numbl cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	numbl
Entrez Gene ID	497616
Full Name	numb homolog (Drosophila)-like
Synonyms	z-numb,zgc:123031
General protein information	Preferred Names numb homolog (Drosophila)-like Names numb-like protein
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001012375.3	NP_001012375.3	numb-like protein
XM_005173378.3	XP_005173435.1	numb-like protein isoform X1
XM_005173378.4	XP_005173435.1	numb-like protein isoform X1

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	NUMBL	XP_855314.2
Xenopus tropicalis (tropical clawed frog)	numbl	XP_002938862.2
Homo sapiens (human)	NUMBL	NP_004747.1
Mus musculus (house mouse)	Numbl	NP_035080.2
Pan troglodytes (chimpanzee)	NUMBL	XP_524270.4
Rattus norvegicus (Norway rat)	Numbl	NP_001029060.1
Danio rerio (zebrafish)	numbl	NP_001012375.3
Bos taurus (cattle)	NUMBL	NP_001070302.1

Gene Ontology
Bibliography

Related articles in PubMed

Transforming growth factor-?1 regulates the nascent hematopoietic stem cell niche by promoting gluconeogenesis.
Zhang CY, Yin HM, Wang H, Su D, Xia Y, Yan LF, Fang B, Liu W, Wang YM, Gu AH, Zhou Y
Leukemia(2017 Jun)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Lnx2 ubiquitin ligase is essential for exocrine cell differentiation in the early zebrafish pancreas.
Won M, Ro H, Dawid IB
Proceedings of the National Academy of Sciences of the United States of America112(40)12426-31(2015 Oct)

Evaluation and application of modularly assembled zinc-finger nucleases in zebrafish.
Zhu C, Smith T, McNulty J, Rayla AL, Lakshmanan A, Siekmann AF, Buffardi M, Meng X, Shin J, Padmanabhan A, Cifuentes D, Giraldez AJ, Look AT, Epstein JA, Lawson ND, Wolfe SA
Development (Cambridge, England)138(20)4555-64(2011 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

z-numb was essential for left-right field formation and maintenance in zebrafish
Title: Zebrafish Numb homologue: phylogenetic evolution and involvement in regulation of left-right asymmetry.

The following numbl gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the numbl cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa30780	NM_001012375.3 Latest version!	Danio rerio NUMB like endocytic adaptor protein (numbl), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
ODa20274	XM_005173378.4 Latest version!	Danio rerio numb homolog (Drosophila)-like (numbl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
View Old Accession Versions >>
ODa20274	XM_005173378.3	Danio rerio numb homolog (Drosophila)-like (numbl), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$342.30 $489.00
Hide Old Accession Versions >>

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

numbl ( NM_001012375.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001012375.3 Danio rerio NUMB like endocytic adaptor protein (numbl), mRNA.

