bsk146 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	bsk146
Entrez Gene ID	405767
Full Name	brain specific kinase 146
Synonyms	sbk1
General protein information	Preferred Names brain specific kinase 146 Names serine/threonine-protein kinase SBK1 SH3-binding kinase 1 brain-specific protein kinase BSK146 protein kinase Bsk146
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009306356.2	XP_009304631.1	serine/threonine-protein kinase SBK1 isoform X1
XM_009306356.3	XP_009304631.1	serine/threonine-protein kinase SBK1 isoform X1
NM_212841.1	NP_998006.1	serine/threonine-protein kinase SBK1
NM_212841.2	NP_998006.2	serine/threonine-protein kinase SBK1

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC791840	NP_001189435.1
Drosophila melanogaster (fruit fly)	CG11221	NP_609070.1
Caenorhabditis elegans (roundworm)	C01C4.3	NP_741754.2
Danio rerio (zebrafish)	LOC557524	XP_684216.2
Xenopus tropicalis (tropical clawed frog)	LOC100495913	XP_002935965.1
Danio rerio (zebrafish)	bsk146	NP_998006.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP009934	XP_319058.4

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Identification of single-nucleotide polymorphism markers associated with cortisol response to crowding in rainbow trout.
Liu S, Vallejo RL, Gao G, Palti Y, Weber GM, Hernandez A, Rexroad CE 3rd
Marine biotechnology (New York, N.Y.)17(3)328-37(2015 Jun)

Gene expression profiling in zebrafish embryos exposed to diclofenac, an environmental toxicant.
De Felice B, Copia L, Guida M
Molecular biology reports39(3)2119-28(2012 Mar)

Expression and characterization of a brain-specific protein kinase BSK146 from zebrafish.
Chou CM, Chen YC, Lee MT, Chen GD, Lu IC, Chen ST, Huang CJ
Biochemical and biophysical research communications340(3)767-75(2006 Feb)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

BSK146 is a brain-specific protein kinase zebrafish
Title: Expression and characterization of a brain-specific protein kinase BSK146 from zebrafish.

The following bsk146 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the bsk146 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa33030	NM_212841.2 Latest version!	Danio rerio brain specific kinase 146 (bsk146), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa37650	NM_212841.1 Latest version!	Danio rerio brain specific kinase 146 (bsk146), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
ODa33030	XM_009306356.3 Latest version!	Danio rerio brain specific kinase 146 (bsk146), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
View Old Accession Versions >>
ODa33030	XM_009306356.2	Danio rerio brain specific kinase 146 (bsk146), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

bsk146 ( NM_212841.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_998006.2 Danio rerio brain specific kinase 146 (bsk146), mRNA.

Price & Availability↑

CloneID	ODa33030
Clone ID Related Accession (Same CDS sequence)	XM_009306356.2 , XM_009306356.3 , NM_212841.2
Accession Version	NM_212841.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-12-22
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase SBK1
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX000348.22. On or before Apr 14, 2018 this sequence version replaced XM_009306356.3, NM_212841.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: AF265347.1, BC162392.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476771, SAMEA4476785 [ECO:0000348] ##Evidence-Data-END## COMPLETENESS: complete on the 3' end.

