ORF » Species Summary » Danio rerio » puf60b cDNA ORF clone

puf60b cDNA ORF clone, Danio rerio(zebrafish)

The following puf60b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the puf60b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa37762 NM_001002121.1
Latest version!		 Danio rerio poly-U binding splicing factor b (puf60b), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
ODa41006 XM_009303395.3
Latest version!		 Danio rerio poly-U binding splicing factor b (puf60b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
View Old Accession Versions >>
ODa41006 XM_009303395.2 Danio rerio poly-U binding splicing factor b (puf60b), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    puf60b ( NM_001002121.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001002121.1 Danio rerio poly-U binding splicing factor b (puf60b), mRNA.
    Price & Availability↑
    CloneID ODa37762
    Clone ID Related Accession (Same CDS sequence) NM_001002121.1
    Accession Version NM_001002121.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1509bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-30
    Organism Danio rerio(zebrafish)
    Product poly(U)-binding-splicing factor PUF60-B
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC071467.1. ##Evidence-Data-START## Transcript exon combination :: BC071467.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAGAATG GACAAGGCAC AGGGTCAAAG CTTGGATTAC CGCCTCTTAC TCCTGAGCAA 
    CAGGAGGCGC TACAGAAGGC TAAGAAGTAT GCCATGGAAC AGAGCATCAA GAGTGTTTTA 
    GTGAAACAGA CCATCGCCCA CCAGCAGCAG CAGCTCACAA ACTTACAGGT GGCAGCACAG 
    AGGCAGCGAG CTCTGGCCAT CATGTGTCGT GTGTATGTGG GCTCCATTTA TTACGAGCTG 
    GGGGAAGATA CCATCCGGCA GGCCTTCGCA CCCTTTGGTC CCATTAAGAG CATCGATATG 
    TCTTGGGATT CTGTCACCAT GAAACATAAG GGCTTTGCCT TTGTGGAGTA TGAGGTTCCT 
    GAAGCGGCTC AGCTGGCCTT GGAGCAGATG AACTCTGTCA TGCTGGGTGG GAGGAACATT 
    AAGGTGGGTC GGCCAGGTAG CATTGGACAG GCGCAGCCTA TCATTGAACA ACTGGCAGAG 
    GAGGCACGTG CCTACAACCG AATCTACGTA GCCTCTATCC ACCCTGATCT GTCTGACGAC 
    GATATCAAGA GCGTCTTTGA GGCCTTTGGA AAGATTAAAA GTTGTATGCT GGCACGAGAA 
    CCCACCACGG GAAAGCACAA GGGCTTCGGA TTTATCGAAT ATGAAAAGCC TCAGTCATCG 
    CTGGATGCTG TCTCATCCAT GAACCTGTTT GACCTCGGCG GTCAGTATCT AAGAGTGGGC 
    AAGGCTGTTA CTCCACCCAT GCCTCTGCTA ACACCTACAA CTCCCGGAGG TTTGCCCCCT 
    GCTGCAGCTG TGGCCGCTGC TGCTGCCACC GCCAAGATTA CTGCCCAGGA GGCTGTTGCT 
    GGGACAGTGC TGAGTTCTCT TGCCTCACCA GCTCACATTC TCAGTCAGCA AATGGGACTT 
    CCACAGGCTG TACTGGCTGC CCAGGCCCCT GGCATTATCA CAGGTGTGAC ACCTGCTCGC 
    CCCACTCTCC CTGTAGTCCC GCAGGTGGGT TTGGTCAACC CAGTTCTGGC ATCCCCACCA 