Price & Availability↑

CloneID	ODa30780
Clone ID Related Accession (Same CDS sequence)	NM_001012375.3
Accession Version	NM_001012375.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1857bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-27
Organism	Danio rerio(zebrafish)
Product	numb-like protein
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CR318657.8 and CU019550.4. On or before May 19, 2009 this sequence version replaced NM_001128531.1, NM_001012375.2. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC107953.1, DQ022744.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGAATAAGC TGCGTCAGAG CCTGCGCAGG AAGAAGCCCA CCTACGTGCC AGAGGCCAGT 
CGACCACACC AGTGGCAGGC GGATGAAGAG GCTGTGCGAA AGGGCAAATG CAACTTTCCT 
GTCCGGTATC TCGGGCTGGT GGAGGTGGAT GAATCCAGAG GCATGCATGT GTGTGAAGAG 
GCAGTGAAAA AGCTAAAAGT GAGTGGGAAA AAGACAGTGA AGGCTGTGCT CTGGGTGTCT 
GCTGATGGCC TGAGAGTAGT GGACGACAAA ACCAAGGACC TGATTGTGGA TCAGACGATT 
GAAAAGGTGT CATTCTGTGC CCCGGACCGT AACTACGACA AGGCTTTCTC TTACATCTGC 
CGTGACGGAA CGACCCGCCG CTGGATGTGC CACTGCTTCA TGGCCCTGAA GGACTCGGGC 
GAGCGTCTCA GTCATGCAGT GGGATGTGCG TTTGCCGCCT GCCTGGAGCG GAAGCAGCGC 
AGAGAAAAGG AGTGTGGCGT CACAGCCTCT TTCGATGCCA GCAGAACATC ATTTGTGAGG 
GAAGGTTCAT TCCGCGTGTC CTCCGCCGCA CAACAGAGTG ACCGTGAGGA CATCATGAAG 
CAGATTCAAG ACAAGAAGAA AGAAATGTGT GGGATTTCTG CATTGCCGCC TGGAAATGCA 
TCTCCTCCAG AAGGGGCGGG GTCACCGGGT GAGAGGGCGG AGCCAGGTGG CCCTCACGCC 
ATTCCACGCA GACACGCCCC CATCGAGCAG CTGGTGCGAC AGGGATCCTT CAGAGGCTTT 
CCTCAACTCA GCCAGAAGAA CTCGCCGTTC AAACGACAGC TTTCTCTGCG TCTCAATGAT 
CTCCCGTCAA CCCTGCAGCG CAAGACCGAC TTCCAGACCA AGAACCCAGT TCCAGAGATG 
GATCTGACAG TGGCTGGAGA GGCGGACAGC ATTAACGCTC TGTGCAGCCA AATCAATAGC 
TCTTTCACCA AACCATCTGA AGACCTGTGT GCCAAAACCA CCAACAACTG CTTACCCATC 
AGCTGCGCTC CACCTGCCAT CCCTCCTCCA CCTGCCCCTC CTCAAGCTAC AAATTTATGG 
