLVQQTILCYQNPITGLMSASHEQKDAWVRDNIYSILAVWGLGMAYRKNADRDEDKAKAYELEQNVVKLMRGLLQCMMRQVDKVERFKHTQSTEDSLHAKYNTATCSTVVGDDEWGHLQVDATSLFLLFLAQMTASGLRIIFTLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGMAKAALEAIDELDLFGAHGGRKSVIHVLPDEVEHCQSILFSMLPRASTSKEIDAGLLSIISFPAFAVEDVNLVNVTKNEIISKLQGRYGCCRFLRDGYKTPREDPNRLHYDPAELKLFENIECEWPVFWTYFIIDGVFNGDAVQVQEYREALEGILIRGNNGVHLLPELYAIPPDKVDEEYKNPHTVDRVPLGKVPHLWGQSLYILSSLLAEGFLAAGEIDPLNRRFSTSVKPDVVVQVTVLAENNHIKELLRKHGVNVQSIADIHPIRVQPGRILSHIYAKLGRNKNMRLSGRPYRHIGVLGTSKLYVIRNQIFTFTPQFTDQHHFYLALDNEMIVEMLRIELAYLCTCWRMTGRPTLTFPITHTMLTNDGSDIHSAVLATIRKLEDGYFGGARVKLGSLSEFLTTSFYTYLTFLDPDCDEKLFDDASEGTFSPDSDSDLGGYLEDTYNQESQDELDQYINHLLQSTSSKCYLPPLCKKTEDNQVFSAIHSTRDILSVMAKAKGLEIPFVPMALPTKVLSAHRKSLNLVDSPQPLLKKAPEDDFQFPRDDHGDVDCEKLVEQLKDCSNLQDQADILYILYVIKGPSWDTNLSGQHGVTVHNLLSELYAKAGLNQEWGLIRYISGLLRKKVEVLAEACTDLLSHQKQLTVGLPPEPREKTISAPLPPEELTKLIYEASGQDISIAVLTQEIVVYLAMYVRAQPSLFVEMLRLRIGLIIQVMATELARSLNCSGEEASESLMNLSPFDMKNLLHHILSGKEFGVERSMRPIHSSTPSPAVSIHEVGHTGVTKTERSGINRLRSEMKQMTRRFSADEQRSSTPSSPTGTSSSDSGRQHMGWGERQGQWLRRRRLDGAINRVPVGFYQKVWKILQKCHGLSIDGYVLPSSTTQEMTPQEIKFAVHVESVLNRVPQPEYRQLLVEAIMVLTLLSDTEMNSIGGIIHVDQIVHMANQLFLQDQVSSGATDTLEKDQATGICHFFYDSAPSGAYGTMTYLTKAVASHLQELLPNSASGLLESEDRLEVGLSSAE

Target ORF information:

RefSeq Version	XM_005228392.3
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphorylase kinase, alpha 2 (liver) (PHKA2), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228392.3

ORF Insert Sequence:

