adam10b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	adam10b
Entrez Gene ID	393392
Full Name	ADAM metallopeptidase domain 10b
Synonyms	wu:fc03d12,zgc:64203
General protein information	Preferred Names ADAM metallopeptidase domain 10b Names ADAM metallopeptidase domain 10b a disintegrin and metalloprotease domain 10b
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_200420.1	NP_956714.1	ADAM metallopeptidase domain 10b precursor
XM_021470558.1	XP_021326233.1	ADAM metallopeptidase domain 10b

Pathways from BioSystems

Homologies

Caenorhabditis elegans (roundworm)	sup-17	NP_492377.1
Drosophila melanogaster (fruit fly)	kuz	NP_001097160.2
Danio rerio (zebrafish)	adam10b	NP_956714.1
Anopheles gambiae (African malaria mosquito)	AgaP_AGAP008790	XP_314916.4

Gene Ontology
Bibliography

Related articles in PubMed

ADAM12-deficient zebrafish exhibit retardation in body growth at the juvenile stage without developmental defects.
Tokumasu Y, Iida A, Wang Z, Ansai S, Kinoshita M, Sehara-Fujisawa A
Development, growth & differentiation58(4)409-21(2016 May)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

Consequences of lineage-specific gene loss on functional evolution of surviving paralogs: ALDH1A and retinoic acid signaling in vertebrate genomes.
Ca?estro C, Catchen JM, Rodr?guez-Mar? A, Yokoi H, Postlethwait JH
PLoS genetics5(5)e1000496(2009 May)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following adam10b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the adam10b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa03794	NM_200420.1 Latest version!	Danio rerio ADAM metallopeptidase domain 10b (adam10b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00
ODa141369	XM_021470558.1 Latest version!	Danio rerio ADAM metallopeptidase domain 10b (adam10b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$426.30 $609.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

adam10b ( NM_200420.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_956714.1 Danio rerio ADAM metallopeptidase domain 10b (adam10b), mRNA.

