brf1a cDNA ORF clone, Danio rerio(zebrafish)

The following brf1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the brf1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa02697 NM_199898.1
Latest version!
Danio rerio BRF1 RNA polymerase III transcription initiation factor subunit a (brf1a), mRNA. pcDNA3.1-C-(k)DYK or customized vector 7-9 $342.30
$489.00
ODa54671 XM_017358699.2
Latest version!
Danio rerio BRF1, RNA polymerase III transcription initiation factor a (brf1a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
View Old Accession Versions >>
ODa54671 XM_017358699.1 Danio rerio BRF1, RNA polymerase III transcription initiation factor a (brf1a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $342.30
$489.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa02697
    Clone ID Related Accession (Same CDS sequence) NM_199898.1
    Accession Version NM_199898.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1986bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-04-07
    Organism Danio rerio(zebrafish)
    Product BRF1 RNA polymerase III transcription initiation factor subunit a
    Comment Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BC046877.1. On Jul 6, 2005 this sequence version replaced XM_683634.1. ##Evidence-Data-START## Transcript exon combination :: BC046877.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168447, SAMEA2168448 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGGGCGGT GCAAGACCTG CGGTGGGACA GACATCGATG TGGACCAGGC TCGGGGTGTT 
    GCGGTGTGCA TGGGCTGCGG CTCTGTGCTG GAGGACAACA TCATCGTCTC TGAGGTCACC
    TTTGTGGAGA GTGGCGGCGG CGGATCGTCA GCTGTTGGCC AGTTCGTTGC TGGCGATGCA
    TCTGGAAATG TGCCTTCACT GGGAGGAAAT TTCCAAACTA GTGTCGGGAG AGAGTCCAGA
    GCTGCCACTC TGCAGAATGC GAAGCGACAG ATAAATCACC TTGGTCATCA GCTTCAGATG
    AATCAGCACT GTCTGGACAC TGCCTTCAAC TTTTATAAGA TGGCTCTCTC TAAACATCTG
    ACCAAAGGAC GCAAATCAAC TCATGTCATC GCCGCCTGCC TCTACCTTGT CTGCCGAACC
    GAGGGAACAC CTCATATGCT GCTTGATTTG AGTGACCTCT TGCAGGTGAA CGTGTACGTT
    TTGGGAAAAA CATTCCTTGT TTTAGCCAGA GAGCTGTGTA TCAACGCACC AGCTATCGAT
    CCATGTCTTT ATATCCCACG CTTTGCACAA CTGCTGGAAT TTGGAGAGAA GAGCCATGAG
    GTTTCTATGA CGGCACTGCG TCTCCTGCAG AGGATGAAGA GAGACTGGAT GCACACGGGA