ATGAGGAGCA GGAGCAATTC CGGGGTCCGC TTAGACGGAT ACGCGCGGCT GGTGCAGCAA 
ACAATCCTCT GTTACCAGAA TCCCATCACG GGGCTGATGT CAGCCAGTCA TGAGCAGAAG 
GACGCCTGGG TACGGGATAA CATCTACAGC ATCCTGGCCG TGTGGGGCCT GGGCATGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAAT 
GTGGTGAAAC TGATGCGAGG TCTTCTCCAG TGCATGATGA GACAGGTGGA TAAAGTGGAG 
AGGTTCAAGC ACACTCAGAG TACCGAGGAC AGCCTGCACG CCAAGTACAA CACTGCCACC 
TGCAGCACTG TGGTGGGCGA CGACGAGTGG GGCCACCTCC AGGTGGATGC CACCTCCCTC 
TTCCTCCTGT TTCTGGCCCA GATGACAGCC TCCGGCTTAC GTATTATTTT CACTCTTGAC 
GAGGTGGCCT TCATACAGAA TCTTGTTTTT TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGAATGT GGGAACGTGG AGATAAGACT AATCAGGGTA TTCCAGAATT GAATGCAAGC 
TCTGTAGGAA TGGCCAAGGC AGCCCTTGAA GCAATTGATG AACTGGACCT TTTTGGAGCC 
CATGGAGGTC GCAAGTCTGT GATCCACGTC CTGCCGGATG AGGTGGAACA CTGCCAGTCT 
ATTCTGTTCT CCATGCTGCC AAGAGCCTCG ACGTCAAAAG AGATTGATGC TGGGCTTCTT 
TCCATTATTT CTTTCCCGGC CTTCGCGGTG GAAGATGTGA ACCTTGTGAA TGTGACAAAA 
AATGAAATTA TTTCCAAGCT CCAGGGGCGT TATGGATGCT GTCGCTTCCT TCGAGATGGT 
TATAAAACCC CAAGAGAGGA CCCAAACCGA TTGCATTATG ACCCTGCTGA ACTCAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTCATAAT AGATGGAGTC 
TTCAATGGTG ATGCCGTTCA GGTCCAAGAA TACCGTGAAG CCCTGGAGGG GATACTGATC 
AGAGGCAATA ACGGGGTCCA CTTGTTGCCT GAACTCTACG CCATCCCACC TGACAAGGTG 
GATGAAGAGT ATAAGAATCC TCACACGGTG GATAGAGTTC CCCTGGGGAA GGTGCCGCAT 
CTGTGGGGTC AGTCCTTGTA CATCCTCAGC TCCCTGTTGG CAGAGGGATT CCTTGCTGCT 
GGTGAAATTG ATCCCTTGAA CAGAAGATTT TCCACTTCAG TCAAACCTGA TGTCGTCGTA 
CAAGTGACTG TTCTGGCAGA AAACAACCAC ATTAAGGAGC TTCTGAGGAA ACATGGGGTC 
AACGTCCAGA GTATCGCTGA CATTCATCCA ATCCGAGTCC AGCCAGGCCG CATCCTTAGT 
CACATATATG CCAAGCTTGG ACGGAATAAG AATATGAGAT TGAGTGGGCG ACCGTATCGG 
CACATTGGTG TCCTTGGGAC ATCGAAACTA TACGTGATTA GGAACCAGAT CTTTACTTTC 
ACGCCCCAGT TCACCGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATCGTA 
GAGATGCTGA GGATTGAGCT GGCCTACCTG TGCACCTGCT GGCGGATGAC GGGCAGACCC 
ACGCTCACCT TCCCCATCAC GCATACCATG CTCACAAATG ATGGCTCAGA TATTCATTCC 
GCTGTTCTTG CTACAATTCG CAAACTAGAG GATGGATATT TTGGAGGAGC TAGAGTAAAA 
TTAGGGAGTC TTTCGGAATT TCTCACCACC TCTTTCTACA CGTATCTGAC CTTCCTGGAT 
CCAGACTGTG ATGAGAAGTT GTTTGATGAT GCCAGTGAAG GGACCTTCAG TCCTGATAGT 
GATTCAGACC TGGGAGGATA TTTGGAAGAC ACCTATAATC AAGAAAGCCA GGACGAGCTT 
GACCAGTATA TCAACCACCT TCTGCAAAGC ACATCCTCGA AGTGCTATCT GCCACCTCTC 
TGTAAGAAGA CAGAGGACAA CCAGGTTTTC AGTGCTATCC ATTCCACTCG GGATATACTT 
TCTGTGATGG CAAAAGCAAA GGGCTTGGAA ATTCCATTTG TTCCCATGGC TTTGCCAACT 
AAAGTTCTAA GTGCCCACCG TAAATCGCTG AATCTCGTTG ATTCTCCTCA GCCACTCCTA 
AAAAAGGCCC CTGAAGATGA CTTCCAGTTT CCCAGAGATG ACCATGGCGA CGTGGACTGT 
GAGAAGCTGG TTGAGCAGTT GAAAGACTGT TCGAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTGT ATGTAATAAA GGGTCCCAGC TGGGACACAA ATCTCTCTGG ACAGCACGGG 
GTCACTGTTC ACAACCTCCT CAGTGAGCTC TATGCGAAGG CCGGCTTGAA CCAGGAGTGG 
GGCCTGATCC GCTACATCTC CGGCCTCCTC CGGAAGAAAG TGGAGGTCCT GGCTGAGGCG 
TGCACGGACC TGCTGTCACA CCAGAAGCAG CTCACAGTGG GCCTGCCCCC AGAACCCCGG 
GAGAAGACCA TCTCTGCGCC TCTTCCCCCC GAGGAGCTCA CGAAGCTCAT CTACGAAGCC 
AGCGGGCAGG ACATCAGCAT CGCCGTGCTC ACACAGGAGA TCGTGGTGTA CCTGGCGATG 
TACGTCAGGG CACAGCCCAG CCTCTTCGTG GAGATGCTCC GGCTCCGAAT TGGACTGATC 
ATTCAGGTGA TGGCCACGGA GCTGGCACGG AGCCTGAACT GTTCAGGAGA AGAAGCTTCT 
GAAAGTCTGA TGAACCTCAG CCCTTTTGAT ATGAAAAATC TCCTGCACCA CATCTTAAGT 
GGCAAAGAAT TTGGTGTTGA AAGAAGCATG CGGCCCATTC ACTCCTCCAC ACCTAGCCCT 
GCTGTCTCCA TCCATGAGGT GGGCCACACT GGAGTCACCA AAACCGAGAG GAGCGGCATC 
AACAGGCTGA GGAGTGAGAT GAAACAGATG ACTAGGCGGT TTAGTGCTGA TGAACAGAGG 
TCCAGCACCC CATCCTCACC CACGGGCACA TCGTCATCAG ACTCGGGCAG GCAGCACATG 
GGCTGGGGGG AACGGCAGGG CCAGTGGCTG CGCAGGAGAA GGCTGGACGG CGCCATCAAC 
AGGGTCCCCG TGGGATTCTA CCAGAAGGTG TGGAAGATCC TTCAGAAGTG CCATGGTCTG 
TCCATCGACG GTTACGTGCT CCCGTCCTCG ACCACGCAAG AGATGACCCC GCAGGAGATC 
AAGTTTGCTG TCCACGTTGA GTCAGTGCTG AACCGAGTGC CACAACCCGA GTACCGGCAG 
CTGCTGGTGG AGGCCATCAT GGTGCTGACG CTGCTCTCGG ACACAGAGAT GAACAGCATC 
GGGGGCATCA TCCATGTGGA CCAGATCGTG CACATGGCCA ACCAGCTGTT CCTACAAGAC 
CAGGTGTCAA GTGGAGCCAC GGACACGCTG GAGAAAGACC AAGCCACAGG CATCTGCCAC 
TTCTTTTACG ACAGCGCTCC AAGCGGGGCT TATGGGACGA TGACCTACCT AACGAAGGCG 
GTGGCTTCTC ATTTGCAGGA ACTGCTGCCC AATTCAGCAT CTGGTCTCCT GGAGTCTGAG 
GACAGATTGG AAGTGGGTCT CTCAAGTGCA GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA2 ( XM_005228393.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228450.1 Bos taurus phosphorylase kinase, alpha 2 (liver) (PHKA2), transcript variant X5, mRNA.

