pdcd10a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	pdcd10a
Entrez Gene ID	393527
Full Name	programmed cell death 10a
Synonyms	ccm3a,pdcd10,zgc:65826
General protein information	Preferred Names programmed cell death 10a Names programmed cell death protein 10-A programmed cell death 10
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_200555.2	NP_956849.2	programmed cell death protein 10-A
XM_005158980.3	XP_005159037.1	programmed cell death protein 10-A isoform X1
XM_005158980.4	XP_005159037.1	programmed cell death protein 10-A isoform X1

Pathways from BioSystems

Homologies

Homo sapiens (human)	PDCD10	NP_665858.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008287	XP_001689007.1
Canis lupus familiaris (dog)	PDCD10	XP_545269.1
Pan troglodytes (chimpanzee)	PDCD10	XP_001160479.1
Danio rerio (zebrafish)	pdcd10a	NP_956849.2
Bos taurus (cattle)	PDCD10	NP_001068727.1
Mus musculus (house mouse)	Pdcd10	NP_062719.2
Rattus norvegicus (Norway rat)	Pdcd10	NP_001009542.1
Xenopus tropicalis (tropical clawed frog)	pdcd10	NP_001006850.1
	PDCD10	XP_002802977.1
Gallus gallus (chicken)	PDCD10	NP_001006554.1
Drosophila melanogaster (fruit fly)	CG5073	NP_650459.1
Caenorhabditis elegans (roundworm)	C14A4.11	NP_496290.2

Gene Ontology
Bibliography

Related articles in PubMed

Evolution of complexity in the zebrafish synapse proteome.
Bay?s ?, Collins MO, Reig-Viader R, Gou G, Goulding D, Izquierdo A, Choudhary JS, Emes RD, Grant SG
Nature communications814613(2017 Mar)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

First description of programmed cell death10 (PDCD10) in rock bream (Oplegnathus fasciatus): Potential relations to the regulation of apoptosis by several pathogens.
Kim JW, Jeong JM, Bae JS, Cho DH, Jung SH, Hwang JY, Kwon MG, Seo JS, Baeck GW, Park CI
Developmental and comparative immunology5551-5(2016 Feb)

Ccm3 functions in a manner distinct from Ccm1 and Ccm2 in a zebrafish model of CCM vascular disease.
Yoruk B, Gillers BS, Chi NC, Scott IC
Developmental biology362(2)121-31(2012 Feb)

CCM3/PDCD10 heterodimerizes with germinal center kinase III (GCKIII) proteins using a mechanism analogous to CCM3 homodimerization.
Ceccarelli DF, Laister RC, Mulligan VK, Kean MJ, Goudreault M, Scott IC, Derry WB, Chakrabartty A, Gingras AC, Sicheri F
The Journal of biological chemistry286(28)25056-64(2011 Jul)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

CCM3 plays a role distinct from CCM1/2 in Cerebral cavernous malformations pathogenesis, and acts via GCKIII activity to regulate cranial vasculature integrity and development.
Title: Ccm3 functions in a manner distinct from Ccm1 and Ccm2 in a zebrafish model of CCM vascular disease.

Sequence conservation and binding studies suggest that CCM3 may preferentially heterodimerize with GCKIII proteins through a manner structurally analogous to that employed for CCM3 homodimerization.
Title: CCM3/PDCD10 heterodimerizes with germinal center kinase III (GCKIII) proteins using a mechanism analogous to CCM3 homodimerization.

The newly mapped STK25 and MST4 interaction domain within the CCM3 protein plays a crucial role for vascular development in zebrafish.
Title: Functional analyses of human and zebrafish 18-amino acid in-frame deletion pave the way for domain mapping of the cerebral cavernous malformation 3 protein.

The following pdcd10a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the pdcd10a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa23034	NM_200555.2 Latest version!	Danio rerio programmed cell death 10a (pdcd10a), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
ODa23034	XM_005158980.4 Latest version!	Danio rerio programmed cell death 10a (pdcd10a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
View Old Accession Versions >>
ODa23034	XM_005158980.3	Danio rerio programmed cell death 10a (pdcd10a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$174.30 $249.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

pdcd10a ( NM_200555.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956849.2 Danio rerio programmed cell death 10a (pdcd10a), mRNA.