Price & Availability↑

CloneID	ODa03794
Clone ID Related Accession (Same CDS sequence)	NM_200420.1
Accession Version	NM_200420.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2343bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	ADAM metallopeptidase domain 10b precursor
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC054638.1. ##Evidence-Data-START## Transcript exon combination :: BC054638.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGCGGCTGT CCGTGATGTC GGTGTTCCCG GTGCTCCCGG TGCTGCTGGG CCTCCTCTGC 
GCCTCCGGGC CCGCTCGAGG TTACTATGGA AACCCCCTGA ACAAGTACAT CCGGCACTAC 
GAGGGTCTTT CTTACGACAC GGAGATGCTG CACAGCGACC ACCAGAGGGC CAAACGCGCA 
GCCACACACA CCGAGCGCAC GCTGCAGCTG GAGTTCCACG CGCACGGCAG GCACTTTAAC 
CTGCGCATGA GGCGGGACAC CGGACTGTTT GCAGACGACT TCAAGCTGGA AGTTTCTGGA 
CAGGAGATGG ACTACGACAC CTCACACATC TACAGCGGAG AGATCTATGG TGAGCAGGGC 
ACACTCACCC ATGGCTCAGT GCAGGACGGG CGGTTCGAGG GCTTCATCCT GACCCACAGA 
GGGACGTTTT ACGTGGAGCC GGTGGAGCGA TACCTGCAGG ACAAGAACGT GCCCTTCCAC 
TCCGTCATCT ACCACGAGGA CGACATCGAT TATCCTCATA AGTACGGGCC GGCGGGCGGC 
TGTGCGGACG CCTCTGTGTT CCAGAAGATG AAGAGATACC AGAGCACTGC GCACCAGGAG 
CCCACGGACA GCCCCCCGGC GCAGGATGAA GATGTGCTGC TGAGGAAGAA GAGGATGACG 
CAGGTGGAGA AGAACACCTG TCAGCTCTTC ATCCAGACTG ACCACCTGTT CTACAAATAC 
TACAAGACCA GAGAGGCTGT CATCGCTCAG ATCTCCAGTC ATGTGAAGGC GATTGATGCT 
ATATATCAGG GCACAGACTT CATGGGCATC CGCAACATCA GCTTCATGGT GAAGAGGATT 
CGGATCAACA CCACCAGTGA TGAGCGTGAC CGCTCTAACC CGTTCCGCTT CGCTAACATC 
GGTGTGGAGA AGTTCCTGGA GCTGAACTCT GAGCAGAACC ATGATGACTA CTGCCTGGCC 
TACGTCTTCA CCGACAGAGA CTTCGATGAT GGAGTGCTGG GGCTGGCCTG GGTCGGAGCT 
CCCGCAGGGA GCTCTGGGGG TATCTGTGAG AAGAATAAGC TGTACTCTGA CGGTAAGAAG 
AAGTCCCTGA ACACCGGCAT CATCACGGTG CAGAACTACG CCTCACACGT GCCGCCCAAA 
GTCTCGCACA TCACCTTCGC TCACGAGGTC GGACACAACT TCGGCTCTCC GCACGACTCC 
GGCTCTGAGT GCACCCCCGG CGAGTCCAAG AGTCCGGATA AGAAGGAGCG CGGGAACTAC 
ATCATGTACG CTCGCGCCAC GTCCGGAGAC AAACTCAACA ACAACAAGTT CTCCGTCTGC 
AGCATCAAGA ACATCAGCCA GGTGCTGGAG AAGAAGCGCG GCAGCTGTTT CGTGGAGTCC 
