npas2 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	npas2
Entrez Gene ID	352934
Full Name	neuronal PAS domain protein 2
Synonyms	zfCLOCK2
General protein information	Preferred Names neuronal PAS domain protein 2 Names circadian clock protein PASD1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005165121.4	XP_005165178.1	circadian clock protein PASD1 isoform X1
XM_021476023.1	XP_021331698.1	circadian clock protein PASD1 isoform X1
XM_005165120.3	XP_005165177.1	circadian clock protein PASD1 isoform X1
NM_178299.1	NP_840084.1	circadian clock protein PASD1
XM_009301290.2	XP_009299565.1	circadian clock protein PASD1 isoform X1
XM_005165121.3	XP_005165178.1	circadian clock protein PASD1 isoform X1
XM_005165120.4	XP_005165177.1	circadian clock protein PASD1 isoform X1

Pathways from BioSystems

Homologies

Gallus gallus (chicken)	PASD1	XP_004940908.1
Danio rerio (zebrafish)	npas2	NP_840084.1
Xenopus tropicalis (tropical clawed frog)	LOC100486786	XP_002934591.2

Gene Ontology
Bibliography

Related articles in PubMed

Clock1a affects mesoderm development and primitive hematopoiesis by regulating Nodal-Smad3 signaling in the zebrafish embryo.
Bian SS, Zheng XL, Sun HQ, Chen JH, Lu YL, Liu YQ, Tao DC, Ma YX
The Journal of biological chemistry292(34)14165-14175(2017 08)

A chemical screen in zebrafish embryonic cells establishes that Akt activation is required for neural crest development.
Ciarlo C, Kaufman CK, Kinikoglu B, Michael J, Yang S, D Amato C, Blokzijl-Franke S, den Hertog J, Schlaeger TM, Zhou Y, Liao E, Zon LI
eLife6(2017 Aug)

Transcriptional alterations induced by binary mixtures of ethinylestradiol and norgestrel during the early development of zebrafish (Danio rerio).
Liang YQ, Huang GY, Zhao JL, Shi WJ, Hu LX, Tian F, Liu SS, Jiang YX, Ying GG
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP19560-67(2017 May)

Genetically Blocking the Zebrafish Pineal Clock Affects Circadian Behavior.
Ben-Moshe Livne Z, Alon S, Vallone D, Bayleyen Y, Tovin A, Shainer I, Nisembaum LG, Aviram I, Smadja-Storz S, Fuentes M, Falc?n J, Eisenberg E, Klein DC, Burgess HA, Foulkes NS, Gothilf Y
PLoS genetics12(11)e1006445(2016 Nov)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following npas2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the npas2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa31272	NM_178299.1 Latest version!	Danio rerio neuronal PAS domain protein 2 (npas2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa10247	XM_005165121.4 Latest version!	Danio rerio neuronal PAS domain protein 2 (npas2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa10247	XM_021476023.1 Latest version!	Danio rerio neuronal PAS domain protein 2 (npas2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa10247	XM_005165120.4 Latest version!	Danio rerio neuronal PAS domain protein 2 (npas2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa10247	XM_009301290.2 Latest version!	Danio rerio neuronal PAS domain protein 2 (npas2), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
ODa10247	XM_005165121.3	Danio rerio neuronal PAS domain protein 2 (npas2), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa10247	XM_005165120.3	Danio rerio neuronal PAS domain protein 2 (npas2), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

ORF Online Only Promotion

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

npas2 ( NM_178299.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_840084.1 Danio rerio neuronal PAS domain protein 2 (npas2), mRNA.

Price & Availability↑

CloneID	ODa31272
Clone ID Related Accession (Same CDS sequence)	NM_178299.1
Accession Version	NM_178299.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-20
Organism	Danio rerio(zebrafish)
Product	circadian clock protein PASD1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AB087255.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AB087255.1, BC163256.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA4476752, SAMEA4476799 [ECO:0000348] ##Evidence-Data-END##

ATGGGAGAAG GAGTGAGCAT GGACAACCTT TCGGAGTTTG GAGGCCTTTG TCCGTCCAGC 
CGGAGGGAAT GGGACACAAG CAGTTGTGTG GATGATTTAA TGGACGAAGA TGAGAAGGAC 
AGAGCAAAAA GGGCGTCCCG GAACAAATCG GAGAAGAAGA GGCGAGACCA GTTCAATGTT 
CTCATTAAGG AGCTGTGTAC AATGCTGCAG GGTCAAGGTC ACCCCCGCAA GATGGACAAA 
TCCACCATCC TGCAGAGGAC CATCGACTTC CTGCAAAAAC AGAAAGAGAT CACGGCACAA 
ACAGAGTCTT GTGAGGTCCG GCAGGACTGG AAGCCTTCTT TCCTCAGCAA CGAGGAGTTC 
ACCCAGTTAA TGCTGGAGGC GTTGGATGGT TTTCTAATAG CTCTTACCAC AGATGGGAAC 
ATCATATATG TATCTGACAG CGTCTCGTCT CTCATTGGCC ATCTGCCGTC AGATATGGTG 
GACCAGAACA TCCTGAACTT CCTGCCAGAG CGAGAGCACG CAGATGTGTA TAAACTCCTG 
TCTTCACACA TGCTTCTGAC TGAGTCTTCT ACTGTAGACC TGCTCAACAG CAATGAGACA 
CACGTCGAGT TCTGCTGTCA TATAGCGAGG GGAAACATTG ACCCGAAAGA ACCGCCTACG 
TACGAATATG TCAAATTTGT TGGAGATTTC AAGTTTCATA ATAATGTGCC TCTGTCTTCA 
TGTAATGGTT ATGATCTGGC GTTTCCTCGC ACGCTGCAGT CCTCAATAGA GGAGCAGGTC 
TGCCTCGTGG CCACAGTCAG ACTCGCAACC CCTCAGTTCC TCAAGGATCT TTGCAACGTG 
GAGGATGTGT GTGATGAATT CACATCAAGA CACAGTCTGG AATGGAAATT TCTCTTCTTA 
GATCACAGGG CCTCTCCTAT CATTGGGTAC CTGCCGTTTG AGGTTTTGGG GACCTCGGGT 
TATGATTACT ACCATGTGGA CGACCTGGAG CTGATTGCTC AATGTCATAA ACAATTGATG 
CAGTGTGGGA AAGGAAAGTC TTGTTACTAT CGGTTCCTGA CTAAAGGACA GCAGTGGATC 
TGGCTGCAGA CGCACTATTA CATCACGTAC CACCAGTGGA ACTCCAAACC TGAGTTCATC 
GTCTGCACAC ACAGCGTGGT CAGTTATGCT GAAGTTCGAG CGGAGAGAAG GAGAGAGTTT 
GGATTGGAGG AGAGCTCATC TGATATGGCC ACATCCTCAA TCAAAGGCCA GGAGGTGTTT 
TTGGACATGT GTCCTCCTCT GGAAGCTACA CGGGACAGAA TCAACAGCGC TCGATCAGTG 
TCATCACAGA GCTCCAGAAA ATCATCACAC ACAGCGCTCT CCGACTCCGC ATCGAACTCC 
TCAGTGAGAT ACACAGAAGC ATGTACGTCA TCCCGGCAGT CTGCTTCCAT CGGCCCTGAG 
AAGAGCTCCA ACAGAATGAC ACACACTGGA ACAAAGACCC TGATTCAGAG ACAGAGCTCT 
TCAGAGCCCC CGTCACTCTC TCCGTCCTGC AGTCAGCATT CAGCCATGAC TCAGTCCCTG 
TACAGCTTCC AGCAGCCTCA GTTAGGTGTC ATGCATCAGC TAAAGGAGCA GCTGGAGGAG 
CGAACGCGGA TCCTACAGGC CGACATCAAA ACACAACAGC AAGAGCTGCA CGACATTAAA 
GAGAAGCTGC AGCTCGCCAA CCTACAGATG TTACTCCAGC AGCCGATACA GGGAGAGTTT 
GCTGGTTCTG GTCCACAGCA GCAGGGTCCA GGCAGGCCGG CTCAACAAAA CACACACCCT 
AAACCTCAAC ACTGTGTATC ACATTCATCA CACACACTAC AACCCAACAG CTCATCCACT 
CAGGGGCAGC AGAGGCTGGT TCAGGCTGGA CACATGCAGG CCGTCAGTGT TCCAATGCAG 
ACACACACCA GTCTGACCAC ACCCTTCTAC AGCAACCCCA TGATGTTCTC CCCCAACCAC 
GGACACCGAA GCCAACATGA CACACACTGC CAAAGAAACA CACACGCCGA CTACAGCCAG 
GACGGACAGC TACGGATGCT GCTAAACCAG CCAATGCAAA CACTTGTACA AGACAGCAAT 
GGGGGGCCCC AGTCTTCCCA ATGCACCTCA ACTATTCAAC AAACCAAATA TCCTCTGGAG 
CAGCAGATGA TGGCTCCTTC CTTCCCTGTG CAGCAGGTGA ACTGTAACGC TGTGCTAGTC 
CCCTCCCCCG TCTTCACCTC ACCAATCATG ATCCCCCATA ATAGTTTCAT CACCCATCAG 
ACCACCCCCA CCTACACGAC TCACCCGCCG GTTGCCCCAC ACAGCTTACC ACTACAGCAG 
CCTCAGCAGT TCTTTCAGAT GCAGCCTCAG GGTCTGATTC ACGGCACTCC CACTCAGGCC 
TTATTCCACA CCCCTAGTGT TCCACAGCAA AGCACTGTGG GATACGTACA GCCACAACAG 
CAGCAGCAGC AACAGCAGCA TCATCATCAC TCCCAGTCTG GCAACCTGTC GGACTACAGA 
AACATGCTCC CTCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_840084.1
CDS	1..2538
Translation	MGEGVSMDNLSEFGGLCPSSRREWDTSSCVDDLMDEDEKDRAKRASRNKSEKKRRDQFNVLIKELCTMLQGQGHPRKMDKSTILQRTIDFLQKQKEITAQTESCEVRQDWKPSFLSNEEFTQLMLEALDGFLIALTTDGNIIYVSDSVSSLIGHLPSDMVDQNILNFLPEREHADVYKLLSSHMLLTESSTVDLLNSNETHVEFCCHIARGNIDPKEPPTYEYVKFVGDFKFHNNVPLSSCNGYDLAFPRTLQSSIEEQVCLVATVRLATPQFLKDLCNVEDVCDEFTSRHSLEWKFLFLDHRASPIIGYLPFEVLGTSGYDYYHVDDLELIAQCHKQLMQCGKGKSCYYRFLTKGQQWIWLQTHYYITYHQWNSKPEFIVCTHSVVSYAEVRAERRREFGLEESSSDMATSSIKGQEVFLDMCPPLEATRDRINSARSVSSQSSRKSSHTALSDSASNSSVRYTEACTSSRQSASIGPEKSSNRMTHTGTKTLIQRQSSSEPPSLSPSCSQHSAMTQSLYSFQQPQLGVMHQLKEQLEERTRILQADIKTQQQELHDIKEKLQLANLQMLLQQPIQGEFAGSGPQQQGPGRPAQQNTHPKPQHCVSHSSHTLQPNSSSTQGQQRLVQAGHMQAVSVPMQTHTSLTTPFYSNPMMFSPNHGHRSQHDTHCQRNTHADYSQDGQLRMLLNQPMQTLVQDSNGGPQSSQCTSTIQQTKYPLEQQMMAPSFPVQQVNCNAVLVPSPVFTSPIMIPHNSFITHQTTPTYTTHPPVAPHSLPLQQPQQFFQMQPQGLIHGTPTQALFHTPSVPQQSTVGYVQPQQQQQQQQHHHHSQSGNLSDYRNMLPR

Target ORF information:

RefSeq Version	NM_178299.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neuronal PAS domain protein 2 (npas2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178299.1

ORF Insert Sequence:

ATGGGAGAAG GAGTGAGCAT GGACAACCTT TCGGAGTTTG GAGGCCTTTG TCCGTCCAGC 
CGGAGGGAAT GGGACACAAG CAGTTGTGTG GATGATTTAA TGGACGAAGA TGAGAAGGAC 
AGAGCAAAAA GGGCGTCCCG GAACAAATCG GAGAAGAAGA GGCGAGACCA GTTCAATGTT 
CTCATTAAGG AGCTGTGTAC AATGCTGCAG GGTCAAGGTC ACCCCCGCAA GATGGACAAA 
TCCACCATCC TGCAGAGGAC CATCGACTTC CTGCAAAAAC AGAAAGAGAT CACGGCACAA 
ACAGAGTCTT GTGAGGTCCG GCAGGACTGG AAGCCTTCTT TCCTCAGCAA CGAGGAGTTC 
ACCCAGTTAA TGCTGGAGGC GTTGGATGGT TTTCTAATAG CTCTTACCAC AGATGGGAAC 
ATCATATATG TATCTGACAG CGTCTCGTCT CTCATTGGCC ATCTGCCGTC AGATATGGTG 
GACCAGAACA TCCTGAACTT CCTGCCAGAG CGAGAGCACG CAGATGTGTA TAAACTCCTG 
TCTTCACACA TGCTTCTGAC TGAGTCTTCT ACTGTAGACC TGCTCAACAG CAATGAGACA 
CACGTCGAGT TCTGCTGTCA TATAGCGAGG GGAAACATTG ACCCGAAAGA ACCGCCTACG 
TACGAATATG TCAAATTTGT TGGAGATTTC AAGTTTCATA ATAATGTGCC TCTGTCTTCA 
TGTAATGGTT ATGATCTGGC GTTTCCTCGC ACGCTGCAGT CCTCAATAGA GGAGCAGGTC 
TGCCTCGTGG CCACAGTCAG ACTCGCAACC CCTCAGTTCC TCAAGGATCT TTGCAACGTG 
GAGGATGTGT GTGATGAATT CACATCAAGA CACAGTCTGG AATGGAAATT TCTCTTCTTA 
GATCACAGGG CCTCTCCTAT CATTGGGTAC CTGCCGTTTG AGGTTTTGGG GACCTCGGGT 
TATGATTACT ACCATGTGGA CGACCTGGAG CTGATTGCTC AATGTCATAA ACAATTGATG 
CAGTGTGGGA AAGGAAAGTC TTGTTACTAT CGGTTCCTGA CTAAAGGACA GCAGTGGATC 
TGGCTGCAGA CGCACTATTA CATCACGTAC CACCAGTGGA ACTCCAAACC TGAGTTCATC 
GTCTGCACAC ACAGCGTGGT CAGTTATGCT GAAGTTCGAG CGGAGAGAAG GAGAGAGTTT 
GGATTGGAGG AGAGCTCATC TGATATGGCC ACATCCTCAA TCAAAGGCCA GGAGGTGTTT 
TTGGACATGT GTCCTCCTCT GGAAGCTACA CGGGACAGAA TCAACAGCGC TCGATCAGTG 
TCATCACAGA GCTCCAGAAA ATCATCACAC ACAGCGCTCT CCGACTCCGC ATCGAACTCC 
TCAGTGAGAT ACACAGAAGC ATGTACGTCA TCCCGGCAGT CTGCTTCCAT CGGCCCTGAG 
AAGAGCTCCA ACAGAATGAC ACACACTGGA ACAAAGACCC TGATTCAGAG ACAGAGCTCT 
TCAGAGCCCC CGTCACTCTC TCCGTCCTGC AGTCAGCATT CAGCCATGAC TCAGTCCCTG 
TACAGCTTCC AGCAGCCTCA GTTAGGTGTC ATGCATCAGC TAAAGGAGCA GCTGGAGGAG 
CGAACGCGGA TCCTACAGGC CGACATCAAA ACACAACAGC AAGAGCTGCA CGACATTAAA 
GAGAAGCTGC AGCTCGCCAA CCTACAGATG TTACTCCAGC AGCCGATACA GGGAGAGTTT 
GCTGGTTCTG GTCCACAGCA GCAGGGTCCA GGCAGGCCGG CTCAACAAAA CACACACCCT 
AAACCTCAAC ACTGTGTATC ACATTCATCA CACACACTAC AACCCAACAG CTCATCCACT 
CAGGGGCAGC AGAGGCTGGT TCAGGCTGGA CACATGCAGG CCGTCAGTGT TCCAATGCAG 
ACACACACCA GTCTGACCAC ACCCTTCTAC AGCAACCCCA TGATGTTCTC CCCCAACCAC 
GGACACCGAA GCCAACATGA CACACACTGC CAAAGAAACA CACACGCCGA CTACAGCCAG 
GACGGACAGC TACGGATGCT GCTAAACCAG CCAATGCAAA CACTTGTACA AGACAGCAAT 
GGGGGGCCCC AGTCTTCCCA ATGCACCTCA ACTATTCAAC AAACCAAATA TCCTCTGGAG 
CAGCAGATGA TGGCTCCTTC CTTCCCTGTG CAGCAGGTGA ACTGTAACGC TGTGCTAGTC 
CCCTCCCCCG TCTTCACCTC ACCAATCATG ATCCCCCATA ATAGTTTCAT CACCCATCAG 
ACCACCCCCA CCTACACGAC TCACCCGCCG GTTGCCCCAC ACAGCTTACC ACTACAGCAG 
CCTCAGCAGT TCTTTCAGAT GCAGCCTCAG GGTCTGATTC ACGGCACTCC CACTCAGGCC 
TTATTCCACA CCCCTAGTGT TCCACAGCAA AGCACTGTGG GATACGTACA GCCACAACAG 
CAGCAGCAGC AACAGCAGCA TCATCATCAC TCCCAGTCTG GCAACCTGTC GGACTACAGA 
AACATGCTCC CTCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

npas2 ( XM_005165121.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165178.1 Danio rerio neuronal PAS domain protein 2 (npas2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10247
Clone ID Related Accession (Same CDS sequence)	XM_005165121.4 , XM_021476023.1 , XM_005165120.3 , XM_009301290.2 , XM_005165121.3 , XM_005165120.4
Accession Version	XM_005165121.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	circadian clock protein PASD1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165121.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGAAG GAGTGAGCAT GGACAACCTT TCGGAGTTTG GAGGCCTTTG TCCGTCCAGC 
CGGAGGGAAT GGGACACAAG CAGTTGTGTG GATGATTTAA TGGACGAAGA TGAGAAGGAC 
AGAGCAAAAA GGGCGTCCCG GAACAAATCG GAGAAGAAGA GGCGAGACCA GTTCAATGTT 
CTCATTAAGG AGCTGTGTAC AATGCTGCAG GGTCAAGGTC ACCCCCGCAA GATGGACAAA 
TCCACCATCC TGCAGAGGAC CATCGACTTC CTGCAAAAAC AGAAAGAGAT CACGGCACAA 
ACAGAGTCTT GTGAGGTCCG GCAGGACTGG AAGCCTTCTT TCCTCAGCAA CGAGGAGTTC 
ACCCAGTTAA TGCTGGAGGC GTTGGATGGT TTTCTAATAG CTCTTACCAC AGATGGGAAC 
ATCATATATG TATCTGACAG CGTCTCGTCT CTCATTGGCC ATCTGCCGTC AGATATGGTG 
GACCAGAACA TCCTGAACTT CCTGCCAGAG CGAGAGCACG CAGATGTGTA TAAACTCCTG 
TCTTCACACA TGCTTCTGAC TGAGTCTTCT ACTGTAGACC TGCTCAACAG CAATGAGACA 
CACGTCGAGT TCTGCTGTCA TATAGCGAGG GGAAACATTG ACCCGAAAGA ACCGCCTACG 
TACGAATATG TCAAATTTGT TGGAGATTTC AAGTTTCATA ATAATGTGCC TCTGTCTTCA 
TGTAATGGTT ATGATCTGGC GTTTCCTCGC ACGCTGCAGT CCTCAATAGA GGAGCAGGTC 
TGCCTCGTGG CCACAGTCAG ACTCGCAACC CCTCAGTTCC TCAAGGATCT TTGCAACGTG 
GAGGATGTGT GTGATGAATT CACATCAAGA CACAGTCTGG AATGGAAATT TCTCTTCTTA 
GATCACAGGG CCTCTCCTAT CATCGGGTAC CTGCCGTTTG AGGTTTTGGG GACCTCGGGT 
TATGATTACT ACCATGTGGA CGACCTGGAG CTGATTGCTC AATGTCATAA ACAATTGATG 
CAGTGTGGGA AAGGAAAGTC TTGTTACTAT CGGTTCCTGA CTAAAGGACA GCAGTGGATC 
TGGCTGCAGA CGCACTATTA CATCACGTAC CACCAGTGGA ACTCCAAACC TGAGTTCATC 
GTCTGCACAC ACAGCGTGGT CAGTTATGCT GAAGTTCGAG CGGAGAGAAG GAGAGAGTTT 
GGATTGGAGG AGAGCTCATC TGATATGGCC ACATCCTCAA TCAAAGGCCA GGAGGTGTTT 
TTGGACATGT GTCCTCCTCT GGAAGCTACA CGGGACAGAA TCAACAGCGC TCGATCAGTG 
TCATCACAGA GCTCCAGAAA ATCATCACAC ACAGCGCTCT CCGACTCCGC ATCGAACTCC 
TCAGTGAGAT ACACAGAAGC ATGTACGTCA TCCCGGCAGT CTGCTTCCAT CGGCCCTGAG 
AAGAGCTCCA ACAGAATGAC ACACACTGGA ACAAAGACCC TGATTCAGAG ACAGAGCTCT 
TCAGAGCCCC CGTCACTCTC TCCGTCCTGC AGTCAGCATT CAGCCATGAC TCAGTCCCTG 
TACAGCTTCC AGCAGCCTCA GTTAGGTGTC ATGCATCAGC TAAAGGAGCA GCTGGAGGAG 
CGAACGCGGA TCCTACAGGC CGACATCAAA ACACAACAGC AAGAGCTGCA CGACATTAAA 
GAGAAGCTGC AGCTCGCCAA CCTACAGATG TTACTCCAGC AGCCGATACA GGGAGAGTTT 
GCTGGTTCTG GTCCACAGCA GCAGGGTCCA GGCAGGCCGG CTCAACAAAA CACACACCCT 
AAACCTCAAC ACTGTGTTTC ACATTCATCA CACACACTAC AACCCAACAG CTCATCCACT 
CAGGGGCAGC AGAGGCTGGT TCAGGCTGGA CACATGCAGG CCGTCAGTGT TCCAATGCAG 
ACACACACCA GTCTGACCAC ACCCTTCTAC AGCAACCCCA TGATGTTCTC CCCCAACCAC 
GGACACCGAA GCCAACATGA CACACACTGC CAAAGAAACA CACACGCCGA CTACAGCCAG 
GACGGACAGC TACGGATGCT GCTAAACCAG CCAATGCAAA CACTTGTACA AGACAGCAAT 
GGGGGGCCCC AGTCTTCCCA ATGCACCTCA ACTATTCAAC AAACCAAATA TCCTCTGGAG 
CAGCAGATGA TGGCTCCTTC CTTCCCTGTG CAGCAGGTGA ACTGTAACGC TGTGCTAGTC 
CCCTCCCCCG TCTTCACCTC ACCAATCATG ATCCCCCATA ATAGTTTCAT CACCCATCAG 
ACCACCCCCA CCTACACGAC TCACCCGCCG GTTGCCCCAC ACAGCTTACC ACTACAGCAG 
CCTCAGCAGT TCTTTCAGAT GCAGCCTCAG GGTCTGATTC ACGGCACTCC CACTCAGGCC 
TTATTCCACA CCCCTAGTGT TCCACAGCAA AGCACTGTGG GATACGTACA GCCACAACAG 
CAGCAGCAGC AACAGCAGCA TCATCATCAC TCCCAGTCTG GCAACCTGTC GGACTACAGA 
AACATGCTCC CTCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165178.1
CDS	929..3466
Translation	MGEGVSMDNLSEFGGLCPSSRREWDTSSCVDDLMDEDEKDRAKRASRNKSEKKRRDQFNVLIKELCTMLQGQGHPRKMDKSTILQRTIDFLQKQKEITAQTESCEVRQDWKPSFLSNEEFTQLMLEALDGFLIALTTDGNIIYVSDSVSSLIGHLPSDMVDQNILNFLPEREHADVYKLLSSHMLLTESSTVDLLNSNETHVEFCCHIARGNIDPKEPPTYEYVKFVGDFKFHNNVPLSSCNGYDLAFPRTLQSSIEEQVCLVATVRLATPQFLKDLCNVEDVCDEFTSRHSLEWKFLFLDHRASPIIGYLPFEVLGTSGYDYYHVDDLELIAQCHKQLMQCGKGKSCYYRFLTKGQQWIWLQTHYYITYHQWNSKPEFIVCTHSVVSYAEVRAERRREFGLEESSSDMATSSIKGQEVFLDMCPPLEATRDRINSARSVSSQSSRKSSHTALSDSASNSSVRYTEACTSSRQSASIGPEKSSNRMTHTGTKTLIQRQSSSEPPSLSPSCSQHSAMTQSLYSFQQPQLGVMHQLKEQLEERTRILQADIKTQQQELHDIKEKLQLANLQMLLQQPIQGEFAGSGPQQQGPGRPAQQNTHPKPQHCVSHSSHTLQPNSSSTQGQQRLVQAGHMQAVSVPMQTHTSLTTPFYSNPMMFSPNHGHRSQHDTHCQRNTHADYSQDGQLRMLLNQPMQTLVQDSNGGPQSSQCTSTIQQTKYPLEQQMMAPSFPVQQVNCNAVLVPSPVFTSPIMIPHNSFITHQTTPTYTTHPPVAPHSLPLQQPQQFFQMQPQGLIHGTPTQALFHTPSVPQQSTVGYVQPQQQQQQQQHHHHSQSGNLSDYRNMLPR

Target ORF information:

RefSeq Version	XM_005165121.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neuronal PAS domain protein 2 (npas2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165121.4

ORF Insert Sequence:

ATGGGAGAAG GAGTGAGCAT GGACAACCTT TCGGAGTTTG GAGGCCTTTG TCCGTCCAGC 
CGGAGGGAAT GGGACACAAG CAGTTGTGTG GATGATTTAA TGGACGAAGA TGAGAAGGAC 
AGAGCAAAAA GGGCGTCCCG GAACAAATCG GAGAAGAAGA GGCGAGACCA GTTCAATGTT 
CTCATTAAGG AGCTGTGTAC AATGCTGCAG GGTCAAGGTC ACCCCCGCAA GATGGACAAA 
TCCACCATCC TGCAGAGGAC CATCGACTTC CTGCAAAAAC AGAAAGAGAT CACGGCACAA 
ACAGAGTCTT GTGAGGTCCG GCAGGACTGG AAGCCTTCTT TCCTCAGCAA CGAGGAGTTC 
ACCCAGTTAA TGCTGGAGGC GTTGGATGGT TTTCTAATAG CTCTTACCAC AGATGGGAAC 
ATCATATATG TATCTGACAG CGTCTCGTCT CTCATTGGCC ATCTGCCGTC AGATATGGTG 
GACCAGAACA TCCTGAACTT CCTGCCAGAG CGAGAGCACG CAGATGTGTA TAAACTCCTG 
TCTTCACACA TGCTTCTGAC TGAGTCTTCT ACTGTAGACC TGCTCAACAG CAATGAGACA 
CACGTCGAGT TCTGCTGTCA TATAGCGAGG GGAAACATTG ACCCGAAAGA ACCGCCTACG 
TACGAATATG TCAAATTTGT TGGAGATTTC AAGTTTCATA ATAATGTGCC TCTGTCTTCA 
TGTAATGGTT ATGATCTGGC GTTTCCTCGC ACGCTGCAGT CCTCAATAGA GGAGCAGGTC 
TGCCTCGTGG CCACAGTCAG ACTCGCAACC CCTCAGTTCC TCAAGGATCT TTGCAACGTG 
GAGGATGTGT GTGATGAATT CACATCAAGA CACAGTCTGG AATGGAAATT TCTCTTCTTA 
GATCACAGGG CCTCTCCTAT CATCGGGTAC CTGCCGTTTG AGGTTTTGGG GACCTCGGGT 
TATGATTACT ACCATGTGGA CGACCTGGAG CTGATTGCTC AATGTCATAA ACAATTGATG 
CAGTGTGGGA AAGGAAAGTC TTGTTACTAT CGGTTCCTGA CTAAAGGACA GCAGTGGATC 
TGGCTGCAGA CGCACTATTA CATCACGTAC CACCAGTGGA ACTCCAAACC TGAGTTCATC 
GTCTGCACAC ACAGCGTGGT CAGTTATGCT GAAGTTCGAG CGGAGAGAAG GAGAGAGTTT 
GGATTGGAGG AGAGCTCATC TGATATGGCC ACATCCTCAA TCAAAGGCCA GGAGGTGTTT 
TTGGACATGT GTCCTCCTCT GGAAGCTACA CGGGACAGAA TCAACAGCGC TCGATCAGTG 
TCATCACAGA GCTCCAGAAA ATCATCACAC ACAGCGCTCT CCGACTCCGC ATCGAACTCC 
TCAGTGAGAT ACACAGAAGC ATGTACGTCA TCCCGGCAGT CTGCTTCCAT CGGCCCTGAG 
AAGAGCTCCA ACAGAATGAC ACACACTGGA ACAAAGACCC TGATTCAGAG ACAGAGCTCT 
TCAGAGCCCC CGTCACTCTC TCCGTCCTGC AGTCAGCATT CAGCCATGAC TCAGTCCCTG 
TACAGCTTCC AGCAGCCTCA GTTAGGTGTC ATGCATCAGC TAAAGGAGCA GCTGGAGGAG 
CGAACGCGGA TCCTACAGGC CGACATCAAA ACACAACAGC AAGAGCTGCA CGACATTAAA 
GAGAAGCTGC AGCTCGCCAA CCTACAGATG TTACTCCAGC AGCCGATACA GGGAGAGTTT 
GCTGGTTCTG GTCCACAGCA GCAGGGTCCA GGCAGGCCGG CTCAACAAAA CACACACCCT 
AAACCTCAAC ACTGTGTTTC ACATTCATCA CACACACTAC AACCCAACAG CTCATCCACT 
CAGGGGCAGC AGAGGCTGGT TCAGGCTGGA CACATGCAGG CCGTCAGTGT TCCAATGCAG 
ACACACACCA GTCTGACCAC ACCCTTCTAC AGCAACCCCA TGATGTTCTC CCCCAACCAC 
GGACACCGAA GCCAACATGA CACACACTGC CAAAGAAACA CACACGCCGA CTACAGCCAG 
GACGGACAGC TACGGATGCT GCTAAACCAG CCAATGCAAA CACTTGTACA AGACAGCAAT 
GGGGGGCCCC AGTCTTCCCA ATGCACCTCA ACTATTCAAC AAACCAAATA TCCTCTGGAG 
CAGCAGATGA TGGCTCCTTC CTTCCCTGTG CAGCAGGTGA ACTGTAACGC TGTGCTAGTC 
CCCTCCCCCG TCTTCACCTC ACCAATCATG ATCCCCCATA ATAGTTTCAT CACCCATCAG 
ACCACCCCCA CCTACACGAC TCACCCGCCG GTTGCCCCAC ACAGCTTACC ACTACAGCAG 
CCTCAGCAGT TCTTTCAGAT GCAGCCTCAG GGTCTGATTC ACGGCACTCC CACTCAGGCC 
TTATTCCACA CCCCTAGTGT TCCACAGCAA AGCACTGTGG GATACGTACA GCCACAACAG 
CAGCAGCAGC AACAGCAGCA TCATCATCAC TCCCAGTCTG GCAACCTGTC GGACTACAGA 
AACATGCTCC CTCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

npas2 ( XM_021476023.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021331698.1 Danio rerio neuronal PAS domain protein 2 (npas2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa10247
Clone ID Related Accession (Same CDS sequence)	XM_005165121.4 , XM_021476023.1 , XM_005165120.3 , XM_009301290.2 , XM_005165121.3 , XM_005165120.4
Accession Version	XM_021476023.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	circadian clock protein PASD1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGAAG GAGTGAGCAT GGACAACCTT TCGGAGTTTG GAGGCCTTTG TCCGTCCAGC 
CGGAGGGAAT GGGACACAAG CAGTTGTGTG GATGATTTAA TGGACGAAGA TGAGAAGGAC 
AGAGCAAAAA GGGCGTCCCG GAACAAATCG GAGAAGAAGA GGCGAGACCA GTTCAATGTT 
CTCATTAAGG AGCTGTGTAC AATGCTGCAG GGTCAAGGTC ACCCCCGCAA GATGGACAAA 
TCCACCATCC TGCAGAGGAC CATCGACTTC CTGCAAAAAC AGAAAGAGAT CACGGCACAA 
ACAGAGTCTT GTGAGGTCCG GCAGGACTGG AAGCCTTCTT TCCTCAGCAA CGAGGAGTTC 
ACCCAGTTAA TGCTGGAGGC GTTGGATGGT TTTCTAATAG CTCTTACCAC AGATGGGAAC 
ATCATATATG TATCTGACAG CGTCTCGTCT CTCATTGGCC ATCTGCCGTC AGATATGGTG 
GACCAGAACA TCCTGAACTT CCTGCCAGAG CGAGAGCACG CAGATGTGTA TAAACTCCTG 
TCTTCACACA TGCTTCTGAC TGAGTCTTCT ACTGTAGACC TGCTCAACAG CAATGAGACA 
CACGTCGAGT TCTGCTGTCA TATAGCGAGG GGAAACATTG ACCCGAAAGA ACCGCCTACG 
TACGAATATG TCAAATTTGT TGGAGATTTC AAGTTTCATA ATAATGTGCC TCTGTCTTCA 
TGTAATGGTT ATGATCTGGC GTTTCCTCGC ACGCTGCAGT CCTCAATAGA GGAGCAGGTC 
TGCCTCGTGG CCACAGTCAG ACTCGCAACC CCTCAGTTCC TCAAGGATCT TTGCAACGTG 
GAGGATGTGT GTGATGAATT CACATCAAGA CACAGTCTGG AATGGAAATT TCTCTTCTTA 
GATCACAGGG CCTCTCCTAT CATCGGGTAC CTGCCGTTTG AGGTTTTGGG GACCTCGGGT 
TATGATTACT ACCATGTGGA CGACCTGGAG CTGATTGCTC AATGTCATAA ACAATTGATG 
CAGTGTGGGA AAGGAAAGTC TTGTTACTAT CGGTTCCTGA CTAAAGGACA GCAGTGGATC 
TGGCTGCAGA CGCACTATTA CATCACGTAC CACCAGTGGA ACTCCAAACC TGAGTTCATC 
GTCTGCACAC ACAGCGTGGT CAGTTATGCT GAAGTTCGAG CGGAGAGAAG GAGAGAGTTT 
GGATTGGAGG AGAGCTCATC TGATATGGCC ACATCCTCAA TCAAAGGCCA GGAGGTGTTT 
TTGGACATGT GTCCTCCTCT GGAAGCTACA CGGGACAGAA TCAACAGCGC TCGATCAGTG 
TCATCACAGA GCTCCAGAAA ATCATCACAC ACAGCGCTCT CCGACTCCGC ATCGAACTCC 
TCAGTGAGAT ACACAGAAGC ATGTACGTCA TCCCGGCAGT CTGCTTCCAT CGGCCCTGAG 
AAGAGCTCCA ACAGAATGAC ACACACTGGA ACAAAGACCC TGATTCAGAG ACAGAGCTCT 
TCAGAGCCCC CGTCACTCTC TCCGTCCTGC AGTCAGCATT CAGCCATGAC TCAGTCCCTG 
TACAGCTTCC AGCAGCCTCA GTTAGGTGTC ATGCATCAGC TAAAGGAGCA GCTGGAGGAG 
CGAACGCGGA TCCTACAGGC CGACATCAAA ACACAACAGC AAGAGCTGCA CGACATTAAA 
GAGAAGCTGC AGCTCGCCAA CCTACAGATG TTACTCCAGC AGCCGATACA GGGAGAGTTT 
GCTGGTTCTG GTCCACAGCA GCAGGGTCCA GGCAGGCCGG CTCAACAAAA CACACACCCT 
AAACCTCAAC ACTGTGTTTC ACATTCATCA CACACACTAC AACCCAACAG CTCATCCACT 
CAGGGGCAGC AGAGGCTGGT TCAGGCTGGA CACATGCAGG CCGTCAGTGT TCCAATGCAG 
ACACACACCA GTCTGACCAC ACCCTTCTAC AGCAACCCCA TGATGTTCTC CCCCAACCAC 
GGACACCGAA GCCAACATGA CACACACTGC CAAAGAAACA CACACGCCGA CTACAGCCAG 
GACGGACAGC TACGGATGCT GCTAAACCAG CCAATGCAAA CACTTGTACA AGACAGCAAT 
GGGGGGCCCC AGTCTTCCCA ATGCACCTCA ACTATTCAAC AAACCAAATA TCCTCTGGAG 
CAGCAGATGA TGGCTCCTTC CTTCCCTGTG CAGCAGGTGA ACTGTAACGC TGTGCTAGTC 
CCCTCCCCCG TCTTCACCTC ACCAATCATG ATCCCCCATA ATAGTTTCAT CACCCATCAG 
ACCACCCCCA CCTACACGAC TCACCCGCCG GTTGCCCCAC ACAGCTTACC ACTACAGCAG 
CCTCAGCAGT TCTTTCAGAT GCAGCCTCAG GGTCTGATTC ACGGCACTCC CACTCAGGCC 
TTATTCCACA CCCCTAGTGT TCCACAGCAA AGCACTGTGG GATACGTACA GCCACAACAG 
CAGCAGCAGC AACAGCAGCA TCATCATCAC TCCCAGTCTG GCAACCTGTC GGACTACAGA 
AACATGCTCC CTCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021331698.1
CDS	471..3008
Translation	MGEGVSMDNLSEFGGLCPSSRREWDTSSCVDDLMDEDEKDRAKRASRNKSEKKRRDQFNVLIKELCTMLQGQGHPRKMDKSTILQRTIDFLQKQKEITAQTESCEVRQDWKPSFLSNEEFTQLMLEALDGFLIALTTDGNIIYVSDSVSSLIGHLPSDMVDQNILNFLPEREHADVYKLLSSHMLLTESSTVDLLNSNETHVEFCCHIARGNIDPKEPPTYEYVKFVGDFKFHNNVPLSSCNGYDLAFPRTLQSSIEEQVCLVATVRLATPQFLKDLCNVEDVCDEFTSRHSLEWKFLFLDHRASPIIGYLPFEVLGTSGYDYYHVDDLELIAQCHKQLMQCGKGKSCYYRFLTKGQQWIWLQTHYYITYHQWNSKPEFIVCTHSVVSYAEVRAERRREFGLEESSSDMATSSIKGQEVFLDMCPPLEATRDRINSARSVSSQSSRKSSHTALSDSASNSSVRYTEACTSSRQSASIGPEKSSNRMTHTGTKTLIQRQSSSEPPSLSPSCSQHSAMTQSLYSFQQPQLGVMHQLKEQLEERTRILQADIKTQQQELHDIKEKLQLANLQMLLQQPIQGEFAGSGPQQQGPGRPAQQNTHPKPQHCVSHSSHTLQPNSSSTQGQQRLVQAGHMQAVSVPMQTHTSLTTPFYSNPMMFSPNHGHRSQHDTHCQRNTHADYSQDGQLRMLLNQPMQTLVQDSNGGPQSSQCTSTIQQTKYPLEQQMMAPSFPVQQVNCNAVLVPSPVFTSPIMIPHNSFITHQTTPTYTTHPPVAPHSLPLQQPQQFFQMQPQGLIHGTPTQALFHTPSVPQQSTVGYVQPQQQQQQQQHHHHSQSGNLSDYRNMLPR

