GREM2B cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	GREM2B
Entrez Gene ID	550399
Full Name	gremlin 2, DAN family BMP antagonist b
Synonyms	grem2,prdc,zgc:112207
General protein information	Preferred Names gremlin 2, DAN family BMP antagonist b Names gremlin-2 gremlin 2, cysteine knot superfamily protein related to DAN and cerberus homolog
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009306988.3	XP_009305263.1	gremlin-2 isoform X1
NM_001017704.1	NP_001017704.1	gremlin-2 precursor
XM_009306988.2	XP_009305263.1	gremlin-2 isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	GREM2	XP_005622630.1
Danio rerio (zebrafish)	grem2	NP_001017704.1
Pan troglodytes (chimpanzee)	GREM2	XP_525111.2
Rattus norvegicus (Norway rat)	Grem2	NP_001099444.1
Gallus gallus (chicken)	GREM2	XP_419552.3
Homo sapiens (human)	GREM2	NP_071914.3
	GREM2	XP_001095526.1
Mus musculus (house mouse)	Grem2	NP_035955.1

Gene Ontology
Bibliography

Related articles in PubMed

Genome-wide analysis of facial skeletal regionalization in zebrafish.
Askary A, Xu P, Barske L, Bay M, Bump P, Balczerski B, Bonaguidi MA, Crump JG
Development (Cambridge, England)144(16)2994-3005(2017 08)

Wdr68 Mediates Dorsal and Ventral Patterning Events for Craniofacial Development.
Alvarado E, Yousefelahiyeh M, Alvarado G, Shang R, Whitman T, Martinez A, Yu Y, Pham A, Bhandari A, Wang B, Nissen RM
PloS one11(11)e0166984(2016)

Functional modeling in zebrafish demonstrates that the atrial-fibrillation-associated gene GREM2 regulates cardiac laterality, cardiomyocyte differentiation and atrial rhythm.
M?ller II, Melville DB, Tanwar V, Rybski WM, Mukherjee A, Shoemaker MB, Wang WD, Schoenhard JA, Roden DM, Darbar D, Knapik EW, Hatzopoulos AK
Disease models & mechanisms6(2)332-41(2013 Mar)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Through regulation of bone morphogenetic protein signaling, GREM2 is required for cardiac laterality and atrial differentiation during embryonic development.
Title: Functional modeling in zebrafish demonstrates that the atrial-fibrillation-associated gene GREM2 regulates cardiac laterality, cardiomyocyte differentiation and atrial rhythm.

Gremlin 2 regulates distinct roles of BMP and Endothelin 1 signaling in dorsoventral patterning of the facial skeleton.
Title: Gremlin 2 regulates distinct roles of BMP and Endothelin 1 signaling in dorsoventral patterning of the facial skeleton.

Prdc is expressed in the developing eyes and the first two pharyngeal arches, in the outer layers of the optic cup, in the arch mesenchyme expands stepwise to the remaining posterior arches, and in the somites and the the swim bladder.
Title: Expression of the protein related to Dan and Cerberus gene--prdc--During eye, pharyngeal arch, somite, and swim bladder development in zebrafish.

The following GREM2B gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the GREM2B cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa03573	NM_001017704.1 Latest version!	Danio rerio gremlin 2, DAN family BMP antagonist b (grem2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa41760	XM_009306988.3 Latest version!	Danio rerio gremlin 2, DAN family BMP antagonist b (grem2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
View Old Accession Versions >>
ODa41760	XM_009306988.2	Danio rerio gremlin 2, DAN family BMP antagonist b (grem2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

GREM2B ( NM_001017704.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> NP_001017704.1 Danio rerio gremlin 2, DAN family BMP antagonist b (grem2b), mRNA.

