ank1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ank1a
Entrez Gene ID	449796
Full Name	ankyrin 1, erythrocytic a
Synonyms	ank1,wu:fa09h06,zgc:101835
General protein information	Preferred Names ankyrin 1, erythrocytic a Names ankyrin 1, erythrocytic a
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_021476105.1	XP_021331780.1	ankyrin 1, erythrocytic a isoform X7
XM_021476111.1	XP_021331786.1	ankyrin 1, erythrocytic a isoform X13
XM_021476117.1	XP_021331792.1	ankyrin 1, erythrocytic a isoform X18
XM_021476119.1	XP_021331794.1	ankyrin 1, erythrocytic a isoform X21
XM_021476121.1	XP_021331796.1	ankyrin 1, erythrocytic a isoform X25
XM_009302009.2	XP_009300284.1	ankyrin 1, erythrocytic a isoform X3
XM_017356075.1	XP_017211564.1	ankyrin 1, erythrocytic a isoform X9
XM_005165649.3	XP_005165706.1	ankyrin 1, erythrocytic a isoform X15
NM_001005969.2	NP_001005969.2	ankyrin 1, erythrocytic a isoform 2 precursor
XM_009302007.2	XP_009300282.1	ankyrin 1, erythrocytic a isoform X1
XM_009302010.2	XP_009300285.1	ankyrin 1, erythrocytic a isoform X4
XM_009302014.2	XP_009300289.1	ankyrin 1, erythrocytic a isoform X7
XM_021476099.1	XP_021331774.1	ankyrin 1, erythrocytic a isoform X1
XM_021476102.1	XP_021331777.1	ankyrin 1, erythrocytic a isoform X4
XM_021476109.1	XP_021331784.1	ankyrin 1, erythrocytic a isoform X11
XM_021476115.1	XP_021331790.1	ankyrin 1, erythrocytic a isoform X17
XM_021476116.1	XP_021331791.1	ankyrin 1, erythrocytic a isoform X18
XM_005165648.4	XP_005165705.1	ankyrin 1, erythrocytic a isoform X22
XM_009302011.2	XP_009300286.1	ankyrin 1, erythrocytic a isoform X5
XM_009302016.2	XP_009300291.1	ankyrin 1, erythrocytic a isoform X11
XM_009302017.2	XP_009300292.1	ankyrin 1, erythrocytic a isoform X12
XM_021476100.1	XP_021331775.1	ankyrin 1, erythrocytic a isoform X2
XM_021476103.1	XP_021331778.1	ankyrin 1, erythrocytic a isoform X5
XM_021476104.1	XP_021331779.1	ankyrin 1, erythrocytic a isoform X6
XM_021476107.1	XP_021331782.1	ankyrin 1, erythrocytic a isoform X9
XM_005165649.4	XP_005165706.1	ankyrin 1, erythrocytic a isoform X23
XM_009302008.2	XP_009300283.1	ankyrin 1, erythrocytic a isoform X2
XM_017356074.1	XP_017211563.1	ankyrin 1, erythrocytic a isoform X8
XM_005165647.3	XP_005165704.1	ankyrin 1, erythrocytic a isoform X13
XM_021476101.1	XP_021331776.1	ankyrin 1, erythrocytic a isoform X3
XM_021476112.1	XP_021331787.1	ankyrin 1, erythrocytic a isoform X14
XM_021476113.1	XP_021331788.1	ankyrin 1, erythrocytic a isoform X15
XM_021476114.1	XP_021331789.1	ankyrin 1, erythrocytic a isoform X16
XM_021476118.1	XP_021331793.1	ankyrin 1, erythrocytic a isoform X19
XM_021476120.1	XP_021331795.1	ankyrin 1, erythrocytic a isoform X24
XM_021476122.1	XP_021331797.1	ankyrin 1, erythrocytic a isoform X26
NM_001327890.1	NP_001314819.1	ankyrin 1, erythrocytic a isoform 1
XM_009302013.2	XP_009300288.1	ankyrin 1, erythrocytic a isoform X6
XM_009302015.2	XP_009300290.1	ankyrin 1, erythrocytic a isoform X10
XM_005165648.3	XP_005165705.1	ankyrin 1, erythrocytic a isoform X14
XM_005165650.3	XP_005165707.1	ankyrin 1, erythrocytic a isoform X16
XM_021476106.1	XP_021331781.1	ankyrin 1, erythrocytic a isoform X8
XM_021476108.1	XP_021331783.1	ankyrin 1, erythrocytic a isoform X10
XM_021476110.1	XP_021331785.1	ankyrin 1, erythrocytic a isoform X12
XM_005165647.4	XP_005165704.1	ankyrin 1, erythrocytic a isoform X20

Pathways from BioSystems

Homologies

Xenopus tropicalis (tropical clawed frog)	ank1	NP_001027488.1
Danio rerio (zebrafish)	ank1	NP_001005969.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Assignment of Functional Relevance to Genes at Type 2 Diabetes-Associated Loci Through Investigation of ?-Cell Mass Deficits.
O'Hare EA, Yerges-Armstrong LM, Perry JA, Shuldiner AR, Zaghloul NA
Molecular endocrinology (Baltimore, Md.)30(4)429-45(2016 Apr)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following ank1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ank1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa11251	XM_005165647.4 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X21, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa11252	XM_005165648.4 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X23, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa11253	XM_005165649.4 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X24, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa11254	XM_005165650.3 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa60548	NM_001005969.2 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant 2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
ODa142610	XM_021476119.1 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X22, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
ODa142611	XM_021476120.1 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X25, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
ODa142612	XM_021476121.1 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X26, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	9-11	$104.30 $149.00
ODa142609	XM_021476118.1 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X20, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa142602	XM_021476110.1 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa142603	XM_021476111.1 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa142604	XM_021476112.1 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa142605	XM_021476113.1 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa142606	XM_021476114.1 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X16, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa142607	XM_021476115.1 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X17, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa142608	XM_021476116.1 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X18, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa142608	XM_021476117.1 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X19, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa59074	XM_017356074.1 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa59075	XM_017356075.1 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$1021.30 $1459.00
ODa41278	NM_001327890.1 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant 1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa41279	XM_009302007.2 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa41280	XM_009302008.2 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa41281	XM_009302009.2 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa41282	XM_009302010.2 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa41283	XM_009302011.2 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa41285	XM_009302013.2 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa41286	XM_009302014.2 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa41287	XM_009302015.2 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$1021.30 $1459.00
ODa41288	XM_009302016.2 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa41289	XM_009302017.2 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa142591	XM_021476099.1 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa142592	XM_021476100.1 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa142593	XM_021476101.1 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa142594	XM_021476102.1 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa142595	XM_021476103.1 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa142596	XM_021476104.1 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa142597	XM_021476105.1 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa142598	XM_021476106.1 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa142599	XM_021476107.1 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa142600	XM_021476108.1 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa142601	XM_021476109.1 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
ODa142613	XM_021476122.1 Latest version!	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X27, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	29-31	$1161.30 $1659.00
View Old Accession Versions >>
ODa11253	XM_005165649.3	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X15, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa11252	XM_005165648.3	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
ODa11251	XM_005165647.3	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00
Hide Old Accession Versions >>

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2-4 Clone	40% OFF
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ank1a ( XM_005165647.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165704.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X13, mRNA.

Price & Availability↑

CloneID	ODa11251
Clone ID Related Accession (Same CDS sequence)	XM_005165647.3 , XM_005165647.4
Accession Version	XM_005165647.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ankyrin 1, erythrocytic a isoform X13
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165647.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCTC TGGTCACGGA GCTGTTCTTC AGCTTTATTC TGCTCGCCTT TATGGTGATC 
AGCTGCCAGA ATGTTCTGCA TATCGCCAGC AGCTCAGTGC GCTCTGTGCT GGCCTACATA 
CACACTCAGC TGGACCGAGA GCTGGGCGAG AATGAAGGAG TGGCAGACGA GGAGGAAAAC 
GTCACCACTC GGGTCGTGCG CCGCAGGGTT ATACTGAAGG GTGATGAGGC TGAGGATCTT 
CCAGGAGAGC AAGTGAGCGA GGAGCAATTC ACAGACGAGC ATGGCAACAT CGTCACCAAA 
AAGATTGTGA GGAAAGTGGT CCGGAGAGGA AAAGGGGAGG AAGGGGGTCA GGAGCTGATC 
ATTGAAGGTC TGCAGCAGGA TATCAGTGAG CCTGACTTTG ATGGAGAACA GTTCATGAGC 
TATGCCGTCC TGGGACGTGA CAGCAAGCCG GATGTTGTGG ATGTGAAGAG AGGAGGTGCT 
CAGATAGTCA AATGTGCGAG TCTGCGGAGA GTAAAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165704.1
CDS	295..816
Translation	MWALVTELFFSFILLAFMVISCQNVLHIASSSVRSVLAYIHTQLDRELGENEGVADEEENVTTRVVRRRVILKGDEAEDLPGEQVSEEQFTDEHGNIVTKKIVRKVVRRGKGEEGGQELIIEGLQQDISEPDFDGEQFMSYAVLGRDSKPDVVDVKRGGAQIVKCASLRRVKQ

Target ORF information:

RefSeq Version	XM_005165647.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165647.3

ORF Insert Sequence:

ATGTGGGCTC TGGTCACGGA GCTGTTCTTC AGCTTTATTC TGCTCGCCTT TATGGTGATC 
AGCTGCCAGA ATGTTCTGCA TATCGCCAGC AGCTCAGTGC GCTCTGTGCT GGCCTACATA 
CACACTCAGC TGGACCGAGA GCTGGGCGAG AATGAAGGAG TGGCAGACGA GGAGGAAAAC 
GTCACCACTC GGGTCGTGCG CCGCAGGGTT ATACTGAAGG GTGATGAGGC TGAGGATCTT 
CCAGGAGAGC AAGTGAGCGA GGAGCAATTC ACAGACGAGC ATGGCAACAT CGTCACCAAA 
AAGATTGTGA GGAAAGTGGT CCGGAGAGGA AAAGGGGAGG AAGGGGGTCA GGAGCTGATC 
ATTGAAGGTC TGCAGCAGGA TATCAGTGAG CCTGACTTTG ATGGAGAACA GTTCATGAGC 
TATGCCGTCC TGGGACGTGA CAGCAAGCCG GATGTTGTGG ATGTGAAGAG AGGAGGTGCT 
CAGATAGTCA AATGTGCGAG TCTGCGGAGA GTAAAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_005165647.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165704.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X21, mRNA.

Price & Availability↑

CloneID	ODa11251
Clone ID Related Accession (Same CDS sequence)	XM_005165647.3 , XM_005165647.4
Accession Version	XM_005165647.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin 1, erythrocytic a isoform X20
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165647.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCTC TGGTCACGGA GCTGTTCTTC AGCTTTATTC TGCTCGCCTT TATGGTGATC 
AGCTGCCAGA ATGTTCTGCA TATCGCCAGC AGCTCAGTGC GCTCTGTGCT GGCCTACATA 
CACACTCAGC TGGACCGAGA GCTGGGCGAG AATGAAGGAG TGGCAGACGA GGAGGAAAAC 
GTCACCACTC GGGTCGTGCG CCGCAGGGTT ATACTGAAGG GTGATGAGGC TGAGGATCTT 
CCAGGAGAGC AAGTGAGCGA GGAGCAATTC ACAGACGAGC ATGGCAACAT CGTCACCAAA 
AAGATTGTGA GGAAAGTGGT CCGGAGAGGA AAAGGGGAGG AAGGGGGTCA GGAGCTGATC 
ATTGAAGGTC TGCAGCAGGA TATCAGTGAG CCTGACTTTG ATGGAGAACA GTTCATGAGC 
TATGCCGTCC TGGGACGTGA CAGCAAGCCG GATGTTGTGG ATGTGAAGAG AGGAGGTGCT 
CAGATAGTCA AATGTGCGAG TCTGCGGAGA GTAAAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165704.1
CDS	225..746
Translation	MWALVTELFFSFILLAFMVISCQNVLHIASSSVRSVLAYIHTQLDRELGENEGVADEEENVTTRVVRRRVILKGDEAEDLPGEQVSEEQFTDEHGNIVTKKIVRKVVRRGKGEEGGQELIIEGLQQDISEPDFDGEQFMSYAVLGRDSKPDVVDVKRGGAQIVKCASLRRVKQ

Target ORF information:

RefSeq Version	XM_005165647.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X21, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165647.4

ORF Insert Sequence:

ATGTGGGCTC TGGTCACGGA GCTGTTCTTC AGCTTTATTC TGCTCGCCTT TATGGTGATC 
AGCTGCCAGA ATGTTCTGCA TATCGCCAGC AGCTCAGTGC GCTCTGTGCT GGCCTACATA 
CACACTCAGC TGGACCGAGA GCTGGGCGAG AATGAAGGAG TGGCAGACGA GGAGGAAAAC 
GTCACCACTC GGGTCGTGCG CCGCAGGGTT ATACTGAAGG GTGATGAGGC TGAGGATCTT 
CCAGGAGAGC AAGTGAGCGA GGAGCAATTC ACAGACGAGC ATGGCAACAT CGTCACCAAA 
AAGATTGTGA GGAAAGTGGT CCGGAGAGGA AAAGGGGAGG AAGGGGGTCA GGAGCTGATC 
ATTGAAGGTC TGCAGCAGGA TATCAGTGAG CCTGACTTTG ATGGAGAACA GTTCATGAGC 
TATGCCGTCC TGGGACGTGA CAGCAAGCCG GATGTTGTGG ATGTGAAGAG AGGAGGTGCT 
CAGATAGTCA AATGTGCGAG TCTGCGGAGA GTAAAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_005165648.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165705.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X23, mRNA.

Price & Availability↑

CloneID	ODa11252
Clone ID Related Accession (Same CDS sequence)	XM_005165648.4 , XM_005165648.3
Accession Version	XM_005165648.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin 1, erythrocytic a isoform X22
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165648.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCTC TGGTCACGGA GCTGTTCTTC AGCTTTATTC TGCTCGCCTT TATGGTGATC 
AGCTGCCAGA ATGTTCTGCA TATCGCCAGC AGCTCAGTGC GCTCTGTGCT GGCCTACATA 
CACACTCAGC TGGACCGAGA GCTGGGCGAG AATGAAGGAG TGGCAGACGA GGAGGAAAAC 
GTCACCACTC GGGTCGTGCG CCGCAGGGTT ATACTGAAGG GTGATGAGGC TGAGGATCTT 
CCAGGAGAGC AAGTGAGCGA GGAGCAATTC ACAGACGAGC ATGGCAACAT CGTCACCAAA 
AAGATTGTGA GGAAAGTGGT CCGGAGAGGA AAAGGGGAGG AAGGGGGTCA GGAGCTGATC 
ATTGAAGGTC TGCAGCAGGA TATCAGTGAG CCTGACTTTG ATGGAGAACA GTTCATGAGC 
TATGCCGTCC TGGGACGTGA CAGCAAGGAC TCGACTCCTT CACCCAAACC ATCCTTCACT 
GACTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165705.1
CDS	225..713
Translation	MWALVTELFFSFILLAFMVISCQNVLHIASSSVRSVLAYIHTQLDRELGENEGVADEEENVTTRVVRRRVILKGDEAEDLPGEQVSEEQFTDEHGNIVTKKIVRKVVRRGKGEEGGQELIIEGLQQDISEPDFDGEQFMSYAVLGRDSKDSTPSPKPSFTDS

Target ORF information:

RefSeq Version	XM_005165648.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X23, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165648.4

ORF Insert Sequence:

ATGTGGGCTC TGGTCACGGA GCTGTTCTTC AGCTTTATTC TGCTCGCCTT TATGGTGATC 
AGCTGCCAGA ATGTTCTGCA TATCGCCAGC AGCTCAGTGC GCTCTGTGCT GGCCTACATA 
CACACTCAGC TGGACCGAGA GCTGGGCGAG AATGAAGGAG TGGCAGACGA GGAGGAAAAC 
GTCACCACTC GGGTCGTGCG CCGCAGGGTT ATACTGAAGG GTGATGAGGC TGAGGATCTT 
CCAGGAGAGC AAGTGAGCGA GGAGCAATTC ACAGACGAGC ATGGCAACAT CGTCACCAAA 
AAGATTGTGA GGAAAGTGGT CCGGAGAGGA AAAGGGGAGG AAGGGGGTCA GGAGCTGATC 
ATTGAAGGTC TGCAGCAGGA TATCAGTGAG CCTGACTTTG ATGGAGAACA GTTCATGAGC 
TATGCCGTCC TGGGACGTGA CAGCAAGGAC TCGACTCCTT CACCCAAACC ATCCTTCACT 
GACTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_005165648.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165705.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X14, mRNA.

Price & Availability↑

CloneID	ODa11252
Clone ID Related Accession (Same CDS sequence)	XM_005165648.4 , XM_005165648.3
Accession Version	XM_005165648.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 489bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ankyrin 1, erythrocytic a isoform X14
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165648.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCTC TGGTCACGGA GCTGTTCTTC AGCTTTATTC TGCTCGCCTT TATGGTGATC 
AGCTGCCAGA ATGTTCTGCA TATCGCCAGC AGCTCAGTGC GCTCTGTGCT GGCCTACATA 
CACACTCAGC TGGACCGAGA GCTGGGCGAG AATGAAGGAG TGGCAGACGA GGAGGAAAAC 
GTCACCACTC GGGTCGTGCG CCGCAGGGTT ATACTGAAGG GTGATGAGGC TGAGGATCTT 
CCAGGAGAGC AAGTGAGCGA GGAGCAATTC ACAGACGAGC ATGGCAACAT CGTCACCAAA 
AAGATTGTGA GGAAAGTGGT CCGGAGAGGA AAAGGGGAGG AAGGGGGTCA GGAGCTGATC 
ATTGAAGGTC TGCAGCAGGA TATCAGTGAG CCTGACTTTG ATGGAGAACA GTTCATGAGC 
TATGCCGTCC TGGGACGTGA CAGCAAGGAC TCGACTCCTT CACCCAAACC ATCCTTCACT 
GACTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165705.1
CDS	291..779
Translation	MWALVTELFFSFILLAFMVISCQNVLHIASSSVRSVLAYIHTQLDRELGENEGVADEEENVTTRVVRRRVILKGDEAEDLPGEQVSEEQFTDEHGNIVTKKIVRKVVRRGKGEEGGQELIIEGLQQDISEPDFDGEQFMSYAVLGRDSKDSTPSPKPSFTDS

Target ORF information:

RefSeq Version	XM_005165648.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165648.3

ORF Insert Sequence:

ATGTGGGCTC TGGTCACGGA GCTGTTCTTC AGCTTTATTC TGCTCGCCTT TATGGTGATC 
AGCTGCCAGA ATGTTCTGCA TATCGCCAGC AGCTCAGTGC GCTCTGTGCT GGCCTACATA 
CACACTCAGC TGGACCGAGA GCTGGGCGAG AATGAAGGAG TGGCAGACGA GGAGGAAAAC 
GTCACCACTC GGGTCGTGCG CCGCAGGGTT ATACTGAAGG GTGATGAGGC TGAGGATCTT 
CCAGGAGAGC AAGTGAGCGA GGAGCAATTC ACAGACGAGC ATGGCAACAT CGTCACCAAA 
AAGATTGTGA GGAAAGTGGT CCGGAGAGGA AAAGGGGAGG AAGGGGGTCA GGAGCTGATC 
ATTGAAGGTC TGCAGCAGGA TATCAGTGAG CCTGACTTTG ATGGAGAACA GTTCATGAGC 
TATGCCGTCC TGGGACGTGA CAGCAAGGAC TCGACTCCTT CACCCAAACC ATCCTTCACT 
GACTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_005165649.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165706.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X15, mRNA.

Price & Availability↑

CloneID	ODa11253
Clone ID Related Accession (Same CDS sequence)	XM_005165649.3 , XM_005165649.4
Accession Version	XM_005165649.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ankyrin 1, erythrocytic a isoform X15
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165649.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCTC TGGTCACGGA GCTGTTCTTC AGCTTTATTC TGCTCGCCTT TATGGTGATC 
AGCTGCCAGA ATGTTCTGCA TATCGCCAGC AGCTCAGTGC GCTCTGTGCT GGCCTACATA 
CACACTCAGC TGGACCGAGA GCTGGGCGAG AATGAAGGAG TGGCAGACGA GGAGGAAAAC 
GTCACCACTC GGGTCGTGCG CCGCAGGGTT ATACTGAAGG GTGATGAGGC TGAGGATCTT 
CCAGGAGAGC AAGTGAGCGA GGAGCAATTC ACAGACGAGC ATGGCAACAT CGTCACCAAA 
AAGATTGTGA GGAAAGTGGT CCGGAGAGGA AAAGGGGAGG AAGGGGGTCA GGAGCTGATC 
ATTGAAGGTC TGCAGCAGGA TATCAGTGAG CCTGACTTTG ATGGAGAACA GTTCATGAGC 
TATGCCGTCC TGGGACGTGA CAGCAAGATC TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165706.1
CDS	296..751
Translation	MWALVTELFFSFILLAFMVISCQNVLHIASSSVRSVLAYIHTQLDRELGENEGVADEEENVTTRVVRRRVILKGDEAEDLPGEQVSEEQFTDEHGNIVTKKIVRKVVRRGKGEEGGQELIIEGLQQDISEPDFDGEQFMSYAVLGRDSKIL

Target ORF information:

RefSeq Version	XM_005165649.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165649.3

ORF Insert Sequence:

ATGTGGGCTC TGGTCACGGA GCTGTTCTTC AGCTTTATTC TGCTCGCCTT TATGGTGATC 
AGCTGCCAGA ATGTTCTGCA TATCGCCAGC AGCTCAGTGC GCTCTGTGCT GGCCTACATA 
CACACTCAGC TGGACCGAGA GCTGGGCGAG AATGAAGGAG TGGCAGACGA GGAGGAAAAC 
GTCACCACTC GGGTCGTGCG CCGCAGGGTT ATACTGAAGG GTGATGAGGC TGAGGATCTT 
CCAGGAGAGC AAGTGAGCGA GGAGCAATTC ACAGACGAGC ATGGCAACAT CGTCACCAAA 
AAGATTGTGA GGAAAGTGGT CCGGAGAGGA AAAGGGGAGG AAGGGGGTCA GGAGCTGATC 
ATTGAAGGTC TGCAGCAGGA TATCAGTGAG CCTGACTTTG ATGGAGAACA GTTCATGAGC 
TATGCCGTCC TGGGACGTGA CAGCAAGATC TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_005165649.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165706.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X24, mRNA.

Price & Availability↑

CloneID	ODa11253
Clone ID Related Accession (Same CDS sequence)	XM_005165649.3 , XM_005165649.4
Accession Version	XM_005165649.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 456bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin 1, erythrocytic a isoform X23
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165649.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCTC TGGTCACGGA GCTGTTCTTC AGCTTTATTC TGCTCGCCTT TATGGTGATC 
AGCTGCCAGA ATGTTCTGCA TATCGCCAGC AGCTCAGTGC GCTCTGTGCT GGCCTACATA 
CACACTCAGC TGGACCGAGA GCTGGGCGAG AATGAAGGAG TGGCAGACGA GGAGGAAAAC 
GTCACCACTC GGGTCGTGCG CCGCAGGGTT ATACTGAAGG GTGATGAGGC TGAGGATCTT 
CCAGGAGAGC AAGTGAGCGA GGAGCAATTC ACAGACGAGC ATGGCAACAT CGTCACCAAA 
AAGATTGTGA GGAAAGTGGT CCGGAGAGGA AAAGGGGAGG AAGGGGGTCA GGAGCTGATC 
ATTGAAGGTC TGCAGCAGGA TATCAGTGAG CCTGACTTTG ATGGAGAACA GTTCATGAGC 
TATGCCGTCC TGGGACGTGA CAGCAAGATC TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165706.1
CDS	223..678
Translation	MWALVTELFFSFILLAFMVISCQNVLHIASSSVRSVLAYIHTQLDRELGENEGVADEEENVTTRVVRRRVILKGDEAEDLPGEQVSEEQFTDEHGNIVTKKIVRKVVRRGKGEEGGQELIIEGLQQDISEPDFDGEQFMSYAVLGRDSKIL

Target ORF information:

RefSeq Version	XM_005165649.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X24, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165649.4

ORF Insert Sequence:

ATGTGGGCTC TGGTCACGGA GCTGTTCTTC AGCTTTATTC TGCTCGCCTT TATGGTGATC 
AGCTGCCAGA ATGTTCTGCA TATCGCCAGC AGCTCAGTGC GCTCTGTGCT GGCCTACATA 
CACACTCAGC TGGACCGAGA GCTGGGCGAG AATGAAGGAG TGGCAGACGA GGAGGAAAAC 
GTCACCACTC GGGTCGTGCG CCGCAGGGTT ATACTGAAGG GTGATGAGGC TGAGGATCTT 
CCAGGAGAGC AAGTGAGCGA GGAGCAATTC ACAGACGAGC ATGGCAACAT CGTCACCAAA 
AAGATTGTGA GGAAAGTGGT CCGGAGAGGA AAAGGGGAGG AAGGGGGTCA GGAGCTGATC 
ATTGAAGGTC TGCAGCAGGA TATCAGTGAG CCTGACTTTG ATGGAGAACA GTTCATGAGC 
TATGCCGTCC TGGGACGTGA CAGCAAGATC TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_005165650.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165707.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X16, mRNA.

Price & Availability↑

CloneID	ODa11254
Clone ID Related Accession (Same CDS sequence)	XM_005165650.3
Accession Version	XM_005165650.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 330bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ankyrin 1, erythrocytic a isoform X16
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165650.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAACCA ACACCATCCT TTTTGGAGCT GACGCTGCCA CCAGTTTCCT CCGCGCAGCT 
CGCTCAGGTA ATCTGGAAAA GGCTCTAGAC CACATCAAGA ACGGCATCGA CATCAACACA 
GCCAATCAGA ATGGACTCAA CGGGCTGCAT TTGGCCTCCA AGGAAGGCCA TGTTAAAATG 
GTGCTGGAGC TGCTTCACCA TGGTATTGTA CTTGAGACGA CAACGAAGAA AGGGAACACT 
GCTCTTCATA TAGCGGCGTT GGCTGGCCAG GAGCAGGTGG TCACTGAACT GGTCAACTAT 
GGAGCAAACG TCAACGCTCA GTCTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165707.1
CDS	346..675
Translation	MPTNTILFGADAATSFLRAARSGNLEKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQ

Target ORF information:

RefSeq Version	XM_005165650.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165650.3

ORF Insert Sequence:

ATGCCAACCA ACACCATCCT TTTTGGAGCT GACGCTGCCA CCAGTTTCCT CCGCGCAGCT 
CGCTCAGGTA ATCTGGAAAA GGCTCTAGAC CACATCAAGA ACGGCATCGA CATCAACACA 
GCCAATCAGA ATGGACTCAA CGGGCTGCAT TTGGCCTCCA AGGAAGGCCA TGTTAAAATG 
GTGCTGGAGC TGCTTCACCA TGGTATTGTA CTTGAGACGA CAACGAAGAA AGGGAACACT 
GCTCTTCATA TAGCGGCGTT GGCTGGCCAG GAGCAGGTGG TCACTGAACT GGTCAACTAT 
GGAGCAAACG TCAACGCTCA GTCTCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( NM_001005969.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001005969.2 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant 2, mRNA.

Price & Availability↑

CloneID	ODa60548
Clone ID Related Accession (Same CDS sequence)	NM_001005969.2
Accession Version	NM_001005969.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 459bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-27
Organism	Danio rerio(zebrafish)
Product	ankyrin 1, erythrocytic a isoform 2 precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CT033825.21. On May 27, 2016 this sequence version replaced NM_001005969.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC083296.1, EH610961.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA3505370, SAMEA3505380 [ECO:0000348] ##Evidence-Data-END##

ATGTGGGCTC TGGTCACGGA GCTGTTCTTC AGCTTTATTC TGCTCGCCTT TATGGTGATC 
AGCTGCCAGA ATGTTCTGCA TATCGCCAGC AGCTCAGTGC GCTCTGTGCT GGCCTACATA 
CACACTCAGC TGGACCGAGA GCTGGGCGAG AATGAAGGAG TGGCAGACGA GGAGGAAAAC 
GTCACCACTC GGGTCGTGCG CCGCAGGGTT ATACTGAAGG GTGATGAGGC TGAGGATCTT 
CCAGGAGAGC AAGTGAGCGA GGAGCAATTC ACAGACGAGC ATGGCAACAT CGTCACCAAA 
AAGATTGTGA GGAAAGTGGT CCGGAGAGGA AAAGGGGAGG AAGGGGGTCA GGAGCTGATC 
ATTGAAGGTC TGCAGCAGGA TATCAGTGAG CCTGACTTTG ATGGAGAACA GTTCATGAGC 
TATGCCGTCC TGGGACGTGA CAGCAAGGTG GGCTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001005969.2
CDS	185..643
Translation	MWALVTELFFSFILLAFMVISCQNVLHIASSSVRSVLAYIHTQLDRELGENEGVADEEENVTTRVVRRRVILKGDEAEDLPGEQVSEEQFTDEHGNIVTKKIVRKVVRRGKGEEGGQELIIEGLQQDISEPDFDGEQFMSYAVLGRDSKVGF

Target ORF information:

RefSeq Version	NM_001005969.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant 2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001005969.2

ORF Insert Sequence:

ATGTGGGCTC TGGTCACGGA GCTGTTCTTC AGCTTTATTC TGCTCGCCTT TATGGTGATC 
AGCTGCCAGA ATGTTCTGCA TATCGCCAGC AGCTCAGTGC GCTCTGTGCT GGCCTACATA 
CACACTCAGC TGGACCGAGA GCTGGGCGAG AATGAAGGAG TGGCAGACGA GGAGGAAAAC 
GTCACCACTC GGGTCGTGCG CCGCAGGGTT ATACTGAAGG GTGATGAGGC TGAGGATCTT 
CCAGGAGAGC AAGTGAGCGA GGAGCAATTC ACAGACGAGC ATGGCAACAT CGTCACCAAA 
AAGATTGTGA GGAAAGTGGT CCGGAGAGGA AAAGGGGAGG AAGGGGGTCA GGAGCTGATC 
ATTGAAGGTC TGCAGCAGGA TATCAGTGAG CCTGACTTTG ATGGAGAACA GTTCATGAGC 
TATGCCGTCC TGGGACGTGA CAGCAAGGTG GGCTTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_021476119.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331794.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X22, mRNA.

Price & Availability↑

CloneID	ODa142610
Clone ID Related Accession (Same CDS sequence)	XM_021476119.1
Accession Version	XM_021476119.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 522bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin 1, erythrocytic a isoform X21
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331794.1
CDS	242..763
Translation	MAQAAKHLRKNKDLEALAEQERKEKEEEKSKKRSRSREKKRKAHVVHRWLIDQDNSVSSELPDGQGVWHYDETADAATSFLRAARSGNLEKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQ

Target ORF information:

RefSeq Version	XM_021476119.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X22, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476119.1

ORF Insert Sequence:

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_021476120.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331795.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X25, mRNA.

Price & Availability↑

CloneID	ODa142611
Clone ID Related Accession (Same CDS sequence)	XM_021476120.1
Accession Version	XM_021476120.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 378bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin 1, erythrocytic a isoform X24
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTGGGCTC TGGTCACGGA GCTGTTCTTC AGCTTTATTC TGCTCGCCTT TATGGTGATC 
AGCTGCCAGA ATGTTCTGCA TATCGCCAGC AGCTCAGTGC GCTCTGTGCT GGCCTACATA 
CACACTCAGC TGGACCGAGA GCTGGGCGAG AATGAAGGAG TGGCAGACGA GGAGGAAAAC 
GTCACCACTC GGGTCGTGCG CCGCAGGGTT ATACTGAAGG GTGATGAGGC TGAGGATCTT 
CCAGGAGAGC AAGTGAGCGA GGAGCAATTC ACAGACGAGC ATGGCAACAT CGTCACCAAA 
AAGCCGGATG TTGTGGATGT GAAGAGAGGA GGTGCTCAGA TAGTCAAATG TGCGAGTCTG 
CGGAGAGTAA AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331795.1
CDS	229..606
Translation	MWALVTELFFSFILLAFMVISCQNVLHIASSSVRSVLAYIHTQLDRELGENEGVADEEENVTTRVVRRRVILKGDEAEDLPGEQVSEEQFTDEHGNIVTKKPDVVDVKRGGAQIVKCASLRRVKQ

Target ORF information:

RefSeq Version	XM_021476120.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X25, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476120.1

ORF Insert Sequence:

ATGTGGGCTC TGGTCACGGA GCTGTTCTTC AGCTTTATTC TGCTCGCCTT TATGGTGATC 
AGCTGCCAGA ATGTTCTGCA TATCGCCAGC AGCTCAGTGC GCTCTGTGCT GGCCTACATA 
CACACTCAGC TGGACCGAGA GCTGGGCGAG AATGAAGGAG TGGCAGACGA GGAGGAAAAC 
GTCACCACTC GGGTCGTGCG CCGCAGGGTT ATACTGAAGG GTGATGAGGC TGAGGATCTT 
CCAGGAGAGC AAGTGAGCGA GGAGCAATTC ACAGACGAGC ATGGCAACAT CGTCACCAAA 
AAGCCGGATG TTGTGGATGT GAAGAGAGGA GGTGCTCAGA TAGTCAAATG TGCGAGTCTG 
CGGAGAGTAA AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_021476121.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331796.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X26, mRNA.

Price & Availability↑

CloneID	ODa142612
Clone ID Related Accession (Same CDS sequence)	XM_021476121.1
Accession Version	XM_021476121.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 294bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin 1, erythrocytic a isoform X25
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAT GTACTCACAA CATTAAAAGA 
GTCCACGGGC AAAAGAAACA TCACAATGGT AACATGGCCT ACATGTTATT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331796.1
CDS	263..556
Translation	MAQAAKHLRKNKDLEALAEQERKEKEEEKSKKRSRSREKKRKAHVVHRWLIDQDNSVSSELPDGQGVWHYDETCTHNIKRVHGQKKHHNGNMAYMLL

Target ORF information:

RefSeq Version	XM_021476121.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X26, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476121.1

ORF Insert Sequence:

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAT GTACTCACAA CATTAAAAGA 
GTCCACGGGC AAAAGAAACA TCACAATGGT AACATGGCCT ACATGTTATT ATAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_021476118.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331793.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X20, mRNA.

Price & Availability↑

CloneID	ODa142609
Clone ID Related Accession (Same CDS sequence)	XM_021476118.1
Accession Version	XM_021476118.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4488bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin 1, erythrocytic a isoform X19
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGAATTGC ACAGCTAACA TTAGGCGAGG AGCTGCTGGG AAACGAGGGC 
GCCAGGTATT TGATGAAGAT GGAGGACATA AAGGACCATG ATGAGGATTT CCTTTCCCCC 
AAGAAAGTTC TAGAGTACGA GGGAGAGTTG GAGAATCATT CTCCTGCTAT CCCGAGGATC 
CCGTGTGTGT CTCCTGAGAC TGTCATGCTG AAAGAGCATG AGATGGAGCA GCAGCACACT 
CCACTTCCAT TGCCCAAAGA GTACGACGAG GATTCTCTGA TTCCCAGCAG TCCTGCAACT 
GAGACTTCAG ACAATGTCAG TCCGGTTGCC AGTCCCATCC ACACCGGTTT TCTGGTGAGC 
TTCATGGTGG ATGCTCGCGG CGGCTCGATG CGCGGCAGCA GACACAACGG CCTCCGCGTC 
ATCATTCCTC CTCGAACCTG CGCCGCTCCC ACACGCATCA CCTGCCGCCT GGTGAAGCCC 
CAGAAGCTGC CCACACCGCC GCCATTAGTG GAGGGAGAGG GTCTCGCCAG CCGCATCATC 
TCCCTTGGAC CCGCCAGCAT GCAGTTTTTA GGGCCTGTGA TTGTGGAGAT TCCTCATTTT 
GCGGCTCTCG GTCGGGGAGA TCGGGAACTC GTGGTCTTGA GGAGTGAGAA CGGATCGGTC 
TGGAAGGAAC ATCGTAACCG ATATGGAGAT GAAGTGCTAG AGACCATCCT CAACGGCATG 
GATGAAGATC TGGAGAGTCA GGAGGAGCTC GGGAAGAAAC GCATCAGACG GATCATCTCC 
ACCGACTTCC CGCTCTACTT TGCGGTGGTG TCTCGCATCC AGCAGGAGAA CGATCTGATT 
GGCCCAGAAG GAGGTTTTCT GTCCAGTAAA CTGGTGCCGA TGGTCCAGGC CACCTTTCCA 
GAGACAGCCG TCACCAAACG CGTGCGTCTG GGACTGCAGG CCCAGCCGGT GCCTGATGAG 
CTGGTGGCCA AGCTTTTGGG GAACCAGGCC ACGTTCAGTC CTGTGGTGAC GGTGGAGCCT 
CGGCGCCGGA AGTTTCACCG TCCCATCGGC CTAAGAATCC CACTGCCACC CTCATGGAGA 
GAGAGCCCGC GGGACGCCGG AGAGGGAGAC ACCACCAGCC TGCGCCTGCT CTGCAGCGTC 
ATCGGTGGAA CAGCTCCTGC CCAGTGGGAA GATATAACTG GCACCACCAA ACTGGCGTAT 
TCCAATGACT GTGCCAGTTT CACCACCAAC GTCTCTGCCC GATTCTGGCT GGCGGACTGT 
CCTCGGACTG CGGAGGCCGT GTCCTTTGCC AACCTCCTGT ACCGCGAGCT GTCGGCCGTG 
CCGTACATGG CCAAGTTTGT AGTGTTTGCG AAGATGAATG AAGCTCGCGA GGGTCGACTC 
CGCTGCTACT GCATGACGGA TGACAAGATG GACAAAACCC TGGAGCAGCA CGAGAACTTC 
TCGGAAGTGG CTCGCAGCAG AGACATCGAG GTGATGGAGG GAATGCCGCT GTATTTGGAG 
TGTTCAGGAA ACTTGGTTCC TATAAGAAAA GCAGCCCAGC AGCCTCGCTG TTTCAGCTTC 
CAGGCCTTTA AAGACAACAG ACTGCCTGTG TCCGTCAAGG TGAGGGACAG CAGTAAAGAC 
CCCTCTGGGT TTTTGTCTTT CTTGCGCAAG TCAACCAAAT ATGAAGACAG CCAACACGTG 
CTGTGCAACC TCAACGTGAC CATGCCGCCG TGCATTAAGA TTATGGGCAG CGATGAGCGG 
AGGAGGACTC TGACTCCTCT GGCTCTCAGA GAAAGATACA GCGCTCTGAA TGAACCGGCA 
ATGGATGTGA GCTGCAGCAT CAGATTTGCT GAACTCTTGA GCCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331793.1
CDS	224..4711
Translation	MAQAAKHLRKNKDLEALAEQERKEKEEEKSKKRSRSREKKRKAHVVHRWLIDQDNSVSSELPDGQGVWHYDETADAATSFLRAARSGNLEKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLINHGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGLTPLHVSSFMGHLNIVKILMQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGANPNAAGKVGLTPLHVAVHHNNLDVVNLLLSKGGSPHSAARNGYTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPLHLASQEGQPDMVLLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKHGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSNGTSALAIAKRLGYISVIDVLKLVTEETVTMTTTEKHRMSFPETVDEILDVSEDEGIAQLTLGEELLGNEGARYLMKMEDIKDHDEDFLSPKKVLEYEGELENHSPAIPRIPCVSPETVMLKEHEMEQQHTPLPLPKEYDEDSLIPSSPATETSDNVSPVASPIHTGFLVSFMVDARGGSMRGSRHNGLRVIIPPRTCAAPTRITCRLVKPQKLPTPPPLVEGEGLASRIISLGPASMQFLGPVIVEIPHFAALGRGDRELVVLRSENGSVWKEHRNRYGDEVLETILNGMDEDLESQEELGKKRIRRIISTDFPLYFAVVSRIQQENDLIGPEGGFLSSKLVPMVQATFPETAVTKRVRLGLQAQPVPDELVAKLLGNQATFSPVVTVEPRRRKFHRPIGLRIPLPPSWRESPRDAGEGDTTSLRLLCSVIGGTAPAQWEDITGTTKLAYSNDCASFTTNVSARFWLADCPRTAEAVSFANLLYRELSAVPYMAKFVVFAKMNEAREGRLRCYCMTDDKMDKTLEQHENFSEVARSRDIEVMEGMPLYLECSGNLVPIRKAAQQPRCFSFQAFKDNRLPVSVKVRDSSKDPSGFLSFLRKSTKYEDSQHVLCNLNVTMPPCIKIMGSDERRRTLTPLALRERYSALNEPAMDVSCSIRFAELLSQ

Target ORF information:

RefSeq Version	XM_021476118.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X20, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476118.1

ORF Insert Sequence:

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGAATTGC ACAGCTAACA TTAGGCGAGG AGCTGCTGGG AAACGAGGGC 
GCCAGGTATT TGATGAAGAT GGAGGACATA AAGGACCATG ATGAGGATTT CCTTTCCCCC 
AAGAAAGTTC TAGAGTACGA GGGAGAGTTG GAGAATCATT CTCCTGCTAT CCCGAGGATC 
CCGTGTGTGT CTCCTGAGAC TGTCATGCTG AAAGAGCATG AGATGGAGCA GCAGCACACT 
CCACTTCCAT TGCCCAAAGA GTACGACGAG GATTCTCTGA TTCCCAGCAG TCCTGCAACT 
GAGACTTCAG ACAATGTCAG TCCGGTTGCC AGTCCCATCC ACACCGGTTT TCTGGTGAGC 
TTCATGGTGG ATGCTCGCGG CGGCTCGATG CGCGGCAGCA GACACAACGG CCTCCGCGTC 
ATCATTCCTC CTCGAACCTG CGCCGCTCCC ACACGCATCA CCTGCCGCCT GGTGAAGCCC 
CAGAAGCTGC CCACACCGCC GCCATTAGTG GAGGGAGAGG GTCTCGCCAG CCGCATCATC 
TCCCTTGGAC CCGCCAGCAT GCAGTTTTTA GGGCCTGTGA TTGTGGAGAT TCCTCATTTT 
GCGGCTCTCG GTCGGGGAGA TCGGGAACTC GTGGTCTTGA GGAGTGAGAA CGGATCGGTC 
TGGAAGGAAC ATCGTAACCG ATATGGAGAT GAAGTGCTAG AGACCATCCT CAACGGCATG 
GATGAAGATC TGGAGAGTCA GGAGGAGCTC GGGAAGAAAC GCATCAGACG GATCATCTCC 
ACCGACTTCC CGCTCTACTT TGCGGTGGTG TCTCGCATCC AGCAGGAGAA CGATCTGATT 
GGCCCAGAAG GAGGTTTTCT GTCCAGTAAA CTGGTGCCGA TGGTCCAGGC CACCTTTCCA 
GAGACAGCCG TCACCAAACG CGTGCGTCTG GGACTGCAGG CCCAGCCGGT GCCTGATGAG 
CTGGTGGCCA AGCTTTTGGG GAACCAGGCC ACGTTCAGTC CTGTGGTGAC GGTGGAGCCT 
CGGCGCCGGA AGTTTCACCG TCCCATCGGC CTAAGAATCC CACTGCCACC CTCATGGAGA 
GAGAGCCCGC GGGACGCCGG AGAGGGAGAC ACCACCAGCC TGCGCCTGCT CTGCAGCGTC 
ATCGGTGGAA CAGCTCCTGC CCAGTGGGAA GATATAACTG GCACCACCAA ACTGGCGTAT 
TCCAATGACT GTGCCAGTTT CACCACCAAC GTCTCTGCCC GATTCTGGCT GGCGGACTGT 
CCTCGGACTG CGGAGGCCGT GTCCTTTGCC AACCTCCTGT ACCGCGAGCT GTCGGCCGTG 
CCGTACATGG CCAAGTTTGT AGTGTTTGCG AAGATGAATG AAGCTCGCGA GGGTCGACTC 
CGCTGCTACT GCATGACGGA TGACAAGATG GACAAAACCC TGGAGCAGCA CGAGAACTTC 
TCGGAAGTGG CTCGCAGCAG AGACATCGAG GTGATGGAGG GAATGCCGCT GTATTTGGAG 
TGTTCAGGAA ACTTGGTTCC TATAAGAAAA GCAGCCCAGC AGCCTCGCTG TTTCAGCTTC 
CAGGCCTTTA AAGACAACAG ACTGCCTGTG TCCGTCAAGG TGAGGGACAG CAGTAAAGAC 
CCCTCTGGGT TTTTGTCTTT CTTGCGCAAG TCAACCAAAT ATGAAGACAG CCAACACGTG 
CTGTGCAACC TCAACGTGAC CATGCCGCCG TGCATTAAGA TTATGGGCAG CGATGAGCGG 
AGGAGGACTC TGACTCCTCT GGCTCTCAGA GAAAGATACA GCGCTCTGAA TGAACCGGCA 
ATGGATGTGA GCTGCAGCAT CAGATTTGCT GAACTCTTGA GCCAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_021476110.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331785.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X12, mRNA.

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CloneID	ODa142602
Clone ID Related Accession (Same CDS sequence)	XM_021476110.1
Accession Version	XM_021476110.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5994bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin 1, erythrocytic a isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGCGAGGA GCTGCTGGGA AACGAGGGCG CCAGGAATCA TTCTCCTGCT 
ATCCCGAGGA TCCCGTGTGT GTCTCCTGAG ACTGTCATGC TGAAAGAGCA TGAGATGGAG 
CAGCAGCACA CTCCACTTCC ATTGCCCAAA GAGTACGACG AGGATTCTCT GATTCCCAGC 
AGTCCTGCAA CTGAGACTTC AGACAATGTC AGTCCGGTTG CCAGTCCCAT CCACACCGGT 
TTTCTGGTGA GCTTCATGGT GGATGCTCGC GGCGGCTCGA TGCGCGGCAG CAGACACAAC 
GGCCTCCGCG TCATCATTCC TCCTCGAACC TGCGCCGCTC CCACACGCAT CACCTGCCGC 
CTGGTGAAGC CCCAGAAGCT GCCCACACCG CCGCCATTAG TGGAGGGAGA GGGTCTCGCC 
AGCCGCATCA TCTCCCTTGG ACCCGCCAGC ATGCAGTTTT TAGGGCCTGT GATTGTGGAG 
ATTCCTCATT TTGCGGCTCT CGGTCGGGGA GATCGGGAAC TCGTGGTCTT GAGGAGTGAG 
AACGGATCGG TCTGGAAGGA ACATCGTAAC CGATATGGAG ATGAAGTGCT AGAGACCATC 
CTCAACGGCA TGGATGAAGA TCTGGAGAGT CAGGAGGAGC TCGGGAAGAA ACGCATCAGA 
CGGATCATCT CCACCGACTT CCCGCTCTAC TTTGCGGTGG TGTCTCGCAT CCAGCAGGAG 
AACGATCTGA TTGGCCCAGA AGGAGGTTTT CTGTCCAGTA AACTGGTGCC GATGGTCCAG 
GCCACCTTTC CAGAGACAGC CGTCACCAAA CGCGTGCGTC TGGGACTGCA GGCCCAGCCG 
GTGCCTGATG AGCTGGTGGC CAAGCTTTTG GGGAACCAGG CCACGTTCAG TCCTGTGGTG 
ACGGTGGAGC CTCGGCGCCG GAAGTTTCAC CGTCCCATCG GCCTAAGAAT CCCACTGCCA 
CCCTCATGGA GAGAGAGCCC GCGGGACGCC GGAGAGGGAG ACACCACCAG CCTGCGCCTG 
CTCTGCAGCG TCATCGGTGG AACAGCTCCT GCCCAGTGGG AAGATATAAC TGGCACCACC 
AAACTGGCGT ATTCCAATGA CTGTGCCAGT TTCACCACCA ACGTCTCTGC CCGATTCTGG 
CTGGCGGACT GTCCTCGGAC TGCGGAGGCC GTGTCCTTTG CCAACCTCCT GTACCGCGAG 
CTGTCGGCCG TGCCGTACAT GGCCAAGTTT GTAGTGTTTG CGAAGATGAA TGAAGCTCGC 
GAGGGTCGAC TCCGCTGCTA CTGCATGACG GATGACAAGA TGGACAAAAC CCTGGAGCAG 
CACGAGAACT TCTCGGAAGT GGCTCGCAGC AGAGACATCG AGGTGATGGA GGGAATGCCG 
CTGTATTTGG AGTGTTCAGG AAACTTGGTT CCTATAAGAA AAGCAGCCCA GCAGCCTCGC 
TGTTTCAGCT TCCAGGCCTT TAAAGACAAC AGACTGCCTG TGTCCGTCAA GGTGAGGGAC 
AGCAGTAAAG ACCCCTCTGG GTTTTTGTCT TTCTTGCGCA AGTCAACCAA ATATGAAGAC 
AGCCAACACG TGCTGTGCAA CCTCAACGTG ACCATGCCGC CGTGCATTAA GATTATGGGC 
AGCGATGAGC GGAGGAGGAC TCTGACTCCT CTGGCTCTCA GAGAAAGATA CAGCGCTCTG 
AATGAACCGG CAATGGCATC ACTGAGCGCC ATGGAAAGGA CGGAGTTAAA GATGGCTTTA 
ATAGCTGAAC AGCTAGGCCT GAGCTGGGCT GAGTTGGGAC GGGAACTGCA GTTTAATGTA 
GATGAAATAA ATAAGATCCG TGTGGAGAAT CCCAACTCCC TTTTAGAGCA GAGCTCAACG 
CTGCTCAACC TGTGGGCCGC CCGAGAGGGG AAGAGAGCCA AAATGGACAG CCTGTACGTG 
GCCCTCAAGA GCATCGACCG AGTGGACATT GTGAACATGA TGGAGGGCCA GGGAGGACCA 
CCGGCGGGTC AGCAGGGGGG CTGGGAGCAG GACGCTTCTA GGTCTAGGCA TCACGACCGA 
GACCATCTCT CACCTGGAAT CACAAATGGT TACGGGCTGG TGGTGCAGGA GGAACTAGTG 
TCACCGGCCT CCATGCAGTA CAGTCTGCCC TCGCCACTGG GCAATGAGCC CTACTGGCAG 
GAGGTTTCCA GCATGGAGTG CGCGCCCATC GCCACCACTG AAGAAGACAC GCTCATGGAG 
ATGTCCGAGG TACAGGTTTG GCCCTCAGGA AATAGTCCGT CCATTGTGGC TGTAGAGGAC 
TCCTCATTGG AGTGCAGCAA TGCGGACGAC TCCGAGGCGC TGCAGAACCG GAGCCTGGGT 
CACTCCAGGA GCGGGACGCC CGGTGGGAAC GGACTCACTC CGGGAGGTTT AAGTGGCTCC 
ATCGAGCTCC TGGAGGATCA GTCCATCGGT GCGGATTCAG ACTACAGTGC CGCCGGGATG 
CTGCGTCTGA GCGAAGGGGT CAACATCAGT GCTCTGGACA GAGGTCAGAG GTCGGAGAGT 
TCAGAAGGGG CTGTAGGTCA CCAAGTCACC AACAACATTA AAGACGGTTC CAATGGAGGT 
TTGGGAGAAG GAGGAGGTGA CGGCACACAG TTAGAAGATA GCATATCTCT CATTTCAGGG 
CAACAGCGTG TGTATGCTCG AATGAGCGAG TCGCCTGGGC TGAGAAGACG GGCAGATCCT 
GGCGAAGACA GGTTAAGCGC AGGGTCGTTC CTGTCTTATC TGCAGGATCA GGGCTCTCCT 
GGTTGGCATT CGGCTGCAGA AGCGACTCAG AACAGATTGG CGTCTTTGCA ATGCTCCCTT 
CCTAGGCAGG CAATAGAGTC TATGATGTCA TCGGTGCGAG CTACGGTGGA CTCTGACTCC 
AAGCAGTCTT GTTTGGGCCA GGAGGCTCTG ATTGAGCCAG TGCGGGACAT GGGGCACTCT 
GAGATCATCC ATGGCCATTA TCAGGGGACT CAGCCGTTTG AGAAAGGACT CGGCTTCCCG 
CACAGAACAA CCAACATGCG CACTTGGGAG GACATGCGTC TGAGCGGACA GGGTGATGAG 
GCTGAGGATC TTCCAGGAGA GCAAGTGAGC GAGGAGCAAT TCACAGACGA GCATGGCAAC 
ATCGTCACCA AAAAGATTGT GAGGAAAGTG GTCCGGAGAG GAAAAGGGGA GGAAGGGGGT 
CAGGAGCTGA TCATTGAAGG TCTGCAGCAG GATATCAGTG AGCCTGACTT TGATGGAGAA 
CAGTTCATGA GCTATGCCGT CCTGGGACGT GACAGCAAGC CGGATGTTGT GGATGTGAAG 
AGAGGAGGTG CTCAGATAGT CAAATGTGCG AGTCTGCGGA GAGTAAAGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331785.1
CDS	222..6215
Translation	MAQAAKHLRKNKDLEALAEQERKEKEEEKSKKRSRSREKKRKAHVVHRWLIDQDNSVSSELPDGQGVWHYDETADAATSFLRAARSGNLEKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLINHGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGLTPLHVSSFMGHLNIVKILMQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGANPNAAGKVGLTPLHVAVHHNNLDVVNLLLSKGGSPHSAARNGYTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPLHLASQEGQPDMVLLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKHGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSNGTSALAIAKRLGYISVIDVLKLVTEETVTMTTTEKHRMSFPETVDEILDVSEDEGEELLGNEGARNHSPAIPRIPCVSPETVMLKEHEMEQQHTPLPLPKEYDEDSLIPSSPATETSDNVSPVASPIHTGFLVSFMVDARGGSMRGSRHNGLRVIIPPRTCAAPTRITCRLVKPQKLPTPPPLVEGEGLASRIISLGPASMQFLGPVIVEIPHFAALGRGDRELVVLRSENGSVWKEHRNRYGDEVLETILNGMDEDLESQEELGKKRIRRIISTDFPLYFAVVSRIQQENDLIGPEGGFLSSKLVPMVQATFPETAVTKRVRLGLQAQPVPDELVAKLLGNQATFSPVVTVEPRRRKFHRPIGLRIPLPPSWRESPRDAGEGDTTSLRLLCSVIGGTAPAQWEDITGTTKLAYSNDCASFTTNVSARFWLADCPRTAEAVSFANLLYRELSAVPYMAKFVVFAKMNEAREGRLRCYCMTDDKMDKTLEQHENFSEVARSRDIEVMEGMPLYLECSGNLVPIRKAAQQPRCFSFQAFKDNRLPVSVKVRDSSKDPSGFLSFLRKSTKYEDSQHVLCNLNVTMPPCIKIMGSDERRRTLTPLALRERYSALNEPAMASLSAMERTELKMALIAEQLGLSWAELGRELQFNVDEINKIRVENPNSLLEQSSTLLNLWAAREGKRAKMDSLYVALKSIDRVDIVNMMEGQGGPPAGQQGGWEQDASRSRHHDRDHLSPGITNGYGLVVQEELVSPASMQYSLPSPLGNEPYWQEVSSMECAPIATTEEDTLMEMSEVQVWPSGNSPSIVAVEDSSLECSNADDSEALQNRSLGHSRSGTPGGNGLTPGGLSGSIELLEDQSIGADSDYSAAGMLRLSEGVNISALDRGQRSESSEGAVGHQVTNNIKDGSNGGLGEGGGDGTQLEDSISLISGQQRVYARMSESPGLRRRADPGEDRLSAGSFLSYLQDQGSPGWHSAAEATQNRLASLQCSLPRQAIESMMSSVRATVDSDSKQSCLGQEALIEPVRDMGHSEIIHGHYQGTQPFEKGLGFPHRTTNMRTWEDMRLSGQGDEAEDLPGEQVSEEQFTDEHGNIVTKKIVRKVVRRGKGEEGGQELIIEGLQQDISEPDFDGEQFMSYAVLGRDSKPDVVDVKRGGAQIVKCASLRRVKQ

Target ORF information:

RefSeq Version	XM_021476110.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476110.1

ORF Insert Sequence:

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGCGAGGA GCTGCTGGGA AACGAGGGCG CCAGGAATCA TTCTCCTGCT 
ATCCCGAGGA TCCCGTGTGT GTCTCCTGAG ACTGTCATGC TGAAAGAGCA TGAGATGGAG 
CAGCAGCACA CTCCACTTCC ATTGCCCAAA GAGTACGACG AGGATTCTCT GATTCCCAGC 
AGTCCTGCAA CTGAGACTTC AGACAATGTC AGTCCGGTTG CCAGTCCCAT CCACACCGGT 
TTTCTGGTGA GCTTCATGGT GGATGCTCGC GGCGGCTCGA TGCGCGGCAG CAGACACAAC 
GGCCTCCGCG TCATCATTCC TCCTCGAACC TGCGCCGCTC CCACACGCAT CACCTGCCGC 
CTGGTGAAGC CCCAGAAGCT GCCCACACCG CCGCCATTAG TGGAGGGAGA GGGTCTCGCC 
AGCCGCATCA TCTCCCTTGG ACCCGCCAGC ATGCAGTTTT TAGGGCCTGT GATTGTGGAG 
ATTCCTCATT TTGCGGCTCT CGGTCGGGGA GATCGGGAAC TCGTGGTCTT GAGGAGTGAG 
AACGGATCGG TCTGGAAGGA ACATCGTAAC CGATATGGAG ATGAAGTGCT AGAGACCATC 
CTCAACGGCA TGGATGAAGA TCTGGAGAGT CAGGAGGAGC TCGGGAAGAA ACGCATCAGA 
CGGATCATCT CCACCGACTT CCCGCTCTAC TTTGCGGTGG TGTCTCGCAT CCAGCAGGAG 
AACGATCTGA TTGGCCCAGA AGGAGGTTTT CTGTCCAGTA AACTGGTGCC GATGGTCCAG 
GCCACCTTTC CAGAGACAGC CGTCACCAAA CGCGTGCGTC TGGGACTGCA GGCCCAGCCG 
GTGCCTGATG AGCTGGTGGC CAAGCTTTTG GGGAACCAGG CCACGTTCAG TCCTGTGGTG 
ACGGTGGAGC CTCGGCGCCG GAAGTTTCAC CGTCCCATCG GCCTAAGAAT CCCACTGCCA 
CCCTCATGGA GAGAGAGCCC GCGGGACGCC GGAGAGGGAG ACACCACCAG CCTGCGCCTG 
CTCTGCAGCG TCATCGGTGG AACAGCTCCT GCCCAGTGGG AAGATATAAC TGGCACCACC 
AAACTGGCGT ATTCCAATGA CTGTGCCAGT TTCACCACCA ACGTCTCTGC CCGATTCTGG 
CTGGCGGACT GTCCTCGGAC TGCGGAGGCC GTGTCCTTTG CCAACCTCCT GTACCGCGAG 
CTGTCGGCCG TGCCGTACAT GGCCAAGTTT GTAGTGTTTG CGAAGATGAA TGAAGCTCGC 
GAGGGTCGAC TCCGCTGCTA CTGCATGACG GATGACAAGA TGGACAAAAC CCTGGAGCAG 
CACGAGAACT TCTCGGAAGT GGCTCGCAGC AGAGACATCG AGGTGATGGA GGGAATGCCG 
CTGTATTTGG AGTGTTCAGG AAACTTGGTT CCTATAAGAA AAGCAGCCCA GCAGCCTCGC 
TGTTTCAGCT TCCAGGCCTT TAAAGACAAC AGACTGCCTG TGTCCGTCAA GGTGAGGGAC 
AGCAGTAAAG ACCCCTCTGG GTTTTTGTCT TTCTTGCGCA AGTCAACCAA ATATGAAGAC 
AGCCAACACG TGCTGTGCAA CCTCAACGTG ACCATGCCGC CGTGCATTAA GATTATGGGC 
AGCGATGAGC GGAGGAGGAC TCTGACTCCT CTGGCTCTCA GAGAAAGATA CAGCGCTCTG 
AATGAACCGG CAATGGCATC ACTGAGCGCC ATGGAAAGGA CGGAGTTAAA GATGGCTTTA 
ATAGCTGAAC AGCTAGGCCT GAGCTGGGCT GAGTTGGGAC GGGAACTGCA GTTTAATGTA 
GATGAAATAA ATAAGATCCG TGTGGAGAAT CCCAACTCCC TTTTAGAGCA GAGCTCAACG 
CTGCTCAACC TGTGGGCCGC CCGAGAGGGG AAGAGAGCCA AAATGGACAG CCTGTACGTG 
GCCCTCAAGA GCATCGACCG AGTGGACATT GTGAACATGA TGGAGGGCCA GGGAGGACCA 
CCGGCGGGTC AGCAGGGGGG CTGGGAGCAG GACGCTTCTA GGTCTAGGCA TCACGACCGA 
GACCATCTCT CACCTGGAAT CACAAATGGT TACGGGCTGG TGGTGCAGGA GGAACTAGTG 
TCACCGGCCT CCATGCAGTA CAGTCTGCCC TCGCCACTGG GCAATGAGCC CTACTGGCAG 
GAGGTTTCCA GCATGGAGTG CGCGCCCATC GCCACCACTG AAGAAGACAC GCTCATGGAG 
ATGTCCGAGG TACAGGTTTG GCCCTCAGGA AATAGTCCGT CCATTGTGGC TGTAGAGGAC 
TCCTCATTGG AGTGCAGCAA TGCGGACGAC TCCGAGGCGC TGCAGAACCG GAGCCTGGGT 
CACTCCAGGA GCGGGACGCC CGGTGGGAAC GGACTCACTC CGGGAGGTTT AAGTGGCTCC 
ATCGAGCTCC TGGAGGATCA GTCCATCGGT GCGGATTCAG ACTACAGTGC CGCCGGGATG 
CTGCGTCTGA GCGAAGGGGT CAACATCAGT GCTCTGGACA GAGGTCAGAG GTCGGAGAGT 
TCAGAAGGGG CTGTAGGTCA CCAAGTCACC AACAACATTA AAGACGGTTC CAATGGAGGT 
TTGGGAGAAG GAGGAGGTGA CGGCACACAG TTAGAAGATA GCATATCTCT CATTTCAGGG 
CAACAGCGTG TGTATGCTCG AATGAGCGAG TCGCCTGGGC TGAGAAGACG GGCAGATCCT 
GGCGAAGACA GGTTAAGCGC AGGGTCGTTC CTGTCTTATC TGCAGGATCA GGGCTCTCCT 
GGTTGGCATT CGGCTGCAGA AGCGACTCAG AACAGATTGG CGTCTTTGCA ATGCTCCCTT 
CCTAGGCAGG CAATAGAGTC TATGATGTCA TCGGTGCGAG CTACGGTGGA CTCTGACTCC 
AAGCAGTCTT GTTTGGGCCA GGAGGCTCTG ATTGAGCCAG TGCGGGACAT GGGGCACTCT 
GAGATCATCC ATGGCCATTA TCAGGGGACT CAGCCGTTTG AGAAAGGACT CGGCTTCCCG 
CACAGAACAA CCAACATGCG CACTTGGGAG GACATGCGTC TGAGCGGACA GGGTGATGAG 
GCTGAGGATC TTCCAGGAGA GCAAGTGAGC GAGGAGCAAT TCACAGACGA GCATGGCAAC 
ATCGTCACCA AAAAGATTGT GAGGAAAGTG GTCCGGAGAG GAAAAGGGGA GGAAGGGGGT 
CAGGAGCTGA TCATTGAAGG TCTGCAGCAG GATATCAGTG AGCCTGACTT TGATGGAGAA 
CAGTTCATGA GCTATGCCGT CCTGGGACGT GACAGCAAGC CGGATGTTGT GGATGTGAAG 
AGAGGAGGTG CTCAGATAGT CAAATGTGCG AGTCTGCGGA GAGTAAAGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_021476111.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331786.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X13, mRNA.

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CloneID	ODa142603
Clone ID Related Accession (Same CDS sequence)	XM_021476111.1
Accession Version	XM_021476111.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5970bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin 1, erythrocytic a isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGAATTGC ACAGCTAACA TTAGGCGAGG AGCTGCTGGG AAACGAGGGC 
GCCAGGTATT TGATGAAGAT GGAGGACATA AAGGACCATG ATGAGGATTT CCTTTCCCCC 
AAGAAAGTTC TAGAGTACGA GGGAGAGTTG GAGAATCATT CTCCTGCTAT CCCGAGGATC 
CCGTGTGTGT CTCCTGAGAC TGTCATGCTG AAAGAGCATG AGATGGAGCA GCAGCACACT 
CCACTTCCAT TGCCCAAAGA GTACGACGAG GATTCTCTGA TTCCCAGCAG TCCTGCAACT 
GAGACTTCAG ACAATGTCAG TCCGGTTGCC AGTCCCATCC ACACCGGTTT TCTGGTGAGC 
TTCATGGTGG ATGCTCGCGG CGGCTCGATG CGCGGCAGCA GACACAACGG CCTCCGCGTC 
ATCATTCCTC CTCGAACCTG CGCCGCTCCC ACACGCATCA CCTGCCGCCT GGTGAAGCCC 
CAGAAGCTGC CCACACCGCC GCCATTAGTG GAGGGAGAGG GTCTCGCCAG CCGCATCATC 
TCCCTTGGAC CCGCCAGCAT GCAGTTTTTA GGGCCTGTGA TTGTGGAGAT TCCTCATTTT 
GCGGCTCTCG GTCGGGGAGA TCGGGAACTC GTGGTCTTGA GGAGTGAGAA CGGATCGGTC 
TGGAAGGAAC ATCGTAACCG ATATGGAGAT GAAGTGCTAG AGACCATCCT CAACGGCATG 
GATGAAGATC TGGAGAGTCA GGAGGAGCTC GGGAAGAAAC GCATCAGACG GATCATCTCC 
ACCGACTTCC CGCTCTACTT TGCGGTGGTG TCTCGCATCC AGCAGGAGAA CGATCTGATT 
GGCCCAGAAG GAGGTTTTCT GTCCAGTAAA CTGGTGCCGA TGGTCCAGGC CACCTTTCCA 
GAGACAGCCG TCACCAAACG CGTGCGTCTG GGACTGCAGG CCCAGCCGGT GCCTGATGAG 
CTGGTGGCCA AGCTTTTGGG GAACCAGGCC ACGTTCAGTC CTGTGGTGAC GGTGGAGCCT 
CGGCGCCGGA AGTTTCACCG TCCCATCGGC CTAAGAATCC CACTGCCACC CTCATGGAGA 
GAGAGCCCGC GGGACGCCGG AGAGGGAGAC ACCACCAGCC TGCGCCTGCT CTGCAGCGTC 
ATCGGTGGAA CAGCTCCTGC CCAGTGGGAA GATATAACTG GCACCACCAA ACTGGCGTAT 
TCCAATGACT GTGCCAGTTT CACCACCAAC GTCTCTGCCC GATTCTGGCT GGCGGACTGT 
CCTCGGACTG CGGAGGCCGT GTCCTTTGCC AACCTCCTGT ACCGCGAGCT GTCGGCCGTG 
CCGTACATGG CCAAGTTTGT AGTGTTTGCG AAGATGAATG AAGCTCGCGA GGGTCGACTC 
CGCTGCTACT GCATGACGGA TGACAAGATG GACAAAACCC TGGAGCAGCA CGAGAACTTC 
TCGGAAGTGG CTCGCAGCAG AGACATCGAG GTGATGGAGG GAATGCCGCT GTATTTGGAG 
TGTTCAGGAA ACTTGGTTCC TATAAGAAAA GCAGCCCAGC AGCCTCGCTG TTTCAGCTTC 
CAGGCCTTTA AAGACAACAG ACTGCCTGTG TCCGTCAAGG TGAGGGACAG CAGTAAAGAC 
CCCTCTGGGT TTTTGTCTTT CTTGCGCAAG TCAACCAAAT ATGAAGACAG CCAACACGTG 
CTGTGCAACC TCAACGTGAC CATGCCGCCG TGCATTAAGA TTATGGGCAG CGATGAGCGG 
AGGAGGACTC TGACTCCTCT GGCTCTCAGA GAAAGATACA GCGCTCTGAA TGAACCGGCA 
ATGGCATCAC TGAGCGCCAT GGAAAGGACG GAGTTAAAGA TGGCTTTAAT AGCTGAACAG 
CTAGGCCTGA GCTGGGCTGT GGACAGCCTG TACGTGGCCC TCAAGAGCAT CGACCGAGTG 
GACATTGTGA ACATGATGGA GGGCCAGGGA GGACCACCGG CGGGTCAGCA GGGGGGCTGG 
GAGCAGGACG CTTCTAGGTC TAGGCATCAC GACCGAGACC ATCTCTCACC TGGAATCACA 
AATGGTTACG GGCTGGTGGT GCAGGAGGAA CTAGTGTCAC CGGCCTCCAT GCAGTACAGT 
CTGCCCTCGC CACTGGGCAA TGAGCCCTAC TGGCAGGAGG TTTCCAGCAT GGAGTGCGCG 
CCCATCGCCA CCACTGAAGA AGACACGCTC ATGGAGATGT CCGAGGTACA GGTTTGGCCC 
TCAGGAAATA GTCCGTCCAT TGTGGCTGTA GAGGACTCCT CATTGGAGTG CAGCAATGCG 
GACGACTCCG AGGCGCTGCA GAACCGGAGC CTGGGTCACT CCAGGAGCGG GACGCCCGGT 
GGGAACGGAC TCACTCCGGG AGGTTTAAGT GGCTCCATCG AGCTCCTGGA GGATCAGTCC 
ATCGGTGCGG ATTCAGACTA CAGTGCCGCC GGGATGCTGC GTCTGAGCGA AGGGGTCAAC 
ATCAGTGCTC TGGACAGAGG TCAGAGGTCG GAGAGTTCAG AAGGGGCTGT AGGTCACCAA 
GTCACCAACA ACATTAAAGA CGGTTCCAAT GGAGGTTTGG GAGAAGGAGG AGGTGACGGC 
ACACAGTTAG AAGATAGCAT ATCTCTCATT TCAGGGCAAC AGCGTGTGTA TGCTCGAATG 
AGCGAGTCGC CTGGGCTGAG AAGACGGGCA GATCCTGGCG AAGACAGGTT AAGCGCAGGG 
TCGTTCCTGT CTTATCTGCA GGATCAGGGC TCTCCTGGTT GGCATTCGGC TGCAGAAGCG 
ACTCAGAACA GATTGGCGTC TTTGCAATGC TCCCTTCCTA GGCAGGCAAT AGAGTCTATG 
ATGTCATCGG TGCGAGCTAC GGTGGACTCT GACTCCAAGC AGTCTTGTTT GGGCCAGGAG 
GCTCTGATTG AGCCAGTGCG GGACATGGGG CACTCTGAGA TCATCCATGG CCATTATCAG 
GGGACTCAGC CGTTTGAGAA AGGACTCGGC TTCCCGCACA GAACAACCAA CATGCGCACT 
TGGGAGGACA TGCGTCTGAG CGGACAGGGT GATGAGGCTG AGGATCTTCC AGGAGAGCAA 
GTGAGCGAGG AGCAATTCAC AGACGAGCAT GGCAACATCG TCACCAAAAA GATTGTGAGG 
AAAGTGGTCC GGAGAGGAAA AGGGGAGGAA GGGGGTCAGG AGCTGATCAT TGAAGGTCTG 
CAGCAGGATA TCAGTGAGCC TGACTTTGAT GGAGAACAGT TCATGAGCTA TGCCGTCCTG 
GGACGTGACA GCAAGCCGGA TGTTGTGGAT GTGAAGAGAG GAGGTGCTCA GATAGTCAAA 
TGTGCGAGTC TGCGGAGAGT AAAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331786.1
CDS	222..6191
Translation	MAQAAKHLRKNKDLEALAEQERKEKEEEKSKKRSRSREKKRKAHVVHRWLIDQDNSVSSELPDGQGVWHYDETADAATSFLRAARSGNLEKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLINHGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGLTPLHVSSFMGHLNIVKILMQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGANPNAAGKVGLTPLHVAVHHNNLDVVNLLLSKGGSPHSAARNGYTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPLHLASQEGQPDMVLLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKHGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSNGTSALAIAKRLGYISVIDVLKLVTEETVTMTTTEKHRMSFPETVDEILDVSEDEGIAQLTLGEELLGNEGARYLMKMEDIKDHDEDFLSPKKVLEYEGELENHSPAIPRIPCVSPETVMLKEHEMEQQHTPLPLPKEYDEDSLIPSSPATETSDNVSPVASPIHTGFLVSFMVDARGGSMRGSRHNGLRVIIPPRTCAAPTRITCRLVKPQKLPTPPPLVEGEGLASRIISLGPASMQFLGPVIVEIPHFAALGRGDRELVVLRSENGSVWKEHRNRYGDEVLETILNGMDEDLESQEELGKKRIRRIISTDFPLYFAVVSRIQQENDLIGPEGGFLSSKLVPMVQATFPETAVTKRVRLGLQAQPVPDELVAKLLGNQATFSPVVTVEPRRRKFHRPIGLRIPLPPSWRESPRDAGEGDTTSLRLLCSVIGGTAPAQWEDITGTTKLAYSNDCASFTTNVSARFWLADCPRTAEAVSFANLLYRELSAVPYMAKFVVFAKMNEAREGRLRCYCMTDDKMDKTLEQHENFSEVARSRDIEVMEGMPLYLECSGNLVPIRKAAQQPRCFSFQAFKDNRLPVSVKVRDSSKDPSGFLSFLRKSTKYEDSQHVLCNLNVTMPPCIKIMGSDERRRTLTPLALRERYSALNEPAMASLSAMERTELKMALIAEQLGLSWAVDSLYVALKSIDRVDIVNMMEGQGGPPAGQQGGWEQDASRSRHHDRDHLSPGITNGYGLVVQEELVSPASMQYSLPSPLGNEPYWQEVSSMECAPIATTEEDTLMEMSEVQVWPSGNSPSIVAVEDSSLECSNADDSEALQNRSLGHSRSGTPGGNGLTPGGLSGSIELLEDQSIGADSDYSAAGMLRLSEGVNISALDRGQRSESSEGAVGHQVTNNIKDGSNGGLGEGGGDGTQLEDSISLISGQQRVYARMSESPGLRRRADPGEDRLSAGSFLSYLQDQGSPGWHSAAEATQNRLASLQCSLPRQAIESMMSSVRATVDSDSKQSCLGQEALIEPVRDMGHSEIIHGHYQGTQPFEKGLGFPHRTTNMRTWEDMRLSGQGDEAEDLPGEQVSEEQFTDEHGNIVTKKIVRKVVRRGKGEEGGQELIIEGLQQDISEPDFDGEQFMSYAVLGRDSKPDVVDVKRGGAQIVKCASLRRVKQ

Target ORF information:

RefSeq Version	XM_021476111.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476111.1

ORF Insert Sequence:

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGAATTGC ACAGCTAACA TTAGGCGAGG AGCTGCTGGG AAACGAGGGC 
GCCAGGTATT TGATGAAGAT GGAGGACATA AAGGACCATG ATGAGGATTT CCTTTCCCCC 
AAGAAAGTTC TAGAGTACGA GGGAGAGTTG GAGAATCATT CTCCTGCTAT CCCGAGGATC 
CCGTGTGTGT CTCCTGAGAC TGTCATGCTG AAAGAGCATG AGATGGAGCA GCAGCACACT 
CCACTTCCAT TGCCCAAAGA GTACGACGAG GATTCTCTGA TTCCCAGCAG TCCTGCAACT 
GAGACTTCAG ACAATGTCAG TCCGGTTGCC AGTCCCATCC ACACCGGTTT TCTGGTGAGC 
TTCATGGTGG ATGCTCGCGG CGGCTCGATG CGCGGCAGCA GACACAACGG CCTCCGCGTC 
ATCATTCCTC CTCGAACCTG CGCCGCTCCC ACACGCATCA CCTGCCGCCT GGTGAAGCCC 
CAGAAGCTGC CCACACCGCC GCCATTAGTG GAGGGAGAGG GTCTCGCCAG CCGCATCATC 
TCCCTTGGAC CCGCCAGCAT GCAGTTTTTA GGGCCTGTGA TTGTGGAGAT TCCTCATTTT 
GCGGCTCTCG GTCGGGGAGA TCGGGAACTC GTGGTCTTGA GGAGTGAGAA CGGATCGGTC 
TGGAAGGAAC ATCGTAACCG ATATGGAGAT GAAGTGCTAG AGACCATCCT CAACGGCATG 
GATGAAGATC TGGAGAGTCA GGAGGAGCTC GGGAAGAAAC GCATCAGACG GATCATCTCC 
ACCGACTTCC CGCTCTACTT TGCGGTGGTG TCTCGCATCC AGCAGGAGAA CGATCTGATT 
GGCCCAGAAG GAGGTTTTCT GTCCAGTAAA CTGGTGCCGA TGGTCCAGGC CACCTTTCCA 
GAGACAGCCG TCACCAAACG CGTGCGTCTG GGACTGCAGG CCCAGCCGGT GCCTGATGAG 
CTGGTGGCCA AGCTTTTGGG GAACCAGGCC ACGTTCAGTC CTGTGGTGAC GGTGGAGCCT 
CGGCGCCGGA AGTTTCACCG TCCCATCGGC CTAAGAATCC CACTGCCACC CTCATGGAGA 
GAGAGCCCGC GGGACGCCGG AGAGGGAGAC ACCACCAGCC TGCGCCTGCT CTGCAGCGTC 
ATCGGTGGAA CAGCTCCTGC CCAGTGGGAA GATATAACTG GCACCACCAA ACTGGCGTAT 
TCCAATGACT GTGCCAGTTT CACCACCAAC GTCTCTGCCC GATTCTGGCT GGCGGACTGT 
CCTCGGACTG CGGAGGCCGT GTCCTTTGCC AACCTCCTGT ACCGCGAGCT GTCGGCCGTG 
CCGTACATGG CCAAGTTTGT AGTGTTTGCG AAGATGAATG AAGCTCGCGA GGGTCGACTC 
CGCTGCTACT GCATGACGGA TGACAAGATG GACAAAACCC TGGAGCAGCA CGAGAACTTC 
TCGGAAGTGG CTCGCAGCAG AGACATCGAG GTGATGGAGG GAATGCCGCT GTATTTGGAG 
TGTTCAGGAA ACTTGGTTCC TATAAGAAAA GCAGCCCAGC AGCCTCGCTG TTTCAGCTTC 
CAGGCCTTTA AAGACAACAG ACTGCCTGTG TCCGTCAAGG TGAGGGACAG CAGTAAAGAC 
CCCTCTGGGT TTTTGTCTTT CTTGCGCAAG TCAACCAAAT ATGAAGACAG CCAACACGTG 
CTGTGCAACC TCAACGTGAC CATGCCGCCG TGCATTAAGA TTATGGGCAG CGATGAGCGG 
AGGAGGACTC TGACTCCTCT GGCTCTCAGA GAAAGATACA GCGCTCTGAA TGAACCGGCA 
ATGGCATCAC TGAGCGCCAT GGAAAGGACG GAGTTAAAGA TGGCTTTAAT AGCTGAACAG 
CTAGGCCTGA GCTGGGCTGT GGACAGCCTG TACGTGGCCC TCAAGAGCAT CGACCGAGTG 
GACATTGTGA ACATGATGGA GGGCCAGGGA GGACCACCGG CGGGTCAGCA GGGGGGCTGG 
GAGCAGGACG CTTCTAGGTC TAGGCATCAC GACCGAGACC ATCTCTCACC TGGAATCACA 
AATGGTTACG GGCTGGTGGT GCAGGAGGAA CTAGTGTCAC CGGCCTCCAT GCAGTACAGT 
CTGCCCTCGC CACTGGGCAA TGAGCCCTAC TGGCAGGAGG TTTCCAGCAT GGAGTGCGCG 
CCCATCGCCA CCACTGAAGA AGACACGCTC ATGGAGATGT CCGAGGTACA GGTTTGGCCC 
TCAGGAAATA GTCCGTCCAT TGTGGCTGTA GAGGACTCCT CATTGGAGTG CAGCAATGCG 
GACGACTCCG AGGCGCTGCA GAACCGGAGC CTGGGTCACT CCAGGAGCGG GACGCCCGGT 
GGGAACGGAC TCACTCCGGG AGGTTTAAGT GGCTCCATCG AGCTCCTGGA GGATCAGTCC 
ATCGGTGCGG ATTCAGACTA CAGTGCCGCC GGGATGCTGC GTCTGAGCGA AGGGGTCAAC 
ATCAGTGCTC TGGACAGAGG TCAGAGGTCG GAGAGTTCAG AAGGGGCTGT AGGTCACCAA 
GTCACCAACA ACATTAAAGA CGGTTCCAAT GGAGGTTTGG GAGAAGGAGG AGGTGACGGC 
ACACAGTTAG AAGATAGCAT ATCTCTCATT TCAGGGCAAC AGCGTGTGTA TGCTCGAATG 
AGCGAGTCGC CTGGGCTGAG AAGACGGGCA GATCCTGGCG AAGACAGGTT AAGCGCAGGG 
TCGTTCCTGT CTTATCTGCA GGATCAGGGC TCTCCTGGTT GGCATTCGGC TGCAGAAGCG 
ACTCAGAACA GATTGGCGTC TTTGCAATGC TCCCTTCCTA GGCAGGCAAT AGAGTCTATG 
ATGTCATCGG TGCGAGCTAC GGTGGACTCT GACTCCAAGC AGTCTTGTTT GGGCCAGGAG 
GCTCTGATTG AGCCAGTGCG GGACATGGGG CACTCTGAGA TCATCCATGG CCATTATCAG 
GGGACTCAGC CGTTTGAGAA AGGACTCGGC TTCCCGCACA GAACAACCAA CATGCGCACT 
TGGGAGGACA TGCGTCTGAG CGGACAGGGT GATGAGGCTG AGGATCTTCC AGGAGAGCAA 
GTGAGCGAGG AGCAATTCAC AGACGAGCAT GGCAACATCG TCACCAAAAA GATTGTGAGG 
AAAGTGGTCC GGAGAGGAAA AGGGGAGGAA GGGGGTCAGG AGCTGATCAT TGAAGGTCTG 
CAGCAGGATA TCAGTGAGCC TGACTTTGAT GGAGAACAGT TCATGAGCTA TGCCGTCCTG 
GGACGTGACA GCAAGCCGGA TGTTGTGGAT GTGAAGAGAG GAGGTGCTCA GATAGTCAAA 
TGTGCGAGTC TGCGGAGAGT AAAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_021476112.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331787.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X14, mRNA.

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CloneID	ODa142604
Clone ID Related Accession (Same CDS sequence)	XM_021476112.1
Accession Version	XM_021476112.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5958bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin 1, erythrocytic a isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGAATTGC ACAGCTAACA TTAGGCGAGG AGCTGCTGGG AAACGAGGGC 
GCCAGGTATT TGATGAAGAT GGAGGACATA AAGGACCATG ATGAGGATTT CCTTTCCCCC 
AAGAAAGTTC TAGAGTACGA GGGAGAGTTG GAGAATCATT CTCCTGCTAT CCCGAGGATC 
CCGTGTGTGT CTCCTGAGAC TGTCATGCTG AAAGAGCATG AGATGGAGCA GCAGCACACT 
CCACTTCCAT TGCCCAAAGA GTACGACGAG GATTCTCTGA TTCCCAGCAG TCCTGCAACT 
GAGACTTCAG ACAATGTCAG TCCGGTTGCC AGTCCCATCC ACACCGGTTT TCTGGTGAGC 
TTCATGGTGG ATGCTCGCGG CGGCTCGATG CGCGGCAGCA GACACAACGG CCTCCGCGTC 
ATCATTCCTC CTCGAACCTG CGCCGCTCCC ACACGCATCA CCTGCCGCCT GGTGAAGCCC 
CAGAAGCTGC CCACACCGCC GCCATTAGTG GAGGGAGAGG GTCTCGCCAG CCGCATCATC 
TCCCTTGGAC CCGCCAGCAT GCAGTTTTTA GGGCCTGTGA TTGTGGAGAT TCCTCATTTT 
GCGGCTCTCG GTCGGGGAGA TCGGGAACTC GTGGTCTTGA GGAGTGAGAA CGGATCGGTC 
TGGAAGGAAC ATCGTAACCG ATATGGAGAT GAAGTGCTAG AGACCATCCT CAACGGCATG 
GATGAAGATC TGGAGAGTCA GGAGGAGCTC GGGAAGAAAC GCATCAGACG GATCATCTCC 
ACCGACTTCC CGCTCTACTT TGCGGTGGTG TCTCGCATCC AGCAGGAGAA CGATCTGATT 
GGCCCAGAAG GAGGTTTTCT GTCCAGTAAA CTGGTGCCGA TGGTCCAGGC CACCTTTCCA 
GAGACAGCCG TCACCAAACG CGTGCGTCTG GGACTGCAGG CCCAGCCGGT GCCTGATGAG 
CTGGTGGCCA AGCTTTTGGG GAACCAGGCC ACGTTCAGTC CTGTGGTGAC GGTGGAGCCT 
CGGCGCCGGA AGTTTCACCG TCCCATCGGC CTAAGAATCC CACTGCCACC CTCATGGAGA 
GAGAGCCCGC GGGACGCCGG AGAGGGAGAC ACCACCAGCC TGCGCCTGCT CTGCAGCGTC 
ATCGGTGGAA CAGCTCCTGC CCAGTGGGAA GATATAACTG GCACCACCAA ACTGGCGTAT 
TCCAATGACT GTGCCAGTTT CACCACCAAC GTCTCTGCCC GATTCTGGCT GGCGGACTGT 
CCTCGGACTG CGGAGGCCGT GTCCTTTGCC AACCTCCTGT ACCGCGAGCT GTCGGCCGTG 
CCGTACATGG CCAAGTTTGT AGTGTTTGCG AAGATGAATG AAGCTCGCGA GGGTCGACTC 
CGCTGCTACT GCATGACGGA TGACAAGATG GACAAAACCC TGGAGCAGCA CGAGAACTTC 
TCGGAAGTGG CTCGCAGCAG AGACATCGAG GTGATGGAGG GAATGCCGCT GTATTTGGAG 
TGTTCAGGAA ACTTGGTTCC TATAAGAAAA GCAGCCCAGC AGCCTCGCTG TTTCAGCTTC 
CAGGCCTTTA AAGACAACAG ACTGCCTGTG TCCGTCAAGG TGAGGGACAG CAGTAAAGAC 
CCCTCTGGGT TTTTGTCTTT CTTGCGCAAG TCAACCAAAT ATGAAGACAG CCAACACGTG 
CTGTGCAACC TCAACGTGAC CATGCCGCCG TGCATTAAGA TTATGGGCAG CGATGAGCGG 
AGGAGGACTC TGACTCCTCT GGCTCTCAGA GAAAGATACA GCGCTCTGAA TGAACCGGCA 
ATGGCATCAC TGAGCGCCAT GGAAAGGACG GAGTTAAAGA TGGCTTTAAT AGCTGAACAG 
CTAGGCCTGA GCTGGGCTGA GTTGGGACGG GAACTGCAGT TTAATGTAGA TGAAATAAAT 
AAGATCCGTG TGGAGAATCC CAACTCCCTT TTAGAGCAGA GCTCAACGCT GCTCAACCTG 
TGGGCCGCCC GAGAGGGGAA GAGAGCCAAA ATGGACAGCC TGTACGTGGC CCTCAAGAGC 
ATCGACCGAG TGGACATTGT GAACATGATG GAGGGCCAGG GAGGACCACC GGCGGGTCAG 
CAGGGGGGCT GGGAGCAGGA CGCTTCTAGG TCTAGGCATC ACGACCGAGA CCATCTCTCA 
CCTGGAATCA CAAATGGTTA CGGGCTGGTG GTGCAGGAGG AACTAGTGTC ACCGGCCTCC 
ATGCAGTACA GTCTGCCCTC GCCACTGGGC AATGAGCCCT ACTGGCAGGA GGTTTCCAGC 
ATGGAGTGCG CGCCCATCGC CACCACTGAA GAAGACACGC TCATGGAGAT GTCCGAGGTA 
CAGGTTTGGC CCTCAGGAAA TAGTCCGTCC ATTGTGGCTG TAGAGGACTC CTCATTGGAG 
TGCAGCAATG CGGACGACTC CGAGGCGCTG CAGAACCGGA GCCTGGGTCA CTCCAGGAGC 
GGGACGCCCG GTGGGAACGG ACTCACTCCG GGAGGTTTAA GTGGCTCCAT CGAGCTCCTG 
GAGGATCAGT CCATCGGTGC GGATTCAGAC TACAGTGCCG CCGGGATGCT GCGTCTGAGC 
GAAGGGGTCA ACATCAGTGC TCTGGACAGA GGTCAGAGGT CGGAGAGTTC AGAAGGGGCT 
GTAGGTCACC AAGTCACCAA CAACATTAAA GACGGTTCCA ATGGAGGTTT GGGAGAAGGA 
GGAGGTGACG GCACACAGTT AGAAGATAGC ATATCTCTCA TTTCAGGGCA ACAGCGTGTG 
TATGCTCGAA TGAGCGAGTC GCCTGGGCTG AGAAGACGGG CAGATCCTGG CGAAGACAGG 
TTAAGCGCAG GGTCGTTCCT GTCTTATCTG CAGGATCAGG GCTCTCCTGG TTGGCATTCG 
GCTGCAGAAG CGACTCAGAA CAGATTGGCG TCTTTGCAAT GCTCCCTTCC TAGGCAGGCA 
ATAGAGTCTA TGATGTCATC GGTGCGAGCT ACGGTGGACT CTGACTCCAA GCAGTCTTGT 
TTGGGCCAGG AGGCTCTGAT TGAGCCAGTG CGGGACATGG GGCACTCTGA GATCATCCAT 
GGCCATTATC AGGGGACTCA GCCGTTTGAG AAAGGACTCG GCTTCCCGCA CAGAACAACC 
AACATGCGCA CTTGGGAGGA CATGCGTCTG AGCGGACAGG GTGATGAGGC TGAGGATCTT 
CCAGGAGAGC AAGTGAGCGA GGAGCAATTC ACAGACGAGC ATGGCAACAT CGTCACCAAA 
AAGCCGGATG TTGTGGATGT GAAGAGAGGA GGTGCTCAGA TAGTCAAATG TGCGAGTCTG 
CGGAGAGTAA AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331787.1
CDS	227..6184
Translation	MAQAAKHLRKNKDLEALAEQERKEKEEEKSKKRSRSREKKRKAHVVHRWLIDQDNSVSSELPDGQGVWHYDETADAATSFLRAARSGNLEKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLINHGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGLTPLHVSSFMGHLNIVKILMQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGANPNAAGKVGLTPLHVAVHHNNLDVVNLLLSKGGSPHSAARNGYTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPLHLASQEGQPDMVLLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKHGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSNGTSALAIAKRLGYISVIDVLKLVTEETVTMTTTEKHRMSFPETVDEILDVSEDEGIAQLTLGEELLGNEGARYLMKMEDIKDHDEDFLSPKKVLEYEGELENHSPAIPRIPCVSPETVMLKEHEMEQQHTPLPLPKEYDEDSLIPSSPATETSDNVSPVASPIHTGFLVSFMVDARGGSMRGSRHNGLRVIIPPRTCAAPTRITCRLVKPQKLPTPPPLVEGEGLASRIISLGPASMQFLGPVIVEIPHFAALGRGDRELVVLRSENGSVWKEHRNRYGDEVLETILNGMDEDLESQEELGKKRIRRIISTDFPLYFAVVSRIQQENDLIGPEGGFLSSKLVPMVQATFPETAVTKRVRLGLQAQPVPDELVAKLLGNQATFSPVVTVEPRRRKFHRPIGLRIPLPPSWRESPRDAGEGDTTSLRLLCSVIGGTAPAQWEDITGTTKLAYSNDCASFTTNVSARFWLADCPRTAEAVSFANLLYRELSAVPYMAKFVVFAKMNEAREGRLRCYCMTDDKMDKTLEQHENFSEVARSRDIEVMEGMPLYLECSGNLVPIRKAAQQPRCFSFQAFKDNRLPVSVKVRDSSKDPSGFLSFLRKSTKYEDSQHVLCNLNVTMPPCIKIMGSDERRRTLTPLALRERYSALNEPAMASLSAMERTELKMALIAEQLGLSWAELGRELQFNVDEINKIRVENPNSLLEQSSTLLNLWAAREGKRAKMDSLYVALKSIDRVDIVNMMEGQGGPPAGQQGGWEQDASRSRHHDRDHLSPGITNGYGLVVQEELVSPASMQYSLPSPLGNEPYWQEVSSMECAPIATTEEDTLMEMSEVQVWPSGNSPSIVAVEDSSLECSNADDSEALQNRSLGHSRSGTPGGNGLTPGGLSGSIELLEDQSIGADSDYSAAGMLRLSEGVNISALDRGQRSESSEGAVGHQVTNNIKDGSNGGLGEGGGDGTQLEDSISLISGQQRVYARMSESPGLRRRADPGEDRLSAGSFLSYLQDQGSPGWHSAAEATQNRLASLQCSLPRQAIESMMSSVRATVDSDSKQSCLGQEALIEPVRDMGHSEIIHGHYQGTQPFEKGLGFPHRTTNMRTWEDMRLSGQGDEAEDLPGEQVSEEQFTDEHGNIVTKKPDVVDVKRGGAQIVKCASLRRVKQ

Target ORF information:

RefSeq Version	XM_021476112.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476112.1

ORF Insert Sequence:

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGAATTGC ACAGCTAACA TTAGGCGAGG AGCTGCTGGG AAACGAGGGC 
GCCAGGTATT TGATGAAGAT GGAGGACATA AAGGACCATG ATGAGGATTT CCTTTCCCCC 
AAGAAAGTTC TAGAGTACGA GGGAGAGTTG GAGAATCATT CTCCTGCTAT CCCGAGGATC 
CCGTGTGTGT CTCCTGAGAC TGTCATGCTG AAAGAGCATG AGATGGAGCA GCAGCACACT 
CCACTTCCAT TGCCCAAAGA GTACGACGAG GATTCTCTGA TTCCCAGCAG TCCTGCAACT 
GAGACTTCAG ACAATGTCAG TCCGGTTGCC AGTCCCATCC ACACCGGTTT TCTGGTGAGC 
TTCATGGTGG ATGCTCGCGG CGGCTCGATG CGCGGCAGCA GACACAACGG CCTCCGCGTC 
ATCATTCCTC CTCGAACCTG CGCCGCTCCC ACACGCATCA CCTGCCGCCT GGTGAAGCCC 
CAGAAGCTGC CCACACCGCC GCCATTAGTG GAGGGAGAGG GTCTCGCCAG CCGCATCATC 
TCCCTTGGAC CCGCCAGCAT GCAGTTTTTA GGGCCTGTGA TTGTGGAGAT TCCTCATTTT 
GCGGCTCTCG GTCGGGGAGA TCGGGAACTC GTGGTCTTGA GGAGTGAGAA CGGATCGGTC 
TGGAAGGAAC ATCGTAACCG ATATGGAGAT GAAGTGCTAG AGACCATCCT CAACGGCATG 
GATGAAGATC TGGAGAGTCA GGAGGAGCTC GGGAAGAAAC GCATCAGACG GATCATCTCC 
ACCGACTTCC CGCTCTACTT TGCGGTGGTG TCTCGCATCC AGCAGGAGAA CGATCTGATT 
GGCCCAGAAG GAGGTTTTCT GTCCAGTAAA CTGGTGCCGA TGGTCCAGGC CACCTTTCCA 
GAGACAGCCG TCACCAAACG CGTGCGTCTG GGACTGCAGG CCCAGCCGGT GCCTGATGAG 
CTGGTGGCCA AGCTTTTGGG GAACCAGGCC ACGTTCAGTC CTGTGGTGAC GGTGGAGCCT 
CGGCGCCGGA AGTTTCACCG TCCCATCGGC CTAAGAATCC CACTGCCACC CTCATGGAGA 
GAGAGCCCGC GGGACGCCGG AGAGGGAGAC ACCACCAGCC TGCGCCTGCT CTGCAGCGTC 
ATCGGTGGAA CAGCTCCTGC CCAGTGGGAA GATATAACTG GCACCACCAA ACTGGCGTAT 
TCCAATGACT GTGCCAGTTT CACCACCAAC GTCTCTGCCC GATTCTGGCT GGCGGACTGT 
CCTCGGACTG CGGAGGCCGT GTCCTTTGCC AACCTCCTGT ACCGCGAGCT GTCGGCCGTG 
CCGTACATGG CCAAGTTTGT AGTGTTTGCG AAGATGAATG AAGCTCGCGA GGGTCGACTC 
CGCTGCTACT GCATGACGGA TGACAAGATG GACAAAACCC TGGAGCAGCA CGAGAACTTC 
TCGGAAGTGG CTCGCAGCAG AGACATCGAG GTGATGGAGG GAATGCCGCT GTATTTGGAG 
TGTTCAGGAA ACTTGGTTCC TATAAGAAAA GCAGCCCAGC AGCCTCGCTG TTTCAGCTTC 
CAGGCCTTTA AAGACAACAG ACTGCCTGTG TCCGTCAAGG TGAGGGACAG CAGTAAAGAC 
CCCTCTGGGT TTTTGTCTTT CTTGCGCAAG TCAACCAAAT ATGAAGACAG CCAACACGTG 
CTGTGCAACC TCAACGTGAC CATGCCGCCG TGCATTAAGA TTATGGGCAG CGATGAGCGG 
AGGAGGACTC TGACTCCTCT GGCTCTCAGA GAAAGATACA GCGCTCTGAA TGAACCGGCA 
ATGGCATCAC TGAGCGCCAT GGAAAGGACG GAGTTAAAGA TGGCTTTAAT AGCTGAACAG 
CTAGGCCTGA GCTGGGCTGA GTTGGGACGG GAACTGCAGT TTAATGTAGA TGAAATAAAT 
AAGATCCGTG TGGAGAATCC CAACTCCCTT TTAGAGCAGA GCTCAACGCT GCTCAACCTG 
TGGGCCGCCC GAGAGGGGAA GAGAGCCAAA ATGGACAGCC TGTACGTGGC CCTCAAGAGC 
ATCGACCGAG TGGACATTGT GAACATGATG GAGGGCCAGG GAGGACCACC GGCGGGTCAG 
CAGGGGGGCT GGGAGCAGGA CGCTTCTAGG TCTAGGCATC ACGACCGAGA CCATCTCTCA 
CCTGGAATCA CAAATGGTTA CGGGCTGGTG GTGCAGGAGG AACTAGTGTC ACCGGCCTCC 
ATGCAGTACA GTCTGCCCTC GCCACTGGGC AATGAGCCCT ACTGGCAGGA GGTTTCCAGC 
ATGGAGTGCG CGCCCATCGC CACCACTGAA GAAGACACGC TCATGGAGAT GTCCGAGGTA 
CAGGTTTGGC CCTCAGGAAA TAGTCCGTCC ATTGTGGCTG TAGAGGACTC CTCATTGGAG 
TGCAGCAATG CGGACGACTC CGAGGCGCTG CAGAACCGGA GCCTGGGTCA CTCCAGGAGC 
GGGACGCCCG GTGGGAACGG ACTCACTCCG GGAGGTTTAA GTGGCTCCAT CGAGCTCCTG 
GAGGATCAGT CCATCGGTGC GGATTCAGAC TACAGTGCCG CCGGGATGCT GCGTCTGAGC 
GAAGGGGTCA ACATCAGTGC TCTGGACAGA GGTCAGAGGT CGGAGAGTTC AGAAGGGGCT 
GTAGGTCACC AAGTCACCAA CAACATTAAA GACGGTTCCA ATGGAGGTTT GGGAGAAGGA 
GGAGGTGACG GCACACAGTT AGAAGATAGC ATATCTCTCA TTTCAGGGCA ACAGCGTGTG 
TATGCTCGAA TGAGCGAGTC GCCTGGGCTG AGAAGACGGG CAGATCCTGG CGAAGACAGG 
TTAAGCGCAG GGTCGTTCCT GTCTTATCTG CAGGATCAGG GCTCTCCTGG TTGGCATTCG 
GCTGCAGAAG CGACTCAGAA CAGATTGGCG TCTTTGCAAT GCTCCCTTCC TAGGCAGGCA 
ATAGAGTCTA TGATGTCATC GGTGCGAGCT ACGGTGGACT CTGACTCCAA GCAGTCTTGT 
TTGGGCCAGG AGGCTCTGAT TGAGCCAGTG CGGGACATGG GGCACTCTGA GATCATCCAT 
GGCCATTATC AGGGGACTCA GCCGTTTGAG AAAGGACTCG GCTTCCCGCA CAGAACAACC 
AACATGCGCA CTTGGGAGGA CATGCGTCTG AGCGGACAGG GTGATGAGGC TGAGGATCTT 
CCAGGAGAGC AAGTGAGCGA GGAGCAATTC ACAGACGAGC ATGGCAACAT CGTCACCAAA 
AAGCCGGATG TTGTGGATGT GAAGAGAGGA GGTGCTCAGA TAGTCAAATG TGCGAGTCTG 
CGGAGAGTAA AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_021476113.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331788.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X15, mRNA.