Target ORF information:

RefSeq Version	XM_021476023.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neuronal PAS domain protein 2 (npas2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021476023.1

ORF Insert Sequence:

ATGGGAGAAG GAGTGAGCAT GGACAACCTT TCGGAGTTTG GAGGCCTTTG TCCGTCCAGC 
CGGAGGGAAT GGGACACAAG CAGTTGTGTG GATGATTTAA TGGACGAAGA TGAGAAGGAC 
AGAGCAAAAA GGGCGTCCCG GAACAAATCG GAGAAGAAGA GGCGAGACCA GTTCAATGTT 
CTCATTAAGG AGCTGTGTAC AATGCTGCAG GGTCAAGGTC ACCCCCGCAA GATGGACAAA 
TCCACCATCC TGCAGAGGAC CATCGACTTC CTGCAAAAAC AGAAAGAGAT CACGGCACAA 
ACAGAGTCTT GTGAGGTCCG GCAGGACTGG AAGCCTTCTT TCCTCAGCAA CGAGGAGTTC 
ACCCAGTTAA TGCTGGAGGC GTTGGATGGT TTTCTAATAG CTCTTACCAC AGATGGGAAC 
ATCATATATG TATCTGACAG CGTCTCGTCT CTCATTGGCC ATCTGCCGTC AGATATGGTG 
GACCAGAACA TCCTGAACTT CCTGCCAGAG CGAGAGCACG CAGATGTGTA TAAACTCCTG 
TCTTCACACA TGCTTCTGAC TGAGTCTTCT ACTGTAGACC TGCTCAACAG CAATGAGACA 
CACGTCGAGT TCTGCTGTCA TATAGCGAGG GGAAACATTG ACCCGAAAGA ACCGCCTACG 
TACGAATATG TCAAATTTGT TGGAGATTTC AAGTTTCATA ATAATGTGCC TCTGTCTTCA 
TGTAATGGTT ATGATCTGGC GTTTCCTCGC ACGCTGCAGT CCTCAATAGA GGAGCAGGTC 
TGCCTCGTGG CCACAGTCAG ACTCGCAACC CCTCAGTTCC TCAAGGATCT TTGCAACGTG 
GAGGATGTGT GTGATGAATT CACATCAAGA CACAGTCTGG AATGGAAATT TCTCTTCTTA 
GATCACAGGG CCTCTCCTAT CATCGGGTAC CTGCCGTTTG AGGTTTTGGG GACCTCGGGT 
TATGATTACT ACCATGTGGA CGACCTGGAG CTGATTGCTC AATGTCATAA ACAATTGATG 
CAGTGTGGGA AAGGAAAGTC TTGTTACTAT CGGTTCCTGA CTAAAGGACA GCAGTGGATC 
TGGCTGCAGA CGCACTATTA CATCACGTAC CACCAGTGGA ACTCCAAACC TGAGTTCATC 
GTCTGCACAC ACAGCGTGGT CAGTTATGCT GAAGTTCGAG CGGAGAGAAG GAGAGAGTTT 
GGATTGGAGG AGAGCTCATC TGATATGGCC ACATCCTCAA TCAAAGGCCA GGAGGTGTTT 
TTGGACATGT GTCCTCCTCT GGAAGCTACA CGGGACAGAA TCAACAGCGC TCGATCAGTG 
TCATCACAGA GCTCCAGAAA ATCATCACAC ACAGCGCTCT CCGACTCCGC ATCGAACTCC 
TCAGTGAGAT ACACAGAAGC ATGTACGTCA TCCCGGCAGT CTGCTTCCAT CGGCCCTGAG 
AAGAGCTCCA ACAGAATGAC ACACACTGGA ACAAAGACCC TGATTCAGAG ACAGAGCTCT 
TCAGAGCCCC CGTCACTCTC TCCGTCCTGC AGTCAGCATT CAGCCATGAC TCAGTCCCTG 
TACAGCTTCC AGCAGCCTCA GTTAGGTGTC ATGCATCAGC TAAAGGAGCA GCTGGAGGAG 
CGAACGCGGA TCCTACAGGC CGACATCAAA ACACAACAGC AAGAGCTGCA CGACATTAAA 
GAGAAGCTGC AGCTCGCCAA CCTACAGATG TTACTCCAGC AGCCGATACA GGGAGAGTTT 
GCTGGTTCTG GTCCACAGCA GCAGGGTCCA GGCAGGCCGG CTCAACAAAA CACACACCCT 
AAACCTCAAC ACTGTGTTTC ACATTCATCA CACACACTAC AACCCAACAG CTCATCCACT 
CAGGGGCAGC AGAGGCTGGT TCAGGCTGGA CACATGCAGG CCGTCAGTGT TCCAATGCAG 
ACACACACCA GTCTGACCAC ACCCTTCTAC AGCAACCCCA TGATGTTCTC CCCCAACCAC 
GGACACCGAA GCCAACATGA CACACACTGC CAAAGAAACA CACACGCCGA CTACAGCCAG 
GACGGACAGC TACGGATGCT GCTAAACCAG CCAATGCAAA CACTTGTACA AGACAGCAAT 
GGGGGGCCCC AGTCTTCCCA ATGCACCTCA ACTATTCAAC AAACCAAATA TCCTCTGGAG 
CAGCAGATGA TGGCTCCTTC CTTCCCTGTG CAGCAGGTGA ACTGTAACGC TGTGCTAGTC 
CCCTCCCCCG TCTTCACCTC ACCAATCATG ATCCCCCATA ATAGTTTCAT CACCCATCAG 
ACCACCCCCA CCTACACGAC TCACCCGCCG GTTGCCCCAC ACAGCTTACC ACTACAGCAG 
CCTCAGCAGT TCTTTCAGAT GCAGCCTCAG GGTCTGATTC ACGGCACTCC CACTCAGGCC 
TTATTCCACA CCCCTAGTGT TCCACAGCAA AGCACTGTGG GATACGTACA GCCACAACAG 
CAGCAGCAGC AACAGCAGCA TCATCATCAC TCCCAGTCTG GCAACCTGTC GGACTACAGA 
AACATGCTCC CTCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

npas2 ( XM_005165120.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165177.1 Danio rerio neuronal PAS domain protein 2 (npas2), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa10247
Clone ID Related Accession (Same CDS sequence)	XM_005165121.4 , XM_021476023.1 , XM_005165120.3 , XM_009301290.2 , XM_005165121.3 , XM_005165120.4
Accession Version	XM_005165120.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	circadian clock protein PASD1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165120.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGAAG GAGTGAGCAT GGACAACCTT TCGGAGTTTG GAGGCCTTTG TCCGTCCAGC 
CGGAGGGAAT GGGACACAAG CAGTTGTGTG GATGATTTAA TGGACGAAGA TGAGAAGGAC 
AGAGCAAAAA GGGCGTCCCG GAACAAATCG GAGAAGAAGA GGCGAGACCA GTTCAATGTT 
CTCATTAAGG AGCTGTGTAC AATGCTGCAG GGTCAAGGTC ACCCCCGCAA GATGGACAAA 
TCCACCATCC TGCAGAGGAC CATCGACTTC CTGCAAAAAC AGAAAGAGAT CACGGCACAA 
ACAGAGTCTT GTGAGGTCCG GCAGGACTGG AAGCCTTCTT TCCTCAGCAA CGAGGAGTTC 
ACCCAGTTAA TGCTGGAGGC GTTGGATGGT TTTCTAATAG CTCTTACCAC AGATGGGAAC 
ATCATATATG TATCTGACAG CGTCTCGTCT CTCATTGGCC ATCTGCCGTC AGATATGGTG 
GACCAGAACA TCCTGAACTT CCTGCCAGAG CGAGAGCACG CAGATGTGTA TAAACTCCTG 
TCTTCACACA TGCTTCTGAC TGAGTCTTCT ACTGTAGACC TGCTCAACAG CAATGAGACA 
CACGTCGAGT TCTGCTGTCA TATAGCGAGG GGAAACATTG ACCCGAAAGA ACCGCCTACG 
TACGAATATG TCAAATTTGT TGGAGATTTC AAGTTTCATA ATAATGTGCC TCTGTCTTCA 
TGTAATGGTT ATGATCTGGC GTTTCCTCGC ACGCTGCAGT CCTCAATAGA GGAGCAGGTC 
TGCCTCGTGG CCACAGTCAG ACTCGCAACC CCTCAGTTCC TCAAGGATCT TTGCAACGTG 
GAGGATGTGT GTGATGAATT CACATCAAGA CACAGTCTGG AATGGAAATT TCTCTTCTTA 
GATCACAGGG CCTCTCCTAT CATCGGGTAC CTGCCGTTTG AGGTTTTGGG GACCTCGGGT 
TATGATTACT ACCATGTGGA CGACCTGGAG CTGATTGCTC AATGTCATAA ACAATTGATG 
CAGTGTGGGA AAGGAAAGTC TTGTTACTAT CGGTTCCTGA CTAAAGGACA GCAGTGGATC 
TGGCTGCAGA CGCACTATTA CATCACGTAC CACCAGTGGA ACTCCAAACC TGAGTTCATC 
GTCTGCACAC ACAGCGTGGT CAGTTATGCT GAAGTTCGAG CGGAGAGAAG GAGAGAGTTT 
GGATTGGAGG AGAGCTCATC TGATATGGCC ACATCCTCAA TCAAAGGCCA GGAGGTGTTT 
TTGGACATGT GTCCTCCTCT GGAAGCTACA CGGGACAGAA TCAACAGCGC TCGATCAGTG 
TCATCACAGA GCTCCAGAAA ATCATCACAC ACAGCGCTCT CCGACTCCGC ATCGAACTCC 
TCAGTGAGAT ACACAGAAGC ATGTACGTCA TCCCGGCAGT CTGCTTCCAT CGGCCCTGAG 
AAGAGCTCCA ACAGAATGAC ACACACTGGA ACAAAGACCC TGATTCAGAG ACAGAGCTCT 
TCAGAGCCCC CGTCACTCTC TCCGTCCTGC AGTCAGCATT CAGCCATGAC TCAGTCCCTG 
TACAGCTTCC AGCAGCCTCA GTTAGGTGTC ATGCATCAGC TAAAGGAGCA GCTGGAGGAG 
CGAACGCGGA TCCTACAGGC CGACATCAAA ACACAACAGC AAGAGCTGCA CGACATTAAA 
GAGAAGCTGC AGCTCGCCAA CCTACAGATG TTACTCCAGC AGCCGATACA GGGAGAGTTT 
GCTGGTTCTG GTCCACAGCA GCAGGGTCCA GGCAGGCCGG CTCAACAAAA CACACACCCT 
AAACCTCAAC ACTGTGTTTC ACATTCATCA CACACACTAC AACCCAACAG CTCATCCACT 
CAGGGGCAGC AGAGGCTGGT TCAGGCTGGA CACATGCAGG CCGTCAGTGT TCCAATGCAG 
ACACACACCA GTCTGACCAC ACCCTTCTAC AGCAACCCCA TGATGTTCTC CCCCAACCAC 
GGACACCGAA GCCAACATGA CACACACTGC CAAAGAAACA CACACGCCGA CTACAGCCAG 
GACGGACAGC TACGGATGCT GCTAAACCAG CCAATGCAAA CACTTGTACA AGACAGCAAT 
GGGGGGCCCC AGTCTTCCCA ATGCACCTCA ACTATTCAAC AAACCAAATA TCCTCTGGAG 
CAGCAGATGA TGGCTCCTTC CTTCCCTGTG CAGCAGGTGA ACTGTAACGC TGTGCTAGTC 
CCCTCCCCCG TCTTCACCTC ACCAATCATG ATCCCCCATA ATAGTTTCAT CACCCATCAG 
ACCACCCCCA CCTACACGAC TCACCCGCCG GTTGCCCCAC ACAGCTTACC ACTACAGCAG 
CCTCAGCAGT TCTTTCAGAT GCAGCCTCAG GGTCTGATTC ACGGCACTCC CACTCAGGCC 
TTATTCCACA CCCCTAGTGT TCCACAGCAA AGCACTGTGG GATACGTACA GCCACAACAG 
CAGCAGCAGC AACAGCAGCA TCATCATCAC TCCCAGTCTG GCAACCTGTC GGACTACAGA 
AACATGCTCC CTCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165177.1
CDS	535..3072
Translation	MGEGVSMDNLSEFGGLCPSSRREWDTSSCVDDLMDEDEKDRAKRASRNKSEKKRRDQFNVLIKELCTMLQGQGHPRKMDKSTILQRTIDFLQKQKEITAQTESCEVRQDWKPSFLSNEEFTQLMLEALDGFLIALTTDGNIIYVSDSVSSLIGHLPSDMVDQNILNFLPEREHADVYKLLSSHMLLTESSTVDLLNSNETHVEFCCHIARGNIDPKEPPTYEYVKFVGDFKFHNNVPLSSCNGYDLAFPRTLQSSIEEQVCLVATVRLATPQFLKDLCNVEDVCDEFTSRHSLEWKFLFLDHRASPIIGYLPFEVLGTSGYDYYHVDDLELIAQCHKQLMQCGKGKSCYYRFLTKGQQWIWLQTHYYITYHQWNSKPEFIVCTHSVVSYAEVRAERRREFGLEESSSDMATSSIKGQEVFLDMCPPLEATRDRINSARSVSSQSSRKSSHTALSDSASNSSVRYTEACTSSRQSASIGPEKSSNRMTHTGTKTLIQRQSSSEPPSLSPSCSQHSAMTQSLYSFQQPQLGVMHQLKEQLEERTRILQADIKTQQQELHDIKEKLQLANLQMLLQQPIQGEFAGSGPQQQGPGRPAQQNTHPKPQHCVSHSSHTLQPNSSSTQGQQRLVQAGHMQAVSVPMQTHTSLTTPFYSNPMMFSPNHGHRSQHDTHCQRNTHADYSQDGQLRMLLNQPMQTLVQDSNGGPQSSQCTSTIQQTKYPLEQQMMAPSFPVQQVNCNAVLVPSPVFTSPIMIPHNSFITHQTTPTYTTHPPVAPHSLPLQQPQQFFQMQPQGLIHGTPTQALFHTPSVPQQSTVGYVQPQQQQQQQQHHHHSQSGNLSDYRNMLPR