Price & Availability↑

CloneID	OBa35762
Clone ID Related Accession (Same CDS sequence)	XM_005228393.3
Accession Version	XM_005228393.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3666bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104772.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228393.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCA GGAGCAATTC CGGGGTCCGC TTAGACGGAT ACGCGCGGCT GGTGCAGCAA 
ACAATCCTCT GTTACCAGAA TCCCATCACG GGGCTGATGT CAGCCAGTCA TGAGCAGAAG 
GACGCCTGGG TACGGGATAA CATCTACAGC ATCCTGGCCG TGTGGGGCCT GGGCATGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAAT 
GTGGTGAAAC TGATGCGAGG TCTTCTCCAG TGCATGATGA GACAGGTGGA TAAAGTGGAG 
AGGTTCAAGC ACACTCAGAG TACCGAGGAC AGCCTGCACG CCAAGTACAA CACTGCCACC 
TGCAGCACTG TGGTGGGCGA CGACGAGTGG GGCCACCTCC AGGTGGATGC CACCTCCCTC 
TTCCTCCTGT TTCTGGCCCA GATGACAGCC TCCGGCTTAC GTATTATTTT CACTCTTGAC 
GAGGTGGCCT TCATACAGAA TCTTGTTTTT TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGAATGT GGGAACGTGG AGATAAGACT AATCAGGGTA TTCCAGAATT GAATGCAAGC 
TCTGTAGGAA TGGCCAAGGC AGCCCTTGAA GCAATTGATG AACTGGACCT TTTTGGAGCC 
CATGGAGGTC GCAAGTCTGT GATCCACGTC CTGCCGGATG AGGTGGAACA CTGCCAGTCT 
ATTCTGTTCT CCATGCTGCC AAGAGCCTCG ACGTCAAAAG AGATTGATGC TGGGCTTCTT 
TCCATTATTT CTTTCCCGGC CTTCGCGGTG GAAGATGTGA ACCTTGTGAA TGTGACAAAA 
AATGAAATTA TTTCCAAGCT CCAGGGGCGT TATGGATGCT GTCGCTTCCT TCGAGATGGT 
TATAAAACCC CAAGAGAGGA CCCAAACCGA TTGCATTATG ACCCTGCTGA ACTCAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTCATAAT AGATGGAGTC 
TTCAATGGTG ATGCCGTTCA GGTCCAAGAA TACCGTGAAG CCCTGGAGGG GATACTGATC 
AGAGGCAATA ACGGGGTCCA CTTGTTGCCT GAACTCTACG CCATCCCACC TGACAAGGTG 
GATGAAGAGT ATAAGAATCC TCACACGGTG GATAGAGTTC CCCTGGGGAA GGTGCCGCAT 
CTGTGGGGTC AGTCCTTGTA CATCCTCAGC TCCCTGTTGG CAGAGGGATT CCTTGCTGCT 
GGTGAAATTG ATCCCTTGAA CAGAAGATTT TCCACTTCAG TCAAACCTGA TGTCGTCGTA 
CAAGTGACTG TTCTGGCAGA AAACAACCAC ATTAAGGAGC TTCTGAGGAA ACATGGGGTC 
AACGTCCAGA GTATCGCTGA CATTCATCCA ATCCGAGTCC AGCCAGGCCG CATCCTTAGT 
CACATATATG CCAAGCTTGG ACGGAATAAG AATATGAGAT TGAGTGGGCG ACCGTATCGG 
CACATTGGTG TCCTTGGGAC ATCGAAACTA TACGTGATTA GGAACCAGAT CTTTACTTTC 
ACGCCCCAGT TCACCGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATCGTA 
GAGATGCTGA GGATTGAGCT GGCCTACCTG TGCACCTGCT GGCGGATGAC GGGCAGACCC 
ACGCTCACCT TCCCCATCAC GCATACCATG CTCACAAATG ATGGCTCAGA TATTCATTCC 
GCTGTTCTTG CTACAATTCG CAAACTAGAG GATGGATATT TTGGAGGAGC TAGAGTAAAA 
TTAGGGAGTC TTTCGGAATT TCTCACCACC TCTTTCTACA CGTATCTGAC CTTCCTGGAT 
CCAGACTGTG ATGAGAAGTT GTTTGATGAT GCCAGTGAAG GGACCTTCAG TCCTGATAGT 
GATTCAGACC TGGGAGGATA TTTGGAAGAC ACCTATAATC AAGAAAGCCA GGACGAGCTT 
GACCAGTATA TCAACCACCT TCTGCAAAGC ACATCCTCGA AGTGCTATCT GCCACCTCTC 
TGTAAGAAGA CAGAGGACAA CCAGGTTTTC AGTGCTATCC ATTCCACTCG GGATATACTT 
TCTGTGATGG CAAAAGCAAA GGGCTTGGAA ATTCCATTTG TTCCCATGGC TTTGCCAACT 
AAAGTTCTAA GTGCCCACCG TAAATCGCTG AATCTCGTTG ATTCTCCTCA GCCACTCCTA 
AAAAAGGCCC CTGAAGATGA CTTCCAGTTT CCCAGAGATG ACCATGGCGA CGTGGACTGT 