    CGAAGACCTT CAGGCCTCTG CGGAGCAGCT CTCCTGGTTG CGGCCCGTAT GCACGAGTTC
    CGTCGCACAA TTAAAGAAGT GATCAGTGTG GTGAAAGTCT GCGAGGCCAC GCTAAGGAAA
    AGGTTGTATG AATTTGAGGA CACGCCCACC AGTGAGCTGA CTATTGAAGA GTTCATGAAG
    ACTGATCTGG AGCAGGAGTG CGACCCGCCT TCATTCGCAG CAGGACTCAG GAAGCAAAAG
    CTAAAACAGA TTGAACAGGA GCTTGCGAAG AAAGTTGATG ATATTGAAGG TGAAATATGT
    GGCTATCAGG ATGAGATTGA AGTTGAGTTG GAAAGCTGTA GGCCCAAAGC TAGGGGGATT
    TATGCATCCT GTTCCAGAGA AGATGATGTG GTTTCCTTGG CATCCTCCAG TCACCTTGAT
    GGAGAAGATG ATGAACTGAG GGCAGCTGCA TCTCATCTGT ATGGGGAGAA TGAAGAGCAG
    GAGCAAGATG GAGAGAGAGT TTCTCTAACC AAACGGCCCT CGTTATCATT GTTGCTTGGA
    GCGCTGCCCA CTTCGGCCAG TTTGGGCCTG TCCGAGTCCA TCACCAAAAT GGGAGAGGAG
    AAGGAGAACG ATGTGGAGGC AGAAGGCGGT GAGCTAGACC TGAGCGGTAT TGACGAAGAT
    GAGATCGATC GGTATATCCT GAATGACAAA GAAGTTAAGG TGAAAACTGA ACTGTGGATG
    ATTCAAAACG CAGACTACCT CAAAGAGCAA AAAGAAAAGG AAGAAAGAAT AGCAAAAGAA
    AAGGAGGAAG GTACATATAA AGAGCGGAAG CCTAGAAAGT CAACACGAAG GCGTGAGCCC
    ATCAATGCCA ACACTGCAGA CGAAGCCATT GAAAAGATGT TAGAACAGAA ACGCATCTCC
    ACCAAAATAA ACTATGATGT TCTGAAAGAC TTGAACAAAT CCAGCCCAAA AAGTCCCACT
    CGCCAGCCTG AAGAAGCTTC AGCTGGAGCC AAAAGAACCC CCGCGGCCCG CCAACGGAAA
    CCCCAACTGA CTTCACCCGA TATTGGCAAA ACTGTCAGCA GCTTTGGCAA GAGGTTACAG
    CCCCTGGTGC GTGCGCAGCC CTCTAAGAAG CTGGCCCTTG ATCAGATAGG CTCTTCTGTT
    CCAGTGGCGA CTCCTGCTGT CTCAGCTCAG ACTGCTGTTG TTGTAGAGAG CGGACCAGTG
    GCTTATGAAG AGCATGCAGA TGAGGAAGAA GAGGATGAGG AGGATGAGCA CTGTGTCAGT
    GCCATGCAAT TAATGGGCGG CACTAGTGAT TATGGGTATG AATTAGATGA TGATGATGGC
    TTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_956192.1
    CDS194..2179
    Translation

    Target ORF information:

    RefSeq Version NM_199898.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio BRF1 RNA polymerase III transcription initiation factor subunit a (brf1a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_199898.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGGGCGGT GCAAGACCTG CGGTGGGACA GACATCGATG TGGACCAGGC TCGGGGTGTT 
    GCGGTGTGCA TGGGCTGCGG CTCTGTGCTG GAGGACAACA TCATCGTCTC TGAGGTCACC
    TTTGTGGAGA GTGGCGGCGG CGGATCGTCA GCTGTTGGCC AGTTCGTTGC TGGCGATGCA
