zgc:173742 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	zgc:173742
Entrez Gene ID	334557
Full Name	zgc:173742
Synonyms	fl27b11,si:dkey-204j6.1,wu:fl27b11
General protein information	Preferred Names zgc:173742 Names DNA topoisomerase I, mitochondrial uncharacterized protein LOC334557
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005169957.3	XP_005170014.1	DNA topoisomerase I, mitochondrial
XM_021473249.1	XP_021328924.1	DNA topoisomerase I, mitochondrial isoform X2
XM_002665326.5	XP_002665372.2	DNA topoisomerase I, mitochondrial
XM_005169957.4	XP_005170014.1	DNA topoisomerase I, mitochondrial isoform X1
XM_002665326.6	XP_002665372.2	DNA topoisomerase I, mitochondrial isoform X1
XM_021473248.1	XP_021328923.1	DNA topoisomerase I, mitochondrial isoform X2

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	zgc:173742	XP_002665372.2
Xenopus tropicalis (tropical clawed frog)	top1.2	NP_001182621.1

Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following zgc:173742 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the zgc:173742 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa23182	XM_005169957.4 Latest version!	Danio rerio zgc:173742 (zgc:173742), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa23182	XM_002665326.6 Latest version!	Danio rerio zgc:173742 (zgc:173742), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa145991	XM_021473248.1 Latest version!	Danio rerio zgc:173742 (zgc:173742), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
ODa145991	XM_021473249.1 Latest version!	Danio rerio zgc:173742 (zgc:173742), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00
View Old Accession Versions >>
ODa23182	XM_005169957.3	Danio rerio zgc:173742 (zgc:173742), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
ODa23182	XM_002665326.5	Danio rerio zgc:173742 (zgc:173742), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$426.30 $609.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

zgc:173742 ( XM_005169957.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170014.1 Danio rerio zgc:173742 (zgc:173742), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa23182
Clone ID Related Accession (Same CDS sequence)	XM_005169957.3 , XM_002665326.5 , XM_005169957.4 , XM_002665326.6
Accession Version	XM_005169957.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	DNA topoisomerase I, mitochondrial
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005169957.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAACG AGTTGAAAGA CGATCCATGC TCGAGTGAAG AGGCATTTTC AGAAAAGCCC 
AAGAAAATGA AAGCAAAGGC CAAAGGCAAG ACAAAGTCTC AGCGTGAAGG GAAGGAGAAA 
ACGCACAGGA AGATGAAAGC ATCGGAGGAG GAGATCATGG AAACTGAAGG CAGTGGTGAC 
AGAAGGTCTC ATGTAAATGT TAAGGAAGAG CCAAAGGAAG ATCCAGACTT TGTAACAGAA 
AAGAAAGGGA AAAAGAGGAA AAGAAAGGCG GATAATACAG ATGGACTCTT ATCTAGCGCC 
GAAGCACCTT TGCCAAAGAA GAAGAAGAAG AAAGTTGCAG ATGGACAGGA AAACGCTTCA 
ACAGCAAAGC AGAAAAAAAT GAAGGAAAAA AAAGAAAAGG TGAAAAAAGA GGAAGGAGAG 
CGTCCGGTCT CTACTAAGAA GAAAAAAACC AAGGAGGAGA TTGAAGCAGC CAGAGCTCTC 
AAGATAAAGA AGAAAGAGGA GGAAGAGAAA CAAAGATGGA GATGGTGGGA GGAAGAGAAG 
TATGAGAATG GAATTAAATG GAAGTTTCTA GAGCATAAAG GACCATATTT CCCTCCTGCG 
TATCAGCCCC TTCCTGATGA TGTCAAGTTT TACTATAATG GAGAGCCAGT GAAACTCAGC 
CTGCCAGCTG AGGAAGTGGC CCTTTACTTT GCTCAGATGC TGGATCACGA ATACACCACC 
AAAGAGGTCT TCCGCAATAA CTTCTTCAAG GACTGGAGGA AGGAAATGAC TCTAGAGGAG 
AGACAACTAA TCATGGATCT GAAAAAGTGT GATTTTGGGG AGCTCCATGC CATGCATAAG 
GAGAAGGTGG AAGCCAGGAA AAACCTGAGC AAAGAGGAGA AGTTGGTTAA TAAAGAGGCC 
AATCAAAAAA TAGTCGACGA ATATGGGTTC TGTGTGTTGG ACCACCATCG TGAACGCATT 
GGCAACTTTA AGATTGAACC TCCAGGTCTT TTCCGAGGCA GAGGAGACCA TCCTAAACAG 
GGAATGCTGA AGAAGAGGAT TCGACCTGAG GATGTTATCA TCAACTGCAG CAAAGAATCT 
CGTATCCCAG AAGCCCCAGC CGGTCACAAA TGGAAGGAAG TTCGTCATGA CAAAACCGTA 
ACATGGCTAG CAAGCTGGAC GGAGAATGTT CAAGGATCCT GTAAATATGT AATGCTCAAT 
GCCAACTCCA AACTTAAGGG TGAGAAGGAT TGGGAAAAGT ATGAAGTGGC CCGTAAACTG 
AAGACGTGTG TGGATGCCAT CCGAACCCAG TACCAGGAGG ACCTCAAGTC CAAACAAATG 
GGCACACGCC AGAGAGCCGT AGCCCTTTAC TTCATTGACA AGCTGGCTCT GAGAGCAGGT 
AATGAAAAGG AAGAGGGCGA GACGGCAGAC ACCGTGGGTT GTTGTTCACT CCGCGTGGAG 
CACATCACTT TACACGATGC ACTGGACGAC CAGAAGTGTG TGGTAGAGTT CGACTTCCTG 
GGTAAAGATT GCATCCGCTA CTACAACAAG GTTCCTGTGA CCAAAAGGGT TTTTAAAAAC 
CTCAAGCTTT TCATGGAAAA CAAGCAAGGA GGAGACGACC TCTTTGATCG ACTCAATACT 
GCTTCGCTGA ATAAACACCT GAGTTCCCTA ATGCCGGGAT TGACTGCAAA GGTTTTTAGG 
ACATACAATG CTTCCATAAC CCTGCAGCAA CAGCTGAAAG AGCTCAGCAA CATGAAGGAT 
AATGTGCCGG AGAAGTTGCT CTCCTATAAC AGGGCAAACC GTGCGGTTGC AGTTCTGTGT 
AACCATCAGC GGGCGCCGCC AAAGTCCTTC GATCAGTCCA TGGCCAACCT GCAGGCAAAG 
ATTGACAGCA GGAAGGAGCA GCTGGACTTG GCTAAGAAAG AGCTCAAGCA GGCCAAGAAA 
GAAGCCAAAG GGAGCTCAGA CAACAAGCTG CTGGTTGTGG TGGAGAAGAA GAAGAAAGCA 
GTCCAGAGGT GTGAGGAGCA GCTGCTGAAG ATGGAGGTTC AGGCTACAGA CAGAGAGGAG 
AACAAACAGA TCGCACTGAG CACCTCCAAA CTCAACTACC TGGATCCACG CATCAGTGTG 
GCCTGGTGTA AGAACATGGA GGTGCCGCTG GACAAAATTT ACAACAAAAC CTTGAGGGAT 
AAGTTTGCCT GGGCAGTCGA CATGACTGAA CTTGACTTTG TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170014.1
CDS	221..2428
Translation	MENELKDDPCSSEEAFSEKPKKMKAKAKGKTKSQREGKEKTHRKMKASEEEIMETEGSGDRRSHVNVKEEPKEDPDFVTEKKGKKRKRKADNTDGLLSSAEAPLPKKKKKKVADGQENASTAKQKKMKEKKEKVKKEEGERPVSTKKKKTKEEIEAARALKIKKKEEEEKQRWRWWEEEKYENGIKWKFLEHKGPYFPPAYQPLPDDVKFYYNGEPVKLSLPAEEVALYFAQMLDHEYTTKEVFRNNFFKDWRKEMTLEERQLIMDLKKCDFGELHAMHKEKVEARKNLSKEEKLVNKEANQKIVDEYGFCVLDHHRERIGNFKIEPPGLFRGRGDHPKQGMLKKRIRPEDVIINCSKESRIPEAPAGHKWKEVRHDKTVTWLASWTENVQGSCKYVMLNANSKLKGEKDWEKYEVARKLKTCVDAIRTQYQEDLKSKQMGTRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHITLHDALDDQKCVVEFDFLGKDCIRYYNKVPVTKRVFKNLKLFMENKQGGDDLFDRLNTASLNKHLSSLMPGLTAKVFRTYNASITLQQQLKELSNMKDNVPEKLLSYNRANRAVAVLCNHQRAPPKSFDQSMANLQAKIDSRKEQLDLAKKELKQAKKEAKGSSDNKLLVVVEKKKKAVQRCEEQLLKMEVQATDREENKQIALSTSKLNYLDPRISVAWCKNMEVPLDKIYNKTLRDKFAWAVDMTELDFVF

Target ORF information:

RefSeq Version	XM_005169957.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio zgc:173742 (zgc:173742), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169957.3

ORF Insert Sequence:

ATGGAAAACG AGTTGAAAGA CGATCCATGC TCGAGTGAAG AGGCATTTTC AGAAAAGCCC 
AAGAAAATGA AAGCAAAGGC CAAAGGCAAG ACAAAGTCTC AGCGTGAAGG GAAGGAGAAA 
ACGCACAGGA AGATGAAAGC ATCGGAGGAG GAGATCATGG AAACTGAAGG CAGTGGTGAC 
AGAAGGTCTC ATGTAAATGT TAAGGAAGAG CCAAAGGAAG ATCCAGACTT TGTAACAGAA 
AAGAAAGGGA AAAAGAGGAA AAGAAAGGCG GATAATACAG ATGGACTCTT ATCTAGCGCC 
GAAGCACCTT TGCCAAAGAA GAAGAAGAAG AAAGTTGCAG ATGGACAGGA AAACGCTTCA 
ACAGCAAAGC AGAAAAAAAT GAAGGAAAAA AAAGAAAAGG TGAAAAAAGA GGAAGGAGAG 
CGTCCGGTCT CTACTAAGAA GAAAAAAACC AAGGAGGAGA TTGAAGCAGC CAGAGCTCTC 
AAGATAAAGA AGAAAGAGGA GGAAGAGAAA CAAAGATGGA GATGGTGGGA GGAAGAGAAG 
TATGAGAATG GAATTAAATG GAAGTTTCTA GAGCATAAAG GACCATATTT CCCTCCTGCG 
TATCAGCCCC TTCCTGATGA TGTCAAGTTT TACTATAATG GAGAGCCAGT GAAACTCAGC 
CTGCCAGCTG AGGAAGTGGC CCTTTACTTT GCTCAGATGC TGGATCACGA ATACACCACC 
AAAGAGGTCT TCCGCAATAA CTTCTTCAAG GACTGGAGGA AGGAAATGAC TCTAGAGGAG 
AGACAACTAA TCATGGATCT GAAAAAGTGT GATTTTGGGG AGCTCCATGC CATGCATAAG 
GAGAAGGTGG AAGCCAGGAA AAACCTGAGC AAAGAGGAGA AGTTGGTTAA TAAAGAGGCC 
AATCAAAAAA TAGTCGACGA ATATGGGTTC TGTGTGTTGG ACCACCATCG TGAACGCATT 
GGCAACTTTA AGATTGAACC TCCAGGTCTT TTCCGAGGCA GAGGAGACCA TCCTAAACAG 
GGAATGCTGA AGAAGAGGAT TCGACCTGAG GATGTTATCA TCAACTGCAG CAAAGAATCT 
CGTATCCCAG AAGCCCCAGC CGGTCACAAA TGGAAGGAAG TTCGTCATGA CAAAACCGTA 
ACATGGCTAG CAAGCTGGAC GGAGAATGTT CAAGGATCCT GTAAATATGT AATGCTCAAT 
GCCAACTCCA AACTTAAGGG TGAGAAGGAT TGGGAAAAGT ATGAAGTGGC CCGTAAACTG 
AAGACGTGTG TGGATGCCAT CCGAACCCAG TACCAGGAGG ACCTCAAGTC CAAACAAATG 
GGCACACGCC AGAGAGCCGT AGCCCTTTAC TTCATTGACA AGCTGGCTCT GAGAGCAGGT 
AATGAAAAGG AAGAGGGCGA GACGGCAGAC ACCGTGGGTT GTTGTTCACT CCGCGTGGAG 
CACATCACTT TACACGATGC ACTGGACGAC CAGAAGTGTG TGGTAGAGTT CGACTTCCTG 
GGTAAAGATT GCATCCGCTA CTACAACAAG GTTCCTGTGA CCAAAAGGGT TTTTAAAAAC 
CTCAAGCTTT TCATGGAAAA CAAGCAAGGA GGAGACGACC TCTTTGATCG ACTCAATACT 
GCTTCGCTGA ATAAACACCT GAGTTCCCTA ATGCCGGGAT TGACTGCAAA GGTTTTTAGG 
ACATACAATG CTTCCATAAC CCTGCAGCAA CAGCTGAAAG AGCTCAGCAA CATGAAGGAT 
AATGTGCCGG AGAAGTTGCT CTCCTATAAC AGGGCAAACC GTGCGGTTGC AGTTCTGTGT 
AACCATCAGC GGGCGCCGCC AAAGTCCTTC GATCAGTCCA TGGCCAACCT GCAGGCAAAG 
ATTGACAGCA GGAAGGAGCA GCTGGACTTG GCTAAGAAAG AGCTCAAGCA GGCCAAGAAA 
GAAGCCAAAG GGAGCTCAGA CAACAAGCTG CTGGTTGTGG TGGAGAAGAA GAAGAAAGCA 
GTCCAGAGGT GTGAGGAGCA GCTGCTGAAG ATGGAGGTTC AGGCTACAGA CAGAGAGGAG 
AACAAACAGA TCGCACTGAG CACCTCCAAA CTCAACTACC TGGATCCACG CATCAGTGTG 
GCCTGGTGTA AGAACATGGA GGTGCCGCTG GACAAAATTT ACAACAAAAC CTTGAGGGAT 
AAGTTTGCCT GGGCAGTCGA CATGACTGAA CTTGACTTTG TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zgc:173742 ( XM_002665326.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_002665372.2 Danio rerio zgc:173742 (zgc:173742), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa23182
Clone ID Related Accession (Same CDS sequence)	XM_005169957.3 , XM_002665326.5 , XM_005169957.4 , XM_002665326.6
Accession Version	XM_002665326.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	DNA topoisomerase I, mitochondrial
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_002665326.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAACG AGTTGAAAGA CGATCCATGC TCGAGTGAAG AGGCATTTTC AGAAAAGCCC 
AAGAAAATGA AAGCAAAGGC CAAAGGCAAG ACAAAGTCTC AGCGTGAAGG GAAGGAGAAA 
ACGCACAGGA AGATGAAAGC ATCGGAGGAG GAGATCATGG AAACTGAAGG CAGTGGTGAC 
AGAAGGTCTC ATGTAAATGT TAAGGAAGAG CCAAAGGAAG ATCCAGACTT TGTAACAGAA 
AAGAAAGGGA AAAAGAGGAA AAGAAAGGCG GATAATACAG ATGGACTCTT ATCTAGCGCC 
GAAGCACCTT TGCCAAAGAA GAAGAAGAAG AAAGTTGCAG ATGGACAGGA AAACGCTTCA 
ACAGCAAAGC AGAAAAAAAT GAAGGAAAAA AAAGAAAAGG TGAAAAAAGA GGAAGGAGAG 
CGTCCGGTCT CTACTAAGAA GAAAAAAACC AAGGAGGAGA TTGAAGCAGC CAGAGCTCTC 
AAGATAAAGA AGAAAGAGGA GGAAGAGAAA CAAAGATGGA GATGGTGGGA GGAAGAGAAG 
TATGAGAATG GAATTAAATG GAAGTTTCTA GAGCATAAAG GACCATATTT CCCTCCTGCG 
TATCAGCCCC TTCCTGATGA TGTCAAGTTT TACTATAATG GAGAGCCAGT GAAACTCAGC 
CTGCCAGCTG AGGAAGTGGC CCTTTACTTT GCTCAGATGC TGGATCACGA ATACACCACC 
AAAGAGGTCT TCCGCAATAA CTTCTTCAAG GACTGGAGGA AGGAAATGAC TCTAGAGGAG 
AGACAACTAA TCATGGATCT GAAAAAGTGT GATTTTGGGG AGCTCCATGC CATGCATAAG 
GAGAAGGTGG AAGCCAGGAA AAACCTGAGC AAAGAGGAGA AGTTGGTTAA TAAAGAGGCC 
AATCAAAAAA TAGTCGACGA ATATGGGTTC TGTGTGTTGG ACCACCATCG TGAACGCATT 
GGCAACTTTA AGATTGAACC TCCAGGTCTT TTCCGAGGCA GAGGAGACCA TCCTAAACAG 
GGAATGCTGA AGAAGAGGAT TCGACCTGAG GATGTTATCA TCAACTGCAG CAAAGAATCT 
CGTATCCCAG AAGCCCCAGC CGGTCACAAA TGGAAGGAAG TTCGTCATGA CAAAACCGTA 
ACATGGCTAG CAAGCTGGAC GGAGAATGTT CAAGGATCCT GTAAATATGT AATGCTCAAT 
GCCAACTCCA AACTTAAGGG TGAGAAGGAT TGGGAAAAGT ATGAAGTGGC CCGTAAACTG 
AAGACGTGTG TGGATGCCAT CCGAACCCAG TACCAGGAGG ACCTCAAGTC CAAACAAATG 
GGCACACGCC AGAGAGCCGT AGCCCTTTAC TTCATTGACA AGCTGGCTCT GAGAGCAGGT 
AATGAAAAGG AAGAGGGCGA GACGGCAGAC ACCGTGGGTT GTTGTTCACT CCGCGTGGAG 
CACATCACTT TACACGATGC ACTGGACGAC CAGAAGTGTG TGGTAGAGTT CGACTTCCTG 
GGTAAAGATT GCATCCGCTA CTACAACAAG GTTCCTGTGA CCAAAAGGGT TTTTAAAAAC 
CTCAAGCTTT TCATGGAAAA CAAGCAAGGA GGAGACGACC TCTTTGATCG ACTCAATACT 
GCTTCGCTGA ATAAACACCT GAGTTCCCTA ATGCCGGGAT TGACTGCAAA GGTTTTTAGG 
ACATACAATG CTTCCATAAC CCTGCAGCAA CAGCTGAAAG AGCTCAGCAA CATGAAGGAT 
AATGTGCCGG AGAAGTTGCT CTCCTATAAC AGGGCAAACC GTGCGGTTGC AGTTCTGTGT 
AACCATCAGC GGGCGCCGCC AAAGTCCTTC GATCAGTCCA TGGCCAACCT GCAGGCAAAG 
ATTGACAGCA GGAAGGAGCA GCTGGACTTG GCTAAGAAAG AGCTCAAGCA GGCCAAGAAA 
GAAGCCAAAG GGAGCTCAGA CAACAAGCTG CTGGTTGTGG TGGAGAAGAA GAAGAAAGCA 
GTCCAGAGGT GTGAGGAGCA GCTGCTGAAG ATGGAGGTTC AGGCTACAGA CAGAGAGGAG 
AACAAACAGA TCGCACTGAG CACCTCCAAA CTCAACTACC TGGATCCACG CATCAGTGTG 
GCCTGGTGTA AGAACATGGA GGTGCCGCTG GACAAAATTT ACAACAAAAC CTTGAGGGAT 
AAGTTTGCCT GGGCAGTCGA CATGACTGAA CTTGACTTTG TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002665372.2
CDS	283..2490
Translation	MENELKDDPCSSEEAFSEKPKKMKAKAKGKTKSQREGKEKTHRKMKASEEEIMETEGSGDRRSHVNVKEEPKEDPDFVTEKKGKKRKRKADNTDGLLSSAEAPLPKKKKKKVADGQENASTAKQKKMKEKKEKVKKEEGERPVSTKKKKTKEEIEAARALKIKKKEEEEKQRWRWWEEEKYENGIKWKFLEHKGPYFPPAYQPLPDDVKFYYNGEPVKLSLPAEEVALYFAQMLDHEYTTKEVFRNNFFKDWRKEMTLEERQLIMDLKKCDFGELHAMHKEKVEARKNLSKEEKLVNKEANQKIVDEYGFCVLDHHRERIGNFKIEPPGLFRGRGDHPKQGMLKKRIRPEDVIINCSKESRIPEAPAGHKWKEVRHDKTVTWLASWTENVQGSCKYVMLNANSKLKGEKDWEKYEVARKLKTCVDAIRTQYQEDLKSKQMGTRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHITLHDALDDQKCVVEFDFLGKDCIRYYNKVPVTKRVFKNLKLFMENKQGGDDLFDRLNTASLNKHLSSLMPGLTAKVFRTYNASITLQQQLKELSNMKDNVPEKLLSYNRANRAVAVLCNHQRAPPKSFDQSMANLQAKIDSRKEQLDLAKKELKQAKKEAKGSSDNKLLVVVEKKKKAVQRCEEQLLKMEVQATDREENKQIALSTSKLNYLDPRISVAWCKNMEVPLDKIYNKTLRDKFAWAVDMTELDFVF