ATGAGCTCGT CTCCGGTGGT TTCCCATGAC ATTCTGGAGG AGCTGCAGCT CTACACCGCT 
CAGAACCTGG AGAAGCTGGA GGTCAATAAA TACTATGAGG TGATCCGGGA GCTCGGGAAG 
GGCACTTACG GGAAGGTGGA CCTCGTCATT CATAAGATCA GAGGTAGCAA AATGGCTCTG 
AAGTTTCTGA AGAAGAAAAG CACCAAGCTG AAGAGCTTCT TGCGGGAGTA CAGCATCTCT 
CTCTATCTCT CTCCATGTCC CTTCATCATC AACATGTTCG GCATCGCCTT TGAGACAGAC 
GAGTATTATG TGTTTGCGCA GGAATACGCT CCTTCTGGGG ACCTGTTTGA CATCATTCCA 
CCACAGGTGG GTCTGCCAGA GCCGGTGGCG AAGCGCTGTG TGCATCAGGT GGCCATCGCT 
CTCGAATACC TGCACTCCAA AAAACTGGTG CACCGAGACA TCAAACCCGA GAACATCCTA 
ATCTTCGACA AGGAGTGCCG TAAGGTCAAG CTGTCAGACT TCGGGATGGC GCGGCGCGCC 
GGTTCACCGG TCAAGCGTGT TAGCGGCACA ATCCCATACA CGGCTCCAGA ACTCTGCGAT 
ACGTCCAAAC ACGACGGCTT CTGCGTGGAC TACAGCACCG ATGTGTGGGC GTTCGGAGTG 
CTGCTGTTCT GCATGCTAAC CGGGAACTTT CCATGGGAAA AAGCGATGCC GTCTGATACT 
TTCTACGAGG AGTTTGTGCG CTGGCAGAAG CGGCGGACGG GTGCGGTTCC GTCACAATGG 
CGTCGATTTA CAGACGAATC CCTGCGCATG TTTCGAAAGC TCCTAGCATT GGAGCAGGAA 
CGCAGATGCA GCGTCAAGGA GGTTTTCGCA CATTTAGGCC ACCGCTGGAT GCTTGATGGA 
ACTAGCGGAA ACCATCATCA ATCCGTGCTC AACTCTTCAT CAGAGGAAGA CGAATTGCTT 
GTGGATCGTA TGAAGCAGCA GACCTTATCT CCGACAGCTA ACACGAGCAA TGCTATTGAG 
CCCGGATCGG CTAACCACTT CACATCTGTG TCCACCAATA GCTCGGTGTC TTCCACTAAC 
AGCTATGAGC GTTCGGCTAG AGACAGTCCT CCGACTAGCA GGATCCTGGT CACGACGCCT 
ATAGAAATCT GCGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998006.2
CDS	188..1345
Translation	MSSSPVVSHDILEELQLYTAQNLEKLEVNKYYEVIRELGKGTYGKVDLVIHKIRGSKMALKFLKKKSTKLKSFLREYSISLYLSPCPFIINMFGIAFETDEYYVFAQEYAPSGDLFDIIPPQVGLPEPVAKRCVHQVAIALEYLHSKKLVHRDIKPENILIFDKECRKVKLSDFGMARRAGSPVKRVSGTIPYTAPELCDTSKHDGFCVDYSTDVWAFGVLLFCMLTGNFPWEKAMPSDTFYEEFVRWQKRRTGAVPSQWRRFTDESLRMFRKLLALEQERRCSVKEVFAHLGHRWMLDGTSGNHHQSVLNSSSEEDELLVDRMKQQTLSPTANTSNAIEPGSANHFTSVSTNSSVSSTNSYERSARDSPPTSRILVTTPIEICV

Target ORF information:

RefSeq Version	NM_212841.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio brain specific kinase 146 (bsk146), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212841.2

ORF Insert Sequence:

ATGAGCTCGT CTCCGGTGGT TTCCCATGAC ATTCTGGAGG AGCTGCAGCT CTACACCGCT 
CAGAACCTGG AGAAGCTGGA GGTCAATAAA TACTATGAGG TGATCCGGGA GCTCGGGAAG 
GGCACTTACG GGAAGGTGGA CCTCGTCATT CATAAGATCA GAGGTAGCAA AATGGCTCTG 
AAGTTTCTGA AGAAGAAAAG CACCAAGCTG AAGAGCTTCT TGCGGGAGTA CAGCATCTCT 
CTCTATCTCT CTCCATGTCC CTTCATCATC AACATGTTCG GCATCGCCTT TGAGACAGAC 
GAGTATTATG TGTTTGCGCA GGAATACGCT CCTTCTGGGG ACCTGTTTGA CATCATTCCA 
CCACAGGTGG GTCTGCCAGA GCCGGTGGCG AAGCGCTGTG TGCATCAGGT GGCCATCGCT 
CTCGAATACC TGCACTCCAA AAAACTGGTG CACCGAGACA TCAAACCCGA GAACATCCTA 
ATCTTCGACA AGGAGTGCCG TAAGGTCAAG CTGTCAGACT TCGGGATGGC GCGGCGCGCC 
GGTTCACCGG TCAAGCGTGT TAGCGGCACA ATCCCATACA CGGCTCCAGA ACTCTGCGAT 
ACGTCCAAAC ACGACGGCTT CTGCGTGGAC TACAGCACCG ATGTGTGGGC GTTCGGAGTG 
CTGCTGTTCT GCATGCTAAC CGGGAACTTT CCATGGGAAA AAGCGATGCC GTCTGATACT 
TTCTACGAGG AGTTTGTGCG CTGGCAGAAG CGGCGGACGG GTGCGGTTCC GTCACAATGG 
CGTCGATTTA CAGACGAATC CCTGCGCATG TTTCGAAAGC TCCTAGCATT GGAGCAGGAA 
CGCAGATGCA GCGTCAAGGA GGTTTTCGCA CATTTAGGCC ACCGCTGGAT GCTTGATGGA 
ACTAGCGGAA ACCATCATCA ATCCGTGCTC AACTCTTCAT CAGAGGAAGA CGAATTGCTT 
GTGGATCGTA TGAAGCAGCA GACCTTATCT CCGACAGCTA ACACGAGCAA TGCTATTGAG 
CCCGGATCGG CTAACCACTT CACATCTGTG TCCACCAATA GCTCGGTGTC TTCCACTAAC 
AGCTATGAGC GTTCGGCTAG AGACAGTCCT CCGACTAGCA GGATCCTGGT CACGACGCCT 
ATAGAAATCT GCGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bsk146 ( NM_212841.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_998006.1 Danio rerio brain specific kinase 146 (bsk146), mRNA.

Price & Availability↑

CloneID	ODa37650
Clone ID Related Accession (Same CDS sequence)	NM_212841.1
Accession Version	NM_212841.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-11-25
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase SBK1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF265347.1. ##Evidence-Data-START## Transcript exon combination :: AF265347.1, BC162392.1 [ECO:0000332] ##Evidence-Data-END##