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CloneID	ODa142605
Clone ID Related Accession (Same CDS sequence)	XM_021476113.1
Accession Version	XM_021476113.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5874bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin 1, erythrocytic a isoform X15
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGAATTGC ACAGCTAACA TTAGGCGAGG AGCTGCTGGG AAACGAGGGC 
GCCAGGTATT TGATGAAGAT GGAGGACATA AAGGACCATG ATGAGGATTT CCTTTCCCCC 
AAGAAAGTTC TAGAGTACGA GGGAGAGTTG GAGAATCATT CTCCTGCTAT CCCGAGGATC 
CCGTGTGTGT CTCCTGAGAC TGTCATGCTG AAAGAGCATG AGATGGAGCA GCAGCACACT 
CCACTTCCAT TGCCCAAAGA GTACGACGAG GATTCTCTGA TTCCCAGCAG TCCTGCAACT 
GAGACTTCAG ACAATGTCAG TCCGGTTGCC AGTCCCATCC ACACCGGTTT TCTGGTGAGC 
TTCATGGTGG ATGCTCGCGG CGGCTCGATG CGCGGCAGCA GACACAACGG CCTCCGCGTC 
ATCATTCCTC CTCGAACCTG CGCCGCTCCC ACACGCATCA CCTGCCGCCT GGTGAAGCCC 
CAGAAGCTGC CCACACCGCC GCCATTAGTG GAGGGAGAGG GTCTCGCCAG CCGCATCATC 
TCCCTTGGAC CCGCCAGCAT GCAGTTTTTA GGGCCTGTGA TTGTGGAGAT TCCTCATTTT 
GCGGCTCTCG GTCGGGGAGA TCGGGAACTC GTGGTCTTGA GGAGTGAGAA CGGATCGGTC 
TGGAAGGAAC ATCGTAACCG ATATGGAGAT GAAGTGCTAG AGACCATCCT CAACGGCATG 
GATGAAGATC TGGAGAGTCA GGAGGAGCTC GGGAAGAAAC GCATCAGACG GATCATCTCC 
ACCGACTTCC CGCTCTACTT TGCGGTGGTG TCTCGCATCC AGCAGGAGAA CGATCTGATT 
GGCCCAGAAG GAGGTTTTCT GTCCAGTAAA CTGGTGCCGA TGGTCCAGGC CACCTTTCCA 
GAGACAGCCG TCACCAAACG CGTGCGTCTG GGACTGCAGG CCCAGCCGGT GCCTGATGAG 
CTGGTGGCCA AGCTTTTGGG GAACCAGGCC ACGTTCAGTC CTGTGGTGAC GGTGGAGCCT 
CGGCGCCGGA AGTTTCACCG TCCCATCGGC CTAAGAATCC CACTGCCACC CTCATGGAGA 
GAGAGCCCGC GGGACGCCGG AGAGGGAGAC ACCACCAGCC TGCGCCTGCT CTGCAGCGTC 
ATCGGTGGAA CAGCTCCTGC CCAGTGGGAA GATATAACTG GCACCACCAA ACTGGCGTAT 
TCCAATGACT GTGCCAGTTT CACCACCAAC GTCTCTGCCC GATTCTGGCT GGCGGACTGT 
CCTCGGACTG CGGAGGCCGT GTCCTTTGCC AACCTCCTGT ACCGCGAGCT GTCGGCCGTG 
CCGTACATGG CCAAGTTTGT AGTGTTTGCG AAGATGAATG AAGCTCGCGA GGGTCGACTC 
CGCTGCTACT GCATGACGGA TGACAAGATG GACAAAACCC TGGAGCAGCA CGAGAACTTC 
TCGGAAGTGG CTCGCAGCAG AGACATCGAG GTGATGGAGG GAATGCCGCT GTATTTGGAG 
TGTTCAGGAA ACTTGGTTCC TATAAGAAAA GCAGCCCAGC AGCCTCGCTG TTTCAGCTTC 
CAGGCCTTTA AAGACAACAG ACTGCCTGTG TCCGTCAAGG TGAGGGACAG CAGTAAAGAC 
CCCTCTGGGT TTTTGTCTTT CTTGCGCAAG TCAACCAAAT ATGAAGACAG CCAACACGTG 
CTGTGCAACC TCAACGTGAC CATGCCGCCG TGCATTAAGA TTATGGGCAG CGATGAGCGG 
AGGAGGACTC TGACTCCTCT GGCTCTCAGA GAAAGATACA GCGCTCTGAA TGAACCGGCA 
ATGGCATCAC TGAGCGCCAT GGAAAGGACG GAGTTAAAGA TGGCTTTAAT AGCTGAACAG 
CTAGGCCTGA GCTGGGCTGA GTTGGGACGG GAACTGCAGT TTAATGTAGA TGAAATAAAT 
AAGATCCGTG TGGAGAATCC CAACTCCCTT TTAGAGCAGA GCTCAACGCT GCTCAACCTG 
TGGGCCGCCC GAGAGGGGAA GAGAGCCAAA ATGGACAGCC TGTACGTGGC CCTCAAGAGC 
ATCGACCGAG TGGACATTGT GAACATGATG GAGGGCCAGG GAGGACCACC GGCGGGTCAG 
CAGGGGGGCT GGGAGCAGGA CGCTTCTAGG TCTAGGCATC ACGACCGAGA CCATCTCTCA 
CCTGGAATCA CAAATGGTTA CGGGCTGGTG GTGCAGGAGG AACTAGTGTC ACCGGCCTCC 
ATGCAGTACA GTCTGCCCTC GCCACTGGGC AATGAGCCCT ACTGGCAGGA GGTTTCCAGC 
ATGGAGTGCG CGCCCATCGC CACCACTGAA GAAGACACGC TCATGGAGAT GTCCGAGGTA 
CAGGTTTGGC CCTCAGGAAA TAGTCCGTCC ATTGTGGCTG TAGAGGACTC CTCATTGGAG 
TGCAGCAATG CGGACGACTC CGAGGCGCTG CAGAACCGGA GCCTGGGTCA CTCCAGGAGC 
GGGACGCCCG GTGGGAACGG ACTCACTCCG GGAGGTTTAA GTGGCTCCAT CGAGCTCCTG 
GAGGATCAGT CCATCGGTGC GGATTCAGAC TACAGTGCCG CCGGGATGCT GCGTCTGAGC 
GAAGGGGTCA ACATCAGTGC TCTGGACAGA GGTCAGAGGT CGGAGAGTTC AGAAGGGGCT 
GTAGGTCACC AAGTCACCAA CAACATTAAA GACGGTTCCA ATGGAGGTTT GGGAGAAGGA 
GGAGGTGACG GCACACAGTT AGAAGATAGC ATATCTCTCA TTTCAGGGCA ACAGCGTGTG 
TATGCTCGAA TGAGCGAGTC GCCTGGGCTG AGAAGACGGG CAGATCCTGG CGAAGACAGG 
TTAAGCGCAG GGTCGTTCCT GTCTTATCTG CAGGATCAGG GCTCTCCTGG TTGGCATTCG 
GCTGCAGAAG CGACTCAGAA CAGATTGGCG TCTTTGCAAT GCTCCCTTCC TAGGCAGGCA 
ATAGAGTCTA TGATGTCATC GGTGCGAGCT ACGGTGGACT CTGACTCCAA GCAGTCTTGT 
TTGGGCCAGG AGGCTCTGAT TGAGCCAGTG CGGGACATGG GGCACTCTGA GATCATCCAT 
GGCCATTATC AGGGGACTCA GCCGTTTGAG AAAGGACTCG GCTTCCCGCA CAGAACAACC 
AACATGCGCA CTTGGGAGGA CATGCGTCTG AGCGGACAGC CGGATGTTGT GGATGTGAAG 
AGAGGAGGTG CTCAGATAGT CAAATGTGCG AGTCTGCGGA GAGTAAAGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331788.1
CDS	228..6101
Translation	MAQAAKHLRKNKDLEALAEQERKEKEEEKSKKRSRSREKKRKAHVVHRWLIDQDNSVSSELPDGQGVWHYDETADAATSFLRAARSGNLEKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLINHGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGLTPLHVSSFMGHLNIVKILMQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGANPNAAGKVGLTPLHVAVHHNNLDVVNLLLSKGGSPHSAARNGYTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPLHLASQEGQPDMVLLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKHGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSNGTSALAIAKRLGYISVIDVLKLVTEETVTMTTTEKHRMSFPETVDEILDVSEDEGIAQLTLGEELLGNEGARYLMKMEDIKDHDEDFLSPKKVLEYEGELENHSPAIPRIPCVSPETVMLKEHEMEQQHTPLPLPKEYDEDSLIPSSPATETSDNVSPVASPIHTGFLVSFMVDARGGSMRGSRHNGLRVIIPPRTCAAPTRITCRLVKPQKLPTPPPLVEGEGLASRIISLGPASMQFLGPVIVEIPHFAALGRGDRELVVLRSENGSVWKEHRNRYGDEVLETILNGMDEDLESQEELGKKRIRRIISTDFPLYFAVVSRIQQENDLIGPEGGFLSSKLVPMVQATFPETAVTKRVRLGLQAQPVPDELVAKLLGNQATFSPVVTVEPRRRKFHRPIGLRIPLPPSWRESPRDAGEGDTTSLRLLCSVIGGTAPAQWEDITGTTKLAYSNDCASFTTNVSARFWLADCPRTAEAVSFANLLYRELSAVPYMAKFVVFAKMNEAREGRLRCYCMTDDKMDKTLEQHENFSEVARSRDIEVMEGMPLYLECSGNLVPIRKAAQQPRCFSFQAFKDNRLPVSVKVRDSSKDPSGFLSFLRKSTKYEDSQHVLCNLNVTMPPCIKIMGSDERRRTLTPLALRERYSALNEPAMASLSAMERTELKMALIAEQLGLSWAELGRELQFNVDEINKIRVENPNSLLEQSSTLLNLWAAREGKRAKMDSLYVALKSIDRVDIVNMMEGQGGPPAGQQGGWEQDASRSRHHDRDHLSPGITNGYGLVVQEELVSPASMQYSLPSPLGNEPYWQEVSSMECAPIATTEEDTLMEMSEVQVWPSGNSPSIVAVEDSSLECSNADDSEALQNRSLGHSRSGTPGGNGLTPGGLSGSIELLEDQSIGADSDYSAAGMLRLSEGVNISALDRGQRSESSEGAVGHQVTNNIKDGSNGGLGEGGGDGTQLEDSISLISGQQRVYARMSESPGLRRRADPGEDRLSAGSFLSYLQDQGSPGWHSAAEATQNRLASLQCSLPRQAIESMMSSVRATVDSDSKQSCLGQEALIEPVRDMGHSEIIHGHYQGTQPFEKGLGFPHRTTNMRTWEDMRLSGQPDVVDVKRGGAQIVKCASLRRVKQ

Target ORF information:

RefSeq Version	XM_021476113.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X15, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476113.1

ORF Insert Sequence:

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGAATTGC ACAGCTAACA TTAGGCGAGG AGCTGCTGGG AAACGAGGGC 
GCCAGGTATT TGATGAAGAT GGAGGACATA AAGGACCATG ATGAGGATTT CCTTTCCCCC 
AAGAAAGTTC TAGAGTACGA GGGAGAGTTG GAGAATCATT CTCCTGCTAT CCCGAGGATC 
CCGTGTGTGT CTCCTGAGAC TGTCATGCTG AAAGAGCATG AGATGGAGCA GCAGCACACT 
CCACTTCCAT TGCCCAAAGA GTACGACGAG GATTCTCTGA TTCCCAGCAG TCCTGCAACT 
GAGACTTCAG ACAATGTCAG TCCGGTTGCC AGTCCCATCC ACACCGGTTT TCTGGTGAGC 
TTCATGGTGG ATGCTCGCGG CGGCTCGATG CGCGGCAGCA GACACAACGG CCTCCGCGTC 
ATCATTCCTC CTCGAACCTG CGCCGCTCCC ACACGCATCA CCTGCCGCCT GGTGAAGCCC 
CAGAAGCTGC CCACACCGCC GCCATTAGTG GAGGGAGAGG GTCTCGCCAG CCGCATCATC 
TCCCTTGGAC CCGCCAGCAT GCAGTTTTTA GGGCCTGTGA TTGTGGAGAT TCCTCATTTT 
GCGGCTCTCG GTCGGGGAGA TCGGGAACTC GTGGTCTTGA GGAGTGAGAA CGGATCGGTC 
TGGAAGGAAC ATCGTAACCG ATATGGAGAT GAAGTGCTAG AGACCATCCT CAACGGCATG 
GATGAAGATC TGGAGAGTCA GGAGGAGCTC GGGAAGAAAC GCATCAGACG GATCATCTCC 
ACCGACTTCC CGCTCTACTT TGCGGTGGTG TCTCGCATCC AGCAGGAGAA CGATCTGATT 
GGCCCAGAAG GAGGTTTTCT GTCCAGTAAA CTGGTGCCGA TGGTCCAGGC CACCTTTCCA 
GAGACAGCCG TCACCAAACG CGTGCGTCTG GGACTGCAGG CCCAGCCGGT GCCTGATGAG 
CTGGTGGCCA AGCTTTTGGG GAACCAGGCC ACGTTCAGTC CTGTGGTGAC GGTGGAGCCT 
CGGCGCCGGA AGTTTCACCG TCCCATCGGC CTAAGAATCC CACTGCCACC CTCATGGAGA 
GAGAGCCCGC GGGACGCCGG AGAGGGAGAC ACCACCAGCC TGCGCCTGCT CTGCAGCGTC 
ATCGGTGGAA CAGCTCCTGC CCAGTGGGAA GATATAACTG GCACCACCAA ACTGGCGTAT 
TCCAATGACT GTGCCAGTTT CACCACCAAC GTCTCTGCCC GATTCTGGCT GGCGGACTGT 
CCTCGGACTG CGGAGGCCGT GTCCTTTGCC AACCTCCTGT ACCGCGAGCT GTCGGCCGTG 
CCGTACATGG CCAAGTTTGT AGTGTTTGCG AAGATGAATG AAGCTCGCGA GGGTCGACTC 
CGCTGCTACT GCATGACGGA TGACAAGATG GACAAAACCC TGGAGCAGCA CGAGAACTTC 
TCGGAAGTGG CTCGCAGCAG AGACATCGAG GTGATGGAGG GAATGCCGCT GTATTTGGAG 
TGTTCAGGAA ACTTGGTTCC TATAAGAAAA GCAGCCCAGC AGCCTCGCTG TTTCAGCTTC 
CAGGCCTTTA AAGACAACAG ACTGCCTGTG TCCGTCAAGG TGAGGGACAG CAGTAAAGAC 
CCCTCTGGGT TTTTGTCTTT CTTGCGCAAG TCAACCAAAT ATGAAGACAG CCAACACGTG 
CTGTGCAACC TCAACGTGAC CATGCCGCCG TGCATTAAGA TTATGGGCAG CGATGAGCGG 
AGGAGGACTC TGACTCCTCT GGCTCTCAGA GAAAGATACA GCGCTCTGAA TGAACCGGCA 
ATGGCATCAC TGAGCGCCAT GGAAAGGACG GAGTTAAAGA TGGCTTTAAT AGCTGAACAG 
CTAGGCCTGA GCTGGGCTGA GTTGGGACGG GAACTGCAGT TTAATGTAGA TGAAATAAAT 
AAGATCCGTG TGGAGAATCC CAACTCCCTT TTAGAGCAGA GCTCAACGCT GCTCAACCTG 
TGGGCCGCCC GAGAGGGGAA GAGAGCCAAA ATGGACAGCC TGTACGTGGC CCTCAAGAGC 
ATCGACCGAG TGGACATTGT GAACATGATG GAGGGCCAGG GAGGACCACC GGCGGGTCAG 
CAGGGGGGCT GGGAGCAGGA CGCTTCTAGG TCTAGGCATC ACGACCGAGA CCATCTCTCA 
CCTGGAATCA CAAATGGTTA CGGGCTGGTG GTGCAGGAGG AACTAGTGTC ACCGGCCTCC 
ATGCAGTACA GTCTGCCCTC GCCACTGGGC AATGAGCCCT ACTGGCAGGA GGTTTCCAGC 
ATGGAGTGCG CGCCCATCGC CACCACTGAA GAAGACACGC TCATGGAGAT GTCCGAGGTA 
CAGGTTTGGC CCTCAGGAAA TAGTCCGTCC ATTGTGGCTG TAGAGGACTC CTCATTGGAG 
TGCAGCAATG CGGACGACTC CGAGGCGCTG CAGAACCGGA GCCTGGGTCA CTCCAGGAGC 
GGGACGCCCG GTGGGAACGG ACTCACTCCG GGAGGTTTAA GTGGCTCCAT CGAGCTCCTG 
GAGGATCAGT CCATCGGTGC GGATTCAGAC TACAGTGCCG CCGGGATGCT GCGTCTGAGC 
GAAGGGGTCA ACATCAGTGC TCTGGACAGA GGTCAGAGGT CGGAGAGTTC AGAAGGGGCT 
GTAGGTCACC AAGTCACCAA CAACATTAAA GACGGTTCCA ATGGAGGTTT GGGAGAAGGA 
GGAGGTGACG GCACACAGTT AGAAGATAGC ATATCTCTCA TTTCAGGGCA ACAGCGTGTG 
TATGCTCGAA TGAGCGAGTC GCCTGGGCTG AGAAGACGGG CAGATCCTGG CGAAGACAGG 
TTAAGCGCAG GGTCGTTCCT GTCTTATCTG CAGGATCAGG GCTCTCCTGG TTGGCATTCG 
GCTGCAGAAG CGACTCAGAA CAGATTGGCG TCTTTGCAAT GCTCCCTTCC TAGGCAGGCA 
ATAGAGTCTA TGATGTCATC GGTGCGAGCT ACGGTGGACT CTGACTCCAA GCAGTCTTGT 
TTGGGCCAGG AGGCTCTGAT TGAGCCAGTG CGGGACATGG GGCACTCTGA GATCATCCAT 
GGCCATTATC AGGGGACTCA GCCGTTTGAG AAAGGACTCG GCTTCCCGCA CAGAACAACC 
AACATGCGCA CTTGGGAGGA CATGCGTCTG AGCGGACAGC CGGATGTTGT GGATGTGAAG 
AGAGGAGGTG CTCAGATAGT CAAATGTGCG AGTCTGCGGA GAGTAAAGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_021476114.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331789.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X16, mRNA.

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CloneID	ODa142606
Clone ID Related Accession (Same CDS sequence)	XM_021476114.1
Accession Version	XM_021476114.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5532bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin 1, erythrocytic a isoform X16
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGAATTGC ACAGCTAACA TTAGGCGAGG AGCTGCTGGG AAACGAGGGC 
GCCAGGTATT TGATGAAGAT GGAGGACATA AAGGACCATG ATGAGGATTT CCTTTCCCCC 
AAGAAAGTTC TAGAGTACGA GGGAGAGTTG GAGAATCATT CTCCTGCTAT CCCGAGGATC 
CCGTGTGTGT CTCCTGAGAC TGTCATGCTG AAAGAGCATG AGATGGAGCA GCAGCACACT 
CCACTTCCAT TGCCCAAAGA GTACGACGAG GATTCTCTGA TTCCCAGCAG TCCTGCAACT 
GAGACTTCAG ACAATGTCAG TCCGGTTGCC AGTCCCATCC ACACCGGTTT TCTGGTGAGC 
TTCATGGTGG ATGCTCGCGG CGGCTCGATG CGCGGCAGCA GACACAACGG CCTCCGCGTC 
ATCATTCCTC CTCGAACCTG CGCCGCTCCC ACACGCATCA CCTGCCGCCT GGTGAAGCCC 
CAGAAGCTGC CCACACCGCC GCCATTAGTG GAGGGAGAGG GTCTCGCCAG CCGCATCATC 
TCCCTTGGAC CCGCCAGCAT GCAGTTTTTA GGGCCTGTGA TTGTGGAGAT TCCTCATTTT 
GCGGCTCTCG GTCGGGGAGA TCGGGAACTC GTGGTCTTGA GGAGTGAGAA CGGATCGGTC 
TGGAAGGAAC ATCGTAACCG ATATGGAGAT GAAGTGCTAG AGACCATCCT CAACGGCATG 
GATGAAGATC TGGAGAGTCA GGAGGAGCTC GGGAAGAAAC GCATCAGACG GATCATCTCC 
ACCGACTTCC CGCTCTACTT TGCGGTGGTG TCTCGCATCC AGCAGGAGAA CGATCTGATT 
GGCCCAGAAG GAGGTTTTCT GTCCAGTAAA CTGGTGCCGA TGGTCCAGGC CACCTTTCCA 
GAGACAGCCG TCACCAAACG CGTGCGTCTG GGACTGCAGG CCCAGCCGGT GCCTGATGAG 
CTGGTGGCCA AGCTTTTGGG GAACCAGGCC ACGTTCAGTC CTGTGGTGAC GGTGGAGCCT 
CGGCGCCGGA AGTTTCACCG TCCCATCGGC CTAAGAATCC CACTGCCACC CTCATGGAGA 
GAGAGCCCGC GGGACGCCGG AGAGGGAGAC ACCACCAGCC TGCGCCTGCT CTGCAGCGTC 
ATCGGTGGAA CAGCTCCTGC CCAGTGGGAA GATATAACTG GCACCACCAA ACTGGCGTAT 
TCCAATGACT GTGCCAGTTT CACCACCAAC GTCTCTGCCC GATTCTGGCT GGCGGACTGT 
CCTCGGACTG CGGAGGCCGT GTCCTTTGCC AACCTCCTGT ACCGCGAGCT GTCGGCCGTG 
CCGTACATGG CCAAGTTTGT AGTGTTTGCG AAGATGAATG AAGCTCGCGA GGGTCGACTC 
CGCTGCTACT GCATGACGGA TGACAAGATG GACAAAACCC TGGAGCAGCA CGAGAACTTC 
TCGGAAGTGG CTCGCAGCAG AGACATCGAG GTGATGGAGG GAATGCCGCT GTATTTGGAG 
TGTTCAGGAA ACTTGGTTCC TATAAGAAAA GCAGCCCAGC AGCCTCGCTG TTTCAGCTTC 
CAGGCCTTTA AAGACAACAG ACTGCCTGTG TCCGTCAAGG TGAGGGACAG CAGTAAAGAC 
CCCTCTGGGT TTTTGTCTTT CTTGCGCAAG TCAACCAAAT ATGAAGACAG CCAACACGTG 
CTGTGCAACC TCAACGTGAC CATGCCGCCG TGCATTAAGA TTATGGGCAG CGATGAGCGG 
AGGAGGACTC TGACTCCTCT GGCTCTCAGA GAAAGATACA GCGCTCTGAA TGAACCGGCA 
ATGGCATCAC TGAGCGCCAT GGAAAGGACG GAGTTAAAGA TGGCTTTAAT AGCTGAACAG 
CTAGGCCTGA GCTGGGCTGA GTTGGGACGG GAACTGCAGT TTAATGTAGA TGAAATAAAT 
AAGATCCGTG TGGAGAATCC CAACTCCCTT TTAGAGCAGA GCTCAACGCT GCTCAACCTG 
TGGGCCGCCC GAGAGGGGAA GAGAGCCAAA ATGGACAGCC TGTACGTGGC CCTCAAGAGC 
ATCGACCGAG TGGACATTGT GAACATGATG GAGGGCCAGG GAGGACCACC GGCGGGTCAG 
CAGGGGGGCT GGGAGCAGGA CGCTTCTAGG TCTAGGCATC ACGACCGAGA CCATCTCTCA 
CCTGGAATCA CAAATGGGCA ACAGCGTGTG TATGCTCGAA TGAGCGAGTC GCCTGGGCTG 
AGAAGACGGG CAGATCCTGG CGAAGACAGG TTAAGCGCAG GGTCGTTCCT GTCTTATCTG 
CAGGATCAGG GCTCTCCTGG TTGGCATTCG GCTGCAGAAG CGACTCAGAA CAGATTGGCG 
TCTTTGCAAT GCTCCCTTCC TAGGCAGGCA ATAGAGTCTA TGATGTCATC GGTGCGAGCT 
ACGGTGGACT CTGACTCCAA GCAGTCTTGT TTGGGCCAGG AGGCTCTGAT TGAGCCAGTG 
CGGGACATGG GGCACTCTGA GATCATCCAT GGCCATTATC AGGGGACTCA GCCGTTTGAG 
AAAGGACTCG GCTTCCCGCA CAGAACAACC AACATGCGCA CTTGGGAGGA CATGCGTCTG 
AGCGGACAGG GTGATGAGGC TGAGGATCTT CCAGGAGAGC AAGTGAGCGA GGAGCAATTC 
ACAGACGAGC ATGGCAACAT CGTCACCAAA AAGATTGTGA GGAAAGTGGT CCGGAGAGGA 
AAAGGGGAGG AAGGGGGTCA GGAGCTGATC ATTGAAGGTC TGCAGCAGGA TATCAGTGAG 
CCTGACTTTG ATGGAGAACA GTTCATGAGC TATGCCGTCC TGGGACGTGA CAGCAAGCCG 
GATGTTGTGG ATGTGAAGAG AGGAGGTGCT CAGATAGTCA AATGTGCGAG TCTGCGGAGA 
GTAAAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331789.1
CDS	220..5751
Translation	MAQAAKHLRKNKDLEALAEQERKEKEEEKSKKRSRSREKKRKAHVVHRWLIDQDNSVSSELPDGQGVWHYDETADAATSFLRAARSGNLEKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLINHGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGLTPLHVSSFMGHLNIVKILMQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGANPNAAGKVGLTPLHVAVHHNNLDVVNLLLSKGGSPHSAARNGYTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPLHLASQEGQPDMVLLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKHGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSNGTSALAIAKRLGYISVIDVLKLVTEETVTMTTTEKHRMSFPETVDEILDVSEDEGIAQLTLGEELLGNEGARYLMKMEDIKDHDEDFLSPKKVLEYEGELENHSPAIPRIPCVSPETVMLKEHEMEQQHTPLPLPKEYDEDSLIPSSPATETSDNVSPVASPIHTGFLVSFMVDARGGSMRGSRHNGLRVIIPPRTCAAPTRITCRLVKPQKLPTPPPLVEGEGLASRIISLGPASMQFLGPVIVEIPHFAALGRGDRELVVLRSENGSVWKEHRNRYGDEVLETILNGMDEDLESQEELGKKRIRRIISTDFPLYFAVVSRIQQENDLIGPEGGFLSSKLVPMVQATFPETAVTKRVRLGLQAQPVPDELVAKLLGNQATFSPVVTVEPRRRKFHRPIGLRIPLPPSWRESPRDAGEGDTTSLRLLCSVIGGTAPAQWEDITGTTKLAYSNDCASFTTNVSARFWLADCPRTAEAVSFANLLYRELSAVPYMAKFVVFAKMNEAREGRLRCYCMTDDKMDKTLEQHENFSEVARSRDIEVMEGMPLYLECSGNLVPIRKAAQQPRCFSFQAFKDNRLPVSVKVRDSSKDPSGFLSFLRKSTKYEDSQHVLCNLNVTMPPCIKIMGSDERRRTLTPLALRERYSALNEPAMASLSAMERTELKMALIAEQLGLSWAELGRELQFNVDEINKIRVENPNSLLEQSSTLLNLWAAREGKRAKMDSLYVALKSIDRVDIVNMMEGQGGPPAGQQGGWEQDASRSRHHDRDHLSPGITNGQQRVYARMSESPGLRRRADPGEDRLSAGSFLSYLQDQGSPGWHSAAEATQNRLASLQCSLPRQAIESMMSSVRATVDSDSKQSCLGQEALIEPVRDMGHSEIIHGHYQGTQPFEKGLGFPHRTTNMRTWEDMRLSGQGDEAEDLPGEQVSEEQFTDEHGNIVTKKIVRKVVRRGKGEEGGQELIIEGLQQDISEPDFDGEQFMSYAVLGRDSKPDVVDVKRGGAQIVKCASLRRVKQ

Target ORF information:

RefSeq Version	XM_021476114.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X16, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476114.1

ORF Insert Sequence:

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGAATTGC ACAGCTAACA TTAGGCGAGG AGCTGCTGGG AAACGAGGGC 
GCCAGGTATT TGATGAAGAT GGAGGACATA AAGGACCATG ATGAGGATTT CCTTTCCCCC 
AAGAAAGTTC TAGAGTACGA GGGAGAGTTG GAGAATCATT CTCCTGCTAT CCCGAGGATC 
CCGTGTGTGT CTCCTGAGAC TGTCATGCTG AAAGAGCATG AGATGGAGCA GCAGCACACT 
CCACTTCCAT TGCCCAAAGA GTACGACGAG GATTCTCTGA TTCCCAGCAG TCCTGCAACT 
GAGACTTCAG ACAATGTCAG TCCGGTTGCC AGTCCCATCC ACACCGGTTT TCTGGTGAGC 
TTCATGGTGG ATGCTCGCGG CGGCTCGATG CGCGGCAGCA GACACAACGG CCTCCGCGTC 
ATCATTCCTC CTCGAACCTG CGCCGCTCCC ACACGCATCA CCTGCCGCCT GGTGAAGCCC 
CAGAAGCTGC CCACACCGCC GCCATTAGTG GAGGGAGAGG GTCTCGCCAG CCGCATCATC 
TCCCTTGGAC CCGCCAGCAT GCAGTTTTTA GGGCCTGTGA TTGTGGAGAT TCCTCATTTT 
GCGGCTCTCG GTCGGGGAGA TCGGGAACTC GTGGTCTTGA GGAGTGAGAA CGGATCGGTC 
TGGAAGGAAC ATCGTAACCG ATATGGAGAT GAAGTGCTAG AGACCATCCT CAACGGCATG 
GATGAAGATC TGGAGAGTCA GGAGGAGCTC GGGAAGAAAC GCATCAGACG GATCATCTCC 
ACCGACTTCC CGCTCTACTT TGCGGTGGTG TCTCGCATCC AGCAGGAGAA CGATCTGATT 
GGCCCAGAAG GAGGTTTTCT GTCCAGTAAA CTGGTGCCGA TGGTCCAGGC CACCTTTCCA 
GAGACAGCCG TCACCAAACG CGTGCGTCTG GGACTGCAGG CCCAGCCGGT GCCTGATGAG 
CTGGTGGCCA AGCTTTTGGG GAACCAGGCC ACGTTCAGTC CTGTGGTGAC GGTGGAGCCT 
CGGCGCCGGA AGTTTCACCG TCCCATCGGC CTAAGAATCC CACTGCCACC CTCATGGAGA 
GAGAGCCCGC GGGACGCCGG AGAGGGAGAC ACCACCAGCC TGCGCCTGCT CTGCAGCGTC 
ATCGGTGGAA CAGCTCCTGC CCAGTGGGAA GATATAACTG GCACCACCAA ACTGGCGTAT 
TCCAATGACT GTGCCAGTTT CACCACCAAC GTCTCTGCCC GATTCTGGCT GGCGGACTGT 
CCTCGGACTG CGGAGGCCGT GTCCTTTGCC AACCTCCTGT ACCGCGAGCT GTCGGCCGTG 
CCGTACATGG CCAAGTTTGT AGTGTTTGCG AAGATGAATG AAGCTCGCGA GGGTCGACTC 
CGCTGCTACT GCATGACGGA TGACAAGATG GACAAAACCC TGGAGCAGCA CGAGAACTTC 
TCGGAAGTGG CTCGCAGCAG AGACATCGAG GTGATGGAGG GAATGCCGCT GTATTTGGAG 
TGTTCAGGAA ACTTGGTTCC TATAAGAAAA GCAGCCCAGC AGCCTCGCTG TTTCAGCTTC 
CAGGCCTTTA AAGACAACAG ACTGCCTGTG TCCGTCAAGG TGAGGGACAG CAGTAAAGAC 
CCCTCTGGGT TTTTGTCTTT CTTGCGCAAG TCAACCAAAT ATGAAGACAG CCAACACGTG 
CTGTGCAACC TCAACGTGAC CATGCCGCCG TGCATTAAGA TTATGGGCAG CGATGAGCGG 
AGGAGGACTC TGACTCCTCT GGCTCTCAGA GAAAGATACA GCGCTCTGAA TGAACCGGCA 
ATGGCATCAC TGAGCGCCAT GGAAAGGACG GAGTTAAAGA TGGCTTTAAT AGCTGAACAG 
CTAGGCCTGA GCTGGGCTGA GTTGGGACGG GAACTGCAGT TTAATGTAGA TGAAATAAAT 
AAGATCCGTG TGGAGAATCC CAACTCCCTT TTAGAGCAGA GCTCAACGCT GCTCAACCTG 
TGGGCCGCCC GAGAGGGGAA GAGAGCCAAA ATGGACAGCC TGTACGTGGC CCTCAAGAGC 
ATCGACCGAG TGGACATTGT GAACATGATG GAGGGCCAGG GAGGACCACC GGCGGGTCAG 
CAGGGGGGCT GGGAGCAGGA CGCTTCTAGG TCTAGGCATC ACGACCGAGA CCATCTCTCA 
CCTGGAATCA CAAATGGGCA ACAGCGTGTG TATGCTCGAA TGAGCGAGTC GCCTGGGCTG 
AGAAGACGGG CAGATCCTGG CGAAGACAGG TTAAGCGCAG GGTCGTTCCT GTCTTATCTG 
CAGGATCAGG GCTCTCCTGG TTGGCATTCG GCTGCAGAAG CGACTCAGAA CAGATTGGCG 
TCTTTGCAAT GCTCCCTTCC TAGGCAGGCA ATAGAGTCTA TGATGTCATC GGTGCGAGCT 
ACGGTGGACT CTGACTCCAA GCAGTCTTGT TTGGGCCAGG AGGCTCTGAT TGAGCCAGTG 
CGGGACATGG GGCACTCTGA GATCATCCAT GGCCATTATC AGGGGACTCA GCCGTTTGAG 
AAAGGACTCG GCTTCCCGCA CAGAACAACC AACATGCGCA CTTGGGAGGA CATGCGTCTG 
AGCGGACAGG GTGATGAGGC TGAGGATCTT CCAGGAGAGC AAGTGAGCGA GGAGCAATTC 
ACAGACGAGC ATGGCAACAT CGTCACCAAA AAGATTGTGA GGAAAGTGGT CCGGAGAGGA 
AAAGGGGAGG AAGGGGGTCA GGAGCTGATC ATTGAAGGTC TGCAGCAGGA TATCAGTGAG 
CCTGACTTTG ATGGAGAACA GTTCATGAGC TATGCCGTCC TGGGACGTGA CAGCAAGCCG 
GATGTTGTGG ATGTGAAGAG AGGAGGTGCT CAGATAGTCA AATGTGCGAG TCTGCGGAGA 
GTAAAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_021476115.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331790.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X17, mRNA.

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CloneID	ODa142607
Clone ID Related Accession (Same CDS sequence)	XM_021476115.1
Accession Version	XM_021476115.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4893bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin 1, erythrocytic a isoform X17
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGAATTGC ACAGCTAACA TTAGGCGAGG AGCTGCTGGG AAACGAGGGC 
GCCAGGTATT TGATGAAGAT GGAGGACATA AAGGACCATG ATGAGGATTT CCTTTCCCCC 
AAGAAAGTTC TAGAGTACGA GGGAGAGTTG GAGAATCATT CTCCTGCTAT CCCGAGGATC 
CCGTGTGTGT CTCCTGAGAC TGTCATGCTG AAAGAGCATG AGATGGAGCA GCAGCACACT 
CCACTTCCAT TGCCCAAAGA GTACGACGAG GATTCTCTGA TTCCCAGCAG TCCTGCAACT 
GAGACTTCAG ACAATGTCAG TCCGGTTGCC AGTCCCATCC ACACCGGTTT TCTGGTGAGC 
TTCATGGTGG ATGCTCGCGG CGGCTCGATG CGCGGCAGCA GACACAACGG CCTCCGCGTC 
ATCATTCCTC CTCGAACCTG CGCCGCTCCC ACACGCATCA CCTGCCGCCT GGTGAAGCCC 
CAGAAGCTGC CCACACCGCC GCCATTAGTG GAGGGAGAGG GTCTCGCCAG CCGCATCATC 
TCCCTTGGAC CCGCCAGCAT GCAGTTTTTA GGGCCTGTGA TTGTGGAGAT TCCTCATTTT 
GCGGCTCTCG GTCGGGGAGA TCGGGAACTC GTGGTCTTGA GGAGTGAGAA CGGATCGGTC 
TGGAAGGAAC ATCGTAACCG ATATGGAGAT GAAGTGCTAG AGACCATCCT CAACGGCATG 
GATGAAGATC TGGAGAGTCA GGAGGAGCTC GGGAAGAAAC GCATCAGACG GATCATCTCC 
ACCGACTTCC CGCTCTACTT TGCGGTGGTG TCTCGCATCC AGCAGGAGAA CGATCTGATT 
GGCCCAGAAG GAGGTTTTCT GTCCAGTAAA CTGGTGCCGA TGGTCCAGGC CACCTTTCCA 
GAGACAGCCG TCACCAAACG CGTGCGTCTG GGACTGCAGG CCCAGCCGGT GCCTGATGAG 
CTGGTGGCCA AGCTTTTGGG GAACCAGGCC ACGTTCAGTC CTGTGGTGAC GGTGGAGCCT 
CGGCGCCGGA AGTTTCACCG TCCCATCGGC CTAAGAATCC CACTGCCACC CTCATGGAGA 
GAGAGCCCGC GGGACGCCGG AGAGGGAGAC ACCACCAGCC TGCGCCTGCT CTGCAGCGTC 
ATCGGTGGAA CAGCTCCTGC CCAGTGGGAA GATATAACTG GCACCACCAA ACTGGCGTAT 
TCCAATGACT GTGCCAGTTT CACCACCAAC GTCTCTGCCC GATTCTGGCT GGCGGACTGT 
CCTCGGACTG CGGAGGCCGT GTCCTTTGCC AACCTCCTGT ACCGCGAGCT GTCGGCCGTG 
CCGTACATGG CCAAGTTTGT AGTGTTTGCG AAGATGAATG AAGCTCGCGA GGGTCGACTC 
CGCTGCTACT GCATGACGGA TGACAAGATG GACAAAACCC TGGAGCAGCA CGAGAACTTC 
TCGGAAGTGG CTCGCAGCAG AGACATCGAG GTGATGGAGG GAATGCCGCT GTATTTGGAG 
TGTTCAGGAA ACTTGGTTCC TATAAGAAAA GCAGCCCAGC AGCCTCGCTG TTTCAGCTTC 
CAGGCCTTTA AAGACAACAG ACTGCCTGTG TCCGTCAAGG TGAGGGACAG CAGTAAAGAC 
CCCTCTGGGT TTTTGTCTTT CTTGCGCAAG TCAACCAAAT ATGAAGACAG CCAACACGTG 
CTGTGCAACC TCAACGTGAC CATGCCGCCG TGCATTAAGA TTATGGGCAG CGATGAGCGG 
AGGAGGACTC TGACTCCTCT GGCTCTCAGA GAAAGATACA GCGCTCTGAA TGAACCGGCA 
ATGGCATCAC TGAGCGCCAT GGAAAGGACG GAGTTAAAGA TGGCTTTAAT AGCTGAACAG 
CTAGGCCTGA GCTGGGCTGA GTTGGGACGG GAACTGCAGT TTAATGTAGA TGAAATAAAT 
AAGATCCGTG TGGAGAATCC CAACTCCCTT TTAGAGCAGA GCTCAACGCT GCTCAACCTG 
TGGGCCGCCC GAGAGGGGAA GAGAGCCAAA ATGGACAGCC TGTACGTGGC CCTCAAGAGC 
ATCGACCGAG TGGACATTGT GAACATGATG GAGGGCCAGG GAGGACCACC GGCGGGTCAG 
CAGGGGGGCT GGGAGCAGGA CGCTTCTAGG TCTAGGCATC ACGACCGAGA CCATCTCTCA 
CCTGGAATCA CAAATGGCCT GTTTACATGC TCTTGTGGCA CTTTCTTGGA TGTTCCTCAC 
ACCATAATCT CCGCATGCAG GGTCCAGAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331790.1
CDS	225..5117
Translation	MAQAAKHLRKNKDLEALAEQERKEKEEEKSKKRSRSREKKRKAHVVHRWLIDQDNSVSSELPDGQGVWHYDETADAATSFLRAARSGNLEKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLINHGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGLTPLHVSSFMGHLNIVKILMQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGANPNAAGKVGLTPLHVAVHHNNLDVVNLLLSKGGSPHSAARNGYTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPLHLASQEGQPDMVLLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKHGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSNGTSALAIAKRLGYISVIDVLKLVTEETVTMTTTEKHRMSFPETVDEILDVSEDEGIAQLTLGEELLGNEGARYLMKMEDIKDHDEDFLSPKKVLEYEGELENHSPAIPRIPCVSPETVMLKEHEMEQQHTPLPLPKEYDEDSLIPSSPATETSDNVSPVASPIHTGFLVSFMVDARGGSMRGSRHNGLRVIIPPRTCAAPTRITCRLVKPQKLPTPPPLVEGEGLASRIISLGPASMQFLGPVIVEIPHFAALGRGDRELVVLRSENGSVWKEHRNRYGDEVLETILNGMDEDLESQEELGKKRIRRIISTDFPLYFAVVSRIQQENDLIGPEGGFLSSKLVPMVQATFPETAVTKRVRLGLQAQPVPDELVAKLLGNQATFSPVVTVEPRRRKFHRPIGLRIPLPPSWRESPRDAGEGDTTSLRLLCSVIGGTAPAQWEDITGTTKLAYSNDCASFTTNVSARFWLADCPRTAEAVSFANLLYRELSAVPYMAKFVVFAKMNEAREGRLRCYCMTDDKMDKTLEQHENFSEVARSRDIEVMEGMPLYLECSGNLVPIRKAAQQPRCFSFQAFKDNRLPVSVKVRDSSKDPSGFLSFLRKSTKYEDSQHVLCNLNVTMPPCIKIMGSDERRRTLTPLALRERYSALNEPAMASLSAMERTELKMALIAEQLGLSWAELGRELQFNVDEINKIRVENPNSLLEQSSTLLNLWAAREGKRAKMDSLYVALKSIDRVDIVNMMEGQGGPPAGQQGGWEQDASRSRHHDRDHLSPGITNGLFTCSCGTFLDVPHTIISACRVQK

Target ORF information:

RefSeq Version	XM_021476115.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X17, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476115.1

ORF Insert Sequence:

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGAATTGC ACAGCTAACA TTAGGCGAGG AGCTGCTGGG AAACGAGGGC 
GCCAGGTATT TGATGAAGAT GGAGGACATA AAGGACCATG ATGAGGATTT CCTTTCCCCC 
AAGAAAGTTC TAGAGTACGA GGGAGAGTTG GAGAATCATT CTCCTGCTAT CCCGAGGATC 
CCGTGTGTGT CTCCTGAGAC TGTCATGCTG AAAGAGCATG AGATGGAGCA GCAGCACACT 
CCACTTCCAT TGCCCAAAGA GTACGACGAG GATTCTCTGA TTCCCAGCAG TCCTGCAACT 
GAGACTTCAG ACAATGTCAG TCCGGTTGCC AGTCCCATCC ACACCGGTTT TCTGGTGAGC 
TTCATGGTGG ATGCTCGCGG CGGCTCGATG CGCGGCAGCA GACACAACGG CCTCCGCGTC 
ATCATTCCTC CTCGAACCTG CGCCGCTCCC ACACGCATCA CCTGCCGCCT GGTGAAGCCC 
CAGAAGCTGC CCACACCGCC GCCATTAGTG GAGGGAGAGG GTCTCGCCAG CCGCATCATC 
TCCCTTGGAC CCGCCAGCAT GCAGTTTTTA GGGCCTGTGA TTGTGGAGAT TCCTCATTTT 
GCGGCTCTCG GTCGGGGAGA TCGGGAACTC GTGGTCTTGA GGAGTGAGAA CGGATCGGTC 
TGGAAGGAAC ATCGTAACCG ATATGGAGAT GAAGTGCTAG AGACCATCCT CAACGGCATG 
GATGAAGATC TGGAGAGTCA GGAGGAGCTC GGGAAGAAAC GCATCAGACG GATCATCTCC 
ACCGACTTCC CGCTCTACTT TGCGGTGGTG TCTCGCATCC AGCAGGAGAA CGATCTGATT 
GGCCCAGAAG GAGGTTTTCT GTCCAGTAAA CTGGTGCCGA TGGTCCAGGC CACCTTTCCA 
GAGACAGCCG TCACCAAACG CGTGCGTCTG GGACTGCAGG CCCAGCCGGT GCCTGATGAG 
CTGGTGGCCA AGCTTTTGGG GAACCAGGCC ACGTTCAGTC CTGTGGTGAC GGTGGAGCCT 
CGGCGCCGGA AGTTTCACCG TCCCATCGGC CTAAGAATCC CACTGCCACC CTCATGGAGA 
GAGAGCCCGC GGGACGCCGG AGAGGGAGAC ACCACCAGCC TGCGCCTGCT CTGCAGCGTC 
ATCGGTGGAA CAGCTCCTGC CCAGTGGGAA GATATAACTG GCACCACCAA ACTGGCGTAT 
TCCAATGACT GTGCCAGTTT CACCACCAAC GTCTCTGCCC GATTCTGGCT GGCGGACTGT 
CCTCGGACTG CGGAGGCCGT GTCCTTTGCC AACCTCCTGT ACCGCGAGCT GTCGGCCGTG 
CCGTACATGG CCAAGTTTGT AGTGTTTGCG AAGATGAATG AAGCTCGCGA GGGTCGACTC 
CGCTGCTACT GCATGACGGA TGACAAGATG GACAAAACCC TGGAGCAGCA CGAGAACTTC 
TCGGAAGTGG CTCGCAGCAG AGACATCGAG GTGATGGAGG GAATGCCGCT GTATTTGGAG 
TGTTCAGGAA ACTTGGTTCC TATAAGAAAA GCAGCCCAGC AGCCTCGCTG TTTCAGCTTC 
CAGGCCTTTA AAGACAACAG ACTGCCTGTG TCCGTCAAGG TGAGGGACAG CAGTAAAGAC 
CCCTCTGGGT TTTTGTCTTT CTTGCGCAAG TCAACCAAAT ATGAAGACAG CCAACACGTG 
CTGTGCAACC TCAACGTGAC CATGCCGCCG TGCATTAAGA TTATGGGCAG CGATGAGCGG 
AGGAGGACTC TGACTCCTCT GGCTCTCAGA GAAAGATACA GCGCTCTGAA TGAACCGGCA 
ATGGCATCAC TGAGCGCCAT GGAAAGGACG GAGTTAAAGA TGGCTTTAAT AGCTGAACAG 
CTAGGCCTGA GCTGGGCTGA GTTGGGACGG GAACTGCAGT TTAATGTAGA TGAAATAAAT 
AAGATCCGTG TGGAGAATCC CAACTCCCTT TTAGAGCAGA GCTCAACGCT GCTCAACCTG 
TGGGCCGCCC GAGAGGGGAA GAGAGCCAAA ATGGACAGCC TGTACGTGGC CCTCAAGAGC 
ATCGACCGAG TGGACATTGT GAACATGATG GAGGGCCAGG GAGGACCACC GGCGGGTCAG 
CAGGGGGGCT GGGAGCAGGA CGCTTCTAGG TCTAGGCATC ACGACCGAGA CCATCTCTCA 
CCTGGAATCA CAAATGGCCT GTTTACATGC TCTTGTGGCA CTTTCTTGGA TGTTCCTCAC 
ACCATAATCT CCGCATGCAG GGTCCAGAAG TAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_021476117.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331792.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X19, mRNA.

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CloneID	ODa142608
Clone ID Related Accession (Same CDS sequence)	XM_021476117.1 , XM_021476116.1
Accession Version	XM_021476117.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin 1, erythrocytic a isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGAATTGC ACAGCTAACA TTAGGCGAGG AGCTGCTGGG AAACGAGGGC 
GCCAGGTATT TGATGAAGAT GGAGGACATA AAGGACCATG ATGAGGATTT CCTTTCCCCC 
AAGAAAGTTC TAGAGTACGA GGGAGAGTTG GAGAATCATT CTCCTGCTAT CCCGAGGATC 
CCGTGTGTGT CTCCTGAGAC TGTCATGCTG AAAGAGCATG AGATGGAGCA GCAGCACACT 
CCACTTCCAT TGCCCAAAGA GTACGACGAG GATTCTCTGA TTCCCAGCAG TCCTGCAACT 
GAGACTTCAG ACAATGTCAG TCCGGTTGCC AGTCCCATCC ACACCGGTTT TCTGGTGAGC 
TTCATGGTGG ATGCTCGCGG CGGCTCGATG CGCGGCAGCA GACACAACGG CCTCCGCGTC 
ATCATTCCTC CTCGAACCTG CGCCGCTCCC ACACGCATCA CCTGCCGCCT GGTGAAGCCC 
CAGAAGCTGC CCACACCGCC GCCATTAGTG GAGGGAGAGG GTCTCGCCAG CCGCATCATC 
TCCCTTGGAC CCGCCAGCAT GCAGTTTTTA GGGCCTGTGA TTGTGGAGAT TCCTCATTTT 
GCGGCTCTCG GTCGGGGAGA TCGGGAACTC GTGGTCTTGA GGAGTGAGAA CGGATCGGTC 
TGGAAGGAAC ATCGTAACCG ATATGGAGAT GAAGTGCTAG AGACCATCCT CAACGGCATG 
GATGAAGATC TGGAGAGTCA GGAGGAGCTC GGGAAGAAAC GCATCAGACG GATCATCTCC 
ACCGACTTCC CGCTCTACTT TGCGGTGGTG TCTCGCATCC AGCAGGAGAA CGATCTGATT 
GGCCCAGAAG GAGGTTTTCT GTCCAGTAAA CTGGTGCCGA TGGTCCAGGC CACCTTTCCA 
GAGACAGCCG TCACCAAACG CGTGCGTCTG GGACTGCAGG CCCAGCCGGT GCCTGATGAG 
CTGGTGGCCA AGCTTTTGGG GAACCAGGCC ACGTTCAGTC CTGTGGTGAC GGTGGAGCCT 
CGGCGCCGGA AGTTTCACCG TCCCATCGGC CTAAGAATCC CACTGCCACC CTCATGGAGA 
GAGAGCCCGC GGGACGCCGG AGAGGGAGAC ACCACCAGCC TGCGCCTGCT CTGCAGCGTC 
ATCGGTGGAA CAGCTCCTGC CCAGTGGGAA GATATAACTG GCACCACCAA ACTGGCGTAT 
TCCAATGACT GTGCCAGTTT CACCACCAAC GTCTCTGCCC GATTCTGGCT GGCGGACTGT 
CCTCGGACTG CGGAGGCCGT GTCCTTTGCC AACCTCCTGT ACCGCGAGCT GTCGGCCGTG 
CCGTACATGG CCAAGTTTGT AGTGTTTGCG AAGATGAATG AAGCTCGCGA GGGTCGACTC 
CGCTGCTACT GCATGACGGA TGACAAGATG GACAAAACCC TGGAGCAGCA CGAGAACTTC 
TCGGAAGTGG CTCGCAGCAG AGACATCGAG GTGATGGAGG GAATGCCGCT GTATTTGGAG 
TGTTCAGGAA ACTTGGTTCC TATAAGAAAA GCAGCCCAGC AGCCTCGCTG TTTCAGCTTC 
CAGGCCTTTA AAGACAACAG ACTGCCTGTG TCCGTCAAGG TGAGGGACAG CAGTAAAGAC 
CCCTCTGGGT TTTTGTCTTT CTTGCGCAAG TCAACCAAAT ATGAAGACAG CCAACACGTG 
CTGTGCAACC TCAACGTGAC CATGCCGCCG TGCATTAAGA TTATGGGCAG CGATGAGCGG 
AGGAGGACTC TGACTCCTCT GGCTCTCAGA GAAAGATACA GCGCTCTGAA TGAACCGGCA 
ATGGCATCAC TGAGCGCCAT GGAAAGGACG GAGTTAAAGA TGGCTTTAAT AGCTGAACAG 
CTAGGCCTGA GCTGGGCTGA GTTGGGACGG GAACTGCAGT TTAATGTAGA TGAAATAAAT 
AAGATCCGTG TGGAGAATCC CAACTCCCTT TTAGAGCAGA GCTCAACGCT GCTCAACCTG 
TGGGCCGCCC GAGAGGGGAA GAGAGCCAAA ATGGCGTGTC TCTGTCCTCA TCCTTTATGT 
CCAACTACCC TGTCTTCCCC TTCTCCATTT CTCCACCATC TGTGTGTCTC TCACCACTCT 
AGTGGACAGC CTGTACGTGG CCCTCAAGAG CATCGACCGA GTGGACATTG TGAACATGAT 
GGAGGGCCAG GGAGGACCAC CGGCGGGTCA GCAGGGGGGC TGGGAGCAGG ACGCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331792.1
CDS	225..5084
Translation	MAQAAKHLRKNKDLEALAEQERKEKEEEKSKKRSRSREKKRKAHVVHRWLIDQDNSVSSELPDGQGVWHYDETADAATSFLRAARSGNLEKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLINHGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGLTPLHVSSFMGHLNIVKILMQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGANPNAAGKVGLTPLHVAVHHNNLDVVNLLLSKGGSPHSAARNGYTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPLHLASQEGQPDMVLLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKHGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSNGTSALAIAKRLGYISVIDVLKLVTEETVTMTTTEKHRMSFPETVDEILDVSEDEGIAQLTLGEELLGNEGARYLMKMEDIKDHDEDFLSPKKVLEYEGELENHSPAIPRIPCVSPETVMLKEHEMEQQHTPLPLPKEYDEDSLIPSSPATETSDNVSPVASPIHTGFLVSFMVDARGGSMRGSRHNGLRVIIPPRTCAAPTRITCRLVKPQKLPTPPPLVEGEGLASRIISLGPASMQFLGPVIVEIPHFAALGRGDRELVVLRSENGSVWKEHRNRYGDEVLETILNGMDEDLESQEELGKKRIRRIISTDFPLYFAVVSRIQQENDLIGPEGGFLSSKLVPMVQATFPETAVTKRVRLGLQAQPVPDELVAKLLGNQATFSPVVTVEPRRRKFHRPIGLRIPLPPSWRESPRDAGEGDTTSLRLLCSVIGGTAPAQWEDITGTTKLAYSNDCASFTTNVSARFWLADCPRTAEAVSFANLLYRELSAVPYMAKFVVFAKMNEAREGRLRCYCMTDDKMDKTLEQHENFSEVARSRDIEVMEGMPLYLECSGNLVPIRKAAQQPRCFSFQAFKDNRLPVSVKVRDSSKDPSGFLSFLRKSTKYEDSQHVLCNLNVTMPPCIKIMGSDERRRTLTPLALRERYSALNEPAMASLSAMERTELKMALIAEQLGLSWAELGRELQFNVDEINKIRVENPNSLLEQSSTLLNLWAAREGKRAKMACLCPHPLCPTTLSSPSPFLHHLCVSHHSSGQPVRGPQEHRPSGHCEHDGGPGRTTGGSAGGLGAGRF

Target ORF information:

RefSeq Version	XM_021476117.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X19, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476117.1

ORF Insert Sequence:

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGAATTGC ACAGCTAACA TTAGGCGAGG AGCTGCTGGG AAACGAGGGC 
GCCAGGTATT TGATGAAGAT GGAGGACATA AAGGACCATG ATGAGGATTT CCTTTCCCCC 
AAGAAAGTTC TAGAGTACGA GGGAGAGTTG GAGAATCATT CTCCTGCTAT CCCGAGGATC 
CCGTGTGTGT CTCCTGAGAC TGTCATGCTG AAAGAGCATG AGATGGAGCA GCAGCACACT 
CCACTTCCAT TGCCCAAAGA GTACGACGAG GATTCTCTGA TTCCCAGCAG TCCTGCAACT 
GAGACTTCAG ACAATGTCAG TCCGGTTGCC AGTCCCATCC ACACCGGTTT TCTGGTGAGC 
TTCATGGTGG ATGCTCGCGG CGGCTCGATG CGCGGCAGCA GACACAACGG CCTCCGCGTC 
ATCATTCCTC CTCGAACCTG CGCCGCTCCC ACACGCATCA CCTGCCGCCT GGTGAAGCCC 
CAGAAGCTGC CCACACCGCC GCCATTAGTG GAGGGAGAGG GTCTCGCCAG CCGCATCATC 
TCCCTTGGAC CCGCCAGCAT GCAGTTTTTA GGGCCTGTGA TTGTGGAGAT TCCTCATTTT 
GCGGCTCTCG GTCGGGGAGA TCGGGAACTC GTGGTCTTGA GGAGTGAGAA CGGATCGGTC 
TGGAAGGAAC ATCGTAACCG ATATGGAGAT GAAGTGCTAG AGACCATCCT CAACGGCATG 
GATGAAGATC TGGAGAGTCA GGAGGAGCTC GGGAAGAAAC GCATCAGACG GATCATCTCC 
ACCGACTTCC CGCTCTACTT TGCGGTGGTG TCTCGCATCC AGCAGGAGAA CGATCTGATT 
GGCCCAGAAG GAGGTTTTCT GTCCAGTAAA CTGGTGCCGA TGGTCCAGGC CACCTTTCCA 
GAGACAGCCG TCACCAAACG CGTGCGTCTG GGACTGCAGG CCCAGCCGGT GCCTGATGAG 
CTGGTGGCCA AGCTTTTGGG GAACCAGGCC ACGTTCAGTC CTGTGGTGAC GGTGGAGCCT 
CGGCGCCGGA AGTTTCACCG TCCCATCGGC CTAAGAATCC CACTGCCACC CTCATGGAGA 
GAGAGCCCGC GGGACGCCGG AGAGGGAGAC ACCACCAGCC TGCGCCTGCT CTGCAGCGTC 
ATCGGTGGAA CAGCTCCTGC CCAGTGGGAA GATATAACTG GCACCACCAA ACTGGCGTAT 
TCCAATGACT GTGCCAGTTT CACCACCAAC GTCTCTGCCC GATTCTGGCT GGCGGACTGT 
CCTCGGACTG CGGAGGCCGT GTCCTTTGCC AACCTCCTGT ACCGCGAGCT GTCGGCCGTG 
CCGTACATGG CCAAGTTTGT AGTGTTTGCG AAGATGAATG AAGCTCGCGA GGGTCGACTC 
CGCTGCTACT GCATGACGGA TGACAAGATG GACAAAACCC TGGAGCAGCA CGAGAACTTC 
TCGGAAGTGG CTCGCAGCAG AGACATCGAG GTGATGGAGG GAATGCCGCT GTATTTGGAG 
TGTTCAGGAA ACTTGGTTCC TATAAGAAAA GCAGCCCAGC AGCCTCGCTG TTTCAGCTTC 
CAGGCCTTTA AAGACAACAG ACTGCCTGTG TCCGTCAAGG TGAGGGACAG CAGTAAAGAC 
CCCTCTGGGT TTTTGTCTTT CTTGCGCAAG TCAACCAAAT ATGAAGACAG CCAACACGTG 
CTGTGCAACC TCAACGTGAC CATGCCGCCG TGCATTAAGA TTATGGGCAG CGATGAGCGG 
AGGAGGACTC TGACTCCTCT GGCTCTCAGA GAAAGATACA GCGCTCTGAA TGAACCGGCA 
ATGGCATCAC TGAGCGCCAT GGAAAGGACG GAGTTAAAGA TGGCTTTAAT AGCTGAACAG 
CTAGGCCTGA GCTGGGCTGA GTTGGGACGG GAACTGCAGT TTAATGTAGA TGAAATAAAT 
AAGATCCGTG TGGAGAATCC CAACTCCCTT TTAGAGCAGA GCTCAACGCT GCTCAACCTG 
TGGGCCGCCC GAGAGGGGAA GAGAGCCAAA ATGGCGTGTC TCTGTCCTCA TCCTTTATGT 
CCAACTACCC TGTCTTCCCC TTCTCCATTT CTCCACCATC TGTGTGTCTC TCACCACTCT 
AGTGGACAGC CTGTACGTGG CCCTCAAGAG CATCGACCGA GTGGACATTG TGAACATGAT 
GGAGGGCCAG GGAGGACCAC CGGCGGGTCA GCAGGGGGGC TGGGAGCAGG ACGCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_021476116.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331791.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X18, mRNA.

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CloneID	ODa142608
Clone ID Related Accession (Same CDS sequence)	XM_021476117.1 , XM_021476116.1
Accession Version	XM_021476116.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin 1, erythrocytic a isoform X18
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGAATTGC ACAGCTAACA TTAGGCGAGG AGCTGCTGGG AAACGAGGGC 
GCCAGGTATT TGATGAAGAT GGAGGACATA AAGGACCATG ATGAGGATTT CCTTTCCCCC 
AAGAAAGTTC TAGAGTACGA GGGAGAGTTG GAGAATCATT CTCCTGCTAT CCCGAGGATC 
CCGTGTGTGT CTCCTGAGAC TGTCATGCTG AAAGAGCATG AGATGGAGCA GCAGCACACT 
CCACTTCCAT TGCCCAAAGA GTACGACGAG GATTCTCTGA TTCCCAGCAG TCCTGCAACT 
GAGACTTCAG ACAATGTCAG TCCGGTTGCC AGTCCCATCC ACACCGGTTT TCTGGTGAGC 
TTCATGGTGG ATGCTCGCGG CGGCTCGATG CGCGGCAGCA GACACAACGG CCTCCGCGTC 
ATCATTCCTC CTCGAACCTG CGCCGCTCCC ACACGCATCA CCTGCCGCCT GGTGAAGCCC 
CAGAAGCTGC CCACACCGCC GCCATTAGTG GAGGGAGAGG GTCTCGCCAG CCGCATCATC 
TCCCTTGGAC CCGCCAGCAT GCAGTTTTTA GGGCCTGTGA TTGTGGAGAT TCCTCATTTT 
GCGGCTCTCG GTCGGGGAGA TCGGGAACTC GTGGTCTTGA GGAGTGAGAA CGGATCGGTC 
TGGAAGGAAC ATCGTAACCG ATATGGAGAT GAAGTGCTAG AGACCATCCT CAACGGCATG 
GATGAAGATC TGGAGAGTCA GGAGGAGCTC GGGAAGAAAC GCATCAGACG GATCATCTCC 
ACCGACTTCC CGCTCTACTT TGCGGTGGTG TCTCGCATCC AGCAGGAGAA CGATCTGATT 
GGCCCAGAAG GAGGTTTTCT GTCCAGTAAA CTGGTGCCGA TGGTCCAGGC CACCTTTCCA 
GAGACAGCCG TCACCAAACG CGTGCGTCTG GGACTGCAGG CCCAGCCGGT GCCTGATGAG 
CTGGTGGCCA AGCTTTTGGG GAACCAGGCC ACGTTCAGTC CTGTGGTGAC GGTGGAGCCT 
CGGCGCCGGA AGTTTCACCG TCCCATCGGC CTAAGAATCC CACTGCCACC CTCATGGAGA 
GAGAGCCCGC GGGACGCCGG AGAGGGAGAC ACCACCAGCC TGCGCCTGCT CTGCAGCGTC 
ATCGGTGGAA CAGCTCCTGC CCAGTGGGAA GATATAACTG GCACCACCAA ACTGGCGTAT 
TCCAATGACT GTGCCAGTTT CACCACCAAC GTCTCTGCCC GATTCTGGCT GGCGGACTGT 
CCTCGGACTG CGGAGGCCGT GTCCTTTGCC AACCTCCTGT ACCGCGAGCT GTCGGCCGTG 
CCGTACATGG CCAAGTTTGT AGTGTTTGCG AAGATGAATG AAGCTCGCGA GGGTCGACTC 
CGCTGCTACT GCATGACGGA TGACAAGATG GACAAAACCC TGGAGCAGCA CGAGAACTTC 
TCGGAAGTGG CTCGCAGCAG AGACATCGAG GTGATGGAGG GAATGCCGCT GTATTTGGAG 
TGTTCAGGAA ACTTGGTTCC TATAAGAAAA GCAGCCCAGC AGCCTCGCTG TTTCAGCTTC 
CAGGCCTTTA AAGACAACAG ACTGCCTGTG TCCGTCAAGG TGAGGGACAG CAGTAAAGAC 
CCCTCTGGGT TTTTGTCTTT CTTGCGCAAG TCAACCAAAT ATGAAGACAG CCAACACGTG 
CTGTGCAACC TCAACGTGAC CATGCCGCCG TGCATTAAGA TTATGGGCAG CGATGAGCGG 
AGGAGGACTC TGACTCCTCT GGCTCTCAGA GAAAGATACA GCGCTCTGAA TGAACCGGCA 
ATGGCATCAC TGAGCGCCAT GGAAAGGACG GAGTTAAAGA TGGCTTTAAT AGCTGAACAG 
CTAGGCCTGA GCTGGGCTGA GTTGGGACGG GAACTGCAGT TTAATGTAGA TGAAATAAAT 
AAGATCCGTG TGGAGAATCC CAACTCCCTT TTAGAGCAGA GCTCAACGCT GCTCAACCTG 
TGGGCCGCCC GAGAGGGGAA GAGAGCCAAA ATGGCGTGTC TCTGTCCTCA TCCTTTATGT 
CCAACTACCC TGTCTTCCCC TTCTCCATTT CTCCACCATC TGTGTGTCTC TCACCACTCT 
AGTGGACAGC CTGTACGTGG CCCTCAAGAG CATCGACCGA GTGGACATTG TGAACATGAT 
GGAGGGCCAG GGAGGACCAC CGGCGGGTCA GCAGGGGGGC TGGGAGCAGG ACGCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331791.1
CDS	225..5084
Translation	MAQAAKHLRKNKDLEALAEQERKEKEEEKSKKRSRSREKKRKAHVVHRWLIDQDNSVSSELPDGQGVWHYDETADAATSFLRAARSGNLEKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLINHGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGLTPLHVSSFMGHLNIVKILMQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGANPNAAGKVGLTPLHVAVHHNNLDVVNLLLSKGGSPHSAARNGYTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPLHLASQEGQPDMVLLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKHGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSNGTSALAIAKRLGYISVIDVLKLVTEETVTMTTTEKHRMSFPETVDEILDVSEDEGIAQLTLGEELLGNEGARYLMKMEDIKDHDEDFLSPKKVLEYEGELENHSPAIPRIPCVSPETVMLKEHEMEQQHTPLPLPKEYDEDSLIPSSPATETSDNVSPVASPIHTGFLVSFMVDARGGSMRGSRHNGLRVIIPPRTCAAPTRITCRLVKPQKLPTPPPLVEGEGLASRIISLGPASMQFLGPVIVEIPHFAALGRGDRELVVLRSENGSVWKEHRNRYGDEVLETILNGMDEDLESQEELGKKRIRRIISTDFPLYFAVVSRIQQENDLIGPEGGFLSSKLVPMVQATFPETAVTKRVRLGLQAQPVPDELVAKLLGNQATFSPVVTVEPRRRKFHRPIGLRIPLPPSWRESPRDAGEGDTTSLRLLCSVIGGTAPAQWEDITGTTKLAYSNDCASFTTNVSARFWLADCPRTAEAVSFANLLYRELSAVPYMAKFVVFAKMNEAREGRLRCYCMTDDKMDKTLEQHENFSEVARSRDIEVMEGMPLYLECSGNLVPIRKAAQQPRCFSFQAFKDNRLPVSVKVRDSSKDPSGFLSFLRKSTKYEDSQHVLCNLNVTMPPCIKIMGSDERRRTLTPLALRERYSALNEPAMASLSAMERTELKMALIAEQLGLSWAELGRELQFNVDEINKIRVENPNSLLEQSSTLLNLWAAREGKRAKMACLCPHPLCPTTLSSPSPFLHHLCVSHHSSGQPVRGPQEHRPSGHCEHDGGPGRTTGGSAGGLGAGRF

Target ORF information:

RefSeq Version	XM_021476116.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X18, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476116.1

ORF Insert Sequence:

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGAATTGC ACAGCTAACA TTAGGCGAGG AGCTGCTGGG AAACGAGGGC 
GCCAGGTATT TGATGAAGAT GGAGGACATA AAGGACCATG ATGAGGATTT CCTTTCCCCC 
AAGAAAGTTC TAGAGTACGA GGGAGAGTTG GAGAATCATT CTCCTGCTAT CCCGAGGATC 
CCGTGTGTGT CTCCTGAGAC TGTCATGCTG AAAGAGCATG AGATGGAGCA GCAGCACACT 
CCACTTCCAT TGCCCAAAGA GTACGACGAG GATTCTCTGA TTCCCAGCAG TCCTGCAACT 
GAGACTTCAG ACAATGTCAG TCCGGTTGCC AGTCCCATCC ACACCGGTTT TCTGGTGAGC 
TTCATGGTGG ATGCTCGCGG CGGCTCGATG CGCGGCAGCA GACACAACGG CCTCCGCGTC 
ATCATTCCTC CTCGAACCTG CGCCGCTCCC ACACGCATCA CCTGCCGCCT GGTGAAGCCC 
CAGAAGCTGC CCACACCGCC GCCATTAGTG GAGGGAGAGG GTCTCGCCAG CCGCATCATC 
TCCCTTGGAC CCGCCAGCAT GCAGTTTTTA GGGCCTGTGA TTGTGGAGAT TCCTCATTTT 
GCGGCTCTCG GTCGGGGAGA TCGGGAACTC GTGGTCTTGA GGAGTGAGAA CGGATCGGTC 
TGGAAGGAAC ATCGTAACCG ATATGGAGAT GAAGTGCTAG AGACCATCCT CAACGGCATG 
GATGAAGATC TGGAGAGTCA GGAGGAGCTC GGGAAGAAAC GCATCAGACG GATCATCTCC 
ACCGACTTCC CGCTCTACTT TGCGGTGGTG TCTCGCATCC AGCAGGAGAA CGATCTGATT 
GGCCCAGAAG GAGGTTTTCT GTCCAGTAAA CTGGTGCCGA TGGTCCAGGC CACCTTTCCA 
GAGACAGCCG TCACCAAACG CGTGCGTCTG GGACTGCAGG CCCAGCCGGT GCCTGATGAG 
CTGGTGGCCA AGCTTTTGGG GAACCAGGCC ACGTTCAGTC CTGTGGTGAC GGTGGAGCCT 
CGGCGCCGGA AGTTTCACCG TCCCATCGGC CTAAGAATCC CACTGCCACC CTCATGGAGA 
GAGAGCCCGC GGGACGCCGG AGAGGGAGAC ACCACCAGCC TGCGCCTGCT CTGCAGCGTC 
ATCGGTGGAA CAGCTCCTGC CCAGTGGGAA GATATAACTG GCACCACCAA ACTGGCGTAT 
TCCAATGACT GTGCCAGTTT CACCACCAAC GTCTCTGCCC GATTCTGGCT GGCGGACTGT 
CCTCGGACTG CGGAGGCCGT GTCCTTTGCC AACCTCCTGT ACCGCGAGCT GTCGGCCGTG 
CCGTACATGG CCAAGTTTGT AGTGTTTGCG AAGATGAATG AAGCTCGCGA GGGTCGACTC 
CGCTGCTACT GCATGACGGA TGACAAGATG GACAAAACCC TGGAGCAGCA CGAGAACTTC 
TCGGAAGTGG CTCGCAGCAG AGACATCGAG GTGATGGAGG GAATGCCGCT GTATTTGGAG 
TGTTCAGGAA ACTTGGTTCC TATAAGAAAA GCAGCCCAGC AGCCTCGCTG TTTCAGCTTC 
CAGGCCTTTA AAGACAACAG ACTGCCTGTG TCCGTCAAGG TGAGGGACAG CAGTAAAGAC 
CCCTCTGGGT TTTTGTCTTT CTTGCGCAAG TCAACCAAAT ATGAAGACAG CCAACACGTG 
CTGTGCAACC TCAACGTGAC CATGCCGCCG TGCATTAAGA TTATGGGCAG CGATGAGCGG 
AGGAGGACTC TGACTCCTCT GGCTCTCAGA GAAAGATACA GCGCTCTGAA TGAACCGGCA 
ATGGCATCAC TGAGCGCCAT GGAAAGGACG GAGTTAAAGA TGGCTTTAAT AGCTGAACAG 
CTAGGCCTGA GCTGGGCTGA GTTGGGACGG GAACTGCAGT TTAATGTAGA TGAAATAAAT 
AAGATCCGTG TGGAGAATCC CAACTCCCTT TTAGAGCAGA GCTCAACGCT GCTCAACCTG 
TGGGCCGCCC GAGAGGGGAA GAGAGCCAAA ATGGCGTGTC TCTGTCCTCA TCCTTTATGT 
CCAACTACCC TGTCTTCCCC TTCTCCATTT CTCCACCATC TGTGTGTCTC TCACCACTCT 
AGTGGACAGC CTGTACGTGG CCCTCAAGAG CATCGACCGA GTGGACATTG TGAACATGAT 
GGAGGGCCAG GGAGGACCAC CGGCGGGTCA GCAGGGGGGC TGGGAGCAGG ACGCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_017356074.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211563.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X8, mRNA.

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CloneID	ODa59074
Clone ID Related Accession (Same CDS sequence)	XM_017356074.1
Accession Version	XM_017356074.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5766bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ankyrin 1, erythrocytic a isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAACCA ACACCATCCT TTTTGGAGCT GACGCTGCCA CCAGTTTCCT CCGCGCAGCT 
CGCTCAGGTA ATCTGGAAAA GGCTCTAGAC CACATCAAGA ACGGCATCGA CATCAACACA 
GCCAATCAGA ATGGACTCAA CGGGCTGCAT TTGGCCTCCA AGGAAGGCCA TGTTAAAATG 
GTGCTGGAGC TGCTTCACCA TGGTATTGTA CTTGAGACGA CAACGAAGAA AGGGAACACT 
GCTCTTCATA TAGCGGCGTT GGCTGGCCAG GAGCAGGTGG TCACTGAACT GGTCAACTAT 
GGAGCAAACG TCAACGCTCA GTCTCAGAAG GGCTTCACGC CGCTCTACAT GGCAGCACAA 
GAGAATCATC TGGAGGTTGT GAAGTTTCTG CTGGATAACG GAGCCAATCA GACCATTCCA 
ACTGAGGATG GCTTTACCCC TCTTGCTGTG GCTCTTCAGC AGGGCCATGA GAATGTGGTG 
GCCCTGCTCA TCAACCACGG TACTAAGGGC AAGGTCCGCC TTCCCGCACT GCACATCGCT 
GCCCGAAACG ACGACACCCG CACTGCCGCT GTGCTCTTAC AGAACGACCC CAATCCTGAC 
GTCCTGAGCA AGACAGGCTT CACGCCGCTC CACATCGCAG CACATTATGA AAACCTGAAT 
GTTGCTCAGC TTCTTCTGAA CCGAGGAGCT GATGTGAACT TCACACCTAA GAACGGGATC 
ACACCGCTGC ACATCGCATC CAGGAGAGGG AACGTCATCA TGGTCAGACT TCTGCTGGAC 
CGAGGAGCAA AGATCGATGC CAAGACAAAA GATGAGTTGA CTCCTCTTCA CTGTGCTGCC 
AGAAATGGCC ATGTGAGAAT CATCGAGATC CTTCTGGACC AAGGAGCCCC AATACAGGCC 
AAGACCAAAA ATGGCCTGTC GCCGATCCAC ATGGCAGCCC AGGGCGATCA CTTGGACTGT 
ATCCGACAGC TCTTACAGTA CAACGCAGAG ATCGATGACA TCACTCTGGA CCACCTGACA 
CCTCTGCATG TGGCGGCTCA CTGCGGGCAT CACCGCGTGG CCAAGGTGCT CCTGGATAAA 
GGAGCCAAAC CAAACACCCG CGCTCTGAAT GGATTCACGC CGCTCCACAT TGCCTGTAAG 
AAGAACCACA TGCGTGTAAT GGACCTGCTA TTAAAGCATT CTGCATCCCT GGAAGCTGTA 
ACCGAGTCAG GCTTGACCCC GCTGCATGTG TCCTCCTTCA TGGGTCATCT GAATATAGTC 
AAGATACTGA TGCAAAAAGG AGCTTCGCCG AACGCCTCAA ATGTGAAAGT GGAAACCCCT 
CTGCATATGG CATCTCGAGC TGGACACTGT GAAGTTGCTG AATTCCTATT GCAAAATGCA 
GCTCCAGTTG ATGCCAAAGC CAAGGATGAC CAGACGCCGC TACACTGTGC CTCCCGCATG 
GGCCACAACG AGATGGTGAA GTTGCTTCTG GAGCACAAGG CCAACTCAAA CTCCACCACC 
ACAGCAGGAC ACACACCGCT GCACATCGCG GCCCGCGAGG GACACACACA AACTGCCAGC 
ATCCTCCTGG ACATGAACGC CCAGCTCACC AAAATGACCA AGAAAGGCTT CACTCCTCTT 
CATGTTGCAG CCAAATACGG AAAGGTGGAC GTGGCAGTTC TGCTTCTGGA GAGGGGAGCA 
AATCCAAACG CTGCTGGAAA AGTTGGACTC ACGCCGCTGC ATGTGGCCGT CCATCATAAC 
AATCTAGATG TGGTCAATCT TTTACTCAGC AAAGGAGGAT CACCACACAG CGCCGCTCGG 
AATGGCTATA CTCCGCTCCA CATTGCTTCC AAACAGAACC AGGCGGAGGT GGCGAGCAGT 
TTACTGCAGC ACGGGGCGTC TGCCAATGCT GAGTCTCTGC AAGGGGTCAC ACCTCTACAC 
CTCGCCTCTC AAGAGGGTCA ACCTGACATG GTGTTACTGC TCATCTCCAA GCAGGCCAAT 
GTAAACCTTG GCAATAAGAG CGGTCTGACT CCTCTTCATC TTGTGGCTCA GGAAGGGCAT 
GTTGGGATTG CTGATATACT AGTGAAACAT GGAGCATCTG TTTATGCTGC CACACGGATG 
GGTTACACTC CGCTTCACGT GGCTTGTCAT TATGGGAATA TCAAGATGGT GAAGTTTCTC 
TTACAGCAGC AGGCCAACGT AAACTGCAAG ACCAGGATGA GCTACACGCC GCTACATCAG 
GCTGCTCAGC AGGGTCATAC GGACATCGTG ACGCTTCTGC TGAAACACGG AGCACAACCG 
AATGAAGTTA CATCTAACGG CACCTCTGCT CTTGCCATCG CCAAGCGGCT TGGATACATT 
TCAGTCATCG ATGTGCTCAA ACTGGTCACT GAAGAGACGG TCACTATGAC CACCACTGAA 
AAACATCGCA TGAGTTTCCC TGAAACCGTT GATGAGATCC TGGATGTGTC TGAAGATGAA 
GGAATTGCAC AGCTAACATT AGGCGAGGAG CTGCTGGGAA ACGAGGGCGC CAGGTATTTG 
ATGAAGATGG AGGACATAAA GGACCATGAT GAGGATTTCC TTTCCCCCAA GAAAGTTCTA 
GAGTACGAGG GAGAGTTGGA GAATCATTCT CCTGCTATCC CGAGGATCCC GTGTGTGTCT 
CCTGAGACTG TCATGCTGAA AGAGCATGAG ATGGAGCAGC AGCACACTCC ACTTCCATTG 
CCCAAAGAGT ACGACGAGGA TTCTCTGATT CCCAGCAGTC CTGCAACTGA GACTTCAGAC 
AATGTCAGTC CGGTTGCCAG TCCCATCCAC ACCGGTTTTC TGGTGAGCTT CATGGTGGAT 
GCTCGCGGCG GCTCGATGCG CGGCAGCAGA CACAACGGCC TCCGCGTCAT CATTCCTCCT 
CGAACCTGCG CCGCTCCCAC ACGCATCACC TGCCGCCTGG TGAAGCCCCA GAAGCTGCCC 
ACACCGCCGC CATTAGTGGA GGGAGAGGGT CTCGCCAGCC GCATCATCTC CCTTGGACCC 
GCCAGCATGC AGTTTTTAGG GCCTGTGATT GTGGAGATTC CTCATTTTGC GGCTCTCGGT 
CGGGGAGATC GGGAACTCGT GGTCTTGAGG AGTGAGAACG GATCGGTCTG GAAGGAACAT 
CGTAACCGAT ATGGAGATGA AGTGCTAGAG ACCATCCTCA ACGGCATGGA TGAAGATCTG 
GAGAGTCAGG AGGAGCTCGG GAAGAAACGC ATCAGACGGA TCATCTCCAC CGACTTCCCG 
CTCTACTTTG CGGTGGTGTC TCGCATCCAG CAGGAGAACG ATCTGATTGG CCCAGAAGGA 
GGTTTTCTGT CCAGTAAACT GGTGCCGATG GTCCAGGCCA CCTTTCCAGA GACAGCCGTC 
ACCAAACGCG TGCGTCTGGG ACTGCAGGCC CAGCCGGTGC CTGATGAGCT GGTGGCCAAG 
CTTTTGGGGA ACCAGGCCAC GTTCAGTCCT GTGGTGACGG TGGAGCCTCG GCGCCGGAAG 
TTTCACCGTC CCATCGGCCT AAGAATCCCA CTGCCACCCT CATGGAGAGA GAGCCCGCGG 
GACGCCGGAG AGGGAGACAC CACCAGCCTG CGCCTGCTCT GCAGCGTCAT CGGTGGAACA 
GCTCCTGCCC AGTGGGAAGA TATAACTGGC ACCACCAAAC TGGCGTATTC CAATGACTGT 
GCCAGTTTCA CCACCAACGT CTCTGCCCGA TTCTGGCTGG CGGACTGTCC TCGGACTGCG 
GAGGCCGTGT CCTTTGCCAA CCTCCTGTAC CGCGAGCTGT CGGCCGTGCC GTACATGGCC 
AAGTTTGTAG TGTTTGCGAA GATGAATGAA GCTCGCGAGG GTCGACTCCG CTGCTACTGC 
ATGACGGATG ACAAGATGGA CAAAACCCTG GAGCAGCACG AGAACTTCTC GGAAGTGGCT 
CGCAGCAGAG ACATCGAGGT GATGGAGGGA ATGCCGCTGT ATTTGGAGTG TTCAGGAAAC 
TTGGTTCCTA TAAGAAAAGC AGCCCAGCAG CCTCGCTGTT TCAGCTTCCA GGCCTTTAAA 
GACAACAGAC TGCCTGTGTC CGTCAAGGTG AGGGACAGCA GTAAAGACCC CTCTGGGTTT 
TTGTCTTTCT TGCGCAAGTC AACCAAATAT GAAGACAGCC AACACGTGCT GTGCAACCTC 
AACGTGACCA TGCCGCCGTG CATTAAGATT ATGGGCAGCG ATGAGCGGAG GAGGACTCTG 
ACTCCTCTGG CTCTCAGAGA AAGATACAGC GCTCTGAATG AACCGGCAAT GGCATCACTG 
AGCGCCATGG AAAGGACGGA GTTAAAGATG GCTTTAATAG CTGAACAGCT AGGCCTGAGC 
TGGGCTGAGT TGGGACGGGA ACTGCAGTTT AATGTAGATG AAATAAATAA GATCCGTGTG 
GAGAATCCCA ACTCCCTTTT AGAGCAGAGC TCAACGCTGC TCAACCTGTG GGCCGCCCGA 
GAGGGGAAGA GAGCCAAAAT GGACAGCCTG TACGTGGCCC TCAAGAGCAT CGACCGAGTG 
GACATTGTGA ACATGATGGA GGGCCAGGGA GGACCACCGG CGGGTCAGCA GGGGGGCTGG 
GAGCAGGACG CTTCTAGGTC TAGGCATCAC GACCGAGACC ATCTCTCACC TGGAATCACA 
AATGGTTACG GGCTGGTGGT GCAGGAGGAA CTAGTGTCAC CGGCCTCCAT GCAGTACAGT 
CTGCCCTCGC CACTGGGCAA TGAGCCCTAC TGGCAGGAGG TTTCCAGCAT GGAGTGCGCG 
CCCATCGCCA CCACTGAAGA AGACACGCTC ATGGAGATGT CCGAGGTACA GGTTTGGCCC 
TCAGGAAATA GTCCGTCCAT TGTGGCTGTA GAGGACTCCT CATTGGAGTG CAGCAATGCG 
GACGACTCCG AGGCGCTGCA GAACCGGAGC CTGGGTCACT CCAGGAGCGG GACGCCCGGT 
GGGAACGGAC TCACTCCGGG AGGTTTAAGT GGCTCCATCG AGCTCCTGGA GGATCAGTCC 
ATCGGTGCGG ATTCAGACTA CAGTGCCGCC GGGATGCTGC GTCTGAGCGA AGGGGTCAAC 
ATCAGTGCTC TGGACAGAGG TCAGAGGTCG GAGAGTTCAG AAGGGGCTGT AGGTCACCAA 
GTCACCAACA ACATTAAAGA CGGTTCCAAT GGAGGTTTGG GAGAAGGAGG AGGTGACGGC 
ACACAGTTAG AAGATAGCAT ATCTCTCATT TCAGGGCAAC AGCGTGTGTA TGCTCGAATG 
AGCGAGTCGC CTGGGCTGAG AAGACGGGCA GATCCTGGCG AAGACAGGTT AAGCGCAGGG 
TCGTTCCTGT CTTATCTGCA GGATCAGGGC TCTCCTGGTT GGCATTCGGC TGCAGAAGCG 
ACTCAGAACA GATTGGCGTC TTTGCAATGC TCCCTTCCTA GGCAGGCAAT AGAGTCTATG 
ATGTCATCGG TGCGAGCTAC GGTGGACTCT GACTCCAAGC AGTCTTGTTT GGGCCAGGAG 
GCTCTGATTG AGCCAGTGCG GGACATGGGG CACTCTGAGA TCATCCATGG CCATTATCAG 
GGGACTCAGC CGTTTGAGAA AGGACTCGGC TTCCCGCACA GAACAACCAA CATGCGCACT 
TGGGAGGACA TGCGTCTGAG CGGACAGGGT GATGAGGCTG AGGATCTTCC AGGAGAGCAA 
GTGAGCGAGG AGCAATTCAC AGACGAGCAT GGCAACATCG TCACCAAAAA GCCGGATGTT 
GTGGATGTGA AGAGAGGAGG TGCTCAGATA GTCAAATGTG CGAGTCTGCG GAGAGTAAAG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211563.1
CDS	345..6110
Translation	MPTNTILFGADAATSFLRAARSGNLEKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLINHGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGLTPLHVSSFMGHLNIVKILMQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGANPNAAGKVGLTPLHVAVHHNNLDVVNLLLSKGGSPHSAARNGYTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPLHLASQEGQPDMVLLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKHGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSNGTSALAIAKRLGYISVIDVLKLVTEETVTMTTTEKHRMSFPETVDEILDVSEDEGIAQLTLGEELLGNEGARYLMKMEDIKDHDEDFLSPKKVLEYEGELENHSPAIPRIPCVSPETVMLKEHEMEQQHTPLPLPKEYDEDSLIPSSPATETSDNVSPVASPIHTGFLVSFMVDARGGSMRGSRHNGLRVIIPPRTCAAPTRITCRLVKPQKLPTPPPLVEGEGLASRIISLGPASMQFLGPVIVEIPHFAALGRGDRELVVLRSENGSVWKEHRNRYGDEVLETILNGMDEDLESQEELGKKRIRRIISTDFPLYFAVVSRIQQENDLIGPEGGFLSSKLVPMVQATFPETAVTKRVRLGLQAQPVPDELVAKLLGNQATFSPVVTVEPRRRKFHRPIGLRIPLPPSWRESPRDAGEGDTTSLRLLCSVIGGTAPAQWEDITGTTKLAYSNDCASFTTNVSARFWLADCPRTAEAVSFANLLYRELSAVPYMAKFVVFAKMNEAREGRLRCYCMTDDKMDKTLEQHENFSEVARSRDIEVMEGMPLYLECSGNLVPIRKAAQQPRCFSFQAFKDNRLPVSVKVRDSSKDPSGFLSFLRKSTKYEDSQHVLCNLNVTMPPCIKIMGSDERRRTLTPLALRERYSALNEPAMASLSAMERTELKMALIAEQLGLSWAELGRELQFNVDEINKIRVENPNSLLEQSSTLLNLWAAREGKRAKMDSLYVALKSIDRVDIVNMMEGQGGPPAGQQGGWEQDASRSRHHDRDHLSPGITNGYGLVVQEELVSPASMQYSLPSPLGNEPYWQEVSSMECAPIATTEEDTLMEMSEVQVWPSGNSPSIVAVEDSSLECSNADDSEALQNRSLGHSRSGTPGGNGLTPGGLSGSIELLEDQSIGADSDYSAAGMLRLSEGVNISALDRGQRSESSEGAVGHQVTNNIKDGSNGGLGEGGGDGTQLEDSISLISGQQRVYARMSESPGLRRRADPGEDRLSAGSFLSYLQDQGSPGWHSAAEATQNRLASLQCSLPRQAIESMMSSVRATVDSDSKQSCLGQEALIEPVRDMGHSEIIHGHYQGTQPFEKGLGFPHRTTNMRTWEDMRLSGQGDEAEDLPGEQVSEEQFTDEHGNIVTKKPDVVDVKRGGAQIVKCASLRRVKQ

Target ORF information:

RefSeq Version	XM_017356074.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356074.1

ORF Insert Sequence:

ATGCCAACCA ACACCATCCT TTTTGGAGCT GACGCTGCCA CCAGTTTCCT CCGCGCAGCT 
CGCTCAGGTA ATCTGGAAAA GGCTCTAGAC CACATCAAGA ACGGCATCGA CATCAACACA 
GCCAATCAGA ATGGACTCAA CGGGCTGCAT TTGGCCTCCA AGGAAGGCCA TGTTAAAATG 
GTGCTGGAGC TGCTTCACCA TGGTATTGTA CTTGAGACGA CAACGAAGAA AGGGAACACT 
GCTCTTCATA TAGCGGCGTT GGCTGGCCAG GAGCAGGTGG TCACTGAACT GGTCAACTAT 
GGAGCAAACG TCAACGCTCA GTCTCAGAAG GGCTTCACGC CGCTCTACAT GGCAGCACAA 
GAGAATCATC TGGAGGTTGT GAAGTTTCTG CTGGATAACG GAGCCAATCA GACCATTCCA 
ACTGAGGATG GCTTTACCCC TCTTGCTGTG GCTCTTCAGC AGGGCCATGA GAATGTGGTG 
GCCCTGCTCA TCAACCACGG TACTAAGGGC AAGGTCCGCC TTCCCGCACT GCACATCGCT 
GCCCGAAACG ACGACACCCG CACTGCCGCT GTGCTCTTAC AGAACGACCC CAATCCTGAC 
GTCCTGAGCA AGACAGGCTT CACGCCGCTC CACATCGCAG CACATTATGA AAACCTGAAT 
GTTGCTCAGC TTCTTCTGAA CCGAGGAGCT GATGTGAACT TCACACCTAA GAACGGGATC 
ACACCGCTGC ACATCGCATC CAGGAGAGGG AACGTCATCA TGGTCAGACT TCTGCTGGAC 
CGAGGAGCAA AGATCGATGC CAAGACAAAA GATGAGTTGA CTCCTCTTCA CTGTGCTGCC 
AGAAATGGCC ATGTGAGAAT CATCGAGATC CTTCTGGACC AAGGAGCCCC AATACAGGCC 
AAGACCAAAA ATGGCCTGTC GCCGATCCAC ATGGCAGCCC AGGGCGATCA CTTGGACTGT 
ATCCGACAGC TCTTACAGTA CAACGCAGAG ATCGATGACA TCACTCTGGA CCACCTGACA 
CCTCTGCATG TGGCGGCTCA CTGCGGGCAT CACCGCGTGG CCAAGGTGCT CCTGGATAAA 
GGAGCCAAAC CAAACACCCG CGCTCTGAAT GGATTCACGC CGCTCCACAT TGCCTGTAAG 
AAGAACCACA TGCGTGTAAT GGACCTGCTA TTAAAGCATT CTGCATCCCT GGAAGCTGTA 
ACCGAGTCAG GCTTGACCCC GCTGCATGTG TCCTCCTTCA TGGGTCATCT GAATATAGTC 
AAGATACTGA TGCAAAAAGG AGCTTCGCCG AACGCCTCAA ATGTGAAAGT GGAAACCCCT 
CTGCATATGG CATCTCGAGC TGGACACTGT GAAGTTGCTG AATTCCTATT GCAAAATGCA 
GCTCCAGTTG ATGCCAAAGC CAAGGATGAC CAGACGCCGC TACACTGTGC CTCCCGCATG 
GGCCACAACG AGATGGTGAA GTTGCTTCTG GAGCACAAGG CCAACTCAAA CTCCACCACC 
ACAGCAGGAC ACACACCGCT GCACATCGCG GCCCGCGAGG GACACACACA AACTGCCAGC 
ATCCTCCTGG ACATGAACGC CCAGCTCACC AAAATGACCA AGAAAGGCTT CACTCCTCTT 
CATGTTGCAG CCAAATACGG AAAGGTGGAC GTGGCAGTTC TGCTTCTGGA GAGGGGAGCA 
AATCCAAACG CTGCTGGAAA AGTTGGACTC ACGCCGCTGC ATGTGGCCGT CCATCATAAC 
AATCTAGATG TGGTCAATCT TTTACTCAGC AAAGGAGGAT CACCACACAG CGCCGCTCGG 
AATGGCTATA CTCCGCTCCA CATTGCTTCC AAACAGAACC AGGCGGAGGT GGCGAGCAGT 
TTACTGCAGC ACGGGGCGTC TGCCAATGCT GAGTCTCTGC AAGGGGTCAC ACCTCTACAC 
CTCGCCTCTC AAGAGGGTCA ACCTGACATG GTGTTACTGC TCATCTCCAA GCAGGCCAAT 
GTAAACCTTG GCAATAAGAG CGGTCTGACT CCTCTTCATC TTGTGGCTCA GGAAGGGCAT 
GTTGGGATTG CTGATATACT AGTGAAACAT GGAGCATCTG TTTATGCTGC CACACGGATG 
GGTTACACTC CGCTTCACGT GGCTTGTCAT TATGGGAATA TCAAGATGGT GAAGTTTCTC 
TTACAGCAGC AGGCCAACGT AAACTGCAAG ACCAGGATGA GCTACACGCC GCTACATCAG 
GCTGCTCAGC AGGGTCATAC GGACATCGTG ACGCTTCTGC TGAAACACGG AGCACAACCG 
AATGAAGTTA CATCTAACGG CACCTCTGCT CTTGCCATCG CCAAGCGGCT TGGATACATT 
TCAGTCATCG ATGTGCTCAA ACTGGTCACT GAAGAGACGG TCACTATGAC CACCACTGAA 
AAACATCGCA TGAGTTTCCC TGAAACCGTT GATGAGATCC TGGATGTGTC TGAAGATGAA 
GGAATTGCAC AGCTAACATT AGGCGAGGAG CTGCTGGGAA ACGAGGGCGC CAGGTATTTG 
ATGAAGATGG AGGACATAAA GGACCATGAT GAGGATTTCC TTTCCCCCAA GAAAGTTCTA 
GAGTACGAGG GAGAGTTGGA GAATCATTCT CCTGCTATCC CGAGGATCCC GTGTGTGTCT 
CCTGAGACTG TCATGCTGAA AGAGCATGAG ATGGAGCAGC AGCACACTCC ACTTCCATTG 
CCCAAAGAGT ACGACGAGGA TTCTCTGATT CCCAGCAGTC CTGCAACTGA GACTTCAGAC 
AATGTCAGTC CGGTTGCCAG TCCCATCCAC ACCGGTTTTC TGGTGAGCTT CATGGTGGAT 
GCTCGCGGCG GCTCGATGCG CGGCAGCAGA CACAACGGCC TCCGCGTCAT CATTCCTCCT 
CGAACCTGCG CCGCTCCCAC ACGCATCACC TGCCGCCTGG TGAAGCCCCA GAAGCTGCCC 
ACACCGCCGC CATTAGTGGA GGGAGAGGGT CTCGCCAGCC GCATCATCTC CCTTGGACCC 
GCCAGCATGC AGTTTTTAGG GCCTGTGATT GTGGAGATTC CTCATTTTGC GGCTCTCGGT 
CGGGGAGATC GGGAACTCGT GGTCTTGAGG AGTGAGAACG GATCGGTCTG GAAGGAACAT 
CGTAACCGAT ATGGAGATGA AGTGCTAGAG ACCATCCTCA ACGGCATGGA TGAAGATCTG 
GAGAGTCAGG AGGAGCTCGG GAAGAAACGC ATCAGACGGA TCATCTCCAC CGACTTCCCG 
CTCTACTTTG CGGTGGTGTC TCGCATCCAG CAGGAGAACG ATCTGATTGG CCCAGAAGGA 
GGTTTTCTGT CCAGTAAACT GGTGCCGATG GTCCAGGCCA CCTTTCCAGA GACAGCCGTC 
ACCAAACGCG TGCGTCTGGG ACTGCAGGCC CAGCCGGTGC CTGATGAGCT GGTGGCCAAG 
CTTTTGGGGA ACCAGGCCAC GTTCAGTCCT GTGGTGACGG TGGAGCCTCG GCGCCGGAAG 
TTTCACCGTC CCATCGGCCT AAGAATCCCA CTGCCACCCT CATGGAGAGA GAGCCCGCGG 
GACGCCGGAG AGGGAGACAC CACCAGCCTG CGCCTGCTCT GCAGCGTCAT CGGTGGAACA 
GCTCCTGCCC AGTGGGAAGA TATAACTGGC ACCACCAAAC TGGCGTATTC CAATGACTGT 
GCCAGTTTCA CCACCAACGT CTCTGCCCGA TTCTGGCTGG CGGACTGTCC TCGGACTGCG 
GAGGCCGTGT CCTTTGCCAA CCTCCTGTAC CGCGAGCTGT CGGCCGTGCC GTACATGGCC 
AAGTTTGTAG TGTTTGCGAA GATGAATGAA GCTCGCGAGG GTCGACTCCG CTGCTACTGC 
ATGACGGATG ACAAGATGGA CAAAACCCTG GAGCAGCACG AGAACTTCTC GGAAGTGGCT 
CGCAGCAGAG ACATCGAGGT GATGGAGGGA ATGCCGCTGT ATTTGGAGTG TTCAGGAAAC 
TTGGTTCCTA TAAGAAAAGC AGCCCAGCAG CCTCGCTGTT TCAGCTTCCA GGCCTTTAAA 
GACAACAGAC TGCCTGTGTC CGTCAAGGTG AGGGACAGCA GTAAAGACCC CTCTGGGTTT 
TTGTCTTTCT TGCGCAAGTC AACCAAATAT GAAGACAGCC AACACGTGCT GTGCAACCTC 
AACGTGACCA TGCCGCCGTG CATTAAGATT ATGGGCAGCG ATGAGCGGAG GAGGACTCTG 
ACTCCTCTGG CTCTCAGAGA AAGATACAGC GCTCTGAATG AACCGGCAAT GGCATCACTG 
AGCGCCATGG AAAGGACGGA GTTAAAGATG GCTTTAATAG CTGAACAGCT AGGCCTGAGC 
TGGGCTGAGT TGGGACGGGA ACTGCAGTTT AATGTAGATG AAATAAATAA GATCCGTGTG 
GAGAATCCCA ACTCCCTTTT AGAGCAGAGC TCAACGCTGC TCAACCTGTG GGCCGCCCGA 
GAGGGGAAGA GAGCCAAAAT GGACAGCCTG TACGTGGCCC TCAAGAGCAT CGACCGAGTG 
GACATTGTGA ACATGATGGA GGGCCAGGGA GGACCACCGG CGGGTCAGCA GGGGGGCTGG 
GAGCAGGACG CTTCTAGGTC TAGGCATCAC GACCGAGACC ATCTCTCACC TGGAATCACA 
AATGGTTACG GGCTGGTGGT GCAGGAGGAA CTAGTGTCAC CGGCCTCCAT GCAGTACAGT 
CTGCCCTCGC CACTGGGCAA TGAGCCCTAC TGGCAGGAGG TTTCCAGCAT GGAGTGCGCG 
CCCATCGCCA CCACTGAAGA AGACACGCTC ATGGAGATGT CCGAGGTACA GGTTTGGCCC 
TCAGGAAATA GTCCGTCCAT TGTGGCTGTA GAGGACTCCT CATTGGAGTG CAGCAATGCG 
GACGACTCCG AGGCGCTGCA GAACCGGAGC CTGGGTCACT CCAGGAGCGG GACGCCCGGT 
GGGAACGGAC TCACTCCGGG AGGTTTAAGT GGCTCCATCG AGCTCCTGGA GGATCAGTCC 
ATCGGTGCGG ATTCAGACTA CAGTGCCGCC GGGATGCTGC GTCTGAGCGA AGGGGTCAAC 
ATCAGTGCTC TGGACAGAGG TCAGAGGTCG GAGAGTTCAG AAGGGGCTGT AGGTCACCAA 
GTCACCAACA ACATTAAAGA CGGTTCCAAT GGAGGTTTGG GAGAAGGAGG AGGTGACGGC 
ACACAGTTAG AAGATAGCAT ATCTCTCATT TCAGGGCAAC AGCGTGTGTA TGCTCGAATG 
AGCGAGTCGC CTGGGCTGAG AAGACGGGCA GATCCTGGCG AAGACAGGTT AAGCGCAGGG 
TCGTTCCTGT CTTATCTGCA GGATCAGGGC TCTCCTGGTT GGCATTCGGC TGCAGAAGCG 
ACTCAGAACA GATTGGCGTC TTTGCAATGC TCCCTTCCTA GGCAGGCAAT AGAGTCTATG 
ATGTCATCGG TGCGAGCTAC GGTGGACTCT GACTCCAAGC AGTCTTGTTT GGGCCAGGAG 
GCTCTGATTG AGCCAGTGCG GGACATGGGG CACTCTGAGA TCATCCATGG CCATTATCAG 
GGGACTCAGC CGTTTGAGAA AGGACTCGGC TTCCCGCACA GAACAACCAA CATGCGCACT 
TGGGAGGACA TGCGTCTGAG CGGACAGGGT GATGAGGCTG AGGATCTTCC AGGAGAGCAA 
GTGAGCGAGG AGCAATTCAC AGACGAGCAT GGCAACATCG TCACCAAAAA GCCGGATGTT 
GTGGATGTGA AGAGAGGAGG TGCTCAGATA GTCAAATGTG CGAGTCTGCG GAGAGTAAAG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_017356075.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017211564.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X9, mRNA.