GTCCAAATAG AGCCCCCGGT CCAGTCTTCT CCACCCATTC CTCAGGGTGG CCACAAACGA 
ACCCCGTCCG AAGCAGAACG ATGGCTGGAG GAAGTTGCTA AAGCGGTGAA ATCCCAACAG 
CAGACTCCAC CTACTTTACC ACCACCACCC ACTCAGCAGC CTCCACCGGT GCCCACCATG 
CCCATTGCAC CCGGTTCCCT CCCAACATCC ATGCAGCCAT TTCCGATGGC TTTTGACGTC 
ACTCCTGCTC CCGTTAGTAT GTTCACCCAA CAGCCGCTCC AACCGGCGTT TGTACCTATG 
CAGCCGTACA TGCCCACTCT AGCCAACAGC ATGACCTATC CCAATGCAAG TGTACCCGTG 
GTGGGCATCA CACCCTCACA GATGGTGGCG AATGTATTTT GCAGTGCAGC CGGTACAGGA 
GGTGGAGTGG CAATTGGGGT CGGGATGGGG CCTAAGACGG GTACGCTTGG CGGGAGCCAA 
CATTCAGCAT TTCCCACCCT TCCTGGAGGT CTTCCAACCA CCACGTTCGC TTCTCCTCCA 
ACAATCAATG GCCACCCACA AAACTCCTTT CCATCAACGA CCACCAGTGT TACGCAAAAC 
GGTACTGCCG TTAAAGGAGG TTCTGGTTGG CCCATGGAGA ATCCGAATCC CACAATCAAC 
TCCAAGGAAG CCGAGCATTT CGATGCAAAG TGGGCCGCCC TGGAGTCCAA ATCACAACCC 
AAGGCGCAAA ACCCATTTTC CAACGACTTG CAGAAGACTT TTGAGATTGA ACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001012375.3
CDS	223..2079
Translation	MNKLRQSLRRKKPTYVPEASRPHQWQADEEAVRKGKCNFPVRYLGLVEVDESRGMHVCEEAVKKLKVSGKKTVKAVLWVSADGLRVVDDKTKDLIVDQTIEKVSFCAPDRNYDKAFSYICRDGTTRRWMCHCFMALKDSGERLSHAVGCAFAACLERKQRREKECGVTASFDASRTSFVREGSFRVSSAAQQSDREDIMKQIQDKKKEMCGISALPPGNASPPEGAGSPGERAEPGGPHAIPRRHAPIEQLVRQGSFRGFPQLSQKNSPFKRQLSLRLNDLPSTLQRKTDFQTKNPVPEMDLTVAGEADSINALCSQINSSFTKPSEDLCAKTTNNCLPISCAPPAIPPPPAPPQATNLWVQIEPPVQSSPPIPQGGHKRTPSEAERWLEEVAKAVKSQQQTPPTLPPPPTQQPPPVPTMPIAPGSLPTSMQPFPMAFDVTPAPVSMFTQQPLQPAFVPMQPYMPTLANSMTYPNASVPVVGITPSQMVANVFCSAAGTGGGVAIGVGMGPKTGTLGGSQHSAFPTLPGGLPTTTFASPPTINGHPQNSFPSTTTSVTQNGTAVKGGSGWPMENPNPTINSKEAEHFDAKWAALESKSQPKAQNPFSNDLQKTFEIEL

Target ORF information:

RefSeq Version	NM_001012375.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio NUMB like endocytic adaptor protein (numbl), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001012375.3

ORF Insert Sequence:

ATGAATAAGC TGCGTCAGAG CCTGCGCAGG AAGAAGCCCA CCTACGTGCC AGAGGCCAGT 
CGACCACACC AGTGGCAGGC GGATGAAGAG GCTGTGCGAA AGGGCAAATG CAACTTTCCT 
GTCCGGTATC TCGGGCTGGT GGAGGTGGAT GAATCCAGAG GCATGCATGT GTGTGAAGAG 
GCAGTGAAAA AGCTAAAAGT GAGTGGGAAA AAGACAGTGA AGGCTGTGCT CTGGGTGTCT 
GCTGATGGCC TGAGAGTAGT GGACGACAAA ACCAAGGACC TGATTGTGGA TCAGACGATT 
GAAAAGGTGT CATTCTGTGC CCCGGACCGT AACTACGACA AGGCTTTCTC TTACATCTGC 
CGTGACGGAA CGACCCGCCG CTGGATGTGC CACTGCTTCA TGGCCCTGAA GGACTCGGGC 
GAGCGTCTCA GTCATGCAGT GGGATGTGCG TTTGCCGCCT GCCTGGAGCG GAAGCAGCGC 
AGAGAAAAGG AGTGTGGCGT CACAGCCTCT TTCGATGCCA GCAGAACATC ATTTGTGAGG 
GAAGGTTCAT TCCGCGTGTC CTCCGCCGCA CAACAGAGTG ACCGTGAGGA CATCATGAAG 
CAGATTCAAG ACAAGAAGAA AGAAATGTGT GGGATTTCTG CATTGCCGCC TGGAAATGCA 
TCTCCTCCAG AAGGGGCGGG GTCACCGGGT GAGAGGGCGG AGCCAGGTGG CCCTCACGCC 
ATTCCACGCA GACACGCCCC CATCGAGCAG CTGGTGCGAC AGGGATCCTT CAGAGGCTTT 
CCTCAACTCA GCCAGAAGAA CTCGCCGTTC AAACGACAGC TTTCTCTGCG TCTCAATGAT 
CTCCCGTCAA CCCTGCAGCG CAAGACCGAC TTCCAGACCA AGAACCCAGT TCCAGAGATG 
GATCTGACAG TGGCTGGAGA GGCGGACAGC ATTAACGCTC TGTGCAGCCA AATCAATAGC 
TCTTTCACCA AACCATCTGA AGACCTGTGT GCCAAAACCA CCAACAACTG CTTACCCATC 
AGCTGCGCTC CACCTGCCAT CCCTCCTCCA CCTGCCCCTC CTCAAGCTAC AAATTTATGG 
GTCCAAATAG AGCCCCCGGT CCAGTCTTCT CCACCCATTC CTCAGGGTGG CCACAAACGA 
ACCCCGTCCG AAGCAGAACG ATGGCTGGAG GAAGTTGCTA AAGCGGTGAA ATCCCAACAG 
CAGACTCCAC CTACTTTACC ACCACCACCC ACTCAGCAGC CTCCACCGGT GCCCACCATG 
CCCATTGCAC CCGGTTCCCT CCCAACATCC ATGCAGCCAT TTCCGATGGC TTTTGACGTC 
ACTCCTGCTC CCGTTAGTAT GTTCACCCAA CAGCCGCTCC AACCGGCGTT TGTACCTATG 
CAGCCGTACA TGCCCACTCT AGCCAACAGC ATGACCTATC CCAATGCAAG TGTACCCGTG 
GTGGGCATCA CACCCTCACA GATGGTGGCG AATGTATTTT GCAGTGCAGC CGGTACAGGA 
GGTGGAGTGG CAATTGGGGT CGGGATGGGG CCTAAGACGG GTACGCTTGG CGGGAGCCAA 
CATTCAGCAT TTCCCACCCT TCCTGGAGGT CTTCCAACCA CCACGTTCGC TTCTCCTCCA 
ACAATCAATG GCCACCCACA AAACTCCTTT CCATCAACGA CCACCAGTGT TACGCAAAAC 
GGTACTGCCG TTAAAGGAGG TTCTGGTTGG CCCATGGAGA ATCCGAATCC CACAATCAAC 
TCCAAGGAAG CCGAGCATTT CGATGCAAAG TGGGCCGCCC TGGAGTCCAA ATCACAACCC 
AAGGCGCAAA ACCCATTTTC CAACGACTTG CAGAAGACTT TTGAGATTGA ACTATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