GAGAAGCTGG TTGAGCAGTT GAAAGACTGT TCGAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTGT ATGTAATAAA GGGTCCCAGC TGGGACACAA ATCTCTCTGG ACAGCACGGG 
GTCACTGTTC ACAACCTCCT CAGTGAGCTC TATGCGAAGG CCGGCTTGAA CCAGGAGTGG 
GGCCTGATCC GCTACATCTC CGGCCTCCTC CGGAAGAAAG TGGAGGTCCT GGCTGAGGCG 
TGCACGGACC TGCTGTCACA CCAGAAGCAG CTCACAGTGG GCCTGCCCCC AGAACCCCGG 
GAGAAGACCA TCTCTGCGCC TCTTCCCCCC GAGGAGCTCA CGAAGCTCAT CTACGAAGCC 
AGCGGGCAGG ACATCAGCAT CGCCGTGCTC ACACAGGAGA TCGTGGTGTA CCTGGCGATG 
TACGTCAGGG CACAGCCCAG CCTCTTCGTG GAGATGCTCC GGCTCCGAAT TGGACTGATC 
ATTCAGGTGA TGGCCACGGA GCTGGCACGG AGCCTGAACT GTTCAGGAGA AGAAGCTTCT 
GAAAGTCTGA TGAACCTCAG CCCTTTTGAT ATGAAAAATC TCCTGCACCA CATCTTAAGT 
GGCAAAGAAT TTGGTGTTGA AAGAAGCATG CGGCCCATTC ACTCCTCCAC ACCTAGCCCT 
GCTGTCTCCA TCCATGAGGT GGGCCACACT GGAGTCACCA AAACCGAGAG GAGCGGCATC 
AACAGGCTGA GGAGTGAGAT GAAACAGAGG TCCAGCACCC CATCCTCACC CACGGGCACA 
TCGTCATCAG ACTCGGGCAG GCAGCACATG GGCTGGGGGG AACGGCAGGG CCAGTGGCTG 
CGCAGGAGAA GGCTGGACGG CGCCATCAAC AGGGTCCCCG TGGGATTCTA CCAGAAGGTG 
TGGAAGATCC TTCAGAAGTG CCATGGTCTG TCCATCGACG GTTACGTGCT CCCGTCCTCG 
ACCACGCAAG AGATGACCCC GCAGGAGATC AAGTTTGCTG TCCACGTTGA GTCAGTGCTG 
AACCGAGTGC CACAACCCGA GTACCGGCAG CTGCTGGTGG AGGCCATCAT GGTGCTGACG 
CTGCTCTCGG ACACAGAGAT GAACAGCATC GGGGGCATCA TCCATGTGGA CCAGATCGTG 
CACATGGCCA ACCAGCTGTT CCTACAAGAC CAGGTGTCAA GTGGAGCCAC GGACACGCTG 
GAGAAAGACC AAGCCACAGG CATCTGCCAC TTCTTTTACG ACAGCGCTCC AAGCGGGGCT 
TATGGGACGA TGACCTACCT AACGAAGGCG GTGGCTTCTC ATTTGCAGGA ACTGCTGCCC 
AATTCAGCAT CTGGTCTCCT GGAGTCTGAG GACAGATTGG AAGTGGGTCT CTCAAGTGCA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228450.1
CDS	250..3915
Translation	MRSRSNSGVRLDGYARLVQQTILCYQNPITGLMSASHEQKDAWVRDNIYSILAVWGLGMAYRKNADRDEDKAKAYELEQNVVKLMRGLLQCMMRQVDKVERFKHTQSTEDSLHAKYNTATCSTVVGDDEWGHLQVDATSLFLLFLAQMTASGLRIIFTLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGMAKAALEAIDELDLFGAHGGRKSVIHVLPDEVEHCQSILFSMLPRASTSKEIDAGLLSIISFPAFAVEDVNLVNVTKNEIISKLQGRYGCCRFLRDGYKTPREDPNRLHYDPAELKLFENIECEWPVFWTYFIIDGVFNGDAVQVQEYREALEGILIRGNNGVHLLPELYAIPPDKVDEEYKNPHTVDRVPLGKVPHLWGQSLYILSSLLAEGFLAAGEIDPLNRRFSTSVKPDVVVQVTVLAENNHIKELLRKHGVNVQSIADIHPIRVQPGRILSHIYAKLGRNKNMRLSGRPYRHIGVLGTSKLYVIRNQIFTFTPQFTDQHHFYLALDNEMIVEMLRIELAYLCTCWRMTGRPTLTFPITHTMLTNDGSDIHSAVLATIRKLEDGYFGGARVKLGSLSEFLTTSFYTYLTFLDPDCDEKLFDDASEGTFSPDSDSDLGGYLEDTYNQESQDELDQYINHLLQSTSSKCYLPPLCKKTEDNQVFSAIHSTRDILSVMAKAKGLEIPFVPMALPTKVLSAHRKSLNLVDSPQPLLKKAPEDDFQFPRDDHGDVDCEKLVEQLKDCSNLQDQADILYILYVIKGPSWDTNLSGQHGVTVHNLLSELYAKAGLNQEWGLIRYISGLLRKKVEVLAEACTDLLSHQKQLTVGLPPEPREKTISAPLPPEELTKLIYEASGQDISIAVLTQEIVVYLAMYVRAQPSLFVEMLRLRIGLIIQVMATELARSLNCSGEEASESLMNLSPFDMKNLLHHILSGKEFGVERSMRPIHSSTPSPAVSIHEVGHTGVTKTERSGINRLRSEMKQRSSTPSSPTGTSSSDSGRQHMGWGERQGQWLRRRRLDGAINRVPVGFYQKVWKILQKCHGLSIDGYVLPSSTTQEMTPQEIKFAVHVESVLNRVPQPEYRQLLVEAIMVLTLLSDTEMNSIGGIIHVDQIVHMANQLFLQDQVSSGATDTLEKDQATGICHFFYDSAPSGAYGTMTYLTKAVASHLQELLPNSASGLLESEDRLEVGLSSAE