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CloneID	ODa59075
Clone ID Related Accession (Same CDS sequence)	XM_017356075.1
Accession Version	XM_017356075.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5733bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ankyrin 1, erythrocytic a isoform X9
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAACCA ACACCATCCT TTTTGGAGCT GACGCTGCCA CCAGTTTCCT CCGCGCAGCT 
CGCTCAGGTA ATCTGGAAAA GGCTCTAGAC CACATCAAGA ACGGCATCGA CATCAACACA 
GCCAATCAGA ATGGACTCAA CGGGCTGCAT TTGGCCTCCA AGGAAGGCCA TGTTAAAATG 
GTGCTGGAGC TGCTTCACCA TGGTATTGTA CTTGAGACGA CAACGAAGAA AGGGAACACT 
GCTCTTCATA TAGCGGCGTT GGCTGGCCAG GAGCAGGTGG TCACTGAACT GGTCAACTAT 
GGAGCAAACG TCAACGCTCA GTCTCAGAAG GGCTTCACGC CGCTCTACAT GGCAGCACAA 
GAGAATCATC TGGAGGTTGT GAAGTTTCTG CTGGATAACG GAGCCAATCA GACCATTCCA 
ACTGAGGATG GCTTTACCCC TCTTGCTGTG GCTCTTCAGC AGGGCCATGA GAATGTGGTG 
GCCCTGCTCA TCAACCACGG TACTAAGGGC AAGGTCCGCC TTCCCGCACT GCACATCGCT 
GCCCGAAACG ACGACACCCG CACTGCCGCT GTGCTCTTAC AGAACGACCC CAATCCTGAC 
GTCCTGAGCA AGACAGGCTT CACGCCGCTC CACATCGCAG CACATTATGA AAACCTGAAT 
GTTGCTCAGC TTCTTCTGAA CCGAGGAGCT GATGTGAACT TCACACCTAA GAACGGGATC 
ACACCGCTGC ACATCGCATC CAGGAGAGGG AACGTCATCA TGGTCAGACT TCTGCTGGAC 
CGAGGAGCAA AGATCGATGC CAAGACAAAA GATGAGTTGA CTCCTCTTCA CTGTGCTGCC 
AGAAATGGCC ATGTGAGAAT CATCGAGATC CTTCTGGACC AAGGAGCCCC AATACAGGCC 
AAGACCAAAA ATGGCCTGTC GCCGATCCAC ATGGCAGCCC AGGGCGATCA CTTGGACTGT 
ATCCGACAGC TCTTACAGTA CAACGCAGAG ATCGATGACA TCACTCTGGA CCACCTGACA 
CCTCTGCATG TGGCGGCTCA CTGCGGGCAT CACCGCGTGG CCAAGGTGCT CCTGGATAAA 
GGAGCCAAAC CAAACACCCG CGCTCTGAAT GGATTCACGC CGCTCCACAT TGCCTGTAAG 
AAGAACCACA TGCGTGTAAT GGACCTGCTA TTAAAGCATT CTGCATCCCT GGAAGCTGTA 
ACCGAGTCAG GCTTGACCCC GCTGCATGTG TCCTCCTTCA TGGGTCATCT GAATATAGTC 
AAGATACTGA TGCAAAAAGG AGCTTCGCCG AACGCCTCAA ATGTGAAAGT GGAAACCCCT 
CTGCATATGG CATCTCGAGC TGGACACTGT GAAGTTGCTG AATTCCTATT GCAAAATGCA 
GCTCCAGTTG ATGCCAAAGC CAAGGATGAC CAGACGCCGC TACACTGTGC CTCCCGCATG 
GGCCACAACG AGATGGTGAA GTTGCTTCTG GAGCACAAGG CCAACTCAAA CTCCACCACC 
ACAGCAGGAC ACACACCGCT GCACATCGCG GCCCGCGAGG GACACACACA AACTGCCAGC 
ATCCTCCTGG ACATGAACGC CCAGCTCACC AAAATGACCA AGAAAGGCTT CACTCCTCTT 
CATGTTGCAG CCAAATACGG AAAGGTGGAC GTGGCAGTTC TGCTTCTGGA GAGGGGAGCA 
AATCCAAACG CTGCTGGAAA AGTTGGACTC ACGCCGCTGC ATGTGGCCGT CCATCATAAC 
AATCTAGATG TGGTCAATCT TTTACTCAGC AAAGGAGGAT CACCACACAG CGCCGCTCGG 
AATGGCTATA CTCCGCTCCA CATTGCTTCC AAACAGAACC AGGCGGAGGT GGCGAGCAGT 
TTACTGCAGC ACGGGGCGTC TGCCAATGCT GAGTCTCTGC AAGGGGTCAC ACCTCTACAC 
CTCGCCTCTC AAGAGGGTCA ACCTGACATG GTGTTACTGC TCATCTCCAA GCAGGCCAAT 
GTAAACCTTG GCAATAAGAG CGGTCTGACT CCTCTTCATC TTGTGGCTCA GGAAGGGCAT 
GTTGGGATTG CTGATATACT AGTGAAACAT GGAGCATCTG TTTATGCTGC CACACGGATG 
GGTTACACTC CGCTTCACGT GGCTTGTCAT TATGGGAATA TCAAGATGGT GAAGTTTCTC 
TTACAGCAGC AGGCCAACGT AAACTGCAAG ACCAGGATGA GCTACACGCC GCTACATCAG 
GCTGCTCAGC AGGGTCATAC GGACATCGTG ACGCTTCTGC TGAAACACGG AGCACAACCG 
AATGAAGTTA CATCTAACGG CACCTCTGCT CTTGCCATCG CCAAGCGGCT TGGATACATT 
TCAGTCATCG ATGTGCTCAA ACTGGTCACT GAAGAGACGG TCACTATGAC CACCACTGAA 
AAACATCGCA TGAGTTTCCC TGAAACCGTT GATGAGATCC TGGATGTGTC TGAAGATGAA 
GGAATTGCAC AGCTAACATT AGGCGAGGAG CTGCTGGGAA ACGAGGGCGC CAGGTATTTG 
ATGAAGATGG AGGACATAAA GGACCATGAT GAGGATTTCC TTTCCCCCAA GAAAGTTCTA 
GAGTACGAGG GAGAGTTGGA GAATCATTCT CCTGCTATCC CGAGGATCCC GTGTGTGTCT 
CCTGAGACTG TCATGCTGAA AGAGCATGAG ATGGAGCAGC AGCACACTCC ACTTCCATTG 
CCCAAAGAGT ACGACGAGGA TTCTCTGATT CCCAGCAGTC CTGCAACTGA GACTTCAGAC 
AATGTCAGTC CGGTTGCCAG TCCCATCCAC ACCGGTTTTC TGGTGAGCTT CATGGTGGAT 
GCTCGCGGCG GCTCGATGCG CGGCAGCAGA CACAACGGCC TCCGCGTCAT CATTCCTCCT 
CGAACCTGCG CCGCTCCCAC ACGCATCACC TGCCGCCTGG TGAAGCCCCA GAAGCTGCCC 
ACACCGCCGC CATTAGTGGA GGGAGAGGGT CTCGCCAGCC GCATCATCTC CCTTGGACCC 
GCCAGCATGC AGTTTTTAGG GCCTGTGATT GTGGAGATTC CTCATTTTGC GGCTCTCGGT 
CGGGGAGATC GGGAACTCGT GGTCTTGAGG AGTGAGAACG GATCGGTCTG GAAGGAACAT 
CGTAACCGAT ATGGAGATGA AGTGCTAGAG ACCATCCTCA ACGGCATGGA TGAAGATCTG 
GAGAGTCAGG AGGAGCTCGG GAAGAAACGC ATCAGACGGA TCATCTCCAC CGACTTCCCG 
CTCTACTTTG CGGTGGTGTC TCGCATCCAG CAGGAGAACG ATCTGATTGG CCCAGAAGGA 
GGTTTTCTGT CCAGTAAACT GGTGCCGATG GTCCAGGCCA CCTTTCCAGA GACAGCCGTC 
ACCAAACGCG TGCGTCTGGG ACTGCAGGCC CAGCCGGTGC CTGATGAGCT GGTGGCCAAG 
CTTTTGGGGA ACCAGGCCAC GTTCAGTCCT GTGGTGACGG TGGAGCCTCG GCGCCGGAAG 
TTTCACCGTC CCATCGGCCT AAGAATCCCA CTGCCACCCT CATGGAGAGA GAGCCCGCGG 
GACGCCGGAG AGGGAGACAC CACCAGCCTG CGCCTGCTCT GCAGCGTCAT CGGTGGAACA 
GCTCCTGCCC AGTGGGAAGA TATAACTGGC ACCACCAAAC TGGCGTATTC CAATGACTGT 
GCCAGTTTCA CCACCAACGT CTCTGCCCGA TTCTGGCTGG CGGACTGTCC TCGGACTGCG 
GAGGCCGTGT CCTTTGCCAA CCTCCTGTAC CGCGAGCTGT CGGCCGTGCC GTACATGGCC 
AAGTTTGTAG TGTTTGCGAA GATGAATGAA GCTCGCGAGG GTCGACTCCG CTGCTACTGC 
ATGACGGATG ACAAGATGGA CAAAACCCTG GAGCAGCACG AGAACTTCTC GGAAGTGGCT 
CGCAGCAGAG ACATCGAGGT GATGGAGGGA ATGCCGCTGT ATTTGGAGTG TTCAGGAAAC 
TTGGTTCCTA TAAGAAAAGC AGCCCAGCAG CCTCGCTGTT TCAGCTTCCA GGCCTTTAAA 
GACAACAGAC TGCCTGTGTC CGTCAAGGTG AGGGACAGCA GTAAAGACCC CTCTGGGTTT 
TTGTCTTTCT TGCGCAAGTC AACCAAATAT GAAGACAGCC AACACGTGCT GTGCAACCTC 
AACGTGACCA TGCCGCCGTG CATTAAGATT ATGGGCAGCG ATGAGCGGAG GAGGACTCTG 
ACTCCTCTGG CTCTCAGAGA AAGATACAGC GCTCTGAATG AACCGGCAAT GGCATCACTG 
AGCGCCATGG AAAGGACGGA GTTAAAGATG GCTTTAATAG CTGAACAGCT AGGCCTGAGC 
TGGGCTGAGT TGGGACGGGA ACTGCAGTTT AATGTAGATG AAATAAATAA GATCCGTGTG 
GAGAATCCCA ACTCCCTTTT AGAGCAGAGC TCAACGCTGC TCAACCTGTG GGCCGCCCGA 
GAGGGGAAGA GAGCCAAAAT GGACAGCCTG TACGTGGCCC TCAAGAGCAT CGACCGAGTG 
GACATTGTGA ACATGATGGA GGGCCAGGGA GGACCACCGG CGGGTCAGCA GGGGGGCTGG 
GAGCAGGACG CTTCTAGGTC TAGGCATCAC GACCGAGACC ATCTCTCACC TGGAATCACA 
AATGGTTACG GGCTGGTGGT GCAGGAGGAA CTAGTGTCAC CGGCCTCCAT GCAGTACAGT 
CTGCCCTCGC CACTGGGCAA TGAGCCCTAC TGGCAGGAGG TTTCCAGCAT GGAGTGCGCG 
CCCATCGCCA CCACTGAAGA AGACACGCTC ATGGAGATGT CCGAGGTACA GGTTTGGCCC 
TCAGGAAATA GTCCGTCCAT TGTGGCTGTA GAGGACTCCT CATTGGAGTG CAGCAATGCG 
GACGACTCCG AGGCGCTGCA GAACCGGAGC CTGGGTCACT CCAGGAGCGG GACGCCCGGT 
GGGAACGGAC TCACTCCGGG AGGTTTAAGT GGCTCCATCG AGCTCCTGGA GGATCAGTCC 
ATCGGTGCGG ATTCAGACTA CAGTGCCGCC GGGATGCTGC GTCTGAGCGA AGGGGTCAAC 
ATCAGTGCTC TGGACAGAGG TCAGAGGTCG GAGAGTTCAG AAGGGGCTGT AGGTCACCAA 
GTCACCAACA ACATTAAAGA CGGTTCCAAT GGAGGTTTGG GAGAAGGAGG AGGTGACGGC 
ACACAGTTAG AAGATAGCAT ATCTCTCATT TCAGGGCAAC AGCGTGTGTA TGCTCGAATG 
AGCGAGTCGC CTGGGCTGAG AAGACGGGCA GATCCTGGCG AAGACAGGTT AAGCGCAGGG 
TCGTTCCTGT CTTATCTGCA GGATCAGGGC TCTCCTGGTT GGCATTCGGC TGCAGAAGCG 
ACTCAGAACA GATTGGCGTC TTTGCAATGC TCCCTTCCTA GGCAGGCAAT AGAGTCTATG 
ATGTCATCGG TGCGAGCTAC GGTGGACTCT GACTCCAAGC AGTCTTGTTT GGGCCAGGAG 
GCTCTGATTG AGCCAGTGCG GGACATGGGG CACTCTGAGA TCATCCATGG CCATTATCAG 
GGGACTCAGC CGTTTGAGAA AGGACTCGGC TTCCCGCACA GAACAACCAA CATGCGCACT 
TGGGAGGACA TGCGTCTGAG CGGACAGGGT GATGAGGCTG AGGATCTTCC AGGAGAGCAA 
GTGAGCGAGG AGCAATTCAC AGACGAGCAT GGCAACATCG TCACCAAAAA GGACTCGACT 
CCTTCACCCA AACCATCCTT CACTGACTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017211564.1
CDS	344..6076
Translation	MPTNTILFGADAATSFLRAARSGNLEKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLINHGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGLTPLHVSSFMGHLNIVKILMQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGANPNAAGKVGLTPLHVAVHHNNLDVVNLLLSKGGSPHSAARNGYTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPLHLASQEGQPDMVLLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKHGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSNGTSALAIAKRLGYISVIDVLKLVTEETVTMTTTEKHRMSFPETVDEILDVSEDEGIAQLTLGEELLGNEGARYLMKMEDIKDHDEDFLSPKKVLEYEGELENHSPAIPRIPCVSPETVMLKEHEMEQQHTPLPLPKEYDEDSLIPSSPATETSDNVSPVASPIHTGFLVSFMVDARGGSMRGSRHNGLRVIIPPRTCAAPTRITCRLVKPQKLPTPPPLVEGEGLASRIISLGPASMQFLGPVIVEIPHFAALGRGDRELVVLRSENGSVWKEHRNRYGDEVLETILNGMDEDLESQEELGKKRIRRIISTDFPLYFAVVSRIQQENDLIGPEGGFLSSKLVPMVQATFPETAVTKRVRLGLQAQPVPDELVAKLLGNQATFSPVVTVEPRRRKFHRPIGLRIPLPPSWRESPRDAGEGDTTSLRLLCSVIGGTAPAQWEDITGTTKLAYSNDCASFTTNVSARFWLADCPRTAEAVSFANLLYRELSAVPYMAKFVVFAKMNEAREGRLRCYCMTDDKMDKTLEQHENFSEVARSRDIEVMEGMPLYLECSGNLVPIRKAAQQPRCFSFQAFKDNRLPVSVKVRDSSKDPSGFLSFLRKSTKYEDSQHVLCNLNVTMPPCIKIMGSDERRRTLTPLALRERYSALNEPAMASLSAMERTELKMALIAEQLGLSWAELGRELQFNVDEINKIRVENPNSLLEQSSTLLNLWAAREGKRAKMDSLYVALKSIDRVDIVNMMEGQGGPPAGQQGGWEQDASRSRHHDRDHLSPGITNGYGLVVQEELVSPASMQYSLPSPLGNEPYWQEVSSMECAPIATTEEDTLMEMSEVQVWPSGNSPSIVAVEDSSLECSNADDSEALQNRSLGHSRSGTPGGNGLTPGGLSGSIELLEDQSIGADSDYSAAGMLRLSEGVNISALDRGQRSESSEGAVGHQVTNNIKDGSNGGLGEGGGDGTQLEDSISLISGQQRVYARMSESPGLRRRADPGEDRLSAGSFLSYLQDQGSPGWHSAAEATQNRLASLQCSLPRQAIESMMSSVRATVDSDSKQSCLGQEALIEPVRDMGHSEIIHGHYQGTQPFEKGLGFPHRTTNMRTWEDMRLSGQGDEAEDLPGEQVSEEQFTDEHGNIVTKKDSTPSPKPSFTDS

Target ORF information:

RefSeq Version	XM_017356075.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356075.1

ORF Insert Sequence:

ATGCCAACCA ACACCATCCT TTTTGGAGCT GACGCTGCCA CCAGTTTCCT CCGCGCAGCT 
CGCTCAGGTA ATCTGGAAAA GGCTCTAGAC CACATCAAGA ACGGCATCGA CATCAACACA 
GCCAATCAGA ATGGACTCAA CGGGCTGCAT TTGGCCTCCA AGGAAGGCCA TGTTAAAATG 
GTGCTGGAGC TGCTTCACCA TGGTATTGTA CTTGAGACGA CAACGAAGAA AGGGAACACT 
GCTCTTCATA TAGCGGCGTT GGCTGGCCAG GAGCAGGTGG TCACTGAACT GGTCAACTAT 
GGAGCAAACG TCAACGCTCA GTCTCAGAAG GGCTTCACGC CGCTCTACAT GGCAGCACAA 
GAGAATCATC TGGAGGTTGT GAAGTTTCTG CTGGATAACG GAGCCAATCA GACCATTCCA 
ACTGAGGATG GCTTTACCCC TCTTGCTGTG GCTCTTCAGC AGGGCCATGA GAATGTGGTG 
GCCCTGCTCA TCAACCACGG TACTAAGGGC AAGGTCCGCC TTCCCGCACT GCACATCGCT 
GCCCGAAACG ACGACACCCG CACTGCCGCT GTGCTCTTAC AGAACGACCC CAATCCTGAC 
GTCCTGAGCA AGACAGGCTT CACGCCGCTC CACATCGCAG CACATTATGA AAACCTGAAT 
GTTGCTCAGC TTCTTCTGAA CCGAGGAGCT GATGTGAACT TCACACCTAA GAACGGGATC 
ACACCGCTGC ACATCGCATC CAGGAGAGGG AACGTCATCA TGGTCAGACT TCTGCTGGAC 
CGAGGAGCAA AGATCGATGC CAAGACAAAA GATGAGTTGA CTCCTCTTCA CTGTGCTGCC 
AGAAATGGCC ATGTGAGAAT CATCGAGATC CTTCTGGACC AAGGAGCCCC AATACAGGCC 
AAGACCAAAA ATGGCCTGTC GCCGATCCAC ATGGCAGCCC AGGGCGATCA CTTGGACTGT 
ATCCGACAGC TCTTACAGTA CAACGCAGAG ATCGATGACA TCACTCTGGA CCACCTGACA 
CCTCTGCATG TGGCGGCTCA CTGCGGGCAT CACCGCGTGG CCAAGGTGCT CCTGGATAAA 
GGAGCCAAAC CAAACACCCG CGCTCTGAAT GGATTCACGC CGCTCCACAT TGCCTGTAAG 
AAGAACCACA TGCGTGTAAT GGACCTGCTA TTAAAGCATT CTGCATCCCT GGAAGCTGTA 
ACCGAGTCAG GCTTGACCCC GCTGCATGTG TCCTCCTTCA TGGGTCATCT GAATATAGTC 
AAGATACTGA TGCAAAAAGG AGCTTCGCCG AACGCCTCAA ATGTGAAAGT GGAAACCCCT 
CTGCATATGG CATCTCGAGC TGGACACTGT GAAGTTGCTG AATTCCTATT GCAAAATGCA 
GCTCCAGTTG ATGCCAAAGC CAAGGATGAC CAGACGCCGC TACACTGTGC CTCCCGCATG 
GGCCACAACG AGATGGTGAA GTTGCTTCTG GAGCACAAGG CCAACTCAAA CTCCACCACC 
ACAGCAGGAC ACACACCGCT GCACATCGCG GCCCGCGAGG GACACACACA AACTGCCAGC 
ATCCTCCTGG ACATGAACGC CCAGCTCACC AAAATGACCA AGAAAGGCTT CACTCCTCTT 
CATGTTGCAG CCAAATACGG AAAGGTGGAC GTGGCAGTTC TGCTTCTGGA GAGGGGAGCA 
AATCCAAACG CTGCTGGAAA AGTTGGACTC ACGCCGCTGC ATGTGGCCGT CCATCATAAC 
AATCTAGATG TGGTCAATCT TTTACTCAGC AAAGGAGGAT CACCACACAG CGCCGCTCGG 
AATGGCTATA CTCCGCTCCA CATTGCTTCC AAACAGAACC AGGCGGAGGT GGCGAGCAGT 
TTACTGCAGC ACGGGGCGTC TGCCAATGCT GAGTCTCTGC AAGGGGTCAC ACCTCTACAC 
CTCGCCTCTC AAGAGGGTCA ACCTGACATG GTGTTACTGC TCATCTCCAA GCAGGCCAAT 
GTAAACCTTG GCAATAAGAG CGGTCTGACT CCTCTTCATC TTGTGGCTCA GGAAGGGCAT 
GTTGGGATTG CTGATATACT AGTGAAACAT GGAGCATCTG TTTATGCTGC CACACGGATG 
GGTTACACTC CGCTTCACGT GGCTTGTCAT TATGGGAATA TCAAGATGGT GAAGTTTCTC 
TTACAGCAGC AGGCCAACGT AAACTGCAAG ACCAGGATGA GCTACACGCC GCTACATCAG 
GCTGCTCAGC AGGGTCATAC GGACATCGTG ACGCTTCTGC TGAAACACGG AGCACAACCG 
AATGAAGTTA CATCTAACGG CACCTCTGCT CTTGCCATCG CCAAGCGGCT TGGATACATT 
TCAGTCATCG ATGTGCTCAA ACTGGTCACT GAAGAGACGG TCACTATGAC CACCACTGAA 
AAACATCGCA TGAGTTTCCC TGAAACCGTT GATGAGATCC TGGATGTGTC TGAAGATGAA 
GGAATTGCAC AGCTAACATT AGGCGAGGAG CTGCTGGGAA ACGAGGGCGC CAGGTATTTG 
ATGAAGATGG AGGACATAAA GGACCATGAT GAGGATTTCC TTTCCCCCAA GAAAGTTCTA 
GAGTACGAGG GAGAGTTGGA GAATCATTCT CCTGCTATCC CGAGGATCCC GTGTGTGTCT 
CCTGAGACTG TCATGCTGAA AGAGCATGAG ATGGAGCAGC AGCACACTCC ACTTCCATTG 
CCCAAAGAGT ACGACGAGGA TTCTCTGATT CCCAGCAGTC CTGCAACTGA GACTTCAGAC 
AATGTCAGTC CGGTTGCCAG TCCCATCCAC ACCGGTTTTC TGGTGAGCTT CATGGTGGAT 
GCTCGCGGCG GCTCGATGCG CGGCAGCAGA CACAACGGCC TCCGCGTCAT CATTCCTCCT 
CGAACCTGCG CCGCTCCCAC ACGCATCACC TGCCGCCTGG TGAAGCCCCA GAAGCTGCCC 
ACACCGCCGC CATTAGTGGA GGGAGAGGGT CTCGCCAGCC GCATCATCTC CCTTGGACCC 
GCCAGCATGC AGTTTTTAGG GCCTGTGATT GTGGAGATTC CTCATTTTGC GGCTCTCGGT 
CGGGGAGATC GGGAACTCGT GGTCTTGAGG AGTGAGAACG GATCGGTCTG GAAGGAACAT 
CGTAACCGAT ATGGAGATGA AGTGCTAGAG ACCATCCTCA ACGGCATGGA TGAAGATCTG 
GAGAGTCAGG AGGAGCTCGG GAAGAAACGC ATCAGACGGA TCATCTCCAC CGACTTCCCG 
CTCTACTTTG CGGTGGTGTC TCGCATCCAG CAGGAGAACG ATCTGATTGG CCCAGAAGGA 
GGTTTTCTGT CCAGTAAACT GGTGCCGATG GTCCAGGCCA CCTTTCCAGA GACAGCCGTC 
ACCAAACGCG TGCGTCTGGG ACTGCAGGCC CAGCCGGTGC CTGATGAGCT GGTGGCCAAG 
CTTTTGGGGA ACCAGGCCAC GTTCAGTCCT GTGGTGACGG TGGAGCCTCG GCGCCGGAAG 
TTTCACCGTC CCATCGGCCT AAGAATCCCA CTGCCACCCT CATGGAGAGA GAGCCCGCGG 
GACGCCGGAG AGGGAGACAC CACCAGCCTG CGCCTGCTCT GCAGCGTCAT CGGTGGAACA 
GCTCCTGCCC AGTGGGAAGA TATAACTGGC ACCACCAAAC TGGCGTATTC CAATGACTGT 
GCCAGTTTCA CCACCAACGT CTCTGCCCGA TTCTGGCTGG CGGACTGTCC TCGGACTGCG 
GAGGCCGTGT CCTTTGCCAA CCTCCTGTAC CGCGAGCTGT CGGCCGTGCC GTACATGGCC 
AAGTTTGTAG TGTTTGCGAA GATGAATGAA GCTCGCGAGG GTCGACTCCG CTGCTACTGC 
ATGACGGATG ACAAGATGGA CAAAACCCTG GAGCAGCACG AGAACTTCTC GGAAGTGGCT 
CGCAGCAGAG ACATCGAGGT GATGGAGGGA ATGCCGCTGT ATTTGGAGTG TTCAGGAAAC 
TTGGTTCCTA TAAGAAAAGC AGCCCAGCAG CCTCGCTGTT TCAGCTTCCA GGCCTTTAAA 
GACAACAGAC TGCCTGTGTC CGTCAAGGTG AGGGACAGCA GTAAAGACCC CTCTGGGTTT 
TTGTCTTTCT TGCGCAAGTC AACCAAATAT GAAGACAGCC AACACGTGCT GTGCAACCTC 
AACGTGACCA TGCCGCCGTG CATTAAGATT ATGGGCAGCG ATGAGCGGAG GAGGACTCTG 
ACTCCTCTGG CTCTCAGAGA AAGATACAGC GCTCTGAATG AACCGGCAAT GGCATCACTG 
AGCGCCATGG AAAGGACGGA GTTAAAGATG GCTTTAATAG CTGAACAGCT AGGCCTGAGC 
TGGGCTGAGT TGGGACGGGA ACTGCAGTTT AATGTAGATG AAATAAATAA GATCCGTGTG 
GAGAATCCCA ACTCCCTTTT AGAGCAGAGC TCAACGCTGC TCAACCTGTG GGCCGCCCGA 
GAGGGGAAGA GAGCCAAAAT GGACAGCCTG TACGTGGCCC TCAAGAGCAT CGACCGAGTG 
GACATTGTGA ACATGATGGA GGGCCAGGGA GGACCACCGG CGGGTCAGCA GGGGGGCTGG 
GAGCAGGACG CTTCTAGGTC TAGGCATCAC GACCGAGACC ATCTCTCACC TGGAATCACA 
AATGGTTACG GGCTGGTGGT GCAGGAGGAA CTAGTGTCAC CGGCCTCCAT GCAGTACAGT 
CTGCCCTCGC CACTGGGCAA TGAGCCCTAC TGGCAGGAGG TTTCCAGCAT GGAGTGCGCG 
CCCATCGCCA CCACTGAAGA AGACACGCTC ATGGAGATGT CCGAGGTACA GGTTTGGCCC 
TCAGGAAATA GTCCGTCCAT TGTGGCTGTA GAGGACTCCT CATTGGAGTG CAGCAATGCG 
GACGACTCCG AGGCGCTGCA GAACCGGAGC CTGGGTCACT CCAGGAGCGG GACGCCCGGT 
GGGAACGGAC TCACTCCGGG AGGTTTAAGT GGCTCCATCG AGCTCCTGGA GGATCAGTCC 
ATCGGTGCGG ATTCAGACTA CAGTGCCGCC GGGATGCTGC GTCTGAGCGA AGGGGTCAAC 
ATCAGTGCTC TGGACAGAGG TCAGAGGTCG GAGAGTTCAG AAGGGGCTGT AGGTCACCAA 
GTCACCAACA ACATTAAAGA CGGTTCCAAT GGAGGTTTGG GAGAAGGAGG AGGTGACGGC 
ACACAGTTAG AAGATAGCAT ATCTCTCATT TCAGGGCAAC AGCGTGTGTA TGCTCGAATG 
AGCGAGTCGC CTGGGCTGAG AAGACGGGCA GATCCTGGCG AAGACAGGTT AAGCGCAGGG 
TCGTTCCTGT CTTATCTGCA GGATCAGGGC TCTCCTGGTT GGCATTCGGC TGCAGAAGCG 
ACTCAGAACA GATTGGCGTC TTTGCAATGC TCCCTTCCTA GGCAGGCAAT AGAGTCTATG 
ATGTCATCGG TGCGAGCTAC GGTGGACTCT GACTCCAAGC AGTCTTGTTT GGGCCAGGAG 
GCTCTGATTG AGCCAGTGCG GGACATGGGG CACTCTGAGA TCATCCATGG CCATTATCAG 
GGGACTCAGC CGTTTGAGAA AGGACTCGGC TTCCCGCACA GAACAACCAA CATGCGCACT 
TGGGAGGACA TGCGTCTGAG CGGACAGGGT GATGAGGCTG AGGATCTTCC AGGAGAGCAA 
GTGAGCGAGG AGCAATTCAC AGACGAGCAT GGCAACATCG TCACCAAAAA GGACTCGACT 
CCTTCACCCA AACCATCCTT CACTGACTCA TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( NM_001327890.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001314819.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant 1, mRNA.

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CloneID	ODa41278
Clone ID Related Accession (Same CDS sequence)	NM_001327890.1
Accession Version	NM_001327890.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5910bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-07-27
Organism	Danio rerio(zebrafish)
Product	ankyrin 1, erythrocytic a isoform 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from CABZ01065994.1, CABZ01065995.1, CABZ01065996.1 and CT033825.21. On May 27, 2016 this sequence version replaced XM_009302006.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCCAACCA ACACCATCCT TTTTGGAGCT GACGCTGCCA CCAGTTTCCT CCGCGCAGCT 
CGCTCAGGTA ATCTGGAAAA GGCTCTAGAC CACATCAAGA ACGGCATCGA CATCAACACA 
GCCAATCAGA ATGGACTCAA CGGGCTGCAT TTGGCCTCCA AGGAAGGCCA TGTTAAAATG 
GTGCTGGAGC TGCTTCACCA TGGTATTGTA CTTGAGACGA CAACGAAGAA AGGGAACACT 
GCTCTTCATA TAGCGGCGTT GGCTGGCCAG GAGCAGGTGG TCACTGAACT GGTCAACTAT 
GGAGCAAACG TCAACGCTCA GTCTCAGAAG GGCTTCACGC CGCTCTACAT GGCAGCACAA 
GAGAATCATC TGGAGGTTGT GAAGTTTCTG CTGGATAACG GAGCCAATCA GACCATTCCA 
ACTGAGGATG GCTTTACCCC TCTTGCTGTG GCTCTTCAGC AGGGCCATGA GAATGTGGTG 
GCCCTGCTCA TCAACCACGG TACTAAGGGC AAGGTCCGCC TTCCCGCACT GCACATCGCT 
GCCCGAAACG ACGACACCCG CACTGCCGCT GTGCTCTTAC AGAACGACCC CAATCCTGAC 
GTCCTGAGCA AGACAGGCTT CACGCCGCTC CACATCGCAG CACATTATGA AAACCTGAAT 
GTTGCTCAGC TTCTTCTGAA CCGAGGAGCT GATGTGAACT TCACACCTAA GAACGGGATC 
ACACCGCTGC ACATCGCATC CAGGAGAGGG AACGTCATCA TGGTCAGACT TCTGCTGGAC 
CGAGGAGCAA AGATCGATGC CAAGACAAAA GATGAGTTGA CTCCTCTTCA CTGTGCTGCC 
AGAAATGGCC ATGTGAGAAT CATCGAGATC CTTCTGGACC AAGGAGCCCC AATACAGGCC 
AAGACCAAAA ATGGCCTGTC GCCGATCCAC ATGGCAGCCC AGGGCGATCA CTTGGACTGT 
ATCCGACAGC TCTTACAGTA CAACGCAGAG ATCGATGACA TCACTCTGGA CCACCTGACA 
CCTCTGCATG TGGCGGCTCA CTGCGGGCAT CACCGCGTGG CCAAGGTGCT CCTGGATAAA 
GGAGCCAAAC CAAACACCCG CGCTCTGAAT GGATTCACGC CGCTCCACAT TGCCTGTAAG 
AAGAACCACA TGCGTGTAAT GGACCTGCTA TTAAAGCATT CTGCATCCCT GGAAGCTGTA 
ACCGAGTCAG GCTTGACCCC GCTGCATGTG TCCTCCTTCA TGGGTCATCT GAATATAGTC 
AAGATACTGA TGCAAAAAGG AGCTTCGCCG AACGCCTCAA ATGTGAAAGT GGAAACCCCT 
CTGCATATGG CATCTCGAGC TGGACACTGT GAAGTTGCTG AATTCCTATT GCAAAATGCA 
GCTCCAGTTG ATGCCAAAGC CAAGGATGAC CAGACGCCGC TACACTGTGC CTCCCGCATG 
GGCCACAACG AGATGGTGAA GTTGCTTCTG GAGCACAAGG CCAACTCAAA CTCCACCACC 
ACAGCAGGAC ACACACCGCT GCACATCGCG GCCCGCGAGG GACACACACA AACTGCCAGC 
ATCCTCCTGG ACATGAACGC CCAGCTCACC AAAATGACCA AGAAAGGCTT CACTCCTCTT 
CATGTTGCAG CCAAATACGG AAAGGTGGAC GTGGCAGTTC TGCTTCTGGA GAGGGGAGCA 
AATCCAAACG CTGCTGGAAA AGTTGGACTC ACGCCGCTGC ATGTGGCCGT CCATCATAAC 
AATCTAGATG TGGTCAATCT TTTACTCAGC AAAGGAGGAT CACCACACAG CGCCGCTCGG 
AATGGCTATA CTCCGCTCCA CATTGCTTCC AAACAGAACC AGGCGGAGGT GGCGAGCAGT 
TTACTGCAGC ACGGGGCGTC TGCCAATGCT GAGTCTCTGC AAGGGGTCAC ACCTCTACAC 
CTCGCCTCTC AAGAGGGTCA ACCTGACATG GTGTTACTGC TCATCTCCAA GCAGGCCAAT 
GTAAACCTTG GCAATAAGAG CGGTCTGACT CCTCTTCATC TTGTGGCTCA GGAAGGGCAT 
GTTGGGATTG CTGATATACT AGTGAAACAT GGAGCATCTG TTTATGCTGC CACACGGATG 
GGTTACACTC CGCTTCACGT GGCTTGTCAT TATGGGAATA TCAAGATGGT GAAGTTTCTC 
TTACAGCAGC AGGCCAACGT AAACTGCAAG ACCAGGATGA GCTACACGCC GCTACATCAG 
GCTGCTCAGC AGGGTCATAC GGACATCGTG ACGCTTCTGC TGAAACACGG AGCACAACCG 
AATGAAGTTA CATCTAACGG CACCTCTGCT CTTGCCATCG CCAAGCGGCT TGGATACATT 
TCAGTCATCG ATGTGCTCAA ACTGGTCACT GAAGAGACGG TCACTATGAC CACCACTGAA 
AAACATCGCA TGAGTTTCCC TGAAACCGTT GATGAGATCC TGGATGTGTC TGAAGATGAA 
GGAATTGCAC AGCTAACATT AGGCGAGGAG CTGCTGGGAA ACGAGGGCGC CAGGTATTTG 
ATGAAGATGG AGGACATAAA GGACCATGAT GAGGATTTCC TTTCCCCCAA GAAAGTTCTA 
GAGTACGAGG GAGAGTTGGA GAATCATTCT CCTGCTATCC CGAGGATCCC GTGTGTGTCT 
CCTGAGACTG TCATGCTGAA AGAGCATGAG ATGGAGCAGC AGCACACTCC ACTTCCATTG 
CCCAAAGAGT ACGACGAGGA TTCTCTGATT CCCAGCAGTC CTGCAACTGA GACTTCAGAC 
AATGTCAGTC CGGTTGCCAG TCCCATCCAC ACCGGTTTTC TGGTGAGCTT CATGGTGGAT 
GCTCGCGGCG GCTCGATGCG CGGCAGCAGA CACAACGGCC TCCGCGTCAT CATTCCTCCT 
CGAACCTGCG CCGCTCCCAC ACGCATCACC TGCCGCCTGG TGAAGCCCCA GAAGCTGCCC 
ACACCGCCGC CATTAGTGGA GGGAGAGGGT CTCGCCAGCC GCATCATCTC CCTTGGACCC 
GCCAGCATGC AGTTTTTAGG GCCTGTGATT GTGGAGATTC CTCATTTTGC GGCTCTCGGT 
CGGGGAGATC GGGAACTCGT GGTCTTGAGG AGTGAGAACG GATCGGTCTG GAAGGAACAT 
CGTAACCGAT ATGGAGATGA AGTGCTAGAG ACCATCCTCA ACGGCATGGA TGAAGATCTG 
GAGAGTCAGG AGGAGCTCGG GAAGAAACGC ATCAGACGGA TCATCTCCAC CGACTTCCCG 
CTCTACTTTG CGGTGGTGTC TCGCATCCAG CAGGAGAACG ATCTGATTGG CCCAGAAGGA 
GGTTTTCTGT CCAGTAAACT GGTGCCGATG GTCCAGGCCA CCTTTCCAGA GACAGCCGTC 
ACCAAACGCG TGCGTCTGGG ACTGCAGGCC CAGCCGGTGC CTGATGAGCT GGTGGCCAAG 
CTTTTGGGGA ACCAGGCCAC GTTCAGTCCT GTGGTGACGG TGGAGCCTCG GCGCCGGAAG 
TTTCACCGTC CCATCGGCCT AAGAATCCCA CTGCCACCCT CATGGAGAGA GAGCCCGCGG 
GACGCCGGAG AGGGAGACAC CACCAGCCTG CGCCTGCTCT GCAGCGTCAT CGGTGGAACA 
GCTCCTGCCC AGTGGGAAGA TATAACTGGC ACCACCAAAC TGGCGTATTC CAATGACTGT 
GCCAGTTTCA CCACCAACGT CTCTGCCCGA TTCTGGCTGG CGGACTGTCC TCGGACTGCG 
GAGGCCGTGT CCTTTGCCAA CCTCCTGTAC CGCGAGCTGT CGGCCGTGCC GTACATGGCC 
AAGTTTGTAG TGTTTGCGAA GATGAATGAA GCTCGCGAGG GTCGACTCCG CTGCTACTGC 
ATGACGGATG ACAAGATGGA CAAAACCCTG GAGCAGCACG AGAACTTCTC GGAAGTGGCT 
CGCAGCAGAG ACATCGAGGT GATGGAGGGA ATGCCGCTGT ATTTGGAGTG TTCAGGAAAC 
TTGGTTCCTA TAAGAAAAGC AGCCCAGCAG CCTCGCTGTT TCAGCTTCCA GGCCTTTAAA 
GACAACAGAC TGCCTGTGTC CGTCAAGGTG AGGGACAGCA GTAAAGACCC CTCTGGGTTT 
TTGTCTTTCT TGCGCAAGTC AACCAAATAT GAAGACAGCC AACACGTGCT GTGCAACCTC 
AACGTGACCA TGCCGCCGTG CATTAAGATT ATGGGCAGCG ATGAGCGGAG GAGGACTCTG 
ACTCCTCTGG CTCTCAGAGA AAGATACAGC GCTCTGAATG AACCGGCAAT GGCATCACTG 
AGCGCCATGG AAAGGACGGA GTTAAAGATG GCTTTAATAG CTGAACAGCT AGGCCTGAGC 
TGGGCTGAGT TGGGACGGGA ACTGCAGTTT AATGTAGATG AAATAAATAA GATCCGTGTG 
GAGAATCCCA ACTCCCTTTT AGAGCAGAGC TCAACGCTGC TCAACCTGTG GGCCGCCCGA 
GAGGGGAAGA GAGCCAAAAT GGACAGCCTG TACGTGGCCC TCAAGAGCAT CGACCGAGTG 
GACATTGTGA ACATGATGGA GGGCCAGGGA GGACCACCGG CGGGTCAGCA GGGGGGCTGG 
GAGCAGGACG CTTCTAGGTC TAGGCATCAC GACCGAGACC ATCTCTCACC TGGAATCACA 
AATGGTTACG GGCTGGTGGT GCAGGAGGAA CTAGTGTCAC CGGCCTCCAT GCAGTACAGT 
CTGCCCTCGC CACTGGGCAA TGAGCCCTAC TGGCAGGAGG TTTCCAGCAT GGAGTGCGCG 
CCCATCGCCA CCACTGAAGA AGACACGCTC ATGGAGATGT CCGAGGTACA GGTTTGGCCC 
TCAGGAAATA GTCCGTCCAT TGTGGCTGTA GAGGACTCCT CATTGGAGTG CAGCAATGCG 
GACGACTCCG AGGCGCTGCA GAACCGGAGC CTGGGTCACT CCAGGAGCGG GACGCCCGGT 
GGGAACGGAC TCACTCCGGG AGGTTTAAGT GGCTCCATCG AGCTCCTGGA GGATCAGTCC 
ATCGGTGCGG ATTCAGACTA CAGTGCCGCC GGGATGCTGC GTCTGAGCGA AGGGGTCAAC 
ATCAGTGCTC TGGACAGAGG TCAGAGGTCG GAGAGTTCAG AAGGGGCTGT AGGTCACCAA 
GTCACCAACA ACATTAAAGA CGGTTCCAAT GGAGGTTTGG GAGAAGGAGG AGGTGACGGC 
ACACAGTTAG AAGATAGCAT ATCTCTCATT TCAGGGCAAC AGCGTGTGTA TGCTCGAATG 
AGCGAGTCGC CTGGGCTGAG AAGACGGGCA GATCCTGGCG AAGACAGGTT AAGCGCAGGG 
TCGTTCCTGT CTTATCTGCA GGATCAGGGC TCTCCTGGTT GGCATTCGGC TGCAGAAGCG 
ACTCAGAACA GATTGGCGTC TTTGCAATGC TCCCTTCCTA GGCAGGCAAT AGAGTCTATG 
ATGTCATCGG TGCGAGCTAC GGTGGACTCT GACTCCAAGC AGTCTTGTTT GGGCCAGGAG 
GCTCTGATTG AGCCAGTGCG GGACATGGGG CACTCTGAGA TCATCCATGG CCATTATCAG 
GGGACTCAGC CGTTTGAGAA AGGACTCGGC TTCCCGCACA GAACAACCAA CATGCGCACT 
TGGGAGGACA TGCGTCTGAG CGGACAGGGT GATGAGGCTG AGGATCTTCC AGGAGAGCAA 
GTGAGCGAGG AGCAATTCAC AGACGAGCAT GGCAACATCG TCACCAAAAA GATTGTGAGG 
AAAGTGGTCC GGAGAGGAAA AGGGGAGGAA GGGGGTCAGG AGCTGATCAT TGAAGGTCTG 
CAGCAGGATA TCAGTGAGCC TGACTTTGAT GGAGAACAGT TCATGAGCTA TGCCGTCCTG 
GGACGTGACA GCAAGCCGGA TGTTGTGGAT GTGAAGAGAG GAGGTGCTCA GATAGTCAAA 
TGTGCGAGTC TGCGGAGAGT AAAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001314819.1
CDS	70..5979
Translation	MPTNTILFGADAATSFLRAARSGNLEKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLINHGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGLTPLHVSSFMGHLNIVKILMQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGANPNAAGKVGLTPLHVAVHHNNLDVVNLLLSKGGSPHSAARNGYTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPLHLASQEGQPDMVLLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKHGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSNGTSALAIAKRLGYISVIDVLKLVTEETVTMTTTEKHRMSFPETVDEILDVSEDEGIAQLTLGEELLGNEGARYLMKMEDIKDHDEDFLSPKKVLEYEGELENHSPAIPRIPCVSPETVMLKEHEMEQQHTPLPLPKEYDEDSLIPSSPATETSDNVSPVASPIHTGFLVSFMVDARGGSMRGSRHNGLRVIIPPRTCAAPTRITCRLVKPQKLPTPPPLVEGEGLASRIISLGPASMQFLGPVIVEIPHFAALGRGDRELVVLRSENGSVWKEHRNRYGDEVLETILNGMDEDLESQEELGKKRIRRIISTDFPLYFAVVSRIQQENDLIGPEGGFLSSKLVPMVQATFPETAVTKRVRLGLQAQPVPDELVAKLLGNQATFSPVVTVEPRRRKFHRPIGLRIPLPPSWRESPRDAGEGDTTSLRLLCSVIGGTAPAQWEDITGTTKLAYSNDCASFTTNVSARFWLADCPRTAEAVSFANLLYRELSAVPYMAKFVVFAKMNEAREGRLRCYCMTDDKMDKTLEQHENFSEVARSRDIEVMEGMPLYLECSGNLVPIRKAAQQPRCFSFQAFKDNRLPVSVKVRDSSKDPSGFLSFLRKSTKYEDSQHVLCNLNVTMPPCIKIMGSDERRRTLTPLALRERYSALNEPAMASLSAMERTELKMALIAEQLGLSWAELGRELQFNVDEINKIRVENPNSLLEQSSTLLNLWAAREGKRAKMDSLYVALKSIDRVDIVNMMEGQGGPPAGQQGGWEQDASRSRHHDRDHLSPGITNGYGLVVQEELVSPASMQYSLPSPLGNEPYWQEVSSMECAPIATTEEDTLMEMSEVQVWPSGNSPSIVAVEDSSLECSNADDSEALQNRSLGHSRSGTPGGNGLTPGGLSGSIELLEDQSIGADSDYSAAGMLRLSEGVNISALDRGQRSESSEGAVGHQVTNNIKDGSNGGLGEGGGDGTQLEDSISLISGQQRVYARMSESPGLRRRADPGEDRLSAGSFLSYLQDQGSPGWHSAAEATQNRLASLQCSLPRQAIESMMSSVRATVDSDSKQSCLGQEALIEPVRDMGHSEIIHGHYQGTQPFEKGLGFPHRTTNMRTWEDMRLSGQGDEAEDLPGEQVSEEQFTDEHGNIVTKKIVRKVVRRGKGEEGGQELIIEGLQQDISEPDFDGEQFMSYAVLGRDSKPDVVDVKRGGAQIVKCASLRRVKQ

Target ORF information:

RefSeq Version	NM_001327890.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant 1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001327890.1

ORF Insert Sequence:

ATGCCAACCA ACACCATCCT TTTTGGAGCT GACGCTGCCA CCAGTTTCCT CCGCGCAGCT 
CGCTCAGGTA ATCTGGAAAA GGCTCTAGAC CACATCAAGA ACGGCATCGA CATCAACACA 
GCCAATCAGA ATGGACTCAA CGGGCTGCAT TTGGCCTCCA AGGAAGGCCA TGTTAAAATG 
GTGCTGGAGC TGCTTCACCA TGGTATTGTA CTTGAGACGA CAACGAAGAA AGGGAACACT 
GCTCTTCATA TAGCGGCGTT GGCTGGCCAG GAGCAGGTGG TCACTGAACT GGTCAACTAT 
GGAGCAAACG TCAACGCTCA GTCTCAGAAG GGCTTCACGC CGCTCTACAT GGCAGCACAA 
GAGAATCATC TGGAGGTTGT GAAGTTTCTG CTGGATAACG GAGCCAATCA GACCATTCCA 
ACTGAGGATG GCTTTACCCC TCTTGCTGTG GCTCTTCAGC AGGGCCATGA GAATGTGGTG 
GCCCTGCTCA TCAACCACGG TACTAAGGGC AAGGTCCGCC TTCCCGCACT GCACATCGCT 
GCCCGAAACG ACGACACCCG CACTGCCGCT GTGCTCTTAC AGAACGACCC CAATCCTGAC 
GTCCTGAGCA AGACAGGCTT CACGCCGCTC CACATCGCAG CACATTATGA AAACCTGAAT 
GTTGCTCAGC TTCTTCTGAA CCGAGGAGCT GATGTGAACT TCACACCTAA GAACGGGATC 
ACACCGCTGC ACATCGCATC CAGGAGAGGG AACGTCATCA TGGTCAGACT TCTGCTGGAC 
CGAGGAGCAA AGATCGATGC CAAGACAAAA GATGAGTTGA CTCCTCTTCA CTGTGCTGCC 
AGAAATGGCC ATGTGAGAAT CATCGAGATC CTTCTGGACC AAGGAGCCCC AATACAGGCC 
AAGACCAAAA ATGGCCTGTC GCCGATCCAC ATGGCAGCCC AGGGCGATCA CTTGGACTGT 
ATCCGACAGC TCTTACAGTA CAACGCAGAG ATCGATGACA TCACTCTGGA CCACCTGACA 
CCTCTGCATG TGGCGGCTCA CTGCGGGCAT CACCGCGTGG CCAAGGTGCT CCTGGATAAA 
GGAGCCAAAC CAAACACCCG CGCTCTGAAT GGATTCACGC CGCTCCACAT TGCCTGTAAG 
AAGAACCACA TGCGTGTAAT GGACCTGCTA TTAAAGCATT CTGCATCCCT GGAAGCTGTA 
ACCGAGTCAG GCTTGACCCC GCTGCATGTG TCCTCCTTCA TGGGTCATCT GAATATAGTC 
AAGATACTGA TGCAAAAAGG AGCTTCGCCG AACGCCTCAA ATGTGAAAGT GGAAACCCCT 
CTGCATATGG CATCTCGAGC TGGACACTGT GAAGTTGCTG AATTCCTATT GCAAAATGCA 
GCTCCAGTTG ATGCCAAAGC CAAGGATGAC CAGACGCCGC TACACTGTGC CTCCCGCATG 
GGCCACAACG AGATGGTGAA GTTGCTTCTG GAGCACAAGG CCAACTCAAA CTCCACCACC 
ACAGCAGGAC ACACACCGCT GCACATCGCG GCCCGCGAGG GACACACACA AACTGCCAGC 
ATCCTCCTGG ACATGAACGC CCAGCTCACC AAAATGACCA AGAAAGGCTT CACTCCTCTT 
CATGTTGCAG CCAAATACGG AAAGGTGGAC GTGGCAGTTC TGCTTCTGGA GAGGGGAGCA 
AATCCAAACG CTGCTGGAAA AGTTGGACTC ACGCCGCTGC ATGTGGCCGT CCATCATAAC 
AATCTAGATG TGGTCAATCT TTTACTCAGC AAAGGAGGAT CACCACACAG CGCCGCTCGG 
AATGGCTATA CTCCGCTCCA CATTGCTTCC AAACAGAACC AGGCGGAGGT GGCGAGCAGT 
TTACTGCAGC ACGGGGCGTC TGCCAATGCT GAGTCTCTGC AAGGGGTCAC ACCTCTACAC 
CTCGCCTCTC AAGAGGGTCA ACCTGACATG GTGTTACTGC TCATCTCCAA GCAGGCCAAT 
GTAAACCTTG GCAATAAGAG CGGTCTGACT CCTCTTCATC TTGTGGCTCA GGAAGGGCAT 
GTTGGGATTG CTGATATACT AGTGAAACAT GGAGCATCTG TTTATGCTGC CACACGGATG 
GGTTACACTC CGCTTCACGT GGCTTGTCAT TATGGGAATA TCAAGATGGT GAAGTTTCTC 
TTACAGCAGC AGGCCAACGT AAACTGCAAG ACCAGGATGA GCTACACGCC GCTACATCAG 
GCTGCTCAGC AGGGTCATAC GGACATCGTG ACGCTTCTGC TGAAACACGG AGCACAACCG 
AATGAAGTTA CATCTAACGG CACCTCTGCT CTTGCCATCG CCAAGCGGCT TGGATACATT 
TCAGTCATCG ATGTGCTCAA ACTGGTCACT GAAGAGACGG TCACTATGAC CACCACTGAA 
AAACATCGCA TGAGTTTCCC TGAAACCGTT GATGAGATCC TGGATGTGTC TGAAGATGAA 
GGAATTGCAC AGCTAACATT AGGCGAGGAG CTGCTGGGAA ACGAGGGCGC CAGGTATTTG 
ATGAAGATGG AGGACATAAA GGACCATGAT GAGGATTTCC TTTCCCCCAA GAAAGTTCTA 
GAGTACGAGG GAGAGTTGGA GAATCATTCT CCTGCTATCC CGAGGATCCC GTGTGTGTCT 
CCTGAGACTG TCATGCTGAA AGAGCATGAG ATGGAGCAGC AGCACACTCC ACTTCCATTG 
CCCAAAGAGT ACGACGAGGA TTCTCTGATT CCCAGCAGTC CTGCAACTGA GACTTCAGAC 
AATGTCAGTC CGGTTGCCAG TCCCATCCAC ACCGGTTTTC TGGTGAGCTT CATGGTGGAT 
GCTCGCGGCG GCTCGATGCG CGGCAGCAGA CACAACGGCC TCCGCGTCAT CATTCCTCCT 
CGAACCTGCG CCGCTCCCAC ACGCATCACC TGCCGCCTGG TGAAGCCCCA GAAGCTGCCC 
ACACCGCCGC CATTAGTGGA GGGAGAGGGT CTCGCCAGCC GCATCATCTC CCTTGGACCC 
GCCAGCATGC AGTTTTTAGG GCCTGTGATT GTGGAGATTC CTCATTTTGC GGCTCTCGGT 
CGGGGAGATC GGGAACTCGT GGTCTTGAGG AGTGAGAACG GATCGGTCTG GAAGGAACAT 
CGTAACCGAT ATGGAGATGA AGTGCTAGAG ACCATCCTCA ACGGCATGGA TGAAGATCTG 
GAGAGTCAGG AGGAGCTCGG GAAGAAACGC ATCAGACGGA TCATCTCCAC CGACTTCCCG 
CTCTACTTTG CGGTGGTGTC TCGCATCCAG CAGGAGAACG ATCTGATTGG CCCAGAAGGA 
GGTTTTCTGT CCAGTAAACT GGTGCCGATG GTCCAGGCCA CCTTTCCAGA GACAGCCGTC 
ACCAAACGCG TGCGTCTGGG ACTGCAGGCC CAGCCGGTGC CTGATGAGCT GGTGGCCAAG 
CTTTTGGGGA ACCAGGCCAC GTTCAGTCCT GTGGTGACGG TGGAGCCTCG GCGCCGGAAG 
TTTCACCGTC CCATCGGCCT AAGAATCCCA CTGCCACCCT CATGGAGAGA GAGCCCGCGG 
GACGCCGGAG AGGGAGACAC CACCAGCCTG CGCCTGCTCT GCAGCGTCAT CGGTGGAACA 
GCTCCTGCCC AGTGGGAAGA TATAACTGGC ACCACCAAAC TGGCGTATTC CAATGACTGT 
GCCAGTTTCA CCACCAACGT CTCTGCCCGA TTCTGGCTGG CGGACTGTCC TCGGACTGCG 
GAGGCCGTGT CCTTTGCCAA CCTCCTGTAC CGCGAGCTGT CGGCCGTGCC GTACATGGCC 
AAGTTTGTAG TGTTTGCGAA GATGAATGAA GCTCGCGAGG GTCGACTCCG CTGCTACTGC 
ATGACGGATG ACAAGATGGA CAAAACCCTG GAGCAGCACG AGAACTTCTC GGAAGTGGCT 
CGCAGCAGAG ACATCGAGGT GATGGAGGGA ATGCCGCTGT ATTTGGAGTG TTCAGGAAAC 
TTGGTTCCTA TAAGAAAAGC AGCCCAGCAG CCTCGCTGTT TCAGCTTCCA GGCCTTTAAA 
GACAACAGAC TGCCTGTGTC CGTCAAGGTG AGGGACAGCA GTAAAGACCC CTCTGGGTTT 
TTGTCTTTCT TGCGCAAGTC AACCAAATAT GAAGACAGCC AACACGTGCT GTGCAACCTC 
AACGTGACCA TGCCGCCGTG CATTAAGATT ATGGGCAGCG ATGAGCGGAG GAGGACTCTG 
ACTCCTCTGG CTCTCAGAGA AAGATACAGC GCTCTGAATG AACCGGCAAT GGCATCACTG 
AGCGCCATGG AAAGGACGGA GTTAAAGATG GCTTTAATAG CTGAACAGCT AGGCCTGAGC 
TGGGCTGAGT TGGGACGGGA ACTGCAGTTT AATGTAGATG AAATAAATAA GATCCGTGTG 
GAGAATCCCA ACTCCCTTTT AGAGCAGAGC TCAACGCTGC TCAACCTGTG GGCCGCCCGA 
GAGGGGAAGA GAGCCAAAAT GGACAGCCTG TACGTGGCCC TCAAGAGCAT CGACCGAGTG 
GACATTGTGA ACATGATGGA GGGCCAGGGA GGACCACCGG CGGGTCAGCA GGGGGGCTGG 
GAGCAGGACG CTTCTAGGTC TAGGCATCAC GACCGAGACC ATCTCTCACC TGGAATCACA 
AATGGTTACG GGCTGGTGGT GCAGGAGGAA CTAGTGTCAC CGGCCTCCAT GCAGTACAGT 
CTGCCCTCGC CACTGGGCAA TGAGCCCTAC TGGCAGGAGG TTTCCAGCAT GGAGTGCGCG 
CCCATCGCCA CCACTGAAGA AGACACGCTC ATGGAGATGT CCGAGGTACA GGTTTGGCCC 
TCAGGAAATA GTCCGTCCAT TGTGGCTGTA GAGGACTCCT CATTGGAGTG CAGCAATGCG 
GACGACTCCG AGGCGCTGCA GAACCGGAGC CTGGGTCACT CCAGGAGCGG GACGCCCGGT 
GGGAACGGAC TCACTCCGGG AGGTTTAAGT GGCTCCATCG AGCTCCTGGA GGATCAGTCC 
ATCGGTGCGG ATTCAGACTA CAGTGCCGCC GGGATGCTGC GTCTGAGCGA AGGGGTCAAC 
ATCAGTGCTC TGGACAGAGG TCAGAGGTCG GAGAGTTCAG AAGGGGCTGT AGGTCACCAA 
GTCACCAACA ACATTAAAGA CGGTTCCAAT GGAGGTTTGG GAGAAGGAGG AGGTGACGGC 
ACACAGTTAG AAGATAGCAT ATCTCTCATT TCAGGGCAAC AGCGTGTGTA TGCTCGAATG 
AGCGAGTCGC CTGGGCTGAG AAGACGGGCA GATCCTGGCG AAGACAGGTT AAGCGCAGGG 
TCGTTCCTGT CTTATCTGCA GGATCAGGGC TCTCCTGGTT GGCATTCGGC TGCAGAAGCG 
ACTCAGAACA GATTGGCGTC TTTGCAATGC TCCCTTCCTA GGCAGGCAAT AGAGTCTATG 
ATGTCATCGG TGCGAGCTAC GGTGGACTCT GACTCCAAGC AGTCTTGTTT GGGCCAGGAG 
GCTCTGATTG AGCCAGTGCG GGACATGGGG CACTCTGAGA TCATCCATGG CCATTATCAG 
GGGACTCAGC CGTTTGAGAA AGGACTCGGC TTCCCGCACA GAACAACCAA CATGCGCACT 
TGGGAGGACA TGCGTCTGAG CGGACAGGGT GATGAGGCTG AGGATCTTCC AGGAGAGCAA 
GTGAGCGAGG AGCAATTCAC AGACGAGCAT GGCAACATCG TCACCAAAAA GATTGTGAGG 
AAAGTGGTCC GGAGAGGAAA AGGGGAGGAA GGGGGTCAGG AGCTGATCAT TGAAGGTCTG 
CAGCAGGATA TCAGTGAGCC TGACTTTGAT GGAGAACAGT TCATGAGCTA TGCCGTCCTG 
GGACGTGACA GCAAGCCGGA TGTTGTGGAT GTGAAGAGAG GAGGTGCTCA GATAGTCAAA 
TGTGCGAGTC TGCGGAGAGT AAAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_009302007.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009300282.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X1, mRNA.

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CloneID	ODa41279
Clone ID Related Accession (Same CDS sequence)	XM_009302007.2
Accession Version	XM_009302007.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ankyrin 1, erythrocytic a isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302007.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAACCA ACACCATCCT TTTTGGAGCT GACGCTGCCA CCAGTTTCCT CCGCGCAGCT 
CGCTCAGGTA ATCTGGAAAA GGCTCTAGAC CACATCAAGA ACGGCATCGA CATCAACACA 
GCCAATCAGA ATGGACTCAA CGGGCTGCAT TTGGCCTCCA AGGAAGGCCA TGTTAAAATG 
GTGCTGGAGC TGCTTCACCA TGGTATTGTA CTTGAGACGA CAACGAAGAA AGGGAACACT 
GCTCTTCATA TAGCGGCGTT GGCTGGCCAG GAGCAGGTGG TCACTGAACT GGTCAACTAT 
GGAGCAAACG TCAACGCTCA GTCTCAGAAG GGCTTCACGC CGCTCTACAT GGCAGCACAA 
GAGAATCATC TGGAGGTTGT GAAGTTTCTG CTGGATAACG GAGCCAATCA GACCATTCCA 
ACTGAGGATG GCTTTACCCC TCTTGCTGTG GCTCTTCAGC AGGGCCATGA GAATGTGGTG 
GCCCTGCTCA TCAACCACGG TACTAAGGGC AAGGTCCGCC TTCCCGCACT GCACATCGCT 
GCCCGAAACG ACGACACCCG CACTGCCGCT GTGCTCTTAC AGAACGACCC CAATCCTGAC 
GTCCTGAGCA AGACAGGCTT CACGCCGCTC CACATCGCAG CACATTATGA AAACCTGAAT 
GTTGCTCAGC TTCTTCTGAA CCGAGGAGCT GATGTGAACT TCACACCTAA GAACGGGATC 
ACACCGCTGC ACATCGCATC CAGGAGAGGG AACGTCATCA TGGTCAGACT TCTGCTGGAC 
CGAGGAGCAA AGATCGATGC CAAGACAAAA GATGAGTTGA CTCCTCTTCA CTGTGCTGCC 
AGAAATGGCC ATGTGAGAAT CATCGAGATC CTTCTGGACC AAGGAGCCCC AATACAGGCC 
AAGACCAAAA ATGGCCTGTC GCCGATCCAC ATGGCAGCCC AGGGCGATCA CTTGGACTGT 
ATCCGACAGC TCTTACAGTA CAACGCAGAG ATCGATGACA TCACTCTGGA CCACCTGACA 
CCTCTGCATG TGGCGGCTCA CTGCGGGCAT CACCGCGTGG CCAAGGTGCT CCTGGATAAA 
GGAGCCAAAC CAAACACCCG CGCTCTGAAT GGATTCACGC CGCTCCACAT TGCCTGTAAG 
AAGAACCACA TGCGTGTAAT GGACCTGCTA TTAAAGCATT CTGCATCCCT GGAAGCTGTA 
ACCGAGTCAG GCTTGACCCC GCTGCATGTG TCCTCCTTCA TGGGTCATCT GAATATAGTC 
AAGATACTGA TGCAAAAAGG AGCTTCGCCG AACGCCTCAA ATGTGAAAGT GGAAACCCCT 
CTGCATATGG CATCTCGAGC TGGACACTGT GAAGTTGCTG AATTCCTATT GCAAAATGCA 
GCTCCAGTTG ATGCCAAAGC CAAGGATGAC CAGACGCCGC TACACTGTGC CTCCCGCATG 
GGCCACAACG AGATGGTGAA GTTGCTTCTG GAGCACAAGG CCAACTCAAA CTCCACCACC 
ACAGCAGGAC ACACACCGCT GCACATCGCG GCCCGCGAGG GACACACACA AACTGCCAGC 
ATCCTCCTGG ACATGAACGC CCAGCTCACC AAAATGACCA AGAAAGGCTT CACTCCTCTT 
CATGTTGCAG CCAAATACGG AAAGGTGGAC GTGGCAGTTC TGCTTCTGGA GAGGGGAGCA 
AATCCAAACG CTGCTGGAAA AGTTGGACTC ACGCCGCTGC ATGTGGCCGT CCATCATAAC 
AATCTAGATG TGGTCAATCT TTTACTCAGC AAAGGAGGAT CACCACACAG CGCCGCTCGG 
AATGGCTATA CTCCGCTCCA CATTGCTTCC AAACAGAACC AGGCGGAGGT GGCGAGCAGT 
TTACTGCAGC ACGGGGCGTC TGCCAATGCT GAGTCTCTGC AAGGGGTCAC ACCTCTACAC 
CTCGCCTCTC AAGAGGGTCA ACCTGACATG GTGTTACTGC TCATCTCCAA GCAGGCCAAT 
GTAAACCTTG GCAATAAGAG CGGTCTGACT CCTCTTCATC TTGTGGCTCA GGAAGGGCAT 
GTTGGGATTG CTGATATACT AGTGAAACAT GGAGCATCTG TTTATGCTGC CACACGGATG 
GGTTACACTC CGCTTCACGT GGCTTGTCAT TATGGGAATA TCAAGATGGT GAAGTTTCTC 
TTACAGCAGC AGGCCAACGT AAACTGCAAG ACCAGGATGA GCTACACGCC GCTACATCAG 
GCTGCTCAGC AGGGTCATAC GGACATCGTG ACGCTTCTGC TGAAACACGG AGCACAACCG 
AATGAAGTTA CATCTAACGG CACCTCTGCT CTTGCCATCG CCAAGCGGCT TGGATACATT 
TCAGTCATCG ATGTGCTCAA ACTGGTCACT GAAGAGACGG TCACTATGAC CACCACTGAA 
AAACATCGCA TGAGTTTCCC TGAAACCGTT GATGAGATCC TGGATGTGTC TGAAGATGAA 
GGAATTGCAC AGCTAACATT AGGCGAGGAG CTGCTGGGAA ACGAGGGCGC CAGGTATTTG 
ATGAAGATGG AGGACATAAA GGACCATGAT GAGGATTTCC TTTCCCCCAA GAAAGTTCTA 
GAGTACGAGG GAGAGTTGGA GAATCATTCT CCTGCTATCC CGAGGATCCC GTGTGTGTCT 
CCTGAGACTG TCATGCTGAA AGAGCATGAG ATGGAGCAGC ACACTCCACT TCCATTGCCC 
AAAGAGTACG ACGAGGATTC TCTGATTCCC AGCAGTCCTG CAACTGAGAC TTCAGACAAT 
GTCAGTCCGG TTGCCAGTCC CATCCACACC GGTTTTCTGG TGAGCTTCAT GGTGGATGCT 
CGCGGCGGCT CGATGCGCGG CAGCAGACAC AACGGCCTCC GCGTCATCAT TCCTCCTCGA 
ACCTGCGCCG CTCCCACACG CATCACCTGC CGCCTGGTGA AGCCCCAGAA GCTGCCCACA 
CCGCCGCCAT TAGTGGAGGG AGAGGGTCTC GCCAGCCGCA TCATCTCCCT TGGACCCGCC 
AGCATGCAGT TTTTAGGGCC TGTGATTGTG GAGATTCCTC ATTTTGCGGC TCTCGGTCGG 
GGAGATCGGG AACTCGTGGT CTTGAGGAGT GAGAACGGAT CGGTCTGGAA GGAACATCGT 
AACCGATATG GAGATGAAGT GCTAGAGACC ATCCTCAACG GCATGGATGA AGATCTGGAG 
AGTCAGGAGG AGCTCGGGAA GAAACGCATC AGACGGATCA TCTCCACCGA CTTCCCGCTC 
TACTTTGCGG TGGTGTCTCG CATCCAGCAG GAGAACGATC TGATTGGCCC AGAAGGAGGT 
TTTCTGTCCA GTAAACTGGT GCCGATGGTC CAGGCCACCT TTCCAGAGAC AGCCGTCACC 
AAACGCGTGC GTCTGGGACT GCAGGCCCAG CCGGTGCCTG ATGAGCTGGT GGCCAAGCTT 
TTGGGGAACC AGGCCACGTT CAGTCCTGTG GTGACGGTGG AGCCTCGGCG CCGGAAGTTT 
CACCGTCCCA TCGGCCTAAG AATCCCACTG CCACCCTCAT GGAGAGAGAG CCCGCGGGAC 
GCCGGAGAGG GAGACACCAC CAGCCTGCGC CTGCTCTGCA GCGTCATCGG TGGAACAGCT 
CCTGCCCAGT GGGAAGATAT AACTGGCACC ACCAAACTGG CGTATTCCAA TGACTGTGCC 
AGTTTCACCA CCAACGTCTC TGCCCGATTC TGGCTGGCGG ACTGTCCTCG GACTGCGGAG 
GCCGTGTCCT TTGCCAACCT CCTGTACCGC GAGCTGTCGG CCGTGCCGTA CATGGCCAAG 
TTTGTAGTGT TTGCGAAGAT GAATGAAGCT CGCGAGGGTC GACTCCGCTG CTACTGCATG 
ACGGATGACA AGATGGACAA AACCCTGGAG CAGCACGAGA ACTTCTCGGA AGTGGCTCGC 
AGCAGAGACA TCGAGGTGAT GGAGGGAATG CCGCTGTATT TGGAGTGTTC AGGAAACTTG 
GTTCCTATAA GAAAAGCAGC CCAGCAGCCT CGCTGTTTCA GCTTCCAGGC CTTTAAAGAC 
AACAGACTGC CTGTGTCCGT CAAGGTGAGG GACAGCAGTA AAGACCCCTC TGGGTTTTTG 
TCTTTCTTGC GCAAGTCAAC CAAATATGAA GACAGCCAAC ACGTGCTGTG CAACCTCAAC 
GTGACCATGC CGCCGTGCAT TAAGATTATG GGCAGCGATG AGCGGAGGAG GACTCTGACT 
CCTCTGGCTC TCAGAGAAAG ATACAGCGCT CTGAATGAAC CGGCAATGGC ATCACTGAGC 
GCCATGGAAA GGACGGAGTT AAAGATGGCT TTAATAGCTG AACAGCTAGG CCTGAGCTGG 
GCTGAGTTGG GACGGGAACT GCAGTTTAAT GTAGATGAAA TAAATAAGAT CCGTGTGGAG 
AATCCCAACT CCCTTTTAGA GCAGAGCTCA ACGCTGCTCA ACCTGTGGGC CGCCCGAGAG 
GGGAAGAGAG CCAAAATGGA CAGCCTGTAC GTGGCCCTCA AGAGCATCGA CCGAGTGGAC 
ATTGTGAACA TGATGGAGGG CCAGGGAGGA CCACCGGCGG GTCAGCAGGG GGGCTGGGAG 
CAGGACGCTT CTAGGTCTAG GCATCACGAC CGAGACCATC TCTCACCTGG AATCACAAAT 
GGTTACGGGC TGGTGGTGCA GGAGGAACTA GTGTCACCGG CCTCCATGCA GTACAGTCTG 
CCCTCGCCAC TGGGCAATGA GCCCTACTGG CAGGAGGTTT CCAGCATGGA GTGCGCGCCC 
ATCGCCACCA CTGAAGAAGA CACGCTCATG GAGATGTCCG AGGTACAGGT TTGGCCCTCA 
GGAAATAGTC CGTCCATTGT GGCTGTAGAG GACTCCTCAT TGGAGTGCAG CAATGCGGAC 
GACTCCGAGG CGCTGCAGAA CCGGAGCCTG GGTCACTCCA GGAGCGGGAC GCCCGGTGGG 
AACGGACTCA CTCCGGGAGG TTTAAGTGGC TCCATCGAGC TCCTGGAGGA TCAGTCCATC 
GGTGCGGATT CAGACTACAG TGCCGCCGGG ATGCTGCGTC TGAGCGAAGG GGTCAACATC 
AGTGCTCTGG ACAGAGGTCA GAGGTCGGAG AGTTCAGAAG GGGCTGTAGG TCACCAAGTC 
ACCAACAACA TTAAAGACGG TTCCAATGGA GGTTTGGGAG AAGGAGGAGG TGACGGCACA 
CAGTTAGAAG ATAGCATATC TCTCATTTCA GGGCAACAGC GTGTGTATGC TCGAATGAGC 
GAGTCGCCTG GGCTGAGAAG ACGGGCAGAT CCTGGCGAAG ACAGGTTAAG CGCAGGGTCG 
TTCCTGTCTT ATCTGCAGGA TCAGGGCTCT CCTGGTTGGC ATTCGGCTGC AGAAGCGACT 
CAGAACAGAT TGGCGTCTTT GCAATGCTCC CTTCCTAGGC AGGCAATAGA GTCTATGATG 
TCATCGGTGC GAGCTACGGT GGACTCTGAC TCCAAGCAGT CTTGTTTGGG CCAGGAGGCT 
CTGATTGAGC CAGTGCGGGA CATGGGGCAC TCTGAGATCA TCCATGGCCA TTATCAGGGG 
ACTCAGCCGT TTGAGAAAGG ACTCGGCTTC CCGCACAGAA CAACCAACAT GCGCACTTGG 
GAGGACATGC GTCTGAGCGG ACAGGGTGAT GAGGCTGAGG ATCTTCCAGG AGAGCAAGTG 
AGCGAGGAGC AATTCACAGA CGAGCATGGC AACATCGTCA CCAAAAAGAT TGTGAGGAAA 
GTGGTCCGGA GAGGAAAAGG GGAGGAAGGG GGTCAGGAGC TGATCATTGA AGGTCTGCAG 
CAGGATATCA GTGAGCCTGA CTTTGATGGA GAACAGTTCA TGAGCTATGC CGTCCTGGGA 
CGTGACAGCA AGCCGGATGT TGTGGATGTG AAGAGAGGAG GTGCTCAGAT AGTCAAATGT 
GCGAGTCTGC GGAGAGTAAA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300282.1
CDS	344..6250
Translation	MPTNTILFGADAATSFLRAARSGNLEKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLINHGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGLTPLHVSSFMGHLNIVKILMQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGANPNAAGKVGLTPLHVAVHHNNLDVVNLLLSKGGSPHSAARNGYTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPLHLASQEGQPDMVLLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKHGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSNGTSALAIAKRLGYISVIDVLKLVTEETVTMTTTEKHRMSFPETVDEILDVSEDEGIAQLTLGEELLGNEGARYLMKMEDIKDHDEDFLSPKKVLEYEGELENHSPAIPRIPCVSPETVMLKEHEMEQHTPLPLPKEYDEDSLIPSSPATETSDNVSPVASPIHTGFLVSFMVDARGGSMRGSRHNGLRVIIPPRTCAAPTRITCRLVKPQKLPTPPPLVEGEGLASRIISLGPASMQFLGPVIVEIPHFAALGRGDRELVVLRSENGSVWKEHRNRYGDEVLETILNGMDEDLESQEELGKKRIRRIISTDFPLYFAVVSRIQQENDLIGPEGGFLSSKLVPMVQATFPETAVTKRVRLGLQAQPVPDELVAKLLGNQATFSPVVTVEPRRRKFHRPIGLRIPLPPSWRESPRDAGEGDTTSLRLLCSVIGGTAPAQWEDITGTTKLAYSNDCASFTTNVSARFWLADCPRTAEAVSFANLLYRELSAVPYMAKFVVFAKMNEAREGRLRCYCMTDDKMDKTLEQHENFSEVARSRDIEVMEGMPLYLECSGNLVPIRKAAQQPRCFSFQAFKDNRLPVSVKVRDSSKDPSGFLSFLRKSTKYEDSQHVLCNLNVTMPPCIKIMGSDERRRTLTPLALRERYSALNEPAMASLSAMERTELKMALIAEQLGLSWAELGRELQFNVDEINKIRVENPNSLLEQSSTLLNLWAAREGKRAKMDSLYVALKSIDRVDIVNMMEGQGGPPAGQQGGWEQDASRSRHHDRDHLSPGITNGYGLVVQEELVSPASMQYSLPSPLGNEPYWQEVSSMECAPIATTEEDTLMEMSEVQVWPSGNSPSIVAVEDSSLECSNADDSEALQNRSLGHSRSGTPGGNGLTPGGLSGSIELLEDQSIGADSDYSAAGMLRLSEGVNISALDRGQRSESSEGAVGHQVTNNIKDGSNGGLGEGGGDGTQLEDSISLISGQQRVYARMSESPGLRRRADPGEDRLSAGSFLSYLQDQGSPGWHSAAEATQNRLASLQCSLPRQAIESMMSSVRATVDSDSKQSCLGQEALIEPVRDMGHSEIIHGHYQGTQPFEKGLGFPHRTTNMRTWEDMRLSGQGDEAEDLPGEQVSEEQFTDEHGNIVTKKIVRKVVRRGKGEEGGQELIIEGLQQDISEPDFDGEQFMSYAVLGRDSKPDVVDVKRGGAQIVKCASLRRVKQ

Target ORF information:

RefSeq Version	XM_009302007.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302007.2

ORF Insert Sequence:

ATGCCAACCA ACACCATCCT TTTTGGAGCT GACGCTGCCA CCAGTTTCCT CCGCGCAGCT 
CGCTCAGGTA ATCTGGAAAA GGCTCTAGAC CACATCAAGA ACGGCATCGA CATCAACACA 
GCCAATCAGA ATGGACTCAA CGGGCTGCAT TTGGCCTCCA AGGAAGGCCA TGTTAAAATG 
GTGCTGGAGC TGCTTCACCA TGGTATTGTA CTTGAGACGA CAACGAAGAA AGGGAACACT 
GCTCTTCATA TAGCGGCGTT GGCTGGCCAG GAGCAGGTGG TCACTGAACT GGTCAACTAT 
GGAGCAAACG TCAACGCTCA GTCTCAGAAG GGCTTCACGC CGCTCTACAT GGCAGCACAA 
GAGAATCATC TGGAGGTTGT GAAGTTTCTG CTGGATAACG GAGCCAATCA GACCATTCCA 
ACTGAGGATG GCTTTACCCC TCTTGCTGTG GCTCTTCAGC AGGGCCATGA GAATGTGGTG 
GCCCTGCTCA TCAACCACGG TACTAAGGGC AAGGTCCGCC TTCCCGCACT GCACATCGCT 
GCCCGAAACG ACGACACCCG CACTGCCGCT GTGCTCTTAC AGAACGACCC CAATCCTGAC 
GTCCTGAGCA AGACAGGCTT CACGCCGCTC CACATCGCAG CACATTATGA AAACCTGAAT 
GTTGCTCAGC TTCTTCTGAA CCGAGGAGCT GATGTGAACT TCACACCTAA GAACGGGATC 
ACACCGCTGC ACATCGCATC CAGGAGAGGG AACGTCATCA TGGTCAGACT TCTGCTGGAC 
CGAGGAGCAA AGATCGATGC CAAGACAAAA GATGAGTTGA CTCCTCTTCA CTGTGCTGCC 
AGAAATGGCC ATGTGAGAAT CATCGAGATC CTTCTGGACC AAGGAGCCCC AATACAGGCC 
AAGACCAAAA ATGGCCTGTC GCCGATCCAC ATGGCAGCCC AGGGCGATCA CTTGGACTGT 
ATCCGACAGC TCTTACAGTA CAACGCAGAG ATCGATGACA TCACTCTGGA CCACCTGACA 
CCTCTGCATG TGGCGGCTCA CTGCGGGCAT CACCGCGTGG CCAAGGTGCT CCTGGATAAA 
GGAGCCAAAC CAAACACCCG CGCTCTGAAT GGATTCACGC CGCTCCACAT TGCCTGTAAG 
AAGAACCACA TGCGTGTAAT GGACCTGCTA TTAAAGCATT CTGCATCCCT GGAAGCTGTA 
ACCGAGTCAG GCTTGACCCC GCTGCATGTG TCCTCCTTCA TGGGTCATCT GAATATAGTC 
AAGATACTGA TGCAAAAAGG AGCTTCGCCG AACGCCTCAA ATGTGAAAGT GGAAACCCCT 
CTGCATATGG CATCTCGAGC TGGACACTGT GAAGTTGCTG AATTCCTATT GCAAAATGCA 
GCTCCAGTTG ATGCCAAAGC CAAGGATGAC CAGACGCCGC TACACTGTGC CTCCCGCATG 
GGCCACAACG AGATGGTGAA GTTGCTTCTG GAGCACAAGG CCAACTCAAA CTCCACCACC 
ACAGCAGGAC ACACACCGCT GCACATCGCG GCCCGCGAGG GACACACACA AACTGCCAGC 
ATCCTCCTGG ACATGAACGC CCAGCTCACC AAAATGACCA AGAAAGGCTT CACTCCTCTT 
CATGTTGCAG CCAAATACGG AAAGGTGGAC GTGGCAGTTC TGCTTCTGGA GAGGGGAGCA 
AATCCAAACG CTGCTGGAAA AGTTGGACTC ACGCCGCTGC ATGTGGCCGT CCATCATAAC 
AATCTAGATG TGGTCAATCT TTTACTCAGC AAAGGAGGAT CACCACACAG CGCCGCTCGG 
AATGGCTATA CTCCGCTCCA CATTGCTTCC AAACAGAACC AGGCGGAGGT GGCGAGCAGT 
TTACTGCAGC ACGGGGCGTC TGCCAATGCT GAGTCTCTGC AAGGGGTCAC ACCTCTACAC 
CTCGCCTCTC AAGAGGGTCA ACCTGACATG GTGTTACTGC TCATCTCCAA GCAGGCCAAT 
GTAAACCTTG GCAATAAGAG CGGTCTGACT CCTCTTCATC TTGTGGCTCA GGAAGGGCAT 
GTTGGGATTG CTGATATACT AGTGAAACAT GGAGCATCTG TTTATGCTGC CACACGGATG 
GGTTACACTC CGCTTCACGT GGCTTGTCAT TATGGGAATA TCAAGATGGT GAAGTTTCTC 
TTACAGCAGC AGGCCAACGT AAACTGCAAG ACCAGGATGA GCTACACGCC GCTACATCAG 
GCTGCTCAGC AGGGTCATAC GGACATCGTG ACGCTTCTGC TGAAACACGG AGCACAACCG 
AATGAAGTTA CATCTAACGG CACCTCTGCT CTTGCCATCG CCAAGCGGCT TGGATACATT 
TCAGTCATCG ATGTGCTCAA ACTGGTCACT GAAGAGACGG TCACTATGAC CACCACTGAA 
AAACATCGCA TGAGTTTCCC TGAAACCGTT GATGAGATCC TGGATGTGTC TGAAGATGAA 
GGAATTGCAC AGCTAACATT AGGCGAGGAG CTGCTGGGAA ACGAGGGCGC CAGGTATTTG 
ATGAAGATGG AGGACATAAA GGACCATGAT GAGGATTTCC TTTCCCCCAA GAAAGTTCTA 
GAGTACGAGG GAGAGTTGGA GAATCATTCT CCTGCTATCC CGAGGATCCC GTGTGTGTCT 
CCTGAGACTG TCATGCTGAA AGAGCATGAG ATGGAGCAGC ACACTCCACT TCCATTGCCC 
AAAGAGTACG ACGAGGATTC TCTGATTCCC AGCAGTCCTG CAACTGAGAC TTCAGACAAT 
GTCAGTCCGG TTGCCAGTCC CATCCACACC GGTTTTCTGG TGAGCTTCAT GGTGGATGCT 
CGCGGCGGCT CGATGCGCGG CAGCAGACAC AACGGCCTCC GCGTCATCAT TCCTCCTCGA 
ACCTGCGCCG CTCCCACACG CATCACCTGC CGCCTGGTGA AGCCCCAGAA GCTGCCCACA 
CCGCCGCCAT TAGTGGAGGG AGAGGGTCTC GCCAGCCGCA TCATCTCCCT TGGACCCGCC 
AGCATGCAGT TTTTAGGGCC TGTGATTGTG GAGATTCCTC ATTTTGCGGC TCTCGGTCGG 
GGAGATCGGG AACTCGTGGT CTTGAGGAGT GAGAACGGAT CGGTCTGGAA GGAACATCGT 
AACCGATATG GAGATGAAGT GCTAGAGACC ATCCTCAACG GCATGGATGA AGATCTGGAG 
AGTCAGGAGG AGCTCGGGAA GAAACGCATC AGACGGATCA TCTCCACCGA CTTCCCGCTC 
TACTTTGCGG TGGTGTCTCG CATCCAGCAG GAGAACGATC TGATTGGCCC AGAAGGAGGT 
TTTCTGTCCA GTAAACTGGT GCCGATGGTC CAGGCCACCT TTCCAGAGAC AGCCGTCACC 
AAACGCGTGC GTCTGGGACT GCAGGCCCAG CCGGTGCCTG ATGAGCTGGT GGCCAAGCTT 
TTGGGGAACC AGGCCACGTT CAGTCCTGTG GTGACGGTGG AGCCTCGGCG CCGGAAGTTT 
CACCGTCCCA TCGGCCTAAG AATCCCACTG CCACCCTCAT GGAGAGAGAG CCCGCGGGAC 
GCCGGAGAGG GAGACACCAC CAGCCTGCGC CTGCTCTGCA GCGTCATCGG TGGAACAGCT 
CCTGCCCAGT GGGAAGATAT AACTGGCACC ACCAAACTGG CGTATTCCAA TGACTGTGCC 
AGTTTCACCA CCAACGTCTC TGCCCGATTC TGGCTGGCGG ACTGTCCTCG GACTGCGGAG 
GCCGTGTCCT TTGCCAACCT CCTGTACCGC GAGCTGTCGG CCGTGCCGTA CATGGCCAAG 
TTTGTAGTGT TTGCGAAGAT GAATGAAGCT CGCGAGGGTC GACTCCGCTG CTACTGCATG 
ACGGATGACA AGATGGACAA AACCCTGGAG CAGCACGAGA ACTTCTCGGA AGTGGCTCGC 
AGCAGAGACA TCGAGGTGAT GGAGGGAATG CCGCTGTATT TGGAGTGTTC AGGAAACTTG 
GTTCCTATAA GAAAAGCAGC CCAGCAGCCT CGCTGTTTCA GCTTCCAGGC CTTTAAAGAC 
AACAGACTGC CTGTGTCCGT CAAGGTGAGG GACAGCAGTA AAGACCCCTC TGGGTTTTTG 
TCTTTCTTGC GCAAGTCAAC CAAATATGAA GACAGCCAAC ACGTGCTGTG CAACCTCAAC 
GTGACCATGC CGCCGTGCAT TAAGATTATG GGCAGCGATG AGCGGAGGAG GACTCTGACT 
CCTCTGGCTC TCAGAGAAAG ATACAGCGCT CTGAATGAAC CGGCAATGGC ATCACTGAGC 
GCCATGGAAA GGACGGAGTT AAAGATGGCT TTAATAGCTG AACAGCTAGG CCTGAGCTGG 
GCTGAGTTGG GACGGGAACT GCAGTTTAAT GTAGATGAAA TAAATAAGAT CCGTGTGGAG 
AATCCCAACT CCCTTTTAGA GCAGAGCTCA ACGCTGCTCA ACCTGTGGGC CGCCCGAGAG 
GGGAAGAGAG CCAAAATGGA CAGCCTGTAC GTGGCCCTCA AGAGCATCGA CCGAGTGGAC 
ATTGTGAACA TGATGGAGGG CCAGGGAGGA CCACCGGCGG GTCAGCAGGG GGGCTGGGAG 
CAGGACGCTT CTAGGTCTAG GCATCACGAC CGAGACCATC TCTCACCTGG AATCACAAAT 
GGTTACGGGC TGGTGGTGCA GGAGGAACTA GTGTCACCGG CCTCCATGCA GTACAGTCTG 
CCCTCGCCAC TGGGCAATGA GCCCTACTGG CAGGAGGTTT CCAGCATGGA GTGCGCGCCC 
ATCGCCACCA CTGAAGAAGA CACGCTCATG GAGATGTCCG AGGTACAGGT TTGGCCCTCA 
GGAAATAGTC CGTCCATTGT GGCTGTAGAG GACTCCTCAT TGGAGTGCAG CAATGCGGAC 
GACTCCGAGG CGCTGCAGAA CCGGAGCCTG GGTCACTCCA GGAGCGGGAC GCCCGGTGGG 
AACGGACTCA CTCCGGGAGG TTTAAGTGGC TCCATCGAGC TCCTGGAGGA TCAGTCCATC 
GGTGCGGATT CAGACTACAG TGCCGCCGGG ATGCTGCGTC TGAGCGAAGG GGTCAACATC 
AGTGCTCTGG ACAGAGGTCA GAGGTCGGAG AGTTCAGAAG GGGCTGTAGG TCACCAAGTC 
ACCAACAACA TTAAAGACGG TTCCAATGGA GGTTTGGGAG AAGGAGGAGG TGACGGCACA 
CAGTTAGAAG ATAGCATATC TCTCATTTCA GGGCAACAGC GTGTGTATGC TCGAATGAGC 
GAGTCGCCTG GGCTGAGAAG ACGGGCAGAT CCTGGCGAAG ACAGGTTAAG CGCAGGGTCG 
TTCCTGTCTT ATCTGCAGGA TCAGGGCTCT CCTGGTTGGC ATTCGGCTGC AGAAGCGACT 
CAGAACAGAT TGGCGTCTTT GCAATGCTCC CTTCCTAGGC AGGCAATAGA GTCTATGATG 
TCATCGGTGC GAGCTACGGT GGACTCTGAC TCCAAGCAGT CTTGTTTGGG CCAGGAGGCT 
CTGATTGAGC CAGTGCGGGA CATGGGGCAC TCTGAGATCA TCCATGGCCA TTATCAGGGG 
ACTCAGCCGT TTGAGAAAGG ACTCGGCTTC CCGCACAGAA CAACCAACAT GCGCACTTGG 
GAGGACATGC GTCTGAGCGG ACAGGGTGAT GAGGCTGAGG ATCTTCCAGG AGAGCAAGTG 
AGCGAGGAGC AATTCACAGA CGAGCATGGC AACATCGTCA CCAAAAAGAT TGTGAGGAAA 
GTGGTCCGGA GAGGAAAAGG GGAGGAAGGG GGTCAGGAGC TGATCATTGA AGGTCTGCAG 
CAGGATATCA GTGAGCCTGA CTTTGATGGA GAACAGTTCA TGAGCTATGC CGTCCTGGGA 
CGTGACAGCA AGCCGGATGT TGTGGATGTG AAGAGAGGAG GTGCTCAGAT AGTCAAATGT 
GCGAGTCTGC GGAGAGTAAA GCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_009302008.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009300283.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X2, mRNA.