Target ORF information:

RefSeq Version	XM_002665326.5
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio zgc:173742 (zgc:173742), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002665326.5

ORF Insert Sequence:

ATGGAAAACG AGTTGAAAGA CGATCCATGC TCGAGTGAAG AGGCATTTTC AGAAAAGCCC 
AAGAAAATGA AAGCAAAGGC CAAAGGCAAG ACAAAGTCTC AGCGTGAAGG GAAGGAGAAA 
ACGCACAGGA AGATGAAAGC ATCGGAGGAG GAGATCATGG AAACTGAAGG CAGTGGTGAC 
AGAAGGTCTC ATGTAAATGT TAAGGAAGAG CCAAAGGAAG ATCCAGACTT TGTAACAGAA 
AAGAAAGGGA AAAAGAGGAA AAGAAAGGCG GATAATACAG ATGGACTCTT ATCTAGCGCC 
GAAGCACCTT TGCCAAAGAA GAAGAAGAAG AAAGTTGCAG ATGGACAGGA AAACGCTTCA 
ACAGCAAAGC AGAAAAAAAT GAAGGAAAAA AAAGAAAAGG TGAAAAAAGA GGAAGGAGAG 
CGTCCGGTCT CTACTAAGAA GAAAAAAACC AAGGAGGAGA TTGAAGCAGC CAGAGCTCTC 
AAGATAAAGA AGAAAGAGGA GGAAGAGAAA CAAAGATGGA GATGGTGGGA GGAAGAGAAG 
TATGAGAATG GAATTAAATG GAAGTTTCTA GAGCATAAAG GACCATATTT CCCTCCTGCG 
TATCAGCCCC TTCCTGATGA TGTCAAGTTT TACTATAATG GAGAGCCAGT GAAACTCAGC 
CTGCCAGCTG AGGAAGTGGC CCTTTACTTT GCTCAGATGC TGGATCACGA ATACACCACC 
AAAGAGGTCT TCCGCAATAA CTTCTTCAAG GACTGGAGGA AGGAAATGAC TCTAGAGGAG 
AGACAACTAA TCATGGATCT GAAAAAGTGT GATTTTGGGG AGCTCCATGC CATGCATAAG 
GAGAAGGTGG AAGCCAGGAA AAACCTGAGC AAAGAGGAGA AGTTGGTTAA TAAAGAGGCC 
AATCAAAAAA TAGTCGACGA ATATGGGTTC TGTGTGTTGG ACCACCATCG TGAACGCATT 
GGCAACTTTA AGATTGAACC TCCAGGTCTT TTCCGAGGCA GAGGAGACCA TCCTAAACAG 
GGAATGCTGA AGAAGAGGAT TCGACCTGAG GATGTTATCA TCAACTGCAG CAAAGAATCT 
CGTATCCCAG AAGCCCCAGC CGGTCACAAA TGGAAGGAAG TTCGTCATGA CAAAACCGTA 
ACATGGCTAG CAAGCTGGAC GGAGAATGTT CAAGGATCCT GTAAATATGT AATGCTCAAT 
GCCAACTCCA AACTTAAGGG TGAGAAGGAT TGGGAAAAGT ATGAAGTGGC CCGTAAACTG 
AAGACGTGTG TGGATGCCAT CCGAACCCAG TACCAGGAGG ACCTCAAGTC CAAACAAATG 
GGCACACGCC AGAGAGCCGT AGCCCTTTAC TTCATTGACA AGCTGGCTCT GAGAGCAGGT 
AATGAAAAGG AAGAGGGCGA GACGGCAGAC ACCGTGGGTT GTTGTTCACT CCGCGTGGAG 
CACATCACTT TACACGATGC ACTGGACGAC CAGAAGTGTG TGGTAGAGTT CGACTTCCTG 
GGTAAAGATT GCATCCGCTA CTACAACAAG GTTCCTGTGA CCAAAAGGGT TTTTAAAAAC 
CTCAAGCTTT TCATGGAAAA CAAGCAAGGA GGAGACGACC TCTTTGATCG ACTCAATACT 
GCTTCGCTGA ATAAACACCT GAGTTCCCTA ATGCCGGGAT TGACTGCAAA GGTTTTTAGG 
ACATACAATG CTTCCATAAC CCTGCAGCAA CAGCTGAAAG AGCTCAGCAA CATGAAGGAT 
AATGTGCCGG AGAAGTTGCT CTCCTATAAC AGGGCAAACC GTGCGGTTGC AGTTCTGTGT 
AACCATCAGC GGGCGCCGCC AAAGTCCTTC GATCAGTCCA TGGCCAACCT GCAGGCAAAG 
ATTGACAGCA GGAAGGAGCA GCTGGACTTG GCTAAGAAAG AGCTCAAGCA GGCCAAGAAA 
GAAGCCAAAG GGAGCTCAGA CAACAAGCTG CTGGTTGTGG TGGAGAAGAA GAAGAAAGCA 
GTCCAGAGGT GTGAGGAGCA GCTGCTGAAG ATGGAGGTTC AGGCTACAGA CAGAGAGGAG 
AACAAACAGA TCGCACTGAG CACCTCCAAA CTCAACTACC TGGATCCACG CATCAGTGTG 
GCCTGGTGTA AGAACATGGA GGTGCCGCTG GACAAAATTT ACAACAAAAC CTTGAGGGAT 
AAGTTTGCCT GGGCAGTCGA CATGACTGAA CTTGACTTTG TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zgc:173742 ( XM_005169957.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170014.1 Danio rerio zgc:173742 (zgc:173742), transcript variant X2, mRNA.