Target ORF information:

RefSeq Version	XM_005165120.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neuronal PAS domain protein 2 (npas2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165120.3

ORF Insert Sequence:

ATGGGAGAAG GAGTGAGCAT GGACAACCTT TCGGAGTTTG GAGGCCTTTG TCCGTCCAGC 
CGGAGGGAAT GGGACACAAG CAGTTGTGTG GATGATTTAA TGGACGAAGA TGAGAAGGAC 
AGAGCAAAAA GGGCGTCCCG GAACAAATCG GAGAAGAAGA GGCGAGACCA GTTCAATGTT 
CTCATTAAGG AGCTGTGTAC AATGCTGCAG GGTCAAGGTC ACCCCCGCAA GATGGACAAA 
TCCACCATCC TGCAGAGGAC CATCGACTTC CTGCAAAAAC AGAAAGAGAT CACGGCACAA 
ACAGAGTCTT GTGAGGTCCG GCAGGACTGG AAGCCTTCTT TCCTCAGCAA CGAGGAGTTC 
ACCCAGTTAA TGCTGGAGGC GTTGGATGGT TTTCTAATAG CTCTTACCAC AGATGGGAAC 
ATCATATATG TATCTGACAG CGTCTCGTCT CTCATTGGCC ATCTGCCGTC AGATATGGTG 
GACCAGAACA TCCTGAACTT CCTGCCAGAG CGAGAGCACG CAGATGTGTA TAAACTCCTG 
TCTTCACACA TGCTTCTGAC TGAGTCTTCT ACTGTAGACC TGCTCAACAG CAATGAGACA 
CACGTCGAGT TCTGCTGTCA TATAGCGAGG GGAAACATTG ACCCGAAAGA ACCGCCTACG 
TACGAATATG TCAAATTTGT TGGAGATTTC AAGTTTCATA ATAATGTGCC TCTGTCTTCA 
TGTAATGGTT ATGATCTGGC GTTTCCTCGC ACGCTGCAGT CCTCAATAGA GGAGCAGGTC 
TGCCTCGTGG CCACAGTCAG ACTCGCAACC CCTCAGTTCC TCAAGGATCT TTGCAACGTG 
GAGGATGTGT GTGATGAATT CACATCAAGA CACAGTCTGG AATGGAAATT TCTCTTCTTA 
GATCACAGGG CCTCTCCTAT CATCGGGTAC CTGCCGTTTG AGGTTTTGGG GACCTCGGGT 
TATGATTACT ACCATGTGGA CGACCTGGAG CTGATTGCTC AATGTCATAA ACAATTGATG 
CAGTGTGGGA AAGGAAAGTC TTGTTACTAT CGGTTCCTGA CTAAAGGACA GCAGTGGATC 
TGGCTGCAGA CGCACTATTA CATCACGTAC CACCAGTGGA ACTCCAAACC TGAGTTCATC 
GTCTGCACAC ACAGCGTGGT CAGTTATGCT GAAGTTCGAG CGGAGAGAAG GAGAGAGTTT 
GGATTGGAGG AGAGCTCATC TGATATGGCC ACATCCTCAA TCAAAGGCCA GGAGGTGTTT 
TTGGACATGT GTCCTCCTCT GGAAGCTACA CGGGACAGAA TCAACAGCGC TCGATCAGTG 
TCATCACAGA GCTCCAGAAA ATCATCACAC ACAGCGCTCT CCGACTCCGC ATCGAACTCC 
TCAGTGAGAT ACACAGAAGC ATGTACGTCA TCCCGGCAGT CTGCTTCCAT CGGCCCTGAG 
AAGAGCTCCA ACAGAATGAC ACACACTGGA ACAAAGACCC TGATTCAGAG ACAGAGCTCT 
TCAGAGCCCC CGTCACTCTC TCCGTCCTGC AGTCAGCATT CAGCCATGAC TCAGTCCCTG 
TACAGCTTCC AGCAGCCTCA GTTAGGTGTC ATGCATCAGC TAAAGGAGCA GCTGGAGGAG 
CGAACGCGGA TCCTACAGGC CGACATCAAA ACACAACAGC AAGAGCTGCA CGACATTAAA 
GAGAAGCTGC AGCTCGCCAA CCTACAGATG TTACTCCAGC AGCCGATACA GGGAGAGTTT 
GCTGGTTCTG GTCCACAGCA GCAGGGTCCA GGCAGGCCGG CTCAACAAAA CACACACCCT 
AAACCTCAAC ACTGTGTTTC ACATTCATCA CACACACTAC AACCCAACAG CTCATCCACT 
CAGGGGCAGC AGAGGCTGGT TCAGGCTGGA CACATGCAGG CCGTCAGTGT TCCAATGCAG 
ACACACACCA GTCTGACCAC ACCCTTCTAC AGCAACCCCA TGATGTTCTC CCCCAACCAC 
GGACACCGAA GCCAACATGA CACACACTGC CAAAGAAACA CACACGCCGA CTACAGCCAG 
GACGGACAGC TACGGATGCT GCTAAACCAG CCAATGCAAA CACTTGTACA AGACAGCAAT 
GGGGGGCCCC AGTCTTCCCA ATGCACCTCA ACTATTCAAC AAACCAAATA TCCTCTGGAG 
CAGCAGATGA TGGCTCCTTC CTTCCCTGTG CAGCAGGTGA ACTGTAACGC TGTGCTAGTC 
CCCTCCCCCG TCTTCACCTC ACCAATCATG ATCCCCCATA ATAGTTTCAT CACCCATCAG 
ACCACCCCCA CCTACACGAC TCACCCGCCG GTTGCCCCAC ACAGCTTACC ACTACAGCAG 
CCTCAGCAGT TCTTTCAGAT GCAGCCTCAG GGTCTGATTC ACGGCACTCC CACTCAGGCC 
TTATTCCACA CCCCTAGTGT TCCACAGCAA AGCACTGTGG GATACGTACA GCCACAACAG 
CAGCAGCAGC AACAGCAGCA TCATCATCAC TCCCAGTCTG GCAACCTGTC GGACTACAGA 
AACATGCTCC CTCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

npas2 ( XM_009301290.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009299565.1 Danio rerio neuronal PAS domain protein 2 (npas2), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa10247
Clone ID Related Accession (Same CDS sequence)	XM_005165121.4 , XM_021476023.1 , XM_005165120.3 , XM_009301290.2 , XM_005165121.3 , XM_005165120.4
Accession Version	XM_009301290.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	circadian clock protein PASD1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009301290.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGAAG GAGTGAGCAT GGACAACCTT TCGGAGTTTG GAGGCCTTTG TCCGTCCAGC 
CGGAGGGAAT GGGACACAAG CAGTTGTGTG GATGATTTAA TGGACGAAGA TGAGAAGGAC 
AGAGCAAAAA GGGCGTCCCG GAACAAATCG GAGAAGAAGA GGCGAGACCA GTTCAATGTT 
CTCATTAAGG AGCTGTGTAC AATGCTGCAG GGTCAAGGTC ACCCCCGCAA GATGGACAAA 
TCCACCATCC TGCAGAGGAC CATCGACTTC CTGCAAAAAC AGAAAGAGAT CACGGCACAA 
ACAGAGTCTT GTGAGGTCCG GCAGGACTGG AAGCCTTCTT TCCTCAGCAA CGAGGAGTTC 
ACCCAGTTAA TGCTGGAGGC GTTGGATGGT TTTCTAATAG CTCTTACCAC AGATGGGAAC 
ATCATATATG TATCTGACAG CGTCTCGTCT CTCATTGGCC ATCTGCCGTC AGATATGGTG 
GACCAGAACA TCCTGAACTT CCTGCCAGAG CGAGAGCACG CAGATGTGTA TAAACTCCTG 
TCTTCACACA TGCTTCTGAC TGAGTCTTCT ACTGTAGACC TGCTCAACAG CAATGAGACA 
CACGTCGAGT TCTGCTGTCA TATAGCGAGG GGAAACATTG ACCCGAAAGA ACCGCCTACG 
TACGAATATG TCAAATTTGT TGGAGATTTC AAGTTTCATA ATAATGTGCC TCTGTCTTCA 
TGTAATGGTT ATGATCTGGC GTTTCCTCGC ACGCTGCAGT CCTCAATAGA GGAGCAGGTC 
TGCCTCGTGG CCACAGTCAG ACTCGCAACC CCTCAGTTCC TCAAGGATCT TTGCAACGTG 
GAGGATGTGT GTGATGAATT CACATCAAGA CACAGTCTGG AATGGAAATT TCTCTTCTTA 
GATCACAGGG CCTCTCCTAT CATCGGGTAC CTGCCGTTTG AGGTTTTGGG GACCTCGGGT 
TATGATTACT ACCATGTGGA CGACCTGGAG CTGATTGCTC AATGTCATAA ACAATTGATG 
CAGTGTGGGA AAGGAAAGTC TTGTTACTAT CGGTTCCTGA CTAAAGGACA GCAGTGGATC 
TGGCTGCAGA CGCACTATTA CATCACGTAC CACCAGTGGA ACTCCAAACC TGAGTTCATC 
GTCTGCACAC ACAGCGTGGT CAGTTATGCT GAAGTTCGAG CGGAGAGAAG GAGAGAGTTT 
GGATTGGAGG AGAGCTCATC TGATATGGCC ACATCCTCAA TCAAAGGCCA GGAGGTGTTT 
TTGGACATGT GTCCTCCTCT GGAAGCTACA CGGGACAGAA TCAACAGCGC TCGATCAGTG 
TCATCACAGA GCTCCAGAAA ATCATCACAC ACAGCGCTCT CCGACTCCGC ATCGAACTCC 
TCAGTGAGAT ACACAGAAGC ATGTACGTCA TCCCGGCAGT CTGCTTCCAT CGGCCCTGAG 
AAGAGCTCCA ACAGAATGAC ACACACTGGA ACAAAGACCC TGATTCAGAG ACAGAGCTCT 
TCAGAGCCCC CGTCACTCTC TCCGTCCTGC AGTCAGCATT CAGCCATGAC TCAGTCCCTG 
TACAGCTTCC AGCAGCCTCA GTTAGGTGTC ATGCATCAGC TAAAGGAGCA GCTGGAGGAG 
CGAACGCGGA TCCTACAGGC CGACATCAAA ACACAACAGC AAGAGCTGCA CGACATTAAA 
GAGAAGCTGC AGCTCGCCAA CCTACAGATG TTACTCCAGC AGCCGATACA GGGAGAGTTT 
GCTGGTTCTG GTCCACAGCA GCAGGGTCCA GGCAGGCCGG CTCAACAAAA CACACACCCT 
AAACCTCAAC ACTGTGTTTC ACATTCATCA CACACACTAC AACCCAACAG CTCATCCACT 
CAGGGGCAGC AGAGGCTGGT TCAGGCTGGA CACATGCAGG CCGTCAGTGT TCCAATGCAG 
ACACACACCA GTCTGACCAC ACCCTTCTAC AGCAACCCCA TGATGTTCTC CCCCAACCAC 
GGACACCGAA GCCAACATGA CACACACTGC CAAAGAAACA CACACGCCGA CTACAGCCAG 
GACGGACAGC TACGGATGCT GCTAAACCAG CCAATGCAAA CACTTGTACA AGACAGCAAT 
GGGGGGCCCC AGTCTTCCCA ATGCACCTCA ACTATTCAAC AAACCAAATA TCCTCTGGAG 
CAGCAGATGA TGGCTCCTTC CTTCCCTGTG CAGCAGGTGA ACTGTAACGC TGTGCTAGTC 
CCCTCCCCCG TCTTCACCTC ACCAATCATG ATCCCCCATA ATAGTTTCAT CACCCATCAG 
ACCACCCCCA CCTACACGAC TCACCCGCCG GTTGCCCCAC ACAGCTTACC ACTACAGCAG 
CCTCAGCAGT TCTTTCAGAT GCAGCCTCAG GGTCTGATTC ACGGCACTCC CACTCAGGCC 
TTATTCCACA CCCCTAGTGT TCCACAGCAA AGCACTGTGG GATACGTACA GCCACAACAG 
CAGCAGCAGC AACAGCAGCA TCATCATCAC TCCCAGTCTG GCAACCTGTC GGACTACAGA 
AACATGCTCC CTCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009299565.1
CDS	741..3278
Translation	MGEGVSMDNLSEFGGLCPSSRREWDTSSCVDDLMDEDEKDRAKRASRNKSEKKRRDQFNVLIKELCTMLQGQGHPRKMDKSTILQRTIDFLQKQKEITAQTESCEVRQDWKPSFLSNEEFTQLMLEALDGFLIALTTDGNIIYVSDSVSSLIGHLPSDMVDQNILNFLPEREHADVYKLLSSHMLLTESSTVDLLNSNETHVEFCCHIARGNIDPKEPPTYEYVKFVGDFKFHNNVPLSSCNGYDLAFPRTLQSSIEEQVCLVATVRLATPQFLKDLCNVEDVCDEFTSRHSLEWKFLFLDHRASPIIGYLPFEVLGTSGYDYYHVDDLELIAQCHKQLMQCGKGKSCYYRFLTKGQQWIWLQTHYYITYHQWNSKPEFIVCTHSVVSYAEVRAERRREFGLEESSSDMATSSIKGQEVFLDMCPPLEATRDRINSARSVSSQSSRKSSHTALSDSASNSSVRYTEACTSSRQSASIGPEKSSNRMTHTGTKTLIQRQSSSEPPSLSPSCSQHSAMTQSLYSFQQPQLGVMHQLKEQLEERTRILQADIKTQQQELHDIKEKLQLANLQMLLQQPIQGEFAGSGPQQQGPGRPAQQNTHPKPQHCVSHSSHTLQPNSSSTQGQQRLVQAGHMQAVSVPMQTHTSLTTPFYSNPMMFSPNHGHRSQHDTHCQRNTHADYSQDGQLRMLLNQPMQTLVQDSNGGPQSSQCTSTIQQTKYPLEQQMMAPSFPVQQVNCNAVLVPSPVFTSPIMIPHNSFITHQTTPTYTTHPPVAPHSLPLQQPQQFFQMQPQGLIHGTPTQALFHTPSVPQQSTVGYVQPQQQQQQQQHHHHSQSGNLSDYRNMLPR