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CloneID	ODa41280
Clone ID Related Accession (Same CDS sequence)	XM_009302008.2
Accession Version	XM_009302008.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5892bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ankyrin 1, erythrocytic a isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302008.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAACCA ACACCATCCT TTTTGGAGCT GACGCTGCCA CCAGTTTCCT CCGCGCAGCT 
CGCTCAGGTA ATCTGGAAAA GGCTCTAGAC CACATCAAGA ACGGCATCGA CATCAACACA 
GCCAATCAGA ATGGACTCAA CGGGCTGCAT TTGGCCTCCA AGGAAGGCCA TGTTAAAATG 
GTGCTGGAGC TGCTTCACCA TGGTATTGTA CTTGAGACGA CAACGAAGAA AGGGAACACT 
GCTCTTCATA TAGCGGCGTT GGCTGGCCAG GAGCAGGTGG TCACTGAACT GGTCAACTAT 
GGAGCAAACG TCAACGCTCA GTCTCAGAAG GGCTTCACGC CGCTCTACAT GGCAGCACAA 
GAGAATCATC TGGAGGTTGT GAAGTTTCTG CTGGATAACG GAGCCAATCA GACCATTCCA 
ACTGAGGATG GCTTTACCCC TCTTGCTGTG GCTCTTCAGC AGGGCCATGA GAATGTGGTG 
GCCCTGCTCA TCAACCACGG TACTAAGGGC AAGGTCCGCC TTCCCGCACT GCACATCGCT 
GCCCGAAACG ACGACACCCG CACTGCCGCT GTGCTCTTAC AGAACGACCC CAATCCTGAC 
GTCCTGAGCA AGACAGGCTT CACGCCGCTC CACATCGCAG CACATTATGA AAACCTGAAT 
GTTGCTCAGC TTCTTCTGAA CCGAGGAGCT GATGTGAACT TCACACCTAA GAACGGGATC 
ACACCGCTGC ACATCGCATC CAGGAGAGGG AACGTCATCA TGGTCAGACT TCTGCTGGAC 
CGAGGAGCAA AGATCGATGC CAAGACAAAA GATGAGTTGA CTCCTCTTCA CTGTGCTGCC 
AGAAATGGCC ATGTGAGAAT CATCGAGATC CTTCTGGACC AAGGAGCCCC AATACAGGCC 
AAGACCAAAA ATGGCCTGTC GCCGATCCAC ATGGCAGCCC AGGGCGATCA CTTGGACTGT 
ATCCGACAGC TCTTACAGTA CAACGCAGAG ATCGATGACA TCACTCTGGA CCACCTGACA 
CCTCTGCATG TGGCGGCTCA CTGCGGGCAT CACCGCGTGG CCAAGGTGCT CCTGGATAAA 
GGAGCCAAAC CAAACACCCG CGCTCTGAAT GGATTCACGC CGCTCCACAT TGCCTGTAAG 
AAGAACCACA TGCGTGTAAT GGACCTGCTA TTAAAGCATT CTGCATCCCT GGAAGCTGTA 
ACCGAGTCAG GCTTGACCCC GCTGCATGTG TCCTCCTTCA TGGGTCATCT GAATATAGTC 
AAGATACTGA TGCAAAAAGG AGCTTCGCCG AACGCCTCAA ATGTGAAAGT GGAAACCCCT 
CTGCATATGG CATCTCGAGC TGGACACTGT GAAGTTGCTG AATTCCTATT GCAAAATGCA 
GCTCCAGTTG ATGCCAAAGC CAAGGATGAC CAGACGCCGC TACACTGTGC CTCCCGCATG 
GGCCACAACG AGATGGTGAA GTTGCTTCTG GAGCACAAGG CCAACTCAAA CTCCACCACC 
ACAGCAGGAC ACACACCGCT GCACATCGCG GCCCGCGAGG GACACACACA AACTGCCAGC 
ATCCTCCTGG ACATGAACGC CCAGCTCACC AAAATGACCA AGAAAGGCTT CACTCCTCTT 
CATGTTGCAG CCAAATACGG AAAGGTGGAC GTGGCAGTTC TGCTTCTGGA GAGGGGAGCA 
AATCCAAACG CTGCTGGAAA AGTTGGACTC ACGCCGCTGC ATGTGGCCGT CCATCATAAC 
AATCTAGATG TGGTCAATCT TTTACTCAGC AAAGGAGGAT CACCACACAG CGCCGCTCGG 
AATGGCTATA CTCCGCTCCA CATTGCTTCC AAACAGAACC AGGCGGAGGT GGCGAGCAGT 
TTACTGCAGC ACGGGGCGTC TGCCAATGCT GAGTCTCTGC AAGGGGTCAC ACCTCTACAC 
CTCGCCTCTC AAGAGGGTCA ACCTGACATG GTGTTACTGC TCATCTCCAA GCAGGCCAAT 
GTAAACCTTG GCAATAAGAG CGGTCTGACT CCTCTTCATC TTGTGGCTCA GGAAGGGCAT 
GTTGGGATTG CTGATATACT AGTGAAACAT GGAGCATCTG TTTATGCTGC CACACGGATG 
GGTTACACTC CGCTTCACGT GGCTTGTCAT TATGGGAATA TCAAGATGGT GAAGTTTCTC 
TTACAGCAGC AGGCCAACGT AAACTGCAAG ACCAGGATGA GCTACACGCC GCTACATCAG 
GCTGCTCAGC AGGGTCATAC GGACATCGTG ACGCTTCTGC TGAAACACGG AGCACAACCG 
AATGAAGTTA CATCTAACGG CACCTCTGCT CTTGCCATCG CCAAGCGGCT TGGATACATT 
TCAGTCATCG ATGTGCTCAA ACTGGTCACT GAAGAGACGG TCACTATGAC CACCACTGAA 
AAACATCGCA TGAGTTTCCC TGAAACCGTT GATGAGATCC TGGATGTGTC TGAAGATGAA 
GGAATTGCAC AGCTAACATT AGGCGAGGAG CTGCTGGGAA ACGAGGGCGC CAGGTATTTG 
ATGAAGATGG AGGACATAAA GGACCATGAT GAGGATTTCC TTTCCCCCAA GAAAGTTCTA 
GAGAATCATT CTCCTGCTAT CCCGAGGATC CCGTGTGTGT CTCCTGAGAC TGTCATGCTG 
AAAGAGCATG AGATGGAGCA GCAGCACACT CCACTTCCAT TGCCCAAAGA GTACGACGAG 
GATTCTCTGA TTCCCAGCAG TCCTGCAACT GAGACTTCAG ACAATGTCAG TCCGGTTGCC 
AGTCCCATCC ACACCGGTTT TCTGGTGAGC TTCATGGTGG ATGCTCGCGG CGGCTCGATG 
CGCGGCAGCA GACACAACGG CCTCCGCGTC ATCATTCCTC CTCGAACCTG CGCCGCTCCC 
ACACGCATCA CCTGCCGCCT GGTGAAGCCC CAGAAGCTGC CCACACCGCC GCCATTAGTG 
GAGGGAGAGG GTCTCGCCAG CCGCATCATC TCCCTTGGAC CCGCCAGCAT GCAGTTTTTA 
GGGCCTGTGA TTGTGGAGAT TCCTCATTTT GCGGCTCTCG GTCGGGGAGA TCGGGAACTC 
GTGGTCTTGA GGAGTGAGAA CGGATCGGTC TGGAAGGAAC ATCGTAACCG ATATGGAGAT 
GAAGTGCTAG AGACCATCCT CAACGGCATG GATGAAGATC TGGAGAGTCA GGAGGAGCTC 
GGGAAGAAAC GCATCAGACG GATCATCTCC ACCGACTTCC CGCTCTACTT TGCGGTGGTG 
TCTCGCATCC AGCAGGAGAA CGATCTGATT GGCCCAGAAG GAGGTTTTCT GTCCAGTAAA 
CTGGTGCCGA TGGTCCAGGC CACCTTTCCA GAGACAGCCG TCACCAAACG CGTGCGTCTG 
GGACTGCAGG CCCAGCCGGT GCCTGATGAG CTGGTGGCCA AGCTTTTGGG GAACCAGGCC 
ACGTTCAGTC CTGTGGTGAC GGTGGAGCCT CGGCGCCGGA AGTTTCACCG TCCCATCGGC 
CTAAGAATCC CACTGCCACC CTCATGGAGA GAGAGCCCGC GGGACGCCGG AGAGGGAGAC 
ACCACCAGCC TGCGCCTGCT CTGCAGCGTC ATCGGTGGAA CAGCTCCTGC CCAGTGGGAA 
GATATAACTG GCACCACCAA ACTGGCGTAT TCCAATGACT GTGCCAGTTT CACCACCAAC 
GTCTCTGCCC GATTCTGGCT GGCGGACTGT CCTCGGACTG CGGAGGCCGT GTCCTTTGCC 
AACCTCCTGT ACCGCGAGCT GTCGGCCGTG CCGTACATGG CCAAGTTTGT AGTGTTTGCG 
AAGATGAATG AAGCTCGCGA GGGTCGACTC CGCTGCTACT GCATGACGGA TGACAAGATG 
GACAAAACCC TGGAGCAGCA CGAGAACTTC TCGGAAGTGG CTCGCAGCAG AGACATCGAG 
GTGATGGAGG GAATGCCGCT GTATTTGGAG TGTTCAGGAA ACTTGGTTCC TATAAGAAAA 
GCAGCCCAGC AGCCTCGCTG TTTCAGCTTC CAGGCCTTTA AAGACAACAG ACTGCCTGTG 
TCCGTCAAGG TGAGGGACAG CAGTAAAGAC CCCTCTGGGT TTTTGTCTTT CTTGCGCAAG 
TCAACCAAAT ATGAAGACAG CCAACACGTG CTGTGCAACC TCAACGTGAC CATGCCGCCG 
TGCATTAAGA TTATGGGCAG CGATGAGCGG AGGAGGACTC TGACTCCTCT GGCTCTCAGA 
GAAAGATACA GCGCTCTGAA TGAACCGGCA ATGGCATCAC TGAGCGCCAT GGAAAGGACG 
GAGTTAAAGA TGGCTTTAAT AGCTGAACAG CTAGGCCTGA GCTGGGCTGA GTTGGGACGG 
GAACTGCAGT TTAATGTAGA TGAAATAAAT AAGATCCGTG TGGAGAATCC CAACTCCCTT 
TTAGAGCAGA GCTCAACGCT GCTCAACCTG TGGGCCGCCC GAGAGGGGAA GAGAGCCAAA 
ATGGACAGCC TGTACGTGGC CCTCAAGAGC ATCGACCGAG TGGACATTGT GAACATGATG 
GAGGGCCAGG GAGGACCACC GGCGGGTCAG CAGGGGGGCT GGGAGCAGGA CGCTTCTAGG 
TCTAGGCATC ACGACCGAGA CCATCTCTCA CCTGGAATCA CAAATGGTTA CGGGCTGGTG 
GTGCAGGAGG AACTAGTGTC ACCGGCCTCC ATGCAGTACA GTCTGCCCTC GCCACTGGGC 
AATGAGCCCT ACTGGCAGGA GGTTTCCAGC ATGGAGTGCG CGCCCATCGC CACCACTGAA 
GAAGACACGC TCATGGAGAT GTCCGAGGTA CAGGTTTGGC CCTCAGGAAA TAGTCCGTCC 
ATTGTGGCTG TAGAGGACTC CTCATTGGAG TGCAGCAATG CGGACGACTC CGAGGCGCTG 
CAGAACCGGA GCCTGGGTCA CTCCAGGAGC GGGACGCCCG GTGGGAACGG ACTCACTCCG 
GGAGGTTTAA GTGGCTCCAT CGAGCTCCTG GAGGATCAGT CCATCGGTGC GGATTCAGAC 
TACAGTGCCG CCGGGATGCT GCGTCTGAGC GAAGGGGTCA ACATCAGTGC TCTGGACAGA 
GGTCAGAGGT CGGAGAGTTC AGAAGGGGCT GTAGGTCACC AAGTCACCAA CAACATTAAA 
GACGGTTCCA ATGGAGGTTT GGGAGAAGGA GGAGGTGACG GCACACAGTT AGAAGATAGC 
ATATCTCTCA TTTCAGGGCA ACAGCGTGTG TATGCTCGAA TGAGCGAGTC GCCTGGGCTG 
AGAAGACGGG CAGATCCTGG CGAAGACAGG TTAAGCGCAG GGTCGTTCCT GTCTTATCTG 
CAGGATCAGG GCTCTCCTGG TTGGCATTCG GCTGCAGAAG CGACTCAGAA CAGATTGGCG 
TCTTTGCAAT GCTCCCTTCC TAGGCAGGCA ATAGAGTCTA TGATGTCATC GGTGCGAGCT 
ACGGTGGACT CTGACTCCAA GCAGTCTTGT TTGGGCCAGG AGGCTCTGAT TGAGCCAGTG 
CGGGACATGG GGCACTCTGA GATCATCCAT GGCCATTATC AGGGGACTCA GCCGTTTGAG 
AAAGGACTCG GCTTCCCGCA CAGAACAACC AACATGCGCA CTTGGGAGGA CATGCGTCTG 
AGCGGACAGG GTGATGAGGC TGAGGATCTT CCAGGAGAGC AAGTGAGCGA GGAGCAATTC 
ACAGACGAGC ATGGCAACAT CGTCACCAAA AAGATTGTGA GGAAAGTGGT CCGGAGAGGA 
AAAGGGGAGG AAGGGGGTCA GGAGCTGATC ATTGAAGGTC TGCAGCAGGA TATCAGTGAG 
CCTGACTTTG ATGGAGAACA GTTCATGAGC TATGCCGTCC TGGGACGTGA CAGCAAGCCG 
GATGTTGTGG ATGTGAAGAG AGGAGGTGCT CAGATAGTCA AATGTGCGAG TCTGCGGAGA 
GTAAAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300283.1
CDS	342..6233
Translation	MPTNTILFGADAATSFLRAARSGNLEKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLINHGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGLTPLHVSSFMGHLNIVKILMQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGANPNAAGKVGLTPLHVAVHHNNLDVVNLLLSKGGSPHSAARNGYTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPLHLASQEGQPDMVLLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKHGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSNGTSALAIAKRLGYISVIDVLKLVTEETVTMTTTEKHRMSFPETVDEILDVSEDEGIAQLTLGEELLGNEGARYLMKMEDIKDHDEDFLSPKKVLENHSPAIPRIPCVSPETVMLKEHEMEQQHTPLPLPKEYDEDSLIPSSPATETSDNVSPVASPIHTGFLVSFMVDARGGSMRGSRHNGLRVIIPPRTCAAPTRITCRLVKPQKLPTPPPLVEGEGLASRIISLGPASMQFLGPVIVEIPHFAALGRGDRELVVLRSENGSVWKEHRNRYGDEVLETILNGMDEDLESQEELGKKRIRRIISTDFPLYFAVVSRIQQENDLIGPEGGFLSSKLVPMVQATFPETAVTKRVRLGLQAQPVPDELVAKLLGNQATFSPVVTVEPRRRKFHRPIGLRIPLPPSWRESPRDAGEGDTTSLRLLCSVIGGTAPAQWEDITGTTKLAYSNDCASFTTNVSARFWLADCPRTAEAVSFANLLYRELSAVPYMAKFVVFAKMNEAREGRLRCYCMTDDKMDKTLEQHENFSEVARSRDIEVMEGMPLYLECSGNLVPIRKAAQQPRCFSFQAFKDNRLPVSVKVRDSSKDPSGFLSFLRKSTKYEDSQHVLCNLNVTMPPCIKIMGSDERRRTLTPLALRERYSALNEPAMASLSAMERTELKMALIAEQLGLSWAELGRELQFNVDEINKIRVENPNSLLEQSSTLLNLWAAREGKRAKMDSLYVALKSIDRVDIVNMMEGQGGPPAGQQGGWEQDASRSRHHDRDHLSPGITNGYGLVVQEELVSPASMQYSLPSPLGNEPYWQEVSSMECAPIATTEEDTLMEMSEVQVWPSGNSPSIVAVEDSSLECSNADDSEALQNRSLGHSRSGTPGGNGLTPGGLSGSIELLEDQSIGADSDYSAAGMLRLSEGVNISALDRGQRSESSEGAVGHQVTNNIKDGSNGGLGEGGGDGTQLEDSISLISGQQRVYARMSESPGLRRRADPGEDRLSAGSFLSYLQDQGSPGWHSAAEATQNRLASLQCSLPRQAIESMMSSVRATVDSDSKQSCLGQEALIEPVRDMGHSEIIHGHYQGTQPFEKGLGFPHRTTNMRTWEDMRLSGQGDEAEDLPGEQVSEEQFTDEHGNIVTKKIVRKVVRRGKGEEGGQELIIEGLQQDISEPDFDGEQFMSYAVLGRDSKPDVVDVKRGGAQIVKCASLRRVKQ

Target ORF information:

RefSeq Version	XM_009302008.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302008.2

ORF Insert Sequence:

ATGCCAACCA ACACCATCCT TTTTGGAGCT GACGCTGCCA CCAGTTTCCT CCGCGCAGCT 
CGCTCAGGTA ATCTGGAAAA GGCTCTAGAC CACATCAAGA ACGGCATCGA CATCAACACA 
GCCAATCAGA ATGGACTCAA CGGGCTGCAT TTGGCCTCCA AGGAAGGCCA TGTTAAAATG 
GTGCTGGAGC TGCTTCACCA TGGTATTGTA CTTGAGACGA CAACGAAGAA AGGGAACACT 
GCTCTTCATA TAGCGGCGTT GGCTGGCCAG GAGCAGGTGG TCACTGAACT GGTCAACTAT 
GGAGCAAACG TCAACGCTCA GTCTCAGAAG GGCTTCACGC CGCTCTACAT GGCAGCACAA 
GAGAATCATC TGGAGGTTGT GAAGTTTCTG CTGGATAACG GAGCCAATCA GACCATTCCA 
ACTGAGGATG GCTTTACCCC TCTTGCTGTG GCTCTTCAGC AGGGCCATGA GAATGTGGTG 
GCCCTGCTCA TCAACCACGG TACTAAGGGC AAGGTCCGCC TTCCCGCACT GCACATCGCT 
GCCCGAAACG ACGACACCCG CACTGCCGCT GTGCTCTTAC AGAACGACCC CAATCCTGAC 
GTCCTGAGCA AGACAGGCTT CACGCCGCTC CACATCGCAG CACATTATGA AAACCTGAAT 
GTTGCTCAGC TTCTTCTGAA CCGAGGAGCT GATGTGAACT TCACACCTAA GAACGGGATC 
ACACCGCTGC ACATCGCATC CAGGAGAGGG AACGTCATCA TGGTCAGACT TCTGCTGGAC 
CGAGGAGCAA AGATCGATGC CAAGACAAAA GATGAGTTGA CTCCTCTTCA CTGTGCTGCC 
AGAAATGGCC ATGTGAGAAT CATCGAGATC CTTCTGGACC AAGGAGCCCC AATACAGGCC 
AAGACCAAAA ATGGCCTGTC GCCGATCCAC ATGGCAGCCC AGGGCGATCA CTTGGACTGT 
ATCCGACAGC TCTTACAGTA CAACGCAGAG ATCGATGACA TCACTCTGGA CCACCTGACA 
CCTCTGCATG TGGCGGCTCA CTGCGGGCAT CACCGCGTGG CCAAGGTGCT CCTGGATAAA 
GGAGCCAAAC CAAACACCCG CGCTCTGAAT GGATTCACGC CGCTCCACAT TGCCTGTAAG 
AAGAACCACA TGCGTGTAAT GGACCTGCTA TTAAAGCATT CTGCATCCCT GGAAGCTGTA 
ACCGAGTCAG GCTTGACCCC GCTGCATGTG TCCTCCTTCA TGGGTCATCT GAATATAGTC 
AAGATACTGA TGCAAAAAGG AGCTTCGCCG AACGCCTCAA ATGTGAAAGT GGAAACCCCT 
CTGCATATGG CATCTCGAGC TGGACACTGT GAAGTTGCTG AATTCCTATT GCAAAATGCA 
GCTCCAGTTG ATGCCAAAGC CAAGGATGAC CAGACGCCGC TACACTGTGC CTCCCGCATG 
GGCCACAACG AGATGGTGAA GTTGCTTCTG GAGCACAAGG CCAACTCAAA CTCCACCACC 
ACAGCAGGAC ACACACCGCT GCACATCGCG GCCCGCGAGG GACACACACA AACTGCCAGC 
ATCCTCCTGG ACATGAACGC CCAGCTCACC AAAATGACCA AGAAAGGCTT CACTCCTCTT 
CATGTTGCAG CCAAATACGG AAAGGTGGAC GTGGCAGTTC TGCTTCTGGA GAGGGGAGCA 
AATCCAAACG CTGCTGGAAA AGTTGGACTC ACGCCGCTGC ATGTGGCCGT CCATCATAAC 
AATCTAGATG TGGTCAATCT TTTACTCAGC AAAGGAGGAT CACCACACAG CGCCGCTCGG 
AATGGCTATA CTCCGCTCCA CATTGCTTCC AAACAGAACC AGGCGGAGGT GGCGAGCAGT 
TTACTGCAGC ACGGGGCGTC TGCCAATGCT GAGTCTCTGC AAGGGGTCAC ACCTCTACAC 
CTCGCCTCTC AAGAGGGTCA ACCTGACATG GTGTTACTGC TCATCTCCAA GCAGGCCAAT 
GTAAACCTTG GCAATAAGAG CGGTCTGACT CCTCTTCATC TTGTGGCTCA GGAAGGGCAT 
GTTGGGATTG CTGATATACT AGTGAAACAT GGAGCATCTG TTTATGCTGC CACACGGATG 
GGTTACACTC CGCTTCACGT GGCTTGTCAT TATGGGAATA TCAAGATGGT GAAGTTTCTC 
TTACAGCAGC AGGCCAACGT AAACTGCAAG ACCAGGATGA GCTACACGCC GCTACATCAG 
GCTGCTCAGC AGGGTCATAC GGACATCGTG ACGCTTCTGC TGAAACACGG AGCACAACCG 
AATGAAGTTA CATCTAACGG CACCTCTGCT CTTGCCATCG CCAAGCGGCT TGGATACATT 
TCAGTCATCG ATGTGCTCAA ACTGGTCACT GAAGAGACGG TCACTATGAC CACCACTGAA 
AAACATCGCA TGAGTTTCCC TGAAACCGTT GATGAGATCC TGGATGTGTC TGAAGATGAA 
GGAATTGCAC AGCTAACATT AGGCGAGGAG CTGCTGGGAA ACGAGGGCGC CAGGTATTTG 
ATGAAGATGG AGGACATAAA GGACCATGAT GAGGATTTCC TTTCCCCCAA GAAAGTTCTA 
GAGAATCATT CTCCTGCTAT CCCGAGGATC CCGTGTGTGT CTCCTGAGAC TGTCATGCTG 
AAAGAGCATG AGATGGAGCA GCAGCACACT CCACTTCCAT TGCCCAAAGA GTACGACGAG 
GATTCTCTGA TTCCCAGCAG TCCTGCAACT GAGACTTCAG ACAATGTCAG TCCGGTTGCC 
AGTCCCATCC ACACCGGTTT TCTGGTGAGC TTCATGGTGG ATGCTCGCGG CGGCTCGATG 
CGCGGCAGCA GACACAACGG CCTCCGCGTC ATCATTCCTC CTCGAACCTG CGCCGCTCCC 
ACACGCATCA CCTGCCGCCT GGTGAAGCCC CAGAAGCTGC CCACACCGCC GCCATTAGTG 
GAGGGAGAGG GTCTCGCCAG CCGCATCATC TCCCTTGGAC CCGCCAGCAT GCAGTTTTTA 
GGGCCTGTGA TTGTGGAGAT TCCTCATTTT GCGGCTCTCG GTCGGGGAGA TCGGGAACTC 
GTGGTCTTGA GGAGTGAGAA CGGATCGGTC TGGAAGGAAC ATCGTAACCG ATATGGAGAT 
GAAGTGCTAG AGACCATCCT CAACGGCATG GATGAAGATC TGGAGAGTCA GGAGGAGCTC 
GGGAAGAAAC GCATCAGACG GATCATCTCC ACCGACTTCC CGCTCTACTT TGCGGTGGTG 
TCTCGCATCC AGCAGGAGAA CGATCTGATT GGCCCAGAAG GAGGTTTTCT GTCCAGTAAA 
CTGGTGCCGA TGGTCCAGGC CACCTTTCCA GAGACAGCCG TCACCAAACG CGTGCGTCTG 
GGACTGCAGG CCCAGCCGGT GCCTGATGAG CTGGTGGCCA AGCTTTTGGG GAACCAGGCC 
ACGTTCAGTC CTGTGGTGAC GGTGGAGCCT CGGCGCCGGA AGTTTCACCG TCCCATCGGC 
CTAAGAATCC CACTGCCACC CTCATGGAGA GAGAGCCCGC GGGACGCCGG AGAGGGAGAC 
ACCACCAGCC TGCGCCTGCT CTGCAGCGTC ATCGGTGGAA CAGCTCCTGC CCAGTGGGAA 
GATATAACTG GCACCACCAA ACTGGCGTAT TCCAATGACT GTGCCAGTTT CACCACCAAC 
GTCTCTGCCC GATTCTGGCT GGCGGACTGT CCTCGGACTG CGGAGGCCGT GTCCTTTGCC 
AACCTCCTGT ACCGCGAGCT GTCGGCCGTG CCGTACATGG CCAAGTTTGT AGTGTTTGCG 
AAGATGAATG AAGCTCGCGA GGGTCGACTC CGCTGCTACT GCATGACGGA TGACAAGATG 
GACAAAACCC TGGAGCAGCA CGAGAACTTC TCGGAAGTGG CTCGCAGCAG AGACATCGAG 
GTGATGGAGG GAATGCCGCT GTATTTGGAG TGTTCAGGAA ACTTGGTTCC TATAAGAAAA 
GCAGCCCAGC AGCCTCGCTG TTTCAGCTTC CAGGCCTTTA AAGACAACAG ACTGCCTGTG 
TCCGTCAAGG TGAGGGACAG CAGTAAAGAC CCCTCTGGGT TTTTGTCTTT CTTGCGCAAG 
TCAACCAAAT ATGAAGACAG CCAACACGTG CTGTGCAACC TCAACGTGAC CATGCCGCCG 
TGCATTAAGA TTATGGGCAG CGATGAGCGG AGGAGGACTC TGACTCCTCT GGCTCTCAGA 
GAAAGATACA GCGCTCTGAA TGAACCGGCA ATGGCATCAC TGAGCGCCAT GGAAAGGACG 
GAGTTAAAGA TGGCTTTAAT AGCTGAACAG CTAGGCCTGA GCTGGGCTGA GTTGGGACGG 
GAACTGCAGT TTAATGTAGA TGAAATAAAT AAGATCCGTG TGGAGAATCC CAACTCCCTT 
TTAGAGCAGA GCTCAACGCT GCTCAACCTG TGGGCCGCCC GAGAGGGGAA GAGAGCCAAA 
ATGGACAGCC TGTACGTGGC CCTCAAGAGC ATCGACCGAG TGGACATTGT GAACATGATG 
GAGGGCCAGG GAGGACCACC GGCGGGTCAG CAGGGGGGCT GGGAGCAGGA CGCTTCTAGG 
TCTAGGCATC ACGACCGAGA CCATCTCTCA CCTGGAATCA CAAATGGTTA CGGGCTGGTG 
GTGCAGGAGG AACTAGTGTC ACCGGCCTCC ATGCAGTACA GTCTGCCCTC GCCACTGGGC 
AATGAGCCCT ACTGGCAGGA GGTTTCCAGC ATGGAGTGCG CGCCCATCGC CACCACTGAA 
GAAGACACGC TCATGGAGAT GTCCGAGGTA CAGGTTTGGC CCTCAGGAAA TAGTCCGTCC 
ATTGTGGCTG TAGAGGACTC CTCATTGGAG TGCAGCAATG CGGACGACTC CGAGGCGCTG 
CAGAACCGGA GCCTGGGTCA CTCCAGGAGC GGGACGCCCG GTGGGAACGG ACTCACTCCG 
GGAGGTTTAA GTGGCTCCAT CGAGCTCCTG GAGGATCAGT CCATCGGTGC GGATTCAGAC 
TACAGTGCCG CCGGGATGCT GCGTCTGAGC GAAGGGGTCA ACATCAGTGC TCTGGACAGA 
GGTCAGAGGT CGGAGAGTTC AGAAGGGGCT GTAGGTCACC AAGTCACCAA CAACATTAAA 
GACGGTTCCA ATGGAGGTTT GGGAGAAGGA GGAGGTGACG GCACACAGTT AGAAGATAGC 
ATATCTCTCA TTTCAGGGCA ACAGCGTGTG TATGCTCGAA TGAGCGAGTC GCCTGGGCTG 
AGAAGACGGG CAGATCCTGG CGAAGACAGG TTAAGCGCAG GGTCGTTCCT GTCTTATCTG 
CAGGATCAGG GCTCTCCTGG TTGGCATTCG GCTGCAGAAG CGACTCAGAA CAGATTGGCG 
TCTTTGCAAT GCTCCCTTCC TAGGCAGGCA ATAGAGTCTA TGATGTCATC GGTGCGAGCT 
ACGGTGGACT CTGACTCCAA GCAGTCTTGT TTGGGCCAGG AGGCTCTGAT TGAGCCAGTG 
CGGGACATGG GGCACTCTGA GATCATCCAT GGCCATTATC AGGGGACTCA GCCGTTTGAG 
AAAGGACTCG GCTTCCCGCA CAGAACAACC AACATGCGCA CTTGGGAGGA CATGCGTCTG 
AGCGGACAGG GTGATGAGGC TGAGGATCTT CCAGGAGAGC AAGTGAGCGA GGAGCAATTC 
ACAGACGAGC ATGGCAACAT CGTCACCAAA AAGATTGTGA GGAAAGTGGT CCGGAGAGGA 
AAAGGGGAGG AAGGGGGTCA GGAGCTGATC ATTGAAGGTC TGCAGCAGGA TATCAGTGAG 
CCTGACTTTG ATGGAGAACA GTTCATGAGC TATGCCGTCC TGGGACGTGA CAGCAAGCCG 
GATGTTGTGG ATGTGAAGAG AGGAGGTGCT CAGATAGTCA AATGTGCGAG TCTGCGGAGA 
GTAAAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_009302009.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009300284.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X3, mRNA.

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CloneID	ODa41281
Clone ID Related Accession (Same CDS sequence)	XM_009302009.2
Accession Version	XM_009302009.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5889bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ankyrin 1, erythrocytic a isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302009.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAACCA ACACCATCCT TTTTGGAGCT GACGCTGCCA CCAGTTTCCT CCGCGCAGCT 
CGCTCAGGTA ATCTGGAAAA GGCTCTAGAC CACATCAAGA ACGGCATCGA CATCAACACA 
GCCAATCAGA ATGGACTCAA CGGGCTGCAT TTGGCCTCCA AGGAAGGCCA TGTTAAAATG 
GTGCTGGAGC TGCTTCACCA TGGTATTGTA CTTGAGACGA CAACGAAGAA AGGGAACACT 
GCTCTTCATA TAGCGGCGTT GGCTGGCCAG GAGCAGGTGG TCACTGAACT GGTCAACTAT 
GGAGCAAACG TCAACGCTCA GTCTCAGAAG GGCTTCACGC CGCTCTACAT GGCAGCACAA 
GAGAATCATC TGGAGGTTGT GAAGTTTCTG CTGGATAACG GAGCCAATCA GACCATTCCA 
ACTGAGGATG GCTTTACCCC TCTTGCTGTG GCTCTTCAGC AGGGCCATGA GAATGTGGTG 
GCCCTGCTCA TCAACCACGG TACTAAGGGC AAGGTCCGCC TTCCCGCACT GCACATCGCT 
GCCCGAAACG ACGACACCCG CACTGCCGCT GTGCTCTTAC AGAACGACCC CAATCCTGAC 
GTCCTGAGCA AGACAGGCTT CACGCCGCTC CACATCGCAG CACATTATGA AAACCTGAAT 
GTTGCTCAGC TTCTTCTGAA CCGAGGAGCT GATGTGAACT TCACACCTAA GAACGGGATC 
ACACCGCTGC ACATCGCATC CAGGAGAGGG AACGTCATCA TGGTCAGACT TCTGCTGGAC 
CGAGGAGCAA AGATCGATGC CAAGACAAAA GATGAGTTGA CTCCTCTTCA CTGTGCTGCC 
AGAAATGGCC ATGTGAGAAT CATCGAGATC CTTCTGGACC AAGGAGCCCC AATACAGGCC 
AAGACCAAAA ATGGCCTGTC GCCGATCCAC ATGGCAGCCC AGGGCGATCA CTTGGACTGT 
ATCCGACAGC TCTTACAGTA CAACGCAGAG ATCGATGACA TCACTCTGGA CCACCTGACA 
CCTCTGCATG TGGCGGCTCA CTGCGGGCAT CACCGCGTGG CCAAGGTGCT CCTGGATAAA 
GGAGCCAAAC CAAACACCCG CGCTCTGAAT GGATTCACGC CGCTCCACAT TGCCTGTAAG 
AAGAACCACA TGCGTGTAAT GGACCTGCTA TTAAAGCATT CTGCATCCCT GGAAGCTGTA 
ACCGAGTCAG GCTTGACCCC GCTGCATGTG TCCTCCTTCA TGGGTCATCT GAATATAGTC 
AAGATACTGA TGCAAAAAGG AGCTTCGCCG AACGCCTCAA ATGTGAAAGT GGAAACCCCT 
CTGCATATGG CATCTCGAGC TGGACACTGT GAAGTTGCTG AATTCCTATT GCAAAATGCA 
GCTCCAGTTG ATGCCAAAGC CAAGGATGAC CAGACGCCGC TACACTGTGC CTCCCGCATG 
GGCCACAACG AGATGGTGAA GTTGCTTCTG GAGCACAAGG CCAACTCAAA CTCCACCACC 
ACAGCAGGAC ACACACCGCT GCACATCGCG GCCCGCGAGG GACACACACA AACTGCCAGC 
ATCCTCCTGG ACATGAACGC CCAGCTCACC AAAATGACCA AGAAAGGCTT CACTCCTCTT 
CATGTTGCAG CCAAATACGG AAAGGTGGAC GTGGCAGTTC TGCTTCTGGA GAGGGGAGCA 
AATCCAAACG CTGCTGGAAA AGTTGGACTC ACGCCGCTGC ATGTGGCCGT CCATCATAAC 
AATCTAGATG TGGTCAATCT TTTACTCAGC AAAGGAGGAT CACCACACAG CGCCGCTCGG 
AATGGCTATA CTCCGCTCCA CATTGCTTCC AAACAGAACC AGGCGGAGGT GGCGAGCAGT 
TTACTGCAGC ACGGGGCGTC TGCCAATGCT GAGTCTCTGC AAGGGGTCAC ACCTCTACAC 
CTCGCCTCTC AAGAGGGTCA ACCTGACATG GTGTTACTGC TCATCTCCAA GCAGGCCAAT 
GTAAACCTTG GCAATAAGAG CGGTCTGACT CCTCTTCATC TTGTGGCTCA GGAAGGGCAT 
GTTGGGATTG CTGATATACT AGTGAAACAT GGAGCATCTG TTTATGCTGC CACACGGATG 
GGTTACACTC CGCTTCACGT GGCTTGTCAT TATGGGAATA TCAAGATGGT GAAGTTTCTC 
TTACAGCAGC AGGCCAACGT AAACTGCAAG ACCAGGATGA GCTACACGCC GCTACATCAG 
GCTGCTCAGC AGGGTCATAC GGACATCGTG ACGCTTCTGC TGAAACACGG AGCACAACCG 
AATGAAGTTA CATCTAACGG CACCTCTGCT CTTGCCATCG CCAAGCGGCT TGGATACATT 
TCAGTCATCG ATGTGCTCAA ACTGGTCACT GAAGAGACGG TCACTATGAC CACCACTGAA 
AAACATCGCA TGAGTTTCCC TGAAACCGTT GATGAGATCC TGGATGTGTC TGAAGATGAA 
GGCGAGGAGC TGCTGGGAAA CGAGGGCGCC AGGTATTTGA TGAAGATGGA GGACATAAAG 
GACCATGATG AGGATTTCCT TTCCCCCAAG AAAGTTCTAG AGTACGAGGG AGAGTTGGAG 
AATCATTCTC CTGCTATCCC GAGGATCCCG TGTGTGTCTC CTGAGACTGT CATGCTGAAA 
GAGCATGAGA TGGAGCAGCA GCACACTCCA CTTCCATTGC CCAAAGAGTA CGACGAGGAT 
TCTCTGATTC CCAGCAGTCC TGCAACTGAG ACTTCAGACA ATGTCAGTCC GGTTGCCAGT 
CCCATCCACA CCGGTTTTCT GGTGAGCTTC ATGGTGGATG CTCGCGGCGG CTCGATGCGC 
GGCAGCAGAC ACAACGGCCT CCGCGTCATC ATTCCTCCTC GAACCTGCGC CGCTCCCACA 
CGCATCACCT GCCGCCTGGT GAAGCCCCAG AAGCTGCCCA CACCGCCGCC ATTAGTGGAG 
GGAGAGGGTC TCGCCAGCCG CATCATCTCC CTTGGACCCG CCAGCATGCA GTTTTTAGGG 
CCTGTGATTG TGGAGATTCC TCATTTTGCG GCTCTCGGTC GGGGAGATCG GGAACTCGTG 
GTCTTGAGGA GTGAGAACGG ATCGGTCTGG AAGGAACATC GTAACCGATA TGGAGATGAA 
GTGCTAGAGA CCATCCTCAA CGGCATGGAT GAAGATCTGG AGAGTCAGGA GGAGCTCGGG 
AAGAAACGCA TCAGACGGAT CATCTCCACC GACTTCCCGC TCTACTTTGC GGTGGTGTCT 
CGCATCCAGC AGGAGAACGA TCTGATTGGC CCAGAAGGAG GTTTTCTGTC CAGTAAACTG 
GTGCCGATGG TCCAGGCCAC CTTTCCAGAG ACAGCCGTCA CCAAACGCGT GCGTCTGGGA 
CTGCAGGCCC AGCCGGTGCC TGATGAGCTG GTGGCCAAGC TTTTGGGGAA CCAGGCCACG 
TTCAGTCCTG TGGTGACGGT GGAGCCTCGG CGCCGGAAGT TTCACCGTCC CATCGGCCTA 
AGAATCCCAC TGCCACCCTC ATGGAGAGAG AGCCCGCGGG ACGCCGGAGA GGGAGACACC 
ACCAGCCTGC GCCTGCTCTG CAGCGTCATC GGTGGAACAG CTCCTGCCCA GTGGGAAGAT 
ATAACTGGCA CCACCAAACT GGCGTATTCC AATGACTGTG CCAGTTTCAC CACCAACGTC 
TCTGCCCGAT TCTGGCTGGC GGACTGTCCT CGGACTGCGG AGGCCGTGTC CTTTGCCAAC 
CTCCTGTACC GCGAGCTGTC GGCCGTGCCG TACATGGCCA AGTTTGTAGT GTTTGCGAAG 
ATGAATGAAG CTCGCGAGGG TCGACTCCGC TGCTACTGCA TGACGGATGA CAAGATGGAC 
AAAACCCTGG AGCAGCACGA GAACTTCTCG GAAGTGGCTC GCAGCAGAGA CATCGAGGTG 
ATGGAGGGAA TGCCGCTGTA TTTGGAGTGT TCAGGAAACT TGGTTCCTAT AAGAAAAGCA 
GCCCAGCAGC CTCGCTGTTT CAGCTTCCAG GCCTTTAAAG ACAACAGACT GCCTGTGTCC 
GTCAAGGTGA GGGACAGCAG TAAAGACCCC TCTGGGTTTT TGTCTTTCTT GCGCAAGTCA 
ACCAAATATG AAGACAGCCA ACACGTGCTG TGCAACCTCA ACGTGACCAT GCCGCCGTGC 
ATTAAGATTA TGGGCAGCGA TGAGCGGAGG AGGACTCTGA CTCCTCTGGC TCTCAGAGAA 
AGATACAGCG CTCTGAATGA ACCGGCAATG GCATCACTGA GCGCCATGGA AAGGACGGAG 
TTAAAGATGG CTTTAATAGC TGAACAGCTA GGCCTGAGCT GGGCTGAGTT GGGACGGGAA 
CTGCAGTTTA ATGTAGATGA AATAAATAAG ATCCGTGTGG AGAATCCCAA CTCCCTTTTA 
GAGCAGAGCT CAACGCTGCT CAACCTGTGG GCCGCCCGAG AGGGGAAGAG AGCCAAAATG 
GACAGCCTGT ACGTGGCCCT CAAGAGCATC GACCGAGTGG ACATTGTGAA CATGATGGAG 
GGCCAGGGAG GACCACCGGC GGGTCAGCAG GGGGGCTGGG AGCAGGACGC TTCTAGGTCT 
AGGCATCACG ACCGAGACCA TCTCTCACCT GGAATCACAA ATGGTTACGG GCTGGTGGTG 
CAGGAGGAAC TAGTGTCACC GGCCTCCATG CAGTACAGTC TGCCCTCGCC ACTGGGCAAT 
GAGCCCTACT GGCAGGAGGT TTCCAGCATG GAGTGCGCGC CCATCGCCAC CACTGAAGAA 
GACACGCTCA TGGAGATGTC CGAGGTACAG GTTTGGCCCT CAGGAAATAG TCCGTCCATT 
GTGGCTGTAG AGGACTCCTC ATTGGAGTGC AGCAATGCGG ACGACTCCGA GGCGCTGCAG 
AACCGGAGCC TGGGTCACTC CAGGAGCGGG ACGCCCGGTG GGAACGGACT CACTCCGGGA 
GGTTTAAGTG GCTCCATCGA GCTCCTGGAG GATCAGTCCA TCGGTGCGGA TTCAGACTAC 
AGTGCCGCCG GGATGCTGCG TCTGAGCGAA GGGGTCAACA TCAGTGCTCT GGACAGAGGT 
CAGAGGTCGG AGAGTTCAGA AGGGGCTGTA GGTCACCAAG TCACCAACAA CATTAAAGAC 
GGTTCCAATG GAGGTTTGGG AGAAGGAGGA GGTGACGGCA CACAGTTAGA AGATAGCATA 
TCTCTCATTT CAGGGCAACA GCGTGTGTAT GCTCGAATGA GCGAGTCGCC TGGGCTGAGA 
AGACGGGCAG ATCCTGGCGA AGACAGGTTA AGCGCAGGGT CGTTCCTGTC TTATCTGCAG 
GATCAGGGCT CTCCTGGTTG GCATTCGGCT GCAGAAGCGA CTCAGAACAG ATTGGCGTCT 
TTGCAATGCT CCCTTCCTAG GCAGGCAATA GAGTCTATGA TGTCATCGGT GCGAGCTACG 
GTGGACTCTG ACTCCAAGCA GTCTTGTTTG GGCCAGGAGG CTCTGATTGA GCCAGTGCGG 
GACATGGGGC ACTCTGAGAT CATCCATGGC CATTATCAGG GGACTCAGCC GTTTGAGAAA 
GGACTCGGCT TCCCGCACAG AACAACCAAC ATGCGCACTT GGGAGGACAT GCGTCTGAGC 
GGACAGGGTG ATGAGGCTGA GGATCTTCCA GGAGAGCAAG TGAGCGAGGA GCAATTCACA 
GACGAGCATG GCAACATCGT CACCAAAAAG ATTGTGAGGA AAGTGGTCCG GAGAGGAAAA 
GGGGAGGAAG GGGGTCAGGA GCTGATCATT GAAGGTCTGC AGCAGGATAT CAGTGAGCCT 
GACTTTGATG GAGAACAGTT CATGAGCTAT GCCGTCCTGG GACGTGACAG CAAGCCGGAT 
GTTGTGGATG TGAAGAGAGG AGGTGCTCAG ATAGTCAAAT GTGCGAGTCT GCGGAGAGTA 
AAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300284.1
CDS	343..6231
Translation	MPTNTILFGADAATSFLRAARSGNLEKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLINHGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGLTPLHVSSFMGHLNIVKILMQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGANPNAAGKVGLTPLHVAVHHNNLDVVNLLLSKGGSPHSAARNGYTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPLHLASQEGQPDMVLLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKHGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSNGTSALAIAKRLGYISVIDVLKLVTEETVTMTTTEKHRMSFPETVDEILDVSEDEGEELLGNEGARYLMKMEDIKDHDEDFLSPKKVLEYEGELENHSPAIPRIPCVSPETVMLKEHEMEQQHTPLPLPKEYDEDSLIPSSPATETSDNVSPVASPIHTGFLVSFMVDARGGSMRGSRHNGLRVIIPPRTCAAPTRITCRLVKPQKLPTPPPLVEGEGLASRIISLGPASMQFLGPVIVEIPHFAALGRGDRELVVLRSENGSVWKEHRNRYGDEVLETILNGMDEDLESQEELGKKRIRRIISTDFPLYFAVVSRIQQENDLIGPEGGFLSSKLVPMVQATFPETAVTKRVRLGLQAQPVPDELVAKLLGNQATFSPVVTVEPRRRKFHRPIGLRIPLPPSWRESPRDAGEGDTTSLRLLCSVIGGTAPAQWEDITGTTKLAYSNDCASFTTNVSARFWLADCPRTAEAVSFANLLYRELSAVPYMAKFVVFAKMNEAREGRLRCYCMTDDKMDKTLEQHENFSEVARSRDIEVMEGMPLYLECSGNLVPIRKAAQQPRCFSFQAFKDNRLPVSVKVRDSSKDPSGFLSFLRKSTKYEDSQHVLCNLNVTMPPCIKIMGSDERRRTLTPLALRERYSALNEPAMASLSAMERTELKMALIAEQLGLSWAELGRELQFNVDEINKIRVENPNSLLEQSSTLLNLWAAREGKRAKMDSLYVALKSIDRVDIVNMMEGQGGPPAGQQGGWEQDASRSRHHDRDHLSPGITNGYGLVVQEELVSPASMQYSLPSPLGNEPYWQEVSSMECAPIATTEEDTLMEMSEVQVWPSGNSPSIVAVEDSSLECSNADDSEALQNRSLGHSRSGTPGGNGLTPGGLSGSIELLEDQSIGADSDYSAAGMLRLSEGVNISALDRGQRSESSEGAVGHQVTNNIKDGSNGGLGEGGGDGTQLEDSISLISGQQRVYARMSESPGLRRRADPGEDRLSAGSFLSYLQDQGSPGWHSAAEATQNRLASLQCSLPRQAIESMMSSVRATVDSDSKQSCLGQEALIEPVRDMGHSEIIHGHYQGTQPFEKGLGFPHRTTNMRTWEDMRLSGQGDEAEDLPGEQVSEEQFTDEHGNIVTKKIVRKVVRRGKGEEGGQELIIEGLQQDISEPDFDGEQFMSYAVLGRDSKPDVVDVKRGGAQIVKCASLRRVKQ

Target ORF information:

RefSeq Version	XM_009302009.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302009.2

ORF Insert Sequence:

ATGCCAACCA ACACCATCCT TTTTGGAGCT GACGCTGCCA CCAGTTTCCT CCGCGCAGCT 
CGCTCAGGTA ATCTGGAAAA GGCTCTAGAC CACATCAAGA ACGGCATCGA CATCAACACA 
GCCAATCAGA ATGGACTCAA CGGGCTGCAT TTGGCCTCCA AGGAAGGCCA TGTTAAAATG 
GTGCTGGAGC TGCTTCACCA TGGTATTGTA CTTGAGACGA CAACGAAGAA AGGGAACACT 
GCTCTTCATA TAGCGGCGTT GGCTGGCCAG GAGCAGGTGG TCACTGAACT GGTCAACTAT 
GGAGCAAACG TCAACGCTCA GTCTCAGAAG GGCTTCACGC CGCTCTACAT GGCAGCACAA 
GAGAATCATC TGGAGGTTGT GAAGTTTCTG CTGGATAACG GAGCCAATCA GACCATTCCA 
ACTGAGGATG GCTTTACCCC TCTTGCTGTG GCTCTTCAGC AGGGCCATGA GAATGTGGTG 
GCCCTGCTCA TCAACCACGG TACTAAGGGC AAGGTCCGCC TTCCCGCACT GCACATCGCT 
GCCCGAAACG ACGACACCCG CACTGCCGCT GTGCTCTTAC AGAACGACCC CAATCCTGAC 
GTCCTGAGCA AGACAGGCTT CACGCCGCTC CACATCGCAG CACATTATGA AAACCTGAAT 
GTTGCTCAGC TTCTTCTGAA CCGAGGAGCT GATGTGAACT TCACACCTAA GAACGGGATC 
ACACCGCTGC ACATCGCATC CAGGAGAGGG AACGTCATCA TGGTCAGACT TCTGCTGGAC 
CGAGGAGCAA AGATCGATGC CAAGACAAAA GATGAGTTGA CTCCTCTTCA CTGTGCTGCC 
AGAAATGGCC ATGTGAGAAT CATCGAGATC CTTCTGGACC AAGGAGCCCC AATACAGGCC 
AAGACCAAAA ATGGCCTGTC GCCGATCCAC ATGGCAGCCC AGGGCGATCA CTTGGACTGT 
ATCCGACAGC TCTTACAGTA CAACGCAGAG ATCGATGACA TCACTCTGGA CCACCTGACA 
CCTCTGCATG TGGCGGCTCA CTGCGGGCAT CACCGCGTGG CCAAGGTGCT CCTGGATAAA 
GGAGCCAAAC CAAACACCCG CGCTCTGAAT GGATTCACGC CGCTCCACAT TGCCTGTAAG 
AAGAACCACA TGCGTGTAAT GGACCTGCTA TTAAAGCATT CTGCATCCCT GGAAGCTGTA 
ACCGAGTCAG GCTTGACCCC GCTGCATGTG TCCTCCTTCA TGGGTCATCT GAATATAGTC 
AAGATACTGA TGCAAAAAGG AGCTTCGCCG AACGCCTCAA ATGTGAAAGT GGAAACCCCT 
CTGCATATGG CATCTCGAGC TGGACACTGT GAAGTTGCTG AATTCCTATT GCAAAATGCA 
GCTCCAGTTG ATGCCAAAGC CAAGGATGAC CAGACGCCGC TACACTGTGC CTCCCGCATG 
GGCCACAACG AGATGGTGAA GTTGCTTCTG GAGCACAAGG CCAACTCAAA CTCCACCACC 
ACAGCAGGAC ACACACCGCT GCACATCGCG GCCCGCGAGG GACACACACA AACTGCCAGC 
ATCCTCCTGG ACATGAACGC CCAGCTCACC AAAATGACCA AGAAAGGCTT CACTCCTCTT 
CATGTTGCAG CCAAATACGG AAAGGTGGAC GTGGCAGTTC TGCTTCTGGA GAGGGGAGCA 
AATCCAAACG CTGCTGGAAA AGTTGGACTC ACGCCGCTGC ATGTGGCCGT CCATCATAAC 
AATCTAGATG TGGTCAATCT TTTACTCAGC AAAGGAGGAT CACCACACAG CGCCGCTCGG 
AATGGCTATA CTCCGCTCCA CATTGCTTCC AAACAGAACC AGGCGGAGGT GGCGAGCAGT 
TTACTGCAGC ACGGGGCGTC TGCCAATGCT GAGTCTCTGC AAGGGGTCAC ACCTCTACAC 
CTCGCCTCTC AAGAGGGTCA ACCTGACATG GTGTTACTGC TCATCTCCAA GCAGGCCAAT 
GTAAACCTTG GCAATAAGAG CGGTCTGACT CCTCTTCATC TTGTGGCTCA GGAAGGGCAT 
GTTGGGATTG CTGATATACT AGTGAAACAT GGAGCATCTG TTTATGCTGC CACACGGATG 
GGTTACACTC CGCTTCACGT GGCTTGTCAT TATGGGAATA TCAAGATGGT GAAGTTTCTC 
TTACAGCAGC AGGCCAACGT AAACTGCAAG ACCAGGATGA GCTACACGCC GCTACATCAG 
GCTGCTCAGC AGGGTCATAC GGACATCGTG ACGCTTCTGC TGAAACACGG AGCACAACCG 
AATGAAGTTA CATCTAACGG CACCTCTGCT CTTGCCATCG CCAAGCGGCT TGGATACATT 
TCAGTCATCG ATGTGCTCAA ACTGGTCACT GAAGAGACGG TCACTATGAC CACCACTGAA 
AAACATCGCA TGAGTTTCCC TGAAACCGTT GATGAGATCC TGGATGTGTC TGAAGATGAA 
GGCGAGGAGC TGCTGGGAAA CGAGGGCGCC AGGTATTTGA TGAAGATGGA GGACATAAAG 
GACCATGATG AGGATTTCCT TTCCCCCAAG AAAGTTCTAG AGTACGAGGG AGAGTTGGAG 
AATCATTCTC CTGCTATCCC GAGGATCCCG TGTGTGTCTC CTGAGACTGT CATGCTGAAA 
GAGCATGAGA TGGAGCAGCA GCACACTCCA CTTCCATTGC CCAAAGAGTA CGACGAGGAT 
TCTCTGATTC CCAGCAGTCC TGCAACTGAG ACTTCAGACA ATGTCAGTCC GGTTGCCAGT 
CCCATCCACA CCGGTTTTCT GGTGAGCTTC ATGGTGGATG CTCGCGGCGG CTCGATGCGC 
GGCAGCAGAC ACAACGGCCT CCGCGTCATC ATTCCTCCTC GAACCTGCGC CGCTCCCACA 
CGCATCACCT GCCGCCTGGT GAAGCCCCAG AAGCTGCCCA CACCGCCGCC ATTAGTGGAG 
GGAGAGGGTC TCGCCAGCCG CATCATCTCC CTTGGACCCG CCAGCATGCA GTTTTTAGGG 
CCTGTGATTG TGGAGATTCC TCATTTTGCG GCTCTCGGTC GGGGAGATCG GGAACTCGTG 
GTCTTGAGGA GTGAGAACGG ATCGGTCTGG AAGGAACATC GTAACCGATA TGGAGATGAA 
GTGCTAGAGA CCATCCTCAA CGGCATGGAT GAAGATCTGG AGAGTCAGGA GGAGCTCGGG 
AAGAAACGCA TCAGACGGAT CATCTCCACC GACTTCCCGC TCTACTTTGC GGTGGTGTCT 
CGCATCCAGC AGGAGAACGA TCTGATTGGC CCAGAAGGAG GTTTTCTGTC CAGTAAACTG 
GTGCCGATGG TCCAGGCCAC CTTTCCAGAG ACAGCCGTCA CCAAACGCGT GCGTCTGGGA 
CTGCAGGCCC AGCCGGTGCC TGATGAGCTG GTGGCCAAGC TTTTGGGGAA CCAGGCCACG 
TTCAGTCCTG TGGTGACGGT GGAGCCTCGG CGCCGGAAGT TTCACCGTCC CATCGGCCTA 
AGAATCCCAC TGCCACCCTC ATGGAGAGAG AGCCCGCGGG ACGCCGGAGA GGGAGACACC 
ACCAGCCTGC GCCTGCTCTG CAGCGTCATC GGTGGAACAG CTCCTGCCCA GTGGGAAGAT 
ATAACTGGCA CCACCAAACT GGCGTATTCC AATGACTGTG CCAGTTTCAC CACCAACGTC 
TCTGCCCGAT TCTGGCTGGC GGACTGTCCT CGGACTGCGG AGGCCGTGTC CTTTGCCAAC 
CTCCTGTACC GCGAGCTGTC GGCCGTGCCG TACATGGCCA AGTTTGTAGT GTTTGCGAAG 
ATGAATGAAG CTCGCGAGGG TCGACTCCGC TGCTACTGCA TGACGGATGA CAAGATGGAC 
AAAACCCTGG AGCAGCACGA GAACTTCTCG GAAGTGGCTC GCAGCAGAGA CATCGAGGTG 
ATGGAGGGAA TGCCGCTGTA TTTGGAGTGT TCAGGAAACT TGGTTCCTAT AAGAAAAGCA 
GCCCAGCAGC CTCGCTGTTT CAGCTTCCAG GCCTTTAAAG ACAACAGACT GCCTGTGTCC 
GTCAAGGTGA GGGACAGCAG TAAAGACCCC TCTGGGTTTT TGTCTTTCTT GCGCAAGTCA 
ACCAAATATG AAGACAGCCA ACACGTGCTG TGCAACCTCA ACGTGACCAT GCCGCCGTGC 
ATTAAGATTA TGGGCAGCGA TGAGCGGAGG AGGACTCTGA CTCCTCTGGC TCTCAGAGAA 
AGATACAGCG CTCTGAATGA ACCGGCAATG GCATCACTGA GCGCCATGGA AAGGACGGAG 
TTAAAGATGG CTTTAATAGC TGAACAGCTA GGCCTGAGCT GGGCTGAGTT GGGACGGGAA 
CTGCAGTTTA ATGTAGATGA AATAAATAAG ATCCGTGTGG AGAATCCCAA CTCCCTTTTA 
GAGCAGAGCT CAACGCTGCT CAACCTGTGG GCCGCCCGAG AGGGGAAGAG AGCCAAAATG 
GACAGCCTGT ACGTGGCCCT CAAGAGCATC GACCGAGTGG ACATTGTGAA CATGATGGAG 
GGCCAGGGAG GACCACCGGC GGGTCAGCAG GGGGGCTGGG AGCAGGACGC TTCTAGGTCT 
AGGCATCACG ACCGAGACCA TCTCTCACCT GGAATCACAA ATGGTTACGG GCTGGTGGTG 
CAGGAGGAAC TAGTGTCACC GGCCTCCATG CAGTACAGTC TGCCCTCGCC ACTGGGCAAT 
GAGCCCTACT GGCAGGAGGT TTCCAGCATG GAGTGCGCGC CCATCGCCAC CACTGAAGAA 
GACACGCTCA TGGAGATGTC CGAGGTACAG GTTTGGCCCT CAGGAAATAG TCCGTCCATT 
GTGGCTGTAG AGGACTCCTC ATTGGAGTGC AGCAATGCGG ACGACTCCGA GGCGCTGCAG 
AACCGGAGCC TGGGTCACTC CAGGAGCGGG ACGCCCGGTG GGAACGGACT CACTCCGGGA 
GGTTTAAGTG GCTCCATCGA GCTCCTGGAG GATCAGTCCA TCGGTGCGGA TTCAGACTAC 
AGTGCCGCCG GGATGCTGCG TCTGAGCGAA GGGGTCAACA TCAGTGCTCT GGACAGAGGT 
CAGAGGTCGG AGAGTTCAGA AGGGGCTGTA GGTCACCAAG TCACCAACAA CATTAAAGAC 
GGTTCCAATG GAGGTTTGGG AGAAGGAGGA GGTGACGGCA CACAGTTAGA AGATAGCATA 
TCTCTCATTT CAGGGCAACA GCGTGTGTAT GCTCGAATGA GCGAGTCGCC TGGGCTGAGA 
AGACGGGCAG ATCCTGGCGA AGACAGGTTA AGCGCAGGGT CGTTCCTGTC TTATCTGCAG 
GATCAGGGCT CTCCTGGTTG GCATTCGGCT GCAGAAGCGA CTCAGAACAG ATTGGCGTCT 
TTGCAATGCT CCCTTCCTAG GCAGGCAATA GAGTCTATGA TGTCATCGGT GCGAGCTACG 
GTGGACTCTG ACTCCAAGCA GTCTTGTTTG GGCCAGGAGG CTCTGATTGA GCCAGTGCGG 
GACATGGGGC ACTCTGAGAT CATCCATGGC CATTATCAGG GGACTCAGCC GTTTGAGAAA 
GGACTCGGCT TCCCGCACAG AACAACCAAC ATGCGCACTT GGGAGGACAT GCGTCTGAGC 
GGACAGGGTG ATGAGGCTGA GGATCTTCCA GGAGAGCAAG TGAGCGAGGA GCAATTCACA 
GACGAGCATG GCAACATCGT CACCAAAAAG ATTGTGAGGA AAGTGGTCCG GAGAGGAAAA 
GGGGAGGAAG GGGGTCAGGA GCTGATCATT GAAGGTCTGC AGCAGGATAT CAGTGAGCCT 
GACTTTGATG GAGAACAGTT CATGAGCTAT GCCGTCCTGG GACGTGACAG CAAGCCGGAT 
GTTGTGGATG TGAAGAGAGG AGGTGCTCAG ATAGTCAAAT GTGCGAGTCT GCGGAGAGTA 
AAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_009302010.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009300285.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X4, mRNA.

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CloneID	ODa41282
Clone ID Related Accession (Same CDS sequence)	XM_009302010.2
Accession Version	XM_009302010.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5877bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ankyrin 1, erythrocytic a isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302010.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAACCA ACACCATCCT TTTTGGAGCT GACGCTGCCA CCAGTTTCCT CCGCGCAGCT 
CGCTCAGGTA ATCTGGAAAA GGCTCTAGAC CACATCAAGA ACGGCATCGA CATCAACACA 
GCCAATCAGA ATGGACTCAA CGGGCTGCAT TTGGCCTCCA AGGAAGGCCA TGTTAAAATG 
GTGCTGGAGC TGCTTCACCA TGGTATTGTA CTTGAGACGA CAACGAAGAA AGGGAACACT 
GCTCTTCATA TAGCGGCGTT GGCTGGCCAG GAGCAGGTGG TCACTGAACT GGTCAACTAT 
GGAGCAAACG TCAACGCTCA GTCTCAGAAG GGCTTCACGC CGCTCTACAT GGCAGCACAA 
GAGAATCATC TGGAGGTTGT GAAGTTTCTG CTGGATAACG GAGCCAATCA GACCATTCCA 
ACTGAGGATG GCTTTACCCC TCTTGCTGTG GCTCTTCAGC AGGGCCATGA GAATGTGGTG 
GCCCTGCTCA TCAACCACGG TACTAAGGGC AAGGTCCGCC TTCCCGCACT GCACATCGCT 
GCCCGAAACG ACGACACCCG CACTGCCGCT GTGCTCTTAC AGAACGACCC CAATCCTGAC 
GTCCTGAGCA AGACAGGCTT CACGCCGCTC CACATCGCAG CACATTATGA AAACCTGAAT 
GTTGCTCAGC TTCTTCTGAA CCGAGGAGCT GATGTGAACT TCACACCTAA GAACGGGATC 
ACACCGCTGC ACATCGCATC CAGGAGAGGG AACGTCATCA TGGTCAGACT TCTGCTGGAC 
CGAGGAGCAA AGATCGATGC CAAGACAAAA GATGAGTTGA CTCCTCTTCA CTGTGCTGCC 
AGAAATGGCC ATGTGAGAAT CATCGAGATC CTTCTGGACC AAGGAGCCCC AATACAGGCC 
AAGACCAAAA ATGGCCTGTC GCCGATCCAC ATGGCAGCCC AGGGCGATCA CTTGGACTGT 
ATCCGACAGC TCTTACAGTA CAACGCAGAG ATCGATGACA TCACTCTGGA CCACCTGACA 
CCTCTGCATG TGGCGGCTCA CTGCGGGCAT CACCGCGTGG CCAAGGTGCT CCTGGATAAA 
GGAGCCAAAC CAAACACCCG CGCTCTGAAT GGATTCACGC CGCTCCACAT TGCCTGTAAG 
AAGAACCACA TGCGTGTAAT GGACCTGCTA TTAAAGCATT CTGCATCCCT GGAAGCTGTA 
ACCGAGTCAG GCTTGACCCC GCTGCATGTG TCCTCCTTCA TGGGTCATCT GAATATAGTC 
AAGATACTGA TGCAAAAAGG AGCTTCGCCG AACGCCTCAA ATGTGAAAGT GGAAACCCCT 
CTGCATATGG CATCTCGAGC TGGACACTGT GAAGTTGCTG AATTCCTATT GCAAAATGCA 
GCTCCAGTTG ATGCCAAAGC CAAGGATGAC CAGACGCCGC TACACTGTGC CTCCCGCATG 
GGCCACAACG AGATGGTGAA GTTGCTTCTG GAGCACAAGG CCAACTCAAA CTCCACCACC 
ACAGCAGGAC ACACACCGCT GCACATCGCG GCCCGCGAGG GACACACACA AACTGCCAGC 
ATCCTCCTGG ACATGAACGC CCAGCTCACC AAAATGACCA AGAAAGGCTT CACTCCTCTT 
CATGTTGCAG CCAAATACGG AAAGGTGGAC GTGGCAGTTC TGCTTCTGGA GAGGGGAGCA 
AATCCAAACG CTGCTGGAAA AGTTGGACTC ACGCCGCTGC ATGTGGCCGT CCATCATAAC 
AATCTAGATG TGGTCAATCT TTTACTCAGC AAAGGAGGAT CACCACACAG CGCCGCTCGG 
AATGGCTATA CTCCGCTCCA CATTGCTTCC AAACAGAACC AGGCGGAGGT GGCGAGCAGT 
TTACTGCAGC ACGGGGCGTC TGCCAATGCT GAGTCTCTGC AAGGGGTCAC ACCTCTACAC 
CTCGCCTCTC AAGAGGGTCA ACCTGACATG GTGTTACTGC TCATCTCCAA GCAGGCCAAT 
GTAAACCTTG GCAATAAGAG CGGTCTGACT CCTCTTCATC TTGTGGCTCA GGAAGGGCAT 
GTTGGGATTG CTGATATACT AGTGAAACAT GGAGCATCTG TTTATGCTGC CACACGGATG 
GGTTACACTC CGCTTCACGT GGCTTGTCAT TATGGGAATA TCAAGATGGT GAAGTTTCTC 
TTACAGCAGC AGGCCAACGT AAACTGCAAG ACCAGGATGA GCTACACGCC GCTACATCAG 
GCTGCTCAGC AGGGTCATAC GGACATCGTG ACGCTTCTGC TGAAACACGG AGCACAACCG 
AATGAAGTTA CATCTAACGG CACCTCTGCT CTTGCCATCG CCAAGCGGCT TGGATACATT 
TCAGTCATCG ATGTGCTCAA ACTGGTCACT GAAGAGACGG TCACTATGAC CACCACTGAA 
AAACATCGCA TGAGTTTCCC TGAAACCGTT GATGAGATCC TGGATGTGTC TGAAGATGAA 
GGAATTGCAC AGCTAACATT AGGCGAGGAG CTGCTGGGAA ACGAGGGCGC CAGGTATTTG 
ATGAAGATGG AGGACATAAA GGACCATGAT GAGGATTTCC TTTCCCCCAA GAAAGTTCTA 
GAGTACGAGG GAGAGTTGGA GAATCATTCT CCTGCTATCC CGAGGATCCC GTGTGTGTCT 
CCTGAGACTG TCATGCTGAA AGAGCATGAG ATGGAGCAGC AGCACACTCC ACTTCCATTG 
CCCAAAGAGT ACGACGAGGA TTCTCTGATT CCCAGCAGTC CTGCAACTGA GACTTCAGAC 
AATGTCAGTC CGGTTGCCAG TCCCATCCAC ACCGGTTTTC TGGTGAGCTT CATGGTGGAT 
GCTCGCGGCG GCTCGATGCG CGGCAGCAGA CACAACGGCC TCCGCGTCAT CATTCCTCCT 
CGAACCTGCG CCGCTCCCAC ACGCATCACC TGCCGCCTGG TGAAGCCCCA GAAGCTGCCC 
ACACCGCCGC CATTAGTGGA GGGAGAGGGT CTCGCCAGCC GCATCATCTC CCTTGGACCC 
GCCAGCATGC AGTTTTTAGG GCCTGTGATT GTGGAGATTC CTCATTTTGC GGCTCTCGGT 
CGGGGAGATC GGGAACTCGT GGTCTTGAGG AGTGAGAACG GATCGGTCTG GAAGGAACAT 
CGTAACCGAT ATGGAGATGA AGTGCTAGAG ACCATCCTCA ACGGCATGGA TGAAGATCTG 
GAGAGTCAGG AGGAGCTCGG GAAGAAACGC ATCAGACGGA TCATCTCCAC CGACTTCCCG 
CTCTACTTTG CGGTGGTGTC TCGCATCCAG CAGGAGAACG ATCTGATTGG CCCAGAAGGA 
GGTTTTCTGT CCAGTAAACT GGTGCCGATG GTCCAGGCCA CCTTTCCAGA GACAGCCGTC 
ACCAAACGCG TGCGTCTGGG ACTGCAGGCC CAGCCGGTGC CTGATGAGCT GGTGGCCAAG 
CTTTTGGGGA ACCAGGCCAC GTTCAGTCCT GTGGTGACGG TGGAGCCTCG GCGCCGGAAG 
TTTCACCGTC CCATCGGCCT AAGAATCCCA CTGCCACCCT CATGGAGAGA GAGCCCGCGG 
GACGCCGGAG AGGGAGACAC CACCAGCCTG CGCCTGCTCT GCAGCGTCAT CGGTGGAACA 
GCTCCTGCCC AGTGGGAAGA TATAACTGGC ACCACCAAAC TGGCGTATTC CAATGACTGT 
GCCAGTTTCA CCACCAACGT CTCTGCCCGA TTCTGGCTGG CGGACTGTCC TCGGACTGCG 
GAGGCCGTGT CCTTTGCCAA CCTCCTGTAC CGCGAGCTGT CGGCCGTGCC GTACATGGCC 
AAGTTTGTAG TGTTTGCGAA GATGAATGAA GCTCGCGAGG GTCGACTCCG CTGCTACTGC 
ATGACGGATG ACAAGATGGA CAAAACCCTG GAGCAGCACG AGAACTTCTC GGAAGTGGCT 
CGCAGCAGAG ACATCGAGGT GATGGAGGGA ATGCCGCTGT ATTTGGAGTG TTCAGGAAAC 
TTGGTTCCTA TAAGAAAAGC AGCCCAGCAG CCTCGCTGTT TCAGCTTCCA GGCCTTTAAA 
GACAACAGAC TGCCTGTGTC CGTCAAGGTG AGGGACAGCA GTAAAGACCC CTCTGGGTTT 
TTGTCTTTCT TGCGCAAGTC AACCAAATAT GAAGACAGCC AACACGTGCT GTGCAACCTC 
AACGTGACCA TGCCGCCGTG CATTAAGATT ATGGGCAGCG ATGAGCGGAG GAGGACTCTG 
ACTCCTCTGG CTCTCAGAGA AAGATACAGC GCTCTGAATG AACCGGCAAT GGCATCACTG 
AGCGCCATGG AAAGGACGGA GTTAAAGATG GCTTTAATAG CTGAACAGCT AGGCCTGAGC 
TGGGCTGAGT TGGGACGGGA ACTGCAGTTT AATGTAGATG AAATAAATAA GATCCGTGTG 
GAGAATCCCA ACTCCCTTTT AGAGCAGAGC TCAACGCTGC TCAACCTGTG GGCCGCCCGA 
GAGGGGAAGA GAGCCAAAAT GGACAGCCTG TACGTGGCCC TCAAGAGCAT CGACCGAGTG 
GACATTGTGA ACATGATGGA GGGCCAGGGA GGACCACCGG CGGGTCAGCA GGGGGGCTGG 
GAGCAGGACG CTTCTAGGTC TAGGCATCAC GACCGAGACC ATCTCTCACC TGGAATCACA 
AATGGTTACG GGCTGGTGGT GCAGGAGGAA CTAGTGTCAC CGGCCTCCAT GCAGTACAGT 
CTGCCCTCGC CACTGGGCAA TGAGCCCTAC TGGCAGGAGG TTTCCAGCAT GGAGTGCGCG 
CCCATCGCCA CCACTGAAGA AGACACGCTC ATGGAGATGT CCGAGGTACA GGTTTGGCCC 
TCAGGAAATA GTCCGTCCAT TGTGGCTGTA GAGGACTCCT CATTGGAGTG CAGCAATGCG 
GACGACTCCG AGGCGCTGCA GAACCGGAGC CTGGGTCACT CCAGGAGCGG GACGCCCGGT 
GGGAACGGAC TCACTCCGGG AGGTTTAAGT GGCTCCATCG AGCTCCTGGA GGATCAGTCC 
ATCGGTGCGG ATTCAGACTA CAGTGCCGCC GGGATGCTGC GTCTGAGCGA AGGGGTCAAC 
ATCAGTGCTC TGGACAGAGG TCAGAGGTCG GAGAGTTCAG AAGGGGCTGT AGGTCACCAA 
GTCACCAACA ACATTAAAGA CGGTTCCAAT GGAGGTTTGG GAGAAGGAGG AGGTGACGGC 
ACACAGTTAG AAGATAGCAT ATCTCTCATT TCAGGGCAAC AGCGTGTGTA TGCTCGAATG 
AGCGAGTCGC CTGGGCTGAG AAGACGGGCA GATCCTGGCG AAGACAGGTT AAGCGCAGGG 
TCGTTCCTGT CTTATCTGCA GGATCAGGGC TCTCCTGGTT GGCATTCGGC TGCAGAAGCG 
ACTCAGAACA GATTGGCGTC TTTGCAATGC TCCCTTCCTA GGCAGGCAAT AGAGTCTATG 
ATGTCATCGG TGCGAGCTAC GGTGGACTCT GACTCCAAGC AGTCTTGTTT GGGCCAGGAG 
GCTCTGATTG AGCCAGTGCG GGACATGGGG CACTCTGAGA TCATCCATGG CCATTATCAG 
GGGACTCAGC CGTTTGAGAA AGGACTCGGC TTCCCGCACA GAACAACCAA CATGCGCACT 
TGGGAGGACA TGCGTCTGAG CGGACAGGGT GATGAGGCTG AGGATCTTCC AGGAGAGCAA 
GTGAGCGAGG AGCAATTCAC AGACGAGCAT GGCAACATCG TCACCAAAAA GATTGTGAGG 
AAAGTGGTCC GGAGAGGAAA AGGGGAGGAA GGGGGTCAGG AGCTGATCAT TGAAGGTCTG 
CAGCAGGATA TCAGTGAGCC TGACTTTGAT GGAGAACAGT TCATGAGCTA TGCCGTCCTG 
GGACGTGACA GCAAGGACTC GACTCCTTCA CCCAAACCAT CCTTCACTGA CTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300285.1
CDS	343..6219
Translation	MPTNTILFGADAATSFLRAARSGNLEKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLINHGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGLTPLHVSSFMGHLNIVKILMQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGANPNAAGKVGLTPLHVAVHHNNLDVVNLLLSKGGSPHSAARNGYTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPLHLASQEGQPDMVLLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKHGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSNGTSALAIAKRLGYISVIDVLKLVTEETVTMTTTEKHRMSFPETVDEILDVSEDEGIAQLTLGEELLGNEGARYLMKMEDIKDHDEDFLSPKKVLEYEGELENHSPAIPRIPCVSPETVMLKEHEMEQQHTPLPLPKEYDEDSLIPSSPATETSDNVSPVASPIHTGFLVSFMVDARGGSMRGSRHNGLRVIIPPRTCAAPTRITCRLVKPQKLPTPPPLVEGEGLASRIISLGPASMQFLGPVIVEIPHFAALGRGDRELVVLRSENGSVWKEHRNRYGDEVLETILNGMDEDLESQEELGKKRIRRIISTDFPLYFAVVSRIQQENDLIGPEGGFLSSKLVPMVQATFPETAVTKRVRLGLQAQPVPDELVAKLLGNQATFSPVVTVEPRRRKFHRPIGLRIPLPPSWRESPRDAGEGDTTSLRLLCSVIGGTAPAQWEDITGTTKLAYSNDCASFTTNVSARFWLADCPRTAEAVSFANLLYRELSAVPYMAKFVVFAKMNEAREGRLRCYCMTDDKMDKTLEQHENFSEVARSRDIEVMEGMPLYLECSGNLVPIRKAAQQPRCFSFQAFKDNRLPVSVKVRDSSKDPSGFLSFLRKSTKYEDSQHVLCNLNVTMPPCIKIMGSDERRRTLTPLALRERYSALNEPAMASLSAMERTELKMALIAEQLGLSWAELGRELQFNVDEINKIRVENPNSLLEQSSTLLNLWAAREGKRAKMDSLYVALKSIDRVDIVNMMEGQGGPPAGQQGGWEQDASRSRHHDRDHLSPGITNGYGLVVQEELVSPASMQYSLPSPLGNEPYWQEVSSMECAPIATTEEDTLMEMSEVQVWPSGNSPSIVAVEDSSLECSNADDSEALQNRSLGHSRSGTPGGNGLTPGGLSGSIELLEDQSIGADSDYSAAGMLRLSEGVNISALDRGQRSESSEGAVGHQVTNNIKDGSNGGLGEGGGDGTQLEDSISLISGQQRVYARMSESPGLRRRADPGEDRLSAGSFLSYLQDQGSPGWHSAAEATQNRLASLQCSLPRQAIESMMSSVRATVDSDSKQSCLGQEALIEPVRDMGHSEIIHGHYQGTQPFEKGLGFPHRTTNMRTWEDMRLSGQGDEAEDLPGEQVSEEQFTDEHGNIVTKKIVRKVVRRGKGEEGGQELIIEGLQQDISEPDFDGEQFMSYAVLGRDSKDSTPSPKPSFTDS

Target ORF information:

RefSeq Version	XM_009302010.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302010.2

ORF Insert Sequence:

ATGCCAACCA ACACCATCCT TTTTGGAGCT GACGCTGCCA CCAGTTTCCT CCGCGCAGCT 
CGCTCAGGTA ATCTGGAAAA GGCTCTAGAC CACATCAAGA ACGGCATCGA CATCAACACA 
GCCAATCAGA ATGGACTCAA CGGGCTGCAT TTGGCCTCCA AGGAAGGCCA TGTTAAAATG 
GTGCTGGAGC TGCTTCACCA TGGTATTGTA CTTGAGACGA CAACGAAGAA AGGGAACACT 
GCTCTTCATA TAGCGGCGTT GGCTGGCCAG GAGCAGGTGG TCACTGAACT GGTCAACTAT 
GGAGCAAACG TCAACGCTCA GTCTCAGAAG GGCTTCACGC CGCTCTACAT GGCAGCACAA 
GAGAATCATC TGGAGGTTGT GAAGTTTCTG CTGGATAACG GAGCCAATCA GACCATTCCA 
ACTGAGGATG GCTTTACCCC TCTTGCTGTG GCTCTTCAGC AGGGCCATGA GAATGTGGTG 
GCCCTGCTCA TCAACCACGG TACTAAGGGC AAGGTCCGCC TTCCCGCACT GCACATCGCT 
GCCCGAAACG ACGACACCCG CACTGCCGCT GTGCTCTTAC AGAACGACCC CAATCCTGAC 
GTCCTGAGCA AGACAGGCTT CACGCCGCTC CACATCGCAG CACATTATGA AAACCTGAAT 
GTTGCTCAGC TTCTTCTGAA CCGAGGAGCT GATGTGAACT TCACACCTAA GAACGGGATC 
ACACCGCTGC ACATCGCATC CAGGAGAGGG AACGTCATCA TGGTCAGACT TCTGCTGGAC 
CGAGGAGCAA AGATCGATGC CAAGACAAAA GATGAGTTGA CTCCTCTTCA CTGTGCTGCC 
AGAAATGGCC ATGTGAGAAT CATCGAGATC CTTCTGGACC AAGGAGCCCC AATACAGGCC 
AAGACCAAAA ATGGCCTGTC GCCGATCCAC ATGGCAGCCC AGGGCGATCA CTTGGACTGT 
ATCCGACAGC TCTTACAGTA CAACGCAGAG ATCGATGACA TCACTCTGGA CCACCTGACA 
CCTCTGCATG TGGCGGCTCA CTGCGGGCAT CACCGCGTGG CCAAGGTGCT CCTGGATAAA 
GGAGCCAAAC CAAACACCCG CGCTCTGAAT GGATTCACGC CGCTCCACAT TGCCTGTAAG 
AAGAACCACA TGCGTGTAAT GGACCTGCTA TTAAAGCATT CTGCATCCCT GGAAGCTGTA 
ACCGAGTCAG GCTTGACCCC GCTGCATGTG TCCTCCTTCA TGGGTCATCT GAATATAGTC 
AAGATACTGA TGCAAAAAGG AGCTTCGCCG AACGCCTCAA ATGTGAAAGT GGAAACCCCT 
CTGCATATGG CATCTCGAGC TGGACACTGT GAAGTTGCTG AATTCCTATT GCAAAATGCA 
GCTCCAGTTG ATGCCAAAGC CAAGGATGAC CAGACGCCGC TACACTGTGC CTCCCGCATG 
GGCCACAACG AGATGGTGAA GTTGCTTCTG GAGCACAAGG CCAACTCAAA CTCCACCACC 
ACAGCAGGAC ACACACCGCT GCACATCGCG GCCCGCGAGG GACACACACA AACTGCCAGC 
ATCCTCCTGG ACATGAACGC CCAGCTCACC AAAATGACCA AGAAAGGCTT CACTCCTCTT 
CATGTTGCAG CCAAATACGG AAAGGTGGAC GTGGCAGTTC TGCTTCTGGA GAGGGGAGCA 
AATCCAAACG CTGCTGGAAA AGTTGGACTC ACGCCGCTGC ATGTGGCCGT CCATCATAAC 
AATCTAGATG TGGTCAATCT TTTACTCAGC AAAGGAGGAT CACCACACAG CGCCGCTCGG 
AATGGCTATA CTCCGCTCCA CATTGCTTCC AAACAGAACC AGGCGGAGGT GGCGAGCAGT 
TTACTGCAGC ACGGGGCGTC TGCCAATGCT GAGTCTCTGC AAGGGGTCAC ACCTCTACAC 
CTCGCCTCTC AAGAGGGTCA ACCTGACATG GTGTTACTGC TCATCTCCAA GCAGGCCAAT 
GTAAACCTTG GCAATAAGAG CGGTCTGACT CCTCTTCATC TTGTGGCTCA GGAAGGGCAT 
GTTGGGATTG CTGATATACT AGTGAAACAT GGAGCATCTG TTTATGCTGC CACACGGATG 
GGTTACACTC CGCTTCACGT GGCTTGTCAT TATGGGAATA TCAAGATGGT GAAGTTTCTC 
TTACAGCAGC AGGCCAACGT AAACTGCAAG ACCAGGATGA GCTACACGCC GCTACATCAG 
GCTGCTCAGC AGGGTCATAC GGACATCGTG ACGCTTCTGC TGAAACACGG AGCACAACCG 
AATGAAGTTA CATCTAACGG CACCTCTGCT CTTGCCATCG CCAAGCGGCT TGGATACATT 
TCAGTCATCG ATGTGCTCAA ACTGGTCACT GAAGAGACGG TCACTATGAC CACCACTGAA 
AAACATCGCA TGAGTTTCCC TGAAACCGTT GATGAGATCC TGGATGTGTC TGAAGATGAA 
GGAATTGCAC AGCTAACATT AGGCGAGGAG CTGCTGGGAA ACGAGGGCGC CAGGTATTTG 
ATGAAGATGG AGGACATAAA GGACCATGAT GAGGATTTCC TTTCCCCCAA GAAAGTTCTA 
GAGTACGAGG GAGAGTTGGA GAATCATTCT CCTGCTATCC CGAGGATCCC GTGTGTGTCT 
CCTGAGACTG TCATGCTGAA AGAGCATGAG ATGGAGCAGC AGCACACTCC ACTTCCATTG 
CCCAAAGAGT ACGACGAGGA TTCTCTGATT CCCAGCAGTC CTGCAACTGA GACTTCAGAC 
AATGTCAGTC CGGTTGCCAG TCCCATCCAC ACCGGTTTTC TGGTGAGCTT CATGGTGGAT 
GCTCGCGGCG GCTCGATGCG CGGCAGCAGA CACAACGGCC TCCGCGTCAT CATTCCTCCT 
CGAACCTGCG CCGCTCCCAC ACGCATCACC TGCCGCCTGG TGAAGCCCCA GAAGCTGCCC 
ACACCGCCGC CATTAGTGGA GGGAGAGGGT CTCGCCAGCC GCATCATCTC CCTTGGACCC 
GCCAGCATGC AGTTTTTAGG GCCTGTGATT GTGGAGATTC CTCATTTTGC GGCTCTCGGT 
CGGGGAGATC GGGAACTCGT GGTCTTGAGG AGTGAGAACG GATCGGTCTG GAAGGAACAT 
CGTAACCGAT ATGGAGATGA AGTGCTAGAG ACCATCCTCA ACGGCATGGA TGAAGATCTG 
GAGAGTCAGG AGGAGCTCGG GAAGAAACGC ATCAGACGGA TCATCTCCAC CGACTTCCCG 
CTCTACTTTG CGGTGGTGTC TCGCATCCAG CAGGAGAACG ATCTGATTGG CCCAGAAGGA 
GGTTTTCTGT CCAGTAAACT GGTGCCGATG GTCCAGGCCA CCTTTCCAGA GACAGCCGTC 
ACCAAACGCG TGCGTCTGGG ACTGCAGGCC CAGCCGGTGC CTGATGAGCT GGTGGCCAAG 
CTTTTGGGGA ACCAGGCCAC GTTCAGTCCT GTGGTGACGG TGGAGCCTCG GCGCCGGAAG 
TTTCACCGTC CCATCGGCCT AAGAATCCCA CTGCCACCCT CATGGAGAGA GAGCCCGCGG 
GACGCCGGAG AGGGAGACAC CACCAGCCTG CGCCTGCTCT GCAGCGTCAT CGGTGGAACA 
GCTCCTGCCC AGTGGGAAGA TATAACTGGC ACCACCAAAC TGGCGTATTC CAATGACTGT 
GCCAGTTTCA CCACCAACGT CTCTGCCCGA TTCTGGCTGG CGGACTGTCC TCGGACTGCG 
GAGGCCGTGT CCTTTGCCAA CCTCCTGTAC CGCGAGCTGT CGGCCGTGCC GTACATGGCC 
AAGTTTGTAG TGTTTGCGAA GATGAATGAA GCTCGCGAGG GTCGACTCCG CTGCTACTGC 
ATGACGGATG ACAAGATGGA CAAAACCCTG GAGCAGCACG AGAACTTCTC GGAAGTGGCT 
CGCAGCAGAG ACATCGAGGT GATGGAGGGA ATGCCGCTGT ATTTGGAGTG TTCAGGAAAC 
TTGGTTCCTA TAAGAAAAGC AGCCCAGCAG CCTCGCTGTT TCAGCTTCCA GGCCTTTAAA 
GACAACAGAC TGCCTGTGTC CGTCAAGGTG AGGGACAGCA GTAAAGACCC CTCTGGGTTT 
TTGTCTTTCT TGCGCAAGTC AACCAAATAT GAAGACAGCC AACACGTGCT GTGCAACCTC 
AACGTGACCA TGCCGCCGTG CATTAAGATT ATGGGCAGCG ATGAGCGGAG GAGGACTCTG 
ACTCCTCTGG CTCTCAGAGA AAGATACAGC GCTCTGAATG AACCGGCAAT GGCATCACTG 
AGCGCCATGG AAAGGACGGA GTTAAAGATG GCTTTAATAG CTGAACAGCT AGGCCTGAGC 
TGGGCTGAGT TGGGACGGGA ACTGCAGTTT AATGTAGATG AAATAAATAA GATCCGTGTG 
GAGAATCCCA ACTCCCTTTT AGAGCAGAGC TCAACGCTGC TCAACCTGTG GGCCGCCCGA 
GAGGGGAAGA GAGCCAAAAT GGACAGCCTG TACGTGGCCC TCAAGAGCAT CGACCGAGTG 
GACATTGTGA ACATGATGGA GGGCCAGGGA GGACCACCGG CGGGTCAGCA GGGGGGCTGG 
GAGCAGGACG CTTCTAGGTC TAGGCATCAC GACCGAGACC ATCTCTCACC TGGAATCACA 
AATGGTTACG GGCTGGTGGT GCAGGAGGAA CTAGTGTCAC CGGCCTCCAT GCAGTACAGT 
CTGCCCTCGC CACTGGGCAA TGAGCCCTAC TGGCAGGAGG TTTCCAGCAT GGAGTGCGCG 
CCCATCGCCA CCACTGAAGA AGACACGCTC ATGGAGATGT CCGAGGTACA GGTTTGGCCC 
TCAGGAAATA GTCCGTCCAT TGTGGCTGTA GAGGACTCCT CATTGGAGTG CAGCAATGCG 
GACGACTCCG AGGCGCTGCA GAACCGGAGC CTGGGTCACT CCAGGAGCGG GACGCCCGGT 
GGGAACGGAC TCACTCCGGG AGGTTTAAGT GGCTCCATCG AGCTCCTGGA GGATCAGTCC 
ATCGGTGCGG ATTCAGACTA CAGTGCCGCC GGGATGCTGC GTCTGAGCGA AGGGGTCAAC 
ATCAGTGCTC TGGACAGAGG TCAGAGGTCG GAGAGTTCAG AAGGGGCTGT AGGTCACCAA 
GTCACCAACA ACATTAAAGA CGGTTCCAAT GGAGGTTTGG GAGAAGGAGG AGGTGACGGC 
ACACAGTTAG AAGATAGCAT ATCTCTCATT TCAGGGCAAC AGCGTGTGTA TGCTCGAATG 
AGCGAGTCGC CTGGGCTGAG AAGACGGGCA GATCCTGGCG AAGACAGGTT AAGCGCAGGG 
TCGTTCCTGT CTTATCTGCA GGATCAGGGC TCTCCTGGTT GGCATTCGGC TGCAGAAGCG 
ACTCAGAACA GATTGGCGTC TTTGCAATGC TCCCTTCCTA GGCAGGCAAT AGAGTCTATG 
ATGTCATCGG TGCGAGCTAC GGTGGACTCT GACTCCAAGC AGTCTTGTTT GGGCCAGGAG 
GCTCTGATTG AGCCAGTGCG GGACATGGGG CACTCTGAGA TCATCCATGG CCATTATCAG 
GGGACTCAGC CGTTTGAGAA AGGACTCGGC TTCCCGCACA GAACAACCAA CATGCGCACT 
TGGGAGGACA TGCGTCTGAG CGGACAGGGT GATGAGGCTG AGGATCTTCC AGGAGAGCAA 
GTGAGCGAGG AGCAATTCAC AGACGAGCAT GGCAACATCG TCACCAAAAA GATTGTGAGG 
AAAGTGGTCC GGAGAGGAAA AGGGGAGGAA GGGGGTCAGG AGCTGATCAT TGAAGGTCTG 
CAGCAGGATA TCAGTGAGCC TGACTTTGAT GGAGAACAGT TCATGAGCTA TGCCGTCCTG 
GGACGTGACA GCAAGGACTC GACTCCTTCA CCCAAACCAT CCTTCACTGA CTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_009302011.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009300286.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X5, mRNA.

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CloneID	ODa41283
Clone ID Related Accession (Same CDS sequence)	XM_009302011.2
Accession Version	XM_009302011.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5844bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ankyrin 1, erythrocytic a isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302011.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAACCA ACACCATCCT TTTTGGAGCT GACGCTGCCA CCAGTTTCCT CCGCGCAGCT 
CGCTCAGGTA ATCTGGAAAA GGCTCTAGAC CACATCAAGA ACGGCATCGA CATCAACACA 
GCCAATCAGA ATGGACTCAA CGGGCTGCAT TTGGCCTCCA AGGAAGGCCA TGTTAAAATG 
GTGCTGGAGC TGCTTCACCA TGGTATTGTA CTTGAGACGA CAACGAAGAA AGGGAACACT 
GCTCTTCATA TAGCGGCGTT GGCTGGCCAG GAGCAGGTGG TCACTGAACT GGTCAACTAT 
GGAGCAAACG TCAACGCTCA GTCTCAGAAG GGCTTCACGC CGCTCTACAT GGCAGCACAA 
GAGAATCATC TGGAGGTTGT GAAGTTTCTG CTGGATAACG GAGCCAATCA GACCATTCCA 
ACTGAGGATG GCTTTACCCC TCTTGCTGTG GCTCTTCAGC AGGGCCATGA GAATGTGGTG 
GCCCTGCTCA TCAACCACGG TACTAAGGGC AAGGTCCGCC TTCCCGCACT GCACATCGCT 
GCCCGAAACG ACGACACCCG CACTGCCGCT GTGCTCTTAC AGAACGACCC CAATCCTGAC 
GTCCTGAGCA AGACAGGCTT CACGCCGCTC CACATCGCAG CACATTATGA AAACCTGAAT 
GTTGCTCAGC TTCTTCTGAA CCGAGGAGCT GATGTGAACT TCACACCTAA GAACGGGATC 
ACACCGCTGC ACATCGCATC CAGGAGAGGG AACGTCATCA TGGTCAGACT TCTGCTGGAC 
CGAGGAGCAA AGATCGATGC CAAGACAAAA GATGAGTTGA CTCCTCTTCA CTGTGCTGCC 
AGAAATGGCC ATGTGAGAAT CATCGAGATC CTTCTGGACC AAGGAGCCCC AATACAGGCC 
AAGACCAAAA ATGGCCTGTC GCCGATCCAC ATGGCAGCCC AGGGCGATCA CTTGGACTGT 
ATCCGACAGC TCTTACAGTA CAACGCAGAG ATCGATGACA TCACTCTGGA CCACCTGACA 
CCTCTGCATG TGGCGGCTCA CTGCGGGCAT CACCGCGTGG CCAAGGTGCT CCTGGATAAA 
GGAGCCAAAC CAAACACCCG CGCTCTGAAT GGATTCACGC CGCTCCACAT TGCCTGTAAG 
AAGAACCACA TGCGTGTAAT GGACCTGCTA TTAAAGCATT CTGCATCCCT GGAAGCTGTA 
ACCGAGTCAG GCTTGACCCC GCTGCATGTG TCCTCCTTCA TGGGTCATCT GAATATAGTC 
AAGATACTGA TGCAAAAAGG AGCTTCGCCG AACGCCTCAA ATGTGAAAGT GGAAACCCCT 
CTGCATATGG CATCTCGAGC TGGACACTGT GAAGTTGCTG AATTCCTATT GCAAAATGCA 
GCTCCAGTTG ATGCCAAAGC CAAGGATGAC CAGACGCCGC TACACTGTGC CTCCCGCATG 
GGCCACAACG AGATGGTGAA GTTGCTTCTG GAGCACAAGG CCAACTCAAA CTCCACCACC 
ACAGCAGGAC ACACACCGCT GCACATCGCG GCCCGCGAGG GACACACACA AACTGCCAGC 
ATCCTCCTGG ACATGAACGC CCAGCTCACC AAAATGACCA AGAAAGGCTT CACTCCTCTT 
CATGTTGCAG CCAAATACGG AAAGGTGGAC GTGGCAGTTC TGCTTCTGGA GAGGGGAGCA 
AATCCAAACG CTGCTGGAAA AGTTGGACTC ACGCCGCTGC ATGTGGCCGT CCATCATAAC 
AATCTAGATG TGGTCAATCT TTTACTCAGC AAAGGAGGAT CACCACACAG CGCCGCTCGG 
AATGGCTATA CTCCGCTCCA CATTGCTTCC AAACAGAACC AGGCGGAGGT GGCGAGCAGT 
TTACTGCAGC ACGGGGCGTC TGCCAATGCT GAGTCTCTGC AAGGGGTCAC ACCTCTACAC 
CTCGCCTCTC AAGAGGGTCA ACCTGACATG GTGTTACTGC TCATCTCCAA GCAGGCCAAT 
GTAAACCTTG GCAATAAGAG CGGTCTGACT CCTCTTCATC TTGTGGCTCA GGAAGGGCAT 
GTTGGGATTG CTGATATACT AGTGAAACAT GGAGCATCTG TTTATGCTGC CACACGGATG 
GGTTACACTC CGCTTCACGT GGCTTGTCAT TATGGGAATA TCAAGATGGT GAAGTTTCTC 
TTACAGCAGC AGGCCAACGT AAACTGCAAG ACCAGGATGA GCTACACGCC GCTACATCAG 
GCTGCTCAGC AGGGTCATAC GGACATCGTG ACGCTTCTGC TGAAACACGG AGCACAACCG 
AATGAAGTTA CATCTAACGG CACCTCTGCT CTTGCCATCG CCAAGCGGCT TGGATACATT 
TCAGTCATCG ATGTGCTCAA ACTGGTCACT GAAGAGACGG TCACTATGAC CACCACTGAA 
AAACATCGCA TGAGTTTCCC TGAAACCGTT GATGAGATCC TGGATGTGTC TGAAGATGAA 
GGAATTGCAC AGCTAACATT AGGCGAGGAG CTGCTGGGAA ACGAGGGCGC CAGGTATTTG 
ATGAAGATGG AGGACATAAA GGACCATGAT GAGGATTTCC TTTCCCCCAA GAAAGTTCTA 
GAGTACGAGG GAGAGTTGGA GAATCATTCT CCTGCTATCC CGAGGATCCC GTGTGTGTCT 
CCTGAGACTG TCATGCTGAA AGAGCATGAG ATGGAGCAGC AGCACACTCC ACTTCCATTG 
CCCAAAGAGT ACGACGAGGA TTCTCTGATT CCCAGCAGTC CTGCAACTGA GACTTCAGAC 
AATGTCAGTC CGGTTGCCAG TCCCATCCAC ACCGGTTTTC TGGTGAGCTT CATGGTGGAT 
GCTCGCGGCG GCTCGATGCG CGGCAGCAGA CACAACGGCC TCCGCGTCAT CATTCCTCCT 
CGAACCTGCG CCGCTCCCAC ACGCATCACC TGCCGCCTGG TGAAGCCCCA GAAGCTGCCC 
ACACCGCCGC CATTAGTGGA GGGAGAGGGT CTCGCCAGCC GCATCATCTC CCTTGGACCC 
GCCAGCATGC AGTTTTTAGG GCCTGTGATT GTGGAGATTC CTCATTTTGC GGCTCTCGGT 
CGGGGAGATC GGGAACTCGT GGTCTTGAGG AGTGAGAACG GATCGGTCTG GAAGGAACAT 
CGTAACCGAT ATGGAGATGA AGTGCTAGAG ACCATCCTCA ACGGCATGGA TGAAGATCTG 
GAGAGTCAGG AGGAGCTCGG GAAGAAACGC ATCAGACGGA TCATCTCCAC CGACTTCCCG 
CTCTACTTTG CGGTGGTGTC TCGCATCCAG CAGGAGAACG ATCTGATTGG CCCAGAAGGA 
GGTTTTCTGT CCAGTAAACT GGTGCCGATG GTCCAGGCCA CCTTTCCAGA GACAGCCGTC 
ACCAAACGCG TGCGTCTGGG ACTGCAGGCC CAGCCGGTGC CTGATGAGCT GGTGGCCAAG 
CTTTTGGGGA ACCAGGCCAC GTTCAGTCCT GTGGTGACGG TGGAGCCTCG GCGCCGGAAG 
TTTCACCGTC CCATCGGCCT AAGAATCCCA CTGCCACCCT CATGGAGAGA GAGCCCGCGG 
GACGCCGGAG AGGGAGACAC CACCAGCCTG CGCCTGCTCT GCAGCGTCAT CGGTGGAACA 
GCTCCTGCCC AGTGGGAAGA TATAACTGGC ACCACCAAAC TGGCGTATTC CAATGACTGT 
GCCAGTTTCA CCACCAACGT CTCTGCCCGA TTCTGGCTGG CGGACTGTCC TCGGACTGCG 
GAGGCCGTGT CCTTTGCCAA CCTCCTGTAC CGCGAGCTGT CGGCCGTGCC GTACATGGCC 
AAGTTTGTAG TGTTTGCGAA GATGAATGAA GCTCGCGAGG GTCGACTCCG CTGCTACTGC 
ATGACGGATG ACAAGATGGA CAAAACCCTG GAGCAGCACG AGAACTTCTC GGAAGTGGCT 
CGCAGCAGAG ACATCGAGGT GATGGAGGGA ATGCCGCTGT ATTTGGAGTG TTCAGGAAAC 
TTGGTTCCTA TAAGAAAAGC AGCCCAGCAG CCTCGCTGTT TCAGCTTCCA GGCCTTTAAA 
GACAACAGAC TGCCTGTGTC CGTCAAGGTG AGGGACAGCA GTAAAGACCC CTCTGGGTTT 
TTGTCTTTCT TGCGCAAGTC AACCAAATAT GAAGACAGCC AACACGTGCT GTGCAACCTC 
AACGTGACCA TGCCGCCGTG CATTAAGATT ATGGGCAGCG ATGAGCGGAG GAGGACTCTG 
ACTCCTCTGG CTCTCAGAGA AAGATACAGC GCTCTGAATG AACCGGCAAT GGCATCACTG 
AGCGCCATGG AAAGGACGGA GTTAAAGATG GCTTTAATAG CTGAACAGCT AGGCCTGAGC 
TGGGCTGAGT TGGGACGGGA ACTGCAGTTT AATGTAGATG AAATAAATAA GATCCGTGTG 
GAGAATCCCA ACTCCCTTTT AGAGCAGAGC TCAACGCTGC TCAACCTGTG GGCCGCCCGA 
GAGGGGAAGA GAGCCAAAAT GGACAGCCTG TACGTGGCCC TCAAGAGCAT CGACCGAGTG 
GACATTGTGA ACATGATGGA GGGCCAGGGA GGACCACCGG CGGGTCAGCA GGGGGGCTGG 
GAGCAGGACG CTTCTAGGTC TAGGCATCAC GACCGAGACC ATCTCTCACC TGGAATCACA 
AATGGTTACG GGCTGGTGGT GCAGGAGGAA CTAGTGTCAC CGGCCTCCAT GCAGTACAGT 
CTGCCCTCGC CACTGGGCAA TGAGCCCTAC TGGCAGGAGG TTTCCAGCAT GGAGTGCGCG 
CCCATCGCCA CCACTGAAGA AGACACGCTC ATGGAGATGT CCGAGGTACA GGTTTGGCCC 
TCAGGAAATA GTCCGTCCAT TGTGGCTGTA GAGGACTCCT CATTGGAGTG CAGCAATGCG 
GACGACTCCG AGGCGCTGCA GAACCGGAGC CTGGGTCACT CCAGGAGCGG GACGCCCGGT 
GGGAACGGAC TCACTCCGGG AGGTTTAAGT GGCTCCATCG AGCTCCTGGA GGATCAGTCC 
ATCGGTGCGG ATTCAGACTA CAGTGCCGCC GGGATGCTGC GTCTGAGCGA AGGGGTCAAC 
ATCAGTGCTC TGGACAGAGG TCAGAGGTCG GAGAGTTCAG AAGGGGCTGT AGGTCACCAA 
GTCACCAACA ACATTAAAGA CGGTTCCAAT GGAGGTTTGG GAGAAGGAGG AGGTGACGGC 
ACACAGTTAG AAGATAGCAT ATCTCTCATT TCAGGGCAAC AGCGTGTGTA TGCTCGAATG 
AGCGAGTCGC CTGGGCTGAG AAGACGGGCA GATCCTGGCG AAGACAGGTT AAGCGCAGGG 
TCGTTCCTGT CTTATCTGCA GGATCAGGGC TCTCCTGGTT GGCATTCGGC TGCAGAAGCG 
ACTCAGAACA GATTGGCGTC TTTGCAATGC TCCCTTCCTA GGCAGGCAAT AGAGTCTATG 
ATGTCATCGG TGCGAGCTAC GGTGGACTCT GACTCCAAGC AGTCTTGTTT GGGCCAGGAG 
GCTCTGATTG AGCCAGTGCG GGACATGGGG CACTCTGAGA TCATCCATGG CCATTATCAG 
GGGACTCAGC CGTTTGAGAA AGGACTCGGC TTCCCGCACA GAACAACCAA CATGCGCACT 
TGGGAGGACA TGCGTCTGAG CGGACAGGGT GATGAGGCTG AGGATCTTCC AGGAGAGCAA 
GTGAGCGAGG AGCAATTCAC AGACGAGCAT GGCAACATCG TCACCAAAAA GATTGTGAGG 
AAAGTGGTCC GGAGAGGAAA AGGGGAGGAA GGGGGTCAGG AGCTGATCAT TGAAGGTCTG 
CAGCAGGATA TCAGTGAGCC TGACTTTGAT GGAGAACAGT TCATGAGCTA TGCCGTCCTG 
GGACGTGACA GCAAGATCTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300286.1
CDS	343..6186
Translation	MPTNTILFGADAATSFLRAARSGNLEKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLINHGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGLTPLHVSSFMGHLNIVKILMQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGANPNAAGKVGLTPLHVAVHHNNLDVVNLLLSKGGSPHSAARNGYTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPLHLASQEGQPDMVLLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKHGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSNGTSALAIAKRLGYISVIDVLKLVTEETVTMTTTEKHRMSFPETVDEILDVSEDEGIAQLTLGEELLGNEGARYLMKMEDIKDHDEDFLSPKKVLEYEGELENHSPAIPRIPCVSPETVMLKEHEMEQQHTPLPLPKEYDEDSLIPSSPATETSDNVSPVASPIHTGFLVSFMVDARGGSMRGSRHNGLRVIIPPRTCAAPTRITCRLVKPQKLPTPPPLVEGEGLASRIISLGPASMQFLGPVIVEIPHFAALGRGDRELVVLRSENGSVWKEHRNRYGDEVLETILNGMDEDLESQEELGKKRIRRIISTDFPLYFAVVSRIQQENDLIGPEGGFLSSKLVPMVQATFPETAVTKRVRLGLQAQPVPDELVAKLLGNQATFSPVVTVEPRRRKFHRPIGLRIPLPPSWRESPRDAGEGDTTSLRLLCSVIGGTAPAQWEDITGTTKLAYSNDCASFTTNVSARFWLADCPRTAEAVSFANLLYRELSAVPYMAKFVVFAKMNEAREGRLRCYCMTDDKMDKTLEQHENFSEVARSRDIEVMEGMPLYLECSGNLVPIRKAAQQPRCFSFQAFKDNRLPVSVKVRDSSKDPSGFLSFLRKSTKYEDSQHVLCNLNVTMPPCIKIMGSDERRRTLTPLALRERYSALNEPAMASLSAMERTELKMALIAEQLGLSWAELGRELQFNVDEINKIRVENPNSLLEQSSTLLNLWAAREGKRAKMDSLYVALKSIDRVDIVNMMEGQGGPPAGQQGGWEQDASRSRHHDRDHLSPGITNGYGLVVQEELVSPASMQYSLPSPLGNEPYWQEVSSMECAPIATTEEDTLMEMSEVQVWPSGNSPSIVAVEDSSLECSNADDSEALQNRSLGHSRSGTPGGNGLTPGGLSGSIELLEDQSIGADSDYSAAGMLRLSEGVNISALDRGQRSESSEGAVGHQVTNNIKDGSNGGLGEGGGDGTQLEDSISLISGQQRVYARMSESPGLRRRADPGEDRLSAGSFLSYLQDQGSPGWHSAAEATQNRLASLQCSLPRQAIESMMSSVRATVDSDSKQSCLGQEALIEPVRDMGHSEIIHGHYQGTQPFEKGLGFPHRTTNMRTWEDMRLSGQGDEAEDLPGEQVSEEQFTDEHGNIVTKKIVRKVVRRGKGEEGGQELIIEGLQQDISEPDFDGEQFMSYAVLGRDSKIL

Target ORF information:

RefSeq Version	XM_009302011.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302011.2

ORF Insert Sequence:

ATGCCAACCA ACACCATCCT TTTTGGAGCT GACGCTGCCA CCAGTTTCCT CCGCGCAGCT 
CGCTCAGGTA ATCTGGAAAA GGCTCTAGAC CACATCAAGA ACGGCATCGA CATCAACACA 
GCCAATCAGA ATGGACTCAA CGGGCTGCAT TTGGCCTCCA AGGAAGGCCA TGTTAAAATG 
GTGCTGGAGC TGCTTCACCA TGGTATTGTA CTTGAGACGA CAACGAAGAA AGGGAACACT 
GCTCTTCATA TAGCGGCGTT GGCTGGCCAG GAGCAGGTGG TCACTGAACT GGTCAACTAT 
GGAGCAAACG TCAACGCTCA GTCTCAGAAG GGCTTCACGC CGCTCTACAT GGCAGCACAA 
GAGAATCATC TGGAGGTTGT GAAGTTTCTG CTGGATAACG GAGCCAATCA GACCATTCCA 
ACTGAGGATG GCTTTACCCC TCTTGCTGTG GCTCTTCAGC AGGGCCATGA GAATGTGGTG 
GCCCTGCTCA TCAACCACGG TACTAAGGGC AAGGTCCGCC TTCCCGCACT GCACATCGCT 
GCCCGAAACG ACGACACCCG CACTGCCGCT GTGCTCTTAC AGAACGACCC CAATCCTGAC 
GTCCTGAGCA AGACAGGCTT CACGCCGCTC CACATCGCAG CACATTATGA AAACCTGAAT 
GTTGCTCAGC TTCTTCTGAA CCGAGGAGCT GATGTGAACT TCACACCTAA GAACGGGATC 
ACACCGCTGC ACATCGCATC CAGGAGAGGG AACGTCATCA TGGTCAGACT TCTGCTGGAC 
CGAGGAGCAA AGATCGATGC CAAGACAAAA GATGAGTTGA CTCCTCTTCA CTGTGCTGCC 
AGAAATGGCC ATGTGAGAAT CATCGAGATC CTTCTGGACC AAGGAGCCCC AATACAGGCC 
AAGACCAAAA ATGGCCTGTC GCCGATCCAC ATGGCAGCCC AGGGCGATCA CTTGGACTGT 
ATCCGACAGC TCTTACAGTA CAACGCAGAG ATCGATGACA TCACTCTGGA CCACCTGACA 
CCTCTGCATG TGGCGGCTCA CTGCGGGCAT CACCGCGTGG CCAAGGTGCT CCTGGATAAA 
GGAGCCAAAC CAAACACCCG CGCTCTGAAT GGATTCACGC CGCTCCACAT TGCCTGTAAG 
AAGAACCACA TGCGTGTAAT GGACCTGCTA TTAAAGCATT CTGCATCCCT GGAAGCTGTA 
ACCGAGTCAG GCTTGACCCC GCTGCATGTG TCCTCCTTCA TGGGTCATCT GAATATAGTC 
AAGATACTGA TGCAAAAAGG AGCTTCGCCG AACGCCTCAA ATGTGAAAGT GGAAACCCCT 
CTGCATATGG CATCTCGAGC TGGACACTGT GAAGTTGCTG AATTCCTATT GCAAAATGCA 
GCTCCAGTTG ATGCCAAAGC CAAGGATGAC CAGACGCCGC TACACTGTGC CTCCCGCATG 
GGCCACAACG AGATGGTGAA GTTGCTTCTG GAGCACAAGG CCAACTCAAA CTCCACCACC 
ACAGCAGGAC ACACACCGCT GCACATCGCG GCCCGCGAGG GACACACACA AACTGCCAGC 
ATCCTCCTGG ACATGAACGC CCAGCTCACC AAAATGACCA AGAAAGGCTT CACTCCTCTT 
CATGTTGCAG CCAAATACGG AAAGGTGGAC GTGGCAGTTC TGCTTCTGGA GAGGGGAGCA 
AATCCAAACG CTGCTGGAAA AGTTGGACTC ACGCCGCTGC ATGTGGCCGT CCATCATAAC 
AATCTAGATG TGGTCAATCT TTTACTCAGC AAAGGAGGAT CACCACACAG CGCCGCTCGG 
AATGGCTATA CTCCGCTCCA CATTGCTTCC AAACAGAACC AGGCGGAGGT GGCGAGCAGT 
TTACTGCAGC ACGGGGCGTC TGCCAATGCT GAGTCTCTGC AAGGGGTCAC ACCTCTACAC 
CTCGCCTCTC AAGAGGGTCA ACCTGACATG GTGTTACTGC TCATCTCCAA GCAGGCCAAT 
GTAAACCTTG GCAATAAGAG CGGTCTGACT CCTCTTCATC TTGTGGCTCA GGAAGGGCAT 
GTTGGGATTG CTGATATACT AGTGAAACAT GGAGCATCTG TTTATGCTGC CACACGGATG 
GGTTACACTC CGCTTCACGT GGCTTGTCAT TATGGGAATA TCAAGATGGT GAAGTTTCTC 
TTACAGCAGC AGGCCAACGT AAACTGCAAG ACCAGGATGA GCTACACGCC GCTACATCAG 
GCTGCTCAGC AGGGTCATAC GGACATCGTG ACGCTTCTGC TGAAACACGG AGCACAACCG 
AATGAAGTTA CATCTAACGG CACCTCTGCT CTTGCCATCG CCAAGCGGCT TGGATACATT 
TCAGTCATCG ATGTGCTCAA ACTGGTCACT GAAGAGACGG TCACTATGAC CACCACTGAA 
AAACATCGCA TGAGTTTCCC TGAAACCGTT GATGAGATCC TGGATGTGTC TGAAGATGAA 
GGAATTGCAC AGCTAACATT AGGCGAGGAG CTGCTGGGAA ACGAGGGCGC CAGGTATTTG 
ATGAAGATGG AGGACATAAA GGACCATGAT GAGGATTTCC TTTCCCCCAA GAAAGTTCTA 
GAGTACGAGG GAGAGTTGGA GAATCATTCT CCTGCTATCC CGAGGATCCC GTGTGTGTCT 
CCTGAGACTG TCATGCTGAA AGAGCATGAG ATGGAGCAGC AGCACACTCC ACTTCCATTG 
CCCAAAGAGT ACGACGAGGA TTCTCTGATT CCCAGCAGTC CTGCAACTGA GACTTCAGAC 
AATGTCAGTC CGGTTGCCAG TCCCATCCAC ACCGGTTTTC TGGTGAGCTT CATGGTGGAT 
GCTCGCGGCG GCTCGATGCG CGGCAGCAGA CACAACGGCC TCCGCGTCAT CATTCCTCCT 
CGAACCTGCG CCGCTCCCAC ACGCATCACC TGCCGCCTGG TGAAGCCCCA GAAGCTGCCC 
ACACCGCCGC CATTAGTGGA GGGAGAGGGT CTCGCCAGCC GCATCATCTC CCTTGGACCC 
GCCAGCATGC AGTTTTTAGG GCCTGTGATT GTGGAGATTC CTCATTTTGC GGCTCTCGGT 
CGGGGAGATC GGGAACTCGT GGTCTTGAGG AGTGAGAACG GATCGGTCTG GAAGGAACAT 
CGTAACCGAT ATGGAGATGA AGTGCTAGAG ACCATCCTCA ACGGCATGGA TGAAGATCTG 
GAGAGTCAGG AGGAGCTCGG GAAGAAACGC ATCAGACGGA TCATCTCCAC CGACTTCCCG 
CTCTACTTTG CGGTGGTGTC TCGCATCCAG CAGGAGAACG ATCTGATTGG CCCAGAAGGA 
GGTTTTCTGT CCAGTAAACT GGTGCCGATG GTCCAGGCCA CCTTTCCAGA GACAGCCGTC 
ACCAAACGCG TGCGTCTGGG ACTGCAGGCC CAGCCGGTGC CTGATGAGCT GGTGGCCAAG 
CTTTTGGGGA ACCAGGCCAC GTTCAGTCCT GTGGTGACGG TGGAGCCTCG GCGCCGGAAG 
TTTCACCGTC CCATCGGCCT AAGAATCCCA CTGCCACCCT CATGGAGAGA GAGCCCGCGG 
GACGCCGGAG AGGGAGACAC CACCAGCCTG CGCCTGCTCT GCAGCGTCAT CGGTGGAACA 
GCTCCTGCCC AGTGGGAAGA TATAACTGGC ACCACCAAAC TGGCGTATTC CAATGACTGT 
GCCAGTTTCA CCACCAACGT CTCTGCCCGA TTCTGGCTGG CGGACTGTCC TCGGACTGCG 
GAGGCCGTGT CCTTTGCCAA CCTCCTGTAC CGCGAGCTGT CGGCCGTGCC GTACATGGCC 
AAGTTTGTAG TGTTTGCGAA GATGAATGAA GCTCGCGAGG GTCGACTCCG CTGCTACTGC 
ATGACGGATG ACAAGATGGA CAAAACCCTG GAGCAGCACG AGAACTTCTC GGAAGTGGCT 
CGCAGCAGAG ACATCGAGGT GATGGAGGGA ATGCCGCTGT ATTTGGAGTG TTCAGGAAAC 
TTGGTTCCTA TAAGAAAAGC AGCCCAGCAG CCTCGCTGTT TCAGCTTCCA GGCCTTTAAA 
GACAACAGAC TGCCTGTGTC CGTCAAGGTG AGGGACAGCA GTAAAGACCC CTCTGGGTTT 
TTGTCTTTCT TGCGCAAGTC AACCAAATAT GAAGACAGCC AACACGTGCT GTGCAACCTC 
AACGTGACCA TGCCGCCGTG CATTAAGATT ATGGGCAGCG ATGAGCGGAG GAGGACTCTG 
ACTCCTCTGG CTCTCAGAGA AAGATACAGC GCTCTGAATG AACCGGCAAT GGCATCACTG 
AGCGCCATGG AAAGGACGGA GTTAAAGATG GCTTTAATAG CTGAACAGCT AGGCCTGAGC 
TGGGCTGAGT TGGGACGGGA ACTGCAGTTT AATGTAGATG AAATAAATAA GATCCGTGTG 
GAGAATCCCA ACTCCCTTTT AGAGCAGAGC TCAACGCTGC TCAACCTGTG GGCCGCCCGA 
GAGGGGAAGA GAGCCAAAAT GGACAGCCTG TACGTGGCCC TCAAGAGCAT CGACCGAGTG 
GACATTGTGA ACATGATGGA GGGCCAGGGA GGACCACCGG CGGGTCAGCA GGGGGGCTGG 
GAGCAGGACG CTTCTAGGTC TAGGCATCAC GACCGAGACC ATCTCTCACC TGGAATCACA 
AATGGTTACG GGCTGGTGGT GCAGGAGGAA CTAGTGTCAC CGGCCTCCAT GCAGTACAGT 
CTGCCCTCGC CACTGGGCAA TGAGCCCTAC TGGCAGGAGG TTTCCAGCAT GGAGTGCGCG 
CCCATCGCCA CCACTGAAGA AGACACGCTC ATGGAGATGT CCGAGGTACA GGTTTGGCCC 
TCAGGAAATA GTCCGTCCAT TGTGGCTGTA GAGGACTCCT CATTGGAGTG CAGCAATGCG 
GACGACTCCG AGGCGCTGCA GAACCGGAGC CTGGGTCACT CCAGGAGCGG GACGCCCGGT 
GGGAACGGAC TCACTCCGGG AGGTTTAAGT GGCTCCATCG AGCTCCTGGA GGATCAGTCC 
ATCGGTGCGG ATTCAGACTA CAGTGCCGCC GGGATGCTGC GTCTGAGCGA AGGGGTCAAC 
ATCAGTGCTC TGGACAGAGG TCAGAGGTCG GAGAGTTCAG AAGGGGCTGT AGGTCACCAA 
GTCACCAACA ACATTAAAGA CGGTTCCAAT GGAGGTTTGG GAGAAGGAGG AGGTGACGGC 
ACACAGTTAG AAGATAGCAT ATCTCTCATT TCAGGGCAAC AGCGTGTGTA TGCTCGAATG 
AGCGAGTCGC CTGGGCTGAG AAGACGGGCA GATCCTGGCG AAGACAGGTT AAGCGCAGGG 
TCGTTCCTGT CTTATCTGCA GGATCAGGGC TCTCCTGGTT GGCATTCGGC TGCAGAAGCG 
ACTCAGAACA GATTGGCGTC TTTGCAATGC TCCCTTCCTA GGCAGGCAAT AGAGTCTATG 
ATGTCATCGG TGCGAGCTAC GGTGGACTCT GACTCCAAGC AGTCTTGTTT GGGCCAGGAG 
GCTCTGATTG AGCCAGTGCG GGACATGGGG CACTCTGAGA TCATCCATGG CCATTATCAG 
GGGACTCAGC CGTTTGAGAA AGGACTCGGC TTCCCGCACA GAACAACCAA CATGCGCACT 
TGGGAGGACA TGCGTCTGAG CGGACAGGGT GATGAGGCTG AGGATCTTCC AGGAGAGCAA 
GTGAGCGAGG AGCAATTCAC AGACGAGCAT GGCAACATCG TCACCAAAAA GATTGTGAGG 
AAAGTGGTCC GGAGAGGAAA AGGGGAGGAA GGGGGTCAGG AGCTGATCAT TGAAGGTCTG 
CAGCAGGATA TCAGTGAGCC TGACTTTGAT GGAGAACAGT TCATGAGCTA TGCCGTCCTG 
GGACGTGACA GCAAGATCTT GTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_009302013.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009300288.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X6, mRNA.