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CloneID	ODa23182
Clone ID Related Accession (Same CDS sequence)	XM_005169957.3 , XM_002665326.5 , XM_005169957.4 , XM_002665326.6
Accession Version	XM_005169957.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	DNA topoisomerase I, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005169957.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAACG AGTTGAAAGA CGATCCATGC TCGAGTGAAG AGGCATTTTC AGAAAAGCCC 
AAGAAAATGA AAGCAAAGGC CAAAGGCAAG ACAAAGTCTC AGCGTGAAGG GAAGGAGAAA 
ACGCACAGGA AGATGAAAGC ATCGGAGGAG GAGATCATGG AAACTGAAGG CAGTGGTGAC 
AGAAGGTCTC ATGTAAATGT TAAGGAAGAG CCAAAGGAAG ATCCAGACTT TGTAACAGAA 
AAGAAAGGGA AAAAGAGGAA AAGAAAGGCG GATAATACAG ATGGACTCTT ATCTAGCGCC 
GAAGCACCTT TGCCAAAGAA GAAGAAGAAG AAAGTTGCAG ATGGACAGGA AAACGCTTCA 
ACAGCAAAGC AGAAAAAAAT GAAGGAAAAA AAAGAAAAGG TGAAAAAAGA GGAAGGAGAG 
CGTCCGGTCT CTACTAAGAA GAAAAAAACC AAGGAGGAGA TTGAAGCAGC CAGAGCTCTC 
AAGATAAAGA AGAAAGAGGA GGAAGAGAAA CAAAGATGGA GATGGTGGGA GGAAGAGAAG 
TATGAGAATG GAATTAAATG GAAGTTTCTA GAGCATAAAG GACCATATTT CCCTCCTGCG 
TATCAGCCCC TTCCTGATGA TGTCAAGTTT TACTATAATG GAGAGCCAGT GAAACTCAGC 
CTGCCAGCTG AGGAAGTGGC CCTTTACTTT GCTCAGATGC TGGATCACGA ATACACCACC 
AAAGAGGTCT TCCGCAATAA CTTCTTCAAG GACTGGAGGA AGGAAATGAC TCTAGAGGAG 
AGACAACTAA TCATGGATCT GAAAAAGTGT GATTTTGGGG AGCTCCATGC CATGCATAAG 
GAGAAGGTGG AAGCCAGGAA AAACCTGAGC AAAGAGGAGA AGTTGGTTAA TAAAGAGGCC 
AATCAAAAAA TAGTCGACGA ATATGGGTTC TGTGTGTTGG ACCACCATCG TGAACGCATT 
GGCAACTTTA AGATTGAACC TCCAGGTCTT TTCCGAGGCA GAGGAGACCA TCCTAAACAG 
GGAATGCTGA AGAAGAGGAT TCGACCTGAG GATGTTATCA TCAACTGCAG CAAAGAATCT 
CGTATCCCAG AAGCCCCAGC CGGTCACAAA TGGAAGGAAG TTCGTCATGA CAAAACCGTA 
ACATGGCTAG CAAGCTGGAC GGAGAATGTT CAAGGATCCT GTAAATATGT AATGCTCAAT 
GCCAACTCCA AACTTAAGGG TGAGAAGGAT TGGGAAAAGT ATGAAGTGGC CCGTAAACTG 
AAGACGTGTG TGGATGCCAT CCGAACCCAG TACCAGGAGG ACCTCAAGTC CAAACAAATG 
GGCACACGCC AGAGAGCCGT AGCCCTTTAC TTCATTGACA AGCTGGCTCT GAGAGCAGGT 
AATGAAAAGG AAGAGGGCGA GACGGCAGAC ACCGTGGGTT GTTGTTCACT CCGCGTGGAG 
CACATCACTT TACACGATGC ACTGGACGAC CAGAAGTGTG TGGTAGAGTT CGACTTCCTG 
GGTAAAGATT GCATCCGCTA CTACAACAAG GTTCCTGTGA CCAAAAGGGT TTTTAAAAAC 
CTCAAGCTTT TCATGGAAAA CAAGCAAGGA GGAGACGACC TCTTTGATCG ACTCAATACT 
GCTTCGCTGA ATAAACACCT GAGTTCCCTA ATGCCGGGAT TGACTGCAAA GGTTTTTAGG 
ACATACAATG CTTCCATAAC CCTGCAGCAA CAGCTGAAAG AGCTCAGCAA CATGAAGGAT 
AATGTGCCGG AGAAGTTGCT CTCCTATAAC AGGGCAAACC GTGCGGTTGC AGTTCTGTGT 
AACCATCAGC GGGCGCCGCC AAAGTCCTTC GATCAGTCCA TGGCCAACCT GCAGGCAAAG 
ATTGACAGCA GGAAGGAGCA GCTGGACTTG GCTAAGAAAG AGCTCAAGCA GGCCAAGAAA 
GAAGCCAAAG GGAGCTCAGA CAACAAGCTG CTGGTTGTGG TGGAGAAGAA GAAGAAAGCA 
GTCCAGAGGT GTGAGGAGCA GCTGCTGAAG ATGGAGGTTC AGGCTACAGA CAGAGAGGAG 
AACAAACAGA TCGCACTGAG CACCTCCAAA CTCAACTACC TGGATCCACG CATCAGTGTG 
GCCTGGTGTA AGAACATGGA GGTGCCGCTG GACAAAATTT ACAACAAAAC CTTGAGGGAT 
AAGTTTGCCT GGGCAGTCGA CATGACTGAA CTTGACTTTG TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170014.1
CDS	421..2628
Translation	MENELKDDPCSSEEAFSEKPKKMKAKAKGKTKSQREGKEKTHRKMKASEEEIMETEGSGDRRSHVNVKEEPKEDPDFVTEKKGKKRKRKADNTDGLLSSAEAPLPKKKKKKVADGQENASTAKQKKMKEKKEKVKKEEGERPVSTKKKKTKEEIEAARALKIKKKEEEEKQRWRWWEEEKYENGIKWKFLEHKGPYFPPAYQPLPDDVKFYYNGEPVKLSLPAEEVALYFAQMLDHEYTTKEVFRNNFFKDWRKEMTLEERQLIMDLKKCDFGELHAMHKEKVEARKNLSKEEKLVNKEANQKIVDEYGFCVLDHHRERIGNFKIEPPGLFRGRGDHPKQGMLKKRIRPEDVIINCSKESRIPEAPAGHKWKEVRHDKTVTWLASWTENVQGSCKYVMLNANSKLKGEKDWEKYEVARKLKTCVDAIRTQYQEDLKSKQMGTRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHITLHDALDDQKCVVEFDFLGKDCIRYYNKVPVTKRVFKNLKLFMENKQGGDDLFDRLNTASLNKHLSSLMPGLTAKVFRTYNASITLQQQLKELSNMKDNVPEKLLSYNRANRAVAVLCNHQRAPPKSFDQSMANLQAKIDSRKEQLDLAKKELKQAKKEAKGSSDNKLLVVVEKKKKAVQRCEEQLLKMEVQATDREENKQIALSTSKLNYLDPRISVAWCKNMEVPLDKIYNKTLRDKFAWAVDMTELDFVF

Target ORF information:

RefSeq Version	XM_005169957.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zgc:173742 (zgc:173742), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005169957.4

ORF Insert Sequence:

ATGGAAAACG AGTTGAAAGA CGATCCATGC TCGAGTGAAG AGGCATTTTC AGAAAAGCCC 
AAGAAAATGA AAGCAAAGGC CAAAGGCAAG ACAAAGTCTC AGCGTGAAGG GAAGGAGAAA 
ACGCACAGGA AGATGAAAGC ATCGGAGGAG GAGATCATGG AAACTGAAGG CAGTGGTGAC 
AGAAGGTCTC ATGTAAATGT TAAGGAAGAG CCAAAGGAAG ATCCAGACTT TGTAACAGAA 
AAGAAAGGGA AAAAGAGGAA AAGAAAGGCG GATAATACAG ATGGACTCTT ATCTAGCGCC 
GAAGCACCTT TGCCAAAGAA GAAGAAGAAG AAAGTTGCAG ATGGACAGGA AAACGCTTCA 
ACAGCAAAGC AGAAAAAAAT GAAGGAAAAA AAAGAAAAGG TGAAAAAAGA GGAAGGAGAG 
CGTCCGGTCT CTACTAAGAA GAAAAAAACC AAGGAGGAGA TTGAAGCAGC CAGAGCTCTC 
AAGATAAAGA AGAAAGAGGA GGAAGAGAAA CAAAGATGGA GATGGTGGGA GGAAGAGAAG 
TATGAGAATG GAATTAAATG GAAGTTTCTA GAGCATAAAG GACCATATTT CCCTCCTGCG 
TATCAGCCCC TTCCTGATGA TGTCAAGTTT TACTATAATG GAGAGCCAGT GAAACTCAGC 
CTGCCAGCTG AGGAAGTGGC CCTTTACTTT GCTCAGATGC TGGATCACGA ATACACCACC 
AAAGAGGTCT TCCGCAATAA CTTCTTCAAG GACTGGAGGA AGGAAATGAC TCTAGAGGAG 
AGACAACTAA TCATGGATCT GAAAAAGTGT GATTTTGGGG AGCTCCATGC CATGCATAAG 
GAGAAGGTGG AAGCCAGGAA AAACCTGAGC AAAGAGGAGA AGTTGGTTAA TAAAGAGGCC 
AATCAAAAAA TAGTCGACGA ATATGGGTTC TGTGTGTTGG ACCACCATCG TGAACGCATT 
GGCAACTTTA AGATTGAACC TCCAGGTCTT TTCCGAGGCA GAGGAGACCA TCCTAAACAG 
GGAATGCTGA AGAAGAGGAT TCGACCTGAG GATGTTATCA TCAACTGCAG CAAAGAATCT 
CGTATCCCAG AAGCCCCAGC CGGTCACAAA TGGAAGGAAG TTCGTCATGA CAAAACCGTA 
ACATGGCTAG CAAGCTGGAC GGAGAATGTT CAAGGATCCT GTAAATATGT AATGCTCAAT 
GCCAACTCCA AACTTAAGGG TGAGAAGGAT TGGGAAAAGT ATGAAGTGGC CCGTAAACTG 
AAGACGTGTG TGGATGCCAT CCGAACCCAG TACCAGGAGG ACCTCAAGTC CAAACAAATG 
GGCACACGCC AGAGAGCCGT AGCCCTTTAC TTCATTGACA AGCTGGCTCT GAGAGCAGGT 
AATGAAAAGG AAGAGGGCGA GACGGCAGAC ACCGTGGGTT GTTGTTCACT CCGCGTGGAG 
CACATCACTT TACACGATGC ACTGGACGAC CAGAAGTGTG TGGTAGAGTT CGACTTCCTG 
GGTAAAGATT GCATCCGCTA CTACAACAAG GTTCCTGTGA CCAAAAGGGT TTTTAAAAAC 
CTCAAGCTTT TCATGGAAAA CAAGCAAGGA GGAGACGACC TCTTTGATCG ACTCAATACT 
GCTTCGCTGA ATAAACACCT GAGTTCCCTA ATGCCGGGAT TGACTGCAAA GGTTTTTAGG 
ACATACAATG CTTCCATAAC CCTGCAGCAA CAGCTGAAAG AGCTCAGCAA CATGAAGGAT 
AATGTGCCGG AGAAGTTGCT CTCCTATAAC AGGGCAAACC GTGCGGTTGC AGTTCTGTGT 
AACCATCAGC GGGCGCCGCC AAAGTCCTTC GATCAGTCCA TGGCCAACCT GCAGGCAAAG 
ATTGACAGCA GGAAGGAGCA GCTGGACTTG GCTAAGAAAG AGCTCAAGCA GGCCAAGAAA 
GAAGCCAAAG GGAGCTCAGA CAACAAGCTG CTGGTTGTGG TGGAGAAGAA GAAGAAAGCA 
GTCCAGAGGT GTGAGGAGCA GCTGCTGAAG ATGGAGGTTC AGGCTACAGA CAGAGAGGAG 
AACAAACAGA TCGCACTGAG CACCTCCAAA CTCAACTACC TGGATCCACG CATCAGTGTG 
GCCTGGTGTA AGAACATGGA GGTGCCGCTG GACAAAATTT ACAACAAAAC CTTGAGGGAT 
AAGTTTGCCT GGGCAGTCGA CATGACTGAA CTTGACTTTG TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zgc:173742 ( XM_002665326.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_002665372.2 Danio rerio zgc:173742 (zgc:173742), transcript variant X1, mRNA.