    TCTGGAAATG TGCCTTCACT GGGAGGAAAT TTCCAAACTA GTGTCGGGAG AGAGTCCAGA
    GCTGCCACTC TGCAGAATGC GAAGCGACAG ATAAATCACC TTGGTCATCA GCTTCAGATG
    AATCAGCACT GTCTGGACAC TGCCTTCAAC TTTTATAAGA TGGCTCTCTC TAAACATCTG
    ACCAAAGGAC GCAAATCAAC TCATGTCATC GCCGCCTGCC TCTACCTTGT CTGCCGAACC
    GAGGGAACAC CTCATATGCT GCTTGATTTG AGTGACCTCT TGCAGGTGAA CGTGTACGTT
    TTGGGAAAAA CATTCCTTGT TTTAGCCAGA GAGCTGTGTA TCAACGCACC AGCTATCGAT
    CCATGTCTTT ATATCCCACG CTTTGCACAA CTGCTGGAAT TTGGAGAGAA GAGCCATGAG
    GTTTCTATGA CGGCACTGCG TCTCCTGCAG AGGATGAAGA GAGACTGGAT GCACACGGGA
    CGAAGACCTT CAGGCCTCTG CGGAGCAGCT CTCCTGGTTG CGGCCCGTAT GCACGAGTTC
    CGTCGCACAA TTAAAGAAGT GATCAGTGTG GTGAAAGTCT GCGAGGCCAC GCTAAGGAAA
    AGGTTGTATG AATTTGAGGA CACGCCCACC AGTGAGCTGA CTATTGAAGA GTTCATGAAG
    ACTGATCTGG AGCAGGAGTG CGACCCGCCT TCATTCGCAG CAGGACTCAG GAAGCAAAAG
    CTAAAACAGA TTGAACAGGA GCTTGCGAAG AAAGTTGATG ATATTGAAGG TGAAATATGT
    GGCTATCAGG ATGAGATTGA AGTTGAGTTG GAAAGCTGTA GGCCCAAAGC TAGGGGGATT
    TATGCATCCT GTTCCAGAGA AGATGATGTG GTTTCCTTGG CATCCTCCAG TCACCTTGAT
    GGAGAAGATG ATGAACTGAG GGCAGCTGCA TCTCATCTGT ATGGGGAGAA TGAAGAGCAG
    GAGCAAGATG GAGAGAGAGT TTCTCTAACC AAACGGCCCT CGTTATCATT GTTGCTTGGA
    GCGCTGCCCA CTTCGGCCAG TTTGGGCCTG TCCGAGTCCA TCACCAAAAT GGGAGAGGAG
    AAGGAGAACG ATGTGGAGGC AGAAGGCGGT GAGCTAGACC TGAGCGGTAT TGACGAAGAT
    GAGATCGATC GGTATATCCT GAATGACAAA GAAGTTAAGG TGAAAACTGA ACTGTGGATG
    ATTCAAAACG CAGACTACCT CAAAGAGCAA AAAGAAAAGG AAGAAAGAAT AGCAAAAGAA
    AAGGAGGAAG GTACATATAA AGAGCGGAAG CCTAGAAAGT CAACACGAAG GCGTGAGCCC
    ATCAATGCCA ACACTGCAGA CGAAGCCATT GAAAAGATGT TAGAACAGAA ACGCATCTCC
    ACCAAAATAA ACTATGATGT TCTGAAAGAC TTGAACAAAT CCAGCCCAAA AAGTCCCACT
    CGCCAGCCTG AAGAAGCTTC AGCTGGAGCC AAAAGAACCC CCGCGGCCCG CCAACGGAAA
    CCCCAACTGA CTTCACCCGA TATTGGCAAA ACTGTCAGCA GCTTTGGCAA GAGGTTACAG
    CCCCTGGTGC GTGCGCAGCC CTCTAAGAAG CTGGCCCTTG ATCAGATAGG CTCTTCTGTT
    CCAGTGGCGA CTCCTGCTGT CTCAGCTCAG ACTGCTGTTG TTGTAGAGAG CGGACCAGTG
    GCTTATGAAG AGCATGCAGA TGAGGAAGAA GAGGATGAGG AGGATGAGCA CTGTGTCAGT
    GCCATGCAAT TAATGGGCGG CACTAGTGAT TATGGGTATG AATTAGATGA TGATGATGGC
    TTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54671