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CloneID	ODa41285
Clone ID Related Accession (Same CDS sequence)	XM_009302013.2
Accession Version	XM_009302013.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5823bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ankyrin 1, erythrocytic a isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302013.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAACCA ACACCATCCT TTTTGGAGCT GACGCTGCCA CCAGTTTCCT CCGCGCAGCT 
CGCTCAGGTA ATCTGGAAAA GGCTCTAGAC CACATCAAGA ACGGCATCGA CATCAACACA 
GCCAATCAGA ATGGACTCAA CGGGCTGCAT TTGGCCTCCA AGGAAGGCCA TGTTAAAATG 
GTGCTGGAGC TGCTTCACCA TGGTATTGTA CTTGAGACGA CAACGAAGAA AGGGAACACT 
GCTCTTCATA TAGCGGCGTT GGCTGGCCAG GAGCAGGTGG TCACTGAACT GGTCAACTAT 
GGAGCAAACG TCAACGCTCA GTCTCAGAAG GGCTTCACGC CGCTCTACAT GGCAGCACAA 
GAGAATCATC TGGAGGTTGT GAAGTTTCTG CTGGATAACG GAGCCAATCA GACCATTCCA 
ACTGAGGATG GCTTTACCCC TCTTGCTGTG GCTCTTCAGC AGGGCCATGA GAATGTGGTG 
GCCCTGCTCA TCAACCACGG TACTAAGGGC AAGGTCCGCC TTCCCGCACT GCACATCGCT 
GCCCGAAACG ACGACACCCG CACTGCCGCT GTGCTCTTAC AGAACGACCC CAATCCTGAC 
GTCCTGAGCA AGACAGGCTT CACGCCGCTC CACATCGCAG CACATTATGA AAACCTGAAT 
GTTGCTCAGC TTCTTCTGAA CCGAGGAGCT GATGTGAACT TCACACCTAA GAACGGGATC 
ACACCGCTGC ACATCGCATC CAGGAGAGGG AACGTCATCA TGGTCAGACT TCTGCTGGAC 
CGAGGAGCAA AGATCGATGC CAAGACAAAA GATGAGTTGA CTCCTCTTCA CTGTGCTGCC 
AGAAATGGCC ATGTGAGAAT CATCGAGATC CTTCTGGACC AAGGAGCCCC AATACAGGCC 
AAGACCAAAA ATGGCCTGTC GCCGATCCAC ATGGCAGCCC AGGGCGATCA CTTGGACTGT 
ATCCGACAGC TCTTACAGTA CAACGCAGAG ATCGATGACA TCACTCTGGA CCACCTGACA 
CCTCTGCATG TGGCGGCTCA CTGCGGGCAT CACCGCGTGG CCAAGGTGCT CCTGGATAAA 
GGAGCCAAAC CAAACACCCG CGCTCTGAAT GGATTCACGC CGCTCCACAT TGCCTGTAAG 
AAGAACCACA TGCGTGTAAT GGACCTGCTA TTAAAGCATT CTGCATCCCT GGAAGCTGTA 
ACCGAGTCAG GCTTGACCCC GCTGCATGTG TCCTCCTTCA TGGGTCATCT GAATATAGTC 
AAGATACTGA TGCAAAAAGG AGCTTCGCCG AACGCCTCAA ATGTGAAAGT GGAAACCCCT 
CTGCATATGG CATCTCGAGC TGGACACTGT GAAGTTGCTG AATTCCTATT GCAAAATGCA 
GCTCCAGTTG ATGCCAAAGC CAAGGATGAC CAGACGCCGC TACACTGTGC CTCCCGCATG 
GGCCACAACG AGATGGTGAA GTTGCTTCTG GAGCACAAGG CCAACTCAAA CTCCACCACC 
ACAGCAGGAC ACACACCGCT GCACATCGCG GCCCGCGAGG GACACACACA AACTGCCAGC 
ATCCTCCTGG ACATGAACGC CCAGCTCACC AAAATGACCA AGAAAGGCTT CACTCCTCTT 
CATGTTGCAG CCAAATACGG AAAGGTGGAC GTGGCAGTTC TGCTTCTGGA GAGGGGAGCA 
AATCCAAACG CTGCTGGAAA AGTTGGACTC ACGCCGCTGC ATGTGGCCGT CCATCATAAC 
AATCTAGATG TGGTCAATCT TTTACTCAGC AAAGGAGGAT CACCACACAG CGCCGCTCGG 
AATGGCTATA CTCCGCTCCA CATTGCTTCC AAACAGAACC AGGCGGAGGT GGCGAGCAGT 
TTACTGCAGC ACGGGGCGTC TGCCAATGCT GAGTCTCTGC AAGGGGTCAC ACCTCTACAC 
CTCGCCTCTC AAGAGGGTCA ACCTGACATG GTGTTACTGC TCATCTCCAA GCAGGCCAAT 
GTAAACCTTG GCAATAAGAG CGGTCTGACT CCTCTTCATC TTGTGGCTCA GGAAGGGCAT 
GTTGGGATTG CTGATATACT AGTGAAACAT GGAGCATCTG TTTATGCTGC CACACGGATG 
GGTTACACTC CGCTTCACGT GGCTTGTCAT TATGGGAATA TCAAGATGGT GAAGTTTCTC 
TTACAGCAGC AGGCCAACGT AAACTGCAAG ACCAGGATGA GCTACACGCC GCTACATCAG 
GCTGCTCAGC AGGGTCATAC GGACATCGTG ACGCTTCTGC TGAAACACGG AGCACAACCG 
AATGAAGTTA CATCTAACGG CACCTCTGCT CTTGCCATCG CCAAGCGGCT TGGATACATT 
TCAGTCATCG ATGTGCTCAA ACTGGTCACT GAAGAGACGG TCACTATGAC CACCACTGAA 
AAACATCGCA TGAGTTTCCC TGAAACCGTT GATGAGATCC TGGATGTGTC TGAAGATGAA 
GGAATTGCAC AGCTAACATT AGGCGAGGAG CTGCTGGGAA ACGAGGGCGC CAGGAATCAT 
TCTCCTGCTA TCCCGAGGAT CCCGTGTGTG TCTCCTGAGA CTGTCATGCT GAAAGAGCAT 
GAGATGGAGC AGCAGCACAC TCCACTTCCA TTGCCCAAAG AGTACGACGA GGATTCTCTG 
ATTCCCAGCA GTCCTGCAAC TGAGACTTCA GACAATGTCA GTCCGGTTGC CAGTCCCATC 
CACACCGGTT TTCTGGTGAG CTTCATGGTG GATGCTCGCG GCGGCTCGAT GCGCGGCAGC 
AGACACAACG GCCTCCGCGT CATCATTCCT CCTCGAACCT GCGCCGCTCC CACACGCATC 
ACCTGCCGCC TGGTGAAGCC CCAGAAGCTG CCCACACCGC CGCCATTAGT GGAGGGAGAG 
GGTCTCGCCA GCCGCATCAT CTCCCTTGGA CCCGCCAGCA TGCAGTTTTT AGGGCCTGTG 
ATTGTGGAGA TTCCTCATTT TGCGGCTCTC GGTCGGGGAG ATCGGGAACT CGTGGTCTTG 
AGGAGTGAGA ACGGATCGGT CTGGAAGGAA CATCGTAACC GATATGGAGA TGAAGTGCTA 
GAGACCATCC TCAACGGCAT GGATGAAGAT CTGGAGAGTC AGGAGGAGCT CGGGAAGAAA 
CGCATCAGAC GGATCATCTC CACCGACTTC CCGCTCTACT TTGCGGTGGT GTCTCGCATC 
CAGCAGGAGA ACGATCTGAT TGGCCCAGAA GGAGGTTTTC TGTCCAGTAA ACTGGTGCCG 
ATGGTCCAGG CCACCTTTCC AGAGACAGCC GTCACCAAAC GCGTGCGTCT GGGACTGCAG 
GCCCAGCCGG TGCCTGATGA GCTGGTGGCC AAGCTTTTGG GGAACCAGGC CACGTTCAGT 
CCTGTGGTGA CGGTGGAGCC TCGGCGCCGG AAGTTTCACC GTCCCATCGG CCTAAGAATC 
CCACTGCCAC CCTCATGGAG AGAGAGCCCG CGGGACGCCG GAGAGGGAGA CACCACCAGC 
CTGCGCCTGC TCTGCAGCGT CATCGGTGGA ACAGCTCCTG CCCAGTGGGA AGATATAACT 
GGCACCACCA AACTGGCGTA TTCCAATGAC TGTGCCAGTT TCACCACCAA CGTCTCTGCC 
CGATTCTGGC TGGCGGACTG TCCTCGGACT GCGGAGGCCG TGTCCTTTGC CAACCTCCTG 
TACCGCGAGC TGTCGGCCGT GCCGTACATG GCCAAGTTTG TAGTGTTTGC GAAGATGAAT 
GAAGCTCGCG AGGGTCGACT CCGCTGCTAC TGCATGACGG ATGACAAGAT GGACAAAACC 
CTGGAGCAGC ACGAGAACTT CTCGGAAGTG GCTCGCAGCA GAGACATCGA GGTGATGGAG 
GGAATGCCGC TGTATTTGGA GTGTTCAGGA AACTTGGTTC CTATAAGAAA AGCAGCCCAG 
CAGCCTCGCT GTTTCAGCTT CCAGGCCTTT AAAGACAACA GACTGCCTGT GTCCGTCAAG 
GTGAGGGACA GCAGTAAAGA CCCCTCTGGG TTTTTGTCTT TCTTGCGCAA GTCAACCAAA 
TATGAAGACA GCCAACACGT GCTGTGCAAC CTCAACGTGA CCATGCCGCC GTGCATTAAG 
ATTATGGGCA GCGATGAGCG GAGGAGGACT CTGACTCCTC TGGCTCTCAG AGAAAGATAC 
AGCGCTCTGA ATGAACCGGC AATGGCATCA CTGAGCGCCA TGGAAAGGAC GGAGTTAAAG 
ATGGCTTTAA TAGCTGAACA GCTAGGCCTG AGCTGGGCTG AGTTGGGACG GGAACTGCAG 
TTTAATGTAG ATGAAATAAA TAAGATCCGT GTGGAGAATC CCAACTCCCT TTTAGAGCAG 
AGCTCAACGC TGCTCAACCT GTGGGCCGCC CGAGAGGGGA AGAGAGCCAA AATGGACAGC 
CTGTACGTGG CCCTCAAGAG CATCGACCGA GTGGACATTG TGAACATGAT GGAGGGCCAG 
GGAGGACCAC CGGCGGGTCA GCAGGGGGGC TGGGAGCAGG ACGCTTCTAG GTCTAGGCAT 
CACGACCGAG ACCATCTCTC ACCTGGAATC ACAAATGGTT ACGGGCTGGT GGTGCAGGAG 
GAACTAGTGT CACCGGCCTC CATGCAGTAC AGTCTGCCCT CGCCACTGGG CAATGAGCCC 
TACTGGCAGG AGGTTTCCAG CATGGAGTGC GCGCCCATCG CCACCACTGA AGAAGACACG 
CTCATGGAGA TGTCCGAGGT ACAGGTTTGG CCCTCAGGAA ATAGTCCGTC CATTGTGGCT 
GTAGAGGACT CCTCATTGGA GTGCAGCAAT GCGGACGACT CCGAGGCGCT GCAGAACCGG 
AGCCTGGGTC ACTCCAGGAG CGGGACGCCC GGTGGGAACG GACTCACTCC GGGAGGTTTA 
AGTGGCTCCA TCGAGCTCCT GGAGGATCAG TCCATCGGTG CGGATTCAGA CTACAGTGCC 
GCCGGGATGC TGCGTCTGAG CGAAGGGGTC AACATCAGTG CTCTGGACAG AGGTCAGAGG 
TCGGAGAGTT CAGAAGGGGC TGTAGGTCAC CAAGTCACCA ACAACATTAA AGACGGTTCC 
AATGGAGGTT TGGGAGAAGG AGGAGGTGAC GGCACACAGT TAGAAGATAG CATATCTCTC 
ATTTCAGGGC AACAGCGTGT GTATGCTCGA ATGAGCGAGT CGCCTGGGCT GAGAAGACGG 
GCAGATCCTG GCGAAGACAG GTTAAGCGCA GGGTCGTTCC TGTCTTATCT GCAGGATCAG 
GGCTCTCCTG GTTGGCATTC GGCTGCAGAA GCGACTCAGA ACAGATTGGC GTCTTTGCAA 
TGCTCCCTTC CTAGGCAGGC AATAGAGTCT ATGATGTCAT CGGTGCGAGC TACGGTGGAC 
TCTGACTCCA AGCAGTCTTG TTTGGGCCAG GAGGCTCTGA TTGAGCCAGT GCGGGACATG 
GGGCACTCTG AGATCATCCA TGGCCATTAT CAGGGGACTC AGCCGTTTGA GAAAGGACTC 
GGCTTCCCGC ACAGAACAAC CAACATGCGC ACTTGGGAGG ACATGCGTCT GAGCGGACAG 
GGTGATGAGG CTGAGGATCT TCCAGGAGAG CAAGTGAGCG AGGAGCAATT CACAGACGAG 
CATGGCAACA TCGTCACCAA AAAGATTGTG AGGAAAGTGG TCCGGAGAGG AAAAGGGGAG 
GAAGGGGGTC AGGAGCTGAT CATTGAAGGT CTGCAGCAGG ATATCAGTGA GCCTGACTTT 
GATGGAGAAC AGTTCATGAG CTATGCCGTC CTGGGACGTG ACAGCAAGCC GGATGTTGTG 
GATGTGAAGA GAGGAGGTGC TCAGATAGTC AAATGTGCGA GTCTGCGGAG AGTAAAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300288.1
CDS	343..6165
Translation	MPTNTILFGADAATSFLRAARSGNLEKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLINHGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGLTPLHVSSFMGHLNIVKILMQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGANPNAAGKVGLTPLHVAVHHNNLDVVNLLLSKGGSPHSAARNGYTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPLHLASQEGQPDMVLLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKHGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSNGTSALAIAKRLGYISVIDVLKLVTEETVTMTTTEKHRMSFPETVDEILDVSEDEGIAQLTLGEELLGNEGARNHSPAIPRIPCVSPETVMLKEHEMEQQHTPLPLPKEYDEDSLIPSSPATETSDNVSPVASPIHTGFLVSFMVDARGGSMRGSRHNGLRVIIPPRTCAAPTRITCRLVKPQKLPTPPPLVEGEGLASRIISLGPASMQFLGPVIVEIPHFAALGRGDRELVVLRSENGSVWKEHRNRYGDEVLETILNGMDEDLESQEELGKKRIRRIISTDFPLYFAVVSRIQQENDLIGPEGGFLSSKLVPMVQATFPETAVTKRVRLGLQAQPVPDELVAKLLGNQATFSPVVTVEPRRRKFHRPIGLRIPLPPSWRESPRDAGEGDTTSLRLLCSVIGGTAPAQWEDITGTTKLAYSNDCASFTTNVSARFWLADCPRTAEAVSFANLLYRELSAVPYMAKFVVFAKMNEAREGRLRCYCMTDDKMDKTLEQHENFSEVARSRDIEVMEGMPLYLECSGNLVPIRKAAQQPRCFSFQAFKDNRLPVSVKVRDSSKDPSGFLSFLRKSTKYEDSQHVLCNLNVTMPPCIKIMGSDERRRTLTPLALRERYSALNEPAMASLSAMERTELKMALIAEQLGLSWAELGRELQFNVDEINKIRVENPNSLLEQSSTLLNLWAAREGKRAKMDSLYVALKSIDRVDIVNMMEGQGGPPAGQQGGWEQDASRSRHHDRDHLSPGITNGYGLVVQEELVSPASMQYSLPSPLGNEPYWQEVSSMECAPIATTEEDTLMEMSEVQVWPSGNSPSIVAVEDSSLECSNADDSEALQNRSLGHSRSGTPGGNGLTPGGLSGSIELLEDQSIGADSDYSAAGMLRLSEGVNISALDRGQRSESSEGAVGHQVTNNIKDGSNGGLGEGGGDGTQLEDSISLISGQQRVYARMSESPGLRRRADPGEDRLSAGSFLSYLQDQGSPGWHSAAEATQNRLASLQCSLPRQAIESMMSSVRATVDSDSKQSCLGQEALIEPVRDMGHSEIIHGHYQGTQPFEKGLGFPHRTTNMRTWEDMRLSGQGDEAEDLPGEQVSEEQFTDEHGNIVTKKIVRKVVRRGKGEEGGQELIIEGLQQDISEPDFDGEQFMSYAVLGRDSKPDVVDVKRGGAQIVKCASLRRVKQ

Target ORF information:

RefSeq Version	XM_009302013.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302013.2

ORF Insert Sequence:

ATGCCAACCA ACACCATCCT TTTTGGAGCT GACGCTGCCA CCAGTTTCCT CCGCGCAGCT 
CGCTCAGGTA ATCTGGAAAA GGCTCTAGAC CACATCAAGA ACGGCATCGA CATCAACACA 
GCCAATCAGA ATGGACTCAA CGGGCTGCAT TTGGCCTCCA AGGAAGGCCA TGTTAAAATG 
GTGCTGGAGC TGCTTCACCA TGGTATTGTA CTTGAGACGA CAACGAAGAA AGGGAACACT 
GCTCTTCATA TAGCGGCGTT GGCTGGCCAG GAGCAGGTGG TCACTGAACT GGTCAACTAT 
GGAGCAAACG TCAACGCTCA GTCTCAGAAG GGCTTCACGC CGCTCTACAT GGCAGCACAA 
GAGAATCATC TGGAGGTTGT GAAGTTTCTG CTGGATAACG GAGCCAATCA GACCATTCCA 
ACTGAGGATG GCTTTACCCC TCTTGCTGTG GCTCTTCAGC AGGGCCATGA GAATGTGGTG 
GCCCTGCTCA TCAACCACGG TACTAAGGGC AAGGTCCGCC TTCCCGCACT GCACATCGCT 
GCCCGAAACG ACGACACCCG CACTGCCGCT GTGCTCTTAC AGAACGACCC CAATCCTGAC 
GTCCTGAGCA AGACAGGCTT CACGCCGCTC CACATCGCAG CACATTATGA AAACCTGAAT 
GTTGCTCAGC TTCTTCTGAA CCGAGGAGCT GATGTGAACT TCACACCTAA GAACGGGATC 
ACACCGCTGC ACATCGCATC CAGGAGAGGG AACGTCATCA TGGTCAGACT TCTGCTGGAC 
CGAGGAGCAA AGATCGATGC CAAGACAAAA GATGAGTTGA CTCCTCTTCA CTGTGCTGCC 
AGAAATGGCC ATGTGAGAAT CATCGAGATC CTTCTGGACC AAGGAGCCCC AATACAGGCC 
AAGACCAAAA ATGGCCTGTC GCCGATCCAC ATGGCAGCCC AGGGCGATCA CTTGGACTGT 
ATCCGACAGC TCTTACAGTA CAACGCAGAG ATCGATGACA TCACTCTGGA CCACCTGACA 
CCTCTGCATG TGGCGGCTCA CTGCGGGCAT CACCGCGTGG CCAAGGTGCT CCTGGATAAA 
GGAGCCAAAC CAAACACCCG CGCTCTGAAT GGATTCACGC CGCTCCACAT TGCCTGTAAG 
AAGAACCACA TGCGTGTAAT GGACCTGCTA TTAAAGCATT CTGCATCCCT GGAAGCTGTA 
ACCGAGTCAG GCTTGACCCC GCTGCATGTG TCCTCCTTCA TGGGTCATCT GAATATAGTC 
AAGATACTGA TGCAAAAAGG AGCTTCGCCG AACGCCTCAA ATGTGAAAGT GGAAACCCCT 
CTGCATATGG CATCTCGAGC TGGACACTGT GAAGTTGCTG AATTCCTATT GCAAAATGCA 
GCTCCAGTTG ATGCCAAAGC CAAGGATGAC CAGACGCCGC TACACTGTGC CTCCCGCATG 
GGCCACAACG AGATGGTGAA GTTGCTTCTG GAGCACAAGG CCAACTCAAA CTCCACCACC 
ACAGCAGGAC ACACACCGCT GCACATCGCG GCCCGCGAGG GACACACACA AACTGCCAGC 
ATCCTCCTGG ACATGAACGC CCAGCTCACC AAAATGACCA AGAAAGGCTT CACTCCTCTT 
CATGTTGCAG CCAAATACGG AAAGGTGGAC GTGGCAGTTC TGCTTCTGGA GAGGGGAGCA 
AATCCAAACG CTGCTGGAAA AGTTGGACTC ACGCCGCTGC ATGTGGCCGT CCATCATAAC 
AATCTAGATG TGGTCAATCT TTTACTCAGC AAAGGAGGAT CACCACACAG CGCCGCTCGG 
AATGGCTATA CTCCGCTCCA CATTGCTTCC AAACAGAACC AGGCGGAGGT GGCGAGCAGT 
TTACTGCAGC ACGGGGCGTC TGCCAATGCT GAGTCTCTGC AAGGGGTCAC ACCTCTACAC 
CTCGCCTCTC AAGAGGGTCA ACCTGACATG GTGTTACTGC TCATCTCCAA GCAGGCCAAT 
GTAAACCTTG GCAATAAGAG CGGTCTGACT CCTCTTCATC TTGTGGCTCA GGAAGGGCAT 
GTTGGGATTG CTGATATACT AGTGAAACAT GGAGCATCTG TTTATGCTGC CACACGGATG 
GGTTACACTC CGCTTCACGT GGCTTGTCAT TATGGGAATA TCAAGATGGT GAAGTTTCTC 
TTACAGCAGC AGGCCAACGT AAACTGCAAG ACCAGGATGA GCTACACGCC GCTACATCAG 
GCTGCTCAGC AGGGTCATAC GGACATCGTG ACGCTTCTGC TGAAACACGG AGCACAACCG 
AATGAAGTTA CATCTAACGG CACCTCTGCT CTTGCCATCG CCAAGCGGCT TGGATACATT 
TCAGTCATCG ATGTGCTCAA ACTGGTCACT GAAGAGACGG TCACTATGAC CACCACTGAA 
AAACATCGCA TGAGTTTCCC TGAAACCGTT GATGAGATCC TGGATGTGTC TGAAGATGAA 
GGAATTGCAC AGCTAACATT AGGCGAGGAG CTGCTGGGAA ACGAGGGCGC CAGGAATCAT 
TCTCCTGCTA TCCCGAGGAT CCCGTGTGTG TCTCCTGAGA CTGTCATGCT GAAAGAGCAT 
GAGATGGAGC AGCAGCACAC TCCACTTCCA TTGCCCAAAG AGTACGACGA GGATTCTCTG 
ATTCCCAGCA GTCCTGCAAC TGAGACTTCA GACAATGTCA GTCCGGTTGC CAGTCCCATC 
CACACCGGTT TTCTGGTGAG CTTCATGGTG GATGCTCGCG GCGGCTCGAT GCGCGGCAGC 
AGACACAACG GCCTCCGCGT CATCATTCCT CCTCGAACCT GCGCCGCTCC CACACGCATC 
ACCTGCCGCC TGGTGAAGCC CCAGAAGCTG CCCACACCGC CGCCATTAGT GGAGGGAGAG 
GGTCTCGCCA GCCGCATCAT CTCCCTTGGA CCCGCCAGCA TGCAGTTTTT AGGGCCTGTG 
ATTGTGGAGA TTCCTCATTT TGCGGCTCTC GGTCGGGGAG ATCGGGAACT CGTGGTCTTG 
AGGAGTGAGA ACGGATCGGT CTGGAAGGAA CATCGTAACC GATATGGAGA TGAAGTGCTA 
GAGACCATCC TCAACGGCAT GGATGAAGAT CTGGAGAGTC AGGAGGAGCT CGGGAAGAAA 
CGCATCAGAC GGATCATCTC CACCGACTTC CCGCTCTACT TTGCGGTGGT GTCTCGCATC 
CAGCAGGAGA ACGATCTGAT TGGCCCAGAA GGAGGTTTTC TGTCCAGTAA ACTGGTGCCG 
ATGGTCCAGG CCACCTTTCC AGAGACAGCC GTCACCAAAC GCGTGCGTCT GGGACTGCAG 
GCCCAGCCGG TGCCTGATGA GCTGGTGGCC AAGCTTTTGG GGAACCAGGC CACGTTCAGT 
CCTGTGGTGA CGGTGGAGCC TCGGCGCCGG AAGTTTCACC GTCCCATCGG CCTAAGAATC 
CCACTGCCAC CCTCATGGAG AGAGAGCCCG CGGGACGCCG GAGAGGGAGA CACCACCAGC 
CTGCGCCTGC TCTGCAGCGT CATCGGTGGA ACAGCTCCTG CCCAGTGGGA AGATATAACT 
GGCACCACCA AACTGGCGTA TTCCAATGAC TGTGCCAGTT TCACCACCAA CGTCTCTGCC 
CGATTCTGGC TGGCGGACTG TCCTCGGACT GCGGAGGCCG TGTCCTTTGC CAACCTCCTG 
TACCGCGAGC TGTCGGCCGT GCCGTACATG GCCAAGTTTG TAGTGTTTGC GAAGATGAAT 
GAAGCTCGCG AGGGTCGACT CCGCTGCTAC TGCATGACGG ATGACAAGAT GGACAAAACC 
CTGGAGCAGC ACGAGAACTT CTCGGAAGTG GCTCGCAGCA GAGACATCGA GGTGATGGAG 
GGAATGCCGC TGTATTTGGA GTGTTCAGGA AACTTGGTTC CTATAAGAAA AGCAGCCCAG 
CAGCCTCGCT GTTTCAGCTT CCAGGCCTTT AAAGACAACA GACTGCCTGT GTCCGTCAAG 
GTGAGGGACA GCAGTAAAGA CCCCTCTGGG TTTTTGTCTT TCTTGCGCAA GTCAACCAAA 
TATGAAGACA GCCAACACGT GCTGTGCAAC CTCAACGTGA CCATGCCGCC GTGCATTAAG 
ATTATGGGCA GCGATGAGCG GAGGAGGACT CTGACTCCTC TGGCTCTCAG AGAAAGATAC 
AGCGCTCTGA ATGAACCGGC AATGGCATCA CTGAGCGCCA TGGAAAGGAC GGAGTTAAAG 
ATGGCTTTAA TAGCTGAACA GCTAGGCCTG AGCTGGGCTG AGTTGGGACG GGAACTGCAG 
TTTAATGTAG ATGAAATAAA TAAGATCCGT GTGGAGAATC CCAACTCCCT TTTAGAGCAG 
AGCTCAACGC TGCTCAACCT GTGGGCCGCC CGAGAGGGGA AGAGAGCCAA AATGGACAGC 
CTGTACGTGG CCCTCAAGAG CATCGACCGA GTGGACATTG TGAACATGAT GGAGGGCCAG 
GGAGGACCAC CGGCGGGTCA GCAGGGGGGC TGGGAGCAGG ACGCTTCTAG GTCTAGGCAT 
CACGACCGAG ACCATCTCTC ACCTGGAATC ACAAATGGTT ACGGGCTGGT GGTGCAGGAG 
GAACTAGTGT CACCGGCCTC CATGCAGTAC AGTCTGCCCT CGCCACTGGG CAATGAGCCC 
TACTGGCAGG AGGTTTCCAG CATGGAGTGC GCGCCCATCG CCACCACTGA AGAAGACACG 
CTCATGGAGA TGTCCGAGGT ACAGGTTTGG CCCTCAGGAA ATAGTCCGTC CATTGTGGCT 
GTAGAGGACT CCTCATTGGA GTGCAGCAAT GCGGACGACT CCGAGGCGCT GCAGAACCGG 
AGCCTGGGTC ACTCCAGGAG CGGGACGCCC GGTGGGAACG GACTCACTCC GGGAGGTTTA 
AGTGGCTCCA TCGAGCTCCT GGAGGATCAG TCCATCGGTG CGGATTCAGA CTACAGTGCC 
GCCGGGATGC TGCGTCTGAG CGAAGGGGTC AACATCAGTG CTCTGGACAG AGGTCAGAGG 
TCGGAGAGTT CAGAAGGGGC TGTAGGTCAC CAAGTCACCA ACAACATTAA AGACGGTTCC 
AATGGAGGTT TGGGAGAAGG AGGAGGTGAC GGCACACAGT TAGAAGATAG CATATCTCTC 
ATTTCAGGGC AACAGCGTGT GTATGCTCGA ATGAGCGAGT CGCCTGGGCT GAGAAGACGG 
GCAGATCCTG GCGAAGACAG GTTAAGCGCA GGGTCGTTCC TGTCTTATCT GCAGGATCAG 
GGCTCTCCTG GTTGGCATTC GGCTGCAGAA GCGACTCAGA ACAGATTGGC GTCTTTGCAA 
TGCTCCCTTC CTAGGCAGGC AATAGAGTCT ATGATGTCAT CGGTGCGAGC TACGGTGGAC 
TCTGACTCCA AGCAGTCTTG TTTGGGCCAG GAGGCTCTGA TTGAGCCAGT GCGGGACATG 
GGGCACTCTG AGATCATCCA TGGCCATTAT CAGGGGACTC AGCCGTTTGA GAAAGGACTC 
GGCTTCCCGC ACAGAACAAC CAACATGCGC ACTTGGGAGG ACATGCGTCT GAGCGGACAG 
GGTGATGAGG CTGAGGATCT TCCAGGAGAG CAAGTGAGCG AGGAGCAATT CACAGACGAG 
CATGGCAACA TCGTCACCAA AAAGATTGTG AGGAAAGTGG TCCGGAGAGG AAAAGGGGAG 
GAAGGGGGTC AGGAGCTGAT CATTGAAGGT CTGCAGCAGG ATATCAGTGA GCCTGACTTT 
GATGGAGAAC AGTTCATGAG CTATGCCGTC CTGGGACGTG ACAGCAAGCC GGATGTTGTG 
GATGTGAAGA GAGGAGGTGC TCAGATAGTC AAATGTGCGA GTCTGCGGAG AGTAAAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_009302014.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009300289.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X7, mRNA.

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CloneID	ODa41286
Clone ID Related Accession (Same CDS sequence)	XM_009302014.2
Accession Version	XM_009302014.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5778bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ankyrin 1, erythrocytic a isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302014.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAACCA ACACCATCCT TTTTGGAGCT GACGCTGCCA CCAGTTTCCT CCGCGCAGCT 
CGCTCAGGTA ATCTGGAAAA GGCTCTAGAC CACATCAAGA ACGGCATCGA CATCAACACA 
GCCAATCAGA ATGGACTCAA CGGGCTGCAT TTGGCCTCCA AGGAAGGCCA TGTTAAAATG 
GTGCTGGAGC TGCTTCACCA TGGTATTGTA CTTGAGACGA CAACGAAGAA AGGGAACACT 
GCTCTTCATA TAGCGGCGTT GGCTGGCCAG GAGCAGGTGG TCACTGAACT GGTCAACTAT 
GGAGCAAACG TCAACGCTCA GTCTCAGAAG GGCTTCACGC CGCTCTACAT GGCAGCACAA 
GAGAATCATC TGGAGGTTGT GAAGTTTCTG CTGGATAACG GAGCCAATCA GACCATTCCA 
ACTGAGGATG GCTTTACCCC TCTTGCTGTG GCTCTTCAGC AGGGCCATGA GAATGTGGTG 
GCCCTGCTCA TCAACCACGG TACTAAGGGC AAGGTCCGCC TTCCCGCACT GCACATCGCT 
GCCCGAAACG ACGACACCCG CACTGCCGCT GTGCTCTTAC AGAACGACCC CAATCCTGAC 
GTCCTGAGCA AGACAGGCTT CACGCCGCTC CACATCGCAG CACATTATGA AAACCTGAAT 
GTTGCTCAGC TTCTTCTGAA CCGAGGAGCT GATGTGAACT TCACACCTAA GAACGGGATC 
ACACCGCTGC ACATCGCATC CAGGAGAGGG AACGTCATCA TGGTCAGACT TCTGCTGGAC 
CGAGGAGCAA AGATCGATGC CAAGACAAAA GATGAGTTGA CTCCTCTTCA CTGTGCTGCC 
AGAAATGGCC ATGTGAGAAT CATCGAGATC CTTCTGGACC AAGGAGCCCC AATACAGGCC 
AAGACCAAAA ATGGCCTGTC GCCGATCCAC ATGGCAGCCC AGGGCGATCA CTTGGACTGT 
ATCCGACAGC TCTTACAGTA CAACGCAGAG ATCGATGACA TCACTCTGGA CCACCTGACA 
CCTCTGCATG TGGCGGCTCA CTGCGGGCAT CACCGCGTGG CCAAGGTGCT CCTGGATAAA 
GGAGCCAAAC CAAACACCCG CGCTCTGAAT GGATTCACGC CGCTCCACAT TGCCTGTAAG 
AAGAACCACA TGCGTGTAAT GGACCTGCTA TTAAAGCATT CTGCATCCCT GGAAGCTGTA 
ACCGAGTCAG GCTTGACCCC GCTGCATGTG TCCTCCTTCA TGGGTCATCT GAATATAGTC 
AAGATACTGA TGCAAAAAGG AGCTTCGCCG AACGCCTCAA ATGTGAAAGT GGAAACCCCT 
CTGCATATGG CATCTCGAGC TGGACACTGT GAAGTTGCTG AATTCCTATT GCAAAATGCA 
GCTCCAGTTG ATGCCAAAGC CAAGGATGAC CAGACGCCGC TACACTGTGC CTCCCGCATG 
GGCCACAACG AGATGGTGAA GTTGCTTCTG GAGCACAAGG CCAACTCAAA CTCCACCACC 
ACAGCAGGAC ACACACCGCT GCACATCGCG GCCCGCGAGG GACACACACA AACTGCCAGC 
ATCCTCCTGG ACATGAACGC CCAGCTCACC AAAATGACCA AGAAAGGCTT CACTCCTCTT 
CATGTTGCAG CCAAATACGG AAAGGTGGAC GTGGCAGTTC TGCTTCTGGA GAGGGGAGCA 
AATCCAAACG CTGCTGGAAA AGTTGGACTC ACGCCGCTGC ATGTGGCCGT CCATCATAAC 
AATCTAGATG TGGTCAATCT TTTACTCAGC AAAGGAGGAT CACCACACAG CGCCGCTCGG 
AATGGCTATA CTCCGCTCCA CATTGCTTCC AAACAGAACC AGGCGGAGGT GGCGAGCAGT 
TTACTGCAGC ACGGGGCGTC TGCCAATGCT GAGTCTCTGC AAGGGGTCAC ACCTCTACAC 
CTCGCCTCTC AAGAGGGTCA ACCTGACATG GTGTTACTGC TCATCTCCAA GCAGGCCAAT 
GTAAACCTTG GCAATAAGAG CGGTCTGACT CCTCTTCATC TTGTGGCTCA GGAAGGGCAT 
GTTGGGATTG CTGATATACT AGTGAAACAT GGAGCATCTG TTTATGCTGC CACACGGATG 
GGTTACACTC CGCTTCACGT GGCTTGTCAT TATGGGAATA TCAAGATGGT GAAGTTTCTC 
TTACAGCAGC AGGCCAACGT AAACTGCAAG ACCAGGATGA GCTACACGCC GCTACATCAG 
GCTGCTCAGC AGGGTCATAC GGACATCGTG ACGCTTCTGC TGAAACACGG AGCACAACCG 
AATGAAGTTA CATCTAACGG CACCTCTGCT CTTGCCATCG CCAAGCGGCT TGGATACATT 
TCAGTCATCG ATGTGCTCAA ACTGGTCACT GAAGAGACGG TCACTATGAC CACCACTGAA 
AAACATCGCA TGAGTTTCCC TGAAACCGTT GATGAGATCC TGGATGTGTC TGAAGATGAA 
GGAATTGCAC AGCTAACATT AGGCGAGGAG CTGCTGGGAA ACGAGGGCGC CAGGTATTTG 
ATGAAGATGG AGGACATAAA GGACCATGAT GAGGATTTCC TTTCCCCCAA GAAAGTTCTA 
GAGTACGAGG GAGAGTTGGA GAATCATTCT CCTGCTATCC CGAGGATCCC GTGTGTGTCT 
CCTGAGACTG TCATGCTGAA AGAGCATGAG ATGGAGCAGC AGCACACTCC ACTTCCATTG 
CCCAAAGAGT ACGACGAGGA TTCTCTGATT CCCAGCAGTC CTGCAACTGA GACTTCAGAC 
AATGTCAGTC CGGTTGCCAG TCCCATCCAC ACCGGTTTTC TGGTGAGCTT CATGGTGGAT 
GCTCGCGGCG GCTCGATGCG CGGCAGCAGA CACAACGGCC TCCGCGTCAT CATTCCTCCT 
CGAACCTGCG CCGCTCCCAC ACGCATCACC TGCCGCCTGG TGAAGCCCCA GAAGCTGCCC 
ACACCGCCGC CATTAGTGGA GGGAGAGGGT CTCGCCAGCC GCATCATCTC CCTTGGACCC 
GCCAGCATGC AGTTTTTAGG GCCTGTGATT GTGGAGATTC CTCATTTTGC GGCTCTCGGT 
CGGGGAGATC GGGAACTCGT GGTCTTGAGG AGTGAGAACG GATCGGTCTG GAAGGAACAT 
CGTAACCGAT ATGGAGATGA AGTGCTAGAG ACCATCCTCA ACGGCATGGA TGAAGATCTG 
GAGAGTCAGG AGGAGCTCGG GAAGAAACGC ATCAGACGGA TCATCTCCAC CGACTTCCCG 
CTCTACTTTG CGGTGGTGTC TCGCATCCAG CAGGAGAACG ATCTGATTGG CCCAGAAGGA 
GGTTTTCTGT CCAGTAAACT GGTGCCGATG GTCCAGGCCA CCTTTCCAGA GACAGCCGTC 
ACCAAACGCG TGCGTCTGGG ACTGCAGGCC CAGCCGGTGC CTGATGAGCT GGTGGCCAAG 
CTTTTGGGGA ACCAGGCCAC GTTCAGTCCT GTGGTGACGG TGGAGCCTCG GCGCCGGAAG 
TTTCACCGTC CCATCGGCCT AAGAATCCCA CTGCCACCCT CATGGAGAGA GAGCCCGCGG 
GACGCCGGAG AGGGAGACAC CACCAGCCTG CGCCTGCTCT GCAGCGTCAT CGGTGGAACA 
GCTCCTGCCC AGTGGGAAGA TATAACTGGC ACCACCAAAC TGGCGTATTC CAATGACTGT 
GCCAGTTTCA CCACCAACGT CTCTGCCCGA TTCTGGCTGG CGGACTGTCC TCGGACTGCG 
GAGGCCGTGT CCTTTGCCAA CCTCCTGTAC CGCGAGCTGT CGGCCGTGCC GTACATGGCC 
AAGTTTGTAG TGTTTGCGAA GATGAATGAA GCTCGCGAGG GTCGACTCCG CTGCTACTGC 
ATGACGGATG ACAAGATGGA CAAAACCCTG GAGCAGCACG AGAACTTCTC GGAAGTGGCT 
CGCAGCAGAG ACATCGAGGT GATGGAGGGA ATGCCGCTGT ATTTGGAGTG TTCAGGAAAC 
TTGGTTCCTA TAAGAAAAGC AGCCCAGCAG CCTCGCTGTT TCAGCTTCCA GGCCTTTAAA 
GACAACAGAC TGCCTGTGTC CGTCAAGGTG AGGGACAGCA GTAAAGACCC CTCTGGGTTT 
TTGTCTTTCT TGCGCAAGTC AACCAAATAT GAAGACAGCC AACACGTGCT GTGCAACCTC 
AACGTGACCA TGCCGCCGTG CATTAAGATT ATGGGCAGCG ATGAGCGGAG GAGGACTCTG 
ACTCCTCTGG CTCTCAGAGA AAGATACAGC GCTCTGAATG AACCGGCAAT GGCATCACTG 
AGCGCCATGG AAAGGACGGA GTTAAAGATG GCTTTAATAG CTGAACAGCT AGGCCTGAGC 
TGGGCTGTGG ACAGCCTGTA CGTGGCCCTC AAGAGCATCG ACCGAGTGGA CATTGTGAAC 
ATGATGGAGG GCCAGGGAGG ACCACCGGCG GGTCAGCAGG GGGGCTGGGA GCAGGACGCT 
TCTAGGTCTA GGCATCACGA CCGAGACCAT CTCTCACCTG GAATCACAAA TGGTTACGGG 
CTGGTGGTGC AGGAGGAACT AGTGTCACCG GCCTCCATGC AGTACAGTCT GCCCTCGCCA 
CTGGGCAATG AGCCCTACTG GCAGGAGGTT TCCAGCATGG AGTGCGCGCC CATCGCCACC 
ACTGAAGAAG ACACGCTCAT GGAGATGTCC GAGGTACAGG TTTGGCCCTC AGGAAATAGT 
CCGTCCATTG TGGCTGTAGA GGACTCCTCA TTGGAGTGCA GCAATGCGGA CGACTCCGAG 
GCGCTGCAGA ACCGGAGCCT GGGTCACTCC AGGAGCGGGA CGCCCGGTGG GAACGGACTC 
ACTCCGGGAG GTTTAAGTGG CTCCATCGAG CTCCTGGAGG ATCAGTCCAT CGGTGCGGAT 
TCAGACTACA GTGCCGCCGG GATGCTGCGT CTGAGCGAAG GGGTCAACAT CAGTGCTCTG 
GACAGAGGTC AGAGGTCGGA GAGTTCAGAA GGGGCTGTAG GTCACCAAGT CACCAACAAC 
ATTAAAGACG GTTCCAATGG AGGTTTGGGA GAAGGAGGAG GTGACGGCAC ACAGTTAGAA 
GATAGCATAT CTCTCATTTC AGGGCAACAG CGTGTGTATG CTCGAATGAG CGAGTCGCCT 
GGGCTGAGAA GACGGGCAGA TCCTGGCGAA GACAGGTTAA GCGCAGGGTC GTTCCTGTCT 
TATCTGCAGG ATCAGGGCTC TCCTGGTTGG CATTCGGCTG CAGAAGCGAC TCAGAACAGA 
TTGGCGTCTT TGCAATGCTC CCTTCCTAGG CAGGCAATAG AGTCTATGAT GTCATCGGTG 
CGAGCTACGG TGGACTCTGA CTCCAAGCAG TCTTGTTTGG GCCAGGAGGC TCTGATTGAG 
CCAGTGCGGG ACATGGGGCA CTCTGAGATC ATCCATGGCC ATTATCAGGG GACTCAGCCG 
TTTGAGAAAG GACTCGGCTT CCCGCACAGA ACAACCAACA TGCGCACTTG GGAGGACATG 
CGTCTGAGCG GACAGGGTGA TGAGGCTGAG GATCTTCCAG GAGAGCAAGT GAGCGAGGAG 
CAATTCACAG ACGAGCATGG CAACATCGTC ACCAAAAAGA TTGTGAGGAA AGTGGTCCGG 
AGAGGAAAAG GGGAGGAAGG GGGTCAGGAG CTGATCATTG AAGGTCTGCA GCAGGATATC 
AGTGAGCCTG ACTTTGATGG AGAACAGTTC ATGAGCTATG CCGTCCTGGG ACGTGACAGC 
AAGCCGGATG TTGTGGATGT GAAGAGAGGA GGTGCTCAGA TAGTCAAATG TGCGAGTCTG 
CGGAGAGTAA AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300289.1
CDS	342..6119
Translation	MPTNTILFGADAATSFLRAARSGNLEKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLINHGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGLTPLHVSSFMGHLNIVKILMQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGANPNAAGKVGLTPLHVAVHHNNLDVVNLLLSKGGSPHSAARNGYTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPLHLASQEGQPDMVLLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKHGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSNGTSALAIAKRLGYISVIDVLKLVTEETVTMTTTEKHRMSFPETVDEILDVSEDEGIAQLTLGEELLGNEGARYLMKMEDIKDHDEDFLSPKKVLEYEGELENHSPAIPRIPCVSPETVMLKEHEMEQQHTPLPLPKEYDEDSLIPSSPATETSDNVSPVASPIHTGFLVSFMVDARGGSMRGSRHNGLRVIIPPRTCAAPTRITCRLVKPQKLPTPPPLVEGEGLASRIISLGPASMQFLGPVIVEIPHFAALGRGDRELVVLRSENGSVWKEHRNRYGDEVLETILNGMDEDLESQEELGKKRIRRIISTDFPLYFAVVSRIQQENDLIGPEGGFLSSKLVPMVQATFPETAVTKRVRLGLQAQPVPDELVAKLLGNQATFSPVVTVEPRRRKFHRPIGLRIPLPPSWRESPRDAGEGDTTSLRLLCSVIGGTAPAQWEDITGTTKLAYSNDCASFTTNVSARFWLADCPRTAEAVSFANLLYRELSAVPYMAKFVVFAKMNEAREGRLRCYCMTDDKMDKTLEQHENFSEVARSRDIEVMEGMPLYLECSGNLVPIRKAAQQPRCFSFQAFKDNRLPVSVKVRDSSKDPSGFLSFLRKSTKYEDSQHVLCNLNVTMPPCIKIMGSDERRRTLTPLALRERYSALNEPAMASLSAMERTELKMALIAEQLGLSWAVDSLYVALKSIDRVDIVNMMEGQGGPPAGQQGGWEQDASRSRHHDRDHLSPGITNGYGLVVQEELVSPASMQYSLPSPLGNEPYWQEVSSMECAPIATTEEDTLMEMSEVQVWPSGNSPSIVAVEDSSLECSNADDSEALQNRSLGHSRSGTPGGNGLTPGGLSGSIELLEDQSIGADSDYSAAGMLRLSEGVNISALDRGQRSESSEGAVGHQVTNNIKDGSNGGLGEGGGDGTQLEDSISLISGQQRVYARMSESPGLRRRADPGEDRLSAGSFLSYLQDQGSPGWHSAAEATQNRLASLQCSLPRQAIESMMSSVRATVDSDSKQSCLGQEALIEPVRDMGHSEIIHGHYQGTQPFEKGLGFPHRTTNMRTWEDMRLSGQGDEAEDLPGEQVSEEQFTDEHGNIVTKKIVRKVVRRGKGEEGGQELIIEGLQQDISEPDFDGEQFMSYAVLGRDSKPDVVDVKRGGAQIVKCASLRRVKQ

Target ORF information:

RefSeq Version	XM_009302014.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302014.2

ORF Insert Sequence:

ATGCCAACCA ACACCATCCT TTTTGGAGCT GACGCTGCCA CCAGTTTCCT CCGCGCAGCT 
CGCTCAGGTA ATCTGGAAAA GGCTCTAGAC CACATCAAGA ACGGCATCGA CATCAACACA 
GCCAATCAGA ATGGACTCAA CGGGCTGCAT TTGGCCTCCA AGGAAGGCCA TGTTAAAATG 
GTGCTGGAGC TGCTTCACCA TGGTATTGTA CTTGAGACGA CAACGAAGAA AGGGAACACT 
GCTCTTCATA TAGCGGCGTT GGCTGGCCAG GAGCAGGTGG TCACTGAACT GGTCAACTAT 
GGAGCAAACG TCAACGCTCA GTCTCAGAAG GGCTTCACGC CGCTCTACAT GGCAGCACAA 
GAGAATCATC TGGAGGTTGT GAAGTTTCTG CTGGATAACG GAGCCAATCA GACCATTCCA 
ACTGAGGATG GCTTTACCCC TCTTGCTGTG GCTCTTCAGC AGGGCCATGA GAATGTGGTG 
GCCCTGCTCA TCAACCACGG TACTAAGGGC AAGGTCCGCC TTCCCGCACT GCACATCGCT 
GCCCGAAACG ACGACACCCG CACTGCCGCT GTGCTCTTAC AGAACGACCC CAATCCTGAC 
GTCCTGAGCA AGACAGGCTT CACGCCGCTC CACATCGCAG CACATTATGA AAACCTGAAT 
GTTGCTCAGC TTCTTCTGAA CCGAGGAGCT GATGTGAACT TCACACCTAA GAACGGGATC 
ACACCGCTGC ACATCGCATC CAGGAGAGGG AACGTCATCA TGGTCAGACT TCTGCTGGAC 
CGAGGAGCAA AGATCGATGC CAAGACAAAA GATGAGTTGA CTCCTCTTCA CTGTGCTGCC 
AGAAATGGCC ATGTGAGAAT CATCGAGATC CTTCTGGACC AAGGAGCCCC AATACAGGCC 
AAGACCAAAA ATGGCCTGTC GCCGATCCAC ATGGCAGCCC AGGGCGATCA CTTGGACTGT 
ATCCGACAGC TCTTACAGTA CAACGCAGAG ATCGATGACA TCACTCTGGA CCACCTGACA 
CCTCTGCATG TGGCGGCTCA CTGCGGGCAT CACCGCGTGG CCAAGGTGCT CCTGGATAAA 
GGAGCCAAAC CAAACACCCG CGCTCTGAAT GGATTCACGC CGCTCCACAT TGCCTGTAAG 
AAGAACCACA TGCGTGTAAT GGACCTGCTA TTAAAGCATT CTGCATCCCT GGAAGCTGTA 
ACCGAGTCAG GCTTGACCCC GCTGCATGTG TCCTCCTTCA TGGGTCATCT GAATATAGTC 
AAGATACTGA TGCAAAAAGG AGCTTCGCCG AACGCCTCAA ATGTGAAAGT GGAAACCCCT 
CTGCATATGG CATCTCGAGC TGGACACTGT GAAGTTGCTG AATTCCTATT GCAAAATGCA 
GCTCCAGTTG ATGCCAAAGC CAAGGATGAC CAGACGCCGC TACACTGTGC CTCCCGCATG 
GGCCACAACG AGATGGTGAA GTTGCTTCTG GAGCACAAGG CCAACTCAAA CTCCACCACC 
ACAGCAGGAC ACACACCGCT GCACATCGCG GCCCGCGAGG GACACACACA AACTGCCAGC 
ATCCTCCTGG ACATGAACGC CCAGCTCACC AAAATGACCA AGAAAGGCTT CACTCCTCTT 
CATGTTGCAG CCAAATACGG AAAGGTGGAC GTGGCAGTTC TGCTTCTGGA GAGGGGAGCA 
AATCCAAACG CTGCTGGAAA AGTTGGACTC ACGCCGCTGC ATGTGGCCGT CCATCATAAC 
AATCTAGATG TGGTCAATCT TTTACTCAGC AAAGGAGGAT CACCACACAG CGCCGCTCGG 
AATGGCTATA CTCCGCTCCA CATTGCTTCC AAACAGAACC AGGCGGAGGT GGCGAGCAGT 
TTACTGCAGC ACGGGGCGTC TGCCAATGCT GAGTCTCTGC AAGGGGTCAC ACCTCTACAC 
CTCGCCTCTC AAGAGGGTCA ACCTGACATG GTGTTACTGC TCATCTCCAA GCAGGCCAAT 
GTAAACCTTG GCAATAAGAG CGGTCTGACT CCTCTTCATC TTGTGGCTCA GGAAGGGCAT 
GTTGGGATTG CTGATATACT AGTGAAACAT GGAGCATCTG TTTATGCTGC CACACGGATG 
GGTTACACTC CGCTTCACGT GGCTTGTCAT TATGGGAATA TCAAGATGGT GAAGTTTCTC 
TTACAGCAGC AGGCCAACGT AAACTGCAAG ACCAGGATGA GCTACACGCC GCTACATCAG 
GCTGCTCAGC AGGGTCATAC GGACATCGTG ACGCTTCTGC TGAAACACGG AGCACAACCG 
AATGAAGTTA CATCTAACGG CACCTCTGCT CTTGCCATCG CCAAGCGGCT TGGATACATT 
TCAGTCATCG ATGTGCTCAA ACTGGTCACT GAAGAGACGG TCACTATGAC CACCACTGAA 
AAACATCGCA TGAGTTTCCC TGAAACCGTT GATGAGATCC TGGATGTGTC TGAAGATGAA 
GGAATTGCAC AGCTAACATT AGGCGAGGAG CTGCTGGGAA ACGAGGGCGC CAGGTATTTG 
ATGAAGATGG AGGACATAAA GGACCATGAT GAGGATTTCC TTTCCCCCAA GAAAGTTCTA 
GAGTACGAGG GAGAGTTGGA GAATCATTCT CCTGCTATCC CGAGGATCCC GTGTGTGTCT 
CCTGAGACTG TCATGCTGAA AGAGCATGAG ATGGAGCAGC AGCACACTCC ACTTCCATTG 
CCCAAAGAGT ACGACGAGGA TTCTCTGATT CCCAGCAGTC CTGCAACTGA GACTTCAGAC 
AATGTCAGTC CGGTTGCCAG TCCCATCCAC ACCGGTTTTC TGGTGAGCTT CATGGTGGAT 
GCTCGCGGCG GCTCGATGCG CGGCAGCAGA CACAACGGCC TCCGCGTCAT CATTCCTCCT 
CGAACCTGCG CCGCTCCCAC ACGCATCACC TGCCGCCTGG TGAAGCCCCA GAAGCTGCCC 
ACACCGCCGC CATTAGTGGA GGGAGAGGGT CTCGCCAGCC GCATCATCTC CCTTGGACCC 
GCCAGCATGC AGTTTTTAGG GCCTGTGATT GTGGAGATTC CTCATTTTGC GGCTCTCGGT 
CGGGGAGATC GGGAACTCGT GGTCTTGAGG AGTGAGAACG GATCGGTCTG GAAGGAACAT 
CGTAACCGAT ATGGAGATGA AGTGCTAGAG ACCATCCTCA ACGGCATGGA TGAAGATCTG 
GAGAGTCAGG AGGAGCTCGG GAAGAAACGC ATCAGACGGA TCATCTCCAC CGACTTCCCG 
CTCTACTTTG CGGTGGTGTC TCGCATCCAG CAGGAGAACG ATCTGATTGG CCCAGAAGGA 
GGTTTTCTGT CCAGTAAACT GGTGCCGATG GTCCAGGCCA CCTTTCCAGA GACAGCCGTC 
ACCAAACGCG TGCGTCTGGG ACTGCAGGCC CAGCCGGTGC CTGATGAGCT GGTGGCCAAG 
CTTTTGGGGA ACCAGGCCAC GTTCAGTCCT GTGGTGACGG TGGAGCCTCG GCGCCGGAAG 
TTTCACCGTC CCATCGGCCT AAGAATCCCA CTGCCACCCT CATGGAGAGA GAGCCCGCGG 
GACGCCGGAG AGGGAGACAC CACCAGCCTG CGCCTGCTCT GCAGCGTCAT CGGTGGAACA 
GCTCCTGCCC AGTGGGAAGA TATAACTGGC ACCACCAAAC TGGCGTATTC CAATGACTGT 
GCCAGTTTCA CCACCAACGT CTCTGCCCGA TTCTGGCTGG CGGACTGTCC TCGGACTGCG 
GAGGCCGTGT CCTTTGCCAA CCTCCTGTAC CGCGAGCTGT CGGCCGTGCC GTACATGGCC 
AAGTTTGTAG TGTTTGCGAA GATGAATGAA GCTCGCGAGG GTCGACTCCG CTGCTACTGC 
ATGACGGATG ACAAGATGGA CAAAACCCTG GAGCAGCACG AGAACTTCTC GGAAGTGGCT 
CGCAGCAGAG ACATCGAGGT GATGGAGGGA ATGCCGCTGT ATTTGGAGTG TTCAGGAAAC 
TTGGTTCCTA TAAGAAAAGC AGCCCAGCAG CCTCGCTGTT TCAGCTTCCA GGCCTTTAAA 
GACAACAGAC TGCCTGTGTC CGTCAAGGTG AGGGACAGCA GTAAAGACCC CTCTGGGTTT 
TTGTCTTTCT TGCGCAAGTC AACCAAATAT GAAGACAGCC AACACGTGCT GTGCAACCTC 
AACGTGACCA TGCCGCCGTG CATTAAGATT ATGGGCAGCG ATGAGCGGAG GAGGACTCTG 
ACTCCTCTGG CTCTCAGAGA AAGATACAGC GCTCTGAATG AACCGGCAAT GGCATCACTG 
AGCGCCATGG AAAGGACGGA GTTAAAGATG GCTTTAATAG CTGAACAGCT AGGCCTGAGC 
TGGGCTGTGG ACAGCCTGTA CGTGGCCCTC AAGAGCATCG ACCGAGTGGA CATTGTGAAC 
ATGATGGAGG GCCAGGGAGG ACCACCGGCG GGTCAGCAGG GGGGCTGGGA GCAGGACGCT 
TCTAGGTCTA GGCATCACGA CCGAGACCAT CTCTCACCTG GAATCACAAA TGGTTACGGG 
CTGGTGGTGC AGGAGGAACT AGTGTCACCG GCCTCCATGC AGTACAGTCT GCCCTCGCCA 
CTGGGCAATG AGCCCTACTG GCAGGAGGTT TCCAGCATGG AGTGCGCGCC CATCGCCACC 
ACTGAAGAAG ACACGCTCAT GGAGATGTCC GAGGTACAGG TTTGGCCCTC AGGAAATAGT 
CCGTCCATTG TGGCTGTAGA GGACTCCTCA TTGGAGTGCA GCAATGCGGA CGACTCCGAG 
GCGCTGCAGA ACCGGAGCCT GGGTCACTCC AGGAGCGGGA CGCCCGGTGG GAACGGACTC 
ACTCCGGGAG GTTTAAGTGG CTCCATCGAG CTCCTGGAGG ATCAGTCCAT CGGTGCGGAT 
TCAGACTACA GTGCCGCCGG GATGCTGCGT CTGAGCGAAG GGGTCAACAT CAGTGCTCTG 
GACAGAGGTC AGAGGTCGGA GAGTTCAGAA GGGGCTGTAG GTCACCAAGT CACCAACAAC 
ATTAAAGACG GTTCCAATGG AGGTTTGGGA GAAGGAGGAG GTGACGGCAC ACAGTTAGAA 
GATAGCATAT CTCTCATTTC AGGGCAACAG CGTGTGTATG CTCGAATGAG CGAGTCGCCT 
GGGCTGAGAA GACGGGCAGA TCCTGGCGAA GACAGGTTAA GCGCAGGGTC GTTCCTGTCT 
TATCTGCAGG ATCAGGGCTC TCCTGGTTGG CATTCGGCTG CAGAAGCGAC TCAGAACAGA 
TTGGCGTCTT TGCAATGCTC CCTTCCTAGG CAGGCAATAG AGTCTATGAT GTCATCGGTG 
CGAGCTACGG TGGACTCTGA CTCCAAGCAG TCTTGTTTGG GCCAGGAGGC TCTGATTGAG 
CCAGTGCGGG ACATGGGGCA CTCTGAGATC ATCCATGGCC ATTATCAGGG GACTCAGCCG 
TTTGAGAAAG GACTCGGCTT CCCGCACAGA ACAACCAACA TGCGCACTTG GGAGGACATG 
CGTCTGAGCG GACAGGGTGA TGAGGCTGAG GATCTTCCAG GAGAGCAAGT GAGCGAGGAG 
CAATTCACAG ACGAGCATGG CAACATCGTC ACCAAAAAGA TTGTGAGGAA AGTGGTCCGG 
AGAGGAAAAG GGGAGGAAGG GGGTCAGGAG CTGATCATTG AAGGTCTGCA GCAGGATATC 
AGTGAGCCTG ACTTTGATGG AGAACAGTTC ATGAGCTATG CCGTCCTGGG ACGTGACAGC 
AAGCCGGATG TTGTGGATGT GAAGAGAGGA GGTGCTCAGA TAGTCAAATG TGCGAGTCTG 
CGGAGAGTAA AGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_009302015.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009300290.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X10, mRNA.

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CloneID	ODa41287
Clone ID Related Accession (Same CDS sequence)	XM_009302015.2
Accession Version	XM_009302015.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 5682bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ankyrin 1, erythrocytic a isoform X10
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302015.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAACCA ACACCATCCT TTTTGGAGCT GACGCTGCCA CCAGTTTCCT CCGCGCAGCT 
CGCTCAGGTA ATCTGGAAAA GGCTCTAGAC CACATCAAGA ACGGCATCGA CATCAACACA 
GCCAATCAGA ATGGACTCAA CGGGCTGCAT TTGGCCTCCA AGGAAGGCCA TGTTAAAATG 
GTGCTGGAGC TGCTTCACCA TGGTATTGTA CTTGAGACGA CAACGAAGAA AGGGAACACT 
GCTCTTCATA TAGCGGCGTT GGCTGGCCAG GAGCAGGTGG TCACTGAACT GGTCAACTAT 
GGAGCAAACG TCAACGCTCA GTCTCAGAAG GGCTTCACGC CGCTCTACAT GGCAGCACAA 
GAGAATCATC TGGAGGTTGT GAAGTTTCTG CTGGATAACG GAGCCAATCA GACCATTCCA 
ACTGAGGATG GCTTTACCCC TCTTGCTGTG GCTCTTCAGC AGGGCCATGA GAATGTGGTG 
GCCCTGCTCA TCAACCACGG TACTAAGGGC AAGGTCCGCC TTCCCGCACT GCACATCGCT 
GCCCGAAACG ACGACACCCG CACTGCCGCT GTGCTCTTAC AGAACGACCC CAATCCTGAC 
GTCCTGAGCA AGACAGGCTT CACGCCGCTC CACATCGCAG CACATTATGA AAACCTGAAT 
GTTGCTCAGC TTCTTCTGAA CCGAGGAGCT GATGTGAACT TCACACCTAA GAACGGGATC 
ACACCGCTGC ACATCGCATC CAGGAGAGGG AACGTCATCA TGGTCAGACT TCTGCTGGAC 
CGAGGAGCAA AGATCGATGC CAAGACAAAA GATGAGTTGA CTCCTCTTCA CTGTGCTGCC 
AGAAATGGCC ATGTGAGAAT CATCGAGATC CTTCTGGACC AAGGAGCCCC AATACAGGCC 
AAGACCAAAA ATGGCCTGTC GCCGATCCAC ATGGCAGCCC AGGGCGATCA CTTGGACTGT 
ATCCGACAGC TCTTACAGTA CAACGCAGAG ATCGATGACA TCACTCTGGA CCACCTGACA 
CCTCTGCATG TGGCGGCTCA CTGCGGGCAT CACCGCGTGG CCAAGGTGCT CCTGGATAAA 
GGAGCCAAAC CAAACACCCG CGCTCTGAAT GGATTCACGC CGCTCCACAT TGCCTGTAAG 
AAGAACCACA TGCGTGTAAT GGACCTGCTA TTAAAGCATT CTGCATCCCT GGAAGCTGTA 
ACCGAGTCAG GCTTGACCCC GCTGCATGTG TCCTCCTTCA TGGGTCATCT GAATATAGTC 
AAGATACTGA TGCAAAAAGG AGCTTCGCCG AACGCCTCAA ATGTGAAAGT GGAAACCCCT 
CTGCATATGG CATCTCGAGC TGGACACTGT GAAGTTGCTG AATTCCTATT GCAAAATGCA 
GCTCCAGTTG ATGCCAAAGC CAAGGATGAC CAGACGCCGC TACACTGTGC CTCCCGCATG 
GGCCACAACG AGATGGTGAA GTTGCTTCTG GAGCACAAGG CCAACTCAAA CTCCACCACC 
ACAGCAGGAC ACACACCGCT GCACATCGCG GCCCGCGAGG GACACACACA AACTGCCAGC 
ATCCTCCTGG ACATGAACGC CCAGCTCACC AAAATGACCA AGAAAGGCTT CACTCCTCTT 
CATGTTGCAG CCAAATACGG AAAGGTGGAC GTGGCAGTTC TGCTTCTGGA GAGGGGAGCA 
AATCCAAACG CTGCTGGAAA AGTTGGACTC ACGCCGCTGC ATGTGGCCGT CCATCATAAC 
AATCTAGATG TGGTCAATCT TTTACTCAGC AAAGGAGGAT CACCACACAG CGCCGCTCGG 
AATGGCTATA CTCCGCTCCA CATTGCTTCC AAACAGAACC AGGCGGAGGT GGCGAGCAGT 
TTACTGCAGC ACGGGGCGTC TGCCAATGCT GAGTCTCTGC AAGGGGTCAC ACCTCTACAC 
CTCGCCTCTC AAGAGGGTCA ACCTGACATG GTGTTACTGC TCATCTCCAA GCAGGCCAAT 
GTAAACCTTG GCAATAAGAG CGGTCTGACT CCTCTTCATC TTGTGGCTCA GGAAGGGCAT 
GTTGGGATTG CTGATATACT AGTGAAACAT GGAGCATCTG TTTATGCTGC CACACGGATG 
GGTTACACTC CGCTTCACGT GGCTTGTCAT TATGGGAATA TCAAGATGGT GAAGTTTCTC 
TTACAGCAGC AGGCCAACGT AAACTGCAAG ACCAGGATGA GCTACACGCC GCTACATCAG 
GCTGCTCAGC AGGGTCATAC GGACATCGTG ACGCTTCTGC TGAAACACGG AGCACAACCG 
AATGAAGTTA CATCTAACGG CACCTCTGCT CTTGCCATCG CCAAGCGGCT TGGATACATT 
TCAGTCATCG ATGTGCTCAA ACTGGTCACT GAAGAGACGG TCACTATGAC CACCACTGAA 
AAACATCGCA TGAGTTTCCC TGAAACCGTT GATGAGATCC TGGATGTGTC TGAAGATGAA 
GGAATTGCAC AGCTAACATT AGGCGAGGAG CTGCTGGGAA ACGAGGGCGC CAGGTATTTG 
ATGAAGATGG AGGACATAAA GGACCATGAT GAGGATTTCC TTTCCCCCAA GAAAGTTCTA 
GAGTACGAGG GAGAGTTGGA GAATCATTCT CCTGCTATCC CGAGGATCCC GTGTGTGTCT 
CCTGAGACTG TCATGCTGAA AGAGCATGAG ATGGAGCAGC AGCACACTCC ACTTCCATTG 
CCCAAAGAGT ACGACGAGGA TTCTCTGATT CCCAGCAGTC CTGCAACTGA GACTTCAGAC 
AATGTCAGTC CGGTTGCCAG TCCCATCCAC ACCGGTTTTC TGGTGAGCTT CATGGTGGAT 
GCTCGCGGCG GCTCGATGCG CGGCAGCAGA CACAACGGCC TCCGCGTCAT CATTCCTCCT 
CGAACCTGCG CCGCTCCCAC ACGCATCACC TGCCGCCTGG TGAAGCCCCA GAAGCTGCCC 
ACACCGCCGC CATTAGTGGA GGGAGAGGGT CTCGCCAGCC GCATCATCTC CCTTGGACCC 
GCCAGCATGC AGTTTTTAGG GCCTGTGATT GTGGAGATTC CTCATTTTGC GGCTCTCGGT 
CGGGGAGATC GGGAACTCGT GGTCTTGAGG AGTGAGAACG GATCGGTCTG GAAGGAACAT 
CGTAACCGAT ATGGAGATGA AGTGCTAGAG ACCATCCTCA ACGGCATGGA TGAAGATCTG 
GAGAGTCAGG AGGAGCTCGG GAAGAAACGC ATCAGACGGA TCATCTCCAC CGACTTCCCG 
CTCTACTTTG CGGTGGTGTC TCGCATCCAG CAGGAGAACG ATCTGATTGG CCCAGAAGGA 
GGTTTTCTGT CCAGTAAACT GGTGCCGATG GTCCAGGCCA CCTTTCCAGA GACAGCCGTC 
ACCAAACGCG TGCGTCTGGG ACTGCAGGCC CAGCCGGTGC CTGATGAGCT GGTGGCCAAG 
CTTTTGGGGA ACCAGGCCAC GTTCAGTCCT GTGGTGACGG TGGAGCCTCG GCGCCGGAAG 
TTTCACCGTC CCATCGGCCT AAGAATCCCA CTGCCACCCT CATGGAGAGA GAGCCCGCGG 
GACGCCGGAG AGGGAGACAC CACCAGCCTG CGCCTGCTCT GCAGCGTCAT CGGTGGAACA 
GCTCCTGCCC AGTGGGAAGA TATAACTGGC ACCACCAAAC TGGCGTATTC CAATGACTGT 
GCCAGTTTCA CCACCAACGT CTCTGCCCGA TTCTGGCTGG CGGACTGTCC TCGGACTGCG 
GAGGCCGTGT CCTTTGCCAA CCTCCTGTAC CGCGAGCTGT CGGCCGTGCC GTACATGGCC 
AAGTTTGTAG TGTTTGCGAA GATGAATGAA GCTCGCGAGG GTCGACTCCG CTGCTACTGC 
ATGACGGATG ACAAGATGGA CAAAACCCTG GAGCAGCACG AGAACTTCTC GGAAGTGGCT 
CGCAGCAGAG ACATCGAGGT GATGGAGGGA ATGCCGCTGT ATTTGGAGTG TTCAGGAAAC 
TTGGTTCCTA TAAGAAAAGC AGCCCAGCAG CCTCGCTGTT TCAGCTTCCA GGCCTTTAAA 
GACAACAGAC TGCCTGTGTC CGTCAAGGTG AGGGACAGCA GTAAAGACCC CTCTGGGTTT 
TTGTCTTTCT TGCGCAAGTC AACCAAATAT GAAGACAGCC AACACGTGCT GTGCAACCTC 
AACGTGACCA TGCCGCCGTG CATTAAGATT ATGGGCAGCG ATGAGCGGAG GAGGACTCTG 
ACTCCTCTGG CTCTCAGAGA AAGATACAGC GCTCTGAATG AACCGGCAAT GGCATCACTG 
AGCGCCATGG AAAGGACGGA GTTAAAGATG GCTTTAATAG CTGAACAGCT AGGCCTGAGC 
TGGGCTGAGT TGGGACGGGA ACTGCAGTTT AATGTAGATG AAATAAATAA GATCCGTGTG 
GAGAATCCCA ACTCCCTTTT AGAGCAGAGC TCAACGCTGC TCAACCTGTG GGCCGCCCGA 
GAGGGGAAGA GAGCCAAAAT GGACAGCCTG TACGTGGCCC TCAAGAGCAT CGACCGAGTG 
GACATTGTGA ACATGATGGA GGGCCAGGGA GGACCACCGG CGGGTCAGCA GGGGGGCTGG 
GAGCAGGACG CTTCTAGGTC TAGGCATCAC GACCGAGACC ATCTCTCACC TGGAATCACA 
AATGGTTACG GGCTGGTGGT GCAGGAGGAA CTAGTGTCAC CGGCCTCCAT GCAGTACAGT 
CTGCCCTCGC CACTGGGCAA TGAGCCCTAC TGGCAGGAGG TTTCCAGCAT GGAGTGCGCG 
CCCATCGCCA CCACTGAAGA AGACACGCTC ATGGAGATGT CCGAGGTACA GGTTTGGCCC 
TCAGGAAATA GTCCGTCCAT TGTGGCTGTA GAGGACTCCT CATTGGAGTG CAGCAATGCG 
GACGACTCCG AGGCGCTGCA GAACCGGAGC CTGGGTCACT CCAGGAGCGG GACGCCCGGT 
GGGAACGGAC TCACTCCGGG AGGTTTAAGT GGCTCCATCG AGCTCCTGGA GGATCAGTCC 
ATCGGTGCGG ATTCAGACTA CAGTGCCGCC GGGATGCTGC GTCTGAGCGA AGGGGTCAAC 
ATCAGTGCTC TGGACAGAGG TCAGAGGTCG GAGAGTTCAG AAGGGGCTGT AGGTCACCAA 
GTCACCAACA ACATTAAAGA CGGTTCCAAT GGAGGTTTGG GAGAAGGAGG AGGTGACGGC 
ACACAGTTAG AAGATAGCAT ATCTCTCATT TCAGGGCAAC AGCGTGTGTA TGCTCGAATG 
AGCGAGTCGC CTGGGCTGAG AAGACGGGCA GATCCTGGCG AAGACAGGTT AAGCGCAGGG 
TCGTTCCTGT CTTATCTGCA GGATCAGGGC TCTCCTGGTT GGCATTCGGC TGCAGAAGCG 
ACTCAGAACA GATTGGCGTC TTTGCAATGC TCCCTTCCTA GGCAGGCAAT AGAGTCTATG 
ATGTCATCGG TGCGAGCTAC GGTGGACTCT GACTCCAAGC AGTCTTGTTT GGGCCAGGAG 
GCTCTGATTG AGCCAGTGCG GGACATGGGG CACTCTGAGA TCATCCATGG CCATTATCAG 
GGGACTCAGC CGTTTGAGAA AGGACTCGGC TTCCCGCACA GAACAACCAA CATGCGCACT 
TGGGAGGACA TGCGTCTGAG CGGACAGCCG GATGTTGTGG ATGTGAAGAG AGGAGGTGCT 
CAGATAGTCA AATGTGCGAG TCTGCGGAGA GTAAAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300290.1
CDS	345..6026
Translation	MPTNTILFGADAATSFLRAARSGNLEKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLINHGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGLTPLHVSSFMGHLNIVKILMQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGANPNAAGKVGLTPLHVAVHHNNLDVVNLLLSKGGSPHSAARNGYTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPLHLASQEGQPDMVLLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKHGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSNGTSALAIAKRLGYISVIDVLKLVTEETVTMTTTEKHRMSFPETVDEILDVSEDEGIAQLTLGEELLGNEGARYLMKMEDIKDHDEDFLSPKKVLEYEGELENHSPAIPRIPCVSPETVMLKEHEMEQQHTPLPLPKEYDEDSLIPSSPATETSDNVSPVASPIHTGFLVSFMVDARGGSMRGSRHNGLRVIIPPRTCAAPTRITCRLVKPQKLPTPPPLVEGEGLASRIISLGPASMQFLGPVIVEIPHFAALGRGDRELVVLRSENGSVWKEHRNRYGDEVLETILNGMDEDLESQEELGKKRIRRIISTDFPLYFAVVSRIQQENDLIGPEGGFLSSKLVPMVQATFPETAVTKRVRLGLQAQPVPDELVAKLLGNQATFSPVVTVEPRRRKFHRPIGLRIPLPPSWRESPRDAGEGDTTSLRLLCSVIGGTAPAQWEDITGTTKLAYSNDCASFTTNVSARFWLADCPRTAEAVSFANLLYRELSAVPYMAKFVVFAKMNEAREGRLRCYCMTDDKMDKTLEQHENFSEVARSRDIEVMEGMPLYLECSGNLVPIRKAAQQPRCFSFQAFKDNRLPVSVKVRDSSKDPSGFLSFLRKSTKYEDSQHVLCNLNVTMPPCIKIMGSDERRRTLTPLALRERYSALNEPAMASLSAMERTELKMALIAEQLGLSWAELGRELQFNVDEINKIRVENPNSLLEQSSTLLNLWAAREGKRAKMDSLYVALKSIDRVDIVNMMEGQGGPPAGQQGGWEQDASRSRHHDRDHLSPGITNGYGLVVQEELVSPASMQYSLPSPLGNEPYWQEVSSMECAPIATTEEDTLMEMSEVQVWPSGNSPSIVAVEDSSLECSNADDSEALQNRSLGHSRSGTPGGNGLTPGGLSGSIELLEDQSIGADSDYSAAGMLRLSEGVNISALDRGQRSESSEGAVGHQVTNNIKDGSNGGLGEGGGDGTQLEDSISLISGQQRVYARMSESPGLRRRADPGEDRLSAGSFLSYLQDQGSPGWHSAAEATQNRLASLQCSLPRQAIESMMSSVRATVDSDSKQSCLGQEALIEPVRDMGHSEIIHGHYQGTQPFEKGLGFPHRTTNMRTWEDMRLSGQPDVVDVKRGGAQIVKCASLRRVKQ

Target ORF information:

RefSeq Version	XM_009302015.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302015.2

ORF Insert Sequence:

ATGCCAACCA ACACCATCCT TTTTGGAGCT GACGCTGCCA CCAGTTTCCT CCGCGCAGCT 
CGCTCAGGTA ATCTGGAAAA GGCTCTAGAC CACATCAAGA ACGGCATCGA CATCAACACA 
GCCAATCAGA ATGGACTCAA CGGGCTGCAT TTGGCCTCCA AGGAAGGCCA TGTTAAAATG 
GTGCTGGAGC TGCTTCACCA TGGTATTGTA CTTGAGACGA CAACGAAGAA AGGGAACACT 
GCTCTTCATA TAGCGGCGTT GGCTGGCCAG GAGCAGGTGG TCACTGAACT GGTCAACTAT 
GGAGCAAACG TCAACGCTCA GTCTCAGAAG GGCTTCACGC CGCTCTACAT GGCAGCACAA 
GAGAATCATC TGGAGGTTGT GAAGTTTCTG CTGGATAACG GAGCCAATCA GACCATTCCA 
ACTGAGGATG GCTTTACCCC TCTTGCTGTG GCTCTTCAGC AGGGCCATGA GAATGTGGTG 
GCCCTGCTCA TCAACCACGG TACTAAGGGC AAGGTCCGCC TTCCCGCACT GCACATCGCT 
GCCCGAAACG ACGACACCCG CACTGCCGCT GTGCTCTTAC AGAACGACCC CAATCCTGAC 
GTCCTGAGCA AGACAGGCTT CACGCCGCTC CACATCGCAG CACATTATGA AAACCTGAAT 
GTTGCTCAGC TTCTTCTGAA CCGAGGAGCT GATGTGAACT TCACACCTAA GAACGGGATC 
ACACCGCTGC ACATCGCATC CAGGAGAGGG AACGTCATCA TGGTCAGACT TCTGCTGGAC 
CGAGGAGCAA AGATCGATGC CAAGACAAAA GATGAGTTGA CTCCTCTTCA CTGTGCTGCC 
AGAAATGGCC ATGTGAGAAT CATCGAGATC CTTCTGGACC AAGGAGCCCC AATACAGGCC 
AAGACCAAAA ATGGCCTGTC GCCGATCCAC ATGGCAGCCC AGGGCGATCA CTTGGACTGT 
ATCCGACAGC TCTTACAGTA CAACGCAGAG ATCGATGACA TCACTCTGGA CCACCTGACA 
CCTCTGCATG TGGCGGCTCA CTGCGGGCAT CACCGCGTGG CCAAGGTGCT CCTGGATAAA 
GGAGCCAAAC CAAACACCCG CGCTCTGAAT GGATTCACGC CGCTCCACAT TGCCTGTAAG 
AAGAACCACA TGCGTGTAAT GGACCTGCTA TTAAAGCATT CTGCATCCCT GGAAGCTGTA 
ACCGAGTCAG GCTTGACCCC GCTGCATGTG TCCTCCTTCA TGGGTCATCT GAATATAGTC 
AAGATACTGA TGCAAAAAGG AGCTTCGCCG AACGCCTCAA ATGTGAAAGT GGAAACCCCT 
CTGCATATGG CATCTCGAGC TGGACACTGT GAAGTTGCTG AATTCCTATT GCAAAATGCA 
GCTCCAGTTG ATGCCAAAGC CAAGGATGAC CAGACGCCGC TACACTGTGC CTCCCGCATG 
GGCCACAACG AGATGGTGAA GTTGCTTCTG GAGCACAAGG CCAACTCAAA CTCCACCACC 
ACAGCAGGAC ACACACCGCT GCACATCGCG GCCCGCGAGG GACACACACA AACTGCCAGC 
ATCCTCCTGG ACATGAACGC CCAGCTCACC AAAATGACCA AGAAAGGCTT CACTCCTCTT 
CATGTTGCAG CCAAATACGG AAAGGTGGAC GTGGCAGTTC TGCTTCTGGA GAGGGGAGCA 
AATCCAAACG CTGCTGGAAA AGTTGGACTC ACGCCGCTGC ATGTGGCCGT CCATCATAAC 
AATCTAGATG TGGTCAATCT TTTACTCAGC AAAGGAGGAT CACCACACAG CGCCGCTCGG 
AATGGCTATA CTCCGCTCCA CATTGCTTCC AAACAGAACC AGGCGGAGGT GGCGAGCAGT 
TTACTGCAGC ACGGGGCGTC TGCCAATGCT GAGTCTCTGC AAGGGGTCAC ACCTCTACAC 
CTCGCCTCTC AAGAGGGTCA ACCTGACATG GTGTTACTGC TCATCTCCAA GCAGGCCAAT 
GTAAACCTTG GCAATAAGAG CGGTCTGACT CCTCTTCATC TTGTGGCTCA GGAAGGGCAT 
GTTGGGATTG CTGATATACT AGTGAAACAT GGAGCATCTG TTTATGCTGC CACACGGATG 
GGTTACACTC CGCTTCACGT GGCTTGTCAT TATGGGAATA TCAAGATGGT GAAGTTTCTC 
TTACAGCAGC AGGCCAACGT AAACTGCAAG ACCAGGATGA GCTACACGCC GCTACATCAG 
GCTGCTCAGC AGGGTCATAC GGACATCGTG ACGCTTCTGC TGAAACACGG AGCACAACCG 
AATGAAGTTA CATCTAACGG CACCTCTGCT CTTGCCATCG CCAAGCGGCT TGGATACATT 
TCAGTCATCG ATGTGCTCAA ACTGGTCACT GAAGAGACGG TCACTATGAC CACCACTGAA 
AAACATCGCA TGAGTTTCCC TGAAACCGTT GATGAGATCC TGGATGTGTC TGAAGATGAA 
GGAATTGCAC AGCTAACATT AGGCGAGGAG CTGCTGGGAA ACGAGGGCGC CAGGTATTTG 
ATGAAGATGG AGGACATAAA GGACCATGAT GAGGATTTCC TTTCCCCCAA GAAAGTTCTA 
GAGTACGAGG GAGAGTTGGA GAATCATTCT CCTGCTATCC CGAGGATCCC GTGTGTGTCT 
CCTGAGACTG TCATGCTGAA AGAGCATGAG ATGGAGCAGC AGCACACTCC ACTTCCATTG 
CCCAAAGAGT ACGACGAGGA TTCTCTGATT CCCAGCAGTC CTGCAACTGA GACTTCAGAC 
AATGTCAGTC CGGTTGCCAG TCCCATCCAC ACCGGTTTTC TGGTGAGCTT CATGGTGGAT 
GCTCGCGGCG GCTCGATGCG CGGCAGCAGA CACAACGGCC TCCGCGTCAT CATTCCTCCT 
CGAACCTGCG CCGCTCCCAC ACGCATCACC TGCCGCCTGG TGAAGCCCCA GAAGCTGCCC 
ACACCGCCGC CATTAGTGGA GGGAGAGGGT CTCGCCAGCC GCATCATCTC CCTTGGACCC 
GCCAGCATGC AGTTTTTAGG GCCTGTGATT GTGGAGATTC CTCATTTTGC GGCTCTCGGT 
CGGGGAGATC GGGAACTCGT GGTCTTGAGG AGTGAGAACG GATCGGTCTG GAAGGAACAT 
CGTAACCGAT ATGGAGATGA AGTGCTAGAG ACCATCCTCA ACGGCATGGA TGAAGATCTG 
GAGAGTCAGG AGGAGCTCGG GAAGAAACGC ATCAGACGGA TCATCTCCAC CGACTTCCCG 
CTCTACTTTG CGGTGGTGTC TCGCATCCAG CAGGAGAACG ATCTGATTGG CCCAGAAGGA 
GGTTTTCTGT CCAGTAAACT GGTGCCGATG GTCCAGGCCA CCTTTCCAGA GACAGCCGTC 
ACCAAACGCG TGCGTCTGGG ACTGCAGGCC CAGCCGGTGC CTGATGAGCT GGTGGCCAAG 
CTTTTGGGGA ACCAGGCCAC GTTCAGTCCT GTGGTGACGG TGGAGCCTCG GCGCCGGAAG 
TTTCACCGTC CCATCGGCCT AAGAATCCCA CTGCCACCCT CATGGAGAGA GAGCCCGCGG 
GACGCCGGAG AGGGAGACAC CACCAGCCTG CGCCTGCTCT GCAGCGTCAT CGGTGGAACA 
GCTCCTGCCC AGTGGGAAGA TATAACTGGC ACCACCAAAC TGGCGTATTC CAATGACTGT 
GCCAGTTTCA CCACCAACGT CTCTGCCCGA TTCTGGCTGG CGGACTGTCC TCGGACTGCG 
GAGGCCGTGT CCTTTGCCAA CCTCCTGTAC CGCGAGCTGT CGGCCGTGCC GTACATGGCC 
AAGTTTGTAG TGTTTGCGAA GATGAATGAA GCTCGCGAGG GTCGACTCCG CTGCTACTGC 
ATGACGGATG ACAAGATGGA CAAAACCCTG GAGCAGCACG AGAACTTCTC GGAAGTGGCT 
CGCAGCAGAG ACATCGAGGT GATGGAGGGA ATGCCGCTGT ATTTGGAGTG TTCAGGAAAC 
TTGGTTCCTA TAAGAAAAGC AGCCCAGCAG CCTCGCTGTT TCAGCTTCCA GGCCTTTAAA 
GACAACAGAC TGCCTGTGTC CGTCAAGGTG AGGGACAGCA GTAAAGACCC CTCTGGGTTT 
TTGTCTTTCT TGCGCAAGTC AACCAAATAT GAAGACAGCC AACACGTGCT GTGCAACCTC 
AACGTGACCA TGCCGCCGTG CATTAAGATT ATGGGCAGCG ATGAGCGGAG GAGGACTCTG 
ACTCCTCTGG CTCTCAGAGA AAGATACAGC GCTCTGAATG AACCGGCAAT GGCATCACTG 
AGCGCCATGG AAAGGACGGA GTTAAAGATG GCTTTAATAG CTGAACAGCT AGGCCTGAGC 
TGGGCTGAGT TGGGACGGGA ACTGCAGTTT AATGTAGATG AAATAAATAA GATCCGTGTG 
GAGAATCCCA ACTCCCTTTT AGAGCAGAGC TCAACGCTGC TCAACCTGTG GGCCGCCCGA 
GAGGGGAAGA GAGCCAAAAT GGACAGCCTG TACGTGGCCC TCAAGAGCAT CGACCGAGTG 
GACATTGTGA ACATGATGGA GGGCCAGGGA GGACCACCGG CGGGTCAGCA GGGGGGCTGG 
GAGCAGGACG CTTCTAGGTC TAGGCATCAC GACCGAGACC ATCTCTCACC TGGAATCACA 
AATGGTTACG GGCTGGTGGT GCAGGAGGAA CTAGTGTCAC CGGCCTCCAT GCAGTACAGT 
CTGCCCTCGC CACTGGGCAA TGAGCCCTAC TGGCAGGAGG TTTCCAGCAT GGAGTGCGCG 
CCCATCGCCA CCACTGAAGA AGACACGCTC ATGGAGATGT CCGAGGTACA GGTTTGGCCC 
TCAGGAAATA GTCCGTCCAT TGTGGCTGTA GAGGACTCCT CATTGGAGTG CAGCAATGCG 
GACGACTCCG AGGCGCTGCA GAACCGGAGC CTGGGTCACT CCAGGAGCGG GACGCCCGGT 
GGGAACGGAC TCACTCCGGG AGGTTTAAGT GGCTCCATCG AGCTCCTGGA GGATCAGTCC 
ATCGGTGCGG ATTCAGACTA CAGTGCCGCC GGGATGCTGC GTCTGAGCGA AGGGGTCAAC 
ATCAGTGCTC TGGACAGAGG TCAGAGGTCG GAGAGTTCAG AAGGGGCTGT AGGTCACCAA 
GTCACCAACA ACATTAAAGA CGGTTCCAAT GGAGGTTTGG GAGAAGGAGG AGGTGACGGC 
ACACAGTTAG AAGATAGCAT ATCTCTCATT TCAGGGCAAC AGCGTGTGTA TGCTCGAATG 
AGCGAGTCGC CTGGGCTGAG AAGACGGGCA GATCCTGGCG AAGACAGGTT AAGCGCAGGG 
TCGTTCCTGT CTTATCTGCA GGATCAGGGC TCTCCTGGTT GGCATTCGGC TGCAGAAGCG 
ACTCAGAACA GATTGGCGTC TTTGCAATGC TCCCTTCCTA GGCAGGCAAT AGAGTCTATG 
ATGTCATCGG TGCGAGCTAC GGTGGACTCT GACTCCAAGC AGTCTTGTTT GGGCCAGGAG 
GCTCTGATTG AGCCAGTGCG GGACATGGGG CACTCTGAGA TCATCCATGG CCATTATCAG 
GGGACTCAGC CGTTTGAGAA AGGACTCGGC TTCCCGCACA GAACAACCAA CATGCGCACT 
TGGGAGGACA TGCGTCTGAG CGGACAGCCG GATGTTGTGG ATGTGAAGAG AGGAGGTGCT 
CAGATAGTCA AATGTGCGAG TCTGCGGAGA GTAAAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_009302016.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009300291.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X11, mRNA.

