nf1a cDNA ORF clone, Danio rerio(zebrafish)

The following nf1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nf1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa39871 XM_021466445.1
Latest version!
Danio rerio neurofibromin 1a (nf1a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa39872 XM_021466446.1
Latest version!
Danio rerio neurofibromin 1a (nf1a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa39873 XM_009291764.3
Latest version!
Danio rerio neurofibromin 1a (nf1a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa39874 XM_021466447.1
Latest version!
Danio rerio neurofibromin 1a (nf1a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa39875 XM_021466448.1
Latest version!
Danio rerio neurofibromin 1a (nf1a), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa39876 XM_021466449.1
Latest version!
Danio rerio neurofibromin 1a (nf1a), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa39877 XM_021466450.1
Latest version!
Danio rerio neurofibromin 1a (nf1a), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa39878 XM_009291769.3
Latest version!
Danio rerio neurofibromin 1a (nf1a), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa55268 XM_017351191.1
Latest version!
Danio rerio neurofibromin 1a (nf1a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa55269 XM_017351192.1
Latest version!
Danio rerio neurofibromin 1a (nf1a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa55270 XM_017351193.1
Latest version!
Danio rerio neurofibromin 1a (nf1a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa55271 XM_017351194.1
Latest version!
Danio rerio neurofibromin 1a (nf1a), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa55272 XM_017351195.1
Latest version!
Danio rerio neurofibromin 1a (nf1a), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa55273 XM_017351196.1
Latest version!
Danio rerio neurofibromin 1a (nf1a), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa55274 XM_017351197.1
Latest version!
Danio rerio neurofibromin 1a (nf1a), transcript variant X9, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
View Old Accession Versions >>
ODa39873 XM_009291764.2 Danio rerio neurofibromin 1a (nf1a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa39878 XM_009291769.2 Danio rerio neurofibromin 1a (nf1a), transcript variant X8, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa39871
    Clone ID Related Accession (Same CDS sequence) XM_021466445.1
    Accession Version XM_021466445.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8496bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product neurofibromin isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCGGCGC ACAAGCCCGT GGAATGGGTC CAGGCCATCA TTAACAGATT CGACGAACAG 
    CTCCCAATCA AAACAGGACA GCAGAGCACC CACACCAAAG TGAGTACAGA GCACAACAAA
    GAATGTTTGA TCAACATCTC CAAGTACAAA TTTGCCCTCG TCATCAATGG CCTGACCAAC
    ATACTCAAAA ATGTGAACAA CATGCGGATA TTTGGCGAAG CTGCTGAGAA GAACCTCTAC
    TTCTCCCAGC TGATCATACT GGACACACTG GAGAAGTGTC TAGCAGGGCA ACCTAAGGAT
    TGCCTGAGGC TGGATGAGGC TATGTTGGTA AAACAGTTGC TGCCTGAAAT CTGTCACTTC
    CTGCACTCGT ACCGTGAGGG CCAGCAACAC GCCACTCAGC TGCGCAGCTC CGCCTCTGCT
    GTGCTTTTCT CTCTCAGCTG CAACAACTTT AATGCCGTCT TCAGTCGTAT CTCCACCAGG
    TTGCAAGAGC TCACAGTTTG TACAGAGGAC ACAGTGGATG TCCATGACAT TGAGCTCATT
    CAGTATATCA ATGTAGATTG CTCCAAGTTA AAGAGACTCC TACAAGAAGC AGTACTTAAG
    TTCAAGTCTT TGAAAAAGCC GGCTCAGCTT GCAGTAATCA GCAGTTTGGA AAAGGCTTTC
    TGGAACTGGG TTGAACACTA CCCTGATGAT TTCACTATGC TCTATCAGAG ACCCCAGGCC
    GACATGGCCG ACTGTGCAGA GAGACTGTTC GACCTAGTGG ACAGTTTTGC GGAAAGCGCA
    AAGAGAAAAG CTGCTGTGTG GCCACTGCAG ATCATCCTCC TCATCCTCTG TCCAGAGATC
    ACGCAGAGCA TCACCAAAGA AGTAGGGGGA GAGGAGAAAG CTAACAAGAA GTCATTTGTA
    GACAGTTTAC GAAAGGCTCT TGGCCAACAC AGCGGCAGCA AACAATTGAC GGAAAGTGCA
    GCTGTTGCAT GCGTCAAACT CTGCAAAGCC TGCACATATA TCAACTGGGA GGATAATTCT
    GTCATCTTTC TCCTGGTTCA GTCCATCGTC ATGGACCTTA AGGGTCTCCT GTTTAACCCC
    ACCAAACCTT TCTCAAGAGG TACGGGCTGC CAGAGCGCCG ATGTAGATTT GATGATCGAC
    TGCTTTGTGT CATGTTATCG CATCAATCCT CGCAACAACC AGCATTTTAA GGTGTGTCTG
    GCCTCGACTT CTCCTCCTAC CTTCCATTTC GTCCTTGTCA ACTCCCTGCA CAAAATTATC
    ACAAATTCTC ACCTGGACTG GTGGCCGAAG ATTGACGCCA TCTACTGCTA CTCAGGAGAA
    CTACGCACCA TGTTTGCAGA CACACTGGGT CGAGTGATTC AGAGTCTCAG TGCACATCCG
    CCTCTGAGGA TGACATCAAG TTTGACATTC AAAGAGAAAA TGACAACAAG TTTGAAGTTC
    AAGGAAAAGT CCACAGATCT TGATTCGCGT TCTTTGCTGC TGGCACTGGT CAAACTTATC
    CACGCTGACC CCAAACTAAT GCTGCACAAT CCGGGAAAAC CTGGTCAGGA GATTCAGAAC
    AGCACAGCTG AGATCATCAA AGGTCTGGTT CAGCTGGTTC CTCTTACCCA CAGTGCTGAG
    CTTTCCCAAG AGGCCATGGA GGCATTGCTT GTTTTACATC AGCCAGAAAC CATTGAGCTA
    TGGAACCCGG ACGCTCCCAT TGAGACCTTC TGGGACATCA GCTCTCAGGT GTTATTTCTA
    ATTTGTAAGA AGCTGATTGG ACACCAGATG ATCAACAGTA CAGAGATACT CAAGTGGCTC
    AGGGAGATTC TCATATGCAG GAACAAGTTT CTATTAAAGA ACAAGGAGTG TGCTACACAG
    GGCAGTCTGA TCCCCATCAG CAGACAGGCT CAGACCAAGC TGGAGGTGTG TCTGTACACG
    TTCCTGTGGA GTCCTGATGC AGAGGCTGTT TTGGTGGCCA TGTCCTGTTT CAGGCATCTC
    TGTGAGGAGG CCGATATTCG CTGCAGTGTC GATGAGCTGC CTGTTCACAC AGTCCTACCC
    AATTACAACA CCTTCATTGA GCTTGCATCC GTCAGCAATA TGATGGCCAC AGGTCGCAAC
    ACACTACAAA AAAGAGTGAT GGCACTTTTA AGAAGAATAA AGCACCCTAC AGCTGGAAAT
    GTAGAGGCAT GGGAAGACAC ATATGCAAAG TGGGAGCAAG CTACAAAACA GATACTCAAC
    CACCCAAAAA ACAAAGTGGA AGATGGACAG GTAGTTAACA TAAGATTGGT CAATTATTTC
    ATCTTTTTTT CCATTCATGG TCATCTATTT TCTGTTCGTC CTCAGGAGTG GATCAACATG
    ACTGGTTTCT TGTGCGCTCT GGGAGGTGTT TGTCTCCAAC AGCGCAGCAC GCCGAGTCTG
    GGCACCTACA GTCCTCCAAT GGGCCCCCTG ACCGAACGCA AGGGGTCCAT GATTTCCATG
    AGCTCCTGCG AGGTAAACAG CGAAACATCC CTGAATCGCT TTCTGGAGCG TCTGCTGGCT
    CTGATGGTCT GCACACATGA GAAAGTGGGA GTGCAAATTC GCACCAACGT CAAAGACCTG
    GTGGGACTGG AGCTCAGTCC GGCTCTCTAT TCAATGCTCT TCACTAAGCT GCGCAACAGC
    ATCGGACGCT TCTTTGACAC CCAGGGGCCG GTTCCTATTA CAGATGCAAA CACTCTGTTT
    GTAGAGCAGA CCATTGCTAT CATGAAGAAC TTGCTGGACA GTCACACTGA AGGAAGCTCA
    GAGCACCTTG GTCAAGCCAG CATTGAGACC ATGATGCTCA ATCTAGTTAG GTATGTGCGT
    ATTTTAGGGA ACATTGTTCA TGCCATTCAG ATCAAAACCA AGTTGTGTCA GTTAGTGGAG
    GTGATGATGG AGCGCAGGGA TGACCTCTCC TTCTGCCAGG AGATGAAATT CAGGAATAAA
    ATGGTGGAGT ACCTCACAGA CTGGGTGATG GGAACCTCCA ATCAAGCTGC GGACGATGAT
    GTCAAATGCC TGACCCGCGA TCTGGACCAG GCCAGTATGG AGGCTGTCGT GTCTCTGTTG
    GCTGGTCTTC CTCTACAGCC TGAGGAGGGT GACGGTGTTG AGCTCATGGA GGCCAAATCA
    CAGCTCTTCC TGAAGTACTT CACACTGTTC ATGAACCTGT TGAATGACTG CAGCGAGGTT
    GAGGAAGATG GCCAGCAGGT CGCAGGGAGG AAACGTGGCA TGTCTAGACG ACTGGCATCT
    CTGCGGCACT GCACAGTATT GGCTATGTCT AACTTGCTCA ATGCTAATGT GGATAGCGGC
    CTCATGCACT CAATCGGTCT GGGATACCAT AAGGACCTGC AGACACGGGC TACATTTATG
    GAAGTGCTCA CTAAGATCCT TCAGCAAGGG ACGGAGTTCG ACACGCTTGC AGAGACTGTG
    CTTGCAGATC GCTTTGAGAG ACTGGTCGAG CTGGTCACCA TGATGGGAGA TCAGGGAGAG
    CTGCCCATAG CCATGGCTCT GGCCAATGTG GTCCCATGCT CTCAATGGGA TGAGCTGGCC
    AGAGTGTTAG TGACTCTCTT CGACTCTCGC CATCTGCTGT ATCAGCTGTT GTGGAATATG
    TTCTCCAAAG AGGTGGAGCT GGCAGACTCA ATGCAGACGC TCTTTAGAGG AAATAGCCTA
    GCCAGCAAGA TTATGACCTT CTGCTTCAAG GTGTACGGAG CTGCGTATCT ACAGAAGCTT
    CTGGAGCCGC TGTTGAGAGG AATCATCACT ACACCAGAGT GGCAGCAGAT CAGTTTTGAA
    GTGGACCCTA CTAGACTTGA ACAGGCAGAG AACCTGGAGG AGAACCAGAA GAACCTGCTT
    CAGATCACTG ACCGCTTCTT CCTGGCCATC ATCAACTCCT CGAGCGAGTT TCCTCCACAG
    CTGCGCAGTG TGTGCCACTG TCTGTATCAG GCTACTTGCC ACTCCCTTCT GAATAAAGCC
    ACTGTTAAAG AAAAAAAGGA AAACAAAAAA TCCGTGGTCA GTCAGCGGTT TCCTCAGAAC
    AGCATTGGTG CTGTAGGAAG TGCCATGTTC CTGCGCTTTA TTAACCCAGC TATCGTGTCC
    CCATATGAGG CTGGAATCCT AGACAAGAAA CCCCCACCTC GCATCGAGCG TGGCCTTAAA
    CTCATGTCTA AGATTCTGCA GAGCATTGCT AATCATGTTC TCTTCACAAA AGAGGAGCAT
    ATGAGGCCCT TTAATGATTT TGTCAAAAGC AACTTCGATG CAGCCAGAAG GTTCTTCTTG
    GATATCGCAT CTGATTCTCC ACCAAGTGAT TCTGTGAACC ATAGTCTGTC TTTCATCAGT
    GACGGTAATG TGCTCGCTCT GCACCGGCTA TTATGGAACA ACCAGGAAAG AATTGGCCAA
    TACCTGTCCA GCAACAGGGA CCACAAGGCA GTGGGCCGTA GACCATTTGA CAAGATGGCC
    ACTCTTCTGG CGTACCTGGG TCCTCCTGAA CATAAGCCAG TAGCAGACAC ACACTGGTCC
    AGCCTCAACC TCACCAGCTC AAAGTTTGAG GAGTTCATGA CAAGGCATCA AGTCCATGAG
    AAGGAGGAAT TTAAAGCATT AAAAACCCTC AACATATTTT ACCAAGCTGG CACATCTAAG
    AACGGCAACC CTGTGTTTTA TTACATCGCA CGCAGGTTCA AAACTGGACA AATCAATGGT
    GATCTTCTGA TCTACCACGT CCTGCTGACG CTCAAGCCCT ACTATGCCAA GCATTATGAG
    ATCGTAGTTG ACCTTACTCA TGTGGGCCCC AGTAACCGTT TCAAGACGGA CTTCTTATCC
    AAATGGTTTG TGGTCTTTCC AAACTTCGCC TATGAGAACG TGGCTGCTGT GTACATCTAC
    AACTGCAACA CCTGGGTACG CGAGTACACC AAATACCACG AGCGACTGCT GACTGGCCTC
    AAGGGGAGCA AGAAGCTGGT GTTTATCGAT TCGCCTGCAC GTCTAGCTGA ACACATCGAA
    CCCGAACAGC AGAAGCTGCC CGCAGCTACT TTAGCACTGG AAGAAGACCT CAAAGTGTTC
    CACAACGCCC TAAAGCTTGC ACACAGAGAC ACAAAAGTGT CAATTAAGGT GGGATCCACA
    GCTGTACAGG TGACCTCGGC CGAAAGAACG CGTGTCCTAG GACAGCCTGT GTTCCTAAAT
    GACATCTACT ACGCTTCAGA GATCGAGGAG ATATGCCTGG TGGACGAGAA CCAGTTCACG
    CTAACCATCG CTAACCAGGG CACCCCACTC ACTTTCATGC ACCAGGAGTG TGAAGCCATC
    GTTCAGTCCA TCATCCACAT CCGCACGCGC TGGGAACTTT CCCAACCAGA CTCCATTCCC
    CAACACACCA AAATCAGGCC TAAGGATGTT CCTGGCACCC TGCTCAACAT CGCTCTTCTC
    AACCTGGGAA GCTCCGACCC CAGTCTGAGG TCTGCGGCCT ACAATCTCCT TTGTGCCTTA
    ACCTGCACCT TCAACCTGAA GATTGAAGGC CAGCTTCTGG AAACGTCTGG CTTGTGCATC
    CCAGCCAACA ACACGCTTTT TATTGTGTCC ATTAGCAAAA CGCTAGCAGC TAATGAACCC
    CACCTCACTC TGGAGTTCCT GGAAGAGTGC ATCTCAGGAT TCAGTAAATC AAGTATAGAG
    CTGAAGCATC TGTGTCTAGA ATATATGACT CCTTGGCTCC GTAATTTGGT GCGCTTTTGC
    AAACACAATG ATGACGCCAA ACGGCAGCGT GTCACGGCCA TCCTGGACAA GCTTATTACT
    ATGACCATCA ACGAGAAGCA GATGTACCCC TCCATACAGG CCAAGATATG GGGCAGCTTG
    GGTCAGATCA CAGACCTGCT TGATGTGGTG TTGGACAGCT TTATAAAGAC CAGCGCTACT
    GGCGGTTTAG GATCCATCAA AGCTGAAGTG ATGGCTGACA CTGCTGTAGC TTTGGCGTCA
    GGAAATGTCA AACTGGTCTC CAGCAAGGTG ATTGGCCGAA TGTGTAAGAT CATCGACAAG
    ACGTGTCTGT CTCCCACTCC GACCCTTGAG CAGCACCTCA TGTGGGACGA CATCGCCATC
    CTGGCCCGGT ACATGCTCAT GCTGTCCTTC AACAACTCTC TGGACGTGGC CGCTCATCTG
    CCATACCTGT TCCACGTCGT CACCCTGCTG GTGGCCACGG GCCCTCTCTC GCTGCGTGCA
    TCAACACACG GCCTGGTCAT CAACATCATT CACTCGCTCT GCACATGCTC ACAACTCAAC
    TTCAGTGAGG AGACTAAACA GGTGCTGCGC CTGAGTCTGA CTGAGTTCTC CCTGCCCAAG
    TTCTACCTGC TGTTCGGCAT CAGTAAGGTC AAATCTGCGG CGGTCATCGC TTTCCGTTCC
    AGCTACAGAG ACCGCTCATT CTCTCCAGGC TCTTATGAGA GAGAGACATT TGCTCTGTCT
    TCCCTGGAAA CAGTCACGGA GGCTCTTTTG GAGATCATGG AGGCCTGCAT GAGGGACATT
    CCTGGATGCA AATGGCTAGA TCAGTGGACT GAATTGGCGC AAAAGTTTGC GTTCCAGTAT
    AACCCCTCTC TTCAGCCTCG GGCGCTGGTG GTGTTCGGCT GCATCAGTAA ACGAGTGACT
    CACGGACAGC TAAAGCAGAT CATTCGTATT CTCAGTAAAG CCAGTCCTCT ACTCAGCATT
    GATCGGGGGC TGGAGAGCTG CTTAAAGGGC CCAGATAATT ACAACAGCCA GGTTCTCATC
    GAAGCCACAG TCATCGCTCT CACCAAACTA CAGCCTCTCC TCGATCAGGA TTCTCCTATG
    CATAAAGCTC TGTTCTGGGT GGCCGTGGCC GTGCTTCAGT TGGATGAAGT GAATCTGTAC
    TCCGCCGGCA CTGCCTTACT GGAGCAGAAC CTGCACACAC TGGACAGCCT CCGAGTGTTT
    AATGATAAGA GTCCTGAAGA GGTTTTTATG GAGATCCGGA AACCTCTAGA GTGGCACTGT
    AAACAAATGG ACCATTTTGT GGGACTCAAC TTTGCCTCCA ACTTCAATTT TGCATTAGTG
    GGCCATTTAC TTAAAGGATA CAGACATCCC TCTTCAACAA CAGTTGCCAG GACAGTTCGT
    ATCCTGCATA CACTCCTGGG CCTGGTGGGA AAACACCGCA GCTGTGACAA GTTTGAGGTG
    AACACGCAGA GTGTGGCCTA TCTGGCAGCT TTGCTCACAG TGTCAGAGGA AGTTAGGAGC
    CGCTGCAGTT TAAAGCACAG GAAATCATTG CTGCTTTCAG AAGTTACAAT GGAGAACTTC
    CCCATGGACA CATACACCGT TTACGACACT GAACCCGGGT GCAGGCTACT GCGTGAAAAC
    CAGCCTTGGG CTTCTCCGAA AGTGTCAGAG CGCAGTCTTG CACCTCCGTA CTCCACAATA
    GGGCAGACCA GCCTTCGTCC ACGCAAATCC ATGAGTGTAG ACATGGGCCA GCCGTCTCAG
    GCCAATGCGA AGAAACTACT GGGTATTAGC GGCACTAGAA AGAGTTTCGA TCACTTAATA
    TCAGAATCCA AAGCCCCAAA AAGACCAGAC ATGGAGTCGG GCATCACCAC ACCTCCAAAG
    ATGAGGCGGG TTTCTGAAAA TGACTATGAG ATCGAAACTC AGCGAATAGC CAACTCTCAT
    TTACGCAAAG TGTCTGTGTC TGAATCCAAT GTCCTCCTGG ACGAGAACGT ACTTACGGAC
    CCCAAGATCC AGACGTTGCT CCTCACTGTA CTGGCAACAC TGGTGAAGTA CACTACAGAC
    GAGTTTCATC AGCGGATCTT GTATGAGTAT TTGGCTGAGG CCAGTGTGGT CTTCCCCAAG
    GTCTTTCCAG TTGTGCACAA CCTGCTGGAC TCCAAGTTAA GCACCGTCCT TTCCATGTGC
    CAGGACCCGA ACCTTCTGAA CCCCGTCCAT GGCATCATTC AGAACGTGGT CTATCATGAG
    GAGTCTCCCC CTCAGTACCA GCCGTCCTGC TTGCAGAGCT TTGGTTTCAA TGGTCTTTGG
    AGGTTTGCCG GGCCGTTCTC TAAGCAAACC CAAATTCCAG ACTACGCCGA GCTGGTGGTT
    AAATTTTTGG ATGCTTTGAT TGACACGTAT CTCCCGGGCA TTGAGGAGGA AGGAGGAGAA
    GAGTCACTGC GGACCCCGAC CTCGCCATAT CCACCAACCG TCCAGAGCCA GCTGAGCATC
    ACAGCCAATC TCAACCTGTC CAACTCCATG ACATCACTTG CCACATCACA GCACTCCCCA
    GAGGGCCCTA TAGTTTATAG ACGGTCCCCA ACTCTCTCCC ATCCACGAAC TACAGAGGAG
    GTGCCACCGA AAACAGCTGC CTGTCTGCCT TGCTCTAAGT CGGCTGTATT CCCACCTCAC
    CTCGTCCTGC CAGGCATCGA CAAGGAGAAC GTTGAGATTT CTCCCACCTC GAGTCTGTCC
    ACCAGTGGGC GAATGCGGCA CGGCTCAGCA AGCCAGGTGC AGAAGCAGCG CAGCGCAGGC
    AGCTTCAAAC GGCCAAGCAT CAAAAAGATC GTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322120.1
    CDS247..8742
    Translation

    Target ORF information:

    RefSeq Version XM_021466445.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio neurofibromin 1a (nf1a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466445.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCGGCGC ACAAGCCCGT GGAATGGGTC CAGGCCATCA TTAACAGATT CGACGAACAG 
    CTCCCAATCA AAACAGGACA GCAGAGCACC CACACCAAAG TGAGTACAGA GCACAACAAA
    GAATGTTTGA TCAACATCTC CAAGTACAAA TTTGCCCTCG TCATCAATGG CCTGACCAAC
    ATACTCAAAA ATGTGAACAA CATGCGGATA TTTGGCGAAG CTGCTGAGAA GAACCTCTAC
    TTCTCCCAGC TGATCATACT GGACACACTG GAGAAGTGTC TAGCAGGGCA ACCTAAGGAT
    TGCCTGAGGC TGGATGAGGC TATGTTGGTA AAACAGTTGC TGCCTGAAAT CTGTCACTTC
    CTGCACTCGT ACCGTGAGGG CCAGCAACAC GCCACTCAGC TGCGCAGCTC CGCCTCTGCT
    GTGCTTTTCT CTCTCAGCTG CAACAACTTT AATGCCGTCT TCAGTCGTAT CTCCACCAGG
    TTGCAAGAGC TCACAGTTTG TACAGAGGAC ACAGTGGATG TCCATGACAT TGAGCTCATT
    CAGTATATCA ATGTAGATTG CTCCAAGTTA AAGAGACTCC TACAAGAAGC AGTACTTAAG
    TTCAAGTCTT TGAAAAAGCC GGCTCAGCTT GCAGTAATCA GCAGTTTGGA AAAGGCTTTC
    TGGAACTGGG TTGAACACTA CCCTGATGAT TTCACTATGC TCTATCAGAG ACCCCAGGCC
    GACATGGCCG ACTGTGCAGA GAGACTGTTC GACCTAGTGG ACAGTTTTGC GGAAAGCGCA
    AAGAGAAAAG CTGCTGTGTG GCCACTGCAG ATCATCCTCC TCATCCTCTG TCCAGAGATC
    ACGCAGAGCA TCACCAAAGA AGTAGGGGGA GAGGAGAAAG CTAACAAGAA GTCATTTGTA
    GACAGTTTAC GAAAGGCTCT TGGCCAACAC AGCGGCAGCA AACAATTGAC GGAAAGTGCA
    GCTGTTGCAT GCGTCAAACT CTGCAAAGCC TGCACATATA TCAACTGGGA GGATAATTCT
    GTCATCTTTC TCCTGGTTCA GTCCATCGTC ATGGACCTTA AGGGTCTCCT GTTTAACCCC
    ACCAAACCTT TCTCAAGAGG TACGGGCTGC CAGAGCGCCG ATGTAGATTT GATGATCGAC
    TGCTTTGTGT CATGTTATCG CATCAATCCT CGCAACAACC AGCATTTTAA GGTGTGTCTG
    GCCTCGACTT CTCCTCCTAC CTTCCATTTC GTCCTTGTCA ACTCCCTGCA CAAAATTATC
    ACAAATTCTC ACCTGGACTG GTGGCCGAAG ATTGACGCCA TCTACTGCTA CTCAGGAGAA
    CTACGCACCA TGTTTGCAGA CACACTGGGT CGAGTGATTC AGAGTCTCAG TGCACATCCG
    CCTCTGAGGA TGACATCAAG TTTGACATTC AAAGAGAAAA TGACAACAAG TTTGAAGTTC
    AAGGAAAAGT CCACAGATCT TGATTCGCGT TCTTTGCTGC TGGCACTGGT CAAACTTATC
    CACGCTGACC CCAAACTAAT GCTGCACAAT CCGGGAAAAC CTGGTCAGGA GATTCAGAAC
    AGCACAGCTG AGATCATCAA AGGTCTGGTT CAGCTGGTTC CTCTTACCCA CAGTGCTGAG
    CTTTCCCAAG AGGCCATGGA GGCATTGCTT GTTTTACATC AGCCAGAAAC CATTGAGCTA
    TGGAACCCGG ACGCTCCCAT TGAGACCTTC TGGGACATCA GCTCTCAGGT GTTATTTCTA
    ATTTGTAAGA AGCTGATTGG ACACCAGATG ATCAACAGTA CAGAGATACT CAAGTGGCTC
    AGGGAGATTC TCATATGCAG GAACAAGTTT CTATTAAAGA ACAAGGAGTG TGCTACACAG
    GGCAGTCTGA TCCCCATCAG CAGACAGGCT CAGACCAAGC TGGAGGTGTG TCTGTACACG
    TTCCTGTGGA GTCCTGATGC AGAGGCTGTT TTGGTGGCCA TGTCCTGTTT CAGGCATCTC
    TGTGAGGAGG CCGATATTCG CTGCAGTGTC GATGAGCTGC CTGTTCACAC AGTCCTACCC
    AATTACAACA CCTTCATTGA GCTTGCATCC GTCAGCAATA TGATGGCCAC AGGTCGCAAC
    ACACTACAAA AAAGAGTGAT GGCACTTTTA AGAAGAATAA AGCACCCTAC AGCTGGAAAT
    GTAGAGGCAT GGGAAGACAC ATATGCAAAG TGGGAGCAAG CTACAAAACA GATACTCAAC
    CACCCAAAAA ACAAAGTGGA AGATGGACAG GTAGTTAACA TAAGATTGGT CAATTATTTC
    ATCTTTTTTT CCATTCATGG TCATCTATTT TCTGTTCGTC CTCAGGAGTG GATCAACATG
    ACTGGTTTCT TGTGCGCTCT GGGAGGTGTT TGTCTCCAAC AGCGCAGCAC GCCGAGTCTG
    GGCACCTACA GTCCTCCAAT GGGCCCCCTG ACCGAACGCA AGGGGTCCAT GATTTCCATG
    AGCTCCTGCG AGGTAAACAG CGAAACATCC CTGAATCGCT TTCTGGAGCG TCTGCTGGCT
    CTGATGGTCT GCACACATGA GAAAGTGGGA GTGCAAATTC GCACCAACGT CAAAGACCTG
    GTGGGACTGG AGCTCAGTCC GGCTCTCTAT TCAATGCTCT TCACTAAGCT GCGCAACAGC
    ATCGGACGCT TCTTTGACAC CCAGGGGCCG GTTCCTATTA CAGATGCAAA CACTCTGTTT
    GTAGAGCAGA CCATTGCTAT CATGAAGAAC TTGCTGGACA GTCACACTGA AGGAAGCTCA
    GAGCACCTTG GTCAAGCCAG CATTGAGACC ATGATGCTCA ATCTAGTTAG GTATGTGCGT
    ATTTTAGGGA ACATTGTTCA TGCCATTCAG ATCAAAACCA AGTTGTGTCA GTTAGTGGAG
    GTGATGATGG AGCGCAGGGA TGACCTCTCC TTCTGCCAGG AGATGAAATT CAGGAATAAA
    ATGGTGGAGT ACCTCACAGA CTGGGTGATG GGAACCTCCA ATCAAGCTGC GGACGATGAT
    GTCAAATGCC TGACCCGCGA TCTGGACCAG GCCAGTATGG AGGCTGTCGT GTCTCTGTTG
    GCTGGTCTTC CTCTACAGCC TGAGGAGGGT GACGGTGTTG AGCTCATGGA GGCCAAATCA
    CAGCTCTTCC TGAAGTACTT CACACTGTTC ATGAACCTGT TGAATGACTG CAGCGAGGTT
    GAGGAAGATG GCCAGCAGGT CGCAGGGAGG AAACGTGGCA TGTCTAGACG ACTGGCATCT
    CTGCGGCACT GCACAGTATT GGCTATGTCT AACTTGCTCA ATGCTAATGT GGATAGCGGC
    CTCATGCACT CAATCGGTCT GGGATACCAT AAGGACCTGC AGACACGGGC TACATTTATG
    GAAGTGCTCA CTAAGATCCT TCAGCAAGGG ACGGAGTTCG ACACGCTTGC AGAGACTGTG
    CTTGCAGATC GCTTTGAGAG ACTGGTCGAG CTGGTCACCA TGATGGGAGA TCAGGGAGAG
    CTGCCCATAG CCATGGCTCT GGCCAATGTG GTCCCATGCT CTCAATGGGA TGAGCTGGCC
    AGAGTGTTAG TGACTCTCTT CGACTCTCGC CATCTGCTGT ATCAGCTGTT GTGGAATATG
    TTCTCCAAAG AGGTGGAGCT GGCAGACTCA ATGCAGACGC TCTTTAGAGG AAATAGCCTA
    GCCAGCAAGA TTATGACCTT CTGCTTCAAG GTGTACGGAG CTGCGTATCT ACAGAAGCTT
    CTGGAGCCGC TGTTGAGAGG AATCATCACT ACACCAGAGT GGCAGCAGAT CAGTTTTGAA
    GTGGACCCTA CTAGACTTGA ACAGGCAGAG AACCTGGAGG AGAACCAGAA GAACCTGCTT
    CAGATCACTG ACCGCTTCTT CCTGGCCATC ATCAACTCCT CGAGCGAGTT TCCTCCACAG
    CTGCGCAGTG TGTGCCACTG TCTGTATCAG GCTACTTGCC ACTCCCTTCT GAATAAAGCC
    ACTGTTAAAG AAAAAAAGGA AAACAAAAAA TCCGTGGTCA GTCAGCGGTT TCCTCAGAAC
    AGCATTGGTG CTGTAGGAAG TGCCATGTTC CTGCGCTTTA TTAACCCAGC TATCGTGTCC
    CCATATGAGG CTGGAATCCT AGACAAGAAA CCCCCACCTC GCATCGAGCG TGGCCTTAAA
    CTCATGTCTA AGATTCTGCA GAGCATTGCT AATCATGTTC TCTTCACAAA AGAGGAGCAT
    ATGAGGCCCT TTAATGATTT TGTCAAAAGC AACTTCGATG CAGCCAGAAG GTTCTTCTTG
    GATATCGCAT CTGATTCTCC ACCAAGTGAT TCTGTGAACC ATAGTCTGTC TTTCATCAGT
    GACGGTAATG TGCTCGCTCT GCACCGGCTA TTATGGAACA ACCAGGAAAG AATTGGCCAA
    TACCTGTCCA GCAACAGGGA CCACAAGGCA GTGGGCCGTA GACCATTTGA CAAGATGGCC
    ACTCTTCTGG CGTACCTGGG TCCTCCTGAA CATAAGCCAG TAGCAGACAC ACACTGGTCC
    AGCCTCAACC TCACCAGCTC AAAGTTTGAG GAGTTCATGA CAAGGCATCA AGTCCATGAG
    AAGGAGGAAT TTAAAGCATT AAAAACCCTC AACATATTTT ACCAAGCTGG CACATCTAAG
    AACGGCAACC CTGTGTTTTA TTACATCGCA CGCAGGTTCA AAACTGGACA AATCAATGGT
    GATCTTCTGA TCTACCACGT CCTGCTGACG CTCAAGCCCT ACTATGCCAA GCATTATGAG
    ATCGTAGTTG ACCTTACTCA TGTGGGCCCC AGTAACCGTT TCAAGACGGA CTTCTTATCC
    AAATGGTTTG TGGTCTTTCC AAACTTCGCC TATGAGAACG TGGCTGCTGT GTACATCTAC
    AACTGCAACA CCTGGGTACG CGAGTACACC AAATACCACG AGCGACTGCT GACTGGCCTC
    AAGGGGAGCA AGAAGCTGGT GTTTATCGAT TCGCCTGCAC GTCTAGCTGA ACACATCGAA
    CCCGAACAGC AGAAGCTGCC CGCAGCTACT TTAGCACTGG AAGAAGACCT CAAAGTGTTC
    CACAACGCCC TAAAGCTTGC ACACAGAGAC ACAAAAGTGT CAATTAAGGT GGGATCCACA
    GCTGTACAGG TGACCTCGGC CGAAAGAACG CGTGTCCTAG GACAGCCTGT GTTCCTAAAT
    GACATCTACT ACGCTTCAGA GATCGAGGAG ATATGCCTGG TGGACGAGAA CCAGTTCACG
    CTAACCATCG CTAACCAGGG CACCCCACTC ACTTTCATGC ACCAGGAGTG TGAAGCCATC
    GTTCAGTCCA TCATCCACAT CCGCACGCGC TGGGAACTTT CCCAACCAGA CTCCATTCCC
    CAACACACCA AAATCAGGCC TAAGGATGTT CCTGGCACCC TGCTCAACAT CGCTCTTCTC
    AACCTGGGAA GCTCCGACCC CAGTCTGAGG TCTGCGGCCT ACAATCTCCT TTGTGCCTTA
    ACCTGCACCT TCAACCTGAA GATTGAAGGC CAGCTTCTGG AAACGTCTGG CTTGTGCATC
    CCAGCCAACA ACACGCTTTT TATTGTGTCC ATTAGCAAAA CGCTAGCAGC TAATGAACCC
    CACCTCACTC TGGAGTTCCT GGAAGAGTGC ATCTCAGGAT TCAGTAAATC AAGTATAGAG
    CTGAAGCATC TGTGTCTAGA ATATATGACT CCTTGGCTCC GTAATTTGGT GCGCTTTTGC
    AAACACAATG ATGACGCCAA ACGGCAGCGT GTCACGGCCA TCCTGGACAA GCTTATTACT
    ATGACCATCA ACGAGAAGCA GATGTACCCC TCCATACAGG CCAAGATATG GGGCAGCTTG
    GGTCAGATCA CAGACCTGCT TGATGTGGTG TTGGACAGCT TTATAAAGAC CAGCGCTACT
    GGCGGTTTAG GATCCATCAA AGCTGAAGTG ATGGCTGACA CTGCTGTAGC TTTGGCGTCA
    GGAAATGTCA AACTGGTCTC CAGCAAGGTG ATTGGCCGAA TGTGTAAGAT CATCGACAAG
    ACGTGTCTGT CTCCCACTCC GACCCTTGAG CAGCACCTCA TGTGGGACGA CATCGCCATC
    CTGGCCCGGT ACATGCTCAT GCTGTCCTTC AACAACTCTC TGGACGTGGC CGCTCATCTG
    CCATACCTGT TCCACGTCGT CACCCTGCTG GTGGCCACGG GCCCTCTCTC GCTGCGTGCA
    TCAACACACG GCCTGGTCAT CAACATCATT CACTCGCTCT GCACATGCTC ACAACTCAAC
    TTCAGTGAGG AGACTAAACA GGTGCTGCGC CTGAGTCTGA CTGAGTTCTC CCTGCCCAAG
    TTCTACCTGC TGTTCGGCAT CAGTAAGGTC AAATCTGCGG CGGTCATCGC TTTCCGTTCC
    AGCTACAGAG ACCGCTCATT CTCTCCAGGC TCTTATGAGA GAGAGACATT TGCTCTGTCT
    TCCCTGGAAA CAGTCACGGA GGCTCTTTTG GAGATCATGG AGGCCTGCAT GAGGGACATT
    CCTGGATGCA AATGGCTAGA TCAGTGGACT GAATTGGCGC AAAAGTTTGC GTTCCAGTAT
    AACCCCTCTC TTCAGCCTCG GGCGCTGGTG GTGTTCGGCT GCATCAGTAA ACGAGTGACT
    CACGGACAGC TAAAGCAGAT CATTCGTATT CTCAGTAAAG CCAGTCCTCT ACTCAGCATT
    GATCGGGGGC TGGAGAGCTG CTTAAAGGGC CCAGATAATT ACAACAGCCA GGTTCTCATC
    GAAGCCACAG TCATCGCTCT CACCAAACTA CAGCCTCTCC TCGATCAGGA TTCTCCTATG
    CATAAAGCTC TGTTCTGGGT GGCCGTGGCC GTGCTTCAGT TGGATGAAGT GAATCTGTAC
    TCCGCCGGCA CTGCCTTACT GGAGCAGAAC CTGCACACAC TGGACAGCCT CCGAGTGTTT
    AATGATAAGA GTCCTGAAGA GGTTTTTATG GAGATCCGGA AACCTCTAGA GTGGCACTGT
    AAACAAATGG ACCATTTTGT GGGACTCAAC TTTGCCTCCA ACTTCAATTT TGCATTAGTG
    GGCCATTTAC TTAAAGGATA CAGACATCCC TCTTCAACAA CAGTTGCCAG GACAGTTCGT
    ATCCTGCATA CACTCCTGGG CCTGGTGGGA AAACACCGCA GCTGTGACAA GTTTGAGGTG
    AACACGCAGA GTGTGGCCTA TCTGGCAGCT TTGCTCACAG TGTCAGAGGA AGTTAGGAGC
    CGCTGCAGTT TAAAGCACAG GAAATCATTG CTGCTTTCAG AAGTTACAAT GGAGAACTTC
    CCCATGGACA CATACACCGT TTACGACACT GAACCCGGGT GCAGGCTACT GCGTGAAAAC
    CAGCCTTGGG CTTCTCCGAA AGTGTCAGAG CGCAGTCTTG CACCTCCGTA CTCCACAATA
    GGGCAGACCA GCCTTCGTCC ACGCAAATCC ATGAGTGTAG ACATGGGCCA GCCGTCTCAG
    GCCAATGCGA AGAAACTACT GGGTATTAGC GGCACTAGAA AGAGTTTCGA TCACTTAATA
    TCAGAATCCA AAGCCCCAAA AAGACCAGAC ATGGAGTCGG GCATCACCAC ACCTCCAAAG
    ATGAGGCGGG TTTCTGAAAA TGACTATGAG ATCGAAACTC AGCGAATAGC CAACTCTCAT
    TTACGCAAAG TGTCTGTGTC TGAATCCAAT GTCCTCCTGG ACGAGAACGT ACTTACGGAC
    CCCAAGATCC AGACGTTGCT CCTCACTGTA CTGGCAACAC TGGTGAAGTA CACTACAGAC
    GAGTTTCATC AGCGGATCTT GTATGAGTAT TTGGCTGAGG CCAGTGTGGT CTTCCCCAAG
    GTCTTTCCAG TTGTGCACAA CCTGCTGGAC TCCAAGTTAA GCACCGTCCT TTCCATGTGC
    CAGGACCCGA ACCTTCTGAA CCCCGTCCAT GGCATCATTC AGAACGTGGT CTATCATGAG
    GAGTCTCCCC CTCAGTACCA GCCGTCCTGC TTGCAGAGCT TTGGTTTCAA TGGTCTTTGG
    AGGTTTGCCG GGCCGTTCTC TAAGCAAACC CAAATTCCAG ACTACGCCGA GCTGGTGGTT
    AAATTTTTGG ATGCTTTGAT TGACACGTAT CTCCCGGGCA TTGAGGAGGA AGGAGGAGAA
    GAGTCACTGC GGACCCCGAC CTCGCCATAT CCACCAACCG TCCAGAGCCA GCTGAGCATC
    ACAGCCAATC TCAACCTGTC CAACTCCATG ACATCACTTG CCACATCACA GCACTCCCCA
    GAGGGCCCTA TAGTTTATAG ACGGTCCCCA ACTCTCTCCC ATCCACGAAC TACAGAGGAG
    GTGCCACCGA AAACAGCTGC CTGTCTGCCT TGCTCTAAGT CGGCTGTATT CCCACCTCAC
    CTCGTCCTGC CAGGCATCGA CAAGGAGAAC GTTGAGATTT CTCCCACCTC GAGTCTGTCC
    ACCAGTGGGC GAATGCGGCA CGGCTCAGCA AGCCAGGTGC AGAAGCAGCG CAGCGCAGGC
    AGCTTCAAAC GGCCAAGCAT CAAAAAGATC GTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa39872
    Clone ID Related Accession (Same CDS sequence) XM_021466446.1
    Accession Version XM_021466446.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8487bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product neurofibromin isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
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    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
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    1981
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    7861
    7921
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    8161
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    8401
    8461
    ATGGCGGCGC ACAAGCCCGT GGAATGGGTC CAGGCCATCA TTAACAGATT CGACGAACAG 
    CTCCCAATCA AAACAGGACA GCAGAGCACC CACACCAAAG TGAGTACAGA GCACAACAAA
    GAATGTTTGA TCAACATCTC CAAGTACAAA TTTGCCCTCG TCATCAATGG CCTGACCAAC
    ATACTCAAAA ATGTGAACAA CATGCGGATA TTTGGCGAAG CTGCTGAGAA GAACCTCTAC
    TTCTCCCAGC TGATCATACT GGACACACTG GAGAAGTGTC TAGCAGGGCA ACCTAAGGAT
    TGCCTGAGGC TGGATGAGGC TATGTTGGTA AAACAGTTGC TGCCTGAAAT CTGTCACTTC
    CTGCACTCGT ACCGTGAGGG CCAGCAACAC GCCACTCAGC TGCGCAGCTC CGCCTCTGCT
    GTGCTTTTCT CTCTCAGCTG CAACAACTTT AATGCCGTCT TCAGTCGTAT CTCCACCAGG
    TTGCAAGAGC TCACAGTTTG TACAGAGGAC ACAGTGGATG TCCATGACAT TGAGCTCATT
    CAGTATATCA ATGTAGATTG CTCCAAGTTA AAGAGACTCC TACAAGAAGC AGTACTTAAG
    TTCAAGTCTT TGAAAAAGCC GGCTCAGCTT GCAGTAATCA GCAGTTTGGA AAAGGCTTTC
    TGGAACTGGG TTGAACACTA CCCTGATGAT TTCACTATGC TCTATCAGAG ACCCCAGGCC
    GACATGGCCG ACTGTGCAGA GAGACTGTTC GACCTAGTGG ACAGTTTTGC GGAAAGCGCA
    AAGAGAAAAG CTGCTGTGTG GCCACTGCAG ATCATCCTCC TCATCCTCTG TCCAGAGATC
    ACGCAGAGCA TCACCAAAGA AGTAGGGGGA GAGGAGAAAG CTAACAAGAA GTCATTTGTA
    GACAGTTTAC GAAAGGCTCT TGGCCAACAC AGCGGCAGCA AACAATTGAC GGAAAGTGCA
    GCTGTTGCAT GCGTCAAACT CTGCAAAGCC TGCACATATA TCAACTGGGA GGATAATTCT
    GTCATCTTTC TCCTGGTTCA GTCCATCGTC ATGGACCTTA AGGGTCTCCT GTTTAACCCC
    ACCAAACCTT TCTCAAGAGG TACGGGCTGC CAGAGCGCCG ATGTAGATTT GATGATCGAC
    TGCTTTGTGT CATGTTATCG CATCAATCCT CGCAACAACC AGCATTTTAA GGTGTGTCTG
    GCCTCGACTT CTCCTCCTAC CTTCCATTTC GTCCTTGTCA ACTCCCTGCA CAAAATTATC
    ACAAATTCTC ACCTGGACTG GTGGCCGAAG ATTGACGCCA TCTACTGCTA CTCAGGAGAA
    CTACGCACCA TGTTTGCAGA CACACTGGGT CGAGTGATTC AGAGTCTCAG TGCACATCCG
    CCTCTGAGGA TGACATCAAG TTTGACATTC AAAGAGAAAA TGACAACAAG TTTGAAGTTC
    AAGGAAAAGT CCACAGATCT TGATTCGCGT TCTTTGCTGC TGGCACTGGT CAAACTTATC
    CACGCTGACC CCAAACTAAT GCTGCACAAT CCGGGAAAAC CTGGTCAGGA GATTCAGAAC
    AGCACAGCTG AGATCATCAA AGGTCTGGTT CAGCTGGTTC CTCTTACCCA CAGTGCTGAG
    CTTTCCCAAG AGGCCATGGA GGCATTGCTT GTTTTACATC AGCCAGAAAC CATTGAGCTA
    TGGAACCCGG ACGCTCCCAT TGAGACCTTC TGGGACATCA GCTCTCAGGT GTTATTTCTA
    ATTTGTAAGA AGCTGATTGG ACACCAGATG ATCAACAGTA CAGAGATACT CAAGTGGCTC
    AGGGAGATTC TCATATGCAG GAACAAGTTT CTATTAAAGA ACAAGGAGTG TGCTACACAG
    GGCAGTCTGA TCCCCATCAG CAGACAGGCT CAGACCAAGC TGGAGGTGTG TCTGTACACG
    TTCCTGTGGA GTCCTGATGC AGAGGCTGTT TTGGTGGCCA TGTCCTGTTT CAGGCATCTC
    TGTGAGGAGG CCGATATTCG CTGCAGTGTC GATGAGCTGC CTGTTCACAC AGTCCTACCC
    AATTACAACA CCTTCATTGA GCTTGCATCC GTCAGCAATA TGATGGCCAC AGGTCGCAAC
    ACACTACAAA AAAGAGTGAT GGCACTTTTA AGAAGAATAA AGCACCCTAC AGCTGGAAAT
    GTAGAGGCAT GGGAAGACAC ATATGCAAAG TGGGAGCAAG CTACAAAACA GATACTCAAC
    CACCCAAAAA ACAAAGTGGA AGATGGACAG GTAGTTAACA TAAGATTGGT CAATTATTTC
    ATCTTTTTTT CCATTCATGG TCATCTATTT TCTGTTCGTC CTCAGGAGTG GATCAACATG
    ACTGGTTTCT TGTGCGCTCT GGGAGGTGTT TGTCTCCAAC AGCGCAGCAC GCCGAGTCTG
    GGCACCTACA GTCCTCCAAT GGGCCCCCTG ACCGAACGCA AGGGGTCCAT GATTTCCATG
    AGCTCCTGCG AGGTAAACAG CGAAACATCC CTGAATCGCT TTCTGGAGCG TCTGCTGGCT
    CTGATGGTCT GCACACATGA GAAAGTGGGA GTGCAAATTC GCACCAACGT CAAAGACCTG
    GTGGGACTGG AGCTCAGTCC GGCTCTCTAT TCAATGCTCT TCACTAAGCT GCGCAACAGC
    ATCGGACGCT TCTTTGACAC CCAGGGGCCG GTTCCTATTA CAGATGCAAA CACTCTGTTT
    GTAGAGCAGA CCATTGCTAT CATGAAGAAC TTGCTGGACA GTCACACTGA AGGAAGCTCA
    GAGCACCTTG GTCAAGCCAG CATTGAGACC ATGATGCTCA ATCTAGTTAG GTATGTGCGT
    ATTTTAGGGA ACATTGTTCA TGCCATTCAG ATCAAAACCA AGTTGTGTCA GTTAGTGGAG
    GTGATGATGG AGCGCAGGGA TGACCTCTCC TTCTGCCAGG AGATGAAATT CAGGAATAAA
    ATGGTGGAGT ACCTCACAGA CTGGGTGATG GGAACCTCCA ATCAAGCTGC GGACGATGAT
    GTCAAATGCC TGACCCGCGA TCTGGACCAG GCCAGTATGG AGGCTGTCGT GTCTCTGTTG
    GCTGGTCTTC CTCTACAGCC TGAGGAGGGT GACGGTGTTG AGCTCATGGA GGCCAAATCA
    CAGCTCTTCC TGAAGTACTT CACACTGTTC ATGAACCTGT TGAATGACTG CAGCGAGGTT
    GAGGAAGATG GCCAGCAGGT CGCAGGGAGG AAACGTGGCA TGTCTAGACG ACTGGCATCT
    CTGCGGCACT GCACAGTATT GGCTATGTCT AACTTGCTCA ATGCTAATGT GGATAGCGGC
    CTCATGCACT CAATCGGTCT GGGATACCAT AAGGACCTGC AGACACGGGC TACATTTATG
    GAAGTGCTCA CTAAGATCCT TCAGCAAGGG ACGGAGTTCG ACACGCTTGC AGAGACTGTG
    CTTGCAGATC GCTTTGAGAG ACTGGTCGAG CTGGTCACCA TGATGGGAGA TCAGGGAGAG
    CTGCCCATAG CCATGGCTCT GGCCAATGTG GTCCCATGCT CTCAATGGGA TGAGCTGGCC
    AGAGTGTTAG TGACTCTCTT CGACTCTCGC CATCTGCTGT ATCAGCTGTT GTGGAATATG
    TTCTCCAAAG AGGTGGAGCT GGCAGACTCA ATGCAGACGC TCTTTAGAGG AAATAGCCTA
    GCCAGCAAGA TTATGACCTT CTGCTTCAAG GTGTACGGAG CTGCGTATCT ACAGAAGCTT
    CTGGAGCCGC TGTTGAGAGG AATCATCACT ACACCAGAGT GGCAGCAGAT CAGTTTTGAA
    GTGGACCCTA CTAGACTTGA ACAGGCAGAG AACCTGGAGG AGAACCAGAA GAACCTGCTT
    CAGATCACTG ACCGCTTCTT CCTGGCCATC ATCAACTCCT CGAGCGAGTT TCCTCCACAG
    CTGCGCAGTG TGTGCCACTG TCTGTATCAG GCTACTTGCC ACTCCCTTCT GAATAAAGCC
    ACTGTTAAAG AAAAAAAGGA AAACAAAAAA TCCGTGGTCA GTCAGCGGTT TCCTCAGAAC
    AGCATTGGTG CTGTAGGAAG TGCCATGTTC CTGCGCTTTA TTAACCCAGC TATCGTGTCC
    CCATATGAGG CTGGAATCCT AGACAAGAAA CCCCCACCTC GCATCGAGCG TGGCCTTAAA
    CTCATGTCTA AGATTCTGCA GAGCATTGCT AATCATGTTC TCTTCACAAA AGAGGAGCAT
    ATGAGGCCCT TTAATGATTT TGTCAAAAGC AACTTCGATG CAGCCAGAAG GTTCTTCTTG
    GATATCGCAT CTGATTCTCC ACCAAGTGAT TCTGTGAACC ATAGTCTGTC TTTCATCAGT
    GACGGTAATG TGCTCGCTCT GCACCGGCTA TTATGGAACA ACCAGGAAAG AATTGGCCAA
    TACCTGTCCA GCAACAGGGA CCACAAGGCA GTGGGCCGTA GACCATTTGA CAAGATGGCC
    ACTCTTCTGG CGTACCTGGG TCCTCCTGAA CATAAGCCAG TAGCAGACAC ACACTGGTCC
    AGCCTCAACC TCACCAGCTC AAAGTTTGAG GAGTTCATGA CAAGGCATCA AGTCCATGAG
    AAGGAGGAAT TTAAAGCATT AAAAACCCTC AACATATTTT ACCAAGCTGG CACATCTAAG
    AACGGCAACC CTGTGTTTTA TTACATCGCA CGCAGGTTCA AAACTGGACA AATCAATGGT
    GATCTTCTGA TCTACCACGT CCTGCTGACG CTCAAGCCCT ACTATGCCAA GCATTATGAG
    ATCGTAGTTG ACCTTACTCA TGTGGGCCCC AGTAACCGTT TCAAGACGGA CTTCTTATCC
    AAATGGTTTG TGGTCTTTCC AAACTTCGCC TATGAGAACG TGGCTGCTGT GTACATCTAC
    AACTGCAACA CCTGGGTACG CGAGTACACC AAATACCACG AGCGACTGCT GACTGGCCTC
    AAGGGGAGCA AGAAGCTGGT GTTTATCGAT TCGCCTGCAC GTCTAGCTGA ACACATCGAA
    CCCGAACAGC AGAAGCTGCC CGCAGCTACT TTAGCACTGG AAGAAGACCT CAAAGTGTTC
    CACAACGCCC TAAAGCTTGC ACACAGAGAC ACAAAAGTGT CAATTAAGGT GGGATCCACA
    GCTGTACAGG TGACCTCGGC CGAAAGAACG CGTGTCCTAG GACAGCCTGT GTTCCTAAAT
    GACATCTACT ACGCTTCAGA GATCGAGGAG ATATGCCTGG TGGACGAGAA CCAGTTCACG
    CTAACCATCG CTAACCAGGG CACCCCACTC ACTTTCATGC ACCAGGAGTG TGAAGCCATC
    GTTCAGTCCA TCATCCACAT CCGCACGCGC TGGGAACTTT CCCAACCAGA CTCCATTCCC
    CAACACACCA AAATCAGGCC TAAGGATGTT CCTGGCACCC TGCTCAACAT CGCTCTTCTC
    AACCTGGGAA GCTCCGACCC CAGTCTGAGG TCTGCGGCCT ACAATCTCCT TTGTGCCTTA
    ACCTGCACCT TCAACCTGAA GATTGAAGGC CAGCTTCTGG AAACGTCTGG CTTGTGCATC
    CCAGCCAACA ACACGCTTTT TATTGTGTCC ATTAGCAAAA CGCTAGCAGC TAATGAACCC
    CACCTCACTC TGGAGTTCCT GGAAGAGTGC ATCTCAGGAT TCAGTAAATC AAGTATAGAG
    CTGAAGCATC TGTGTCTAGA ATATATGACT CCTTGGCTCC GTAATTTGGT GCGCTTTTGC
    AAACACAATG ATGACGCCAA ACGGCAGCGT GTCACGGCCA TCCTGGACAA GCTTATTACT
    ATGACCATCA ACGAGAAGCA GATGTACCCC TCCATACAGG CCAAGATATG GGGCAGCTTG
    GGTCAGATCA CAGACCTGCT TGATGTGGTG TTGGACAGCT TTATAAAGAC CAGCGCTACT
    GGCGGTTTAG GATCCATCAA AGCTGAAGTG ATGGCTGACA CTGCTGTAGC TTTGGCGTCA
    GGAAATGTCA AACTGGTCTC CAGCAAGGTG ATTGGCCGAA TGTGTAAGAT CATCGACAAG
    ACGTGTCTGT CTCCCACTCC GACCCTTGAG CAGCACCTCA TGTGGGACGA CATCGCCATC
    CTGGCCCGGT ACATGCTCAT GCTGTCCTTC AACAACTCTC TGGACGTGGC CGCTCATCTG
    CCATACCTGT TCCACGTCGT CACCCTGCTG GTGGCCACGG GCCCTCTCTC GCTGCGTGCA
    TCAACACACG GCCTGGTCAT CAACATCATT CACTCGCTCT GCACATGCTC ACAACTCAAC
    TTCAGTGAGG AGACTAAACA GGTGCTGCGC CTGAGTCTGA CTGAGTTCTC CCTGCCCAAG
    TTCTACCTGC TGTTCGGCAT CAGTAAGGTC AAATCTGCGG CGGTCATCGC TTTCCGTTCC
    AGCTACAGAG ACCGCTCATT CTCTCCAGGC TCTTATGAGA GAGAGACATT TGCTCTGTCT
    TCCCTGGAAA CAGTCACGGA GGCTCTTTTG GAGATCATGG AGGCCTGCAT GAGGGACATT
    CCTGGATGCA AATGGCTAGA TCAGTGGACT GAATTGGCGC AAAAGTTTGC GTTCCAGTAT
    AACCCCTCTC TTCAGCCTCG GGCGCTGGTG GTGTTCGGCT GCATCAGTAA ACGAGTGACT
    CACGGACAGC TAAAGCAGAT CATTCGTATT CTCAGTAAAG CCAGTCCTCT ACTCAGCATT
    GATCGGGGGC TGGAGAGCTG CTTAAAGGGC CCAGATAATT ACAACAGCCA GGTTCTCATC
    GAAGCCACAG TCATCGCTCT CACCAAACTA CAGCCTCTCC TCGATCAGGA TTCTCCTATG
    CATAAAGCTC TGTTCTGGGT GGCCGTGGCC GTGCTTCAGT TGGATGAAGT GAATCTGTAC
    TCCGCCGGCA CTGCCTTACT GGAGCAGAAC CTGCACACAC TGGACAGCCT CCGAGTGTTT
    AATGATAAGA GTCCTGAAGA GGTTTTTATG GAGATCCGGA AACCTCTAGA GTGGCACTGT
    AAACAAATGG ACCATTTTGT GGGACTCAAC TTTGCCTCCA ACTTCAATTT TGCATTAGTG
    GGCCATTTAC TTAAAGGATA CAGACATCCC TCTTCAACAA CAGTTGCCAG GACAGTTCGT
    ATCCTGCATA CACTCCTGGG CCTGGTGGGA AAACACCGCA GCTGTGACAA GTTTGAGGTG
    AACACGCAGA GTGTGGCCTA TCTGGCAGCT TTGCTCACAG TGTCAGAGGA AGTTAGGAGC
    CGCTGCAGTT TAAAGCACAG GAAATCATTG CTGCTTTCAG AAGTTACAAT GGAGAACTTC
    CCCATGGACA CATACACCGT TTACGACACT GAACCCGGGT GCAGGCTACT GCGTGAAAAC
    CAGCCTTGGG CTTCTCCGAA AGTGTCAGAG CGCAGTCTTG CACCTCCGTA CTCCACAATA
    GGGCAGACCA GCCTTCGTCC ACGCAAATCC ATGAGTGTAG ACATGGGCCA GCCGTCTCAG
    GCCAATGCGA AGAAACTACT GGGCACTAGA AAGAGTTTCG ATCACTTAAT ATCAGAATCC
    AAAGCCCCAA AAAGACCAGA CATGGAGTCG GGCATCACCA CACCTCCAAA GATGAGGCGG
    GTTTCTGAAA ATGACTATGA GATCGAAACT CAGCGAATAG CCAACTCTCA TTTACGCAAA
    GTGTCTGTGT CTGAATCCAA TGTCCTCCTG GACGAGAACG TACTTACGGA CCCCAAGATC
    CAGACGTTGC TCCTCACTGT ACTGGCAACA CTGGTGAAGT ACACTACAGA CGAGTTTCAT
    CAGCGGATCT TGTATGAGTA TTTGGCTGAG GCCAGTGTGG TCTTCCCCAA GGTCTTTCCA
    GTTGTGCACA ACCTGCTGGA CTCCAAGTTA AGCACCGTCC TTTCCATGTG CCAGGACCCG
    AACCTTCTGA ACCCCGTCCA TGGCATCATT CAGAACGTGG TCTATCATGA GGAGTCTCCC
    CCTCAGTACC AGCCGTCCTG CTTGCAGAGC TTTGGTTTCA ATGGTCTTTG GAGGTTTGCC
    GGGCCGTTCT CTAAGCAAAC CCAAATTCCA GACTACGCCG AGCTGGTGGT TAAATTTTTG
    GATGCTTTGA TTGACACGTA TCTCCCGGGC ATTGAGGAGG AAGGAGGAGA AGAGTCACTG
    CGGACCCCGA CCTCGCCATA TCCACCAACC GTCCAGAGCC AGCTGAGCAT CACAGCCAAT
    CTCAACCTGT CCAACTCCAT GACATCACTT GCCACATCAC AGCACTCCCC AGAGGGCCCT
    ATAGTTTATA GACGGTCCCC AACTCTCTCC CATCCACGAA CTACAGAGGA GGTGCCACCG
    AAAACAGCTG CCTGTCTGCC TTGCTCTAAG TCGGCTGTAT TCCCACCTCA CCTCGTCCTG
    CCAGGCATCG ACAAGGAGAA CGTTGAGATT TCTCCCACCT CGAGTCTGTC CACCAGTGGG
    CGAATGCGGC ACGGCTCAGC AAGCCAGGTG CAGAAGCAGC GCAGCGCAGG CAGCTTCAAA
    CGGCCAAGCA TCAAAAAGAT CGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322121.1
    CDS247..8733
    Translation

    Target ORF information:

    RefSeq Version XM_021466446.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio neurofibromin 1a (nf1a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466446.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCGGCGC ACAAGCCCGT GGAATGGGTC CAGGCCATCA TTAACAGATT CGACGAACAG 
    CTCCCAATCA AAACAGGACA GCAGAGCACC CACACCAAAG TGAGTACAGA GCACAACAAA
    GAATGTTTGA TCAACATCTC CAAGTACAAA TTTGCCCTCG TCATCAATGG CCTGACCAAC
    ATACTCAAAA ATGTGAACAA CATGCGGATA TTTGGCGAAG CTGCTGAGAA GAACCTCTAC
    TTCTCCCAGC TGATCATACT GGACACACTG GAGAAGTGTC TAGCAGGGCA ACCTAAGGAT
    TGCCTGAGGC TGGATGAGGC TATGTTGGTA AAACAGTTGC TGCCTGAAAT CTGTCACTTC
    CTGCACTCGT ACCGTGAGGG CCAGCAACAC GCCACTCAGC TGCGCAGCTC CGCCTCTGCT
    GTGCTTTTCT CTCTCAGCTG CAACAACTTT AATGCCGTCT TCAGTCGTAT CTCCACCAGG
    TTGCAAGAGC TCACAGTTTG TACAGAGGAC ACAGTGGATG TCCATGACAT TGAGCTCATT
    CAGTATATCA ATGTAGATTG CTCCAAGTTA AAGAGACTCC TACAAGAAGC AGTACTTAAG
    TTCAAGTCTT TGAAAAAGCC GGCTCAGCTT GCAGTAATCA GCAGTTTGGA AAAGGCTTTC
    TGGAACTGGG TTGAACACTA CCCTGATGAT TTCACTATGC TCTATCAGAG ACCCCAGGCC
    GACATGGCCG ACTGTGCAGA GAGACTGTTC GACCTAGTGG ACAGTTTTGC GGAAAGCGCA
    AAGAGAAAAG CTGCTGTGTG GCCACTGCAG ATCATCCTCC TCATCCTCTG TCCAGAGATC
    ACGCAGAGCA TCACCAAAGA AGTAGGGGGA GAGGAGAAAG CTAACAAGAA GTCATTTGTA
    GACAGTTTAC GAAAGGCTCT TGGCCAACAC AGCGGCAGCA AACAATTGAC GGAAAGTGCA
    GCTGTTGCAT GCGTCAAACT CTGCAAAGCC TGCACATATA TCAACTGGGA GGATAATTCT
    GTCATCTTTC TCCTGGTTCA GTCCATCGTC ATGGACCTTA AGGGTCTCCT GTTTAACCCC
    ACCAAACCTT TCTCAAGAGG TACGGGCTGC CAGAGCGCCG ATGTAGATTT GATGATCGAC
    TGCTTTGTGT CATGTTATCG CATCAATCCT CGCAACAACC AGCATTTTAA GGTGTGTCTG
    GCCTCGACTT CTCCTCCTAC CTTCCATTTC GTCCTTGTCA ACTCCCTGCA CAAAATTATC
    ACAAATTCTC ACCTGGACTG GTGGCCGAAG ATTGACGCCA TCTACTGCTA CTCAGGAGAA
    CTACGCACCA TGTTTGCAGA CACACTGGGT CGAGTGATTC AGAGTCTCAG TGCACATCCG
    CCTCTGAGGA TGACATCAAG TTTGACATTC AAAGAGAAAA TGACAACAAG TTTGAAGTTC
    AAGGAAAAGT CCACAGATCT TGATTCGCGT TCTTTGCTGC TGGCACTGGT CAAACTTATC
    CACGCTGACC CCAAACTAAT GCTGCACAAT CCGGGAAAAC CTGGTCAGGA GATTCAGAAC
    AGCACAGCTG AGATCATCAA AGGTCTGGTT CAGCTGGTTC CTCTTACCCA CAGTGCTGAG
    CTTTCCCAAG AGGCCATGGA GGCATTGCTT GTTTTACATC AGCCAGAAAC CATTGAGCTA
    TGGAACCCGG ACGCTCCCAT TGAGACCTTC TGGGACATCA GCTCTCAGGT GTTATTTCTA
    ATTTGTAAGA AGCTGATTGG ACACCAGATG ATCAACAGTA CAGAGATACT CAAGTGGCTC
    AGGGAGATTC TCATATGCAG GAACAAGTTT CTATTAAAGA ACAAGGAGTG TGCTACACAG
    GGCAGTCTGA TCCCCATCAG CAGACAGGCT CAGACCAAGC TGGAGGTGTG TCTGTACACG
    TTCCTGTGGA GTCCTGATGC AGAGGCTGTT TTGGTGGCCA TGTCCTGTTT CAGGCATCTC
    TGTGAGGAGG CCGATATTCG CTGCAGTGTC GATGAGCTGC CTGTTCACAC AGTCCTACCC
    AATTACAACA CCTTCATTGA GCTTGCATCC GTCAGCAATA TGATGGCCAC AGGTCGCAAC
    ACACTACAAA AAAGAGTGAT GGCACTTTTA AGAAGAATAA AGCACCCTAC AGCTGGAAAT
    GTAGAGGCAT GGGAAGACAC ATATGCAAAG TGGGAGCAAG CTACAAAACA GATACTCAAC
    CACCCAAAAA ACAAAGTGGA AGATGGACAG GTAGTTAACA TAAGATTGGT CAATTATTTC
    ATCTTTTTTT CCATTCATGG TCATCTATTT TCTGTTCGTC CTCAGGAGTG GATCAACATG
    ACTGGTTTCT TGTGCGCTCT GGGAGGTGTT TGTCTCCAAC AGCGCAGCAC GCCGAGTCTG
    GGCACCTACA GTCCTCCAAT GGGCCCCCTG ACCGAACGCA AGGGGTCCAT GATTTCCATG
    AGCTCCTGCG AGGTAAACAG CGAAACATCC CTGAATCGCT TTCTGGAGCG TCTGCTGGCT
    CTGATGGTCT GCACACATGA GAAAGTGGGA GTGCAAATTC GCACCAACGT CAAAGACCTG
    GTGGGACTGG AGCTCAGTCC GGCTCTCTAT TCAATGCTCT TCACTAAGCT GCGCAACAGC
    ATCGGACGCT TCTTTGACAC CCAGGGGCCG GTTCCTATTA CAGATGCAAA CACTCTGTTT
    GTAGAGCAGA CCATTGCTAT CATGAAGAAC TTGCTGGACA GTCACACTGA AGGAAGCTCA
    GAGCACCTTG GTCAAGCCAG CATTGAGACC ATGATGCTCA ATCTAGTTAG GTATGTGCGT
    ATTTTAGGGA ACATTGTTCA TGCCATTCAG ATCAAAACCA AGTTGTGTCA GTTAGTGGAG
    GTGATGATGG AGCGCAGGGA TGACCTCTCC TTCTGCCAGG AGATGAAATT CAGGAATAAA
    ATGGTGGAGT ACCTCACAGA CTGGGTGATG GGAACCTCCA ATCAAGCTGC GGACGATGAT
    GTCAAATGCC TGACCCGCGA TCTGGACCAG GCCAGTATGG AGGCTGTCGT GTCTCTGTTG
    GCTGGTCTTC CTCTACAGCC TGAGGAGGGT GACGGTGTTG AGCTCATGGA GGCCAAATCA
    CAGCTCTTCC TGAAGTACTT CACACTGTTC ATGAACCTGT TGAATGACTG CAGCGAGGTT
    GAGGAAGATG GCCAGCAGGT CGCAGGGAGG AAACGTGGCA TGTCTAGACG ACTGGCATCT
    CTGCGGCACT GCACAGTATT GGCTATGTCT AACTTGCTCA ATGCTAATGT GGATAGCGGC
    CTCATGCACT CAATCGGTCT GGGATACCAT AAGGACCTGC AGACACGGGC TACATTTATG
    GAAGTGCTCA CTAAGATCCT TCAGCAAGGG ACGGAGTTCG ACACGCTTGC AGAGACTGTG
    CTTGCAGATC GCTTTGAGAG ACTGGTCGAG CTGGTCACCA TGATGGGAGA TCAGGGAGAG
    CTGCCCATAG CCATGGCTCT GGCCAATGTG GTCCCATGCT CTCAATGGGA TGAGCTGGCC
    AGAGTGTTAG TGACTCTCTT CGACTCTCGC CATCTGCTGT ATCAGCTGTT GTGGAATATG
    TTCTCCAAAG AGGTGGAGCT GGCAGACTCA ATGCAGACGC TCTTTAGAGG AAATAGCCTA
    GCCAGCAAGA TTATGACCTT CTGCTTCAAG GTGTACGGAG CTGCGTATCT ACAGAAGCTT
    CTGGAGCCGC TGTTGAGAGG AATCATCACT ACACCAGAGT GGCAGCAGAT CAGTTTTGAA
    GTGGACCCTA CTAGACTTGA ACAGGCAGAG AACCTGGAGG AGAACCAGAA GAACCTGCTT
    CAGATCACTG ACCGCTTCTT CCTGGCCATC ATCAACTCCT CGAGCGAGTT TCCTCCACAG
    CTGCGCAGTG TGTGCCACTG TCTGTATCAG GCTACTTGCC ACTCCCTTCT GAATAAAGCC
    ACTGTTAAAG AAAAAAAGGA AAACAAAAAA TCCGTGGTCA GTCAGCGGTT TCCTCAGAAC
    AGCATTGGTG CTGTAGGAAG TGCCATGTTC CTGCGCTTTA TTAACCCAGC TATCGTGTCC
    CCATATGAGG CTGGAATCCT AGACAAGAAA CCCCCACCTC GCATCGAGCG TGGCCTTAAA
    CTCATGTCTA AGATTCTGCA GAGCATTGCT AATCATGTTC TCTTCACAAA AGAGGAGCAT
    ATGAGGCCCT TTAATGATTT TGTCAAAAGC AACTTCGATG CAGCCAGAAG GTTCTTCTTG
    GATATCGCAT CTGATTCTCC ACCAAGTGAT TCTGTGAACC ATAGTCTGTC TTTCATCAGT
    GACGGTAATG TGCTCGCTCT GCACCGGCTA TTATGGAACA ACCAGGAAAG AATTGGCCAA
    TACCTGTCCA GCAACAGGGA CCACAAGGCA GTGGGCCGTA GACCATTTGA CAAGATGGCC
    ACTCTTCTGG CGTACCTGGG TCCTCCTGAA CATAAGCCAG TAGCAGACAC ACACTGGTCC
    AGCCTCAACC TCACCAGCTC AAAGTTTGAG GAGTTCATGA CAAGGCATCA AGTCCATGAG
    AAGGAGGAAT TTAAAGCATT AAAAACCCTC AACATATTTT ACCAAGCTGG CACATCTAAG
    AACGGCAACC CTGTGTTTTA TTACATCGCA CGCAGGTTCA AAACTGGACA AATCAATGGT
    GATCTTCTGA TCTACCACGT CCTGCTGACG CTCAAGCCCT ACTATGCCAA GCATTATGAG
    ATCGTAGTTG ACCTTACTCA TGTGGGCCCC AGTAACCGTT TCAAGACGGA CTTCTTATCC
    AAATGGTTTG TGGTCTTTCC AAACTTCGCC TATGAGAACG TGGCTGCTGT GTACATCTAC
    AACTGCAACA CCTGGGTACG CGAGTACACC AAATACCACG AGCGACTGCT GACTGGCCTC
    AAGGGGAGCA AGAAGCTGGT GTTTATCGAT TCGCCTGCAC GTCTAGCTGA ACACATCGAA
    CCCGAACAGC AGAAGCTGCC CGCAGCTACT TTAGCACTGG AAGAAGACCT CAAAGTGTTC
    CACAACGCCC TAAAGCTTGC ACACAGAGAC ACAAAAGTGT CAATTAAGGT GGGATCCACA
    GCTGTACAGG TGACCTCGGC CGAAAGAACG CGTGTCCTAG GACAGCCTGT GTTCCTAAAT
    GACATCTACT ACGCTTCAGA GATCGAGGAG ATATGCCTGG TGGACGAGAA CCAGTTCACG
    CTAACCATCG CTAACCAGGG CACCCCACTC ACTTTCATGC ACCAGGAGTG TGAAGCCATC
    GTTCAGTCCA TCATCCACAT CCGCACGCGC TGGGAACTTT CCCAACCAGA CTCCATTCCC
    CAACACACCA AAATCAGGCC TAAGGATGTT CCTGGCACCC TGCTCAACAT CGCTCTTCTC
    AACCTGGGAA GCTCCGACCC CAGTCTGAGG TCTGCGGCCT ACAATCTCCT TTGTGCCTTA
    ACCTGCACCT TCAACCTGAA GATTGAAGGC CAGCTTCTGG AAACGTCTGG CTTGTGCATC
    CCAGCCAACA ACACGCTTTT TATTGTGTCC ATTAGCAAAA CGCTAGCAGC TAATGAACCC
    CACCTCACTC TGGAGTTCCT GGAAGAGTGC ATCTCAGGAT TCAGTAAATC AAGTATAGAG
    CTGAAGCATC TGTGTCTAGA ATATATGACT CCTTGGCTCC GTAATTTGGT GCGCTTTTGC
    AAACACAATG ATGACGCCAA ACGGCAGCGT GTCACGGCCA TCCTGGACAA GCTTATTACT
    ATGACCATCA ACGAGAAGCA GATGTACCCC TCCATACAGG CCAAGATATG GGGCAGCTTG
    GGTCAGATCA CAGACCTGCT TGATGTGGTG TTGGACAGCT TTATAAAGAC CAGCGCTACT
    GGCGGTTTAG GATCCATCAA AGCTGAAGTG ATGGCTGACA CTGCTGTAGC TTTGGCGTCA
    GGAAATGTCA AACTGGTCTC CAGCAAGGTG ATTGGCCGAA TGTGTAAGAT CATCGACAAG
    ACGTGTCTGT CTCCCACTCC GACCCTTGAG CAGCACCTCA TGTGGGACGA CATCGCCATC
    CTGGCCCGGT ACATGCTCAT GCTGTCCTTC AACAACTCTC TGGACGTGGC CGCTCATCTG
    CCATACCTGT TCCACGTCGT CACCCTGCTG GTGGCCACGG GCCCTCTCTC GCTGCGTGCA
    TCAACACACG GCCTGGTCAT CAACATCATT CACTCGCTCT GCACATGCTC ACAACTCAAC
    TTCAGTGAGG AGACTAAACA GGTGCTGCGC CTGAGTCTGA CTGAGTTCTC CCTGCCCAAG
    TTCTACCTGC TGTTCGGCAT CAGTAAGGTC AAATCTGCGG CGGTCATCGC TTTCCGTTCC
    AGCTACAGAG ACCGCTCATT CTCTCCAGGC TCTTATGAGA GAGAGACATT TGCTCTGTCT
    TCCCTGGAAA CAGTCACGGA GGCTCTTTTG GAGATCATGG AGGCCTGCAT GAGGGACATT
    CCTGGATGCA AATGGCTAGA TCAGTGGACT GAATTGGCGC AAAAGTTTGC GTTCCAGTAT
    AACCCCTCTC TTCAGCCTCG GGCGCTGGTG GTGTTCGGCT GCATCAGTAA ACGAGTGACT
    CACGGACAGC TAAAGCAGAT CATTCGTATT CTCAGTAAAG CCAGTCCTCT ACTCAGCATT
    GATCGGGGGC TGGAGAGCTG CTTAAAGGGC CCAGATAATT ACAACAGCCA GGTTCTCATC
    GAAGCCACAG TCATCGCTCT CACCAAACTA CAGCCTCTCC TCGATCAGGA TTCTCCTATG
    CATAAAGCTC TGTTCTGGGT GGCCGTGGCC GTGCTTCAGT TGGATGAAGT GAATCTGTAC
    TCCGCCGGCA CTGCCTTACT GGAGCAGAAC CTGCACACAC TGGACAGCCT CCGAGTGTTT
    AATGATAAGA GTCCTGAAGA GGTTTTTATG GAGATCCGGA AACCTCTAGA GTGGCACTGT
    AAACAAATGG ACCATTTTGT GGGACTCAAC TTTGCCTCCA ACTTCAATTT TGCATTAGTG
    GGCCATTTAC TTAAAGGATA CAGACATCCC TCTTCAACAA CAGTTGCCAG GACAGTTCGT
    ATCCTGCATA CACTCCTGGG CCTGGTGGGA AAACACCGCA GCTGTGACAA GTTTGAGGTG
    AACACGCAGA GTGTGGCCTA TCTGGCAGCT TTGCTCACAG TGTCAGAGGA AGTTAGGAGC
    CGCTGCAGTT TAAAGCACAG GAAATCATTG CTGCTTTCAG AAGTTACAAT GGAGAACTTC
    CCCATGGACA CATACACCGT TTACGACACT GAACCCGGGT GCAGGCTACT GCGTGAAAAC
    CAGCCTTGGG CTTCTCCGAA AGTGTCAGAG CGCAGTCTTG CACCTCCGTA CTCCACAATA
    GGGCAGACCA GCCTTCGTCC ACGCAAATCC ATGAGTGTAG ACATGGGCCA GCCGTCTCAG
    GCCAATGCGA AGAAACTACT GGGCACTAGA AAGAGTTTCG ATCACTTAAT ATCAGAATCC
    AAAGCCCCAA AAAGACCAGA CATGGAGTCG GGCATCACCA CACCTCCAAA GATGAGGCGG
    GTTTCTGAAA ATGACTATGA GATCGAAACT CAGCGAATAG CCAACTCTCA TTTACGCAAA
    GTGTCTGTGT CTGAATCCAA TGTCCTCCTG GACGAGAACG TACTTACGGA CCCCAAGATC
    CAGACGTTGC TCCTCACTGT ACTGGCAACA CTGGTGAAGT ACACTACAGA CGAGTTTCAT
    CAGCGGATCT TGTATGAGTA TTTGGCTGAG GCCAGTGTGG TCTTCCCCAA GGTCTTTCCA
    GTTGTGCACA ACCTGCTGGA CTCCAAGTTA AGCACCGTCC TTTCCATGTG CCAGGACCCG
    AACCTTCTGA ACCCCGTCCA TGGCATCATT CAGAACGTGG TCTATCATGA GGAGTCTCCC
    CCTCAGTACC AGCCGTCCTG CTTGCAGAGC TTTGGTTTCA ATGGTCTTTG GAGGTTTGCC
    GGGCCGTTCT CTAAGCAAAC CCAAATTCCA GACTACGCCG AGCTGGTGGT TAAATTTTTG
    GATGCTTTGA TTGACACGTA TCTCCCGGGC ATTGAGGAGG AAGGAGGAGA AGAGTCACTG
    CGGACCCCGA CCTCGCCATA TCCACCAACC GTCCAGAGCC AGCTGAGCAT CACAGCCAAT
    CTCAACCTGT CCAACTCCAT GACATCACTT GCCACATCAC AGCACTCCCC AGAGGGCCCT
    ATAGTTTATA GACGGTCCCC AACTCTCTCC CATCCACGAA CTACAGAGGA GGTGCCACCG
    AAAACAGCTG CCTGTCTGCC TTGCTCTAAG TCGGCTGTAT TCCCACCTCA CCTCGTCCTG
    CCAGGCATCG ACAAGGAGAA CGTTGAGATT TCTCCCACCT CGAGTCTGTC CACCAGTGGG
    CGAATGCGGC ACGGCTCAGC AAGCCAGGTG CAGAAGCAGC GCAGCGCAGG CAGCTTCAAA
    CGGCCAAGCA TCAAAAAGAT CGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa39873
    Clone ID Related Accession (Same CDS sequence) XM_009291764.2 , XM_009291764.3
    Accession Version XM_009291764.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8433bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product neurofibromin isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291764.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGGCGGCGC ACAAGCCCGT GGAATGGGTC CAGGCCATCA TTAACAGATT CGACGAACAG 
    CTCCCAATCA AAACAGGACA GCAGAGCACC CACACCAAAG TGAGTACAGA GCACAACAAA
    GAATGTTTGA TCAACATCTC CAAGTACAAA TTTGCCCTCG TCATCAATGG CCTGACCAAC
    ATACTCAAAA ATGTGAACAA CATGCGGATA TTTGGCGAAG CTGCTGAGAA GAACCTCTAC
    TTCTCCCAGC TGATCATACT GGACACACTG GAGAAGTGTC TAGCAGGGCA ACCTAAGGAT
    TGCCTGAGGC TGGATGAGGC TATGTTGGTA AAACAGTTGC TGCCTGAAAT CTGTCACTTC
    CTGCACTCGT ACCGTGAGGG CCAGCAACAC GCCACTCAGC TGCGCAGCTC CGCCTCTGCT
    GTGCTTTTCT CTCTCAGCTG CAACAACTTT AATGCCGTCT TCAGTCGTAT CTCCACCAGG
    TTGCAAGAGC TCACAGTTTG TACAGAGGAC ACAGTGGATG TCCATGACAT TGAGCTCATT
    CAGTATATCA ATGTAGATTG CTCCAAGTTA AAGAGACTCC TACAAGAAGC AGTACTTAAG
    TTCAAGTCTT TGAAAAAGCC GGCTCAGCTT GCAGTAATCA GCAGTTTGGA AAAGGCTTTC
    TGGAACTGGG TTGAACACTA CCCTGATGAT TTCACTATGC TCTATCAGAG ACCCCAGGCC
    GACATGGCCG ACTGTGCAGA GAGACTGTTC GACCTAGTGG ACAGTTTTGC GGAAAGCGCA
    AAGAGAAAAG CTGCTGTGTG GCCACTGCAG ATCATCCTCC TCATCCTCTG TCCAGAGATC
    ACGCAGAGCA TCACCAAAGA AGTAGGGGGA GAGGAGAAAG CTAACAAGAA GTCATTTGTA
    GACAGTTTAC GAAAGGCTCT TGGCCAACAC AGCGGCAGCA AACAATTGAC GGAAAGTGCA
    GCTGTTGCAT GCGTCAAACT CTGCAAAGCC TGCACATATA TCAACTGGGA GGATAATTCT
    GTCATCTTTC TCCTGGTTCA GTCCATCGTC ATGGACCTTA AGGGTCTCCT GTTTAACCCC
    ACCAAACCTT TCTCAAGAGG TACGGGCTGC CAGAGCGCCG ATGTAGATTT GATGATCGAC
    TGCTTTGTGT CATGTTATCG CATCAATCCT CGCAACAACC AGCATTTTAA GGTGTGTCTG
    GCCTCGACTT CTCCTCCTAC CTTCCATTTC GTCCTTGTCA ACTCCCTGCA CAAAATTATC
    ACAAATTCTC ACCTGGACTG GTGGCCGAAG ATTGACGCCA TCTACTGCTA CTCAGGAGAA
    CTACGCACCA TGTTTGCAGA CACACTGGGT CGAGTGATTC AGAGTCTCAG TGCACATCCG
    CCTCTGAGGA TGACATCAAG TTTGACATTC AAAGAGAAAA TGACAACAAG TTTGAAGTTC
    AAGGAAAAGT CCACAGATCT TGATTCGCGT TCTTTGCTGC TGGCACTGGT CAAACTTATC
    CACGCTGACC CCAAACTAAT GCTGCACAAT CCGGGAAAAC CTGGTCAGGA GATTCAGAAC
    AGCACAGCTG AGATCATCAA AGGTCTGGTT CAGCTGGTTC CTCTTACCCA CAGTGCTGAG
    CTTTCCCAAG AGGCCATGGA GGCATTGCTT GTTTTACATC AGCCAGAAAC CATTGAGCTA
    TGGAACCCGG ACGCTCCCAT TGAGACCTTC TGGGACATCA GCTCTCAGGT GTTATTTCTA
    ATTTGTAAGA AGCTGATTGG ACACCAGATG ATCAACAGTA CAGAGATACT CAAGTGGCTC
    AGGGAGATTC TCATATGCAG GAACAAGTTT CTATTAAAGA ACAAGGAGTG TGCTACACAG
    GGCAGTCTGA TCCCCATCAG CAGACAGGCT CAGACCAAGC TGGAGGTGTG TCTGTACACG
    TTCCTGTGGA GTCCTGATGC AGAGGCTGTT TTGGTGGCCA TGTCCTGTTT CAGGCATCTC
    TGTGAGGAGG CCGATATTCG CTGCAGTGTC GATGAGCTGC CTGTTCACAC AGTCCTACCC
    AATTACAACA CCTTCATTGA GCTTGCATCC GTCAGCAATA TGATGGCCAC AGGTCGCAAC
    ACACTACAAA AAAGAGTGAT GGCACTTTTA AGAAGAATAA AGCACCCTAC AGCTGGAAAT
    GTAGAGGCAT GGGAAGACAC ATATGCAAAG TGGGAGCAAG CTACAAAACA GATACTCAAC
    CACCCAAAAA ACAAAGTGGA AGATGGACAG GTAGTTAACA TAAGATTGGT CAATTATTTC
    ATCTTTTTTT CCATTCATGG TCATCTATTT TCTGTTCGTC CTCAGGAGTG GATCAACATG
    ACTGGTTTCT TGTGCGCTCT GGGAGGTGTT TGTCTCCAAC AGCGCAGCAC GCCGAGTCTG
    GGCACCTACA GTCCTCCAAT GGGCCCCCTG ACCGAACGCA AGGGGTCCAT GATTTCCATG
    AGCTCCTGCG AGGTAAACAG CGAAACATCC CTGAATCGCT TTCTGGAGCG TCTGCTGGCT
    CTGATGGTCT GCACACATGA GAAAGTGGGA GTGCAAATTC GCACCAACGT CAAAGACCTG
    GTGGGACTGG AGCTCAGTCC GGCTCTCTAT TCAATGCTCT TCACTAAGCT GCGCAACAGC
    ATCGGACGCT TCTTTGACAC CCAGGGGCCG GTTCCTATTA CAGATGCAAA CACTCTGTTT
    GTAGAGCAGA CCATTGCTAT CATGAAGAAC TTGCTGGACA GTCACACTGA AGGAAGCTCA
    GAGCACCTTG GTCAAGCCAG CATTGAGACC ATGATGCTCA ATCTAGTTAG GTATGTGCGT
    ATTTTAGGGA ACATTGTTCA TGCCATTCAG ATCAAAACCA AGTTGTGTCA GTTAGTGGAG
    GTGATGATGG AGCGCAGGGA TGACCTCTCC TTCTGCCAGG AGATGAAATT CAGGAATAAA
    ATGGTGGAGT ACCTCACAGA CTGGGTGATG GGAACCTCCA ATCAAGCTGC GGACGATGAT
    GTCAAATGCC TGACCCGCGA TCTGGACCAG GCCAGTATGG AGGCTGTCGT GTCTCTGTTG
    GCTGGTCTTC CTCTACAGCC TGAGGAGGGT GACGGTGTTG AGCTCATGGA GGCCAAATCA
    CAGCTCTTCC TGAAGTACTT CACACTGTTC ATGAACCTGT TGAATGACTG CAGCGAGGTT
    GAGGAAGATG GCCAGCAGGT CGCAGGGAGG AAACGTGGCA TGTCTAGACG ACTGGCATCT
    CTGCGGCACT GCACAGTATT GGCTATGTCT AACTTGCTCA ATGCTAATGT GGATAGCGGC
    CTCATGCACT CAATCGGTCT GGGATACCAT AAGGACCTGC AGACACGGGC TACATTTATG
    GAAGTGCTCA CTAAGATCCT TCAGCAAGGG ACGGAGTTCG ACACGCTTGC AGAGACTGTG
    CTTGCAGATC GCTTTGAGAG ACTGGTCGAG CTGGTCACCA TGATGGGAGA TCAGGGAGAG
    CTGCCCATAG CCATGGCTCT GGCCAATGTG GTCCCATGCT CTCAATGGGA TGAGCTGGCC
    AGAGTGTTAG TGACTCTCTT CGACTCTCGC CATCTGCTGT ATCAGCTGTT GTGGAATATG
    TTCTCCAAAG AGGTGGAGCT GGCAGACTCA ATGCAGACGC TCTTTAGAGG AAATAGCCTA
    GCCAGCAAGA TTATGACCTT CTGCTTCAAG GTGTACGGAG CTGCGTATCT ACAGAAGCTT
    CTGGAGCCGC TGTTGAGAGG AATCATCACT ACACCAGAGT GGCAGCAGAT CAGTTTTGAA
    GTGGACCCTA CTAGACTTGA ACAGGCAGAG AACCTGGAGG AGAACCAGAA GAACCTGCTT
    CAGATCACTG ACCGCTTCTT CCTGGCCATC ATCAACTCCT CGAGCGAGTT TCCTCCACAG
    CTGCGCAGTG TGTGCCACTG TCTGTATCAG GTGGTCAGTC AGCGGTTTCC TCAGAACAGC
    ATTGGTGCTG TAGGAAGTGC CATGTTCCTG CGCTTTATTA ACCCAGCTAT CGTGTCCCCA
    TATGAGGCTG GAATCCTAGA CAAGAAACCC CCACCTCGCA TCGAGCGTGG CCTTAAACTC
    ATGTCTAAGA TTCTGCAGAG CATTGCTAAT CATGTTCTCT TCACAAAAGA GGAGCATATG
    AGGCCCTTTA ATGATTTTGT CAAAAGCAAC TTCGATGCAG CCAGAAGGTT CTTCTTGGAT
    ATCGCATCTG ATTCTCCACC AAGTGATTCT GTGAACCATA GTCTGTCTTT CATCAGTGAC
    GGTAATGTGC TCGCTCTGCA CCGGCTATTA TGGAACAACC AGGAAAGAAT TGGCCAATAC
    CTGTCCAGCA ACAGGGACCA CAAGGCAGTG GGCCGTAGAC CATTTGACAA GATGGCCACT
    CTTCTGGCGT ACCTGGGTCC TCCTGAACAT AAGCCAGTAG CAGACACACA CTGGTCCAGC
    CTCAACCTCA CCAGCTCAAA GTTTGAGGAG TTCATGACAA GGCATCAAGT CCATGAGAAG
    GAGGAATTTA AAGCATTAAA AACCCTCAAC ATATTTTACC AAGCTGGCAC ATCTAAGAAC
    GGCAACCCTG TGTTTTATTA CATCGCACGC AGGTTCAAAA CTGGACAAAT CAATGGTGAT
    CTTCTGATCT ACCACGTCCT GCTGACGCTC AAGCCCTACT ATGCCAAGCA TTATGAGATC
    GTAGTTGACC TTACTCATGT GGGCCCCAGT AACCGTTTCA AGACGGACTT CTTATCCAAA
    TGGTTTGTGG TCTTTCCAAA CTTCGCCTAT GAGAACGTGG CTGCTGTGTA CATCTACAAC
    TGCAACACCT GGGTACGCGA GTACACCAAA TACCACGAGC GACTGCTGAC TGGCCTCAAG
    GGGAGCAAGA AGCTGGTGTT TATCGATTCG CCTGCACGTC TAGCTGAACA CATCGAACCC
    GAACAGCAGA AGCTGCCCGC AGCTACTTTA GCACTGGAAG AAGACCTCAA AGTGTTCCAC
    AACGCCCTAA AGCTTGCACA CAGAGACACA AAAGTGTCAA TTAAGGTGGG ATCCACAGCT
    GTACAGGTGA CCTCGGCCGA AAGAACGCGT GTCCTAGGAC AGCCTGTGTT CCTAAATGAC
    ATCTACTACG CTTCAGAGAT CGAGGAGATA TGCCTGGTGG ACGAGAACCA GTTCACGCTA
    ACCATCGCTA ACCAGGGCAC CCCACTCACT TTCATGCACC AGGAGTGTGA AGCCATCGTT
    CAGTCCATCA TCCACATCCG CACGCGCTGG GAACTTTCCC AACCAGACTC CATTCCCCAA
    CACACCAAAA TCAGGCCTAA GGATGTTCCT GGCACCCTGC TCAACATCGC TCTTCTCAAC
    CTGGGAAGCT CCGACCCCAG TCTGAGGTCT GCGGCCTACA ATCTCCTTTG TGCCTTAACC
    TGCACCTTCA ACCTGAAGAT TGAAGGCCAG CTTCTGGAAA CGTCTGGCTT GTGCATCCCA
    GCCAACAACA CGCTTTTTAT TGTGTCCATT AGCAAAACGC TAGCAGCTAA TGAACCCCAC
    CTCACTCTGG AGTTCCTGGA AGAGTGCATC TCAGGATTCA GTAAATCAAG TATAGAGCTG
    AAGCATCTGT GTCTAGAATA TATGACTCCT TGGCTCCGTA ATTTGGTGCG CTTTTGCAAA
    CACAATGATG ACGCCAAACG GCAGCGTGTC ACGGCCATCC TGGACAAGCT TATTACTATG
    ACCATCAACG AGAAGCAGAT GTACCCCTCC ATACAGGCCA AGATATGGGG CAGCTTGGGT
    CAGATCACAG ACCTGCTTGA TGTGGTGTTG GACAGCTTTA TAAAGACCAG CGCTACTGGC
    GGTTTAGGAT CCATCAAAGC TGAAGTGATG GCTGACACTG CTGTAGCTTT GGCGTCAGGA
    AATGTCAAAC TGGTCTCCAG CAAGGTGATT GGCCGAATGT GTAAGATCAT CGACAAGACG
    TGTCTGTCTC CCACTCCGAC CCTTGAGCAG CACCTCATGT GGGACGACAT CGCCATCCTG
    GCCCGGTACA TGCTCATGCT GTCCTTCAAC AACTCTCTGG ACGTGGCCGC TCATCTGCCA
    TACCTGTTCC ACGTCGTCAC CCTGCTGGTG GCCACGGGCC CTCTCTCGCT GCGTGCATCA
    ACACACGGCC TGGTCATCAA CATCATTCAC TCGCTCTGCA CATGCTCACA ACTCAACTTC
    AGTGAGGAGA CTAAACAGGT GCTGCGCCTG AGTCTGACTG AGTTCTCCCT GCCCAAGTTC
    TACCTGCTGT TCGGCATCAG TAAGGTCAAA TCTGCGGCGG TCATCGCTTT CCGTTCCAGC
    TACAGAGACC GCTCATTCTC TCCAGGCTCT TATGAGAGAG AGACATTTGC TCTGTCTTCC
    CTGGAAACAG TCACGGAGGC TCTTTTGGAG ATCATGGAGG CCTGCATGAG GGACATTCCT
    GGATGCAAAT GGCTAGATCA GTGGACTGAA TTGGCGCAAA AGTTTGCGTT CCAGTATAAC
    CCCTCTCTTC AGCCTCGGGC GCTGGTGGTG TTCGGCTGCA TCAGTAAACG AGTGACTCAC
    GGACAGCTAA AGCAGATCAT TCGTATTCTC AGTAAAGCCA GTCCTCTACT CAGCATTGAT
    CGGGGGCTGG AGAGCTGCTT AAAGGGCCCA GATAATTACA ACAGCCAGGT TCTCATCGAA
    GCCACAGTCA TCGCTCTCAC CAAACTACAG CCTCTCCTCG ATCAGGATTC TCCTATGCAT
    AAAGCTCTGT TCTGGGTGGC CGTGGCCGTG CTTCAGTTGG ATGAAGTGAA TCTGTACTCC
    GCCGGCACTG CCTTACTGGA GCAGAACCTG CACACACTGG ACAGCCTCCG AGTGTTTAAT
    GATAAGAGTC CTGAAGAGGT TTTTATGGAG ATCCGGAAAC CTCTAGAGTG GCACTGTAAA
    CAAATGGACC ATTTTGTGGG ACTCAACTTT GCCTCCAACT TCAATTTTGC ATTAGTGGGC
    CATTTACTTA AAGGATACAG ACATCCCTCT TCAACAACAG TTGCCAGGAC AGTTCGTATC
    CTGCATACAC TCCTGGGCCT GGTGGGAAAA CACCGCAGCT GTGACAAGTT TGAGGTGAAC
    ACGCAGAGTG TGGCCTATCT GGCAGCTTTG CTCACAGTGT CAGAGGAAGT TAGGAGCCGC
    TGCAGTTTAA AGCACAGGAA ATCATTGCTG CTTTCAGAAG TTACAATGGA GAACTTCCCC
    ATGGACACAT ACACCGTTTA CGACACTGAA CCCGGGTGCA GGCTACTGCG TGAAAACCAG
    CCTTGGGCTT CTCCGAAAGT GTCAGAGCGC AGTCTTGCAC CTCCGTACTC CACAATAGGG
    CAGACCAGCC TTCGTCCACG CAAATCCATG AGTGTAGACA TGGGCCAGCC GTCTCAGGCC
    AATGCGAAGA AACTACTGGG TATTAGCGGC ACTAGAAAGA GTTTCGATCA CTTAATATCA
    GAATCCAAAG CCCCAAAAAG ACCAGACATG GAGTCGGGCA TCACCACACC TCCAAAGATG
    AGGCGGGTTT CTGAAAATGA CTATGAGATC GAAACTCAGC GAATAGCCAA CTCTCATTTA
    CGCAAAGTGT CTGTGTCTGA ATCCAATGTC CTCCTGGACG AGAACGTACT TACGGACCCC
    AAGATCCAGA CGTTGCTCCT CACTGTACTG GCAACACTGG TGAAGTACAC TACAGACGAG
    TTTCATCAGC GGATCTTGTA TGAGTATTTG GCTGAGGCCA GTGTGGTCTT CCCCAAGGTC
    TTTCCAGTTG TGCACAACCT GCTGGACTCC AAGTTAAGCA CCGTCCTTTC CATGTGCCAG
    GACCCGAACC TTCTGAACCC CGTCCATGGC ATCATTCAGA ACGTGGTCTA TCATGAGGAG
    TCTCCCCCTC AGTACCAGCC GTCCTGCTTG CAGAGCTTTG GTTTCAATGG TCTTTGGAGG
    TTTGCCGGGC CGTTCTCTAA GCAAACCCAA ATTCCAGACT ACGCCGAGCT GGTGGTTAAA
    TTTTTGGATG CTTTGATTGA CACGTATCTC CCGGGCATTG AGGAGGAAGG AGGAGAAGAG
    TCACTGCGGA CCCCGACCTC GCCATATCCA CCAACCGTCC AGAGCCAGCT GAGCATCACA
    GCCAATCTCA ACCTGTCCAA CTCCATGACA TCACTTGCCA CATCACAGCA CTCCCCAGAG
    GGCCCTATAG TTTATAGACG GTCCCCAACT CTCTCCCATC CACGAACTAC AGAGGAGGTG
    CCACCGAAAA CAGCTGCCTG TCTGCCTTGC TCTAAGTCGG CTGTATTCCC ACCTCACCTC
    GTCCTGCCAG GCATCGACAA GGAGAACGTT GAGATTTCTC CCACCTCGAG TCTGTCCACC
    AGTGGGCGAA TGCGGCACGG CTCAGCAAGC CAGGTGCAGA AGCAGCGCAG CGCAGGCAGC
    TTCAAACGGC CAAGCATCAA AAAGATCGTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290039.1
    CDS253..8685
    Translation

    Target ORF information:

    RefSeq Version XM_009291764.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio neurofibromin 1a (nf1a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009291764.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGGCGGCGC ACAAGCCCGT GGAATGGGTC CAGGCCATCA TTAACAGATT CGACGAACAG 
    CTCCCAATCA AAACAGGACA GCAGAGCACC CACACCAAAG TGAGTACAGA GCACAACAAA
    GAATGTTTGA TCAACATCTC CAAGTACAAA TTTGCCCTCG TCATCAATGG CCTGACCAAC
    ATACTCAAAA ATGTGAACAA CATGCGGATA TTTGGCGAAG CTGCTGAGAA GAACCTCTAC
    TTCTCCCAGC TGATCATACT GGACACACTG GAGAAGTGTC TAGCAGGGCA ACCTAAGGAT
    TGCCTGAGGC TGGATGAGGC TATGTTGGTA AAACAGTTGC TGCCTGAAAT CTGTCACTTC
    CTGCACTCGT ACCGTGAGGG CCAGCAACAC GCCACTCAGC TGCGCAGCTC CGCCTCTGCT
    GTGCTTTTCT CTCTCAGCTG CAACAACTTT AATGCCGTCT TCAGTCGTAT CTCCACCAGG
    TTGCAAGAGC TCACAGTTTG TACAGAGGAC ACAGTGGATG TCCATGACAT TGAGCTCATT
    CAGTATATCA ATGTAGATTG CTCCAAGTTA AAGAGACTCC TACAAGAAGC AGTACTTAAG
    TTCAAGTCTT TGAAAAAGCC GGCTCAGCTT GCAGTAATCA GCAGTTTGGA AAAGGCTTTC
    TGGAACTGGG TTGAACACTA CCCTGATGAT TTCACTATGC TCTATCAGAG ACCCCAGGCC
    GACATGGCCG ACTGTGCAGA GAGACTGTTC GACCTAGTGG ACAGTTTTGC GGAAAGCGCA
    AAGAGAAAAG CTGCTGTGTG GCCACTGCAG ATCATCCTCC TCATCCTCTG TCCAGAGATC
    ACGCAGAGCA TCACCAAAGA AGTAGGGGGA GAGGAGAAAG CTAACAAGAA GTCATTTGTA
    GACAGTTTAC GAAAGGCTCT TGGCCAACAC AGCGGCAGCA AACAATTGAC GGAAAGTGCA
    GCTGTTGCAT GCGTCAAACT CTGCAAAGCC TGCACATATA TCAACTGGGA GGATAATTCT
    GTCATCTTTC TCCTGGTTCA GTCCATCGTC ATGGACCTTA AGGGTCTCCT GTTTAACCCC
    ACCAAACCTT TCTCAAGAGG TACGGGCTGC CAGAGCGCCG ATGTAGATTT GATGATCGAC
    TGCTTTGTGT CATGTTATCG CATCAATCCT CGCAACAACC AGCATTTTAA GGTGTGTCTG
    GCCTCGACTT CTCCTCCTAC CTTCCATTTC GTCCTTGTCA ACTCCCTGCA CAAAATTATC
    ACAAATTCTC ACCTGGACTG GTGGCCGAAG ATTGACGCCA TCTACTGCTA CTCAGGAGAA
    CTACGCACCA TGTTTGCAGA CACACTGGGT CGAGTGATTC AGAGTCTCAG TGCACATCCG
    CCTCTGAGGA TGACATCAAG TTTGACATTC AAAGAGAAAA TGACAACAAG TTTGAAGTTC
    AAGGAAAAGT CCACAGATCT TGATTCGCGT TCTTTGCTGC TGGCACTGGT CAAACTTATC
    CACGCTGACC CCAAACTAAT GCTGCACAAT CCGGGAAAAC CTGGTCAGGA GATTCAGAAC
    AGCACAGCTG AGATCATCAA AGGTCTGGTT CAGCTGGTTC CTCTTACCCA CAGTGCTGAG
    CTTTCCCAAG AGGCCATGGA GGCATTGCTT GTTTTACATC AGCCAGAAAC CATTGAGCTA
    TGGAACCCGG ACGCTCCCAT TGAGACCTTC TGGGACATCA GCTCTCAGGT GTTATTTCTA
    ATTTGTAAGA AGCTGATTGG ACACCAGATG ATCAACAGTA CAGAGATACT CAAGTGGCTC
    AGGGAGATTC TCATATGCAG GAACAAGTTT CTATTAAAGA ACAAGGAGTG TGCTACACAG
    GGCAGTCTGA TCCCCATCAG CAGACAGGCT CAGACCAAGC TGGAGGTGTG TCTGTACACG
    TTCCTGTGGA GTCCTGATGC AGAGGCTGTT TTGGTGGCCA TGTCCTGTTT CAGGCATCTC
    TGTGAGGAGG CCGATATTCG CTGCAGTGTC GATGAGCTGC CTGTTCACAC AGTCCTACCC
    AATTACAACA CCTTCATTGA GCTTGCATCC GTCAGCAATA TGATGGCCAC AGGTCGCAAC
    ACACTACAAA AAAGAGTGAT GGCACTTTTA AGAAGAATAA AGCACCCTAC AGCTGGAAAT
    GTAGAGGCAT GGGAAGACAC ATATGCAAAG TGGGAGCAAG CTACAAAACA GATACTCAAC
    CACCCAAAAA ACAAAGTGGA AGATGGACAG GTAGTTAACA TAAGATTGGT CAATTATTTC
    ATCTTTTTTT CCATTCATGG TCATCTATTT TCTGTTCGTC CTCAGGAGTG GATCAACATG
    ACTGGTTTCT TGTGCGCTCT GGGAGGTGTT TGTCTCCAAC AGCGCAGCAC GCCGAGTCTG
    GGCACCTACA GTCCTCCAAT GGGCCCCCTG ACCGAACGCA AGGGGTCCAT GATTTCCATG
    AGCTCCTGCG AGGTAAACAG CGAAACATCC CTGAATCGCT TTCTGGAGCG TCTGCTGGCT
    CTGATGGTCT GCACACATGA GAAAGTGGGA GTGCAAATTC GCACCAACGT CAAAGACCTG
    GTGGGACTGG AGCTCAGTCC GGCTCTCTAT TCAATGCTCT TCACTAAGCT GCGCAACAGC
    ATCGGACGCT TCTTTGACAC CCAGGGGCCG GTTCCTATTA CAGATGCAAA CACTCTGTTT
    GTAGAGCAGA CCATTGCTAT CATGAAGAAC TTGCTGGACA GTCACACTGA AGGAAGCTCA
    GAGCACCTTG GTCAAGCCAG CATTGAGACC ATGATGCTCA ATCTAGTTAG GTATGTGCGT
    ATTTTAGGGA ACATTGTTCA TGCCATTCAG ATCAAAACCA AGTTGTGTCA GTTAGTGGAG
    GTGATGATGG AGCGCAGGGA TGACCTCTCC TTCTGCCAGG AGATGAAATT CAGGAATAAA
    ATGGTGGAGT ACCTCACAGA CTGGGTGATG GGAACCTCCA ATCAAGCTGC GGACGATGAT
    GTCAAATGCC TGACCCGCGA TCTGGACCAG GCCAGTATGG AGGCTGTCGT GTCTCTGTTG
    GCTGGTCTTC CTCTACAGCC TGAGGAGGGT GACGGTGTTG AGCTCATGGA GGCCAAATCA
    CAGCTCTTCC TGAAGTACTT CACACTGTTC ATGAACCTGT TGAATGACTG CAGCGAGGTT
    GAGGAAGATG GCCAGCAGGT CGCAGGGAGG AAACGTGGCA TGTCTAGACG ACTGGCATCT
    CTGCGGCACT GCACAGTATT GGCTATGTCT AACTTGCTCA ATGCTAATGT GGATAGCGGC
    CTCATGCACT CAATCGGTCT GGGATACCAT AAGGACCTGC AGACACGGGC TACATTTATG
    GAAGTGCTCA CTAAGATCCT TCAGCAAGGG ACGGAGTTCG ACACGCTTGC AGAGACTGTG
    CTTGCAGATC GCTTTGAGAG ACTGGTCGAG CTGGTCACCA TGATGGGAGA TCAGGGAGAG
    CTGCCCATAG CCATGGCTCT GGCCAATGTG GTCCCATGCT CTCAATGGGA TGAGCTGGCC
    AGAGTGTTAG TGACTCTCTT CGACTCTCGC CATCTGCTGT ATCAGCTGTT GTGGAATATG
    TTCTCCAAAG AGGTGGAGCT GGCAGACTCA ATGCAGACGC TCTTTAGAGG AAATAGCCTA
    GCCAGCAAGA TTATGACCTT CTGCTTCAAG GTGTACGGAG CTGCGTATCT ACAGAAGCTT
    CTGGAGCCGC TGTTGAGAGG AATCATCACT ACACCAGAGT GGCAGCAGAT CAGTTTTGAA
    GTGGACCCTA CTAGACTTGA ACAGGCAGAG AACCTGGAGG AGAACCAGAA GAACCTGCTT
    CAGATCACTG ACCGCTTCTT CCTGGCCATC ATCAACTCCT CGAGCGAGTT TCCTCCACAG
    CTGCGCAGTG TGTGCCACTG TCTGTATCAG GTGGTCAGTC AGCGGTTTCC TCAGAACAGC
    ATTGGTGCTG TAGGAAGTGC CATGTTCCTG CGCTTTATTA ACCCAGCTAT CGTGTCCCCA
    TATGAGGCTG GAATCCTAGA CAAGAAACCC CCACCTCGCA TCGAGCGTGG CCTTAAACTC
    ATGTCTAAGA TTCTGCAGAG CATTGCTAAT CATGTTCTCT TCACAAAAGA GGAGCATATG
    AGGCCCTTTA ATGATTTTGT CAAAAGCAAC TTCGATGCAG CCAGAAGGTT CTTCTTGGAT
    ATCGCATCTG ATTCTCCACC AAGTGATTCT GTGAACCATA GTCTGTCTTT CATCAGTGAC
    GGTAATGTGC TCGCTCTGCA CCGGCTATTA TGGAACAACC AGGAAAGAAT TGGCCAATAC
    CTGTCCAGCA ACAGGGACCA CAAGGCAGTG GGCCGTAGAC CATTTGACAA GATGGCCACT
    CTTCTGGCGT ACCTGGGTCC TCCTGAACAT AAGCCAGTAG CAGACACACA CTGGTCCAGC
    CTCAACCTCA CCAGCTCAAA GTTTGAGGAG TTCATGACAA GGCATCAAGT CCATGAGAAG
    GAGGAATTTA AAGCATTAAA AACCCTCAAC ATATTTTACC AAGCTGGCAC ATCTAAGAAC
    GGCAACCCTG TGTTTTATTA CATCGCACGC AGGTTCAAAA CTGGACAAAT CAATGGTGAT
    CTTCTGATCT ACCACGTCCT GCTGACGCTC AAGCCCTACT ATGCCAAGCA TTATGAGATC
    GTAGTTGACC TTACTCATGT GGGCCCCAGT AACCGTTTCA AGACGGACTT CTTATCCAAA
    TGGTTTGTGG TCTTTCCAAA CTTCGCCTAT GAGAACGTGG CTGCTGTGTA CATCTACAAC
    TGCAACACCT GGGTACGCGA GTACACCAAA TACCACGAGC GACTGCTGAC TGGCCTCAAG
    GGGAGCAAGA AGCTGGTGTT TATCGATTCG CCTGCACGTC TAGCTGAACA CATCGAACCC
    GAACAGCAGA AGCTGCCCGC AGCTACTTTA GCACTGGAAG AAGACCTCAA AGTGTTCCAC
    AACGCCCTAA AGCTTGCACA CAGAGACACA AAAGTGTCAA TTAAGGTGGG ATCCACAGCT
    GTACAGGTGA CCTCGGCCGA AAGAACGCGT GTCCTAGGAC AGCCTGTGTT CCTAAATGAC
    ATCTACTACG CTTCAGAGAT CGAGGAGATA TGCCTGGTGG ACGAGAACCA GTTCACGCTA
    ACCATCGCTA ACCAGGGCAC CCCACTCACT TTCATGCACC AGGAGTGTGA AGCCATCGTT
    CAGTCCATCA TCCACATCCG CACGCGCTGG GAACTTTCCC AACCAGACTC CATTCCCCAA
    CACACCAAAA TCAGGCCTAA GGATGTTCCT GGCACCCTGC TCAACATCGC TCTTCTCAAC
    CTGGGAAGCT CCGACCCCAG TCTGAGGTCT GCGGCCTACA ATCTCCTTTG TGCCTTAACC
    TGCACCTTCA ACCTGAAGAT TGAAGGCCAG CTTCTGGAAA CGTCTGGCTT GTGCATCCCA
    GCCAACAACA CGCTTTTTAT TGTGTCCATT AGCAAAACGC TAGCAGCTAA TGAACCCCAC
    CTCACTCTGG AGTTCCTGGA AGAGTGCATC TCAGGATTCA GTAAATCAAG TATAGAGCTG
    AAGCATCTGT GTCTAGAATA TATGACTCCT TGGCTCCGTA ATTTGGTGCG CTTTTGCAAA
    CACAATGATG ACGCCAAACG GCAGCGTGTC ACGGCCATCC TGGACAAGCT TATTACTATG
    ACCATCAACG AGAAGCAGAT GTACCCCTCC ATACAGGCCA AGATATGGGG CAGCTTGGGT
    CAGATCACAG ACCTGCTTGA TGTGGTGTTG GACAGCTTTA TAAAGACCAG CGCTACTGGC
    GGTTTAGGAT CCATCAAAGC TGAAGTGATG GCTGACACTG CTGTAGCTTT GGCGTCAGGA
    AATGTCAAAC TGGTCTCCAG CAAGGTGATT GGCCGAATGT GTAAGATCAT CGACAAGACG
    TGTCTGTCTC CCACTCCGAC CCTTGAGCAG CACCTCATGT GGGACGACAT CGCCATCCTG
    GCCCGGTACA TGCTCATGCT GTCCTTCAAC AACTCTCTGG ACGTGGCCGC TCATCTGCCA
    TACCTGTTCC ACGTCGTCAC CCTGCTGGTG GCCACGGGCC CTCTCTCGCT GCGTGCATCA
    ACACACGGCC TGGTCATCAA CATCATTCAC TCGCTCTGCA CATGCTCACA ACTCAACTTC
    AGTGAGGAGA CTAAACAGGT GCTGCGCCTG AGTCTGACTG AGTTCTCCCT GCCCAAGTTC
    TACCTGCTGT TCGGCATCAG TAAGGTCAAA TCTGCGGCGG TCATCGCTTT CCGTTCCAGC
    TACAGAGACC GCTCATTCTC TCCAGGCTCT TATGAGAGAG AGACATTTGC TCTGTCTTCC
    CTGGAAACAG TCACGGAGGC TCTTTTGGAG ATCATGGAGG CCTGCATGAG GGACATTCCT
    GGATGCAAAT GGCTAGATCA GTGGACTGAA TTGGCGCAAA AGTTTGCGTT CCAGTATAAC
    CCCTCTCTTC AGCCTCGGGC GCTGGTGGTG TTCGGCTGCA TCAGTAAACG AGTGACTCAC
    GGACAGCTAA AGCAGATCAT TCGTATTCTC AGTAAAGCCA GTCCTCTACT CAGCATTGAT
    CGGGGGCTGG AGAGCTGCTT AAAGGGCCCA GATAATTACA ACAGCCAGGT TCTCATCGAA
    GCCACAGTCA TCGCTCTCAC CAAACTACAG CCTCTCCTCG ATCAGGATTC TCCTATGCAT
    AAAGCTCTGT TCTGGGTGGC CGTGGCCGTG CTTCAGTTGG ATGAAGTGAA TCTGTACTCC
    GCCGGCACTG CCTTACTGGA GCAGAACCTG CACACACTGG ACAGCCTCCG AGTGTTTAAT
    GATAAGAGTC CTGAAGAGGT TTTTATGGAG ATCCGGAAAC CTCTAGAGTG GCACTGTAAA
    CAAATGGACC ATTTTGTGGG ACTCAACTTT GCCTCCAACT TCAATTTTGC ATTAGTGGGC
    CATTTACTTA AAGGATACAG ACATCCCTCT TCAACAACAG TTGCCAGGAC AGTTCGTATC
    CTGCATACAC TCCTGGGCCT GGTGGGAAAA CACCGCAGCT GTGACAAGTT TGAGGTGAAC
    ACGCAGAGTG TGGCCTATCT GGCAGCTTTG CTCACAGTGT CAGAGGAAGT TAGGAGCCGC
    TGCAGTTTAA AGCACAGGAA ATCATTGCTG CTTTCAGAAG TTACAATGGA GAACTTCCCC
    ATGGACACAT ACACCGTTTA CGACACTGAA CCCGGGTGCA GGCTACTGCG TGAAAACCAG
    CCTTGGGCTT CTCCGAAAGT GTCAGAGCGC AGTCTTGCAC CTCCGTACTC CACAATAGGG
    CAGACCAGCC TTCGTCCACG CAAATCCATG AGTGTAGACA TGGGCCAGCC GTCTCAGGCC
    AATGCGAAGA AACTACTGGG TATTAGCGGC ACTAGAAAGA GTTTCGATCA CTTAATATCA
    GAATCCAAAG CCCCAAAAAG ACCAGACATG GAGTCGGGCA TCACCACACC TCCAAAGATG
    AGGCGGGTTT CTGAAAATGA CTATGAGATC GAAACTCAGC GAATAGCCAA CTCTCATTTA
    CGCAAAGTGT CTGTGTCTGA ATCCAATGTC CTCCTGGACG AGAACGTACT TACGGACCCC
    AAGATCCAGA CGTTGCTCCT CACTGTACTG GCAACACTGG TGAAGTACAC TACAGACGAG
    TTTCATCAGC GGATCTTGTA TGAGTATTTG GCTGAGGCCA GTGTGGTCTT CCCCAAGGTC
    TTTCCAGTTG TGCACAACCT GCTGGACTCC AAGTTAAGCA CCGTCCTTTC CATGTGCCAG
    GACCCGAACC TTCTGAACCC CGTCCATGGC ATCATTCAGA ACGTGGTCTA TCATGAGGAG
    TCTCCCCCTC AGTACCAGCC GTCCTGCTTG CAGAGCTTTG GTTTCAATGG TCTTTGGAGG
    TTTGCCGGGC CGTTCTCTAA GCAAACCCAA ATTCCAGACT ACGCCGAGCT GGTGGTTAAA
    TTTTTGGATG CTTTGATTGA CACGTATCTC CCGGGCATTG AGGAGGAAGG AGGAGAAGAG
    TCACTGCGGA CCCCGACCTC GCCATATCCA CCAACCGTCC AGAGCCAGCT GAGCATCACA
    GCCAATCTCA ACCTGTCCAA CTCCATGACA TCACTTGCCA CATCACAGCA CTCCCCAGAG
    GGCCCTATAG TTTATAGACG GTCCCCAACT CTCTCCCATC CACGAACTAC AGAGGAGGTG
    CCACCGAAAA CAGCTGCCTG TCTGCCTTGC TCTAAGTCGG CTGTATTCCC ACCTCACCTC
    GTCCTGCCAG GCATCGACAA GGAGAACGTT GAGATTTCTC CCACCTCGAG TCTGTCCACC
    AGTGGGCGAA TGCGGCACGG CTCAGCAAGC CAGGTGCAGA AGCAGCGCAG CGCAGGCAGC
    TTCAAACGGC CAAGCATCAA AAAGATCGTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa39873
    Clone ID Related Accession (Same CDS sequence) XM_009291764.2 , XM_009291764.3
    Accession Version XM_009291764.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8433bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product neurofibromin isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291764.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGGCGGCGC ACAAGCCCGT GGAATGGGTC CAGGCCATCA TTAACAGATT CGACGAACAG 
    CTCCCAATCA AAACAGGACA GCAGAGCACC CACACCAAAG TGAGTACAGA GCACAACAAA
    GAATGTTTGA TCAACATCTC CAAGTACAAA TTTGCCCTCG TCATCAATGG CCTGACCAAC
    ATACTCAAAA ATGTGAACAA CATGCGGATA TTTGGCGAAG CTGCTGAGAA GAACCTCTAC
    TTCTCCCAGC TGATCATACT GGACACACTG GAGAAGTGTC TAGCAGGGCA ACCTAAGGAT
    TGCCTGAGGC TGGATGAGGC TATGTTGGTA AAACAGTTGC TGCCTGAAAT CTGTCACTTC
    CTGCACTCGT ACCGTGAGGG CCAGCAACAC GCCACTCAGC TGCGCAGCTC CGCCTCTGCT
    GTGCTTTTCT CTCTCAGCTG CAACAACTTT AATGCCGTCT TCAGTCGTAT CTCCACCAGG
    TTGCAAGAGC TCACAGTTTG TACAGAGGAC ACAGTGGATG TCCATGACAT TGAGCTCATT
    CAGTATATCA ATGTAGATTG CTCCAAGTTA AAGAGACTCC TACAAGAAGC AGTACTTAAG
    TTCAAGTCTT TGAAAAAGCC GGCTCAGCTT GCAGTAATCA GCAGTTTGGA AAAGGCTTTC
    TGGAACTGGG TTGAACACTA CCCTGATGAT TTCACTATGC TCTATCAGAG ACCCCAGGCC
    GACATGGCCG ACTGTGCAGA GAGACTGTTC GACCTAGTGG ACAGTTTTGC GGAAAGCGCA
    AAGAGAAAAG CTGCTGTGTG GCCACTGCAG ATCATCCTCC TCATCCTCTG TCCAGAGATC
    ACGCAGAGCA TCACCAAAGA AGTAGGGGGA GAGGAGAAAG CTAACAAGAA GTCATTTGTA
    GACAGTTTAC GAAAGGCTCT TGGCCAACAC AGCGGCAGCA AACAATTGAC GGAAAGTGCA
    GCTGTTGCAT GCGTCAAACT CTGCAAAGCC TGCACATATA TCAACTGGGA GGATAATTCT
    GTCATCTTTC TCCTGGTTCA GTCCATCGTC ATGGACCTTA AGGGTCTCCT GTTTAACCCC
    ACCAAACCTT TCTCAAGAGG TACGGGCTGC CAGAGCGCCG ATGTAGATTT GATGATCGAC
    TGCTTTGTGT CATGTTATCG CATCAATCCT CGCAACAACC AGCATTTTAA GGTGTGTCTG
    GCCTCGACTT CTCCTCCTAC CTTCCATTTC GTCCTTGTCA ACTCCCTGCA CAAAATTATC
    ACAAATTCTC ACCTGGACTG GTGGCCGAAG ATTGACGCCA TCTACTGCTA CTCAGGAGAA
    CTACGCACCA TGTTTGCAGA CACACTGGGT CGAGTGATTC AGAGTCTCAG TGCACATCCG
    CCTCTGAGGA TGACATCAAG TTTGACATTC AAAGAGAAAA TGACAACAAG TTTGAAGTTC
    AAGGAAAAGT CCACAGATCT TGATTCGCGT TCTTTGCTGC TGGCACTGGT CAAACTTATC
    CACGCTGACC CCAAACTAAT GCTGCACAAT CCGGGAAAAC CTGGTCAGGA GATTCAGAAC
    AGCACAGCTG AGATCATCAA AGGTCTGGTT CAGCTGGTTC CTCTTACCCA CAGTGCTGAG
    CTTTCCCAAG AGGCCATGGA GGCATTGCTT GTTTTACATC AGCCAGAAAC CATTGAGCTA
    TGGAACCCGG ACGCTCCCAT TGAGACCTTC TGGGACATCA GCTCTCAGGT GTTATTTCTA
    ATTTGTAAGA AGCTGATTGG ACACCAGATG ATCAACAGTA CAGAGATACT CAAGTGGCTC
    AGGGAGATTC TCATATGCAG GAACAAGTTT CTATTAAAGA ACAAGGAGTG TGCTACACAG
    GGCAGTCTGA TCCCCATCAG CAGACAGGCT CAGACCAAGC TGGAGGTGTG TCTGTACACG
    TTCCTGTGGA GTCCTGATGC AGAGGCTGTT TTGGTGGCCA TGTCCTGTTT CAGGCATCTC
    TGTGAGGAGG CCGATATTCG CTGCAGTGTC GATGAGCTGC CTGTTCACAC AGTCCTACCC
    AATTACAACA CCTTCATTGA GCTTGCATCC GTCAGCAATA TGATGGCCAC AGGTCGCAAC
    ACACTACAAA AAAGAGTGAT GGCACTTTTA AGAAGAATAA AGCACCCTAC AGCTGGAAAT
    GTAGAGGCAT GGGAAGACAC ATATGCAAAG TGGGAGCAAG CTACAAAACA GATACTCAAC
    CACCCAAAAA ACAAAGTGGA AGATGGACAG GTAGTTAACA TAAGATTGGT CAATTATTTC
    ATCTTTTTTT CCATTCATGG TCATCTATTT TCTGTTCGTC CTCAGGAGTG GATCAACATG
    ACTGGTTTCT TGTGCGCTCT GGGAGGTGTT TGTCTCCAAC AGCGCAGCAC GCCGAGTCTG
    GGCACCTACA GTCCTCCAAT GGGCCCCCTG ACCGAACGCA AGGGGTCCAT GATTTCCATG
    AGCTCCTGCG AGGTAAACAG CGAAACATCC CTGAATCGCT TTCTGGAGCG TCTGCTGGCT
    CTGATGGTCT GCACACATGA GAAAGTGGGA GTGCAAATTC GCACCAACGT CAAAGACCTG
    GTGGGACTGG AGCTCAGTCC GGCTCTCTAT TCAATGCTCT TCACTAAGCT GCGCAACAGC
    ATCGGACGCT TCTTTGACAC CCAGGGGCCG GTTCCTATTA CAGATGCAAA CACTCTGTTT
    GTAGAGCAGA CCATTGCTAT CATGAAGAAC TTGCTGGACA GTCACACTGA AGGAAGCTCA
    GAGCACCTTG GTCAAGCCAG CATTGAGACC ATGATGCTCA ATCTAGTTAG GTATGTGCGT
    ATTTTAGGGA ACATTGTTCA TGCCATTCAG ATCAAAACCA AGTTGTGTCA GTTAGTGGAG
    GTGATGATGG AGCGCAGGGA TGACCTCTCC TTCTGCCAGG AGATGAAATT CAGGAATAAA
    ATGGTGGAGT ACCTCACAGA CTGGGTGATG GGAACCTCCA ATCAAGCTGC GGACGATGAT
    GTCAAATGCC TGACCCGCGA TCTGGACCAG GCCAGTATGG AGGCTGTCGT GTCTCTGTTG
    GCTGGTCTTC CTCTACAGCC TGAGGAGGGT GACGGTGTTG AGCTCATGGA GGCCAAATCA
    CAGCTCTTCC TGAAGTACTT CACACTGTTC ATGAACCTGT TGAATGACTG CAGCGAGGTT
    GAGGAAGATG GCCAGCAGGT CGCAGGGAGG AAACGTGGCA TGTCTAGACG ACTGGCATCT
    CTGCGGCACT GCACAGTATT GGCTATGTCT AACTTGCTCA ATGCTAATGT GGATAGCGGC
    CTCATGCACT CAATCGGTCT GGGATACCAT AAGGACCTGC AGACACGGGC TACATTTATG
    GAAGTGCTCA CTAAGATCCT TCAGCAAGGG ACGGAGTTCG ACACGCTTGC AGAGACTGTG
    CTTGCAGATC GCTTTGAGAG ACTGGTCGAG CTGGTCACCA TGATGGGAGA TCAGGGAGAG
    CTGCCCATAG CCATGGCTCT GGCCAATGTG GTCCCATGCT CTCAATGGGA TGAGCTGGCC
    AGAGTGTTAG TGACTCTCTT CGACTCTCGC CATCTGCTGT ATCAGCTGTT GTGGAATATG
    TTCTCCAAAG AGGTGGAGCT GGCAGACTCA ATGCAGACGC TCTTTAGAGG AAATAGCCTA
    GCCAGCAAGA TTATGACCTT CTGCTTCAAG GTGTACGGAG CTGCGTATCT ACAGAAGCTT
    CTGGAGCCGC TGTTGAGAGG AATCATCACT ACACCAGAGT GGCAGCAGAT CAGTTTTGAA
    GTGGACCCTA CTAGACTTGA ACAGGCAGAG AACCTGGAGG AGAACCAGAA GAACCTGCTT
    CAGATCACTG ACCGCTTCTT CCTGGCCATC ATCAACTCCT CGAGCGAGTT TCCTCCACAG
    CTGCGCAGTG TGTGCCACTG TCTGTATCAG GTGGTCAGTC AGCGGTTTCC TCAGAACAGC
    ATTGGTGCTG TAGGAAGTGC CATGTTCCTG CGCTTTATTA ACCCAGCTAT CGTGTCCCCA
    TATGAGGCTG GAATCCTAGA CAAGAAACCC CCACCTCGCA TCGAGCGTGG CCTTAAACTC
    ATGTCTAAGA TTCTGCAGAG CATTGCTAAT CATGTTCTCT TCACAAAAGA GGAGCATATG
    AGGCCCTTTA ATGATTTTGT CAAAAGCAAC TTCGATGCAG CCAGAAGGTT CTTCTTGGAT
    ATCGCATCTG ATTCTCCACC AAGTGATTCT GTGAACCATA GTCTGTCTTT CATCAGTGAC
    GGTAATGTGC TCGCTCTGCA CCGGCTATTA TGGAACAACC AGGAAAGAAT TGGCCAATAC
    CTGTCCAGCA ACAGGGACCA CAAGGCAGTG GGCCGTAGAC CATTTGACAA GATGGCCACT
    CTTCTGGCGT ACCTGGGTCC TCCTGAACAT AAGCCAGTAG CAGACACACA CTGGTCCAGC
    CTCAACCTCA CCAGCTCAAA GTTTGAGGAG TTCATGACAA GGCATCAAGT CCATGAGAAG
    GAGGAATTTA AAGCATTAAA AACCCTCAAC ATATTTTACC AAGCTGGCAC ATCTAAGAAC
    GGCAACCCTG TGTTTTATTA CATCGCACGC AGGTTCAAAA CTGGACAAAT CAATGGTGAT
    CTTCTGATCT ACCACGTCCT GCTGACGCTC AAGCCCTACT ATGCCAAGCA TTATGAGATC
    GTAGTTGACC TTACTCATGT GGGCCCCAGT AACCGTTTCA AGACGGACTT CTTATCCAAA
    TGGTTTGTGG TCTTTCCAAA CTTCGCCTAT GAGAACGTGG CTGCTGTGTA CATCTACAAC
    TGCAACACCT GGGTACGCGA GTACACCAAA TACCACGAGC GACTGCTGAC TGGCCTCAAG
    GGGAGCAAGA AGCTGGTGTT TATCGATTCG CCTGCACGTC TAGCTGAACA CATCGAACCC
    GAACAGCAGA AGCTGCCCGC AGCTACTTTA GCACTGGAAG AAGACCTCAA AGTGTTCCAC
    AACGCCCTAA AGCTTGCACA CAGAGACACA AAAGTGTCAA TTAAGGTGGG ATCCACAGCT
    GTACAGGTGA CCTCGGCCGA AAGAACGCGT GTCCTAGGAC AGCCTGTGTT CCTAAATGAC
    ATCTACTACG CTTCAGAGAT CGAGGAGATA TGCCTGGTGG ACGAGAACCA GTTCACGCTA
    ACCATCGCTA ACCAGGGCAC CCCACTCACT TTCATGCACC AGGAGTGTGA AGCCATCGTT
    CAGTCCATCA TCCACATCCG CACGCGCTGG GAACTTTCCC AACCAGACTC CATTCCCCAA
    CACACCAAAA TCAGGCCTAA GGATGTTCCT GGCACCCTGC TCAACATCGC TCTTCTCAAC
    CTGGGAAGCT CCGACCCCAG TCTGAGGTCT GCGGCCTACA ATCTCCTTTG TGCCTTAACC
    TGCACCTTCA ACCTGAAGAT TGAAGGCCAG CTTCTGGAAA CGTCTGGCTT GTGCATCCCA
    GCCAACAACA CGCTTTTTAT TGTGTCCATT AGCAAAACGC TAGCAGCTAA TGAACCCCAC
    CTCACTCTGG AGTTCCTGGA AGAGTGCATC TCAGGATTCA GTAAATCAAG TATAGAGCTG
    AAGCATCTGT GTCTAGAATA TATGACTCCT TGGCTCCGTA ATTTGGTGCG CTTTTGCAAA
    CACAATGATG ACGCCAAACG GCAGCGTGTC ACGGCCATCC TGGACAAGCT TATTACTATG
    ACCATCAACG AGAAGCAGAT GTACCCCTCC ATACAGGCCA AGATATGGGG CAGCTTGGGT
    CAGATCACAG ACCTGCTTGA TGTGGTGTTG GACAGCTTTA TAAAGACCAG CGCTACTGGC
    GGTTTAGGAT CCATCAAAGC TGAAGTGATG GCTGACACTG CTGTAGCTTT GGCGTCAGGA
    AATGTCAAAC TGGTCTCCAG CAAGGTGATT GGCCGAATGT GTAAGATCAT CGACAAGACG
    TGTCTGTCTC CCACTCCGAC CCTTGAGCAG CACCTCATGT GGGACGACAT CGCCATCCTG
    GCCCGGTACA TGCTCATGCT GTCCTTCAAC AACTCTCTGG ACGTGGCCGC TCATCTGCCA
    TACCTGTTCC ACGTCGTCAC CCTGCTGGTG GCCACGGGCC CTCTCTCGCT GCGTGCATCA
    ACACACGGCC TGGTCATCAA CATCATTCAC TCGCTCTGCA CATGCTCACA ACTCAACTTC
    AGTGAGGAGA CTAAACAGGT GCTGCGCCTG AGTCTGACTG AGTTCTCCCT GCCCAAGTTC
    TACCTGCTGT TCGGCATCAG TAAGGTCAAA TCTGCGGCGG TCATCGCTTT CCGTTCCAGC
    TACAGAGACC GCTCATTCTC TCCAGGCTCT TATGAGAGAG AGACATTTGC TCTGTCTTCC
    CTGGAAACAG TCACGGAGGC TCTTTTGGAG ATCATGGAGG CCTGCATGAG GGACATTCCT
    GGATGCAAAT GGCTAGATCA GTGGACTGAA TTGGCGCAAA AGTTTGCGTT CCAGTATAAC
    CCCTCTCTTC AGCCTCGGGC GCTGGTGGTG TTCGGCTGCA TCAGTAAACG AGTGACTCAC
    GGACAGCTAA AGCAGATCAT TCGTATTCTC AGTAAAGCCA GTCCTCTACT CAGCATTGAT
    CGGGGGCTGG AGAGCTGCTT AAAGGGCCCA GATAATTACA ACAGCCAGGT TCTCATCGAA
    GCCACAGTCA TCGCTCTCAC CAAACTACAG CCTCTCCTCG ATCAGGATTC TCCTATGCAT
    AAAGCTCTGT TCTGGGTGGC CGTGGCCGTG CTTCAGTTGG ATGAAGTGAA TCTGTACTCC
    GCCGGCACTG CCTTACTGGA GCAGAACCTG CACACACTGG ACAGCCTCCG AGTGTTTAAT
    GATAAGAGTC CTGAAGAGGT TTTTATGGAG ATCCGGAAAC CTCTAGAGTG GCACTGTAAA
    CAAATGGACC ATTTTGTGGG ACTCAACTTT GCCTCCAACT TCAATTTTGC ATTAGTGGGC
    CATTTACTTA AAGGATACAG ACATCCCTCT TCAACAACAG TTGCCAGGAC AGTTCGTATC
    CTGCATACAC TCCTGGGCCT GGTGGGAAAA CACCGCAGCT GTGACAAGTT TGAGGTGAAC
    ACGCAGAGTG TGGCCTATCT GGCAGCTTTG CTCACAGTGT CAGAGGAAGT TAGGAGCCGC
    TGCAGTTTAA AGCACAGGAA ATCATTGCTG CTTTCAGAAG TTACAATGGA GAACTTCCCC
    ATGGACACAT ACACCGTTTA CGACACTGAA CCCGGGTGCA GGCTACTGCG TGAAAACCAG
    CCTTGGGCTT CTCCGAAAGT GTCAGAGCGC AGTCTTGCAC CTCCGTACTC CACAATAGGG
    CAGACCAGCC TTCGTCCACG CAAATCCATG AGTGTAGACA TGGGCCAGCC GTCTCAGGCC
    AATGCGAAGA AACTACTGGG TATTAGCGGC ACTAGAAAGA GTTTCGATCA CTTAATATCA
    GAATCCAAAG CCCCAAAAAG ACCAGACATG GAGTCGGGCA TCACCACACC TCCAAAGATG
    AGGCGGGTTT CTGAAAATGA CTATGAGATC GAAACTCAGC GAATAGCCAA CTCTCATTTA
    CGCAAAGTGT CTGTGTCTGA ATCCAATGTC CTCCTGGACG AGAACGTACT TACGGACCCC
    AAGATCCAGA CGTTGCTCCT CACTGTACTG GCAACACTGG TGAAGTACAC TACAGACGAG
    TTTCATCAGC GGATCTTGTA TGAGTATTTG GCTGAGGCCA GTGTGGTCTT CCCCAAGGTC
    TTTCCAGTTG TGCACAACCT GCTGGACTCC AAGTTAAGCA CCGTCCTTTC CATGTGCCAG
    GACCCGAACC TTCTGAACCC CGTCCATGGC ATCATTCAGA ACGTGGTCTA TCATGAGGAG
    TCTCCCCCTC AGTACCAGCC GTCCTGCTTG CAGAGCTTTG GTTTCAATGG TCTTTGGAGG
    TTTGCCGGGC CGTTCTCTAA GCAAACCCAA ATTCCAGACT ACGCCGAGCT GGTGGTTAAA
    TTTTTGGATG CTTTGATTGA CACGTATCTC CCGGGCATTG AGGAGGAAGG AGGAGAAGAG
    TCACTGCGGA CCCCGACCTC GCCATATCCA CCAACCGTCC AGAGCCAGCT GAGCATCACA
    GCCAATCTCA ACCTGTCCAA CTCCATGACA TCACTTGCCA CATCACAGCA CTCCCCAGAG
    GGCCCTATAG TTTATAGACG GTCCCCAACT CTCTCCCATC CACGAACTAC AGAGGAGGTG
    CCACCGAAAA CAGCTGCCTG TCTGCCTTGC TCTAAGTCGG CTGTATTCCC ACCTCACCTC
    GTCCTGCCAG GCATCGACAA GGAGAACGTT GAGATTTCTC CCACCTCGAG TCTGTCCACC
    AGTGGGCGAA TGCGGCACGG CTCAGCAAGC CAGGTGCAGA AGCAGCGCAG CGCAGGCAGC
    TTCAAACGGC CAAGCATCAA AAAGATCGTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290039.1
    CDS247..8679
    Translation