    Clone ID Related Accession (Same CDS sequence) XM_017358699.2 , XM_017358699.1
    Accession Version XM_017358699.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1986bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product BRF1, RNA polymerase III transcription initiation factor a isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358699.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGGGCGGT GCAAGACCTG CGGTGGGACA GACATCGATG TGGACCAGGC TCGGGGTGTT 
    GCGGTGTGCA TGGGCTGTGG CTCTGTGCTG GAGGACAACA TCATCGTCTC TGAGGTCACC
    TTTGTGGAGA GTGGCGGCGG CGGATCGTCA GCTGTTGGCC AGTTCGTCGC TGGCGATGCA
    TCTGGAAATG TGCCTTCACT GGGAGGAAAT TTCCAAACTA GTGTCGGGAG AGAGTCCAGA
    GCTGCCACTC TGCAGAATGC GAAGCGACAG ATAAATCACC TTGGTCATCA GCTTCAGATG
    AATCAGCACT GTCTGGACAC TGCCTTCAAC TTTTATAAGA TGGCTCTCTC TAAACATCTG
    ACCAAAGGAC GCAAATCAAC TCATGTCATC GCCGCCTGCC TCTACCTCGT CTGCCGAACC
    GAGGGAACAC CTCATATGCT GCTTGATTTG AGTGACCTCT TGCAGGTGAA CGTGTACGTT
    TTGGGAAAAA CATTCCTTGT TTTAGCCAGA GAGCTGTGTA TCAACGCACC AGCTATCGAT
    CCATGTCTTT ATATCCCACG CTTTGCACAA CTGCTGGAAT TTGGAGAGAA GAGCCATGAG
    GTTTCTATGA CGGCACTGCG TCTCCTGCAG AGGATGAAGA GAGACTGGAT GCACACGGGA
    CGAAGACCTT CAGGCCTCTG CGGAGCAGCT CTCCTGGTTG CGGCCCGTAT GCACGAGTTC
    CGTCGCACAA TTAAAGAAGT GATTAGTGTG GTGAAAGTCT GCGAGGCCAC GCTAAGGAAA
    AGGTTGTATG AATTTGAGGA CACGCCCACC AGTGAGCTGA CTATTGAAGA GTTCATGAAG
    ACTGATCTGG AGCAGGAGTG CGACCCGCCT TCATTCACAG CAGGACTCAG GAAGCAAAAG
    CTAAAACAGA TTGAACAGGA GCTTGCGAAG AAAGTTGATG ATATTGAAGG TGAAATATGT
    GGCTATCAGG ATGAGATTGA AGTTGAGTTG GAAAGCTGTA GGCCCAAAGC TAGGGGGATT
    TATGCATCCT GTTCCAGAGA AGATGATGTG GTTTCCTTGG CATCCTCCAG TCACCTTGAT
    GGAGAAGATG ATGAACTGAG GGCAGCCGCA TCTCATCTGT GTGGGGAGAA TGAAGAGCAG
    GAGCAAGATG GAGAGAGAGT TTCTCTAACC AAACGGCCCT CGTTATCATT ATTGCTTGGA
    GCGCTGCCCA CTTCGGCCAG TTTGGGCCTG TCCGAGTCCA TCACCAAAAT GGGAGAGGAG
    AAGGAGAACG ATGTGGAGGC AGAAGGCGGT GAGCTAGACC TGAGCGGTAT TGACGAAGAT
    GAGATCGATC GGTATATCCT GAATGACAAA GAAGTTAAGG TGAAGACTGA ACTGTGGATG
    ATTCAAAACG CAGACTACCT CAAAGAGCAA AAAGAAAAGG AAGAAAGAAT AGCAAAAGAA
    AAGGAGGAAG GTACATATAA AGAGCGGAAG CCTAGAAAGT CAACACGAAG GCGTGAGCCC
    ATCAATGCCA ACACTGCAGA CGAAGCCATT GAAAAGATGT TAGAACAGAA ACGCATCTCC