ATGAGCTCGT CTCCGGTGGT TTCCCGTGAC ATTCTGGAGG AGCTGCAGCT CTACACCGCT 
CAGAACCTGG AGAAGCTGGA GGTCAATAAA TACTATGAGG TGATCCGGGA GCTCGGGAAG 
GGCACTTACG GGAAGGTGGA CCTCGTCATT CATAAGATCA GAGGTAGCAA AATGGCTCTG 
AAGTTTCTGA AGAAGAAAAG CACCAAGCTG AAGAGCTTCC TGCGGGAGTA CAGCATCTCT 
CTCTATCTCT CTCCGTGTCC CTTCATCATC AACATGTTCG GCATCGCCTT TGAGACAGAC 
GAGTATTATG TGTTTGCGCA GGAATACGCT CCTTCTGGGG ACCTGTTTGA CATCATTCCA 
CCACAGGTGG GTCTGCCGGA GCCGGTGGCG AAGCGCTGTG TGCATCAGGT GGCCATCGCT 
CTCGAATACC TGCACTCCAA AAAACTGGTG CACCGAGACA TCAAACCCGA GAACATCCTA 
ATCTTCGACA AGGAGTGCCG TAAGGTCAAG CTGTCAGACT TCGGGATGAC GCGGCGCGCC 
GGTTCACCGG TCAAACGTGT TAGCGGCACA ATCCCATACA CGGCTCCAGA ACTCTGCGAT 
ACGTCCAAAC ACGACGGCTT CTGCGTGGAC TACAGCACCG ATGTGTGGGC GTTCGGAGTG 
CTGCTGTTCT GCATGCTAAC CGGGAACTTT CCATGGGAAA AAGCGATGCC GTCTGATACT 
TTCTACGAGG AGTTCGTGCG CTGGCAGAAG CGGCGGACGG GTGCGGTTCC GTCACAATGG 
CGTCGATTTA CAGACGAATC CCTGCGCATG TTTCGAAAGC TCCTAGCACT GGAGCAGGAA 
CGCAGATGCA GCGTCAAGGA GGTTTTCGCA CATTTAGGCC ACCGCTGGAT GCTTGATGGA 
ACTAGCGGAA ACCATCATCA ATCCGTGCTC AACTCTTCAT CGGAGGAAGA CGAGTTGCTT 
GTGGATCGTA TGAAGCAGCA GACCTTATCT CCGACAGCTA ACACGAGCAA TGCTATTGAG 
CCCGGATCGG CTAACCACTT CACATCTATG TCCACCAATA GCTCGGTGTC TTCCACTAAC 
AGCTATGAGC GTTCGGCTAG AGACAGTCCT CCGACTAGCA GGATCCTGGT CACGACGCCT 
ATAGAAATCT GCGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_998006.1
CDS	188..1345
Translation	MSSSPVVSRDILEELQLYTAQNLEKLEVNKYYEVIRELGKGTYGKVDLVIHKIRGSKMALKFLKKKSTKLKSFLREYSISLYLSPCPFIINMFGIAFETDEYYVFAQEYAPSGDLFDIIPPQVGLPEPVAKRCVHQVAIALEYLHSKKLVHRDIKPENILIFDKECRKVKLSDFGMTRRAGSPVKRVSGTIPYTAPELCDTSKHDGFCVDYSTDVWAFGVLLFCMLTGNFPWEKAMPSDTFYEEFVRWQKRRTGAVPSQWRRFTDESLRMFRKLLALEQERRCSVKEVFAHLGHRWMLDGTSGNHHQSVLNSSSEEDELLVDRMKQQTLSPTANTSNAIEPGSANHFTSMSTNSSVSSTNSYERSARDSPPTSRILVTTPIEICV

Target ORF information:

RefSeq Version	NM_212841.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio brain specific kinase 146 (bsk146), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_212841.1

ORF Insert Sequence:

ATGAGCTCGT CTCCGGTGGT TTCCCGTGAC ATTCTGGAGG AGCTGCAGCT CTACACCGCT 
CAGAACCTGG AGAAGCTGGA GGTCAATAAA TACTATGAGG TGATCCGGGA GCTCGGGAAG 
GGCACTTACG GGAAGGTGGA CCTCGTCATT CATAAGATCA GAGGTAGCAA AATGGCTCTG 
AAGTTTCTGA AGAAGAAAAG CACCAAGCTG AAGAGCTTCC TGCGGGAGTA CAGCATCTCT 
CTCTATCTCT CTCCGTGTCC CTTCATCATC AACATGTTCG GCATCGCCTT TGAGACAGAC 
GAGTATTATG TGTTTGCGCA GGAATACGCT CCTTCTGGGG ACCTGTTTGA CATCATTCCA 
CCACAGGTGG GTCTGCCGGA GCCGGTGGCG AAGCGCTGTG TGCATCAGGT GGCCATCGCT 
CTCGAATACC TGCACTCCAA AAAACTGGTG CACCGAGACA TCAAACCCGA GAACATCCTA 
ATCTTCGACA AGGAGTGCCG TAAGGTCAAG CTGTCAGACT TCGGGATGAC GCGGCGCGCC 
GGTTCACCGG TCAAACGTGT TAGCGGCACA ATCCCATACA CGGCTCCAGA ACTCTGCGAT 
ACGTCCAAAC ACGACGGCTT CTGCGTGGAC TACAGCACCG ATGTGTGGGC GTTCGGAGTG 
CTGCTGTTCT GCATGCTAAC CGGGAACTTT CCATGGGAAA AAGCGATGCC GTCTGATACT 
TTCTACGAGG AGTTCGTGCG CTGGCAGAAG CGGCGGACGG GTGCGGTTCC GTCACAATGG 
CGTCGATTTA CAGACGAATC CCTGCGCATG TTTCGAAAGC TCCTAGCACT GGAGCAGGAA 
CGCAGATGCA GCGTCAAGGA GGTTTTCGCA CATTTAGGCC ACCGCTGGAT GCTTGATGGA 
ACTAGCGGAA ACCATCATCA ATCCGTGCTC AACTCTTCAT CGGAGGAAGA CGAGTTGCTT 
GTGGATCGTA TGAAGCAGCA GACCTTATCT CCGACAGCTA ACACGAGCAA TGCTATTGAG 
CCCGGATCGG CTAACCACTT CACATCTATG TCCACCAATA GCTCGGTGTC TTCCACTAAC 
AGCTATGAGC GTTCGGCTAG AGACAGTCCT CCGACTAGCA GGATCCTGGT CACGACGCCT 
ATAGAAATCT GCGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bsk146 ( XM_009306356.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304631.1 Danio rerio brain specific kinase 146 (bsk146), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa33030
Clone ID Related Accession (Same CDS sequence)	XM_009306356.2 , XM_009306356.3 , NM_212841.2
Accession Version	XM_009306356.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase SBK1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009306356.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCGT CTCCGGTGGT TTCCCATGAC ATTCTGGAGG AGCTGCAGCT CTACACCGCT 
CAGAACCTGG AGAAGCTGGA GGTCAATAAA TACTATGAGG TGATCCGGGA GCTCGGGAAG 
GGCACTTACG GGAAGGTGGA CCTCGTCATT CATAAGATCA GAGGTAGCAA AATGGCTCTG 
AAGTTTCTGA AGAAGAAAAG CACCAAGCTG AAGAGCTTCT TGCGGGAGTA CAGCATCTCT 
CTCTATCTCT CTCCATGTCC CTTCATCATC AACATGTTCG GCATCGCCTT TGAGACAGAC 
GAGTATTATG TGTTTGCGCA GGAATACGCT CCTTCTGGGG ACCTGTTTGA CATCATTCCA 
CCACAGGTGG GTCTGCCAGA GCCGGTGGCG AAGCGCTGTG TGCATCAGGT GGCCATCGCT 
CTCGAATACC TGCACTCCAA AAAACTGGTG CACCGAGACA TCAAACCCGA GAACATCCTA 
ATCTTCGACA AGGAGTGCCG TAAGGTCAAG CTGTCAGACT TCGGGATGGC GCGGCGCGCC 
GGTTCACCGG TCAAGCGTGT TAGCGGCACA ATCCCATACA CGGCTCCAGA ACTCTGCGAT 
ACGTCCAAAC ACGACGGCTT CTGCGTGGAC TACAGCACCG ATGTGTGGGC GTTCGGAGTG 
CTGCTGTTCT GCATGCTAAC CGGGAACTTT CCATGGGAAA AAGCGATGCC GTCTGATACT 
TTCTACGAGG AGTTTGTGCG CTGGCAGAAG CGGCGGACGG GTGCGGTTCC GTCACAATGG 
CGTCGATTTA CAGACGAATC CCTGCGCATG TTTCGAAAGC TCCTAGCATT GGAGCAGGAA 
CGCAGATGCA GCGTCAAGGA GGTTTTCGCA CATTTAGGCC ACCGCTGGAT GCTTGATGGA 
ACTAGCGGAA ACCATCATCA ATCCGTGCTC AACTCTTCAT CAGAGGAAGA CGAATTGCTT 
GTGGATCGTA TGAAGCAGCA GACCTTATCT CCGACAGCTA ACACGAGCAA TGCTATTGAG 
CCCGGATCGG CTAACCACTT CACATCTGTG TCCACCAATA GCTCGGTGTC TTCCACTAAC 
AGCTATGAGC GTTCGGCTAG AGACAGTCCT CCGACTAGCA GGATCCTGGT CACGACGCCT 
ATAGAAATCT GCGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304631.1
CDS	399..1556
Translation	MSSSPVVSHDILEELQLYTAQNLEKLEVNKYYEVIRELGKGTYGKVDLVIHKIRGSKMALKFLKKKSTKLKSFLREYSISLYLSPCPFIINMFGIAFETDEYYVFAQEYAPSGDLFDIIPPQVGLPEPVAKRCVHQVAIALEYLHSKKLVHRDIKPENILIFDKECRKVKLSDFGMARRAGSPVKRVSGTIPYTAPELCDTSKHDGFCVDYSTDVWAFGVLLFCMLTGNFPWEKAMPSDTFYEEFVRWQKRRTGAVPSQWRRFTDESLRMFRKLLALEQERRCSVKEVFAHLGHRWMLDGTSGNHHQSVLNSSSEEDELLVDRMKQQTLSPTANTSNAIEPGSANHFTSVSTNSSVSSTNSYERSARDSPPTSRILVTTPIEICV