Target ORF information:

RefSeq Version	XM_009301290.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neuronal PAS domain protein 2 (npas2), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009301290.2

ORF Insert Sequence:

ATGGGAGAAG GAGTGAGCAT GGACAACCTT TCGGAGTTTG GAGGCCTTTG TCCGTCCAGC 
CGGAGGGAAT GGGACACAAG CAGTTGTGTG GATGATTTAA TGGACGAAGA TGAGAAGGAC 
AGAGCAAAAA GGGCGTCCCG GAACAAATCG GAGAAGAAGA GGCGAGACCA GTTCAATGTT 
CTCATTAAGG AGCTGTGTAC AATGCTGCAG GGTCAAGGTC ACCCCCGCAA GATGGACAAA 
TCCACCATCC TGCAGAGGAC CATCGACTTC CTGCAAAAAC AGAAAGAGAT CACGGCACAA 
ACAGAGTCTT GTGAGGTCCG GCAGGACTGG AAGCCTTCTT TCCTCAGCAA CGAGGAGTTC 
ACCCAGTTAA TGCTGGAGGC GTTGGATGGT TTTCTAATAG CTCTTACCAC AGATGGGAAC 
ATCATATATG TATCTGACAG CGTCTCGTCT CTCATTGGCC ATCTGCCGTC AGATATGGTG 
GACCAGAACA TCCTGAACTT CCTGCCAGAG CGAGAGCACG CAGATGTGTA TAAACTCCTG 
TCTTCACACA TGCTTCTGAC TGAGTCTTCT ACTGTAGACC TGCTCAACAG CAATGAGACA 
CACGTCGAGT TCTGCTGTCA TATAGCGAGG GGAAACATTG ACCCGAAAGA ACCGCCTACG 
TACGAATATG TCAAATTTGT TGGAGATTTC AAGTTTCATA ATAATGTGCC TCTGTCTTCA 
TGTAATGGTT ATGATCTGGC GTTTCCTCGC ACGCTGCAGT CCTCAATAGA GGAGCAGGTC 
TGCCTCGTGG CCACAGTCAG ACTCGCAACC CCTCAGTTCC TCAAGGATCT TTGCAACGTG 
GAGGATGTGT GTGATGAATT CACATCAAGA CACAGTCTGG AATGGAAATT TCTCTTCTTA 
GATCACAGGG CCTCTCCTAT CATCGGGTAC CTGCCGTTTG AGGTTTTGGG GACCTCGGGT 
TATGATTACT ACCATGTGGA CGACCTGGAG CTGATTGCTC AATGTCATAA ACAATTGATG 
CAGTGTGGGA AAGGAAAGTC TTGTTACTAT CGGTTCCTGA CTAAAGGACA GCAGTGGATC 
TGGCTGCAGA CGCACTATTA CATCACGTAC CACCAGTGGA ACTCCAAACC TGAGTTCATC 
GTCTGCACAC ACAGCGTGGT CAGTTATGCT GAAGTTCGAG CGGAGAGAAG GAGAGAGTTT 
GGATTGGAGG AGAGCTCATC TGATATGGCC ACATCCTCAA TCAAAGGCCA GGAGGTGTTT 
TTGGACATGT GTCCTCCTCT GGAAGCTACA CGGGACAGAA TCAACAGCGC TCGATCAGTG 
TCATCACAGA GCTCCAGAAA ATCATCACAC ACAGCGCTCT CCGACTCCGC ATCGAACTCC 
TCAGTGAGAT ACACAGAAGC ATGTACGTCA TCCCGGCAGT CTGCTTCCAT CGGCCCTGAG 
AAGAGCTCCA ACAGAATGAC ACACACTGGA ACAAAGACCC TGATTCAGAG ACAGAGCTCT 
TCAGAGCCCC CGTCACTCTC TCCGTCCTGC AGTCAGCATT CAGCCATGAC TCAGTCCCTG 
TACAGCTTCC AGCAGCCTCA GTTAGGTGTC ATGCATCAGC TAAAGGAGCA GCTGGAGGAG 
CGAACGCGGA TCCTACAGGC CGACATCAAA ACACAACAGC AAGAGCTGCA CGACATTAAA 
GAGAAGCTGC AGCTCGCCAA CCTACAGATG TTACTCCAGC AGCCGATACA GGGAGAGTTT 
GCTGGTTCTG GTCCACAGCA GCAGGGTCCA GGCAGGCCGG CTCAACAAAA CACACACCCT 
AAACCTCAAC ACTGTGTTTC ACATTCATCA CACACACTAC AACCCAACAG CTCATCCACT 
CAGGGGCAGC AGAGGCTGGT TCAGGCTGGA CACATGCAGG CCGTCAGTGT TCCAATGCAG 
ACACACACCA GTCTGACCAC ACCCTTCTAC AGCAACCCCA TGATGTTCTC CCCCAACCAC 
GGACACCGAA GCCAACATGA CACACACTGC CAAAGAAACA CACACGCCGA CTACAGCCAG 
GACGGACAGC TACGGATGCT GCTAAACCAG CCAATGCAAA CACTTGTACA AGACAGCAAT 
GGGGGGCCCC AGTCTTCCCA ATGCACCTCA ACTATTCAAC AAACCAAATA TCCTCTGGAG 
CAGCAGATGA TGGCTCCTTC CTTCCCTGTG CAGCAGGTGA ACTGTAACGC TGTGCTAGTC 
CCCTCCCCCG TCTTCACCTC ACCAATCATG ATCCCCCATA ATAGTTTCAT CACCCATCAG 
ACCACCCCCA CCTACACGAC TCACCCGCCG GTTGCCCCAC ACAGCTTACC ACTACAGCAG 
CCTCAGCAGT TCTTTCAGAT GCAGCCTCAG GGTCTGATTC ACGGCACTCC CACTCAGGCC 
TTATTCCACA CCCCTAGTGT TCCACAGCAA AGCACTGTGG GATACGTACA GCCACAACAG 
CAGCAGCAGC AACAGCAGCA TCATCATCAC TCCCAGTCTG GCAACCTGTC GGACTACAGA 
AACATGCTCC CTCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