Price & Availability↑

CloneID	ODa23034
Clone ID Related Accession (Same CDS sequence)	NM_200555.2 , XM_005158980.3 , XM_005158980.4
Accession Version	NM_200555.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-01-26
Organism	Danio rerio(zebrafish)
Product	programmed cell death protein 10-A
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from EH548629.1, CABZ01071280.1 and CABZ01071282.1. On Sep 30, 2011 this sequence version replaced NM_200555.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: BC056552.1, EH590864.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGACAATGG AAGAGATGAA GAATGAAGCG GACGCGACCT CCATGGTATC CATGACCCTC 
TACGCCGTGA TGTACCCGGT GTTCAATGAG CTGGAGAGTG TAAATCTGTC TGCGGCACAG 
ACTCTGCGAG CTGCTTTCAA AAAGGCAGAG AAGGAAAACC CAGGACTGAC TCAGGACATT 
ATCATGAAAA TACTGGAGAA GAAGAACGTG GAGATCAACT TCACCGAGTC TTTATTACGC 
ATGGCTGCTG ATGATGTTGA GGAGTACATG ATTGACAGGC CCGAGCGGGA GTTCCAGGAC 
CTGAATGAAA GAGCTCGCGC GCTGAAACAG ATCCTCAGCA AAATCCCAGA CGAAATTAAT 
GACCGAGTCC GATTCCTGCA GACAATCAAA GATATAGCCA GTGCCATAAA AGAGCTGCTG 
GACACAGTGA ACAACGTCTT CAAGAAATAT CAGTACCAGA ACAGAAGGGC ACTTGAGCAT 
CAGAAGAAAG AGTTTGTGAA ACATTCGAAA AGCTTCAGCG ACACTCTGAA AACATACTTC 
AAAGACGGAA AGGCAATAAA CGTGTTTGCC AGTGCCAACC GGCTCATCCA CCAAACCAAC 
TTGATACTGC AGACCTTCAA AACTGTCGCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956849.2
CDS	180..812
Translation	MTMEEMKNEADATSMVSMTLYAVMYPVFNELESVNLSAAQTLRAAFKKAEKENPGLTQDIIMKILEKKNVEINFTESLLRMAADDVEEYMIDRPEREFQDLNERARALKQILSKIPDEINDRVRFLQTIKDIASAIKELLDTVNNVFKKYQYQNRRALEHQKKEFVKHSKSFSDTLKTYFKDGKAINVFASANRLIHQTNLILQTFKTVA

Target ORF information:

RefSeq Version	NM_200555.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio programmed cell death 10a (pdcd10a), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200555.2

ORF Insert Sequence:

ATGACAATGG AAGAGATGAA GAATGAAGCG GACGCGACCT CCATGGTATC CATGACCCTC 
TACGCCGTGA TGTACCCGGT GTTCAATGAG CTGGAGAGTG TAAATCTGTC TGCGGCACAG 
ACTCTGCGAG CTGCTTTCAA AAAGGCAGAG AAGGAAAACC CAGGACTGAC TCAGGACATT 
ATCATGAAAA TACTGGAGAA GAAGAACGTG GAGATCAACT TCACCGAGTC TTTATTACGC 
ATGGCTGCTG ATGATGTTGA GGAGTACATG ATTGACAGGC CCGAGCGGGA GTTCCAGGAC 
CTGAATGAAA GAGCTCGCGC GCTGAAACAG ATCCTCAGCA AAATCCCAGA CGAAATTAAT 
GACCGAGTCC GATTCCTGCA GACAATCAAA GATATAGCCA GTGCCATAAA AGAGCTGCTG 
GACACAGTGA ACAACGTCTT CAAGAAATAT CAGTACCAGA ACAGAAGGGC ACTTGAGCAT 
CAGAAGAAAG AGTTTGTGAA ACATTCGAAA AGCTTCAGCG ACACTCTGAA AACATACTTC 
AAAGACGGAA AGGCAATAAA CGTGTTTGCC AGTGCCAACC GGCTCATCCA CCAAACCAAC 
TTGATACTGC AGACCTTCAA AACTGTCGCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pdcd10a ( XM_005158980.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159037.1 Danio rerio programmed cell death 10a (pdcd10a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa23034
Clone ID Related Accession (Same CDS sequence)	NM_200555.2 , XM_005158980.3 , XM_005158980.4
Accession Version	XM_005158980.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	programmed cell death protein 10-A isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005158980.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAATGG AAGAGATGAA GAATGAAGCG GACGCGACCT CCATGGTATC CATGACCCTC 
TACGCCGTGA TGTACCCGGT GTTCAATGAG CTGGAGAGTG TAAATCTGTC TGCGGCACAG 
ACTCTGCGAG CTGCTTTCAA AAAGGCAGAG AAGGAAAACC CAGGACTGAC TCAGGACATT 
ATCATGAAAA TACTGGAGAA GAAGAACGTG GAGATCAACT TCACCGAGTC TTTATTACGC 
ATGGCTGCTG ATGATGTTGA GGAGTACATG ATTGACAGGC CCGAGCGGGA GTTCCAGGAC 
CTGAATGAAA GAGCTCGCGC GCTGAAACAG ATCCTCAGCA AAATCCCAGA CGAAATTAAT 
GACCGAGTCC GATTCCTGCA GACAATCAAA GATATAGCCA GTGCCATAAA AGAGCTGCTG 
GACACAGTGA ACAACGTCTT CAAGAAATAT CAGTACCAGA ACAGAAGGGC ACTTGAGCAT 
CAGAAGAAAG AGTTTGTGAA ACATTCGAAA AGCTTCAGCG ACACTCTGAA AACATACTTC 
AAAGACGGAA AGGCAATAAA CGTGTTTGCC AGTGCCAACC GGCTCATCCA CCAAACCAAC 
TTGATACTGC AGACCTTCAA AACTGTCGCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159037.1
CDS	153..785
Translation	MTMEEMKNEADATSMVSMTLYAVMYPVFNELESVNLSAAQTLRAAFKKAEKENPGLTQDIIMKILEKKNVEINFTESLLRMAADDVEEYMIDRPEREFQDLNERARALKQILSKIPDEINDRVRFLQTIKDIASAIKELLDTVNNVFKKYQYQNRRALEHQKKEFVKHSKSFSDTLKTYFKDGKAINVFASANRLIHQTNLILQTFKTVA