    GTCACAGCAC CTGCTTCTAT GGGAACACCA ACATCTGCAG TGCAATTGCA CACAGAAGTG 
    AAGAGAGAGG AAGACAGCAG GCGAACTGCA GAAGATCACA GTGCACCGGT TGGCAATGGA 
    CAAGACAGTG AACTGGAGCG TCTCAGCGTC AGCGCAAGTG CAGGAAGACA AGCGGCCATT 
    CAGTCCAAGT CAACGGTTAT GGTGTTGCGC AACATGGTTG GTCCAGAGGA CATTGACGAT 
    GACCTGGAAG GGGAAGTGAT GGAGGAATGT GGGAAGTACG GGGCTGTGAA CCGGGTTATC 
    ATATACCAGG AGCGACAGGG TGAGGAGGAT GATGCCGAGA TAATAGTGAA GATCTTCGTG 
    GAGTTTTCTG ATGCTGGTGA AATGAACAAG GCCATCCAAG CTCTCAACAA CCGCTGGTTT 
    GCAGGTCGCA AGGTTGTTGC CGAGTTGTAC GATCAAGATC GTTTTAATAG CAGTGACCTC 
    ACTGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001002121.1
    CDS118..1626
    Translation

    Target ORF information:

    RefSeq Version NM_001002121.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio poly-U binding splicing factor b (puf60b), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 NM_001002121.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    ATGGAGAATG GACAAGGCAC AGGGTCAAAG CTTGGATTAC CGCCTCTTAC TCCTGAGCAA 
    CAGGAGGCGC TACAGAAGGC TAAGAAGTAT GCCATGGAAC AGAGCATCAA GAGTGTTTTA 
    GTGAAACAGA CCATCGCCCA CCAGCAGCAG CAGCTCACAA ACTTACAGGT GGCAGCACAG 
    AGGCAGCGAG CTCTGGCCAT CATGTGTCGT GTGTATGTGG GCTCCATTTA TTACGAGCTG 
    GGGGAAGATA CCATCCGGCA GGCCTTCGCA CCCTTTGGTC CCATTAAGAG CATCGATATG 
    TCTTGGGATT CTGTCACCAT GAAACATAAG GGCTTTGCCT TTGTGGAGTA TGAGGTTCCT 
    GAAGCGGCTC AGCTGGCCTT GGAGCAGATG AACTCTGTCA TGCTGGGTGG GAGGAACATT 
    AAGGTGGGTC GGCCAGGTAG CATTGGACAG GCGCAGCCTA TCATTGAACA ACTGGCAGAG 
    GAGGCACGTG CCTACAACCG AATCTACGTA GCCTCTATCC ACCCTGATCT GTCTGACGAC 
    GATATCAAGA GCGTCTTTGA GGCCTTTGGA AAGATTAAAA GTTGTATGCT GGCACGAGAA 
    CCCACCACGG GAAAGCACAA GGGCTTCGGA TTTATCGAAT ATGAAAAGCC TCAGTCATCG 
    CTGGATGCTG TCTCATCCAT GAACCTGTTT GACCTCGGCG GTCAGTATCT AAGAGTGGGC 
    AAGGCTGTTA CTCCACCCAT GCCTCTGCTA ACACCTACAA CTCCCGGAGG TTTGCCCCCT 
    GCTGCAGCTG TGGCCGCTGC TGCTGCCACC GCCAAGATTA CTGCCCAGGA GGCTGTTGCT 
    GGGACAGTGC TGAGTTCTCT TGCCTCACCA GCTCACATTC TCAGTCAGCA AATGGGACTT 
    CCACAGGCTG TACTGGCTGC CCAGGCCCCT GGCATTATCA CAGGTGTGAC ACCTGCTCGC 
    CCCACTCTCC CTGTAGTCCC GCAGGTGGGT TTGGTCAACC CAGTTCTGGC ATCCCCACCA 
    GTCACAGCAC CTGCTTCTAT GGGAACACCA ACATCTGCAG TGCAATTGCA CACAGAAGTG 
    AAGAGAGAGG AAGACAGCAG GCGAACTGCA GAAGATCACA GTGCACCGGT TGGCAATGGA 
    CAAGACAGTG AACTGGAGCG TCTCAGCGTC AGCGCAAGTG CAGGAAGACA AGCGGCCATT 
    CAGTCCAAGT CAACGGTTAT GGTGTTGCGC AACATGGTTG GTCCAGAGGA CATTGACGAT 
    GACCTGGAAG GGGAAGTGAT GGAGGAATGT GGGAAGTACG GGGCTGTGAA CCGGGTTATC 