npas2 ( XM_005165121.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165178.1 Danio rerio neuronal PAS domain protein 2 (npas2), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa10247
Clone ID Related Accession (Same CDS sequence)	XM_005165121.4 , XM_021476023.1 , XM_005165120.3 , XM_009301290.2 , XM_005165121.3 , XM_005165120.4
Accession Version	XM_005165121.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	circadian clock protein PASD1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005165121.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGAAG GAGTGAGCAT GGACAACCTT TCGGAGTTTG GAGGCCTTTG TCCGTCCAGC 
CGGAGGGAAT GGGACACAAG CAGTTGTGTG GATGATTTAA TGGACGAAGA TGAGAAGGAC 
AGAGCAAAAA GGGCGTCCCG GAACAAATCG GAGAAGAAGA GGCGAGACCA GTTCAATGTT 
CTCATTAAGG AGCTGTGTAC AATGCTGCAG GGTCAAGGTC ACCCCCGCAA GATGGACAAA 
TCCACCATCC TGCAGAGGAC CATCGACTTC CTGCAAAAAC AGAAAGAGAT CACGGCACAA 
ACAGAGTCTT GTGAGGTCCG GCAGGACTGG AAGCCTTCTT TCCTCAGCAA CGAGGAGTTC 
ACCCAGTTAA TGCTGGAGGC GTTGGATGGT TTTCTAATAG CTCTTACCAC AGATGGGAAC 
ATCATATATG TATCTGACAG CGTCTCGTCT CTCATTGGCC ATCTGCCGTC AGATATGGTG 
GACCAGAACA TCCTGAACTT CCTGCCAGAG CGAGAGCACG CAGATGTGTA TAAACTCCTG 
TCTTCACACA TGCTTCTGAC TGAGTCTTCT ACTGTAGACC TGCTCAACAG CAATGAGACA 
CACGTCGAGT TCTGCTGTCA TATAGCGAGG GGAAACATTG ACCCGAAAGA ACCGCCTACG 
TACGAATATG TCAAATTTGT TGGAGATTTC AAGTTTCATA ATAATGTGCC TCTGTCTTCA 
TGTAATGGTT ATGATCTGGC GTTTCCTCGC ACGCTGCAGT CCTCAATAGA GGAGCAGGTC 
TGCCTCGTGG CCACAGTCAG ACTCGCAACC CCTCAGTTCC TCAAGGATCT TTGCAACGTG 
GAGGATGTGT GTGATGAATT CACATCAAGA CACAGTCTGG AATGGAAATT TCTCTTCTTA 
GATCACAGGG CCTCTCCTAT CATCGGGTAC CTGCCGTTTG AGGTTTTGGG GACCTCGGGT 
TATGATTACT ACCATGTGGA CGACCTGGAG CTGATTGCTC AATGTCATAA ACAATTGATG 
CAGTGTGGGA AAGGAAAGTC TTGTTACTAT CGGTTCCTGA CTAAAGGACA GCAGTGGATC 
TGGCTGCAGA CGCACTATTA CATCACGTAC CACCAGTGGA ACTCCAAACC TGAGTTCATC 
GTCTGCACAC ACAGCGTGGT CAGTTATGCT GAAGTTCGAG CGGAGAGAAG GAGAGAGTTT 
GGATTGGAGG AGAGCTCATC TGATATGGCC ACATCCTCAA TCAAAGGCCA GGAGGTGTTT 
TTGGACATGT GTCCTCCTCT GGAAGCTACA CGGGACAGAA TCAACAGCGC TCGATCAGTG 
TCATCACAGA GCTCCAGAAA ATCATCACAC ACAGCGCTCT CCGACTCCGC ATCGAACTCC 
TCAGTGAGAT ACACAGAAGC ATGTACGTCA TCCCGGCAGT CTGCTTCCAT CGGCCCTGAG 
AAGAGCTCCA ACAGAATGAC ACACACTGGA ACAAAGACCC TGATTCAGAG ACAGAGCTCT 
TCAGAGCCCC CGTCACTCTC TCCGTCCTGC AGTCAGCATT CAGCCATGAC TCAGTCCCTG 
TACAGCTTCC AGCAGCCTCA GTTAGGTGTC ATGCATCAGC TAAAGGAGCA GCTGGAGGAG 
CGAACGCGGA TCCTACAGGC CGACATCAAA ACACAACAGC AAGAGCTGCA CGACATTAAA 
GAGAAGCTGC AGCTCGCCAA CCTACAGATG TTACTCCAGC AGCCGATACA GGGAGAGTTT 
GCTGGTTCTG GTCCACAGCA GCAGGGTCCA GGCAGGCCGG CTCAACAAAA CACACACCCT 
AAACCTCAAC ACTGTGTTTC ACATTCATCA CACACACTAC AACCCAACAG CTCATCCACT 
CAGGGGCAGC AGAGGCTGGT TCAGGCTGGA CACATGCAGG CCGTCAGTGT TCCAATGCAG 
ACACACACCA GTCTGACCAC ACCCTTCTAC AGCAACCCCA TGATGTTCTC CCCCAACCAC 
GGACACCGAA GCCAACATGA CACACACTGC CAAAGAAACA CACACGCCGA CTACAGCCAG 
GACGGACAGC TACGGATGCT GCTAAACCAG CCAATGCAAA CACTTGTACA AGACAGCAAT 
GGGGGGCCCC AGTCTTCCCA ATGCACCTCA ACTATTCAAC AAACCAAATA TCCTCTGGAG 
CAGCAGATGA TGGCTCCTTC CTTCCCTGTG CAGCAGGTGA ACTGTAACGC TGTGCTAGTC 
CCCTCCCCCG TCTTCACCTC ACCAATCATG ATCCCCCATA ATAGTTTCAT CACCCATCAG 
ACCACCCCCA CCTACACGAC TCACCCGCCG GTTGCCCCAC ACAGCTTACC ACTACAGCAG 
CCTCAGCAGT TCTTTCAGAT GCAGCCTCAG GGTCTGATTC ACGGCACTCC CACTCAGGCC 
TTATTCCACA CCCCTAGTGT TCCACAGCAA AGCACTGTGG GATACGTACA GCCACAACAG 
CAGCAGCAGC AACAGCAGCA TCATCATCAC TCCCAGTCTG GCAACCTGTC GGACTACAGA 
AACATGCTCC CTCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165178.1
CDS	813..3350
Translation	MGEGVSMDNLSEFGGLCPSSRREWDTSSCVDDLMDEDEKDRAKRASRNKSEKKRRDQFNVLIKELCTMLQGQGHPRKMDKSTILQRTIDFLQKQKEITAQTESCEVRQDWKPSFLSNEEFTQLMLEALDGFLIALTTDGNIIYVSDSVSSLIGHLPSDMVDQNILNFLPEREHADVYKLLSSHMLLTESSTVDLLNSNETHVEFCCHIARGNIDPKEPPTYEYVKFVGDFKFHNNVPLSSCNGYDLAFPRTLQSSIEEQVCLVATVRLATPQFLKDLCNVEDVCDEFTSRHSLEWKFLFLDHRASPIIGYLPFEVLGTSGYDYYHVDDLELIAQCHKQLMQCGKGKSCYYRFLTKGQQWIWLQTHYYITYHQWNSKPEFIVCTHSVVSYAEVRAERRREFGLEESSSDMATSSIKGQEVFLDMCPPLEATRDRINSARSVSSQSSRKSSHTALSDSASNSSVRYTEACTSSRQSASIGPEKSSNRMTHTGTKTLIQRQSSSEPPSLSPSCSQHSAMTQSLYSFQQPQLGVMHQLKEQLEERTRILQADIKTQQQELHDIKEKLQLANLQMLLQQPIQGEFAGSGPQQQGPGRPAQQNTHPKPQHCVSHSSHTLQPNSSSTQGQQRLVQAGHMQAVSVPMQTHTSLTTPFYSNPMMFSPNHGHRSQHDTHCQRNTHADYSQDGQLRMLLNQPMQTLVQDSNGGPQSSQCTSTIQQTKYPLEQQMMAPSFPVQQVNCNAVLVPSPVFTSPIMIPHNSFITHQTTPTYTTHPPVAPHSLPLQQPQQFFQMQPQGLIHGTPTQALFHTPSVPQQSTVGYVQPQQQQQQQQHHHHSQSGNLSDYRNMLPR

Target ORF information:

RefSeq Version	XM_005165121.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio neuronal PAS domain protein 2 (npas2), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165121.3

ORF Insert Sequence:

ATGGGAGAAG GAGTGAGCAT GGACAACCTT TCGGAGTTTG GAGGCCTTTG TCCGTCCAGC 
CGGAGGGAAT GGGACACAAG CAGTTGTGTG GATGATTTAA TGGACGAAGA TGAGAAGGAC 
AGAGCAAAAA GGGCGTCCCG GAACAAATCG GAGAAGAAGA GGCGAGACCA GTTCAATGTT 
CTCATTAAGG AGCTGTGTAC AATGCTGCAG GGTCAAGGTC ACCCCCGCAA GATGGACAAA 
TCCACCATCC TGCAGAGGAC CATCGACTTC CTGCAAAAAC AGAAAGAGAT CACGGCACAA 
ACAGAGTCTT GTGAGGTCCG GCAGGACTGG AAGCCTTCTT TCCTCAGCAA CGAGGAGTTC 
ACCCAGTTAA TGCTGGAGGC GTTGGATGGT TTTCTAATAG CTCTTACCAC AGATGGGAAC 
ATCATATATG TATCTGACAG CGTCTCGTCT CTCATTGGCC ATCTGCCGTC AGATATGGTG 
GACCAGAACA TCCTGAACTT CCTGCCAGAG CGAGAGCACG CAGATGTGTA TAAACTCCTG 
TCTTCACACA TGCTTCTGAC TGAGTCTTCT ACTGTAGACC TGCTCAACAG CAATGAGACA 
CACGTCGAGT TCTGCTGTCA TATAGCGAGG GGAAACATTG ACCCGAAAGA ACCGCCTACG 
TACGAATATG TCAAATTTGT TGGAGATTTC AAGTTTCATA ATAATGTGCC TCTGTCTTCA 
TGTAATGGTT ATGATCTGGC GTTTCCTCGC ACGCTGCAGT CCTCAATAGA GGAGCAGGTC 
TGCCTCGTGG CCACAGTCAG ACTCGCAACC CCTCAGTTCC TCAAGGATCT TTGCAACGTG 
GAGGATGTGT GTGATGAATT CACATCAAGA CACAGTCTGG AATGGAAATT TCTCTTCTTA 
GATCACAGGG CCTCTCCTAT CATCGGGTAC CTGCCGTTTG AGGTTTTGGG GACCTCGGGT 
TATGATTACT ACCATGTGGA CGACCTGGAG CTGATTGCTC AATGTCATAA ACAATTGATG 
CAGTGTGGGA AAGGAAAGTC TTGTTACTAT CGGTTCCTGA CTAAAGGACA GCAGTGGATC 
TGGCTGCAGA CGCACTATTA CATCACGTAC CACCAGTGGA ACTCCAAACC TGAGTTCATC 
GTCTGCACAC ACAGCGTGGT CAGTTATGCT GAAGTTCGAG CGGAGAGAAG GAGAGAGTTT 
GGATTGGAGG AGAGCTCATC TGATATGGCC ACATCCTCAA TCAAAGGCCA GGAGGTGTTT 
TTGGACATGT GTCCTCCTCT GGAAGCTACA CGGGACAGAA TCAACAGCGC TCGATCAGTG 
TCATCACAGA GCTCCAGAAA ATCATCACAC ACAGCGCTCT CCGACTCCGC ATCGAACTCC 
TCAGTGAGAT ACACAGAAGC ATGTACGTCA TCCCGGCAGT CTGCTTCCAT CGGCCCTGAG 
AAGAGCTCCA ACAGAATGAC ACACACTGGA ACAAAGACCC TGATTCAGAG ACAGAGCTCT 
TCAGAGCCCC CGTCACTCTC TCCGTCCTGC AGTCAGCATT CAGCCATGAC TCAGTCCCTG 
TACAGCTTCC AGCAGCCTCA GTTAGGTGTC ATGCATCAGC TAAAGGAGCA GCTGGAGGAG 
CGAACGCGGA TCCTACAGGC CGACATCAAA ACACAACAGC AAGAGCTGCA CGACATTAAA 
GAGAAGCTGC AGCTCGCCAA CCTACAGATG TTACTCCAGC AGCCGATACA GGGAGAGTTT 
GCTGGTTCTG GTCCACAGCA GCAGGGTCCA GGCAGGCCGG CTCAACAAAA CACACACCCT 
AAACCTCAAC ACTGTGTTTC ACATTCATCA CACACACTAC AACCCAACAG CTCATCCACT 
CAGGGGCAGC AGAGGCTGGT TCAGGCTGGA CACATGCAGG CCGTCAGTGT TCCAATGCAG 
ACACACACCA GTCTGACCAC ACCCTTCTAC AGCAACCCCA TGATGTTCTC CCCCAACCAC 
GGACACCGAA GCCAACATGA CACACACTGC CAAAGAAACA CACACGCCGA CTACAGCCAG 
GACGGACAGC TACGGATGCT GCTAAACCAG CCAATGCAAA CACTTGTACA AGACAGCAAT 
GGGGGGCCCC AGTCTTCCCA ATGCACCTCA ACTATTCAAC AAACCAAATA TCCTCTGGAG 
CAGCAGATGA TGGCTCCTTC CTTCCCTGTG CAGCAGGTGA ACTGTAACGC TGTGCTAGTC 
CCCTCCCCCG TCTTCACCTC ACCAATCATG ATCCCCCATA ATAGTTTCAT CACCCATCAG 
ACCACCCCCA CCTACACGAC TCACCCGCCG GTTGCCCCAC ACAGCTTACC ACTACAGCAG 
CCTCAGCAGT TCTTTCAGAT GCAGCCTCAG GGTCTGATTC ACGGCACTCC CACTCAGGCC 
TTATTCCACA CCCCTAGTGT TCCACAGCAA AGCACTGTGG GATACGTACA GCCACAACAG 
CAGCAGCAGC AACAGCAGCA TCATCATCAC TCCCAGTCTG GCAACCTGTC GGACTACAGA 
AACATGCTCC CTCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

npas2 ( XM_005165120.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005165177.1 Danio rerio neuronal PAS domain protein 2 (npas2), transcript variant X2, mRNA.