GGGCAGCCGA TCTGTGGGAA CGGGCTGGTG GAGCCGGGTG AGCAGTGTGA CTGTGGATAC 
AGCGATCAGT GTAAAGACGA CTGCTGCTAC GATGCTAATC AGCCCGACAA CAAGAAGTGC 
AAACTCAAAC CTGGGAAAGT GTGCAGCCCC AGTCAGGGCC CGTGCTGCAC GTTCGAGTGC 
TCCTATAAGG GTCATAATGA GAAGTGTCGT GAGGAGTCTG AGTGTGCTCA TCAGGGTCTG 
TGCAGCGGCT CCTCGGCCCA GTGCCCCACC TCTGAACCCA AGGCCAACTT CACCGCCTGC 
CACGGGAACA CACAGGTCTG CCTCAACGGG GGCTGCTCCG GCTCCATCTG TGAGAAGTAC 
GGTCTGGAGG TCTGCACCTG CGCCAGTGTT GAAGGCAAAG ACGAGACGGA GCTCTGCCAC 
GTCTGCTGCA TGGAGAAGAT GAACCCGAGC ACCTGCAGCA GCACCGGCTC GGAGCGTCTG 
TCCCGGTTCT TCGGGAAGCG TGTGATCACG CTGCAGGCCG GCTCTCCCTG TAATGACTTT 
AAGGGTTACT GTGACGTCTT CATGAAGTGC CGGCTGGTGG ACGCTGACGG GCCGCTCGCC 
CGACTCAAGA AGGCCATCTT CAACCCCGAG CTCTACGAGA ACATCGCCGA GTGGATGATG 
GCTCACTGGT GGGCGGTTCT GCTGATGGGC ATCGCTCTGA TCATGCTCAT GGCGGGCTTC 
ATCAAGATCT GCAGCGTTCA CACACCCAGC AGCAACCCCA AACTGCCTCC ACCCAAACCA 
CTTCCAGGTA CGCTGAAGCG TCGGCGAGCG CAGCACTCGA GTCAGCACCC TCCTCCTTCA 
CACCAGCAGG CGGTGCCGCA CGCTCACGCC CTCCCGAACC CACGGCCCGC CCCGCGCCCG 
CAGCCAGCCC GCCACCACCG CTCCGCCCGC GAGAACTACC AGATGGGGCA GATGCGGCGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_956714.1
CDS	57..2399
Translation	MRLSVMSVFPVLPVLLGLLCASGPARGYYGNPLNKYIRHYEGLSYDTEMLHSDHQRAKRAATHTERTLQLEFHAHGRHFNLRMRRDTGLFADDFKLEVSGQEMDYDTSHIYSGEIYGEQGTLTHGSVQDGRFEGFILTHRGTFYVEPVERYLQDKNVPFHSVIYHEDDIDYPHKYGPAGGCADASVFQKMKRYQSTAHQEPTDSPPAQDEDVLLRKKRMTQVEKNTCQLFIQTDHLFYKYYKTREAVIAQISSHVKAIDAIYQGTDFMGIRNISFMVKRIRINTTSDERDRSNPFRFANIGVEKFLELNSEQNHDDYCLAYVFTDRDFDDGVLGLAWVGAPAGSSGGICEKNKLYSDGKKKSLNTGIITVQNYASHVPPKVSHITFAHEVGHNFGSPHDSGSECTPGESKSPDKKERGNYIMYARATSGDKLNNNKFSVCSIKNISQVLEKKRGSCFVESGQPICGNGLVEPGEQCDCGYSDQCKDDCCYDANQPDNKKCKLKPGKVCSPSQGPCCTFECSYKGHNEKCREESECAHQGLCSGSSAQCPTSEPKANFTACHGNTQVCLNGGCSGSICEKYGLEVCTCASVEGKDETELCHVCCMEKMNPSTCSSTGSERLSRFFGKRVITLQAGSPCNDFKGYCDVFMKCRLVDADGPLARLKKAIFNPELYENIAEWMMAHWWAVLLMGIALIMLMAGFIKICSVHTPSSNPKLPPPKPLPGTLKRRRAQHSSQHPPPSHQQAVPHAHALPNPRPAPRPQPARHHRSARENYQMGQMRR