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CloneID	ODa41288
Clone ID Related Accession (Same CDS sequence)	XM_009302016.2
Accession Version	XM_009302016.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4668bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ankyrin 1, erythrocytic a isoform X11
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302016.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAACCA ACACCATCCT TTTTGGAGCT GACGCTGCCA CCAGTTTCCT CCGCGCAGCT 
CGCTCAGGTA ATCTGGAAAA GGCTCTAGAC CACATCAAGA ACGGCATCGA CATCAACACA 
GCCAATCAGA ATGGACTCAA CGGGCTGCAT TTGGCCTCCA AGGAAGGCCA TGTTAAAATG 
GTGCTGGAGC TGCTTCACCA TGGTATTGTA CTTGAGACGA CAACGAAGAA AGGGAACACT 
GCTCTTCATA TAGCGGCGTT GGCTGGCCAG GAGCAGGTGG TCACTGAACT GGTCAACTAT 
GGAGCAAACG TCAACGCTCA GTCTCAGAAG GGCTTCACGC CGCTCTACAT GGCAGCACAA 
GAGAATCATC TGGAGGTTGT GAAGTTTCTG CTGGATAACG GAGCCAATCA GACCATTCCA 
ACTGAGGATG GCTTTACCCC TCTTGCTGTG GCTCTTCAGC AGGGCCATGA GAATGTGGTG 
GCCCTGCTCA TCAACCACGG TACTAAGGGC AAGGTCCGCC TTCCCGCACT GCACATCGCT 
GCCCGAAACG ACGACACCCG CACTGCCGCT GTGCTCTTAC AGAACGACCC CAATCCTGAC 
GTCCTGAGCA AGACAGGCTT CACGCCGCTC CACATCGCAG CACATTATGA AAACCTGAAT 
GTTGCTCAGC TTCTTCTGAA CCGAGGAGCT GATGTGAACT TCACACCTAA GAACGGGATC 
ACACCGCTGC ACATCGCATC CAGGAGAGGG AACGTCATCA TGGTCAGACT TCTGCTGGAC 
CGAGGAGCAA AGATCGATGC CAAGACAAAA GATGAGTTGA CTCCTCTTCA CTGTGCTGCC 
AGAAATGGCC ATGTGAGAAT CATCGAGATC CTTCTGGACC AAGGAGCCCC AATACAGGCC 
AAGACCAAAA ATGGCCTGTC GCCGATCCAC ATGGCAGCCC AGGGCGATCA CTTGGACTGT 
ATCCGACAGC TCTTACAGTA CAACGCAGAG ATCGATGACA TCACTCTGGA CCACCTGACA 
CCTCTGCATG TGGCGGCTCA CTGCGGGCAT CACCGCGTGG CCAAGGTGCT CCTGGATAAA 
GGAGCCAAAC CAAACACCCG CGCTCTGAAT GGATTCACGC CGCTCCACAT TGCCTGTAAG 
AAGAACCACA TGCGTGTAAT GGACCTGCTA TTAAAGCATT CTGCATCCCT GGAAGCTGTA 
ACCGAGTCAG GCTTGACCCC GCTGCATGTG TCCTCCTTCA TGGGTCATCT GAATATAGTC 
AAGATACTGA TGCAAAAAGG AGCTTCGCCG AACGCCTCAA ATGTGAAAGT GGAAACCCCT 
CTGCATATGG CATCTCGAGC TGGACACTGT GAAGTTGCTG AATTCCTATT GCAAAATGCA 
GCTCCAGTTG ATGCCAAAGC CAAGGATGAC CAGACGCCGC TACACTGTGC CTCCCGCATG 
GGCCACAACG AGATGGTGAA GTTGCTTCTG GAGCACAAGG CCAACTCAAA CTCCACCACC 
ACAGCAGGAC ACACACCGCT GCACATCGCG GCCCGCGAGG GACACACACA AACTGCCAGC 
ATCCTCCTGG ACATGAACGC CCAGCTCACC AAAATGACCA AGAAAGGCTT CACTCCTCTT 
CATGTTGCAG CCAAATACGG AAAGGTGGAC GTGGCAGTTC TGCTTCTGGA GAGGGGAGCA 
AATCCAAACG CTGCTGGAAA AGTTGGACTC ACGCCGCTGC ATGTGGCCGT CCATCATAAC 
AATCTAGATG TGGTCAATCT TTTACTCAGC AAAGGAGGAT CACCACACAG CGCCGCTCGG 
AATGGCTATA CTCCGCTCCA CATTGCTTCC AAACAGAACC AGGCGGAGGT GGCGAGCAGT 
TTACTGCAGC ACGGGGCGTC TGCCAATGCT GAGTCTCTGC AAGGGGTCAC ACCTCTACAC 
CTCGCCTCTC AAGAGGGTCA ACCTGACATG GTGTTACTGC TCATCTCCAA GCAGGCCAAT 
GTAAACCTTG GCAATAAGAG CGGTCTGACT CCTCTTCATC TTGTGGCTCA GGAAGGGCAT 
GTTGGGATTG CTGATATACT AGTGAAACAT GGAGCATCTG TTTATGCTGC CACACGGATG 
GGTTACACTC CGCTTCACGT GGCTTGTCAT TATGGGAATA TCAAGATGGT GAAGTTTCTC 
TTACAGCAGC AGGCCAACGT AAACTGCAAG ACCAGGATGA GCTACACGCC GCTACATCAG 
GCTGCTCAGC AGGGTCATAC GGACATCGTG ACGCTTCTGC TGAAACACGG AGCACAACCG 
AATGAAGTTA CATCTAACGG CACCTCTGCT CTTGCCATCG CCAAGCGGCT TGGATACATT 
TCAGTCATCG ATGTGCTCAA ACTGGTCACT GAAGAGACGG TCACTATGAC CACCACTGAA 
AAACATCGCA TGAGTTTCCC TGAAACCGTT GATGAGATCC TGGATGTGTC TGAAGATGAA 
GGAATTGCAC AGCTAACATT AGGCGAGGAG CTGCTGGGAA ACGAGGGCGC CAGGTATTTG 
ATGAAGATGG AGGACATAAA GGACCATGAT GAGGATTTCC TTTCCCCCAA GAAAGTTCTA 
GAGTACGAGG GAGAGTTGGA GAATCATTCT CCTGCTATCC CGAGGATCCC GTGTGTGTCT 
CCTGAGACTG TCATGCTGAA AGAGCATGAG ATGGAGCAGC AGCACACTCC ACTTCCATTG 
CCCAAAGAGT ACGACGAGGA TTCTCTGATT CCCAGCAGTC CTGCAACTGA GACTTCAGAC 
AATGTCAGTC CGGTTGCCAG TCCCATCCAC ACCGGTTTTC TGGTGAGCTT CATGGTGGAT 
GCTCGCGGCG GCTCGATGCG CGGCAGCAGA CACAACGGCC TCCGCGTCAT CATTCCTCCT 
CGAACCTGCG CCGCTCCCAC ACGCATCACC TGCCGCCTGG TGAAGCCCCA GAAGCTGCCC 
ACACCGCCGC CATTAGTGGA GGGAGAGGGT CTCGCCAGCC GCATCATCTC CCTTGGACCC 
GCCAGCATGC AGTTTTTAGG GCCTGTGATT GTGGAGATTC CTCATTTTGC GGCTCTCGGT 
CGGGGAGATC GGGAACTCGT GGTCTTGAGG AGTGAGAACG GATCGGTCTG GAAGGAACAT 
CGTAACCGAT ATGGAGATGA AGTGCTAGAG ACCATCCTCA ACGGCATGGA TGAAGATCTG 
GAGAGTCAGG AGGAGCTCGG GAAGAAACGC ATCAGACGGA TCATCTCCAC CGACTTCCCG 
CTCTACTTTG CGGTGGTGTC TCGCATCCAG CAGGAGAACG ATCTGATTGG CCCAGAAGGA 
GGTTTTCTGT CCAGTAAACT GGTGCCGATG GTCCAGGCCA CCTTTCCAGA GACAGCCGTC 
ACCAAACGCG TGCGTCTGGG ACTGCAGGCC CAGCCGGTGC CTGATGAGCT GGTGGCCAAG 
CTTTTGGGGA ACCAGGCCAC GTTCAGTCCT GTGGTGACGG TGGAGCCTCG GCGCCGGAAG 
TTTCACCGTC CCATCGGCCT AAGAATCCCA CTGCCACCCT CATGGAGAGA GAGCCCGCGG 
GACGCCGGAG AGGGAGACAC CACCAGCCTG CGCCTGCTCT GCAGCGTCAT CGGTGGAACA 
GCTCCTGCCC AGTGGGAAGA TATAACTGGC ACCACCAAAC TGGCGTATTC CAATGACTGT 
GCCAGTTTCA CCACCAACGT CTCTGCCCGA TTCTGGCTGG CGGACTGTCC TCGGACTGCG 
GAGGCCGTGT CCTTTGCCAA CCTCCTGTAC CGCGAGCTGT CGGCCGTGCC GTACATGGCC 
AAGTTTGTAG TGTTTGCGAA GATGAATGAA GCTCGCGAGG GTCGACTCCG CTGCTACTGC 
ATGACGGATG ACAAGATGGA CAAAACCCTG GAGCAGCACG AGAACTTCTC GGAAGTGGCT 
CGCAGCAGAG ACATCGAGGT GATGGAGGGA ATGCCGCTGT ATTTGGAGTG TTCAGGAAAC 
TTGGTTCCTA TAAGAAAAGC AGCCCAGCAG CCTCGCTGTT TCAGCTTCCA GGCCTTTAAA 
GACAACAGAC TGCCTGTGTC CGTCAAGGTG AGGGACAGCA GTAAAGACCC CTCTGGGTTT 
TTGTCTTTCT TGCGCAAGTC AACCAAATAT GAAGACAGCC AACACGTGCT GTGCAACCTC 
AACGTGACCA TGCCGCCGTG CATTAAGATT ATGGGCAGCG ATGAGCGGAG GAGGACTCTG 
ACTCCTCTGG CTCTCAGAGA AAGATACAGC GCTCTGAATG AACCGGCAAT GGCATCACTG 
AGCGCCATGG AAAGGACGGA GTTAAAGATG GCTTTAATAG CTGAACAGCT AGGCCTGAGC 
TGGGCTGAGT TGGGACGGGA ACTGCAGTTT AATGTAGATG AAATAAATAA GATCCGTGTG 
GAGAATCCCA ACTCCCTTTT AGAGCAGAGC TCAACGCTGC TCAACCTGTG GGCCGCCCGA 
GAGGGGAAGA GAGCCAAAAT GGCGTGTCTC TGTCCTCATC CTTTATGTCC AACTACCCTG 
TCTTCCCCTT CTCCATTTCT CCACCATCTG TGTGTCTCTC ACCACTCTAG TGGACAGCCT 
GTACGTGGCC CTCAAGAGCA TCGACCGAGT GGACATTGTG AACATGATGG AGGGCCAGGG 
AGGACCACCG GCGGGTCAGC AGGGGGGCTG GGAGCAGGAC GCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300291.1
CDS	338..5005
Translation	MPTNTILFGADAATSFLRAARSGNLEKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLINHGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGLTPLHVSSFMGHLNIVKILMQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGANPNAAGKVGLTPLHVAVHHNNLDVVNLLLSKGGSPHSAARNGYTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPLHLASQEGQPDMVLLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKHGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSNGTSALAIAKRLGYISVIDVLKLVTEETVTMTTTEKHRMSFPETVDEILDVSEDEGIAQLTLGEELLGNEGARYLMKMEDIKDHDEDFLSPKKVLEYEGELENHSPAIPRIPCVSPETVMLKEHEMEQQHTPLPLPKEYDEDSLIPSSPATETSDNVSPVASPIHTGFLVSFMVDARGGSMRGSRHNGLRVIIPPRTCAAPTRITCRLVKPQKLPTPPPLVEGEGLASRIISLGPASMQFLGPVIVEIPHFAALGRGDRELVVLRSENGSVWKEHRNRYGDEVLETILNGMDEDLESQEELGKKRIRRIISTDFPLYFAVVSRIQQENDLIGPEGGFLSSKLVPMVQATFPETAVTKRVRLGLQAQPVPDELVAKLLGNQATFSPVVTVEPRRRKFHRPIGLRIPLPPSWRESPRDAGEGDTTSLRLLCSVIGGTAPAQWEDITGTTKLAYSNDCASFTTNVSARFWLADCPRTAEAVSFANLLYRELSAVPYMAKFVVFAKMNEAREGRLRCYCMTDDKMDKTLEQHENFSEVARSRDIEVMEGMPLYLECSGNLVPIRKAAQQPRCFSFQAFKDNRLPVSVKVRDSSKDPSGFLSFLRKSTKYEDSQHVLCNLNVTMPPCIKIMGSDERRRTLTPLALRERYSALNEPAMASLSAMERTELKMALIAEQLGLSWAELGRELQFNVDEINKIRVENPNSLLEQSSTLLNLWAAREGKRAKMACLCPHPLCPTTLSSPSPFLHHLCVSHHSSGQPVRGPQEHRPSGHCEHDGGPGRTTGGSAGGLGAGRF

Target ORF information:

RefSeq Version	XM_009302016.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302016.2

ORF Insert Sequence:

ATGCCAACCA ACACCATCCT TTTTGGAGCT GACGCTGCCA CCAGTTTCCT CCGCGCAGCT 
CGCTCAGGTA ATCTGGAAAA GGCTCTAGAC CACATCAAGA ACGGCATCGA CATCAACACA 
GCCAATCAGA ATGGACTCAA CGGGCTGCAT TTGGCCTCCA AGGAAGGCCA TGTTAAAATG 
GTGCTGGAGC TGCTTCACCA TGGTATTGTA CTTGAGACGA CAACGAAGAA AGGGAACACT 
GCTCTTCATA TAGCGGCGTT GGCTGGCCAG GAGCAGGTGG TCACTGAACT GGTCAACTAT 
GGAGCAAACG TCAACGCTCA GTCTCAGAAG GGCTTCACGC CGCTCTACAT GGCAGCACAA 
GAGAATCATC TGGAGGTTGT GAAGTTTCTG CTGGATAACG GAGCCAATCA GACCATTCCA 
ACTGAGGATG GCTTTACCCC TCTTGCTGTG GCTCTTCAGC AGGGCCATGA GAATGTGGTG 
GCCCTGCTCA TCAACCACGG TACTAAGGGC AAGGTCCGCC TTCCCGCACT GCACATCGCT 
GCCCGAAACG ACGACACCCG CACTGCCGCT GTGCTCTTAC AGAACGACCC CAATCCTGAC 
GTCCTGAGCA AGACAGGCTT CACGCCGCTC CACATCGCAG CACATTATGA AAACCTGAAT 
GTTGCTCAGC TTCTTCTGAA CCGAGGAGCT GATGTGAACT TCACACCTAA GAACGGGATC 
ACACCGCTGC ACATCGCATC CAGGAGAGGG AACGTCATCA TGGTCAGACT TCTGCTGGAC 
CGAGGAGCAA AGATCGATGC CAAGACAAAA GATGAGTTGA CTCCTCTTCA CTGTGCTGCC 
AGAAATGGCC ATGTGAGAAT CATCGAGATC CTTCTGGACC AAGGAGCCCC AATACAGGCC 
AAGACCAAAA ATGGCCTGTC GCCGATCCAC ATGGCAGCCC AGGGCGATCA CTTGGACTGT 
ATCCGACAGC TCTTACAGTA CAACGCAGAG ATCGATGACA TCACTCTGGA CCACCTGACA 
CCTCTGCATG TGGCGGCTCA CTGCGGGCAT CACCGCGTGG CCAAGGTGCT CCTGGATAAA 
GGAGCCAAAC CAAACACCCG CGCTCTGAAT GGATTCACGC CGCTCCACAT TGCCTGTAAG 
AAGAACCACA TGCGTGTAAT GGACCTGCTA TTAAAGCATT CTGCATCCCT GGAAGCTGTA 
ACCGAGTCAG GCTTGACCCC GCTGCATGTG TCCTCCTTCA TGGGTCATCT GAATATAGTC 
AAGATACTGA TGCAAAAAGG AGCTTCGCCG AACGCCTCAA ATGTGAAAGT GGAAACCCCT 
CTGCATATGG CATCTCGAGC TGGACACTGT GAAGTTGCTG AATTCCTATT GCAAAATGCA 
GCTCCAGTTG ATGCCAAAGC CAAGGATGAC CAGACGCCGC TACACTGTGC CTCCCGCATG 
GGCCACAACG AGATGGTGAA GTTGCTTCTG GAGCACAAGG CCAACTCAAA CTCCACCACC 
ACAGCAGGAC ACACACCGCT GCACATCGCG GCCCGCGAGG GACACACACA AACTGCCAGC 
ATCCTCCTGG ACATGAACGC CCAGCTCACC AAAATGACCA AGAAAGGCTT CACTCCTCTT 
CATGTTGCAG CCAAATACGG AAAGGTGGAC GTGGCAGTTC TGCTTCTGGA GAGGGGAGCA 
AATCCAAACG CTGCTGGAAA AGTTGGACTC ACGCCGCTGC ATGTGGCCGT CCATCATAAC 
AATCTAGATG TGGTCAATCT TTTACTCAGC AAAGGAGGAT CACCACACAG CGCCGCTCGG 
AATGGCTATA CTCCGCTCCA CATTGCTTCC AAACAGAACC AGGCGGAGGT GGCGAGCAGT 
TTACTGCAGC ACGGGGCGTC TGCCAATGCT GAGTCTCTGC AAGGGGTCAC ACCTCTACAC 
CTCGCCTCTC AAGAGGGTCA ACCTGACATG GTGTTACTGC TCATCTCCAA GCAGGCCAAT 
GTAAACCTTG GCAATAAGAG CGGTCTGACT CCTCTTCATC TTGTGGCTCA GGAAGGGCAT 
GTTGGGATTG CTGATATACT AGTGAAACAT GGAGCATCTG TTTATGCTGC CACACGGATG 
GGTTACACTC CGCTTCACGT GGCTTGTCAT TATGGGAATA TCAAGATGGT GAAGTTTCTC 
TTACAGCAGC AGGCCAACGT AAACTGCAAG ACCAGGATGA GCTACACGCC GCTACATCAG 
GCTGCTCAGC AGGGTCATAC GGACATCGTG ACGCTTCTGC TGAAACACGG AGCACAACCG 
AATGAAGTTA CATCTAACGG CACCTCTGCT CTTGCCATCG CCAAGCGGCT TGGATACATT 
TCAGTCATCG ATGTGCTCAA ACTGGTCACT GAAGAGACGG TCACTATGAC CACCACTGAA 
AAACATCGCA TGAGTTTCCC TGAAACCGTT GATGAGATCC TGGATGTGTC TGAAGATGAA 
GGAATTGCAC AGCTAACATT AGGCGAGGAG CTGCTGGGAA ACGAGGGCGC CAGGTATTTG 
ATGAAGATGG AGGACATAAA GGACCATGAT GAGGATTTCC TTTCCCCCAA GAAAGTTCTA 
GAGTACGAGG GAGAGTTGGA GAATCATTCT CCTGCTATCC CGAGGATCCC GTGTGTGTCT 
CCTGAGACTG TCATGCTGAA AGAGCATGAG ATGGAGCAGC AGCACACTCC ACTTCCATTG 
CCCAAAGAGT ACGACGAGGA TTCTCTGATT CCCAGCAGTC CTGCAACTGA GACTTCAGAC 
AATGTCAGTC CGGTTGCCAG TCCCATCCAC ACCGGTTTTC TGGTGAGCTT CATGGTGGAT 
GCTCGCGGCG GCTCGATGCG CGGCAGCAGA CACAACGGCC TCCGCGTCAT CATTCCTCCT 
CGAACCTGCG CCGCTCCCAC ACGCATCACC TGCCGCCTGG TGAAGCCCCA GAAGCTGCCC 
ACACCGCCGC CATTAGTGGA GGGAGAGGGT CTCGCCAGCC GCATCATCTC CCTTGGACCC 
GCCAGCATGC AGTTTTTAGG GCCTGTGATT GTGGAGATTC CTCATTTTGC GGCTCTCGGT 
CGGGGAGATC GGGAACTCGT GGTCTTGAGG AGTGAGAACG GATCGGTCTG GAAGGAACAT 
CGTAACCGAT ATGGAGATGA AGTGCTAGAG ACCATCCTCA ACGGCATGGA TGAAGATCTG 
GAGAGTCAGG AGGAGCTCGG GAAGAAACGC ATCAGACGGA TCATCTCCAC CGACTTCCCG 
CTCTACTTTG CGGTGGTGTC TCGCATCCAG CAGGAGAACG ATCTGATTGG CCCAGAAGGA 
GGTTTTCTGT CCAGTAAACT GGTGCCGATG GTCCAGGCCA CCTTTCCAGA GACAGCCGTC 
ACCAAACGCG TGCGTCTGGG ACTGCAGGCC CAGCCGGTGC CTGATGAGCT GGTGGCCAAG 
CTTTTGGGGA ACCAGGCCAC GTTCAGTCCT GTGGTGACGG TGGAGCCTCG GCGCCGGAAG 
TTTCACCGTC CCATCGGCCT AAGAATCCCA CTGCCACCCT CATGGAGAGA GAGCCCGCGG 
GACGCCGGAG AGGGAGACAC CACCAGCCTG CGCCTGCTCT GCAGCGTCAT CGGTGGAACA 
GCTCCTGCCC AGTGGGAAGA TATAACTGGC ACCACCAAAC TGGCGTATTC CAATGACTGT 
GCCAGTTTCA CCACCAACGT CTCTGCCCGA TTCTGGCTGG CGGACTGTCC TCGGACTGCG 
GAGGCCGTGT CCTTTGCCAA CCTCCTGTAC CGCGAGCTGT CGGCCGTGCC GTACATGGCC 
AAGTTTGTAG TGTTTGCGAA GATGAATGAA GCTCGCGAGG GTCGACTCCG CTGCTACTGC 
ATGACGGATG ACAAGATGGA CAAAACCCTG GAGCAGCACG AGAACTTCTC GGAAGTGGCT 
CGCAGCAGAG ACATCGAGGT GATGGAGGGA ATGCCGCTGT ATTTGGAGTG TTCAGGAAAC 
TTGGTTCCTA TAAGAAAAGC AGCCCAGCAG CCTCGCTGTT TCAGCTTCCA GGCCTTTAAA 
GACAACAGAC TGCCTGTGTC CGTCAAGGTG AGGGACAGCA GTAAAGACCC CTCTGGGTTT 
TTGTCTTTCT TGCGCAAGTC AACCAAATAT GAAGACAGCC AACACGTGCT GTGCAACCTC 
AACGTGACCA TGCCGCCGTG CATTAAGATT ATGGGCAGCG ATGAGCGGAG GAGGACTCTG 
ACTCCTCTGG CTCTCAGAGA AAGATACAGC GCTCTGAATG AACCGGCAAT GGCATCACTG 
AGCGCCATGG AAAGGACGGA GTTAAAGATG GCTTTAATAG CTGAACAGCT AGGCCTGAGC 
TGGGCTGAGT TGGGACGGGA ACTGCAGTTT AATGTAGATG AAATAAATAA GATCCGTGTG 
GAGAATCCCA ACTCCCTTTT AGAGCAGAGC TCAACGCTGC TCAACCTGTG GGCCGCCCGA 
GAGGGGAAGA GAGCCAAAAT GGCGTGTCTC TGTCCTCATC CTTTATGTCC AACTACCCTG 
TCTTCCCCTT CTCCATTTCT CCACCATCTG TGTGTCTCTC ACCACTCTAG TGGACAGCCT 
GTACGTGGCC CTCAAGAGCA TCGACCGAGT GGACATTGTG AACATGATGG AGGGCCAGGG 
AGGACCACCG GCGGGTCAGC AGGGGGGCTG GGAGCAGGAC GCTTCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_009302017.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009300292.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X12, mRNA.

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CloneID	ODa41289
Clone ID Related Accession (Same CDS sequence)	XM_009302017.2
Accession Version	XM_009302017.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4296bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	ankyrin 1, erythrocytic a isoform X12
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009302017.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAACCA ACACCATCCT TTTTGGAGCT GACGCTGCCA CCAGTTTCCT CCGCGCAGCT 
CGCTCAGGTA ATCTGGAAAA GGCTCTAGAC CACATCAAGA ACGGCATCGA CATCAACACA 
GCCAATCAGA ATGGACTCAA CGGGCTGCAT TTGGCCTCCA AGGAAGGCCA TGTTAAAATG 
GTGCTGGAGC TGCTTCACCA TGGTATTGTA CTTGAGACGA CAACGAAGAA AGGGAACACT 
GCTCTTCATA TAGCGGCGTT GGCTGGCCAG GAGCAGGTGG TCACTGAACT GGTCAACTAT 
GGAGCAAACG TCAACGCTCA GTCTCAGAAG GGCTTCACGC CGCTCTACAT GGCAGCACAA 
GAGAATCATC TGGAGGTTGT GAAGTTTCTG CTGGATAACG GAGCCAATCA GACCATTCCA 
ACTGAGGATG GCTTTACCCC TCTTGCTGTG GCTCTTCAGC AGGGCCATGA GAATGTGGTG 
GCCCTGCTCA TCAACCACGG TACTAAGGGC AAGGTCCGCC TTCCCGCACT GCACATCGCT 
GCCCGAAACG ACGACACCCG CACTGCCGCT GTGCTCTTAC AGAACGACCC CAATCCTGAC 
GTCCTGAGCA AGACAGGCTT CACGCCGCTC CACATCGCAG CACATTATGA AAACCTGAAT 
GTTGCTCAGC TTCTTCTGAA CCGAGGAGCT GATGTGAACT TCACACCTAA GAACGGGATC 
ACACCGCTGC ACATCGCATC CAGGAGAGGG AACGTCATCA TGGTCAGACT TCTGCTGGAC 
CGAGGAGCAA AGATCGATGC CAAGACAAAA GATGAGTTGA CTCCTCTTCA CTGTGCTGCC 
AGAAATGGCC ATGTGAGAAT CATCGAGATC CTTCTGGACC AAGGAGCCCC AATACAGGCC 
AAGACCAAAA ATGGCCTGTC GCCGATCCAC ATGGCAGCCC AGGGCGATCA CTTGGACTGT 
ATCCGACAGC TCTTACAGTA CAACGCAGAG ATCGATGACA TCACTCTGGA CCACCTGACA 
CCTCTGCATG TGGCGGCTCA CTGCGGGCAT CACCGCGTGG CCAAGGTGCT CCTGGATAAA 
GGAGCCAAAC CAAACACCCG CGCTCTGAAT GGATTCACGC CGCTCCACAT TGCCTGTAAG 
AAGAACCACA TGCGTGTAAT GGACCTGCTA TTAAAGCATT CTGCATCCCT GGAAGCTGTA 
ACCGAGTCAG GCTTGACCCC GCTGCATGTG TCCTCCTTCA TGGGTCATCT GAATATAGTC 
AAGATACTGA TGCAAAAAGG AGCTTCGCCG AACGCCTCAA ATGTGAAAGT GGAAACCCCT 
CTGCATATGG CATCTCGAGC TGGACACTGT GAAGTTGCTG AATTCCTATT GCAAAATGCA 
GCTCCAGTTG ATGCCAAAGC CAAGGATGAC CAGACGCCGC TACACTGTGC CTCCCGCATG 
GGCCACAACG AGATGGTGAA GTTGCTTCTG GAGCACAAGG CCAACTCAAA CTCCACCACC 
ACAGCAGGAC ACACACCGCT GCACATCGCG GCCCGCGAGG GACACACACA AACTGCCAGC 
ATCCTCCTGG ACATGAACGC CCAGCTCACC AAAATGACCA AGAAAGGCTT CACTCCTCTT 
CATGTTGCAG CCAAATACGG AAAGGTGGAC GTGGCAGTTC TGCTTCTGGA GAGGGGAGCA 
AATCCAAACG CTGCTGGAAA AGTTGGACTC ACGCCGCTGC ATGTGGCCGT CCATCATAAC 
AATCTAGATG TGGTCAATCT TTTACTCAGC AAAGGAGGAT CACCACACAG CGCCGCTCGG 
AATGGCTATA CTCCGCTCCA CATTGCTTCC AAACAGAACC AGGCGGAGGT GGCGAGCAGT 
TTACTGCAGC ACGGGGCGTC TGCCAATGCT GAGTCTCTGC AAGGGGTCAC ACCTCTACAC 
CTCGCCTCTC AAGAGGGTCA ACCTGACATG GTGTTACTGC TCATCTCCAA GCAGGCCAAT 
GTAAACCTTG GCAATAAGAG CGGTCTGACT CCTCTTCATC TTGTGGCTCA GGAAGGGCAT 
GTTGGGATTG CTGATATACT AGTGAAACAT GGAGCATCTG TTTATGCTGC CACACGGATG 
GGTTACACTC CGCTTCACGT GGCTTGTCAT TATGGGAATA TCAAGATGGT GAAGTTTCTC 
TTACAGCAGC AGGCCAACGT AAACTGCAAG ACCAGGATGA GCTACACGCC GCTACATCAG 
GCTGCTCAGC AGGGTCATAC GGACATCGTG ACGCTTCTGC TGAAACACGG AGCACAACCG 
AATGAAGTTA CATCTAACGG CACCTCTGCT CTTGCCATCG CCAAGCGGCT TGGATACATT 
TCAGTCATCG ATGTGCTCAA ACTGGTCACT GAAGAGACGG TCACTATGAC CACCACTGAA 
AAACATCGCA TGAGTTTCCC TGAAACCGTT GATGAGATCC TGGATGTGTC TGAAGATGAA 
GGAATTGCAC AGCTAACATT AGGCGAGGAG CTGCTGGGAA ACGAGGGCGC CAGGTATTTG 
ATGAAGATGG AGGACATAAA GGACCATGAT GAGGATTTCC TTTCCCCCAA GAAAGTTCTA 
GAGTACGAGG GAGAGTTGGA GAATCATTCT CCTGCTATCC CGAGGATCCC GTGTGTGTCT 
CCTGAGACTG TCATGCTGAA AGAGCATGAG ATGGAGCAGC AGCACACTCC ACTTCCATTG 
CCCAAAGAGT ACGACGAGGA TTCTCTGATT CCCAGCAGTC CTGCAACTGA GACTTCAGAC 
AATGTCAGTC CGGTTGCCAG TCCCATCCAC ACCGGTTTTC TGGTGAGCTT CATGGTGGAT 
GCTCGCGGCG GCTCGATGCG CGGCAGCAGA CACAACGGCC TCCGCGTCAT CATTCCTCCT 
CGAACCTGCG CCGCTCCCAC ACGCATCACC TGCCGCCTGG TGAAGCCCCA GAAGCTGCCC 
ACACCGCCGC CATTAGTGGA GGGAGAGGGT CTCGCCAGCC GCATCATCTC CCTTGGACCC 
GCCAGCATGC AGTTTTTAGG GCCTGTGATT GTGGAGATTC CTCATTTTGC GGCTCTCGGT 
CGGGGAGATC GGGAACTCGT GGTCTTGAGG AGTGAGAACG GATCGGTCTG GAAGGAACAT 
CGTAACCGAT ATGGAGATGA AGTGCTAGAG ACCATCCTCA ACGGCATGGA TGAAGATCTG 
GAGAGTCAGG AGGAGCTCGG GAAGAAACGC ATCAGACGGA TCATCTCCAC CGACTTCCCG 
CTCTACTTTG CGGTGGTGTC TCGCATCCAG CAGGAGAACG ATCTGATTGG CCCAGAAGGA 
GGTTTTCTGT CCAGTAAACT GGTGCCGATG GTCCAGGCCA CCTTTCCAGA GACAGCCGTC 
ACCAAACGCG TGCGTCTGGG ACTGCAGGCC CAGCCGGTGC CTGATGAGCT GGTGGCCAAG 
CTTTTGGGGA ACCAGGCCAC GTTCAGTCCT GTGGTGACGG TGGAGCCTCG GCGCCGGAAG 
TTTCACCGTC CCATCGGCCT AAGAATCCCA CTGCCACCCT CATGGAGAGA GAGCCCGCGG 
GACGCCGGAG AGGGAGACAC CACCAGCCTG CGCCTGCTCT GCAGCGTCAT CGGTGGAACA 
GCTCCTGCCC AGTGGGAAGA TATAACTGGC ACCACCAAAC TGGCGTATTC CAATGACTGT 
GCCAGTTTCA CCACCAACGT CTCTGCCCGA TTCTGGCTGG CGGACTGTCC TCGGACTGCG 
GAGGCCGTGT CCTTTGCCAA CCTCCTGTAC CGCGAGCTGT CGGCCGTGCC GTACATGGCC 
AAGTTTGTAG TGTTTGCGAA GATGAATGAA GCTCGCGAGG GTCGACTCCG CTGCTACTGC 
ATGACGGATG ACAAGATGGA CAAAACCCTG GAGCAGCACG AGAACTTCTC GGAAGTGGCT 
CGCAGCAGAG ACATCGAGGT GATGGAGGGA ATGCCGCTGT ATTTGGAGTG TTCAGGAAAC 
TTGGTTCCTA TAAGAAAAGC AGCCCAGCAG CCTCGCTGTT TCAGCTTCCA GGCCTTTAAA 
GACAACAGAC TGCCTGTGTC CGTCAAGGTG AGGGACAGCA GTAAAGACCC CTCTGGGTTT 
TTGTCTTTCT TGCGCAAGTC AACCAAATAT GAAGACAGCC AACACGTGCT GTGCAACCTC 
AACGTGACCA TGCCGCCGTG CATTAAGATT ATGGGCAGCG ATGAGCGGAG GAGGACTCTG 
ACTCCTCTGG CTCTCAGAGA AAGATACAGC GCTCTGAATG AACCGGCAAT GGATGTGAGC 
TGCAGCATCA GATTTGCTGA ACTCTTGAGC CAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009300292.1
CDS	335..4630
Translation	MPTNTILFGADAATSFLRAARSGNLEKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLINHGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGLTPLHVSSFMGHLNIVKILMQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGANPNAAGKVGLTPLHVAVHHNNLDVVNLLLSKGGSPHSAARNGYTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPLHLASQEGQPDMVLLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKHGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSNGTSALAIAKRLGYISVIDVLKLVTEETVTMTTTEKHRMSFPETVDEILDVSEDEGIAQLTLGEELLGNEGARYLMKMEDIKDHDEDFLSPKKVLEYEGELENHSPAIPRIPCVSPETVMLKEHEMEQQHTPLPLPKEYDEDSLIPSSPATETSDNVSPVASPIHTGFLVSFMVDARGGSMRGSRHNGLRVIIPPRTCAAPTRITCRLVKPQKLPTPPPLVEGEGLASRIISLGPASMQFLGPVIVEIPHFAALGRGDRELVVLRSENGSVWKEHRNRYGDEVLETILNGMDEDLESQEELGKKRIRRIISTDFPLYFAVVSRIQQENDLIGPEGGFLSSKLVPMVQATFPETAVTKRVRLGLQAQPVPDELVAKLLGNQATFSPVVTVEPRRRKFHRPIGLRIPLPPSWRESPRDAGEGDTTSLRLLCSVIGGTAPAQWEDITGTTKLAYSNDCASFTTNVSARFWLADCPRTAEAVSFANLLYRELSAVPYMAKFVVFAKMNEAREGRLRCYCMTDDKMDKTLEQHENFSEVARSRDIEVMEGMPLYLECSGNLVPIRKAAQQPRCFSFQAFKDNRLPVSVKVRDSSKDPSGFLSFLRKSTKYEDSQHVLCNLNVTMPPCIKIMGSDERRRTLTPLALRERYSALNEPAMDVSCSIRFAELLSQ

Target ORF information:

RefSeq Version	XM_009302017.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009302017.2

ORF Insert Sequence:

ATGCCAACCA ACACCATCCT TTTTGGAGCT GACGCTGCCA CCAGTTTCCT CCGCGCAGCT 
CGCTCAGGTA ATCTGGAAAA GGCTCTAGAC CACATCAAGA ACGGCATCGA CATCAACACA 
GCCAATCAGA ATGGACTCAA CGGGCTGCAT TTGGCCTCCA AGGAAGGCCA TGTTAAAATG 
GTGCTGGAGC TGCTTCACCA TGGTATTGTA CTTGAGACGA CAACGAAGAA AGGGAACACT 
GCTCTTCATA TAGCGGCGTT GGCTGGCCAG GAGCAGGTGG TCACTGAACT GGTCAACTAT 
GGAGCAAACG TCAACGCTCA GTCTCAGAAG GGCTTCACGC CGCTCTACAT GGCAGCACAA 
GAGAATCATC TGGAGGTTGT GAAGTTTCTG CTGGATAACG GAGCCAATCA GACCATTCCA 
ACTGAGGATG GCTTTACCCC TCTTGCTGTG GCTCTTCAGC AGGGCCATGA GAATGTGGTG 
GCCCTGCTCA TCAACCACGG TACTAAGGGC AAGGTCCGCC TTCCCGCACT GCACATCGCT 
GCCCGAAACG ACGACACCCG CACTGCCGCT GTGCTCTTAC AGAACGACCC CAATCCTGAC 
GTCCTGAGCA AGACAGGCTT CACGCCGCTC CACATCGCAG CACATTATGA AAACCTGAAT 
GTTGCTCAGC TTCTTCTGAA CCGAGGAGCT GATGTGAACT TCACACCTAA GAACGGGATC 
ACACCGCTGC ACATCGCATC CAGGAGAGGG AACGTCATCA TGGTCAGACT TCTGCTGGAC 
CGAGGAGCAA AGATCGATGC CAAGACAAAA GATGAGTTGA CTCCTCTTCA CTGTGCTGCC 
AGAAATGGCC ATGTGAGAAT CATCGAGATC CTTCTGGACC AAGGAGCCCC AATACAGGCC 
AAGACCAAAA ATGGCCTGTC GCCGATCCAC ATGGCAGCCC AGGGCGATCA CTTGGACTGT 
ATCCGACAGC TCTTACAGTA CAACGCAGAG ATCGATGACA TCACTCTGGA CCACCTGACA 
CCTCTGCATG TGGCGGCTCA CTGCGGGCAT CACCGCGTGG CCAAGGTGCT CCTGGATAAA 
GGAGCCAAAC CAAACACCCG CGCTCTGAAT GGATTCACGC CGCTCCACAT TGCCTGTAAG 
AAGAACCACA TGCGTGTAAT GGACCTGCTA TTAAAGCATT CTGCATCCCT GGAAGCTGTA 
ACCGAGTCAG GCTTGACCCC GCTGCATGTG TCCTCCTTCA TGGGTCATCT GAATATAGTC 
AAGATACTGA TGCAAAAAGG AGCTTCGCCG AACGCCTCAA ATGTGAAAGT GGAAACCCCT 
CTGCATATGG CATCTCGAGC TGGACACTGT GAAGTTGCTG AATTCCTATT GCAAAATGCA 
GCTCCAGTTG ATGCCAAAGC CAAGGATGAC CAGACGCCGC TACACTGTGC CTCCCGCATG 
GGCCACAACG AGATGGTGAA GTTGCTTCTG GAGCACAAGG CCAACTCAAA CTCCACCACC 
ACAGCAGGAC ACACACCGCT GCACATCGCG GCCCGCGAGG GACACACACA AACTGCCAGC 
ATCCTCCTGG ACATGAACGC CCAGCTCACC AAAATGACCA AGAAAGGCTT CACTCCTCTT 
CATGTTGCAG CCAAATACGG AAAGGTGGAC GTGGCAGTTC TGCTTCTGGA GAGGGGAGCA 
AATCCAAACG CTGCTGGAAA AGTTGGACTC ACGCCGCTGC ATGTGGCCGT CCATCATAAC 
AATCTAGATG TGGTCAATCT TTTACTCAGC AAAGGAGGAT CACCACACAG CGCCGCTCGG 
AATGGCTATA CTCCGCTCCA CATTGCTTCC AAACAGAACC AGGCGGAGGT GGCGAGCAGT 
TTACTGCAGC ACGGGGCGTC TGCCAATGCT GAGTCTCTGC AAGGGGTCAC ACCTCTACAC 
CTCGCCTCTC AAGAGGGTCA ACCTGACATG GTGTTACTGC TCATCTCCAA GCAGGCCAAT 
GTAAACCTTG GCAATAAGAG CGGTCTGACT CCTCTTCATC TTGTGGCTCA GGAAGGGCAT 
GTTGGGATTG CTGATATACT AGTGAAACAT GGAGCATCTG TTTATGCTGC CACACGGATG 
GGTTACACTC CGCTTCACGT GGCTTGTCAT TATGGGAATA TCAAGATGGT GAAGTTTCTC 
TTACAGCAGC AGGCCAACGT AAACTGCAAG ACCAGGATGA GCTACACGCC GCTACATCAG 
GCTGCTCAGC AGGGTCATAC GGACATCGTG ACGCTTCTGC TGAAACACGG AGCACAACCG 
AATGAAGTTA CATCTAACGG CACCTCTGCT CTTGCCATCG CCAAGCGGCT TGGATACATT 
TCAGTCATCG ATGTGCTCAA ACTGGTCACT GAAGAGACGG TCACTATGAC CACCACTGAA 
AAACATCGCA TGAGTTTCCC TGAAACCGTT GATGAGATCC TGGATGTGTC TGAAGATGAA 
GGAATTGCAC AGCTAACATT AGGCGAGGAG CTGCTGGGAA ACGAGGGCGC CAGGTATTTG 
ATGAAGATGG AGGACATAAA GGACCATGAT GAGGATTTCC TTTCCCCCAA GAAAGTTCTA 
GAGTACGAGG GAGAGTTGGA GAATCATTCT CCTGCTATCC CGAGGATCCC GTGTGTGTCT 
CCTGAGACTG TCATGCTGAA AGAGCATGAG ATGGAGCAGC AGCACACTCC ACTTCCATTG 
CCCAAAGAGT ACGACGAGGA TTCTCTGATT CCCAGCAGTC CTGCAACTGA GACTTCAGAC 
AATGTCAGTC CGGTTGCCAG TCCCATCCAC ACCGGTTTTC TGGTGAGCTT CATGGTGGAT 
GCTCGCGGCG GCTCGATGCG CGGCAGCAGA CACAACGGCC TCCGCGTCAT CATTCCTCCT 
CGAACCTGCG CCGCTCCCAC ACGCATCACC TGCCGCCTGG TGAAGCCCCA GAAGCTGCCC 
ACACCGCCGC CATTAGTGGA GGGAGAGGGT CTCGCCAGCC GCATCATCTC CCTTGGACCC 
GCCAGCATGC AGTTTTTAGG GCCTGTGATT GTGGAGATTC CTCATTTTGC GGCTCTCGGT 
CGGGGAGATC GGGAACTCGT GGTCTTGAGG AGTGAGAACG GATCGGTCTG GAAGGAACAT 
CGTAACCGAT ATGGAGATGA AGTGCTAGAG ACCATCCTCA ACGGCATGGA TGAAGATCTG 
GAGAGTCAGG AGGAGCTCGG GAAGAAACGC ATCAGACGGA TCATCTCCAC CGACTTCCCG 
CTCTACTTTG CGGTGGTGTC TCGCATCCAG CAGGAGAACG ATCTGATTGG CCCAGAAGGA 
GGTTTTCTGT CCAGTAAACT GGTGCCGATG GTCCAGGCCA CCTTTCCAGA GACAGCCGTC 
ACCAAACGCG TGCGTCTGGG ACTGCAGGCC CAGCCGGTGC CTGATGAGCT GGTGGCCAAG 
CTTTTGGGGA ACCAGGCCAC GTTCAGTCCT GTGGTGACGG TGGAGCCTCG GCGCCGGAAG 
TTTCACCGTC CCATCGGCCT AAGAATCCCA CTGCCACCCT CATGGAGAGA GAGCCCGCGG 
GACGCCGGAG AGGGAGACAC CACCAGCCTG CGCCTGCTCT GCAGCGTCAT CGGTGGAACA 
GCTCCTGCCC AGTGGGAAGA TATAACTGGC ACCACCAAAC TGGCGTATTC CAATGACTGT 
GCCAGTTTCA CCACCAACGT CTCTGCCCGA TTCTGGCTGG CGGACTGTCC TCGGACTGCG 
GAGGCCGTGT CCTTTGCCAA CCTCCTGTAC CGCGAGCTGT CGGCCGTGCC GTACATGGCC 
AAGTTTGTAG TGTTTGCGAA GATGAATGAA GCTCGCGAGG GTCGACTCCG CTGCTACTGC 
ATGACGGATG ACAAGATGGA CAAAACCCTG GAGCAGCACG AGAACTTCTC GGAAGTGGCT 
CGCAGCAGAG ACATCGAGGT GATGGAGGGA ATGCCGCTGT ATTTGGAGTG TTCAGGAAAC 
TTGGTTCCTA TAAGAAAAGC AGCCCAGCAG CCTCGCTGTT TCAGCTTCCA GGCCTTTAAA 
GACAACAGAC TGCCTGTGTC CGTCAAGGTG AGGGACAGCA GTAAAGACCC CTCTGGGTTT 
TTGTCTTTCT TGCGCAAGTC AACCAAATAT GAAGACAGCC AACACGTGCT GTGCAACCTC 
AACGTGACCA TGCCGCCGTG CATTAAGATT ATGGGCAGCG ATGAGCGGAG GAGGACTCTG 
ACTCCTCTGG CTCTCAGAGA AAGATACAGC GCTCTGAATG AACCGGCAAT GGATGTGAGC 
TGCAGCATCA GATTTGCTGA ACTCTTGAGC CAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_021476099.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331774.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X1, mRNA.

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CloneID	ODa142591
Clone ID Related Accession (Same CDS sequence)	XM_021476099.1
Accession Version	XM_021476099.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6102bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin 1, erythrocytic a isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGAATTGC ACAGCTAACA TTAGGCGAGG AGCTGCTGGG AAACGAGGGC 
GCCAGGTATT TGATGAAGAT GGAGGACATA AAGGACCATG ATGAGGATTT CCTTTCCCCC 
AAGAAAGTTC TAGAGTACGA GGGAGAGTTG GAGAATCATT CTCCTGCTAT CCCGAGGATC 
CCGTGTGTGT CTCCTGAGAC TGTCATGCTG AAAGAGCATG AGATGGAGCA GCAGCACACT 
CCACTTCCAT TGCCCAAAGA GTACGACGAG GATTCTCTGA TTCCCAGCAG TCCTGCAACT 
GAGACTTCAG ACAATGTCAG TCCGGTTGCC AGTCCCATCC ACACCGGTTT TCTGGTGAGC 
TTCATGGTGG ATGCTCGCGG CGGCTCGATG CGCGGCAGCA GACACAACGG CCTCCGCGTC 
ATCATTCCTC CTCGAACCTG CGCCGCTCCC ACACGCATCA CCTGCCGCCT GGTGAAGCCC 
CAGAAGCTGC CCACACCGCC GCCATTAGTG GAGGGAGAGG GTCTCGCCAG CCGCATCATC 
TCCCTTGGAC CCGCCAGCAT GCAGTTTTTA GGGCCTGTGA TTGTGGAGAT TCCTCATTTT 
GCGGCTCTCG GTCGGGGAGA TCGGGAACTC GTGGTCTTGA GGAGTGAGAA CGGATCGGTC 
TGGAAGGAAC ATCGTAACCG ATATGGAGAT GAAGTGCTAG AGACCATCCT CAACGGCATG 
GATGAAGATC TGGAGAGTCA GGAGGAGCTC GGGAAGAAAC GCATCAGACG GATCATCTCC 
ACCGACTTCC CGCTCTACTT TGCGGTGGTG TCTCGCATCC AGCAGGAGAA CGATCTGATT 
GGCCCAGAAG GAGGTTTTCT GTCCAGTAAA CTGGTGCCGA TGGTCCAGGC CACCTTTCCA 
GAGACAGCCG TCACCAAACG CGTGCGTCTG GGACTGCAGG CCCAGCCGGT GCCTGATGAG 
CTGGTGGCCA AGCTTTTGGG GAACCAGGCC ACGTTCAGTC CTGTGGTGAC GGTGGAGCCT 
CGGCGCCGGA AGTTTCACCG TCCCATCGGC CTAAGAATCC CACTGCCACC CTCATGGAGA 
GAGAGCCCGC GGGACGCCGG AGAGGGAGAC ACCACCAGCC TGCGCCTGCT CTGCAGCGTC 
ATCGGTGGAA CAGCTCCTGC CCAGTGGGAA GATATAACTG GCACCACCAA ACTGGCGTAT 
TCCAATGACT GTGCCAGTTT CACCACCAAC GTCTCTGCCC GATTCTGGCT GGCGGACTGT 
CCTCGGACTG CGGAGGCCGT GTCCTTTGCC AACCTCCTGT ACCGCGAGCT GTCGGCCGTG 
CCGTACATGG CCAAGTTTGT AGTGTTTGCG AAGATGAATG AAGCTCGCGA GGGTCGACTC 
CGCTGCTACT GCATGACGGA TGACAAGATG GACAAAACCC TGGAGCAGCA CGAGAACTTC 
TCGGAAGTGG CTCGCAGCAG AGACATCGAG GTGATGGAGG GAATGCCGCT GTATTTGGAG 
TGTTCAGGAA ACTTGGTTCC TATAAGAAAA GCAGCCCAGC AGCCTCGCTG TTTCAGCTTC 
CAGGCCTTTA AAGACAACAG ACTGCCTGTG TCCGTCAAGG TGAGGGACAG CAGTAAAGAC 
CCCTCTGGGT TTTTGTCTTT CTTGCGCAAG TCAACCAAAT ATGAAGACAG CCAACACGTG 
CTGTGCAACC TCAACGTGAC CATGCCGCCG TGCATTAAGA TTATGGGCAG CGATGAGCGG 
AGGAGGACTC TGACTCCTCT GGCTCTCAGA GAAAGATACA GCGCTCTGAA TGAACCGGCA 
ATGGCATCAC TGAGCGCCAT GGAAAGGACG GAGTTAAAGA TGGCTTTAAT AGCTGAACAG 
CTAGGCCTGA GCTGGGCTGA GTTGGGACGG GAACTGCAGT TTAATGTAGA TGAAATAAAT 
AAGATCCGTG TGGAGAATCC CAACTCCCTT TTAGAGCAGA GCTCAACGCT GCTCAACCTG 
TGGGCCGCCC GAGAGGGGAA GAGAGCCAAA ATGGACAGCC TGTACGTGGC CCTCAAGAGC 
ATCGACCGAG TGGACATTGT GAACATGATG GAGGGCCAGG GAGGACCACC GGCGGGTCAG 
CAGGGGGGCT GGGAGCAGGA CGCTTCTAGG TCTAGGCATC ACGACCGAGA CCATCTCTCA 
CCTGGAATCA CAAATGGTTA CGGGCTGGTG GTGCAGGAGG AACTAGTGTC ACCGGCCTCC 
ATGCAGTACA GTCTGCCCTC GCCACTGGGC AATGAGCCCT ACTGGCAGGA GGTTTCCAGC 
ATGGAGTGCG CGCCCATCGC CACCACTGAA GAAGACACGC TCATGGAGAT GTCCGAGGTA 
CAGGTTTGGC CCTCAGGAAA TAGTCCGTCC ATTGTGGCTG TAGAGGACTC CTCATTGGAG 
TGCAGCAATG CGGACGACTC CGAGGCGCTG CAGAACCGGA GCCTGGGTCA CTCCAGGAGC 
GGGACGCCCG GTGGGAACGG ACTCACTCCG GGAGGTTTAA GTGGCTCCAT CGAGCTCCTG 
GAGGATCAGT CCATCGGTGC GGATTCAGAC TACAGTGCCG CCGGGATGCT GCGTCTGAGC 
GAAGGGGTCA ACATCAGTGC TCTGGACAGA GGTCAGAGGT CGGAGAGTTC AGAAGGGGCT 
GTAGGTCACC AAGTCACCAA CAACATTAAA GACGGTTCCA ATGGAGGTTT GGGAGAAGGA 
GGAGGTGACG GCACACAGTT AGAAGATAGC ATATCTCTCA TTTCAGGGCA ACAGCGTGTG 
TATGCTCGAA TGAGCGAGTC GCCTGGGCTG AGAAGACGGG CAGATCCTGG CGAAGACAGG 
TTAAGCGCAG GGTCGTTCCT GTCTTATCTG CAGGATCAGG GCTCTCCTGG TTGGCATTCG 
GCTGCAGAAG CGACTCAGAA CAGATTGGCG TCTTTGCAAT GCTCCCTTCC TAGGCAGGCA 
ATAGAGTCTA TGATGTCATC GGTGCGAGCT ACGGTGGACT CTGACTCCAA GCAGTCTTGT 
TTGGGCCAGG AGGCTCTGAT TGAGCCAGTG CGGGACATGG GGCACTCTGA GATCATCCAT 
GGCCATTATC AGGGGACTCA GCCGTTTGAG AAAGGACTCG GCTTCCCGCA CAGAACAACC 
AACATGCGCA CTTGGGAGGA CATGCGTCTG AGCGGACAGG GTGATGAGGC TGAGGATCTT 
CCAGGAGAGC AAGTGAGCGA GGAGCAATTC ACAGACGAGC ATGGCAACAT CGTCACCAAA 
AAGATTGTGA GGAAAGTGGT CCGGAGAGGA AAAGGGGAGG AAGGGGGTCA GGAGCTGATC 
ATTGAAGGTC TGCAGCAGGA TATCAGTGAG CCTGACTTTG ATGGAGAACA GTTCATGAGC 
TATGCCGTCC TGGGACGTGA CAGCAAGCCG GATGTTGTGG ATGTGAAGAG AGGAGGTGCT 
CAGATAGTCA AATGTGCGAG TCTGCGGAGA GTAAAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331774.1
CDS	222..6323
Translation	MAQAAKHLRKNKDLEALAEQERKEKEEEKSKKRSRSREKKRKAHVVHRWLIDQDNSVSSELPDGQGVWHYDETADAATSFLRAARSGNLEKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLINHGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGLTPLHVSSFMGHLNIVKILMQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGANPNAAGKVGLTPLHVAVHHNNLDVVNLLLSKGGSPHSAARNGYTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPLHLASQEGQPDMVLLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKHGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSNGTSALAIAKRLGYISVIDVLKLVTEETVTMTTTEKHRMSFPETVDEILDVSEDEGIAQLTLGEELLGNEGARYLMKMEDIKDHDEDFLSPKKVLEYEGELENHSPAIPRIPCVSPETVMLKEHEMEQQHTPLPLPKEYDEDSLIPSSPATETSDNVSPVASPIHTGFLVSFMVDARGGSMRGSRHNGLRVIIPPRTCAAPTRITCRLVKPQKLPTPPPLVEGEGLASRIISLGPASMQFLGPVIVEIPHFAALGRGDRELVVLRSENGSVWKEHRNRYGDEVLETILNGMDEDLESQEELGKKRIRRIISTDFPLYFAVVSRIQQENDLIGPEGGFLSSKLVPMVQATFPETAVTKRVRLGLQAQPVPDELVAKLLGNQATFSPVVTVEPRRRKFHRPIGLRIPLPPSWRESPRDAGEGDTTSLRLLCSVIGGTAPAQWEDITGTTKLAYSNDCASFTTNVSARFWLADCPRTAEAVSFANLLYRELSAVPYMAKFVVFAKMNEAREGRLRCYCMTDDKMDKTLEQHENFSEVARSRDIEVMEGMPLYLECSGNLVPIRKAAQQPRCFSFQAFKDNRLPVSVKVRDSSKDPSGFLSFLRKSTKYEDSQHVLCNLNVTMPPCIKIMGSDERRRTLTPLALRERYSALNEPAMASLSAMERTELKMALIAEQLGLSWAELGRELQFNVDEINKIRVENPNSLLEQSSTLLNLWAAREGKRAKMDSLYVALKSIDRVDIVNMMEGQGGPPAGQQGGWEQDASRSRHHDRDHLSPGITNGYGLVVQEELVSPASMQYSLPSPLGNEPYWQEVSSMECAPIATTEEDTLMEMSEVQVWPSGNSPSIVAVEDSSLECSNADDSEALQNRSLGHSRSGTPGGNGLTPGGLSGSIELLEDQSIGADSDYSAAGMLRLSEGVNISALDRGQRSESSEGAVGHQVTNNIKDGSNGGLGEGGGDGTQLEDSISLISGQQRVYARMSESPGLRRRADPGEDRLSAGSFLSYLQDQGSPGWHSAAEATQNRLASLQCSLPRQAIESMMSSVRATVDSDSKQSCLGQEALIEPVRDMGHSEIIHGHYQGTQPFEKGLGFPHRTTNMRTWEDMRLSGQGDEAEDLPGEQVSEEQFTDEHGNIVTKKIVRKVVRRGKGEEGGQELIIEGLQQDISEPDFDGEQFMSYAVLGRDSKPDVVDVKRGGAQIVKCASLRRVKQ

Target ORF information:

RefSeq Version	XM_021476099.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476099.1

ORF Insert Sequence:

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGAATTGC ACAGCTAACA TTAGGCGAGG AGCTGCTGGG AAACGAGGGC 
GCCAGGTATT TGATGAAGAT GGAGGACATA AAGGACCATG ATGAGGATTT CCTTTCCCCC 
AAGAAAGTTC TAGAGTACGA GGGAGAGTTG GAGAATCATT CTCCTGCTAT CCCGAGGATC 
CCGTGTGTGT CTCCTGAGAC TGTCATGCTG AAAGAGCATG AGATGGAGCA GCAGCACACT 
CCACTTCCAT TGCCCAAAGA GTACGACGAG GATTCTCTGA TTCCCAGCAG TCCTGCAACT 
GAGACTTCAG ACAATGTCAG TCCGGTTGCC AGTCCCATCC ACACCGGTTT TCTGGTGAGC 
TTCATGGTGG ATGCTCGCGG CGGCTCGATG CGCGGCAGCA GACACAACGG CCTCCGCGTC 
ATCATTCCTC CTCGAACCTG CGCCGCTCCC ACACGCATCA CCTGCCGCCT GGTGAAGCCC 
CAGAAGCTGC CCACACCGCC GCCATTAGTG GAGGGAGAGG GTCTCGCCAG CCGCATCATC 
TCCCTTGGAC CCGCCAGCAT GCAGTTTTTA GGGCCTGTGA TTGTGGAGAT TCCTCATTTT 
GCGGCTCTCG GTCGGGGAGA TCGGGAACTC GTGGTCTTGA GGAGTGAGAA CGGATCGGTC 
TGGAAGGAAC ATCGTAACCG ATATGGAGAT GAAGTGCTAG AGACCATCCT CAACGGCATG 
GATGAAGATC TGGAGAGTCA GGAGGAGCTC GGGAAGAAAC GCATCAGACG GATCATCTCC 
ACCGACTTCC CGCTCTACTT TGCGGTGGTG TCTCGCATCC AGCAGGAGAA CGATCTGATT 
GGCCCAGAAG GAGGTTTTCT GTCCAGTAAA CTGGTGCCGA TGGTCCAGGC CACCTTTCCA 
GAGACAGCCG TCACCAAACG CGTGCGTCTG GGACTGCAGG CCCAGCCGGT GCCTGATGAG 
CTGGTGGCCA AGCTTTTGGG GAACCAGGCC ACGTTCAGTC CTGTGGTGAC GGTGGAGCCT 
CGGCGCCGGA AGTTTCACCG TCCCATCGGC CTAAGAATCC CACTGCCACC CTCATGGAGA 
GAGAGCCCGC GGGACGCCGG AGAGGGAGAC ACCACCAGCC TGCGCCTGCT CTGCAGCGTC 
ATCGGTGGAA CAGCTCCTGC CCAGTGGGAA GATATAACTG GCACCACCAA ACTGGCGTAT 
TCCAATGACT GTGCCAGTTT CACCACCAAC GTCTCTGCCC GATTCTGGCT GGCGGACTGT 
CCTCGGACTG CGGAGGCCGT GTCCTTTGCC AACCTCCTGT ACCGCGAGCT GTCGGCCGTG 
CCGTACATGG CCAAGTTTGT AGTGTTTGCG AAGATGAATG AAGCTCGCGA GGGTCGACTC 
CGCTGCTACT GCATGACGGA TGACAAGATG GACAAAACCC TGGAGCAGCA CGAGAACTTC 
TCGGAAGTGG CTCGCAGCAG AGACATCGAG GTGATGGAGG GAATGCCGCT GTATTTGGAG 
TGTTCAGGAA ACTTGGTTCC TATAAGAAAA GCAGCCCAGC AGCCTCGCTG TTTCAGCTTC 
CAGGCCTTTA AAGACAACAG ACTGCCTGTG TCCGTCAAGG TGAGGGACAG CAGTAAAGAC 
CCCTCTGGGT TTTTGTCTTT CTTGCGCAAG TCAACCAAAT ATGAAGACAG CCAACACGTG 
CTGTGCAACC TCAACGTGAC CATGCCGCCG TGCATTAAGA TTATGGGCAG CGATGAGCGG 
AGGAGGACTC TGACTCCTCT GGCTCTCAGA GAAAGATACA GCGCTCTGAA TGAACCGGCA 
ATGGCATCAC TGAGCGCCAT GGAAAGGACG GAGTTAAAGA TGGCTTTAAT AGCTGAACAG 
CTAGGCCTGA GCTGGGCTGA GTTGGGACGG GAACTGCAGT TTAATGTAGA TGAAATAAAT 
AAGATCCGTG TGGAGAATCC CAACTCCCTT TTAGAGCAGA GCTCAACGCT GCTCAACCTG 
TGGGCCGCCC GAGAGGGGAA GAGAGCCAAA ATGGACAGCC TGTACGTGGC CCTCAAGAGC 
ATCGACCGAG TGGACATTGT GAACATGATG GAGGGCCAGG GAGGACCACC GGCGGGTCAG 
CAGGGGGGCT GGGAGCAGGA CGCTTCTAGG TCTAGGCATC ACGACCGAGA CCATCTCTCA 
CCTGGAATCA CAAATGGTTA CGGGCTGGTG GTGCAGGAGG AACTAGTGTC ACCGGCCTCC 
ATGCAGTACA GTCTGCCCTC GCCACTGGGC AATGAGCCCT ACTGGCAGGA GGTTTCCAGC 
ATGGAGTGCG CGCCCATCGC CACCACTGAA GAAGACACGC TCATGGAGAT GTCCGAGGTA 
CAGGTTTGGC CCTCAGGAAA TAGTCCGTCC ATTGTGGCTG TAGAGGACTC CTCATTGGAG 
TGCAGCAATG CGGACGACTC CGAGGCGCTG CAGAACCGGA GCCTGGGTCA CTCCAGGAGC 
GGGACGCCCG GTGGGAACGG ACTCACTCCG GGAGGTTTAA GTGGCTCCAT CGAGCTCCTG 
GAGGATCAGT CCATCGGTGC GGATTCAGAC TACAGTGCCG CCGGGATGCT GCGTCTGAGC 
GAAGGGGTCA ACATCAGTGC TCTGGACAGA GGTCAGAGGT CGGAGAGTTC AGAAGGGGCT 
GTAGGTCACC AAGTCACCAA CAACATTAAA GACGGTTCCA ATGGAGGTTT GGGAGAAGGA 
GGAGGTGACG GCACACAGTT AGAAGATAGC ATATCTCTCA TTTCAGGGCA ACAGCGTGTG 
TATGCTCGAA TGAGCGAGTC GCCTGGGCTG AGAAGACGGG CAGATCCTGG CGAAGACAGG 
TTAAGCGCAG GGTCGTTCCT GTCTTATCTG CAGGATCAGG GCTCTCCTGG TTGGCATTCG 
GCTGCAGAAG CGACTCAGAA CAGATTGGCG TCTTTGCAAT GCTCCCTTCC TAGGCAGGCA 
ATAGAGTCTA TGATGTCATC GGTGCGAGCT ACGGTGGACT CTGACTCCAA GCAGTCTTGT 
TTGGGCCAGG AGGCTCTGAT TGAGCCAGTG CGGGACATGG GGCACTCTGA GATCATCCAT 
GGCCATTATC AGGGGACTCA GCCGTTTGAG AAAGGACTCG GCTTCCCGCA CAGAACAACC 
AACATGCGCA CTTGGGAGGA CATGCGTCTG AGCGGACAGG GTGATGAGGC TGAGGATCTT 
CCAGGAGAGC AAGTGAGCGA GGAGCAATTC ACAGACGAGC ATGGCAACAT CGTCACCAAA 
AAGATTGTGA GGAAAGTGGT CCGGAGAGGA AAAGGGGAGG AAGGGGGTCA GGAGCTGATC 
ATTGAAGGTC TGCAGCAGGA TATCAGTGAG CCTGACTTTG ATGGAGAACA GTTCATGAGC 
TATGCCGTCC TGGGACGTGA CAGCAAGCCG GATGTTGTGG ATGTGAAGAG AGGAGGTGCT 
CAGATAGTCA AATGTGCGAG TCTGCGGAGA GTAAAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_021476100.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331775.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X2, mRNA.

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CloneID	ODa142592
Clone ID Related Accession (Same CDS sequence)	XM_021476100.1
Accession Version	XM_021476100.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6099bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin 1, erythrocytic a isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGAATTGC ACAGCTAACA TTAGGCGAGG AGCTGCTGGG AAACGAGGGC 
GCCAGGTATT TGATGAAGAT GGAGGACATA AAGGACCATG ATGAGGATTT CCTTTCCCCC 
AAGAAAGTTC TAGAGTACGA GGGAGAGTTG GAGAATCATT CTCCTGCTAT CCCGAGGATC 
CCGTGTGTGT CTCCTGAGAC TGTCATGCTG AAAGAGCATG AGATGGAGCA GCACACTCCA 
CTTCCATTGC CCAAAGAGTA CGACGAGGAT TCTCTGATTC CCAGCAGTCC TGCAACTGAG 
ACTTCAGACA ATGTCAGTCC GGTTGCCAGT CCCATCCACA CCGGTTTTCT GGTGAGCTTC 
ATGGTGGATG CTCGCGGCGG CTCGATGCGC GGCAGCAGAC ACAACGGCCT CCGCGTCATC 
ATTCCTCCTC GAACCTGCGC CGCTCCCACA CGCATCACCT GCCGCCTGGT GAAGCCCCAG 
AAGCTGCCCA CACCGCCGCC ATTAGTGGAG GGAGAGGGTC TCGCCAGCCG CATCATCTCC 
CTTGGACCCG CCAGCATGCA GTTTTTAGGG CCTGTGATTG TGGAGATTCC TCATTTTGCG 
GCTCTCGGTC GGGGAGATCG GGAACTCGTG GTCTTGAGGA GTGAGAACGG ATCGGTCTGG 
AAGGAACATC GTAACCGATA TGGAGATGAA GTGCTAGAGA CCATCCTCAA CGGCATGGAT 
GAAGATCTGG AGAGTCAGGA GGAGCTCGGG AAGAAACGCA TCAGACGGAT CATCTCCACC 
GACTTCCCGC TCTACTTTGC GGTGGTGTCT CGCATCCAGC AGGAGAACGA TCTGATTGGC 
CCAGAAGGAG GTTTTCTGTC CAGTAAACTG GTGCCGATGG TCCAGGCCAC CTTTCCAGAG 
ACAGCCGTCA CCAAACGCGT GCGTCTGGGA CTGCAGGCCC AGCCGGTGCC TGATGAGCTG 
GTGGCCAAGC TTTTGGGGAA CCAGGCCACG TTCAGTCCTG TGGTGACGGT GGAGCCTCGG 
CGCCGGAAGT TTCACCGTCC CATCGGCCTA AGAATCCCAC TGCCACCCTC ATGGAGAGAG 
AGCCCGCGGG ACGCCGGAGA GGGAGACACC ACCAGCCTGC GCCTGCTCTG CAGCGTCATC 
GGTGGAACAG CTCCTGCCCA GTGGGAAGAT ATAACTGGCA CCACCAAACT GGCGTATTCC 
AATGACTGTG CCAGTTTCAC CACCAACGTC TCTGCCCGAT TCTGGCTGGC GGACTGTCCT 
CGGACTGCGG AGGCCGTGTC CTTTGCCAAC CTCCTGTACC GCGAGCTGTC GGCCGTGCCG 
TACATGGCCA AGTTTGTAGT GTTTGCGAAG ATGAATGAAG CTCGCGAGGG TCGACTCCGC 
TGCTACTGCA TGACGGATGA CAAGATGGAC AAAACCCTGG AGCAGCACGA GAACTTCTCG 
GAAGTGGCTC GCAGCAGAGA CATCGAGGTG ATGGAGGGAA TGCCGCTGTA TTTGGAGTGT 
TCAGGAAACT TGGTTCCTAT AAGAAAAGCA GCCCAGCAGC CTCGCTGTTT CAGCTTCCAG 
GCCTTTAAAG ACAACAGACT GCCTGTGTCC GTCAAGGTGA GGGACAGCAG TAAAGACCCC 
TCTGGGTTTT TGTCTTTCTT GCGCAAGTCA ACCAAATATG AAGACAGCCA ACACGTGCTG 
TGCAACCTCA ACGTGACCAT GCCGCCGTGC ATTAAGATTA TGGGCAGCGA TGAGCGGAGG 
AGGACTCTGA CTCCTCTGGC TCTCAGAGAA AGATACAGCG CTCTGAATGA ACCGGCAATG 
GCATCACTGA GCGCCATGGA AAGGACGGAG TTAAAGATGG CTTTAATAGC TGAACAGCTA 
GGCCTGAGCT GGGCTGAGTT GGGACGGGAA CTGCAGTTTA ATGTAGATGA AATAAATAAG 
ATCCGTGTGG AGAATCCCAA CTCCCTTTTA GAGCAGAGCT CAACGCTGCT CAACCTGTGG 
GCCGCCCGAG AGGGGAAGAG AGCCAAAATG GACAGCCTGT ACGTGGCCCT CAAGAGCATC 
GACCGAGTGG ACATTGTGAA CATGATGGAG GGCCAGGGAG GACCACCGGC GGGTCAGCAG 
GGGGGCTGGG AGCAGGACGC TTCTAGGTCT AGGCATCACG ACCGAGACCA TCTCTCACCT 
GGAATCACAA ATGGTTACGG GCTGGTGGTG CAGGAGGAAC TAGTGTCACC GGCCTCCATG 
CAGTACAGTC TGCCCTCGCC ACTGGGCAAT GAGCCCTACT GGCAGGAGGT TTCCAGCATG 
GAGTGCGCGC CCATCGCCAC CACTGAAGAA GACACGCTCA TGGAGATGTC CGAGGTACAG 
GTTTGGCCCT CAGGAAATAG TCCGTCCATT GTGGCTGTAG AGGACTCCTC ATTGGAGTGC 
AGCAATGCGG ACGACTCCGA GGCGCTGCAG AACCGGAGCC TGGGTCACTC CAGGAGCGGG 
ACGCCCGGTG GGAACGGACT CACTCCGGGA GGTTTAAGTG GCTCCATCGA GCTCCTGGAG 
GATCAGTCCA TCGGTGCGGA TTCAGACTAC AGTGCCGCCG GGATGCTGCG TCTGAGCGAA 
GGGGTCAACA TCAGTGCTCT GGACAGAGGT CAGAGGTCGG AGAGTTCAGA AGGGGCTGTA 
GGTCACCAAG TCACCAACAA CATTAAAGAC GGTTCCAATG GAGGTTTGGG AGAAGGAGGA 
GGTGACGGCA CACAGTTAGA AGATAGCATA TCTCTCATTT CAGGGCAACA GCGTGTGTAT 
GCTCGAATGA GCGAGTCGCC TGGGCTGAGA AGACGGGCAG ATCCTGGCGA AGACAGGTTA 
AGCGCAGGGT CGTTCCTGTC TTATCTGCAG GATCAGGGCT CTCCTGGTTG GCATTCGGCT 
GCAGAAGCGA CTCAGAACAG ATTGGCGTCT TTGCAATGCT CCCTTCCTAG GCAGGCAATA 
GAGTCTATGA TGTCATCGGT GCGAGCTACG GTGGACTCTG ACTCCAAGCA GTCTTGTTTG 
GGCCAGGAGG CTCTGATTGA GCCAGTGCGG GACATGGGGC ACTCTGAGAT CATCCATGGC 
CATTATCAGG GGACTCAGCC GTTTGAGAAA GGACTCGGCT TCCCGCACAG AACAACCAAC 
ATGCGCACTT GGGAGGACAT GCGTCTGAGC GGACAGGGTG ATGAGGCTGA GGATCTTCCA 
GGAGAGCAAG TGAGCGAGGA GCAATTCACA GACGAGCATG GCAACATCGT CACCAAAAAG 
ATTGTGAGGA AAGTGGTCCG GAGAGGAAAA GGGGAGGAAG GGGGTCAGGA GCTGATCATT 
GAAGGTCTGC AGCAGGATAT CAGTGAGCCT GACTTTGATG GAGAACAGTT CATGAGCTAT 
GCCGTCCTGG GACGTGACAG CAAGCCGGAT GTTGTGGATG TGAAGAGAGG AGGTGCTCAG 
ATAGTCAAAT GTGCGAGTCT GCGGAGAGTA AAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331775.1
CDS	222..6320
Translation	MAQAAKHLRKNKDLEALAEQERKEKEEEKSKKRSRSREKKRKAHVVHRWLIDQDNSVSSELPDGQGVWHYDETADAATSFLRAARSGNLEKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLINHGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGLTPLHVSSFMGHLNIVKILMQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGANPNAAGKVGLTPLHVAVHHNNLDVVNLLLSKGGSPHSAARNGYTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPLHLASQEGQPDMVLLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKHGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSNGTSALAIAKRLGYISVIDVLKLVTEETVTMTTTEKHRMSFPETVDEILDVSEDEGIAQLTLGEELLGNEGARYLMKMEDIKDHDEDFLSPKKVLEYEGELENHSPAIPRIPCVSPETVMLKEHEMEQHTPLPLPKEYDEDSLIPSSPATETSDNVSPVASPIHTGFLVSFMVDARGGSMRGSRHNGLRVIIPPRTCAAPTRITCRLVKPQKLPTPPPLVEGEGLASRIISLGPASMQFLGPVIVEIPHFAALGRGDRELVVLRSENGSVWKEHRNRYGDEVLETILNGMDEDLESQEELGKKRIRRIISTDFPLYFAVVSRIQQENDLIGPEGGFLSSKLVPMVQATFPETAVTKRVRLGLQAQPVPDELVAKLLGNQATFSPVVTVEPRRRKFHRPIGLRIPLPPSWRESPRDAGEGDTTSLRLLCSVIGGTAPAQWEDITGTTKLAYSNDCASFTTNVSARFWLADCPRTAEAVSFANLLYRELSAVPYMAKFVVFAKMNEAREGRLRCYCMTDDKMDKTLEQHENFSEVARSRDIEVMEGMPLYLECSGNLVPIRKAAQQPRCFSFQAFKDNRLPVSVKVRDSSKDPSGFLSFLRKSTKYEDSQHVLCNLNVTMPPCIKIMGSDERRRTLTPLALRERYSALNEPAMASLSAMERTELKMALIAEQLGLSWAELGRELQFNVDEINKIRVENPNSLLEQSSTLLNLWAAREGKRAKMDSLYVALKSIDRVDIVNMMEGQGGPPAGQQGGWEQDASRSRHHDRDHLSPGITNGYGLVVQEELVSPASMQYSLPSPLGNEPYWQEVSSMECAPIATTEEDTLMEMSEVQVWPSGNSPSIVAVEDSSLECSNADDSEALQNRSLGHSRSGTPGGNGLTPGGLSGSIELLEDQSIGADSDYSAAGMLRLSEGVNISALDRGQRSESSEGAVGHQVTNNIKDGSNGGLGEGGGDGTQLEDSISLISGQQRVYARMSESPGLRRRADPGEDRLSAGSFLSYLQDQGSPGWHSAAEATQNRLASLQCSLPRQAIESMMSSVRATVDSDSKQSCLGQEALIEPVRDMGHSEIIHGHYQGTQPFEKGLGFPHRTTNMRTWEDMRLSGQGDEAEDLPGEQVSEEQFTDEHGNIVTKKIVRKVVRRGKGEEGGQELIIEGLQQDISEPDFDGEQFMSYAVLGRDSKPDVVDVKRGGAQIVKCASLRRVKQ

Target ORF information:

RefSeq Version	XM_021476100.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476100.1

ORF Insert Sequence:

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGAATTGC ACAGCTAACA TTAGGCGAGG AGCTGCTGGG AAACGAGGGC 
GCCAGGTATT TGATGAAGAT GGAGGACATA AAGGACCATG ATGAGGATTT CCTTTCCCCC 
AAGAAAGTTC TAGAGTACGA GGGAGAGTTG GAGAATCATT CTCCTGCTAT CCCGAGGATC 
CCGTGTGTGT CTCCTGAGAC TGTCATGCTG AAAGAGCATG AGATGGAGCA GCACACTCCA 
CTTCCATTGC CCAAAGAGTA CGACGAGGAT TCTCTGATTC CCAGCAGTCC TGCAACTGAG 
ACTTCAGACA ATGTCAGTCC GGTTGCCAGT CCCATCCACA CCGGTTTTCT GGTGAGCTTC 
ATGGTGGATG CTCGCGGCGG CTCGATGCGC GGCAGCAGAC ACAACGGCCT CCGCGTCATC 
ATTCCTCCTC GAACCTGCGC CGCTCCCACA CGCATCACCT GCCGCCTGGT GAAGCCCCAG 
AAGCTGCCCA CACCGCCGCC ATTAGTGGAG GGAGAGGGTC TCGCCAGCCG CATCATCTCC 
CTTGGACCCG CCAGCATGCA GTTTTTAGGG CCTGTGATTG TGGAGATTCC TCATTTTGCG 
GCTCTCGGTC GGGGAGATCG GGAACTCGTG GTCTTGAGGA GTGAGAACGG ATCGGTCTGG 
AAGGAACATC GTAACCGATA TGGAGATGAA GTGCTAGAGA CCATCCTCAA CGGCATGGAT 
GAAGATCTGG AGAGTCAGGA GGAGCTCGGG AAGAAACGCA TCAGACGGAT CATCTCCACC 
GACTTCCCGC TCTACTTTGC GGTGGTGTCT CGCATCCAGC AGGAGAACGA TCTGATTGGC 
CCAGAAGGAG GTTTTCTGTC CAGTAAACTG GTGCCGATGG TCCAGGCCAC CTTTCCAGAG 
ACAGCCGTCA CCAAACGCGT GCGTCTGGGA CTGCAGGCCC AGCCGGTGCC TGATGAGCTG 
GTGGCCAAGC TTTTGGGGAA CCAGGCCACG TTCAGTCCTG TGGTGACGGT GGAGCCTCGG 
CGCCGGAAGT TTCACCGTCC CATCGGCCTA AGAATCCCAC TGCCACCCTC ATGGAGAGAG 
AGCCCGCGGG ACGCCGGAGA GGGAGACACC ACCAGCCTGC GCCTGCTCTG CAGCGTCATC 
GGTGGAACAG CTCCTGCCCA GTGGGAAGAT ATAACTGGCA CCACCAAACT GGCGTATTCC 
AATGACTGTG CCAGTTTCAC CACCAACGTC TCTGCCCGAT TCTGGCTGGC GGACTGTCCT 
CGGACTGCGG AGGCCGTGTC CTTTGCCAAC CTCCTGTACC GCGAGCTGTC GGCCGTGCCG 
TACATGGCCA AGTTTGTAGT GTTTGCGAAG ATGAATGAAG CTCGCGAGGG TCGACTCCGC 
TGCTACTGCA TGACGGATGA CAAGATGGAC AAAACCCTGG AGCAGCACGA GAACTTCTCG 
GAAGTGGCTC GCAGCAGAGA CATCGAGGTG ATGGAGGGAA TGCCGCTGTA TTTGGAGTGT 
TCAGGAAACT TGGTTCCTAT AAGAAAAGCA GCCCAGCAGC CTCGCTGTTT CAGCTTCCAG 
GCCTTTAAAG ACAACAGACT GCCTGTGTCC GTCAAGGTGA GGGACAGCAG TAAAGACCCC 
TCTGGGTTTT TGTCTTTCTT GCGCAAGTCA ACCAAATATG AAGACAGCCA ACACGTGCTG 
TGCAACCTCA ACGTGACCAT GCCGCCGTGC ATTAAGATTA TGGGCAGCGA TGAGCGGAGG 
AGGACTCTGA CTCCTCTGGC TCTCAGAGAA AGATACAGCG CTCTGAATGA ACCGGCAATG 
GCATCACTGA GCGCCATGGA AAGGACGGAG TTAAAGATGG CTTTAATAGC TGAACAGCTA 
GGCCTGAGCT GGGCTGAGTT GGGACGGGAA CTGCAGTTTA ATGTAGATGA AATAAATAAG 
ATCCGTGTGG AGAATCCCAA CTCCCTTTTA GAGCAGAGCT CAACGCTGCT CAACCTGTGG 
GCCGCCCGAG AGGGGAAGAG AGCCAAAATG GACAGCCTGT ACGTGGCCCT CAAGAGCATC 
GACCGAGTGG ACATTGTGAA CATGATGGAG GGCCAGGGAG GACCACCGGC GGGTCAGCAG 
GGGGGCTGGG AGCAGGACGC TTCTAGGTCT AGGCATCACG ACCGAGACCA TCTCTCACCT 
GGAATCACAA ATGGTTACGG GCTGGTGGTG CAGGAGGAAC TAGTGTCACC GGCCTCCATG 
CAGTACAGTC TGCCCTCGCC ACTGGGCAAT GAGCCCTACT GGCAGGAGGT TTCCAGCATG 
GAGTGCGCGC CCATCGCCAC CACTGAAGAA GACACGCTCA TGGAGATGTC CGAGGTACAG 
GTTTGGCCCT CAGGAAATAG TCCGTCCATT GTGGCTGTAG AGGACTCCTC ATTGGAGTGC 
AGCAATGCGG ACGACTCCGA GGCGCTGCAG AACCGGAGCC TGGGTCACTC CAGGAGCGGG 
ACGCCCGGTG GGAACGGACT CACTCCGGGA GGTTTAAGTG GCTCCATCGA GCTCCTGGAG 
GATCAGTCCA TCGGTGCGGA TTCAGACTAC AGTGCCGCCG GGATGCTGCG TCTGAGCGAA 
GGGGTCAACA TCAGTGCTCT GGACAGAGGT CAGAGGTCGG AGAGTTCAGA AGGGGCTGTA 
GGTCACCAAG TCACCAACAA CATTAAAGAC GGTTCCAATG GAGGTTTGGG AGAAGGAGGA 
GGTGACGGCA CACAGTTAGA AGATAGCATA TCTCTCATTT CAGGGCAACA GCGTGTGTAT 
GCTCGAATGA GCGAGTCGCC TGGGCTGAGA AGACGGGCAG ATCCTGGCGA AGACAGGTTA 
AGCGCAGGGT CGTTCCTGTC TTATCTGCAG GATCAGGGCT CTCCTGGTTG GCATTCGGCT 
GCAGAAGCGA CTCAGAACAG ATTGGCGTCT TTGCAATGCT CCCTTCCTAG GCAGGCAATA 
GAGTCTATGA TGTCATCGGT GCGAGCTACG GTGGACTCTG ACTCCAAGCA GTCTTGTTTG 
GGCCAGGAGG CTCTGATTGA GCCAGTGCGG GACATGGGGC ACTCTGAGAT CATCCATGGC 
CATTATCAGG GGACTCAGCC GTTTGAGAAA GGACTCGGCT TCCCGCACAG AACAACCAAC 
ATGCGCACTT GGGAGGACAT GCGTCTGAGC GGACAGGGTG ATGAGGCTGA GGATCTTCCA 
GGAGAGCAAG TGAGCGAGGA GCAATTCACA GACGAGCATG GCAACATCGT CACCAAAAAG 
ATTGTGAGGA AAGTGGTCCG GAGAGGAAAA GGGGAGGAAG GGGGTCAGGA GCTGATCATT 
GAAGGTCTGC AGCAGGATAT CAGTGAGCCT GACTTTGATG GAGAACAGTT CATGAGCTAT 
GCCGTCCTGG GACGTGACAG CAAGCCGGAT GTTGTGGATG TGAAGAGAGG AGGTGCTCAG 
ATAGTCAAAT GTGCGAGTCT GCGGAGAGTA AAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_021476101.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331776.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X3, mRNA.

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CloneID	ODa142593
Clone ID Related Accession (Same CDS sequence)	XM_021476101.1
Accession Version	XM_021476101.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6084bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin 1, erythrocytic a isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGAATTGC ACAGCTAACA TTAGGCGAGG AGCTGCTGGG AAACGAGGGC 
GCCAGGTATT TGATGAAGAT GGAGGACATA AAGGACCATG ATGAGGATTT CCTTTCCCCC 
AAGAAAGTTC TAGAGAATCA TTCTCCTGCT ATCCCGAGGA TCCCGTGTGT GTCTCCTGAG 
ACTGTCATGC TGAAAGAGCA TGAGATGGAG CAGCAGCACA CTCCACTTCC ATTGCCCAAA 
GAGTACGACG AGGATTCTCT GATTCCCAGC AGTCCTGCAA CTGAGACTTC AGACAATGTC 
AGTCCGGTTG CCAGTCCCAT CCACACCGGT TTTCTGGTGA GCTTCATGGT GGATGCTCGC 
GGCGGCTCGA TGCGCGGCAG CAGACACAAC GGCCTCCGCG TCATCATTCC TCCTCGAACC 
TGCGCCGCTC CCACACGCAT CACCTGCCGC CTGGTGAAGC CCCAGAAGCT GCCCACACCG 
CCGCCATTAG TGGAGGGAGA GGGTCTCGCC AGCCGCATCA TCTCCCTTGG ACCCGCCAGC 
ATGCAGTTTT TAGGGCCTGT GATTGTGGAG ATTCCTCATT TTGCGGCTCT CGGTCGGGGA 
GATCGGGAAC TCGTGGTCTT GAGGAGTGAG AACGGATCGG TCTGGAAGGA ACATCGTAAC 
CGATATGGAG ATGAAGTGCT AGAGACCATC CTCAACGGCA TGGATGAAGA TCTGGAGAGT 
CAGGAGGAGC TCGGGAAGAA ACGCATCAGA CGGATCATCT CCACCGACTT CCCGCTCTAC 
TTTGCGGTGG TGTCTCGCAT CCAGCAGGAG AACGATCTGA TTGGCCCAGA AGGAGGTTTT 
CTGTCCAGTA AACTGGTGCC GATGGTCCAG GCCACCTTTC CAGAGACAGC CGTCACCAAA 
CGCGTGCGTC TGGGACTGCA GGCCCAGCCG GTGCCTGATG AGCTGGTGGC CAAGCTTTTG 
GGGAACCAGG CCACGTTCAG TCCTGTGGTG ACGGTGGAGC CTCGGCGCCG GAAGTTTCAC 
CGTCCCATCG GCCTAAGAAT CCCACTGCCA CCCTCATGGA GAGAGAGCCC GCGGGACGCC 
GGAGAGGGAG ACACCACCAG CCTGCGCCTG CTCTGCAGCG TCATCGGTGG AACAGCTCCT 
GCCCAGTGGG AAGATATAAC TGGCACCACC AAACTGGCGT ATTCCAATGA CTGTGCCAGT 
TTCACCACCA ACGTCTCTGC CCGATTCTGG CTGGCGGACT GTCCTCGGAC TGCGGAGGCC 
GTGTCCTTTG CCAACCTCCT GTACCGCGAG CTGTCGGCCG TGCCGTACAT GGCCAAGTTT 
GTAGTGTTTG CGAAGATGAA TGAAGCTCGC GAGGGTCGAC TCCGCTGCTA CTGCATGACG 
GATGACAAGA TGGACAAAAC CCTGGAGCAG CACGAGAACT TCTCGGAAGT GGCTCGCAGC 
AGAGACATCG AGGTGATGGA GGGAATGCCG CTGTATTTGG AGTGTTCAGG AAACTTGGTT 
CCTATAAGAA AAGCAGCCCA GCAGCCTCGC TGTTTCAGCT TCCAGGCCTT TAAAGACAAC 
AGACTGCCTG TGTCCGTCAA GGTGAGGGAC AGCAGTAAAG ACCCCTCTGG GTTTTTGTCT 
TTCTTGCGCA AGTCAACCAA ATATGAAGAC AGCCAACACG TGCTGTGCAA CCTCAACGTG 
ACCATGCCGC CGTGCATTAA GATTATGGGC AGCGATGAGC GGAGGAGGAC TCTGACTCCT 
CTGGCTCTCA GAGAAAGATA CAGCGCTCTG AATGAACCGG CAATGGCATC ACTGAGCGCC 
ATGGAAAGGA CGGAGTTAAA GATGGCTTTA ATAGCTGAAC AGCTAGGCCT GAGCTGGGCT 
GAGTTGGGAC GGGAACTGCA GTTTAATGTA GATGAAATAA ATAAGATCCG TGTGGAGAAT 
CCCAACTCCC TTTTAGAGCA GAGCTCAACG CTGCTCAACC TGTGGGCCGC CCGAGAGGGG 
AAGAGAGCCA AAATGGACAG CCTGTACGTG GCCCTCAAGA GCATCGACCG AGTGGACATT 
GTGAACATGA TGGAGGGCCA GGGAGGACCA CCGGCGGGTC AGCAGGGGGG CTGGGAGCAG 
GACGCTTCTA GGTCTAGGCA TCACGACCGA GACCATCTCT CACCTGGAAT CACAAATGGT 
TACGGGCTGG TGGTGCAGGA GGAACTAGTG TCACCGGCCT CCATGCAGTA CAGTCTGCCC 
TCGCCACTGG GCAATGAGCC CTACTGGCAG GAGGTTTCCA GCATGGAGTG CGCGCCCATC 
GCCACCACTG AAGAAGACAC GCTCATGGAG ATGTCCGAGG TACAGGTTTG GCCCTCAGGA 
AATAGTCCGT CCATTGTGGC TGTAGAGGAC TCCTCATTGG AGTGCAGCAA TGCGGACGAC 
TCCGAGGCGC TGCAGAACCG GAGCCTGGGT CACTCCAGGA GCGGGACGCC CGGTGGGAAC 
GGACTCACTC CGGGAGGTTT AAGTGGCTCC ATCGAGCTCC TGGAGGATCA GTCCATCGGT 
GCGGATTCAG ACTACAGTGC CGCCGGGATG CTGCGTCTGA GCGAAGGGGT CAACATCAGT 
GCTCTGGACA GAGGTCAGAG GTCGGAGAGT TCAGAAGGGG CTGTAGGTCA CCAAGTCACC 
AACAACATTA AAGACGGTTC CAATGGAGGT TTGGGAGAAG GAGGAGGTGA CGGCACACAG 
TTAGAAGATA GCATATCTCT CATTTCAGGG CAACAGCGTG TGTATGCTCG AATGAGCGAG 
TCGCCTGGGC TGAGAAGACG GGCAGATCCT GGCGAAGACA GGTTAAGCGC AGGGTCGTTC 
CTGTCTTATC TGCAGGATCA GGGCTCTCCT GGTTGGCATT CGGCTGCAGA AGCGACTCAG 
AACAGATTGG CGTCTTTGCA ATGCTCCCTT CCTAGGCAGG CAATAGAGTC TATGATGTCA 
TCGGTGCGAG CTACGGTGGA CTCTGACTCC AAGCAGTCTT GTTTGGGCCA GGAGGCTCTG 
ATTGAGCCAG TGCGGGACAT GGGGCACTCT GAGATCATCC ATGGCCATTA TCAGGGGACT 
CAGCCGTTTG AGAAAGGACT CGGCTTCCCG CACAGAACAA CCAACATGCG CACTTGGGAG 
GACATGCGTC TGAGCGGACA GGGTGATGAG GCTGAGGATC TTCCAGGAGA GCAAGTGAGC 
GAGGAGCAAT TCACAGACGA GCATGGCAAC ATCGTCACCA AAAAGATTGT GAGGAAAGTG 
GTCCGGAGAG GAAAAGGGGA GGAAGGGGGT CAGGAGCTGA TCATTGAAGG TCTGCAGCAG 
GATATCAGTG AGCCTGACTT TGATGGAGAA CAGTTCATGA GCTATGCCGT CCTGGGACGT 
GACAGCAAGC CGGATGTTGT GGATGTGAAG AGAGGAGGTG CTCAGATAGT CAAATGTGCG 
AGTCTGCGGA GAGTAAAGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331776.1
CDS	222..6305
Translation	MAQAAKHLRKNKDLEALAEQERKEKEEEKSKKRSRSREKKRKAHVVHRWLIDQDNSVSSELPDGQGVWHYDETADAATSFLRAARSGNLEKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLINHGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGLTPLHVSSFMGHLNIVKILMQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGANPNAAGKVGLTPLHVAVHHNNLDVVNLLLSKGGSPHSAARNGYTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPLHLASQEGQPDMVLLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKHGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSNGTSALAIAKRLGYISVIDVLKLVTEETVTMTTTEKHRMSFPETVDEILDVSEDEGIAQLTLGEELLGNEGARYLMKMEDIKDHDEDFLSPKKVLENHSPAIPRIPCVSPETVMLKEHEMEQQHTPLPLPKEYDEDSLIPSSPATETSDNVSPVASPIHTGFLVSFMVDARGGSMRGSRHNGLRVIIPPRTCAAPTRITCRLVKPQKLPTPPPLVEGEGLASRIISLGPASMQFLGPVIVEIPHFAALGRGDRELVVLRSENGSVWKEHRNRYGDEVLETILNGMDEDLESQEELGKKRIRRIISTDFPLYFAVVSRIQQENDLIGPEGGFLSSKLVPMVQATFPETAVTKRVRLGLQAQPVPDELVAKLLGNQATFSPVVTVEPRRRKFHRPIGLRIPLPPSWRESPRDAGEGDTTSLRLLCSVIGGTAPAQWEDITGTTKLAYSNDCASFTTNVSARFWLADCPRTAEAVSFANLLYRELSAVPYMAKFVVFAKMNEAREGRLRCYCMTDDKMDKTLEQHENFSEVARSRDIEVMEGMPLYLECSGNLVPIRKAAQQPRCFSFQAFKDNRLPVSVKVRDSSKDPSGFLSFLRKSTKYEDSQHVLCNLNVTMPPCIKIMGSDERRRTLTPLALRERYSALNEPAMASLSAMERTELKMALIAEQLGLSWAELGRELQFNVDEINKIRVENPNSLLEQSSTLLNLWAAREGKRAKMDSLYVALKSIDRVDIVNMMEGQGGPPAGQQGGWEQDASRSRHHDRDHLSPGITNGYGLVVQEELVSPASMQYSLPSPLGNEPYWQEVSSMECAPIATTEEDTLMEMSEVQVWPSGNSPSIVAVEDSSLECSNADDSEALQNRSLGHSRSGTPGGNGLTPGGLSGSIELLEDQSIGADSDYSAAGMLRLSEGVNISALDRGQRSESSEGAVGHQVTNNIKDGSNGGLGEGGGDGTQLEDSISLISGQQRVYARMSESPGLRRRADPGEDRLSAGSFLSYLQDQGSPGWHSAAEATQNRLASLQCSLPRQAIESMMSSVRATVDSDSKQSCLGQEALIEPVRDMGHSEIIHGHYQGTQPFEKGLGFPHRTTNMRTWEDMRLSGQGDEAEDLPGEQVSEEQFTDEHGNIVTKKIVRKVVRRGKGEEGGQELIIEGLQQDISEPDFDGEQFMSYAVLGRDSKPDVVDVKRGGAQIVKCASLRRVKQ

Target ORF information:

RefSeq Version	XM_021476101.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476101.1

ORF Insert Sequence:

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGAATTGC ACAGCTAACA TTAGGCGAGG AGCTGCTGGG AAACGAGGGC 
GCCAGGTATT TGATGAAGAT GGAGGACATA AAGGACCATG ATGAGGATTT CCTTTCCCCC 
AAGAAAGTTC TAGAGAATCA TTCTCCTGCT ATCCCGAGGA TCCCGTGTGT GTCTCCTGAG 
ACTGTCATGC TGAAAGAGCA TGAGATGGAG CAGCAGCACA CTCCACTTCC ATTGCCCAAA 
GAGTACGACG AGGATTCTCT GATTCCCAGC AGTCCTGCAA CTGAGACTTC AGACAATGTC 
AGTCCGGTTG CCAGTCCCAT CCACACCGGT TTTCTGGTGA GCTTCATGGT GGATGCTCGC 
GGCGGCTCGA TGCGCGGCAG CAGACACAAC GGCCTCCGCG TCATCATTCC TCCTCGAACC 
TGCGCCGCTC CCACACGCAT CACCTGCCGC CTGGTGAAGC CCCAGAAGCT GCCCACACCG 
CCGCCATTAG TGGAGGGAGA GGGTCTCGCC AGCCGCATCA TCTCCCTTGG ACCCGCCAGC 
ATGCAGTTTT TAGGGCCTGT GATTGTGGAG ATTCCTCATT TTGCGGCTCT CGGTCGGGGA 
GATCGGGAAC TCGTGGTCTT GAGGAGTGAG AACGGATCGG TCTGGAAGGA ACATCGTAAC 
CGATATGGAG ATGAAGTGCT AGAGACCATC CTCAACGGCA TGGATGAAGA TCTGGAGAGT 
CAGGAGGAGC TCGGGAAGAA ACGCATCAGA CGGATCATCT CCACCGACTT CCCGCTCTAC 
TTTGCGGTGG TGTCTCGCAT CCAGCAGGAG AACGATCTGA TTGGCCCAGA AGGAGGTTTT 
CTGTCCAGTA AACTGGTGCC GATGGTCCAG GCCACCTTTC CAGAGACAGC CGTCACCAAA 
CGCGTGCGTC TGGGACTGCA GGCCCAGCCG GTGCCTGATG AGCTGGTGGC CAAGCTTTTG 
GGGAACCAGG CCACGTTCAG TCCTGTGGTG ACGGTGGAGC CTCGGCGCCG GAAGTTTCAC 
CGTCCCATCG GCCTAAGAAT CCCACTGCCA CCCTCATGGA GAGAGAGCCC GCGGGACGCC 
GGAGAGGGAG ACACCACCAG CCTGCGCCTG CTCTGCAGCG TCATCGGTGG AACAGCTCCT 
GCCCAGTGGG AAGATATAAC TGGCACCACC AAACTGGCGT ATTCCAATGA CTGTGCCAGT 
TTCACCACCA ACGTCTCTGC CCGATTCTGG CTGGCGGACT GTCCTCGGAC TGCGGAGGCC 
GTGTCCTTTG CCAACCTCCT GTACCGCGAG CTGTCGGCCG TGCCGTACAT GGCCAAGTTT 
GTAGTGTTTG CGAAGATGAA TGAAGCTCGC GAGGGTCGAC TCCGCTGCTA CTGCATGACG 
GATGACAAGA TGGACAAAAC CCTGGAGCAG CACGAGAACT TCTCGGAAGT GGCTCGCAGC 
AGAGACATCG AGGTGATGGA GGGAATGCCG CTGTATTTGG AGTGTTCAGG AAACTTGGTT 
CCTATAAGAA AAGCAGCCCA GCAGCCTCGC TGTTTCAGCT TCCAGGCCTT TAAAGACAAC 
AGACTGCCTG TGTCCGTCAA GGTGAGGGAC AGCAGTAAAG ACCCCTCTGG GTTTTTGTCT 
TTCTTGCGCA AGTCAACCAA ATATGAAGAC AGCCAACACG TGCTGTGCAA CCTCAACGTG 
ACCATGCCGC CGTGCATTAA GATTATGGGC AGCGATGAGC GGAGGAGGAC TCTGACTCCT 
CTGGCTCTCA GAGAAAGATA CAGCGCTCTG AATGAACCGG CAATGGCATC ACTGAGCGCC 
ATGGAAAGGA CGGAGTTAAA GATGGCTTTA ATAGCTGAAC AGCTAGGCCT GAGCTGGGCT 
GAGTTGGGAC GGGAACTGCA GTTTAATGTA GATGAAATAA ATAAGATCCG TGTGGAGAAT 
CCCAACTCCC TTTTAGAGCA GAGCTCAACG CTGCTCAACC TGTGGGCCGC CCGAGAGGGG 
AAGAGAGCCA AAATGGACAG CCTGTACGTG GCCCTCAAGA GCATCGACCG AGTGGACATT 
GTGAACATGA TGGAGGGCCA GGGAGGACCA CCGGCGGGTC AGCAGGGGGG CTGGGAGCAG 
GACGCTTCTA GGTCTAGGCA TCACGACCGA GACCATCTCT CACCTGGAAT CACAAATGGT 
TACGGGCTGG TGGTGCAGGA GGAACTAGTG TCACCGGCCT CCATGCAGTA CAGTCTGCCC 
TCGCCACTGG GCAATGAGCC CTACTGGCAG GAGGTTTCCA GCATGGAGTG CGCGCCCATC 
GCCACCACTG AAGAAGACAC GCTCATGGAG ATGTCCGAGG TACAGGTTTG GCCCTCAGGA 
AATAGTCCGT CCATTGTGGC TGTAGAGGAC TCCTCATTGG AGTGCAGCAA TGCGGACGAC 
TCCGAGGCGC TGCAGAACCG GAGCCTGGGT CACTCCAGGA GCGGGACGCC CGGTGGGAAC 
GGACTCACTC CGGGAGGTTT AAGTGGCTCC ATCGAGCTCC TGGAGGATCA GTCCATCGGT 
GCGGATTCAG ACTACAGTGC CGCCGGGATG CTGCGTCTGA GCGAAGGGGT CAACATCAGT 
GCTCTGGACA GAGGTCAGAG GTCGGAGAGT TCAGAAGGGG CTGTAGGTCA CCAAGTCACC 
AACAACATTA AAGACGGTTC CAATGGAGGT TTGGGAGAAG GAGGAGGTGA CGGCACACAG 
TTAGAAGATA GCATATCTCT CATTTCAGGG CAACAGCGTG TGTATGCTCG AATGAGCGAG 
TCGCCTGGGC TGAGAAGACG GGCAGATCCT GGCGAAGACA GGTTAAGCGC AGGGTCGTTC 
CTGTCTTATC TGCAGGATCA GGGCTCTCCT GGTTGGCATT CGGCTGCAGA AGCGACTCAG 
AACAGATTGG CGTCTTTGCA ATGCTCCCTT CCTAGGCAGG CAATAGAGTC TATGATGTCA 
TCGGTGCGAG CTACGGTGGA CTCTGACTCC AAGCAGTCTT GTTTGGGCCA GGAGGCTCTG 
ATTGAGCCAG TGCGGGACAT GGGGCACTCT GAGATCATCC ATGGCCATTA TCAGGGGACT 
CAGCCGTTTG AGAAAGGACT CGGCTTCCCG CACAGAACAA CCAACATGCG CACTTGGGAG 
GACATGCGTC TGAGCGGACA GGGTGATGAG GCTGAGGATC TTCCAGGAGA GCAAGTGAGC 
GAGGAGCAAT TCACAGACGA GCATGGCAAC ATCGTCACCA AAAAGATTGT GAGGAAAGTG 
GTCCGGAGAG GAAAAGGGGA GGAAGGGGGT CAGGAGCTGA TCATTGAAGG TCTGCAGCAG 
GATATCAGTG AGCCTGACTT TGATGGAGAA CAGTTCATGA GCTATGCCGT CCTGGGACGT 
GACAGCAAGC CGGATGTTGT GGATGTGAAG AGAGGAGGTG CTCAGATAGT CAAATGTGCG 
AGTCTGCGGA GAGTAAAGCA GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_021476102.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331777.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X4, mRNA.