    ACCAAAATAA ACTATGATGT TCTGAAAGAC TTGAACAAAT CCAGCCCAAA AAGTCCCACT
    CGCCAGCCTG AAGAAGCTTC AGCTGGAGCC AAAAGAACCC CCGCGGCCCG CCAACGGAAA
    CCCCAACTGA CTTCACCCGA TATTGGCAAA ACTGTCAGCA GCTTTGGCAA GAGGTTACAG
    CCCCTGGTGC GTGCGCAGCC CTCTAAGAAG CTGGCCCTTG ATCAGATAGG CTCTTCTGTT
    CCAGTGGCGA CTCCTGCTGT CTCAGCTCAG ACTGCTGTTG TTGTAGAGAG CGGACCAGTG
    GCTTATGAAG AGCATGCAGA TGAGGAAGAA GAGGATGAGG AGGATGAGCA CTGTGTCAGT
    GCCATGCAGT TAATGGGCGG CACTAGTGAT TATGGCTATG AATTAGATGA TGATGATGGC
    TTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214188.1
    CDS250..2235
    Translation

    Target ORF information:

    RefSeq Version XM_017358699.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio BRF1, RNA polymerase III transcription initiation factor a (brf1a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358699.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGGGCGGT GCAAGACCTG CGGTGGGACA GACATCGATG TGGACCAGGC TCGGGGTGTT 
    GCGGTGTGCA TGGGCTGTGG CTCTGTGCTG GAGGACAACA TCATCGTCTC TGAGGTCACC
    TTTGTGGAGA GTGGCGGCGG CGGATCGTCA GCTGTTGGCC AGTTCGTCGC TGGCGATGCA
    TCTGGAAATG TGCCTTCACT GGGAGGAAAT TTCCAAACTA GTGTCGGGAG AGAGTCCAGA
    GCTGCCACTC TGCAGAATGC GAAGCGACAG ATAAATCACC TTGGTCATCA GCTTCAGATG
    AATCAGCACT GTCTGGACAC TGCCTTCAAC TTTTATAAGA TGGCTCTCTC TAAACATCTG
    ACCAAAGGAC GCAAATCAAC TCATGTCATC GCCGCCTGCC TCTACCTCGT CTGCCGAACC
    GAGGGAACAC CTCATATGCT GCTTGATTTG AGTGACCTCT TGCAGGTGAA CGTGTACGTT
    TTGGGAAAAA CATTCCTTGT TTTAGCCAGA GAGCTGTGTA TCAACGCACC AGCTATCGAT
    CCATGTCTTT ATATCCCACG CTTTGCACAA CTGCTGGAAT TTGGAGAGAA GAGCCATGAG
    GTTTCTATGA CGGCACTGCG TCTCCTGCAG AGGATGAAGA GAGACTGGAT GCACACGGGA
    CGAAGACCTT CAGGCCTCTG CGGAGCAGCT CTCCTGGTTG CGGCCCGTAT GCACGAGTTC
    CGTCGCACAA TTAAAGAAGT GATTAGTGTG GTGAAAGTCT GCGAGGCCAC GCTAAGGAAA
    AGGTTGTATG AATTTGAGGA CACGCCCACC AGTGAGCTGA CTATTGAAGA GTTCATGAAG
    ACTGATCTGG AGCAGGAGTG CGACCCGCCT TCATTCACAG CAGGACTCAG GAAGCAAAAG
    CTAAAACAGA TTGAACAGGA GCTTGCGAAG AAAGTTGATG ATATTGAAGG TGAAATATGT
    GGCTATCAGG ATGAGATTGA AGTTGAGTTG GAAAGCTGTA GGCCCAAAGC TAGGGGGATT
    TATGCATCCT GTTCCAGAGA AGATGATGTG GTTTCCTTGG CATCCTCCAG TCACCTTGAT
    GGAGAAGATG ATGAACTGAG GGCAGCCGCA TCTCATCTGT GTGGGGAGAA TGAAGAGCAG