Target ORF information:

RefSeq Version	NM_200420.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ADAM metallopeptidase domain 10b (adam10b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_200420.1

ORF Insert Sequence:

ATGCGGCTGT CCGTGATGTC GGTGTTCCCG GTGCTCCCGG TGCTGCTGGG CCTCCTCTGC 
GCCTCCGGGC CCGCTCGAGG TTACTATGGA AACCCCCTGA ACAAGTACAT CCGGCACTAC 
GAGGGTCTTT CTTACGACAC GGAGATGCTG CACAGCGACC ACCAGAGGGC CAAACGCGCA 
GCCACACACA CCGAGCGCAC GCTGCAGCTG GAGTTCCACG CGCACGGCAG GCACTTTAAC 
CTGCGCATGA GGCGGGACAC CGGACTGTTT GCAGACGACT TCAAGCTGGA AGTTTCTGGA 
CAGGAGATGG ACTACGACAC CTCACACATC TACAGCGGAG AGATCTATGG TGAGCAGGGC 
ACACTCACCC ATGGCTCAGT GCAGGACGGG CGGTTCGAGG GCTTCATCCT GACCCACAGA 
GGGACGTTTT ACGTGGAGCC GGTGGAGCGA TACCTGCAGG ACAAGAACGT GCCCTTCCAC 
TCCGTCATCT ACCACGAGGA CGACATCGAT TATCCTCATA AGTACGGGCC GGCGGGCGGC 
TGTGCGGACG CCTCTGTGTT CCAGAAGATG AAGAGATACC AGAGCACTGC GCACCAGGAG 
CCCACGGACA GCCCCCCGGC GCAGGATGAA GATGTGCTGC TGAGGAAGAA GAGGATGACG 
CAGGTGGAGA AGAACACCTG TCAGCTCTTC ATCCAGACTG ACCACCTGTT CTACAAATAC 
TACAAGACCA GAGAGGCTGT CATCGCTCAG ATCTCCAGTC ATGTGAAGGC GATTGATGCT 
ATATATCAGG GCACAGACTT CATGGGCATC CGCAACATCA GCTTCATGGT GAAGAGGATT 
CGGATCAACA CCACCAGTGA TGAGCGTGAC CGCTCTAACC CGTTCCGCTT CGCTAACATC 
GGTGTGGAGA AGTTCCTGGA GCTGAACTCT GAGCAGAACC ATGATGACTA CTGCCTGGCC 
TACGTCTTCA CCGACAGAGA CTTCGATGAT GGAGTGCTGG GGCTGGCCTG GGTCGGAGCT 
CCCGCAGGGA GCTCTGGGGG TATCTGTGAG AAGAATAAGC TGTACTCTGA CGGTAAGAAG 
AAGTCCCTGA ACACCGGCAT CATCACGGTG CAGAACTACG CCTCACACGT GCCGCCCAAA 
GTCTCGCACA TCACCTTCGC TCACGAGGTC GGACACAACT TCGGCTCTCC GCACGACTCC 
GGCTCTGAGT GCACCCCCGG CGAGTCCAAG AGTCCGGATA AGAAGGAGCG CGGGAACTAC 
ATCATGTACG CTCGCGCCAC GTCCGGAGAC AAACTCAACA ACAACAAGTT CTCCGTCTGC 
AGCATCAAGA ACATCAGCCA GGTGCTGGAG AAGAAGCGCG GCAGCTGTTT CGTGGAGTCC 
GGGCAGCCGA TCTGTGGGAA CGGGCTGGTG GAGCCGGGTG AGCAGTGTGA CTGTGGATAC 
AGCGATCAGT GTAAAGACGA CTGCTGCTAC GATGCTAATC AGCCCGACAA CAAGAAGTGC 
AAACTCAAAC CTGGGAAAGT GTGCAGCCCC AGTCAGGGCC CGTGCTGCAC GTTCGAGTGC 
TCCTATAAGG GTCATAATGA GAAGTGTCGT GAGGAGTCTG AGTGTGCTCA TCAGGGTCTG 
TGCAGCGGCT CCTCGGCCCA GTGCCCCACC TCTGAACCCA AGGCCAACTT CACCGCCTGC 
CACGGGAACA CACAGGTCTG CCTCAACGGG GGCTGCTCCG GCTCCATCTG TGAGAAGTAC 
GGTCTGGAGG TCTGCACCTG CGCCAGTGTT GAAGGCAAAG ACGAGACGGA GCTCTGCCAC 
GTCTGCTGCA TGGAGAAGAT GAACCCGAGC ACCTGCAGCA GCACCGGCTC GGAGCGTCTG 
TCCCGGTTCT TCGGGAAGCG TGTGATCACG CTGCAGGCCG GCTCTCCCTG TAATGACTTT 
AAGGGTTACT GTGACGTCTT CATGAAGTGC CGGCTGGTGG ACGCTGACGG GCCGCTCGCC 
CGACTCAAGA AGGCCATCTT CAACCCCGAG CTCTACGAGA ACATCGCCGA GTGGATGATG 
GCTCACTGGT GGGCGGTTCT GCTGATGGGC ATCGCTCTGA TCATGCTCAT GGCGGGCTTC 
ATCAAGATCT GCAGCGTTCA CACACCCAGC AGCAACCCCA AACTGCCTCC ACCCAAACCA 
CTTCCAGGTA CGCTGAAGCG TCGGCGAGCG CAGCACTCGA GTCAGCACCC TCCTCCTTCA 
CACCAGCAGG CGGTGCCGCA CGCTCACGCC CTCCCGAACC CACGGCCCGC CCCGCGCCCG 
CAGCCAGCCC GCCACCACCG CTCCGCCCGC GAGAACTACC AGATGGGGCA GATGCGGCGC 
TGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

adam10b ( XM_021470558.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326233.1 Danio rerio ADAM metallopeptidase domain 10b (adam10b), mRNA.