    ATATACCAGG AGCGACAGGG TGAGGAGGAT GATGCCGAGA TAATAGTGAA GATCTTCGTG 
    GAGTTTTCTG ATGCTGGTGA AATGAACAAG GCCATCCAAG CTCTCAACAA CCGCTGGTTT 
    GCAGGTCGCA AGGTTGTTGC CGAGTTGTAC GATCAAGATC GTTTTAATAG CAGTGACCTC 
    ACTGCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    puf60b ( XM_009303395.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301670.1 Danio rerio poly-U binding splicing factor b (puf60b), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa41006
    Clone ID Related Accession (Same CDS sequence) XM_009303395.2 , XM_009303395.3
    Accession Version XM_009303395.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1563bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product poly(U)-binding-splicing factor PUF60-B isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303395.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGATGGAGA ATGGACAAGG CACAGGGTCA AAGCTTGGAT TACCGCCTCT TACTCCTGAG 
    CAACAGGAGG CGCTACAGAA GGCTAAGAAG TATGCCATGG AACAGAGCAT CAAGAGTGTT 
    TTAGTGAAAC AGACCATCGC CCACCAGCAG CAGCAGCTCA CAAACTTACA GATGGCAGCA 
    GTGACAATGG GCTTTGGAGA TCCTCTCTCA TCTTTACAAT CGGTGGCAGC ACAGAGGCAG 
    CGAGCTCTGG CCATCATGTG TCGTGTGTAT GTGGGCTCCA TTTATTACGA GCTGGGGGAA 
    GATACCATCC GGCAGGCCTT CGCACCCTTT GGTCCCATTA AGAGCATCGA TATGTCTTGG 
    GATTCTGTCA CCATGAAACA TAAGGGCTTT GCCTTTGTGG AGTATGAGGT TCCTGAAGCG 
    GCTCAGCTGG CCTTGGAGCA GATGAACTCT GTCATGCTGG GTGGGAGGAA CATTAAGGTG 
    GGTCGGCCAG GTAGCATTGG ACAGGCGCAG CCTATCATTG AACAACTGGC AGAGGAGGCA 
    CGTGCCTACA ACCGAATCTA CGTAGCCTCT ATCCACCCTG ATCTGTCTGA CGACGATATC 
    AAGAGCGTCT TTGAGGCCTT TGGAAAGATT AAAAGTTGTA TGCTGGCACG AGAACCCACC 
    ACGGGAAAGC ACAAGGGCTT CGGATTTATC GAATATGAAA AGCCTCAGTC ATCGCTGGAT 
    GCTGTCTCAT CCATGAACCT GTTTGACCTC GGCGGTCAGT ATCTAAGAGT GGGCAAGGCT 
    GTTACTCCAC CCATGCCTCT GCTAACACCT ACAACTCCCG GAGGTTTGCC CCCTGCTGCA 
    GCTGTGGCCG CTGCTGCTGC CACCGCCAAG ATTACTGCCC AGGAGGCTGT TGCTGGGACA 
    GTGCTGAGTT CTCTTGCCTC ACCAGCTCAC ATTCTCAGTC AGCAAATGGG ACTTCCACAG 
    GCTGTACTGG CTGCCCAGGC CCCTGGCATT ATCACAGGTG TGACACCTGC TCGCCCCACT 
    CTCCCTGTAG TCCCGCAGGT GGGTTTGGTC AACCCAGTTC TGGCATCCCC ACCAGTCACA 
    GCACCTGCTT CTATGGGAAC ACCAACATCT GCAGTGCAAT TGCACACAGA AGTGAAGAGA 
    GAGGAAGACA GCAGGCGAAC TGCAGAAGAT CACAGTGCAC CGGTTGGCAA TGGACAAGAC 
    AGTGAACTGG AGCGTCTCAG CGTCAGCGCA AGTGCAGGAA GACAAGCGGC CATTCAGTCC 
    AAGTCAACGG TTATGGTGTT GCGCAACATG GTTGGTCCAG AGGACATTGA CGATGACCTG 
    GAAGGGGAAG TGATGGAGGA ATGTGGGAAG TACGGGGCTG TGAACCGGGT TATCATATAC 
    CAGGAGCGAC AGGGTGAGGA GGATGATGCC GAGATAATAG TGAAGATCTT CGTGGAGTTT 