Price & Availability↑

CloneID	ODa03573
Clone ID Related Accession (Same CDS sequence)	NM_001017704.1
Accession Version	NM_001017704.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 501bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-06-29
Organism	Danio rerio(zebrafish)
Product	gremlin-2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC093266.1. ##Evidence-Data-START## Transcript exon combination :: BC093266.1, EB835114.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGAGCAGTA AGGTGGCGCT GTGTCTGCTG TTGGCCGGCG CTCTGTGTGT GGTCTCCGCC 
GCCCGCAAGA ACCGCCCACA AAGCTCCGTC CCATCACCCT ACAAAACCAG CTCCAACACC 
TCTAACCGCC GCACGCGCAA ACAAGAAGTG CTCGCCTCCA GCCAGGAAGC GCTGGTGGTG 
ACCGAAAAGA AGTACCTGAA GAGCGACTGG TGTAAGACGC AGCCGTTGCG GCAGACGGTC 
AGCCAGGAGG GCTGCAGGAG CCGCACCGTC ATCAACCGCT TCTGCTACGG CCAATGCAAC 
TCCTTCTACA TCCCACGGCA CGTCAAGAAG GAGCAGGAGT CCTTCCAGTC CTGTGCGTTC 
TGCAGGCCGC AGCGCTTCAC CAGCCTCACT GTAGAGCTGG ACTGTTCAGA CCTGCAGCCG 
CCGGTCCGAT ACCGCAAGAT CCAGCGCATC AAGCAGTGCC GCTGCATGTC TGTGAGCGTG 
TCCGAGTCGG GGAAACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001017704.1
CDS	120..620
Translation	MSSKVALCLLLAGALCVVSAARKNRPQSSVPSPYKTSSNTSNRRTRKQEVLASSQEALVVTEKKYLKSDWCKTQPLRQTVSQEGCRSRTVINRFCYGQCNSFYIPRHVKKEQESFQSCAFCRPQRFTSLTVELDCSDLQPPVRYRKIQRIKQCRCMSVSVSESGKQ

Target ORF information:

RefSeq Version	NM_001017704.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio gremlin 2, DAN family BMP antagonist b (grem2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001017704.1

ORF Insert Sequence:

ATGAGCAGTA AGGTGGCGCT GTGTCTGCTG TTGGCCGGCG CTCTGTGTGT GGTCTCCGCC 
GCCCGCAAGA ACCGCCCACA AAGCTCCGTC CCATCACCCT ACAAAACCAG CTCCAACACC 
TCTAACCGCC GCACGCGCAA ACAAGAAGTG CTCGCCTCCA GCCAGGAAGC GCTGGTGGTG 
ACCGAAAAGA AGTACCTGAA GAGCGACTGG TGTAAGACGC AGCCGTTGCG GCAGACGGTC 
AGCCAGGAGG GCTGCAGGAG CCGCACCGTC ATCAACCGCT TCTGCTACGG CCAATGCAAC 
TCCTTCTACA TCCCACGGCA CGTCAAGAAG GAGCAGGAGT CCTTCCAGTC CTGTGCGTTC 
TGCAGGCCGC AGCGCTTCAC CAGCCTCACT GTAGAGCTGG ACTGTTCAGA CCTGCAGCCG 
CCGGTCCGAT ACCGCAAGAT CCAGCGCATC AAGCAGTGCC GCTGCATGTC TGTGAGCGTG 
TCCGAGTCGG GGAAACAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GREM2B ( XM_009306988.3 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009305263.1 Danio rerio gremlin 2, DAN family BMP antagonist b (grem2b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa41760
Clone ID Related Accession (Same CDS sequence)	XM_009306988.3 , XM_009306988.2
Accession Version	XM_009306988.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	gremlin-2 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306988.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGAGA CGCAGAAGAA GCAGCAGACA CACACACACA CACACAGATT TATAGAGTCC 
GTCAGAGATC AAACTCTCAG ACATACTGAA GACTCTCAGG GGCTGAACAT CCATGGGCTT 
CTTCATCATC ATCACCTTCA TCAGTGTCTG AGCACACACT CCAGCAGGAT GAGCAGTAAG 
GTGGCGCTGT GTCTGCTGTT GGCTGGCGCT CTGTGTGTGG TCTCCGCCGC CCGCAAGAAC 
CGCCCACAAA GCTCCGTCCC ATCACCCTAC AAAACCAGCT CCAACACCTC TGACCGCCGC 
ACGCGCAAAC AAGAAGTGCT CGCCTCCAGC CAGGAAGCGC TGGTGGTGAC CGAAAAGAAG 
TACCTGAAGA GCGACTGGTG TAAGACGCAG CCGTTGCGGC AGACGGTCAG CCAGGAGGGC 
TGCAGGAGCC GCACCGTCAT CAACCGCTTC TGCTACGGCC AATGCAACTC CTTCTACATC 
CCACGGCACG TCAAGAAGGA GCAGGAGTCC TTCCAGTCCT GTGCATTCTG CAGGCCGCAG 
CGCTTCACCA GCCTCACTGT AGAGCTGGAC TGTCCAGACC TGCAGCCGCC GGTCCGATAC 
CGCAAGATCC AGCGCATCAA GCAGTGCCGC TGCATGTCTG TGAGCGTGTC CGAGTCGGGG 
AAACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305263.1
CDS	175..843
Translation	MRETQKKQQTHTHTHRFIESVRDQTLRHTEDSQGLNIHGLLHHHHLHQCLSTHSSRMSSKVALCLLLAGALCVVSAARKNRPQSSVPSPYKTSSNTSDRRTRKQEVLASSQEALVVTEKKYLKSDWCKTQPLRQTVSQEGCRSRTVINRFCYGQCNSFYIPRHVKKEQESFQSCAFCRPQRFTSLTVELDCPDLQPPVRYRKIQRIKQCRCMSVSVSESGKQ