    GAGCAAGATG GAGAGAGAGT TTCTCTAACC AAACGGCCCT CGTTATCATT ATTGCTTGGA
    GCGCTGCCCA CTTCGGCCAG TTTGGGCCTG TCCGAGTCCA TCACCAAAAT GGGAGAGGAG
    AAGGAGAACG ATGTGGAGGC AGAAGGCGGT GAGCTAGACC TGAGCGGTAT TGACGAAGAT
    GAGATCGATC GGTATATCCT GAATGACAAA GAAGTTAAGG TGAAGACTGA ACTGTGGATG
    ATTCAAAACG CAGACTACCT CAAAGAGCAA AAAGAAAAGG AAGAAAGAAT AGCAAAAGAA
    AAGGAGGAAG GTACATATAA AGAGCGGAAG CCTAGAAAGT CAACACGAAG GCGTGAGCCC
    ATCAATGCCA ACACTGCAGA CGAAGCCATT GAAAAGATGT TAGAACAGAA ACGCATCTCC
    ACCAAAATAA ACTATGATGT TCTGAAAGAC TTGAACAAAT CCAGCCCAAA AAGTCCCACT
    CGCCAGCCTG AAGAAGCTTC AGCTGGAGCC AAAAGAACCC CCGCGGCCCG CCAACGGAAA
    CCCCAACTGA CTTCACCCGA TATTGGCAAA ACTGTCAGCA GCTTTGGCAA GAGGTTACAG
    CCCCTGGTGC GTGCGCAGCC CTCTAAGAAG CTGGCCCTTG ATCAGATAGG CTCTTCTGTT
    CCAGTGGCGA CTCCTGCTGT CTCAGCTCAG ACTGCTGTTG TTGTAGAGAG CGGACCAGTG
    GCTTATGAAG AGCATGCAGA TGAGGAAGAA GAGGATGAGG AGGATGAGCA CTGTGTCAGT
    GCCATGCAGT TAATGGGCGG CACTAGTGAT TATGGCTATG AATTAGATGA TGATGATGGC
    TTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa54671
    Clone ID Related Accession (Same CDS sequence) XM_017358699.2 , XM_017358699.1
    Accession Version XM_017358699.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 1986bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product BRF1, RNA polymerase III transcription initiation factor a isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007124.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGGGCGGT GCAAGACCTG CGGTGGGACA GACATCGATG TGGACCAGGC TCGGGGTGTT 
    GCGGTGTGCA TGGGCTGTGG CTCTGTGCTG GAGGACAACA TCATCGTCTC TGAGGTCACC
    TTTGTGGAGA GTGGCGGCGG CGGATCGTCA GCTGTTGGCC AGTTCGTCGC TGGCGATGCA
    TCTGGAAATG TGCCTTCACT GGGAGGAAAT TTCCAAACTA GTGTCGGGAG AGAGTCCAGA
    GCTGCCACTC TGCAGAATGC GAAGCGACAG ATAAATCACC TTGGTCATCA GCTTCAGATG
    AATCAGCACT GTCTGGACAC TGCCTTCAAC TTTTATAAGA TGGCTCTCTC TAAACATCTG
    ACCAAAGGAC GCAAATCAAC TCATGTCATC GCCGCCTGCC TCTACCTCGT CTGCCGAACC
    GAGGGAACAC CTCATATGCT GCTTGATTTG AGTGACCTCT TGCAGGTGAA CGTGTACGTT
    TTGGGAAAAA CATTCCTTGT TTTAGCCAGA GAGCTGTGTA TCAACGCACC AGCTATCGAT
    CCATGTCTTT ATATCCCACG CTTTGCACAA CTGCTGGAAT TTGGAGAGAA GAGCCATGAG