Price & Availability↑

CloneID	ODa141369
Clone ID Related Accession (Same CDS sequence)	XM_021470558.1
Accession Version	XM_021470558.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2010bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	ADAM metallopeptidase domain 10b
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCGGCTGT CCGTGATGTC GGTGTTCCCG GTGCTCCCGG TGCTGCTGGG CCTCCTCTGC 
GCCTCCGGGC CCGCTCGAGG TTACTATGGA AACCCCCTGA ACAAGTACAT CCGGCACTAC 
GAGGGTCTTT CTTACGACAC GGAGATGCTG CACAGCGACC ACCAGAGGGC CAAACGCGCA 
GCCACACACA CCGAGCGCAC GCTGCAGCTG GAGTTCCACG CGCACGGCAG GCACTTTAAC 
CTGCGCATGA GGCGGGACAC CGGACTGTTT GCAGACGACT TCAAGCTGGA AGTTTCTGGA 
CAGGAGATGG ACTACGACAC CTCACACATC TACAGCGGAG AGATCTATGG TGAGCAGGGC 
ACACTCACCC ATGGCTCAGT GCAGGACGGG CGGTTCGAGG GCTTCATCCT GACCCACAGA 
GGGACGTTTT ACGTGGAGCC GGTGGAGCGA TACCTGCAGG ACAAGAACGT GCCCTTCCAC 
TCCGTCATCT ACCACGAGGA CGACATCGAT TATCCTCATA AGTACGGGCC GGCGGGCGNG 
TGTGTGTGTG TGTGTGTGTG TGAGTCCTCT AACGCTGTAC CCCCTGCTCA TCTTCCTCCT 
CTTGATCTCC TTCTTCAGGA CAGCCCCCCG GCGCAGGATG AAGATGTGCT GCTGAGGAAG 
AAGAGGATGA CGCAGGTGGA GAAGAACACC TGTCAGCTCT TCATCCAGAC TGACCACCTG 
TTCTACAAAT ACTACAAGAC CAGAGAGGCT GTCATCGCTC AGATCTCCAG TCATGTGAAG 
GCGATTGATG CTATATATCA GGGCACAGAC TTCATGGGCA TCCGCAACAT CAGCTTCATG 
GTGAAGAGGA TTCGGATCAA CACCACCAGT GATGAGCGTG ACCGCTCTAA CCCGTTCCGC 
TTCGCTAACA TCGGTGTGGA GAAGTTCCTG GAGCTGAACT CTGAGCAGAA CCATGATGAC 
TACTGCCTGG CCTACGTCTT CACCGACAGA GACTTCGATG ATGGAGTGCT GGGGCTGGCC 
TGGGTCGGAG CTCCCGCAGG GAGCTCTGGG GGTATCTGTG AGAAGAATAA GCTGTACTCT 
GACGGTAAGA AGAAGTCCCT GAACACCGGC ATCATCACGG TGCAGAACTA CGCCTCACAC 
GTGCCGCCCA AAGTCTCGCA CATCACCTTC GCTCACGAGG TCGGACACAA CTTCGGCTCT 