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CloneID	ODa142594
Clone ID Related Accession (Same CDS sequence)	XM_021476102.1
Accession Version	XM_021476102.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6081bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin 1, erythrocytic a isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGCGAGGA GCTGCTGGGA AACGAGGGCG CCAGGTATTT GATGAAGATG 
GAGGACATAA AGGACCATGA TGAGGATTTC CTTTCCCCCA AGAAAGTTCT AGAGTACGAG 
GGAGAGTTGG AGAATCATTC TCCTGCTATC CCGAGGATCC CGTGTGTGTC TCCTGAGACT 
GTCATGCTGA AAGAGCATGA GATGGAGCAG CAGCACACTC CACTTCCATT GCCCAAAGAG 
TACGACGAGG ATTCTCTGAT TCCCAGCAGT CCTGCAACTG AGACTTCAGA CAATGTCAGT 
CCGGTTGCCA GTCCCATCCA CACCGGTTTT CTGGTGAGCT TCATGGTGGA TGCTCGCGGC 
GGCTCGATGC GCGGCAGCAG ACACAACGGC CTCCGCGTCA TCATTCCTCC TCGAACCTGC 
GCCGCTCCCA CACGCATCAC CTGCCGCCTG GTGAAGCCCC AGAAGCTGCC CACACCGCCG 
CCATTAGTGG AGGGAGAGGG TCTCGCCAGC CGCATCATCT CCCTTGGACC CGCCAGCATG 
CAGTTTTTAG GGCCTGTGAT TGTGGAGATT CCTCATTTTG CGGCTCTCGG TCGGGGAGAT 
CGGGAACTCG TGGTCTTGAG GAGTGAGAAC GGATCGGTCT GGAAGGAACA TCGTAACCGA 
TATGGAGATG AAGTGCTAGA GACCATCCTC AACGGCATGG ATGAAGATCT GGAGAGTCAG 
GAGGAGCTCG GGAAGAAACG CATCAGACGG ATCATCTCCA CCGACTTCCC GCTCTACTTT 
GCGGTGGTGT CTCGCATCCA GCAGGAGAAC GATCTGATTG GCCCAGAAGG AGGTTTTCTG 
TCCAGTAAAC TGGTGCCGAT GGTCCAGGCC ACCTTTCCAG AGACAGCCGT CACCAAACGC 
GTGCGTCTGG GACTGCAGGC CCAGCCGGTG CCTGATGAGC TGGTGGCCAA GCTTTTGGGG 
AACCAGGCCA CGTTCAGTCC TGTGGTGACG GTGGAGCCTC GGCGCCGGAA GTTTCACCGT 
CCCATCGGCC TAAGAATCCC ACTGCCACCC TCATGGAGAG AGAGCCCGCG GGACGCCGGA 
GAGGGAGACA CCACCAGCCT GCGCCTGCTC TGCAGCGTCA TCGGTGGAAC AGCTCCTGCC 
CAGTGGGAAG ATATAACTGG CACCACCAAA CTGGCGTATT CCAATGACTG TGCCAGTTTC 
ACCACCAACG TCTCTGCCCG ATTCTGGCTG GCGGACTGTC CTCGGACTGC GGAGGCCGTG 
TCCTTTGCCA ACCTCCTGTA CCGCGAGCTG TCGGCCGTGC CGTACATGGC CAAGTTTGTA 
GTGTTTGCGA AGATGAATGA AGCTCGCGAG GGTCGACTCC GCTGCTACTG CATGACGGAT 
GACAAGATGG ACAAAACCCT GGAGCAGCAC GAGAACTTCT CGGAAGTGGC TCGCAGCAGA 
GACATCGAGG TGATGGAGGG AATGCCGCTG TATTTGGAGT GTTCAGGAAA CTTGGTTCCT 
ATAAGAAAAG CAGCCCAGCA GCCTCGCTGT TTCAGCTTCC AGGCCTTTAA AGACAACAGA 
CTGCCTGTGT CCGTCAAGGT GAGGGACAGC AGTAAAGACC CCTCTGGGTT TTTGTCTTTC 
TTGCGCAAGT CAACCAAATA TGAAGACAGC CAACACGTGC TGTGCAACCT CAACGTGACC 
ATGCCGCCGT GCATTAAGAT TATGGGCAGC GATGAGCGGA GGAGGACTCT GACTCCTCTG 
GCTCTCAGAG AAAGATACAG CGCTCTGAAT GAACCGGCAA TGGCATCACT GAGCGCCATG 
GAAAGGACGG AGTTAAAGAT GGCTTTAATA GCTGAACAGC TAGGCCTGAG CTGGGCTGAG 
TTGGGACGGG AACTGCAGTT TAATGTAGAT GAAATAAATA AGATCCGTGT GGAGAATCCC 
AACTCCCTTT TAGAGCAGAG CTCAACGCTG CTCAACCTGT GGGCCGCCCG AGAGGGGAAG 
AGAGCCAAAA TGGACAGCCT GTACGTGGCC CTCAAGAGCA TCGACCGAGT GGACATTGTG 
AACATGATGG AGGGCCAGGG AGGACCACCG GCGGGTCAGC AGGGGGGCTG GGAGCAGGAC 
GCTTCTAGGT CTAGGCATCA CGACCGAGAC CATCTCTCAC CTGGAATCAC AAATGGTTAC 
GGGCTGGTGG TGCAGGAGGA ACTAGTGTCA CCGGCCTCCA TGCAGTACAG TCTGCCCTCG 
CCACTGGGCA ATGAGCCCTA CTGGCAGGAG GTTTCCAGCA TGGAGTGCGC GCCCATCGCC 
ACCACTGAAG AAGACACGCT CATGGAGATG TCCGAGGTAC AGGTTTGGCC CTCAGGAAAT 
AGTCCGTCCA TTGTGGCTGT AGAGGACTCC TCATTGGAGT GCAGCAATGC GGACGACTCC 
GAGGCGCTGC AGAACCGGAG CCTGGGTCAC TCCAGGAGCG GGACGCCCGG TGGGAACGGA 
CTCACTCCGG GAGGTTTAAG TGGCTCCATC GAGCTCCTGG AGGATCAGTC CATCGGTGCG 
GATTCAGACT ACAGTGCCGC CGGGATGCTG CGTCTGAGCG AAGGGGTCAA CATCAGTGCT 
CTGGACAGAG GTCAGAGGTC GGAGAGTTCA GAAGGGGCTG TAGGTCACCA AGTCACCAAC 
AACATTAAAG ACGGTTCCAA TGGAGGTTTG GGAGAAGGAG GAGGTGACGG CACACAGTTA 
GAAGATAGCA TATCTCTCAT TTCAGGGCAA CAGCGTGTGT ATGCTCGAAT GAGCGAGTCG 
CCTGGGCTGA GAAGACGGGC AGATCCTGGC GAAGACAGGT TAAGCGCAGG GTCGTTCCTG 
TCTTATCTGC AGGATCAGGG CTCTCCTGGT TGGCATTCGG CTGCAGAAGC GACTCAGAAC 
AGATTGGCGT CTTTGCAATG CTCCCTTCCT AGGCAGGCAA TAGAGTCTAT GATGTCATCG 
GTGCGAGCTA CGGTGGACTC TGACTCCAAG CAGTCTTGTT TGGGCCAGGA GGCTCTGATT 
GAGCCAGTGC GGGACATGGG GCACTCTGAG ATCATCCATG GCCATTATCA GGGGACTCAG 
CCGTTTGAGA AAGGACTCGG CTTCCCGCAC AGAACAACCA ACATGCGCAC TTGGGAGGAC 
ATGCGTCTGA GCGGACAGGG TGATGAGGCT GAGGATCTTC CAGGAGAGCA AGTGAGCGAG 
GAGCAATTCA CAGACGAGCA TGGCAACATC GTCACCAAAA AGATTGTGAG GAAAGTGGTC 
CGGAGAGGAA AAGGGGAGGA AGGGGGTCAG GAGCTGATCA TTGAAGGTCT GCAGCAGGAT 
ATCAGTGAGC CTGACTTTGA TGGAGAACAG TTCATGAGCT ATGCCGTCCT GGGACGTGAC 
AGCAAGCCGG ATGTTGTGGA TGTGAAGAGA GGAGGTGCTC AGATAGTCAA ATGTGCGAGT 
CTGCGGAGAG TAAAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331777.1
CDS	222..6302
Translation	MAQAAKHLRKNKDLEALAEQERKEKEEEKSKKRSRSREKKRKAHVVHRWLIDQDNSVSSELPDGQGVWHYDETADAATSFLRAARSGNLEKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLINHGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGLTPLHVSSFMGHLNIVKILMQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGANPNAAGKVGLTPLHVAVHHNNLDVVNLLLSKGGSPHSAARNGYTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPLHLASQEGQPDMVLLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKHGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSNGTSALAIAKRLGYISVIDVLKLVTEETVTMTTTEKHRMSFPETVDEILDVSEDEGEELLGNEGARYLMKMEDIKDHDEDFLSPKKVLEYEGELENHSPAIPRIPCVSPETVMLKEHEMEQQHTPLPLPKEYDEDSLIPSSPATETSDNVSPVASPIHTGFLVSFMVDARGGSMRGSRHNGLRVIIPPRTCAAPTRITCRLVKPQKLPTPPPLVEGEGLASRIISLGPASMQFLGPVIVEIPHFAALGRGDRELVVLRSENGSVWKEHRNRYGDEVLETILNGMDEDLESQEELGKKRIRRIISTDFPLYFAVVSRIQQENDLIGPEGGFLSSKLVPMVQATFPETAVTKRVRLGLQAQPVPDELVAKLLGNQATFSPVVTVEPRRRKFHRPIGLRIPLPPSWRESPRDAGEGDTTSLRLLCSVIGGTAPAQWEDITGTTKLAYSNDCASFTTNVSARFWLADCPRTAEAVSFANLLYRELSAVPYMAKFVVFAKMNEAREGRLRCYCMTDDKMDKTLEQHENFSEVARSRDIEVMEGMPLYLECSGNLVPIRKAAQQPRCFSFQAFKDNRLPVSVKVRDSSKDPSGFLSFLRKSTKYEDSQHVLCNLNVTMPPCIKIMGSDERRRTLTPLALRERYSALNEPAMASLSAMERTELKMALIAEQLGLSWAELGRELQFNVDEINKIRVENPNSLLEQSSTLLNLWAAREGKRAKMDSLYVALKSIDRVDIVNMMEGQGGPPAGQQGGWEQDASRSRHHDRDHLSPGITNGYGLVVQEELVSPASMQYSLPSPLGNEPYWQEVSSMECAPIATTEEDTLMEMSEVQVWPSGNSPSIVAVEDSSLECSNADDSEALQNRSLGHSRSGTPGGNGLTPGGLSGSIELLEDQSIGADSDYSAAGMLRLSEGVNISALDRGQRSESSEGAVGHQVTNNIKDGSNGGLGEGGGDGTQLEDSISLISGQQRVYARMSESPGLRRRADPGEDRLSAGSFLSYLQDQGSPGWHSAAEATQNRLASLQCSLPRQAIESMMSSVRATVDSDSKQSCLGQEALIEPVRDMGHSEIIHGHYQGTQPFEKGLGFPHRTTNMRTWEDMRLSGQGDEAEDLPGEQVSEEQFTDEHGNIVTKKIVRKVVRRGKGEEGGQELIIEGLQQDISEPDFDGEQFMSYAVLGRDSKPDVVDVKRGGAQIVKCASLRRVKQ

Target ORF information:

RefSeq Version	XM_021476102.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476102.1

ORF Insert Sequence:

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGCGAGGA GCTGCTGGGA AACGAGGGCG CCAGGTATTT GATGAAGATG 
GAGGACATAA AGGACCATGA TGAGGATTTC CTTTCCCCCA AGAAAGTTCT AGAGTACGAG 
GGAGAGTTGG AGAATCATTC TCCTGCTATC CCGAGGATCC CGTGTGTGTC TCCTGAGACT 
GTCATGCTGA AAGAGCATGA GATGGAGCAG CAGCACACTC CACTTCCATT GCCCAAAGAG 
TACGACGAGG ATTCTCTGAT TCCCAGCAGT CCTGCAACTG AGACTTCAGA CAATGTCAGT 
CCGGTTGCCA GTCCCATCCA CACCGGTTTT CTGGTGAGCT TCATGGTGGA TGCTCGCGGC 
GGCTCGATGC GCGGCAGCAG ACACAACGGC CTCCGCGTCA TCATTCCTCC TCGAACCTGC 
GCCGCTCCCA CACGCATCAC CTGCCGCCTG GTGAAGCCCC AGAAGCTGCC CACACCGCCG 
CCATTAGTGG AGGGAGAGGG TCTCGCCAGC CGCATCATCT CCCTTGGACC CGCCAGCATG 
CAGTTTTTAG GGCCTGTGAT TGTGGAGATT CCTCATTTTG CGGCTCTCGG TCGGGGAGAT 
CGGGAACTCG TGGTCTTGAG GAGTGAGAAC GGATCGGTCT GGAAGGAACA TCGTAACCGA 
TATGGAGATG AAGTGCTAGA GACCATCCTC AACGGCATGG ATGAAGATCT GGAGAGTCAG 
GAGGAGCTCG GGAAGAAACG CATCAGACGG ATCATCTCCA CCGACTTCCC GCTCTACTTT 
GCGGTGGTGT CTCGCATCCA GCAGGAGAAC GATCTGATTG GCCCAGAAGG AGGTTTTCTG 
TCCAGTAAAC TGGTGCCGAT GGTCCAGGCC ACCTTTCCAG AGACAGCCGT CACCAAACGC 
GTGCGTCTGG GACTGCAGGC CCAGCCGGTG CCTGATGAGC TGGTGGCCAA GCTTTTGGGG 
AACCAGGCCA CGTTCAGTCC TGTGGTGACG GTGGAGCCTC GGCGCCGGAA GTTTCACCGT 
CCCATCGGCC TAAGAATCCC ACTGCCACCC TCATGGAGAG AGAGCCCGCG GGACGCCGGA 
GAGGGAGACA CCACCAGCCT GCGCCTGCTC TGCAGCGTCA TCGGTGGAAC AGCTCCTGCC 
CAGTGGGAAG ATATAACTGG CACCACCAAA CTGGCGTATT CCAATGACTG TGCCAGTTTC 
ACCACCAACG TCTCTGCCCG ATTCTGGCTG GCGGACTGTC CTCGGACTGC GGAGGCCGTG 
TCCTTTGCCA ACCTCCTGTA CCGCGAGCTG TCGGCCGTGC CGTACATGGC CAAGTTTGTA 
GTGTTTGCGA AGATGAATGA AGCTCGCGAG GGTCGACTCC GCTGCTACTG CATGACGGAT 
GACAAGATGG ACAAAACCCT GGAGCAGCAC GAGAACTTCT CGGAAGTGGC TCGCAGCAGA 
GACATCGAGG TGATGGAGGG AATGCCGCTG TATTTGGAGT GTTCAGGAAA CTTGGTTCCT 
ATAAGAAAAG CAGCCCAGCA GCCTCGCTGT TTCAGCTTCC AGGCCTTTAA AGACAACAGA 
CTGCCTGTGT CCGTCAAGGT GAGGGACAGC AGTAAAGACC CCTCTGGGTT TTTGTCTTTC 
TTGCGCAAGT CAACCAAATA TGAAGACAGC CAACACGTGC TGTGCAACCT CAACGTGACC 
ATGCCGCCGT GCATTAAGAT TATGGGCAGC GATGAGCGGA GGAGGACTCT GACTCCTCTG 
GCTCTCAGAG AAAGATACAG CGCTCTGAAT GAACCGGCAA TGGCATCACT GAGCGCCATG 
GAAAGGACGG AGTTAAAGAT GGCTTTAATA GCTGAACAGC TAGGCCTGAG CTGGGCTGAG 
TTGGGACGGG AACTGCAGTT TAATGTAGAT GAAATAAATA AGATCCGTGT GGAGAATCCC 
AACTCCCTTT TAGAGCAGAG CTCAACGCTG CTCAACCTGT GGGCCGCCCG AGAGGGGAAG 
AGAGCCAAAA TGGACAGCCT GTACGTGGCC CTCAAGAGCA TCGACCGAGT GGACATTGTG 
AACATGATGG AGGGCCAGGG AGGACCACCG GCGGGTCAGC AGGGGGGCTG GGAGCAGGAC 
GCTTCTAGGT CTAGGCATCA CGACCGAGAC CATCTCTCAC CTGGAATCAC AAATGGTTAC 
GGGCTGGTGG TGCAGGAGGA ACTAGTGTCA CCGGCCTCCA TGCAGTACAG TCTGCCCTCG 
CCACTGGGCA ATGAGCCCTA CTGGCAGGAG GTTTCCAGCA TGGAGTGCGC GCCCATCGCC 
ACCACTGAAG AAGACACGCT CATGGAGATG TCCGAGGTAC AGGTTTGGCC CTCAGGAAAT 
AGTCCGTCCA TTGTGGCTGT AGAGGACTCC TCATTGGAGT GCAGCAATGC GGACGACTCC 
GAGGCGCTGC AGAACCGGAG CCTGGGTCAC TCCAGGAGCG GGACGCCCGG TGGGAACGGA 
CTCACTCCGG GAGGTTTAAG TGGCTCCATC GAGCTCCTGG AGGATCAGTC CATCGGTGCG 
GATTCAGACT ACAGTGCCGC CGGGATGCTG CGTCTGAGCG AAGGGGTCAA CATCAGTGCT 
CTGGACAGAG GTCAGAGGTC GGAGAGTTCA GAAGGGGCTG TAGGTCACCA AGTCACCAAC 
AACATTAAAG ACGGTTCCAA TGGAGGTTTG GGAGAAGGAG GAGGTGACGG CACACAGTTA 
GAAGATAGCA TATCTCTCAT TTCAGGGCAA CAGCGTGTGT ATGCTCGAAT GAGCGAGTCG 
CCTGGGCTGA GAAGACGGGC AGATCCTGGC GAAGACAGGT TAAGCGCAGG GTCGTTCCTG 
TCTTATCTGC AGGATCAGGG CTCTCCTGGT TGGCATTCGG CTGCAGAAGC GACTCAGAAC 
AGATTGGCGT CTTTGCAATG CTCCCTTCCT AGGCAGGCAA TAGAGTCTAT GATGTCATCG 
GTGCGAGCTA CGGTGGACTC TGACTCCAAG CAGTCTTGTT TGGGCCAGGA GGCTCTGATT 
GAGCCAGTGC GGGACATGGG GCACTCTGAG ATCATCCATG GCCATTATCA GGGGACTCAG 
CCGTTTGAGA AAGGACTCGG CTTCCCGCAC AGAACAACCA ACATGCGCAC TTGGGAGGAC 
ATGCGTCTGA GCGGACAGGG TGATGAGGCT GAGGATCTTC CAGGAGAGCA AGTGAGCGAG 
GAGCAATTCA CAGACGAGCA TGGCAACATC GTCACCAAAA AGATTGTGAG GAAAGTGGTC 
CGGAGAGGAA AAGGGGAGGA AGGGGGTCAG GAGCTGATCA TTGAAGGTCT GCAGCAGGAT 
ATCAGTGAGC CTGACTTTGA TGGAGAACAG TTCATGAGCT ATGCCGTCCT GGGACGTGAC 
AGCAAGCCGG ATGTTGTGGA TGTGAAGAGA GGAGGTGCTC AGATAGTCAA ATGTGCGAGT 
CTGCGGAGAG TAAAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_021476103.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331778.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X5, mRNA.

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CloneID	ODa142595
Clone ID Related Accession (Same CDS sequence)	XM_021476103.1
Accession Version	XM_021476103.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6081bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin 1, erythrocytic a isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGAATTGC ACAGCTAACA TTAGGCGAGG AGCTGCTGGG AAACGAGGGC 
GCCAGGTATT TGATGAAGAT GGAGGACATA AAGGACCATG ATGAGGATTT CCTTTCCCCC 
AAGAAAGTTC TAGAGAATCA TTCTCCTGCT ATCCCGAGGA TCCCGTGTGT GTCTCCTGAG 
ACTGTCATGC TGAAAGAGCA TGAGATGGAG CAGCACACTC CACTTCCATT GCCCAAAGAG 
TACGACGAGG ATTCTCTGAT TCCCAGCAGT CCTGCAACTG AGACTTCAGA CAATGTCAGT 
CCGGTTGCCA GTCCCATCCA CACCGGTTTT CTGGTGAGCT TCATGGTGGA TGCTCGCGGC 
GGCTCGATGC GCGGCAGCAG ACACAACGGC CTCCGCGTCA TCATTCCTCC TCGAACCTGC 
GCCGCTCCCA CACGCATCAC CTGCCGCCTG GTGAAGCCCC AGAAGCTGCC CACACCGCCG 
CCATTAGTGG AGGGAGAGGG TCTCGCCAGC CGCATCATCT CCCTTGGACC CGCCAGCATG 
CAGTTTTTAG GGCCTGTGAT TGTGGAGATT CCTCATTTTG CGGCTCTCGG TCGGGGAGAT 
CGGGAACTCG TGGTCTTGAG GAGTGAGAAC GGATCGGTCT GGAAGGAACA TCGTAACCGA 
TATGGAGATG AAGTGCTAGA GACCATCCTC AACGGCATGG ATGAAGATCT GGAGAGTCAG 
GAGGAGCTCG GGAAGAAACG CATCAGACGG ATCATCTCCA CCGACTTCCC GCTCTACTTT 
GCGGTGGTGT CTCGCATCCA GCAGGAGAAC GATCTGATTG GCCCAGAAGG AGGTTTTCTG 
TCCAGTAAAC TGGTGCCGAT GGTCCAGGCC ACCTTTCCAG AGACAGCCGT CACCAAACGC 
GTGCGTCTGG GACTGCAGGC CCAGCCGGTG CCTGATGAGC TGGTGGCCAA GCTTTTGGGG 
AACCAGGCCA CGTTCAGTCC TGTGGTGACG GTGGAGCCTC GGCGCCGGAA GTTTCACCGT 
CCCATCGGCC TAAGAATCCC ACTGCCACCC TCATGGAGAG AGAGCCCGCG GGACGCCGGA 
GAGGGAGACA CCACCAGCCT GCGCCTGCTC TGCAGCGTCA TCGGTGGAAC AGCTCCTGCC 
CAGTGGGAAG ATATAACTGG CACCACCAAA CTGGCGTATT CCAATGACTG TGCCAGTTTC 
ACCACCAACG TCTCTGCCCG ATTCTGGCTG GCGGACTGTC CTCGGACTGC GGAGGCCGTG 
TCCTTTGCCA ACCTCCTGTA CCGCGAGCTG TCGGCCGTGC CGTACATGGC CAAGTTTGTA 
GTGTTTGCGA AGATGAATGA AGCTCGCGAG GGTCGACTCC GCTGCTACTG CATGACGGAT 
GACAAGATGG ACAAAACCCT GGAGCAGCAC GAGAACTTCT CGGAAGTGGC TCGCAGCAGA 
GACATCGAGG TGATGGAGGG AATGCCGCTG TATTTGGAGT GTTCAGGAAA CTTGGTTCCT 
ATAAGAAAAG CAGCCCAGCA GCCTCGCTGT TTCAGCTTCC AGGCCTTTAA AGACAACAGA 
CTGCCTGTGT CCGTCAAGGT GAGGGACAGC AGTAAAGACC CCTCTGGGTT TTTGTCTTTC 
TTGCGCAAGT CAACCAAATA TGAAGACAGC CAACACGTGC TGTGCAACCT CAACGTGACC 
ATGCCGCCGT GCATTAAGAT TATGGGCAGC GATGAGCGGA GGAGGACTCT GACTCCTCTG 
GCTCTCAGAG AAAGATACAG CGCTCTGAAT GAACCGGCAA TGGCATCACT GAGCGCCATG 
GAAAGGACGG AGTTAAAGAT GGCTTTAATA GCTGAACAGC TAGGCCTGAG CTGGGCTGAG 
TTGGGACGGG AACTGCAGTT TAATGTAGAT GAAATAAATA AGATCCGTGT GGAGAATCCC 
AACTCCCTTT TAGAGCAGAG CTCAACGCTG CTCAACCTGT GGGCCGCCCG AGAGGGGAAG 
AGAGCCAAAA TGGACAGCCT GTACGTGGCC CTCAAGAGCA TCGACCGAGT GGACATTGTG 
AACATGATGG AGGGCCAGGG AGGACCACCG GCGGGTCAGC AGGGGGGCTG GGAGCAGGAC 
GCTTCTAGGT CTAGGCATCA CGACCGAGAC CATCTCTCAC CTGGAATCAC AAATGGTTAC 
GGGCTGGTGG TGCAGGAGGA ACTAGTGTCA CCGGCCTCCA TGCAGTACAG TCTGCCCTCG 
CCACTGGGCA ATGAGCCCTA CTGGCAGGAG GTTTCCAGCA TGGAGTGCGC GCCCATCGCC 
ACCACTGAAG AAGACACGCT CATGGAGATG TCCGAGGTAC AGGTTTGGCC CTCAGGAAAT 
AGTCCGTCCA TTGTGGCTGT AGAGGACTCC TCATTGGAGT GCAGCAATGC GGACGACTCC 
GAGGCGCTGC AGAACCGGAG CCTGGGTCAC TCCAGGAGCG GGACGCCCGG TGGGAACGGA 
CTCACTCCGG GAGGTTTAAG TGGCTCCATC GAGCTCCTGG AGGATCAGTC CATCGGTGCG 
GATTCAGACT ACAGTGCCGC CGGGATGCTG CGTCTGAGCG AAGGGGTCAA CATCAGTGCT 
CTGGACAGAG GTCAGAGGTC GGAGAGTTCA GAAGGGGCTG TAGGTCACCA AGTCACCAAC 
AACATTAAAG ACGGTTCCAA TGGAGGTTTG GGAGAAGGAG GAGGTGACGG CACACAGTTA 
GAAGATAGCA TATCTCTCAT TTCAGGGCAA CAGCGTGTGT ATGCTCGAAT GAGCGAGTCG 
CCTGGGCTGA GAAGACGGGC AGATCCTGGC GAAGACAGGT TAAGCGCAGG GTCGTTCCTG 
TCTTATCTGC AGGATCAGGG CTCTCCTGGT TGGCATTCGG CTGCAGAAGC GACTCAGAAC 
AGATTGGCGT CTTTGCAATG CTCCCTTCCT AGGCAGGCAA TAGAGTCTAT GATGTCATCG 
GTGCGAGCTA CGGTGGACTC TGACTCCAAG CAGTCTTGTT TGGGCCAGGA GGCTCTGATT 
GAGCCAGTGC GGGACATGGG GCACTCTGAG ATCATCCATG GCCATTATCA GGGGACTCAG 
CCGTTTGAGA AAGGACTCGG CTTCCCGCAC AGAACAACCA ACATGCGCAC TTGGGAGGAC 
ATGCGTCTGA GCGGACAGGG TGATGAGGCT GAGGATCTTC CAGGAGAGCA AGTGAGCGAG 
GAGCAATTCA CAGACGAGCA TGGCAACATC GTCACCAAAA AGATTGTGAG GAAAGTGGTC 
CGGAGAGGAA AAGGGGAGGA AGGGGGTCAG GAGCTGATCA TTGAAGGTCT GCAGCAGGAT 
ATCAGTGAGC CTGACTTTGA TGGAGAACAG TTCATGAGCT ATGCCGTCCT GGGACGTGAC 
AGCAAGCCGG ATGTTGTGGA TGTGAAGAGA GGAGGTGCTC AGATAGTCAA ATGTGCGAGT 
CTGCGGAGAG TAAAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331778.1
CDS	222..6302
Translation	MAQAAKHLRKNKDLEALAEQERKEKEEEKSKKRSRSREKKRKAHVVHRWLIDQDNSVSSELPDGQGVWHYDETADAATSFLRAARSGNLEKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLINHGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGLTPLHVSSFMGHLNIVKILMQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGANPNAAGKVGLTPLHVAVHHNNLDVVNLLLSKGGSPHSAARNGYTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPLHLASQEGQPDMVLLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKHGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSNGTSALAIAKRLGYISVIDVLKLVTEETVTMTTTEKHRMSFPETVDEILDVSEDEGIAQLTLGEELLGNEGARYLMKMEDIKDHDEDFLSPKKVLENHSPAIPRIPCVSPETVMLKEHEMEQHTPLPLPKEYDEDSLIPSSPATETSDNVSPVASPIHTGFLVSFMVDARGGSMRGSRHNGLRVIIPPRTCAAPTRITCRLVKPQKLPTPPPLVEGEGLASRIISLGPASMQFLGPVIVEIPHFAALGRGDRELVVLRSENGSVWKEHRNRYGDEVLETILNGMDEDLESQEELGKKRIRRIISTDFPLYFAVVSRIQQENDLIGPEGGFLSSKLVPMVQATFPETAVTKRVRLGLQAQPVPDELVAKLLGNQATFSPVVTVEPRRRKFHRPIGLRIPLPPSWRESPRDAGEGDTTSLRLLCSVIGGTAPAQWEDITGTTKLAYSNDCASFTTNVSARFWLADCPRTAEAVSFANLLYRELSAVPYMAKFVVFAKMNEAREGRLRCYCMTDDKMDKTLEQHENFSEVARSRDIEVMEGMPLYLECSGNLVPIRKAAQQPRCFSFQAFKDNRLPVSVKVRDSSKDPSGFLSFLRKSTKYEDSQHVLCNLNVTMPPCIKIMGSDERRRTLTPLALRERYSALNEPAMASLSAMERTELKMALIAEQLGLSWAELGRELQFNVDEINKIRVENPNSLLEQSSTLLNLWAAREGKRAKMDSLYVALKSIDRVDIVNMMEGQGGPPAGQQGGWEQDASRSRHHDRDHLSPGITNGYGLVVQEELVSPASMQYSLPSPLGNEPYWQEVSSMECAPIATTEEDTLMEMSEVQVWPSGNSPSIVAVEDSSLECSNADDSEALQNRSLGHSRSGTPGGNGLTPGGLSGSIELLEDQSIGADSDYSAAGMLRLSEGVNISALDRGQRSESSEGAVGHQVTNNIKDGSNGGLGEGGGDGTQLEDSISLISGQQRVYARMSESPGLRRRADPGEDRLSAGSFLSYLQDQGSPGWHSAAEATQNRLASLQCSLPRQAIESMMSSVRATVDSDSKQSCLGQEALIEPVRDMGHSEIIHGHYQGTQPFEKGLGFPHRTTNMRTWEDMRLSGQGDEAEDLPGEQVSEEQFTDEHGNIVTKKIVRKVVRRGKGEEGGQELIIEGLQQDISEPDFDGEQFMSYAVLGRDSKPDVVDVKRGGAQIVKCASLRRVKQ

Target ORF information:

RefSeq Version	XM_021476103.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476103.1

ORF Insert Sequence:

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGAATTGC ACAGCTAACA TTAGGCGAGG AGCTGCTGGG AAACGAGGGC 
GCCAGGTATT TGATGAAGAT GGAGGACATA AAGGACCATG ATGAGGATTT CCTTTCCCCC 
AAGAAAGTTC TAGAGAATCA TTCTCCTGCT ATCCCGAGGA TCCCGTGTGT GTCTCCTGAG 
ACTGTCATGC TGAAAGAGCA TGAGATGGAG CAGCACACTC CACTTCCATT GCCCAAAGAG 
TACGACGAGG ATTCTCTGAT TCCCAGCAGT CCTGCAACTG AGACTTCAGA CAATGTCAGT 
CCGGTTGCCA GTCCCATCCA CACCGGTTTT CTGGTGAGCT TCATGGTGGA TGCTCGCGGC 
GGCTCGATGC GCGGCAGCAG ACACAACGGC CTCCGCGTCA TCATTCCTCC TCGAACCTGC 
GCCGCTCCCA CACGCATCAC CTGCCGCCTG GTGAAGCCCC AGAAGCTGCC CACACCGCCG 
CCATTAGTGG AGGGAGAGGG TCTCGCCAGC CGCATCATCT CCCTTGGACC CGCCAGCATG 
CAGTTTTTAG GGCCTGTGAT TGTGGAGATT CCTCATTTTG CGGCTCTCGG TCGGGGAGAT 
CGGGAACTCG TGGTCTTGAG GAGTGAGAAC GGATCGGTCT GGAAGGAACA TCGTAACCGA 
TATGGAGATG AAGTGCTAGA GACCATCCTC AACGGCATGG ATGAAGATCT GGAGAGTCAG 
GAGGAGCTCG GGAAGAAACG CATCAGACGG ATCATCTCCA CCGACTTCCC GCTCTACTTT 
GCGGTGGTGT CTCGCATCCA GCAGGAGAAC GATCTGATTG GCCCAGAAGG AGGTTTTCTG 
TCCAGTAAAC TGGTGCCGAT GGTCCAGGCC ACCTTTCCAG AGACAGCCGT CACCAAACGC 
GTGCGTCTGG GACTGCAGGC CCAGCCGGTG CCTGATGAGC TGGTGGCCAA GCTTTTGGGG 
AACCAGGCCA CGTTCAGTCC TGTGGTGACG GTGGAGCCTC GGCGCCGGAA GTTTCACCGT 
CCCATCGGCC TAAGAATCCC ACTGCCACCC TCATGGAGAG AGAGCCCGCG GGACGCCGGA 
GAGGGAGACA CCACCAGCCT GCGCCTGCTC TGCAGCGTCA TCGGTGGAAC AGCTCCTGCC 
CAGTGGGAAG ATATAACTGG CACCACCAAA CTGGCGTATT CCAATGACTG TGCCAGTTTC 
ACCACCAACG TCTCTGCCCG ATTCTGGCTG GCGGACTGTC CTCGGACTGC GGAGGCCGTG 
TCCTTTGCCA ACCTCCTGTA CCGCGAGCTG TCGGCCGTGC CGTACATGGC CAAGTTTGTA 
GTGTTTGCGA AGATGAATGA AGCTCGCGAG GGTCGACTCC GCTGCTACTG CATGACGGAT 
GACAAGATGG ACAAAACCCT GGAGCAGCAC GAGAACTTCT CGGAAGTGGC TCGCAGCAGA 
GACATCGAGG TGATGGAGGG AATGCCGCTG TATTTGGAGT GTTCAGGAAA CTTGGTTCCT 
ATAAGAAAAG CAGCCCAGCA GCCTCGCTGT TTCAGCTTCC AGGCCTTTAA AGACAACAGA 
CTGCCTGTGT CCGTCAAGGT GAGGGACAGC AGTAAAGACC CCTCTGGGTT TTTGTCTTTC 
TTGCGCAAGT CAACCAAATA TGAAGACAGC CAACACGTGC TGTGCAACCT CAACGTGACC 
ATGCCGCCGT GCATTAAGAT TATGGGCAGC GATGAGCGGA GGAGGACTCT GACTCCTCTG 
GCTCTCAGAG AAAGATACAG CGCTCTGAAT GAACCGGCAA TGGCATCACT GAGCGCCATG 
GAAAGGACGG AGTTAAAGAT GGCTTTAATA GCTGAACAGC TAGGCCTGAG CTGGGCTGAG 
TTGGGACGGG AACTGCAGTT TAATGTAGAT GAAATAAATA AGATCCGTGT GGAGAATCCC 
AACTCCCTTT TAGAGCAGAG CTCAACGCTG CTCAACCTGT GGGCCGCCCG AGAGGGGAAG 
AGAGCCAAAA TGGACAGCCT GTACGTGGCC CTCAAGAGCA TCGACCGAGT GGACATTGTG 
AACATGATGG AGGGCCAGGG AGGACCACCG GCGGGTCAGC AGGGGGGCTG GGAGCAGGAC 
GCTTCTAGGT CTAGGCATCA CGACCGAGAC CATCTCTCAC CTGGAATCAC AAATGGTTAC 
GGGCTGGTGG TGCAGGAGGA ACTAGTGTCA CCGGCCTCCA TGCAGTACAG TCTGCCCTCG 
CCACTGGGCA ATGAGCCCTA CTGGCAGGAG GTTTCCAGCA TGGAGTGCGC GCCCATCGCC 
ACCACTGAAG AAGACACGCT CATGGAGATG TCCGAGGTAC AGGTTTGGCC CTCAGGAAAT 
AGTCCGTCCA TTGTGGCTGT AGAGGACTCC TCATTGGAGT GCAGCAATGC GGACGACTCC 
GAGGCGCTGC AGAACCGGAG CCTGGGTCAC TCCAGGAGCG GGACGCCCGG TGGGAACGGA 
CTCACTCCGG GAGGTTTAAG TGGCTCCATC GAGCTCCTGG AGGATCAGTC CATCGGTGCG 
GATTCAGACT ACAGTGCCGC CGGGATGCTG CGTCTGAGCG AAGGGGTCAA CATCAGTGCT 
CTGGACAGAG GTCAGAGGTC GGAGAGTTCA GAAGGGGCTG TAGGTCACCA AGTCACCAAC 
AACATTAAAG ACGGTTCCAA TGGAGGTTTG GGAGAAGGAG GAGGTGACGG CACACAGTTA 
GAAGATAGCA TATCTCTCAT TTCAGGGCAA CAGCGTGTGT ATGCTCGAAT GAGCGAGTCG 
CCTGGGCTGA GAAGACGGGC AGATCCTGGC GAAGACAGGT TAAGCGCAGG GTCGTTCCTG 
TCTTATCTGC AGGATCAGGG CTCTCCTGGT TGGCATTCGG CTGCAGAAGC GACTCAGAAC 
AGATTGGCGT CTTTGCAATG CTCCCTTCCT AGGCAGGCAA TAGAGTCTAT GATGTCATCG 
GTGCGAGCTA CGGTGGACTC TGACTCCAAG CAGTCTTGTT TGGGCCAGGA GGCTCTGATT 
GAGCCAGTGC GGGACATGGG GCACTCTGAG ATCATCCATG GCCATTATCA GGGGACTCAG 
CCGTTTGAGA AAGGACTCGG CTTCCCGCAC AGAACAACCA ACATGCGCAC TTGGGAGGAC 
ATGCGTCTGA GCGGACAGGG TGATGAGGCT GAGGATCTTC CAGGAGAGCA AGTGAGCGAG 
GAGCAATTCA CAGACGAGCA TGGCAACATC GTCACCAAAA AGATTGTGAG GAAAGTGGTC 
CGGAGAGGAA AAGGGGAGGA AGGGGGTCAG GAGCTGATCA TTGAAGGTCT GCAGCAGGAT 
ATCAGTGAGC CTGACTTTGA TGGAGAACAG TTCATGAGCT ATGCCGTCCT GGGACGTGAC 
AGCAAGCCGG ATGTTGTGGA TGTGAAGAGA GGAGGTGCTC AGATAGTCAA ATGTGCGAGT 
CTGCGGAGAG TAAAGCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_021476104.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331779.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X6, mRNA.

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CloneID	ODa142596
Clone ID Related Accession (Same CDS sequence)	XM_021476104.1
Accession Version	XM_021476104.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6069bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin 1, erythrocytic a isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGAATTGC ACAGCTAACA TTAGGCGAGG AGCTGCTGGG AAACGAGGGC 
GCCAGGTATT TGATGAAGAT GGAGGACATA AAGGACCATG ATGAGGATTT CCTTTCCCCC 
AAGAAAGTTC TAGAGTACGA GGGAGAGTTG GAGAATCATT CTCCTGCTAT CCCGAGGATC 
CCGTGTGTGT CTCCTGAGAC TGTCATGCTG AAAGAGCATG AGATGGAGCA GCAGCACACT 
CCACTTCCAT TGCCCAAAGA GTACGACGAG GATTCTCTGA TTCCCAGCAG TCCTGCAACT 
GAGACTTCAG ACAATGTCAG TCCGGTTGCC AGTCCCATCC ACACCGGTTT TCTGGTGAGC 
TTCATGGTGG ATGCTCGCGG CGGCTCGATG CGCGGCAGCA GACACAACGG CCTCCGCGTC 
ATCATTCCTC CTCGAACCTG CGCCGCTCCC ACACGCATCA CCTGCCGCCT GGTGAAGCCC 
CAGAAGCTGC CCACACCGCC GCCATTAGTG GAGGGAGAGG GTCTCGCCAG CCGCATCATC 
TCCCTTGGAC CCGCCAGCAT GCAGTTTTTA GGGCCTGTGA TTGTGGAGAT TCCTCATTTT 
GCGGCTCTCG GTCGGGGAGA TCGGGAACTC GTGGTCTTGA GGAGTGAGAA CGGATCGGTC 
TGGAAGGAAC ATCGTAACCG ATATGGAGAT GAAGTGCTAG AGACCATCCT CAACGGCATG 
GATGAAGATC TGGAGAGTCA GGAGGAGCTC GGGAAGAAAC GCATCAGACG GATCATCTCC 
ACCGACTTCC CGCTCTACTT TGCGGTGGTG TCTCGCATCC AGCAGGAGAA CGATCTGATT 
GGCCCAGAAG GAGGTTTTCT GTCCAGTAAA CTGGTGCCGA TGGTCCAGGC CACCTTTCCA 
GAGACAGCCG TCACCAAACG CGTGCGTCTG GGACTGCAGG CCCAGCCGGT GCCTGATGAG 
CTGGTGGCCA AGCTTTTGGG GAACCAGGCC ACGTTCAGTC CTGTGGTGAC GGTGGAGCCT 
CGGCGCCGGA AGTTTCACCG TCCCATCGGC CTAAGAATCC CACTGCCACC CTCATGGAGA 
GAGAGCCCGC GGGACGCCGG AGAGGGAGAC ACCACCAGCC TGCGCCTGCT CTGCAGCGTC 
ATCGGTGGAA CAGCTCCTGC CCAGTGGGAA GATATAACTG GCACCACCAA ACTGGCGTAT 
TCCAATGACT GTGCCAGTTT CACCACCAAC GTCTCTGCCC GATTCTGGCT GGCGGACTGT 
CCTCGGACTG CGGAGGCCGT GTCCTTTGCC AACCTCCTGT ACCGCGAGCT GTCGGCCGTG 
CCGTACATGG CCAAGTTTGT AGTGTTTGCG AAGATGAATG AAGCTCGCGA GGGTCGACTC 
CGCTGCTACT GCATGACGGA TGACAAGATG GACAAAACCC TGGAGCAGCA CGAGAACTTC 
TCGGAAGTGG CTCGCAGCAG AGACATCGAG GTGATGGAGG GAATGCCGCT GTATTTGGAG 
TGTTCAGGAA ACTTGGTTCC TATAAGAAAA GCAGCCCAGC AGCCTCGCTG TTTCAGCTTC 
CAGGCCTTTA AAGACAACAG ACTGCCTGTG TCCGTCAAGG TGAGGGACAG CAGTAAAGAC 
CCCTCTGGGT TTTTGTCTTT CTTGCGCAAG TCAACCAAAT ATGAAGACAG CCAACACGTG 
CTGTGCAACC TCAACGTGAC CATGCCGCCG TGCATTAAGA TTATGGGCAG CGATGAGCGG 
AGGAGGACTC TGACTCCTCT GGCTCTCAGA GAAAGATACA GCGCTCTGAA TGAACCGGCA 
ATGGCATCAC TGAGCGCCAT GGAAAGGACG GAGTTAAAGA TGGCTTTAAT AGCTGAACAG 
CTAGGCCTGA GCTGGGCTGA GTTGGGACGG GAACTGCAGT TTAATGTAGA TGAAATAAAT 
AAGATCCGTG TGGAGAATCC CAACTCCCTT TTAGAGCAGA GCTCAACGCT GCTCAACCTG 
TGGGCCGCCC GAGAGGGGAA GAGAGCCAAA ATGGACAGCC TGTACGTGGC CCTCAAGAGC 
ATCGACCGAG TGGACATTGT GAACATGATG GAGGGCCAGG GAGGACCACC GGCGGGTCAG 
CAGGGGGGCT GGGAGCAGGA CGCTTCTAGG TCTAGGCATC ACGACCGAGA CCATCTCTCA 
CCTGGAATCA CAAATGGTTA CGGGCTGGTG GTGCAGGAGG AACTAGTGTC ACCGGCCTCC 
ATGCAGTACA GTCTGCCCTC GCCACTGGGC AATGAGCCCT ACTGGCAGGA GGTTTCCAGC 
ATGGAGTGCG CGCCCATCGC CACCACTGAA GAAGACACGC TCATGGAGAT GTCCGAGGTA 
CAGGTTTGGC CCTCAGGAAA TAGTCCGTCC ATTGTGGCTG TAGAGGACTC CTCATTGGAG 
TGCAGCAATG CGGACGACTC CGAGGCGCTG CAGAACCGGA GCCTGGGTCA CTCCAGGAGC 
GGGACGCCCG GTGGGAACGG ACTCACTCCG GGAGGTTTAA GTGGCTCCAT CGAGCTCCTG 
GAGGATCAGT CCATCGGTGC GGATTCAGAC TACAGTGCCG CCGGGATGCT GCGTCTGAGC 
GAAGGGGTCA ACATCAGTGC TCTGGACAGA GGTCAGAGGT CGGAGAGTTC AGAAGGGGCT 
GTAGGTCACC AAGTCACCAA CAACATTAAA GACGGTTCCA ATGGAGGTTT GGGAGAAGGA 
GGAGGTGACG GCACACAGTT AGAAGATAGC ATATCTCTCA TTTCAGGGCA ACAGCGTGTG 
TATGCTCGAA TGAGCGAGTC GCCTGGGCTG AGAAGACGGG CAGATCCTGG CGAAGACAGG 
TTAAGCGCAG GGTCGTTCCT GTCTTATCTG CAGGATCAGG GCTCTCCTGG TTGGCATTCG 
GCTGCAGAAG CGACTCAGAA CAGATTGGCG TCTTTGCAAT GCTCCCTTCC TAGGCAGGCA 
ATAGAGTCTA TGATGTCATC GGTGCGAGCT ACGGTGGACT CTGACTCCAA GCAGTCTTGT 
TTGGGCCAGG AGGCTCTGAT TGAGCCAGTG CGGGACATGG GGCACTCTGA GATCATCCAT 
GGCCATTATC AGGGGACTCA GCCGTTTGAG AAAGGACTCG GCTTCCCGCA CAGAACAACC 
AACATGCGCA CTTGGGAGGA CATGCGTCTG AGCGGACAGG GTGATGAGGC TGAGGATCTT 
CCAGGAGAGC AAGTGAGCGA GGAGCAATTC ACAGACGAGC ATGGCAACAT CGTCACCAAA 
AAGATTGTGA GGAAAGTGGT CCGGAGAGGA AAAGGGGAGG AAGGGGGTCA GGAGCTGATC 
ATTGAAGGTC TGCAGCAGGA TATCAGTGAG CCTGACTTTG ATGGAGAACA GTTCATGAGC 
TATGCCGTCC TGGGACGTGA CAGCAAGGAC TCGACTCCTT CACCCAAACC ATCCTTCACT 
GACTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331779.1
CDS	222..6290
Translation	MAQAAKHLRKNKDLEALAEQERKEKEEEKSKKRSRSREKKRKAHVVHRWLIDQDNSVSSELPDGQGVWHYDETADAATSFLRAARSGNLEKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLINHGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGLTPLHVSSFMGHLNIVKILMQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGANPNAAGKVGLTPLHVAVHHNNLDVVNLLLSKGGSPHSAARNGYTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPLHLASQEGQPDMVLLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKHGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSNGTSALAIAKRLGYISVIDVLKLVTEETVTMTTTEKHRMSFPETVDEILDVSEDEGIAQLTLGEELLGNEGARYLMKMEDIKDHDEDFLSPKKVLEYEGELENHSPAIPRIPCVSPETVMLKEHEMEQQHTPLPLPKEYDEDSLIPSSPATETSDNVSPVASPIHTGFLVSFMVDARGGSMRGSRHNGLRVIIPPRTCAAPTRITCRLVKPQKLPTPPPLVEGEGLASRIISLGPASMQFLGPVIVEIPHFAALGRGDRELVVLRSENGSVWKEHRNRYGDEVLETILNGMDEDLESQEELGKKRIRRIISTDFPLYFAVVSRIQQENDLIGPEGGFLSSKLVPMVQATFPETAVTKRVRLGLQAQPVPDELVAKLLGNQATFSPVVTVEPRRRKFHRPIGLRIPLPPSWRESPRDAGEGDTTSLRLLCSVIGGTAPAQWEDITGTTKLAYSNDCASFTTNVSARFWLADCPRTAEAVSFANLLYRELSAVPYMAKFVVFAKMNEAREGRLRCYCMTDDKMDKTLEQHENFSEVARSRDIEVMEGMPLYLECSGNLVPIRKAAQQPRCFSFQAFKDNRLPVSVKVRDSSKDPSGFLSFLRKSTKYEDSQHVLCNLNVTMPPCIKIMGSDERRRTLTPLALRERYSALNEPAMASLSAMERTELKMALIAEQLGLSWAELGRELQFNVDEINKIRVENPNSLLEQSSTLLNLWAAREGKRAKMDSLYVALKSIDRVDIVNMMEGQGGPPAGQQGGWEQDASRSRHHDRDHLSPGITNGYGLVVQEELVSPASMQYSLPSPLGNEPYWQEVSSMECAPIATTEEDTLMEMSEVQVWPSGNSPSIVAVEDSSLECSNADDSEALQNRSLGHSRSGTPGGNGLTPGGLSGSIELLEDQSIGADSDYSAAGMLRLSEGVNISALDRGQRSESSEGAVGHQVTNNIKDGSNGGLGEGGGDGTQLEDSISLISGQQRVYARMSESPGLRRRADPGEDRLSAGSFLSYLQDQGSPGWHSAAEATQNRLASLQCSLPRQAIESMMSSVRATVDSDSKQSCLGQEALIEPVRDMGHSEIIHGHYQGTQPFEKGLGFPHRTTNMRTWEDMRLSGQGDEAEDLPGEQVSEEQFTDEHGNIVTKKIVRKVVRRGKGEEGGQELIIEGLQQDISEPDFDGEQFMSYAVLGRDSKDSTPSPKPSFTDS

Target ORF information:

RefSeq Version	XM_021476104.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476104.1

ORF Insert Sequence:

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGAATTGC ACAGCTAACA TTAGGCGAGG AGCTGCTGGG AAACGAGGGC 
GCCAGGTATT TGATGAAGAT GGAGGACATA AAGGACCATG ATGAGGATTT CCTTTCCCCC 
AAGAAAGTTC TAGAGTACGA GGGAGAGTTG GAGAATCATT CTCCTGCTAT CCCGAGGATC 
CCGTGTGTGT CTCCTGAGAC TGTCATGCTG AAAGAGCATG AGATGGAGCA GCAGCACACT 
CCACTTCCAT TGCCCAAAGA GTACGACGAG GATTCTCTGA TTCCCAGCAG TCCTGCAACT 
GAGACTTCAG ACAATGTCAG TCCGGTTGCC AGTCCCATCC ACACCGGTTT TCTGGTGAGC 
TTCATGGTGG ATGCTCGCGG CGGCTCGATG CGCGGCAGCA GACACAACGG CCTCCGCGTC 
ATCATTCCTC CTCGAACCTG CGCCGCTCCC ACACGCATCA CCTGCCGCCT GGTGAAGCCC 
CAGAAGCTGC CCACACCGCC GCCATTAGTG GAGGGAGAGG GTCTCGCCAG CCGCATCATC 
TCCCTTGGAC CCGCCAGCAT GCAGTTTTTA GGGCCTGTGA TTGTGGAGAT TCCTCATTTT 
GCGGCTCTCG GTCGGGGAGA TCGGGAACTC GTGGTCTTGA GGAGTGAGAA CGGATCGGTC 
TGGAAGGAAC ATCGTAACCG ATATGGAGAT GAAGTGCTAG AGACCATCCT CAACGGCATG 
GATGAAGATC TGGAGAGTCA GGAGGAGCTC GGGAAGAAAC GCATCAGACG GATCATCTCC 
ACCGACTTCC CGCTCTACTT TGCGGTGGTG TCTCGCATCC AGCAGGAGAA CGATCTGATT 
GGCCCAGAAG GAGGTTTTCT GTCCAGTAAA CTGGTGCCGA TGGTCCAGGC CACCTTTCCA 
GAGACAGCCG TCACCAAACG CGTGCGTCTG GGACTGCAGG CCCAGCCGGT GCCTGATGAG 
CTGGTGGCCA AGCTTTTGGG GAACCAGGCC ACGTTCAGTC CTGTGGTGAC GGTGGAGCCT 
CGGCGCCGGA AGTTTCACCG TCCCATCGGC CTAAGAATCC CACTGCCACC CTCATGGAGA 
GAGAGCCCGC GGGACGCCGG AGAGGGAGAC ACCACCAGCC TGCGCCTGCT CTGCAGCGTC 
ATCGGTGGAA CAGCTCCTGC CCAGTGGGAA GATATAACTG GCACCACCAA ACTGGCGTAT 
TCCAATGACT GTGCCAGTTT CACCACCAAC GTCTCTGCCC GATTCTGGCT GGCGGACTGT 
CCTCGGACTG CGGAGGCCGT GTCCTTTGCC AACCTCCTGT ACCGCGAGCT GTCGGCCGTG 
CCGTACATGG CCAAGTTTGT AGTGTTTGCG AAGATGAATG AAGCTCGCGA GGGTCGACTC 
CGCTGCTACT GCATGACGGA TGACAAGATG GACAAAACCC TGGAGCAGCA CGAGAACTTC 
TCGGAAGTGG CTCGCAGCAG AGACATCGAG GTGATGGAGG GAATGCCGCT GTATTTGGAG 
TGTTCAGGAA ACTTGGTTCC TATAAGAAAA GCAGCCCAGC AGCCTCGCTG TTTCAGCTTC 
CAGGCCTTTA AAGACAACAG ACTGCCTGTG TCCGTCAAGG TGAGGGACAG CAGTAAAGAC 
CCCTCTGGGT TTTTGTCTTT CTTGCGCAAG TCAACCAAAT ATGAAGACAG CCAACACGTG 
CTGTGCAACC TCAACGTGAC CATGCCGCCG TGCATTAAGA TTATGGGCAG CGATGAGCGG 
AGGAGGACTC TGACTCCTCT GGCTCTCAGA GAAAGATACA GCGCTCTGAA TGAACCGGCA 
ATGGCATCAC TGAGCGCCAT GGAAAGGACG GAGTTAAAGA TGGCTTTAAT AGCTGAACAG 
CTAGGCCTGA GCTGGGCTGA GTTGGGACGG GAACTGCAGT TTAATGTAGA TGAAATAAAT 
AAGATCCGTG TGGAGAATCC CAACTCCCTT TTAGAGCAGA GCTCAACGCT GCTCAACCTG 
TGGGCCGCCC GAGAGGGGAA GAGAGCCAAA ATGGACAGCC TGTACGTGGC CCTCAAGAGC 
ATCGACCGAG TGGACATTGT GAACATGATG GAGGGCCAGG GAGGACCACC GGCGGGTCAG 
CAGGGGGGCT GGGAGCAGGA CGCTTCTAGG TCTAGGCATC ACGACCGAGA CCATCTCTCA 
CCTGGAATCA CAAATGGTTA CGGGCTGGTG GTGCAGGAGG AACTAGTGTC ACCGGCCTCC 
ATGCAGTACA GTCTGCCCTC GCCACTGGGC AATGAGCCCT ACTGGCAGGA GGTTTCCAGC 
ATGGAGTGCG CGCCCATCGC CACCACTGAA GAAGACACGC TCATGGAGAT GTCCGAGGTA 
CAGGTTTGGC CCTCAGGAAA TAGTCCGTCC ATTGTGGCTG TAGAGGACTC CTCATTGGAG 
TGCAGCAATG CGGACGACTC CGAGGCGCTG CAGAACCGGA GCCTGGGTCA CTCCAGGAGC 
GGGACGCCCG GTGGGAACGG ACTCACTCCG GGAGGTTTAA GTGGCTCCAT CGAGCTCCTG 
GAGGATCAGT CCATCGGTGC GGATTCAGAC TACAGTGCCG CCGGGATGCT GCGTCTGAGC 
GAAGGGGTCA ACATCAGTGC TCTGGACAGA GGTCAGAGGT CGGAGAGTTC AGAAGGGGCT 
GTAGGTCACC AAGTCACCAA CAACATTAAA GACGGTTCCA ATGGAGGTTT GGGAGAAGGA 
GGAGGTGACG GCACACAGTT AGAAGATAGC ATATCTCTCA TTTCAGGGCA ACAGCGTGTG 
TATGCTCGAA TGAGCGAGTC GCCTGGGCTG AGAAGACGGG CAGATCCTGG CGAAGACAGG 
TTAAGCGCAG GGTCGTTCCT GTCTTATCTG CAGGATCAGG GCTCTCCTGG TTGGCATTCG 
GCTGCAGAAG CGACTCAGAA CAGATTGGCG TCTTTGCAAT GCTCCCTTCC TAGGCAGGCA 
ATAGAGTCTA TGATGTCATC GGTGCGAGCT ACGGTGGACT CTGACTCCAA GCAGTCTTGT 
TTGGGCCAGG AGGCTCTGAT TGAGCCAGTG CGGGACATGG GGCACTCTGA GATCATCCAT 
GGCCATTATC AGGGGACTCA GCCGTTTGAG AAAGGACTCG GCTTCCCGCA CAGAACAACC 
AACATGCGCA CTTGGGAGGA CATGCGTCTG AGCGGACAGG GTGATGAGGC TGAGGATCTT 
CCAGGAGAGC AAGTGAGCGA GGAGCAATTC ACAGACGAGC ATGGCAACAT CGTCACCAAA 
AAGATTGTGA GGAAAGTGGT CCGGAGAGGA AAAGGGGAGG AAGGGGGTCA GGAGCTGATC 
ATTGAAGGTC TGCAGCAGGA TATCAGTGAG CCTGACTTTG ATGGAGAACA GTTCATGAGC 
TATGCCGTCC TGGGACGTGA CAGCAAGGAC TCGACTCCTT CACCCAAACC ATCCTTCACT 
GACTCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_021476105.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331780.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X7, mRNA.

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CloneID	ODa142597
Clone ID Related Accession (Same CDS sequence)	XM_021476105.1
Accession Version	XM_021476105.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6063bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin 1, erythrocytic a isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGCGAGGA GCTGCTGGGA AACGAGGGCG CCAGGTATTT GATGAAGATG 
GAGGACATAA AGGACCATGA TGAGGATTTC CTTTCCCCCA AGAAAGTTCT AGAGAATCAT 
TCTCCTGCTA TCCCGAGGAT CCCGTGTGTG TCTCCTGAGA CTGTCATGCT GAAAGAGCAT 
GAGATGGAGC AGCAGCACAC TCCACTTCCA TTGCCCAAAG AGTACGACGA GGATTCTCTG 
ATTCCCAGCA GTCCTGCAAC TGAGACTTCA GACAATGTCA GTCCGGTTGC CAGTCCCATC 
CACACCGGTT TTCTGGTGAG CTTCATGGTG GATGCTCGCG GCGGCTCGAT GCGCGGCAGC 
AGACACAACG GCCTCCGCGT CATCATTCCT CCTCGAACCT GCGCCGCTCC CACACGCATC 
ACCTGCCGCC TGGTGAAGCC CCAGAAGCTG CCCACACCGC CGCCATTAGT GGAGGGAGAG 
GGTCTCGCCA GCCGCATCAT CTCCCTTGGA CCCGCCAGCA TGCAGTTTTT AGGGCCTGTG 
ATTGTGGAGA TTCCTCATTT TGCGGCTCTC GGTCGGGGAG ATCGGGAACT CGTGGTCTTG 
AGGAGTGAGA ACGGATCGGT CTGGAAGGAA CATCGTAACC GATATGGAGA TGAAGTGCTA 
GAGACCATCC TCAACGGCAT GGATGAAGAT CTGGAGAGTC AGGAGGAGCT CGGGAAGAAA 
CGCATCAGAC GGATCATCTC CACCGACTTC CCGCTCTACT TTGCGGTGGT GTCTCGCATC 
CAGCAGGAGA ACGATCTGAT TGGCCCAGAA GGAGGTTTTC TGTCCAGTAA ACTGGTGCCG 
ATGGTCCAGG CCACCTTTCC AGAGACAGCC GTCACCAAAC GCGTGCGTCT GGGACTGCAG 
GCCCAGCCGG TGCCTGATGA GCTGGTGGCC AAGCTTTTGG GGAACCAGGC CACGTTCAGT 
CCTGTGGTGA CGGTGGAGCC TCGGCGCCGG AAGTTTCACC GTCCCATCGG CCTAAGAATC 
CCACTGCCAC CCTCATGGAG AGAGAGCCCG CGGGACGCCG GAGAGGGAGA CACCACCAGC 
CTGCGCCTGC TCTGCAGCGT CATCGGTGGA ACAGCTCCTG CCCAGTGGGA AGATATAACT 
GGCACCACCA AACTGGCGTA TTCCAATGAC TGTGCCAGTT TCACCACCAA CGTCTCTGCC 
CGATTCTGGC TGGCGGACTG TCCTCGGACT GCGGAGGCCG TGTCCTTTGC CAACCTCCTG 
TACCGCGAGC TGTCGGCCGT GCCGTACATG GCCAAGTTTG TAGTGTTTGC GAAGATGAAT 
GAAGCTCGCG AGGGTCGACT CCGCTGCTAC TGCATGACGG ATGACAAGAT GGACAAAACC 
CTGGAGCAGC ACGAGAACTT CTCGGAAGTG GCTCGCAGCA GAGACATCGA GGTGATGGAG 
GGAATGCCGC TGTATTTGGA GTGTTCAGGA AACTTGGTTC CTATAAGAAA AGCAGCCCAG 
CAGCCTCGCT GTTTCAGCTT CCAGGCCTTT AAAGACAACA GACTGCCTGT GTCCGTCAAG 
GTGAGGGACA GCAGTAAAGA CCCCTCTGGG TTTTTGTCTT TCTTGCGCAA GTCAACCAAA 
TATGAAGACA GCCAACACGT GCTGTGCAAC CTCAACGTGA CCATGCCGCC GTGCATTAAG 
ATTATGGGCA GCGATGAGCG GAGGAGGACT CTGACTCCTC TGGCTCTCAG AGAAAGATAC 
AGCGCTCTGA ATGAACCGGC AATGGCATCA CTGAGCGCCA TGGAAAGGAC GGAGTTAAAG 
ATGGCTTTAA TAGCTGAACA GCTAGGCCTG AGCTGGGCTG AGTTGGGACG GGAACTGCAG 
TTTAATGTAG ATGAAATAAA TAAGATCCGT GTGGAGAATC CCAACTCCCT TTTAGAGCAG 
AGCTCAACGC TGCTCAACCT GTGGGCCGCC CGAGAGGGGA AGAGAGCCAA AATGGACAGC 
CTGTACGTGG CCCTCAAGAG CATCGACCGA GTGGACATTG TGAACATGAT GGAGGGCCAG 
GGAGGACCAC CGGCGGGTCA GCAGGGGGGC TGGGAGCAGG ACGCTTCTAG GTCTAGGCAT 
CACGACCGAG ACCATCTCTC ACCTGGAATC ACAAATGGTT ACGGGCTGGT GGTGCAGGAG 
GAACTAGTGT CACCGGCCTC CATGCAGTAC AGTCTGCCCT CGCCACTGGG CAATGAGCCC 
TACTGGCAGG AGGTTTCCAG CATGGAGTGC GCGCCCATCG CCACCACTGA AGAAGACACG 
CTCATGGAGA TGTCCGAGGT ACAGGTTTGG CCCTCAGGAA ATAGTCCGTC CATTGTGGCT 
GTAGAGGACT CCTCATTGGA GTGCAGCAAT GCGGACGACT CCGAGGCGCT GCAGAACCGG 
AGCCTGGGTC ACTCCAGGAG CGGGACGCCC GGTGGGAACG GACTCACTCC GGGAGGTTTA 
AGTGGCTCCA TCGAGCTCCT GGAGGATCAG TCCATCGGTG CGGATTCAGA CTACAGTGCC 
GCCGGGATGC TGCGTCTGAG CGAAGGGGTC AACATCAGTG CTCTGGACAG AGGTCAGAGG 
TCGGAGAGTT CAGAAGGGGC TGTAGGTCAC CAAGTCACCA ACAACATTAA AGACGGTTCC 
AATGGAGGTT TGGGAGAAGG AGGAGGTGAC GGCACACAGT TAGAAGATAG CATATCTCTC 
ATTTCAGGGC AACAGCGTGT GTATGCTCGA ATGAGCGAGT CGCCTGGGCT GAGAAGACGG 
GCAGATCCTG GCGAAGACAG GTTAAGCGCA GGGTCGTTCC TGTCTTATCT GCAGGATCAG 
GGCTCTCCTG GTTGGCATTC GGCTGCAGAA GCGACTCAGA ACAGATTGGC GTCTTTGCAA 
TGCTCCCTTC CTAGGCAGGC AATAGAGTCT ATGATGTCAT CGGTGCGAGC TACGGTGGAC 
TCTGACTCCA AGCAGTCTTG TTTGGGCCAG GAGGCTCTGA TTGAGCCAGT GCGGGACATG 
GGGCACTCTG AGATCATCCA TGGCCATTAT CAGGGGACTC AGCCGTTTGA GAAAGGACTC 
GGCTTCCCGC ACAGAACAAC CAACATGCGC ACTTGGGAGG ACATGCGTCT GAGCGGACAG 
GGTGATGAGG CTGAGGATCT TCCAGGAGAG CAAGTGAGCG AGGAGCAATT CACAGACGAG 
CATGGCAACA TCGTCACCAA AAAGATTGTG AGGAAAGTGG TCCGGAGAGG AAAAGGGGAG 
GAAGGGGGTC AGGAGCTGAT CATTGAAGGT CTGCAGCAGG ATATCAGTGA GCCTGACTTT 
GATGGAGAAC AGTTCATGAG CTATGCCGTC CTGGGACGTG ACAGCAAGCC GGATGTTGTG 
GATGTGAAGA GAGGAGGTGC TCAGATAGTC AAATGTGCGA GTCTGCGGAG AGTAAAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331780.1
CDS	221..6283
Translation	MAQAAKHLRKNKDLEALAEQERKEKEEEKSKKRSRSREKKRKAHVVHRWLIDQDNSVSSELPDGQGVWHYDETADAATSFLRAARSGNLEKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLINHGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGLTPLHVSSFMGHLNIVKILMQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGANPNAAGKVGLTPLHVAVHHNNLDVVNLLLSKGGSPHSAARNGYTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPLHLASQEGQPDMVLLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKHGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSNGTSALAIAKRLGYISVIDVLKLVTEETVTMTTTEKHRMSFPETVDEILDVSEDEGEELLGNEGARYLMKMEDIKDHDEDFLSPKKVLENHSPAIPRIPCVSPETVMLKEHEMEQQHTPLPLPKEYDEDSLIPSSPATETSDNVSPVASPIHTGFLVSFMVDARGGSMRGSRHNGLRVIIPPRTCAAPTRITCRLVKPQKLPTPPPLVEGEGLASRIISLGPASMQFLGPVIVEIPHFAALGRGDRELVVLRSENGSVWKEHRNRYGDEVLETILNGMDEDLESQEELGKKRIRRIISTDFPLYFAVVSRIQQENDLIGPEGGFLSSKLVPMVQATFPETAVTKRVRLGLQAQPVPDELVAKLLGNQATFSPVVTVEPRRRKFHRPIGLRIPLPPSWRESPRDAGEGDTTSLRLLCSVIGGTAPAQWEDITGTTKLAYSNDCASFTTNVSARFWLADCPRTAEAVSFANLLYRELSAVPYMAKFVVFAKMNEAREGRLRCYCMTDDKMDKTLEQHENFSEVARSRDIEVMEGMPLYLECSGNLVPIRKAAQQPRCFSFQAFKDNRLPVSVKVRDSSKDPSGFLSFLRKSTKYEDSQHVLCNLNVTMPPCIKIMGSDERRRTLTPLALRERYSALNEPAMASLSAMERTELKMALIAEQLGLSWAELGRELQFNVDEINKIRVENPNSLLEQSSTLLNLWAAREGKRAKMDSLYVALKSIDRVDIVNMMEGQGGPPAGQQGGWEQDASRSRHHDRDHLSPGITNGYGLVVQEELVSPASMQYSLPSPLGNEPYWQEVSSMECAPIATTEEDTLMEMSEVQVWPSGNSPSIVAVEDSSLECSNADDSEALQNRSLGHSRSGTPGGNGLTPGGLSGSIELLEDQSIGADSDYSAAGMLRLSEGVNISALDRGQRSESSEGAVGHQVTNNIKDGSNGGLGEGGGDGTQLEDSISLISGQQRVYARMSESPGLRRRADPGEDRLSAGSFLSYLQDQGSPGWHSAAEATQNRLASLQCSLPRQAIESMMSSVRATVDSDSKQSCLGQEALIEPVRDMGHSEIIHGHYQGTQPFEKGLGFPHRTTNMRTWEDMRLSGQGDEAEDLPGEQVSEEQFTDEHGNIVTKKIVRKVVRRGKGEEGGQELIIEGLQQDISEPDFDGEQFMSYAVLGRDSKPDVVDVKRGGAQIVKCASLRRVKQ

Target ORF information:

RefSeq Version	XM_021476105.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476105.1

ORF Insert Sequence:

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGCGAGGA GCTGCTGGGA AACGAGGGCG CCAGGTATTT GATGAAGATG 
GAGGACATAA AGGACCATGA TGAGGATTTC CTTTCCCCCA AGAAAGTTCT AGAGAATCAT 
TCTCCTGCTA TCCCGAGGAT CCCGTGTGTG TCTCCTGAGA CTGTCATGCT GAAAGAGCAT 
GAGATGGAGC AGCAGCACAC TCCACTTCCA TTGCCCAAAG AGTACGACGA GGATTCTCTG 
ATTCCCAGCA GTCCTGCAAC TGAGACTTCA GACAATGTCA GTCCGGTTGC CAGTCCCATC 
CACACCGGTT TTCTGGTGAG CTTCATGGTG GATGCTCGCG GCGGCTCGAT GCGCGGCAGC 
AGACACAACG GCCTCCGCGT CATCATTCCT CCTCGAACCT GCGCCGCTCC CACACGCATC 
ACCTGCCGCC TGGTGAAGCC CCAGAAGCTG CCCACACCGC CGCCATTAGT GGAGGGAGAG 
GGTCTCGCCA GCCGCATCAT CTCCCTTGGA CCCGCCAGCA TGCAGTTTTT AGGGCCTGTG 
ATTGTGGAGA TTCCTCATTT TGCGGCTCTC GGTCGGGGAG ATCGGGAACT CGTGGTCTTG 
AGGAGTGAGA ACGGATCGGT CTGGAAGGAA CATCGTAACC GATATGGAGA TGAAGTGCTA 
GAGACCATCC TCAACGGCAT GGATGAAGAT CTGGAGAGTC AGGAGGAGCT CGGGAAGAAA 
CGCATCAGAC GGATCATCTC CACCGACTTC CCGCTCTACT TTGCGGTGGT GTCTCGCATC 
CAGCAGGAGA ACGATCTGAT TGGCCCAGAA GGAGGTTTTC TGTCCAGTAA ACTGGTGCCG 
ATGGTCCAGG CCACCTTTCC AGAGACAGCC GTCACCAAAC GCGTGCGTCT GGGACTGCAG 
GCCCAGCCGG TGCCTGATGA GCTGGTGGCC AAGCTTTTGG GGAACCAGGC CACGTTCAGT 
CCTGTGGTGA CGGTGGAGCC TCGGCGCCGG AAGTTTCACC GTCCCATCGG CCTAAGAATC 
CCACTGCCAC CCTCATGGAG AGAGAGCCCG CGGGACGCCG GAGAGGGAGA CACCACCAGC 
CTGCGCCTGC TCTGCAGCGT CATCGGTGGA ACAGCTCCTG CCCAGTGGGA AGATATAACT 
GGCACCACCA AACTGGCGTA TTCCAATGAC TGTGCCAGTT TCACCACCAA CGTCTCTGCC 
CGATTCTGGC TGGCGGACTG TCCTCGGACT GCGGAGGCCG TGTCCTTTGC CAACCTCCTG 
TACCGCGAGC TGTCGGCCGT GCCGTACATG GCCAAGTTTG TAGTGTTTGC GAAGATGAAT 
GAAGCTCGCG AGGGTCGACT CCGCTGCTAC TGCATGACGG ATGACAAGAT GGACAAAACC 
CTGGAGCAGC ACGAGAACTT CTCGGAAGTG GCTCGCAGCA GAGACATCGA GGTGATGGAG 
GGAATGCCGC TGTATTTGGA GTGTTCAGGA AACTTGGTTC CTATAAGAAA AGCAGCCCAG 
CAGCCTCGCT GTTTCAGCTT CCAGGCCTTT AAAGACAACA GACTGCCTGT GTCCGTCAAG 
GTGAGGGACA GCAGTAAAGA CCCCTCTGGG TTTTTGTCTT TCTTGCGCAA GTCAACCAAA 
TATGAAGACA GCCAACACGT GCTGTGCAAC CTCAACGTGA CCATGCCGCC GTGCATTAAG 
ATTATGGGCA GCGATGAGCG GAGGAGGACT CTGACTCCTC TGGCTCTCAG AGAAAGATAC 
AGCGCTCTGA ATGAACCGGC AATGGCATCA CTGAGCGCCA TGGAAAGGAC GGAGTTAAAG 
ATGGCTTTAA TAGCTGAACA GCTAGGCCTG AGCTGGGCTG AGTTGGGACG GGAACTGCAG 
TTTAATGTAG ATGAAATAAA TAAGATCCGT GTGGAGAATC CCAACTCCCT TTTAGAGCAG 
AGCTCAACGC TGCTCAACCT GTGGGCCGCC CGAGAGGGGA AGAGAGCCAA AATGGACAGC 
CTGTACGTGG CCCTCAAGAG CATCGACCGA GTGGACATTG TGAACATGAT GGAGGGCCAG 
GGAGGACCAC CGGCGGGTCA GCAGGGGGGC TGGGAGCAGG ACGCTTCTAG GTCTAGGCAT 
CACGACCGAG ACCATCTCTC ACCTGGAATC ACAAATGGTT ACGGGCTGGT GGTGCAGGAG 
GAACTAGTGT CACCGGCCTC CATGCAGTAC AGTCTGCCCT CGCCACTGGG CAATGAGCCC 
TACTGGCAGG AGGTTTCCAG CATGGAGTGC GCGCCCATCG CCACCACTGA AGAAGACACG 
CTCATGGAGA TGTCCGAGGT ACAGGTTTGG CCCTCAGGAA ATAGTCCGTC CATTGTGGCT 
GTAGAGGACT CCTCATTGGA GTGCAGCAAT GCGGACGACT CCGAGGCGCT GCAGAACCGG 
AGCCTGGGTC ACTCCAGGAG CGGGACGCCC GGTGGGAACG GACTCACTCC GGGAGGTTTA 
AGTGGCTCCA TCGAGCTCCT GGAGGATCAG TCCATCGGTG CGGATTCAGA CTACAGTGCC 
GCCGGGATGC TGCGTCTGAG CGAAGGGGTC AACATCAGTG CTCTGGACAG AGGTCAGAGG 
TCGGAGAGTT CAGAAGGGGC TGTAGGTCAC CAAGTCACCA ACAACATTAA AGACGGTTCC 
AATGGAGGTT TGGGAGAAGG AGGAGGTGAC GGCACACAGT TAGAAGATAG CATATCTCTC 
ATTTCAGGGC AACAGCGTGT GTATGCTCGA ATGAGCGAGT CGCCTGGGCT GAGAAGACGG 
GCAGATCCTG GCGAAGACAG GTTAAGCGCA GGGTCGTTCC TGTCTTATCT GCAGGATCAG 
GGCTCTCCTG GTTGGCATTC GGCTGCAGAA GCGACTCAGA ACAGATTGGC GTCTTTGCAA 
TGCTCCCTTC CTAGGCAGGC AATAGAGTCT ATGATGTCAT CGGTGCGAGC TACGGTGGAC 
TCTGACTCCA AGCAGTCTTG TTTGGGCCAG GAGGCTCTGA TTGAGCCAGT GCGGGACATG 
GGGCACTCTG AGATCATCCA TGGCCATTAT CAGGGGACTC AGCCGTTTGA GAAAGGACTC 
GGCTTCCCGC ACAGAACAAC CAACATGCGC ACTTGGGAGG ACATGCGTCT GAGCGGACAG 
GGTGATGAGG CTGAGGATCT TCCAGGAGAG CAAGTGAGCG AGGAGCAATT CACAGACGAG 
CATGGCAACA TCGTCACCAA AAAGATTGTG AGGAAAGTGG TCCGGAGAGG AAAAGGGGAG 
GAAGGGGGTC AGGAGCTGAT CATTGAAGGT CTGCAGCAGG ATATCAGTGA GCCTGACTTT 
GATGGAGAAC AGTTCATGAG CTATGCCGTC CTGGGACGTG ACAGCAAGCC GGATGTTGTG 
GATGTGAAGA GAGGAGGTGC TCAGATAGTC AAATGTGCGA GTCTGCGGAG AGTAAAGCAG 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_021476106.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331781.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X8, mRNA.

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CloneID	ODa142598
Clone ID Related Accession (Same CDS sequence)	XM_021476106.1
Accession Version	XM_021476106.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6036bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin 1, erythrocytic a isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGAATTGC ACAGCTAACA TTAGGCGAGG AGCTGCTGGG AAACGAGGGC 
GCCAGGTATT TGATGAAGAT GGAGGACATA AAGGACCATG ATGAGGATTT CCTTTCCCCC 
AAGAAAGTTC TAGAGTACGA GGGAGAGTTG GAGAATCATT CTCCTGCTAT CCCGAGGATC 
CCGTGTGTGT CTCCTGAGAC TGTCATGCTG AAAGAGCATG AGATGGAGCA GCAGCACACT 
CCACTTCCAT TGCCCAAAGA GTACGACGAG GATTCTCTGA TTCCCAGCAG TCCTGCAACT 
GAGACTTCAG ACAATGTCAG TCCGGTTGCC AGTCCCATCC ACACCGGTTT TCTGGTGAGC 
TTCATGGTGG ATGCTCGCGG CGGCTCGATG CGCGGCAGCA GACACAACGG CCTCCGCGTC 
ATCATTCCTC CTCGAACCTG CGCCGCTCCC ACACGCATCA CCTGCCGCCT GGTGAAGCCC 
CAGAAGCTGC CCACACCGCC GCCATTAGTG GAGGGAGAGG GTCTCGCCAG CCGCATCATC 
TCCCTTGGAC CCGCCAGCAT GCAGTTTTTA GGGCCTGTGA TTGTGGAGAT TCCTCATTTT 
GCGGCTCTCG GTCGGGGAGA TCGGGAACTC GTGGTCTTGA GGAGTGAGAA CGGATCGGTC 
TGGAAGGAAC ATCGTAACCG ATATGGAGAT GAAGTGCTAG AGACCATCCT CAACGGCATG 
GATGAAGATC TGGAGAGTCA GGAGGAGCTC GGGAAGAAAC GCATCAGACG GATCATCTCC 
ACCGACTTCC CGCTCTACTT TGCGGTGGTG TCTCGCATCC AGCAGGAGAA CGATCTGATT 
GGCCCAGAAG GAGGTTTTCT GTCCAGTAAA CTGGTGCCGA TGGTCCAGGC CACCTTTCCA 
GAGACAGCCG TCACCAAACG CGTGCGTCTG GGACTGCAGG CCCAGCCGGT GCCTGATGAG 
CTGGTGGCCA AGCTTTTGGG GAACCAGGCC ACGTTCAGTC CTGTGGTGAC GGTGGAGCCT 
CGGCGCCGGA AGTTTCACCG TCCCATCGGC CTAAGAATCC CACTGCCACC CTCATGGAGA 
GAGAGCCCGC GGGACGCCGG AGAGGGAGAC ACCACCAGCC TGCGCCTGCT CTGCAGCGTC 
ATCGGTGGAA CAGCTCCTGC CCAGTGGGAA GATATAACTG GCACCACCAA ACTGGCGTAT 
TCCAATGACT GTGCCAGTTT CACCACCAAC GTCTCTGCCC GATTCTGGCT GGCGGACTGT 
CCTCGGACTG CGGAGGCCGT GTCCTTTGCC AACCTCCTGT ACCGCGAGCT GTCGGCCGTG 
CCGTACATGG CCAAGTTTGT AGTGTTTGCG AAGATGAATG AAGCTCGCGA GGGTCGACTC 
CGCTGCTACT GCATGACGGA TGACAAGATG GACAAAACCC TGGAGCAGCA CGAGAACTTC 
TCGGAAGTGG CTCGCAGCAG AGACATCGAG GTGATGGAGG GAATGCCGCT GTATTTGGAG 
TGTTCAGGAA ACTTGGTTCC TATAAGAAAA GCAGCCCAGC AGCCTCGCTG TTTCAGCTTC 
CAGGCCTTTA AAGACAACAG ACTGCCTGTG TCCGTCAAGG TGAGGGACAG CAGTAAAGAC 
CCCTCTGGGT TTTTGTCTTT CTTGCGCAAG TCAACCAAAT ATGAAGACAG CCAACACGTG 
CTGTGCAACC TCAACGTGAC CATGCCGCCG TGCATTAAGA TTATGGGCAG CGATGAGCGG 
AGGAGGACTC TGACTCCTCT GGCTCTCAGA GAAAGATACA GCGCTCTGAA TGAACCGGCA 
ATGGCATCAC TGAGCGCCAT GGAAAGGACG GAGTTAAAGA TGGCTTTAAT AGCTGAACAG 
CTAGGCCTGA GCTGGGCTGA GTTGGGACGG GAACTGCAGT TTAATGTAGA TGAAATAAAT 
AAGATCCGTG TGGAGAATCC CAACTCCCTT TTAGAGCAGA GCTCAACGCT GCTCAACCTG 
TGGGCCGCCC GAGAGGGGAA GAGAGCCAAA ATGGACAGCC TGTACGTGGC CCTCAAGAGC 
ATCGACCGAG TGGACATTGT GAACATGATG GAGGGCCAGG GAGGACCACC GGCGGGTCAG 
CAGGGGGGCT GGGAGCAGGA CGCTTCTAGG TCTAGGCATC ACGACCGAGA CCATCTCTCA 
CCTGGAATCA CAAATGGTTA CGGGCTGGTG GTGCAGGAGG AACTAGTGTC ACCGGCCTCC 
ATGCAGTACA GTCTGCCCTC GCCACTGGGC AATGAGCCCT ACTGGCAGGA GGTTTCCAGC 
ATGGAGTGCG CGCCCATCGC CACCACTGAA GAAGACACGC TCATGGAGAT GTCCGAGGTA 
CAGGTTTGGC CCTCAGGAAA TAGTCCGTCC ATTGTGGCTG TAGAGGACTC CTCATTGGAG 
TGCAGCAATG CGGACGACTC CGAGGCGCTG CAGAACCGGA GCCTGGGTCA CTCCAGGAGC 
GGGACGCCCG GTGGGAACGG ACTCACTCCG GGAGGTTTAA GTGGCTCCAT CGAGCTCCTG 
GAGGATCAGT CCATCGGTGC GGATTCAGAC TACAGTGCCG CCGGGATGCT GCGTCTGAGC 
GAAGGGGTCA ACATCAGTGC TCTGGACAGA GGTCAGAGGT CGGAGAGTTC AGAAGGGGCT 
GTAGGTCACC AAGTCACCAA CAACATTAAA GACGGTTCCA ATGGAGGTTT GGGAGAAGGA 
GGAGGTGACG GCACACAGTT AGAAGATAGC ATATCTCTCA TTTCAGGGCA ACAGCGTGTG 
TATGCTCGAA TGAGCGAGTC GCCTGGGCTG AGAAGACGGG CAGATCCTGG CGAAGACAGG 
TTAAGCGCAG GGTCGTTCCT GTCTTATCTG CAGGATCAGG GCTCTCCTGG TTGGCATTCG 
GCTGCAGAAG CGACTCAGAA CAGATTGGCG TCTTTGCAAT GCTCCCTTCC TAGGCAGGCA 
ATAGAGTCTA TGATGTCATC GGTGCGAGCT ACGGTGGACT CTGACTCCAA GCAGTCTTGT 
TTGGGCCAGG AGGCTCTGAT TGAGCCAGTG CGGGACATGG GGCACTCTGA GATCATCCAT 
GGCCATTATC AGGGGACTCA GCCGTTTGAG AAAGGACTCG GCTTCCCGCA CAGAACAACC 
AACATGCGCA CTTGGGAGGA CATGCGTCTG AGCGGACAGG GTGATGAGGC TGAGGATCTT 
CCAGGAGAGC AAGTGAGCGA GGAGCAATTC ACAGACGAGC ATGGCAACAT CGTCACCAAA 
AAGATTGTGA GGAAAGTGGT CCGGAGAGGA AAAGGGGAGG AAGGGGGTCA GGAGCTGATC 
ATTGAAGGTC TGCAGCAGGA TATCAGTGAG CCTGACTTTG ATGGAGAACA GTTCATGAGC 
TATGCCGTCC TGGGACGTGA CAGCAAGATC TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331781.1
CDS	222..6257
Translation	MAQAAKHLRKNKDLEALAEQERKEKEEEKSKKRSRSREKKRKAHVVHRWLIDQDNSVSSELPDGQGVWHYDETADAATSFLRAARSGNLEKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLINHGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGLTPLHVSSFMGHLNIVKILMQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGANPNAAGKVGLTPLHVAVHHNNLDVVNLLLSKGGSPHSAARNGYTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPLHLASQEGQPDMVLLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKHGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSNGTSALAIAKRLGYISVIDVLKLVTEETVTMTTTEKHRMSFPETVDEILDVSEDEGIAQLTLGEELLGNEGARYLMKMEDIKDHDEDFLSPKKVLEYEGELENHSPAIPRIPCVSPETVMLKEHEMEQQHTPLPLPKEYDEDSLIPSSPATETSDNVSPVASPIHTGFLVSFMVDARGGSMRGSRHNGLRVIIPPRTCAAPTRITCRLVKPQKLPTPPPLVEGEGLASRIISLGPASMQFLGPVIVEIPHFAALGRGDRELVVLRSENGSVWKEHRNRYGDEVLETILNGMDEDLESQEELGKKRIRRIISTDFPLYFAVVSRIQQENDLIGPEGGFLSSKLVPMVQATFPETAVTKRVRLGLQAQPVPDELVAKLLGNQATFSPVVTVEPRRRKFHRPIGLRIPLPPSWRESPRDAGEGDTTSLRLLCSVIGGTAPAQWEDITGTTKLAYSNDCASFTTNVSARFWLADCPRTAEAVSFANLLYRELSAVPYMAKFVVFAKMNEAREGRLRCYCMTDDKMDKTLEQHENFSEVARSRDIEVMEGMPLYLECSGNLVPIRKAAQQPRCFSFQAFKDNRLPVSVKVRDSSKDPSGFLSFLRKSTKYEDSQHVLCNLNVTMPPCIKIMGSDERRRTLTPLALRERYSALNEPAMASLSAMERTELKMALIAEQLGLSWAELGRELQFNVDEINKIRVENPNSLLEQSSTLLNLWAAREGKRAKMDSLYVALKSIDRVDIVNMMEGQGGPPAGQQGGWEQDASRSRHHDRDHLSPGITNGYGLVVQEELVSPASMQYSLPSPLGNEPYWQEVSSMECAPIATTEEDTLMEMSEVQVWPSGNSPSIVAVEDSSLECSNADDSEALQNRSLGHSRSGTPGGNGLTPGGLSGSIELLEDQSIGADSDYSAAGMLRLSEGVNISALDRGQRSESSEGAVGHQVTNNIKDGSNGGLGEGGGDGTQLEDSISLISGQQRVYARMSESPGLRRRADPGEDRLSAGSFLSYLQDQGSPGWHSAAEATQNRLASLQCSLPRQAIESMMSSVRATVDSDSKQSCLGQEALIEPVRDMGHSEIIHGHYQGTQPFEKGLGFPHRTTNMRTWEDMRLSGQGDEAEDLPGEQVSEEQFTDEHGNIVTKKIVRKVVRRGKGEEGGQELIIEGLQQDISEPDFDGEQFMSYAVLGRDSKIL

Target ORF information:

RefSeq Version	XM_021476106.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476106.1

ORF Insert Sequence:

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGAATTGC ACAGCTAACA TTAGGCGAGG AGCTGCTGGG AAACGAGGGC 
GCCAGGTATT TGATGAAGAT GGAGGACATA AAGGACCATG ATGAGGATTT CCTTTCCCCC 
AAGAAAGTTC TAGAGTACGA GGGAGAGTTG GAGAATCATT CTCCTGCTAT CCCGAGGATC 
CCGTGTGTGT CTCCTGAGAC TGTCATGCTG AAAGAGCATG AGATGGAGCA GCAGCACACT 
CCACTTCCAT TGCCCAAAGA GTACGACGAG GATTCTCTGA TTCCCAGCAG TCCTGCAACT 
GAGACTTCAG ACAATGTCAG TCCGGTTGCC AGTCCCATCC ACACCGGTTT TCTGGTGAGC 
TTCATGGTGG ATGCTCGCGG CGGCTCGATG CGCGGCAGCA GACACAACGG CCTCCGCGTC 
ATCATTCCTC CTCGAACCTG CGCCGCTCCC ACACGCATCA CCTGCCGCCT GGTGAAGCCC 
CAGAAGCTGC CCACACCGCC GCCATTAGTG GAGGGAGAGG GTCTCGCCAG CCGCATCATC 
TCCCTTGGAC CCGCCAGCAT GCAGTTTTTA GGGCCTGTGA TTGTGGAGAT TCCTCATTTT 
GCGGCTCTCG GTCGGGGAGA TCGGGAACTC GTGGTCTTGA GGAGTGAGAA CGGATCGGTC 
TGGAAGGAAC ATCGTAACCG ATATGGAGAT GAAGTGCTAG AGACCATCCT CAACGGCATG 
GATGAAGATC TGGAGAGTCA GGAGGAGCTC GGGAAGAAAC GCATCAGACG GATCATCTCC 
ACCGACTTCC CGCTCTACTT TGCGGTGGTG TCTCGCATCC AGCAGGAGAA CGATCTGATT 
GGCCCAGAAG GAGGTTTTCT GTCCAGTAAA CTGGTGCCGA TGGTCCAGGC CACCTTTCCA 
GAGACAGCCG TCACCAAACG CGTGCGTCTG GGACTGCAGG CCCAGCCGGT GCCTGATGAG 
CTGGTGGCCA AGCTTTTGGG GAACCAGGCC ACGTTCAGTC CTGTGGTGAC GGTGGAGCCT 
CGGCGCCGGA AGTTTCACCG TCCCATCGGC CTAAGAATCC CACTGCCACC CTCATGGAGA 
GAGAGCCCGC GGGACGCCGG AGAGGGAGAC ACCACCAGCC TGCGCCTGCT CTGCAGCGTC 
ATCGGTGGAA CAGCTCCTGC CCAGTGGGAA GATATAACTG GCACCACCAA ACTGGCGTAT 
TCCAATGACT GTGCCAGTTT CACCACCAAC GTCTCTGCCC GATTCTGGCT GGCGGACTGT 
CCTCGGACTG CGGAGGCCGT GTCCTTTGCC AACCTCCTGT ACCGCGAGCT GTCGGCCGTG 
CCGTACATGG CCAAGTTTGT AGTGTTTGCG AAGATGAATG AAGCTCGCGA GGGTCGACTC 
CGCTGCTACT GCATGACGGA TGACAAGATG GACAAAACCC TGGAGCAGCA CGAGAACTTC 
TCGGAAGTGG CTCGCAGCAG AGACATCGAG GTGATGGAGG GAATGCCGCT GTATTTGGAG 
TGTTCAGGAA ACTTGGTTCC TATAAGAAAA GCAGCCCAGC AGCCTCGCTG TTTCAGCTTC 
CAGGCCTTTA AAGACAACAG ACTGCCTGTG TCCGTCAAGG TGAGGGACAG CAGTAAAGAC 
CCCTCTGGGT TTTTGTCTTT CTTGCGCAAG TCAACCAAAT ATGAAGACAG CCAACACGTG 
CTGTGCAACC TCAACGTGAC CATGCCGCCG TGCATTAAGA TTATGGGCAG CGATGAGCGG 
AGGAGGACTC TGACTCCTCT GGCTCTCAGA GAAAGATACA GCGCTCTGAA TGAACCGGCA 
ATGGCATCAC TGAGCGCCAT GGAAAGGACG GAGTTAAAGA TGGCTTTAAT AGCTGAACAG 
CTAGGCCTGA GCTGGGCTGA GTTGGGACGG GAACTGCAGT TTAATGTAGA TGAAATAAAT 
AAGATCCGTG TGGAGAATCC CAACTCCCTT TTAGAGCAGA GCTCAACGCT GCTCAACCTG 
TGGGCCGCCC GAGAGGGGAA GAGAGCCAAA ATGGACAGCC TGTACGTGGC CCTCAAGAGC 
ATCGACCGAG TGGACATTGT GAACATGATG GAGGGCCAGG GAGGACCACC GGCGGGTCAG 
CAGGGGGGCT GGGAGCAGGA CGCTTCTAGG TCTAGGCATC ACGACCGAGA CCATCTCTCA 
CCTGGAATCA CAAATGGTTA CGGGCTGGTG GTGCAGGAGG AACTAGTGTC ACCGGCCTCC 
ATGCAGTACA GTCTGCCCTC GCCACTGGGC AATGAGCCCT ACTGGCAGGA GGTTTCCAGC 
ATGGAGTGCG CGCCCATCGC CACCACTGAA GAAGACACGC TCATGGAGAT GTCCGAGGTA 
CAGGTTTGGC CCTCAGGAAA TAGTCCGTCC ATTGTGGCTG TAGAGGACTC CTCATTGGAG 
TGCAGCAATG CGGACGACTC CGAGGCGCTG CAGAACCGGA GCCTGGGTCA CTCCAGGAGC 
GGGACGCCCG GTGGGAACGG ACTCACTCCG GGAGGTTTAA GTGGCTCCAT CGAGCTCCTG 
GAGGATCAGT CCATCGGTGC GGATTCAGAC TACAGTGCCG CCGGGATGCT GCGTCTGAGC 
GAAGGGGTCA ACATCAGTGC TCTGGACAGA GGTCAGAGGT CGGAGAGTTC AGAAGGGGCT 
GTAGGTCACC AAGTCACCAA CAACATTAAA GACGGTTCCA ATGGAGGTTT GGGAGAAGGA 
GGAGGTGACG GCACACAGTT AGAAGATAGC ATATCTCTCA TTTCAGGGCA ACAGCGTGTG 
TATGCTCGAA TGAGCGAGTC GCCTGGGCTG AGAAGACGGG CAGATCCTGG CGAAGACAGG 
TTAAGCGCAG GGTCGTTCCT GTCTTATCTG CAGGATCAGG GCTCTCCTGG TTGGCATTCG 
GCTGCAGAAG CGACTCAGAA CAGATTGGCG TCTTTGCAAT GCTCCCTTCC TAGGCAGGCA 
ATAGAGTCTA TGATGTCATC GGTGCGAGCT ACGGTGGACT CTGACTCCAA GCAGTCTTGT 
TTGGGCCAGG AGGCTCTGAT TGAGCCAGTG CGGGACATGG GGCACTCTGA GATCATCCAT 
GGCCATTATC AGGGGACTCA GCCGTTTGAG AAAGGACTCG GCTTCCCGCA CAGAACAACC 
AACATGCGCA CTTGGGAGGA CATGCGTCTG AGCGGACAGG GTGATGAGGC TGAGGATCTT 
CCAGGAGAGC AAGTGAGCGA GGAGCAATTC ACAGACGAGC ATGGCAACAT CGTCACCAAA 
AAGATTGTGA GGAAAGTGGT CCGGAGAGGA AAAGGGGAGG AAGGGGGTCA GGAGCTGATC 
ATTGAAGGTC TGCAGCAGGA TATCAGTGAG CCTGACTTTG ATGGAGAACA GTTCATGAGC 
TATGCCGTCC TGGGACGTGA CAGCAAGATC TTGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_021476107.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331782.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X9, mRNA.