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CloneID	ODa23182
Clone ID Related Accession (Same CDS sequence)	XM_005169957.3 , XM_002665326.5 , XM_005169957.4 , XM_002665326.6
Accession Version	XM_002665326.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	DNA topoisomerase I, mitochondrial isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_002665326.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGAAAACG AGTTGAAAGA CGATCCATGC TCGAGTGAAG AGGCATTTTC AGAAAAGCCC 
AAGAAAATGA AAGCAAAGGC CAAAGGCAAG ACAAAGTCTC AGCGTGAAGG GAAGGAGAAA 
ACGCACAGGA AGATGAAAGC ATCGGAGGAG GAGATCATGG AAACTGAAGG CAGTGGTGAC 
AGAAGGTCTC ATGTAAATGT TAAGGAAGAG CCAAAGGAAG ATCCAGACTT TGTAACAGAA 
AAGAAAGGGA AAAAGAGGAA AAGAAAGGCG GATAATACAG ATGGACTCTT ATCTAGCGCC 
GAAGCACCTT TGCCAAAGAA GAAGAAGAAG AAAGTTGCAG ATGGACAGGA AAACGCTTCA 
ACAGCAAAGC AGAAAAAAAT GAAGGAAAAA AAAGAAAAGG TGAAAAAAGA GGAAGGAGAG 
CGTCCGGTCT CTACTAAGAA GAAAAAAACC AAGGAGGAGA TTGAAGCAGC CAGAGCTCTC 
AAGATAAAGA AGAAAGAGGA GGAAGAGAAA CAAAGATGGA GATGGTGGGA GGAAGAGAAG 
TATGAGAATG GAATTAAATG GAAGTTTCTA GAGCATAAAG GACCATATTT CCCTCCTGCG 
TATCAGCCCC TTCCTGATGA TGTCAAGTTT TACTATAATG GAGAGCCAGT GAAACTCAGC 
CTGCCAGCTG AGGAAGTGGC CCTTTACTTT GCTCAGATGC TGGATCACGA ATACACCACC 
AAAGAGGTCT TCCGCAATAA CTTCTTCAAG GACTGGAGGA AGGAAATGAC TCTAGAGGAG 
AGACAACTAA TCATGGATCT GAAAAAGTGT GATTTTGGGG AGCTCCATGC CATGCATAAG 
GAGAAGGTGG AAGCCAGGAA AAACCTGAGC AAAGAGGAGA AGTTGGTTAA TAAAGAGGCC 
AATCAAAAAA TAGTCGACGA ATATGGGTTC TGTGTGTTGG ACCACCATCG TGAACGCATT 
GGCAACTTTA AGATTGAACC TCCAGGTCTT TTCCGAGGCA GAGGAGACCA TCCTAAACAG 
GGAATGCTGA AGAAGAGGAT TCGACCTGAG GATGTTATCA TCAACTGCAG CAAAGAATCT 
CGTATCCCAG AAGCCCCAGC CGGTCACAAA TGGAAGGAAG TTCGTCATGA CAAAACCGTA 
ACATGGCTAG CAAGCTGGAC GGAGAATGTT CAAGGATCCT GTAAATATGT AATGCTCAAT 
GCCAACTCCA AACTTAAGGG TGAGAAGGAT TGGGAAAAGT ATGAAGTGGC CCGTAAACTG 
AAGACGTGTG TGGATGCCAT CCGAACCCAG TACCAGGAGG ACCTCAAGTC CAAACAAATG 
GGCACACGCC AGAGAGCCGT AGCCCTTTAC TTCATTGACA AGCTGGCTCT GAGAGCAGGT 
AATGAAAAGG AAGAGGGCGA GACGGCAGAC ACCGTGGGTT GTTGTTCACT CCGCGTGGAG 
CACATCACTT TACACGATGC ACTGGACGAC CAGAAGTGTG TGGTAGAGTT CGACTTCCTG 
GGTAAAGATT GCATCCGCTA CTACAACAAG GTTCCTGTGA CCAAAAGGGT TTTTAAAAAC 
CTCAAGCTTT TCATGGAAAA CAAGCAAGGA GGAGACGACC TCTTTGATCG ACTCAATACT 
GCTTCGCTGA ATAAACACCT GAGTTCCCTA ATGCCGGGAT TGACTGCAAA GGTTTTTAGG 
ACATACAATG CTTCCATAAC CCTGCAGCAA CAGCTGAAAG AGCTCAGCAA CATGAAGGAT 
AATGTGCCGG AGAAGTTGCT CTCCTATAAC AGGGCAAACC GTGCGGTTGC AGTTCTGTGT 
AACCATCAGC GGGCGCCGCC AAAGTCCTTC GATCAGTCCA TGGCCAACCT GCAGGCAAAG 
ATTGACAGCA GGAAGGAGCA GCTGGACTTG GCTAAGAAAG AGCTCAAGCA GGCCAAGAAA 
GAAGCCAAAG GGAGCTCAGA CAACAAGCTG CTGGTTGTGG TGGAGAAGAA GAAGAAAGCA 
GTCCAGAGGT GTGAGGAGCA GCTGCTGAAG ATGGAGGTTC AGGCTACAGA CAGAGAGGAG 
AACAAACAGA TCGCACTGAG CACCTCCAAA CTCAACTACC TGGATCCACG CATCAGTGTG 
GCCTGGTGTA AGAACATGGA GGTGCCGCTG GACAAAATTT ACAACAAAAC CTTGAGGGAT 
AAGTTTGCCT GGGCAGTCGA CATGACTGAA CTTGACTTTG TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002665372.2
CDS	436..2643
Translation	MENELKDDPCSSEEAFSEKPKKMKAKAKGKTKSQREGKEKTHRKMKASEEEIMETEGSGDRRSHVNVKEEPKEDPDFVTEKKGKKRKRKADNTDGLLSSAEAPLPKKKKKKVADGQENASTAKQKKMKEKKEKVKKEEGERPVSTKKKKTKEEIEAARALKIKKKEEEEKQRWRWWEEEKYENGIKWKFLEHKGPYFPPAYQPLPDDVKFYYNGEPVKLSLPAEEVALYFAQMLDHEYTTKEVFRNNFFKDWRKEMTLEERQLIMDLKKCDFGELHAMHKEKVEARKNLSKEEKLVNKEANQKIVDEYGFCVLDHHRERIGNFKIEPPGLFRGRGDHPKQGMLKKRIRPEDVIINCSKESRIPEAPAGHKWKEVRHDKTVTWLASWTENVQGSCKYVMLNANSKLKGEKDWEKYEVARKLKTCVDAIRTQYQEDLKSKQMGTRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHITLHDALDDQKCVVEFDFLGKDCIRYYNKVPVTKRVFKNLKLFMENKQGGDDLFDRLNTASLNKHLSSLMPGLTAKVFRTYNASITLQQQLKELSNMKDNVPEKLLSYNRANRAVAVLCNHQRAPPKSFDQSMANLQAKIDSRKEQLDLAKKELKQAKKEAKGSSDNKLLVVVEKKKKAVQRCEEQLLKMEVQATDREENKQIALSTSKLNYLDPRISVAWCKNMEVPLDKIYNKTLRDKFAWAVDMTELDFVF

Target ORF information:

RefSeq Version	XM_002665326.6
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zgc:173742 (zgc:173742), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002665326.6

ORF Insert Sequence:

ATGGAAAACG AGTTGAAAGA CGATCCATGC TCGAGTGAAG AGGCATTTTC AGAAAAGCCC 
AAGAAAATGA AAGCAAAGGC CAAAGGCAAG ACAAAGTCTC AGCGTGAAGG GAAGGAGAAA 
ACGCACAGGA AGATGAAAGC ATCGGAGGAG GAGATCATGG AAACTGAAGG CAGTGGTGAC 
AGAAGGTCTC ATGTAAATGT TAAGGAAGAG CCAAAGGAAG ATCCAGACTT TGTAACAGAA 
AAGAAAGGGA AAAAGAGGAA AAGAAAGGCG GATAATACAG ATGGACTCTT ATCTAGCGCC 
GAAGCACCTT TGCCAAAGAA GAAGAAGAAG AAAGTTGCAG ATGGACAGGA AAACGCTTCA 
ACAGCAAAGC AGAAAAAAAT GAAGGAAAAA AAAGAAAAGG TGAAAAAAGA GGAAGGAGAG 
CGTCCGGTCT CTACTAAGAA GAAAAAAACC AAGGAGGAGA TTGAAGCAGC CAGAGCTCTC 
AAGATAAAGA AGAAAGAGGA GGAAGAGAAA CAAAGATGGA GATGGTGGGA GGAAGAGAAG 
TATGAGAATG GAATTAAATG GAAGTTTCTA GAGCATAAAG GACCATATTT CCCTCCTGCG 
TATCAGCCCC TTCCTGATGA TGTCAAGTTT TACTATAATG GAGAGCCAGT GAAACTCAGC 
CTGCCAGCTG AGGAAGTGGC CCTTTACTTT GCTCAGATGC TGGATCACGA ATACACCACC 
AAAGAGGTCT TCCGCAATAA CTTCTTCAAG GACTGGAGGA AGGAAATGAC TCTAGAGGAG 
AGACAACTAA TCATGGATCT GAAAAAGTGT GATTTTGGGG AGCTCCATGC CATGCATAAG 
GAGAAGGTGG AAGCCAGGAA AAACCTGAGC AAAGAGGAGA AGTTGGTTAA TAAAGAGGCC 
AATCAAAAAA TAGTCGACGA ATATGGGTTC TGTGTGTTGG ACCACCATCG TGAACGCATT 
GGCAACTTTA AGATTGAACC TCCAGGTCTT TTCCGAGGCA GAGGAGACCA TCCTAAACAG 
GGAATGCTGA AGAAGAGGAT TCGACCTGAG GATGTTATCA TCAACTGCAG CAAAGAATCT 
CGTATCCCAG AAGCCCCAGC CGGTCACAAA TGGAAGGAAG TTCGTCATGA CAAAACCGTA 
ACATGGCTAG CAAGCTGGAC GGAGAATGTT CAAGGATCCT GTAAATATGT AATGCTCAAT 
GCCAACTCCA AACTTAAGGG TGAGAAGGAT TGGGAAAAGT ATGAAGTGGC CCGTAAACTG 
AAGACGTGTG TGGATGCCAT CCGAACCCAG TACCAGGAGG ACCTCAAGTC CAAACAAATG 
GGCACACGCC AGAGAGCCGT AGCCCTTTAC TTCATTGACA AGCTGGCTCT GAGAGCAGGT 
AATGAAAAGG AAGAGGGCGA GACGGCAGAC ACCGTGGGTT GTTGTTCACT CCGCGTGGAG 
CACATCACTT TACACGATGC ACTGGACGAC CAGAAGTGTG TGGTAGAGTT CGACTTCCTG 
GGTAAAGATT GCATCCGCTA CTACAACAAG GTTCCTGTGA CCAAAAGGGT TTTTAAAAAC 
CTCAAGCTTT TCATGGAAAA CAAGCAAGGA GGAGACGACC TCTTTGATCG ACTCAATACT 
GCTTCGCTGA ATAAACACCT GAGTTCCCTA ATGCCGGGAT TGACTGCAAA GGTTTTTAGG 
ACATACAATG CTTCCATAAC CCTGCAGCAA CAGCTGAAAG AGCTCAGCAA CATGAAGGAT 
AATGTGCCGG AGAAGTTGCT CTCCTATAAC AGGGCAAACC GTGCGGTTGC AGTTCTGTGT 
AACCATCAGC GGGCGCCGCC AAAGTCCTTC GATCAGTCCA TGGCCAACCT GCAGGCAAAG 
ATTGACAGCA GGAAGGAGCA GCTGGACTTG GCTAAGAAAG AGCTCAAGCA GGCCAAGAAA 
GAAGCCAAAG GGAGCTCAGA CAACAAGCTG CTGGTTGTGG TGGAGAAGAA GAAGAAAGCA 
GTCCAGAGGT GTGAGGAGCA GCTGCTGAAG ATGGAGGTTC AGGCTACAGA CAGAGAGGAG 
AACAAACAGA TCGCACTGAG CACCTCCAAA CTCAACTACC TGGATCCACG CATCAGTGTG 
GCCTGGTGTA AGAACATGGA GGTGCCGCTG GACAAAATTT ACAACAAAAC CTTGAGGGAT 
AAGTTTGCCT GGGCAGTCGA CATGACTGAA CTTGACTTTG TGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zgc:173742 ( XM_021473249.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328924.1 Danio rerio zgc:173742 (zgc:173742), transcript variant X4, mRNA.

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CloneID	ODa145991
Clone ID Related Accession (Same CDS sequence)	XM_021473249.1 , XM_021473248.1
Accession Version	XM_021473249.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	DNA topoisomerase I, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395014.1) and transcript sequence (GFIL01046394.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 1856 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGAAAACG AGTTGAAAGA CGATCCATGC TCGAGTGAAG AGGCATTTTC AGAAAAGCCC 
AAGAAAATGA AAGCAAAGGC CAAAGGCAAG ACAAAGTCTC AGCGTGAAGG GAAGGAGAAA 
ACGCACAGGA AGATGAAAGC ATCGGAGGAG GAGATCATGG AAACTGAAGG CAGTGGTGAC 
AGAAGGTCTC ATGTAAATGT TAAGGAAGAG CCAAAGGAAG ATCCAGACTT TGTAACAGAA 
AAGAAAGGGA AAAAGAGGAA AAGAAAGGCG GATAATACAG ATGGACTCTT ATCTAGCGCC 
GAAGCACCTT TGCCAAAGAA GAAGAAGAAG AAAGTTGCAG ATGGACAGGA AAACGCTTCA 
ACAGCAAAGC AGAAAAAAAT GAAGGAAAAA AAAGAAAAGG TGAAAAAAGA GGAAGGAGAG 
CGTCCGGTCT CTACTAAGAA GAAAAAAACC AAGGAGGAGA TTGAAGCAGC CAGAGCTCTC 
AAGATAAAGA AGAAAGAGGA GGAAGAGAAA CAAAGATGGA GATGGTGGGA GGAAGAGAAG 
TATGAGAATG GAATTAAATG GAAGTTTCTA GAGCATAAAG GACCATATTT CCCTCCTGCG 
TATCAGCCCC TTCCTGATGA TGTCAAGTTT TACTATAATG GAGAGCCAGT GAAACTCAGC 
CTGCCAGCTG AGGAAGTGGC CCTTTACTTT GCTCAGATGC TGGATCACGA ATACACCACC 
AAAGAGGTCT TCCGCAATAA CTTCTTCAAG GACTGGAGGA AGGAAATGAC TCTAGAGGAG 
AGACAACTAA TCATGGATCT GAAAAAGTGT GATTTTGGGG AGCTCCATGC CATGCATAAG 
GAGAAGGTGG AAGCCAGGAA AAACCTGAGC AAAGAGGAGA AGTTGGTTAA TAAAGAGGCC 
AATCAAAAAA TAGTCGACGA ATATGGGTTC TGTGTGTTGG ACCACCATCG TGAACGCATT 
GGCAACTTTA AGATTGAACC TCCAGGTCTT TTCCGAGGCA GAGGAGACCA TCCTAAACAG 
GGAATGCTGA AGAAGAGGAT TCGACCTGAG GATGTTATCA TCAACTGCAG CAAAGAATCT 
CGTATCCCAG AAGCCCCAGC CGGTCACAAA TGGAAGGAAG TTCGTCATGA CAAAACCGTA 
ACATGGCTAG CAAGCTGGAC GGAGAATGTT CAAGGATCCT GTAAATATGT AATGCTCAAT 
GCCAACTCCA AACTTAAGGG TGAGAAGGAT TGGGAAAAGT ATGAAGTGGC CCGTAAACTG 
AAGACGTGTG TGGATGCCAT CCGAACCCAG TACCAGGAGG ACCTCAAGTC CAAACAAATG 
GGCACACGCC AGAGAGCCGT AGCCCTTTAC TTCATTGACA AGCTGGCTCT GAGAGCAGGT 
AATGAAAAGG AAGAGGGCGA GACGGCAGAC ACCGTGGGTT GTTGTTCACT CCGCGTGGAG 
CACATCACTT TACACGATGC ACTGGACGAC CAGAAGTGTG TGGTAGAGTT CGACTTCCTG 
GGTAAAGATT GCATCCGCTA CTACAACAAG GTTCCTGTGA CCAAAAGGGT TTTTAAAAAC 
CTCAAGCTTT TCATGGAAAA CAAGCAAGGA GGAGACGACC TCTTTGATCG ACTCAATACT 
GCTTCGCTGA ATAAACACCT GAGTTCCCTA ATGCCGGGAT TGACTGCAAA GGTTTTTAGG 
ACATACAATG CTTCCATAAC CCTGCAGCAA CAGCTGAAAG AGCTCAGCAA CATGAAGGAT 
AATGTGCCGG AGAAGTTGCT CTCCTATAAC AGGGCAAACC GTGCGGTTGC AGTTCTGTGT 
AACCATCAGC GGGCGCCGCC AAAGTCCTTC GATCAGTCCA TGGCCAACCT GCAGGCAAAG 
ATTGACAGCA GGAAGGAGCA GCTGGACTTG GCTAAGAAAG AGCTCAAGCA GGCCAAGAAA 
GAAGCCAAAG GGAGCTCAGA CAACAAGCTG CTGGTTGTGG TGGAGAAGAA GAAGAAAGCA 
GTCCAGAGGT GTGAGGAGCA GCTGCTGAAG ATGGAGGTTC AGGCTACAGA CAGAGAGGAG 
AACAAACAGA TCGCACTGAG CACCTCCAAA CTCAACTACC TGGATCCACG CATCTCTGTA 
GCCTGGTGTA AGAAGTTCGG CATCCCGATA GAGAAGATCT ACAACAAAAC TCAGCGTGAG 
AAATTTGCCT GGGCGATTGA CATGGCTGAA AAAGACTATG AATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328924.1
CDS	433..2640
Translation	MENELKDDPCSSEEAFSEKPKKMKAKAKGKTKSQREGKEKTHRKMKASEEEIMETEGSGDRRSHVNVKEEPKEDPDFVTEKKGKKRKRKADNTDGLLSSAEAPLPKKKKKKVADGQENASTAKQKKMKEKKEKVKKEEGERPVSTKKKKTKEEIEAARALKIKKKEEEEKQRWRWWEEEKYENGIKWKFLEHKGPYFPPAYQPLPDDVKFYYNGEPVKLSLPAEEVALYFAQMLDHEYTTKEVFRNNFFKDWRKEMTLEERQLIMDLKKCDFGELHAMHKEKVEARKNLSKEEKLVNKEANQKIVDEYGFCVLDHHRERIGNFKIEPPGLFRGRGDHPKQGMLKKRIRPEDVIINCSKESRIPEAPAGHKWKEVRHDKTVTWLASWTENVQGSCKYVMLNANSKLKGEKDWEKYEVARKLKTCVDAIRTQYQEDLKSKQMGTRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHITLHDALDDQKCVVEFDFLGKDCIRYYNKVPVTKRVFKNLKLFMENKQGGDDLFDRLNTASLNKHLSSLMPGLTAKVFRTYNASITLQQQLKELSNMKDNVPEKLLSYNRANRAVAVLCNHQRAPPKSFDQSMANLQAKIDSRKEQLDLAKKELKQAKKEAKGSSDNKLLVVVEKKKKAVQRCEEQLLKMEVQATDREENKQIALSTSKLNYLDPRISVAWCKKFGIPIEKIYNKTQREKFAWAIDMAEKDYEF