CCGCACGACT CCGGCTCTGA GTGCACCCCC GGCGAGTCCA AGAGTCCGGA TAAGAAGGAG 
CGCGGGAACT ACATCATGTA CGCTCGCGCC ACGTCCGGAG ACAAACTCAA CAACAACAAG 
TTCTCCGTCT GCAGCATCAA GAACATCAGC CAGGTGCTGG AGAAGAAGCG CGGCAGCTGT 
TTCGTGGAGT CCGGGCAGCC GATCTGTGGG AACGGGCTGG TGGAGCCGGG TGAGCAGTGT 
GACTGTGGAT ACAGCGATCA GTGTAAAGAC GACTGCTGCT ACGATGCTAA TCAGCCCGAC 
AACAAGAAGT GCAAACTCAA ACCTGGGAAA GTGTGCAGCC CCAGTCAGGG CCCGTGCTGC 
ACGTTCGAGT GCTCCTATAA GGGTCATAAT GAGAAGTGTC GTGAGGAGTC TGAGTGTGCT 
CATCAGGGTC TGTGCAGCGG CTCCTCGGCC CAGTGCCCCA CCTCTGAACC CAAGGCCAAC 
TTCACCGCCT GCCACGGGAA CACACAGGTC TGCCTCAACG GGGGCTGCTC CGGCTCCATC 
TGTGAGAAGT ACGGTCTGGA GGTCTGCACC TGCGCCAGTG TTGAAGGCAA AGACGAGACG 
GAGCTCTGCC ACGTCTGCTG CATGGAGAAG AGTCAGTACA CACACACACA CACACACACA 
CACACACACA CACACACACA CACTTCCGCA TCGCATGAAG TGGTGCAGCA CAGCCAGACC 
TGCTCACAGT GTAGAGCACA CAGAACTCCC GCTCAGAGTG TGTGTGTGTG TGTGTTTACC 
AGTAATCACA CTACATCTGT TACACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326233.1
CDS	103..2112
Translation	MRLSVMSVFPVLPVLLGLLCASGPARGYYGNPLNKYIRHYEGLSYDTEMLHSDHQRAKRAATHTERTLQLEFHAHGRHFNLRMRRDTGLFADDFKLEVSGQEMDYDTSHIYSGEIYGEQGTLTHGSVQDGRFEGFILTHRGTFYVEPVERYLQDKNVPFHSVIYHEDDIDYPHKYGPAGnullCVCVCVCESSNAVPPAHLPPLDLLLQDSPPAQDEDVLLRKKRMTQVEKNTCQLFIQTDHLFYKYYKTREAVIAQISSHVKAIDAIYQGTDFMGIRNISFMVKRIRINTTSDERDRSNPFRFANIGVEKFLELNSEQNHDDYCLAYVFTDRDFDDGVLGLAWVGAPAGSSGGICEKNKLYSDGKKKSLNTGIITVQNYASHVPPKVSHITFAHEVGHNFGSPHDSGSECTPGESKSPDKKERGNYIMYARATSGDKLNNNKFSVCSIKNISQVLEKKRGSCFVESGQPICGNGLVEPGEQCDCGYSDQCKDDCCYDANQPDNKKCKLKPGKVCSPSQGPCCTFECSYKGHNEKCREESECAHQGLCSGSSAQCPTSEPKANFTACHGNTQVCLNGGCSGSICEKYGLEVCTCASVEGKDETELCHVCCMEKSQYTHTHTHTHTHTHTHTSASHEVVQHSQTCSQCRAHRTPAQSVCVCVFTSNHTTSVTQ