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CloneID	ODa142599
Clone ID Related Accession (Same CDS sequence)	XM_021476107.1
Accession Version	XM_021476107.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6015bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin 1, erythrocytic a isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGAATTGC ACAGCTAACA TTAGGCGAGG AGCTGCTGGG AAACGAGGGC 
GCCAGGAATC ATTCTCCTGC TATCCCGAGG ATCCCGTGTG TGTCTCCTGA GACTGTCATG 
CTGAAAGAGC ATGAGATGGA GCAGCAGCAC ACTCCACTTC CATTGCCCAA AGAGTACGAC 
GAGGATTCTC TGATTCCCAG CAGTCCTGCA ACTGAGACTT CAGACAATGT CAGTCCGGTT 
GCCAGTCCCA TCCACACCGG TTTTCTGGTG AGCTTCATGG TGGATGCTCG CGGCGGCTCG 
ATGCGCGGCA GCAGACACAA CGGCCTCCGC GTCATCATTC CTCCTCGAAC CTGCGCCGCT 
CCCACACGCA TCACCTGCCG CCTGGTGAAG CCCCAGAAGC TGCCCACACC GCCGCCATTA 
GTGGAGGGAG AGGGTCTCGC CAGCCGCATC ATCTCCCTTG GACCCGCCAG CATGCAGTTT 
TTAGGGCCTG TGATTGTGGA GATTCCTCAT TTTGCGGCTC TCGGTCGGGG AGATCGGGAA 
CTCGTGGTCT TGAGGAGTGA GAACGGATCG GTCTGGAAGG AACATCGTAA CCGATATGGA 
GATGAAGTGC TAGAGACCAT CCTCAACGGC ATGGATGAAG ATCTGGAGAG TCAGGAGGAG 
CTCGGGAAGA AACGCATCAG ACGGATCATC TCCACCGACT TCCCGCTCTA CTTTGCGGTG 
GTGTCTCGCA TCCAGCAGGA GAACGATCTG ATTGGCCCAG AAGGAGGTTT TCTGTCCAGT 
AAACTGGTGC CGATGGTCCA GGCCACCTTT CCAGAGACAG CCGTCACCAA ACGCGTGCGT 
CTGGGACTGC AGGCCCAGCC GGTGCCTGAT GAGCTGGTGG CCAAGCTTTT GGGGAACCAG 
GCCACGTTCA GTCCTGTGGT GACGGTGGAG CCTCGGCGCC GGAAGTTTCA CCGTCCCATC 
GGCCTAAGAA TCCCACTGCC ACCCTCATGG AGAGAGAGCC CGCGGGACGC CGGAGAGGGA 
GACACCACCA GCCTGCGCCT GCTCTGCAGC GTCATCGGTG GAACAGCTCC TGCCCAGTGG 
GAAGATATAA CTGGCACCAC CAAACTGGCG TATTCCAATG ACTGTGCCAG TTTCACCACC 
AACGTCTCTG CCCGATTCTG GCTGGCGGAC TGTCCTCGGA CTGCGGAGGC CGTGTCCTTT 
GCCAACCTCC TGTACCGCGA GCTGTCGGCC GTGCCGTACA TGGCCAAGTT TGTAGTGTTT 
GCGAAGATGA ATGAAGCTCG CGAGGGTCGA CTCCGCTGCT ACTGCATGAC GGATGACAAG 
ATGGACAAAA CCCTGGAGCA GCACGAGAAC TTCTCGGAAG TGGCTCGCAG CAGAGACATC 
GAGGTGATGG AGGGAATGCC GCTGTATTTG GAGTGTTCAG GAAACTTGGT TCCTATAAGA 
AAAGCAGCCC AGCAGCCTCG CTGTTTCAGC TTCCAGGCCT TTAAAGACAA CAGACTGCCT 
GTGTCCGTCA AGGTGAGGGA CAGCAGTAAA GACCCCTCTG GGTTTTTGTC TTTCTTGCGC 
AAGTCAACCA AATATGAAGA CAGCCAACAC GTGCTGTGCA ACCTCAACGT GACCATGCCG 
CCGTGCATTA AGATTATGGG CAGCGATGAG CGGAGGAGGA CTCTGACTCC TCTGGCTCTC 
AGAGAAAGAT ACAGCGCTCT GAATGAACCG GCAATGGCAT CACTGAGCGC CATGGAAAGG 
ACGGAGTTAA AGATGGCTTT AATAGCTGAA CAGCTAGGCC TGAGCTGGGC TGAGTTGGGA 
CGGGAACTGC AGTTTAATGT AGATGAAATA AATAAGATCC GTGTGGAGAA TCCCAACTCC 
CTTTTAGAGC AGAGCTCAAC GCTGCTCAAC CTGTGGGCCG CCCGAGAGGG GAAGAGAGCC 
AAAATGGACA GCCTGTACGT GGCCCTCAAG AGCATCGACC GAGTGGACAT TGTGAACATG 
ATGGAGGGCC AGGGAGGACC ACCGGCGGGT CAGCAGGGGG GCTGGGAGCA GGACGCTTCT 
AGGTCTAGGC ATCACGACCG AGACCATCTC TCACCTGGAA TCACAAATGG TTACGGGCTG 
GTGGTGCAGG AGGAACTAGT GTCACCGGCC TCCATGCAGT ACAGTCTGCC CTCGCCACTG 
GGCAATGAGC CCTACTGGCA GGAGGTTTCC AGCATGGAGT GCGCGCCCAT CGCCACCACT 
GAAGAAGACA CGCTCATGGA GATGTCCGAG GTACAGGTTT GGCCCTCAGG AAATAGTCCG 
TCCATTGTGG CTGTAGAGGA CTCCTCATTG GAGTGCAGCA ATGCGGACGA CTCCGAGGCG 
CTGCAGAACC GGAGCCTGGG TCACTCCAGG AGCGGGACGC CCGGTGGGAA CGGACTCACT 
CCGGGAGGTT TAAGTGGCTC CATCGAGCTC CTGGAGGATC AGTCCATCGG TGCGGATTCA 
GACTACAGTG CCGCCGGGAT GCTGCGTCTG AGCGAAGGGG TCAACATCAG TGCTCTGGAC 
AGAGGTCAGA GGTCGGAGAG TTCAGAAGGG GCTGTAGGTC ACCAAGTCAC CAACAACATT 
AAAGACGGTT CCAATGGAGG TTTGGGAGAA GGAGGAGGTG ACGGCACACA GTTAGAAGAT 
AGCATATCTC TCATTTCAGG GCAACAGCGT GTGTATGCTC GAATGAGCGA GTCGCCTGGG 
CTGAGAAGAC GGGCAGATCC TGGCGAAGAC AGGTTAAGCG CAGGGTCGTT CCTGTCTTAT 
CTGCAGGATC AGGGCTCTCC TGGTTGGCAT TCGGCTGCAG AAGCGACTCA GAACAGATTG 
GCGTCTTTGC AATGCTCCCT TCCTAGGCAG GCAATAGAGT CTATGATGTC ATCGGTGCGA 
GCTACGGTGG ACTCTGACTC CAAGCAGTCT TGTTTGGGCC AGGAGGCTCT GATTGAGCCA 
GTGCGGGACA TGGGGCACTC TGAGATCATC CATGGCCATT ATCAGGGGAC TCAGCCGTTT 
GAGAAAGGAC TCGGCTTCCC GCACAGAACA ACCAACATGC GCACTTGGGA GGACATGCGT 
CTGAGCGGAC AGGGTGATGA GGCTGAGGAT CTTCCAGGAG AGCAAGTGAG CGAGGAGCAA 
TTCACAGACG AGCATGGCAA CATCGTCACC AAAAAGATTG TGAGGAAAGT GGTCCGGAGA 
GGAAAAGGGG AGGAAGGGGG TCAGGAGCTG ATCATTGAAG GTCTGCAGCA GGATATCAGT 
GAGCCTGACT TTGATGGAGA ACAGTTCATG AGCTATGCCG TCCTGGGACG TGACAGCAAG 
CCGGATGTTG TGGATGTGAA GAGAGGAGGT GCTCAGATAG TCAAATGTGC GAGTCTGCGG 
AGAGTAAAGC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331782.1
CDS	222..6236
Translation	MAQAAKHLRKNKDLEALAEQERKEKEEEKSKKRSRSREKKRKAHVVHRWLIDQDNSVSSELPDGQGVWHYDETADAATSFLRAARSGNLEKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLINHGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGLTPLHVSSFMGHLNIVKILMQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGANPNAAGKVGLTPLHVAVHHNNLDVVNLLLSKGGSPHSAARNGYTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPLHLASQEGQPDMVLLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKHGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSNGTSALAIAKRLGYISVIDVLKLVTEETVTMTTTEKHRMSFPETVDEILDVSEDEGIAQLTLGEELLGNEGARNHSPAIPRIPCVSPETVMLKEHEMEQQHTPLPLPKEYDEDSLIPSSPATETSDNVSPVASPIHTGFLVSFMVDARGGSMRGSRHNGLRVIIPPRTCAAPTRITCRLVKPQKLPTPPPLVEGEGLASRIISLGPASMQFLGPVIVEIPHFAALGRGDRELVVLRSENGSVWKEHRNRYGDEVLETILNGMDEDLESQEELGKKRIRRIISTDFPLYFAVVSRIQQENDLIGPEGGFLSSKLVPMVQATFPETAVTKRVRLGLQAQPVPDELVAKLLGNQATFSPVVTVEPRRRKFHRPIGLRIPLPPSWRESPRDAGEGDTTSLRLLCSVIGGTAPAQWEDITGTTKLAYSNDCASFTTNVSARFWLADCPRTAEAVSFANLLYRELSAVPYMAKFVVFAKMNEAREGRLRCYCMTDDKMDKTLEQHENFSEVARSRDIEVMEGMPLYLECSGNLVPIRKAAQQPRCFSFQAFKDNRLPVSVKVRDSSKDPSGFLSFLRKSTKYEDSQHVLCNLNVTMPPCIKIMGSDERRRTLTPLALRERYSALNEPAMASLSAMERTELKMALIAEQLGLSWAELGRELQFNVDEINKIRVENPNSLLEQSSTLLNLWAAREGKRAKMDSLYVALKSIDRVDIVNMMEGQGGPPAGQQGGWEQDASRSRHHDRDHLSPGITNGYGLVVQEELVSPASMQYSLPSPLGNEPYWQEVSSMECAPIATTEEDTLMEMSEVQVWPSGNSPSIVAVEDSSLECSNADDSEALQNRSLGHSRSGTPGGNGLTPGGLSGSIELLEDQSIGADSDYSAAGMLRLSEGVNISALDRGQRSESSEGAVGHQVTNNIKDGSNGGLGEGGGDGTQLEDSISLISGQQRVYARMSESPGLRRRADPGEDRLSAGSFLSYLQDQGSPGWHSAAEATQNRLASLQCSLPRQAIESMMSSVRATVDSDSKQSCLGQEALIEPVRDMGHSEIIHGHYQGTQPFEKGLGFPHRTTNMRTWEDMRLSGQGDEAEDLPGEQVSEEQFTDEHGNIVTKKIVRKVVRRGKGEEGGQELIIEGLQQDISEPDFDGEQFMSYAVLGRDSKPDVVDVKRGGAQIVKCASLRRVKQ

Target ORF information:

RefSeq Version	XM_021476107.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476107.1

ORF Insert Sequence:

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGAATTGC ACAGCTAACA TTAGGCGAGG AGCTGCTGGG AAACGAGGGC 
GCCAGGAATC ATTCTCCTGC TATCCCGAGG ATCCCGTGTG TGTCTCCTGA GACTGTCATG 
CTGAAAGAGC ATGAGATGGA GCAGCAGCAC ACTCCACTTC CATTGCCCAA AGAGTACGAC 
GAGGATTCTC TGATTCCCAG CAGTCCTGCA ACTGAGACTT CAGACAATGT CAGTCCGGTT 
GCCAGTCCCA TCCACACCGG TTTTCTGGTG AGCTTCATGG TGGATGCTCG CGGCGGCTCG 
ATGCGCGGCA GCAGACACAA CGGCCTCCGC GTCATCATTC CTCCTCGAAC CTGCGCCGCT 
CCCACACGCA TCACCTGCCG CCTGGTGAAG CCCCAGAAGC TGCCCACACC GCCGCCATTA 
GTGGAGGGAG AGGGTCTCGC CAGCCGCATC ATCTCCCTTG GACCCGCCAG CATGCAGTTT 
TTAGGGCCTG TGATTGTGGA GATTCCTCAT TTTGCGGCTC TCGGTCGGGG AGATCGGGAA 
CTCGTGGTCT TGAGGAGTGA GAACGGATCG GTCTGGAAGG AACATCGTAA CCGATATGGA 
GATGAAGTGC TAGAGACCAT CCTCAACGGC ATGGATGAAG ATCTGGAGAG TCAGGAGGAG 
CTCGGGAAGA AACGCATCAG ACGGATCATC TCCACCGACT TCCCGCTCTA CTTTGCGGTG 
GTGTCTCGCA TCCAGCAGGA GAACGATCTG ATTGGCCCAG AAGGAGGTTT TCTGTCCAGT 
AAACTGGTGC CGATGGTCCA GGCCACCTTT CCAGAGACAG CCGTCACCAA ACGCGTGCGT 
CTGGGACTGC AGGCCCAGCC GGTGCCTGAT GAGCTGGTGG CCAAGCTTTT GGGGAACCAG 
GCCACGTTCA GTCCTGTGGT GACGGTGGAG CCTCGGCGCC GGAAGTTTCA CCGTCCCATC 
GGCCTAAGAA TCCCACTGCC ACCCTCATGG AGAGAGAGCC CGCGGGACGC CGGAGAGGGA 
GACACCACCA GCCTGCGCCT GCTCTGCAGC GTCATCGGTG GAACAGCTCC TGCCCAGTGG 
GAAGATATAA CTGGCACCAC CAAACTGGCG TATTCCAATG ACTGTGCCAG TTTCACCACC 
AACGTCTCTG CCCGATTCTG GCTGGCGGAC TGTCCTCGGA CTGCGGAGGC CGTGTCCTTT 
GCCAACCTCC TGTACCGCGA GCTGTCGGCC GTGCCGTACA TGGCCAAGTT TGTAGTGTTT 
GCGAAGATGA ATGAAGCTCG CGAGGGTCGA CTCCGCTGCT ACTGCATGAC GGATGACAAG 
ATGGACAAAA CCCTGGAGCA GCACGAGAAC TTCTCGGAAG TGGCTCGCAG CAGAGACATC 
GAGGTGATGG AGGGAATGCC GCTGTATTTG GAGTGTTCAG GAAACTTGGT TCCTATAAGA 
AAAGCAGCCC AGCAGCCTCG CTGTTTCAGC TTCCAGGCCT TTAAAGACAA CAGACTGCCT 
GTGTCCGTCA AGGTGAGGGA CAGCAGTAAA GACCCCTCTG GGTTTTTGTC TTTCTTGCGC 
AAGTCAACCA AATATGAAGA CAGCCAACAC GTGCTGTGCA ACCTCAACGT GACCATGCCG 
CCGTGCATTA AGATTATGGG CAGCGATGAG CGGAGGAGGA CTCTGACTCC TCTGGCTCTC 
AGAGAAAGAT ACAGCGCTCT GAATGAACCG GCAATGGCAT CACTGAGCGC CATGGAAAGG 
ACGGAGTTAA AGATGGCTTT AATAGCTGAA CAGCTAGGCC TGAGCTGGGC TGAGTTGGGA 
CGGGAACTGC AGTTTAATGT AGATGAAATA AATAAGATCC GTGTGGAGAA TCCCAACTCC 
CTTTTAGAGC AGAGCTCAAC GCTGCTCAAC CTGTGGGCCG CCCGAGAGGG GAAGAGAGCC 
AAAATGGACA GCCTGTACGT GGCCCTCAAG AGCATCGACC GAGTGGACAT TGTGAACATG 
ATGGAGGGCC AGGGAGGACC ACCGGCGGGT CAGCAGGGGG GCTGGGAGCA GGACGCTTCT 
AGGTCTAGGC ATCACGACCG AGACCATCTC TCACCTGGAA TCACAAATGG TTACGGGCTG 
GTGGTGCAGG AGGAACTAGT GTCACCGGCC TCCATGCAGT ACAGTCTGCC CTCGCCACTG 
GGCAATGAGC CCTACTGGCA GGAGGTTTCC AGCATGGAGT GCGCGCCCAT CGCCACCACT 
GAAGAAGACA CGCTCATGGA GATGTCCGAG GTACAGGTTT GGCCCTCAGG AAATAGTCCG 
TCCATTGTGG CTGTAGAGGA CTCCTCATTG GAGTGCAGCA ATGCGGACGA CTCCGAGGCG 
CTGCAGAACC GGAGCCTGGG TCACTCCAGG AGCGGGACGC CCGGTGGGAA CGGACTCACT 
CCGGGAGGTT TAAGTGGCTC CATCGAGCTC CTGGAGGATC AGTCCATCGG TGCGGATTCA 
GACTACAGTG CCGCCGGGAT GCTGCGTCTG AGCGAAGGGG TCAACATCAG TGCTCTGGAC 
AGAGGTCAGA GGTCGGAGAG TTCAGAAGGG GCTGTAGGTC ACCAAGTCAC CAACAACATT 
AAAGACGGTT CCAATGGAGG TTTGGGAGAA GGAGGAGGTG ACGGCACACA GTTAGAAGAT 
AGCATATCTC TCATTTCAGG GCAACAGCGT GTGTATGCTC GAATGAGCGA GTCGCCTGGG 
CTGAGAAGAC GGGCAGATCC TGGCGAAGAC AGGTTAAGCG CAGGGTCGTT CCTGTCTTAT 
CTGCAGGATC AGGGCTCTCC TGGTTGGCAT TCGGCTGCAG AAGCGACTCA GAACAGATTG 
GCGTCTTTGC AATGCTCCCT TCCTAGGCAG GCAATAGAGT CTATGATGTC ATCGGTGCGA 
GCTACGGTGG ACTCTGACTC CAAGCAGTCT TGTTTGGGCC AGGAGGCTCT GATTGAGCCA 
GTGCGGGACA TGGGGCACTC TGAGATCATC CATGGCCATT ATCAGGGGAC TCAGCCGTTT 
GAGAAAGGAC TCGGCTTCCC GCACAGAACA ACCAACATGC GCACTTGGGA GGACATGCGT 
CTGAGCGGAC AGGGTGATGA GGCTGAGGAT CTTCCAGGAG AGCAAGTGAG CGAGGAGCAA 
TTCACAGACG AGCATGGCAA CATCGTCACC AAAAAGATTG TGAGGAAAGT GGTCCGGAGA 
GGAAAAGGGG AGGAAGGGGG TCAGGAGCTG ATCATTGAAG GTCTGCAGCA GGATATCAGT 
GAGCCTGACT TTGATGGAGA ACAGTTCATG AGCTATGCCG TCCTGGGACG TGACAGCAAG 
CCGGATGTTG TGGATGTGAA GAGAGGAGGT GCTCAGATAG TCAAATGTGC GAGTCTGCGG 
AGAGTAAAGC AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_021476108.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331783.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X10, mRNA.

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CloneID	ODa142600
Clone ID Related Accession (Same CDS sequence)	XM_021476108.1
Accession Version	XM_021476108.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6012bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin 1, erythrocytic a isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGAATTGC ACAGCTAACA TTAGGCGAGG AGCTGCTGGG AAACGAGGGC 
GCCAGGAATC ATTCTCCTGC TATCCCGAGG ATCCCGTGTG TGTCTCCTGA GACTGTCATG 
CTGAAAGAGC ATGAGATGGA GCAGCACACT CCACTTCCAT TGCCCAAAGA GTACGACGAG 
GATTCTCTGA TTCCCAGCAG TCCTGCAACT GAGACTTCAG ACAATGTCAG TCCGGTTGCC 
AGTCCCATCC ACACCGGTTT TCTGGTGAGC TTCATGGTGG ATGCTCGCGG CGGCTCGATG 
CGCGGCAGCA GACACAACGG CCTCCGCGTC ATCATTCCTC CTCGAACCTG CGCCGCTCCC 
ACACGCATCA CCTGCCGCCT GGTGAAGCCC CAGAAGCTGC CCACACCGCC GCCATTAGTG 
GAGGGAGAGG GTCTCGCCAG CCGCATCATC TCCCTTGGAC CCGCCAGCAT GCAGTTTTTA 
GGGCCTGTGA TTGTGGAGAT TCCTCATTTT GCGGCTCTCG GTCGGGGAGA TCGGGAACTC 
GTGGTCTTGA GGAGTGAGAA CGGATCGGTC TGGAAGGAAC ATCGTAACCG ATATGGAGAT 
GAAGTGCTAG AGACCATCCT CAACGGCATG GATGAAGATC TGGAGAGTCA GGAGGAGCTC 
GGGAAGAAAC GCATCAGACG GATCATCTCC ACCGACTTCC CGCTCTACTT TGCGGTGGTG 
TCTCGCATCC AGCAGGAGAA CGATCTGATT GGCCCAGAAG GAGGTTTTCT GTCCAGTAAA 
CTGGTGCCGA TGGTCCAGGC CACCTTTCCA GAGACAGCCG TCACCAAACG CGTGCGTCTG 
GGACTGCAGG CCCAGCCGGT GCCTGATGAG CTGGTGGCCA AGCTTTTGGG GAACCAGGCC 
ACGTTCAGTC CTGTGGTGAC GGTGGAGCCT CGGCGCCGGA AGTTTCACCG TCCCATCGGC 
CTAAGAATCC CACTGCCACC CTCATGGAGA GAGAGCCCGC GGGACGCCGG AGAGGGAGAC 
ACCACCAGCC TGCGCCTGCT CTGCAGCGTC ATCGGTGGAA CAGCTCCTGC CCAGTGGGAA 
GATATAACTG GCACCACCAA ACTGGCGTAT TCCAATGACT GTGCCAGTTT CACCACCAAC 
GTCTCTGCCC GATTCTGGCT GGCGGACTGT CCTCGGACTG CGGAGGCCGT GTCCTTTGCC 
AACCTCCTGT ACCGCGAGCT GTCGGCCGTG CCGTACATGG CCAAGTTTGT AGTGTTTGCG 
AAGATGAATG AAGCTCGCGA GGGTCGACTC CGCTGCTACT GCATGACGGA TGACAAGATG 
GACAAAACCC TGGAGCAGCA CGAGAACTTC TCGGAAGTGG CTCGCAGCAG AGACATCGAG 
GTGATGGAGG GAATGCCGCT GTATTTGGAG TGTTCAGGAA ACTTGGTTCC TATAAGAAAA 
GCAGCCCAGC AGCCTCGCTG TTTCAGCTTC CAGGCCTTTA AAGACAACAG ACTGCCTGTG 
TCCGTCAAGG TGAGGGACAG CAGTAAAGAC CCCTCTGGGT TTTTGTCTTT CTTGCGCAAG 
TCAACCAAAT ATGAAGACAG CCAACACGTG CTGTGCAACC TCAACGTGAC CATGCCGCCG 
TGCATTAAGA TTATGGGCAG CGATGAGCGG AGGAGGACTC TGACTCCTCT GGCTCTCAGA 
GAAAGATACA GCGCTCTGAA TGAACCGGCA ATGGCATCAC TGAGCGCCAT GGAAAGGACG 
GAGTTAAAGA TGGCTTTAAT AGCTGAACAG CTAGGCCTGA GCTGGGCTGA GTTGGGACGG 
GAACTGCAGT TTAATGTAGA TGAAATAAAT AAGATCCGTG TGGAGAATCC CAACTCCCTT 
TTAGAGCAGA GCTCAACGCT GCTCAACCTG TGGGCCGCCC GAGAGGGGAA GAGAGCCAAA 
ATGGACAGCC TGTACGTGGC CCTCAAGAGC ATCGACCGAG TGGACATTGT GAACATGATG 
GAGGGCCAGG GAGGACCACC GGCGGGTCAG CAGGGGGGCT GGGAGCAGGA CGCTTCTAGG 
TCTAGGCATC ACGACCGAGA CCATCTCTCA CCTGGAATCA CAAATGGTTA CGGGCTGGTG 
GTGCAGGAGG AACTAGTGTC ACCGGCCTCC ATGCAGTACA GTCTGCCCTC GCCACTGGGC 
AATGAGCCCT ACTGGCAGGA GGTTTCCAGC ATGGAGTGCG CGCCCATCGC CACCACTGAA 
GAAGACACGC TCATGGAGAT GTCCGAGGTA CAGGTTTGGC CCTCAGGAAA TAGTCCGTCC 
ATTGTGGCTG TAGAGGACTC CTCATTGGAG TGCAGCAATG CGGACGACTC CGAGGCGCTG 
CAGAACCGGA GCCTGGGTCA CTCCAGGAGC GGGACGCCCG GTGGGAACGG ACTCACTCCG 
GGAGGTTTAA GTGGCTCCAT CGAGCTCCTG GAGGATCAGT CCATCGGTGC GGATTCAGAC 
TACAGTGCCG CCGGGATGCT GCGTCTGAGC GAAGGGGTCA ACATCAGTGC TCTGGACAGA 
GGTCAGAGGT CGGAGAGTTC AGAAGGGGCT GTAGGTCACC AAGTCACCAA CAACATTAAA 
GACGGTTCCA ATGGAGGTTT GGGAGAAGGA GGAGGTGACG GCACACAGTT AGAAGATAGC 
ATATCTCTCA TTTCAGGGCA ACAGCGTGTG TATGCTCGAA TGAGCGAGTC GCCTGGGCTG 
AGAAGACGGG CAGATCCTGG CGAAGACAGG TTAAGCGCAG GGTCGTTCCT GTCTTATCTG 
CAGGATCAGG GCTCTCCTGG TTGGCATTCG GCTGCAGAAG CGACTCAGAA CAGATTGGCG 
TCTTTGCAAT GCTCCCTTCC TAGGCAGGCA ATAGAGTCTA TGATGTCATC GGTGCGAGCT 
ACGGTGGACT CTGACTCCAA GCAGTCTTGT TTGGGCCAGG AGGCTCTGAT TGAGCCAGTG 
CGGGACATGG GGCACTCTGA GATCATCCAT GGCCATTATC AGGGGACTCA GCCGTTTGAG 
AAAGGACTCG GCTTCCCGCA CAGAACAACC AACATGCGCA CTTGGGAGGA CATGCGTCTG 
AGCGGACAGG GTGATGAGGC TGAGGATCTT CCAGGAGAGC AAGTGAGCGA GGAGCAATTC 
ACAGACGAGC ATGGCAACAT CGTCACCAAA AAGATTGTGA GGAAAGTGGT CCGGAGAGGA 
AAAGGGGAGG AAGGGGGTCA GGAGCTGATC ATTGAAGGTC TGCAGCAGGA TATCAGTGAG 
CCTGACTTTG ATGGAGAACA GTTCATGAGC TATGCCGTCC TGGGACGTGA CAGCAAGCCG 
GATGTTGTGG ATGTGAAGAG AGGAGGTGCT CAGATAGTCA AATGTGCGAG TCTGCGGAGA 
GTAAAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331783.1
CDS	222..6233
Translation	MAQAAKHLRKNKDLEALAEQERKEKEEEKSKKRSRSREKKRKAHVVHRWLIDQDNSVSSELPDGQGVWHYDETADAATSFLRAARSGNLEKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLINHGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGLTPLHVSSFMGHLNIVKILMQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGANPNAAGKVGLTPLHVAVHHNNLDVVNLLLSKGGSPHSAARNGYTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPLHLASQEGQPDMVLLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKHGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSNGTSALAIAKRLGYISVIDVLKLVTEETVTMTTTEKHRMSFPETVDEILDVSEDEGIAQLTLGEELLGNEGARNHSPAIPRIPCVSPETVMLKEHEMEQHTPLPLPKEYDEDSLIPSSPATETSDNVSPVASPIHTGFLVSFMVDARGGSMRGSRHNGLRVIIPPRTCAAPTRITCRLVKPQKLPTPPPLVEGEGLASRIISLGPASMQFLGPVIVEIPHFAALGRGDRELVVLRSENGSVWKEHRNRYGDEVLETILNGMDEDLESQEELGKKRIRRIISTDFPLYFAVVSRIQQENDLIGPEGGFLSSKLVPMVQATFPETAVTKRVRLGLQAQPVPDELVAKLLGNQATFSPVVTVEPRRRKFHRPIGLRIPLPPSWRESPRDAGEGDTTSLRLLCSVIGGTAPAQWEDITGTTKLAYSNDCASFTTNVSARFWLADCPRTAEAVSFANLLYRELSAVPYMAKFVVFAKMNEAREGRLRCYCMTDDKMDKTLEQHENFSEVARSRDIEVMEGMPLYLECSGNLVPIRKAAQQPRCFSFQAFKDNRLPVSVKVRDSSKDPSGFLSFLRKSTKYEDSQHVLCNLNVTMPPCIKIMGSDERRRTLTPLALRERYSALNEPAMASLSAMERTELKMALIAEQLGLSWAELGRELQFNVDEINKIRVENPNSLLEQSSTLLNLWAAREGKRAKMDSLYVALKSIDRVDIVNMMEGQGGPPAGQQGGWEQDASRSRHHDRDHLSPGITNGYGLVVQEELVSPASMQYSLPSPLGNEPYWQEVSSMECAPIATTEEDTLMEMSEVQVWPSGNSPSIVAVEDSSLECSNADDSEALQNRSLGHSRSGTPGGNGLTPGGLSGSIELLEDQSIGADSDYSAAGMLRLSEGVNISALDRGQRSESSEGAVGHQVTNNIKDGSNGGLGEGGGDGTQLEDSISLISGQQRVYARMSESPGLRRRADPGEDRLSAGSFLSYLQDQGSPGWHSAAEATQNRLASLQCSLPRQAIESMMSSVRATVDSDSKQSCLGQEALIEPVRDMGHSEIIHGHYQGTQPFEKGLGFPHRTTNMRTWEDMRLSGQGDEAEDLPGEQVSEEQFTDEHGNIVTKKIVRKVVRRGKGEEGGQELIIEGLQQDISEPDFDGEQFMSYAVLGRDSKPDVVDVKRGGAQIVKCASLRRVKQ

Target ORF information:

RefSeq Version	XM_021476108.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476108.1

ORF Insert Sequence:

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCCC ACGTCGTTCA TCGCTGGCTA ATCGATCAGG ACAATAGTGT GAGCTCTGAG 
CTGCCGGATG GCCAGGGAGT GTGGCACTAT GATGAAACAG CTGACGCTGC CACCAGTTTC 
CTCCGCGCAG CTCGCTCAGG TAATCTGGAA AAGGCTCTAG ACCACATCAA GAACGGCATC 
GACATCAACA CAGCCAATCA GAATGGACTC AACGGGCTGC ATTTGGCCTC CAAGGAAGGC 
CATGTTAAAA TGGTGCTGGA GCTGCTTCAC CATGGTATTG TACTTGAGAC GACAACGAAG 
AAAGGGAACA CTGCTCTTCA TATAGCGGCG TTGGCTGGCC AGGAGCAGGT GGTCACTGAA 
CTGGTCAACT ATGGAGCAAA CGTCAACGCT CAGTCTCAGA AGGGCTTCAC GCCGCTCTAC 
ATGGCAGCAC AAGAGAATCA TCTGGAGGTT GTGAAGTTTC TGCTGGATAA CGGAGCCAAT 
CAGACCATTC CAACTGAGGA TGGCTTTACC CCTCTTGCTG TGGCTCTTCA GCAGGGCCAT 
GAGAATGTGG TGGCCCTGCT CATCAACCAC GGTACTAAGG GCAAGGTCCG CCTTCCCGCA 
CTGCACATCG CTGCCCGAAA CGACGACACC CGCACTGCCG CTGTGCTCTT ACAGAACGAC 
CCCAATCCTG ACGTCCTGAG CAAGACAGGC TTCACGCCGC TCCACATCGC AGCACATTAT 
GAAAACCTGA ATGTTGCTCA GCTTCTTCTG AACCGAGGAG CTGATGTGAA CTTCACACCT 
AAGAACGGGA TCACACCGCT GCACATCGCA TCCAGGAGAG GGAACGTCAT CATGGTCAGA 
CTTCTGCTGG ACCGAGGAGC AAAGATCGAT GCCAAGACAA AAGATGAGTT GACTCCTCTT 
CACTGTGCTG CCAGAAATGG CCATGTGAGA ATCATCGAGA TCCTTCTGGA CCAAGGAGCC 
CCAATACAGG CCAAGACCAA AAATGGCCTG TCGCCGATCC ACATGGCAGC CCAGGGCGAT 
CACTTGGACT GTATCCGACA GCTCTTACAG TACAACGCAG AGATCGATGA CATCACTCTG 
GACCACCTGA CACCTCTGCA TGTGGCGGCT CACTGCGGGC ATCACCGCGT GGCCAAGGTG 
CTCCTGGATA AAGGAGCCAA ACCAAACACC CGCGCTCTGA ATGGATTCAC GCCGCTCCAC 
ATTGCCTGTA AGAAGAACCA CATGCGTGTA ATGGACCTGC TATTAAAGCA TTCTGCATCC 
CTGGAAGCTG TAACCGAGTC AGGCTTGACC CCGCTGCATG TGTCCTCCTT CATGGGTCAT 
CTGAATATAG TCAAGATACT GATGCAAAAA GGAGCTTCGC CGAACGCCTC AAATGTGAAA 
GTGGAAACCC CTCTGCATAT GGCATCTCGA GCTGGACACT GTGAAGTTGC TGAATTCCTA 
TTGCAAAATG CAGCTCCAGT TGATGCCAAA GCCAAGGATG ACCAGACGCC GCTACACTGT 
GCCTCCCGCA TGGGCCACAA CGAGATGGTG AAGTTGCTTC TGGAGCACAA GGCCAACTCA 
AACTCCACCA CCACAGCAGG ACACACACCG CTGCACATCG CGGCCCGCGA GGGACACACA 
CAAACTGCCA GCATCCTCCT GGACATGAAC GCCCAGCTCA CCAAAATGAC CAAGAAAGGC 
TTCACTCCTC TTCATGTTGC AGCCAAATAC GGAAAGGTGG ACGTGGCAGT TCTGCTTCTG 
GAGAGGGGAG CAAATCCAAA CGCTGCTGGA AAAGTTGGAC TCACGCCGCT GCATGTGGCC 
GTCCATCATA ACAATCTAGA TGTGGTCAAT CTTTTACTCA GCAAAGGAGG ATCACCACAC 
AGCGCCGCTC GGAATGGCTA TACTCCGCTC CACATTGCTT CCAAACAGAA CCAGGCGGAG 
GTGGCGAGCA GTTTACTGCA GCACGGGGCG TCTGCCAATG CTGAGTCTCT GCAAGGGGTC 
ACACCTCTAC ACCTCGCCTC TCAAGAGGGT CAACCTGACA TGGTGTTACT GCTCATCTCC 
AAGCAGGCCA ATGTAAACCT TGGCAATAAG AGCGGTCTGA CTCCTCTTCA TCTTGTGGCT 
CAGGAAGGGC ATGTTGGGAT TGCTGATATA CTAGTGAAAC ATGGAGCATC TGTTTATGCT 
GCCACACGGA TGGGTTACAC TCCGCTTCAC GTGGCTTGTC ATTATGGGAA TATCAAGATG 
GTGAAGTTTC TCTTACAGCA GCAGGCCAAC GTAAACTGCA AGACCAGGAT GAGCTACACG 
CCGCTACATC AGGCTGCTCA GCAGGGTCAT ACGGACATCG TGACGCTTCT GCTGAAACAC 
GGAGCACAAC CGAATGAAGT TACATCTAAC GGCACCTCTG CTCTTGCCAT CGCCAAGCGG 
CTTGGATACA TTTCAGTCAT CGATGTGCTC AAACTGGTCA CTGAAGAGAC GGTCACTATG 
ACCACCACTG AAAAACATCG CATGAGTTTC CCTGAAACCG TTGATGAGAT CCTGGATGTG 
TCTGAAGATG AAGGAATTGC ACAGCTAACA TTAGGCGAGG AGCTGCTGGG AAACGAGGGC 
GCCAGGAATC ATTCTCCTGC TATCCCGAGG ATCCCGTGTG TGTCTCCTGA GACTGTCATG 
CTGAAAGAGC ATGAGATGGA GCAGCACACT CCACTTCCAT TGCCCAAAGA GTACGACGAG 
GATTCTCTGA TTCCCAGCAG TCCTGCAACT GAGACTTCAG ACAATGTCAG TCCGGTTGCC 
AGTCCCATCC ACACCGGTTT TCTGGTGAGC TTCATGGTGG ATGCTCGCGG CGGCTCGATG 
CGCGGCAGCA GACACAACGG CCTCCGCGTC ATCATTCCTC CTCGAACCTG CGCCGCTCCC 
ACACGCATCA CCTGCCGCCT GGTGAAGCCC CAGAAGCTGC CCACACCGCC GCCATTAGTG 
GAGGGAGAGG GTCTCGCCAG CCGCATCATC TCCCTTGGAC CCGCCAGCAT GCAGTTTTTA 
GGGCCTGTGA TTGTGGAGAT TCCTCATTTT GCGGCTCTCG GTCGGGGAGA TCGGGAACTC 
GTGGTCTTGA GGAGTGAGAA CGGATCGGTC TGGAAGGAAC ATCGTAACCG ATATGGAGAT 
GAAGTGCTAG AGACCATCCT CAACGGCATG GATGAAGATC TGGAGAGTCA GGAGGAGCTC 
GGGAAGAAAC GCATCAGACG GATCATCTCC ACCGACTTCC CGCTCTACTT TGCGGTGGTG 
TCTCGCATCC AGCAGGAGAA CGATCTGATT GGCCCAGAAG GAGGTTTTCT GTCCAGTAAA 
CTGGTGCCGA TGGTCCAGGC CACCTTTCCA GAGACAGCCG TCACCAAACG CGTGCGTCTG 
GGACTGCAGG CCCAGCCGGT GCCTGATGAG CTGGTGGCCA AGCTTTTGGG GAACCAGGCC 
ACGTTCAGTC CTGTGGTGAC GGTGGAGCCT CGGCGCCGGA AGTTTCACCG TCCCATCGGC 
CTAAGAATCC CACTGCCACC CTCATGGAGA GAGAGCCCGC GGGACGCCGG AGAGGGAGAC 
ACCACCAGCC TGCGCCTGCT CTGCAGCGTC ATCGGTGGAA CAGCTCCTGC CCAGTGGGAA 
GATATAACTG GCACCACCAA ACTGGCGTAT TCCAATGACT GTGCCAGTTT CACCACCAAC 
GTCTCTGCCC GATTCTGGCT GGCGGACTGT CCTCGGACTG CGGAGGCCGT GTCCTTTGCC 
AACCTCCTGT ACCGCGAGCT GTCGGCCGTG CCGTACATGG CCAAGTTTGT AGTGTTTGCG 
AAGATGAATG AAGCTCGCGA GGGTCGACTC CGCTGCTACT GCATGACGGA TGACAAGATG 
GACAAAACCC TGGAGCAGCA CGAGAACTTC TCGGAAGTGG CTCGCAGCAG AGACATCGAG 
GTGATGGAGG GAATGCCGCT GTATTTGGAG TGTTCAGGAA ACTTGGTTCC TATAAGAAAA 
GCAGCCCAGC AGCCTCGCTG TTTCAGCTTC CAGGCCTTTA AAGACAACAG ACTGCCTGTG 
TCCGTCAAGG TGAGGGACAG CAGTAAAGAC CCCTCTGGGT TTTTGTCTTT CTTGCGCAAG 
TCAACCAAAT ATGAAGACAG CCAACACGTG CTGTGCAACC TCAACGTGAC CATGCCGCCG 
TGCATTAAGA TTATGGGCAG CGATGAGCGG AGGAGGACTC TGACTCCTCT GGCTCTCAGA 
GAAAGATACA GCGCTCTGAA TGAACCGGCA ATGGCATCAC TGAGCGCCAT GGAAAGGACG 
GAGTTAAAGA TGGCTTTAAT AGCTGAACAG CTAGGCCTGA GCTGGGCTGA GTTGGGACGG 
GAACTGCAGT TTAATGTAGA TGAAATAAAT AAGATCCGTG TGGAGAATCC CAACTCCCTT 
TTAGAGCAGA GCTCAACGCT GCTCAACCTG TGGGCCGCCC GAGAGGGGAA GAGAGCCAAA 
ATGGACAGCC TGTACGTGGC CCTCAAGAGC ATCGACCGAG TGGACATTGT GAACATGATG 
GAGGGCCAGG GAGGACCACC GGCGGGTCAG CAGGGGGGCT GGGAGCAGGA CGCTTCTAGG 
TCTAGGCATC ACGACCGAGA CCATCTCTCA CCTGGAATCA CAAATGGTTA CGGGCTGGTG 
GTGCAGGAGG AACTAGTGTC ACCGGCCTCC ATGCAGTACA GTCTGCCCTC GCCACTGGGC 
AATGAGCCCT ACTGGCAGGA GGTTTCCAGC ATGGAGTGCG CGCCCATCGC CACCACTGAA 
GAAGACACGC TCATGGAGAT GTCCGAGGTA CAGGTTTGGC CCTCAGGAAA TAGTCCGTCC 
ATTGTGGCTG TAGAGGACTC CTCATTGGAG TGCAGCAATG CGGACGACTC CGAGGCGCTG 
CAGAACCGGA GCCTGGGTCA CTCCAGGAGC GGGACGCCCG GTGGGAACGG ACTCACTCCG 
GGAGGTTTAA GTGGCTCCAT CGAGCTCCTG GAGGATCAGT CCATCGGTGC GGATTCAGAC 
TACAGTGCCG CCGGGATGCT GCGTCTGAGC GAAGGGGTCA ACATCAGTGC TCTGGACAGA 
GGTCAGAGGT CGGAGAGTTC AGAAGGGGCT GTAGGTCACC AAGTCACCAA CAACATTAAA 
GACGGTTCCA ATGGAGGTTT GGGAGAAGGA GGAGGTGACG GCACACAGTT AGAAGATAGC 
ATATCTCTCA TTTCAGGGCA ACAGCGTGTG TATGCTCGAA TGAGCGAGTC GCCTGGGCTG 
AGAAGACGGG CAGATCCTGG CGAAGACAGG TTAAGCGCAG GGTCGTTCCT GTCTTATCTG 
CAGGATCAGG GCTCTCCTGG TTGGCATTCG GCTGCAGAAG CGACTCAGAA CAGATTGGCG 
TCTTTGCAAT GCTCCCTTCC TAGGCAGGCA ATAGAGTCTA TGATGTCATC GGTGCGAGCT 
ACGGTGGACT CTGACTCCAA GCAGTCTTGT TTGGGCCAGG AGGCTCTGAT TGAGCCAGTG 
CGGGACATGG GGCACTCTGA GATCATCCAT GGCCATTATC AGGGGACTCA GCCGTTTGAG 
AAAGGACTCG GCTTCCCGCA CAGAACAACC AACATGCGCA CTTGGGAGGA CATGCGTCTG 
AGCGGACAGG GTGATGAGGC TGAGGATCTT CCAGGAGAGC AAGTGAGCGA GGAGCAATTC 
ACAGACGAGC ATGGCAACAT CGTCACCAAA AAGATTGTGA GGAAAGTGGT CCGGAGAGGA 
AAAGGGGAGG AAGGGGGTCA GGAGCTGATC ATTGAAGGTC TGCAGCAGGA TATCAGTGAG 
CCTGACTTTG ATGGAGAACA GTTCATGAGC TATGCCGTCC TGGGACGTGA CAGCAAGCCG 
GATGTTGTGG ATGTGAAGAG AGGAGGTGCT CAGATAGTCA AATGTGCGAG TCTGCGGAGA 
GTAAAGCAGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_021476109.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331784.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X11, mRNA.

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CloneID	ODa142601
Clone ID Related Accession (Same CDS sequence)	XM_021476109.1
Accession Version	XM_021476109.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6009bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin 1, erythrocytic a isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCTG ACGCTGCCAC CAGTTTCCTC CGCGCAGCTC GCTCAGGTAA TCTGGAAAAG 
GCTCTAGACC ACATCAAGAA CGGCATCGAC ATCAACACAG CCAATCAGAA TGGACTCAAC 
GGGCTGCATT TGGCCTCCAA GGAAGGCCAT GTTAAAATGG TGCTGGAGCT GCTTCACCAT 
GGTATTGTAC TTGAGACGAC AACGAAGAAA GGGAACACTG CTCTTCATAT AGCGGCGTTG 
GCTGGCCAGG AGCAGGTGGT CACTGAACTG GTCAACTATG GAGCAAACGT CAACGCTCAG 
TCTCAGAAGG GCTTCACGCC GCTCTACATG GCAGCACAAG AGAATCATCT GGAGGTTGTG 
AAGTTTCTGC TGGATAACGG AGCCAATCAG ACCATTCCAA CTGAGGATGG CTTTACCCCT 
CTTGCTGTGG CTCTTCAGCA GGGCCATGAG AATGTGGTGG CCCTGCTCAT CAACCACGGT 
ACTAAGGGCA AGGTCCGCCT TCCCGCACTG CACATCGCTG CCCGAAACGA CGACACCCGC 
ACTGCCGCTG TGCTCTTACA GAACGACCCC AATCCTGACG TCCTGAGCAA GACAGGCTTC 
ACGCCGCTCC ACATCGCAGC ACATTATGAA AACCTGAATG TTGCTCAGCT TCTTCTGAAC 
CGAGGAGCTG ATGTGAACTT CACACCTAAG AACGGGATCA CACCGCTGCA CATCGCATCC 
AGGAGAGGGA ACGTCATCAT GGTCAGACTT CTGCTGGACC GAGGAGCAAA GATCGATGCC 
AAGACAAAAG ATGAGTTGAC TCCTCTTCAC TGTGCTGCCA GAAATGGCCA TGTGAGAATC 
ATCGAGATCC TTCTGGACCA AGGAGCCCCA ATACAGGCCA AGACCAAAAA TGGCCTGTCG 
CCGATCCACA TGGCAGCCCA GGGCGATCAC TTGGACTGTA TCCGACAGCT CTTACAGTAC 
AACGCAGAGA TCGATGACAT CACTCTGGAC CACCTGACAC CTCTGCATGT GGCGGCTCAC 
TGCGGGCATC ACCGCGTGGC CAAGGTGCTC CTGGATAAAG GAGCCAAACC AAACACCCGC 
GCTCTGAATG GATTCACGCC GCTCCACATT GCCTGTAAGA AGAACCACAT GCGTGTAATG 
GACCTGCTAT TAAAGCATTC TGCATCCCTG GAAGCTGTAA CCGAGTCAGG CTTGACCCCG 
CTGCATGTGT CCTCCTTCAT GGGTCATCTG AATATAGTCA AGATACTGAT GCAAAAAGGA 
GCTTCGCCGA ACGCCTCAAA TGTGAAAGTG GAAACCCCTC TGCATATGGC ATCTCGAGCT 
GGACACTGTG AAGTTGCTGA ATTCCTATTG CAAAATGCAG CTCCAGTTGA TGCCAAAGCC 
AAGGATGACC AGACGCCGCT ACACTGTGCC TCCCGCATGG GCCACAACGA GATGGTGAAG 
TTGCTTCTGG AGCACAAGGC CAACTCAAAC TCCACCACCA CAGCAGGACA CACACCGCTG 
CACATCGCGG CCCGCGAGGG ACACACACAA ACTGCCAGCA TCCTCCTGGA CATGAACGCC 
CAGCTCACCA AAATGACCAA GAAAGGCTTC ACTCCTCTTC ATGTTGCAGC CAAATACGGA 
AAGGTGGACG TGGCAGTTCT GCTTCTGGAG AGGGGAGCAA ATCCAAACGC TGCTGGAAAA 
GTTGGACTCA CGCCGCTGCA TGTGGCCGTC CATCATAACA ATCTAGATGT GGTCAATCTT 
TTACTCAGCA AAGGAGGATC ACCACACAGC GCCGCTCGGA ATGGCTATAC TCCGCTCCAC 
ATTGCTTCCA AACAGAACCA GGCGGAGGTG GCGAGCAGTT TACTGCAGCA CGGGGCGTCT 
GCCAATGCTG AGTCTCTGCA AGGGGTCACA CCTCTACACC TCGCCTCTCA AGAGGGTCAA 
CCTGACATGG TGTTACTGCT CATCTCCAAG CAGGCCAATG TAAACCTTGG CAATAAGAGC 
GGTCTGACTC CTCTTCATCT TGTGGCTCAG GAAGGGCATG TTGGGATTGC TGATATACTA 
GTGAAACATG GAGCATCTGT TTATGCTGCC ACACGGATGG GTTACACTCC GCTTCACGTG 
GCTTGTCATT ATGGGAATAT CAAGATGGTG AAGTTTCTCT TACAGCAGCA GGCCAACGTA 
AACTGCAAGA CCAGGATGAG CTACACGCCG CTACATCAGG CTGCTCAGCA GGGTCATACG 
GACATCGTGA CGCTTCTGCT GAAACACGGA GCACAACCGA ATGAAGTTAC ATCTAACGGC 
ACCTCTGCTC TTGCCATCGC CAAGCGGCTT GGATACATTT CAGTCATCGA TGTGCTCAAA 
CTGGTCACTG AAGAGACGGT CACTATGACC ACCACTGAAA AACATCGCAT GAGTTTCCCT 
GAAACCGTTG ATGAGATCCT GGATGTGTCT GAAGATGAAG GAATTGCACA GCTAACATTA 
GGCGAGGAGC TGCTGGGAAA CGAGGGCGCC AGGTATTTGA TGAAGATGGA GGACATAAAG 
GACCATGATG AGGATTTCCT TTCCCCCAAG AAAGTTCTAG AGTACGAGGG AGAGTTGGAG 
AATCATTCTC CTGCTATCCC GAGGATCCCG TGTGTGTCTC CTGAGACTGT CATGCTGAAA 
GAGCATGAGA TGGAGCAGCA GCACACTCCA CTTCCATTGC CCAAAGAGTA CGACGAGGAT 
TCTCTGATTC CCAGCAGTCC TGCAACTGAG ACTTCAGACA ATGTCAGTCC GGTTGCCAGT 
CCCATCCACA CCGGTTTTCT GGTGAGCTTC ATGGTGGATG CTCGCGGCGG CTCGATGCGC 
GGCAGCAGAC ACAACGGCCT CCGCGTCATC ATTCCTCCTC GAACCTGCGC CGCTCCCACA 
CGCATCACCT GCCGCCTGGT GAAGCCCCAG AAGCTGCCCA CACCGCCGCC ATTAGTGGAG 
GGAGAGGGTC TCGCCAGCCG CATCATCTCC CTTGGACCCG CCAGCATGCA GTTTTTAGGG 
CCTGTGATTG TGGAGATTCC TCATTTTGCG GCTCTCGGTC GGGGAGATCG GGAACTCGTG 
GTCTTGAGGA GTGAGAACGG ATCGGTCTGG AAGGAACATC GTAACCGATA TGGAGATGAA 
GTGCTAGAGA CCATCCTCAA CGGCATGGAT GAAGATCTGG AGAGTCAGGA GGAGCTCGGG 
AAGAAACGCA TCAGACGGAT CATCTCCACC GACTTCCCGC TCTACTTTGC GGTGGTGTCT 
CGCATCCAGC AGGAGAACGA TCTGATTGGC CCAGAAGGAG GTTTTCTGTC CAGTAAACTG 
GTGCCGATGG TCCAGGCCAC CTTTCCAGAG ACAGCCGTCA CCAAACGCGT GCGTCTGGGA 
CTGCAGGCCC AGCCGGTGCC TGATGAGCTG GTGGCCAAGC TTTTGGGGAA CCAGGCCACG 
TTCAGTCCTG TGGTGACGGT GGAGCCTCGG CGCCGGAAGT TTCACCGTCC CATCGGCCTA 
AGAATCCCAC TGCCACCCTC ATGGAGAGAG AGCCCGCGGG ACGCCGGAGA GGGAGACACC 
ACCAGCCTGC GCCTGCTCTG CAGCGTCATC GGTGGAACAG CTCCTGCCCA GTGGGAAGAT 
ATAACTGGCA CCACCAAACT GGCGTATTCC AATGACTGTG CCAGTTTCAC CACCAACGTC 
TCTGCCCGAT TCTGGCTGGC GGACTGTCCT CGGACTGCGG AGGCCGTGTC CTTTGCCAAC 
CTCCTGTACC GCGAGCTGTC GGCCGTGCCG TACATGGCCA AGTTTGTAGT GTTTGCGAAG 
ATGAATGAAG CTCGCGAGGG TCGACTCCGC TGCTACTGCA TGACGGATGA CAAGATGGAC 
AAAACCCTGG AGCAGCACGA GAACTTCTCG GAAGTGGCTC GCAGCAGAGA CATCGAGGTG 
ATGGAGGGAA TGCCGCTGTA TTTGGAGTGT TCAGGAAACT TGGTTCCTAT AAGAAAAGCA 
GCCCAGCAGC CTCGCTGTTT CAGCTTCCAG GCCTTTAAAG ACAACAGACT GCCTGTGTCC 
GTCAAGGTGA GGGACAGCAG TAAAGACCCC TCTGGGTTTT TGTCTTTCTT GCGCAAGTCA 
ACCAAATATG AAGACAGCCA ACACGTGCTG TGCAACCTCA ACGTGACCAT GCCGCCGTGC 
ATTAAGATTA TGGGCAGCGA TGAGCGGAGG AGGACTCTGA CTCCTCTGGC TCTCAGAGAA 
AGATACAGCG CTCTGAATGA ACCGGCAATG GCATCACTGA GCGCCATGGA AAGGACGGAG 
TTAAAGATGG CTTTAATAGC TGAACAGCTA GGCCTGAGCT GGGCTGAGTT GGGACGGGAA 
CTGCAGTTTA ATGTAGATGA AATAAATAAG ATCCGTGTGG AGAATCCCAA CTCCCTTTTA 
GAGCAGAGCT CAACGCTGCT CAACCTGTGG GCCGCCCGAG AGGGGAAGAG AGCCAAAATG 
GACAGCCTGT ACGTGGCCCT CAAGAGCATC GACCGAGTGG ACATTGTGAA CATGATGGAG 
GGCCAGGGAG GACCACCGGC GGGTCAGCAG GGGGGCTGGG AGCAGGACGC TTCTAGGTCT 
AGGCATCACG ACCGAGACCA TCTCTCACCT GGAATCACAA ATGGTTACGG GCTGGTGGTG 
CAGGAGGAAC TAGTGTCACC GGCCTCCATG CAGTACAGTC TGCCCTCGCC ACTGGGCAAT 
GAGCCCTACT GGCAGGAGGT TTCCAGCATG GAGTGCGCGC CCATCGCCAC CACTGAAGAA 
GACACGCTCA TGGAGATGTC CGAGGTACAG GTTTGGCCCT CAGGAAATAG TCCGTCCATT 
GTGGCTGTAG AGGACTCCTC ATTGGAGTGC AGCAATGCGG ACGACTCCGA GGCGCTGCAG 
AACCGGAGCC TGGGTCACTC CAGGAGCGGG ACGCCCGGTG GGAACGGACT CACTCCGGGA 
GGTTTAAGTG GCTCCATCGA GCTCCTGGAG GATCAGTCCA TCGGTGCGGA TTCAGACTAC 
AGTGCCGCCG GGATGCTGCG TCTGAGCGAA GGGGTCAACA TCAGTGCTCT GGACAGAGGT 
CAGAGGTCGG AGAGTTCAGA AGGGGCTGTA GGTCACCAAG TCACCAACAA CATTAAAGAC 
GGTTCCAATG GAGGTTTGGG AGAAGGAGGA GGTGACGGCA CACAGTTAGA AGATAGCATA 
TCTCTCATTT CAGGGCAACA GCGTGTGTAT GCTCGAATGA GCGAGTCGCC TGGGCTGAGA 
AGACGGGCAG ATCCTGGCGA AGACAGGTTA AGCGCAGGGT CGTTCCTGTC TTATCTGCAG 
GATCAGGGCT CTCCTGGTTG GCATTCGGCT GCAGAAGCGA CTCAGAACAG ATTGGCGTCT 
TTGCAATGCT CCCTTCCTAG GCAGGCAATA GAGTCTATGA TGTCATCGGT GCGAGCTACG 
GTGGACTCTG ACTCCAAGCA GTCTTGTTTG GGCCAGGAGG CTCTGATTGA GCCAGTGCGG 
GACATGGGGC ACTCTGAGAT CATCCATGGC CATTATCAGG GGACTCAGCC GTTTGAGAAA 
GGACTCGGCT TCCCGCACAG AACAACCAAC ATGCGCACTT GGGAGGACAT GCGTCTGAGC 
GGACAGGGTG ATGAGGCTGA GGATCTTCCA GGAGAGCAAG TGAGCGAGGA GCAATTCACA 
GACGAGCATG GCAACATCGT CACCAAAAAG ATTGTGAGGA AAGTGGTCCG GAGAGGAAAA 
GGGGAGGAAG GGGGTCAGGA GCTGATCATT GAAGGTCTGC AGCAGGATAT CAGTGAGCCT 
GACTTTGATG GAGAACAGTT CATGAGCTAT GCCGTCCTGG GACGTGACAG CAAGCCGGAT 
GTTGTGGATG TGAAGAGAGG AGGTGCTCAG ATAGTCAAAT GTGCGAGTCT GCGGAGAGTA 
AAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331784.1
CDS	222..6230
Translation	MAQAAKHLRKNKDLEALAEQERKEKEEEKSKKRSRSREKKRKADAATSFLRAARSGNLEKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLINHGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGLTPLHVSSFMGHLNIVKILMQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGANPNAAGKVGLTPLHVAVHHNNLDVVNLLLSKGGSPHSAARNGYTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPLHLASQEGQPDMVLLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKHGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSNGTSALAIAKRLGYISVIDVLKLVTEETVTMTTTEKHRMSFPETVDEILDVSEDEGIAQLTLGEELLGNEGARYLMKMEDIKDHDEDFLSPKKVLEYEGELENHSPAIPRIPCVSPETVMLKEHEMEQQHTPLPLPKEYDEDSLIPSSPATETSDNVSPVASPIHTGFLVSFMVDARGGSMRGSRHNGLRVIIPPRTCAAPTRITCRLVKPQKLPTPPPLVEGEGLASRIISLGPASMQFLGPVIVEIPHFAALGRGDRELVVLRSENGSVWKEHRNRYGDEVLETILNGMDEDLESQEELGKKRIRRIISTDFPLYFAVVSRIQQENDLIGPEGGFLSSKLVPMVQATFPETAVTKRVRLGLQAQPVPDELVAKLLGNQATFSPVVTVEPRRRKFHRPIGLRIPLPPSWRESPRDAGEGDTTSLRLLCSVIGGTAPAQWEDITGTTKLAYSNDCASFTTNVSARFWLADCPRTAEAVSFANLLYRELSAVPYMAKFVVFAKMNEAREGRLRCYCMTDDKMDKTLEQHENFSEVARSRDIEVMEGMPLYLECSGNLVPIRKAAQQPRCFSFQAFKDNRLPVSVKVRDSSKDPSGFLSFLRKSTKYEDSQHVLCNLNVTMPPCIKIMGSDERRRTLTPLALRERYSALNEPAMASLSAMERTELKMALIAEQLGLSWAELGRELQFNVDEINKIRVENPNSLLEQSSTLLNLWAAREGKRAKMDSLYVALKSIDRVDIVNMMEGQGGPPAGQQGGWEQDASRSRHHDRDHLSPGITNGYGLVVQEELVSPASMQYSLPSPLGNEPYWQEVSSMECAPIATTEEDTLMEMSEVQVWPSGNSPSIVAVEDSSLECSNADDSEALQNRSLGHSRSGTPGGNGLTPGGLSGSIELLEDQSIGADSDYSAAGMLRLSEGVNISALDRGQRSESSEGAVGHQVTNNIKDGSNGGLGEGGGDGTQLEDSISLISGQQRVYARMSESPGLRRRADPGEDRLSAGSFLSYLQDQGSPGWHSAAEATQNRLASLQCSLPRQAIESMMSSVRATVDSDSKQSCLGQEALIEPVRDMGHSEIIHGHYQGTQPFEKGLGFPHRTTNMRTWEDMRLSGQGDEAEDLPGEQVSEEQFTDEHGNIVTKKIVRKVVRRGKGEEGGQELIIEGLQQDISEPDFDGEQFMSYAVLGRDSKPDVVDVKRGGAQIVKCASLRRVKQ

Target ORF information:

RefSeq Version	XM_021476109.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476109.1

ORF Insert Sequence:

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCTG ACGCTGCCAC CAGTTTCCTC CGCGCAGCTC GCTCAGGTAA TCTGGAAAAG 
GCTCTAGACC ACATCAAGAA CGGCATCGAC ATCAACACAG CCAATCAGAA TGGACTCAAC 
GGGCTGCATT TGGCCTCCAA GGAAGGCCAT GTTAAAATGG TGCTGGAGCT GCTTCACCAT 
GGTATTGTAC TTGAGACGAC AACGAAGAAA GGGAACACTG CTCTTCATAT AGCGGCGTTG 
GCTGGCCAGG AGCAGGTGGT CACTGAACTG GTCAACTATG GAGCAAACGT CAACGCTCAG 
TCTCAGAAGG GCTTCACGCC GCTCTACATG GCAGCACAAG AGAATCATCT GGAGGTTGTG 
AAGTTTCTGC TGGATAACGG AGCCAATCAG ACCATTCCAA CTGAGGATGG CTTTACCCCT 
CTTGCTGTGG CTCTTCAGCA GGGCCATGAG AATGTGGTGG CCCTGCTCAT CAACCACGGT 
ACTAAGGGCA AGGTCCGCCT TCCCGCACTG CACATCGCTG CCCGAAACGA CGACACCCGC 
ACTGCCGCTG TGCTCTTACA GAACGACCCC AATCCTGACG TCCTGAGCAA GACAGGCTTC 
ACGCCGCTCC ACATCGCAGC ACATTATGAA AACCTGAATG TTGCTCAGCT TCTTCTGAAC 
CGAGGAGCTG ATGTGAACTT CACACCTAAG AACGGGATCA CACCGCTGCA CATCGCATCC 
AGGAGAGGGA ACGTCATCAT GGTCAGACTT CTGCTGGACC GAGGAGCAAA GATCGATGCC 
AAGACAAAAG ATGAGTTGAC TCCTCTTCAC TGTGCTGCCA GAAATGGCCA TGTGAGAATC 
ATCGAGATCC TTCTGGACCA AGGAGCCCCA ATACAGGCCA AGACCAAAAA TGGCCTGTCG 
CCGATCCACA TGGCAGCCCA GGGCGATCAC TTGGACTGTA TCCGACAGCT CTTACAGTAC 
AACGCAGAGA TCGATGACAT CACTCTGGAC CACCTGACAC CTCTGCATGT GGCGGCTCAC 
TGCGGGCATC ACCGCGTGGC CAAGGTGCTC CTGGATAAAG GAGCCAAACC AAACACCCGC 
GCTCTGAATG GATTCACGCC GCTCCACATT GCCTGTAAGA AGAACCACAT GCGTGTAATG 
GACCTGCTAT TAAAGCATTC TGCATCCCTG GAAGCTGTAA CCGAGTCAGG CTTGACCCCG 
CTGCATGTGT CCTCCTTCAT GGGTCATCTG AATATAGTCA AGATACTGAT GCAAAAAGGA 
GCTTCGCCGA ACGCCTCAAA TGTGAAAGTG GAAACCCCTC TGCATATGGC ATCTCGAGCT 
GGACACTGTG AAGTTGCTGA ATTCCTATTG CAAAATGCAG CTCCAGTTGA TGCCAAAGCC 
AAGGATGACC AGACGCCGCT ACACTGTGCC TCCCGCATGG GCCACAACGA GATGGTGAAG 
TTGCTTCTGG AGCACAAGGC CAACTCAAAC TCCACCACCA CAGCAGGACA CACACCGCTG 
CACATCGCGG CCCGCGAGGG ACACACACAA ACTGCCAGCA TCCTCCTGGA CATGAACGCC 
CAGCTCACCA AAATGACCAA GAAAGGCTTC ACTCCTCTTC ATGTTGCAGC CAAATACGGA 
AAGGTGGACG TGGCAGTTCT GCTTCTGGAG AGGGGAGCAA ATCCAAACGC TGCTGGAAAA 
GTTGGACTCA CGCCGCTGCA TGTGGCCGTC CATCATAACA ATCTAGATGT GGTCAATCTT 
TTACTCAGCA AAGGAGGATC ACCACACAGC GCCGCTCGGA ATGGCTATAC TCCGCTCCAC 
ATTGCTTCCA AACAGAACCA GGCGGAGGTG GCGAGCAGTT TACTGCAGCA CGGGGCGTCT 
GCCAATGCTG AGTCTCTGCA AGGGGTCACA CCTCTACACC TCGCCTCTCA AGAGGGTCAA 
CCTGACATGG TGTTACTGCT CATCTCCAAG CAGGCCAATG TAAACCTTGG CAATAAGAGC 
GGTCTGACTC CTCTTCATCT TGTGGCTCAG GAAGGGCATG TTGGGATTGC TGATATACTA 
GTGAAACATG GAGCATCTGT TTATGCTGCC ACACGGATGG GTTACACTCC GCTTCACGTG 
GCTTGTCATT ATGGGAATAT CAAGATGGTG AAGTTTCTCT TACAGCAGCA GGCCAACGTA 
AACTGCAAGA CCAGGATGAG CTACACGCCG CTACATCAGG CTGCTCAGCA GGGTCATACG 
GACATCGTGA CGCTTCTGCT GAAACACGGA GCACAACCGA ATGAAGTTAC ATCTAACGGC 
ACCTCTGCTC TTGCCATCGC CAAGCGGCTT GGATACATTT CAGTCATCGA TGTGCTCAAA 
CTGGTCACTG AAGAGACGGT CACTATGACC ACCACTGAAA AACATCGCAT GAGTTTCCCT 
GAAACCGTTG ATGAGATCCT GGATGTGTCT GAAGATGAAG GAATTGCACA GCTAACATTA 
GGCGAGGAGC TGCTGGGAAA CGAGGGCGCC AGGTATTTGA TGAAGATGGA GGACATAAAG 
GACCATGATG AGGATTTCCT TTCCCCCAAG AAAGTTCTAG AGTACGAGGG AGAGTTGGAG 
AATCATTCTC CTGCTATCCC GAGGATCCCG TGTGTGTCTC CTGAGACTGT CATGCTGAAA 
GAGCATGAGA TGGAGCAGCA GCACACTCCA CTTCCATTGC CCAAAGAGTA CGACGAGGAT 
TCTCTGATTC CCAGCAGTCC TGCAACTGAG ACTTCAGACA ATGTCAGTCC GGTTGCCAGT 
CCCATCCACA CCGGTTTTCT GGTGAGCTTC ATGGTGGATG CTCGCGGCGG CTCGATGCGC 
GGCAGCAGAC ACAACGGCCT CCGCGTCATC ATTCCTCCTC GAACCTGCGC CGCTCCCACA 
CGCATCACCT GCCGCCTGGT GAAGCCCCAG AAGCTGCCCA CACCGCCGCC ATTAGTGGAG 
GGAGAGGGTC TCGCCAGCCG CATCATCTCC CTTGGACCCG CCAGCATGCA GTTTTTAGGG 
CCTGTGATTG TGGAGATTCC TCATTTTGCG GCTCTCGGTC GGGGAGATCG GGAACTCGTG 
GTCTTGAGGA GTGAGAACGG ATCGGTCTGG AAGGAACATC GTAACCGATA TGGAGATGAA 
GTGCTAGAGA CCATCCTCAA CGGCATGGAT GAAGATCTGG AGAGTCAGGA GGAGCTCGGG 
AAGAAACGCA TCAGACGGAT CATCTCCACC GACTTCCCGC TCTACTTTGC GGTGGTGTCT 
CGCATCCAGC AGGAGAACGA TCTGATTGGC CCAGAAGGAG GTTTTCTGTC CAGTAAACTG 
GTGCCGATGG TCCAGGCCAC CTTTCCAGAG ACAGCCGTCA CCAAACGCGT GCGTCTGGGA 
CTGCAGGCCC AGCCGGTGCC TGATGAGCTG GTGGCCAAGC TTTTGGGGAA CCAGGCCACG 
TTCAGTCCTG TGGTGACGGT GGAGCCTCGG CGCCGGAAGT TTCACCGTCC CATCGGCCTA 
AGAATCCCAC TGCCACCCTC ATGGAGAGAG AGCCCGCGGG ACGCCGGAGA GGGAGACACC 
ACCAGCCTGC GCCTGCTCTG CAGCGTCATC GGTGGAACAG CTCCTGCCCA GTGGGAAGAT 
ATAACTGGCA CCACCAAACT GGCGTATTCC AATGACTGTG CCAGTTTCAC CACCAACGTC 
TCTGCCCGAT TCTGGCTGGC GGACTGTCCT CGGACTGCGG AGGCCGTGTC CTTTGCCAAC 
CTCCTGTACC GCGAGCTGTC GGCCGTGCCG TACATGGCCA AGTTTGTAGT GTTTGCGAAG 
ATGAATGAAG CTCGCGAGGG TCGACTCCGC TGCTACTGCA TGACGGATGA CAAGATGGAC 
AAAACCCTGG AGCAGCACGA GAACTTCTCG GAAGTGGCTC GCAGCAGAGA CATCGAGGTG 
ATGGAGGGAA TGCCGCTGTA TTTGGAGTGT TCAGGAAACT TGGTTCCTAT AAGAAAAGCA 
GCCCAGCAGC CTCGCTGTTT CAGCTTCCAG GCCTTTAAAG ACAACAGACT GCCTGTGTCC 
GTCAAGGTGA GGGACAGCAG TAAAGACCCC TCTGGGTTTT TGTCTTTCTT GCGCAAGTCA 
ACCAAATATG AAGACAGCCA ACACGTGCTG TGCAACCTCA ACGTGACCAT GCCGCCGTGC 
ATTAAGATTA TGGGCAGCGA TGAGCGGAGG AGGACTCTGA CTCCTCTGGC TCTCAGAGAA 
AGATACAGCG CTCTGAATGA ACCGGCAATG GCATCACTGA GCGCCATGGA AAGGACGGAG 
TTAAAGATGG CTTTAATAGC TGAACAGCTA GGCCTGAGCT GGGCTGAGTT GGGACGGGAA 
CTGCAGTTTA ATGTAGATGA AATAAATAAG ATCCGTGTGG AGAATCCCAA CTCCCTTTTA 
GAGCAGAGCT CAACGCTGCT CAACCTGTGG GCCGCCCGAG AGGGGAAGAG AGCCAAAATG 
GACAGCCTGT ACGTGGCCCT CAAGAGCATC GACCGAGTGG ACATTGTGAA CATGATGGAG 
GGCCAGGGAG GACCACCGGC GGGTCAGCAG GGGGGCTGGG AGCAGGACGC TTCTAGGTCT 
AGGCATCACG ACCGAGACCA TCTCTCACCT GGAATCACAA ATGGTTACGG GCTGGTGGTG 
CAGGAGGAAC TAGTGTCACC GGCCTCCATG CAGTACAGTC TGCCCTCGCC ACTGGGCAAT 
GAGCCCTACT GGCAGGAGGT TTCCAGCATG GAGTGCGCGC CCATCGCCAC CACTGAAGAA 
GACACGCTCA TGGAGATGTC CGAGGTACAG GTTTGGCCCT CAGGAAATAG TCCGTCCATT 
GTGGCTGTAG AGGACTCCTC ATTGGAGTGC AGCAATGCGG ACGACTCCGA GGCGCTGCAG 
AACCGGAGCC TGGGTCACTC CAGGAGCGGG ACGCCCGGTG GGAACGGACT CACTCCGGGA 
GGTTTAAGTG GCTCCATCGA GCTCCTGGAG GATCAGTCCA TCGGTGCGGA TTCAGACTAC 
AGTGCCGCCG GGATGCTGCG TCTGAGCGAA GGGGTCAACA TCAGTGCTCT GGACAGAGGT 
CAGAGGTCGG AGAGTTCAGA AGGGGCTGTA GGTCACCAAG TCACCAACAA CATTAAAGAC 
GGTTCCAATG GAGGTTTGGG AGAAGGAGGA GGTGACGGCA CACAGTTAGA AGATAGCATA 
TCTCTCATTT CAGGGCAACA GCGTGTGTAT GCTCGAATGA GCGAGTCGCC TGGGCTGAGA 
AGACGGGCAG ATCCTGGCGA AGACAGGTTA AGCGCAGGGT CGTTCCTGTC TTATCTGCAG 
GATCAGGGCT CTCCTGGTTG GCATTCGGCT GCAGAAGCGA CTCAGAACAG ATTGGCGTCT 
TTGCAATGCT CCCTTCCTAG GCAGGCAATA GAGTCTATGA TGTCATCGGT GCGAGCTACG 
GTGGACTCTG ACTCCAAGCA GTCTTGTTTG GGCCAGGAGG CTCTGATTGA GCCAGTGCGG 
GACATGGGGC ACTCTGAGAT CATCCATGGC CATTATCAGG GGACTCAGCC GTTTGAGAAA 
GGACTCGGCT TCCCGCACAG AACAACCAAC ATGCGCACTT GGGAGGACAT GCGTCTGAGC 
GGACAGGGTG ATGAGGCTGA GGATCTTCCA GGAGAGCAAG TGAGCGAGGA GCAATTCACA 
GACGAGCATG GCAACATCGT CACCAAAAAG ATTGTGAGGA AAGTGGTCCG GAGAGGAAAA 
GGGGAGGAAG GGGGTCAGGA GCTGATCATT GAAGGTCTGC AGCAGGATAT CAGTGAGCCT 
GACTTTGATG GAGAACAGTT CATGAGCTAT GCCGTCCTGG GACGTGACAG CAAGCCGGAT 
GTTGTGGATG TGAAGAGAGG AGGTGCTCAG ATAGTCAAAT GTGCGAGTCT GCGGAGAGTA 
AAGCAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ank1a ( XM_021476122.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331797.1 Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X27, mRNA.

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CloneID	ODa142613
Clone ID Related Accession (Same CDS sequence)	XM_021476122.1
Accession Version	XM_021476122.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6006bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ankyrin 1, erythrocytic a isoform X26
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCTG ACGCTGCCAC CAGTTTCCTC CGCGCAGCTC GCTCAGGTAA TCTGGAAAAG 
GCTCTAGACC ACATCAAGAA CGGCATCGAC ATCAACACAG CCAATCAGAA TGGACTCAAC 
GGGCTGCATT TGGCCTCCAA GGAAGGCCAT GTTAAAATGG TGCTGGAGCT GCTTCACCAT 
GGTATTGTAC TTGAGACGAC AACGAAGAAA GGGAACACTG CTCTTCATAT AGCGGCGTTG 
GCTGGCCAGG AGCAGGTGGT CACTGAACTG GTCAACTATG GAGCAAACGT CAACGCTCAG 
TCTCAGAAGG GCTTCACGCC GCTCTACATG GCAGCACAAG AGAATCATCT GGAGGTTGTG 
AAGTTTCTGC TGGATAACGG AGCCAATCAG ACCATTCCAA CTGAGGATGG CTTTACCCCT 
CTTGCTGTGG CTCTTCAGCA GGGCCATGAG AATGTGGTGG CCCTGCTCAT CAACCACGGT 
ACTAAGGGCA AGGTCCGCCT TCCCGCACTG CACATCGCTG CCCGAAACGA CGACACCCGC 
ACTGCCGCTG TGCTCTTACA GAACGACCCC AATCCTGACG TCCTGAGCAA GACAGGCTTC 
ACGCCGCTCC ACATCGCAGC ACATTATGAA AACCTGAATG TTGCTCAGCT TCTTCTGAAC 
CGAGGAGCTG ATGTGAACTT CACACCTAAG AACGGGATCA CACCGCTGCA CATCGCATCC 
AGGAGAGGGA ACGTCATCAT GGTCAGACTT CTGCTGGACC GAGGAGCAAA GATCGATGCC 
AAGACAAAAG ATGAGTTGAC TCCTCTTCAC TGTGCTGCCA GAAATGGCCA TGTGAGAATC 
ATCGAGATCC TTCTGGACCA AGGAGCCCCA ATACAGGCCA AGACCAAAAA TGGCCTGTCG 
CCGATCCACA TGGCAGCCCA GGGCGATCAC TTGGACTGTA TCCGACAGCT CTTACAGTAC 
AACGCAGAGA TCGATGACAT CACTCTGGAC CACCTGACAC CTCTGCATGT GGCGGCTCAC 
TGCGGGCATC ACCGCGTGGC CAAGGTGCTC CTGGATAAAG GAGCCAAACC AAACACCCGC 
GCTCTGAATG GATTCACGCC GCTCCACATT GCCTGTAAGA AGAACCACAT GCGTGTAATG 
GACCTGCTAT TAAAGCATTC TGCATCCCTG GAAGCTGTAA CCGAGTCAGG CTTGACCCCG 
CTGCATGTGT CCTCCTTCAT GGGTCATCTG AATATAGTCA AGATACTGAT GCAAAAAGGA 
GCTTCGCCGA ACGCCTCAAA TGTGAAAGTG GAAACCCCTC TGCATATGGC ATCTCGAGCT 
GGACACTGTG AAGTTGCTGA ATTCCTATTG CAAAATGCAG CTCCAGTTGA TGCCAAAGCC 
AAGGATGACC AGACGCCGCT ACACTGTGCC TCCCGCATGG GCCACAACGA GATGGTGAAG 
TTGCTTCTGG AGCACAAGGC CAACTCAAAC TCCACCACCA CAGCAGGACA CACACCGCTG 
CACATCGCGG CCCGCGAGGG ACACACACAA ACTGCCAGCA TCCTCCTGGA CATGAACGCC 
CAGCTCACCA AAATGACCAA GAAAGGCTTC ACTCCTCTTC ATGTTGCAGC CAAATACGGA 
AAGGTGGACG TGGCAGTTCT GCTTCTGGAG AGGGGAGCAA ATCCAAACGC TGCTGGAAAA 
GTTGGACTCA CGCCGCTGCA TGTGGCCGTC CATCATAACA ATCTAGATGT GGTCAATCTT 
TTACTCAGCA AAGGAGGATC ACCACACAGC GCCGCTCGGA ATGGCTATAC TCCGCTCCAC 
ATTGCTTCCA AACAGAACCA GGCGGAGGTG GCGAGCAGTT TACTGCAGCA CGGGGCGTCT 
GCCAATGCTG AGTCTCTGCA AGGGGTCACA CCTCTACACC TCGCCTCTCA AGAGGGTCAA 
CCTGACATGG TGTTACTGCT CATCTCCAAG CAGGCCAATG TAAACCTTGG CAATAAGAGC 
GGTCTGACTC CTCTTCATCT TGTGGCTCAG GAAGGGCATG TTGGGATTGC TGATATACTA 
GTGAAACATG GAGCATCTGT TTATGCTGCC ACACGGATGG GTTACACTCC GCTTCACGTG 
GCTTGTCATT ATGGGAATAT CAAGATGGTG AAGTTTCTCT TACAGCAGCA GGCCAACGTA 
AACTGCAAGA CCAGGATGAG CTACACGCCG CTACATCAGG CTGCTCAGCA GGGTCATACG 
GACATCGTGA CGCTTCTGCT GAAACACGGA GCACAACCGA ATGAAGTTAC ATCTAACGGC 
ACCTCTGCTC TTGCCATCGC CAAGCGGCTT GGATACATTT CAGTCATCGA TGTGCTCAAA 
CTGGTCACTG AAGAGACGGT CACTATGACC ACCACTGAAA AACATCGCAT GAGTTTCCCT 
GAAACCGTTG ATGAGATCCT GGATGTGTCT GAAGATGAAG GAATTGCACA GCTAACATTA 
GGCGAGGAGC TGCTGGGAAA CGAGGGCGCC AGGTATTTGA TGAAGATGGA GGACATAAAG 
GACCATGATG AGGATTTCCT TTCCCCCAAG AAAGTTCTAG AGTACGAGGG AGAGTTGGAG 
AATCATTCTC CTGCTATCCC GAGGATCCCG TGTGTGTCTC CTGAGACTGT CATGCTGAAA 
GAGCATGAGA TGGAGCAGCA CACTCCACTT CCATTGCCCA AAGAGTACGA CGAGGATTCT 
CTGATTCCCA GCAGTCCTGC AACTGAGACT TCAGACAATG TCAGTCCGGT TGCCAGTCCC 
ATCCACACCG GTTTTCTGGT GAGCTTCATG GTGGATGCTC GCGGCGGCTC GATGCGCGGC 
AGCAGACACA ACGGCCTCCG CGTCATCATT CCTCCTCGAA CCTGCGCCGC TCCCACACGC 
ATCACCTGCC GCCTGGTGAA GCCCCAGAAG CTGCCCACAC CGCCGCCATT AGTGGAGGGA 
GAGGGTCTCG CCAGCCGCAT CATCTCCCTT GGACCCGCCA GCATGCAGTT TTTAGGGCCT 
GTGATTGTGG AGATTCCTCA TTTTGCGGCT CTCGGTCGGG GAGATCGGGA ACTCGTGGTC 
TTGAGGAGTG AGAACGGATC GGTCTGGAAG GAACATCGTA ACCGATATGG AGATGAAGTG 
CTAGAGACCA TCCTCAACGG CATGGATGAA GATCTGGAGA GTCAGGAGGA GCTCGGGAAG 
AAACGCATCA GACGGATCAT CTCCACCGAC TTCCCGCTCT ACTTTGCGGT GGTGTCTCGC 
ATCCAGCAGG AGAACGATCT GATTGGCCCA GAAGGAGGTT TTCTGTCCAG TAAACTGGTG 
CCGATGGTCC AGGCCACCTT TCCAGAGACA GCCGTCACCA AACGCGTGCG TCTGGGACTG 
CAGGCCCAGC CGGTGCCTGA TGAGCTGGTG GCCAAGCTTT TGGGGAACCA GGCCACGTTC 
AGTCCTGTGG TGACGGTGGA GCCTCGGCGC CGGAAGTTTC ACCGTCCCAT CGGCCTAAGA 
ATCCCACTGC CACCCTCATG GAGAGAGAGC CCGCGGGACG CCGGAGAGGG AGACACCACC 
AGCCTGCGCC TGCTCTGCAG CGTCATCGGT GGAACAGCTC CTGCCCAGTG GGAAGATATA 
ACTGGCACCA CCAAACTGGC GTATTCCAAT GACTGTGCCA GTTTCACCAC CAACGTCTCT 
GCCCGATTCT GGCTGGCGGA CTGTCCTCGG ACTGCGGAGG CCGTGTCCTT TGCCAACCTC 
CTGTACCGCG AGCTGTCGGC CGTGCCGTAC ATGGCCAAGT TTGTAGTGTT TGCGAAGATG 
AATGAAGCTC GCGAGGGTCG ACTCCGCTGC TACTGCATGA CGGATGACAA GATGGACAAA 
ACCCTGGAGC AGCACGAGAA CTTCTCGGAA GTGGCTCGCA GCAGAGACAT CGAGGTGATG 
GAGGGAATGC CGCTGTATTT GGAGTGTTCA GGAAACTTGG TTCCTATAAG AAAAGCAGCC 
CAGCAGCCTC GCTGTTTCAG CTTCCAGGCC TTTAAAGACA ACAGACTGCC TGTGTCCGTC 
AAGGTGAGGG ACAGCAGTAA AGACCCCTCT GGGTTTTTGT CTTTCTTGCG CAAGTCAACC 
AAATATGAAG ACAGCCAACA CGTGCTGTGC AACCTCAACG TGACCATGCC GCCGTGCATT 
AAGATTATGG GCAGCGATGA GCGGAGGAGG ACTCTGACTC CTCTGGCTCT CAGAGAAAGA 
TACAGCGCTC TGAATGAACC GGCAATGGCA TCACTGAGCG CCATGGAAAG GACGGAGTTA 
AAGATGGCTT TAATAGCTGA ACAGCTAGGC CTGAGCTGGG CTGAGTTGGG ACGGGAACTG 
CAGTTTAATG TAGATGAAAT AAATAAGATC CGTGTGGAGA ATCCCAACTC CCTTTTAGAG 
CAGAGCTCAA CGCTGCTCAA CCTGTGGGCC GCCCGAGAGG GGAAGAGAGC CAAAATGGAC 
AGCCTGTACG TGGCCCTCAA GAGCATCGAC CGAGTGGACA TTGTGAACAT GATGGAGGGC 
CAGGGAGGAC CACCGGCGGG TCAGCAGGGG GGCTGGGAGC AGGACGCTTC TAGGTCTAGG 
CATCACGACC GAGACCATCT CTCACCTGGA ATCACAAATG GTTACGGGCT GGTGGTGCAG 
GAGGAACTAG TGTCACCGGC CTCCATGCAG TACAGTCTGC CCTCGCCACT GGGCAATGAG 
CCCTACTGGC AGGAGGTTTC CAGCATGGAG TGCGCGCCCA TCGCCACCAC TGAAGAAGAC 
ACGCTCATGG AGATGTCCGA GGTACAGGTT TGGCCCTCAG GAAATAGTCC GTCCATTGTG 
GCTGTAGAGG ACTCCTCATT GGAGTGCAGC AATGCGGACG ACTCCGAGGC GCTGCAGAAC 
CGGAGCCTGG GTCACTCCAG GAGCGGGACG CCCGGTGGGA ACGGACTCAC TCCGGGAGGT 
TTAAGTGGCT CCATCGAGCT CCTGGAGGAT CAGTCCATCG GTGCGGATTC AGACTACAGT 
GCCGCCGGGA TGCTGCGTCT GAGCGAAGGG GTCAACATCA GTGCTCTGGA CAGAGGTCAG 
AGGTCGGAGA GTTCAGAAGG GGCTGTAGGT CACCAAGTCA CCAACAACAT TAAAGACGGT 
TCCAATGGAG GTTTGGGAGA AGGAGGAGGT GACGGCACAC AGTTAGAAGA TAGCATATCT 
CTCATTTCAG GGCAACAGCG TGTGTATGCT CGAATGAGCG AGTCGCCTGG GCTGAGAAGA 
CGGGCAGATC CTGGCGAAGA CAGGTTAAGC GCAGGGTCGT TCCTGTCTTA TCTGCAGGAT 
CAGGGCTCTC CTGGTTGGCA TTCGGCTGCA GAAGCGACTC AGAACAGATT GGCGTCTTTG 
CAATGCTCCC TTCCTAGGCA GGCAATAGAG TCTATGATGT CATCGGTGCG AGCTACGGTG 
GACTCTGACT CCAAGCAGTC TTGTTTGGGC CAGGAGGCTC TGATTGAGCC AGTGCGGGAC 
ATGGGGCACT CTGAGATCAT CCATGGCCAT TATCAGGGGA CTCAGCCGTT TGAGAAAGGA 
CTCGGCTTCC CGCACAGAAC AACCAACATG CGCACTTGGG AGGACATGCG TCTGAGCGGA 
CAGGGTGATG AGGCTGAGGA TCTTCCAGGA GAGCAAGTGA GCGAGGAGCA ATTCACAGAC 
GAGCATGGCA ACATCGTCAC CAAAAAGATT GTGAGGAAAG TGGTCCGGAG AGGAAAAGGG 
GAGGAAGGGG GTCAGGAGCT GATCATTGAA GGTCTGCAGC AGGATATCAG TGAGCCTGAC 
TTTGATGGAG AACAGTTCAT GAGCTATGCC GTCCTGGGAC GTGACAGCAA GCCGGATGTT 
GTGGATGTGA AGAGAGGAGG TGCTCAGATA GTCAAATGTG CGAGTCTGCG GAGAGTAAAG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331797.1
CDS	222..6227
Translation	MAQAAKHLRKNKDLEALAEQERKEKEEEKSKKRSRSREKKRKADAATSFLRAARSGNLEKALDHIKNGIDINTANQNGLNGLHLASKEGHVKMVLELLHHGIVLETTTKKGNTALHIAALAGQEQVVTELVNYGANVNAQSQKGFTPLYMAAQENHLEVVKFLLDNGANQTIPTEDGFTPLAVALQQGHENVVALLINHGTKGKVRLPALHIAARNDDTRTAAVLLQNDPNPDVLSKTGFTPLHIAAHYENLNVAQLLLNRGADVNFTPKNGITPLHIASRRGNVIMVRLLLDRGAKIDAKTKDELTPLHCAARNGHVRIIEILLDQGAPIQAKTKNGLSPIHMAAQGDHLDCIRQLLQYNAEIDDITLDHLTPLHVAAHCGHHRVAKVLLDKGAKPNTRALNGFTPLHIACKKNHMRVMDLLLKHSASLEAVTESGLTPLHVSSFMGHLNIVKILMQKGASPNASNVKVETPLHMASRAGHCEVAEFLLQNAAPVDAKAKDDQTPLHCASRMGHNEMVKLLLEHKANSNSTTTAGHTPLHIAAREGHTQTASILLDMNAQLTKMTKKGFTPLHVAAKYGKVDVAVLLLERGANPNAAGKVGLTPLHVAVHHNNLDVVNLLLSKGGSPHSAARNGYTPLHIASKQNQAEVASSLLQHGASANAESLQGVTPLHLASQEGQPDMVLLLISKQANVNLGNKSGLTPLHLVAQEGHVGIADILVKHGASVYAATRMGYTPLHVACHYGNIKMVKFLLQQQANVNCKTRMSYTPLHQAAQQGHTDIVTLLLKHGAQPNEVTSNGTSALAIAKRLGYISVIDVLKLVTEETVTMTTTEKHRMSFPETVDEILDVSEDEGIAQLTLGEELLGNEGARYLMKMEDIKDHDEDFLSPKKVLEYEGELENHSPAIPRIPCVSPETVMLKEHEMEQHTPLPLPKEYDEDSLIPSSPATETSDNVSPVASPIHTGFLVSFMVDARGGSMRGSRHNGLRVIIPPRTCAAPTRITCRLVKPQKLPTPPPLVEGEGLASRIISLGPASMQFLGPVIVEIPHFAALGRGDRELVVLRSENGSVWKEHRNRYGDEVLETILNGMDEDLESQEELGKKRIRRIISTDFPLYFAVVSRIQQENDLIGPEGGFLSSKLVPMVQATFPETAVTKRVRLGLQAQPVPDELVAKLLGNQATFSPVVTVEPRRRKFHRPIGLRIPLPPSWRESPRDAGEGDTTSLRLLCSVIGGTAPAQWEDITGTTKLAYSNDCASFTTNVSARFWLADCPRTAEAVSFANLLYRELSAVPYMAKFVVFAKMNEAREGRLRCYCMTDDKMDKTLEQHENFSEVARSRDIEVMEGMPLYLECSGNLVPIRKAAQQPRCFSFQAFKDNRLPVSVKVRDSSKDPSGFLSFLRKSTKYEDSQHVLCNLNVTMPPCIKIMGSDERRRTLTPLALRERYSALNEPAMASLSAMERTELKMALIAEQLGLSWAELGRELQFNVDEINKIRVENPNSLLEQSSTLLNLWAAREGKRAKMDSLYVALKSIDRVDIVNMMEGQGGPPAGQQGGWEQDASRSRHHDRDHLSPGITNGYGLVVQEELVSPASMQYSLPSPLGNEPYWQEVSSMECAPIATTEEDTLMEMSEVQVWPSGNSPSIVAVEDSSLECSNADDSEALQNRSLGHSRSGTPGGNGLTPGGLSGSIELLEDQSIGADSDYSAAGMLRLSEGVNISALDRGQRSESSEGAVGHQVTNNIKDGSNGGLGEGGGDGTQLEDSISLISGQQRVYARMSESPGLRRRADPGEDRLSAGSFLSYLQDQGSPGWHSAAEATQNRLASLQCSLPRQAIESMMSSVRATVDSDSKQSCLGQEALIEPVRDMGHSEIIHGHYQGTQPFEKGLGFPHRTTNMRTWEDMRLSGQGDEAEDLPGEQVSEEQFTDEHGNIVTKKIVRKVVRRGKGEEGGQELIIEGLQQDISEPDFDGEQFMSYAVLGRDSKPDVVDVKRGGAQIVKCASLRRVKQ

Target ORF information:

RefSeq Version	XM_021476122.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ankyrin 1, erythrocytic a (ank1a), transcript variant X27, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476122.1

ORF Insert Sequence:

ATGGCACAAG CCGCCAAACA TCTGCGCAAA AACAAGGATT TAGAGGCTCT CGCCGAGCAG 
GAGAGGAAGG AGAAGGAGGA GGAGAAGTCC AAGAAGAGGA GCCGATCGCG GGAGAAAAAG 
CGCAAGGCTG ACGCTGCCAC CAGTTTCCTC CGCGCAGCTC GCTCAGGTAA TCTGGAAAAG 
GCTCTAGACC ACATCAAGAA CGGCATCGAC ATCAACACAG CCAATCAGAA TGGACTCAAC 
GGGCTGCATT TGGCCTCCAA GGAAGGCCAT GTTAAAATGG TGCTGGAGCT GCTTCACCAT 
GGTATTGTAC TTGAGACGAC AACGAAGAAA GGGAACACTG CTCTTCATAT AGCGGCGTTG 
GCTGGCCAGG AGCAGGTGGT CACTGAACTG GTCAACTATG GAGCAAACGT CAACGCTCAG 
TCTCAGAAGG GCTTCACGCC GCTCTACATG GCAGCACAAG AGAATCATCT GGAGGTTGTG 
AAGTTTCTGC TGGATAACGG AGCCAATCAG ACCATTCCAA CTGAGGATGG CTTTACCCCT 
CTTGCTGTGG CTCTTCAGCA GGGCCATGAG AATGTGGTGG CCCTGCTCAT CAACCACGGT 
ACTAAGGGCA AGGTCCGCCT TCCCGCACTG CACATCGCTG CCCGAAACGA CGACACCCGC 
ACTGCCGCTG TGCTCTTACA GAACGACCCC AATCCTGACG TCCTGAGCAA GACAGGCTTC 
ACGCCGCTCC ACATCGCAGC ACATTATGAA AACCTGAATG TTGCTCAGCT TCTTCTGAAC 
CGAGGAGCTG ATGTGAACTT CACACCTAAG AACGGGATCA CACCGCTGCA CATCGCATCC 
AGGAGAGGGA ACGTCATCAT GGTCAGACTT CTGCTGGACC GAGGAGCAAA GATCGATGCC 
AAGACAAAAG ATGAGTTGAC TCCTCTTCAC TGTGCTGCCA GAAATGGCCA TGTGAGAATC 
ATCGAGATCC TTCTGGACCA AGGAGCCCCA ATACAGGCCA AGACCAAAAA TGGCCTGTCG 
CCGATCCACA TGGCAGCCCA GGGCGATCAC TTGGACTGTA TCCGACAGCT CTTACAGTAC 
AACGCAGAGA TCGATGACAT CACTCTGGAC CACCTGACAC CTCTGCATGT GGCGGCTCAC 
TGCGGGCATC ACCGCGTGGC CAAGGTGCTC CTGGATAAAG GAGCCAAACC AAACACCCGC 
GCTCTGAATG GATTCACGCC GCTCCACATT GCCTGTAAGA AGAACCACAT GCGTGTAATG 
GACCTGCTAT TAAAGCATTC TGCATCCCTG GAAGCTGTAA CCGAGTCAGG CTTGACCCCG 
CTGCATGTGT CCTCCTTCAT GGGTCATCTG AATATAGTCA AGATACTGAT GCAAAAAGGA 
GCTTCGCCGA ACGCCTCAAA TGTGAAAGTG GAAACCCCTC TGCATATGGC ATCTCGAGCT 
GGACACTGTG AAGTTGCTGA ATTCCTATTG CAAAATGCAG CTCCAGTTGA TGCCAAAGCC 
AAGGATGACC AGACGCCGCT ACACTGTGCC TCCCGCATGG GCCACAACGA GATGGTGAAG 
TTGCTTCTGG AGCACAAGGC CAACTCAAAC TCCACCACCA CAGCAGGACA CACACCGCTG 
CACATCGCGG CCCGCGAGGG ACACACACAA ACTGCCAGCA TCCTCCTGGA CATGAACGCC 
CAGCTCACCA AAATGACCAA GAAAGGCTTC ACTCCTCTTC ATGTTGCAGC CAAATACGGA 
AAGGTGGACG TGGCAGTTCT GCTTCTGGAG AGGGGAGCAA ATCCAAACGC TGCTGGAAAA 
GTTGGACTCA CGCCGCTGCA TGTGGCCGTC CATCATAACA ATCTAGATGT GGTCAATCTT 
TTACTCAGCA AAGGAGGATC ACCACACAGC GCCGCTCGGA ATGGCTATAC TCCGCTCCAC 
ATTGCTTCCA AACAGAACCA GGCGGAGGTG GCGAGCAGTT TACTGCAGCA CGGGGCGTCT 
GCCAATGCTG AGTCTCTGCA AGGGGTCACA CCTCTACACC TCGCCTCTCA AGAGGGTCAA 
CCTGACATGG TGTTACTGCT CATCTCCAAG CAGGCCAATG TAAACCTTGG CAATAAGAGC 
GGTCTGACTC CTCTTCATCT TGTGGCTCAG GAAGGGCATG TTGGGATTGC TGATATACTA 
GTGAAACATG GAGCATCTGT TTATGCTGCC ACACGGATGG GTTACACTCC GCTTCACGTG 
GCTTGTCATT ATGGGAATAT CAAGATGGTG AAGTTTCTCT TACAGCAGCA GGCCAACGTA 
AACTGCAAGA CCAGGATGAG CTACACGCCG CTACATCAGG CTGCTCAGCA GGGTCATACG 
GACATCGTGA CGCTTCTGCT GAAACACGGA GCACAACCGA ATGAAGTTAC ATCTAACGGC 
ACCTCTGCTC TTGCCATCGC CAAGCGGCTT GGATACATTT CAGTCATCGA TGTGCTCAAA 
CTGGTCACTG AAGAGACGGT CACTATGACC ACCACTGAAA AACATCGCAT GAGTTTCCCT 
GAAACCGTTG ATGAGATCCT GGATGTGTCT GAAGATGAAG GAATTGCACA GCTAACATTA 
GGCGAGGAGC TGCTGGGAAA CGAGGGCGCC AGGTATTTGA TGAAGATGGA GGACATAAAG 
GACCATGATG AGGATTTCCT TTCCCCCAAG AAAGTTCTAG AGTACGAGGG AGAGTTGGAG 
AATCATTCTC CTGCTATCCC GAGGATCCCG TGTGTGTCTC CTGAGACTGT CATGCTGAAA 
GAGCATGAGA TGGAGCAGCA CACTCCACTT CCATTGCCCA AAGAGTACGA CGAGGATTCT 
CTGATTCCCA GCAGTCCTGC AACTGAGACT TCAGACAATG TCAGTCCGGT TGCCAGTCCC 
ATCCACACCG GTTTTCTGGT GAGCTTCATG GTGGATGCTC GCGGCGGCTC GATGCGCGGC 
AGCAGACACA ACGGCCTCCG CGTCATCATT CCTCCTCGAA CCTGCGCCGC TCCCACACGC 
ATCACCTGCC GCCTGGTGAA GCCCCAGAAG CTGCCCACAC CGCCGCCATT AGTGGAGGGA 
GAGGGTCTCG CCAGCCGCAT CATCTCCCTT GGACCCGCCA GCATGCAGTT TTTAGGGCCT 
GTGATTGTGG AGATTCCTCA TTTTGCGGCT CTCGGTCGGG GAGATCGGGA ACTCGTGGTC 
TTGAGGAGTG AGAACGGATC GGTCTGGAAG GAACATCGTA ACCGATATGG AGATGAAGTG 
CTAGAGACCA TCCTCAACGG CATGGATGAA GATCTGGAGA GTCAGGAGGA GCTCGGGAAG 
AAACGCATCA GACGGATCAT CTCCACCGAC TTCCCGCTCT ACTTTGCGGT GGTGTCTCGC 
ATCCAGCAGG AGAACGATCT GATTGGCCCA GAAGGAGGTT TTCTGTCCAG TAAACTGGTG 
CCGATGGTCC AGGCCACCTT TCCAGAGACA GCCGTCACCA AACGCGTGCG TCTGGGACTG 
CAGGCCCAGC CGGTGCCTGA TGAGCTGGTG GCCAAGCTTT TGGGGAACCA GGCCACGTTC 
AGTCCTGTGG TGACGGTGGA GCCTCGGCGC CGGAAGTTTC ACCGTCCCAT CGGCCTAAGA 
ATCCCACTGC CACCCTCATG GAGAGAGAGC CCGCGGGACG CCGGAGAGGG AGACACCACC 
AGCCTGCGCC TGCTCTGCAG CGTCATCGGT GGAACAGCTC CTGCCCAGTG GGAAGATATA 
ACTGGCACCA CCAAACTGGC GTATTCCAAT GACTGTGCCA GTTTCACCAC CAACGTCTCT 
GCCCGATTCT GGCTGGCGGA CTGTCCTCGG ACTGCGGAGG CCGTGTCCTT TGCCAACCTC 
CTGTACCGCG AGCTGTCGGC CGTGCCGTAC ATGGCCAAGT TTGTAGTGTT TGCGAAGATG 
AATGAAGCTC GCGAGGGTCG ACTCCGCTGC TACTGCATGA CGGATGACAA GATGGACAAA 
ACCCTGGAGC AGCACGAGAA CTTCTCGGAA GTGGCTCGCA GCAGAGACAT CGAGGTGATG 
GAGGGAATGC CGCTGTATTT GGAGTGTTCA GGAAACTTGG TTCCTATAAG AAAAGCAGCC 
CAGCAGCCTC GCTGTTTCAG CTTCCAGGCC TTTAAAGACA ACAGACTGCC TGTGTCCGTC 
AAGGTGAGGG ACAGCAGTAA AGACCCCTCT GGGTTTTTGT CTTTCTTGCG CAAGTCAACC 
AAATATGAAG ACAGCCAACA CGTGCTGTGC AACCTCAACG TGACCATGCC GCCGTGCATT 
AAGATTATGG GCAGCGATGA GCGGAGGAGG ACTCTGACTC CTCTGGCTCT CAGAGAAAGA 
TACAGCGCTC TGAATGAACC GGCAATGGCA TCACTGAGCG CCATGGAAAG GACGGAGTTA 
AAGATGGCTT TAATAGCTGA ACAGCTAGGC CTGAGCTGGG CTGAGTTGGG ACGGGAACTG 
CAGTTTAATG TAGATGAAAT AAATAAGATC CGTGTGGAGA ATCCCAACTC CCTTTTAGAG 
CAGAGCTCAA CGCTGCTCAA CCTGTGGGCC GCCCGAGAGG GGAAGAGAGC CAAAATGGAC 
AGCCTGTACG TGGCCCTCAA GAGCATCGAC CGAGTGGACA TTGTGAACAT GATGGAGGGC 
CAGGGAGGAC CACCGGCGGG TCAGCAGGGG GGCTGGGAGC AGGACGCTTC TAGGTCTAGG 
CATCACGACC GAGACCATCT CTCACCTGGA ATCACAAATG GTTACGGGCT GGTGGTGCAG 
GAGGAACTAG TGTCACCGGC CTCCATGCAG TACAGTCTGC CCTCGCCACT GGGCAATGAG 
CCCTACTGGC AGGAGGTTTC CAGCATGGAG TGCGCGCCCA TCGCCACCAC TGAAGAAGAC 
ACGCTCATGG AGATGTCCGA GGTACAGGTT TGGCCCTCAG GAAATAGTCC GTCCATTGTG 
GCTGTAGAGG ACTCCTCATT GGAGTGCAGC AATGCGGACG ACTCCGAGGC GCTGCAGAAC 
CGGAGCCTGG GTCACTCCAG GAGCGGGACG CCCGGTGGGA ACGGACTCAC TCCGGGAGGT 
TTAAGTGGCT CCATCGAGCT CCTGGAGGAT CAGTCCATCG GTGCGGATTC AGACTACAGT 
GCCGCCGGGA TGCTGCGTCT GAGCGAAGGG GTCAACATCA GTGCTCTGGA CAGAGGTCAG 
AGGTCGGAGA GTTCAGAAGG GGCTGTAGGT CACCAAGTCA CCAACAACAT TAAAGACGGT 
TCCAATGGAG GTTTGGGAGA AGGAGGAGGT GACGGCACAC AGTTAGAAGA TAGCATATCT 
CTCATTTCAG GGCAACAGCG TGTGTATGCT CGAATGAGCG AGTCGCCTGG GCTGAGAAGA 
CGGGCAGATC CTGGCGAAGA CAGGTTAAGC GCAGGGTCGT TCCTGTCTTA TCTGCAGGAT 
CAGGGCTCTC CTGGTTGGCA TTCGGCTGCA GAAGCGACTC AGAACAGATT GGCGTCTTTG 
CAATGCTCCC TTCCTAGGCA GGCAATAGAG TCTATGATGT CATCGGTGCG AGCTACGGTG 
GACTCTGACT CCAAGCAGTC TTGTTTGGGC CAGGAGGCTC TGATTGAGCC AGTGCGGGAC 
ATGGGGCACT CTGAGATCAT CCATGGCCAT TATCAGGGGA CTCAGCCGTT TGAGAAAGGA 
CTCGGCTTCC CGCACAGAAC AACCAACATG CGCACTTGGG AGGACATGCG TCTGAGCGGA 
CAGGGTGATG AGGCTGAGGA TCTTCCAGGA GAGCAAGTGA GCGAGGAGCA ATTCACAGAC 
GAGCATGGCA ACATCGTCAC CAAAAAGATT GTGAGGAAAG TGGTCCGGAG AGGAAAAGGG 
GAGGAAGGGG GTCAGGAGCT GATCATTGAA GGTCTGCAGC AGGATATCAG TGAGCCTGAC 
TTTGATGGAG AACAGTTCAT GAGCTATGCC GTCCTGGGAC GTGACAGCAA GCCGGATGTT 
GTGGATGTGA AGAGAGGAGG TGCTCAGATA GTCAAATGTG CGAGTCTGCG GAGAGTAAAG 
CAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Assignment of Functional Relevance to Genes at Type 2 Diabetes-Associated Loci Through Investigation of ?-Cell Mass Deficits.
Molecular endocrinology (Baltimore, Md.)30(4)429-45(2016 Apr)
O'Hare EA,Yerges-Armstrong LM,Perry JA,Shuldiner AR,Zaghloul NA

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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ank1a cDNA ORF clone, Danio rerio(zebrafish)

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