    TCTGATGCTG GTGAAATGAA CAAGGCCATC CAAGCTCTCA ACAACCGCTG GTTTGCAGGT 
    CGCAAGGTTG TTGCCGAGTT GTACGATCAA GATCGTTTTA ATAGCAGTGA CCTCACTGCG 
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301670.1
    CDS210..1772
    Translation

    Target ORF information:

    RefSeq Version XM_009303395.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio poly-U binding splicing factor b (puf60b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009303395.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGATGGAGA ATGGACAAGG CACAGGGTCA AAGCTTGGAT TACCGCCTCT TACTCCTGAG 
    CAACAGGAGG CGCTACAGAA GGCTAAGAAG TATGCCATGG AACAGAGCAT CAAGAGTGTT 
    TTAGTGAAAC AGACCATCGC CCACCAGCAG CAGCAGCTCA CAAACTTACA GATGGCAGCA 
    GTGACAATGG GCTTTGGAGA TCCTCTCTCA TCTTTACAAT CGGTGGCAGC ACAGAGGCAG 
    CGAGCTCTGG CCATCATGTG TCGTGTGTAT GTGGGCTCCA TTTATTACGA GCTGGGGGAA 
    GATACCATCC GGCAGGCCTT CGCACCCTTT GGTCCCATTA AGAGCATCGA TATGTCTTGG 
    GATTCTGTCA CCATGAAACA TAAGGGCTTT GCCTTTGTGG AGTATGAGGT TCCTGAAGCG 
    GCTCAGCTGG CCTTGGAGCA GATGAACTCT GTCATGCTGG GTGGGAGGAA CATTAAGGTG 
    GGTCGGCCAG GTAGCATTGG ACAGGCGCAG CCTATCATTG AACAACTGGC AGAGGAGGCA 
    CGTGCCTACA ACCGAATCTA CGTAGCCTCT ATCCACCCTG ATCTGTCTGA CGACGATATC 
    AAGAGCGTCT TTGAGGCCTT TGGAAAGATT AAAAGTTGTA TGCTGGCACG AGAACCCACC 
    ACGGGAAAGC ACAAGGGCTT CGGATTTATC GAATATGAAA AGCCTCAGTC ATCGCTGGAT 
    GCTGTCTCAT CCATGAACCT GTTTGACCTC GGCGGTCAGT ATCTAAGAGT GGGCAAGGCT 
    GTTACTCCAC CCATGCCTCT GCTAACACCT ACAACTCCCG GAGGTTTGCC CCCTGCTGCA 
    GCTGTGGCCG CTGCTGCTGC CACCGCCAAG ATTACTGCCC AGGAGGCTGT TGCTGGGACA 
    GTGCTGAGTT CTCTTGCCTC ACCAGCTCAC ATTCTCAGTC AGCAAATGGG ACTTCCACAG 
    GCTGTACTGG CTGCCCAGGC CCCTGGCATT ATCACAGGTG TGACACCTGC TCGCCCCACT 
    CTCCCTGTAG TCCCGCAGGT GGGTTTGGTC AACCCAGTTC TGGCATCCCC ACCAGTCACA 
    GCACCTGCTT CTATGGGAAC ACCAACATCT GCAGTGCAAT TGCACACAGA AGTGAAGAGA 
    GAGGAAGACA GCAGGCGAAC TGCAGAAGAT CACAGTGCAC CGGTTGGCAA TGGACAAGAC 
    AGTGAACTGG AGCGTCTCAG CGTCAGCGCA AGTGCAGGAA GACAAGCGGC CATTCAGTCC 
    AAGTCAACGG TTATGGTGTT GCGCAACATG GTTGGTCCAG AGGACATTGA CGATGACCTG 
    GAAGGGGAAG TGATGGAGGA ATGTGGGAAG TACGGGGCTG TGAACCGGGT TATCATATAC 
    CAGGAGCGAC AGGGTGAGGA GGATGATGCC GAGATAATAG TGAAGATCTT CGTGGAGTTT 
    TCTGATGCTG GTGAAATGAA CAAGGCCATC CAAGCTCTCA ACAACCGCTG GTTTGCAGGT 
    CGCAAGGTTG TTGCCGAGTT GTACGATCAA GATCGTTTTA ATAGCAGTGA CCTCACTGCG 
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    puf60b ( XM_009303395.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009301670.1 Danio rerio poly-U binding splicing factor b (puf60b), transcript variant X1, mRNA.