numbl ( XM_005173378.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005173435.1 Danio rerio numb homolog (Drosophila)-like (numbl), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa20274
Clone ID Related Accession (Same CDS sequence)	XM_005173378.3 , XM_005173378.4
Accession Version	XM_005173378.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	numb-like protein isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005173378.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTTCA GCAGTGACAT GGATGAGACT GAAGATGGGA GCACCAGAGA GTTTGAGAGC 
GTGGAGATGA ATAAGCTGCG TCAGAGCCTG CGCAGGAAGA AGCCCACCTA CGTGCCAGAG 
GCCAGTCGAC CACACCAGTG GCAGGCGGAT GAAGAGGCTG TGCGAAAGGG CAAATGCAAC 
TTTCCTGTCC GGTATCTCGG GCTGGTGGAG GTGGATGAAT CCAGAGGCAT GCATGTGTGT 
GAAGAGGCAG TGAAAAAGCT AAAAGTGAGT GGGAAAAAGA CAGTGAAGGC TGTGCTCTGG 
GTGTCTGCTG ATGGCCTGAG AGTAGTGGAC GACAAAACCA AGGACCTGAT TGTGGATCAG 
ACGATTGAAA AGGTGTCATT CTGTGCCCCG GACCGTAACT ACGACAAGGC TTTCTCTTAC 
ATCTGCCGTG ACGGAACGAC CCGCCGCTGG ATGTGCCACT GCTTCATGGC CCTGAAGGAC 
TCGGGCGAGC GTCTCAGTCA TGCAGTGGGA TGTGCGTTTG CCGCCTGCCT GGAGCGGAAG 
CAGCGCAGAG AAAAGGAGTG TGGCGTCACA GCCTCTTTCG ATGCCAGCAG AACATCATTT 
GTGAGGGAAG GTTCATTCCG CGTGTCCTCC GCCGCACAAC AGAGTGACCG TGAGGACATC 
ATGAAGCAGA TTCAAGACAA GAAGAAAGAA ATGTGTGGGA TTTCTGCATT GCCGCCTGGA 
AATGCATCTC CTCCAGAAGG GGCGGGGTCA CCGGGTGAGA GGGCGGAGCC AGGTGGCCCT 
CACGCCATTC CACGCAGACA CGCCCCCATC GAGCAGCTGG TGCGACAGGG ATCCTTCAGA 
GGCTTTCCTC AACTCAGCCA GAAGAACTCG CCGTTCAAAC GACAGCTTTC TCTGCGTCTC 
AATGATCTCC CGTCAACCCT GCAGCGCAAG ACCGACTTCC AGACCAAGAA CCCAGTTCCA 
GAGATGGATC TGACAGTGGC TGGAGAGGCG GACAGCATTA ACGCTCTGTG CAGCCAAATC 
AATAGCTCTT TCACCAAACC ATCTGAAGAC CTGTGTGCCA AAACCACCAA CAACTGCTTA 
CCCATCAGCT GCGCTCCACC TGCCATCCCT CCTCCACCTG CCCCTCCTCA AGCTACAAAT 
TTATGGGTCC AAATAGAGCC CCCGGTCCAG TCTTCTCCAC CCATTCCTCA GGGTGGCCAC 
AAACGAACCC CGTCCGAAGC AGAACGATGG CTGGAGGAAG TTGCTAAAGC GGTGAAATCC 
CAACAGCAGA CTCCACCTAC TTTACCACCA CCACCCACTC AGCAGCCTCC ACCGGTGCCC 
ACCATGCCCA TTGCACCCGG TTCCCTCCCA ACATCCATGC AGCCATTTCC GATGGCTTTT 
GACGTCACTC CTGCTCCCGT TAGTATGTTC ACCCAACAGC CGCTCCAACC GGCGTTTGTA 
CCTATGCAGC CGTACATGCC CACTCTAGCC AACAGCATGA CCTATCCCAA TGCAAGTGTA 
CCCGTGGTGG GCATCACACC CTCACAGATG GTGGCGAATG TATTTTGCAG TGCAGCCGGT 
ACAGGAGGTG GAGTGGCAAT TGGGGTCGGG ATGGGGCCTA AGACGGGTAC GCTTGGCGGG 
AGCCAACATT CAGCATTTCC CACCCTTCCT GGAGGTCTTC CAACCACCAC GTTCGCTTCT 
CCTCCAACAA TCAATGGCCA CCCACAAAAC TCCTTTCCAT CAACGACCAC CAGTGTTACG 
CAAAACGGTA CTGCCGTTAA AGGAGGTTCT GGTTGGCCCA TGGAGAATCC GAATCCCACA 
ATCAACTCCA AGGAAGCCGA GCATTTCGAT GCAAAGTGGG CCGCCCTGGA GTCCAAATCA 
CAACCCAAGG CGCAAAACCC ATTTTCCAAC GACTTGCAGA AGACTTTTGA GATTGAACTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173435.1
CDS	1536..3458
Translation	MSFSSDMDETEDGSTREFESVEMNKLRQSLRRKKPTYVPEASRPHQWQADEEAVRKGKCNFPVRYLGLVEVDESRGMHVCEEAVKKLKVSGKKTVKAVLWVSADGLRVVDDKTKDLIVDQTIEKVSFCAPDRNYDKAFSYICRDGTTRRWMCHCFMALKDSGERLSHAVGCAFAACLERKQRREKECGVTASFDASRTSFVREGSFRVSSAAQQSDREDIMKQIQDKKKEMCGISALPPGNASPPEGAGSPGERAEPGGPHAIPRRHAPIEQLVRQGSFRGFPQLSQKNSPFKRQLSLRLNDLPSTLQRKTDFQTKNPVPEMDLTVAGEADSINALCSQINSSFTKPSEDLCAKTTNNCLPISCAPPAIPPPPAPPQATNLWVQIEPPVQSSPPIPQGGHKRTPSEAERWLEEVAKAVKSQQQTPPTLPPPPTQQPPPVPTMPIAPGSLPTSMQPFPMAFDVTPAPVSMFTQQPLQPAFVPMQPYMPTLANSMTYPNASVPVVGITPSQMVANVFCSAAGTGGGVAIGVGMGPKTGTLGGSQHSAFPTLPGGLPTTTFASPPTINGHPQNSFPSTTTSVTQNGTAVKGGSGWPMENPNPTINSKEAEHFDAKWAALESKSQPKAQNPFSNDLQKTFEIEL