Target ORF information:

RefSeq Version	XM_005228393.3
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphorylase kinase, alpha 2 (liver) (PHKA2), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228393.3

ORF Insert Sequence:

ATGAGGAGCA GGAGCAATTC CGGGGTCCGC TTAGACGGAT ACGCGCGGCT GGTGCAGCAA 
ACAATCCTCT GTTACCAGAA TCCCATCACG GGGCTGATGT CAGCCAGTCA TGAGCAGAAG 
GACGCCTGGG TACGGGATAA CATCTACAGC ATCCTGGCCG TGTGGGGCCT GGGCATGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAAT 
GTGGTGAAAC TGATGCGAGG TCTTCTCCAG TGCATGATGA GACAGGTGGA TAAAGTGGAG 
AGGTTCAAGC ACACTCAGAG TACCGAGGAC AGCCTGCACG CCAAGTACAA CACTGCCACC 
TGCAGCACTG TGGTGGGCGA CGACGAGTGG GGCCACCTCC AGGTGGATGC CACCTCCCTC 
TTCCTCCTGT TTCTGGCCCA GATGACAGCC TCCGGCTTAC GTATTATTTT CACTCTTGAC 
GAGGTGGCCT TCATACAGAA TCTTGTTTTT TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGAATGT GGGAACGTGG AGATAAGACT AATCAGGGTA TTCCAGAATT GAATGCAAGC 
TCTGTAGGAA TGGCCAAGGC AGCCCTTGAA GCAATTGATG AACTGGACCT TTTTGGAGCC 
CATGGAGGTC GCAAGTCTGT GATCCACGTC CTGCCGGATG AGGTGGAACA CTGCCAGTCT 
ATTCTGTTCT CCATGCTGCC AAGAGCCTCG ACGTCAAAAG AGATTGATGC TGGGCTTCTT 
TCCATTATTT CTTTCCCGGC CTTCGCGGTG GAAGATGTGA ACCTTGTGAA TGTGACAAAA 
AATGAAATTA TTTCCAAGCT CCAGGGGCGT TATGGATGCT GTCGCTTCCT TCGAGATGGT 
TATAAAACCC CAAGAGAGGA CCCAAACCGA TTGCATTATG ACCCTGCTGA ACTCAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTCATAAT AGATGGAGTC 
TTCAATGGTG ATGCCGTTCA GGTCCAAGAA TACCGTGAAG CCCTGGAGGG GATACTGATC 
AGAGGCAATA ACGGGGTCCA CTTGTTGCCT GAACTCTACG CCATCCCACC TGACAAGGTG 
GATGAAGAGT ATAAGAATCC TCACACGGTG GATAGAGTTC CCCTGGGGAA GGTGCCGCAT 
CTGTGGGGTC AGTCCTTGTA CATCCTCAGC TCCCTGTTGG CAGAGGGATT CCTTGCTGCT 
GGTGAAATTG ATCCCTTGAA CAGAAGATTT TCCACTTCAG TCAAACCTGA TGTCGTCGTA 
CAAGTGACTG TTCTGGCAGA AAACAACCAC ATTAAGGAGC TTCTGAGGAA ACATGGGGTC 
AACGTCCAGA GTATCGCTGA CATTCATCCA ATCCGAGTCC AGCCAGGCCG CATCCTTAGT 
CACATATATG CCAAGCTTGG ACGGAATAAG AATATGAGAT TGAGTGGGCG ACCGTATCGG 
CACATTGGTG TCCTTGGGAC ATCGAAACTA TACGTGATTA GGAACCAGAT CTTTACTTTC 
ACGCCCCAGT TCACCGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATCGTA 
GAGATGCTGA GGATTGAGCT GGCCTACCTG TGCACCTGCT GGCGGATGAC GGGCAGACCC 
ACGCTCACCT TCCCCATCAC GCATACCATG CTCACAAATG ATGGCTCAGA TATTCATTCC 
GCTGTTCTTG CTACAATTCG CAAACTAGAG GATGGATATT TTGGAGGAGC TAGAGTAAAA 
TTAGGGAGTC TTTCGGAATT TCTCACCACC TCTTTCTACA CGTATCTGAC CTTCCTGGAT 
CCAGACTGTG ATGAGAAGTT GTTTGATGAT GCCAGTGAAG GGACCTTCAG TCCTGATAGT 
GATTCAGACC TGGGAGGATA TTTGGAAGAC ACCTATAATC AAGAAAGCCA GGACGAGCTT 
GACCAGTATA TCAACCACCT TCTGCAAAGC ACATCCTCGA AGTGCTATCT GCCACCTCTC 
TGTAAGAAGA CAGAGGACAA CCAGGTTTTC AGTGCTATCC ATTCCACTCG GGATATACTT 
TCTGTGATGG CAAAAGCAAA GGGCTTGGAA ATTCCATTTG TTCCCATGGC TTTGCCAACT 
AAAGTTCTAA GTGCCCACCG TAAATCGCTG AATCTCGTTG ATTCTCCTCA GCCACTCCTA 
AAAAAGGCCC CTGAAGATGA CTTCCAGTTT CCCAGAGATG ACCATGGCGA CGTGGACTGT 
GAGAAGCTGG TTGAGCAGTT GAAAGACTGT TCGAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTGT ATGTAATAAA GGGTCCCAGC TGGGACACAA ATCTCTCTGG ACAGCACGGG 
GTCACTGTTC ACAACCTCCT CAGTGAGCTC TATGCGAAGG CCGGCTTGAA CCAGGAGTGG 
GGCCTGATCC GCTACATCTC CGGCCTCCTC CGGAAGAAAG TGGAGGTCCT GGCTGAGGCG 
TGCACGGACC TGCTGTCACA CCAGAAGCAG CTCACAGTGG GCCTGCCCCC AGAACCCCGG 
GAGAAGACCA TCTCTGCGCC TCTTCCCCCC GAGGAGCTCA CGAAGCTCAT CTACGAAGCC 
AGCGGGCAGG ACATCAGCAT CGCCGTGCTC ACACAGGAGA TCGTGGTGTA CCTGGCGATG 
TACGTCAGGG CACAGCCCAG CCTCTTCGTG GAGATGCTCC GGCTCCGAAT TGGACTGATC 
ATTCAGGTGA TGGCCACGGA GCTGGCACGG AGCCTGAACT GTTCAGGAGA AGAAGCTTCT 
GAAAGTCTGA TGAACCTCAG CCCTTTTGAT ATGAAAAATC TCCTGCACCA CATCTTAAGT 
GGCAAAGAAT TTGGTGTTGA AAGAAGCATG CGGCCCATTC ACTCCTCCAC ACCTAGCCCT 
GCTGTCTCCA TCCATGAGGT GGGCCACACT GGAGTCACCA AAACCGAGAG GAGCGGCATC 
AACAGGCTGA GGAGTGAGAT GAAACAGAGG TCCAGCACCC CATCCTCACC CACGGGCACA 
TCGTCATCAG ACTCGGGCAG GCAGCACATG GGCTGGGGGG AACGGCAGGG CCAGTGGCTG 
CGCAGGAGAA GGCTGGACGG CGCCATCAAC AGGGTCCCCG TGGGATTCTA CCAGAAGGTG 
TGGAAGATCC TTCAGAAGTG CCATGGTCTG TCCATCGACG GTTACGTGCT CCCGTCCTCG 
ACCACGCAAG AGATGACCCC GCAGGAGATC AAGTTTGCTG TCCACGTTGA GTCAGTGCTG 
AACCGAGTGC CACAACCCGA GTACCGGCAG CTGCTGGTGG AGGCCATCAT GGTGCTGACG 
CTGCTCTCGG ACACAGAGAT GAACAGCATC GGGGGCATCA TCCATGTGGA CCAGATCGTG 
CACATGGCCA ACCAGCTGTT CCTACAAGAC CAGGTGTCAA GTGGAGCCAC GGACACGCTG 
GAGAAAGACC AAGCCACAGG CATCTGCCAC TTCTTTTACG ACAGCGCTCC AAGCGGGGCT 
TATGGGACGA TGACCTACCT AACGAAGGCG GTGGCTTCTC ATTTGCAGGA ACTGCTGCCC 
AATTCAGCAT CTGGTCTCCT GGAGTCTGAG GACAGATTGG AAGTGGGTCT CTCAAGTGCA 
GAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PHKA2 ( XM_005228394.3 ) cDNA ORF clone, Bos taurus(cattle) -> XP_005228451.1 Bos taurus phosphorylase kinase, alpha 2 (liver) (PHKA2), transcript variant X6, mRNA.