Target ORF information:

RefSeq Version	XM_005158980.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio programmed cell death 10a (pdcd10a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158980.3

ORF Insert Sequence:

ATGACAATGG AAGAGATGAA GAATGAAGCG GACGCGACCT CCATGGTATC CATGACCCTC 
TACGCCGTGA TGTACCCGGT GTTCAATGAG CTGGAGAGTG TAAATCTGTC TGCGGCACAG 
ACTCTGCGAG CTGCTTTCAA AAAGGCAGAG AAGGAAAACC CAGGACTGAC TCAGGACATT 
ATCATGAAAA TACTGGAGAA GAAGAACGTG GAGATCAACT TCACCGAGTC TTTATTACGC 
ATGGCTGCTG ATGATGTTGA GGAGTACATG ATTGACAGGC CCGAGCGGGA GTTCCAGGAC 
CTGAATGAAA GAGCTCGCGC GCTGAAACAG ATCCTCAGCA AAATCCCAGA CGAAATTAAT 
GACCGAGTCC GATTCCTGCA GACAATCAAA GATATAGCCA GTGCCATAAA AGAGCTGCTG 
GACACAGTGA ACAACGTCTT CAAGAAATAT CAGTACCAGA ACAGAAGGGC ACTTGAGCAT 
CAGAAGAAAG AGTTTGTGAA ACATTCGAAA AGCTTCAGCG ACACTCTGAA AACATACTTC 
AAAGACGGAA AGGCAATAAA CGTGTTTGCC AGTGCCAACC GGCTCATCCA CCAAACCAAC 
TTGATACTGC AGACCTTCAA AACTGTCGCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