Target ORF information:

RefSeq Version	XM_005173378.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio numb homolog (Drosophila)-like (numbl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173378.3

ORF Insert Sequence:

ATGAGTTTCA GCAGTGACAT GGATGAGACT GAAGATGGGA GCACCAGAGA GTTTGAGAGC 
GTGGAGATGA ATAAGCTGCG TCAGAGCCTG CGCAGGAAGA AGCCCACCTA CGTGCCAGAG 
GCCAGTCGAC CACACCAGTG GCAGGCGGAT GAAGAGGCTG TGCGAAAGGG CAAATGCAAC 
TTTCCTGTCC GGTATCTCGG GCTGGTGGAG GTGGATGAAT CCAGAGGCAT GCATGTGTGT 
GAAGAGGCAG TGAAAAAGCT AAAAGTGAGT GGGAAAAAGA CAGTGAAGGC TGTGCTCTGG 
GTGTCTGCTG ATGGCCTGAG AGTAGTGGAC GACAAAACCA AGGACCTGAT TGTGGATCAG 
ACGATTGAAA AGGTGTCATT CTGTGCCCCG GACCGTAACT ACGACAAGGC TTTCTCTTAC 
ATCTGCCGTG ACGGAACGAC CCGCCGCTGG ATGTGCCACT GCTTCATGGC CCTGAAGGAC 
TCGGGCGAGC GTCTCAGTCA TGCAGTGGGA TGTGCGTTTG CCGCCTGCCT GGAGCGGAAG 
CAGCGCAGAG AAAAGGAGTG TGGCGTCACA GCCTCTTTCG ATGCCAGCAG AACATCATTT 
GTGAGGGAAG GTTCATTCCG CGTGTCCTCC GCCGCACAAC AGAGTGACCG TGAGGACATC 
ATGAAGCAGA TTCAAGACAA GAAGAAAGAA ATGTGTGGGA TTTCTGCATT GCCGCCTGGA 
AATGCATCTC CTCCAGAAGG GGCGGGGTCA CCGGGTGAGA GGGCGGAGCC AGGTGGCCCT 
CACGCCATTC CACGCAGACA CGCCCCCATC GAGCAGCTGG TGCGACAGGG ATCCTTCAGA 
GGCTTTCCTC AACTCAGCCA GAAGAACTCG CCGTTCAAAC GACAGCTTTC TCTGCGTCTC 
AATGATCTCC CGTCAACCCT GCAGCGCAAG ACCGACTTCC AGACCAAGAA CCCAGTTCCA 
GAGATGGATC TGACAGTGGC TGGAGAGGCG GACAGCATTA ACGCTCTGTG CAGCCAAATC 
AATAGCTCTT TCACCAAACC ATCTGAAGAC CTGTGTGCCA AAACCACCAA CAACTGCTTA 
CCCATCAGCT GCGCTCCACC TGCCATCCCT CCTCCACCTG CCCCTCCTCA AGCTACAAAT 
TTATGGGTCC AAATAGAGCC CCCGGTCCAG TCTTCTCCAC CCATTCCTCA GGGTGGCCAC 
AAACGAACCC CGTCCGAAGC AGAACGATGG CTGGAGGAAG TTGCTAAAGC GGTGAAATCC 
CAACAGCAGA CTCCACCTAC TTTACCACCA CCACCCACTC AGCAGCCTCC ACCGGTGCCC 
ACCATGCCCA TTGCACCCGG TTCCCTCCCA ACATCCATGC AGCCATTTCC GATGGCTTTT 
GACGTCACTC CTGCTCCCGT TAGTATGTTC ACCCAACAGC CGCTCCAACC GGCGTTTGTA 
CCTATGCAGC CGTACATGCC CACTCTAGCC AACAGCATGA CCTATCCCAA TGCAAGTGTA 
CCCGTGGTGG GCATCACACC CTCACAGATG GTGGCGAATG TATTTTGCAG TGCAGCCGGT 
ACAGGAGGTG GAGTGGCAAT TGGGGTCGGG ATGGGGCCTA AGACGGGTAC GCTTGGCGGG 
AGCCAACATT CAGCATTTCC CACCCTTCCT GGAGGTCTTC CAACCACCAC GTTCGCTTCT 
CCTCCAACAA TCAATGGCCA CCCACAAAAC TCCTTTCCAT CAACGACCAC CAGTGTTACG 
CAAAACGGTA CTGCCGTTAA AGGAGGTTCT GGTTGGCCCA TGGAGAATCC GAATCCCACA 
ATCAACTCCA AGGAAGCCGA GCATTTCGAT GCAAAGTGGG CCGCCCTGGA GTCCAAATCA 
CAACCCAAGG CGCAAAACCC ATTTTCCAAC GACTTGCAGA AGACTTTTGA GATTGAACTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