Price & Availability↑

CloneID	OBa35763
Clone ID Related Accession (Same CDS sequence)	XM_005228394.3
Accession Version	XM_005228394.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3648bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-25
Organism	Bos taurus(cattle)
Product	phosphorylase b kinase regulatory subunit alpha, liver isoform isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_003104772.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jan 26, 2016 this sequence version replaced XM_005228394.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Bos taurus Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGGAGCA GGAGCAATTC CGGGGTCCGC TTAGACGGAT ACGCGCGGCT GGTGCAGCAA 
ACAATCCTCT GTTACCAGAA TCCCATCACG GGGCTGATGT CAGCCAGTCA TGAGCAGAAG 
GACGCCTGGG TACGGGATAA CATCTACAGC ATCCTGGCCG TGTGGGGCCT GGGCATGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAAT 
GTGGTGAAAC TGATGCGAGG TCTTCTCCAG TGCATGATGA GACAGGTGGA TAAAGTGGAG 
AGGTTCAAGC ACACTCAGAG TACCGAGGAC AGCCTGCACG CCAAGTACAA CACTGCCACC 
TGCAGCACTG TGGTGGGCGA CGACGAGTGG GGCCACCTCC AGGTGGATGC CACCTCCCTC 
TTCCTCCTGT TTCTGGCCCA GATGACAGCC TCCGGCTTAC GTATTATTTT CACTCTTGAC 
GAGGTGGCCT TCATACAGAA TCTTGTTTTT TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGAATGT GGGAACGTGG AGATAAGACT AATCAGGGTA TTCCAGAATT GAATGCAAGC 
TCTGTAGGAA TGGCCAAGGC AGCCCTTGAA GCAATTGATG AACTGGACCT TTTTGGAGCC 
CATGGAGGTC GCAAGTCTGT GATCCACGTC CTGCCGGATG AGGTGGAACA CTGCCAGTCT 
ATTCTGTTCT CCATGCTGCC AAGAGCCTCG ACGTCAAAAG AGATTGATGC TGGGCTTCTT 
TCCATTATTT CTTTCCCGGC CTTCGCGGTG GAAGATGTGA ACCTTGTGAA TGTGACAAAA 
AATGAAATTA TTTCCAAGCT CCAGGGGCGT TATGGATGCT GTCGCTTCCT TCGAGATGGT 
TATAAAACCC CAAGAGAGGA CCCAAACCGA TTGCATTATG ACCCTGCTGA ACTCAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTCATAAT AGATGGAGTC 
TTCAATGGTG ATGCCGTTCA GGTCCAAGAA TACCGTGAAG CCCTGGAGGG GATACTGATC 
AGAGGCAATA ACGGGGTCCA CTTGTTGCCT GAACTCTACG CCATCCCACC TGACAAGGTG 
GATGAAGAGT ATAAGAATCC TCACACGGTG GATAGAGTTC CCCTGGGGAA GGTGCCGCAT 
CTGTGGGGTC AGTCCTTGTA CATCCTCAGC TCCCTGTTGG CAGAGGGATT CCTTGCTGCT 
GGTGAAATTG ATCCCTTGAA CAGAAGATTT TCCACTTCAG TCAAACCTGA TGTCGTCGTA 
CAAGTGACTG TTCTGGCAGA AAACAACCAC ATTAAGGAGC TTCTGAGGAA ACATGGGGTC 
AACGTCCAGA GTATCGCTGA CATTCATCCA ATCCGAGTCC AGCCAGGCCG CATCCTTAGT 
CACATATATG CCAAGCTTGG ACGGAATAAG AATATGAGAT TGAGTGGGCG ACCGTATCGG 
CACATTGGTG TCCTTGGGAC ATCGAAACTA TACGTGATTA GGAACCAGAT CTTTACTTTC 
ACGCCCCAGT TCACCGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATCGTA 
GAGATGCTGA GGATTGAGCT GGCCTACCTG TGCACCTGCT GGCGGATGAC GGGCAGACCC 
ACGCTCACCT TCCCCATCAC GCATACCATG CTCACAAATG ATGGCTCAGA TATTCATTCC 
GCTGTTCTTG CTACAATTCG CAAACTAGAG GATGGATATT TTGGAGGAGC TAGAGTAAAA 
TTAGGGAGTC TTTCGGAATT TCTCACCACC TCTTTCTACA CGTATCTGAC CTTCCTGGAT 
CCAGACTGTG ATGAGAAGTT GTTTGATGAT GCCAGTGAAG GGACCTTCAG TCCTGATAGT 
GATTCAGACC TGGGAGGATA TTTGGAAGAC ACCTATAATC AAGAAAGCCA GGACGAGCTT 
GACCAGTATA TCAACCACCT TCTGCAAAGC ACATCCTCGA AGTGCTATCT GCCACCTCTC 
TGTAAGAAGA CAGAGGACAA CCAGGTTTTC AGTGCTATCC ATTCCACTCG GGATATACTT 