Target ORF information:

RefSeq Version	XM_009306356.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio brain specific kinase 146 (bsk146), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306356.2

ORF Insert Sequence:

ATGAGCTCGT CTCCGGTGGT TTCCCATGAC ATTCTGGAGG AGCTGCAGCT CTACACCGCT 
CAGAACCTGG AGAAGCTGGA GGTCAATAAA TACTATGAGG TGATCCGGGA GCTCGGGAAG 
GGCACTTACG GGAAGGTGGA CCTCGTCATT CATAAGATCA GAGGTAGCAA AATGGCTCTG 
AAGTTTCTGA AGAAGAAAAG CACCAAGCTG AAGAGCTTCT TGCGGGAGTA CAGCATCTCT 
CTCTATCTCT CTCCATGTCC CTTCATCATC AACATGTTCG GCATCGCCTT TGAGACAGAC 
GAGTATTATG TGTTTGCGCA GGAATACGCT CCTTCTGGGG ACCTGTTTGA CATCATTCCA 
CCACAGGTGG GTCTGCCAGA GCCGGTGGCG AAGCGCTGTG TGCATCAGGT GGCCATCGCT 
CTCGAATACC TGCACTCCAA AAAACTGGTG CACCGAGACA TCAAACCCGA GAACATCCTA 
ATCTTCGACA AGGAGTGCCG TAAGGTCAAG CTGTCAGACT TCGGGATGGC GCGGCGCGCC 
GGTTCACCGG TCAAGCGTGT TAGCGGCACA ATCCCATACA CGGCTCCAGA ACTCTGCGAT 
ACGTCCAAAC ACGACGGCTT CTGCGTGGAC TACAGCACCG ATGTGTGGGC GTTCGGAGTG 
CTGCTGTTCT GCATGCTAAC CGGGAACTTT CCATGGGAAA AAGCGATGCC GTCTGATACT 
TTCTACGAGG AGTTTGTGCG CTGGCAGAAG CGGCGGACGG GTGCGGTTCC GTCACAATGG 
CGTCGATTTA CAGACGAATC CCTGCGCATG TTTCGAAAGC TCCTAGCATT GGAGCAGGAA 
CGCAGATGCA GCGTCAAGGA GGTTTTCGCA CATTTAGGCC ACCGCTGGAT GCTTGATGGA 
ACTAGCGGAA ACCATCATCA ATCCGTGCTC AACTCTTCAT CAGAGGAAGA CGAATTGCTT 
GTGGATCGTA TGAAGCAGCA GACCTTATCT CCGACAGCTA ACACGAGCAA TGCTATTGAG 
CCCGGATCGG CTAACCACTT CACATCTGTG TCCACCAATA GCTCGGTGTC TTCCACTAAC 
AGCTATGAGC GTTCGGCTAG AGACAGTCCT CCGACTAGCA GGATCCTGGT CACGACGCCT 
ATAGAAATCT GCGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