numbl ( XM_005173378.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005173435.1 Danio rerio numb homolog (Drosophila)-like (numbl), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa20274
Clone ID Related Accession (Same CDS sequence)	XM_005173378.3 , XM_005173378.4
Accession Version	XM_005173378.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1923bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	numb-like protein isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by mRNA evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005173378.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGTTTCA GCAGTGACAT GGATGAGACT GAAGATGGGA GCACCAGAGA GTTTGAGAGC 
GTGGAGATGA ATAAGCTGCG TCAGAGCCTG CGCAGGAAGA AGCCCACCTA CGTGCCAGAG 
GCCAGTCGAC CACACCAGTG GCAGGCGGAT GAAGAGGCTG TGCGAAAGGG CAAATGCAAC 
TTTCCTGTCC GGTATCTCGG GCTGGTGGAG GTGGATGAAT CCAGAGGCAT GCATGTGTGT 
GAAGAGGCAG TGAAAAAGCT AAAAGTGAGT GGGAAAAAGA CAGTGAAGGC TGTGCTCTGG 
GTGTCTGCTG ATGGCCTGAG AGTAGTGGAC GACAAAACCA AGGACCTGAT TGTGGATCAG 
ACGATTGAAA AGGTGTCATT CTGTGCCCCG GACCGTAACT ACGACAAGGC TTTCTCTTAC 
ATCTGCCGTG ACGGAACGAC CCGCCGCTGG ATGTGCCACT GCTTCATGGC CCTGAAGGAC 
TCGGGCGAGC GTCTCAGTCA TGCAGTGGGA TGTGCGTTTG CCGCCTGCCT GGAGCGGAAG 
CAGCGCAGAG AAAAGGAGTG TGGCGTCACA GCCTCTTTCG ATGCCAGCAG AACATCATTT 
GTGAGGGAAG GTTCATTCCG CGTGTCCTCC GCCGCACAAC AGAGTGACCG TGAGGACATC 
ATGAAGCAGA TTCAAGACAA GAAGAAAGAA ATGTGTGGGA TTTCTGCATT GCCGCCTGGA 
AATGCATCTC CTCCAGAAGG GGCGGGGTCA CCGGGTGAGA GGGCGGAGCC AGGTGGCCCT 
CACGCCATTC CACGCAGACA CGCCCCCATC GAGCAGCTGG TGCGACAGGG ATCCTTCAGA 
GGCTTTCCTC AACTCAGCCA GAAGAACTCG CCGTTCAAAC GACAGCTTTC TCTGCGTCTC 
AATGATCTCC CGTCAACCCT GCAGCGCAAG ACCGACTTCC AGACCAAGAA CCCAGTTCCA 
GAGATGGATC TGACAGTGGC TGGAGAGGCG GACAGCATTA ACGCTCTGTG CAGCCAAATC 
AATAGCTCTT TCACCAAACC ATCTGAAGAC CTGTGTGCCA AAACCACCAA CAACTGCTTA 
CCCATCAGCT GCGCTCCACC TGCCATCCCT CCTCCACCTG CCCCTCCTCA AGCTACAAAT 
TTATGGGTCC AAATAGAGCC CCCGGTCCAG TCTTCTCCAC CCATTCCTCA GGGTGGCCAC 
AAACGAACCC CGTCCGAAGC AGAACGATGG CTGGAGGAAG TTGCTAAAGC GGTGAAATCC 
CAACAGCAGA CTCCACCTAC TTTACCACCA CCACCCACTC AGCAGCCTCC ACCGGTGCCC 
ACCATGCCCA TTGCACCCGG TTCCCTCCCA ACATCCATGC AGCCATTTCC GATGGCTTTT 
GACGTCACTC CTGCTCCCGT TAGTATGTTC ACCCAACAGC CGCTCCAACC GGCGTTTGTA 
CCTATGCAGC CGTACATGCC CACTCTAGCC AACAGCATGA CCTATCCCAA TGCAAGTGTA 
CCCGTGGTGG GCATCACACC CTCACAGATG GTGGCGAATG TATTTTGCAG TGCAGCCGGT 
ACAGGAGGTG GAGTGGCAAT TGGGGTCGGG ATGGGGCCTA AGACGGGTAC GCTTGGCGGG 
AGCCAACATT CAGCATTTCC CACCCTTCCT GGAGGTCTTC CAACCACCAC GTTCGCTTCT 
CCTCCAACAA TCAATGGCCA CCCACAAAAC TCCTTTCCAT CAACGACCAC CAGTGTTACG 
CAAAACGGTA CTGCCGTTAA AGGAGGTTCT GGTTGGCCCA TGGAGAATCC GAATCCCACA 
ATCAACTCCA AGGAAGCCGA GCATTTCGAT GCAAAGTGGG CCGCCCTGGA GTCCAAATCA 
CAACCCAAGG CGCAAAACCC ATTTTCCAAC GACTTGCAGA AGACTTTTGA GATTGAACTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005173435.1
CDS	1021..2943
Translation	MSFSSDMDETEDGSTREFESVEMNKLRQSLRRKKPTYVPEASRPHQWQADEEAVRKGKCNFPVRYLGLVEVDESRGMHVCEEAVKKLKVSGKKTVKAVLWVSADGLRVVDDKTKDLIVDQTIEKVSFCAPDRNYDKAFSYICRDGTTRRWMCHCFMALKDSGERLSHAVGCAFAACLERKQRREKECGVTASFDASRTSFVREGSFRVSSAAQQSDREDIMKQIQDKKKEMCGISALPPGNASPPEGAGSPGERAEPGGPHAIPRRHAPIEQLVRQGSFRGFPQLSQKNSPFKRQLSLRLNDLPSTLQRKTDFQTKNPVPEMDLTVAGEADSINALCSQINSSFTKPSEDLCAKTTNNCLPISCAPPAIPPPPAPPQATNLWVQIEPPVQSSPPIPQGGHKRTPSEAERWLEEVAKAVKSQQQTPPTLPPPPTQQPPPVPTMPIAPGSLPTSMQPFPMAFDVTPAPVSMFTQQPLQPAFVPMQPYMPTLANSMTYPNASVPVVGITPSQMVANVFCSAAGTGGGVAIGVGMGPKTGTLGGSQHSAFPTLPGGLPTTTFASPPTINGHPQNSFPSTTTSVTQNGTAVKGGSGWPMENPNPTINSKEAEHFDAKWAALESKSQPKAQNPFSNDLQKTFEIEL