    GTTTCTATGA CGGCACTGCG TCTCCTGCAG AGGATGAAGA GAGACTGGAT GCACACGGGA
    CGAAGACCTT CAGGCCTCTG CGGAGCAGCT CTCCTGGTTG CGGCCCGTAT GCACGAGTTC
    CGTCGCACAA TTAAAGAAGT GATTAGTGTG GTGAAAGTCT GCGAGGCCAC GCTAAGGAAA
    AGGTTGTATG AATTTGAGGA CACGCCCACC AGTGAGCTGA CTATTGAAGA GTTCATGAAG
    ACTGATCTGG AGCAGGAGTG CGACCCGCCT TCATTCACAG CAGGACTCAG GAAGCAAAAG
    CTAAAACAGA TTGAACAGGA GCTTGCGAAG AAAGTTGATG ATATTGAAGG TGAAATATGT
    GGCTATCAGG ATGAGATTGA AGTTGAGTTG GAAAGCTGTA GGCCCAAAGC TAGGGGGATT
    TATGCATCCT GTTCCAGAGA AGATGATGTG GTTTCCTTGG CATCCTCCAG TCACCTTGAT
    GGAGAAGATG ATGAACTGAG GGCAGCCGCA TCTCATCTGT GTGGGGAGAA TGAAGAGCAG
    GAGCAAGATG GAGAGAGAGT TTCTCTAACC AAACGGCCCT CGTTATCATT ATTGCTTGGA
    GCGCTGCCCA CTTCGGCCAG TTTGGGCCTG TCCGAGTCCA TCACCAAAAT GGGAGAGGAG
    AAGGAGAACG ATGTGGAGGC AGAAGGCGGT GAGCTAGACC TGAGCGGTAT TGACGAAGAT
    GAGATCGATC GGTATATCCT GAATGACAAA GAAGTTAAGG TGAAGACTGA ACTGTGGATG
    ATTCAAAACG CAGACTACCT CAAAGAGCAA AAAGAAAAGG AAGAAAGAAT AGCAAAAGAA
    AAGGAGGAAG GTACATATAA AGAGCGGAAG CCTAGAAAGT CAACACGAAG GCGTGAGCCC
    ATCAATGCCA ACACTGCAGA CGAAGCCATT GAAAAGATGT TAGAACAGAA ACGCATCTCC
    ACCAAAATAA ACTATGATGT TCTGAAAGAC TTGAACAAAT CCAGCCCAAA AAGTCCCACT
    CGCCAGCCTG AAGAAGCTTC AGCTGGAGCC AAAAGAACCC CCGCGGCCCG CCAACGGAAA
    CCCCAACTGA CTTCACCCGA TATTGGCAAA ACTGTCAGCA GCTTTGGCAA GAGGTTACAG
    CCCCTGGTGC GTGCGCAGCC CTCTAAGAAG CTGGCCCTTG ATCAGATAGG CTCTTCTGTT
    CCAGTGGCGA CTCCTGCTGT CTCAGCTCAG ACTGCTGTTG TTGTAGAGAG CGGACCAGTG
    GCTTATGAAG AGCATGCAGA TGAGGAAGAA GAGGATGAGG AGGATGAGCA CTGTGTCAGT
    GCCATGCAGT TAATGGGCGG CACTAGTGAT TATGGCTATG AATTAGATGA TGATGATGGC
    TTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017214188.1
    CDS199..2184
    Translation

    Target ORF information:

    RefSeq Version XM_017358699.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio BRF1, RNA polymerase III transcription initiation factor a (brf1a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017358699.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    ATGGGGCGGT GCAAGACCTG CGGTGGGACA GACATCGATG TGGACCAGGC TCGGGGTGTT 
    GCGGTGTGCA TGGGCTGTGG CTCTGTGCTG GAGGACAACA TCATCGTCTC TGAGGTCACC
    TTTGTGGAGA GTGGCGGCGG CGGATCGTCA GCTGTTGGCC AGTTCGTCGC TGGCGATGCA
