chad cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	chad
Entrez Gene ID	394038
Full Name	chondroadherin
Synonyms	zgc:63475
General protein information	Preferred Names chondroadherin Names chondroadherin
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_201063.1	NP_957357.1	chondroadherin precursor

Pathways from BioSystems

Homologies

Canis lupus familiaris (dog)	CHAD	XP_852404.2
Xenopus tropicalis (tropical clawed frog)	chad	XP_004918539.1
Bos taurus (cattle)	CHAD	NP_776444.1
Pan troglodytes (chimpanzee)	CHAD	XP_001170009.2
	LOC702601	XP_001098094.2
Mus musculus (house mouse)	Chad	NP_031715.1
Rattus norvegicus (Norway rat)	Chad	NP_062037.1
Homo sapiens (human)	CHAD	NP_001258.2
Gallus gallus (chicken)	CHAD	XP_004946392.1
Danio rerio (zebrafish)	chad	NP_957357.1

Gene Ontology
Bibliography

Related articles in PubMed

Zebrafish Collagen Type I: Molecular and Biochemical Characterization of the Major Structural Protein in Bone and Skin.
Gistelinck C, Gioia R, Gagliardi A, Tonelli F, Marchese L, Bianchi L, Landi C, Bini L, Huysseune A, Witten PE, Staes A, Gevaert K, De Rocker N, Menten B, Malfait F, Leikin S, Carra S, Tenni R, Rossi A, De Paepe A, Coucke P, Willaert A, Forlino A
Scientific reports621540(2016 Feb)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Comparative transcriptome analyses indicate molecular homology of zebrafish swimbladder and mammalian lung.
Zheng W, Wang Z, Collins JE, Andrews RM, Stemple D, Gong Z
PloS one6(8)e24019(2011)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following chad gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the chad cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa32749	NM_201063.1 Latest version!	Danio rerio chondroadherin (chad), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$258.30 $369.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

chad ( NM_201063.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_957357.1 Danio rerio chondroadherin (chad), mRNA.

Price & Availability↑

CloneID	ODa32749
Clone ID Related Accession (Same CDS sequence)	NM_201063.1
Accession Version	NM_201063.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 1092bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-10-05
Organism	Danio rerio(zebrafish)
Product	chondroadherin precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC054898.1. ##Evidence-Data-START## Transcript exon combination :: BC054898.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTACACTT TTCATGTCTT ACTGGTGCTA GTGTGTCTAC AGTTTTGTGC TCACACTGCT 
TTTGCAGCTC CAACTCAGTG CCCAAGCCAG TGCCACTGCC ATGGGGACCT GCAGCATGTT 
ATTTGTGACA ATGTTGGGCT AAAGAAGATC CCTCGCATAT CTGAAGCCAC GCGTCTCCTC 
AACCTGCAAC GCAACAACTT GGGGAATCTA CCAACTGGGG GCTTCAGTGA GATGAAAGGG 
CTCATTTCTC TGCACCTGCA GCATTGCCAG ATCCGAGAGC TCTCCGGCCA GGCTTTCAAA 
GGGCTCAACA AGCTCATCTA CCTCTACCTG TCTGATAATG AGATCAGCAC CATTAAACCC 
GGTGCTTTTG AGGATCTAAC AGAACTCACC TATCTCTACT TGGACGGCAA CCAAATCACA 
AGCCTTCCGA AGGGCATCTT TTCCCCCATG ATTAACCTGT TCATCCTGCA GCTCAACAAC 
AACAAGCTTC GAGAGCTGCA GCCTGGTACT TTCAAAGGTG CTAAGGATCT CCGCTGGCTT 
TACATGAGTG GCAACGAGCT GAGCTCCATA CAGCCTGGTT CTCTTGATGA AGTGGAGAAC 
TTGGCCATTC TAACCCTGGA CCAGAACAAC CTGTCCACAT ACCCTCTCCT GGCTATGAGC 
AAGCTGCGTG TTGTGGAGGA ACTCAACCTC TCCAAAAACC CTCTCAGCCT CATCCCTGAC 
CATGCATTCC GAAGCTTCGG ACGCTACATG GAGAAGCTCC ATCTAAATGA CATGAGCCTG 
GAGAAGTTTT CCAATGCTGC CTTTGAGGGA GTGACGGCTC TCAAATCTCT GCATCTGGAG 
AACAACAAGC TGAGGTCACT ACCAAACAGC CTTGAGTTCA GCACCATCCA AAACATCACT 
CTGTTTAACA ATCCCTGGAG CTGCACCTGC CCATTAACAA ACCTCAGAAA ATGGATGGAC 
ACTACTCGTA ATCGCCCAGA TGCAGTTTGC GCTTCACCTG CCAACCAGAA AGGAAAGCAA 
ATAAGAGACA GCACTGCTTT CACCCGCTGC AAGGGAAAAA CAAAGAGGGC CAGGAAGGGG 
ACACGACTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_957357.1
CDS	74..1165
Translation	MYTFHVLLVLVCLQFCAHTAFAAPTQCPSQCHCHGDLQHVICDNVGLKKIPRISEATRLLNLQRNNLGNLPTGGFSEMKGLISLHLQHCQIRELSGQAFKGLNKLIYLYLSDNEISTIKPGAFEDLTELTYLYLDGNQITSLPKGIFSPMINLFILQLNNNKLRELQPGTFKGAKDLRWLYMSGNELSSIQPGSLDEVENLAILTLDQNNLSTYPLLAMSKLRVVEELNLSKNPLSLIPDHAFRSFGRYMEKLHLNDMSLEKFSNAAFEGVTALKSLHLENNKLRSLPNSLEFSTIQNITLFNNPWSCTCPLTNLRKWMDTTRNRPDAVCASPANQKGKQIRDSTAFTRCKGKTKRARKGTRL