    Price & Availability↑
    CloneID ODa41006
    Clone ID Related Accession (Same CDS sequence) XM_009303395.2 , XM_009303395.3
    Accession Version XM_009303395.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1563bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product poly(U)-binding-splicing factor PUF60-B isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303395.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## 

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGATGGAGA ATGGACAAGG CACAGGGTCA AAGCTTGGAT TACCGCCTCT TACTCCTGAG 
    CAACAGGAGG CGCTACAGAA GGCTAAGAAG TATGCCATGG AACAGAGCAT CAAGAGTGTT 
    TTAGTGAAAC AGACCATCGC CCACCAGCAG CAGCAGCTCA CAAACTTACA GATGGCAGCA 
    GTGACAATGG GCTTTGGAGA TCCTCTCTCA TCTTTACAAT CGGTGGCAGC ACAGAGGCAG 
    CGAGCTCTGG CCATCATGTG TCGTGTGTAT GTGGGCTCCA TTTATTACGA GCTGGGGGAA 
    GATACCATCC GGCAGGCCTT CGCACCCTTT GGTCCCATTA AGAGCATCGA TATGTCTTGG 
    GATTCTGTCA CCATGAAACA TAAGGGCTTT GCCTTTGTGG AGTATGAGGT TCCTGAAGCG 
    GCTCAGCTGG CCTTGGAGCA GATGAACTCT GTCATGCTGG GTGGGAGGAA CATTAAGGTG 
    GGTCGGCCAG GTAGCATTGG ACAGGCGCAG CCTATCATTG AACAACTGGC AGAGGAGGCA 
    CGTGCCTACA ACCGAATCTA CGTAGCCTCT ATCCACCCTG ATCTGTCTGA CGACGATATC 
    AAGAGCGTCT TTGAGGCCTT TGGAAAGATT AAAAGTTGTA TGCTGGCACG AGAACCCACC 
    ACGGGAAAGC ACAAGGGCTT CGGATTTATC GAATATGAAA AGCCTCAGTC ATCGCTGGAT 
    GCTGTCTCAT CCATGAACCT GTTTGACCTC GGCGGTCAGT ATCTAAGAGT GGGCAAGGCT 
    GTTACTCCAC CCATGCCTCT GCTAACACCT ACAACTCCCG GAGGTTTGCC CCCTGCTGCA 
    GCTGTGGCCG CTGCTGCTGC CACCGCCAAG ATTACTGCCC AGGAGGCTGT TGCTGGGACA 
    GTGCTGAGTT CTCTTGCCTC ACCAGCTCAC ATTCTCAGTC AGCAAATGGG ACTTCCACAG 
    GCTGTACTGG CTGCCCAGGC CCCTGGCATT ATCACAGGTG TGACACCTGC TCGCCCCACT 
    CTCCCTGTAG TCCCGCAGGT GGGTTTGGTC AACCCAGTTC TGGCATCCCC ACCAGTCACA 
    GCACCTGCTT CTATGGGAAC ACCAACATCT GCAGTGCAAT TGCACACAGA AGTGAAGAGA 
    GAGGAAGACA GCAGGCGAAC TGCAGAAGAT CACAGTGCAC CGGTTGGCAA TGGACAAGAC 
    AGTGAACTGG AGCGTCTCAG CGTCAGCGCA AGTGCAGGAA GACAAGCGGC CATTCAGTCC 
    AAGTCAACGG TTATGGTGTT GCGCAACATG GTTGGTCCAG AGGACATTGA CGATGACCTG 
    GAAGGGGAAG TGATGGAGGA ATGTGGGAAG TACGGGGCTG TGAACCGGGT TATCATATAC 
    CAGGAGCGAC AGGGTGAGGA GGATGATGCC GAGATAATAG TGAAGATCTT CGTGGAGTTT 
    TCTGATGCTG GTGAAATGAA CAAGGCCATC CAAGCTCTCA ACAACCGCTG GTTTGCAGGT 
    CGCAAGGTTG TTGCCGAGTT GTACGATCAA GATCGTTTTA ATAGCAGTGA CCTCACTGCG 