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CloneID	ODa10247
Clone ID Related Accession (Same CDS sequence)	XM_005165121.4 , XM_021476023.1 , XM_005165120.3 , XM_009301290.2 , XM_005165121.3 , XM_005165120.4
Accession Version	XM_005165120.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2538bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	circadian clock protein PASD1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007116.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005165120.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGAGAAG GAGTGAGCAT GGACAACCTT TCGGAGTTTG GAGGCCTTTG TCCGTCCAGC 
CGGAGGGAAT GGGACACAAG CAGTTGTGTG GATGATTTAA TGGACGAAGA TGAGAAGGAC 
AGAGCAAAAA GGGCGTCCCG GAACAAATCG GAGAAGAAGA GGCGAGACCA GTTCAATGTT 
CTCATTAAGG AGCTGTGTAC AATGCTGCAG GGTCAAGGTC ACCCCCGCAA GATGGACAAA 
TCCACCATCC TGCAGAGGAC CATCGACTTC CTGCAAAAAC AGAAAGAGAT CACGGCACAA 
ACAGAGTCTT GTGAGGTCCG GCAGGACTGG AAGCCTTCTT TCCTCAGCAA CGAGGAGTTC 
ACCCAGTTAA TGCTGGAGGC GTTGGATGGT TTTCTAATAG CTCTTACCAC AGATGGGAAC 
ATCATATATG TATCTGACAG CGTCTCGTCT CTCATTGGCC ATCTGCCGTC AGATATGGTG 
GACCAGAACA TCCTGAACTT CCTGCCAGAG CGAGAGCACG CAGATGTGTA TAAACTCCTG 
TCTTCACACA TGCTTCTGAC TGAGTCTTCT ACTGTAGACC TGCTCAACAG CAATGAGACA 
CACGTCGAGT TCTGCTGTCA TATAGCGAGG GGAAACATTG ACCCGAAAGA ACCGCCTACG 
TACGAATATG TCAAATTTGT TGGAGATTTC AAGTTTCATA ATAATGTGCC TCTGTCTTCA 
TGTAATGGTT ATGATCTGGC GTTTCCTCGC ACGCTGCAGT CCTCAATAGA GGAGCAGGTC 
TGCCTCGTGG CCACAGTCAG ACTCGCAACC CCTCAGTTCC TCAAGGATCT TTGCAACGTG 
GAGGATGTGT GTGATGAATT CACATCAAGA CACAGTCTGG AATGGAAATT TCTCTTCTTA 
GATCACAGGG CCTCTCCTAT CATCGGGTAC CTGCCGTTTG AGGTTTTGGG GACCTCGGGT 
TATGATTACT ACCATGTGGA CGACCTGGAG CTGATTGCTC AATGTCATAA ACAATTGATG 
CAGTGTGGGA AAGGAAAGTC TTGTTACTAT CGGTTCCTGA CTAAAGGACA GCAGTGGATC 
TGGCTGCAGA CGCACTATTA CATCACGTAC CACCAGTGGA ACTCCAAACC TGAGTTCATC 
GTCTGCACAC ACAGCGTGGT CAGTTATGCT GAAGTTCGAG CGGAGAGAAG GAGAGAGTTT 
GGATTGGAGG AGAGCTCATC TGATATGGCC ACATCCTCAA TCAAAGGCCA GGAGGTGTTT 
TTGGACATGT GTCCTCCTCT GGAAGCTACA CGGGACAGAA TCAACAGCGC TCGATCAGTG 
TCATCACAGA GCTCCAGAAA ATCATCACAC ACAGCGCTCT CCGACTCCGC ATCGAACTCC 
TCAGTGAGAT ACACAGAAGC ATGTACGTCA TCCCGGCAGT CTGCTTCCAT CGGCCCTGAG 
AAGAGCTCCA ACAGAATGAC ACACACTGGA ACAAAGACCC TGATTCAGAG ACAGAGCTCT 
TCAGAGCCCC CGTCACTCTC TCCGTCCTGC AGTCAGCATT CAGCCATGAC TCAGTCCCTG 
TACAGCTTCC AGCAGCCTCA GTTAGGTGTC ATGCATCAGC TAAAGGAGCA GCTGGAGGAG 
CGAACGCGGA TCCTACAGGC CGACATCAAA ACACAACAGC AAGAGCTGCA CGACATTAAA 
GAGAAGCTGC AGCTCGCCAA CCTACAGATG TTACTCCAGC AGCCGATACA GGGAGAGTTT 
GCTGGTTCTG GTCCACAGCA GCAGGGTCCA GGCAGGCCGG CTCAACAAAA CACACACCCT 
AAACCTCAAC ACTGTGTTTC ACATTCATCA CACACACTAC AACCCAACAG CTCATCCACT 
CAGGGGCAGC AGAGGCTGGT TCAGGCTGGA CACATGCAGG CCGTCAGTGT TCCAATGCAG 
ACACACACCA GTCTGACCAC ACCCTTCTAC AGCAACCCCA TGATGTTCTC CCCCAACCAC 
GGACACCGAA GCCAACATGA CACACACTGC CAAAGAAACA CACACGCCGA CTACAGCCAG 
GACGGACAGC TACGGATGCT GCTAAACCAG CCAATGCAAA CACTTGTACA AGACAGCAAT 
GGGGGGCCCC AGTCTTCCCA ATGCACCTCA ACTATTCAAC AAACCAAATA TCCTCTGGAG 
CAGCAGATGA TGGCTCCTTC CTTCCCTGTG CAGCAGGTGA ACTGTAACGC TGTGCTAGTC 
CCCTCCCCCG TCTTCACCTC ACCAATCATG ATCCCCCATA ATAGTTTCAT CACCCATCAG 
ACCACCCCCA CCTACACGAC TCACCCGCCG GTTGCCCCAC ACAGCTTACC ACTACAGCAG 
CCTCAGCAGT TCTTTCAGAT GCAGCCTCAG GGTCTGATTC ACGGCACTCC CACTCAGGCC 
TTATTCCACA CCCCTAGTGT TCCACAGCAA AGCACTGTGG GATACGTACA GCCACAACAG 
CAGCAGCAGC AACAGCAGCA TCATCATCAC TCCCAGTCTG GCAACCTGTC GGACTACAGA 
AACATGCTCC CTCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005165177.1
CDS	543..3080
Translation	MGEGVSMDNLSEFGGLCPSSRREWDTSSCVDDLMDEDEKDRAKRASRNKSEKKRRDQFNVLIKELCTMLQGQGHPRKMDKSTILQRTIDFLQKQKEITAQTESCEVRQDWKPSFLSNEEFTQLMLEALDGFLIALTTDGNIIYVSDSVSSLIGHLPSDMVDQNILNFLPEREHADVYKLLSSHMLLTESSTVDLLNSNETHVEFCCHIARGNIDPKEPPTYEYVKFVGDFKFHNNVPLSSCNGYDLAFPRTLQSSIEEQVCLVATVRLATPQFLKDLCNVEDVCDEFTSRHSLEWKFLFLDHRASPIIGYLPFEVLGTSGYDYYHVDDLELIAQCHKQLMQCGKGKSCYYRFLTKGQQWIWLQTHYYITYHQWNSKPEFIVCTHSVVSYAEVRAERRREFGLEESSSDMATSSIKGQEVFLDMCPPLEATRDRINSARSVSSQSSRKSSHTALSDSASNSSVRYTEACTSSRQSASIGPEKSSNRMTHTGTKTLIQRQSSSEPPSLSPSCSQHSAMTQSLYSFQQPQLGVMHQLKEQLEERTRILQADIKTQQQELHDIKEKLQLANLQMLLQQPIQGEFAGSGPQQQGPGRPAQQNTHPKPQHCVSHSSHTLQPNSSSTQGQQRLVQAGHMQAVSVPMQTHTSLTTPFYSNPMMFSPNHGHRSQHDTHCQRNTHADYSQDGQLRMLLNQPMQTLVQDSNGGPQSSQCTSTIQQTKYPLEQQMMAPSFPVQQVNCNAVLVPSPVFTSPIMIPHNSFITHQTTPTYTTHPPVAPHSLPLQQPQQFFQMQPQGLIHGTPTQALFHTPSVPQQSTVGYVQPQQQQQQQQHHHHSQSGNLSDYRNMLPR

Target ORF information:

RefSeq Version	XM_005165120.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio neuronal PAS domain protein 2 (npas2), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005165120.4

ORF Insert Sequence:

ATGGGAGAAG GAGTGAGCAT GGACAACCTT TCGGAGTTTG GAGGCCTTTG TCCGTCCAGC 
CGGAGGGAAT GGGACACAAG CAGTTGTGTG GATGATTTAA TGGACGAAGA TGAGAAGGAC 
AGAGCAAAAA GGGCGTCCCG GAACAAATCG GAGAAGAAGA GGCGAGACCA GTTCAATGTT 
CTCATTAAGG AGCTGTGTAC AATGCTGCAG GGTCAAGGTC ACCCCCGCAA GATGGACAAA 
TCCACCATCC TGCAGAGGAC CATCGACTTC CTGCAAAAAC AGAAAGAGAT CACGGCACAA 
ACAGAGTCTT GTGAGGTCCG GCAGGACTGG AAGCCTTCTT TCCTCAGCAA CGAGGAGTTC 
ACCCAGTTAA TGCTGGAGGC GTTGGATGGT TTTCTAATAG CTCTTACCAC AGATGGGAAC 
ATCATATATG TATCTGACAG CGTCTCGTCT CTCATTGGCC ATCTGCCGTC AGATATGGTG 
GACCAGAACA TCCTGAACTT CCTGCCAGAG CGAGAGCACG CAGATGTGTA TAAACTCCTG 
TCTTCACACA TGCTTCTGAC TGAGTCTTCT ACTGTAGACC TGCTCAACAG CAATGAGACA 
CACGTCGAGT TCTGCTGTCA TATAGCGAGG GGAAACATTG ACCCGAAAGA ACCGCCTACG 
TACGAATATG TCAAATTTGT TGGAGATTTC AAGTTTCATA ATAATGTGCC TCTGTCTTCA 
TGTAATGGTT ATGATCTGGC GTTTCCTCGC ACGCTGCAGT CCTCAATAGA GGAGCAGGTC 
TGCCTCGTGG CCACAGTCAG ACTCGCAACC CCTCAGTTCC TCAAGGATCT TTGCAACGTG 
GAGGATGTGT GTGATGAATT CACATCAAGA CACAGTCTGG AATGGAAATT TCTCTTCTTA 
GATCACAGGG CCTCTCCTAT CATCGGGTAC CTGCCGTTTG AGGTTTTGGG GACCTCGGGT 
TATGATTACT ACCATGTGGA CGACCTGGAG CTGATTGCTC AATGTCATAA ACAATTGATG 
CAGTGTGGGA AAGGAAAGTC TTGTTACTAT CGGTTCCTGA CTAAAGGACA GCAGTGGATC 
TGGCTGCAGA CGCACTATTA CATCACGTAC CACCAGTGGA ACTCCAAACC TGAGTTCATC 
GTCTGCACAC ACAGCGTGGT CAGTTATGCT GAAGTTCGAG CGGAGAGAAG GAGAGAGTTT 
GGATTGGAGG AGAGCTCATC TGATATGGCC ACATCCTCAA TCAAAGGCCA GGAGGTGTTT 
TTGGACATGT GTCCTCCTCT GGAAGCTACA CGGGACAGAA TCAACAGCGC TCGATCAGTG 
TCATCACAGA GCTCCAGAAA ATCATCACAC ACAGCGCTCT CCGACTCCGC ATCGAACTCC 
TCAGTGAGAT ACACAGAAGC ATGTACGTCA TCCCGGCAGT CTGCTTCCAT CGGCCCTGAG 
AAGAGCTCCA ACAGAATGAC ACACACTGGA ACAAAGACCC TGATTCAGAG ACAGAGCTCT 
TCAGAGCCCC CGTCACTCTC TCCGTCCTGC AGTCAGCATT CAGCCATGAC TCAGTCCCTG 
TACAGCTTCC AGCAGCCTCA GTTAGGTGTC ATGCATCAGC TAAAGGAGCA GCTGGAGGAG 
CGAACGCGGA TCCTACAGGC CGACATCAAA ACACAACAGC AAGAGCTGCA CGACATTAAA 
GAGAAGCTGC AGCTCGCCAA CCTACAGATG TTACTCCAGC AGCCGATACA GGGAGAGTTT 
GCTGGTTCTG GTCCACAGCA GCAGGGTCCA GGCAGGCCGG CTCAACAAAA CACACACCCT 
AAACCTCAAC ACTGTGTTTC ACATTCATCA CACACACTAC AACCCAACAG CTCATCCACT 
CAGGGGCAGC AGAGGCTGGT TCAGGCTGGA CACATGCAGG CCGTCAGTGT TCCAATGCAG 
ACACACACCA GTCTGACCAC ACCCTTCTAC AGCAACCCCA TGATGTTCTC CCCCAACCAC 
GGACACCGAA GCCAACATGA CACACACTGC CAAAGAAACA CACACGCCGA CTACAGCCAG 
GACGGACAGC TACGGATGCT GCTAAACCAG CCAATGCAAA CACTTGTACA AGACAGCAAT 
GGGGGGCCCC AGTCTTCCCA ATGCACCTCA ACTATTCAAC AAACCAAATA TCCTCTGGAG 
CAGCAGATGA TGGCTCCTTC CTTCCCTGTG CAGCAGGTGA ACTGTAACGC TGTGCTAGTC 
CCCTCCCCCG TCTTCACCTC ACCAATCATG ATCCCCCATA ATAGTTTCAT CACCCATCAG 
ACCACCCCCA CCTACACGAC TCACCCGCCG GTTGCCCCAC ACAGCTTACC ACTACAGCAG 
CCTCAGCAGT TCTTTCAGAT GCAGCCTCAG GGTCTGATTC ACGGCACTCC CACTCAGGCC 
TTATTCCACA CCCCTAGTGT TCCACAGCAA AGCACTGTGG GATACGTACA GCCACAACAG 
CAGCAGCAGC AACAGCAGCA TCATCATCAC TCCCAGTCTG GCAACCTGTC GGACTACAGA 
AACATGCTCC CTCGGTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Clock1a affects mesoderm development and primitive hematopoiesis by regulating Nodal-Smad3 signaling in the zebrafish embryo.
The Journal of biological chemistry292(34)14165-14175(2017 08)
Bian SS,Zheng XL,Sun HQ,Chen JH,Lu YL,Liu YQ,Tao DC,Ma YX

A chemical screen in zebrafish embryonic cells establishes that Akt activation is required for neural crest development.
eLife6(2017 Aug)
Ciarlo C,Kaufman CK,Kinikoglu B,Michael J,Yang S,D Amato C,Blokzijl-Franke S,den Hertog J,Schlaeger TM,Zhou Y,Liao E,Zon LI

Transcriptional alterations induced by binary mixtures of ethinylestradiol and norgestrel during the early development of zebrafish (Danio rerio).
Comparative biochemistry and physiology. Toxicology & pharmacology : CBP19560-67(2017 May)
Liang YQ,Huang GY,Zhao JL,Shi WJ,Hu LX,Tian F,Liu SS,Jiang YX,Ying GG

Genetically Blocking the Zebrafish Pineal Clock Affects Circadian Behavior.
PLoS genetics12(11)e1006445(2016 Nov)
Ben-Moshe Livne Z,Alon S,Vallone D,Bayleyen Y,Tovin A,Shainer I,Nisembaum LG,Aviram I,Smadja-Storz S,Fuentes M,Falc?n J,Eisenberg E,Klein DC,Burgess HA,Foulkes NS,Gothilf Y

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npas2 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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