selenot1b cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	selenot1b
Entrez Gene ID	352921
Full Name	selenoprotein T, 1b
Synonyms	cb698,selt1b,sept1b,wu:fb51a03,wu:fb51b01
General protein information	Preferred Names selenoprotein T, 1b Names thioredoxin reductase-like selenoprotein T1b thioredoxin reductase-like enzyme
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)
Summary	This gene encodes a selenoprotein, containing a selenocysteine (Sec) residue at the active site. Sec is encoded by the UGA codon that normally signals translation termination. The 3' UTRs of selenoprotein mRNAs in vertebrates contain a conserved stem-loop structure, the Sec insertion sequence (SECIS) element, that is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. This protein is localized in the endoplasmic reticulum. It belongs to the SelWTH family that possesses a thioredoxin-like fold and a conserved CxxU (C is cysteine, U is Sec) motif, suggesting a redox function for this gene. Paralogs of this gene are found on chromosomes 2 (GeneID:352919) and 14 (GeneID:352917). [provided by RefSeq, Aug 2017].

mRNA and Protein(s)

mRNA	Protein	Name
NM_178292.5	NP_840077.3	thioredoxin reductase-like selenoprotein T1b precursor

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	selt1b	NP_840077.3
Pan troglodytes (chimpanzee)	SELT	XP_001143719.1

Gene Ontology
Bibliography

Related articles in PubMed

Selenoprotein Gene Nomenclature.
Gladyshev VN, Arn?r ES, Berry MJ, Brigelius-Floh? R, Bruford EA, Burk RF, Carlson BA, Castellano S, Chavatte L, Conrad M, Copeland PR, Diamond AM, Driscoll DM, Ferreiro A, Floh? L, Green FR, Guig? R, Handy DE, Hatfield DL, Hesketh J, Hoffmann PR, Holmgren A, Hondal RJ, Howard MT, Huang K, Kim HY, Kim IY, K?hrle J, Krol A, Kryukov GV, Lee BJ, Lee BC, Lei XG, Liu Q, Lescure A, Lobanov AV, Loscalzo J, Maiorino M, Mariotti M, Sandeep Prabhu K, Rayman MP, Rozovsky S, Salinas G, Schmidt EE, Schomburg L, Schweizer U, Simonovi? M, Sunde RA, Tsuji PA, Tweedie S, Ursini F, Whanger PD, Zhang Y
The Journal of biological chemistry291(46)24036-24040(2016 Nov)

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

Selenium prevents downregulation of antioxidant selenoprotein genes by methylmercury.
Penglase S, Hamre K, Ellingsen S
Free radical biology & medicine7595-104(2014 Oct)

Composition and evolution of the vertebrate and mammalian selenoproteomes.
Mariotti M, Ridge PG, Zhang Y, Lobanov AV, Pringle TH, Guigo R, Hatfield DL, Gladyshev VN
PloS one7(3)e33066(2012)

Spatial and temporal expression patterns of selenoprotein genes during embryogenesis in zebrafish.
Thisse C, Degrave A, Kryukov GV, Gladyshev VN, Obrecht-Pflumio S, Krol A, Thisse B, Lescure A
Gene expression patterns : GEP3(4)525-32(2003 Aug)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following selenot1b gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the selenot1b cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa04021	NM_178292.5 Latest version!	Danio rerio selenoprotein T, 1b (selenot1b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	7-9	$104.30 $149.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

selenot1b ( NM_178292.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_840077.3 Danio rerio selenoprotein T, 1b (selenot1b), mRNA.

Price & Availability↑

CloneID	ODa04021
Clone ID Related Accession (Same CDS sequence)	NM_178292.5
Accession Version	NM_178292.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 582bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-11-14
Organism	Danio rerio(zebrafish)
Product	thioredoxin reductase-like selenoprotein T1b precursor
Comment	Comment: REVIEWED REFSEQ: This record has been curated by NCBI staff. The reference sequence was derived from CN014436.1, BX950182.13, EH502237.1, EH522934.1 and CT729866.2. On Feb 3, 2010 this sequence version replaced NM_178292.4. ##Evidence-Data-START## Transcript exon combination :: GDQH01001626.1, GFIL01034756.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END## ##RefSeq-Attributes-START## protein contains selenocysteine :: inferred from conservation ##RefSeq-Attributes-END## COMPLETENESS: complete on the 3' end.