Target ORF information:

RefSeq Version	XM_021470558.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ADAM metallopeptidase domain 10b (adam10b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470558.1

ORF Insert Sequence:

ATGCGGCTGT CCGTGATGTC GGTGTTCCCG GTGCTCCCGG TGCTGCTGGG CCTCCTCTGC 
GCCTCCGGGC CCGCTCGAGG TTACTATGGA AACCCCCTGA ACAAGTACAT CCGGCACTAC 
GAGGGTCTTT CTTACGACAC GGAGATGCTG CACAGCGACC ACCAGAGGGC CAAACGCGCA 
GCCACACACA CCGAGCGCAC GCTGCAGCTG GAGTTCCACG CGCACGGCAG GCACTTTAAC 
CTGCGCATGA GGCGGGACAC CGGACTGTTT GCAGACGACT TCAAGCTGGA AGTTTCTGGA 
CAGGAGATGG ACTACGACAC CTCACACATC TACAGCGGAG AGATCTATGG TGAGCAGGGC 
ACACTCACCC ATGGCTCAGT GCAGGACGGG CGGTTCGAGG GCTTCATCCT GACCCACAGA 
GGGACGTTTT ACGTGGAGCC GGTGGAGCGA TACCTGCAGG ACAAGAACGT GCCCTTCCAC 
TCCGTCATCT ACCACGAGGA CGACATCGAT TATCCTCATA AGTACGGGCC GGCGGGCGNG 
TGTGTGTGTG TGTGTGTGTG TGAGTCCTCT AACGCTGTAC CCCCTGCTCA TCTTCCTCCT 
CTTGATCTCC TTCTTCAGGA CAGCCCCCCG GCGCAGGATG AAGATGTGCT GCTGAGGAAG 
AAGAGGATGA CGCAGGTGGA GAAGAACACC TGTCAGCTCT TCATCCAGAC TGACCACCTG 
TTCTACAAAT ACTACAAGAC CAGAGAGGCT GTCATCGCTC AGATCTCCAG TCATGTGAAG 
GCGATTGATG CTATATATCA GGGCACAGAC TTCATGGGCA TCCGCAACAT CAGCTTCATG 
GTGAAGAGGA TTCGGATCAA CACCACCAGT GATGAGCGTG ACCGCTCTAA CCCGTTCCGC 
TTCGCTAACA TCGGTGTGGA GAAGTTCCTG GAGCTGAACT CTGAGCAGAA CCATGATGAC 
TACTGCCTGG CCTACGTCTT CACCGACAGA GACTTCGATG ATGGAGTGCT GGGGCTGGCC 
TGGGTCGGAG CTCCCGCAGG GAGCTCTGGG GGTATCTGTG AGAAGAATAA GCTGTACTCT 
GACGGTAAGA AGAAGTCCCT GAACACCGGC ATCATCACGG TGCAGAACTA CGCCTCACAC 
GTGCCGCCCA AAGTCTCGCA CATCACCTTC GCTCACGAGG TCGGACACAA CTTCGGCTCT 
CCGCACGACT CCGGCTCTGA GTGCACCCCC GGCGAGTCCA AGAGTCCGGA TAAGAAGGAG 
CGCGGGAACT ACATCATGTA CGCTCGCGCC ACGTCCGGAG ACAAACTCAA CAACAACAAG 
TTCTCCGTCT GCAGCATCAA GAACATCAGC CAGGTGCTGG AGAAGAAGCG CGGCAGCTGT 
TTCGTGGAGT CCGGGCAGCC GATCTGTGGG AACGGGCTGG TGGAGCCGGG TGAGCAGTGT 
GACTGTGGAT ACAGCGATCA GTGTAAAGAC GACTGCTGCT ACGATGCTAA TCAGCCCGAC 
AACAAGAAGT GCAAACTCAA ACCTGGGAAA GTGTGCAGCC CCAGTCAGGG CCCGTGCTGC 
ACGTTCGAGT GCTCCTATAA GGGTCATAAT GAGAAGTGTC GTGAGGAGTC TGAGTGTGCT 
CATCAGGGTC TGTGCAGCGG CTCCTCGGCC CAGTGCCCCA CCTCTGAACC CAAGGCCAAC 
TTCACCGCCT GCCACGGGAA CACACAGGTC TGCCTCAACG GGGGCTGCTC CGGCTCCATC 
TGTGAGAAGT ACGGTCTGGA GGTCTGCACC TGCGCCAGTG TTGAAGGCAA AGACGAGACG 
GAGCTCTGCC ACGTCTGCTG CATGGAGAAG AGTCAGTACA CACACACACA CACACACACA 
CACACACACA CACACACACA CACTTCCGCA TCGCATGAAG TGGTGCAGCA CAGCCAGACC 
TGCTCACAGT GTAGAGCACA CAGAACTCCC GCTCAGAGTG TGTGTGTGTG TGTGTTTACC 
AGTAATCACA CTACATCTGT TACACAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

ADAM12-deficient zebrafish exhibit retardation in body growth at the juvenile stage without developmental defects.
Development, growth & differentiation58(4)409-21(2016 May)
Tokumasu Y,Iida A,Wang Z,Ansai S,Kinoshita M,Sehara-Fujisawa A

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

Consequences of lineage-specific gene loss on functional evolution of surviving paralogs: ALDH1A and retinoic acid signaling in vertebrate genomes.
PLoS genetics5(5)e1000496(2009 May)
Ca?estro C,Catchen JM,Rodr?guez-Mar? A,Yokoi H,Postlethwait JH

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adam10b cDNA ORF clone, Danio rerio(zebrafish)

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