Target ORF information:

RefSeq Version	XM_021473249.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zgc:173742 (zgc:173742), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473249.1

ORF Insert Sequence:

ATGGAAAACG AGTTGAAAGA CGATCCATGC TCGAGTGAAG AGGCATTTTC AGAAAAGCCC 
AAGAAAATGA AAGCAAAGGC CAAAGGCAAG ACAAAGTCTC AGCGTGAAGG GAAGGAGAAA 
ACGCACAGGA AGATGAAAGC ATCGGAGGAG GAGATCATGG AAACTGAAGG CAGTGGTGAC 
AGAAGGTCTC ATGTAAATGT TAAGGAAGAG CCAAAGGAAG ATCCAGACTT TGTAACAGAA 
AAGAAAGGGA AAAAGAGGAA AAGAAAGGCG GATAATACAG ATGGACTCTT ATCTAGCGCC 
GAAGCACCTT TGCCAAAGAA GAAGAAGAAG AAAGTTGCAG ATGGACAGGA AAACGCTTCA 
ACAGCAAAGC AGAAAAAAAT GAAGGAAAAA AAAGAAAAGG TGAAAAAAGA GGAAGGAGAG 
CGTCCGGTCT CTACTAAGAA GAAAAAAACC AAGGAGGAGA TTGAAGCAGC CAGAGCTCTC 
AAGATAAAGA AGAAAGAGGA GGAAGAGAAA CAAAGATGGA GATGGTGGGA GGAAGAGAAG 
TATGAGAATG GAATTAAATG GAAGTTTCTA GAGCATAAAG GACCATATTT CCCTCCTGCG 
TATCAGCCCC TTCCTGATGA TGTCAAGTTT TACTATAATG GAGAGCCAGT GAAACTCAGC 
CTGCCAGCTG AGGAAGTGGC CCTTTACTTT GCTCAGATGC TGGATCACGA ATACACCACC 
AAAGAGGTCT TCCGCAATAA CTTCTTCAAG GACTGGAGGA AGGAAATGAC TCTAGAGGAG 
AGACAACTAA TCATGGATCT GAAAAAGTGT GATTTTGGGG AGCTCCATGC CATGCATAAG 
GAGAAGGTGG AAGCCAGGAA AAACCTGAGC AAAGAGGAGA AGTTGGTTAA TAAAGAGGCC 
AATCAAAAAA TAGTCGACGA ATATGGGTTC TGTGTGTTGG ACCACCATCG TGAACGCATT 
GGCAACTTTA AGATTGAACC TCCAGGTCTT TTCCGAGGCA GAGGAGACCA TCCTAAACAG 
GGAATGCTGA AGAAGAGGAT TCGACCTGAG GATGTTATCA TCAACTGCAG CAAAGAATCT 
CGTATCCCAG AAGCCCCAGC CGGTCACAAA TGGAAGGAAG TTCGTCATGA CAAAACCGTA 
ACATGGCTAG CAAGCTGGAC GGAGAATGTT CAAGGATCCT GTAAATATGT AATGCTCAAT 
GCCAACTCCA AACTTAAGGG TGAGAAGGAT TGGGAAAAGT ATGAAGTGGC CCGTAAACTG 
AAGACGTGTG TGGATGCCAT CCGAACCCAG TACCAGGAGG ACCTCAAGTC CAAACAAATG 
GGCACACGCC AGAGAGCCGT AGCCCTTTAC TTCATTGACA AGCTGGCTCT GAGAGCAGGT 
AATGAAAAGG AAGAGGGCGA GACGGCAGAC ACCGTGGGTT GTTGTTCACT CCGCGTGGAG 
CACATCACTT TACACGATGC ACTGGACGAC CAGAAGTGTG TGGTAGAGTT CGACTTCCTG 
GGTAAAGATT GCATCCGCTA CTACAACAAG GTTCCTGTGA CCAAAAGGGT TTTTAAAAAC 
CTCAAGCTTT TCATGGAAAA CAAGCAAGGA GGAGACGACC TCTTTGATCG ACTCAATACT 
GCTTCGCTGA ATAAACACCT GAGTTCCCTA ATGCCGGGAT TGACTGCAAA GGTTTTTAGG 
ACATACAATG CTTCCATAAC CCTGCAGCAA CAGCTGAAAG AGCTCAGCAA CATGAAGGAT 
AATGTGCCGG AGAAGTTGCT CTCCTATAAC AGGGCAAACC GTGCGGTTGC AGTTCTGTGT 
AACCATCAGC GGGCGCCGCC AAAGTCCTTC GATCAGTCCA TGGCCAACCT GCAGGCAAAG 
ATTGACAGCA GGAAGGAGCA GCTGGACTTG GCTAAGAAAG AGCTCAAGCA GGCCAAGAAA 
GAAGCCAAAG GGAGCTCAGA CAACAAGCTG CTGGTTGTGG TGGAGAAGAA GAAGAAAGCA 
GTCCAGAGGT GTGAGGAGCA GCTGCTGAAG ATGGAGGTTC AGGCTACAGA CAGAGAGGAG 
AACAAACAGA TCGCACTGAG CACCTCCAAA CTCAACTACC TGGATCCACG CATCTCTGTA 
GCCTGGTGTA AGAAGTTCGG CATCCCGATA GAGAAGATCT ACAACAAAAC TCAGCGTGAG 
AAATTTGCCT GGGCGATTGA CATGGCTGAA AAAGACTATG AATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

zgc:173742 ( XM_021473248.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021328923.1 Danio rerio zgc:173742 (zgc:173742), transcript variant X3, mRNA.