    Target ORF information:

    RefSeq Version XM_009291764.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio neurofibromin 1a (nf1a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009291764.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGGCGGCGC ACAAGCCCGT GGAATGGGTC CAGGCCATCA TTAACAGATT CGACGAACAG 
    CTCCCAATCA AAACAGGACA GCAGAGCACC CACACCAAAG TGAGTACAGA GCACAACAAA
    GAATGTTTGA TCAACATCTC CAAGTACAAA TTTGCCCTCG TCATCAATGG CCTGACCAAC
    ATACTCAAAA ATGTGAACAA CATGCGGATA TTTGGCGAAG CTGCTGAGAA GAACCTCTAC
    TTCTCCCAGC TGATCATACT GGACACACTG GAGAAGTGTC TAGCAGGGCA ACCTAAGGAT
    TGCCTGAGGC TGGATGAGGC TATGTTGGTA AAACAGTTGC TGCCTGAAAT CTGTCACTTC
    CTGCACTCGT ACCGTGAGGG CCAGCAACAC GCCACTCAGC TGCGCAGCTC CGCCTCTGCT
    GTGCTTTTCT CTCTCAGCTG CAACAACTTT AATGCCGTCT TCAGTCGTAT CTCCACCAGG
    TTGCAAGAGC TCACAGTTTG TACAGAGGAC ACAGTGGATG TCCATGACAT TGAGCTCATT
    CAGTATATCA ATGTAGATTG CTCCAAGTTA AAGAGACTCC TACAAGAAGC AGTACTTAAG
    TTCAAGTCTT TGAAAAAGCC GGCTCAGCTT GCAGTAATCA GCAGTTTGGA AAAGGCTTTC
    TGGAACTGGG TTGAACACTA CCCTGATGAT TTCACTATGC TCTATCAGAG ACCCCAGGCC
    GACATGGCCG ACTGTGCAGA GAGACTGTTC GACCTAGTGG ACAGTTTTGC GGAAAGCGCA
    AAGAGAAAAG CTGCTGTGTG GCCACTGCAG ATCATCCTCC TCATCCTCTG TCCAGAGATC
    ACGCAGAGCA TCACCAAAGA AGTAGGGGGA GAGGAGAAAG CTAACAAGAA GTCATTTGTA
    GACAGTTTAC GAAAGGCTCT TGGCCAACAC AGCGGCAGCA AACAATTGAC GGAAAGTGCA
    GCTGTTGCAT GCGTCAAACT CTGCAAAGCC TGCACATATA TCAACTGGGA GGATAATTCT
    GTCATCTTTC TCCTGGTTCA GTCCATCGTC ATGGACCTTA AGGGTCTCCT GTTTAACCCC
    ACCAAACCTT TCTCAAGAGG TACGGGCTGC CAGAGCGCCG ATGTAGATTT GATGATCGAC
    TGCTTTGTGT CATGTTATCG CATCAATCCT CGCAACAACC AGCATTTTAA GGTGTGTCTG
    GCCTCGACTT CTCCTCCTAC CTTCCATTTC GTCCTTGTCA ACTCCCTGCA CAAAATTATC
    ACAAATTCTC ACCTGGACTG GTGGCCGAAG ATTGACGCCA TCTACTGCTA CTCAGGAGAA
    CTACGCACCA TGTTTGCAGA CACACTGGGT CGAGTGATTC AGAGTCTCAG TGCACATCCG
    CCTCTGAGGA TGACATCAAG TTTGACATTC AAAGAGAAAA TGACAACAAG TTTGAAGTTC
    AAGGAAAAGT CCACAGATCT TGATTCGCGT TCTTTGCTGC TGGCACTGGT CAAACTTATC
    CACGCTGACC CCAAACTAAT GCTGCACAAT CCGGGAAAAC CTGGTCAGGA GATTCAGAAC
    AGCACAGCTG AGATCATCAA AGGTCTGGTT CAGCTGGTTC CTCTTACCCA CAGTGCTGAG
    CTTTCCCAAG AGGCCATGGA GGCATTGCTT GTTTTACATC AGCCAGAAAC CATTGAGCTA
    TGGAACCCGG ACGCTCCCAT TGAGACCTTC TGGGACATCA GCTCTCAGGT GTTATTTCTA
    ATTTGTAAGA AGCTGATTGG ACACCAGATG ATCAACAGTA CAGAGATACT CAAGTGGCTC
    AGGGAGATTC TCATATGCAG GAACAAGTTT CTATTAAAGA ACAAGGAGTG TGCTACACAG
    GGCAGTCTGA TCCCCATCAG CAGACAGGCT CAGACCAAGC TGGAGGTGTG TCTGTACACG
    TTCCTGTGGA GTCCTGATGC AGAGGCTGTT TTGGTGGCCA TGTCCTGTTT CAGGCATCTC
    TGTGAGGAGG CCGATATTCG CTGCAGTGTC GATGAGCTGC CTGTTCACAC AGTCCTACCC
    AATTACAACA CCTTCATTGA GCTTGCATCC GTCAGCAATA TGATGGCCAC AGGTCGCAAC
    ACACTACAAA AAAGAGTGAT GGCACTTTTA AGAAGAATAA AGCACCCTAC AGCTGGAAAT
    GTAGAGGCAT GGGAAGACAC ATATGCAAAG TGGGAGCAAG CTACAAAACA GATACTCAAC
    CACCCAAAAA ACAAAGTGGA AGATGGACAG GTAGTTAACA TAAGATTGGT CAATTATTTC
    ATCTTTTTTT CCATTCATGG TCATCTATTT TCTGTTCGTC CTCAGGAGTG GATCAACATG
    ACTGGTTTCT TGTGCGCTCT GGGAGGTGTT TGTCTCCAAC AGCGCAGCAC GCCGAGTCTG
    GGCACCTACA GTCCTCCAAT GGGCCCCCTG ACCGAACGCA AGGGGTCCAT GATTTCCATG
    AGCTCCTGCG AGGTAAACAG CGAAACATCC CTGAATCGCT TTCTGGAGCG TCTGCTGGCT
    CTGATGGTCT GCACACATGA GAAAGTGGGA GTGCAAATTC GCACCAACGT CAAAGACCTG
    GTGGGACTGG AGCTCAGTCC GGCTCTCTAT TCAATGCTCT TCACTAAGCT GCGCAACAGC
    ATCGGACGCT TCTTTGACAC CCAGGGGCCG GTTCCTATTA CAGATGCAAA CACTCTGTTT
    GTAGAGCAGA CCATTGCTAT CATGAAGAAC TTGCTGGACA GTCACACTGA AGGAAGCTCA
    GAGCACCTTG GTCAAGCCAG CATTGAGACC ATGATGCTCA ATCTAGTTAG GTATGTGCGT
    ATTTTAGGGA ACATTGTTCA TGCCATTCAG ATCAAAACCA AGTTGTGTCA GTTAGTGGAG
    GTGATGATGG AGCGCAGGGA TGACCTCTCC TTCTGCCAGG AGATGAAATT CAGGAATAAA
    ATGGTGGAGT ACCTCACAGA CTGGGTGATG GGAACCTCCA ATCAAGCTGC GGACGATGAT
    GTCAAATGCC TGACCCGCGA TCTGGACCAG GCCAGTATGG AGGCTGTCGT GTCTCTGTTG
    GCTGGTCTTC CTCTACAGCC TGAGGAGGGT GACGGTGTTG AGCTCATGGA GGCCAAATCA
    CAGCTCTTCC TGAAGTACTT CACACTGTTC ATGAACCTGT TGAATGACTG CAGCGAGGTT
    GAGGAAGATG GCCAGCAGGT CGCAGGGAGG AAACGTGGCA TGTCTAGACG ACTGGCATCT
    CTGCGGCACT GCACAGTATT GGCTATGTCT AACTTGCTCA ATGCTAATGT GGATAGCGGC
    CTCATGCACT CAATCGGTCT GGGATACCAT AAGGACCTGC AGACACGGGC TACATTTATG
    GAAGTGCTCA CTAAGATCCT TCAGCAAGGG ACGGAGTTCG ACACGCTTGC AGAGACTGTG
    CTTGCAGATC GCTTTGAGAG ACTGGTCGAG CTGGTCACCA TGATGGGAGA TCAGGGAGAG
    CTGCCCATAG CCATGGCTCT GGCCAATGTG GTCCCATGCT CTCAATGGGA TGAGCTGGCC
    AGAGTGTTAG TGACTCTCTT CGACTCTCGC CATCTGCTGT ATCAGCTGTT GTGGAATATG
    TTCTCCAAAG AGGTGGAGCT GGCAGACTCA ATGCAGACGC TCTTTAGAGG AAATAGCCTA
    GCCAGCAAGA TTATGACCTT CTGCTTCAAG GTGTACGGAG CTGCGTATCT ACAGAAGCTT
    CTGGAGCCGC TGTTGAGAGG AATCATCACT ACACCAGAGT GGCAGCAGAT CAGTTTTGAA
    GTGGACCCTA CTAGACTTGA ACAGGCAGAG AACCTGGAGG AGAACCAGAA GAACCTGCTT
    CAGATCACTG ACCGCTTCTT CCTGGCCATC ATCAACTCCT CGAGCGAGTT TCCTCCACAG
    CTGCGCAGTG TGTGCCACTG TCTGTATCAG GTGGTCAGTC AGCGGTTTCC TCAGAACAGC
    ATTGGTGCTG TAGGAAGTGC CATGTTCCTG CGCTTTATTA ACCCAGCTAT CGTGTCCCCA
    TATGAGGCTG GAATCCTAGA CAAGAAACCC CCACCTCGCA TCGAGCGTGG CCTTAAACTC
    ATGTCTAAGA TTCTGCAGAG CATTGCTAAT CATGTTCTCT TCACAAAAGA GGAGCATATG
    AGGCCCTTTA ATGATTTTGT CAAAAGCAAC TTCGATGCAG CCAGAAGGTT CTTCTTGGAT
    ATCGCATCTG ATTCTCCACC AAGTGATTCT GTGAACCATA GTCTGTCTTT CATCAGTGAC
    GGTAATGTGC TCGCTCTGCA CCGGCTATTA TGGAACAACC AGGAAAGAAT TGGCCAATAC
    CTGTCCAGCA ACAGGGACCA CAAGGCAGTG GGCCGTAGAC CATTTGACAA GATGGCCACT
    CTTCTGGCGT ACCTGGGTCC TCCTGAACAT AAGCCAGTAG CAGACACACA CTGGTCCAGC
    CTCAACCTCA CCAGCTCAAA GTTTGAGGAG TTCATGACAA GGCATCAAGT CCATGAGAAG
    GAGGAATTTA AAGCATTAAA AACCCTCAAC ATATTTTACC AAGCTGGCAC ATCTAAGAAC
    GGCAACCCTG TGTTTTATTA CATCGCACGC AGGTTCAAAA CTGGACAAAT CAATGGTGAT
    CTTCTGATCT ACCACGTCCT GCTGACGCTC AAGCCCTACT ATGCCAAGCA TTATGAGATC
    GTAGTTGACC TTACTCATGT GGGCCCCAGT AACCGTTTCA AGACGGACTT CTTATCCAAA
    TGGTTTGTGG TCTTTCCAAA CTTCGCCTAT GAGAACGTGG CTGCTGTGTA CATCTACAAC
    TGCAACACCT GGGTACGCGA GTACACCAAA TACCACGAGC GACTGCTGAC TGGCCTCAAG
    GGGAGCAAGA AGCTGGTGTT TATCGATTCG CCTGCACGTC TAGCTGAACA CATCGAACCC
    GAACAGCAGA AGCTGCCCGC AGCTACTTTA GCACTGGAAG AAGACCTCAA AGTGTTCCAC
    AACGCCCTAA AGCTTGCACA CAGAGACACA AAAGTGTCAA TTAAGGTGGG ATCCACAGCT
    GTACAGGTGA CCTCGGCCGA AAGAACGCGT GTCCTAGGAC AGCCTGTGTT CCTAAATGAC
    ATCTACTACG CTTCAGAGAT CGAGGAGATA TGCCTGGTGG ACGAGAACCA GTTCACGCTA
    ACCATCGCTA ACCAGGGCAC CCCACTCACT TTCATGCACC AGGAGTGTGA AGCCATCGTT
    CAGTCCATCA TCCACATCCG CACGCGCTGG GAACTTTCCC AACCAGACTC CATTCCCCAA
    CACACCAAAA TCAGGCCTAA GGATGTTCCT GGCACCCTGC TCAACATCGC TCTTCTCAAC
    CTGGGAAGCT CCGACCCCAG TCTGAGGTCT GCGGCCTACA ATCTCCTTTG TGCCTTAACC
    TGCACCTTCA ACCTGAAGAT TGAAGGCCAG CTTCTGGAAA CGTCTGGCTT GTGCATCCCA
    GCCAACAACA CGCTTTTTAT TGTGTCCATT AGCAAAACGC TAGCAGCTAA TGAACCCCAC
    CTCACTCTGG AGTTCCTGGA AGAGTGCATC TCAGGATTCA GTAAATCAAG TATAGAGCTG
    AAGCATCTGT GTCTAGAATA TATGACTCCT TGGCTCCGTA ATTTGGTGCG CTTTTGCAAA
    CACAATGATG ACGCCAAACG GCAGCGTGTC ACGGCCATCC TGGACAAGCT TATTACTATG
    ACCATCAACG AGAAGCAGAT GTACCCCTCC ATACAGGCCA AGATATGGGG CAGCTTGGGT
    CAGATCACAG ACCTGCTTGA TGTGGTGTTG GACAGCTTTA TAAAGACCAG CGCTACTGGC
    GGTTTAGGAT CCATCAAAGC TGAAGTGATG GCTGACACTG CTGTAGCTTT GGCGTCAGGA
    AATGTCAAAC TGGTCTCCAG CAAGGTGATT GGCCGAATGT GTAAGATCAT CGACAAGACG
    TGTCTGTCTC CCACTCCGAC CCTTGAGCAG CACCTCATGT GGGACGACAT CGCCATCCTG
    GCCCGGTACA TGCTCATGCT GTCCTTCAAC AACTCTCTGG ACGTGGCCGC TCATCTGCCA
    TACCTGTTCC ACGTCGTCAC CCTGCTGGTG GCCACGGGCC CTCTCTCGCT GCGTGCATCA
    ACACACGGCC TGGTCATCAA CATCATTCAC TCGCTCTGCA CATGCTCACA ACTCAACTTC
    AGTGAGGAGA CTAAACAGGT GCTGCGCCTG AGTCTGACTG AGTTCTCCCT GCCCAAGTTC
    TACCTGCTGT TCGGCATCAG TAAGGTCAAA TCTGCGGCGG TCATCGCTTT CCGTTCCAGC
    TACAGAGACC GCTCATTCTC TCCAGGCTCT TATGAGAGAG AGACATTTGC TCTGTCTTCC
    CTGGAAACAG TCACGGAGGC TCTTTTGGAG ATCATGGAGG CCTGCATGAG GGACATTCCT
    GGATGCAAAT GGCTAGATCA GTGGACTGAA TTGGCGCAAA AGTTTGCGTT CCAGTATAAC
    CCCTCTCTTC AGCCTCGGGC GCTGGTGGTG TTCGGCTGCA TCAGTAAACG AGTGACTCAC
    GGACAGCTAA AGCAGATCAT TCGTATTCTC AGTAAAGCCA GTCCTCTACT CAGCATTGAT
    CGGGGGCTGG AGAGCTGCTT AAAGGGCCCA GATAATTACA ACAGCCAGGT TCTCATCGAA
    GCCACAGTCA TCGCTCTCAC CAAACTACAG CCTCTCCTCG ATCAGGATTC TCCTATGCAT
    AAAGCTCTGT TCTGGGTGGC CGTGGCCGTG CTTCAGTTGG ATGAAGTGAA TCTGTACTCC
    GCCGGCACTG CCTTACTGGA GCAGAACCTG CACACACTGG ACAGCCTCCG AGTGTTTAAT
    GATAAGAGTC CTGAAGAGGT TTTTATGGAG ATCCGGAAAC CTCTAGAGTG GCACTGTAAA
    CAAATGGACC ATTTTGTGGG ACTCAACTTT GCCTCCAACT TCAATTTTGC ATTAGTGGGC
    CATTTACTTA AAGGATACAG ACATCCCTCT TCAACAACAG TTGCCAGGAC AGTTCGTATC
    CTGCATACAC TCCTGGGCCT GGTGGGAAAA CACCGCAGCT GTGACAAGTT TGAGGTGAAC
    ACGCAGAGTG TGGCCTATCT GGCAGCTTTG CTCACAGTGT CAGAGGAAGT TAGGAGCCGC
    TGCAGTTTAA AGCACAGGAA ATCATTGCTG CTTTCAGAAG TTACAATGGA GAACTTCCCC
    ATGGACACAT ACACCGTTTA CGACACTGAA CCCGGGTGCA GGCTACTGCG TGAAAACCAG
    CCTTGGGCTT CTCCGAAAGT GTCAGAGCGC AGTCTTGCAC CTCCGTACTC CACAATAGGG
    CAGACCAGCC TTCGTCCACG CAAATCCATG AGTGTAGACA TGGGCCAGCC GTCTCAGGCC
    AATGCGAAGA AACTACTGGG TATTAGCGGC ACTAGAAAGA GTTTCGATCA CTTAATATCA
    GAATCCAAAG CCCCAAAAAG ACCAGACATG GAGTCGGGCA TCACCACACC TCCAAAGATG
    AGGCGGGTTT CTGAAAATGA CTATGAGATC GAAACTCAGC GAATAGCCAA CTCTCATTTA
    CGCAAAGTGT CTGTGTCTGA ATCCAATGTC CTCCTGGACG AGAACGTACT TACGGACCCC
    AAGATCCAGA CGTTGCTCCT CACTGTACTG GCAACACTGG TGAAGTACAC TACAGACGAG
    TTTCATCAGC GGATCTTGTA TGAGTATTTG GCTGAGGCCA GTGTGGTCTT CCCCAAGGTC
    TTTCCAGTTG TGCACAACCT GCTGGACTCC AAGTTAAGCA CCGTCCTTTC CATGTGCCAG
    GACCCGAACC TTCTGAACCC CGTCCATGGC ATCATTCAGA ACGTGGTCTA TCATGAGGAG
    TCTCCCCCTC AGTACCAGCC GTCCTGCTTG CAGAGCTTTG GTTTCAATGG TCTTTGGAGG
    TTTGCCGGGC CGTTCTCTAA GCAAACCCAA ATTCCAGACT ACGCCGAGCT GGTGGTTAAA
    TTTTTGGATG CTTTGATTGA CACGTATCTC CCGGGCATTG AGGAGGAAGG AGGAGAAGAG
    TCACTGCGGA CCCCGACCTC GCCATATCCA CCAACCGTCC AGAGCCAGCT GAGCATCACA
    GCCAATCTCA ACCTGTCCAA CTCCATGACA TCACTTGCCA CATCACAGCA CTCCCCAGAG
    GGCCCTATAG TTTATAGACG GTCCCCAACT CTCTCCCATC CACGAACTAC AGAGGAGGTG
    CCACCGAAAA CAGCTGCCTG TCTGCCTTGC TCTAAGTCGG CTGTATTCCC ACCTCACCTC
    GTCCTGCCAG GCATCGACAA GGAGAACGTT GAGATTTCTC CCACCTCGAG TCTGTCCACC
    AGTGGGCGAA TGCGGCACGG CTCAGCAAGC CAGGTGCAGA AGCAGCGCAG CGCAGGCAGC
    TTCAAACGGC CAAGCATCAA AAAGATCGTC TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa39874
    Clone ID Related Accession (Same CDS sequence) XM_021466447.1
    Accession Version XM_021466447.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8421bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product neurofibromin isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    301
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGGCGGCGC ACAAGCCCGT GGAATGGGTC CAGGCCATCA TTAACAGATT CGACGAACAG 
    CTCCCAATCA AAACAGGACA GCAGAGCACC CACACCAAAG TGAGTACAGA GCACAACAAA
    GAATGTTTGA TCAACATCTC CAAGTACAAA TTTGCCCTCG TCATCAATGG CCTGACCAAC
    ATACTCAAAA ATGTGAACAA CATGCGGATA TTTGGCGAAG CTGCTGAGAA GAACCTCTAC
    TTCTCCCAGC TGATCATACT GGACACACTG GAGAAGTGTC TAGCAGGGCA ACCTAAGGAT
    TGCCTGAGGC TGGATGAGGC TATGTTGGTA AAACAGTTGC TGCCTGAAAT CTGTCACTTC
    CTGCACTCGT ACCGTGAGGG CCAGCAACAC GCCACTCAGC TGCGCAGCTC CGCCTCTGCT
    GTGCTTTTCT CTCTCAGCTG CAACAACTTT AATGCCGTCT TCAGTCGTAT CTCCACCAGG
    TTGCAAGAGC TCACAGTTTG TACAGAGGAC ACAGTGGATG TCCATGACAT TGAGCTCATT
    CAGTATATCA ATGTAGATTG CTCCAAGTTA AAGAGACTCC TACAAGAAGC AGTACTTAAG
    TTCAAGTCTT TGAAAAAGCC GGCTCAGCTT GCAGTAATCA GCAGTTTGGA AAAGGCTTTC
    TGGAACTGGG TTGAACACTA CCCTGATGAT TTCACTATGC TCTATCAGAG ACCCCAGGCC
    GACATGGCCG ACTGTGCAGA GAGACTGTTC GACCTAGTGG ACAGTTTTGC GGAAAGCGCA
    AAGAGAAAAG CTGCTGTGTG GCCACTGCAG ATCATCCTCC TCATCCTCTG TCCAGAGATC
    ACGCAGAGCA TCACCAAAGA AGTAGGGGGA GAGGAGAAAG CTAACAAGAA GTCATTTGTA
    GACAGTTTAC GAAAGGCTCT TGGCCAACAC AGCGGCAGCA AACAATTGAC GGAAAGTGCA
    GCTGTTGCAT GCGTCAAACT CTGCAAAGCC TGCACATATA TCAACTGGGA GGATAATTCT
    GTCATCTTTC TCCTGGTTCA GTCCATCGTC ATGGACCTTA AGGGTCTCCT GTTTAACCCC
    ACCAAACCTT TCTCAAGAGG TACGGGCTGC CAGAGCGCCG ATGTAGATTT GATGATCGAC
    TGCTTTGTGT CATGTTATCG CATCAATCCT CGCAACAACC AGCATTTTAA GGTGTGTCTG
    GCCTCGACTT CTCCTCCTAC CTTCCATTTC GTCCTTGTCA ACTCCCTGCA CAAAATTATC
    ACAAATTCTC ACCTGGACTG GTGGCCGAAG ATTGACGCCA TCTACTGCTA CTCAGGAGAA
    CTACGCACCA TGTTTGCAGA CACACTGGGT CGAGTGATTC AGAGTCTCAG TGCACATCCG
    CCTCTGAGGA TGACATCAAG TTTGACATTC AAAGAGAAAA TGACAACAAG TTTGAAGTTC
    AAGGAAAAGT CCACAGATCT TGATTCGCGT TCTTTGCTGC TGGCACTGGT CAAACTTATC
    CACGCTGACC CCAAACTAAT GCTGCACAAT CCGGGAAAAC CTGGTCAGGA GATTCAGAAC
    AGCACAGCTG AGATCATCAA AGGTCTGGTT CAGCTGGTTC CTCTTACCCA CAGTGCTGAG
    CTTTCCCAAG AGGCCATGGA GGCATTGCTT GTTTTACATC AGCCAGAAAC CATTGAGCTA
    TGGAACCCGG ACGCTCCCAT TGAGACCTTC TGGGACATCA GCTCTCAGGT GTTATTTCTA
    ATTTGTAAGA AGCTGATTGG ACACCAGATG ATCAACAGTA CAGAGATACT CAAGTGGCTC
    AGGGAGATTC TCATATGCAG GAACAAGTTT CTATTAAAGA ACAAGGAGTG TGCTACACAG
    GGCAGTCTGA TCCCCATCAG CAGACAGGCT CAGACCAAGC TGGAGGTGTG TCTGTACACG
    TTCCTGTGGA GTCCTGATGC AGAGGCTGTT TTGGTGGCCA TGTCCTGTTT CAGGCATCTC
    TGTGAGGAGG CCGATATTCG CTGCAGTGTC GATGAGCTGC CTGTTCACAC AGTCCTACCC
    AATTACAACA CCTTCATTGA GCTTGCATCC GTCAGCAATA TGATGGCCAC AGGTCGCAAC
    ACACTACAAA AAAGAGTGAT GGCACTTTTA AGAAGAATAA AGCACCCTAC AGCTGGAAAT
    GTAGAGGCAT GGGAAGACAC ATATGCAAAG TGGGAGCAAG CTACAAAACA GATACTCAAC
    CACCCAAAAA ACAAAGTGGA AGATGGACAG GAGTGGATCA ACATGACTGG TTTCTTGTGC
    GCTCTGGGAG GTGTTTGTCT CCAACAGCGC AGCACGCCGA GTCTGGGCAC CTACAGTCCT
    CCAATGGGCC CCCTGACCGA ACGCAAGGGG TCCATGATTT CCATGAGCTC CTGCGAGGTA
    AACAGCGAAA CATCCCTGAA TCGCTTTCTG GAGCGTCTGC TGGCTCTGAT GGTCTGCACA
    CATGAGAAAG TGGGAGTGCA AATTCGCACC AACGTCAAAG ACCTGGTGGG ACTGGAGCTC
    AGTCCGGCTC TCTATTCAAT GCTCTTCACT AAGCTGCGCA ACAGCATCGG ACGCTTCTTT
    GACACCCAGG GGCCGGTTCC TATTACAGAT GCAAACACTC TGTTTGTAGA GCAGACCATT
    GCTATCATGA AGAACTTGCT GGACAGTCAC ACTGAAGGAA GCTCAGAGCA CCTTGGTCAA
    GCCAGCATTG AGACCATGAT GCTCAATCTA GTTAGGTATG TGCGTATTTT AGGGAACATT
    GTTCATGCCA TTCAGATCAA AACCAAGTTG TGTCAGTTAG TGGAGGTGAT GATGGAGCGC
    AGGGATGACC TCTCCTTCTG CCAGGAGATG AAATTCAGGA ATAAAATGGT GGAGTACCTC
    ACAGACTGGG TGATGGGAAC CTCCAATCAA GCTGCGGACG ATGATGTCAA ATGCCTGACC
    CGCGATCTGG ACCAGGCCAG TATGGAGGCT GTCGTGTCTC TGTTGGCTGG TCTTCCTCTA
    CAGCCTGAGG AGGGTGACGG TGTTGAGCTC ATGGAGGCCA AATCACAGCT CTTCCTGAAG
    TACTTCACAC TGTTCATGAA CCTGTTGAAT GACTGCAGCG AGGTTGAGGA AGATGGCCAG
    CAGGTCGCAG GGAGGAAACG TGGCATGTCT AGACGACTGG CATCTCTGCG GCACTGCACA
    GTATTGGCTA TGTCTAACTT GCTCAATGCT AATGTGGATA GCGGCCTCAT GCACTCAATC
    GGTCTGGGAT ACCATAAGGA CCTGCAGACA CGGGCTACAT TTATGGAAGT GCTCACTAAG
    ATCCTTCAGC AAGGGACGGA GTTCGACACG CTTGCAGAGA CTGTGCTTGC AGATCGCTTT
    GAGAGACTGG TCGAGCTGGT CACCATGATG GGAGATCAGG GAGAGCTGCC CATAGCCATG
    GCTCTGGCCA ATGTGGTCCC ATGCTCTCAA TGGGATGAGC TGGCCAGAGT GTTAGTGACT
    CTCTTCGACT CTCGCCATCT GCTGTATCAG CTGTTGTGGA ATATGTTCTC CAAAGAGGTG
    GAGCTGGCAG ACTCAATGCA GACGCTCTTT AGAGGAAATA GCCTAGCCAG CAAGATTATG
    ACCTTCTGCT TCAAGGTGTA CGGAGCTGCG TATCTACAGA AGCTTCTGGA GCCGCTGTTG
    AGAGGAATCA TCACTACACC AGAGTGGCAG CAGATCAGTT TTGAAGTGGA CCCTACTAGA
    CTTGAACAGG CAGAGAACCT GGAGGAGAAC CAGAAGAACC TGCTTCAGAT CACTGACCGC
    TTCTTCCTGG CCATCATCAA CTCCTCGAGC GAGTTTCCTC CACAGCTGCG CAGTGTGTGC
    CACTGTCTGT ATCAGGCTAC TTGCCACTCC CTTCTGAATA AAGCCACTGT TAAAGAAAAA
    AAGGAAAACA AAAAATCCGT GGTCAGTCAG CGGTTTCCTC AGAACAGCAT TGGTGCTGTA
    GGAAGTGCCA TGTTCCTGCG CTTTATTAAC CCAGCTATCG TGTCCCCATA TGAGGCTGGA
    ATCCTAGACA AGAAACCCCC ACCTCGCATC GAGCGTGGCC TTAAACTCAT GTCTAAGATT
    CTGCAGAGCA TTGCTAATCA TGTTCTCTTC ACAAAAGAGG AGCATATGAG GCCCTTTAAT
    GATTTTGTCA AAAGCAACTT CGATGCAGCC AGAAGGTTCT TCTTGGATAT CGCATCTGAT
    TCTCCACCAA GTGATTCTGT GAACCATAGT CTGTCTTTCA TCAGTGACGG TAATGTGCTC
    GCTCTGCACC GGCTATTATG GAACAACCAG GAAAGAATTG GCCAATACCT GTCCAGCAAC
    AGGGACCACA AGGCAGTGGG CCGTAGACCA TTTGACAAGA TGGCCACTCT TCTGGCGTAC
    CTGGGTCCTC CTGAACATAA GCCAGTAGCA GACACACACT GGTCCAGCCT CAACCTCACC
    AGCTCAAAGT TTGAGGAGTT CATGACAAGG CATCAAGTCC ATGAGAAGGA GGAATTTAAA
    GCATTAAAAA CCCTCAACAT ATTTTACCAA GCTGGCACAT CTAAGAACGG CAACCCTGTG
    TTTTATTACA TCGCACGCAG GTTCAAAACT GGACAAATCA ATGGTGATCT TCTGATCTAC
    CACGTCCTGC TGACGCTCAA GCCCTACTAT GCCAAGCATT ATGAGATCGT AGTTGACCTT
    ACTCATGTGG GCCCCAGTAA CCGTTTCAAG ACGGACTTCT TATCCAAATG GTTTGTGGTC
    TTTCCAAACT TCGCCTATGA GAACGTGGCT GCTGTGTACA TCTACAACTG CAACACCTGG
    GTACGCGAGT ACACCAAATA CCACGAGCGA CTGCTGACTG GCCTCAAGGG GAGCAAGAAG
    CTGGTGTTTA TCGATTCGCC TGCACGTCTA GCTGAACACA TCGAACCCGA ACAGCAGAAG
    CTGCCCGCAG CTACTTTAGC ACTGGAAGAA GACCTCAAAG TGTTCCACAA CGCCCTAAAG
    CTTGCACACA GAGACACAAA AGTGTCAATT AAGGTGGGAT CCACAGCTGT ACAGGTGACC
    TCGGCCGAAA GAACGCGTGT CCTAGGACAG CCTGTGTTCC TAAATGACAT CTACTACGCT
    TCAGAGATCG AGGAGATATG CCTGGTGGAC GAGAACCAGT TCACGCTAAC CATCGCTAAC
    CAGGGCACCC CACTCACTTT CATGCACCAG GAGTGTGAAG CCATCGTTCA GTCCATCATC
    CACATCCGCA CGCGCTGGGA ACTTTCCCAA CCAGACTCCA TTCCCCAACA CACCAAAATC
    AGGCCTAAGG ATGTTCCTGG CACCCTGCTC AACATCGCTC TTCTCAACCT GGGAAGCTCC
    GACCCCAGTC TGAGGTCTGC GGCCTACAAT CTCCTTTGTG CCTTAACCTG CACCTTCAAC
    CTGAAGATTG AAGGCCAGCT TCTGGAAACG TCTGGCTTGT GCATCCCAGC CAACAACACG
    CTTTTTATTG TGTCCATTAG CAAAACGCTA GCAGCTAATG AACCCCACCT CACTCTGGAG
    TTCCTGGAAG AGTGCATCTC AGGATTCAGT AAATCAAGTA TAGAGCTGAA GCATCTGTGT
    CTAGAATATA TGACTCCTTG GCTCCGTAAT TTGGTGCGCT TTTGCAAACA CAATGATGAC
    GCCAAACGGC AGCGTGTCAC GGCCATCCTG GACAAGCTTA TTACTATGAC CATCAACGAG
    AAGCAGATGT ACCCCTCCAT ACAGGCCAAG ATATGGGGCA GCTTGGGTCA GATCACAGAC
    CTGCTTGATG TGGTGTTGGA CAGCTTTATA AAGACCAGCG CTACTGGCGG TTTAGGATCC
    ATCAAAGCTG AAGTGATGGC TGACACTGCT GTAGCTTTGG CGTCAGGAAA TGTCAAACTG
    GTCTCCAGCA AGGTGATTGG CCGAATGTGT AAGATCATCG ACAAGACGTG TCTGTCTCCC
    ACTCCGACCC TTGAGCAGCA CCTCATGTGG GACGACATCG CCATCCTGGC CCGGTACATG
    CTCATGCTGT CCTTCAACAA CTCTCTGGAC GTGGCCGCTC ATCTGCCATA CCTGTTCCAC
    GTCGTCACCC TGCTGGTGGC CACGGGCCCT CTCTCGCTGC GTGCATCAAC ACACGGCCTG
    GTCATCAACA TCATTCACTC GCTCTGCACA TGCTCACAAC TCAACTTCAG TGAGGAGACT
    AAACAGGTGC TGCGCCTGAG TCTGACTGAG TTCTCCCTGC CCAAGTTCTA CCTGCTGTTC
    GGCATCAGTA AGGTCAAATC TGCGGCGGTC ATCGCTTTCC GTTCCAGCTA CAGAGACCGC
    TCATTCTCTC CAGGCTCTTA TGAGAGAGAG ACATTTGCTC TGTCTTCCCT GGAAACAGTC
    ACGGAGGCTC TTTTGGAGAT CATGGAGGCC TGCATGAGGG ACATTCCTGG ATGCAAATGG
    CTAGATCAGT GGACTGAATT GGCGCAAAAG TTTGCGTTCC AGTATAACCC CTCTCTTCAG
    CCTCGGGCGC TGGTGGTGTT CGGCTGCATC AGTAAACGAG TGACTCACGG ACAGCTAAAG
    CAGATCATTC GTATTCTCAG TAAAGCCAGT CCTCTACTCA GCATTGATCG GGGGCTGGAG
    AGCTGCTTAA AGGGCCCAGA TAATTACAAC AGCCAGGTTC TCATCGAAGC CACAGTCATC
    GCTCTCACCA AACTACAGCC TCTCCTCGAT CAGGATTCTC CTATGCATAA AGCTCTGTTC
    TGGGTGGCCG TGGCCGTGCT TCAGTTGGAT GAAGTGAATC TGTACTCCGC CGGCACTGCC
    TTACTGGAGC AGAACCTGCA CACACTGGAC AGCCTCCGAG TGTTTAATGA TAAGAGTCCT
    GAAGAGGTTT TTATGGAGAT CCGGAAACCT CTAGAGTGGC ACTGTAAACA AATGGACCAT
    TTTGTGGGAC TCAACTTTGC CTCCAACTTC AATTTTGCAT TAGTGGGCCA TTTACTTAAA
    GGATACAGAC ATCCCTCTTC AACAACAGTT GCCAGGACAG TTCGTATCCT GCATACACTC
    CTGGGCCTGG TGGGAAAACA CCGCAGCTGT GACAAGTTTG AGGTGAACAC GCAGAGTGTG
    GCCTATCTGG CAGCTTTGCT CACAGTGTCA GAGGAAGTTA GGAGCCGCTG CAGTTTAAAG
    CACAGGAAAT CATTGCTGCT TTCAGAAGTT ACAATGGAGA ACTTCCCCAT GGACACATAC
    ACCGTTTACG ACACTGAACC CGGGTGCAGG CTACTGCGTG AAAACCAGCC TTGGGCTTCT
    CCGAAAGTGT CAGAGCGCAG TCTTGCACCT CCGTACTCCA CAATAGGGCA GACCAGCCTT
    CGTCCACGCA AATCCATGAG TGTAGACATG GGCCAGCCGT CTCAGGCCAA TGCGAAGAAA
    CTACTGGGTA TTAGCGGCAC TAGAAAGAGT TTCGATCACT TAATATCAGA ATCCAAAGCC
    CCAAAAAGAC CAGACATGGA GTCGGGCATC ACCACACCTC CAAAGATGAG GCGGGTTTCT
    GAAAATGACT ATGAGATCGA AACTCAGCGA ATAGCCAACT CTCATTTACG CAAAGTGTCT
    GTGTCTGAAT CCAATGTCCT CCTGGACGAG AACGTACTTA CGGACCCCAA GATCCAGACG
    TTGCTCCTCA CTGTACTGGC AACACTGGTG AAGTACACTA CAGACGAGTT TCATCAGCGG
    ATCTTGTATG AGTATTTGGC TGAGGCCAGT GTGGTCTTCC CCAAGGTCTT TCCAGTTGTG
    CACAACCTGC TGGACTCCAA GTTAAGCACC GTCCTTTCCA TGTGCCAGGA CCCGAACCTT
    CTGAACCCCG TCCATGGCAT CATTCAGAAC GTGGTCTATC ATGAGGAGTC TCCCCCTCAG
    TACCAGCCGT CCTGCTTGCA GAGCTTTGGT TTCAATGGTC TTTGGAGGTT TGCCGGGCCG
    TTCTCTAAGC AAACCCAAAT TCCAGACTAC GCCGAGCTGG TGGTTAAATT TTTGGATGCT
    TTGATTGACA CGTATCTCCC GGGCATTGAG GAGGAAGGAG GAGAAGAGTC ACTGCGGACC
    CCGACCTCGC CATATCCACC AACCGTCCAG AGCCAGCTGA GCATCACAGC CAATCTCAAC
    CTGTCCAACT CCATGACATC ACTTGCCACA TCACAGCACT CCCCAGAGGG CCCTATAGTT
    TATAGACGGT CCCCAACTCT CTCCCATCCA CGAACTACAG AGGAGGTGCC ACCGAAAACA
    GCTGCCTGTC TGCCTTGCTC TAAGTCGGCT GTATTCCCAC CTCACCTCGT CCTGCCAGGC
    ATCGACAAGG AGAACGTTGA GATTTCTCCC ACCTCGAGTC TGTCCACCAG TGGGCGAATG
    CGGCACGGCT CAGCAAGCCA GGTGCAGAAG CAGCGCAGCG CAGGCAGCTT CAAACGGCCA
    AGCATCAAAA AGATCGTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322122.1
    CDS247..8667
    Translation

    Target ORF information:

    RefSeq Version XM_021466447.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio neurofibromin 1a (nf1a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466447.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGGCGGCGC ACAAGCCCGT GGAATGGGTC CAGGCCATCA TTAACAGATT CGACGAACAG 
    CTCCCAATCA AAACAGGACA GCAGAGCACC CACACCAAAG TGAGTACAGA GCACAACAAA
    GAATGTTTGA TCAACATCTC CAAGTACAAA TTTGCCCTCG TCATCAATGG CCTGACCAAC
    ATACTCAAAA ATGTGAACAA CATGCGGATA TTTGGCGAAG CTGCTGAGAA GAACCTCTAC
    TTCTCCCAGC TGATCATACT GGACACACTG GAGAAGTGTC TAGCAGGGCA ACCTAAGGAT
    TGCCTGAGGC TGGATGAGGC TATGTTGGTA AAACAGTTGC TGCCTGAAAT CTGTCACTTC
    CTGCACTCGT ACCGTGAGGG CCAGCAACAC GCCACTCAGC TGCGCAGCTC CGCCTCTGCT
    GTGCTTTTCT CTCTCAGCTG CAACAACTTT AATGCCGTCT TCAGTCGTAT CTCCACCAGG
    TTGCAAGAGC TCACAGTTTG TACAGAGGAC ACAGTGGATG TCCATGACAT TGAGCTCATT
    CAGTATATCA ATGTAGATTG CTCCAAGTTA AAGAGACTCC TACAAGAAGC AGTACTTAAG
    TTCAAGTCTT TGAAAAAGCC GGCTCAGCTT GCAGTAATCA GCAGTTTGGA AAAGGCTTTC
    TGGAACTGGG TTGAACACTA CCCTGATGAT TTCACTATGC TCTATCAGAG ACCCCAGGCC
    GACATGGCCG ACTGTGCAGA GAGACTGTTC GACCTAGTGG ACAGTTTTGC GGAAAGCGCA
    AAGAGAAAAG CTGCTGTGTG GCCACTGCAG ATCATCCTCC TCATCCTCTG TCCAGAGATC
    ACGCAGAGCA TCACCAAAGA AGTAGGGGGA GAGGAGAAAG CTAACAAGAA GTCATTTGTA
    GACAGTTTAC GAAAGGCTCT TGGCCAACAC AGCGGCAGCA AACAATTGAC GGAAAGTGCA
    GCTGTTGCAT GCGTCAAACT CTGCAAAGCC TGCACATATA TCAACTGGGA GGATAATTCT
    GTCATCTTTC TCCTGGTTCA GTCCATCGTC ATGGACCTTA AGGGTCTCCT GTTTAACCCC
    ACCAAACCTT TCTCAAGAGG TACGGGCTGC CAGAGCGCCG ATGTAGATTT GATGATCGAC
    TGCTTTGTGT CATGTTATCG CATCAATCCT CGCAACAACC AGCATTTTAA GGTGTGTCTG
    GCCTCGACTT CTCCTCCTAC CTTCCATTTC GTCCTTGTCA ACTCCCTGCA CAAAATTATC
    ACAAATTCTC ACCTGGACTG GTGGCCGAAG ATTGACGCCA TCTACTGCTA CTCAGGAGAA
    CTACGCACCA TGTTTGCAGA CACACTGGGT CGAGTGATTC AGAGTCTCAG TGCACATCCG
    CCTCTGAGGA TGACATCAAG TTTGACATTC AAAGAGAAAA TGACAACAAG TTTGAAGTTC
    AAGGAAAAGT CCACAGATCT TGATTCGCGT TCTTTGCTGC TGGCACTGGT CAAACTTATC
    CACGCTGACC CCAAACTAAT GCTGCACAAT CCGGGAAAAC CTGGTCAGGA GATTCAGAAC
    AGCACAGCTG AGATCATCAA AGGTCTGGTT CAGCTGGTTC CTCTTACCCA CAGTGCTGAG
    CTTTCCCAAG AGGCCATGGA GGCATTGCTT GTTTTACATC AGCCAGAAAC CATTGAGCTA
    TGGAACCCGG ACGCTCCCAT TGAGACCTTC TGGGACATCA GCTCTCAGGT GTTATTTCTA
    ATTTGTAAGA AGCTGATTGG ACACCAGATG ATCAACAGTA CAGAGATACT CAAGTGGCTC
    AGGGAGATTC TCATATGCAG GAACAAGTTT CTATTAAAGA ACAAGGAGTG TGCTACACAG
    GGCAGTCTGA TCCCCATCAG CAGACAGGCT CAGACCAAGC TGGAGGTGTG TCTGTACACG
    TTCCTGTGGA GTCCTGATGC AGAGGCTGTT TTGGTGGCCA TGTCCTGTTT CAGGCATCTC
    TGTGAGGAGG CCGATATTCG CTGCAGTGTC GATGAGCTGC CTGTTCACAC AGTCCTACCC
    AATTACAACA CCTTCATTGA GCTTGCATCC GTCAGCAATA TGATGGCCAC AGGTCGCAAC
    ACACTACAAA AAAGAGTGAT GGCACTTTTA AGAAGAATAA AGCACCCTAC AGCTGGAAAT
    GTAGAGGCAT GGGAAGACAC ATATGCAAAG TGGGAGCAAG CTACAAAACA GATACTCAAC
    CACCCAAAAA ACAAAGTGGA AGATGGACAG GAGTGGATCA ACATGACTGG TTTCTTGTGC
    GCTCTGGGAG GTGTTTGTCT CCAACAGCGC AGCACGCCGA GTCTGGGCAC CTACAGTCCT
    CCAATGGGCC CCCTGACCGA ACGCAAGGGG TCCATGATTT CCATGAGCTC CTGCGAGGTA
    AACAGCGAAA CATCCCTGAA TCGCTTTCTG GAGCGTCTGC TGGCTCTGAT GGTCTGCACA
    CATGAGAAAG TGGGAGTGCA AATTCGCACC AACGTCAAAG ACCTGGTGGG ACTGGAGCTC
    AGTCCGGCTC TCTATTCAAT GCTCTTCACT AAGCTGCGCA ACAGCATCGG ACGCTTCTTT
    GACACCCAGG GGCCGGTTCC TATTACAGAT GCAAACACTC TGTTTGTAGA GCAGACCATT
    GCTATCATGA AGAACTTGCT GGACAGTCAC ACTGAAGGAA GCTCAGAGCA CCTTGGTCAA
    GCCAGCATTG AGACCATGAT GCTCAATCTA GTTAGGTATG TGCGTATTTT AGGGAACATT
    GTTCATGCCA TTCAGATCAA AACCAAGTTG TGTCAGTTAG TGGAGGTGAT GATGGAGCGC
    AGGGATGACC TCTCCTTCTG CCAGGAGATG AAATTCAGGA ATAAAATGGT GGAGTACCTC
    ACAGACTGGG TGATGGGAAC CTCCAATCAA GCTGCGGACG ATGATGTCAA ATGCCTGACC
    CGCGATCTGG ACCAGGCCAG TATGGAGGCT GTCGTGTCTC TGTTGGCTGG TCTTCCTCTA
    CAGCCTGAGG AGGGTGACGG TGTTGAGCTC ATGGAGGCCA AATCACAGCT CTTCCTGAAG
    TACTTCACAC TGTTCATGAA CCTGTTGAAT GACTGCAGCG AGGTTGAGGA AGATGGCCAG
    CAGGTCGCAG GGAGGAAACG TGGCATGTCT AGACGACTGG CATCTCTGCG GCACTGCACA
    GTATTGGCTA TGTCTAACTT GCTCAATGCT AATGTGGATA GCGGCCTCAT GCACTCAATC
    GGTCTGGGAT ACCATAAGGA CCTGCAGACA CGGGCTACAT TTATGGAAGT GCTCACTAAG
    ATCCTTCAGC AAGGGACGGA GTTCGACACG CTTGCAGAGA CTGTGCTTGC AGATCGCTTT
    GAGAGACTGG TCGAGCTGGT CACCATGATG GGAGATCAGG GAGAGCTGCC CATAGCCATG
    GCTCTGGCCA ATGTGGTCCC ATGCTCTCAA TGGGATGAGC TGGCCAGAGT GTTAGTGACT
    CTCTTCGACT CTCGCCATCT GCTGTATCAG CTGTTGTGGA ATATGTTCTC CAAAGAGGTG
    GAGCTGGCAG ACTCAATGCA GACGCTCTTT AGAGGAAATA GCCTAGCCAG CAAGATTATG
    ACCTTCTGCT TCAAGGTGTA CGGAGCTGCG TATCTACAGA AGCTTCTGGA GCCGCTGTTG
    AGAGGAATCA TCACTACACC AGAGTGGCAG CAGATCAGTT TTGAAGTGGA CCCTACTAGA
    CTTGAACAGG CAGAGAACCT GGAGGAGAAC CAGAAGAACC TGCTTCAGAT CACTGACCGC
    TTCTTCCTGG CCATCATCAA CTCCTCGAGC GAGTTTCCTC CACAGCTGCG CAGTGTGTGC
    CACTGTCTGT ATCAGGCTAC TTGCCACTCC CTTCTGAATA AAGCCACTGT TAAAGAAAAA
    AAGGAAAACA AAAAATCCGT GGTCAGTCAG CGGTTTCCTC AGAACAGCAT TGGTGCTGTA
    GGAAGTGCCA TGTTCCTGCG CTTTATTAAC CCAGCTATCG TGTCCCCATA TGAGGCTGGA
    ATCCTAGACA AGAAACCCCC ACCTCGCATC GAGCGTGGCC TTAAACTCAT GTCTAAGATT
    CTGCAGAGCA TTGCTAATCA TGTTCTCTTC ACAAAAGAGG AGCATATGAG GCCCTTTAAT
    GATTTTGTCA AAAGCAACTT CGATGCAGCC AGAAGGTTCT TCTTGGATAT CGCATCTGAT
    TCTCCACCAA GTGATTCTGT GAACCATAGT CTGTCTTTCA TCAGTGACGG TAATGTGCTC
    GCTCTGCACC GGCTATTATG GAACAACCAG GAAAGAATTG GCCAATACCT GTCCAGCAAC
    AGGGACCACA AGGCAGTGGG CCGTAGACCA TTTGACAAGA TGGCCACTCT TCTGGCGTAC
    CTGGGTCCTC CTGAACATAA GCCAGTAGCA GACACACACT GGTCCAGCCT CAACCTCACC
    AGCTCAAAGT TTGAGGAGTT CATGACAAGG CATCAAGTCC ATGAGAAGGA GGAATTTAAA
    GCATTAAAAA CCCTCAACAT ATTTTACCAA GCTGGCACAT CTAAGAACGG CAACCCTGTG
    TTTTATTACA TCGCACGCAG GTTCAAAACT GGACAAATCA ATGGTGATCT TCTGATCTAC
    CACGTCCTGC TGACGCTCAA GCCCTACTAT GCCAAGCATT ATGAGATCGT AGTTGACCTT
    ACTCATGTGG GCCCCAGTAA CCGTTTCAAG ACGGACTTCT TATCCAAATG GTTTGTGGTC
    TTTCCAAACT TCGCCTATGA GAACGTGGCT GCTGTGTACA TCTACAACTG CAACACCTGG
    GTACGCGAGT ACACCAAATA CCACGAGCGA CTGCTGACTG GCCTCAAGGG GAGCAAGAAG
    CTGGTGTTTA TCGATTCGCC TGCACGTCTA GCTGAACACA TCGAACCCGA ACAGCAGAAG
    CTGCCCGCAG CTACTTTAGC ACTGGAAGAA GACCTCAAAG TGTTCCACAA CGCCCTAAAG
    CTTGCACACA GAGACACAAA AGTGTCAATT AAGGTGGGAT CCACAGCTGT ACAGGTGACC
    TCGGCCGAAA GAACGCGTGT CCTAGGACAG CCTGTGTTCC TAAATGACAT CTACTACGCT
    TCAGAGATCG AGGAGATATG CCTGGTGGAC GAGAACCAGT TCACGCTAAC CATCGCTAAC
    CAGGGCACCC CACTCACTTT CATGCACCAG GAGTGTGAAG CCATCGTTCA GTCCATCATC
    CACATCCGCA CGCGCTGGGA ACTTTCCCAA CCAGACTCCA TTCCCCAACA CACCAAAATC
    AGGCCTAAGG ATGTTCCTGG CACCCTGCTC AACATCGCTC TTCTCAACCT GGGAAGCTCC
    GACCCCAGTC TGAGGTCTGC GGCCTACAAT CTCCTTTGTG CCTTAACCTG CACCTTCAAC
    CTGAAGATTG AAGGCCAGCT TCTGGAAACG TCTGGCTTGT GCATCCCAGC CAACAACACG
    CTTTTTATTG TGTCCATTAG CAAAACGCTA GCAGCTAATG AACCCCACCT CACTCTGGAG
    TTCCTGGAAG AGTGCATCTC AGGATTCAGT AAATCAAGTA TAGAGCTGAA GCATCTGTGT
    CTAGAATATA TGACTCCTTG GCTCCGTAAT TTGGTGCGCT TTTGCAAACA CAATGATGAC
    GCCAAACGGC AGCGTGTCAC GGCCATCCTG GACAAGCTTA TTACTATGAC CATCAACGAG
    AAGCAGATGT ACCCCTCCAT ACAGGCCAAG ATATGGGGCA GCTTGGGTCA GATCACAGAC
    CTGCTTGATG TGGTGTTGGA CAGCTTTATA AAGACCAGCG CTACTGGCGG TTTAGGATCC
    ATCAAAGCTG AAGTGATGGC TGACACTGCT GTAGCTTTGG CGTCAGGAAA TGTCAAACTG
    GTCTCCAGCA AGGTGATTGG CCGAATGTGT AAGATCATCG ACAAGACGTG TCTGTCTCCC
    ACTCCGACCC TTGAGCAGCA CCTCATGTGG GACGACATCG CCATCCTGGC CCGGTACATG
    CTCATGCTGT CCTTCAACAA CTCTCTGGAC GTGGCCGCTC ATCTGCCATA CCTGTTCCAC
    GTCGTCACCC TGCTGGTGGC CACGGGCCCT CTCTCGCTGC GTGCATCAAC ACACGGCCTG
    GTCATCAACA TCATTCACTC GCTCTGCACA TGCTCACAAC TCAACTTCAG TGAGGAGACT
    AAACAGGTGC TGCGCCTGAG TCTGACTGAG TTCTCCCTGC CCAAGTTCTA CCTGCTGTTC
    GGCATCAGTA AGGTCAAATC TGCGGCGGTC ATCGCTTTCC GTTCCAGCTA CAGAGACCGC
    TCATTCTCTC CAGGCTCTTA TGAGAGAGAG ACATTTGCTC TGTCTTCCCT GGAAACAGTC
    ACGGAGGCTC TTTTGGAGAT CATGGAGGCC TGCATGAGGG ACATTCCTGG ATGCAAATGG
    CTAGATCAGT GGACTGAATT GGCGCAAAAG TTTGCGTTCC AGTATAACCC CTCTCTTCAG
    CCTCGGGCGC TGGTGGTGTT CGGCTGCATC AGTAAACGAG TGACTCACGG ACAGCTAAAG
    CAGATCATTC GTATTCTCAG TAAAGCCAGT CCTCTACTCA GCATTGATCG GGGGCTGGAG
    AGCTGCTTAA AGGGCCCAGA TAATTACAAC AGCCAGGTTC TCATCGAAGC CACAGTCATC
    GCTCTCACCA AACTACAGCC TCTCCTCGAT CAGGATTCTC CTATGCATAA AGCTCTGTTC
    TGGGTGGCCG TGGCCGTGCT TCAGTTGGAT GAAGTGAATC TGTACTCCGC CGGCACTGCC
    TTACTGGAGC AGAACCTGCA CACACTGGAC AGCCTCCGAG TGTTTAATGA TAAGAGTCCT
    GAAGAGGTTT TTATGGAGAT CCGGAAACCT CTAGAGTGGC ACTGTAAACA AATGGACCAT
    TTTGTGGGAC TCAACTTTGC CTCCAACTTC AATTTTGCAT TAGTGGGCCA TTTACTTAAA
    GGATACAGAC ATCCCTCTTC AACAACAGTT GCCAGGACAG TTCGTATCCT GCATACACTC
    CTGGGCCTGG TGGGAAAACA CCGCAGCTGT GACAAGTTTG AGGTGAACAC GCAGAGTGTG
    GCCTATCTGG CAGCTTTGCT CACAGTGTCA GAGGAAGTTA GGAGCCGCTG CAGTTTAAAG
    CACAGGAAAT CATTGCTGCT TTCAGAAGTT ACAATGGAGA ACTTCCCCAT GGACACATAC
    ACCGTTTACG ACACTGAACC CGGGTGCAGG CTACTGCGTG AAAACCAGCC TTGGGCTTCT
    CCGAAAGTGT CAGAGCGCAG TCTTGCACCT CCGTACTCCA CAATAGGGCA GACCAGCCTT
    CGTCCACGCA AATCCATGAG TGTAGACATG GGCCAGCCGT CTCAGGCCAA TGCGAAGAAA
    CTACTGGGTA TTAGCGGCAC TAGAAAGAGT TTCGATCACT TAATATCAGA ATCCAAAGCC
    CCAAAAAGAC CAGACATGGA GTCGGGCATC ACCACACCTC CAAAGATGAG GCGGGTTTCT
    GAAAATGACT ATGAGATCGA AACTCAGCGA ATAGCCAACT CTCATTTACG CAAAGTGTCT
    GTGTCTGAAT CCAATGTCCT CCTGGACGAG AACGTACTTA CGGACCCCAA GATCCAGACG
    TTGCTCCTCA CTGTACTGGC AACACTGGTG AAGTACACTA CAGACGAGTT TCATCAGCGG
    ATCTTGTATG AGTATTTGGC TGAGGCCAGT GTGGTCTTCC CCAAGGTCTT TCCAGTTGTG
    CACAACCTGC TGGACTCCAA GTTAAGCACC GTCCTTTCCA TGTGCCAGGA CCCGAACCTT
    CTGAACCCCG TCCATGGCAT CATTCAGAAC GTGGTCTATC ATGAGGAGTC TCCCCCTCAG
    TACCAGCCGT CCTGCTTGCA GAGCTTTGGT TTCAATGGTC TTTGGAGGTT TGCCGGGCCG
    TTCTCTAAGC AAACCCAAAT TCCAGACTAC GCCGAGCTGG TGGTTAAATT TTTGGATGCT
    TTGATTGACA CGTATCTCCC GGGCATTGAG GAGGAAGGAG GAGAAGAGTC ACTGCGGACC
    CCGACCTCGC CATATCCACC AACCGTCCAG AGCCAGCTGA GCATCACAGC CAATCTCAAC
    CTGTCCAACT CCATGACATC ACTTGCCACA TCACAGCACT CCCCAGAGGG CCCTATAGTT
    TATAGACGGT CCCCAACTCT CTCCCATCCA CGAACTACAG AGGAGGTGCC ACCGAAAACA
    GCTGCCTGTC TGCCTTGCTC TAAGTCGGCT GTATTCCCAC CTCACCTCGT CCTGCCAGGC
    ATCGACAAGG AGAACGTTGA GATTTCTCCC ACCTCGAGTC TGTCCACCAG TGGGCGAATG
    CGGCACGGCT CAGCAAGCCA GGTGCAGAAG CAGCGCAGCG CAGGCAGCTT CAAACGGCCA
    AGCATCAAAA AGATCGTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa39875
    Clone ID Related Accession (Same CDS sequence) XM_021466448.1
    Accession Version XM_021466448.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8418bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product neurofibromin isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGGCGGCGC ACAAGCCCGT GGAATGGGTC CAGGCCATCA TTAACAGATT CGACGAACAG 
    CTCCCAATCA AAACAGGACA GCAGAGCACC CACACCAAAG TGAGTACAGA GCACAACAAA
    GAATGTTTGA TCAACATCTC CAAGTACAAA TTTGCCCTCG TCATCAATGG CCTGACCAAC
    ATACTCAAAA ATGTGAACAA CATGCGGATA TTTGGCGAAG CTGCTGAGAA GAACCTCTAC
    TTCTCCCAGC TGATCATACT GGACACACTG GAGAAGTGTC TAGCAGGGCA ACCTAAGGAT
    TGCCTGAGGC TGGATGAGGC TATGTTGGTA AAACAGTTGC TGCCTGAAAT CTGTCACTTC
    CTGCACTCGT ACCGTGAGGG CCAGCAACAC GCCACTCAGC TGCGCAGCTC CGCCTCTGCT
    GTGCTTTTCT CTCTCAGCTG CAACAACTTT AATGCCGTCT TCAGTCGTAT CTCCACCAGG
    TTGCAAGAGC TCACAGTTTG TACAGAGGAC ACAGTGGATG TCCATGACAT TGAGCTCATT
    CAGTATATCA ATGTAGATTG CTCCAAGTTA AAGAGACTCC TACAAGAAGC AGTACTTAAG
    TTCAAGTCTT TGAAAAAGCC GGCTCAGCTT GCAGTAATCA GCAGTTTGGA AAAGGCTTTC
    TGGAACTGGG TTGAACACTA CCCTGATGAT TTCACTATGC TCTATCAGAG ACCCCAGGCC
    GACATGGCCG ACTGTGCAGA GAGACTGTTC GACCTAGTGG ACAGTTTTGC GGAAAGCGCA
    AAGAGAAAAG CTGCTGTGTG GCCACTGCAG ATCATCCTCC TCATCCTCTG TCCAGAGATC
    ACGCAGAGCA TCACCAAAGA AGTAGGGGGA GAGGAGAAAG CTAACAAGAA GTCATTTGTA
    GACAGTTTAC GAAAGGCTCT TGGCCAACAC AGCGGCAGCA AACAATTGAC GGAAAGTGCA
    GCTGTTGCAT GCGTCAAACT CTGCAAAGCC TGCACATATA TCAACTGGGA GGATAATTCT
    GTCATCTTTC TCCTGGTTCA GTCCATCGTC ATGGACCTTA AGGGTCTCCT GTTTAACCCC
    ACCAAACCTT TCTCAAGAGG TACGGGCTGC CAGAGCGCCG ATGTAGATTT GATGATCGAC
    TGCTTTGTGT CATGTTATCG CATCAATCCT CGCAACAACC AGCATTTTAA GGTGTGTCTG
    GCCTCGACTT CTCCTCCTAC CTTCCATTTC GTCCTTGTCA ACTCCCTGCA CAAAATTATC
    ACAAATTCTC ACCTGGACTG GTGGCCGAAG ATTGACGCCA TCTACTGCTA CTCAGGAGAA
    CTACGCACCA TGTTTGCAGA CACACTGGGT CGAGTGATTC AGAGTCTCAG TGCACATCCG
    CCTCTGAGGA TGACATCAAG TTTGACATTC AAAGAGAAAA TGACAACAAG TTTGAAGTTC
    AAGGAAAAGT CCACAGATCT TGATTCGCGT TCTTTGCTGC TGGCACTGGT CAAACTTATC
    CACGCTGACC CCAAACTAAT GCTGCACAAT CCGGGAAAAC CTGGTCAGGA GATTCAGAAC
    AGCACAGCTG AGATCATCAA AGGTCTGGTT CAGCTGGTTC CTCTTACCCA CAGTGCTGAG
    CTTTCCCAAG AGGCCATGGA GGCATTGCTT GTTTTACATC AGCCAGAAAC CATTGAGCTA
    TGGAACCCGG ACGCTCCCAT TGAGACCTTC TGGGACATCA GCTCTCAGGT GTTATTTCTA
    ATTTGTAAGA AGCTGATTGG ACACCAGATG ATCAACAGTA CAGAGATACT CAAGTGGCTC
    AGGGAGATTC TCATATGCAG GAACAAGTTT CTATTAAAGA ACAAGGAGTG TGCTACACAG
    GGCAGTCTGA TCCCCATCAG CAGACAGGCT CAGACCAAGC TGGAGGTGTG TCTGTACACG
    TTCCTGTGGA GTCCTGATGC AGAGGCTGTT TTGGTGGCCA TGTCCTGTTT CAGGCATCTC
    TGTGAGGAGG CCGATATTCG CTGCAGTGTC GATGAGCTGC CTGTTCACAC AGTCCTACCC
    AATTACAACA CCTTCATTGA GCTTGCATCC GTCAGCAATA TGATGGCCAC AGGTCGCAAC
    ACACTACAAA AAAGAGTGAT GGCACTTTTA AGAAGAATAA AGCACCCTAC AGCTGGAAAT
    GTAGAGGCAT GGGAAGACAC ATATGCAAAG TGGGAGCAAG CTACAAAACA GATACTCAAC
    CACCCAAAAA ACAAAGTGGA AGATGGACAG GTAGTTAACA TAAGATTGGT CAATTATTTC
    ATCTTTTTTT CCATTCATGG TCATCTATTT TCTGTTCGTC CTCAGGAGTG GATCAACATG
    ACTGGTTTCT TGTGCGCTCT GGGAGGTGTT TGTCTCCAAC AGCGCAGCAC GCCGAGTCTG
    GGCACCTACA GTCCTCCAAT GGGCCCCCTG ACCGAACGCA AGGGGTCCAT GATTTCCATG
    AGCTCCTGCG AGGTAAACAG CGAAACATCC CTGAATCGCT TTCTGGAGCG TCTGCTGGCT
    CTGATGGTCT GCACACATGA GAAAGTGGGA GTGCAAATTC GCACCAACGT CAAAGACCTG
    GTGGGACTGG AGCTCAGTCC GGCTCTCTAT TCAATGCTCT TCACTAAGCT GCGCAACAGC
    ATCGGACGCT TCTTTGACAC CCAGGGGCCG GTTCCTATTA CAGATGCAAA CACTCTGTTT
    GTAGAGCAGA CCATTGCTAT CATGAAGAAC TTGCTGGACA GTCACACTGA AGGAAGCTCA
    GAGCACCTTG GTCAAGCCAG CATTGAGACC ATGATGCTCA ATCTAGTTAG GTATGTGCGT
    ATTTTAGGGA ACATTGTTCA TGCCATTCAG ATCAAAACCA AGTTGTGTCA GTTAGTGGAG
    GTGATGATGG AGCGCAGGGA TGACCTCTCC TTCTGCCAGG AGATGAAATT CAGGAATAAA
    ATGGTGGAGT ACCTCACAGA CTGGGTGATG GGAACCTCCA ATCAAGCTGC GGACGATGAT
    GTCAAATGCC TGACCCGCGA TCTGGACCAG GCCAGTATGG AGGCTGTCGT GTCTCTGTTG
    GCTGGTCTTC CTCTACAGCC TGAGGAGGGT GACGGTGTTG AGCTCATGGA GGCCAAATCA
    CAGCTCTTCC TGAAGTACTT CACACTGTTC ATGAACCTGT TGAATGACTG CAGCGAGGTT
    GAGGAAGATG GCCAGCAGGT CGCAGGGAGG AAACGTGGCA TGTCTAGACG ACTGGCATCT
    CTGCGGCACT GCACAGTATT GGCTATGTCT AACTTGCTCA ATGCTAATGT GGATAGCGGC
    CTCATGCACT CAATCGGTCT GGGATACCAT AAGGACCTGC AGACACGGGC TACATTTATG
    GAAGTGCTCA CTAAGATCCT TCAGCAAGGG ACGGAGTTCG ACACGCTTGC AGAGACTGTG
    CTTGCAGATC GCTTTGAGAG ACTGGTCGAG CTGGTCACCA TGATGGGAGA TCAGGGAGAG
    CTGCCCATAG CCATGGCTCT GGCCAATGTG GTCCCATGCT CTCAATGGGA TGAGCTGGCC
    AGAGTGTTAG TGACTCTCTT CGACTCTCGC CATCTGCTGT ATCAGCTGTT GTGGAATATG
    TTCTCCAAAG AGGTGGAGCT GGCAGACTCA ATGCAGACGC TCTTTAGAGG AAATAGCCTA
    GCCAGCAAGA TTATGACCTT CTGCTTCAAG GTGTACGGAG CTGCGTATCT ACAGAAGCTT
    CTGGAGCCGC TGTTGAGAGG AATCATCACT ACACCAGAGT GGCAGCAGAT CAGTTTTGAA
    GTGGACCCTA CTAGACTTGA ACAGGCAGAG AACCTGGAGG AGAACCAGAA GAACCTGCTT
    CAGATCACTG ACCGCTTCTT CCTGGCCATC ATCAACTCCT CGAGCGAGTT TCCTCCACAG
    CTGCGCAGTG TGTGCCACTG TCTGTATCAG GCTACTTGCC ACTCCCTTCT GAATAAAGCC
    ACTGTTAAAG AAAAAAAGGA AAACAAAAAA TCCGTGGTCA GTCAGCGGTT TCCTCAGAAC
    AGCATTGGTG CTGTAGGAAG TGCCATGTTC CTGCGCTTTA TTAACCCAGC TATCGTGTCC
    CCATATGAGG CTGGAATCCT AGACAAGAAA CCCCCACCTC GCATCGAGCG TGGCCTTAAA
    CTCATGTCTA AGATTCTGCA GAGCATTGCT AATCATGTTC TCTTCACAAA AGAGGAGCAT
    ATGAGGCCCT TTAATGATTT TGTCAAAAGC AACTTCGATG CAGCCAGAAG GTTCTTCTTG
    GATATCGCAT CTGATTCTCC ACCAAGTGAT TCTGTGAACC ATAGTCTGTC TTTCATCAGT
    GACGGTAATG TGCTCGCTCT GCACCGGCTA TTATGGAACA ACCAGGAAAG AATTGGCCAA
    TACCTGTCCA GCAACAGGGA CCACAAGGCA GTGGGCCGTA GACCATTTGA CAAGATGGCC
    ACTCTTCTGG CGTACCTGGG TCCTCCTGAA CATAAGCCAG TAGCAGACAC ACACTGGTCC
    AGCCTCAACC TCACCAGCTC AAAGTTTGAG GAGTTCATGA CAAGGCATCA AGTCCATGAG
    AAGGAGGAAT TTAAAGCATT AAAAACCCTC AACATATTTT ACCAAGCTGG CACATCTAAG
    AACGGCAACC CTGTGTTTTA TTACATCGCA CGCAGGTTCA AAACTGGACA AATCAATGGT
    GATCTTCTGA TCTACCACGT CCTGCTGACG CTCAAGCCCT ACTATGCCAA GCATTATGAG
    ATCGTAGTTG ACCTTACTCA TGTGGGCCCC AGTAACCGTT TCAAGACGGA CTTCTTATCC
    AAATGGTTTG TGGTCTTTCC AAACTTCGCC TATGAGAACG TGGCTGCTGT GTACATCTAC
    AACTGCAACA CCTGGGTACG CGAGTACACC AAATACCACG AGCGACTGCT GACTGGCCTC
    AAGGGGAGCA AGAAGCTGGT GTTTATCGAT TCGCCTGCAC GTCTAGCTGA ACACATCGAA
    CCCGAACAGC AGAAGCTGCC CGCAGCTACT TTAGCACTGG AAGAAGACCT CAAAGTGTTC
    CACAACGCCC TAAAGCTTGC ACACAGAGAC ACAAAAGTGT CAATTAAGGT GGGATCCACA
    GCTGTACAGG TGACCTCGGC CGAAAGAACG CGTGTCCTAG GACAGCCTGT GTTCCTAAAT
    GACATCTACT ACGCTTCAGA GATCGAGGAG ATATGCCTGG TGGACGAGAA CCAGTTCACG
    CTAACCATCG CTAACCAGGG CACCCCACTC ACTTTCATGC ACCAGGAGTG TGAAGCCATC
    GTTCAGTCCA TCATCCACAT CCGCACGCGC TGGGAACTTT CCCAACCAGA CTCCATTCCC
    CAACACACCA AAATCAGGCC TAAGGATGTT CCTGGCACCC TGCTCAACAT CGCTCTTCTC
    AACCTGGGAA GCTCCGACCC CAGTCTGAGG TCTGCGGCCT ACAATCTCCT TTGTGCCTTA
    ACCTGCACCT TCAACCTGAA GATTGAAGGC CAGCTTCTGG AAACGTCTGG CTTGTGCATC
    CCAGCCAACA ACACGCTTTT TATTGTGTCC ATTAGCAAAA CGCTAGCAGC TAATGAACCC
    CACCTCACTC TGGAGTTCCT GGAAGAGTGC ATCTCAGGAT TCAGTAAATC AAGTATAGAG
    CTGAAGCATC TGTGTCTAGA ATATATGACT CCTTGGCTCC GTAATTTGGT GCGCTTTTGC
    AAACACAATG ATGACGCCAA ACGGCAGCGT GTCACGGCCA TCCTGGACAA GCTTATTACT
    ATGACCATCA ACGAGAAGCA GATGTACCCC TCCATACAGG CCAAGATATG GGGCAGCTTG
    GGTCAGATCA CAGACCTGCT TGATGTGGTG TTGGACAGCT TTATAAAGAC CAGCGCTACT
    GGCGGTTTAG GATCCATCAA AGCTGAAGTG ATGGCTGACA CTGCTGTAGC TTTGGCGTCA
    GGAAATGTCA AACTGGTCTC CAGCAAGGTG ATTGGCCGAA TGTGTAAGAT CATCGACAAG
    ACGTGTCTGT CTCCCACTCC GACCCTTGAG CAGCACCTCA TGTGGGACGA CATCGCCATC
    CTGGCCCGGT ACATGCTCAT GCTGTCCTTC AACAACTCTC TGGACGTGGC CGCTCATCTG
    CCATACCTGT TCCACGTCGT CACCCTGCTG GTGGCCACGG GCCCTCTCTC GCTGCGTGCA
    TCAACACACG GCCTGGTCAT CAACATCATT CACTCGCTCT GCACATGCTC ACAACTCAAC
    TTCAGTGAGG AGACTAAACA GGTGCTGCGC CTGAGTCTGA CTGAGTTCTC CCTGCCCAAG
    TTCTACCTGC TGTTCGGCAT CAGTAAGGTC AAATCTGCGG CGGTCATCGC TTTCCGTTCC
    AGCTACAGAG ACCGCTCATT CTCTCCAGGC TCTTATGAGA GAGAGACATT TGCTCTGTCT
    TCCCTGGAAA CAGTCACGGA GGCTCTTTTG GAGATCATGG AGGCCTGCAT GAGGGACATT
    CCTGGATGCA AATGGCTAGA TCAGTGGACT GAATTGGCGC AAAAGTTTGC GTTCCAGTAT
    AACCCCTCTC TTCAGCCTCG GGCGCTGGTG GTGTTCGGCT GCATCAGTAA ACGAGTGACT
    CACGGACAGC TAAAGCAGAT CATTCGTATT CTCAGTAAAG CCAGTCCTCT ACTCAGCATT
    GATCGGGGGC TGGAGAGCTG CTTAAAGGGC CCAGATAATT ACAACAGCCA GGTTCTCATC
    GAAGCCACAG TCATCGCTCT CACCAAACTA CAGCCTCTCC TCGATCAGGA TTCTCCTATG
    CATAAAGCTC TGTTCTGGGT GGCCGTGGCC GTGCTTCAGT TGGATGAAGT GAATCTGTAC
    TCCGCCGGCA CTGCCTTACT GGAGCAGAAC CTGCACACAC TGGACAGCCT CCGAGTGTTT
    AATGATAAGA GTCCTGAAGA GGTTTTTATG GAGATCCGGA AACCTCTAGA GTGGCACTGT
    AAACAAATGG ACCATTTTGT GGGACTCAAC TTTGCCTCCA ACTTCAATTT TGCATTAGTG
    GGCCATTTAC TTAAAGGATA CAGACATCCC TCTTCAACAA CAGTTGCCAG GACAGTTCGT
    ATCCTGCATA CACTCCTGGG CCTGGTGGGA AAACACCGCA GCTGTGACAA GTTTGAGGTG
    AACACGCAGA GTGTGGCCTA TCTGGCAGCT TTGCTCACAG TGTCAGAGGA AGTTAGGAGC
    CGCTGCAGTT TAAAGCACAG GAAATCATTG CTGCTTTCAG AAGTTACAAT GGAGAACTTC
    CCCATGGACA CATACACCGT TTACGACACT GAACCCGGGT GCAGGCTACT GCGTGAAAAC
    CAGCCTTGGG CTTCTCCGAA AGTGTCAGAG CGCAGTCTTG CACCTCCGTA CTCCACAATA
    GGGCAGACCA GCCTTCGTCC ACGCAAATCC ATGAGTGTAG ACATGGGCCA GCCGTCTCAG
    GCCAATGCGA AGAAACTACT GGGTATTAGC GGCACTAGAA AGAGTTTCGA TCACTTAATA
    TCAGAATCCA AAGCCCCAAA AAGACCAGAC ATGGAGTCGG GCATCACCAC ACCTCCAAAG
    ATGAGGCGGG TTTCTGAAAA TGACTATGAG ATCGAAACTC AGCGAATAGC CAACTCTCAT
    TTACGCAAAG TGTCTGTGTC TGAATCCAAT GTCCTCCTGG ACGAGAACGT ACTTACGGAC
    CCCAAGATCC AGACGTTGCT CCTCACTGTA CTGGCAACAC TGGTGAAGTA CACTACAGAC
    GAGTTTCATC AGCGGATCTT GTATGAGTAT TTGGCTGAGG CCAGTGTGGT CTTCCCCAAG
    GTCTTTCCAG TTGTGCACAA CCTGCTGGAC TCCAAGTTAA GCACCGTCCT TTCCATGTGC
    CAGGACCCGA ACCTTCTGAA CCCCGTCCAT GGCATCATTC AGAACGTGGT CTATCATGAG
    GAGTCTCCCC CTCAGTACCA GCCGTCCTGC TTGCAGAGCT TTGGTTTCAA TGGTCTTTGG
    AGGTTTGCCG GGCCGTTCTC TAAGCAAACC CAAATTCCAG ACTACGCCGA GCTGGTGGTT
    AAATTTTTGG ATGCTTTGAT TGACACGTAT CTCCCGGGCA TTGAGGAGGA AGGAGGAGAA
    GAGTCACTGC GGACCCCGAC CTCGCCATAT CCACCAACCG TCCAGAGCCA GCTGAGCATC
    ACAGCCAATC TCAACCTGTC CAACTCCATG ACATCACTTG CCACATCACA GCACTCCCCA
    GCCTGTCTGC CTTGCTCTAA GTCGGCTGTA TTCCCACCTC ACCTCGTCCT GCCAGGCATC
    GACAAGGAGA ACGTTGAGAT TTCTCCCACC TCGAGTCTGT CCACCAGTGG GCGAATGCGG
    CACGGCTCAG CAAGCCAGGT GCAGAAGCAG CGCAGCGCAG GCAGCTTCAA ACGGCCAAGC
    ATCAAAAAGA TCGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322123.1
    CDS247..8664
    Translation

    Target ORF information:

    RefSeq Version XM_021466448.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio neurofibromin 1a (nf1a), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466448.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGGCGGCGC ACAAGCCCGT GGAATGGGTC CAGGCCATCA TTAACAGATT CGACGAACAG 
    CTCCCAATCA AAACAGGACA GCAGAGCACC CACACCAAAG TGAGTACAGA GCACAACAAA
    GAATGTTTGA TCAACATCTC CAAGTACAAA TTTGCCCTCG TCATCAATGG CCTGACCAAC
    ATACTCAAAA ATGTGAACAA CATGCGGATA TTTGGCGAAG CTGCTGAGAA GAACCTCTAC
    TTCTCCCAGC TGATCATACT GGACACACTG GAGAAGTGTC TAGCAGGGCA ACCTAAGGAT
    TGCCTGAGGC TGGATGAGGC TATGTTGGTA AAACAGTTGC TGCCTGAAAT CTGTCACTTC
    CTGCACTCGT ACCGTGAGGG CCAGCAACAC GCCACTCAGC TGCGCAGCTC CGCCTCTGCT
    GTGCTTTTCT CTCTCAGCTG CAACAACTTT AATGCCGTCT TCAGTCGTAT CTCCACCAGG
    TTGCAAGAGC TCACAGTTTG TACAGAGGAC ACAGTGGATG TCCATGACAT TGAGCTCATT
    CAGTATATCA ATGTAGATTG CTCCAAGTTA AAGAGACTCC TACAAGAAGC AGTACTTAAG
    TTCAAGTCTT TGAAAAAGCC GGCTCAGCTT GCAGTAATCA GCAGTTTGGA AAAGGCTTTC
    TGGAACTGGG TTGAACACTA CCCTGATGAT TTCACTATGC TCTATCAGAG ACCCCAGGCC
    GACATGGCCG ACTGTGCAGA GAGACTGTTC GACCTAGTGG ACAGTTTTGC GGAAAGCGCA
    AAGAGAAAAG CTGCTGTGTG GCCACTGCAG ATCATCCTCC TCATCCTCTG TCCAGAGATC
    ACGCAGAGCA TCACCAAAGA AGTAGGGGGA GAGGAGAAAG CTAACAAGAA GTCATTTGTA
    GACAGTTTAC GAAAGGCTCT TGGCCAACAC AGCGGCAGCA AACAATTGAC GGAAAGTGCA
    GCTGTTGCAT GCGTCAAACT CTGCAAAGCC TGCACATATA TCAACTGGGA GGATAATTCT
    GTCATCTTTC TCCTGGTTCA GTCCATCGTC ATGGACCTTA AGGGTCTCCT GTTTAACCCC
    ACCAAACCTT TCTCAAGAGG TACGGGCTGC CAGAGCGCCG ATGTAGATTT GATGATCGAC
    TGCTTTGTGT CATGTTATCG CATCAATCCT CGCAACAACC AGCATTTTAA GGTGTGTCTG
    GCCTCGACTT CTCCTCCTAC CTTCCATTTC GTCCTTGTCA ACTCCCTGCA CAAAATTATC
    ACAAATTCTC ACCTGGACTG GTGGCCGAAG ATTGACGCCA TCTACTGCTA CTCAGGAGAA
    CTACGCACCA TGTTTGCAGA CACACTGGGT CGAGTGATTC AGAGTCTCAG TGCACATCCG
    CCTCTGAGGA TGACATCAAG TTTGACATTC AAAGAGAAAA TGACAACAAG TTTGAAGTTC
    AAGGAAAAGT CCACAGATCT TGATTCGCGT TCTTTGCTGC TGGCACTGGT CAAACTTATC
    CACGCTGACC CCAAACTAAT GCTGCACAAT CCGGGAAAAC CTGGTCAGGA GATTCAGAAC
    AGCACAGCTG AGATCATCAA AGGTCTGGTT CAGCTGGTTC CTCTTACCCA CAGTGCTGAG
    CTTTCCCAAG AGGCCATGGA GGCATTGCTT GTTTTACATC AGCCAGAAAC CATTGAGCTA
    TGGAACCCGG ACGCTCCCAT TGAGACCTTC TGGGACATCA GCTCTCAGGT GTTATTTCTA
    ATTTGTAAGA AGCTGATTGG ACACCAGATG ATCAACAGTA CAGAGATACT CAAGTGGCTC
    AGGGAGATTC TCATATGCAG GAACAAGTTT CTATTAAAGA ACAAGGAGTG TGCTACACAG
    GGCAGTCTGA TCCCCATCAG CAGACAGGCT CAGACCAAGC TGGAGGTGTG TCTGTACACG
    TTCCTGTGGA GTCCTGATGC AGAGGCTGTT TTGGTGGCCA TGTCCTGTTT CAGGCATCTC
    TGTGAGGAGG CCGATATTCG CTGCAGTGTC GATGAGCTGC CTGTTCACAC AGTCCTACCC
    AATTACAACA CCTTCATTGA GCTTGCATCC GTCAGCAATA TGATGGCCAC AGGTCGCAAC
    ACACTACAAA AAAGAGTGAT GGCACTTTTA AGAAGAATAA AGCACCCTAC AGCTGGAAAT
    GTAGAGGCAT GGGAAGACAC ATATGCAAAG TGGGAGCAAG CTACAAAACA GATACTCAAC
    CACCCAAAAA ACAAAGTGGA AGATGGACAG GTAGTTAACA TAAGATTGGT CAATTATTTC
    ATCTTTTTTT CCATTCATGG TCATCTATTT TCTGTTCGTC CTCAGGAGTG GATCAACATG
    ACTGGTTTCT TGTGCGCTCT GGGAGGTGTT TGTCTCCAAC AGCGCAGCAC GCCGAGTCTG
    GGCACCTACA GTCCTCCAAT GGGCCCCCTG ACCGAACGCA AGGGGTCCAT GATTTCCATG
    AGCTCCTGCG AGGTAAACAG CGAAACATCC CTGAATCGCT TTCTGGAGCG TCTGCTGGCT
    CTGATGGTCT GCACACATGA GAAAGTGGGA GTGCAAATTC GCACCAACGT CAAAGACCTG
    GTGGGACTGG AGCTCAGTCC GGCTCTCTAT TCAATGCTCT TCACTAAGCT GCGCAACAGC
    ATCGGACGCT TCTTTGACAC CCAGGGGCCG GTTCCTATTA CAGATGCAAA CACTCTGTTT
    GTAGAGCAGA CCATTGCTAT CATGAAGAAC TTGCTGGACA GTCACACTGA AGGAAGCTCA
    GAGCACCTTG GTCAAGCCAG CATTGAGACC ATGATGCTCA ATCTAGTTAG GTATGTGCGT
    ATTTTAGGGA ACATTGTTCA TGCCATTCAG ATCAAAACCA AGTTGTGTCA GTTAGTGGAG
    GTGATGATGG AGCGCAGGGA TGACCTCTCC TTCTGCCAGG AGATGAAATT CAGGAATAAA
    ATGGTGGAGT ACCTCACAGA CTGGGTGATG GGAACCTCCA ATCAAGCTGC GGACGATGAT
    GTCAAATGCC TGACCCGCGA TCTGGACCAG GCCAGTATGG AGGCTGTCGT GTCTCTGTTG
    GCTGGTCTTC CTCTACAGCC TGAGGAGGGT GACGGTGTTG AGCTCATGGA GGCCAAATCA
    CAGCTCTTCC TGAAGTACTT CACACTGTTC ATGAACCTGT TGAATGACTG CAGCGAGGTT
    GAGGAAGATG GCCAGCAGGT CGCAGGGAGG AAACGTGGCA TGTCTAGACG ACTGGCATCT
    CTGCGGCACT GCACAGTATT GGCTATGTCT AACTTGCTCA ATGCTAATGT GGATAGCGGC
    CTCATGCACT CAATCGGTCT GGGATACCAT AAGGACCTGC AGACACGGGC TACATTTATG
    GAAGTGCTCA CTAAGATCCT TCAGCAAGGG ACGGAGTTCG ACACGCTTGC AGAGACTGTG
    CTTGCAGATC GCTTTGAGAG ACTGGTCGAG CTGGTCACCA TGATGGGAGA TCAGGGAGAG
    CTGCCCATAG CCATGGCTCT GGCCAATGTG GTCCCATGCT CTCAATGGGA TGAGCTGGCC
    AGAGTGTTAG TGACTCTCTT CGACTCTCGC CATCTGCTGT ATCAGCTGTT GTGGAATATG
    TTCTCCAAAG AGGTGGAGCT GGCAGACTCA ATGCAGACGC TCTTTAGAGG AAATAGCCTA
    GCCAGCAAGA TTATGACCTT CTGCTTCAAG GTGTACGGAG CTGCGTATCT ACAGAAGCTT
    CTGGAGCCGC TGTTGAGAGG AATCATCACT ACACCAGAGT GGCAGCAGAT CAGTTTTGAA
    GTGGACCCTA CTAGACTTGA ACAGGCAGAG AACCTGGAGG AGAACCAGAA GAACCTGCTT
    CAGATCACTG ACCGCTTCTT CCTGGCCATC ATCAACTCCT CGAGCGAGTT TCCTCCACAG
    CTGCGCAGTG TGTGCCACTG TCTGTATCAG GCTACTTGCC ACTCCCTTCT GAATAAAGCC
    ACTGTTAAAG AAAAAAAGGA AAACAAAAAA TCCGTGGTCA GTCAGCGGTT TCCTCAGAAC
    AGCATTGGTG CTGTAGGAAG TGCCATGTTC CTGCGCTTTA TTAACCCAGC TATCGTGTCC
    CCATATGAGG CTGGAATCCT AGACAAGAAA CCCCCACCTC GCATCGAGCG TGGCCTTAAA
    CTCATGTCTA AGATTCTGCA GAGCATTGCT AATCATGTTC TCTTCACAAA AGAGGAGCAT
    ATGAGGCCCT TTAATGATTT TGTCAAAAGC AACTTCGATG CAGCCAGAAG GTTCTTCTTG
    GATATCGCAT CTGATTCTCC ACCAAGTGAT TCTGTGAACC ATAGTCTGTC TTTCATCAGT
    GACGGTAATG TGCTCGCTCT GCACCGGCTA TTATGGAACA ACCAGGAAAG AATTGGCCAA
    TACCTGTCCA GCAACAGGGA CCACAAGGCA GTGGGCCGTA GACCATTTGA CAAGATGGCC
    ACTCTTCTGG CGTACCTGGG TCCTCCTGAA CATAAGCCAG TAGCAGACAC ACACTGGTCC
    AGCCTCAACC TCACCAGCTC AAAGTTTGAG GAGTTCATGA CAAGGCATCA AGTCCATGAG
    AAGGAGGAAT TTAAAGCATT AAAAACCCTC AACATATTTT ACCAAGCTGG CACATCTAAG
    AACGGCAACC CTGTGTTTTA TTACATCGCA CGCAGGTTCA AAACTGGACA AATCAATGGT
    GATCTTCTGA TCTACCACGT CCTGCTGACG CTCAAGCCCT ACTATGCCAA GCATTATGAG
    ATCGTAGTTG ACCTTACTCA TGTGGGCCCC AGTAACCGTT TCAAGACGGA CTTCTTATCC
    AAATGGTTTG TGGTCTTTCC AAACTTCGCC TATGAGAACG TGGCTGCTGT GTACATCTAC
    AACTGCAACA CCTGGGTACG CGAGTACACC AAATACCACG AGCGACTGCT GACTGGCCTC
    AAGGGGAGCA AGAAGCTGGT GTTTATCGAT TCGCCTGCAC GTCTAGCTGA ACACATCGAA
    CCCGAACAGC AGAAGCTGCC CGCAGCTACT TTAGCACTGG AAGAAGACCT CAAAGTGTTC
    CACAACGCCC TAAAGCTTGC ACACAGAGAC ACAAAAGTGT CAATTAAGGT GGGATCCACA
    GCTGTACAGG TGACCTCGGC CGAAAGAACG CGTGTCCTAG GACAGCCTGT GTTCCTAAAT
    GACATCTACT ACGCTTCAGA GATCGAGGAG ATATGCCTGG TGGACGAGAA CCAGTTCACG
    CTAACCATCG CTAACCAGGG CACCCCACTC ACTTTCATGC ACCAGGAGTG TGAAGCCATC
    GTTCAGTCCA TCATCCACAT CCGCACGCGC TGGGAACTTT CCCAACCAGA CTCCATTCCC
    CAACACACCA AAATCAGGCC TAAGGATGTT CCTGGCACCC TGCTCAACAT CGCTCTTCTC
    AACCTGGGAA GCTCCGACCC CAGTCTGAGG TCTGCGGCCT ACAATCTCCT TTGTGCCTTA
    ACCTGCACCT TCAACCTGAA GATTGAAGGC CAGCTTCTGG AAACGTCTGG CTTGTGCATC
    CCAGCCAACA ACACGCTTTT TATTGTGTCC ATTAGCAAAA CGCTAGCAGC TAATGAACCC
    CACCTCACTC TGGAGTTCCT GGAAGAGTGC ATCTCAGGAT TCAGTAAATC AAGTATAGAG
    CTGAAGCATC TGTGTCTAGA ATATATGACT CCTTGGCTCC GTAATTTGGT GCGCTTTTGC
    AAACACAATG ATGACGCCAA ACGGCAGCGT GTCACGGCCA TCCTGGACAA GCTTATTACT
    ATGACCATCA ACGAGAAGCA GATGTACCCC TCCATACAGG CCAAGATATG GGGCAGCTTG
    GGTCAGATCA CAGACCTGCT TGATGTGGTG TTGGACAGCT TTATAAAGAC CAGCGCTACT
    GGCGGTTTAG GATCCATCAA AGCTGAAGTG ATGGCTGACA CTGCTGTAGC TTTGGCGTCA
    GGAAATGTCA AACTGGTCTC CAGCAAGGTG ATTGGCCGAA TGTGTAAGAT CATCGACAAG
    ACGTGTCTGT CTCCCACTCC GACCCTTGAG CAGCACCTCA TGTGGGACGA CATCGCCATC
    CTGGCCCGGT ACATGCTCAT GCTGTCCTTC AACAACTCTC TGGACGTGGC CGCTCATCTG
    CCATACCTGT TCCACGTCGT CACCCTGCTG GTGGCCACGG GCCCTCTCTC GCTGCGTGCA
    TCAACACACG GCCTGGTCAT CAACATCATT CACTCGCTCT GCACATGCTC ACAACTCAAC
    TTCAGTGAGG AGACTAAACA GGTGCTGCGC CTGAGTCTGA CTGAGTTCTC CCTGCCCAAG
    TTCTACCTGC TGTTCGGCAT CAGTAAGGTC AAATCTGCGG CGGTCATCGC TTTCCGTTCC
    AGCTACAGAG ACCGCTCATT CTCTCCAGGC TCTTATGAGA GAGAGACATT TGCTCTGTCT
    TCCCTGGAAA CAGTCACGGA GGCTCTTTTG GAGATCATGG AGGCCTGCAT GAGGGACATT
    CCTGGATGCA AATGGCTAGA TCAGTGGACT GAATTGGCGC AAAAGTTTGC GTTCCAGTAT
    AACCCCTCTC TTCAGCCTCG GGCGCTGGTG GTGTTCGGCT GCATCAGTAA ACGAGTGACT
    CACGGACAGC TAAAGCAGAT CATTCGTATT CTCAGTAAAG CCAGTCCTCT ACTCAGCATT
    GATCGGGGGC TGGAGAGCTG CTTAAAGGGC CCAGATAATT ACAACAGCCA GGTTCTCATC
    GAAGCCACAG TCATCGCTCT CACCAAACTA CAGCCTCTCC TCGATCAGGA TTCTCCTATG
    CATAAAGCTC TGTTCTGGGT GGCCGTGGCC GTGCTTCAGT TGGATGAAGT GAATCTGTAC
    TCCGCCGGCA CTGCCTTACT GGAGCAGAAC CTGCACACAC TGGACAGCCT CCGAGTGTTT
    AATGATAAGA GTCCTGAAGA GGTTTTTATG GAGATCCGGA AACCTCTAGA GTGGCACTGT
    AAACAAATGG ACCATTTTGT GGGACTCAAC TTTGCCTCCA ACTTCAATTT TGCATTAGTG
    GGCCATTTAC TTAAAGGATA CAGACATCCC TCTTCAACAA CAGTTGCCAG GACAGTTCGT
    ATCCTGCATA CACTCCTGGG CCTGGTGGGA AAACACCGCA GCTGTGACAA GTTTGAGGTG
    AACACGCAGA GTGTGGCCTA TCTGGCAGCT TTGCTCACAG TGTCAGAGGA AGTTAGGAGC
    CGCTGCAGTT TAAAGCACAG GAAATCATTG CTGCTTTCAG AAGTTACAAT GGAGAACTTC
    CCCATGGACA CATACACCGT TTACGACACT GAACCCGGGT GCAGGCTACT GCGTGAAAAC
    CAGCCTTGGG CTTCTCCGAA AGTGTCAGAG CGCAGTCTTG CACCTCCGTA CTCCACAATA
    GGGCAGACCA GCCTTCGTCC ACGCAAATCC ATGAGTGTAG ACATGGGCCA GCCGTCTCAG
    GCCAATGCGA AGAAACTACT GGGTATTAGC GGCACTAGAA AGAGTTTCGA TCACTTAATA
    TCAGAATCCA AAGCCCCAAA AAGACCAGAC ATGGAGTCGG GCATCACCAC ACCTCCAAAG
    ATGAGGCGGG TTTCTGAAAA TGACTATGAG ATCGAAACTC AGCGAATAGC CAACTCTCAT
    TTACGCAAAG TGTCTGTGTC TGAATCCAAT GTCCTCCTGG ACGAGAACGT ACTTACGGAC
    CCCAAGATCC AGACGTTGCT CCTCACTGTA CTGGCAACAC TGGTGAAGTA CACTACAGAC
    GAGTTTCATC AGCGGATCTT GTATGAGTAT TTGGCTGAGG CCAGTGTGGT CTTCCCCAAG
    GTCTTTCCAG TTGTGCACAA CCTGCTGGAC TCCAAGTTAA GCACCGTCCT TTCCATGTGC
    CAGGACCCGA ACCTTCTGAA CCCCGTCCAT GGCATCATTC AGAACGTGGT CTATCATGAG
    GAGTCTCCCC CTCAGTACCA GCCGTCCTGC TTGCAGAGCT TTGGTTTCAA TGGTCTTTGG
    AGGTTTGCCG GGCCGTTCTC TAAGCAAACC CAAATTCCAG ACTACGCCGA GCTGGTGGTT
    AAATTTTTGG ATGCTTTGAT TGACACGTAT CTCCCGGGCA TTGAGGAGGA AGGAGGAGAA
    GAGTCACTGC GGACCCCGAC CTCGCCATAT CCACCAACCG TCCAGAGCCA GCTGAGCATC
    ACAGCCAATC TCAACCTGTC CAACTCCATG ACATCACTTG CCACATCACA GCACTCCCCA
    GCCTGTCTGC CTTGCTCTAA GTCGGCTGTA TTCCCACCTC ACCTCGTCCT GCCAGGCATC
    GACAAGGAGA ACGTTGAGAT TTCTCCCACC TCGAGTCTGT CCACCAGTGG GCGAATGCGG
    CACGGCTCAG CAAGCCAGGT GCAGAAGCAG CGCAGCGCAG GCAGCTTCAA ACGGCCAAGC
    ATCAAAAAGA TCGTCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa39876
    Clone ID Related Accession (Same CDS sequence) XM_021466449.1
    Accession Version XM_021466449.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8364bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product neurofibromin isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
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    1321
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    1441
    1501
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    1621
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    8341
    ATGGCGGCGC ACAAGCCCGT GGAATGGGTC CAGGCCATCA TTAACAGATT CGACGAACAG 
    CTCCCAATCA AAACAGGACA GCAGAGCACC CACACCAAAG TGAGTACAGA GCACAACAAA
    GAATGTTTGA TCAACATCTC CAAGTACAAA TTTGCCCTCG TCATCAATGG CCTGACCAAC
    ATACTCAAAA ATGTGAACAA CATGCGGATA TTTGGCGAAG CTGCTGAGAA GAACCTCTAC
    TTCTCCCAGC TGATCATACT GGACACACTG GAGAAGTGTC TAGCAGGGCA ACCTAAGGAT
    TGCCTGAGGC TGGATGAGGC TATGTTGGTA AAACAGTTGC TGCCTGAAAT CTGTCACTTC
    CTGCACTCGT ACCGTGAGGG CCAGCAACAC GCCACTCAGC TGCGCAGCTC CGCCTCTGCT
    GTGCTTTTCT CTCTCAGCTG CAACAACTTT AATGCCGTCT TCAGTCGTAT CTCCACCAGG
    TTGCAAGAGC TCACAGTTTG TACAGAGGAC ACAGTGGATG TCCATGACAT TGAGCTCATT
    CAGTATATCA ATGTAGATTG CTCCAAGTTA AAGAGACTCC TACAAGAAGC AGTACTTAAG
    TTCAAGTCTT TGAAAAAGCC GGCTCAGCTT GCAGTAATCA GCAGTTTGGA AAAGGCTTTC
    TGGAACTGGG TTGAACACTA CCCTGATGAT TTCACTATGC TCTATCAGAG ACCCCAGGCC
    GACATGGCCG ACTGTGCAGA GAGACTGTTC GACCTAGTGG ACAGTTTTGC GGAAAGCGCA
    AAGAGAAAAG CTGCTGTGTG GCCACTGCAG ATCATCCTCC TCATCCTCTG TCCAGAGATC
    ACGCAGAGCA TCACCAAAGA AGTAGGGGGA GAGGAGAAAG CTAACAAGAA GTCATTTGTA
    GACAGTTTAC GAAAGGCTCT TGGCCAACAC AGCGGCAGCA AACAATTGAC GGAAAGTGCA
    GCTGTTGCAT GCGTCAAACT CTGCAAAGCC TGCACATATA TCAACTGGGA GGATAATTCT
    GTCATCTTTC TCCTGGTTCA GTCCATCGTC ATGGACCTTA AGGGTCTCCT GTTTAACCCC
    ACCAAACCTT TCTCAAGAGG TACGGGCTGC CAGAGCGCCG ATGTAGATTT GATGATCGAC
    TGCTTTGTGT CATGTTATCG CATCAATCCT CGCAACAACC AGCATTTTAA GGTGTGTCTG
    GCCTCGACTT CTCCTCCTAC CTTCCATTTC GTCCTTGTCA ACTCCCTGCA CAAAATTATC
    ACAAATTCTC ACCTGGACTG GTGGCCGAAG ATTGACGCCA TCTACTGCTA CTCAGGAGAA
    CTACGCACCA TGTTTGCAGA CACACTGGGT CGAGTGATTC AGAGTCTCAG TGCACATCCG
    CCTCTGAGGA TGACATCAAG TTTGACATTC AAAGAGAAAA TGACAACAAG TTTGAAGTTC
    AAGGAAAAGT CCACAGATCT TGATTCGCGT TCTTTGCTGC TGGCACTGGT CAAACTTATC
    CACGCTGACC CCAAACTAAT GCTGCACAAT CCGGGAAAAC CTGGTCAGGA GATTCAGAAC
    AGCACAGCTG AGATCATCAA AGGTCTGGTT CAGCTGGTTC CTCTTACCCA CAGTGCTGAG
    CTTTCCCAAG AGGCCATGGA GGCATTGCTT GTTTTACATC AGCCAGAAAC CATTGAGCTA
    TGGAACCCGG ACGCTCCCAT TGAGACCTTC TGGGACATCA GCTCTCAGGT GTTATTTCTA
    ATTTGTAAGA AGCTGATTGG ACACCAGATG ATCAACAGTA CAGAGATACT CAAGTGGCTC
    AGGGAGATTC TCATATGCAG GAACAAGTTT CTATTAAAGA ACAAGGAGTG TGCTACACAG
    GGCAGTCTGA TCCCCATCAG CAGACAGGCT CAGACCAAGC TGGAGGTGTG TCTGTACACG
    TTCCTGTGGA GTCCTGATGC AGAGGCTGTT TTGGTGGCCA TGTCCTGTTT CAGGCATCTC
    TGTGAGGAGG CCGATATTCG CTGCAGTGTC GATGAGCTGC CTGTTCACAC AGTCCTACCC
    AATTACAACA CCTTCATTGA GCTTGCATCC GTCAGCAATA TGATGGCCAC AGGTCGCAAC
    ACACTACAAA AAAGAGTGAT GGCACTTTTA AGAAGAATAA AGCACCCTAC AGCTGGAAAT
    GTAGAGGCAT GGGAAGACAC ATATGCAAAG TGGGAGCAAG CTACAAAACA GATACTCAAC
    CACCCAAAAA ACAAAGTGGA AGATGGACAG GTAGTTAACA TAAGATTGGT CAATTATTTC
    ATCTTTTTTT CCATTCATGG TCATCTATTT TCTGTTCGTC CTCAGGAGTG GATCAACATG
    ACTGGTTTCT TGTGCGCTCT GGGAGGTGTT TGTCTCCAAC AGCGCAGCAC GCCGAGTCTG
    GGCACCTACA GTCCTCCAAT GGGCCCCCTG ACCGAACGCA AGGGGTCCAT GATTTCCATG
    AGCTCCTGCG AGGTAAACAG CGAAACATCC CTGAATCGCT TTCTGGAGCG TCTGCTGGCT
    CTGATGGTCT GCACACATGA GAAAGTGGGA GTGCAAATTC GCACCAACGT CAAAGACCTG
    GTGGGACTGG AGCTCAGTCC GGCTCTCTAT TCAATGCTCT TCACTAAGCT GCGCAACAGC
    ATCGGACGCT TCTTTGACAC CCAGGGGCCG GTTCCTATTA CAGATGCAAA CACTCTGTTT
    GTAGAGCAGA CCATTGCTAT CATGAAGAAC TTGCTGGACA GTCACACTGA AGGAAGCTCA
    GAGCACCTTG GTCAAGCCAG CATTGAGACC ATGATGCTCA ATCTAGTTAG GTATGTGCGT
    ATTTTAGGGA ACATTGTTCA TGCCATTCAG ATCAAAACCA AGTTGTGTCA GTTAGTGGAG
    GTGATGATGG AGCGCAGGGA TGACCTCTCC TTCTGCCAGG AGATGAAATT CAGGAATAAA
    ATGGTGGAGT ACCTCACAGA CTGGGTGATG GGAACCTCCA ATCAAGCTGC GGACGATGAT
    GTCAAATGCC TGACCCGCGA TCTGGACCAG GCCAGTATGG AGGCTGTCGT GTCTCTGTTG
    GCTGGTCTTC CTCTACAGCC TGAGGAGGGT GACGGTGTTG AGCTCATGGA GGCCAAATCA
    CAGCTCTTCC TGAAGTACTT CACACTGTTC ATGAACCTGT TGAATGACTG CAGCGAGGTT
    GAGGAAGATG GCCAGCAGGT CGCAGGGAGG AAACGTGGCA TGTCTAGACG ACTGGCATCT
    CTGCGGCACT GCACAGTATT GGCTATGTCT AACTTGCTCA ATGCTAATGT GGATAGCGGC
    CTCATGCACT CAATCGGTCT GGGATACCAT AAGGACCTGC AGACACGGGC TACATTTATG
    GAAGTGCTCA CTAAGATCCT TCAGCAAGGG ACGGAGTTCG ACACGCTTGC AGAGACTGTG
    CTTGCAGATC GCTTTGAGAG ACTGGTCGAG CTGGTCACCA TGATGGGAGA TCAGGGAGAG
    CTGCCCATAG CCATGGCTCT GGCCAATGTG GTCCCATGCT CTCAATGGGA TGAGCTGGCC
    AGAGTGTTAG TGACTCTCTT CGACTCTCGC CATCTGCTGT ATCAGCTGTT GTGGAATATG
    TTCTCCAAAG AGGTGGAGCT GGCAGACTCA ATGCAGACGC TCTTTAGAGG AAATAGCCTA
    GCCAGCAAGA TTATGACCTT CTGCTTCAAG GTGTACGGAG CTGCGTATCT ACAGAAGCTT
    CTGGAGCCGC TGTTGAGAGG AATCATCACT ACACCAGAGT GGCAGCAGAT CAGTTTTGAA
    GTGGACCCTA CTAGACTTGA ACAGGCAGAG AACCTGGAGG AGAACCAGAA GAACCTGCTT
    CAGATCACTG ACCGCTTCTT CCTGGCCATC ATCAACTCCT CGAGCGAGTT TCCTCCACAG
    CTGCGCAGTG TGTGCCACTG TCTGTATCAG GCTACTTGCC ACTCCCTTCT GAATAAAGCC
    ACTGTTAAAG AAAAAAAGGA AAACAAAAAA TCCGTGGTCA GTCAGCGGTT TCCTCAGAAC
    AGCATTGGTG CTGTAGGAAG TGCCATGTTC CTGCGCTTTA TTAACCCAGC TATCGTGTCC
    CCATATGAGG CTGGAATCCT AGACAAGAAA CCCCCACCTC GCATCGAGCG TGGCCTTAAA
    CTCATGTCTA AGATTCTGCA GAGCATTGCT AATCATGTTC TCTTCACAAA AGAGGAGCAT
    ATGAGGCCCT TTAATGATTT TGTCAAAAGC AACTTCGATG CAGCCAGAAG GTTCTTCTTG
    GATATCGCAT CTGATTCTCC ACCAAGTGAT TCTGTGAACC ATAGTCTGTC TTTCATCAGT
    GACGGTAATG TGCTCGCTCT GCACCGGCTA TTATGGAACA ACCAGGAAAG AATTGGCCAA
    TACCTGTCCA GCAACAGGGA CCACAAGGCA GTGGGCCGTA GACCATTTGA CAAGATGGCC
    ACTCTTCTGG CGTACCTGGG TCCTCCTGAA CATAAGCCAG TAGCAGACAC ACACTGGTCC
    AGCCTCAACC TCACCAGCTC AAAGTTTGAG GAGTTCATGA CAAGGCATCA AGTCCATGAG
    AAGGAGGAAT TTAAAGCATT AAAAACCCTC AACATATTTT ACCAAGCTGG CACATCTAAG
    AACGGCAACC CTGTGTTTTA TTACATCGCA CGCAGGTTCA AAACTGGACA AATCAATGGT
    GATCTTCTGA TCTACCACGT CCTGCTGACG CTCAAGCCCT ACTATGCCAA GCATTATGAG
    ATCGTAGTTG ACCTTACTCA TGTGGGCCCC AGTAACCGTT TCAAGACGGA CTTCTTATCC
    AAATGGTTTG TGGTCTTTCC AAACTTCGCC TATGAGAACG TGGCTGCTGT GTACATCTAC
    AACTGCAACA CCTGGGTACG CGAGTACACC AAATACCACG AGCGACTGCT GACTGGCCTC
    AAGGGGAGCA AGAAGCTGGT GTTTATCGAT TCGCCTGCAC GTCTAGCTGA ACACATCGAA
    CCCGAACAGC AGAAGCTGCC CGCAGCTACT TTAGCACTGG AAGAAGACCT CAAAGTGTTC
    CACAACGCCC TAAAGCTTGC ACACAGAGAC ACAAAAGTGT CAATTAAGGT GGGATCCACA
    GCTGTACAGG TGACCTCGGC CGAAAGAACG CGTGTCCTAG GACAGCCTGT GTTCCTAAAT
    GACATCTACT ACGCTTCAGA GATCGAGGAG ATATGCCTGG TGGACGAGAA CCAGTTCACG
    CTAACCATCG CTAACCAGGG CACCCCACTC ACTTTCATGC ACCAGGAGTG TGAAGCCATC
    GTTCAGTCCA TCATCCACAT CCGCACGCGC TGGGAACTTT CCCAACCAGA CTCCATTCCC
    CAACACACCA AAATCAGGCC TAAGGATGTT CCTGGCACCC TGCTCAACAT CGCTCTTCTC
    AACCTGGGAA GCTCCGACCC CAGTCTGAGG TCTGCGGCCT ACAATCTCCT TTGTGCCTTA
    ACCTGCACCT TCAACCTGAA GATTGAAGGC CAGCTTCTGG AAACGTCTGG CTTGTGCATC
    CCAGCCAACA ACACGCTTTT TATTGTGTCC ATTAGCAAAA CGCTAGCAGC TAATGAACCC
    CACCTCACTC TGGAGTTCCT GGAAGAGTGC ATCTCAGGAT TCAGTAAATC AAGTATAGAG
    CTGAAGCATC TGTGTCTAGA ATATATGACT CCTTGGCTCC GTAATTTGGT GCGCTTTTGC
    AAACACAATG ATGACGCCAA ACGGCAGCGT GTCACGGCCA TCCTGGACAA GCTTATTACT
    ATGACCATCA ACGAGAAGCA GATGTACCCC TCCATACAGG CCAAGATATG GGGCAGCTTG
    GGTCAGATCA CAGACCTGCT TGATGTGGTG TTGGACAGCT TTATAAAGAC CAGCGCTACT
    GGCGGTTTAG GATCCATCAA AGCTGAAGTG ATGGCTGACA CTGCTGTAGC TTTGGCGTCA
    GGAAATGTCA AACTGGTCTC CAGCAAGGTG ATTGGCCGAA TGTGTAAGAT CATCGACAAG
    ACGTGTCTGT CTCCCACTCC GACCCTTGAG CAGCACCTCA TGTGGGACGA CATCGCCATC
    CTGGCCCGGT ACATGCTCAT GCTGTCCTTC AACAACTCTC TGGACGTGGC CGCTCATCTG
    CCATACCTGT TCCACGTCGT CACCCTGCTG GTGGCCACGG GCCCTCTCTC GCTGCGTGCA
    TCAACACACG GCCTGGTCAT CAACATCATT CACTCGCTCT GCACATGCTC ACAACTCAAC
    TTCAGTGAGG AGACTAAACA GGTGCTGCGC CTGAGTCTGA CTGAGTTCTC CCTGCCCAAG
    TTCTACCTGC TGTTCGGCAT CAGTAAGGTC AAATCTGCGG CGGTCATCGC TTTCCGTTCC
    AGCTACAGAG ACCGCTCATT CTCTCCAGGC TCTTATGAGA GAGAGACATT TGCTCTGTCT
    TCCCTGGAAA CAGTCACGGA GGCTCTTTTG GAGATCATGG AGGCCTGCAT GAGGGACATT
    CCTGGATGCA AATGGCTAGA TCAGTGGACT GAATTGGCGC AAAAGTTTGC GTTCCAGTAT
    AACCCCTCTC TTCAGCCTCG GGCGCTGGTG GTGTTCGGCT GCATCAGTAA ACGAGTGACT
    CACGGACAGC TAAAGCAGAT CATTCGTATT CTCAGTAAAG CCAGTCCTCT ACTCAGCATT
    GATCGGGGGC TGGAGAGCTG CTTAAAGGGC CCAGATAATT ACAACAGCCA GGTTCTCATC
    GAAGCCACAG TCATCGCTCT CACCAAACTA CAGCCTCTCC TCGATCAGGA TTCTCCTATG
    CATAAAGCTC TGTTCTGGGT GGCCGTGGCC GTGCTTCAGT TGGATGAAGT GAATCTGTAC
    TCCGCCGGCA CTGCCTTACT GGAGCAGAAC CTGCACACAC TGGACAGCCT CCGAGTGTTT
    AATGATAAGA GTCCTGAAGA GGTTTTTATG GAGATCCGGA AACCTCTAGA GTGGCACTGT
    AAACAAATGG ACCATTTTGT GGGACTCAAC TTTGCCTCCA ACTTCAATTT TGCATTAGTG
    GGCCATTTAC TTAAAGGATA CAGACATCCC TCTTCAACAA CAGTTGCCAG GACAGTTCGT
    ATCCTGCATA CACTCCTGGG CCTGGTGGGA AAACACCGCA GCTGTGACAA GTTTGAGGTG
    AACACGCAGA GTGTGGCCTA TCTGGCAGCT TTGCTCACAG TGTCAGAGGA AGTTAGGAGC
    CGCTGCAGTT TAAAGCACAG GAAATCATTG CTGCTTTCAG AAGTTACAAT GGAGAACTTC
    CCCATGGACA CATACACCGT TTACGACACT GAACCCGGGT GCAGGCTACT GCGTGAAAAC
    CAGCCTTGGG CTTCTCCGAA AGTGTCAGAG CGCAGTCTTG CACCTCCGTA CTCCACAATA
    GGGCAGACCA GCCTTCGTCC ACGCAAATCC ATGAGTGTAG ACATGGGCCA GCCGTCTCAG
    GCCAATGCGA AGAAACTACT GGGTATTAGC GGCACTAGAA AGAGTTTCGA TCACTTAATA
    TCAGAATCCA AAGCCCCAAA AAGACCAGAC ATGGAGTCGG GCATCACCAC ACCTCCAAAG
    ATGAGGCGGG TTTCTGAAAA TGACTATGAG ATCGAAACTC AGCGAATAGC CAACTCTCAT
    TTACGCAAAG TGTCTGTGTC TGAATCCAAT GTCCTCCTGG ACGAGAACGT ACTTACGGAC
    CCCAAGATCC AGACGTTGCT CCTCACTGTA CTGGCAACAC TGGTGAAGTA CACTACAGAC
    GAGTTTCATC AGCGGATCTT GTATGAGTAT TTGGCTGAGG CCAGTGTGGT CTTCCCCAAG
    GTCTTTCCAG TTGTGCACAA CCTGCTGGAC TCCAAGTTAA GCACCGTCCT TTCCATGTGC
    CAGGACCCGA ACCTTCTGAA CCCCGTCCAT GGCATCATTC AGAACGTGGT CTATCATGAG
    GAGTCTCCCC CTCAGTACCA GCCGTCCTGC TTGCAGAGCT TTGGTTTCAA TGGTCTTTGG
    AGGTTTGCCG GGCCGTTCTC TAAGCAAACC CAAATTCCAG ACTACGCCGA GCTGGTGGTT
    AAATTTTTGG ATGCTTTGAT TGACACGTAT CTCCCGGGCA TTGAGGAGGA AGGAGGAGAA
    GAGTCACTGC GGACCCCGAC CTCGCCATAT CCACCAACCG TCCAGAGCCA GCTGAGCATC
    ACAGCCAATC TCAACCTGTC CAACTCCATG ACATCACTTG CCACATCACA GCACTCCCCA
    GGCATCGACA AGGAGAACGT TGAGATTTCT CCCACCTCGA GTCTGTCCAC CAGTGGGCGA
    ATGCGGCACG GCTCAGCAAG CCAGGTGCAG AAGCAGCGCA GCGCAGGCAG CTTCAAACGG
    CCAAGCATCA AAAAGATCGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322124.1
    CDS247..8610
    Translation

    Target ORF information:

    RefSeq Version XM_021466449.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio neurofibromin 1a (nf1a), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466449.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCGGCGC ACAAGCCCGT GGAATGGGTC CAGGCCATCA TTAACAGATT CGACGAACAG 
    CTCCCAATCA AAACAGGACA GCAGAGCACC CACACCAAAG TGAGTACAGA GCACAACAAA
    GAATGTTTGA TCAACATCTC CAAGTACAAA TTTGCCCTCG TCATCAATGG CCTGACCAAC
    ATACTCAAAA ATGTGAACAA CATGCGGATA TTTGGCGAAG CTGCTGAGAA GAACCTCTAC
    TTCTCCCAGC TGATCATACT GGACACACTG GAGAAGTGTC TAGCAGGGCA ACCTAAGGAT
    TGCCTGAGGC TGGATGAGGC TATGTTGGTA AAACAGTTGC TGCCTGAAAT CTGTCACTTC
    CTGCACTCGT ACCGTGAGGG CCAGCAACAC GCCACTCAGC TGCGCAGCTC CGCCTCTGCT
    GTGCTTTTCT CTCTCAGCTG CAACAACTTT AATGCCGTCT TCAGTCGTAT CTCCACCAGG
    TTGCAAGAGC TCACAGTTTG TACAGAGGAC ACAGTGGATG TCCATGACAT TGAGCTCATT
    CAGTATATCA ATGTAGATTG CTCCAAGTTA AAGAGACTCC TACAAGAAGC AGTACTTAAG
    TTCAAGTCTT TGAAAAAGCC GGCTCAGCTT GCAGTAATCA GCAGTTTGGA AAAGGCTTTC
    TGGAACTGGG TTGAACACTA CCCTGATGAT TTCACTATGC TCTATCAGAG ACCCCAGGCC
    GACATGGCCG ACTGTGCAGA GAGACTGTTC GACCTAGTGG ACAGTTTTGC GGAAAGCGCA
    AAGAGAAAAG CTGCTGTGTG GCCACTGCAG ATCATCCTCC TCATCCTCTG TCCAGAGATC
    ACGCAGAGCA TCACCAAAGA AGTAGGGGGA GAGGAGAAAG CTAACAAGAA GTCATTTGTA
    GACAGTTTAC GAAAGGCTCT TGGCCAACAC AGCGGCAGCA AACAATTGAC GGAAAGTGCA
    GCTGTTGCAT GCGTCAAACT CTGCAAAGCC TGCACATATA TCAACTGGGA GGATAATTCT
    GTCATCTTTC TCCTGGTTCA GTCCATCGTC ATGGACCTTA AGGGTCTCCT GTTTAACCCC
    ACCAAACCTT TCTCAAGAGG TACGGGCTGC CAGAGCGCCG ATGTAGATTT GATGATCGAC
    TGCTTTGTGT CATGTTATCG CATCAATCCT CGCAACAACC AGCATTTTAA GGTGTGTCTG
    GCCTCGACTT CTCCTCCTAC CTTCCATTTC GTCCTTGTCA ACTCCCTGCA CAAAATTATC
    ACAAATTCTC ACCTGGACTG GTGGCCGAAG ATTGACGCCA TCTACTGCTA CTCAGGAGAA
    CTACGCACCA TGTTTGCAGA CACACTGGGT CGAGTGATTC AGAGTCTCAG TGCACATCCG
    CCTCTGAGGA TGACATCAAG TTTGACATTC AAAGAGAAAA TGACAACAAG TTTGAAGTTC
    AAGGAAAAGT CCACAGATCT TGATTCGCGT TCTTTGCTGC TGGCACTGGT CAAACTTATC
    CACGCTGACC CCAAACTAAT GCTGCACAAT CCGGGAAAAC CTGGTCAGGA GATTCAGAAC
    AGCACAGCTG AGATCATCAA AGGTCTGGTT CAGCTGGTTC CTCTTACCCA CAGTGCTGAG
    CTTTCCCAAG AGGCCATGGA GGCATTGCTT GTTTTACATC AGCCAGAAAC CATTGAGCTA
    TGGAACCCGG ACGCTCCCAT TGAGACCTTC TGGGACATCA GCTCTCAGGT GTTATTTCTA
    ATTTGTAAGA AGCTGATTGG ACACCAGATG ATCAACAGTA CAGAGATACT CAAGTGGCTC
    AGGGAGATTC TCATATGCAG GAACAAGTTT CTATTAAAGA ACAAGGAGTG TGCTACACAG
    GGCAGTCTGA TCCCCATCAG CAGACAGGCT CAGACCAAGC TGGAGGTGTG TCTGTACACG
    TTCCTGTGGA GTCCTGATGC AGAGGCTGTT TTGGTGGCCA TGTCCTGTTT CAGGCATCTC
    TGTGAGGAGG CCGATATTCG CTGCAGTGTC GATGAGCTGC CTGTTCACAC AGTCCTACCC
    AATTACAACA CCTTCATTGA GCTTGCATCC GTCAGCAATA TGATGGCCAC AGGTCGCAAC
    ACACTACAAA AAAGAGTGAT GGCACTTTTA AGAAGAATAA AGCACCCTAC AGCTGGAAAT
    GTAGAGGCAT GGGAAGACAC ATATGCAAAG TGGGAGCAAG CTACAAAACA GATACTCAAC
    CACCCAAAAA ACAAAGTGGA AGATGGACAG GTAGTTAACA TAAGATTGGT CAATTATTTC
    ATCTTTTTTT CCATTCATGG TCATCTATTT TCTGTTCGTC CTCAGGAGTG GATCAACATG
    ACTGGTTTCT TGTGCGCTCT GGGAGGTGTT TGTCTCCAAC AGCGCAGCAC GCCGAGTCTG
    GGCACCTACA GTCCTCCAAT GGGCCCCCTG ACCGAACGCA AGGGGTCCAT GATTTCCATG
    AGCTCCTGCG AGGTAAACAG CGAAACATCC CTGAATCGCT TTCTGGAGCG TCTGCTGGCT
    CTGATGGTCT GCACACATGA GAAAGTGGGA GTGCAAATTC GCACCAACGT CAAAGACCTG
    GTGGGACTGG AGCTCAGTCC GGCTCTCTAT TCAATGCTCT TCACTAAGCT GCGCAACAGC
    ATCGGACGCT TCTTTGACAC CCAGGGGCCG GTTCCTATTA CAGATGCAAA CACTCTGTTT
    GTAGAGCAGA CCATTGCTAT CATGAAGAAC TTGCTGGACA GTCACACTGA AGGAAGCTCA
    GAGCACCTTG GTCAAGCCAG CATTGAGACC ATGATGCTCA ATCTAGTTAG GTATGTGCGT
    ATTTTAGGGA ACATTGTTCA TGCCATTCAG ATCAAAACCA AGTTGTGTCA GTTAGTGGAG
    GTGATGATGG AGCGCAGGGA TGACCTCTCC TTCTGCCAGG AGATGAAATT CAGGAATAAA
    ATGGTGGAGT ACCTCACAGA CTGGGTGATG GGAACCTCCA ATCAAGCTGC GGACGATGAT
    GTCAAATGCC TGACCCGCGA TCTGGACCAG GCCAGTATGG AGGCTGTCGT GTCTCTGTTG
    GCTGGTCTTC CTCTACAGCC TGAGGAGGGT GACGGTGTTG AGCTCATGGA GGCCAAATCA
    CAGCTCTTCC TGAAGTACTT CACACTGTTC ATGAACCTGT TGAATGACTG CAGCGAGGTT
    GAGGAAGATG GCCAGCAGGT CGCAGGGAGG AAACGTGGCA TGTCTAGACG ACTGGCATCT
    CTGCGGCACT GCACAGTATT GGCTATGTCT AACTTGCTCA ATGCTAATGT GGATAGCGGC
    CTCATGCACT CAATCGGTCT GGGATACCAT AAGGACCTGC AGACACGGGC TACATTTATG
    GAAGTGCTCA CTAAGATCCT TCAGCAAGGG ACGGAGTTCG ACACGCTTGC AGAGACTGTG
    CTTGCAGATC GCTTTGAGAG ACTGGTCGAG CTGGTCACCA TGATGGGAGA TCAGGGAGAG
    CTGCCCATAG CCATGGCTCT GGCCAATGTG GTCCCATGCT CTCAATGGGA TGAGCTGGCC
    AGAGTGTTAG TGACTCTCTT CGACTCTCGC CATCTGCTGT ATCAGCTGTT GTGGAATATG
    TTCTCCAAAG AGGTGGAGCT GGCAGACTCA ATGCAGACGC TCTTTAGAGG AAATAGCCTA
    GCCAGCAAGA TTATGACCTT CTGCTTCAAG GTGTACGGAG CTGCGTATCT ACAGAAGCTT
    CTGGAGCCGC TGTTGAGAGG AATCATCACT ACACCAGAGT GGCAGCAGAT CAGTTTTGAA
    GTGGACCCTA CTAGACTTGA ACAGGCAGAG AACCTGGAGG AGAACCAGAA GAACCTGCTT
    CAGATCACTG ACCGCTTCTT CCTGGCCATC ATCAACTCCT CGAGCGAGTT TCCTCCACAG
    CTGCGCAGTG TGTGCCACTG TCTGTATCAG GCTACTTGCC ACTCCCTTCT GAATAAAGCC
    ACTGTTAAAG AAAAAAAGGA AAACAAAAAA TCCGTGGTCA GTCAGCGGTT TCCTCAGAAC
    AGCATTGGTG CTGTAGGAAG TGCCATGTTC CTGCGCTTTA TTAACCCAGC TATCGTGTCC
    CCATATGAGG CTGGAATCCT AGACAAGAAA CCCCCACCTC GCATCGAGCG TGGCCTTAAA
    CTCATGTCTA AGATTCTGCA GAGCATTGCT AATCATGTTC TCTTCACAAA AGAGGAGCAT
    ATGAGGCCCT TTAATGATTT TGTCAAAAGC AACTTCGATG CAGCCAGAAG GTTCTTCTTG
    GATATCGCAT CTGATTCTCC ACCAAGTGAT TCTGTGAACC ATAGTCTGTC TTTCATCAGT
    GACGGTAATG TGCTCGCTCT GCACCGGCTA TTATGGAACA ACCAGGAAAG AATTGGCCAA
    TACCTGTCCA GCAACAGGGA CCACAAGGCA GTGGGCCGTA GACCATTTGA CAAGATGGCC
    ACTCTTCTGG CGTACCTGGG TCCTCCTGAA CATAAGCCAG TAGCAGACAC ACACTGGTCC
    AGCCTCAACC TCACCAGCTC AAAGTTTGAG GAGTTCATGA CAAGGCATCA AGTCCATGAG
    AAGGAGGAAT TTAAAGCATT AAAAACCCTC AACATATTTT ACCAAGCTGG CACATCTAAG
    AACGGCAACC CTGTGTTTTA TTACATCGCA CGCAGGTTCA AAACTGGACA AATCAATGGT
    GATCTTCTGA TCTACCACGT CCTGCTGACG CTCAAGCCCT ACTATGCCAA GCATTATGAG
    ATCGTAGTTG ACCTTACTCA TGTGGGCCCC AGTAACCGTT TCAAGACGGA CTTCTTATCC
    AAATGGTTTG TGGTCTTTCC AAACTTCGCC TATGAGAACG TGGCTGCTGT GTACATCTAC
    AACTGCAACA CCTGGGTACG CGAGTACACC AAATACCACG AGCGACTGCT GACTGGCCTC
    AAGGGGAGCA AGAAGCTGGT GTTTATCGAT TCGCCTGCAC GTCTAGCTGA ACACATCGAA
    CCCGAACAGC AGAAGCTGCC CGCAGCTACT TTAGCACTGG AAGAAGACCT CAAAGTGTTC
    CACAACGCCC TAAAGCTTGC ACACAGAGAC ACAAAAGTGT CAATTAAGGT GGGATCCACA
    GCTGTACAGG TGACCTCGGC CGAAAGAACG CGTGTCCTAG GACAGCCTGT GTTCCTAAAT
    GACATCTACT ACGCTTCAGA GATCGAGGAG ATATGCCTGG TGGACGAGAA CCAGTTCACG
    CTAACCATCG CTAACCAGGG CACCCCACTC ACTTTCATGC ACCAGGAGTG TGAAGCCATC
    GTTCAGTCCA TCATCCACAT CCGCACGCGC TGGGAACTTT CCCAACCAGA CTCCATTCCC
    CAACACACCA AAATCAGGCC TAAGGATGTT CCTGGCACCC TGCTCAACAT CGCTCTTCTC
    AACCTGGGAA GCTCCGACCC CAGTCTGAGG TCTGCGGCCT ACAATCTCCT TTGTGCCTTA
    ACCTGCACCT TCAACCTGAA GATTGAAGGC CAGCTTCTGG AAACGTCTGG CTTGTGCATC
    CCAGCCAACA ACACGCTTTT TATTGTGTCC ATTAGCAAAA CGCTAGCAGC TAATGAACCC
    CACCTCACTC TGGAGTTCCT GGAAGAGTGC ATCTCAGGAT TCAGTAAATC AAGTATAGAG
    CTGAAGCATC TGTGTCTAGA ATATATGACT CCTTGGCTCC GTAATTTGGT GCGCTTTTGC
    AAACACAATG ATGACGCCAA ACGGCAGCGT GTCACGGCCA TCCTGGACAA GCTTATTACT
    ATGACCATCA ACGAGAAGCA GATGTACCCC TCCATACAGG CCAAGATATG GGGCAGCTTG
    GGTCAGATCA CAGACCTGCT TGATGTGGTG TTGGACAGCT TTATAAAGAC CAGCGCTACT
    GGCGGTTTAG GATCCATCAA AGCTGAAGTG ATGGCTGACA CTGCTGTAGC TTTGGCGTCA
    GGAAATGTCA AACTGGTCTC CAGCAAGGTG ATTGGCCGAA TGTGTAAGAT CATCGACAAG
    ACGTGTCTGT CTCCCACTCC GACCCTTGAG CAGCACCTCA TGTGGGACGA CATCGCCATC
    CTGGCCCGGT ACATGCTCAT GCTGTCCTTC AACAACTCTC TGGACGTGGC CGCTCATCTG
    CCATACCTGT TCCACGTCGT CACCCTGCTG GTGGCCACGG GCCCTCTCTC GCTGCGTGCA
    TCAACACACG GCCTGGTCAT CAACATCATT CACTCGCTCT GCACATGCTC ACAACTCAAC
    TTCAGTGAGG AGACTAAACA GGTGCTGCGC CTGAGTCTGA CTGAGTTCTC CCTGCCCAAG
    TTCTACCTGC TGTTCGGCAT CAGTAAGGTC AAATCTGCGG CGGTCATCGC TTTCCGTTCC
    AGCTACAGAG ACCGCTCATT CTCTCCAGGC TCTTATGAGA GAGAGACATT TGCTCTGTCT
    TCCCTGGAAA CAGTCACGGA GGCTCTTTTG GAGATCATGG AGGCCTGCAT GAGGGACATT
    CCTGGATGCA AATGGCTAGA TCAGTGGACT GAATTGGCGC AAAAGTTTGC GTTCCAGTAT
    AACCCCTCTC TTCAGCCTCG GGCGCTGGTG GTGTTCGGCT GCATCAGTAA ACGAGTGACT
    CACGGACAGC TAAAGCAGAT CATTCGTATT CTCAGTAAAG CCAGTCCTCT ACTCAGCATT
    GATCGGGGGC TGGAGAGCTG CTTAAAGGGC CCAGATAATT ACAACAGCCA GGTTCTCATC
    GAAGCCACAG TCATCGCTCT CACCAAACTA CAGCCTCTCC TCGATCAGGA TTCTCCTATG
    CATAAAGCTC TGTTCTGGGT GGCCGTGGCC GTGCTTCAGT TGGATGAAGT GAATCTGTAC
    TCCGCCGGCA CTGCCTTACT GGAGCAGAAC CTGCACACAC TGGACAGCCT CCGAGTGTTT
    AATGATAAGA GTCCTGAAGA GGTTTTTATG GAGATCCGGA AACCTCTAGA GTGGCACTGT
    AAACAAATGG ACCATTTTGT GGGACTCAAC TTTGCCTCCA ACTTCAATTT TGCATTAGTG
    GGCCATTTAC TTAAAGGATA CAGACATCCC TCTTCAACAA CAGTTGCCAG GACAGTTCGT
    ATCCTGCATA CACTCCTGGG CCTGGTGGGA AAACACCGCA GCTGTGACAA GTTTGAGGTG
    AACACGCAGA GTGTGGCCTA TCTGGCAGCT TTGCTCACAG TGTCAGAGGA AGTTAGGAGC
    CGCTGCAGTT TAAAGCACAG GAAATCATTG CTGCTTTCAG AAGTTACAAT GGAGAACTTC
    CCCATGGACA CATACACCGT TTACGACACT GAACCCGGGT GCAGGCTACT GCGTGAAAAC
    CAGCCTTGGG CTTCTCCGAA AGTGTCAGAG CGCAGTCTTG CACCTCCGTA CTCCACAATA
    GGGCAGACCA GCCTTCGTCC ACGCAAATCC ATGAGTGTAG ACATGGGCCA GCCGTCTCAG
    GCCAATGCGA AGAAACTACT GGGTATTAGC GGCACTAGAA AGAGTTTCGA TCACTTAATA
    TCAGAATCCA AAGCCCCAAA AAGACCAGAC ATGGAGTCGG GCATCACCAC ACCTCCAAAG
    ATGAGGCGGG TTTCTGAAAA TGACTATGAG ATCGAAACTC AGCGAATAGC CAACTCTCAT
    TTACGCAAAG TGTCTGTGTC TGAATCCAAT GTCCTCCTGG ACGAGAACGT ACTTACGGAC
    CCCAAGATCC AGACGTTGCT CCTCACTGTA CTGGCAACAC TGGTGAAGTA CACTACAGAC
    GAGTTTCATC AGCGGATCTT GTATGAGTAT TTGGCTGAGG CCAGTGTGGT CTTCCCCAAG
    GTCTTTCCAG TTGTGCACAA CCTGCTGGAC TCCAAGTTAA GCACCGTCCT TTCCATGTGC
    CAGGACCCGA ACCTTCTGAA CCCCGTCCAT GGCATCATTC AGAACGTGGT CTATCATGAG
    GAGTCTCCCC CTCAGTACCA GCCGTCCTGC TTGCAGAGCT TTGGTTTCAA TGGTCTTTGG
    AGGTTTGCCG GGCCGTTCTC TAAGCAAACC CAAATTCCAG ACTACGCCGA GCTGGTGGTT
    AAATTTTTGG ATGCTTTGAT TGACACGTAT CTCCCGGGCA TTGAGGAGGA AGGAGGAGAA
    GAGTCACTGC GGACCCCGAC CTCGCCATAT CCACCAACCG TCCAGAGCCA GCTGAGCATC
    ACAGCCAATC TCAACCTGTC CAACTCCATG ACATCACTTG CCACATCACA GCACTCCCCA
    GGCATCGACA AGGAGAACGT TGAGATTTCT CCCACCTCGA GTCTGTCCAC CAGTGGGCGA
    ATGCGGCACG GCTCAGCAAG CCAGGTGCAG AAGCAGCGCA GCGCAGGCAG CTTCAAACGG
    CCAAGCATCA AAAAGATCGT CTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa39877
    Clone ID Related Accession (Same CDS sequence) XM_021466450.1
    Accession Version XM_021466450.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8355bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product neurofibromin isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCGGCGC ACAAGCCCGT GGAATGGGTC CAGGCCATCA TTAACAGATT CGACGAACAG 
    CTCCCAATCA AAACAGGACA GCAGAGCACC CACACCAAAG TGAGTACAGA GCACAACAAA
    GAATGTTTGA TCAACATCTC CAAGTACAAA TTTGCCCTCG TCATCAATGG CCTGACCAAC
    ATACTCAAAA ATGTGAACAA CATGCGGATA TTTGGCGAAG CTGCTGAGAA GAACCTCTAC
    TTCTCCCAGC TGATCATACT GGACACACTG GAGAAGTGTC TAGCAGGGCA ACCTAAGGAT
    TGCCTGAGGC TGGATGAGGC TATGTTGGTA AAACAGTTGC TGCCTGAAAT CTGTCACTTC
    CTGCACTCGT ACCGTGAGGG CCAGCAACAC GCCACTCAGC TGCGCAGCTC CGCCTCTGCT
    GTGCTTTTCT CTCTCAGCTG CAACAACTTT AATGCCGTCT TCAGTCGTAT CTCCACCAGG
    TTGCAAGAGC TCACAGTTTG TACAGAGGAC ACAGTGGATG TCCATGACAT TGAGCTCATT
    CAGTATATCA ATGTAGATTG CTCCAAGTTA AAGAGACTCC TACAAGAAGC AGTACTTAAG
    TTCAAGTCTT TGAAAAAGCC GGCTCAGCTT GCAGTAATCA GCAGTTTGGA AAAGGCTTTC
    TGGAACTGGG TTGAACACTA CCCTGATGAT TTCACTATGC TCTATCAGAG ACCCCAGGCC
    GACATGGCCG ACTGTGCAGA GAGACTGTTC GACCTAGTGG ACAGTTTTGC GGAAAGCGCA
    AAGAGAAAAG CTGCTGTGTG GCCACTGCAG ATCATCCTCC TCATCCTCTG TCCAGAGATC
    ACGCAGAGCA TCACCAAAGA AGTAGGGGGA GAGGAGAAAG CTAACAAGAA GTCATTTGTA
    GACAGTTTAC GAAAGGCTCT TGGCCAACAC AGCGGCAGCA AACAATTGAC GGAAAGTGCA
    GCTGTTGCAT GCGTCAAACT CTGCAAAGCC TGCACATATA TCAACTGGGA GGATAATTCT
    GTCATCTTTC TCCTGGTTCA GTCCATCGTC ATGGACCTTA AGGGTCTCCT GTTTAACCCC
    ACCAAACCTT TCTCAAGAGG TACGGGCTGC CAGAGCGCCG ATGTAGATTT GATGATCGAC
    TGCTTTGTGT CATGTTATCG CATCAATCCT CGCAACAACC AGCATTTTAA GGTGTGTCTG
    GCCTCGACTT CTCCTCCTAC CTTCCATTTC GTCCTTGTCA ACTCCCTGCA CAAAATTATC
    ACAAATTCTC ACCTGGACTG GTGGCCGAAG ATTGACGCCA TCTACTGCTA CTCAGGAGAA
    CTACGCACCA TGTTTGCAGA CACACTGGGT CGAGTGATTC AGAGTCTCAG TGCACATCCG
    CCTCTGAGGA TGACATCAAG TTTGACATTC AAAGAGAAAA TGACAACAAG TTTGAAGTTC
    AAGGAAAAGT CCACAGATCT TGATTCGCGT TCTTTGCTGC TGGCACTGGT CAAACTTATC
    CACGCTGACC CCAAACTAAT GCTGCACAAT CCGGGAAAAC CTGGTCAGGA GATTCAGAAC
    AGCACAGCTG AGATCATCAA AGGTCTGGTT CAGCTGGTTC CTCTTACCCA CAGTGCTGAG
    CTTTCCCAAG AGGCCATGGA GGCATTGCTT GTTTTACATC AGCCAGAAAC CATTGAGCTA
    TGGAACCCGG ACGCTCCCAT TGAGACCTTC TGGGACATCA GCTCTCAGGT GTTATTTCTA
    ATTTGTAAGA AGCTGATTGG ACACCAGATG ATCAACAGTA CAGAGATACT CAAGTGGCTC
    AGGGAGATTC TCATATGCAG GAACAAGTTT CTATTAAAGA ACAAGGAGTG TGCTACACAG
    GGCAGTCTGA TCCCCATCAG CAGACAGGCT CAGACCAAGC TGGAGGTGTG TCTGTACACG
    TTCCTGTGGA GTCCTGATGC AGAGGCTGTT TTGGTGGCCA TGTCCTGTTT CAGGCATCTC
    TGTGAGGAGG CCGATATTCG CTGCAGTGTC GATGAGCTGC CTGTTCACAC AGTCCTACCC
    AATTACAACA CCTTCATTGA GCTTGCATCC GTCAGCAATA TGATGGCCAC AGGTCGCAAC
    ACACTACAAA AAAGAGTGAT GGCACTTTTA AGAAGAATAA AGCACCCTAC AGCTGGAAAT
    GTAGAGGCAT GGGAAGACAC ATATGCAAAG TGGGAGCAAG CTACAAAACA GATACTCAAC
    CACCCAAAAA ACAAAGTGGA AGATGGACAG GTAGTTAACA TAAGATTGGT CAATTATTTC
    ATCTTTTTTT CCATTCATGG TCATCTATTT TCTGTTCGTC CTCAGGAGTG GATCAACATG
    ACTGGTTTCT TGTGCGCTCT GGGAGGTGTT TGTCTCCAAC AGCGCAGCAC GCCGAGTCTG
    GGCACCTACA GTCCTCCAAT GGGCCCCCTG ACCGAACGCA AGGGGTCCAT GATTTCCATG
    AGCTCCTGCG AGGTAAACAG CGAAACATCC CTGAATCGCT TTCTGGAGCG TCTGCTGGCT
    CTGATGGTCT GCACACATGA GAAAGTGGGA GTGCAAATTC GCACCAACGT CAAAGACCTG
    GTGGGACTGG AGCTCAGTCC GGCTCTCTAT TCAATGCTCT TCACTAAGCT GCGCAACAGC
    ATCGGACGCT TCTTTGACAC CCAGGGGCCG GTTCCTATTA CAGATGCAAA CACTCTGTTT
    GTAGAGCAGA CCATTGCTAT CATGAAGAAC TTGCTGGACA GTCACACTGA AGGAAGCTCA
    GAGCACCTTG GTCAAGCCAG CATTGAGACC ATGATGCTCA ATCTAGTTAG GTATGTGCGT
    ATTTTAGGGA ACATTGTTCA TGCCATTCAG ATCAAAACCA AGTTGTGTCA GTTAGTGGAG
    GTGATGATGG AGCGCAGGGA TGACCTCTCC TTCTGCCAGG AGATGAAATT CAGGAATAAA
    ATGGTGGAGT ACCTCACAGA CTGGGTGATG GGAACCTCCA ATCAAGCTGC GGACGATGAT
    GTCAAATGCC TGACCCGCGA TCTGGACCAG GCCAGTATGG AGGCTGTCGT GTCTCTGTTG
    GCTGGTCTTC CTCTACAGCC TGAGGAGGGT GACGGTGTTG AGCTCATGGA GGCCAAATCA
    CAGCTCTTCC TGAAGTACTT CACACTGTTC ATGAACCTGT TGAATGACTG CAGCGAGGTT
    GAGGAAGATG GCCAGCAGGT CGCAGGGAGG AAACGTGGCA TGTCTAGACG ACTGGCATCT
    CTGCGGCACT GCACAGTATT GGCTATGTCT AACTTGCTCA ATGCTAATGT GGATAGCGGC
    CTCATGCACT CAATCGGTCT GGGATACCAT AAGGACCTGC AGACACGGGC TACATTTATG
    GAAGTGCTCA CTAAGATCCT TCAGCAAGGG ACGGAGTTCG ACACGCTTGC AGAGACTGTG
    CTTGCAGATC GCTTTGAGAG ACTGGTCGAG CTGGTCACCA TGATGGGAGA TCAGGGAGAG
    CTGCCCATAG CCATGGCTCT GGCCAATGTG GTCCCATGCT CTCAATGGGA TGAGCTGGCC
    AGAGTGTTAG TGACTCTCTT CGACTCTCGC CATCTGCTGT ATCAGCTGTT GTGGAATATG
    TTCTCCAAAG AGGTGGAGCT GGCAGACTCA ATGCAGACGC TCTTTAGAGG AAATAGCCTA
    GCCAGCAAGA TTATGACCTT CTGCTTCAAG GTGTACGGAG CTGCGTATCT ACAGAAGCTT
    CTGGAGCCGC TGTTGAGAGG AATCATCACT ACACCAGAGT GGCAGCAGAT CAGTTTTGAA
    GTGGACCCTA CTAGACTTGA ACAGGCAGAG AACCTGGAGG AGAACCAGAA GAACCTGCTT
    CAGATCACTG ACCGCTTCTT CCTGGCCATC ATCAACTCCT CGAGCGAGTT TCCTCCACAG
    CTGCGCAGTG TGTGCCACTG TCTGTATCAG GCTACTTGCC ACTCCCTTCT GAATAAAGCC
    ACTGTTAAAG AAAAAAAGGA AAACAAAAAA TCCGTGGTCA GTCAGCGGTT TCCTCAGAAC
    AGCATTGGTG CTGTAGGAAG TGCCATGTTC CTGCGCTTTA TTAACCCAGC TATCGTGTCC
    CCATATGAGG CTGGAATCCT AGACAAGAAA CCCCCACCTC GCATCGAGCG TGGCCTTAAA
    CTCATGTCTA AGATTCTGCA GAGCATTGCT AATCATGTTC TCTTCACAAA AGAGGAGCAT
    ATGAGGCCCT TTAATGATTT TGTCAAAAGC AACTTCGATG CAGCCAGAAG GTTCTTCTTG
    GATATCGCAT CTGATTCTCC ACCAAGTGAT TCTGTGAACC ATAGTCTGTC TTTCATCAGT
    GACGGTAATG TGCTCGCTCT GCACCGGCTA TTATGGAACA ACCAGGAAAG AATTGGCCAA
    TACCTGTCCA GCAACAGGGA CCACAAGGCA GTGGGCCGTA GACCATTTGA CAAGATGGCC
    ACTCTTCTGG CGTACCTGGG TCCTCCTGAA CATAAGCCAG TAGCAGACAC ACACTGGTCC
    AGCCTCAACC TCACCAGCTC AAAGTTTGAG GAGTTCATGA CAAGGCATCA AGTCCATGAG
    AAGGAGGAAT TTAAAGCATT AAAAACCCTC AACATATTTT ACCAAGCTGG CACATCTAAG
    AACGGCAACC CTGTGTTTTA TTACATCGCA CGCAGGTTCA AAACTGGACA AATCAATGGT
    GATCTTCTGA TCTACCACGT CCTGCTGACG CTCAAGCCCT ACTATGCCAA GCATTATGAG
    ATCGTAGTTG ACCTTACTCA TGTGGGCCCC AGTAACCGTT TCAAGACGGA CTTCTTATCC
    AAATGGTTTG TGGTCTTTCC AAACTTCGCC TATGAGAACG TGGCTGCTGT GTACATCTAC
    AACTGCAACA CCTGGGTACG CGAGTACACC AAATACCACG AGCGACTGCT GACTGGCCTC
    AAGGGGAGCA AGAAGCTGGT GTTTATCGAT TCGCCTGCAC GTCTAGCTGA ACACATCGAA
    CCCGAACAGC AGAAGCTGCC CGCAGCTACT TTAGCACTGG AAGAAGACCT CAAAGTGTTC
    CACAACGCCC TAAAGCTTGC ACACAGAGAC ACAAAAGTGT CAATTAAGGT GGGATCCACA
    GCTGTACAGG TGACCTCGGC CGAAAGAACG CGTGTCCTAG GACAGCCTGT GTTCCTAAAT
    GACATCTACT ACGCTTCAGA GATCGAGGAG ATATGCCTGG TGGACGAGAA CCAGTTCACG
    CTAACCATCG CTAACCAGGG CACCCCACTC ACTTTCATGC ACCAGGAGTG TGAAGCCATC
    GTTCAGTCCA TCATCCACAT CCGCACGCGC TGGGAACTTT CCCAACCAGA CTCCATTCCC
    CAACACACCA AAATCAGGCC TAAGGATGTT CCTGGCACCC TGCTCAACAT CGCTCTTCTC
    AACCTGGGAA GCTCCGACCC CAGTCTGAGG TCTGCGGCCT ACAATCTCCT TTGTGCCTTA
    ACCTGCACCT TCAACCTGAA GATTGAAGGC CAGCTTCTGG AAACGTCTGG CTTGTGCATC
    CCAGCCAACA ACACGCTTTT TATTGTGTCC ATTAGCAAAA CGCTAGCAGC TAATGAACCC
    CACCTCACTC TGGAGTTCCT GGAAGAGTGC ATCTCAGGAT TCAGTAAATC AAGTATAGAG
    CTGAAGCATC TGTGTCTAGA ATATATGACT CCTTGGCTCC GTAATTTGGT GCGCTTTTGC
    AAACACAATG ATGACGCCAA ACGGCAGCGT GTCACGGCCA TCCTGGACAA GCTTATTACT
    ATGACCATCA ACGAGAAGCA GATGTACCCC TCCATACAGG CCAAGATATG GGGCAGCTTG
    GGTCAGATCA CAGACCTGCT TGATGTGGTG TTGGACAGCT TTATAAAGAC CAGCGCTACT
    GGCGGTTTAG GATCCATCAA AGCTGAAGTG ATGGCTGACA CTGCTGTAGC TTTGGCGTCA
    GGAAATGTCA AACTGGTCTC CAGCAAGGTG ATTGGCCGAA TGTGTAAGAT CATCGACAAG
    ACGTGTCTGT CTCCCACTCC GACCCTTGAG CAGCACCTCA TGTGGGACGA CATCGCCATC
    CTGGCCCGGT ACATGCTCAT GCTGTCCTTC AACAACTCTC TGGACGTGGC CGCTCATCTG
    CCATACCTGT TCCACGTCGT CACCCTGCTG GTGGCCACGG GCCCTCTCTC GCTGCGTGCA
    TCAACACACG GCCTGGTCAT CAACATCATT CACTCGCTCT GCACATGCTC ACAACTCAAC
    TTCAGTGAGG AGACTAAACA GGTGCTGCGC CTGAGTCTGA CTGAGTTCTC CCTGCCCAAG
    TTCTACCTGC TGTTCGGCAT CAGTAAGGTC AAATCTGCGG CGGTCATCGC TTTCCGTTCC
    AGCTACAGAG ACCGCTCATT CTCTCCAGGC TCTTATGAGA GAGAGACATT TGCTCTGTCT
    TCCCTGGAAA CAGTCACGGA GGCTCTTTTG GAGATCATGG AGGCCTGCAT GAGGGACATT
    CCTGGATGCA AATGGCTAGA TCAGTGGACT GAATTGGCGC AAAAGTTTGC GTTCCAGTAT
    AACCCCTCTC TTCAGCCTCG GGCGCTGGTG GTGTTCGGCT GCATCAGTAA ACGAGTGACT
    CACGGACAGC TAAAGCAGAT CATTCGTATT CTCAGTAAAG CCAGTCCTCT ACTCAGCATT
    GATCGGGGGC TGGAGAGCTG CTTAAAGGGC CCAGATAATT ACAACAGCCA GGTTCTCATC
    GAAGCCACAG TCATCGCTCT CACCAAACTA CAGCCTCTCC TCGATCAGGA TTCTCCTATG
    CATAAAGCTC TGTTCTGGGT GGCCGTGGCC GTGCTTCAGT TGGATGAAGT GAATCTGTAC
    TCCGCCGGCA CTGCCTTACT GGAGCAGAAC CTGCACACAC TGGACAGCCT CCGAGTGTTT
    AATGATAAGA GTCCTGAAGA GGTTTTTATG GAGATCCGGA AACCTCTAGA GTGGCACTGT
    AAACAAATGG ACCATTTTGT GGGACTCAAC TTTGCCTCCA ACTTCAATTT TGCATTAGTG
    GGCCATTTAC TTAAAGGATA CAGACATCCC TCTTCAACAA CAGTTGCCAG GACAGTTCGT
    ATCCTGCATA CACTCCTGGG CCTGGTGGGA AAACACCGCA GCTGTGACAA GTTTGAGGTG
    AACACGCAGA GTGTGGCCTA TCTGGCAGCT TTGCTCACAG TGTCAGAGGA AGTTAGGAGC
    CGCTGCAGTT TAAAGCACAG GAAATCATTG CTGCTTTCAG AAGTTACAAT GGAGAACTTC
    CCCATGGACA CATACACCGT TTACGACACT GAACCCGGGT GCAGGCTACT GCGTGAAAAC
    CAGCCTTGGG CTTCTCCGAA AGTGTCAGAG CGCAGTCTTG CACCTCCGTA CTCCACAATA
    GGGCAGACCA GCCTTCGTCC ACGCAAATCC ATGAGTGTAG ACATGGGCCA GCCGTCTCAG
    GCCAATGCGA AGAAACTACT GGGCACTAGA AAGAGTTTCG ATCACTTAAT ATCAGAATCC
    AAAGCCCCAA AAAGACCAGA CATGGAGTCG GGCATCACCA CACCTCCAAA GATGAGGCGG
    GTTTCTGAAA ATGACTATGA GATCGAAACT CAGCGAATAG CCAACTCTCA TTTACGCAAA
    GTGTCTGTGT CTGAATCCAA TGTCCTCCTG GACGAGAACG TACTTACGGA CCCCAAGATC
    CAGACGTTGC TCCTCACTGT ACTGGCAACA CTGGTGAAGT ACACTACAGA CGAGTTTCAT
    CAGCGGATCT TGTATGAGTA TTTGGCTGAG GCCAGTGTGG TCTTCCCCAA GGTCTTTCCA
    GTTGTGCACA ACCTGCTGGA CTCCAAGTTA AGCACCGTCC TTTCCATGTG CCAGGACCCG
    AACCTTCTGA ACCCCGTCCA TGGCATCATT CAGAACGTGG TCTATCATGA GGAGTCTCCC
    CCTCAGTACC AGCCGTCCTG CTTGCAGAGC TTTGGTTTCA ATGGTCTTTG GAGGTTTGCC
    GGGCCGTTCT CTAAGCAAAC CCAAATTCCA GACTACGCCG AGCTGGTGGT TAAATTTTTG
    GATGCTTTGA TTGACACGTA TCTCCCGGGC ATTGAGGAGG AAGGAGGAGA AGAGTCACTG
    CGGACCCCGA CCTCGCCATA TCCACCAACC GTCCAGAGCC AGCTGAGCAT CACAGCCAAT
    CTCAACCTGT CCAACTCCAT GACATCACTT GCCACATCAC AGCACTCCCC AGGCATCGAC
    AAGGAGAACG TTGAGATTTC TCCCACCTCG AGTCTGTCCA CCAGTGGGCG AATGCGGCAC
    GGCTCAGCAA GCCAGGTGCA GAAGCAGCGC AGCGCAGGCA GCTTCAAACG GCCAAGCATC
    AAAAAGATCG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021322125.1
    CDS247..8601
    Translation

    Target ORF information:

    RefSeq Version XM_021466450.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio neurofibromin 1a (nf1a), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021466450.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCGGCGC ACAAGCCCGT GGAATGGGTC CAGGCCATCA TTAACAGATT CGACGAACAG 
    CTCCCAATCA AAACAGGACA GCAGAGCACC CACACCAAAG TGAGTACAGA GCACAACAAA
    GAATGTTTGA TCAACATCTC CAAGTACAAA TTTGCCCTCG TCATCAATGG CCTGACCAAC
    ATACTCAAAA ATGTGAACAA CATGCGGATA TTTGGCGAAG CTGCTGAGAA GAACCTCTAC
    TTCTCCCAGC TGATCATACT GGACACACTG GAGAAGTGTC TAGCAGGGCA ACCTAAGGAT
    TGCCTGAGGC TGGATGAGGC TATGTTGGTA AAACAGTTGC TGCCTGAAAT CTGTCACTTC
    CTGCACTCGT ACCGTGAGGG CCAGCAACAC GCCACTCAGC TGCGCAGCTC CGCCTCTGCT
    GTGCTTTTCT CTCTCAGCTG CAACAACTTT AATGCCGTCT TCAGTCGTAT CTCCACCAGG
    TTGCAAGAGC TCACAGTTTG TACAGAGGAC ACAGTGGATG TCCATGACAT TGAGCTCATT
    CAGTATATCA ATGTAGATTG CTCCAAGTTA AAGAGACTCC TACAAGAAGC AGTACTTAAG
    TTCAAGTCTT TGAAAAAGCC GGCTCAGCTT GCAGTAATCA GCAGTTTGGA AAAGGCTTTC
    TGGAACTGGG TTGAACACTA CCCTGATGAT TTCACTATGC TCTATCAGAG ACCCCAGGCC
    GACATGGCCG ACTGTGCAGA GAGACTGTTC GACCTAGTGG ACAGTTTTGC GGAAAGCGCA
    AAGAGAAAAG CTGCTGTGTG GCCACTGCAG ATCATCCTCC TCATCCTCTG TCCAGAGATC
    ACGCAGAGCA TCACCAAAGA AGTAGGGGGA GAGGAGAAAG CTAACAAGAA GTCATTTGTA
    GACAGTTTAC GAAAGGCTCT TGGCCAACAC AGCGGCAGCA AACAATTGAC GGAAAGTGCA
    GCTGTTGCAT GCGTCAAACT CTGCAAAGCC TGCACATATA TCAACTGGGA GGATAATTCT
    GTCATCTTTC TCCTGGTTCA GTCCATCGTC ATGGACCTTA AGGGTCTCCT GTTTAACCCC
    ACCAAACCTT TCTCAAGAGG TACGGGCTGC CAGAGCGCCG ATGTAGATTT GATGATCGAC
    TGCTTTGTGT CATGTTATCG CATCAATCCT CGCAACAACC AGCATTTTAA GGTGTGTCTG
    GCCTCGACTT CTCCTCCTAC CTTCCATTTC GTCCTTGTCA ACTCCCTGCA CAAAATTATC
    ACAAATTCTC ACCTGGACTG GTGGCCGAAG ATTGACGCCA TCTACTGCTA CTCAGGAGAA
    CTACGCACCA TGTTTGCAGA CACACTGGGT CGAGTGATTC AGAGTCTCAG TGCACATCCG
    CCTCTGAGGA TGACATCAAG TTTGACATTC AAAGAGAAAA TGACAACAAG TTTGAAGTTC
    AAGGAAAAGT CCACAGATCT TGATTCGCGT TCTTTGCTGC TGGCACTGGT CAAACTTATC
    CACGCTGACC CCAAACTAAT GCTGCACAAT CCGGGAAAAC CTGGTCAGGA GATTCAGAAC
    AGCACAGCTG AGATCATCAA AGGTCTGGTT CAGCTGGTTC CTCTTACCCA CAGTGCTGAG
    CTTTCCCAAG AGGCCATGGA GGCATTGCTT GTTTTACATC AGCCAGAAAC CATTGAGCTA
    TGGAACCCGG ACGCTCCCAT TGAGACCTTC TGGGACATCA GCTCTCAGGT GTTATTTCTA
    ATTTGTAAGA AGCTGATTGG ACACCAGATG ATCAACAGTA CAGAGATACT CAAGTGGCTC
    AGGGAGATTC TCATATGCAG GAACAAGTTT CTATTAAAGA ACAAGGAGTG TGCTACACAG
    GGCAGTCTGA TCCCCATCAG CAGACAGGCT CAGACCAAGC TGGAGGTGTG TCTGTACACG
    TTCCTGTGGA GTCCTGATGC AGAGGCTGTT TTGGTGGCCA TGTCCTGTTT CAGGCATCTC
    TGTGAGGAGG CCGATATTCG CTGCAGTGTC GATGAGCTGC CTGTTCACAC AGTCCTACCC
    AATTACAACA CCTTCATTGA GCTTGCATCC GTCAGCAATA TGATGGCCAC AGGTCGCAAC
    ACACTACAAA AAAGAGTGAT GGCACTTTTA AGAAGAATAA AGCACCCTAC AGCTGGAAAT
    GTAGAGGCAT GGGAAGACAC ATATGCAAAG TGGGAGCAAG CTACAAAACA GATACTCAAC
    CACCCAAAAA ACAAAGTGGA AGATGGACAG GTAGTTAACA TAAGATTGGT CAATTATTTC
    ATCTTTTTTT CCATTCATGG TCATCTATTT TCTGTTCGTC CTCAGGAGTG GATCAACATG
    ACTGGTTTCT TGTGCGCTCT GGGAGGTGTT TGTCTCCAAC AGCGCAGCAC GCCGAGTCTG
    GGCACCTACA GTCCTCCAAT GGGCCCCCTG ACCGAACGCA AGGGGTCCAT GATTTCCATG
    AGCTCCTGCG AGGTAAACAG CGAAACATCC CTGAATCGCT TTCTGGAGCG TCTGCTGGCT
    CTGATGGTCT GCACACATGA GAAAGTGGGA GTGCAAATTC GCACCAACGT CAAAGACCTG
    GTGGGACTGG AGCTCAGTCC GGCTCTCTAT TCAATGCTCT TCACTAAGCT GCGCAACAGC
    ATCGGACGCT TCTTTGACAC CCAGGGGCCG GTTCCTATTA CAGATGCAAA CACTCTGTTT
    GTAGAGCAGA CCATTGCTAT CATGAAGAAC TTGCTGGACA GTCACACTGA AGGAAGCTCA
    GAGCACCTTG GTCAAGCCAG CATTGAGACC ATGATGCTCA ATCTAGTTAG GTATGTGCGT
    ATTTTAGGGA ACATTGTTCA TGCCATTCAG ATCAAAACCA AGTTGTGTCA GTTAGTGGAG
    GTGATGATGG AGCGCAGGGA TGACCTCTCC TTCTGCCAGG AGATGAAATT CAGGAATAAA
    ATGGTGGAGT ACCTCACAGA CTGGGTGATG GGAACCTCCA ATCAAGCTGC GGACGATGAT
    GTCAAATGCC TGACCCGCGA TCTGGACCAG GCCAGTATGG AGGCTGTCGT GTCTCTGTTG
    GCTGGTCTTC CTCTACAGCC TGAGGAGGGT GACGGTGTTG AGCTCATGGA GGCCAAATCA
    CAGCTCTTCC TGAAGTACTT CACACTGTTC ATGAACCTGT TGAATGACTG CAGCGAGGTT
    GAGGAAGATG GCCAGCAGGT CGCAGGGAGG AAACGTGGCA TGTCTAGACG ACTGGCATCT
    CTGCGGCACT GCACAGTATT GGCTATGTCT AACTTGCTCA ATGCTAATGT GGATAGCGGC
    CTCATGCACT CAATCGGTCT GGGATACCAT AAGGACCTGC AGACACGGGC TACATTTATG
    GAAGTGCTCA CTAAGATCCT TCAGCAAGGG ACGGAGTTCG ACACGCTTGC AGAGACTGTG
    CTTGCAGATC GCTTTGAGAG ACTGGTCGAG CTGGTCACCA TGATGGGAGA TCAGGGAGAG
    CTGCCCATAG CCATGGCTCT GGCCAATGTG GTCCCATGCT CTCAATGGGA TGAGCTGGCC
    AGAGTGTTAG TGACTCTCTT CGACTCTCGC CATCTGCTGT ATCAGCTGTT GTGGAATATG
    TTCTCCAAAG AGGTGGAGCT GGCAGACTCA ATGCAGACGC TCTTTAGAGG AAATAGCCTA
    GCCAGCAAGA TTATGACCTT CTGCTTCAAG GTGTACGGAG CTGCGTATCT ACAGAAGCTT
    CTGGAGCCGC TGTTGAGAGG AATCATCACT ACACCAGAGT GGCAGCAGAT CAGTTTTGAA
    GTGGACCCTA CTAGACTTGA ACAGGCAGAG AACCTGGAGG AGAACCAGAA GAACCTGCTT
    CAGATCACTG ACCGCTTCTT CCTGGCCATC ATCAACTCCT CGAGCGAGTT TCCTCCACAG
    CTGCGCAGTG TGTGCCACTG TCTGTATCAG GCTACTTGCC ACTCCCTTCT GAATAAAGCC
    ACTGTTAAAG AAAAAAAGGA AAACAAAAAA TCCGTGGTCA GTCAGCGGTT TCCTCAGAAC
    AGCATTGGTG CTGTAGGAAG TGCCATGTTC CTGCGCTTTA TTAACCCAGC TATCGTGTCC
    CCATATGAGG CTGGAATCCT AGACAAGAAA CCCCCACCTC GCATCGAGCG TGGCCTTAAA
    CTCATGTCTA AGATTCTGCA GAGCATTGCT AATCATGTTC TCTTCACAAA AGAGGAGCAT
    ATGAGGCCCT TTAATGATTT TGTCAAAAGC AACTTCGATG CAGCCAGAAG GTTCTTCTTG
    GATATCGCAT CTGATTCTCC ACCAAGTGAT TCTGTGAACC ATAGTCTGTC TTTCATCAGT
    GACGGTAATG TGCTCGCTCT GCACCGGCTA TTATGGAACA ACCAGGAAAG AATTGGCCAA
    TACCTGTCCA GCAACAGGGA CCACAAGGCA GTGGGCCGTA GACCATTTGA CAAGATGGCC
    ACTCTTCTGG CGTACCTGGG TCCTCCTGAA CATAAGCCAG TAGCAGACAC ACACTGGTCC
    AGCCTCAACC TCACCAGCTC AAAGTTTGAG GAGTTCATGA CAAGGCATCA AGTCCATGAG
    AAGGAGGAAT TTAAAGCATT AAAAACCCTC AACATATTTT ACCAAGCTGG CACATCTAAG
    AACGGCAACC CTGTGTTTTA TTACATCGCA CGCAGGTTCA AAACTGGACA AATCAATGGT
    GATCTTCTGA TCTACCACGT CCTGCTGACG CTCAAGCCCT ACTATGCCAA GCATTATGAG
    ATCGTAGTTG ACCTTACTCA TGTGGGCCCC AGTAACCGTT TCAAGACGGA CTTCTTATCC
    AAATGGTTTG TGGTCTTTCC AAACTTCGCC TATGAGAACG TGGCTGCTGT GTACATCTAC
    AACTGCAACA CCTGGGTACG CGAGTACACC AAATACCACG AGCGACTGCT GACTGGCCTC
    AAGGGGAGCA AGAAGCTGGT GTTTATCGAT TCGCCTGCAC GTCTAGCTGA ACACATCGAA
    CCCGAACAGC AGAAGCTGCC CGCAGCTACT TTAGCACTGG AAGAAGACCT CAAAGTGTTC
    CACAACGCCC TAAAGCTTGC ACACAGAGAC ACAAAAGTGT CAATTAAGGT GGGATCCACA
    GCTGTACAGG TGACCTCGGC CGAAAGAACG CGTGTCCTAG GACAGCCTGT GTTCCTAAAT
    GACATCTACT ACGCTTCAGA GATCGAGGAG ATATGCCTGG TGGACGAGAA CCAGTTCACG
    CTAACCATCG CTAACCAGGG CACCCCACTC ACTTTCATGC ACCAGGAGTG TGAAGCCATC
    GTTCAGTCCA TCATCCACAT CCGCACGCGC TGGGAACTTT CCCAACCAGA CTCCATTCCC
    CAACACACCA AAATCAGGCC TAAGGATGTT CCTGGCACCC TGCTCAACAT CGCTCTTCTC
    AACCTGGGAA GCTCCGACCC CAGTCTGAGG TCTGCGGCCT ACAATCTCCT TTGTGCCTTA
    ACCTGCACCT TCAACCTGAA GATTGAAGGC CAGCTTCTGG AAACGTCTGG CTTGTGCATC
    CCAGCCAACA ACACGCTTTT TATTGTGTCC ATTAGCAAAA CGCTAGCAGC TAATGAACCC
    CACCTCACTC TGGAGTTCCT GGAAGAGTGC ATCTCAGGAT TCAGTAAATC AAGTATAGAG
    CTGAAGCATC TGTGTCTAGA ATATATGACT CCTTGGCTCC GTAATTTGGT GCGCTTTTGC
    AAACACAATG ATGACGCCAA ACGGCAGCGT GTCACGGCCA TCCTGGACAA GCTTATTACT
    ATGACCATCA ACGAGAAGCA GATGTACCCC TCCATACAGG CCAAGATATG GGGCAGCTTG
    GGTCAGATCA CAGACCTGCT TGATGTGGTG TTGGACAGCT TTATAAAGAC CAGCGCTACT
    GGCGGTTTAG GATCCATCAA AGCTGAAGTG ATGGCTGACA CTGCTGTAGC TTTGGCGTCA
    GGAAATGTCA AACTGGTCTC CAGCAAGGTG ATTGGCCGAA TGTGTAAGAT CATCGACAAG
    ACGTGTCTGT CTCCCACTCC GACCCTTGAG CAGCACCTCA TGTGGGACGA CATCGCCATC
    CTGGCCCGGT ACATGCTCAT GCTGTCCTTC AACAACTCTC TGGACGTGGC CGCTCATCTG
    CCATACCTGT TCCACGTCGT CACCCTGCTG GTGGCCACGG GCCCTCTCTC GCTGCGTGCA
    TCAACACACG GCCTGGTCAT CAACATCATT CACTCGCTCT GCACATGCTC ACAACTCAAC
    TTCAGTGAGG AGACTAAACA GGTGCTGCGC CTGAGTCTGA CTGAGTTCTC CCTGCCCAAG
    TTCTACCTGC TGTTCGGCAT CAGTAAGGTC AAATCTGCGG CGGTCATCGC TTTCCGTTCC
    AGCTACAGAG ACCGCTCATT CTCTCCAGGC TCTTATGAGA GAGAGACATT TGCTCTGTCT
    TCCCTGGAAA CAGTCACGGA GGCTCTTTTG GAGATCATGG AGGCCTGCAT GAGGGACATT
    CCTGGATGCA AATGGCTAGA TCAGTGGACT GAATTGGCGC AAAAGTTTGC GTTCCAGTAT
    AACCCCTCTC TTCAGCCTCG GGCGCTGGTG GTGTTCGGCT GCATCAGTAA ACGAGTGACT
    CACGGACAGC TAAAGCAGAT CATTCGTATT CTCAGTAAAG CCAGTCCTCT ACTCAGCATT
    GATCGGGGGC TGGAGAGCTG CTTAAAGGGC CCAGATAATT ACAACAGCCA GGTTCTCATC
    GAAGCCACAG TCATCGCTCT CACCAAACTA CAGCCTCTCC TCGATCAGGA TTCTCCTATG
    CATAAAGCTC TGTTCTGGGT GGCCGTGGCC GTGCTTCAGT TGGATGAAGT GAATCTGTAC
    TCCGCCGGCA CTGCCTTACT GGAGCAGAAC CTGCACACAC TGGACAGCCT CCGAGTGTTT
    AATGATAAGA GTCCTGAAGA GGTTTTTATG GAGATCCGGA AACCTCTAGA GTGGCACTGT
    AAACAAATGG ACCATTTTGT GGGACTCAAC TTTGCCTCCA ACTTCAATTT TGCATTAGTG
    GGCCATTTAC TTAAAGGATA CAGACATCCC TCTTCAACAA CAGTTGCCAG GACAGTTCGT
    ATCCTGCATA CACTCCTGGG CCTGGTGGGA AAACACCGCA GCTGTGACAA GTTTGAGGTG
    AACACGCAGA GTGTGGCCTA TCTGGCAGCT TTGCTCACAG TGTCAGAGGA AGTTAGGAGC
    CGCTGCAGTT TAAAGCACAG GAAATCATTG CTGCTTTCAG AAGTTACAAT GGAGAACTTC
    CCCATGGACA CATACACCGT TTACGACACT GAACCCGGGT GCAGGCTACT GCGTGAAAAC
    CAGCCTTGGG CTTCTCCGAA AGTGTCAGAG CGCAGTCTTG CACCTCCGTA CTCCACAATA
    GGGCAGACCA GCCTTCGTCC ACGCAAATCC ATGAGTGTAG ACATGGGCCA GCCGTCTCAG
    GCCAATGCGA AGAAACTACT GGGCACTAGA AAGAGTTTCG ATCACTTAAT ATCAGAATCC
    AAAGCCCCAA AAAGACCAGA CATGGAGTCG GGCATCACCA CACCTCCAAA GATGAGGCGG
    GTTTCTGAAA ATGACTATGA GATCGAAACT CAGCGAATAG CCAACTCTCA TTTACGCAAA
    GTGTCTGTGT CTGAATCCAA TGTCCTCCTG GACGAGAACG TACTTACGGA CCCCAAGATC
    CAGACGTTGC TCCTCACTGT ACTGGCAACA CTGGTGAAGT ACACTACAGA CGAGTTTCAT
    CAGCGGATCT TGTATGAGTA TTTGGCTGAG GCCAGTGTGG TCTTCCCCAA GGTCTTTCCA
    GTTGTGCACA ACCTGCTGGA CTCCAAGTTA AGCACCGTCC TTTCCATGTG CCAGGACCCG
    AACCTTCTGA ACCCCGTCCA TGGCATCATT CAGAACGTGG TCTATCATGA GGAGTCTCCC
    CCTCAGTACC AGCCGTCCTG CTTGCAGAGC TTTGGTTTCA ATGGTCTTTG GAGGTTTGCC
    GGGCCGTTCT CTAAGCAAAC CCAAATTCCA GACTACGCCG AGCTGGTGGT TAAATTTTTG
    GATGCTTTGA TTGACACGTA TCTCCCGGGC ATTGAGGAGG AAGGAGGAGA AGAGTCACTG
    CGGACCCCGA CCTCGCCATA TCCACCAACC GTCCAGAGCC AGCTGAGCAT CACAGCCAAT
    CTCAACCTGT CCAACTCCAT GACATCACTT GCCACATCAC AGCACTCCCC AGGCATCGAC
    AAGGAGAACG TTGAGATTTC TCCCACCTCG AGTCTGTCCA CCAGTGGGCG AATGCGGCAC
    GGCTCAGCAA GCCAGGTGCA GAAGCAGCGC AGCGCAGGCA GCTTCAAACG GCCAAGCATC
    AAAAAGATCG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa39878
    Clone ID Related Accession (Same CDS sequence) XM_009291769.2 , XM_009291769.3
    Accession Version XM_009291769.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8292bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product neurofibromin isoform X8
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009291769.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGGCGGCGC ACAAGCCCGT GGAATGGGTC CAGGCCATCA TTAACAGATT CGACGAACAG 
    CTCCCAATCA AAACAGGACA GCAGAGCACC CACACCAAAG TGAGTACAGA GCACAACAAA
    GAATGTTTGA TCAACATCTC CAAGTACAAA TTTGCCCTCG TCATCAATGG CCTGACCAAC
    ATACTCAAAA ATGTGAACAA CATGCGGATA TTTGGCGAAG CTGCTGAGAA GAACCTCTAC
    TTCTCCCAGC TGATCATACT GGACACACTG GAGAAGTGTC TAGCAGGGCA ACCTAAGGAT
    TGCCTGAGGC TGGATGAGGC TATGTTGGTA AAACAGTTGC TGCCTGAAAT CTGTCACTTC
    CTGCACTCGT ACCGTGAGGG CCAGCAACAC GCCACTCAGC TGCGCAGCTC CGCCTCTGCT
    GTGCTTTTCT CTCTCAGCTG CAACAACTTT AATGCCGTCT TCAGTCGTAT CTCCACCAGG
    TTGCAAGAGC TCACAGTTTG TACAGAGGAC ACAGTGGATG TCCATGACAT TGAGCTCATT
    CAGTATATCA ATGTAGATTG CTCCAAGTTA AAGAGACTCC TACAAGAAGC AGTACTTAAG
    TTCAAGTCTT TGAAAAAGCC GGCTCAGCTT GCAGTAATCA GCAGTTTGGA AAAGGCTTTC
    TGGAACTGGG TTGAACACTA CCCTGATGAT TTCACTATGC TCTATCAGAG ACCCCAGGCC
    GACATGGCCG ACTGTGCAGA GAGACTGTTC GACCTAGTGG ACAGTTTTGC GGAAAGCGCA
    AAGAGAAAAG CTGCTGTGTG GCCACTGCAG ATCATCCTCC TCATCCTCTG TCCAGAGATC
    ACGCAGAGCA TCACCAAAGA AGTAGGGGGA GAGGAGAAAG CTAACAAGAA GTCATTTGTA
    GACAGTTTAC GAAAGGCTCT TGGCCAACAC AGCGGCAGCA AACAATTGAC GGAAAGTGCA
    GCTGTTGCAT GCGTCAAACT CTGCAAAGCC TGCACATATA TCAACTGGGA GGATAATTCT
    GTCATCTTTC TCCTGGTTCA GTCCATCGTC ATGGACCTTA AGGGTCTCCT GTTTAACCCC
    ACCAAACCTT TCTCAAGAGG TACGGGCTGC CAGAGCGCCG ATGTAGATTT GATGATCGAC
    TGCTTTGTGT CATGTTATCG CATCAATCCT CGCAACAACC AGCATTTTAA GGTGTGTCTG
    GCCTCGACTT CTCCTCCTAC CTTCCATTTC GTCCTTGTCA ACTCCCTGCA CAAAATTATC
    ACAAATTCTC ACCTGGACTG GTGGCCGAAG ATTGACGCCA TCTACTGCTA CTCAGGAGAA
    CTACGCACCA TGTTTGCAGA CACACTGGGT CGAGTGATTC AGAGTCTCAG TGCACATCCG
    CCTCTGAGGA TGACATCAAG TTTGACATTC AAAGAGAAAA TGACAACAAG TTTGAAGTTC
    AAGGAAAAGT CCACAGATCT TGATTCGCGT TCTTTGCTGC TGGCACTGGT CAAACTTATC
    CACGCTGACC CCAAACTAAT GCTGCACAAT CCGGGAAAAC CTGGTCAGGA GATTCAGAAC
    AGCACAGCTG AGATCATCAA AGGTCTGGTT CAGCTGGTTC CTCTTACCCA CAGTGCTGAG
    CTTTCCCAAG AGGCCATGGA GGCATTGCTT GTTTTACATC AGCCAGAAAC CATTGAGCTA
    TGGAACCCGG ACGCTCCCAT TGAGACCTTC TGGGACATCA GCTCTCAGGT GTTATTTCTA
    ATTTGTAAGA AGCTGATTGG ACACCAGATG ATCAACAGTA CAGAGATACT CAAGTGGCTC
    AGGGAGATTC TCATATGCAG GAACAAGTTT CTATTAAAGA ACAAGGAGTG TGCTACACAG
    GGCAGTCTGA TCCCCATCAG CAGACAGGCT CAGACCAAGC TGGAGGTGTG TCTGTACACG
    TTCCTGTGGA GTCCTGATGC AGAGGCTGTT TTGGTGGCCA TGTCCTGTTT CAGGCATCTC
    TGTGAGGAGG CCGATATTCG CTGCAGTGTC GATGAGCTGC CTGTTCACAC AGTCCTACCC
    AATTACAACA CCTTCATTGA GCTTGCATCC GTCAGCAATA TGATGGCCAC AGGTCGCAAC
    ACACTACAAA AAAGAGTGAT GGCACTTTTA AGAAGAATAA AGCACCCTAC AGCTGGAAAT
    GTAGAGGCAT GGGAAGACAC ATATGCAAAG TGGGAGCAAG CTACAAAACA GATACTCAAC
    CACCCAAAAA ACAAAGTGGA AGATGGACAG GTAGTTAACA TAAGATTGGT CAATTATTTC
    ATCTTTTTTT CCATTCATGG TCATCTATTT TCTGTTCGTC CTCAGGAGTG GATCAACATG
    ACTGGTTTCT TGTGCGCTCT GGGAGGTGTT TGTCTCCAAC AGCGCAGCAC GCCGAGTCTG
    GGCACCTACA GTCCTCCAAT GGGCCCCCTG ACCGAACGCA AGGGGTCCAT GATTTCCATG
    AGCTCCTGCG AGGTAAACAG CGAAACATCC CTGAATCGCT TTCTGGAGCG TCTGCTGGCT
    CTGATGGTCT GCACACATGA GAAAGTGGGA GTGCAAATTC GCACCAACGT CAAAGACCTG
    GTGGGACTGG AGCTCAGTCC GGCTCTCTAT TCAATGCTCT TCACTAAGCT GCGCAACAGC
    ATCGGACGCT TCTTTGACAC CCAGGGGCCG GTTCCTATTA CAGATGCAAA CACTCTGTTT
    GTAGAGCAGA CCATTGCTAT CATGAAGAAC TTGCTGGACA GTCACACTGA AGGAAGCTCA
    GAGCACCTTG GTCAAGCCAG CATTGAGACC ATGATGCTCA ATCTAGTTAG GTATGTGCGT
    ATTTTAGGGA ACATTGTTCA TGCCATTCAG ATCAAAACCA AGTTGTGTCA GTTAGTGGAG
    GTGATGATGG AGCGCAGGGA TGACCTCTCC TTCTGCCAGG AGATGAAATT CAGGAATAAA
    ATGGTGGAGT ACCTCACAGA CTGGGTGATG GGAACCTCCA ATCAAGCTGC GGACGATGAT
    GTCAAATGCC TGACCCGCGA TCTGGACCAG GCCAGTATGG AGGCTGTCGT GTCTCTGTTG
    GCTGGTCTTC CTCTACAGCC TGAGGAGGGT GACGGTGTTG AGCTCATGGA GGCCAAATCA
    CAGCTCTTCC TGAAGTACTT CACACTGTTC ATGAACCTGT TGAATGACTG CAGCGAGGTT
    GAGGAAGATG GCCAGCAGGT CGCAGGGAGG AAACGTGGCA TGTCTAGACG ACTGGCATCT
    CTGCGGCACT GCACAGTATT GGCTATGTCT AACTTGCTCA ATGCTAATGT GGATAGCGGC
    CTCATGCACT CAATCGGTCT GGGATACCAT AAGGACCTGC AGACACGGGC TACATTTATG
    GAAGTGCTCA CTAAGATCCT TCAGCAAGGG ACGGAGTTCG ACACGCTTGC AGAGACTGTG
    CTTGCAGATC GCTTTGAGAG ACTGGTCGAG CTGGTCACCA TGATGGGAGA TCAGGGAGAG
    CTGCCCATAG CCATGGCTCT GGCCAATGTG GTCCCATGCT CTCAATGGGA TGAGCTGGCC
    AGAGTGTTAG TGACTCTCTT CGACTCTCGC CATCTGCTGT ATCAGCTGTT GTGGAATATG
    TTCTCCAAAG AGGTGGAGCT GGCAGACTCA ATGCAGACGC TCTTTAGAGG AAATAGCCTA
    GCCAGCAAGA TTATGACCTT CTGCTTCAAG GTGTACGGAG CTGCGTATCT ACAGAAGCTT
    CTGGAGCCGC TGTTGAGAGG AATCATCACT ACACCAGAGT GGCAGCAGAT CAGTTTTGAA
    GTGGACCCTA CTAGACTTGA ACAGGCAGAG AACCTGGAGG AGAACCAGAA GAACCTGCTT
    CAGATCACTG ACCGCTTCTT CCTGGCCATC ATCAACTCCT CGAGCGAGTT TCCTCCACAG
    CTGCGCAGTG TGTGCCACTG TCTGTATCAG GTGGTCAGTC AGCGGTTTCC TCAGAACAGC
    ATTGGTGCTG TAGGAAGTGC CATGTTCCTG CGCTTTATTA ACCCAGCTAT CGTGTCCCCA
    TATGAGGCTG GAATCCTAGA CAAGAAACCC CCACCTCGCA TCGAGCGTGG CCTTAAACTC
    ATGTCTAAGA TTCTGCAGAG CATTGCTAAT CATGTTCTCT TCACAAAAGA GGAGCATATG
    AGGCCCTTTA ATGATTTTGT CAAAAGCAAC TTCGATGCAG CCAGAAGGTT CTTCTTGGAT
    ATCGCATCTG ATTCTCCACC AAGTGATTCT GTGAACCATA GTCTGTCTTT CATCAGTGAC
    GGTAATGTGC TCGCTCTGCA CCGGCTATTA TGGAACAACC AGGAAAGAAT TGGCCAATAC
    CTGTCCAGCA ACAGGGACCA CAAGGCAGTG GGCCGTAGAC CATTTGACAA GATGGCCACT
    CTTCTGGCGT ACCTGGGTCC TCCTGAACAT AAGCCAGTAG CAGACACACA CTGGTCCAGC
    CTCAACCTCA CCAGCTCAAA GTTTGAGGAG TTCATGACAA GGCATCAAGT CCATGAGAAG
    GAGGAATTTA AAGCATTAAA AACCCTCAAC ATATTTTACC AAGCTGGCAC ATCTAAGAAC
    GGCAACCCTG TGTTTTATTA CATCGCACGC AGGTTCAAAA CTGGACAAAT CAATGGTGAT
    CTTCTGATCT ACCACGTCCT GCTGACGCTC AAGCCCTACT ATGCCAAGCA TTATGAGATC
    GTAGTTGACC TTACTCATGT GGGCCCCAGT AACCGTTTCA AGACGGACTT CTTATCCAAA
    TGGTTTGTGG TCTTTCCAAA CTTCGCCTAT GAGAACGTGG CTGCTGTGTA CATCTACAAC
    TGCAACACCT GGGTACGCGA GTACACCAAA TACCACGAGC GACTGCTGAC TGGCCTCAAG
    GGGAGCAAGA AGCTGGTGTT TATCGATTCG CCTGCACGTC TAGCTGAACA CATCGAACCC
    GAACAGCAGA AGCTGCCCGC AGCTACTTTA GCACTGGAAG AAGACCTCAA AGTGTTCCAC
    AACGCCCTAA AGCTTGCACA CAGAGACACA AAAGTGTCAA TTAAGGTGGG ATCCACAGCT
    GTACAGGTGA CCTCGGCCGA AAGAACGCGT GTCCTAGGAC AGCCTGTGTT CCTAAATGAC
    ATCTACTACG CTTCAGAGAT CGAGGAGATA TGCCTGGTGG ACGAGAACCA GTTCACGCTA
    ACCATCGCTA ACCAGGGCAC CCCACTCACT TTCATGCACC AGGAGTGTGA AGCCATCGTT
    CAGTCCATCA TCCACATCCG CACGCGCTGG GAACTTTCCC AACCAGACTC CATTCCCCAA
    CACACCAAAA TCAGGCCTAA GGATGTTCCT GGCACCCTGC TCAACATCGC TCTTCTCAAC
    CTGGGAAGCT CCGACCCCAG TCTGAGGTCT GCGGCCTACA ATCTCCTTTG TGCCTTAACC
    TGCACCTTCA ACCTGAAGAT TGAAGGCCAG CTTCTGGAAA CGTCTGGCTT GTGCATCCCA
    GCCAACAACA CGCTTTTTAT TGTGTCCATT AGCAAAACGC TAGCAGCTAA TGAACCCCAC
    CTCACTCTGG AGTTCCTGGA AGAGTGCATC TCAGGATTCA GTAAATCAAG TATAGAGCTG
    AAGCATCTGT GTCTAGAATA TATGACTCCT TGGCTCCGTA ATTTGGTGCG CTTTTGCAAA
    CACAATGATG ACGCCAAACG GCAGCGTGTC ACGGCCATCC TGGACAAGCT TATTACTATG
    ACCATCAACG AGAAGCAGAT GTACCCCTCC ATACAGGCCA AGATATGGGG CAGCTTGGGT
    CAGATCACAG ACCTGCTTGA TGTGGTGTTG GACAGCTTTA TAAAGACCAG CGCTACTGGC
    GGTTTAGGAT CCATCAAAGC TGAAGTGATG GCTGACACTG CTGTAGCTTT GGCGTCAGGA
    AATGTCAAAC TGGTCTCCAG CAAGGTGATT GGCCGAATGT GTAAGATCAT CGACAAGACG
    TGTCTGTCTC CCACTCCGAC CCTTGAGCAG CACCTCATGT GGGACGACAT CGCCATCCTG
    GCCCGGTACA TGCTCATGCT GTCCTTCAAC AACTCTCTGG ACGTGGCCGC TCATCTGCCA
    TACCTGTTCC ACGTCGTCAC CCTGCTGGTG GCCACGGGCC CTCTCTCGCT GCGTGCATCA
    ACACACGGCC TGGTCATCAA CATCATTCAC TCGCTCTGCA CATGCTCACA ACTCAACTTC
    AGTGAGGAGA CTAAACAGGT GCTGCGCCTG AGTCTGACTG AGTTCTCCCT GCCCAAGTTC
    TACCTGCTGT TCGGCATCAG TAAGGTCAAA TCTGCGGCGG TCATCGCTTT CCGTTCCAGC
    TACAGAGACC GCTCATTCTC TCCAGGCTCT TATGAGAGAG AGACATTTGC TCTGTCTTCC
    CTGGAAACAG TCACGGAGGC TCTTTTGGAG ATCATGGAGG CCTGCATGAG GGACATTCCT
    GGATGCAAAT GGCTAGATCA GTGGACTGAA TTGGCGCAAA AGTTTGCGTT CCAGTATAAC
    CCCTCTCTTC AGCCTCGGGC GCTGGTGGTG TTCGGCTGCA TCAGTAAACG AGTGACTCAC
    GGACAGCTAA AGCAGATCAT TCGTATTCTC AGTAAAGCCA GTCCTCTACT CAGCATTGAT
    CGGGGGCTGG AGAGCTGCTT AAAGGGCCCA GATAATTACA ACAGCCAGGT TCTCATCGAA
    GCCACAGTCA TCGCTCTCAC CAAACTACAG CCTCTCCTCG ATCAGGATTC TCCTATGCAT
    AAAGCTCTGT TCTGGGTGGC CGTGGCCGTG CTTCAGTTGG ATGAAGTGAA TCTGTACTCC
    GCCGGCACTG CCTTACTGGA GCAGAACCTG CACACACTGG ACAGCCTCCG AGTGTTTAAT
    GATAAGAGTC CTGAAGAGGT TTTTATGGAG ATCCGGAAAC CTCTAGAGTG GCACTGTAAA
    CAAATGGACC ATTTTGTGGG ACTCAACTTT GCCTCCAACT TCAATTTTGC ATTAGTGGGC
    CATTTACTTA AAGGATACAG ACATCCCTCT TCAACAACAG TTGCCAGGAC AGTTCGTATC
    CTGCATACAC TCCTGGGCCT GGTGGGAAAA CACCGCAGCT GTGACAAGTT TGAGGTGAAC
    ACGCAGAGTG TGGCCTATCT GGCAGCTTTG CTCACAGTGT CAGAGGAAGT TAGGAGCCGC
    TGCAGTTTAA AGCACAGGAA ATCATTGCTG CTTTCAGAAG TTACAATGGA GAACTTCCCC
    ATGGACACAT ACACCGTTTA CGACACTGAA CCCGGGTGCA GGCTACTGCG TGAAAACCAG
    CCTTGGGCTT CTCCGAAAGT GTCAGAGCGC AGTCTTGCAC CTCCGTACTC CACAATAGGG
    CAGACCAGCC TTCGTCCACG CAAATCCATG AGTGTAGACA TGGGCCAGCC GTCTCAGGCC
    AATGCGAAGA AACTACTGGG CACTAGAAAG AGTTTCGATC ACTTAATATC AGAATCCAAA
    GCCCCAAAAA GACCAGACAT GGAGTCGGGC ATCACCACAC CTCCAAAGAT GAGGCGGGTT
    TCTGAAAATG ACTATGAGAT CGAAACTCAG CGAATAGCCA ACTCTCATTT ACGCAAAGTG
    TCTGTGTCTG AATCCAATGT CCTCCTGGAC GAGAACGTAC TTACGGACCC CAAGATCCAG
    ACGTTGCTCC TCACTGTACT GGCAACACTG GTGAAGTACA CTACAGACGA GTTTCATCAG
    CGGATCTTGT ATGAGTATTT GGCTGAGGCC AGTGTGGTCT TCCCCAAGGT CTTTCCAGTT
    GTGCACAACC TGCTGGACTC CAAGTTAAGC ACCGTCCTTT CCATGTGCCA GGACCCGAAC
    CTTCTGAACC CCGTCCATGG CATCATTCAG AACGTGGTCT ATCATGAGGA GTCTCCCCCT
    CAGTACCAGC CGTCCTGCTT GCAGAGCTTT GGTTTCAATG GTCTTTGGAG GTTTGCCGGG
    CCGTTCTCTA AGCAAACCCA AATTCCAGAC TACGCCGAGC TGGTGGTTAA ATTTTTGGAT
    GCTTTGATTG ACACGTATCT CCCGGGCATT GAGGAGGAAG GAGGAGAAGA GTCACTGCGG
    ACCCCGACCT CGCCATATCC ACCAACCGTC CAGAGCCAGC TGAGCATCAC AGCCAATCTC
    AACCTGTCCA ACTCCATGAC ATCACTTGCC ACATCACAGC ACTCCCCAGG CATCGACAAG
    GAGAACGTTG AGATTTCTCC CACCTCGAGT CTGTCCACCA GTGGGCGAAT GCGGCACGGC
    TCAGCAAGCC AGGTGCAGAA GCAGCGCAGC GCAGGCAGCT TCAAACGGCC AAGCATCAAA
    AAGATCGTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290044.1
    CDS252..8543
    Translation