Target ORF information:

RefSeq Version	XM_009306988.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio gremlin 2, DAN family BMP antagonist b (grem2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306988.3

ORF Insert Sequence:

ATGCGAGAGA CGCAGAAGAA GCAGCAGACA CACACACACA CACACAGATT TATAGAGTCC 
GTCAGAGATC AAACTCTCAG ACATACTGAA GACTCTCAGG GGCTGAACAT CCATGGGCTT 
CTTCATCATC ATCACCTTCA TCAGTGTCTG AGCACACACT CCAGCAGGAT GAGCAGTAAG 
GTGGCGCTGT GTCTGCTGTT GGCTGGCGCT CTGTGTGTGG TCTCCGCCGC CCGCAAGAAC 
CGCCCACAAA GCTCCGTCCC ATCACCCTAC AAAACCAGCT CCAACACCTC TGACCGCCGC 
ACGCGCAAAC AAGAAGTGCT CGCCTCCAGC CAGGAAGCGC TGGTGGTGAC CGAAAAGAAG 
TACCTGAAGA GCGACTGGTG TAAGACGCAG CCGTTGCGGC AGACGGTCAG CCAGGAGGGC 
TGCAGGAGCC GCACCGTCAT CAACCGCTTC TGCTACGGCC AATGCAACTC CTTCTACATC 
CCACGGCACG TCAAGAAGGA GCAGGAGTCC TTCCAGTCCT GTGCATTCTG CAGGCCGCAG 
CGCTTCACCA GCCTCACTGT AGAGCTGGAC TGTCCAGACC TGCAGCCGCC GGTCCGATAC 
CGCAAGATCC AGCGCATCAA GCAGTGCCGC TGCATGTCTG TGAGCGTGTC CGAGTCGGGG 
AAACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