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301670.1
    CDS253..1815
    Translation

    Target ORF information:

    RefSeq Version XM_009303395.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio poly-U binding splicing factor b (puf60b), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    		 XM_009303395.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    ATGATGGAGA ATGGACAAGG CACAGGGTCA AAGCTTGGAT TACCGCCTCT TACTCCTGAG 
    CAACAGGAGG CGCTACAGAA GGCTAAGAAG TATGCCATGG AACAGAGCAT CAAGAGTGTT 
    TTAGTGAAAC AGACCATCGC CCACCAGCAG CAGCAGCTCA CAAACTTACA GATGGCAGCA 
    GTGACAATGG GCTTTGGAGA TCCTCTCTCA TCTTTACAAT CGGTGGCAGC ACAGAGGCAG 
    CGAGCTCTGG CCATCATGTG TCGTGTGTAT GTGGGCTCCA TTTATTACGA GCTGGGGGAA 
    GATACCATCC GGCAGGCCTT CGCACCCTTT GGTCCCATTA AGAGCATCGA TATGTCTTGG 
    GATTCTGTCA CCATGAAACA TAAGGGCTTT GCCTTTGTGG AGTATGAGGT TCCTGAAGCG 
    GCTCAGCTGG CCTTGGAGCA GATGAACTCT GTCATGCTGG GTGGGAGGAA CATTAAGGTG 
    GGTCGGCCAG GTAGCATTGG ACAGGCGCAG CCTATCATTG AACAACTGGC AGAGGAGGCA 
    CGTGCCTACA ACCGAATCTA CGTAGCCTCT ATCCACCCTG ATCTGTCTGA CGACGATATC 
    AAGAGCGTCT TTGAGGCCTT TGGAAAGATT AAAAGTTGTA TGCTGGCACG AGAACCCACC 
    ACGGGAAAGC ACAAGGGCTT CGGATTTATC GAATATGAAA AGCCTCAGTC ATCGCTGGAT 
    GCTGTCTCAT CCATGAACCT GTTTGACCTC GGCGGTCAGT ATCTAAGAGT GGGCAAGGCT 
    GTTACTCCAC CCATGCCTCT GCTAACACCT ACAACTCCCG GAGGTTTGCC CCCTGCTGCA 
    GCTGTGGCCG CTGCTGCTGC CACCGCCAAG ATTACTGCCC AGGAGGCTGT TGCTGGGACA 
    GTGCTGAGTT CTCTTGCCTC ACCAGCTCAC ATTCTCAGTC AGCAAATGGG ACTTCCACAG 
    GCTGTACTGG CTGCCCAGGC CCCTGGCATT ATCACAGGTG TGACACCTGC TCGCCCCACT 
    CTCCCTGTAG TCCCGCAGGT GGGTTTGGTC AACCCAGTTC TGGCATCCCC ACCAGTCACA 
    GCACCTGCTT CTATGGGAAC ACCAACATCT GCAGTGCAAT TGCACACAGA AGTGAAGAGA 
    GAGGAAGACA GCAGGCGAAC TGCAGAAGAT CACAGTGCAC CGGTTGGCAA TGGACAAGAC 
    AGTGAACTGG AGCGTCTCAG CGTCAGCGCA AGTGCAGGAA GACAAGCGGC CATTCAGTCC 
    AAGTCAACGG TTATGGTGTT GCGCAACATG GTTGGTCCAG AGGACATTGA CGATGACCTG 
    GAAGGGGAAG TGATGGAGGA ATGTGGGAAG TACGGGGCTG TGAACCGGGT TATCATATAC 
    CAGGAGCGAC AGGGTGAGGA GGATGATGCC GAGATAATAG TGAAGATCTT CGTGGAGTTT 
    TCTGATGCTG GTGAAATGAA CAAGGCCATC CAAGCTCTCA ACAACCGCTG GTTTGCAGGT 
    CGCAAGGTTG TTGCCGAGTT GTACGATCAA GATCGTTTTA ATAGCAGTGA CCTCACTGCG 
    TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

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