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CloneID	ODa145991
Clone ID Related Accession (Same CDS sequence)	XM_021473249.1 , XM_021473248.1
Accession Version	XM_021473248.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2208bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	DNA topoisomerase I, mitochondrial isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395014.1) and transcript sequence (GFIL01046394.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 1856 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGAAAACG AGTTGAAAGA CGATCCATGC TCGAGTGAAG AGGCATTTTC AGAAAAGCCC 
AAGAAAATGA AAGCAAAGGC CAAAGGCAAG ACAAAGTCTC AGCGTGAAGG GAAGGAGAAA 
ACGCACAGGA AGATGAAAGC ATCGGAGGAG GAGATCATGG AAACTGAAGG CAGTGGTGAC 
AGAAGGTCTC ATGTAAATGT TAAGGAAGAG CCAAAGGAAG ATCCAGACTT TGTAACAGAA 
AAGAAAGGGA AAAAGAGGAA AAGAAAGGCG GATAATACAG ATGGACTCTT ATCTAGCGCC 
GAAGCACCTT TGCCAAAGAA GAAGAAGAAG AAAGTTGCAG ATGGACAGGA AAACGCTTCA 
ACAGCAAAGC AGAAAAAAAT GAAGGAAAAA AAAGAAAAGG TGAAAAAAGA GGAAGGAGAG 
CGTCCGGTCT CTACTAAGAA GAAAAAAACC AAGGAGGAGA TTGAAGCAGC CAGAGCTCTC 
AAGATAAAGA AGAAAGAGGA GGAAGAGAAA CAAAGATGGA GATGGTGGGA GGAAGAGAAG 
TATGAGAATG GAATTAAATG GAAGTTTCTA GAGCATAAAG GACCATATTT CCCTCCTGCG 
TATCAGCCCC TTCCTGATGA TGTCAAGTTT TACTATAATG GAGAGCCAGT GAAACTCAGC 
CTGCCAGCTG AGGAAGTGGC CCTTTACTTT GCTCAGATGC TGGATCACGA ATACACCACC 
AAAGAGGTCT TCCGCAATAA CTTCTTCAAG GACTGGAGGA AGGAAATGAC TCTAGAGGAG 
AGACAACTAA TCATGGATCT GAAAAAGTGT GATTTTGGGG AGCTCCATGC CATGCATAAG 
GAGAAGGTGG AAGCCAGGAA AAACCTGAGC AAAGAGGAGA AGTTGGTTAA TAAAGAGGCC 
AATCAAAAAA TAGTCGACGA ATATGGGTTC TGTGTGTTGG ACCACCATCG TGAACGCATT 
GGCAACTTTA AGATTGAACC TCCAGGTCTT TTCCGAGGCA GAGGAGACCA TCCTAAACAG 
GGAATGCTGA AGAAGAGGAT TCGACCTGAG GATGTTATCA TCAACTGCAG CAAAGAATCT 
CGTATCCCAG AAGCCCCAGC CGGTCACAAA TGGAAGGAAG TTCGTCATGA CAAAACCGTA 
ACATGGCTAG CAAGCTGGAC GGAGAATGTT CAAGGATCCT GTAAATATGT AATGCTCAAT 
GCCAACTCCA AACTTAAGGG TGAGAAGGAT TGGGAAAAGT ATGAAGTGGC CCGTAAACTG 
AAGACGTGTG TGGATGCCAT CCGAACCCAG TACCAGGAGG ACCTCAAGTC CAAACAAATG 
GGCACACGCC AGAGAGCCGT AGCCCTTTAC TTCATTGACA AGCTGGCTCT GAGAGCAGGT 
AATGAAAAGG AAGAGGGCGA GACGGCAGAC ACCGTGGGTT GTTGTTCACT CCGCGTGGAG 
CACATCACTT TACACGATGC ACTGGACGAC CAGAAGTGTG TGGTAGAGTT CGACTTCCTG 
GGTAAAGATT GCATCCGCTA CTACAACAAG GTTCCTGTGA CCAAAAGGGT TTTTAAAAAC 
CTCAAGCTTT TCATGGAAAA CAAGCAAGGA GGAGACGACC TCTTTGATCG ACTCAATACT 
GCTTCGCTGA ATAAACACCT GAGTTCCCTA ATGCCGGGAT TGACTGCAAA GGTTTTTAGG 
ACATACAATG CTTCCATAAC CCTGCAGCAA CAGCTGAAAG AGCTCAGCAA CATGAAGGAT 
AATGTGCCGG AGAAGTTGCT CTCCTATAAC AGGGCAAACC GTGCGGTTGC AGTTCTGTGT 
AACCATCAGC GGGCGCCGCC AAAGTCCTTC GATCAGTCCA TGGCCAACCT GCAGGCAAAG 
ATTGACAGCA GGAAGGAGCA GCTGGACTTG GCTAAGAAAG AGCTCAAGCA GGCCAAGAAA 
GAAGCCAAAG GGAGCTCAGA CAACAAGCTG CTGGTTGTGG TGGAGAAGAA GAAGAAAGCA 
GTCCAGAGGT GTGAGGAGCA GCTGCTGAAG ATGGAGGTTC AGGCTACAGA CAGAGAGGAG 
AACAAACAGA TCGCACTGAG CACCTCCAAA CTCAACTACC TGGATCCACG CATCTCTGTA 
GCCTGGTGTA AGAAGTTCGG CATCCCGATA GAGAAGATCT ACAACAAAAC TCAGCGTGAG 
AAATTTGCCT GGGCGATTGA CATGGCTGAA AAAGACTATG AATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021328923.1
CDS	448..2655
Translation	MENELKDDPCSSEEAFSEKPKKMKAKAKGKTKSQREGKEKTHRKMKASEEEIMETEGSGDRRSHVNVKEEPKEDPDFVTEKKGKKRKRKADNTDGLLSSAEAPLPKKKKKKVADGQENASTAKQKKMKEKKEKVKKEEGERPVSTKKKKTKEEIEAARALKIKKKEEEEKQRWRWWEEEKYENGIKWKFLEHKGPYFPPAYQPLPDDVKFYYNGEPVKLSLPAEEVALYFAQMLDHEYTTKEVFRNNFFKDWRKEMTLEERQLIMDLKKCDFGELHAMHKEKVEARKNLSKEEKLVNKEANQKIVDEYGFCVLDHHRERIGNFKIEPPGLFRGRGDHPKQGMLKKRIRPEDVIINCSKESRIPEAPAGHKWKEVRHDKTVTWLASWTENVQGSCKYVMLNANSKLKGEKDWEKYEVARKLKTCVDAIRTQYQEDLKSKQMGTRQRAVALYFIDKLALRAGNEKEEGETADTVGCCSLRVEHITLHDALDDQKCVVEFDFLGKDCIRYYNKVPVTKRVFKNLKLFMENKQGGDDLFDRLNTASLNKHLSSLMPGLTAKVFRTYNASITLQQQLKELSNMKDNVPEKLLSYNRANRAVAVLCNHQRAPPKSFDQSMANLQAKIDSRKEQLDLAKKELKQAKKEAKGSSDNKLLVVVEKKKKAVQRCEEQLLKMEVQATDREENKQIALSTSKLNYLDPRISVAWCKKFGIPIEKIYNKTQREKFAWAIDMAEKDYEF

Target ORF information:

RefSeq Version	XM_021473248.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zgc:173742 (zgc:173742), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021473248.1

ORF Insert Sequence:

ATGGAAAACG AGTTGAAAGA CGATCCATGC TCGAGTGAAG AGGCATTTTC AGAAAAGCCC 
AAGAAAATGA AAGCAAAGGC CAAAGGCAAG ACAAAGTCTC AGCGTGAAGG GAAGGAGAAA 
ACGCACAGGA AGATGAAAGC ATCGGAGGAG GAGATCATGG AAACTGAAGG CAGTGGTGAC 
AGAAGGTCTC ATGTAAATGT TAAGGAAGAG CCAAAGGAAG ATCCAGACTT TGTAACAGAA 
AAGAAAGGGA AAAAGAGGAA AAGAAAGGCG GATAATACAG ATGGACTCTT ATCTAGCGCC 
GAAGCACCTT TGCCAAAGAA GAAGAAGAAG AAAGTTGCAG ATGGACAGGA AAACGCTTCA 
ACAGCAAAGC AGAAAAAAAT GAAGGAAAAA AAAGAAAAGG TGAAAAAAGA GGAAGGAGAG 
CGTCCGGTCT CTACTAAGAA GAAAAAAACC AAGGAGGAGA TTGAAGCAGC CAGAGCTCTC 
AAGATAAAGA AGAAAGAGGA GGAAGAGAAA CAAAGATGGA GATGGTGGGA GGAAGAGAAG 
TATGAGAATG GAATTAAATG GAAGTTTCTA GAGCATAAAG GACCATATTT CCCTCCTGCG 
TATCAGCCCC TTCCTGATGA TGTCAAGTTT TACTATAATG GAGAGCCAGT GAAACTCAGC 
CTGCCAGCTG AGGAAGTGGC CCTTTACTTT GCTCAGATGC TGGATCACGA ATACACCACC 
AAAGAGGTCT TCCGCAATAA CTTCTTCAAG GACTGGAGGA AGGAAATGAC TCTAGAGGAG 
AGACAACTAA TCATGGATCT GAAAAAGTGT GATTTTGGGG AGCTCCATGC CATGCATAAG 
GAGAAGGTGG AAGCCAGGAA AAACCTGAGC AAAGAGGAGA AGTTGGTTAA TAAAGAGGCC 
AATCAAAAAA TAGTCGACGA ATATGGGTTC TGTGTGTTGG ACCACCATCG TGAACGCATT 
GGCAACTTTA AGATTGAACC TCCAGGTCTT TTCCGAGGCA GAGGAGACCA TCCTAAACAG 
GGAATGCTGA AGAAGAGGAT TCGACCTGAG GATGTTATCA TCAACTGCAG CAAAGAATCT 
CGTATCCCAG AAGCCCCAGC CGGTCACAAA TGGAAGGAAG TTCGTCATGA CAAAACCGTA 
ACATGGCTAG CAAGCTGGAC GGAGAATGTT CAAGGATCCT GTAAATATGT AATGCTCAAT 
GCCAACTCCA AACTTAAGGG TGAGAAGGAT TGGGAAAAGT ATGAAGTGGC CCGTAAACTG 
AAGACGTGTG TGGATGCCAT CCGAACCCAG TACCAGGAGG ACCTCAAGTC CAAACAAATG 
GGCACACGCC AGAGAGCCGT AGCCCTTTAC TTCATTGACA AGCTGGCTCT GAGAGCAGGT 
AATGAAAAGG AAGAGGGCGA GACGGCAGAC ACCGTGGGTT GTTGTTCACT CCGCGTGGAG 
CACATCACTT TACACGATGC ACTGGACGAC CAGAAGTGTG TGGTAGAGTT CGACTTCCTG 
GGTAAAGATT GCATCCGCTA CTACAACAAG GTTCCTGTGA CCAAAAGGGT TTTTAAAAAC 
CTCAAGCTTT TCATGGAAAA CAAGCAAGGA GGAGACGACC TCTTTGATCG ACTCAATACT 
GCTTCGCTGA ATAAACACCT GAGTTCCCTA ATGCCGGGAT TGACTGCAAA GGTTTTTAGG 
ACATACAATG CTTCCATAAC CCTGCAGCAA CAGCTGAAAG AGCTCAGCAA CATGAAGGAT 
AATGTGCCGG AGAAGTTGCT CTCCTATAAC AGGGCAAACC GTGCGGTTGC AGTTCTGTGT 
AACCATCAGC GGGCGCCGCC AAAGTCCTTC GATCAGTCCA TGGCCAACCT GCAGGCAAAG 
ATTGACAGCA GGAAGGAGCA GCTGGACTTG GCTAAGAAAG AGCTCAAGCA GGCCAAGAAA 
GAAGCCAAAG GGAGCTCAGA CAACAAGCTG CTGGTTGTGG TGGAGAAGAA GAAGAAAGCA 
GTCCAGAGGT GTGAGGAGCA GCTGCTGAAG ATGGAGGTTC AGGCTACAGA CAGAGAGGAG 
AACAAACAGA TCGCACTGAG CACCTCCAAA CTCAACTACC TGGATCCACG CATCTCTGTA 
GCCTGGTGTA AGAAGTTCGG CATCCCGATA GAGAAGATCT ACAACAAAAC TCAGCGTGAG 
AAATTTGCCT GGGCGATTGA CATGGCTGAA AAAGACTATG AATTTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

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zgc:173742 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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