Target ORF information:

RefSeq Version	XM_005173378.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio numb homolog (Drosophila)-like (numbl), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005173378.4

ORF Insert Sequence:

ATGAGTTTCA GCAGTGACAT GGATGAGACT GAAGATGGGA GCACCAGAGA GTTTGAGAGC 
GTGGAGATGA ATAAGCTGCG TCAGAGCCTG CGCAGGAAGA AGCCCACCTA CGTGCCAGAG 
GCCAGTCGAC CACACCAGTG GCAGGCGGAT GAAGAGGCTG TGCGAAAGGG CAAATGCAAC 
TTTCCTGTCC GGTATCTCGG GCTGGTGGAG GTGGATGAAT CCAGAGGCAT GCATGTGTGT 
GAAGAGGCAG TGAAAAAGCT AAAAGTGAGT GGGAAAAAGA CAGTGAAGGC TGTGCTCTGG 
GTGTCTGCTG ATGGCCTGAG AGTAGTGGAC GACAAAACCA AGGACCTGAT TGTGGATCAG 
ACGATTGAAA AGGTGTCATT CTGTGCCCCG GACCGTAACT ACGACAAGGC TTTCTCTTAC 
ATCTGCCGTG ACGGAACGAC CCGCCGCTGG ATGTGCCACT GCTTCATGGC CCTGAAGGAC 
TCGGGCGAGC GTCTCAGTCA TGCAGTGGGA TGTGCGTTTG CCGCCTGCCT GGAGCGGAAG 
CAGCGCAGAG AAAAGGAGTG TGGCGTCACA GCCTCTTTCG ATGCCAGCAG AACATCATTT 
GTGAGGGAAG GTTCATTCCG CGTGTCCTCC GCCGCACAAC AGAGTGACCG TGAGGACATC 
ATGAAGCAGA TTCAAGACAA GAAGAAAGAA ATGTGTGGGA TTTCTGCATT GCCGCCTGGA 
AATGCATCTC CTCCAGAAGG GGCGGGGTCA CCGGGTGAGA GGGCGGAGCC AGGTGGCCCT 
CACGCCATTC CACGCAGACA CGCCCCCATC GAGCAGCTGG TGCGACAGGG ATCCTTCAGA 
GGCTTTCCTC AACTCAGCCA GAAGAACTCG CCGTTCAAAC GACAGCTTTC TCTGCGTCTC 
AATGATCTCC CGTCAACCCT GCAGCGCAAG ACCGACTTCC AGACCAAGAA CCCAGTTCCA 
GAGATGGATC TGACAGTGGC TGGAGAGGCG GACAGCATTA ACGCTCTGTG CAGCCAAATC 
AATAGCTCTT TCACCAAACC ATCTGAAGAC CTGTGTGCCA AAACCACCAA CAACTGCTTA 
CCCATCAGCT GCGCTCCACC TGCCATCCCT CCTCCACCTG CCCCTCCTCA AGCTACAAAT 
TTATGGGTCC AAATAGAGCC CCCGGTCCAG TCTTCTCCAC CCATTCCTCA GGGTGGCCAC 
AAACGAACCC CGTCCGAAGC AGAACGATGG CTGGAGGAAG TTGCTAAAGC GGTGAAATCC 
CAACAGCAGA CTCCACCTAC TTTACCACCA CCACCCACTC AGCAGCCTCC ACCGGTGCCC 
ACCATGCCCA TTGCACCCGG TTCCCTCCCA ACATCCATGC AGCCATTTCC GATGGCTTTT 
GACGTCACTC CTGCTCCCGT TAGTATGTTC ACCCAACAGC CGCTCCAACC GGCGTTTGTA 
CCTATGCAGC CGTACATGCC CACTCTAGCC AACAGCATGA CCTATCCCAA TGCAAGTGTA 
CCCGTGGTGG GCATCACACC CTCACAGATG GTGGCGAATG TATTTTGCAG TGCAGCCGGT 
ACAGGAGGTG GAGTGGCAAT TGGGGTCGGG ATGGGGCCTA AGACGGGTAC GCTTGGCGGG 
AGCCAACATT CAGCATTTCC CACCCTTCCT GGAGGTCTTC CAACCACCAC GTTCGCTTCT 
CCTCCAACAA TCAATGGCCA CCCACAAAAC TCCTTTCCAT CAACGACCAC CAGTGTTACG 
CAAAACGGTA CTGCCGTTAA AGGAGGTTCT GGTTGGCCCA TGGAGAATCC GAATCCCACA 
ATCAACTCCA AGGAAGCCGA GCATTTCGAT GCAAAGTGGG CCGCCCTGGA GTCCAAATCA 
CAACCCAAGG CGCAAAACCC ATTTTCCAAC GACTTGCAGA AGACTTTTGA GATTGAACTA 
TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Transforming growth factor-?1 regulates the nascent hematopoietic stem cell niche by promoting gluconeogenesis.
Leukemia(2017 Jun)
Zhang CY,Yin HM,Wang H,Su D,Xia Y,Yan LF,Fang B,Liu W,Wang YM,Gu AH,Zhou Y

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Lnx2 ubiquitin ligase is essential for exocrine cell differentiation in the early zebrafish pancreas.
Proceedings of the National Academy of Sciences of the United States of America112(40)12426-31(2015 Oct)
Won M,Ro H,Dawid IB

Evaluation and application of modularly assembled zinc-finger nucleases in zebrafish.
Development (Cambridge, England)138(20)4555-64(2011 Oct)
Zhu C,Smith T,McNulty J,Rayla AL,Lakshmanan A,Siekmann AF,Buffardi M,Meng X,Shin J,Padmanabhan A,Cifuentes D,Giraldez AJ,Look AT,Epstein JA,Lawson ND,Wolfe SA

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numbl cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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