bsk146 ( XM_009306356.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009304631.1 Danio rerio brain specific kinase 146 (bsk146), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa33030
Clone ID Related Accession (Same CDS sequence)	XM_009306356.2 , XM_009306356.3 , NM_212841.2
Accession Version	XM_009306356.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1158bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase SBK1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007123.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009306356.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAGCTCGT CTCCGGTGGT TTCCCATGAC ATTCTGGAGG AGCTGCAGCT CTACACCGCT 
CAGAACCTGG AGAAGCTGGA GGTCAATAAA TACTATGAGG TGATCCGGGA GCTCGGGAAG 
GGCACTTACG GGAAGGTGGA CCTCGTCATT CATAAGATCA GAGGTAGCAA AATGGCTCTG 
AAGTTTCTGA AGAAGAAAAG CACCAAGCTG AAGAGCTTCT TGCGGGAGTA CAGCATCTCT 
CTCTATCTCT CTCCATGTCC CTTCATCATC AACATGTTCG GCATCGCCTT TGAGACAGAC 
GAGTATTATG TGTTTGCGCA GGAATACGCT CCTTCTGGGG ACCTGTTTGA CATCATTCCA 
CCACAGGTGG GTCTGCCAGA GCCGGTGGCG AAGCGCTGTG TGCATCAGGT GGCCATCGCT 
CTCGAATACC TGCACTCCAA AAAACTGGTG CACCGAGACA TCAAACCCGA GAACATCCTA 
ATCTTCGACA AGGAGTGCCG TAAGGTCAAG CTGTCAGACT TCGGGATGGC GCGGCGCGCC 
GGTTCACCGG TCAAGCGTGT TAGCGGCACA ATCCCATACA CGGCTCCAGA ACTCTGCGAT 
ACGTCCAAAC ACGACGGCTT CTGCGTGGAC TACAGCACCG ATGTGTGGGC GTTCGGAGTG 
CTGCTGTTCT GCATGCTAAC CGGGAACTTT CCATGGGAAA AAGCGATGCC GTCTGATACT 
TTCTACGAGG AGTTTGTGCG CTGGCAGAAG CGGCGGACGG GTGCGGTTCC GTCACAATGG 
CGTCGATTTA CAGACGAATC CCTGCGCATG TTTCGAAAGC TCCTAGCATT GGAGCAGGAA 
CGCAGATGCA GCGTCAAGGA GGTTTTCGCA CATTTAGGCC ACCGCTGGAT GCTTGATGGA 
ACTAGCGGAA ACCATCATCA ATCCGTGCTC AACTCTTCAT CAGAGGAAGA CGAATTGCTT 
GTGGATCGTA TGAAGCAGCA GACCTTATCT CCGACAGCTA ACACGAGCAA TGCTATTGAG 
CCCGGATCGG CTAACCACTT CACATCTGTG TCCACCAATA GCTCGGTGTC TTCCACTAAC 
AGCTATGAGC GTTCGGCTAG AGACAGTCCT CCGACTAGCA GGATCCTGGT CACGACGCCT 
ATAGAAATCT GCGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009304631.1
CDS	246..1403
Translation	MSSSPVVSHDILEELQLYTAQNLEKLEVNKYYEVIRELGKGTYGKVDLVIHKIRGSKMALKFLKKKSTKLKSFLREYSISLYLSPCPFIINMFGIAFETDEYYVFAQEYAPSGDLFDIIPPQVGLPEPVAKRCVHQVAIALEYLHSKKLVHRDIKPENILIFDKECRKVKLSDFGMARRAGSPVKRVSGTIPYTAPELCDTSKHDGFCVDYSTDVWAFGVLLFCMLTGNFPWEKAMPSDTFYEEFVRWQKRRTGAVPSQWRRFTDESLRMFRKLLALEQERRCSVKEVFAHLGHRWMLDGTSGNHHQSVLNSSSEEDELLVDRMKQQTLSPTANTSNAIEPGSANHFTSVSTNSSVSSTNSYERSARDSPPTSRILVTTPIEICV