pdcd10a ( XM_005158980.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005159037.1 Danio rerio programmed cell death 10a (pdcd10a), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa23034
Clone ID Related Accession (Same CDS sequence)	NM_200555.2 , XM_005158980.3 , XM_005158980.4
Accession Version	XM_005158980.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 633bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	programmed cell death protein 10-A isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005158980.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGACAATGG AAGAGATGAA GAATGAAGCG GACGCGACCT CCATGGTATC CATGACCCTC 
TACGCCGTGA TGTACCCGGT GTTCAATGAG CTGGAGAGTG TAAATCTGTC TGCGGCACAG 
ACTCTGCGAG CTGCTTTCAA AAAGGCAGAG AAGGAAAACC CAGGACTGAC TCAGGACATT 
ATCATGAAAA TACTGGAGAA GAAGAACGTG GAGATCAACT TCACCGAGTC TTTATTACGC 
ATGGCTGCTG ATGATGTTGA GGAGTACATG ATTGACAGGC CCGAGCGGGA GTTCCAGGAC 
CTGAATGAAA GAGCTCGCGC GCTGAAACAG ATCCTCAGCA AAATCCCAGA CGAAATTAAT 
GACCGAGTCC GATTCCTGCA GACAATCAAA GATATAGCCA GTGCCATAAA AGAGCTGCTG 
GACACAGTGA ACAACGTCTT CAAGAAATAT CAGTACCAGA ACAGAAGGGC ACTTGAGCAT 
CAGAAGAAAG AGTTTGTGAA ACATTCGAAA AGCTTCAGCG ACACTCTGAA AACATACTTC 
AAAGACGGAA AGGCAATAAA CGTGTTTGCC AGTGCCAACC GGCTCATCCA CCAAACCAAC 
TTGATACTGC AGACCTTCAA AACTGTCGCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005159037.1
CDS	129..761
Translation	MTMEEMKNEADATSMVSMTLYAVMYPVFNELESVNLSAAQTLRAAFKKAEKENPGLTQDIIMKILEKKNVEINFTESLLRMAADDVEEYMIDRPEREFQDLNERARALKQILSKIPDEINDRVRFLQTIKDIASAIKELLDTVNNVFKKYQYQNRRALEHQKKEFVKHSKSFSDTLKTYFKDGKAINVFASANRLIHQTNLILQTFKTVA

Target ORF information:

RefSeq Version	XM_005158980.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio programmed cell death 10a (pdcd10a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005158980.4

ORF Insert Sequence:

ATGACAATGG AAGAGATGAA GAATGAAGCG GACGCGACCT CCATGGTATC CATGACCCTC 
TACGCCGTGA TGTACCCGGT GTTCAATGAG CTGGAGAGTG TAAATCTGTC TGCGGCACAG 
ACTCTGCGAG CTGCTTTCAA AAAGGCAGAG AAGGAAAACC CAGGACTGAC TCAGGACATT 
ATCATGAAAA TACTGGAGAA GAAGAACGTG GAGATCAACT TCACCGAGTC TTTATTACGC 
ATGGCTGCTG ATGATGTTGA GGAGTACATG ATTGACAGGC CCGAGCGGGA GTTCCAGGAC 
CTGAATGAAA GAGCTCGCGC GCTGAAACAG ATCCTCAGCA AAATCCCAGA CGAAATTAAT 
GACCGAGTCC GATTCCTGCA GACAATCAAA GATATAGCCA GTGCCATAAA AGAGCTGCTG 
GACACAGTGA ACAACGTCTT CAAGAAATAT CAGTACCAGA ACAGAAGGGC ACTTGAGCAT 
CAGAAGAAAG AGTTTGTGAA ACATTCGAAA AGCTTCAGCG ACACTCTGAA AACATACTTC 
AAAGACGGAA AGGCAATAAA CGTGTTTGCC AGTGCCAACC GGCTCATCCA CCAAACCAAC 
TTGATACTGC AGACCTTCAA AACTGTCGCA TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Evolution of complexity in the zebrafish synapse proteome.
Nature communications814613(2017 Mar)
Bay?s ?,Collins MO,Reig-Viader R,Gou G,Goulding D,Izquierdo A,Choudhary JS,Emes RD,Grant SG

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

First description of programmed cell death10 (PDCD10) in rock bream (Oplegnathus fasciatus): Potential relations to the regulation of apoptosis by several pathogens.
Developmental and comparative immunology5551-5(2016 Feb)
Kim JW,Jeong JM,Bae JS,Cho DH,Jung SH,Hwang JY,Kwon MG,Seo JS,Baeck GW,Park CI

Ccm3 functions in a manner distinct from Ccm1 and Ccm2 in a zebrafish model of CCM vascular disease.
Developmental biology362(2)121-31(2012 Feb)
Yoruk B,Gillers BS,Chi NC,Scott IC

CCM3/PDCD10 heterodimerizes with germinal center kinase III (GCKIII) proteins using a mechanism analogous to CCM3 homodimerization.
The Journal of biological chemistry286(28)25056-64(2011 Jul)
Ceccarelli DF,Laister RC,Mulligan VK,Kean MJ,Goudreault M,Scott IC,Derry WB,Chakrabartty A,Gingras AC,Sicheri F