    TCTGGAAATG TGCCTTCACT GGGAGGAAAT TTCCAAACTA GTGTCGGGAG AGAGTCCAGA
    GCTGCCACTC TGCAGAATGC GAAGCGACAG ATAAATCACC TTGGTCATCA GCTTCAGATG
    AATCAGCACT GTCTGGACAC TGCCTTCAAC TTTTATAAGA TGGCTCTCTC TAAACATCTG
    ACCAAAGGAC GCAAATCAAC TCATGTCATC GCCGCCTGCC TCTACCTCGT CTGCCGAACC
    GAGGGAACAC CTCATATGCT GCTTGATTTG AGTGACCTCT TGCAGGTGAA CGTGTACGTT
    TTGGGAAAAA CATTCCTTGT TTTAGCCAGA GAGCTGTGTA TCAACGCACC AGCTATCGAT
    CCATGTCTTT ATATCCCACG CTTTGCACAA CTGCTGGAAT TTGGAGAGAA GAGCCATGAG
    GTTTCTATGA CGGCACTGCG TCTCCTGCAG AGGATGAAGA GAGACTGGAT GCACACGGGA
    CGAAGACCTT CAGGCCTCTG CGGAGCAGCT CTCCTGGTTG CGGCCCGTAT GCACGAGTTC
    CGTCGCACAA TTAAAGAAGT GATTAGTGTG GTGAAAGTCT GCGAGGCCAC GCTAAGGAAA
    AGGTTGTATG AATTTGAGGA CACGCCCACC AGTGAGCTGA CTATTGAAGA GTTCATGAAG
    ACTGATCTGG AGCAGGAGTG CGACCCGCCT TCATTCACAG CAGGACTCAG GAAGCAAAAG
    CTAAAACAGA TTGAACAGGA GCTTGCGAAG AAAGTTGATG ATATTGAAGG TGAAATATGT
    GGCTATCAGG ATGAGATTGA AGTTGAGTTG GAAAGCTGTA GGCCCAAAGC TAGGGGGATT
    TATGCATCCT GTTCCAGAGA AGATGATGTG GTTTCCTTGG CATCCTCCAG TCACCTTGAT
    GGAGAAGATG ATGAACTGAG GGCAGCCGCA TCTCATCTGT GTGGGGAGAA TGAAGAGCAG
    GAGCAAGATG GAGAGAGAGT TTCTCTAACC AAACGGCCCT CGTTATCATT ATTGCTTGGA
    GCGCTGCCCA CTTCGGCCAG TTTGGGCCTG TCCGAGTCCA TCACCAAAAT GGGAGAGGAG
    AAGGAGAACG ATGTGGAGGC AGAAGGCGGT GAGCTAGACC TGAGCGGTAT TGACGAAGAT
    GAGATCGATC GGTATATCCT GAATGACAAA GAAGTTAAGG TGAAGACTGA ACTGTGGATG
    ATTCAAAACG CAGACTACCT CAAAGAGCAA AAAGAAAAGG AAGAAAGAAT AGCAAAAGAA
    AAGGAGGAAG GTACATATAA AGAGCGGAAG CCTAGAAAGT CAACACGAAG GCGTGAGCCC
    ATCAATGCCA ACACTGCAGA CGAAGCCATT GAAAAGATGT TAGAACAGAA ACGCATCTCC
    ACCAAAATAA ACTATGATGT TCTGAAAGAC TTGAACAAAT CCAGCCCAAA AAGTCCCACT
    CGCCAGCCTG AAGAAGCTTC AGCTGGAGCC AAAAGAACCC CCGCGGCCCG CCAACGGAAA
    CCCCAACTGA CTTCACCCGA TATTGGCAAA ACTGTCAGCA GCTTTGGCAA GAGGTTACAG
    CCCCTGGTGC GTGCGCAGCC CTCTAAGAAG CTGGCCCTTG ATCAGATAGG CTCTTCTGTT
    CCAGTGGCGA CTCCTGCTGT CTCAGCTCAG ACTGCTGTTG TTGTAGAGAG CGGACCAGTG
    GCTTATGAAG AGCATGCAGA TGAGGAAGAA GAGGATGAGG AGGATGAGCA CTGTGTCAGT
    GCCATGCAGT TAATGGGCGG CACTAGTGAT TATGGCTATG AATTAGATGA TGATGATGGC
    TTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.