TCTGTGATGG CAAAAGCAAA GGGCTTGGAA ATTCCATTTG TTCCCATGGC TTTGCCAACT 
AAAGTTCTAA GTGCCCACCG TAAATCGCTG AATCTCGTTG ATTCTCCTCA GCCACTCCTA 
AAAAAGGCCC CTGAAGATGA CTTCCAGTTT CCCAGAGATG ACCATGGCGA CGTGGACTGT 
GAGAAGCTGG TTGAGCAGTT GAAAGACTGT TCGAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTGT ATGTAATAAA GGGTCCCAGC TGGGACACAA ATCTCTCTGG ACAGCACGGG 
GTCACTGTTC ACAACCTCCT CAGTGAGCTC TATGCGAAGG CCGGCTTGAA CCAGGAGTGG 
GGCCTGATCC GCTACATCTC CGGCCTCCTC CGGAAGAAAG TGGAGGTCCT GGCTGAGGCG 
TGCACGGACC TGCTGTCACA CCAGAAGCAG CTCACAGTGG GCCTGCCCCC AGAACCCCGG 
GAGAAGACCA TCTCTGCGCC TCTTCCCCCC GAGGAGCTCA CGAAGCTCAT CTACGAAGCC 
AGCGGGCAGG ACATCAGCAT CGCCGTGCTC ACACAGGAGA TCGTGGTGTA CCTGGCGATG 
TACGTCAGGG CACAGCCCAG CCTCTTCGTG GAGATGCTCC GGCTCCGAAT TGGACTGATC 
ATTCAGGTGA TGGCCACGGA GCTGGCACGG AGCCTGAACT GTTCAGTGCG GCCCATTCAC 
TCCTCCACAC CTAGCCCTGC TGTCTCCATC CATGAGGTGG GCCACACTGG AGTCACCAAA 
ACCGAGAGGA GCGGCATCAA CAGGCTGAGG AGTGAGATGA AACAGATGAC TAGGCGGTTT 
AGTGCTGATG AACAGCTCAT TTCTGTGAGC CAGGCCGCAT CCAGCAGCGC GCGCTCCTCA 
AAGTCTGCGA GGTCCAGCAC CCCATCCTCA CCCACGGGCA CATCGTCATC AGACTCGGGC 
AGGCAGCACA TGGGCTGGGG GGAACGGCAG GGCCAGTGGC TGCGCAGGAG AAGGCTGGAC 
GGCGCCATCA ACAGGGTCCC CGTGGGATTC TACCAGAAGG TGTGGAAGAT CCTTCAGAAG 
TGCCATGGTC TGTCCATCGA CGGTTACGTG CTCCCGTCCT CGACCACGCA AGAGATGACC 
CCGCAGGAGA TCAAGTTTGC TGTCCACGTT GAGTCAGTGC TGAACCGAGT GCCACAACCC 
GAGTACCGGC AGCTGCTGGT GGAGGCCATC ATGGTGCTGA CGCTGCTCTC GGACACAGAG 
ATGAACAGCA TCGGGGGCAT CATCCATGTG GACCAGATCG TGCACATGGC CAACCAGCTG 
TTCCTACAAG ACCAGGTGTC AAGTGGAGCC ACGGACACGC TGGAGAAAGA CCAAGCCACA 
GGCATCTGCC ACTTCTTTTA CGACAGCGCT CCAAGCGGGG CTTATGGGAC GATGACCTAC 
CTAACGAAGG CGGTGGCTTC TCATTTGCAG GAACTGCTGC CCAATTCAGC ATCTGGTCTC 
CTGGAGTCTG AGGACAGATT GGAAGTGGGT CTCTCAAGTG CAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005228451.1
CDS	251..3898
Translation	MRSRSNSGVRLDGYARLVQQTILCYQNPITGLMSASHEQKDAWVRDNIYSILAVWGLGMAYRKNADRDEDKAKAYELEQNVVKLMRGLLQCMMRQVDKVERFKHTQSTEDSLHAKYNTATCSTVVGDDEWGHLQVDATSLFLLFLAQMTASGLRIIFTLDEVAFIQNLVFYIEAAYKVADYGMWERGDKTNQGIPELNASSVGMAKAALEAIDELDLFGAHGGRKSVIHVLPDEVEHCQSILFSMLPRASTSKEIDAGLLSIISFPAFAVEDVNLVNVTKNEIISKLQGRYGCCRFLRDGYKTPREDPNRLHYDPAELKLFENIECEWPVFWTYFIIDGVFNGDAVQVQEYREALEGILIRGNNGVHLLPELYAIPPDKVDEEYKNPHTVDRVPLGKVPHLWGQSLYILSSLLAEGFLAAGEIDPLNRRFSTSVKPDVVVQVTVLAENNHIKELLRKHGVNVQSIADIHPIRVQPGRILSHIYAKLGRNKNMRLSGRPYRHIGVLGTSKLYVIRNQIFTFTPQFTDQHHFYLALDNEMIVEMLRIELAYLCTCWRMTGRPTLTFPITHTMLTNDGSDIHSAVLATIRKLEDGYFGGARVKLGSLSEFLTTSFYTYLTFLDPDCDEKLFDDASEGTFSPDSDSDLGGYLEDTYNQESQDELDQYINHLLQSTSSKCYLPPLCKKTEDNQVFSAIHSTRDILSVMAKAKGLEIPFVPMALPTKVLSAHRKSLNLVDSPQPLLKKAPEDDFQFPRDDHGDVDCEKLVEQLKDCSNLQDQADILYILYVIKGPSWDTNLSGQHGVTVHNLLSELYAKAGLNQEWGLIRYISGLLRKKVEVLAEACTDLLSHQKQLTVGLPPEPREKTISAPLPPEELTKLIYEASGQDISIAVLTQEIVVYLAMYVRAQPSLFVEMLRLRIGLIIQVMATELARSLNCSVRPIHSSTPSPAVSIHEVGHTGVTKTERSGINRLRSEMKQMTRRFSADEQLISVSQAASSSARSSKSARSSTPSSPTGTSSSDSGRQHMGWGERQGQWLRRRRLDGAINRVPVGFYQKVWKILQKCHGLSIDGYVLPSSTTQEMTPQEIKFAVHVESVLNRVPQPEYRQLLVEAIMVLTLLSDTEMNSIGGIIHVDQIVHMANQLFLQDQVSSGATDTLEKDQATGICHFFYDSAPSGAYGTMTYLTKAVASHLQELLPNSASGLLESEDRLEVGLSSAE