GREM2B ( XM_009306988.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_009305263.1 Danio rerio gremlin 2, DAN family BMP antagonist b (grem2b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa41760
Clone ID Related Accession (Same CDS sequence)	XM_009306988.3 , XM_009306988.2
Accession Version	XM_009306988.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 669bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	gremlin-2 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306988.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGAGAGA CGCAGAAGAA GCAGCAGACA CACACACACA CACACAGATT TATAGAGTCC 
GTCAGAGATC AAACTCTCAG ACATACTGAA GACTCTCAGG GGCTGAACAT CCATGGGCTT 
CTTCATCATC ATCACCTTCA TCAGTGTCTG AGCACACACT CCAGCAGGAT GAGCAGTAAG 
GTGGCGCTGT GTCTGCTGTT GGCTGGCGCT CTGTGTGTGG TCTCCGCCGC CCGCAAGAAC 
CGCCCACAAA GCTCCGTCCC ATCACCCTAC AAAACCAGCT CCAACACCTC TGACCGCCGC 
ACGCGCAAAC AAGAAGTGCT CGCCTCCAGC CAGGAAGCGC TGGTGGTGAC CGAAAAGAAG 
TACCTGAAGA GCGACTGGTG TAAGACGCAG CCGTTGCGGC AGACGGTCAG CCAGGAGGGC 
TGCAGGAGCC GCACCGTCAT CAACCGCTTC TGCTACGGCC AATGCAACTC CTTCTACATC 
CCACGGCACG TCAAGAAGGA GCAGGAGTCC TTCCAGTCCT GTGCATTCTG CAGGCCGCAG 
CGCTTCACCA GCCTCACTGT AGAGCTGGAC TGTCCAGACC TGCAGCCGCC GGTCCGATAC 
CGCAAGATCC AGCGCATCAA GCAGTGCCGC TGCATGTCTG TGAGCGTGTC CGAGTCGGGG 
AAACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009305263.1
CDS	83..751
Translation	MRETQKKQQTHTHTHRFIESVRDQTLRHTEDSQGLNIHGLLHHHHLHQCLSTHSSRMSSKVALCLLLAGALCVVSAARKNRPQSSVPSPYKTSSNTSDRRTRKQEVLASSQEALVVTEKKYLKSDWCKTQPLRQTVSQEGCRSRTVINRFCYGQCNSFYIPRHVKKEQESFQSCAFCRPQRFTSLTVELDCPDLQPPVRYRKIQRIKQCRCMSVSVSESGKQ

Target ORF information:

RefSeq Version	XM_009306988.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio gremlin 2, DAN family BMP antagonist b (grem2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306988.2

ORF Insert Sequence:

ATGCGAGAGA CGCAGAAGAA GCAGCAGACA CACACACACA CACACAGATT TATAGAGTCC 
GTCAGAGATC AAACTCTCAG ACATACTGAA GACTCTCAGG GGCTGAACAT CCATGGGCTT 
CTTCATCATC ATCACCTTCA TCAGTGTCTG AGCACACACT CCAGCAGGAT GAGCAGTAAG 
GTGGCGCTGT GTCTGCTGTT GGCTGGCGCT CTGTGTGTGG TCTCCGCCGC CCGCAAGAAC 
CGCCCACAAA GCTCCGTCCC ATCACCCTAC AAAACCAGCT CCAACACCTC TGACCGCCGC 
ACGCGCAAAC AAGAAGTGCT CGCCTCCAGC CAGGAAGCGC TGGTGGTGAC CGAAAAGAAG 
TACCTGAAGA GCGACTGGTG TAAGACGCAG CCGTTGCGGC AGACGGTCAG CCAGGAGGGC 
TGCAGGAGCC GCACCGTCAT CAACCGCTTC TGCTACGGCC AATGCAACTC CTTCTACATC 
CCACGGCACG TCAAGAAGGA GCAGGAGTCC TTCCAGTCCT GTGCATTCTG CAGGCCGCAG 
CGCTTCACCA GCCTCACTGT AGAGCTGGAC TGTCCAGACC TGCAGCCGCC GGTCCGATAC 
CGCAAGATCC AGCGCATCAA GCAGTGCCGC TGCATGTCTG TGAGCGTGTC CGAGTCGGGG 
AAACAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Genome-wide analysis of facial skeletal regionalization in zebrafish.
Development (Cambridge, England)144(16)2994-3005(2017 08)
Askary A,Xu P,Barske L,Bay M,Bump P,Balczerski B,Bonaguidi MA,Crump JG

Wdr68 Mediates Dorsal and Ventral Patterning Events for Craniofacial Development.
PloS one11(11)e0166984(2016)
Alvarado E,Yousefelahiyeh M,Alvarado G,Shang R,Whitman T,Martinez A,Yu Y,Pham A,Bhandari A,Wang B,Nissen RM

Functional modeling in zebrafish demonstrates that the atrial-fibrillation-associated gene GREM2 regulates cardiac laterality, cardiomyocyte differentiation and atrial rhythm.
Disease models & mechanisms6(2)332-41(2013 Mar)
M?ller II,Melville DB,Tanwar V,Rybski WM,Mukherjee A,Shoemaker MB,Wang WD,Schoenhard JA,Roden DM,Darbar D,Knapik EW,Hatzopoulos AK

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GREM2B cDNA ORF clone, Danio rerio(Zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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