Target ORF information:

RefSeq Version	XM_009306356.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio brain specific kinase 146 (bsk146), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009306356.3

ORF Insert Sequence:

ATGAGCTCGT CTCCGGTGGT TTCCCATGAC ATTCTGGAGG AGCTGCAGCT CTACACCGCT 
CAGAACCTGG AGAAGCTGGA GGTCAATAAA TACTATGAGG TGATCCGGGA GCTCGGGAAG 
GGCACTTACG GGAAGGTGGA CCTCGTCATT CATAAGATCA GAGGTAGCAA AATGGCTCTG 
AAGTTTCTGA AGAAGAAAAG CACCAAGCTG AAGAGCTTCT TGCGGGAGTA CAGCATCTCT 
CTCTATCTCT CTCCATGTCC CTTCATCATC AACATGTTCG GCATCGCCTT TGAGACAGAC 
GAGTATTATG TGTTTGCGCA GGAATACGCT CCTTCTGGGG ACCTGTTTGA CATCATTCCA 
CCACAGGTGG GTCTGCCAGA GCCGGTGGCG AAGCGCTGTG TGCATCAGGT GGCCATCGCT 
CTCGAATACC TGCACTCCAA AAAACTGGTG CACCGAGACA TCAAACCCGA GAACATCCTA 
ATCTTCGACA AGGAGTGCCG TAAGGTCAAG CTGTCAGACT TCGGGATGGC GCGGCGCGCC 
GGTTCACCGG TCAAGCGTGT TAGCGGCACA ATCCCATACA CGGCTCCAGA ACTCTGCGAT 
ACGTCCAAAC ACGACGGCTT CTGCGTGGAC TACAGCACCG ATGTGTGGGC GTTCGGAGTG 
CTGCTGTTCT GCATGCTAAC CGGGAACTTT CCATGGGAAA AAGCGATGCC GTCTGATACT 
TTCTACGAGG AGTTTGTGCG CTGGCAGAAG CGGCGGACGG GTGCGGTTCC GTCACAATGG 
CGTCGATTTA CAGACGAATC CCTGCGCATG TTTCGAAAGC TCCTAGCATT GGAGCAGGAA 
CGCAGATGCA GCGTCAAGGA GGTTTTCGCA CATTTAGGCC ACCGCTGGAT GCTTGATGGA 
ACTAGCGGAA ACCATCATCA ATCCGTGCTC AACTCTTCAT CAGAGGAAGA CGAATTGCTT 
GTGGATCGTA TGAAGCAGCA GACCTTATCT CCGACAGCTA ACACGAGCAA TGCTATTGAG 
CCCGGATCGG CTAACCACTT CACATCTGTG TCCACCAATA GCTCGGTGTC TTCCACTAAC 
AGCTATGAGC GTTCGGCTAG AGACAGTCCT CCGACTAGCA GGATCCTGGT CACGACGCCT 
ATAGAAATCT GCGTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Identification of single-nucleotide polymorphism markers associated with cortisol response to crowding in rainbow trout.
Marine biotechnology (New York, N.Y.)17(3)328-37(2015 Jun)
Liu S,Vallejo RL,Gao G,Palti Y,Weber GM,Hernandez A,Rexroad CE 3rd

Gene expression profiling in zebrafish embryos exposed to diclofenac, an environmental toxicant.
Molecular biology reports39(3)2119-28(2012 Mar)
De Felice B,Copia L,Guida M

Expression and characterization of a brain-specific protein kinase BSK146 from zebrafish.
Biochemical and biophysical research communications340(3)767-75(2006 Feb)
Chou CM,Chen YC,Lee MT,Chen GD,Lu IC,Chen ST,Huang CJ

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bsk146 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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