Target ORF information:

RefSeq Version	XM_005228394.3
Organism	Bos taurus(cattle)
Definition	Bos taurus phosphorylase kinase, alpha 2 (liver) (PHKA2), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005228394.3

ORF Insert Sequence:

ATGAGGAGCA GGAGCAATTC CGGGGTCCGC TTAGACGGAT ACGCGCGGCT GGTGCAGCAA 
ACAATCCTCT GTTACCAGAA TCCCATCACG GGGCTGATGT CAGCCAGTCA TGAGCAGAAG 
GACGCCTGGG TACGGGATAA CATCTACAGC ATCCTGGCCG TGTGGGGCCT GGGCATGGCC 
TACCGCAAGA ACGCCGACCG CGACGAGGAC AAGGCCAAGG CCTACGAGCT GGAGCAGAAT 
GTGGTGAAAC TGATGCGAGG TCTTCTCCAG TGCATGATGA GACAGGTGGA TAAAGTGGAG 
AGGTTCAAGC ACACTCAGAG TACCGAGGAC AGCCTGCACG CCAAGTACAA CACTGCCACC 
TGCAGCACTG TGGTGGGCGA CGACGAGTGG GGCCACCTCC AGGTGGATGC CACCTCCCTC 
TTCCTCCTGT TTCTGGCCCA GATGACAGCC TCCGGCTTAC GTATTATTTT CACTCTTGAC 
GAGGTGGCCT TCATACAGAA TCTTGTTTTT TACATAGAAG CTGCATATAA AGTTGCTGAT 
TATGGAATGT GGGAACGTGG AGATAAGACT AATCAGGGTA TTCCAGAATT GAATGCAAGC 
TCTGTAGGAA TGGCCAAGGC AGCCCTTGAA GCAATTGATG AACTGGACCT TTTTGGAGCC 
CATGGAGGTC GCAAGTCTGT GATCCACGTC CTGCCGGATG AGGTGGAACA CTGCCAGTCT 
ATTCTGTTCT CCATGCTGCC AAGAGCCTCG ACGTCAAAAG AGATTGATGC TGGGCTTCTT 
TCCATTATTT CTTTCCCGGC CTTCGCGGTG GAAGATGTGA ACCTTGTGAA TGTGACAAAA 
AATGAAATTA TTTCCAAGCT CCAGGGGCGT TATGGATGCT GTCGCTTCCT TCGAGATGGT 
TATAAAACCC CAAGAGAGGA CCCAAACCGA TTGCATTATG ACCCTGCTGA ACTCAAGCTC 
TTTGAAAACA TTGAATGTGA ATGGCCTGTG TTCTGGACTT ATTTCATAAT AGATGGAGTC 
TTCAATGGTG ATGCCGTTCA GGTCCAAGAA TACCGTGAAG CCCTGGAGGG GATACTGATC 
AGAGGCAATA ACGGGGTCCA CTTGTTGCCT GAACTCTACG CCATCCCACC TGACAAGGTG 
GATGAAGAGT ATAAGAATCC TCACACGGTG GATAGAGTTC CCCTGGGGAA GGTGCCGCAT 
CTGTGGGGTC AGTCCTTGTA CATCCTCAGC TCCCTGTTGG CAGAGGGATT CCTTGCTGCT 
GGTGAAATTG ATCCCTTGAA CAGAAGATTT TCCACTTCAG TCAAACCTGA TGTCGTCGTA 
CAAGTGACTG TTCTGGCAGA AAACAACCAC ATTAAGGAGC TTCTGAGGAA ACATGGGGTC 
AACGTCCAGA GTATCGCTGA CATTCATCCA ATCCGAGTCC AGCCAGGCCG CATCCTTAGT 
CACATATATG CCAAGCTTGG ACGGAATAAG AATATGAGAT TGAGTGGGCG ACCGTATCGG 
CACATTGGTG TCCTTGGGAC ATCGAAACTA TACGTGATTA GGAACCAGAT CTTTACTTTC 
ACGCCCCAGT TCACCGACCA GCATCACTTC TACCTGGCCC TGGACAATGA GATGATCGTA 
GAGATGCTGA GGATTGAGCT GGCCTACCTG TGCACCTGCT GGCGGATGAC GGGCAGACCC 
ACGCTCACCT TCCCCATCAC GCATACCATG CTCACAAATG ATGGCTCAGA TATTCATTCC 
GCTGTTCTTG CTACAATTCG CAAACTAGAG GATGGATATT TTGGAGGAGC TAGAGTAAAA 
TTAGGGAGTC TTTCGGAATT TCTCACCACC TCTTTCTACA CGTATCTGAC CTTCCTGGAT 
CCAGACTGTG ATGAGAAGTT GTTTGATGAT GCCAGTGAAG GGACCTTCAG TCCTGATAGT 
GATTCAGACC TGGGAGGATA TTTGGAAGAC ACCTATAATC AAGAAAGCCA GGACGAGCTT 
GACCAGTATA TCAACCACCT TCTGCAAAGC ACATCCTCGA AGTGCTATCT GCCACCTCTC 
TGTAAGAAGA CAGAGGACAA CCAGGTTTTC AGTGCTATCC ATTCCACTCG GGATATACTT 
TCTGTGATGG CAAAAGCAAA GGGCTTGGAA ATTCCATTTG TTCCCATGGC TTTGCCAACT 
AAAGTTCTAA GTGCCCACCG TAAATCGCTG AATCTCGTTG ATTCTCCTCA GCCACTCCTA 
AAAAAGGCCC CTGAAGATGA CTTCCAGTTT CCCAGAGATG ACCATGGCGA CGTGGACTGT 
GAGAAGCTGG TTGAGCAGTT GAAAGACTGT TCGAACCTAC AGGACCAAGC AGACATTCTG 
TACATTCTGT ATGTAATAAA GGGTCCCAGC TGGGACACAA ATCTCTCTGG ACAGCACGGG 
GTCACTGTTC ACAACCTCCT CAGTGAGCTC TATGCGAAGG CCGGCTTGAA CCAGGAGTGG 
GGCCTGATCC GCTACATCTC CGGCCTCCTC CGGAAGAAAG TGGAGGTCCT GGCTGAGGCG 
TGCACGGACC TGCTGTCACA CCAGAAGCAG CTCACAGTGG GCCTGCCCCC AGAACCCCGG 
GAGAAGACCA TCTCTGCGCC TCTTCCCCCC GAGGAGCTCA CGAAGCTCAT CTACGAAGCC 
AGCGGGCAGG ACATCAGCAT CGCCGTGCTC ACACAGGAGA TCGTGGTGTA CCTGGCGATG 
TACGTCAGGG CACAGCCCAG CCTCTTCGTG GAGATGCTCC GGCTCCGAAT TGGACTGATC 
ATTCAGGTGA TGGCCACGGA GCTGGCACGG AGCCTGAACT GTTCAGTGCG GCCCATTCAC 
TCCTCCACAC CTAGCCCTGC TGTCTCCATC CATGAGGTGG GCCACACTGG AGTCACCAAA 
ACCGAGAGGA GCGGCATCAA CAGGCTGAGG AGTGAGATGA AACAGATGAC TAGGCGGTTT 
AGTGCTGATG AACAGCTCAT TTCTGTGAGC CAGGCCGCAT CCAGCAGCGC GCGCTCCTCA 
AAGTCTGCGA GGTCCAGCAC CCCATCCTCA CCCACGGGCA CATCGTCATC AGACTCGGGC 
AGGCAGCACA TGGGCTGGGG GGAACGGCAG GGCCAGTGGC TGCGCAGGAG AAGGCTGGAC 
GGCGCCATCA ACAGGGTCCC CGTGGGATTC TACCAGAAGG TGTGGAAGAT CCTTCAGAAG 
TGCCATGGTC TGTCCATCGA CGGTTACGTG CTCCCGTCCT CGACCACGCA AGAGATGACC 
CCGCAGGAGA TCAAGTTTGC TGTCCACGTT GAGTCAGTGC TGAACCGAGT GCCACAACCC 
GAGTACCGGC AGCTGCTGGT GGAGGCCATC ATGGTGCTGA CGCTGCTCTC GGACACAGAG 
ATGAACAGCA TCGGGGGCAT CATCCATGTG GACCAGATCG TGCACATGGC CAACCAGCTG 
TTCCTACAAG ACCAGGTGTC AAGTGGAGCC ACGGACACGC TGGAGAAAGA CCAAGCCACA 
GGCATCTGCC ACTTCTTTTA CGACAGCGCT CCAAGCGGGG CTTATGGGAC GATGACCTAC 
CTAACGAAGG CGGTGGCTTC TCATTTGCAG GAACTGCTGC CCAATTCAGC ATCTGGTCTC 
CTGGAGTCTG AGGACAGATT GGAAGTGGGT CTCTCAAGTG CAGAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	PHKA2
Entrez Gene ID	515732
Full Name	phosphorylase kinase regulatory subunit alpha 2
General protein information	Preferred Names phosphorylase kinase regulatory subunit alpha 2 Names phosphorylase b kinase regulatory subunit alpha, liver isoform phosphorylase kinase, alpha 2 (liver) isoform 1 phosphorylase kinase, alpha 2 (liver) isoform 2
Gene Type	protein-coding
Organism	Bos taurus(cattle)

	PHKA2	XP_001084454.1
Rattus norvegicus (Norway rat)	Phka2	NP_001177923.1
Danio rerio (zebrafish)	LOC556199	XP_684044.4
Drosophila melanogaster (fruit fly)	CG7766	NP_001138180.1
Pan troglodytes (chimpanzee)	PHKA2	XP_003317431.1
Mus musculus (house mouse)	Phka2	NP_001171350.1
Homo sapiens (human)	PHKA2	NP_000283.1
Bos taurus (cattle)	PHKA2	NP_001178474.1
Canis lupus familiaris (dog)	PHKA2	XP_005641224.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP000708	XP_559348.4
Caenorhabditis elegans (roundworm)	C14B9.8	NP_498777.2
Gallus gallus (chicken)	PHKA2	XP_004934744.1
Xenopus tropicalis (tropical clawed frog)	phka2	XP_004911720.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

PHKA2 cDNA ORF clone, Bos taurus(cattle)

Gene

Clones

gRNAs

Related articles in PubMed

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