ATGGAAACGC GGTGTTTATA TCTTTTACTG GTGTGTGTGC TGTCAGTGAA TCACGCCACA 
GCCGACAATG GCAGCATTAA AAAGATGAAA ATGCAATATA CCGGATTCCC ACTGCTGAAG 
TTTCAGATTT GTGTTTCCTG AGGGTACCGG CGGGTGTTTG AAGAGTACAC TCGGGTTCTC 
ACCCAGAGGT ATCCAGACAT CCGCATCGAG GGAGAAAATT TCCTGCCACA GCCTTTATAC 
AGGCACATTG CTTCCTTCCT CTCTGTCTTC AAACTTGTCG TGATCGGCTT AATTATCCTG 
GGCAAGAACC CTTTCACATA TTTACACATT GAGACTCCAG GAATCTGGCT TTGGGCACAA 
GAAAATAAGA TATACGCCTG CACGATGGTG TTCTTCCTCA GCAACATGAT TGAAAACCAG 
TGCATGTCCA CTGGTGCCTT CGAAGTAACC CTTAATGATG TGCCTGTATG GTCAAAGCTT 
CAGTCAGGAC ACCTGCCGTC TATGCAGCAG CTGGTGCAGA TTCTGGAGAA TGAGATGAAG 
CTGAGTGTAC ACATGGACTC TCTCCCTCAC AGACGGGCGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_840077.3
CDS	95..676
Translation	METRCLYLLLVCVLSVNHATADNGSIKKMKMQYTGFPLLKFQICVS*GYRRVFEEYTRVLTQRYPDIRIEGENFLPQPLYRHIASFLSVFKLVVIGLIILGKNPFTYLHIETPGIWLWAQENKIYACTMVFFLSNMIENQCMSTGAFEVTLNDVPVWSKLQSGHLPSMQQLVQILENEMKLSVHMDSLPHRRA

Target ORF information:

RefSeq Version	NM_178292.5
Organism	Danio rerio(zebrafish)
Definition	Danio rerio selenoprotein T, 1b (selenot1b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_178292.5

ORF Insert Sequence:

ATGGAAACGC GGTGTTTATA TCTTTTACTG GTGTGTGTGC TGTCAGTGAA TCACGCCACA 
GCCGACAATG GCAGCATTAA AAAGATGAAA ATGCAATATA CCGGATTCCC ACTGCTGAAG 
TTTCAGATTT GTGTTTCCTG AGGGTACCGG CGGGTGTTTG AAGAGTACAC TCGGGTTCTC 
ACCCAGAGGT ATCCAGACAT CCGCATCGAG GGAGAAAATT TCCTGCCACA GCCTTTATAC 
AGGCACATTG CTTCCTTCCT CTCTGTCTTC AAACTTGTCG TGATCGGCTT AATTATCCTG 
GGCAAGAACC CTTTCACATA TTTACACATT GAGACTCCAG GAATCTGGCT TTGGGCACAA 
GAAAATAAGA TATACGCCTG CACGATGGTG TTCTTCCTCA GCAACATGAT TGAAAACCAG 
TGCATGTCCA CTGGTGCCTT CGAAGTAACC CTTAATGATG TGCCTGTATG GTCAAAGCTT 
CAGTCAGGAC ACCTGCCGTC TATGCAGCAG CTGGTGCAGA TTCTGGAGAA TGAGATGAAG 
CTGAGTGTAC ACATGGACTC TCTCCCTCAC AGACGGGCGT AA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Selenoprotein Gene Nomenclature.
The Journal of biological chemistry291(46)24036-24040(2016 Nov)
Gladyshev VN,Arn?r ES,Berry MJ,Brigelius-Floh? R,Bruford EA,Burk RF,Carlson BA,Castellano S,Chavatte L,Conrad M,Copeland PR,Diamond AM,Driscoll DM,Ferreiro A,Floh? L,Green FR,Guig? R,Handy DE,Hatfield DL,Hesketh J,Hoffmann PR,Holmgren A,Hondal RJ,Howard MT,Huang K,Kim HY,Kim IY,K?hrle J,Krol A,Kryukov GV,Lee BJ,Lee BC,Lei XG,Liu Q,Lescure A,Lobanov AV,Loscalzo J,Maiorino M,Mariotti M,Sandeep Prabhu K,Rayman MP,Rozovsky S,Salinas G,Schmidt EE,Schomburg L,Schweizer U,Simonovi? M,Sunde RA,Tsuji PA,Tweedie S,Ursini F,Whanger PD,Zhang Y

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

Selenium prevents downregulation of antioxidant selenoprotein genes by methylmercury.
Free radical biology & medicine7595-104(2014 Oct)
Penglase S,Hamre K,Ellingsen S

Composition and evolution of the vertebrate and mammalian selenoproteomes.
PloS one7(3)e33066(2012)
Mariotti M,Ridge PG,Zhang Y,Lobanov AV,Pringle TH,Guigo R,Hatfield DL,Gladyshev VN

Spatial and temporal expression patterns of selenoprotein genes during embryogenesis in zebrafish.
Gene expression patterns : GEP3(4)525-32(2003 Aug)
Thisse C,Degrave A,Kryukov GV,Gladyshev VN,Obrecht-Pflumio S,Krol A,Thisse B,Lescure A

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selenot1b cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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