Target ORF information:

RefSeq Version	NM_201063.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio chondroadherin (chad), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_201063.1

ORF Insert Sequence:

ATGTACACTT TTCATGTCTT ACTGGTGCTA GTGTGTCTAC AGTTTTGTGC TCACACTGCT 
TTTGCAGCTC CAACTCAGTG CCCAAGCCAG TGCCACTGCC ATGGGGACCT GCAGCATGTT 
ATTTGTGACA ATGTTGGGCT AAAGAAGATC CCTCGCATAT CTGAAGCCAC GCGTCTCCTC 
AACCTGCAAC GCAACAACTT GGGGAATCTA CCAACTGGGG GCTTCAGTGA GATGAAAGGG 
CTCATTTCTC TGCACCTGCA GCATTGCCAG ATCCGAGAGC TCTCCGGCCA GGCTTTCAAA 
GGGCTCAACA AGCTCATCTA CCTCTACCTG TCTGATAATG AGATCAGCAC CATTAAACCC 
GGTGCTTTTG AGGATCTAAC AGAACTCACC TATCTCTACT TGGACGGCAA CCAAATCACA 
AGCCTTCCGA AGGGCATCTT TTCCCCCATG ATTAACCTGT TCATCCTGCA GCTCAACAAC 
AACAAGCTTC GAGAGCTGCA GCCTGGTACT TTCAAAGGTG CTAAGGATCT CCGCTGGCTT 
TACATGAGTG GCAACGAGCT GAGCTCCATA CAGCCTGGTT CTCTTGATGA AGTGGAGAAC 
TTGGCCATTC TAACCCTGGA CCAGAACAAC CTGTCCACAT ACCCTCTCCT GGCTATGAGC 
AAGCTGCGTG TTGTGGAGGA ACTCAACCTC TCCAAAAACC CTCTCAGCCT CATCCCTGAC 
CATGCATTCC GAAGCTTCGG ACGCTACATG GAGAAGCTCC ATCTAAATGA CATGAGCCTG 
GAGAAGTTTT CCAATGCTGC CTTTGAGGGA GTGACGGCTC TCAAATCTCT GCATCTGGAG 
AACAACAAGC TGAGGTCACT ACCAAACAGC CTTGAGTTCA GCACCATCCA AAACATCACT 
CTGTTTAACA ATCCCTGGAG CTGCACCTGC CCATTAACAA ACCTCAGAAA ATGGATGGAC 
ACTACTCGTA ATCGCCCAGA TGCAGTTTGC GCTTCACCTG CCAACCAGAA AGGAAAGCAA 
ATAAGAGACA GCACTGCTTT CACCCGCTGC AAGGGAAAAA CAAAGAGGGC CAGGAAGGGG 
ACACGACTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Zebrafish Collagen Type I: Molecular and Biochemical Characterization of the Major Structural Protein in Bone and Skin.
Scientific reports621540(2016 Feb)
Gistelinck C,Gioia R,Gagliardi A,Tonelli F,Marchese L,Bianchi L,Landi C,Bini L,Huysseune A,Witten PE,Staes A,Gevaert K,De Rocker N,Menten B,Malfait F,Leikin S,Carra S,Tenni R,Rossi A,De Paepe A,Coucke P,Willaert A,Forlino A

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Comparative transcriptome analyses indicate molecular homology of zebrafish swimbladder and mammalian lung.
PloS one6(8)e24019(2011)
Zheng W,Wang Z,Collins JE,Andrews RM,Stemple D,Gong Z

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chad cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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