    Target ORF information:

    RefSeq Version XM_009291769.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio neurofibromin 1a (nf1a), transcript variant X8, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009291769.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGGCGGCGC ACAAGCCCGT GGAATGGGTC CAGGCCATCA TTAACAGATT CGACGAACAG 
    CTCCCAATCA AAACAGGACA GCAGAGCACC CACACCAAAG TGAGTACAGA GCACAACAAA
    GAATGTTTGA TCAACATCTC CAAGTACAAA TTTGCCCTCG TCATCAATGG CCTGACCAAC
    ATACTCAAAA ATGTGAACAA CATGCGGATA TTTGGCGAAG CTGCTGAGAA GAACCTCTAC
    TTCTCCCAGC TGATCATACT GGACACACTG GAGAAGTGTC TAGCAGGGCA ACCTAAGGAT
    TGCCTGAGGC TGGATGAGGC TATGTTGGTA AAACAGTTGC TGCCTGAAAT CTGTCACTTC
    CTGCACTCGT ACCGTGAGGG CCAGCAACAC GCCACTCAGC TGCGCAGCTC CGCCTCTGCT
    GTGCTTTTCT CTCTCAGCTG CAACAACTTT AATGCCGTCT TCAGTCGTAT CTCCACCAGG
    TTGCAAGAGC TCACAGTTTG TACAGAGGAC ACAGTGGATG TCCATGACAT TGAGCTCATT
    CAGTATATCA ATGTAGATTG CTCCAAGTTA AAGAGACTCC TACAAGAAGC AGTACTTAAG
    TTCAAGTCTT TGAAAAAGCC GGCTCAGCTT GCAGTAATCA GCAGTTTGGA AAAGGCTTTC
    TGGAACTGGG TTGAACACTA CCCTGATGAT TTCACTATGC TCTATCAGAG ACCCCAGGCC
    GACATGGCCG ACTGTGCAGA GAGACTGTTC GACCTAGTGG ACAGTTTTGC GGAAAGCGCA
    AAGAGAAAAG CTGCTGTGTG GCCACTGCAG ATCATCCTCC TCATCCTCTG TCCAGAGATC
    ACGCAGAGCA TCACCAAAGA AGTAGGGGGA GAGGAGAAAG CTAACAAGAA GTCATTTGTA
    GACAGTTTAC GAAAGGCTCT TGGCCAACAC AGCGGCAGCA AACAATTGAC GGAAAGTGCA
    GCTGTTGCAT GCGTCAAACT CTGCAAAGCC TGCACATATA TCAACTGGGA GGATAATTCT
    GTCATCTTTC TCCTGGTTCA GTCCATCGTC ATGGACCTTA AGGGTCTCCT GTTTAACCCC
    ACCAAACCTT TCTCAAGAGG TACGGGCTGC CAGAGCGCCG ATGTAGATTT GATGATCGAC
    TGCTTTGTGT CATGTTATCG CATCAATCCT CGCAACAACC AGCATTTTAA GGTGTGTCTG
    GCCTCGACTT CTCCTCCTAC CTTCCATTTC GTCCTTGTCA ACTCCCTGCA CAAAATTATC
    ACAAATTCTC ACCTGGACTG GTGGCCGAAG ATTGACGCCA TCTACTGCTA CTCAGGAGAA
    CTACGCACCA TGTTTGCAGA CACACTGGGT CGAGTGATTC AGAGTCTCAG TGCACATCCG
    CCTCTGAGGA TGACATCAAG TTTGACATTC AAAGAGAAAA TGACAACAAG TTTGAAGTTC
    AAGGAAAAGT CCACAGATCT TGATTCGCGT TCTTTGCTGC TGGCACTGGT CAAACTTATC
    CACGCTGACC CCAAACTAAT GCTGCACAAT CCGGGAAAAC CTGGTCAGGA GATTCAGAAC
    AGCACAGCTG AGATCATCAA AGGTCTGGTT CAGCTGGTTC CTCTTACCCA CAGTGCTGAG
    CTTTCCCAAG AGGCCATGGA GGCATTGCTT GTTTTACATC AGCCAGAAAC CATTGAGCTA
    TGGAACCCGG ACGCTCCCAT TGAGACCTTC TGGGACATCA GCTCTCAGGT GTTATTTCTA
    ATTTGTAAGA AGCTGATTGG ACACCAGATG ATCAACAGTA CAGAGATACT CAAGTGGCTC
    AGGGAGATTC TCATATGCAG GAACAAGTTT CTATTAAAGA ACAAGGAGTG TGCTACACAG
    GGCAGTCTGA TCCCCATCAG CAGACAGGCT CAGACCAAGC TGGAGGTGTG TCTGTACACG
    TTCCTGTGGA GTCCTGATGC AGAGGCTGTT TTGGTGGCCA TGTCCTGTTT CAGGCATCTC
    TGTGAGGAGG CCGATATTCG CTGCAGTGTC GATGAGCTGC CTGTTCACAC AGTCCTACCC
    AATTACAACA CCTTCATTGA GCTTGCATCC GTCAGCAATA TGATGGCCAC AGGTCGCAAC
    ACACTACAAA AAAGAGTGAT GGCACTTTTA AGAAGAATAA AGCACCCTAC AGCTGGAAAT
    GTAGAGGCAT GGGAAGACAC ATATGCAAAG TGGGAGCAAG CTACAAAACA GATACTCAAC
    CACCCAAAAA ACAAAGTGGA AGATGGACAG GTAGTTAACA TAAGATTGGT CAATTATTTC
    ATCTTTTTTT CCATTCATGG TCATCTATTT TCTGTTCGTC CTCAGGAGTG GATCAACATG
    ACTGGTTTCT TGTGCGCTCT GGGAGGTGTT TGTCTCCAAC AGCGCAGCAC GCCGAGTCTG
    GGCACCTACA GTCCTCCAAT GGGCCCCCTG ACCGAACGCA AGGGGTCCAT GATTTCCATG
    AGCTCCTGCG AGGTAAACAG CGAAACATCC CTGAATCGCT TTCTGGAGCG TCTGCTGGCT
    CTGATGGTCT GCACACATGA GAAAGTGGGA GTGCAAATTC GCACCAACGT CAAAGACCTG
    GTGGGACTGG AGCTCAGTCC GGCTCTCTAT TCAATGCTCT TCACTAAGCT GCGCAACAGC
    ATCGGACGCT TCTTTGACAC CCAGGGGCCG GTTCCTATTA CAGATGCAAA CACTCTGTTT
    GTAGAGCAGA CCATTGCTAT CATGAAGAAC TTGCTGGACA GTCACACTGA AGGAAGCTCA
    GAGCACCTTG GTCAAGCCAG CATTGAGACC ATGATGCTCA ATCTAGTTAG GTATGTGCGT
    ATTTTAGGGA ACATTGTTCA TGCCATTCAG ATCAAAACCA AGTTGTGTCA GTTAGTGGAG
    GTGATGATGG AGCGCAGGGA TGACCTCTCC TTCTGCCAGG AGATGAAATT CAGGAATAAA
    ATGGTGGAGT ACCTCACAGA CTGGGTGATG GGAACCTCCA ATCAAGCTGC GGACGATGAT
    GTCAAATGCC TGACCCGCGA TCTGGACCAG GCCAGTATGG AGGCTGTCGT GTCTCTGTTG
    GCTGGTCTTC CTCTACAGCC TGAGGAGGGT GACGGTGTTG AGCTCATGGA GGCCAAATCA
    CAGCTCTTCC TGAAGTACTT CACACTGTTC ATGAACCTGT TGAATGACTG CAGCGAGGTT
    GAGGAAGATG GCCAGCAGGT CGCAGGGAGG AAACGTGGCA TGTCTAGACG ACTGGCATCT
    CTGCGGCACT GCACAGTATT GGCTATGTCT AACTTGCTCA ATGCTAATGT GGATAGCGGC
    CTCATGCACT CAATCGGTCT GGGATACCAT AAGGACCTGC AGACACGGGC TACATTTATG
    GAAGTGCTCA CTAAGATCCT TCAGCAAGGG ACGGAGTTCG ACACGCTTGC AGAGACTGTG
    CTTGCAGATC GCTTTGAGAG ACTGGTCGAG CTGGTCACCA TGATGGGAGA TCAGGGAGAG
    CTGCCCATAG CCATGGCTCT GGCCAATGTG GTCCCATGCT CTCAATGGGA TGAGCTGGCC
    AGAGTGTTAG TGACTCTCTT CGACTCTCGC CATCTGCTGT ATCAGCTGTT GTGGAATATG
    TTCTCCAAAG AGGTGGAGCT GGCAGACTCA ATGCAGACGC TCTTTAGAGG AAATAGCCTA
    GCCAGCAAGA TTATGACCTT CTGCTTCAAG GTGTACGGAG CTGCGTATCT ACAGAAGCTT
    CTGGAGCCGC TGTTGAGAGG AATCATCACT ACACCAGAGT GGCAGCAGAT CAGTTTTGAA
    GTGGACCCTA CTAGACTTGA ACAGGCAGAG AACCTGGAGG AGAACCAGAA GAACCTGCTT
    CAGATCACTG ACCGCTTCTT CCTGGCCATC ATCAACTCCT CGAGCGAGTT TCCTCCACAG
    CTGCGCAGTG TGTGCCACTG TCTGTATCAG GTGGTCAGTC AGCGGTTTCC TCAGAACAGC
    ATTGGTGCTG TAGGAAGTGC CATGTTCCTG CGCTTTATTA ACCCAGCTAT CGTGTCCCCA
    TATGAGGCTG GAATCCTAGA CAAGAAACCC CCACCTCGCA TCGAGCGTGG CCTTAAACTC
    ATGTCTAAGA TTCTGCAGAG CATTGCTAAT CATGTTCTCT TCACAAAAGA GGAGCATATG
    AGGCCCTTTA ATGATTTTGT CAAAAGCAAC TTCGATGCAG CCAGAAGGTT CTTCTTGGAT
    ATCGCATCTG ATTCTCCACC AAGTGATTCT GTGAACCATA GTCTGTCTTT CATCAGTGAC
    GGTAATGTGC TCGCTCTGCA CCGGCTATTA TGGAACAACC AGGAAAGAAT TGGCCAATAC
    CTGTCCAGCA ACAGGGACCA CAAGGCAGTG GGCCGTAGAC CATTTGACAA GATGGCCACT
    CTTCTGGCGT ACCTGGGTCC TCCTGAACAT AAGCCAGTAG CAGACACACA CTGGTCCAGC
    CTCAACCTCA CCAGCTCAAA GTTTGAGGAG TTCATGACAA GGCATCAAGT CCATGAGAAG
    GAGGAATTTA AAGCATTAAA AACCCTCAAC ATATTTTACC AAGCTGGCAC ATCTAAGAAC
    GGCAACCCTG TGTTTTATTA CATCGCACGC AGGTTCAAAA CTGGACAAAT CAATGGTGAT
    CTTCTGATCT ACCACGTCCT GCTGACGCTC AAGCCCTACT ATGCCAAGCA TTATGAGATC
    GTAGTTGACC TTACTCATGT GGGCCCCAGT AACCGTTTCA AGACGGACTT CTTATCCAAA
    TGGTTTGTGG TCTTTCCAAA CTTCGCCTAT GAGAACGTGG CTGCTGTGTA CATCTACAAC
    TGCAACACCT GGGTACGCGA GTACACCAAA TACCACGAGC GACTGCTGAC TGGCCTCAAG
    GGGAGCAAGA AGCTGGTGTT TATCGATTCG CCTGCACGTC TAGCTGAACA CATCGAACCC
    GAACAGCAGA AGCTGCCCGC AGCTACTTTA GCACTGGAAG AAGACCTCAA AGTGTTCCAC
    AACGCCCTAA AGCTTGCACA CAGAGACACA AAAGTGTCAA TTAAGGTGGG ATCCACAGCT
    GTACAGGTGA CCTCGGCCGA AAGAACGCGT GTCCTAGGAC AGCCTGTGTT CCTAAATGAC
    ATCTACTACG CTTCAGAGAT CGAGGAGATA TGCCTGGTGG ACGAGAACCA GTTCACGCTA
    ACCATCGCTA ACCAGGGCAC CCCACTCACT TTCATGCACC AGGAGTGTGA AGCCATCGTT
    CAGTCCATCA TCCACATCCG CACGCGCTGG GAACTTTCCC AACCAGACTC CATTCCCCAA
    CACACCAAAA TCAGGCCTAA GGATGTTCCT GGCACCCTGC TCAACATCGC TCTTCTCAAC
    CTGGGAAGCT CCGACCCCAG TCTGAGGTCT GCGGCCTACA ATCTCCTTTG TGCCTTAACC
    TGCACCTTCA ACCTGAAGAT TGAAGGCCAG CTTCTGGAAA CGTCTGGCTT GTGCATCCCA
    GCCAACAACA CGCTTTTTAT TGTGTCCATT AGCAAAACGC TAGCAGCTAA TGAACCCCAC
    CTCACTCTGG AGTTCCTGGA AGAGTGCATC TCAGGATTCA GTAAATCAAG TATAGAGCTG
    AAGCATCTGT GTCTAGAATA TATGACTCCT TGGCTCCGTA ATTTGGTGCG CTTTTGCAAA
    CACAATGATG ACGCCAAACG GCAGCGTGTC ACGGCCATCC TGGACAAGCT TATTACTATG
    ACCATCAACG AGAAGCAGAT GTACCCCTCC ATACAGGCCA AGATATGGGG CAGCTTGGGT
    CAGATCACAG ACCTGCTTGA TGTGGTGTTG GACAGCTTTA TAAAGACCAG CGCTACTGGC
    GGTTTAGGAT CCATCAAAGC TGAAGTGATG GCTGACACTG CTGTAGCTTT GGCGTCAGGA
    AATGTCAAAC TGGTCTCCAG CAAGGTGATT GGCCGAATGT GTAAGATCAT CGACAAGACG
    TGTCTGTCTC CCACTCCGAC CCTTGAGCAG CACCTCATGT GGGACGACAT CGCCATCCTG
    GCCCGGTACA TGCTCATGCT GTCCTTCAAC AACTCTCTGG ACGTGGCCGC TCATCTGCCA
    TACCTGTTCC ACGTCGTCAC CCTGCTGGTG GCCACGGGCC CTCTCTCGCT GCGTGCATCA
    ACACACGGCC TGGTCATCAA CATCATTCAC TCGCTCTGCA CATGCTCACA ACTCAACTTC
    AGTGAGGAGA CTAAACAGGT GCTGCGCCTG AGTCTGACTG AGTTCTCCCT GCCCAAGTTC
    TACCTGCTGT TCGGCATCAG TAAGGTCAAA TCTGCGGCGG TCATCGCTTT CCGTTCCAGC
    TACAGAGACC GCTCATTCTC TCCAGGCTCT TATGAGAGAG AGACATTTGC TCTGTCTTCC
    CTGGAAACAG TCACGGAGGC TCTTTTGGAG ATCATGGAGG CCTGCATGAG GGACATTCCT
    GGATGCAAAT GGCTAGATCA GTGGACTGAA TTGGCGCAAA AGTTTGCGTT CCAGTATAAC
    CCCTCTCTTC AGCCTCGGGC GCTGGTGGTG TTCGGCTGCA TCAGTAAACG AGTGACTCAC
    GGACAGCTAA AGCAGATCAT TCGTATTCTC AGTAAAGCCA GTCCTCTACT CAGCATTGAT
    CGGGGGCTGG AGAGCTGCTT AAAGGGCCCA GATAATTACA ACAGCCAGGT TCTCATCGAA
    GCCACAGTCA TCGCTCTCAC CAAACTACAG CCTCTCCTCG ATCAGGATTC TCCTATGCAT
    AAAGCTCTGT TCTGGGTGGC CGTGGCCGTG CTTCAGTTGG ATGAAGTGAA TCTGTACTCC
    GCCGGCACTG CCTTACTGGA GCAGAACCTG CACACACTGG ACAGCCTCCG AGTGTTTAAT
    GATAAGAGTC CTGAAGAGGT TTTTATGGAG ATCCGGAAAC CTCTAGAGTG GCACTGTAAA
    CAAATGGACC ATTTTGTGGG ACTCAACTTT GCCTCCAACT TCAATTTTGC ATTAGTGGGC
    CATTTACTTA AAGGATACAG ACATCCCTCT TCAACAACAG TTGCCAGGAC AGTTCGTATC
    CTGCATACAC TCCTGGGCCT GGTGGGAAAA CACCGCAGCT GTGACAAGTT TGAGGTGAAC
    ACGCAGAGTG TGGCCTATCT GGCAGCTTTG CTCACAGTGT CAGAGGAAGT TAGGAGCCGC
    TGCAGTTTAA AGCACAGGAA ATCATTGCTG CTTTCAGAAG TTACAATGGA GAACTTCCCC
    ATGGACACAT ACACCGTTTA CGACACTGAA CCCGGGTGCA GGCTACTGCG TGAAAACCAG
    CCTTGGGCTT CTCCGAAAGT GTCAGAGCGC AGTCTTGCAC CTCCGTACTC CACAATAGGG
    CAGACCAGCC TTCGTCCACG CAAATCCATG AGTGTAGACA TGGGCCAGCC GTCTCAGGCC
    AATGCGAAGA AACTACTGGG CACTAGAAAG AGTTTCGATC ACTTAATATC AGAATCCAAA
    GCCCCAAAAA GACCAGACAT GGAGTCGGGC ATCACCACAC CTCCAAAGAT GAGGCGGGTT
    TCTGAAAATG ACTATGAGAT CGAAACTCAG CGAATAGCCA ACTCTCATTT ACGCAAAGTG
    TCTGTGTCTG AATCCAATGT CCTCCTGGAC GAGAACGTAC TTACGGACCC CAAGATCCAG
    ACGTTGCTCC TCACTGTACT GGCAACACTG GTGAAGTACA CTACAGACGA GTTTCATCAG
    CGGATCTTGT ATGAGTATTT GGCTGAGGCC AGTGTGGTCT TCCCCAAGGT CTTTCCAGTT
    GTGCACAACC TGCTGGACTC CAAGTTAAGC ACCGTCCTTT CCATGTGCCA GGACCCGAAC
    CTTCTGAACC CCGTCCATGG CATCATTCAG AACGTGGTCT ATCATGAGGA GTCTCCCCCT
    CAGTACCAGC CGTCCTGCTT GCAGAGCTTT GGTTTCAATG GTCTTTGGAG GTTTGCCGGG
    CCGTTCTCTA AGCAAACCCA AATTCCAGAC TACGCCGAGC TGGTGGTTAA ATTTTTGGAT
    GCTTTGATTG ACACGTATCT CCCGGGCATT GAGGAGGAAG GAGGAGAAGA GTCACTGCGG
    ACCCCGACCT CGCCATATCC ACCAACCGTC CAGAGCCAGC TGAGCATCAC AGCCAATCTC
    AACCTGTCCA ACTCCATGAC ATCACTTGCC ACATCACAGC ACTCCCCAGG CATCGACAAG
    GAGAACGTTG AGATTTCTCC CACCTCGAGT CTGTCCACCA GTGGGCGAAT GCGGCACGGC
    TCAGCAAGCC AGGTGCAGAA GCAGCGCAGC GCAGGCAGCT TCAAACGGCC AAGCATCAAA
    AAGATCGTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa39878
    Clone ID Related Accession (Same CDS sequence) XM_009291769.2 , XM_009291769.3
    Accession Version XM_009291769.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8292bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product neurofibromin isoform X8
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009291769.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    ATGGCGGCGC ACAAGCCCGT GGAATGGGTC CAGGCCATCA TTAACAGATT CGACGAACAG 
    CTCCCAATCA AAACAGGACA GCAGAGCACC CACACCAAAG TGAGTACAGA GCACAACAAA
    GAATGTTTGA TCAACATCTC CAAGTACAAA TTTGCCCTCG TCATCAATGG CCTGACCAAC
    ATACTCAAAA ATGTGAACAA CATGCGGATA TTTGGCGAAG CTGCTGAGAA GAACCTCTAC
    TTCTCCCAGC TGATCATACT GGACACACTG GAGAAGTGTC TAGCAGGGCA ACCTAAGGAT
    TGCCTGAGGC TGGATGAGGC TATGTTGGTA AAACAGTTGC TGCCTGAAAT CTGTCACTTC
    CTGCACTCGT ACCGTGAGGG CCAGCAACAC GCCACTCAGC TGCGCAGCTC CGCCTCTGCT
    GTGCTTTTCT CTCTCAGCTG CAACAACTTT AATGCCGTCT TCAGTCGTAT CTCCACCAGG
    TTGCAAGAGC TCACAGTTTG TACAGAGGAC ACAGTGGATG TCCATGACAT TGAGCTCATT
    CAGTATATCA ATGTAGATTG CTCCAAGTTA AAGAGACTCC TACAAGAAGC AGTACTTAAG
    TTCAAGTCTT TGAAAAAGCC GGCTCAGCTT GCAGTAATCA GCAGTTTGGA AAAGGCTTTC
    TGGAACTGGG TTGAACACTA CCCTGATGAT TTCACTATGC TCTATCAGAG ACCCCAGGCC
    GACATGGCCG ACTGTGCAGA GAGACTGTTC GACCTAGTGG ACAGTTTTGC GGAAAGCGCA
    AAGAGAAAAG CTGCTGTGTG GCCACTGCAG ATCATCCTCC TCATCCTCTG TCCAGAGATC
    ACGCAGAGCA TCACCAAAGA AGTAGGGGGA GAGGAGAAAG CTAACAAGAA GTCATTTGTA
    GACAGTTTAC GAAAGGCTCT TGGCCAACAC AGCGGCAGCA AACAATTGAC GGAAAGTGCA
    GCTGTTGCAT GCGTCAAACT CTGCAAAGCC TGCACATATA TCAACTGGGA GGATAATTCT
    GTCATCTTTC TCCTGGTTCA GTCCATCGTC ATGGACCTTA AGGGTCTCCT GTTTAACCCC
    ACCAAACCTT TCTCAAGAGG TACGGGCTGC CAGAGCGCCG ATGTAGATTT GATGATCGAC
    TGCTTTGTGT CATGTTATCG CATCAATCCT CGCAACAACC AGCATTTTAA GGTGTGTCTG
    GCCTCGACTT CTCCTCCTAC CTTCCATTTC GTCCTTGTCA ACTCCCTGCA CAAAATTATC
    ACAAATTCTC ACCTGGACTG GTGGCCGAAG ATTGACGCCA TCTACTGCTA CTCAGGAGAA
    CTACGCACCA TGTTTGCAGA CACACTGGGT CGAGTGATTC AGAGTCTCAG TGCACATCCG
    CCTCTGAGGA TGACATCAAG TTTGACATTC AAAGAGAAAA TGACAACAAG TTTGAAGTTC
    AAGGAAAAGT CCACAGATCT TGATTCGCGT TCTTTGCTGC TGGCACTGGT CAAACTTATC
    CACGCTGACC CCAAACTAAT GCTGCACAAT CCGGGAAAAC CTGGTCAGGA GATTCAGAAC
    AGCACAGCTG AGATCATCAA AGGTCTGGTT CAGCTGGTTC CTCTTACCCA CAGTGCTGAG
    CTTTCCCAAG AGGCCATGGA GGCATTGCTT GTTTTACATC AGCCAGAAAC CATTGAGCTA
    TGGAACCCGG ACGCTCCCAT TGAGACCTTC TGGGACATCA GCTCTCAGGT GTTATTTCTA
    ATTTGTAAGA AGCTGATTGG ACACCAGATG ATCAACAGTA CAGAGATACT CAAGTGGCTC
    AGGGAGATTC TCATATGCAG GAACAAGTTT CTATTAAAGA ACAAGGAGTG TGCTACACAG
    GGCAGTCTGA TCCCCATCAG CAGACAGGCT CAGACCAAGC TGGAGGTGTG TCTGTACACG
    TTCCTGTGGA GTCCTGATGC AGAGGCTGTT TTGGTGGCCA TGTCCTGTTT CAGGCATCTC
    TGTGAGGAGG CCGATATTCG CTGCAGTGTC GATGAGCTGC CTGTTCACAC AGTCCTACCC
    AATTACAACA CCTTCATTGA GCTTGCATCC GTCAGCAATA TGATGGCCAC AGGTCGCAAC
    ACACTACAAA AAAGAGTGAT GGCACTTTTA AGAAGAATAA AGCACCCTAC AGCTGGAAAT
    GTAGAGGCAT GGGAAGACAC ATATGCAAAG TGGGAGCAAG CTACAAAACA GATACTCAAC
    CACCCAAAAA ACAAAGTGGA AGATGGACAG GTAGTTAACA TAAGATTGGT CAATTATTTC
    ATCTTTTTTT CCATTCATGG TCATCTATTT TCTGTTCGTC CTCAGGAGTG GATCAACATG
    ACTGGTTTCT TGTGCGCTCT GGGAGGTGTT TGTCTCCAAC AGCGCAGCAC GCCGAGTCTG
    GGCACCTACA GTCCTCCAAT GGGCCCCCTG ACCGAACGCA AGGGGTCCAT GATTTCCATG
    AGCTCCTGCG AGGTAAACAG CGAAACATCC CTGAATCGCT TTCTGGAGCG TCTGCTGGCT
    CTGATGGTCT GCACACATGA GAAAGTGGGA GTGCAAATTC GCACCAACGT CAAAGACCTG
    GTGGGACTGG AGCTCAGTCC GGCTCTCTAT TCAATGCTCT TCACTAAGCT GCGCAACAGC
    ATCGGACGCT TCTTTGACAC CCAGGGGCCG GTTCCTATTA CAGATGCAAA CACTCTGTTT
    GTAGAGCAGA CCATTGCTAT CATGAAGAAC TTGCTGGACA GTCACACTGA AGGAAGCTCA
    GAGCACCTTG GTCAAGCCAG CATTGAGACC ATGATGCTCA ATCTAGTTAG GTATGTGCGT
    ATTTTAGGGA ACATTGTTCA TGCCATTCAG ATCAAAACCA AGTTGTGTCA GTTAGTGGAG
    GTGATGATGG AGCGCAGGGA TGACCTCTCC TTCTGCCAGG AGATGAAATT CAGGAATAAA
    ATGGTGGAGT ACCTCACAGA CTGGGTGATG GGAACCTCCA ATCAAGCTGC GGACGATGAT
    GTCAAATGCC TGACCCGCGA TCTGGACCAG GCCAGTATGG AGGCTGTCGT GTCTCTGTTG
    GCTGGTCTTC CTCTACAGCC TGAGGAGGGT GACGGTGTTG AGCTCATGGA GGCCAAATCA
    CAGCTCTTCC TGAAGTACTT CACACTGTTC ATGAACCTGT TGAATGACTG CAGCGAGGTT
    GAGGAAGATG GCCAGCAGGT CGCAGGGAGG AAACGTGGCA TGTCTAGACG ACTGGCATCT
    CTGCGGCACT GCACAGTATT GGCTATGTCT AACTTGCTCA ATGCTAATGT GGATAGCGGC
    CTCATGCACT CAATCGGTCT GGGATACCAT AAGGACCTGC AGACACGGGC TACATTTATG
    GAAGTGCTCA CTAAGATCCT TCAGCAAGGG ACGGAGTTCG ACACGCTTGC AGAGACTGTG
    CTTGCAGATC GCTTTGAGAG ACTGGTCGAG CTGGTCACCA TGATGGGAGA TCAGGGAGAG
    CTGCCCATAG CCATGGCTCT GGCCAATGTG GTCCCATGCT CTCAATGGGA TGAGCTGGCC
    AGAGTGTTAG TGACTCTCTT CGACTCTCGC CATCTGCTGT ATCAGCTGTT GTGGAATATG
    TTCTCCAAAG AGGTGGAGCT GGCAGACTCA ATGCAGACGC TCTTTAGAGG AAATAGCCTA
    GCCAGCAAGA TTATGACCTT CTGCTTCAAG GTGTACGGAG CTGCGTATCT ACAGAAGCTT
    CTGGAGCCGC TGTTGAGAGG AATCATCACT ACACCAGAGT GGCAGCAGAT CAGTTTTGAA
    GTGGACCCTA CTAGACTTGA ACAGGCAGAG AACCTGGAGG AGAACCAGAA GAACCTGCTT
    CAGATCACTG ACCGCTTCTT CCTGGCCATC ATCAACTCCT CGAGCGAGTT TCCTCCACAG
    CTGCGCAGTG TGTGCCACTG TCTGTATCAG GTGGTCAGTC AGCGGTTTCC TCAGAACAGC
    ATTGGTGCTG TAGGAAGTGC CATGTTCCTG CGCTTTATTA ACCCAGCTAT CGTGTCCCCA
    TATGAGGCTG GAATCCTAGA CAAGAAACCC CCACCTCGCA TCGAGCGTGG CCTTAAACTC
    ATGTCTAAGA TTCTGCAGAG CATTGCTAAT CATGTTCTCT TCACAAAAGA GGAGCATATG
    AGGCCCTTTA ATGATTTTGT CAAAAGCAAC TTCGATGCAG CCAGAAGGTT CTTCTTGGAT
    ATCGCATCTG ATTCTCCACC AAGTGATTCT GTGAACCATA GTCTGTCTTT CATCAGTGAC
    GGTAATGTGC TCGCTCTGCA CCGGCTATTA TGGAACAACC AGGAAAGAAT TGGCCAATAC
    CTGTCCAGCA ACAGGGACCA CAAGGCAGTG GGCCGTAGAC CATTTGACAA GATGGCCACT
    CTTCTGGCGT ACCTGGGTCC TCCTGAACAT AAGCCAGTAG CAGACACACA CTGGTCCAGC
    CTCAACCTCA CCAGCTCAAA GTTTGAGGAG TTCATGACAA GGCATCAAGT CCATGAGAAG
    GAGGAATTTA AAGCATTAAA AACCCTCAAC ATATTTTACC AAGCTGGCAC ATCTAAGAAC
    GGCAACCCTG TGTTTTATTA CATCGCACGC AGGTTCAAAA CTGGACAAAT CAATGGTGAT
    CTTCTGATCT ACCACGTCCT GCTGACGCTC AAGCCCTACT ATGCCAAGCA TTATGAGATC
    GTAGTTGACC TTACTCATGT GGGCCCCAGT AACCGTTTCA AGACGGACTT CTTATCCAAA
    TGGTTTGTGG TCTTTCCAAA CTTCGCCTAT GAGAACGTGG CTGCTGTGTA CATCTACAAC
    TGCAACACCT GGGTACGCGA GTACACCAAA TACCACGAGC GACTGCTGAC TGGCCTCAAG
    GGGAGCAAGA AGCTGGTGTT TATCGATTCG CCTGCACGTC TAGCTGAACA CATCGAACCC
    GAACAGCAGA AGCTGCCCGC AGCTACTTTA GCACTGGAAG AAGACCTCAA AGTGTTCCAC
    AACGCCCTAA AGCTTGCACA CAGAGACACA AAAGTGTCAA TTAAGGTGGG ATCCACAGCT
    GTACAGGTGA CCTCGGCCGA AAGAACGCGT GTCCTAGGAC AGCCTGTGTT CCTAAATGAC
    ATCTACTACG CTTCAGAGAT CGAGGAGATA TGCCTGGTGG ACGAGAACCA GTTCACGCTA
    ACCATCGCTA ACCAGGGCAC CCCACTCACT TTCATGCACC AGGAGTGTGA AGCCATCGTT
    CAGTCCATCA TCCACATCCG CACGCGCTGG GAACTTTCCC AACCAGACTC CATTCCCCAA
    CACACCAAAA TCAGGCCTAA GGATGTTCCT GGCACCCTGC TCAACATCGC TCTTCTCAAC
    CTGGGAAGCT CCGACCCCAG TCTGAGGTCT GCGGCCTACA ATCTCCTTTG TGCCTTAACC
    TGCACCTTCA ACCTGAAGAT TGAAGGCCAG CTTCTGGAAA CGTCTGGCTT GTGCATCCCA
    GCCAACAACA CGCTTTTTAT TGTGTCCATT AGCAAAACGC TAGCAGCTAA TGAACCCCAC
    CTCACTCTGG AGTTCCTGGA AGAGTGCATC TCAGGATTCA GTAAATCAAG TATAGAGCTG
    AAGCATCTGT GTCTAGAATA TATGACTCCT TGGCTCCGTA ATTTGGTGCG CTTTTGCAAA
    CACAATGATG ACGCCAAACG GCAGCGTGTC ACGGCCATCC TGGACAAGCT TATTACTATG
    ACCATCAACG AGAAGCAGAT GTACCCCTCC ATACAGGCCA AGATATGGGG CAGCTTGGGT
    CAGATCACAG ACCTGCTTGA TGTGGTGTTG GACAGCTTTA TAAAGACCAG CGCTACTGGC
    GGTTTAGGAT CCATCAAAGC TGAAGTGATG GCTGACACTG CTGTAGCTTT GGCGTCAGGA
    AATGTCAAAC TGGTCTCCAG CAAGGTGATT GGCCGAATGT GTAAGATCAT CGACAAGACG
    TGTCTGTCTC CCACTCCGAC CCTTGAGCAG CACCTCATGT GGGACGACAT CGCCATCCTG
    GCCCGGTACA TGCTCATGCT GTCCTTCAAC AACTCTCTGG ACGTGGCCGC TCATCTGCCA
    TACCTGTTCC ACGTCGTCAC CCTGCTGGTG GCCACGGGCC CTCTCTCGCT GCGTGCATCA
    ACACACGGCC TGGTCATCAA CATCATTCAC TCGCTCTGCA CATGCTCACA ACTCAACTTC
    AGTGAGGAGA CTAAACAGGT GCTGCGCCTG AGTCTGACTG AGTTCTCCCT GCCCAAGTTC
    TACCTGCTGT TCGGCATCAG TAAGGTCAAA TCTGCGGCGG TCATCGCTTT CCGTTCCAGC
    TACAGAGACC GCTCATTCTC TCCAGGCTCT TATGAGAGAG AGACATTTGC TCTGTCTTCC
    CTGGAAACAG TCACGGAGGC TCTTTTGGAG ATCATGGAGG CCTGCATGAG GGACATTCCT
    GGATGCAAAT GGCTAGATCA GTGGACTGAA TTGGCGCAAA AGTTTGCGTT CCAGTATAAC
    CCCTCTCTTC AGCCTCGGGC GCTGGTGGTG TTCGGCTGCA TCAGTAAACG AGTGACTCAC
    GGACAGCTAA AGCAGATCAT TCGTATTCTC AGTAAAGCCA GTCCTCTACT CAGCATTGAT
    CGGGGGCTGG AGAGCTGCTT AAAGGGCCCA GATAATTACA ACAGCCAGGT TCTCATCGAA
    GCCACAGTCA TCGCTCTCAC CAAACTACAG CCTCTCCTCG ATCAGGATTC TCCTATGCAT
    AAAGCTCTGT TCTGGGTGGC CGTGGCCGTG CTTCAGTTGG ATGAAGTGAA TCTGTACTCC
    GCCGGCACTG CCTTACTGGA GCAGAACCTG CACACACTGG ACAGCCTCCG AGTGTTTAAT
    GATAAGAGTC CTGAAGAGGT TTTTATGGAG ATCCGGAAAC CTCTAGAGTG GCACTGTAAA
    CAAATGGACC ATTTTGTGGG ACTCAACTTT GCCTCCAACT TCAATTTTGC ATTAGTGGGC
    CATTTACTTA AAGGATACAG ACATCCCTCT TCAACAACAG TTGCCAGGAC AGTTCGTATC
    CTGCATACAC TCCTGGGCCT GGTGGGAAAA CACCGCAGCT GTGACAAGTT TGAGGTGAAC
    ACGCAGAGTG TGGCCTATCT GGCAGCTTTG CTCACAGTGT CAGAGGAAGT TAGGAGCCGC
    TGCAGTTTAA AGCACAGGAA ATCATTGCTG CTTTCAGAAG TTACAATGGA GAACTTCCCC
    ATGGACACAT ACACCGTTTA CGACACTGAA CCCGGGTGCA GGCTACTGCG TGAAAACCAG
    CCTTGGGCTT CTCCGAAAGT GTCAGAGCGC AGTCTTGCAC CTCCGTACTC CACAATAGGG
    CAGACCAGCC TTCGTCCACG CAAATCCATG AGTGTAGACA TGGGCCAGCC GTCTCAGGCC
    AATGCGAAGA AACTACTGGG CACTAGAAAG AGTTTCGATC ACTTAATATC AGAATCCAAA
    GCCCCAAAAA GACCAGACAT GGAGTCGGGC ATCACCACAC CTCCAAAGAT GAGGCGGGTT
    TCTGAAAATG ACTATGAGAT CGAAACTCAG CGAATAGCCA ACTCTCATTT ACGCAAAGTG
    TCTGTGTCTG AATCCAATGT CCTCCTGGAC GAGAACGTAC TTACGGACCC CAAGATCCAG
    ACGTTGCTCC TCACTGTACT GGCAACACTG GTGAAGTACA CTACAGACGA GTTTCATCAG
    CGGATCTTGT ATGAGTATTT GGCTGAGGCC AGTGTGGTCT TCCCCAAGGT CTTTCCAGTT
    GTGCACAACC TGCTGGACTC CAAGTTAAGC ACCGTCCTTT CCATGTGCCA GGACCCGAAC
    CTTCTGAACC CCGTCCATGG CATCATTCAG AACGTGGTCT ATCATGAGGA GTCTCCCCCT
    CAGTACCAGC CGTCCTGCTT GCAGAGCTTT GGTTTCAATG GTCTTTGGAG GTTTGCCGGG
    CCGTTCTCTA AGCAAACCCA AATTCCAGAC TACGCCGAGC TGGTGGTTAA ATTTTTGGAT
    GCTTTGATTG ACACGTATCT CCCGGGCATT GAGGAGGAAG GAGGAGAAGA GTCACTGCGG
    ACCCCGACCT CGCCATATCC ACCAACCGTC CAGAGCCAGC TGAGCATCAC AGCCAATCTC
    AACCTGTCCA ACTCCATGAC ATCACTTGCC ACATCACAGC ACTCCCCAGG CATCGACAAG
    GAGAACGTTG AGATTTCTCC CACCTCGAGT CTGTCCACCA GTGGGCGAAT GCGGCACGGC
    TCAGCAAGCC AGGTGCAGAA GCAGCGCAGC GCAGGCAGCT TCAAACGGCC AAGCATCAAA
    AAGATCGTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009290044.1
    CDS247..8538
    Translation

    Target ORF information:

    RefSeq Version XM_009291769.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio neurofibromin 1a (nf1a), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009291769.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGGCGGCGC ACAAGCCCGT GGAATGGGTC CAGGCCATCA TTAACAGATT CGACGAACAG 
    CTCCCAATCA AAACAGGACA GCAGAGCACC CACACCAAAG TGAGTACAGA GCACAACAAA
    GAATGTTTGA TCAACATCTC CAAGTACAAA TTTGCCCTCG TCATCAATGG CCTGACCAAC
    ATACTCAAAA ATGTGAACAA CATGCGGATA TTTGGCGAAG CTGCTGAGAA GAACCTCTAC
    TTCTCCCAGC TGATCATACT GGACACACTG GAGAAGTGTC TAGCAGGGCA ACCTAAGGAT
    TGCCTGAGGC TGGATGAGGC TATGTTGGTA AAACAGTTGC TGCCTGAAAT CTGTCACTTC
    CTGCACTCGT ACCGTGAGGG CCAGCAACAC GCCACTCAGC TGCGCAGCTC CGCCTCTGCT
    GTGCTTTTCT CTCTCAGCTG CAACAACTTT AATGCCGTCT TCAGTCGTAT CTCCACCAGG
    TTGCAAGAGC TCACAGTTTG TACAGAGGAC ACAGTGGATG TCCATGACAT TGAGCTCATT
    CAGTATATCA ATGTAGATTG CTCCAAGTTA AAGAGACTCC TACAAGAAGC AGTACTTAAG
    TTCAAGTCTT TGAAAAAGCC GGCTCAGCTT GCAGTAATCA GCAGTTTGGA AAAGGCTTTC
    TGGAACTGGG TTGAACACTA CCCTGATGAT TTCACTATGC TCTATCAGAG ACCCCAGGCC
    GACATGGCCG ACTGTGCAGA GAGACTGTTC GACCTAGTGG ACAGTTTTGC GGAAAGCGCA
    AAGAGAAAAG CTGCTGTGTG GCCACTGCAG ATCATCCTCC TCATCCTCTG TCCAGAGATC
    ACGCAGAGCA TCACCAAAGA AGTAGGGGGA GAGGAGAAAG CTAACAAGAA GTCATTTGTA
    GACAGTTTAC GAAAGGCTCT TGGCCAACAC AGCGGCAGCA AACAATTGAC GGAAAGTGCA
    GCTGTTGCAT GCGTCAAACT CTGCAAAGCC TGCACATATA TCAACTGGGA GGATAATTCT
    GTCATCTTTC TCCTGGTTCA GTCCATCGTC ATGGACCTTA AGGGTCTCCT GTTTAACCCC
    ACCAAACCTT TCTCAAGAGG TACGGGCTGC CAGAGCGCCG ATGTAGATTT GATGATCGAC
    TGCTTTGTGT CATGTTATCG CATCAATCCT CGCAACAACC AGCATTTTAA GGTGTGTCTG
    GCCTCGACTT CTCCTCCTAC CTTCCATTTC GTCCTTGTCA ACTCCCTGCA CAAAATTATC
    ACAAATTCTC ACCTGGACTG GTGGCCGAAG ATTGACGCCA TCTACTGCTA CTCAGGAGAA
    CTACGCACCA TGTTTGCAGA CACACTGGGT CGAGTGATTC AGAGTCTCAG TGCACATCCG
    CCTCTGAGGA TGACATCAAG TTTGACATTC AAAGAGAAAA TGACAACAAG TTTGAAGTTC
    AAGGAAAAGT CCACAGATCT TGATTCGCGT TCTTTGCTGC TGGCACTGGT CAAACTTATC
    CACGCTGACC CCAAACTAAT GCTGCACAAT CCGGGAAAAC CTGGTCAGGA GATTCAGAAC
    AGCACAGCTG AGATCATCAA AGGTCTGGTT CAGCTGGTTC CTCTTACCCA CAGTGCTGAG
    CTTTCCCAAG AGGCCATGGA GGCATTGCTT GTTTTACATC AGCCAGAAAC CATTGAGCTA
    TGGAACCCGG ACGCTCCCAT TGAGACCTTC TGGGACATCA GCTCTCAGGT GTTATTTCTA
    ATTTGTAAGA AGCTGATTGG ACACCAGATG ATCAACAGTA CAGAGATACT CAAGTGGCTC
    AGGGAGATTC TCATATGCAG GAACAAGTTT CTATTAAAGA ACAAGGAGTG TGCTACACAG
    GGCAGTCTGA TCCCCATCAG CAGACAGGCT CAGACCAAGC TGGAGGTGTG TCTGTACACG
    TTCCTGTGGA GTCCTGATGC AGAGGCTGTT TTGGTGGCCA TGTCCTGTTT CAGGCATCTC
    TGTGAGGAGG CCGATATTCG CTGCAGTGTC GATGAGCTGC CTGTTCACAC AGTCCTACCC
    AATTACAACA CCTTCATTGA GCTTGCATCC GTCAGCAATA TGATGGCCAC AGGTCGCAAC
    ACACTACAAA AAAGAGTGAT GGCACTTTTA AGAAGAATAA AGCACCCTAC AGCTGGAAAT
    GTAGAGGCAT GGGAAGACAC ATATGCAAAG TGGGAGCAAG CTACAAAACA GATACTCAAC
    CACCCAAAAA ACAAAGTGGA AGATGGACAG GTAGTTAACA TAAGATTGGT CAATTATTTC
    ATCTTTTTTT CCATTCATGG TCATCTATTT TCTGTTCGTC CTCAGGAGTG GATCAACATG
    ACTGGTTTCT TGTGCGCTCT GGGAGGTGTT TGTCTCCAAC AGCGCAGCAC GCCGAGTCTG
    GGCACCTACA GTCCTCCAAT GGGCCCCCTG ACCGAACGCA AGGGGTCCAT GATTTCCATG
    AGCTCCTGCG AGGTAAACAG CGAAACATCC CTGAATCGCT TTCTGGAGCG TCTGCTGGCT
    CTGATGGTCT GCACACATGA GAAAGTGGGA GTGCAAATTC GCACCAACGT CAAAGACCTG
    GTGGGACTGG AGCTCAGTCC GGCTCTCTAT TCAATGCTCT TCACTAAGCT GCGCAACAGC
    ATCGGACGCT TCTTTGACAC CCAGGGGCCG GTTCCTATTA CAGATGCAAA CACTCTGTTT
    GTAGAGCAGA CCATTGCTAT CATGAAGAAC TTGCTGGACA GTCACACTGA AGGAAGCTCA
    GAGCACCTTG GTCAAGCCAG CATTGAGACC ATGATGCTCA ATCTAGTTAG GTATGTGCGT
    ATTTTAGGGA ACATTGTTCA TGCCATTCAG ATCAAAACCA AGTTGTGTCA GTTAGTGGAG
    GTGATGATGG AGCGCAGGGA TGACCTCTCC TTCTGCCAGG AGATGAAATT CAGGAATAAA
    ATGGTGGAGT ACCTCACAGA CTGGGTGATG GGAACCTCCA ATCAAGCTGC GGACGATGAT
    GTCAAATGCC TGACCCGCGA TCTGGACCAG GCCAGTATGG AGGCTGTCGT GTCTCTGTTG
    GCTGGTCTTC CTCTACAGCC TGAGGAGGGT GACGGTGTTG AGCTCATGGA GGCCAAATCA
    CAGCTCTTCC TGAAGTACTT CACACTGTTC ATGAACCTGT TGAATGACTG CAGCGAGGTT
    GAGGAAGATG GCCAGCAGGT CGCAGGGAGG AAACGTGGCA TGTCTAGACG ACTGGCATCT
    CTGCGGCACT GCACAGTATT GGCTATGTCT AACTTGCTCA ATGCTAATGT GGATAGCGGC
    CTCATGCACT CAATCGGTCT GGGATACCAT AAGGACCTGC AGACACGGGC TACATTTATG
    GAAGTGCTCA CTAAGATCCT TCAGCAAGGG ACGGAGTTCG ACACGCTTGC AGAGACTGTG
    CTTGCAGATC GCTTTGAGAG ACTGGTCGAG CTGGTCACCA TGATGGGAGA TCAGGGAGAG
    CTGCCCATAG CCATGGCTCT GGCCAATGTG GTCCCATGCT CTCAATGGGA TGAGCTGGCC
    AGAGTGTTAG TGACTCTCTT CGACTCTCGC CATCTGCTGT ATCAGCTGTT GTGGAATATG
    TTCTCCAAAG AGGTGGAGCT GGCAGACTCA ATGCAGACGC TCTTTAGAGG AAATAGCCTA
    GCCAGCAAGA TTATGACCTT CTGCTTCAAG GTGTACGGAG CTGCGTATCT ACAGAAGCTT
    CTGGAGCCGC TGTTGAGAGG AATCATCACT ACACCAGAGT GGCAGCAGAT CAGTTTTGAA
    GTGGACCCTA CTAGACTTGA ACAGGCAGAG AACCTGGAGG AGAACCAGAA GAACCTGCTT
    CAGATCACTG ACCGCTTCTT CCTGGCCATC ATCAACTCCT CGAGCGAGTT TCCTCCACAG
    CTGCGCAGTG TGTGCCACTG TCTGTATCAG GTGGTCAGTC AGCGGTTTCC TCAGAACAGC
    ATTGGTGCTG TAGGAAGTGC CATGTTCCTG CGCTTTATTA ACCCAGCTAT CGTGTCCCCA
    TATGAGGCTG GAATCCTAGA CAAGAAACCC CCACCTCGCA TCGAGCGTGG CCTTAAACTC
    ATGTCTAAGA TTCTGCAGAG CATTGCTAAT CATGTTCTCT TCACAAAAGA GGAGCATATG
    AGGCCCTTTA ATGATTTTGT CAAAAGCAAC TTCGATGCAG CCAGAAGGTT CTTCTTGGAT
    ATCGCATCTG ATTCTCCACC AAGTGATTCT GTGAACCATA GTCTGTCTTT CATCAGTGAC
    GGTAATGTGC TCGCTCTGCA CCGGCTATTA TGGAACAACC AGGAAAGAAT TGGCCAATAC
    CTGTCCAGCA ACAGGGACCA CAAGGCAGTG GGCCGTAGAC CATTTGACAA GATGGCCACT
    CTTCTGGCGT ACCTGGGTCC TCCTGAACAT AAGCCAGTAG CAGACACACA CTGGTCCAGC
    CTCAACCTCA CCAGCTCAAA GTTTGAGGAG TTCATGACAA GGCATCAAGT CCATGAGAAG
    GAGGAATTTA AAGCATTAAA AACCCTCAAC ATATTTTACC AAGCTGGCAC ATCTAAGAAC
    GGCAACCCTG TGTTTTATTA CATCGCACGC AGGTTCAAAA CTGGACAAAT CAATGGTGAT
    CTTCTGATCT ACCACGTCCT GCTGACGCTC AAGCCCTACT ATGCCAAGCA TTATGAGATC
    GTAGTTGACC TTACTCATGT GGGCCCCAGT AACCGTTTCA AGACGGACTT CTTATCCAAA
    TGGTTTGTGG TCTTTCCAAA CTTCGCCTAT GAGAACGTGG CTGCTGTGTA CATCTACAAC
    TGCAACACCT GGGTACGCGA GTACACCAAA TACCACGAGC GACTGCTGAC TGGCCTCAAG
    GGGAGCAAGA AGCTGGTGTT TATCGATTCG CCTGCACGTC TAGCTGAACA CATCGAACCC
    GAACAGCAGA AGCTGCCCGC AGCTACTTTA GCACTGGAAG AAGACCTCAA AGTGTTCCAC
    AACGCCCTAA AGCTTGCACA CAGAGACACA AAAGTGTCAA TTAAGGTGGG ATCCACAGCT
    GTACAGGTGA CCTCGGCCGA AAGAACGCGT GTCCTAGGAC AGCCTGTGTT CCTAAATGAC
    ATCTACTACG CTTCAGAGAT CGAGGAGATA TGCCTGGTGG ACGAGAACCA GTTCACGCTA
    ACCATCGCTA ACCAGGGCAC CCCACTCACT TTCATGCACC AGGAGTGTGA AGCCATCGTT
    CAGTCCATCA TCCACATCCG CACGCGCTGG GAACTTTCCC AACCAGACTC CATTCCCCAA
    CACACCAAAA TCAGGCCTAA GGATGTTCCT GGCACCCTGC TCAACATCGC TCTTCTCAAC
    CTGGGAAGCT CCGACCCCAG TCTGAGGTCT GCGGCCTACA ATCTCCTTTG TGCCTTAACC
    TGCACCTTCA ACCTGAAGAT TGAAGGCCAG CTTCTGGAAA CGTCTGGCTT GTGCATCCCA
    GCCAACAACA CGCTTTTTAT TGTGTCCATT AGCAAAACGC TAGCAGCTAA TGAACCCCAC
    CTCACTCTGG AGTTCCTGGA AGAGTGCATC TCAGGATTCA GTAAATCAAG TATAGAGCTG
    AAGCATCTGT GTCTAGAATA TATGACTCCT TGGCTCCGTA ATTTGGTGCG CTTTTGCAAA
    CACAATGATG ACGCCAAACG GCAGCGTGTC ACGGCCATCC TGGACAAGCT TATTACTATG
    ACCATCAACG AGAAGCAGAT GTACCCCTCC ATACAGGCCA AGATATGGGG CAGCTTGGGT
    CAGATCACAG ACCTGCTTGA TGTGGTGTTG GACAGCTTTA TAAAGACCAG CGCTACTGGC
    GGTTTAGGAT CCATCAAAGC TGAAGTGATG GCTGACACTG CTGTAGCTTT GGCGTCAGGA
    AATGTCAAAC TGGTCTCCAG CAAGGTGATT GGCCGAATGT GTAAGATCAT CGACAAGACG
    TGTCTGTCTC CCACTCCGAC CCTTGAGCAG CACCTCATGT GGGACGACAT CGCCATCCTG
    GCCCGGTACA TGCTCATGCT GTCCTTCAAC AACTCTCTGG ACGTGGCCGC TCATCTGCCA
    TACCTGTTCC ACGTCGTCAC CCTGCTGGTG GCCACGGGCC CTCTCTCGCT GCGTGCATCA
    ACACACGGCC TGGTCATCAA CATCATTCAC TCGCTCTGCA CATGCTCACA ACTCAACTTC
    AGTGAGGAGA CTAAACAGGT GCTGCGCCTG AGTCTGACTG AGTTCTCCCT GCCCAAGTTC
    TACCTGCTGT TCGGCATCAG TAAGGTCAAA TCTGCGGCGG TCATCGCTTT CCGTTCCAGC
    TACAGAGACC GCTCATTCTC TCCAGGCTCT TATGAGAGAG AGACATTTGC TCTGTCTTCC
    CTGGAAACAG TCACGGAGGC TCTTTTGGAG ATCATGGAGG CCTGCATGAG GGACATTCCT
    GGATGCAAAT GGCTAGATCA GTGGACTGAA TTGGCGCAAA AGTTTGCGTT CCAGTATAAC
    CCCTCTCTTC AGCCTCGGGC GCTGGTGGTG TTCGGCTGCA TCAGTAAACG AGTGACTCAC
    GGACAGCTAA AGCAGATCAT TCGTATTCTC AGTAAAGCCA GTCCTCTACT CAGCATTGAT
    CGGGGGCTGG AGAGCTGCTT AAAGGGCCCA GATAATTACA ACAGCCAGGT TCTCATCGAA
    GCCACAGTCA TCGCTCTCAC CAAACTACAG CCTCTCCTCG ATCAGGATTC TCCTATGCAT
    AAAGCTCTGT TCTGGGTGGC CGTGGCCGTG CTTCAGTTGG ATGAAGTGAA TCTGTACTCC
    GCCGGCACTG CCTTACTGGA GCAGAACCTG CACACACTGG ACAGCCTCCG AGTGTTTAAT
    GATAAGAGTC CTGAAGAGGT TTTTATGGAG ATCCGGAAAC CTCTAGAGTG GCACTGTAAA
    CAAATGGACC ATTTTGTGGG ACTCAACTTT GCCTCCAACT TCAATTTTGC ATTAGTGGGC
    CATTTACTTA AAGGATACAG ACATCCCTCT TCAACAACAG TTGCCAGGAC AGTTCGTATC
    CTGCATACAC TCCTGGGCCT GGTGGGAAAA CACCGCAGCT GTGACAAGTT TGAGGTGAAC
    ACGCAGAGTG TGGCCTATCT GGCAGCTTTG CTCACAGTGT CAGAGGAAGT TAGGAGCCGC
    TGCAGTTTAA AGCACAGGAA ATCATTGCTG CTTTCAGAAG TTACAATGGA GAACTTCCCC
    ATGGACACAT ACACCGTTTA CGACACTGAA CCCGGGTGCA GGCTACTGCG TGAAAACCAG
    CCTTGGGCTT CTCCGAAAGT GTCAGAGCGC AGTCTTGCAC CTCCGTACTC CACAATAGGG
    CAGACCAGCC TTCGTCCACG CAAATCCATG AGTGTAGACA TGGGCCAGCC GTCTCAGGCC
    AATGCGAAGA AACTACTGGG CACTAGAAAG AGTTTCGATC ACTTAATATC AGAATCCAAA
    GCCCCAAAAA GACCAGACAT GGAGTCGGGC ATCACCACAC CTCCAAAGAT GAGGCGGGTT
    TCTGAAAATG ACTATGAGAT CGAAACTCAG CGAATAGCCA ACTCTCATTT ACGCAAAGTG
    TCTGTGTCTG AATCCAATGT CCTCCTGGAC GAGAACGTAC TTACGGACCC CAAGATCCAG
    ACGTTGCTCC TCACTGTACT GGCAACACTG GTGAAGTACA CTACAGACGA GTTTCATCAG
    CGGATCTTGT ATGAGTATTT GGCTGAGGCC AGTGTGGTCT TCCCCAAGGT CTTTCCAGTT
    GTGCACAACC TGCTGGACTC CAAGTTAAGC ACCGTCCTTT CCATGTGCCA GGACCCGAAC
    CTTCTGAACC CCGTCCATGG CATCATTCAG AACGTGGTCT ATCATGAGGA GTCTCCCCCT
    CAGTACCAGC CGTCCTGCTT GCAGAGCTTT GGTTTCAATG GTCTTTGGAG GTTTGCCGGG
    CCGTTCTCTA AGCAAACCCA AATTCCAGAC TACGCCGAGC TGGTGGTTAA ATTTTTGGAT
    GCTTTGATTG ACACGTATCT CCCGGGCATT GAGGAGGAAG GAGGAGAAGA GTCACTGCGG
    ACCCCGACCT CGCCATATCC ACCAACCGTC CAGAGCCAGC TGAGCATCAC AGCCAATCTC
    AACCTGTCCA ACTCCATGAC ATCACTTGCC ACATCACAGC ACTCCCCAGG CATCGACAAG
    GAGAACGTTG AGATTTCTCC CACCTCGAGT CTGTCCACCA GTGGGCGAAT GCGGCACGGC
    TCAGCAAGCC AGGTGCAGAA GCAGCGCAGC GCAGGCAGCT TCAAACGGCC AAGCATCAAA
    AAGATCGTCT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55268
    Clone ID Related Accession (Same CDS sequence) XM_017351191.1
    Accession Version XM_017351191.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8484bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product neurofibromin isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCGGCGC ACAAGCCCGT GGAATGGGTC CAGGCCATCA TTAACAGATT CGACGAACAG 
    CTCCCAATCA AAACAGGACA GCAGAGCACC CACACCAAAG TGAGTACAGA GCACAACAAA
    GAATGTTTGA TCAACATCTC CAAGTACAAA TTTGCCCTCG TCATCAATGG CCTGACCAAC
    ATACTCAAAA ATGTGAACAA CATGCGGATA TTTGGCGAAG CTGCTGAGAA GAACCTCTAC
    TTCTCCCAGC TGATCATACT GGACACACTG GAGAAGTGTC TAGCAGGGCA ACCTAAGGAT
    TGCCTGAGGC TGGATGAGGC TATGTTGGTA AAACAGTTGC TGCCTGAAAT CTGTCACTTC
    CTGCACTCGT ACCGTGAGGG CCAGCAACAC GCCACTCAGC TGCGCAGCTC CGCCTCTGCT
    GTGCTTTTCT CTCTCAGCTG CAACAACTTT AATGCCGTCT TCAGTCGTAT CTCCACCAGG
    TTGCAAGAGC TCACAGTTTG TACAGAGGAC ACAGTGGATG TCCATGACAT TGAGCTCATT
    CAGTATATCA ATGTAGATTG CTCCAAGTTA AAGAGACTCC TACAAGAAGC AGTACTTAAG
    TTCAAGTCTT TGAAAAAGCC GGCTCAGCTT GCAGTAATCA GCAGTTTGGA AAAGGCTTTC
    TGGAACTGGG TTGAACACTA CCCTGATGAT TTCACTATGC TCTATCAGAG ACCCCAGGCC
    GACATGGCCG ACTGTGCAGA GAGACTGTTC GACCTAGTGG ACAGTTTTGC GGAAAGCGCA
    AAGAGAAAAG CTGCTGTGTG GCCACTGCAG ATCATCCTCC TCATCCTCTG TCCAGAGATC
    ACGCAGAGCA TCACCAAAGA AGTAGGGGGA GAGGAGAAAG CTAACAAGAA GTCATTTGTA
    GACAGTTTAC GAAAGGCTCT TGGCCAACAC AGCGGCAGCA AACAATTGAC GGAAAGTGCA
    GCTGTTGCAT GCGTCAAACT CTGCAAAGCC TGCACATATA TCAACTGGGA GGATAATTCT
    GTCATCTTTC TCCTGGTTCA GTCCATCGTC ATGGACCTTA AGGGTCTCCT GTTTAACCCC
    ACCAAACCTT TCTCAAGAGG TACGGGCTGC CAGAGCGCCG ATGTAGATTT GATGATCGAC
    TGCTTTGTGT CATGTTATCG CATCAATCCT CGCAACAACC AGCATTTTAA GGTGTGTCTG
    GCCTCGACTT CTCCTCCTAC CTTCCATTTC GTCCTTGTCA ACTCCCTGCA CAAAATTATC
    ACAAATTCTC ACCTGGACTG GTGGCCGAAG ATTGACGCCA TCTACTGCTA CTCAGGAGAA
    CTACGCACCA TGTTTGCAGA CACACTGGGT CGAGTGATTC AGAGTCTCAG TGCACATCCG
    CCTCTGAGGA TGACATCAAG TTTGACATTC AAAGAGAAAA TGACAACAAG TTTGAAGTTC
    AAGGAAAAGT CCACAGATCT TGATTCGCGT TCTTTGCTGC TGGCACTGGT CAAACTTATC
    CACGCTGACC CCAAACTAAT GCTGCACAAT CCGGGAAAAC CTGGTCAGGA GATTCAGAAC
    AGCACAGCTG AGATCATCAA AGGTCTGGTT CAGCTGGTTC CTCTTACCCA CAGTGCTGAG
    CTTTCCCAAG AGGCCATGGA GGCATTGCTT GTTTTACATC AGCCAGAAAC CATTGAGCTA
    TGGAACCCGG ACGCTCCCAT TGAGACCTTC TGGGACATCA GCTCTCAGGT GTTATTTCTA
    ATTTGTAAGA AGCTGATTGG ACACCAGATG ATCAACAGTA CAGAGATACT CAAGTGGCTC
    AGGGAGATTC TCATATGCAG GAACAAGTTT CTATTAAAGA ACAAGGAGTG TGCTACACAG
    GGCAGTCTGA TCCCCATCAG CAGACAGGCT CAGACCAAGC TGGAGGTGTG TCTGTACACG
    TTCCTGTGGA GTCCTGATGC AGAGGCTGTT TTGGTGGCCA TGTCCTGTTT CAGGCATCTC
    TGTGAGGAGG CCGATATTCG CTGCAGTGTC GATGAGCTGC CTGTTCACAC AGTCCTACCC
    AATTACAACA CCTTCATTGA GCTTGCATCC GTCAGCAATA TGATGGCCAC AGGTCGCAAC
    ACACTACAAA AAAGAGTGAT GGCACTTTTA AGAAGAATAA AGCACCCTAC AGCTGGAAAT
    GTAGAGGCAT GGGAAGACAC ATATGCAAAG TGGGAGCAAG CTACAAAACA GATACTCAAC
    CACCCAAAAA ACAAAGTGGA AGATGGACAG GTAGTTAACA TAAGATTGGT CAATTATTTC
    ATCTTTTTTT CCATTCATGG TCATCTATTT TCTGTTCGTC CTCAGGAGTG GATCAACATG
    ACTGGTTTCT TGTGCGCTCT GGGAGGTGTT TGTCTCCAAC AGCGCAGCAC GCCGAGTCTG
    GGCACCTACA GTCCTCCAAT GGGCCCCCTG ACCGAACGCA AGGGGTCCAT GATTTCCATG
    AGCTCCTGCG AGGTAAACAG CGAAACATCC CTGAATCGCT TTCTGGAGCG TCTGCTGGCT
    CTGATGGTCT GCACACATGA GAAAGTGGGA GTGCAAATTC GCACCAACGT CAAAGACCTG
    GTGGGACTGG AGCTCAGTCC GGCTCTCTAT TCAATGCTCT TCACTAAGCT GCGCAACAGC
    ATCGGACGCT TCTTTGACAC CCAGGGGCCG GTTCCTATTA CAGATGCAAA CACTCTGTTT
    GTAGAGCAGA CCATTGCTAT CATGAAGAAC TTGCTGGACA GTCACACTGA AGGAAGCTCA
    GAGCACCTTG GTCAAGCCAG CATTGAGACC ATGATGCTCA ATCTAGTTAG GTATGTGCGT
    ATTTTAGGGA ACATTGTTCA TGCCATTCAG ATCAAAACCA AGTTGTGTCA GTTAGTGGAG
    GTGATGATGG AGCGCAGGGA TGACCTCTCC TTCTGCCAGG AGATGAAATT CAGGAATAAA
    ATGGTGGAGT ACCTCACAGA CTGGGTGATG GGAACCTCCA ATCAAGCTGC GGACGATGAT
    GTCAAATGCC TGACCCGCGA TCTGGACCAG GCCAGTATGG AGGCTGTCGT GTCTCTGTTG
    GCTGGTCTTC CTCTACAGCC TGAGGAGGGT GACGGTGTTG AGCTCATGGA GGCCAAATCA
    CAGCTCTTCC TGAAGTACTT CACACTGTTC ATGAACCTGT TGAATGACTG CAGCGAGGTT
    GAGGAAGATG GCCAGCAGGT CGCAGGGAGG AAACGTGGCA TGTCTAGACG ACTGGCATCT
    CTGCGGCACT GCACAGTATT GGCTATGTCT AACTTGCTCA ATGCTAATGT GGATAGCGGC
    CTCATGCACT CAATCGGTCT GGGATACCAT AAGGACCTGC AGACACGGGC TACATTTATG
    GAAGTGCTCA CTAAGATCCT TCAGCAAGGG ACGGAGTTCG ACACGCTTGC AGAGACTGTG
    CTTGCAGATC GCTTTGAGAG ACTGGTCGAG CTGGTCACCA TGATGGGAGA TCAGGGAGAG
    CTGCCCATAG CCATGGCTCT GGCCAATGTG GTCCCATGCT CTCAATGGGA TGAGCTGGCC
    AGAGTGTTAG TGACTCTCTT CGACTCTCGC CATCTGCTGT ATCAGCTGTT GTGGAATATG
    TTCTCCAAAG AGGTGGAGCT GGCAGACTCA ATGCAGACGC TCTTTAGAGG AAATAGCCTA
    GCCAGCAAGA TTATGACCTT CTGCTTCAAG GTGTACGGAG CTGCGTATCT ACAGAAGCTT
    CTGGAGCCGC TGTTGAGAGG AATCATCACT ACACCAGAGT GGCAGCAGAT CAGTTTTGAA
    GTGGACCCTA CTAGACTTGA ACAGGCAGAG AACCTGGAGG AGAACCAGAA GAACCTGCTT
    CAGATCACTG ACCGCTTCTT CCTGGCCATC ATCAACTCCT CGAGCGAGTT TCCTCCACAG
    CTGCGCAGTG TGTGCCACTG TCTGTATCAG GTGGTCAGTC AGCGGTTTCC TCAGAACAGC
    ATTGGTGCTG TAGGAAGTGC CATGTTCCTG CGCTTTATTA ACCCAGCTAT CGTGTCCCCA
    TATGAGGCTG GAATCCTAGA CAAGAAACCC CCACCTCGCA TCGAGCGTGG CCTTAAACTC
    ATGTCTAAGA TTCTGCAGAG CATTGCTAAT CATGTTCTCT TCACAAAAGA GGAGCATATG
    AGGCCCTTTA ATGATTTTGT CAAAAGCAAC TTCGATGCAG CCAGAAGGTT CTTCTTGGAT
    ATCGCATCTG ATTCTCCACC AAGTGATTCT GTGAACCATA GTCTGTCTTT CATCAGTGAC
    GGTAATGTGC TCGCTCTGCA CCGGCTATTA TGGAACAACC AGGAAAGAAT TGGCCAATAC
    CTGTCCAGCA ACAGGGACCA CAAGGCAGTG GGCCGTAGAC CATTTGACAA GATGGCCACT
    CTTCTGGCGT ACCTGGGTCC TCCTGAACAT AAGCCAGTAG CAGACACACA CTGGTCCAGC
    CTCAACCTCA CCAGCTCAAA GTTTGAGGAG TTCATGACAA GGCATCAAGT CCATGAGAAG
    GAGGAATTTA AAGCATTAAA AACCCTCAAC ATATTTTACC AAGCTGGCAC ATCTAAGAAC
    GGCAACCCTG TGTTTTATTA CATCGCACGC AGGTTCAAAA CTGGACAAAT CAATGGTGAT
    CTTCTGATCT ACCACGTCCT GCTGACGCTC AAGCCCTACT ATGCCAAGCA TTATGAGATC
    GTAGTTGACC TTACTCATGT GGGCCCCAGT AACCGTTTCA AGACGGACTT CTTATCCAAA
    TGGTTTGTGG TCTTTCCAAA CTTCGCCTAT GAGAACGTGG CTGCTGTGTA CATCTACAAC
    TGCAACACCT GGGTACGCGA GTACACCAAA TACCACGAGC GACTGCTGAC TGGCCTCAAG
    GGGAGCAAGA AGCTGGTGTT TATCGATTCG CCTGCACGTC TAGCTGAACA CATCGAACCC
    GAACAGCAGA AGCTGCCCGC AGCTACTTTA GCACTGGAAG AAGACCTCAA AGTGTTCCAC
    AACGCCCTAA AGCTTGCACA CAGAGACACA AAAGTGTCAA TTAAGGTGGG ATCCACAGCT
    GTACAGGTGA CCTCGGCCGA AAGAACGCGT GTCCTAGGAC AGCCTGTGTT CCTAAATGAC
    ATCTACTACG CTTCAGAGAT CGAGGAGATA TGCCTGGTGG ACGAGAACCA GTTCACGCTA
    ACCATCGCTA ACCAGGGCAC CCCACTCACT TTCATGCACC AGGAGTGTGA AGCCATCGTT
    CAGTCCATCA TCCACATCCG CACGCGCTGG GAACTTTCCC AACCAGACTC CATTCCCCAA
    CACACCAAAA TCAGGCCTAA GGATGTTCCT GGCACCCTGC TCAACATCGC TCTTCTCAAC
    CTGGGAAGCT CCGACCCCAG TCTGAGGTCT GCGGCCTACA ATCTCCTTTG TGCCTTAACC
    TGCACCTTCA ACCTGAAGAT TGAAGGCCAG CTTCTGGAAA CGTCTGGCTT GTGCATCCCA
    GCCAACAACA CGCTTTTTAT TGTGTCCATT AGCAAAACGC TAGCAGCTAA TGAACCCCAC
    CTCACTCTGG AGTTCCTGGA AGAGTGCATC TCAGGATTCA GTAAATCAAG TATAGAGCTG
    AAGCATCTGT GTCTAGAATA TATGACTCCT TGGCTCCGTA ATTTGGTGCG CTTTTGCAAA
    CACAATGATG ACGCCAAACG GCAGCGTGTC ACGGCCATCC TGGACAAGCT TATTACTATG
    ACCATCAACG AGAAGCAGAT GTACCCCTCC ATACAGGCCA AGATATGGGG CAGCTTGGGT
    CAGATCACAG ACCTGCTTGA TGTGGTGTTG GACAGCTTTA TAAAGACCAG CGCTACTGGC
    GGTTTAGGAT CCATCAAAGC TGAAGTGATG GCTGACACTG CTGTAGCTTT GGCGTCAGGA
    AATGTCAAAC TGGTCTCCAG CAAGGTGATT GGCCGAATGT GTAAGATCAT CGACAAGACG
    TGTCTGTCTC CCACTCCGAC CCTTGAGCAG CACCTCATGT GGGACGACAT CGCCATCCTG
    GCCCGGTACA TGCTCATGCT GTCCTTCAAC AACTCTCTGG ACGTGGCCGC TCATCTGCCA
    TACCTGTTCC ACGTCGTCAC CCTGCTGGTG GCCACGGGCC CTCTCTCGCT GCGTGCATCA
    ACACACGGCC TGGTCATCAA CATCATTCAC TCGCTCTGCA CATGCTCACA ACTCAACTTC
    AGTGAGGAGA CTAAACAGGT GCTGCGCCTG AGTCTGACTG AGTTCTCCCT GCCCAAGTTC
    TACCTGCTGT TCGGCATCAG TAAGGTCAAA TCTGCGGCGG TCATCGCTTT CCGTTCCAGC
    TACAGAGACC GCTCATTCTC TCCAGGCTCT TATGAGAGAG AGACATTTGC TCTGTCTTCC
    CTGGAAACAG TCACGGAGGC TCTTTTGGAG ATCATGGAGG CCTGCATGAG GGACATTCCT
    GGATGCAAAT GGCTAGATCA GTGGACTGAA TTGGCGCAAA AGTTTGCGTT CCAGTATAAC
    CCCTCTCTTC AGCCTCGGGC GCTGGTGGTG TTCGGCTGCA TCAGTAAACG AGTGACTCAC
    GGACAGCTAA AGCAGATCAT TCGTATTCTC AGTAAAGCCA GTCCTCTACT CAGCATTGAT
    CGGGGGCTGG AGAGCTGCTT AAAGGGCCCA GATAATTACA ACAGCCAGGT TCTCATCGAA
    GCCACAGTCA TCGCTCTCAC CAAACTACAG CCTCTCCTCG ATCAGGATTC TCCTATGCAT
    AAAGCTCTGT TCTGGGTGGC CGTGGCCGTG CTTCAGTTGG ATGAAGTGAA TCTGTACTCC
    GCCGGCACTG CCTTACTGGA GCAGAACCTG CACACACTGG ACAGCCTCCG AGTGTTTAAT
    GATAAGAGTC CTGAAGAGGT TTTTATGGAG ATCCGGAAAC CTCTAGAGTG GCACTGTAAA
    CAAATGGACC ATTTTGTGGG ACTCAACTTT GCCTCCAACT TCAATTTTGC ATTAGTGGGC
    CATTTACTTA AAGGATACAG ACATCCCTCT TCAACAACAG TTGCCAGGAC AGTTCGTATC
    CTGCATACAC TCCTGGGCCT GGTGGGAAAA CACCGCAGCT GTGACAAGTT TGAGGTGAAC
    ACGCAGAGTG TGGCCTATCT GGCAGCTTTG CTCACAGTGT CAGAGGAAGT TAGGAGCCGC
    TGCAGTTTAA AGCACAGGAA ATCATTGCTG CTTTCAGAAG TTACAATGGA GAACTTCCCC
    ATGGACACAT ACACCGTTTA CGACACTGAA CCCGGGTGCA GGCTACTGCG TGAAAACCAG
    CCTTGGGCTT CTCCGAAAGT GTCAGAGCGC AGTCTTGCAC CTCCGTACTC CACAATAGGG
    CAGACCAGCC TTCGTCCACG CAAATCCATG AGTGTAGACA TGGGCCAGCC GTCTCAGGCC
    AATGCGAAGA AACTACTGGG TATTAGCGGC ACTAGAAAGA GTTTCGATCA CTTAATATCA
    GAATCCAAAG CCCCAAAAAG ACCAGACATG GAGTCGGGCA TCACCACACC TCCAAAGATG
    AGGCGGGTTT CTGAAAATGA CTATGAGATC GAAACTCAGC GAATAGCCAA CTCTCATTTA
    CGCAAAGTGT CTGTGTCTGA ATCCAATGTC CTCCTGGACG AGAACGTACT TACGGACCCC
    AAGATCCAGA CGTTGCTCCT CACTGTACTG GCAACACTGG TGAAGTACAC TACAGACGAG
    TTTCATCAGC GGATCTTGTA TGAGTATTTG GCTGAGGCCA GTGTGGTCTT CCCCAAGGTC
    TTTCCAGTTG TGCACAACCT GCTGGACTCC AAGTTAAGCA CCGTCCTTTC CATGTGCCAG
    GACCCGAACC TTCTGAACCC CGTCCATGGC ATCATTCAGA ACGTGGTCTA TCATGAGGAG
    TCTCCCCCTC AGTACCAGCC GTCCTGCTTG CAGAGCTTTG GTTTCAATGG TCTTTGGAGG
    TTTGCCGGGC CGTTCTCTAA GCAAACCCAA ATTCCAGACT ACGCCGAGCT GGTGGTTAAA
    TTTTTGGATG CTTTGATTGA CACGTATCTC CCGGGCATTG AGGAGGAAGG AGGAGAAGAG
    TCACTGCGGA CCCCGACCTC GCCATATCCA CCAACCGTCC AGAGCCAGCT GAGCATCACA
    GCCAATCTCA ACCTGTCCAA CTCCATGACA TCACTTGCCA CATCACAGCA CTCCCCAGAG
    GGCCCTATAG TTTATAGACG GTCCCCAACT CTCTCCCATC CACGAACTAC AGAGGAGGTG
    CCACCGAAAA CAGCTGCCTG TCTGCCTTGC TCTAAGTCGG CTGTATTCCC ACCTCACCTC
    GTCCTGCCAG GTACTCATCC ACCTCTGCTG TGTGAAAGTA GCAGTTGTTG GCATTGGCCC
    GAGTGTTTGA ATTGTAAATC TGCTGCTGTC AGATCAAAGA AAGCAAGAGG GGCTACTATA
    ACAGATAGTG TGAAGAGAGA GCACAAGGGC CTGGTATTAA GCAAACACTT ACATACAGTA
    CATAGCCAGG GGACAGGACA TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017206680.1
    CDS253..8736
    Translation

    Target ORF information:

    RefSeq Version XM_017351191.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio neurofibromin 1a (nf1a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351191.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCGGCGC ACAAGCCCGT GGAATGGGTC CAGGCCATCA TTAACAGATT CGACGAACAG 
    CTCCCAATCA AAACAGGACA GCAGAGCACC CACACCAAAG TGAGTACAGA GCACAACAAA
    GAATGTTTGA TCAACATCTC CAAGTACAAA TTTGCCCTCG TCATCAATGG CCTGACCAAC
    ATACTCAAAA ATGTGAACAA CATGCGGATA TTTGGCGAAG CTGCTGAGAA GAACCTCTAC
    TTCTCCCAGC TGATCATACT GGACACACTG GAGAAGTGTC TAGCAGGGCA ACCTAAGGAT
    TGCCTGAGGC TGGATGAGGC TATGTTGGTA AAACAGTTGC TGCCTGAAAT CTGTCACTTC
    CTGCACTCGT ACCGTGAGGG CCAGCAACAC GCCACTCAGC TGCGCAGCTC CGCCTCTGCT
    GTGCTTTTCT CTCTCAGCTG CAACAACTTT AATGCCGTCT TCAGTCGTAT CTCCACCAGG
    TTGCAAGAGC TCACAGTTTG TACAGAGGAC ACAGTGGATG TCCATGACAT TGAGCTCATT
    CAGTATATCA ATGTAGATTG CTCCAAGTTA AAGAGACTCC TACAAGAAGC AGTACTTAAG
    TTCAAGTCTT TGAAAAAGCC GGCTCAGCTT GCAGTAATCA GCAGTTTGGA AAAGGCTTTC
    TGGAACTGGG TTGAACACTA CCCTGATGAT TTCACTATGC TCTATCAGAG ACCCCAGGCC
    GACATGGCCG ACTGTGCAGA GAGACTGTTC GACCTAGTGG ACAGTTTTGC GGAAAGCGCA
    AAGAGAAAAG CTGCTGTGTG GCCACTGCAG ATCATCCTCC TCATCCTCTG TCCAGAGATC
    ACGCAGAGCA TCACCAAAGA AGTAGGGGGA GAGGAGAAAG CTAACAAGAA GTCATTTGTA
    GACAGTTTAC GAAAGGCTCT TGGCCAACAC AGCGGCAGCA AACAATTGAC GGAAAGTGCA
    GCTGTTGCAT GCGTCAAACT CTGCAAAGCC TGCACATATA TCAACTGGGA GGATAATTCT
    GTCATCTTTC TCCTGGTTCA GTCCATCGTC ATGGACCTTA AGGGTCTCCT GTTTAACCCC
    ACCAAACCTT TCTCAAGAGG TACGGGCTGC CAGAGCGCCG ATGTAGATTT GATGATCGAC
    TGCTTTGTGT CATGTTATCG CATCAATCCT CGCAACAACC AGCATTTTAA GGTGTGTCTG
    GCCTCGACTT CTCCTCCTAC CTTCCATTTC GTCCTTGTCA ACTCCCTGCA CAAAATTATC
    ACAAATTCTC ACCTGGACTG GTGGCCGAAG ATTGACGCCA TCTACTGCTA CTCAGGAGAA
    CTACGCACCA TGTTTGCAGA CACACTGGGT CGAGTGATTC AGAGTCTCAG TGCACATCCG
    CCTCTGAGGA TGACATCAAG TTTGACATTC AAAGAGAAAA TGACAACAAG TTTGAAGTTC
    AAGGAAAAGT CCACAGATCT TGATTCGCGT TCTTTGCTGC TGGCACTGGT CAAACTTATC
    CACGCTGACC CCAAACTAAT GCTGCACAAT CCGGGAAAAC CTGGTCAGGA GATTCAGAAC
    AGCACAGCTG AGATCATCAA AGGTCTGGTT CAGCTGGTTC CTCTTACCCA CAGTGCTGAG
    CTTTCCCAAG AGGCCATGGA GGCATTGCTT GTTTTACATC AGCCAGAAAC CATTGAGCTA
    TGGAACCCGG ACGCTCCCAT TGAGACCTTC TGGGACATCA GCTCTCAGGT GTTATTTCTA
    ATTTGTAAGA AGCTGATTGG ACACCAGATG ATCAACAGTA CAGAGATACT CAAGTGGCTC
    AGGGAGATTC TCATATGCAG GAACAAGTTT CTATTAAAGA ACAAGGAGTG TGCTACACAG
    GGCAGTCTGA TCCCCATCAG CAGACAGGCT CAGACCAAGC TGGAGGTGTG TCTGTACACG
    TTCCTGTGGA GTCCTGATGC AGAGGCTGTT TTGGTGGCCA TGTCCTGTTT CAGGCATCTC
    TGTGAGGAGG CCGATATTCG CTGCAGTGTC GATGAGCTGC CTGTTCACAC AGTCCTACCC
    AATTACAACA CCTTCATTGA GCTTGCATCC GTCAGCAATA TGATGGCCAC AGGTCGCAAC
    ACACTACAAA AAAGAGTGAT GGCACTTTTA AGAAGAATAA AGCACCCTAC AGCTGGAAAT
    GTAGAGGCAT GGGAAGACAC ATATGCAAAG TGGGAGCAAG CTACAAAACA GATACTCAAC
    CACCCAAAAA ACAAAGTGGA AGATGGACAG GTAGTTAACA TAAGATTGGT CAATTATTTC
    ATCTTTTTTT CCATTCATGG TCATCTATTT TCTGTTCGTC CTCAGGAGTG GATCAACATG
    ACTGGTTTCT TGTGCGCTCT GGGAGGTGTT TGTCTCCAAC AGCGCAGCAC GCCGAGTCTG
    GGCACCTACA GTCCTCCAAT GGGCCCCCTG ACCGAACGCA AGGGGTCCAT GATTTCCATG
    AGCTCCTGCG AGGTAAACAG CGAAACATCC CTGAATCGCT TTCTGGAGCG TCTGCTGGCT
    CTGATGGTCT GCACACATGA GAAAGTGGGA GTGCAAATTC GCACCAACGT CAAAGACCTG
    GTGGGACTGG AGCTCAGTCC GGCTCTCTAT TCAATGCTCT TCACTAAGCT GCGCAACAGC
    ATCGGACGCT TCTTTGACAC CCAGGGGCCG GTTCCTATTA CAGATGCAAA CACTCTGTTT
    GTAGAGCAGA CCATTGCTAT CATGAAGAAC TTGCTGGACA GTCACACTGA AGGAAGCTCA
    GAGCACCTTG GTCAAGCCAG CATTGAGACC ATGATGCTCA ATCTAGTTAG GTATGTGCGT
    ATTTTAGGGA ACATTGTTCA TGCCATTCAG ATCAAAACCA AGTTGTGTCA GTTAGTGGAG
    GTGATGATGG AGCGCAGGGA TGACCTCTCC TTCTGCCAGG AGATGAAATT CAGGAATAAA
    ATGGTGGAGT ACCTCACAGA CTGGGTGATG GGAACCTCCA ATCAAGCTGC GGACGATGAT
    GTCAAATGCC TGACCCGCGA TCTGGACCAG GCCAGTATGG AGGCTGTCGT GTCTCTGTTG
    GCTGGTCTTC CTCTACAGCC TGAGGAGGGT GACGGTGTTG AGCTCATGGA GGCCAAATCA
    CAGCTCTTCC TGAAGTACTT CACACTGTTC ATGAACCTGT TGAATGACTG CAGCGAGGTT
    GAGGAAGATG GCCAGCAGGT CGCAGGGAGG AAACGTGGCA TGTCTAGACG ACTGGCATCT
    CTGCGGCACT GCACAGTATT GGCTATGTCT AACTTGCTCA ATGCTAATGT GGATAGCGGC
    CTCATGCACT CAATCGGTCT GGGATACCAT AAGGACCTGC AGACACGGGC TACATTTATG
    GAAGTGCTCA CTAAGATCCT TCAGCAAGGG ACGGAGTTCG ACACGCTTGC AGAGACTGTG
    CTTGCAGATC GCTTTGAGAG ACTGGTCGAG CTGGTCACCA TGATGGGAGA TCAGGGAGAG
    CTGCCCATAG CCATGGCTCT GGCCAATGTG GTCCCATGCT CTCAATGGGA TGAGCTGGCC
    AGAGTGTTAG TGACTCTCTT CGACTCTCGC CATCTGCTGT ATCAGCTGTT GTGGAATATG
    TTCTCCAAAG AGGTGGAGCT GGCAGACTCA ATGCAGACGC TCTTTAGAGG AAATAGCCTA
    GCCAGCAAGA TTATGACCTT CTGCTTCAAG GTGTACGGAG CTGCGTATCT ACAGAAGCTT
    CTGGAGCCGC TGTTGAGAGG AATCATCACT ACACCAGAGT GGCAGCAGAT CAGTTTTGAA
    GTGGACCCTA CTAGACTTGA ACAGGCAGAG AACCTGGAGG AGAACCAGAA GAACCTGCTT
    CAGATCACTG ACCGCTTCTT CCTGGCCATC ATCAACTCCT CGAGCGAGTT TCCTCCACAG
    CTGCGCAGTG TGTGCCACTG TCTGTATCAG GTGGTCAGTC AGCGGTTTCC TCAGAACAGC
    ATTGGTGCTG TAGGAAGTGC CATGTTCCTG CGCTTTATTA ACCCAGCTAT CGTGTCCCCA
    TATGAGGCTG GAATCCTAGA CAAGAAACCC CCACCTCGCA TCGAGCGTGG CCTTAAACTC
    ATGTCTAAGA TTCTGCAGAG CATTGCTAAT CATGTTCTCT TCACAAAAGA GGAGCATATG
    AGGCCCTTTA ATGATTTTGT CAAAAGCAAC TTCGATGCAG CCAGAAGGTT CTTCTTGGAT
    ATCGCATCTG ATTCTCCACC AAGTGATTCT GTGAACCATA GTCTGTCTTT CATCAGTGAC
    GGTAATGTGC TCGCTCTGCA CCGGCTATTA TGGAACAACC AGGAAAGAAT TGGCCAATAC
    CTGTCCAGCA ACAGGGACCA CAAGGCAGTG GGCCGTAGAC CATTTGACAA GATGGCCACT
    CTTCTGGCGT ACCTGGGTCC TCCTGAACAT AAGCCAGTAG CAGACACACA CTGGTCCAGC
    CTCAACCTCA CCAGCTCAAA GTTTGAGGAG TTCATGACAA GGCATCAAGT CCATGAGAAG
    GAGGAATTTA AAGCATTAAA AACCCTCAAC ATATTTTACC AAGCTGGCAC ATCTAAGAAC
    GGCAACCCTG TGTTTTATTA CATCGCACGC AGGTTCAAAA CTGGACAAAT CAATGGTGAT
    CTTCTGATCT ACCACGTCCT GCTGACGCTC AAGCCCTACT ATGCCAAGCA TTATGAGATC
    GTAGTTGACC TTACTCATGT GGGCCCCAGT AACCGTTTCA AGACGGACTT CTTATCCAAA
    TGGTTTGTGG TCTTTCCAAA CTTCGCCTAT GAGAACGTGG CTGCTGTGTA CATCTACAAC
    TGCAACACCT GGGTACGCGA GTACACCAAA TACCACGAGC GACTGCTGAC TGGCCTCAAG
    GGGAGCAAGA AGCTGGTGTT TATCGATTCG CCTGCACGTC TAGCTGAACA CATCGAACCC
    GAACAGCAGA AGCTGCCCGC AGCTACTTTA GCACTGGAAG AAGACCTCAA AGTGTTCCAC
    AACGCCCTAA AGCTTGCACA CAGAGACACA AAAGTGTCAA TTAAGGTGGG ATCCACAGCT
    GTACAGGTGA CCTCGGCCGA AAGAACGCGT GTCCTAGGAC AGCCTGTGTT CCTAAATGAC
    ATCTACTACG CTTCAGAGAT CGAGGAGATA TGCCTGGTGG ACGAGAACCA GTTCACGCTA
    ACCATCGCTA ACCAGGGCAC CCCACTCACT TTCATGCACC AGGAGTGTGA AGCCATCGTT
    CAGTCCATCA TCCACATCCG CACGCGCTGG GAACTTTCCC AACCAGACTC CATTCCCCAA
    CACACCAAAA TCAGGCCTAA GGATGTTCCT GGCACCCTGC TCAACATCGC TCTTCTCAAC
    CTGGGAAGCT CCGACCCCAG TCTGAGGTCT GCGGCCTACA ATCTCCTTTG TGCCTTAACC
    TGCACCTTCA ACCTGAAGAT TGAAGGCCAG CTTCTGGAAA CGTCTGGCTT GTGCATCCCA
    GCCAACAACA CGCTTTTTAT TGTGTCCATT AGCAAAACGC TAGCAGCTAA TGAACCCCAC
    CTCACTCTGG AGTTCCTGGA AGAGTGCATC TCAGGATTCA GTAAATCAAG TATAGAGCTG
    AAGCATCTGT GTCTAGAATA TATGACTCCT TGGCTCCGTA ATTTGGTGCG CTTTTGCAAA
    CACAATGATG ACGCCAAACG GCAGCGTGTC ACGGCCATCC TGGACAAGCT TATTACTATG
    ACCATCAACG AGAAGCAGAT GTACCCCTCC ATACAGGCCA AGATATGGGG CAGCTTGGGT
    CAGATCACAG ACCTGCTTGA TGTGGTGTTG GACAGCTTTA TAAAGACCAG CGCTACTGGC
    GGTTTAGGAT CCATCAAAGC TGAAGTGATG GCTGACACTG CTGTAGCTTT GGCGTCAGGA
    AATGTCAAAC TGGTCTCCAG CAAGGTGATT GGCCGAATGT GTAAGATCAT CGACAAGACG
    TGTCTGTCTC CCACTCCGAC CCTTGAGCAG CACCTCATGT GGGACGACAT CGCCATCCTG
    GCCCGGTACA TGCTCATGCT GTCCTTCAAC AACTCTCTGG ACGTGGCCGC TCATCTGCCA
    TACCTGTTCC ACGTCGTCAC CCTGCTGGTG GCCACGGGCC CTCTCTCGCT GCGTGCATCA
    ACACACGGCC TGGTCATCAA CATCATTCAC TCGCTCTGCA CATGCTCACA ACTCAACTTC
    AGTGAGGAGA CTAAACAGGT GCTGCGCCTG AGTCTGACTG AGTTCTCCCT GCCCAAGTTC
    TACCTGCTGT TCGGCATCAG TAAGGTCAAA TCTGCGGCGG TCATCGCTTT CCGTTCCAGC
    TACAGAGACC GCTCATTCTC TCCAGGCTCT TATGAGAGAG AGACATTTGC TCTGTCTTCC
    CTGGAAACAG TCACGGAGGC TCTTTTGGAG ATCATGGAGG CCTGCATGAG GGACATTCCT
    GGATGCAAAT GGCTAGATCA GTGGACTGAA TTGGCGCAAA AGTTTGCGTT CCAGTATAAC
    CCCTCTCTTC AGCCTCGGGC GCTGGTGGTG TTCGGCTGCA TCAGTAAACG AGTGACTCAC
    GGACAGCTAA AGCAGATCAT TCGTATTCTC AGTAAAGCCA GTCCTCTACT CAGCATTGAT
    CGGGGGCTGG AGAGCTGCTT AAAGGGCCCA GATAATTACA ACAGCCAGGT TCTCATCGAA
    GCCACAGTCA TCGCTCTCAC CAAACTACAG CCTCTCCTCG ATCAGGATTC TCCTATGCAT
    AAAGCTCTGT TCTGGGTGGC CGTGGCCGTG CTTCAGTTGG ATGAAGTGAA TCTGTACTCC
    GCCGGCACTG CCTTACTGGA GCAGAACCTG CACACACTGG ACAGCCTCCG AGTGTTTAAT
    GATAAGAGTC CTGAAGAGGT TTTTATGGAG ATCCGGAAAC CTCTAGAGTG GCACTGTAAA
    CAAATGGACC ATTTTGTGGG ACTCAACTTT GCCTCCAACT TCAATTTTGC ATTAGTGGGC
    CATTTACTTA AAGGATACAG ACATCCCTCT TCAACAACAG TTGCCAGGAC AGTTCGTATC
    CTGCATACAC TCCTGGGCCT GGTGGGAAAA CACCGCAGCT GTGACAAGTT TGAGGTGAAC
    ACGCAGAGTG TGGCCTATCT GGCAGCTTTG CTCACAGTGT CAGAGGAAGT TAGGAGCCGC
    TGCAGTTTAA AGCACAGGAA ATCATTGCTG CTTTCAGAAG TTACAATGGA GAACTTCCCC
    ATGGACACAT ACACCGTTTA CGACACTGAA CCCGGGTGCA GGCTACTGCG TGAAAACCAG
    CCTTGGGCTT CTCCGAAAGT GTCAGAGCGC AGTCTTGCAC CTCCGTACTC CACAATAGGG
    CAGACCAGCC TTCGTCCACG CAAATCCATG AGTGTAGACA TGGGCCAGCC GTCTCAGGCC
    AATGCGAAGA AACTACTGGG TATTAGCGGC ACTAGAAAGA GTTTCGATCA CTTAATATCA
    GAATCCAAAG CCCCAAAAAG ACCAGACATG GAGTCGGGCA TCACCACACC TCCAAAGATG
    AGGCGGGTTT CTGAAAATGA CTATGAGATC GAAACTCAGC GAATAGCCAA CTCTCATTTA
    CGCAAAGTGT CTGTGTCTGA ATCCAATGTC CTCCTGGACG AGAACGTACT TACGGACCCC
    AAGATCCAGA CGTTGCTCCT CACTGTACTG GCAACACTGG TGAAGTACAC TACAGACGAG
    TTTCATCAGC GGATCTTGTA TGAGTATTTG GCTGAGGCCA GTGTGGTCTT CCCCAAGGTC
    TTTCCAGTTG TGCACAACCT GCTGGACTCC AAGTTAAGCA CCGTCCTTTC CATGTGCCAG
    GACCCGAACC TTCTGAACCC CGTCCATGGC ATCATTCAGA ACGTGGTCTA TCATGAGGAG
    TCTCCCCCTC AGTACCAGCC GTCCTGCTTG CAGAGCTTTG GTTTCAATGG TCTTTGGAGG
    TTTGCCGGGC CGTTCTCTAA GCAAACCCAA ATTCCAGACT ACGCCGAGCT GGTGGTTAAA
    TTTTTGGATG CTTTGATTGA CACGTATCTC CCGGGCATTG AGGAGGAAGG AGGAGAAGAG
    TCACTGCGGA CCCCGACCTC GCCATATCCA CCAACCGTCC AGAGCCAGCT GAGCATCACA
    GCCAATCTCA ACCTGTCCAA CTCCATGACA TCACTTGCCA CATCACAGCA CTCCCCAGAG
    GGCCCTATAG TTTATAGACG GTCCCCAACT CTCTCCCATC CACGAACTAC AGAGGAGGTG
    CCACCGAAAA CAGCTGCCTG TCTGCCTTGC TCTAAGTCGG CTGTATTCCC ACCTCACCTC
    GTCCTGCCAG GTACTCATCC ACCTCTGCTG TGTGAAAGTA GCAGTTGTTG GCATTGGCCC
    GAGTGTTTGA ATTGTAAATC TGCTGCTGTC AGATCAAAGA AAGCAAGAGG GGCTACTATA
    ACAGATAGTG TGAAGAGAGA GCACAAGGGC CTGGTATTAA GCAAACACTT ACATACAGTA
    CATAGCCAGG GGACAGGACA TTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55269
    Clone ID Related Accession (Same CDS sequence) XM_017351192.1
    Accession Version XM_017351192.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8475bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product neurofibromin isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCGGCGC ACAAGCCCGT GGAATGGGTC CAGGCCATCA TTAACAGATT CGACGAACAG 
    CTCCCAATCA AAACAGGACA GCAGAGCACC CACACCAAAG TGAGTACAGA GCACAACAAA
    GAATGTTTGA TCAACATCTC CAAGTACAAA TTTGCCCTCG TCATCAATGG CCTGACCAAC
    ATACTCAAAA ATGTGAACAA CATGCGGATA TTTGGCGAAG CTGCTGAGAA GAACCTCTAC
    TTCTCCCAGC TGATCATACT GGACACACTG GAGAAGTGTC TAGCAGGGCA ACCTAAGGAT
    TGCCTGAGGC TGGATGAGGC TATGTTGGTA AAACAGTTGC TGCCTGAAAT CTGTCACTTC
    CTGCACTCGT ACCGTGAGGG CCAGCAACAC GCCACTCAGC TGCGCAGCTC CGCCTCTGCT
    GTGCTTTTCT CTCTCAGCTG CAACAACTTT AATGCCGTCT TCAGTCGTAT CTCCACCAGG
    TTGCAAGAGC TCACAGTTTG TACAGAGGAC ACAGTGGATG TCCATGACAT TGAGCTCATT
    CAGTATATCA ATGTAGATTG CTCCAAGTTA AAGAGACTCC TACAAGAAGC AGTACTTAAG
    TTCAAGTCTT TGAAAAAGCC GGCTCAGCTT GCAGTAATCA GCAGTTTGGA AAAGGCTTTC
    TGGAACTGGG TTGAACACTA CCCTGATGAT TTCACTATGC TCTATCAGAG ACCCCAGGCC
    GACATGGCCG ACTGTGCAGA GAGACTGTTC GACCTAGTGG ACAGTTTTGC GGAAAGCGCA
    AAGAGAAAAG CTGCTGTGTG GCCACTGCAG ATCATCCTCC TCATCCTCTG TCCAGAGATC
    ACGCAGAGCA TCACCAAAGA AGTAGGGGGA GAGGAGAAAG CTAACAAGAA GTCATTTGTA
    GACAGTTTAC GAAAGGCTCT TGGCCAACAC AGCGGCAGCA AACAATTGAC GGAAAGTGCA
    GCTGTTGCAT GCGTCAAACT CTGCAAAGCC TGCACATATA TCAACTGGGA GGATAATTCT
    GTCATCTTTC TCCTGGTTCA GTCCATCGTC ATGGACCTTA AGGGTCTCCT GTTTAACCCC
    ACCAAACCTT TCTCAAGAGG TACGGGCTGC CAGAGCGCCG ATGTAGATTT GATGATCGAC
    TGCTTTGTGT CATGTTATCG CATCAATCCT CGCAACAACC AGCATTTTAA GGTGTGTCTG
    GCCTCGACTT CTCCTCCTAC CTTCCATTTC GTCCTTGTCA ACTCCCTGCA CAAAATTATC
    ACAAATTCTC ACCTGGACTG GTGGCCGAAG ATTGACGCCA TCTACTGCTA CTCAGGAGAA
    CTACGCACCA TGTTTGCAGA CACACTGGGT CGAGTGATTC AGAGTCTCAG TGCACATCCG
    CCTCTGAGGA TGACATCAAG TTTGACATTC AAAGAGAAAA TGACAACAAG TTTGAAGTTC
    AAGGAAAAGT CCACAGATCT TGATTCGCGT TCTTTGCTGC TGGCACTGGT CAAACTTATC
    CACGCTGACC CCAAACTAAT GCTGCACAAT CCGGGAAAAC CTGGTCAGGA GATTCAGAAC
    AGCACAGCTG AGATCATCAA AGGTCTGGTT CAGCTGGTTC CTCTTACCCA CAGTGCTGAG
    CTTTCCCAAG AGGCCATGGA GGCATTGCTT GTTTTACATC AGCCAGAAAC CATTGAGCTA
    TGGAACCCGG ACGCTCCCAT TGAGACCTTC TGGGACATCA GCTCTCAGGT GTTATTTCTA
    ATTTGTAAGA AGCTGATTGG ACACCAGATG ATCAACAGTA CAGAGATACT CAAGTGGCTC
    AGGGAGATTC TCATATGCAG GAACAAGTTT CTATTAAAGA ACAAGGAGTG TGCTACACAG
    GGCAGTCTGA TCCCCATCAG CAGACAGGCT CAGACCAAGC TGGAGGTGTG TCTGTACACG
    TTCCTGTGGA GTCCTGATGC AGAGGCTGTT TTGGTGGCCA TGTCCTGTTT CAGGCATCTC
    TGTGAGGAGG CCGATATTCG CTGCAGTGTC GATGAGCTGC CTGTTCACAC AGTCCTACCC
    AATTACAACA CCTTCATTGA GCTTGCATCC GTCAGCAATA TGATGGCCAC AGGTCGCAAC
    ACACTACAAA AAAGAGTGAT GGCACTTTTA AGAAGAATAA AGCACCCTAC AGCTGGAAAT
    GTAGAGGCAT GGGAAGACAC ATATGCAAAG TGGGAGCAAG CTACAAAACA GATACTCAAC
    CACCCAAAAA ACAAAGTGGA AGATGGACAG GTAGTTAACA TAAGATTGGT CAATTATTTC
    ATCTTTTTTT CCATTCATGG TCATCTATTT TCTGTTCGTC CTCAGGAGTG GATCAACATG
    ACTGGTTTCT TGTGCGCTCT GGGAGGTGTT TGTCTCCAAC AGCGCAGCAC GCCGAGTCTG
    GGCACCTACA GTCCTCCAAT GGGCCCCCTG ACCGAACGCA AGGGGTCCAT GATTTCCATG
    AGCTCCTGCG AGGTAAACAG CGAAACATCC CTGAATCGCT TTCTGGAGCG TCTGCTGGCT
    CTGATGGTCT GCACACATGA GAAAGTGGGA GTGCAAATTC GCACCAACGT CAAAGACCTG
    GTGGGACTGG AGCTCAGTCC GGCTCTCTAT TCAATGCTCT TCACTAAGCT GCGCAACAGC
    ATCGGACGCT TCTTTGACAC CCAGGGGCCG GTTCCTATTA CAGATGCAAA CACTCTGTTT
    GTAGAGCAGA CCATTGCTAT CATGAAGAAC TTGCTGGACA GTCACACTGA AGGAAGCTCA
    GAGCACCTTG GTCAAGCCAG CATTGAGACC ATGATGCTCA ATCTAGTTAG GTATGTGCGT
    ATTTTAGGGA ACATTGTTCA TGCCATTCAG ATCAAAACCA AGTTGTGTCA GTTAGTGGAG
    GTGATGATGG AGCGCAGGGA TGACCTCTCC TTCTGCCAGG AGATGAAATT CAGGAATAAA
    ATGGTGGAGT ACCTCACAGA CTGGGTGATG GGAACCTCCA ATCAAGCTGC GGACGATGAT
    GTCAAATGCC TGACCCGCGA TCTGGACCAG GCCAGTATGG AGGCTGTCGT GTCTCTGTTG
    GCTGGTCTTC CTCTACAGCC TGAGGAGGGT GACGGTGTTG AGCTCATGGA GGCCAAATCA
    CAGCTCTTCC TGAAGTACTT CACACTGTTC ATGAACCTGT TGAATGACTG CAGCGAGGTT
    GAGGAAGATG GCCAGCAGGT CGCAGGGAGG AAACGTGGCA TGTCTAGACG ACTGGCATCT
    CTGCGGCACT GCACAGTATT GGCTATGTCT AACTTGCTCA ATGCTAATGT GGATAGCGGC
    CTCATGCACT CAATCGGTCT GGGATACCAT AAGGACCTGC AGACACGGGC TACATTTATG
    GAAGTGCTCA CTAAGATCCT TCAGCAAGGG ACGGAGTTCG ACACGCTTGC AGAGACTGTG
    CTTGCAGATC GCTTTGAGAG ACTGGTCGAG CTGGTCACCA TGATGGGAGA TCAGGGAGAG
    CTGCCCATAG CCATGGCTCT GGCCAATGTG GTCCCATGCT CTCAATGGGA TGAGCTGGCC
    AGAGTGTTAG TGACTCTCTT CGACTCTCGC CATCTGCTGT ATCAGCTGTT GTGGAATATG
    TTCTCCAAAG AGGTGGAGCT GGCAGACTCA ATGCAGACGC TCTTTAGAGG AAATAGCCTA
    GCCAGCAAGA TTATGACCTT CTGCTTCAAG GTGTACGGAG CTGCGTATCT ACAGAAGCTT
    CTGGAGCCGC TGTTGAGAGG AATCATCACT ACACCAGAGT GGCAGCAGAT CAGTTTTGAA
    GTGGACCCTA CTAGACTTGA ACAGGCAGAG AACCTGGAGG AGAACCAGAA GAACCTGCTT
    CAGATCACTG ACCGCTTCTT CCTGGCCATC ATCAACTCCT CGAGCGAGTT TCCTCCACAG
    CTGCGCAGTG TGTGCCACTG TCTGTATCAG GTGGTCAGTC AGCGGTTTCC TCAGAACAGC
    ATTGGTGCTG TAGGAAGTGC CATGTTCCTG CGCTTTATTA ACCCAGCTAT CGTGTCCCCA
    TATGAGGCTG GAATCCTAGA CAAGAAACCC CCACCTCGCA TCGAGCGTGG CCTTAAACTC
    ATGTCTAAGA TTCTGCAGAG CATTGCTAAT CATGTTCTCT TCACAAAAGA GGAGCATATG
    AGGCCCTTTA ATGATTTTGT CAAAAGCAAC TTCGATGCAG CCAGAAGGTT CTTCTTGGAT
    ATCGCATCTG ATTCTCCACC AAGTGATTCT GTGAACCATA GTCTGTCTTT CATCAGTGAC
    GGTAATGTGC TCGCTCTGCA CCGGCTATTA TGGAACAACC AGGAAAGAAT TGGCCAATAC
    CTGTCCAGCA ACAGGGACCA CAAGGCAGTG GGCCGTAGAC CATTTGACAA GATGGCCACT
    CTTCTGGCGT ACCTGGGTCC TCCTGAACAT AAGCCAGTAG CAGACACACA CTGGTCCAGC
    CTCAACCTCA CCAGCTCAAA GTTTGAGGAG TTCATGACAA GGCATCAAGT CCATGAGAAG
    GAGGAATTTA AAGCATTAAA AACCCTCAAC ATATTTTACC AAGCTGGCAC ATCTAAGAAC
    GGCAACCCTG TGTTTTATTA CATCGCACGC AGGTTCAAAA CTGGACAAAT CAATGGTGAT
    CTTCTGATCT ACCACGTCCT GCTGACGCTC AAGCCCTACT ATGCCAAGCA TTATGAGATC
    GTAGTTGACC TTACTCATGT GGGCCCCAGT AACCGTTTCA AGACGGACTT CTTATCCAAA
    TGGTTTGTGG TCTTTCCAAA CTTCGCCTAT GAGAACGTGG CTGCTGTGTA CATCTACAAC
    TGCAACACCT GGGTACGCGA GTACACCAAA TACCACGAGC GACTGCTGAC TGGCCTCAAG
    GGGAGCAAGA AGCTGGTGTT TATCGATTCG CCTGCACGTC TAGCTGAACA CATCGAACCC
    GAACAGCAGA AGCTGCCCGC AGCTACTTTA GCACTGGAAG AAGACCTCAA AGTGTTCCAC
    AACGCCCTAA AGCTTGCACA CAGAGACACA AAAGTGTCAA TTAAGGTGGG ATCCACAGCT
    GTACAGGTGA CCTCGGCCGA AAGAACGCGT GTCCTAGGAC AGCCTGTGTT CCTAAATGAC
    ATCTACTACG CTTCAGAGAT CGAGGAGATA TGCCTGGTGG ACGAGAACCA GTTCACGCTA
    ACCATCGCTA ACCAGGGCAC CCCACTCACT TTCATGCACC AGGAGTGTGA AGCCATCGTT
    CAGTCCATCA TCCACATCCG CACGCGCTGG GAACTTTCCC AACCAGACTC CATTCCCCAA
    CACACCAAAA TCAGGCCTAA GGATGTTCCT GGCACCCTGC TCAACATCGC TCTTCTCAAC
    CTGGGAAGCT CCGACCCCAG TCTGAGGTCT GCGGCCTACA ATCTCCTTTG TGCCTTAACC
    TGCACCTTCA ACCTGAAGAT TGAAGGCCAG CTTCTGGAAA CGTCTGGCTT GTGCATCCCA
    GCCAACAACA CGCTTTTTAT TGTGTCCATT AGCAAAACGC TAGCAGCTAA TGAACCCCAC
    CTCACTCTGG AGTTCCTGGA AGAGTGCATC TCAGGATTCA GTAAATCAAG TATAGAGCTG
    AAGCATCTGT GTCTAGAATA TATGACTCCT TGGCTCCGTA ATTTGGTGCG CTTTTGCAAA
    CACAATGATG ACGCCAAACG GCAGCGTGTC ACGGCCATCC TGGACAAGCT TATTACTATG
    ACCATCAACG AGAAGCAGAT GTACCCCTCC ATACAGGCCA AGATATGGGG CAGCTTGGGT
    CAGATCACAG ACCTGCTTGA TGTGGTGTTG GACAGCTTTA TAAAGACCAG CGCTACTGGC
    GGTTTAGGAT CCATCAAAGC TGAAGTGATG GCTGACACTG CTGTAGCTTT GGCGTCAGGA
    AATGTCAAAC TGGTCTCCAG CAAGGTGATT GGCCGAATGT GTAAGATCAT CGACAAGACG
    TGTCTGTCTC CCACTCCGAC CCTTGAGCAG CACCTCATGT GGGACGACAT CGCCATCCTG
    GCCCGGTACA TGCTCATGCT GTCCTTCAAC AACTCTCTGG ACGTGGCCGC TCATCTGCCA
    TACCTGTTCC ACGTCGTCAC CCTGCTGGTG GCCACGGGCC CTCTCTCGCT GCGTGCATCA
    ACACACGGCC TGGTCATCAA CATCATTCAC TCGCTCTGCA CATGCTCACA ACTCAACTTC
    AGTGAGGAGA CTAAACAGGT GCTGCGCCTG AGTCTGACTG AGTTCTCCCT GCCCAAGTTC
    TACCTGCTGT TCGGCATCAG TAAGGTCAAA TCTGCGGCGG TCATCGCTTT CCGTTCCAGC
    TACAGAGACC GCTCATTCTC TCCAGGCTCT TATGAGAGAG AGACATTTGC TCTGTCTTCC
    CTGGAAACAG TCACGGAGGC TCTTTTGGAG ATCATGGAGG CCTGCATGAG GGACATTCCT
    GGATGCAAAT GGCTAGATCA GTGGACTGAA TTGGCGCAAA AGTTTGCGTT CCAGTATAAC
    CCCTCTCTTC AGCCTCGGGC GCTGGTGGTG TTCGGCTGCA TCAGTAAACG AGTGACTCAC
    GGACAGCTAA AGCAGATCAT TCGTATTCTC AGTAAAGCCA GTCCTCTACT CAGCATTGAT
    CGGGGGCTGG AGAGCTGCTT AAAGGGCCCA GATAATTACA ACAGCCAGGT TCTCATCGAA
    GCCACAGTCA TCGCTCTCAC CAAACTACAG CCTCTCCTCG ATCAGGATTC TCCTATGCAT
    AAAGCTCTGT TCTGGGTGGC CGTGGCCGTG CTTCAGTTGG ATGAAGTGAA TCTGTACTCC
    GCCGGCACTG CCTTACTGGA GCAGAACCTG CACACACTGG ACAGCCTCCG AGTGTTTAAT
    GATAAGAGTC CTGAAGAGGT TTTTATGGAG ATCCGGAAAC CTCTAGAGTG GCACTGTAAA
    CAAATGGACC ATTTTGTGGG ACTCAACTTT GCCTCCAACT TCAATTTTGC ATTAGTGGGC
    CATTTACTTA AAGGATACAG ACATCCCTCT TCAACAACAG TTGCCAGGAC AGTTCGTATC
    CTGCATACAC TCCTGGGCCT GGTGGGAAAA CACCGCAGCT GTGACAAGTT TGAGGTGAAC
    ACGCAGAGTG TGGCCTATCT GGCAGCTTTG CTCACAGTGT CAGAGGAAGT TAGGAGCCGC
    TGCAGTTTAA AGCACAGGAA ATCATTGCTG CTTTCAGAAG TTACAATGGA GAACTTCCCC
    ATGGACACAT ACACCGTTTA CGACACTGAA CCCGGGTGCA GGCTACTGCG TGAAAACCAG
    CCTTGGGCTT CTCCGAAAGT GTCAGAGCGC AGTCTTGCAC CTCCGTACTC CACAATAGGG
    CAGACCAGCC TTCGTCCACG CAAATCCATG AGTGTAGACA TGGGCCAGCC GTCTCAGGCC
    AATGCGAAGA AACTACTGGG CACTAGAAAG AGTTTCGATC ACTTAATATC AGAATCCAAA
    GCCCCAAAAA GACCAGACAT GGAGTCGGGC ATCACCACAC CTCCAAAGAT GAGGCGGGTT
    TCTGAAAATG ACTATGAGAT CGAAACTCAG CGAATAGCCA ACTCTCATTT ACGCAAAGTG
    TCTGTGTCTG AATCCAATGT CCTCCTGGAC GAGAACGTAC TTACGGACCC CAAGATCCAG
    ACGTTGCTCC TCACTGTACT GGCAACACTG GTGAAGTACA CTACAGACGA GTTTCATCAG
    CGGATCTTGT ATGAGTATTT GGCTGAGGCC AGTGTGGTCT TCCCCAAGGT CTTTCCAGTT
    GTGCACAACC TGCTGGACTC CAAGTTAAGC ACCGTCCTTT CCATGTGCCA GGACCCGAAC
    CTTCTGAACC CCGTCCATGG CATCATTCAG AACGTGGTCT ATCATGAGGA GTCTCCCCCT
    CAGTACCAGC CGTCCTGCTT GCAGAGCTTT GGTTTCAATG GTCTTTGGAG GTTTGCCGGG
    CCGTTCTCTA AGCAAACCCA AATTCCAGAC TACGCCGAGC TGGTGGTTAA ATTTTTGGAT
    GCTTTGATTG ACACGTATCT CCCGGGCATT GAGGAGGAAG GAGGAGAAGA GTCACTGCGG
    ACCCCGACCT CGCCATATCC ACCAACCGTC CAGAGCCAGC TGAGCATCAC AGCCAATCTC
    AACCTGTCCA ACTCCATGAC ATCACTTGCC ACATCACAGC ACTCCCCAGA GGGCCCTATA
    GTTTATAGAC GGTCCCCAAC TCTCTCCCAT CCACGAACTA CAGAGGAGGT GCCACCGAAA
    ACAGCTGCCT GTCTGCCTTG CTCTAAGTCG GCTGTATTCC CACCTCACCT CGTCCTGCCA
    GGTACTCATC CACCTCTGCT GTGTGAAAGT AGCAGTTGTT GGCATTGGCC CGAGTGTTTG
    AATTGTAAAT CTGCTGCTGT CAGATCAAAG AAAGCAAGAG GGGCTACTAT AACAGATAGT
    GTGAAGAGAG AGCACAAGGG CCTGGTATTA AGCAAACACT TACATACAGT ACATAGCCAG
    GGGACAGGAC ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017206681.1
    CDS253..8727
    Translation

    Target ORF information:

    RefSeq Version XM_017351192.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio neurofibromin 1a (nf1a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351192.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    ATGGCGGCGC ACAAGCCCGT GGAATGGGTC CAGGCCATCA TTAACAGATT CGACGAACAG 
    CTCCCAATCA AAACAGGACA GCAGAGCACC CACACCAAAG TGAGTACAGA GCACAACAAA
    GAATGTTTGA TCAACATCTC CAAGTACAAA TTTGCCCTCG TCATCAATGG CCTGACCAAC
    ATACTCAAAA ATGTGAACAA CATGCGGATA TTTGGCGAAG CTGCTGAGAA GAACCTCTAC
    TTCTCCCAGC TGATCATACT GGACACACTG GAGAAGTGTC TAGCAGGGCA ACCTAAGGAT
    TGCCTGAGGC TGGATGAGGC TATGTTGGTA AAACAGTTGC TGCCTGAAAT CTGTCACTTC
    CTGCACTCGT ACCGTGAGGG CCAGCAACAC GCCACTCAGC TGCGCAGCTC CGCCTCTGCT
    GTGCTTTTCT CTCTCAGCTG CAACAACTTT AATGCCGTCT TCAGTCGTAT CTCCACCAGG
    TTGCAAGAGC TCACAGTTTG TACAGAGGAC ACAGTGGATG TCCATGACAT TGAGCTCATT
    CAGTATATCA ATGTAGATTG CTCCAAGTTA AAGAGACTCC TACAAGAAGC AGTACTTAAG
    TTCAAGTCTT TGAAAAAGCC GGCTCAGCTT GCAGTAATCA GCAGTTTGGA AAAGGCTTTC
    TGGAACTGGG TTGAACACTA CCCTGATGAT TTCACTATGC TCTATCAGAG ACCCCAGGCC
    GACATGGCCG ACTGTGCAGA GAGACTGTTC GACCTAGTGG ACAGTTTTGC GGAAAGCGCA
    AAGAGAAAAG CTGCTGTGTG GCCACTGCAG ATCATCCTCC TCATCCTCTG TCCAGAGATC
    ACGCAGAGCA TCACCAAAGA AGTAGGGGGA GAGGAGAAAG CTAACAAGAA GTCATTTGTA
    GACAGTTTAC GAAAGGCTCT TGGCCAACAC AGCGGCAGCA AACAATTGAC GGAAAGTGCA
    GCTGTTGCAT GCGTCAAACT CTGCAAAGCC TGCACATATA TCAACTGGGA GGATAATTCT
    GTCATCTTTC TCCTGGTTCA GTCCATCGTC ATGGACCTTA AGGGTCTCCT GTTTAACCCC
    ACCAAACCTT TCTCAAGAGG TACGGGCTGC CAGAGCGCCG ATGTAGATTT GATGATCGAC
    TGCTTTGTGT CATGTTATCG CATCAATCCT CGCAACAACC AGCATTTTAA GGTGTGTCTG
    GCCTCGACTT CTCCTCCTAC CTTCCATTTC GTCCTTGTCA ACTCCCTGCA CAAAATTATC
    ACAAATTCTC ACCTGGACTG GTGGCCGAAG ATTGACGCCA TCTACTGCTA CTCAGGAGAA
    CTACGCACCA TGTTTGCAGA CACACTGGGT CGAGTGATTC AGAGTCTCAG TGCACATCCG
    CCTCTGAGGA TGACATCAAG TTTGACATTC AAAGAGAAAA TGACAACAAG TTTGAAGTTC
    AAGGAAAAGT CCACAGATCT TGATTCGCGT TCTTTGCTGC TGGCACTGGT CAAACTTATC
    CACGCTGACC CCAAACTAAT GCTGCACAAT CCGGGAAAAC CTGGTCAGGA GATTCAGAAC
    AGCACAGCTG AGATCATCAA AGGTCTGGTT CAGCTGGTTC CTCTTACCCA CAGTGCTGAG
    CTTTCCCAAG AGGCCATGGA GGCATTGCTT GTTTTACATC AGCCAGAAAC CATTGAGCTA
    TGGAACCCGG ACGCTCCCAT TGAGACCTTC TGGGACATCA GCTCTCAGGT GTTATTTCTA
    ATTTGTAAGA AGCTGATTGG ACACCAGATG ATCAACAGTA CAGAGATACT CAAGTGGCTC
    AGGGAGATTC TCATATGCAG GAACAAGTTT CTATTAAAGA ACAAGGAGTG TGCTACACAG
    GGCAGTCTGA TCCCCATCAG CAGACAGGCT CAGACCAAGC TGGAGGTGTG TCTGTACACG
    TTCCTGTGGA GTCCTGATGC AGAGGCTGTT TTGGTGGCCA TGTCCTGTTT CAGGCATCTC
    TGTGAGGAGG CCGATATTCG CTGCAGTGTC GATGAGCTGC CTGTTCACAC AGTCCTACCC
    AATTACAACA CCTTCATTGA GCTTGCATCC GTCAGCAATA TGATGGCCAC AGGTCGCAAC
    ACACTACAAA AAAGAGTGAT GGCACTTTTA AGAAGAATAA AGCACCCTAC AGCTGGAAAT
    GTAGAGGCAT GGGAAGACAC ATATGCAAAG TGGGAGCAAG CTACAAAACA GATACTCAAC
    CACCCAAAAA ACAAAGTGGA AGATGGACAG GTAGTTAACA TAAGATTGGT CAATTATTTC
    ATCTTTTTTT CCATTCATGG TCATCTATTT TCTGTTCGTC CTCAGGAGTG GATCAACATG
    ACTGGTTTCT TGTGCGCTCT GGGAGGTGTT TGTCTCCAAC AGCGCAGCAC GCCGAGTCTG
    GGCACCTACA GTCCTCCAAT GGGCCCCCTG ACCGAACGCA AGGGGTCCAT GATTTCCATG
    AGCTCCTGCG AGGTAAACAG CGAAACATCC CTGAATCGCT TTCTGGAGCG TCTGCTGGCT
    CTGATGGTCT GCACACATGA GAAAGTGGGA GTGCAAATTC GCACCAACGT CAAAGACCTG
    GTGGGACTGG AGCTCAGTCC GGCTCTCTAT TCAATGCTCT TCACTAAGCT GCGCAACAGC
    ATCGGACGCT TCTTTGACAC CCAGGGGCCG GTTCCTATTA CAGATGCAAA CACTCTGTTT
    GTAGAGCAGA CCATTGCTAT CATGAAGAAC TTGCTGGACA GTCACACTGA AGGAAGCTCA
    GAGCACCTTG GTCAAGCCAG CATTGAGACC ATGATGCTCA ATCTAGTTAG GTATGTGCGT
    ATTTTAGGGA ACATTGTTCA TGCCATTCAG ATCAAAACCA AGTTGTGTCA GTTAGTGGAG
    GTGATGATGG AGCGCAGGGA TGACCTCTCC TTCTGCCAGG AGATGAAATT CAGGAATAAA
    ATGGTGGAGT ACCTCACAGA CTGGGTGATG GGAACCTCCA ATCAAGCTGC GGACGATGAT
    GTCAAATGCC TGACCCGCGA TCTGGACCAG GCCAGTATGG AGGCTGTCGT GTCTCTGTTG
    GCTGGTCTTC CTCTACAGCC TGAGGAGGGT GACGGTGTTG AGCTCATGGA GGCCAAATCA
    CAGCTCTTCC TGAAGTACTT CACACTGTTC ATGAACCTGT TGAATGACTG CAGCGAGGTT
    GAGGAAGATG GCCAGCAGGT CGCAGGGAGG AAACGTGGCA TGTCTAGACG ACTGGCATCT
    CTGCGGCACT GCACAGTATT GGCTATGTCT AACTTGCTCA ATGCTAATGT GGATAGCGGC
    CTCATGCACT CAATCGGTCT GGGATACCAT AAGGACCTGC AGACACGGGC TACATTTATG
    GAAGTGCTCA CTAAGATCCT TCAGCAAGGG ACGGAGTTCG ACACGCTTGC AGAGACTGTG
    CTTGCAGATC GCTTTGAGAG ACTGGTCGAG CTGGTCACCA TGATGGGAGA TCAGGGAGAG
    CTGCCCATAG CCATGGCTCT GGCCAATGTG GTCCCATGCT CTCAATGGGA TGAGCTGGCC
    AGAGTGTTAG TGACTCTCTT CGACTCTCGC CATCTGCTGT ATCAGCTGTT GTGGAATATG
    TTCTCCAAAG AGGTGGAGCT GGCAGACTCA ATGCAGACGC TCTTTAGAGG AAATAGCCTA
    GCCAGCAAGA TTATGACCTT CTGCTTCAAG GTGTACGGAG CTGCGTATCT ACAGAAGCTT
    CTGGAGCCGC TGTTGAGAGG AATCATCACT ACACCAGAGT GGCAGCAGAT CAGTTTTGAA
    GTGGACCCTA CTAGACTTGA ACAGGCAGAG AACCTGGAGG AGAACCAGAA GAACCTGCTT
    CAGATCACTG ACCGCTTCTT CCTGGCCATC ATCAACTCCT CGAGCGAGTT TCCTCCACAG
    CTGCGCAGTG TGTGCCACTG TCTGTATCAG GTGGTCAGTC AGCGGTTTCC TCAGAACAGC
    ATTGGTGCTG TAGGAAGTGC CATGTTCCTG CGCTTTATTA ACCCAGCTAT CGTGTCCCCA
    TATGAGGCTG GAATCCTAGA CAAGAAACCC CCACCTCGCA TCGAGCGTGG CCTTAAACTC
    ATGTCTAAGA TTCTGCAGAG CATTGCTAAT CATGTTCTCT TCACAAAAGA GGAGCATATG
    AGGCCCTTTA ATGATTTTGT CAAAAGCAAC TTCGATGCAG CCAGAAGGTT CTTCTTGGAT
    ATCGCATCTG ATTCTCCACC AAGTGATTCT GTGAACCATA GTCTGTCTTT CATCAGTGAC
    GGTAATGTGC TCGCTCTGCA CCGGCTATTA TGGAACAACC AGGAAAGAAT TGGCCAATAC
    CTGTCCAGCA ACAGGGACCA CAAGGCAGTG GGCCGTAGAC CATTTGACAA GATGGCCACT
    CTTCTGGCGT ACCTGGGTCC TCCTGAACAT AAGCCAGTAG CAGACACACA CTGGTCCAGC
    CTCAACCTCA CCAGCTCAAA GTTTGAGGAG TTCATGACAA GGCATCAAGT CCATGAGAAG
    GAGGAATTTA AAGCATTAAA AACCCTCAAC ATATTTTACC AAGCTGGCAC ATCTAAGAAC
    GGCAACCCTG TGTTTTATTA CATCGCACGC AGGTTCAAAA CTGGACAAAT CAATGGTGAT
    CTTCTGATCT ACCACGTCCT GCTGACGCTC AAGCCCTACT ATGCCAAGCA TTATGAGATC
    GTAGTTGACC TTACTCATGT GGGCCCCAGT AACCGTTTCA AGACGGACTT CTTATCCAAA
    TGGTTTGTGG TCTTTCCAAA CTTCGCCTAT GAGAACGTGG CTGCTGTGTA CATCTACAAC
    TGCAACACCT GGGTACGCGA GTACACCAAA TACCACGAGC GACTGCTGAC TGGCCTCAAG
    GGGAGCAAGA AGCTGGTGTT TATCGATTCG CCTGCACGTC TAGCTGAACA CATCGAACCC
    GAACAGCAGA AGCTGCCCGC AGCTACTTTA GCACTGGAAG AAGACCTCAA AGTGTTCCAC
    AACGCCCTAA AGCTTGCACA CAGAGACACA AAAGTGTCAA TTAAGGTGGG ATCCACAGCT
    GTACAGGTGA CCTCGGCCGA AAGAACGCGT GTCCTAGGAC AGCCTGTGTT CCTAAATGAC
    ATCTACTACG CTTCAGAGAT CGAGGAGATA TGCCTGGTGG ACGAGAACCA GTTCACGCTA
    ACCATCGCTA ACCAGGGCAC CCCACTCACT TTCATGCACC AGGAGTGTGA AGCCATCGTT
    CAGTCCATCA TCCACATCCG CACGCGCTGG GAACTTTCCC AACCAGACTC CATTCCCCAA
    CACACCAAAA TCAGGCCTAA GGATGTTCCT GGCACCCTGC TCAACATCGC TCTTCTCAAC
    CTGGGAAGCT CCGACCCCAG TCTGAGGTCT GCGGCCTACA ATCTCCTTTG TGCCTTAACC
    TGCACCTTCA ACCTGAAGAT TGAAGGCCAG CTTCTGGAAA CGTCTGGCTT GTGCATCCCA
    GCCAACAACA CGCTTTTTAT TGTGTCCATT AGCAAAACGC TAGCAGCTAA TGAACCCCAC
    CTCACTCTGG AGTTCCTGGA AGAGTGCATC TCAGGATTCA GTAAATCAAG TATAGAGCTG
    AAGCATCTGT GTCTAGAATA TATGACTCCT TGGCTCCGTA ATTTGGTGCG CTTTTGCAAA
    CACAATGATG ACGCCAAACG GCAGCGTGTC ACGGCCATCC TGGACAAGCT TATTACTATG
    ACCATCAACG AGAAGCAGAT GTACCCCTCC ATACAGGCCA AGATATGGGG CAGCTTGGGT
    CAGATCACAG ACCTGCTTGA TGTGGTGTTG GACAGCTTTA TAAAGACCAG CGCTACTGGC
    GGTTTAGGAT CCATCAAAGC TGAAGTGATG GCTGACACTG CTGTAGCTTT GGCGTCAGGA
    AATGTCAAAC TGGTCTCCAG CAAGGTGATT GGCCGAATGT GTAAGATCAT CGACAAGACG
    TGTCTGTCTC CCACTCCGAC CCTTGAGCAG CACCTCATGT GGGACGACAT CGCCATCCTG
    GCCCGGTACA TGCTCATGCT GTCCTTCAAC AACTCTCTGG ACGTGGCCGC TCATCTGCCA
    TACCTGTTCC ACGTCGTCAC CCTGCTGGTG GCCACGGGCC CTCTCTCGCT GCGTGCATCA
    ACACACGGCC TGGTCATCAA CATCATTCAC TCGCTCTGCA CATGCTCACA ACTCAACTTC
    AGTGAGGAGA CTAAACAGGT GCTGCGCCTG AGTCTGACTG AGTTCTCCCT GCCCAAGTTC
    TACCTGCTGT TCGGCATCAG TAAGGTCAAA TCTGCGGCGG TCATCGCTTT CCGTTCCAGC
    TACAGAGACC GCTCATTCTC TCCAGGCTCT TATGAGAGAG AGACATTTGC TCTGTCTTCC
    CTGGAAACAG TCACGGAGGC TCTTTTGGAG ATCATGGAGG CCTGCATGAG GGACATTCCT
    GGATGCAAAT GGCTAGATCA GTGGACTGAA TTGGCGCAAA AGTTTGCGTT CCAGTATAAC
    CCCTCTCTTC AGCCTCGGGC GCTGGTGGTG TTCGGCTGCA TCAGTAAACG AGTGACTCAC
    GGACAGCTAA AGCAGATCAT TCGTATTCTC AGTAAAGCCA GTCCTCTACT CAGCATTGAT
    CGGGGGCTGG AGAGCTGCTT AAAGGGCCCA GATAATTACA ACAGCCAGGT TCTCATCGAA
    GCCACAGTCA TCGCTCTCAC CAAACTACAG CCTCTCCTCG ATCAGGATTC TCCTATGCAT
    AAAGCTCTGT TCTGGGTGGC CGTGGCCGTG CTTCAGTTGG ATGAAGTGAA TCTGTACTCC
    GCCGGCACTG CCTTACTGGA GCAGAACCTG CACACACTGG ACAGCCTCCG AGTGTTTAAT
    GATAAGAGTC CTGAAGAGGT TTTTATGGAG ATCCGGAAAC CTCTAGAGTG GCACTGTAAA
    CAAATGGACC ATTTTGTGGG ACTCAACTTT GCCTCCAACT TCAATTTTGC ATTAGTGGGC
    CATTTACTTA AAGGATACAG ACATCCCTCT TCAACAACAG TTGCCAGGAC AGTTCGTATC
    CTGCATACAC TCCTGGGCCT GGTGGGAAAA CACCGCAGCT GTGACAAGTT TGAGGTGAAC
    ACGCAGAGTG TGGCCTATCT GGCAGCTTTG CTCACAGTGT CAGAGGAAGT TAGGAGCCGC
    TGCAGTTTAA AGCACAGGAA ATCATTGCTG CTTTCAGAAG TTACAATGGA GAACTTCCCC
    ATGGACACAT ACACCGTTTA CGACACTGAA CCCGGGTGCA GGCTACTGCG TGAAAACCAG
    CCTTGGGCTT CTCCGAAAGT GTCAGAGCGC AGTCTTGCAC CTCCGTACTC CACAATAGGG
    CAGACCAGCC TTCGTCCACG CAAATCCATG AGTGTAGACA TGGGCCAGCC GTCTCAGGCC
    AATGCGAAGA AACTACTGGG CACTAGAAAG AGTTTCGATC ACTTAATATC AGAATCCAAA
    GCCCCAAAAA GACCAGACAT GGAGTCGGGC ATCACCACAC CTCCAAAGAT GAGGCGGGTT
    TCTGAAAATG ACTATGAGAT CGAAACTCAG CGAATAGCCA ACTCTCATTT ACGCAAAGTG
    TCTGTGTCTG AATCCAATGT CCTCCTGGAC GAGAACGTAC TTACGGACCC CAAGATCCAG
    ACGTTGCTCC TCACTGTACT GGCAACACTG GTGAAGTACA CTACAGACGA GTTTCATCAG
    CGGATCTTGT ATGAGTATTT GGCTGAGGCC AGTGTGGTCT TCCCCAAGGT CTTTCCAGTT
    GTGCACAACC TGCTGGACTC CAAGTTAAGC ACCGTCCTTT CCATGTGCCA GGACCCGAAC
    CTTCTGAACC CCGTCCATGG CATCATTCAG AACGTGGTCT ATCATGAGGA GTCTCCCCCT
    CAGTACCAGC CGTCCTGCTT GCAGAGCTTT GGTTTCAATG GTCTTTGGAG GTTTGCCGGG
    CCGTTCTCTA AGCAAACCCA AATTCCAGAC TACGCCGAGC TGGTGGTTAA ATTTTTGGAT
    GCTTTGATTG ACACGTATCT CCCGGGCATT GAGGAGGAAG GAGGAGAAGA GTCACTGCGG
    ACCCCGACCT CGCCATATCC ACCAACCGTC CAGAGCCAGC TGAGCATCAC AGCCAATCTC
    AACCTGTCCA ACTCCATGAC ATCACTTGCC ACATCACAGC ACTCCCCAGA GGGCCCTATA
    GTTTATAGAC GGTCCCCAAC TCTCTCCCAT CCACGAACTA CAGAGGAGGT GCCACCGAAA
    ACAGCTGCCT GTCTGCCTTG CTCTAAGTCG GCTGTATTCC CACCTCACCT CGTCCTGCCA
    GGTACTCATC CACCTCTGCT GTGTGAAAGT AGCAGTTGTT GGCATTGGCC CGAGTGTTTG
    AATTGTAAAT CTGCTGCTGT CAGATCAAAG AAAGCAAGAG GGGCTACTAT AACAGATAGT
    GTGAAGAGAG AGCACAAGGG CCTGGTATTA AGCAAACACT TACATACAGT ACATAGCCAG
    GGGACAGGAC ATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55270
    Clone ID Related Accession (Same CDS sequence) XM_017351193.1
    Accession Version XM_017351193.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8409bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product neurofibromin isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
    1081
    1141
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    1261
    1321
    1381
    1441
    1501
    1561
    1621
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    1981
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    7921
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    8401
    ATGGCGGCGC ACAAGCCCGT GGAATGGGTC CAGGCCATCA TTAACAGATT CGACGAACAG 
    CTCCCAATCA AAACAGGACA GCAGAGCACC CACACCAAAG TGAGTACAGA GCACAACAAA
    GAATGTTTGA TCAACATCTC CAAGTACAAA TTTGCCCTCG TCATCAATGG CCTGACCAAC
    ATACTCAAAA ATGTGAACAA CATGCGGATA TTTGGCGAAG CTGCTGAGAA GAACCTCTAC
    TTCTCCCAGC TGATCATACT GGACACACTG GAGAAGTGTC TAGCAGGGCA ACCTAAGGAT
    TGCCTGAGGC TGGATGAGGC TATGTTGGTA AAACAGTTGC TGCCTGAAAT CTGTCACTTC
    CTGCACTCGT ACCGTGAGGG CCAGCAACAC GCCACTCAGC TGCGCAGCTC CGCCTCTGCT
    GTGCTTTTCT CTCTCAGCTG CAACAACTTT AATGCCGTCT TCAGTCGTAT CTCCACCAGG
    TTGCAAGAGC TCACAGTTTG TACAGAGGAC ACAGTGGATG TCCATGACAT TGAGCTCATT
    CAGTATATCA ATGTAGATTG CTCCAAGTTA AAGAGACTCC TACAAGAAGC AGTACTTAAG
    TTCAAGTCTT TGAAAAAGCC GGCTCAGCTT GCAGTAATCA GCAGTTTGGA AAAGGCTTTC
    TGGAACTGGG TTGAACACTA CCCTGATGAT TTCACTATGC TCTATCAGAG ACCCCAGGCC
    GACATGGCCG ACTGTGCAGA GAGACTGTTC GACCTAGTGG ACAGTTTTGC GGAAAGCGCA
    AAGAGAAAAG CTGCTGTGTG GCCACTGCAG ATCATCCTCC TCATCCTCTG TCCAGAGATC
    ACGCAGAGCA TCACCAAAGA AGTAGGGGGA GAGGAGAAAG CTAACAAGAA GTCATTTGTA
    GACAGTTTAC GAAAGGCTCT TGGCCAACAC AGCGGCAGCA AACAATTGAC GGAAAGTGCA
    GCTGTTGCAT GCGTCAAACT CTGCAAAGCC TGCACATATA TCAACTGGGA GGATAATTCT
    GTCATCTTTC TCCTGGTTCA GTCCATCGTC ATGGACCTTA AGGGTCTCCT GTTTAACCCC
    ACCAAACCTT TCTCAAGAGG TACGGGCTGC CAGAGCGCCG ATGTAGATTT GATGATCGAC
    TGCTTTGTGT CATGTTATCG CATCAATCCT CGCAACAACC AGCATTTTAA GGTGTGTCTG
    GCCTCGACTT CTCCTCCTAC CTTCCATTTC GTCCTTGTCA ACTCCCTGCA CAAAATTATC
    ACAAATTCTC ACCTGGACTG GTGGCCGAAG ATTGACGCCA TCTACTGCTA CTCAGGAGAA
    CTACGCACCA TGTTTGCAGA CACACTGGGT CGAGTGATTC AGAGTCTCAG TGCACATCCG
    CCTCTGAGGA TGACATCAAG TTTGACATTC AAAGAGAAAA TGACAACAAG TTTGAAGTTC
    AAGGAAAAGT CCACAGATCT TGATTCGCGT TCTTTGCTGC TGGCACTGGT CAAACTTATC
    CACGCTGACC CCAAACTAAT GCTGCACAAT CCGGGAAAAC CTGGTCAGGA GATTCAGAAC
    AGCACAGCTG AGATCATCAA AGGTCTGGTT CAGCTGGTTC CTCTTACCCA CAGTGCTGAG
    CTTTCCCAAG AGGCCATGGA GGCATTGCTT GTTTTACATC AGCCAGAAAC CATTGAGCTA
    TGGAACCCGG ACGCTCCCAT TGAGACCTTC TGGGACATCA GCTCTCAGGT GTTATTTCTA
    ATTTGTAAGA AGCTGATTGG ACACCAGATG ATCAACAGTA CAGAGATACT CAAGTGGCTC
    AGGGAGATTC TCATATGCAG GAACAAGTTT CTATTAAAGA ACAAGGAGTG TGCTACACAG
    GGCAGTCTGA TCCCCATCAG CAGACAGGCT CAGACCAAGC TGGAGGTGTG TCTGTACACG
    TTCCTGTGGA GTCCTGATGC AGAGGCTGTT TTGGTGGCCA TGTCCTGTTT CAGGCATCTC
    TGTGAGGAGG CCGATATTCG CTGCAGTGTC GATGAGCTGC CTGTTCACAC AGTCCTACCC
    AATTACAACA CCTTCATTGA GCTTGCATCC GTCAGCAATA TGATGGCCAC AGGTCGCAAC
    ACACTACAAA AAAGAGTGAT GGCACTTTTA AGAAGAATAA AGCACCCTAC AGCTGGAAAT
    GTAGAGGCAT GGGAAGACAC ATATGCAAAG TGGGAGCAAG CTACAAAACA GATACTCAAC
    CACCCAAAAA ACAAAGTGGA AGATGGACAG GAGTGGATCA ACATGACTGG TTTCTTGTGC
    GCTCTGGGAG GTGTTTGTCT CCAACAGCGC AGCACGCCGA GTCTGGGCAC CTACAGTCCT
    CCAATGGGCC CCCTGACCGA ACGCAAGGGG TCCATGATTT CCATGAGCTC CTGCGAGGTA
    AACAGCGAAA CATCCCTGAA TCGCTTTCTG GAGCGTCTGC TGGCTCTGAT GGTCTGCACA
    CATGAGAAAG TGGGAGTGCA AATTCGCACC AACGTCAAAG ACCTGGTGGG ACTGGAGCTC
    AGTCCGGCTC TCTATTCAAT GCTCTTCACT AAGCTGCGCA ACAGCATCGG ACGCTTCTTT
    GACACCCAGG GGCCGGTTCC TATTACAGAT GCAAACACTC TGTTTGTAGA GCAGACCATT
    GCTATCATGA AGAACTTGCT GGACAGTCAC ACTGAAGGAA GCTCAGAGCA CCTTGGTCAA
    GCCAGCATTG AGACCATGAT GCTCAATCTA GTTAGGTATG TGCGTATTTT AGGGAACATT
    GTTCATGCCA TTCAGATCAA AACCAAGTTG TGTCAGTTAG TGGAGGTGAT GATGGAGCGC
    AGGGATGACC TCTCCTTCTG CCAGGAGATG AAATTCAGGA ATAAAATGGT GGAGTACCTC
    ACAGACTGGG TGATGGGAAC CTCCAATCAA GCTGCGGACG ATGATGTCAA ATGCCTGACC
    CGCGATCTGG ACCAGGCCAG TATGGAGGCT GTCGTGTCTC TGTTGGCTGG TCTTCCTCTA
    CAGCCTGAGG AGGGTGACGG TGTTGAGCTC ATGGAGGCCA AATCACAGCT CTTCCTGAAG
    TACTTCACAC TGTTCATGAA CCTGTTGAAT GACTGCAGCG AGGTTGAGGA AGATGGCCAG
    CAGGTCGCAG GGAGGAAACG TGGCATGTCT AGACGACTGG CATCTCTGCG GCACTGCACA
    GTATTGGCTA TGTCTAACTT GCTCAATGCT AATGTGGATA GCGGCCTCAT GCACTCAATC
    GGTCTGGGAT ACCATAAGGA CCTGCAGACA CGGGCTACAT TTATGGAAGT GCTCACTAAG
    ATCCTTCAGC AAGGGACGGA GTTCGACACG CTTGCAGAGA CTGTGCTTGC AGATCGCTTT
    GAGAGACTGG TCGAGCTGGT CACCATGATG GGAGATCAGG GAGAGCTGCC CATAGCCATG
    GCTCTGGCCA ATGTGGTCCC ATGCTCTCAA TGGGATGAGC TGGCCAGAGT GTTAGTGACT
    CTCTTCGACT CTCGCCATCT GCTGTATCAG CTGTTGTGGA ATATGTTCTC CAAAGAGGTG
    GAGCTGGCAG ACTCAATGCA GACGCTCTTT AGAGGAAATA GCCTAGCCAG CAAGATTATG
    ACCTTCTGCT TCAAGGTGTA CGGAGCTGCG TATCTACAGA AGCTTCTGGA GCCGCTGTTG
    AGAGGAATCA TCACTACACC AGAGTGGCAG CAGATCAGTT TTGAAGTGGA CCCTACTAGA
    CTTGAACAGG CAGAGAACCT GGAGGAGAAC CAGAAGAACC TGCTTCAGAT CACTGACCGC
    TTCTTCCTGG CCATCATCAA CTCCTCGAGC GAGTTTCCTC CACAGCTGCG CAGTGTGTGC
    CACTGTCTGT ATCAGGTGGT CAGTCAGCGG TTTCCTCAGA ACAGCATTGG TGCTGTAGGA
    AGTGCCATGT TCCTGCGCTT TATTAACCCA GCTATCGTGT CCCCATATGA GGCTGGAATC
    CTAGACAAGA AACCCCCACC TCGCATCGAG CGTGGCCTTA AACTCATGTC TAAGATTCTG
    CAGAGCATTG CTAATCATGT TCTCTTCACA AAAGAGGAGC ATATGAGGCC CTTTAATGAT
    TTTGTCAAAA GCAACTTCGA TGCAGCCAGA AGGTTCTTCT TGGATATCGC ATCTGATTCT
    CCACCAAGTG ATTCTGTGAA CCATAGTCTG TCTTTCATCA GTGACGGTAA TGTGCTCGCT
    CTGCACCGGC TATTATGGAA CAACCAGGAA AGAATTGGCC AATACCTGTC CAGCAACAGG
    GACCACAAGG CAGTGGGCCG TAGACCATTT GACAAGATGG CCACTCTTCT GGCGTACCTG
    GGTCCTCCTG AACATAAGCC AGTAGCAGAC ACACACTGGT CCAGCCTCAA CCTCACCAGC
    TCAAAGTTTG AGGAGTTCAT GACAAGGCAT CAAGTCCATG AGAAGGAGGA ATTTAAAGCA
    TTAAAAACCC TCAACATATT TTACCAAGCT GGCACATCTA AGAACGGCAA CCCTGTGTTT
    TATTACATCG CACGCAGGTT CAAAACTGGA CAAATCAATG GTGATCTTCT GATCTACCAC
    GTCCTGCTGA CGCTCAAGCC CTACTATGCC AAGCATTATG AGATCGTAGT TGACCTTACT
    CATGTGGGCC CCAGTAACCG TTTCAAGACG GACTTCTTAT CCAAATGGTT TGTGGTCTTT
    CCAAACTTCG CCTATGAGAA CGTGGCTGCT GTGTACATCT ACAACTGCAA CACCTGGGTA
    CGCGAGTACA CCAAATACCA CGAGCGACTG CTGACTGGCC TCAAGGGGAG CAAGAAGCTG
    GTGTTTATCG ATTCGCCTGC ACGTCTAGCT GAACACATCG AACCCGAACA GCAGAAGCTG
    CCCGCAGCTA CTTTAGCACT GGAAGAAGAC CTCAAAGTGT TCCACAACGC CCTAAAGCTT
    GCACACAGAG ACACAAAAGT GTCAATTAAG GTGGGATCCA CAGCTGTACA GGTGACCTCG
    GCCGAAAGAA CGCGTGTCCT AGGACAGCCT GTGTTCCTAA ATGACATCTA CTACGCTTCA
    GAGATCGAGG AGATATGCCT GGTGGACGAG AACCAGTTCA CGCTAACCAT CGCTAACCAG
    GGCACCCCAC TCACTTTCAT GCACCAGGAG TGTGAAGCCA TCGTTCAGTC CATCATCCAC
    ATCCGCACGC GCTGGGAACT TTCCCAACCA GACTCCATTC CCCAACACAC CAAAATCAGG
    CCTAAGGATG TTCCTGGCAC CCTGCTCAAC ATCGCTCTTC TCAACCTGGG AAGCTCCGAC
    CCCAGTCTGA GGTCTGCGGC CTACAATCTC CTTTGTGCCT TAACCTGCAC CTTCAACCTG
    AAGATTGAAG GCCAGCTTCT GGAAACGTCT GGCTTGTGCA TCCCAGCCAA CAACACGCTT
    TTTATTGTGT CCATTAGCAA AACGCTAGCA GCTAATGAAC CCCACCTCAC TCTGGAGTTC
    CTGGAAGAGT GCATCTCAGG ATTCAGTAAA TCAAGTATAG AGCTGAAGCA TCTGTGTCTA
    GAATATATGA CTCCTTGGCT CCGTAATTTG GTGCGCTTTT GCAAACACAA TGATGACGCC
    AAACGGCAGC GTGTCACGGC CATCCTGGAC AAGCTTATTA CTATGACCAT CAACGAGAAG
    CAGATGTACC CCTCCATACA GGCCAAGATA TGGGGCAGCT TGGGTCAGAT CACAGACCTG
    CTTGATGTGG TGTTGGACAG CTTTATAAAG ACCAGCGCTA CTGGCGGTTT AGGATCCATC
    AAAGCTGAAG TGATGGCTGA CACTGCTGTA GCTTTGGCGT CAGGAAATGT CAAACTGGTC
    TCCAGCAAGG TGATTGGCCG AATGTGTAAG ATCATCGACA AGACGTGTCT GTCTCCCACT
    CCGACCCTTG AGCAGCACCT CATGTGGGAC GACATCGCCA TCCTGGCCCG GTACATGCTC
    ATGCTGTCCT TCAACAACTC TCTGGACGTG GCCGCTCATC TGCCATACCT GTTCCACGTC
    GTCACCCTGC TGGTGGCCAC GGGCCCTCTC TCGCTGCGTG CATCAACACA CGGCCTGGTC
    ATCAACATCA TTCACTCGCT CTGCACATGC TCACAACTCA ACTTCAGTGA GGAGACTAAA
    CAGGTGCTGC GCCTGAGTCT GACTGAGTTC TCCCTGCCCA AGTTCTACCT GCTGTTCGGC
    ATCAGTAAGG TCAAATCTGC GGCGGTCATC GCTTTCCGTT CCAGCTACAG AGACCGCTCA
    TTCTCTCCAG GCTCTTATGA GAGAGAGACA TTTGCTCTGT CTTCCCTGGA AACAGTCACG
    GAGGCTCTTT TGGAGATCAT GGAGGCCTGC ATGAGGGACA TTCCTGGATG CAAATGGCTA
    GATCAGTGGA CTGAATTGGC GCAAAAGTTT GCGTTCCAGT ATAACCCCTC TCTTCAGCCT
    CGGGCGCTGG TGGTGTTCGG CTGCATCAGT AAACGAGTGA CTCACGGACA GCTAAAGCAG
    ATCATTCGTA TTCTCAGTAA AGCCAGTCCT CTACTCAGCA TTGATCGGGG GCTGGAGAGC
    TGCTTAAAGG GCCCAGATAA TTACAACAGC CAGGTTCTCA TCGAAGCCAC AGTCATCGCT
    CTCACCAAAC TACAGCCTCT CCTCGATCAG GATTCTCCTA TGCATAAAGC TCTGTTCTGG
    GTGGCCGTGG CCGTGCTTCA GTTGGATGAA GTGAATCTGT ACTCCGCCGG CACTGCCTTA
    CTGGAGCAGA ACCTGCACAC ACTGGACAGC CTCCGAGTGT TTAATGATAA GAGTCCTGAA
    GAGGTTTTTA TGGAGATCCG GAAACCTCTA GAGTGGCACT GTAAACAAAT GGACCATTTT
    GTGGGACTCA ACTTTGCCTC CAACTTCAAT TTTGCATTAG TGGGCCATTT ACTTAAAGGA
    TACAGACATC CCTCTTCAAC AACAGTTGCC AGGACAGTTC GTATCCTGCA TACACTCCTG
    GGCCTGGTGG GAAAACACCG CAGCTGTGAC AAGTTTGAGG TGAACACGCA GAGTGTGGCC
    TATCTGGCAG CTTTGCTCAC AGTGTCAGAG GAAGTTAGGA GCCGCTGCAG TTTAAAGCAC
    AGGAAATCAT TGCTGCTTTC AGAAGTTACA ATGGAGAACT TCCCCATGGA CACATACACC
    GTTTACGACA CTGAACCCGG GTGCAGGCTA CTGCGTGAAA ACCAGCCTTG GGCTTCTCCG
    AAAGTGTCAG AGCGCAGTCT TGCACCTCCG TACTCCACAA TAGGGCAGAC CAGCCTTCGT
    CCACGCAAAT CCATGAGTGT AGACATGGGC CAGCCGTCTC AGGCCAATGC GAAGAAACTA
    CTGGGTATTA GCGGCACTAG AAAGAGTTTC GATCACTTAA TATCAGAATC CAAAGCCCCA
    AAAAGACCAG ACATGGAGTC GGGCATCACC ACACCTCCAA AGATGAGGCG GGTTTCTGAA
    AATGACTATG AGATCGAAAC TCAGCGAATA GCCAACTCTC ATTTACGCAA AGTGTCTGTG
    TCTGAATCCA ATGTCCTCCT GGACGAGAAC GTACTTACGG ACCCCAAGAT CCAGACGTTG
    CTCCTCACTG TACTGGCAAC ACTGGTGAAG TACACTACAG ACGAGTTTCA TCAGCGGATC
    TTGTATGAGT ATTTGGCTGA GGCCAGTGTG GTCTTCCCCA AGGTCTTTCC AGTTGTGCAC
    AACCTGCTGG ACTCCAAGTT AAGCACCGTC CTTTCCATGT GCCAGGACCC GAACCTTCTG
    AACCCCGTCC ATGGCATCAT TCAGAACGTG GTCTATCATG AGGAGTCTCC CCCTCAGTAC
    CAGCCGTCCT GCTTGCAGAG CTTTGGTTTC AATGGTCTTT GGAGGTTTGC CGGGCCGTTC
    TCTAAGCAAA CCCAAATTCC AGACTACGCC GAGCTGGTGG TTAAATTTTT GGATGCTTTG
    ATTGACACGT ATCTCCCGGG CATTGAGGAG GAAGGAGGAG AAGAGTCACT GCGGACCCCG
    ACCTCGCCAT ATCCACCAAC CGTCCAGAGC CAGCTGAGCA TCACAGCCAA TCTCAACCTG
    TCCAACTCCA TGACATCACT TGCCACATCA CAGCACTCCC CAGAGGGCCC TATAGTTTAT
    AGACGGTCCC CAACTCTCTC CCATCCACGA ACTACAGAGG AGGTGCCACC GAAAACAGCT
    GCCTGTCTGC CTTGCTCTAA GTCGGCTGTA TTCCCACCTC ACCTCGTCCT GCCAGGTACT
    CATCCACCTC TGCTGTGTGA AAGTAGCAGT TGTTGGCATT GGCCCGAGTG TTTGAATTGT
    AAATCTGCTG CTGTCAGATC AAAGAAAGCA AGAGGGGCTA CTATAACAGA TAGTGTGAAG
    AGAGAGCACA AGGGCCTGGT ATTAAGCAAA CACTTACATA CAGTACATAG CCAGGGGACA
    GGACATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017206682.1
    CDS253..8661
    Translation

    Target ORF information:

    RefSeq Version XM_017351193.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio neurofibromin 1a (nf1a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351193.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGGCGGCGC ACAAGCCCGT GGAATGGGTC CAGGCCATCA TTAACAGATT CGACGAACAG 
    CTCCCAATCA AAACAGGACA GCAGAGCACC CACACCAAAG TGAGTACAGA GCACAACAAA
    GAATGTTTGA TCAACATCTC CAAGTACAAA TTTGCCCTCG TCATCAATGG CCTGACCAAC
    ATACTCAAAA ATGTGAACAA CATGCGGATA TTTGGCGAAG CTGCTGAGAA GAACCTCTAC
    TTCTCCCAGC TGATCATACT GGACACACTG GAGAAGTGTC TAGCAGGGCA ACCTAAGGAT
    TGCCTGAGGC TGGATGAGGC TATGTTGGTA AAACAGTTGC TGCCTGAAAT CTGTCACTTC
    CTGCACTCGT ACCGTGAGGG CCAGCAACAC GCCACTCAGC TGCGCAGCTC CGCCTCTGCT
    GTGCTTTTCT CTCTCAGCTG CAACAACTTT AATGCCGTCT TCAGTCGTAT CTCCACCAGG
    TTGCAAGAGC TCACAGTTTG TACAGAGGAC ACAGTGGATG TCCATGACAT TGAGCTCATT
    CAGTATATCA ATGTAGATTG CTCCAAGTTA AAGAGACTCC TACAAGAAGC AGTACTTAAG
    TTCAAGTCTT TGAAAAAGCC GGCTCAGCTT GCAGTAATCA GCAGTTTGGA AAAGGCTTTC
    TGGAACTGGG TTGAACACTA CCCTGATGAT TTCACTATGC TCTATCAGAG ACCCCAGGCC
    GACATGGCCG ACTGTGCAGA GAGACTGTTC GACCTAGTGG ACAGTTTTGC GGAAAGCGCA
    AAGAGAAAAG CTGCTGTGTG GCCACTGCAG ATCATCCTCC TCATCCTCTG TCCAGAGATC
    ACGCAGAGCA TCACCAAAGA AGTAGGGGGA GAGGAGAAAG CTAACAAGAA GTCATTTGTA
    GACAGTTTAC GAAAGGCTCT TGGCCAACAC AGCGGCAGCA AACAATTGAC GGAAAGTGCA
    GCTGTTGCAT GCGTCAAACT CTGCAAAGCC TGCACATATA TCAACTGGGA GGATAATTCT
    GTCATCTTTC TCCTGGTTCA GTCCATCGTC ATGGACCTTA AGGGTCTCCT GTTTAACCCC
    ACCAAACCTT TCTCAAGAGG TACGGGCTGC CAGAGCGCCG ATGTAGATTT GATGATCGAC
    TGCTTTGTGT CATGTTATCG CATCAATCCT CGCAACAACC AGCATTTTAA GGTGTGTCTG
    GCCTCGACTT CTCCTCCTAC CTTCCATTTC GTCCTTGTCA ACTCCCTGCA CAAAATTATC
    ACAAATTCTC ACCTGGACTG GTGGCCGAAG ATTGACGCCA TCTACTGCTA CTCAGGAGAA
    CTACGCACCA TGTTTGCAGA CACACTGGGT CGAGTGATTC AGAGTCTCAG TGCACATCCG
    CCTCTGAGGA TGACATCAAG TTTGACATTC AAAGAGAAAA TGACAACAAG TTTGAAGTTC
    AAGGAAAAGT CCACAGATCT TGATTCGCGT TCTTTGCTGC TGGCACTGGT CAAACTTATC
    CACGCTGACC CCAAACTAAT GCTGCACAAT CCGGGAAAAC CTGGTCAGGA GATTCAGAAC
    AGCACAGCTG AGATCATCAA AGGTCTGGTT CAGCTGGTTC CTCTTACCCA CAGTGCTGAG
    CTTTCCCAAG AGGCCATGGA GGCATTGCTT GTTTTACATC AGCCAGAAAC CATTGAGCTA
    TGGAACCCGG ACGCTCCCAT TGAGACCTTC TGGGACATCA GCTCTCAGGT GTTATTTCTA
    ATTTGTAAGA AGCTGATTGG ACACCAGATG ATCAACAGTA CAGAGATACT CAAGTGGCTC
    AGGGAGATTC TCATATGCAG GAACAAGTTT CTATTAAAGA ACAAGGAGTG TGCTACACAG
    GGCAGTCTGA TCCCCATCAG CAGACAGGCT CAGACCAAGC TGGAGGTGTG TCTGTACACG
    TTCCTGTGGA GTCCTGATGC AGAGGCTGTT TTGGTGGCCA TGTCCTGTTT CAGGCATCTC
    TGTGAGGAGG CCGATATTCG CTGCAGTGTC GATGAGCTGC CTGTTCACAC AGTCCTACCC
    AATTACAACA CCTTCATTGA GCTTGCATCC GTCAGCAATA TGATGGCCAC AGGTCGCAAC
    ACACTACAAA AAAGAGTGAT GGCACTTTTA AGAAGAATAA AGCACCCTAC AGCTGGAAAT
    GTAGAGGCAT GGGAAGACAC ATATGCAAAG TGGGAGCAAG CTACAAAACA GATACTCAAC
    CACCCAAAAA ACAAAGTGGA AGATGGACAG GAGTGGATCA ACATGACTGG TTTCTTGTGC
    GCTCTGGGAG GTGTTTGTCT CCAACAGCGC AGCACGCCGA GTCTGGGCAC CTACAGTCCT
    CCAATGGGCC CCCTGACCGA ACGCAAGGGG TCCATGATTT CCATGAGCTC CTGCGAGGTA
    AACAGCGAAA CATCCCTGAA TCGCTTTCTG GAGCGTCTGC TGGCTCTGAT GGTCTGCACA
    CATGAGAAAG TGGGAGTGCA AATTCGCACC AACGTCAAAG ACCTGGTGGG ACTGGAGCTC
    AGTCCGGCTC TCTATTCAAT GCTCTTCACT AAGCTGCGCA ACAGCATCGG ACGCTTCTTT
    GACACCCAGG GGCCGGTTCC TATTACAGAT GCAAACACTC TGTTTGTAGA GCAGACCATT
    GCTATCATGA AGAACTTGCT GGACAGTCAC ACTGAAGGAA GCTCAGAGCA CCTTGGTCAA
    GCCAGCATTG AGACCATGAT GCTCAATCTA GTTAGGTATG TGCGTATTTT AGGGAACATT
    GTTCATGCCA TTCAGATCAA AACCAAGTTG TGTCAGTTAG TGGAGGTGAT GATGGAGCGC
    AGGGATGACC TCTCCTTCTG CCAGGAGATG AAATTCAGGA ATAAAATGGT GGAGTACCTC
    ACAGACTGGG TGATGGGAAC CTCCAATCAA GCTGCGGACG ATGATGTCAA ATGCCTGACC
    CGCGATCTGG ACCAGGCCAG TATGGAGGCT GTCGTGTCTC TGTTGGCTGG TCTTCCTCTA
    CAGCCTGAGG AGGGTGACGG TGTTGAGCTC ATGGAGGCCA AATCACAGCT CTTCCTGAAG
    TACTTCACAC TGTTCATGAA CCTGTTGAAT GACTGCAGCG AGGTTGAGGA AGATGGCCAG
    CAGGTCGCAG GGAGGAAACG TGGCATGTCT AGACGACTGG CATCTCTGCG GCACTGCACA
    GTATTGGCTA TGTCTAACTT GCTCAATGCT AATGTGGATA GCGGCCTCAT GCACTCAATC
    GGTCTGGGAT ACCATAAGGA CCTGCAGACA CGGGCTACAT TTATGGAAGT GCTCACTAAG
    ATCCTTCAGC AAGGGACGGA GTTCGACACG CTTGCAGAGA CTGTGCTTGC AGATCGCTTT
    GAGAGACTGG TCGAGCTGGT CACCATGATG GGAGATCAGG GAGAGCTGCC CATAGCCATG
    GCTCTGGCCA ATGTGGTCCC ATGCTCTCAA TGGGATGAGC TGGCCAGAGT GTTAGTGACT
    CTCTTCGACT CTCGCCATCT GCTGTATCAG CTGTTGTGGA ATATGTTCTC CAAAGAGGTG
    GAGCTGGCAG ACTCAATGCA GACGCTCTTT AGAGGAAATA GCCTAGCCAG CAAGATTATG
    ACCTTCTGCT TCAAGGTGTA CGGAGCTGCG TATCTACAGA AGCTTCTGGA GCCGCTGTTG
    AGAGGAATCA TCACTACACC AGAGTGGCAG CAGATCAGTT TTGAAGTGGA CCCTACTAGA
    CTTGAACAGG CAGAGAACCT GGAGGAGAAC CAGAAGAACC TGCTTCAGAT CACTGACCGC
    TTCTTCCTGG CCATCATCAA CTCCTCGAGC GAGTTTCCTC CACAGCTGCG CAGTGTGTGC
    CACTGTCTGT ATCAGGTGGT CAGTCAGCGG TTTCCTCAGA ACAGCATTGG TGCTGTAGGA
    AGTGCCATGT TCCTGCGCTT TATTAACCCA GCTATCGTGT CCCCATATGA GGCTGGAATC
    CTAGACAAGA AACCCCCACC TCGCATCGAG CGTGGCCTTA AACTCATGTC TAAGATTCTG
    CAGAGCATTG CTAATCATGT TCTCTTCACA AAAGAGGAGC ATATGAGGCC CTTTAATGAT
    TTTGTCAAAA GCAACTTCGA TGCAGCCAGA AGGTTCTTCT TGGATATCGC ATCTGATTCT
    CCACCAAGTG ATTCTGTGAA CCATAGTCTG TCTTTCATCA GTGACGGTAA TGTGCTCGCT
    CTGCACCGGC TATTATGGAA CAACCAGGAA AGAATTGGCC AATACCTGTC CAGCAACAGG
    GACCACAAGG CAGTGGGCCG TAGACCATTT GACAAGATGG CCACTCTTCT GGCGTACCTG
    GGTCCTCCTG AACATAAGCC AGTAGCAGAC ACACACTGGT CCAGCCTCAA CCTCACCAGC
    TCAAAGTTTG AGGAGTTCAT GACAAGGCAT CAAGTCCATG AGAAGGAGGA ATTTAAAGCA
    TTAAAAACCC TCAACATATT TTACCAAGCT GGCACATCTA AGAACGGCAA CCCTGTGTTT
    TATTACATCG CACGCAGGTT CAAAACTGGA CAAATCAATG GTGATCTTCT GATCTACCAC
    GTCCTGCTGA CGCTCAAGCC CTACTATGCC AAGCATTATG AGATCGTAGT TGACCTTACT
    CATGTGGGCC CCAGTAACCG TTTCAAGACG GACTTCTTAT CCAAATGGTT TGTGGTCTTT
    CCAAACTTCG CCTATGAGAA CGTGGCTGCT GTGTACATCT ACAACTGCAA CACCTGGGTA
    CGCGAGTACA CCAAATACCA CGAGCGACTG CTGACTGGCC TCAAGGGGAG CAAGAAGCTG
    GTGTTTATCG ATTCGCCTGC ACGTCTAGCT GAACACATCG AACCCGAACA GCAGAAGCTG
    CCCGCAGCTA CTTTAGCACT GGAAGAAGAC CTCAAAGTGT TCCACAACGC CCTAAAGCTT
    GCACACAGAG ACACAAAAGT GTCAATTAAG GTGGGATCCA CAGCTGTACA GGTGACCTCG
    GCCGAAAGAA CGCGTGTCCT AGGACAGCCT GTGTTCCTAA ATGACATCTA CTACGCTTCA
    GAGATCGAGG AGATATGCCT GGTGGACGAG AACCAGTTCA CGCTAACCAT CGCTAACCAG
    GGCACCCCAC TCACTTTCAT GCACCAGGAG TGTGAAGCCA TCGTTCAGTC CATCATCCAC
    ATCCGCACGC GCTGGGAACT TTCCCAACCA GACTCCATTC CCCAACACAC CAAAATCAGG
    CCTAAGGATG TTCCTGGCAC CCTGCTCAAC ATCGCTCTTC TCAACCTGGG AAGCTCCGAC
    CCCAGTCTGA GGTCTGCGGC CTACAATCTC CTTTGTGCCT TAACCTGCAC CTTCAACCTG
    AAGATTGAAG GCCAGCTTCT GGAAACGTCT GGCTTGTGCA TCCCAGCCAA CAACACGCTT
    TTTATTGTGT CCATTAGCAA AACGCTAGCA GCTAATGAAC CCCACCTCAC TCTGGAGTTC
    CTGGAAGAGT GCATCTCAGG ATTCAGTAAA TCAAGTATAG AGCTGAAGCA TCTGTGTCTA
    GAATATATGA CTCCTTGGCT CCGTAATTTG GTGCGCTTTT GCAAACACAA TGATGACGCC
    AAACGGCAGC GTGTCACGGC CATCCTGGAC AAGCTTATTA CTATGACCAT CAACGAGAAG
    CAGATGTACC CCTCCATACA GGCCAAGATA TGGGGCAGCT TGGGTCAGAT CACAGACCTG
    CTTGATGTGG TGTTGGACAG CTTTATAAAG ACCAGCGCTA CTGGCGGTTT AGGATCCATC
    AAAGCTGAAG TGATGGCTGA CACTGCTGTA GCTTTGGCGT CAGGAAATGT CAAACTGGTC
    TCCAGCAAGG TGATTGGCCG AATGTGTAAG ATCATCGACA AGACGTGTCT GTCTCCCACT
    CCGACCCTTG AGCAGCACCT CATGTGGGAC GACATCGCCA TCCTGGCCCG GTACATGCTC
    ATGCTGTCCT TCAACAACTC TCTGGACGTG GCCGCTCATC TGCCATACCT GTTCCACGTC
    GTCACCCTGC TGGTGGCCAC GGGCCCTCTC TCGCTGCGTG CATCAACACA CGGCCTGGTC
    ATCAACATCA TTCACTCGCT CTGCACATGC TCACAACTCA ACTTCAGTGA GGAGACTAAA
    CAGGTGCTGC GCCTGAGTCT GACTGAGTTC TCCCTGCCCA AGTTCTACCT GCTGTTCGGC
    ATCAGTAAGG TCAAATCTGC GGCGGTCATC GCTTTCCGTT CCAGCTACAG AGACCGCTCA
    TTCTCTCCAG GCTCTTATGA GAGAGAGACA TTTGCTCTGT CTTCCCTGGA AACAGTCACG
    GAGGCTCTTT TGGAGATCAT GGAGGCCTGC ATGAGGGACA TTCCTGGATG CAAATGGCTA
    GATCAGTGGA CTGAATTGGC GCAAAAGTTT GCGTTCCAGT ATAACCCCTC TCTTCAGCCT
    CGGGCGCTGG TGGTGTTCGG CTGCATCAGT AAACGAGTGA CTCACGGACA GCTAAAGCAG
    ATCATTCGTA TTCTCAGTAA AGCCAGTCCT CTACTCAGCA TTGATCGGGG GCTGGAGAGC
    TGCTTAAAGG GCCCAGATAA TTACAACAGC CAGGTTCTCA TCGAAGCCAC AGTCATCGCT
    CTCACCAAAC TACAGCCTCT CCTCGATCAG GATTCTCCTA TGCATAAAGC TCTGTTCTGG
    GTGGCCGTGG CCGTGCTTCA GTTGGATGAA GTGAATCTGT ACTCCGCCGG CACTGCCTTA
    CTGGAGCAGA ACCTGCACAC ACTGGACAGC CTCCGAGTGT TTAATGATAA GAGTCCTGAA
    GAGGTTTTTA TGGAGATCCG GAAACCTCTA GAGTGGCACT GTAAACAAAT GGACCATTTT
    GTGGGACTCA ACTTTGCCTC CAACTTCAAT TTTGCATTAG TGGGCCATTT ACTTAAAGGA
    TACAGACATC CCTCTTCAAC AACAGTTGCC AGGACAGTTC GTATCCTGCA TACACTCCTG
    GGCCTGGTGG GAAAACACCG CAGCTGTGAC AAGTTTGAGG TGAACACGCA GAGTGTGGCC
    TATCTGGCAG CTTTGCTCAC AGTGTCAGAG GAAGTTAGGA GCCGCTGCAG TTTAAAGCAC
    AGGAAATCAT TGCTGCTTTC AGAAGTTACA ATGGAGAACT TCCCCATGGA CACATACACC
    GTTTACGACA CTGAACCCGG GTGCAGGCTA CTGCGTGAAA ACCAGCCTTG GGCTTCTCCG
    AAAGTGTCAG AGCGCAGTCT TGCACCTCCG TACTCCACAA TAGGGCAGAC CAGCCTTCGT
    CCACGCAAAT CCATGAGTGT AGACATGGGC CAGCCGTCTC AGGCCAATGC GAAGAAACTA
    CTGGGTATTA GCGGCACTAG AAAGAGTTTC GATCACTTAA TATCAGAATC CAAAGCCCCA
    AAAAGACCAG ACATGGAGTC GGGCATCACC ACACCTCCAA AGATGAGGCG GGTTTCTGAA
    AATGACTATG AGATCGAAAC TCAGCGAATA GCCAACTCTC ATTTACGCAA AGTGTCTGTG
    TCTGAATCCA ATGTCCTCCT GGACGAGAAC GTACTTACGG ACCCCAAGAT CCAGACGTTG
    CTCCTCACTG TACTGGCAAC ACTGGTGAAG TACACTACAG ACGAGTTTCA TCAGCGGATC
    TTGTATGAGT ATTTGGCTGA GGCCAGTGTG GTCTTCCCCA AGGTCTTTCC AGTTGTGCAC
    AACCTGCTGG ACTCCAAGTT AAGCACCGTC CTTTCCATGT GCCAGGACCC GAACCTTCTG
    AACCCCGTCC ATGGCATCAT TCAGAACGTG GTCTATCATG AGGAGTCTCC CCCTCAGTAC
    CAGCCGTCCT GCTTGCAGAG CTTTGGTTTC AATGGTCTTT GGAGGTTTGC CGGGCCGTTC
    TCTAAGCAAA CCCAAATTCC AGACTACGCC GAGCTGGTGG TTAAATTTTT GGATGCTTTG
    ATTGACACGT ATCTCCCGGG CATTGAGGAG GAAGGAGGAG AAGAGTCACT GCGGACCCCG
    ACCTCGCCAT ATCCACCAAC CGTCCAGAGC CAGCTGAGCA TCACAGCCAA TCTCAACCTG
    TCCAACTCCA TGACATCACT TGCCACATCA CAGCACTCCC CAGAGGGCCC TATAGTTTAT
    AGACGGTCCC CAACTCTCTC CCATCCACGA ACTACAGAGG AGGTGCCACC GAAAACAGCT
    GCCTGTCTGC CTTGCTCTAA GTCGGCTGTA TTCCCACCTC ACCTCGTCCT GCCAGGTACT
    CATCCACCTC TGCTGTGTGA AAGTAGCAGT TGTTGGCATT GGCCCGAGTG TTTGAATTGT
    AAATCTGCTG CTGTCAGATC AAAGAAAGCA AGAGGGGCTA CTATAACAGA TAGTGTGAAG
    AGAGAGCACA AGGGCCTGGT ATTAAGCAAA CACTTACATA CAGTACATAG CCAGGGGACA
    GGACATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55271
    Clone ID Related Accession (Same CDS sequence) XM_017351194.1
    Accession Version XM_017351194.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8406bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product neurofibromin isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGGCGGCGC ACAAGCCCGT GGAATGGGTC CAGGCCATCA TTAACAGATT CGACGAACAG 
    CTCCCAATCA AAACAGGACA GCAGAGCACC CACACCAAAG TGAGTACAGA GCACAACAAA
    GAATGTTTGA TCAACATCTC CAAGTACAAA TTTGCCCTCG TCATCAATGG CCTGACCAAC
    ATACTCAAAA ATGTGAACAA CATGCGGATA TTTGGCGAAG CTGCTGAGAA GAACCTCTAC
    TTCTCCCAGC TGATCATACT GGACACACTG GAGAAGTGTC TAGCAGGGCA ACCTAAGGAT
    TGCCTGAGGC TGGATGAGGC TATGTTGGTA AAACAGTTGC TGCCTGAAAT CTGTCACTTC
    CTGCACTCGT ACCGTGAGGG CCAGCAACAC GCCACTCAGC TGCGCAGCTC CGCCTCTGCT
    GTGCTTTTCT CTCTCAGCTG CAACAACTTT AATGCCGTCT TCAGTCGTAT CTCCACCAGG
    TTGCAAGAGC TCACAGTTTG TACAGAGGAC ACAGTGGATG TCCATGACAT TGAGCTCATT
    CAGTATATCA ATGTAGATTG CTCCAAGTTA AAGAGACTCC TACAAGAAGC AGTACTTAAG
    TTCAAGTCTT TGAAAAAGCC GGCTCAGCTT GCAGTAATCA GCAGTTTGGA AAAGGCTTTC
    TGGAACTGGG TTGAACACTA CCCTGATGAT TTCACTATGC TCTATCAGAG ACCCCAGGCC
    GACATGGCCG ACTGTGCAGA GAGACTGTTC GACCTAGTGG ACAGTTTTGC GGAAAGCGCA
    AAGAGAAAAG CTGCTGTGTG GCCACTGCAG ATCATCCTCC TCATCCTCTG TCCAGAGATC
    ACGCAGAGCA TCACCAAAGA AGTAGGGGGA GAGGAGAAAG CTAACAAGAA GTCATTTGTA
    GACAGTTTAC GAAAGGCTCT TGGCCAACAC AGCGGCAGCA AACAATTGAC GGAAAGTGCA
    GCTGTTGCAT GCGTCAAACT CTGCAAAGCC TGCACATATA TCAACTGGGA GGATAATTCT
    GTCATCTTTC TCCTGGTTCA GTCCATCGTC ATGGACCTTA AGGGTCTCCT GTTTAACCCC
    ACCAAACCTT TCTCAAGAGG TACGGGCTGC CAGAGCGCCG ATGTAGATTT GATGATCGAC
    TGCTTTGTGT CATGTTATCG CATCAATCCT CGCAACAACC AGCATTTTAA GGTGTGTCTG
    GCCTCGACTT CTCCTCCTAC CTTCCATTTC GTCCTTGTCA ACTCCCTGCA CAAAATTATC
    ACAAATTCTC ACCTGGACTG GTGGCCGAAG ATTGACGCCA TCTACTGCTA CTCAGGAGAA
    CTACGCACCA TGTTTGCAGA CACACTGGGT CGAGTGATTC AGAGTCTCAG TGCACATCCG
    CCTCTGAGGA TGACATCAAG TTTGACATTC AAAGAGAAAA TGACAACAAG TTTGAAGTTC
    AAGGAAAAGT CCACAGATCT TGATTCGCGT TCTTTGCTGC TGGCACTGGT CAAACTTATC
    CACGCTGACC CCAAACTAAT GCTGCACAAT CCGGGAAAAC CTGGTCAGGA GATTCAGAAC
    AGCACAGCTG AGATCATCAA AGGTCTGGTT CAGCTGGTTC CTCTTACCCA CAGTGCTGAG
    CTTTCCCAAG AGGCCATGGA GGCATTGCTT GTTTTACATC AGCCAGAAAC CATTGAGCTA
    TGGAACCCGG ACGCTCCCAT TGAGACCTTC TGGGACATCA GCTCTCAGGT GTTATTTCTA
    ATTTGTAAGA AGCTGATTGG ACACCAGATG ATCAACAGTA CAGAGATACT CAAGTGGCTC
    AGGGAGATTC TCATATGCAG GAACAAGTTT CTATTAAAGA ACAAGGAGTG TGCTACACAG
    GGCAGTCTGA TCCCCATCAG CAGACAGGCT CAGACCAAGC TGGAGGTGTG TCTGTACACG
    TTCCTGTGGA GTCCTGATGC AGAGGCTGTT TTGGTGGCCA TGTCCTGTTT CAGGCATCTC
    TGTGAGGAGG CCGATATTCG CTGCAGTGTC GATGAGCTGC CTGTTCACAC AGTCCTACCC
    AATTACAACA CCTTCATTGA GCTTGCATCC GTCAGCAATA TGATGGCCAC AGGTCGCAAC
    ACACTACAAA AAAGAGTGAT GGCACTTTTA AGAAGAATAA AGCACCCTAC AGCTGGAAAT
    GTAGAGGCAT GGGAAGACAC ATATGCAAAG TGGGAGCAAG CTACAAAACA GATACTCAAC
    CACCCAAAAA ACAAAGTGGA AGATGGACAG GTAGTTAACA TAAGATTGGT CAATTATTTC
    ATCTTTTTTT CCATTCATGG TCATCTATTT TCTGTTCGTC CTCAGGAGTG GATCAACATG
    ACTGGTTTCT TGTGCGCTCT GGGAGGTGTT TGTCTCCAAC AGCGCAGCAC GCCGAGTCTG
    GGCACCTACA GTCCTCCAAT GGGCCCCCTG ACCGAACGCA AGGGGTCCAT GATTTCCATG
    AGCTCCTGCG AGGTAAACAG CGAAACATCC CTGAATCGCT TTCTGGAGCG TCTGCTGGCT
    CTGATGGTCT GCACACATGA GAAAGTGGGA GTGCAAATTC GCACCAACGT CAAAGACCTG
    GTGGGACTGG AGCTCAGTCC GGCTCTCTAT TCAATGCTCT TCACTAAGCT GCGCAACAGC
    ATCGGACGCT TCTTTGACAC CCAGGGGCCG GTTCCTATTA CAGATGCAAA CACTCTGTTT
    GTAGAGCAGA CCATTGCTAT CATGAAGAAC TTGCTGGACA GTCACACTGA AGGAAGCTCA
    GAGCACCTTG GTCAAGCCAG CATTGAGACC ATGATGCTCA ATCTAGTTAG GTATGTGCGT
    ATTTTAGGGA ACATTGTTCA TGCCATTCAG ATCAAAACCA AGTTGTGTCA GTTAGTGGAG
    GTGATGATGG AGCGCAGGGA TGACCTCTCC TTCTGCCAGG AGATGAAATT CAGGAATAAA
    ATGGTGGAGT ACCTCACAGA CTGGGTGATG GGAACCTCCA ATCAAGCTGC GGACGATGAT
    GTCAAATGCC TGACCCGCGA TCTGGACCAG GCCAGTATGG AGGCTGTCGT GTCTCTGTTG
    GCTGGTCTTC CTCTACAGCC TGAGGAGGGT GACGGTGTTG AGCTCATGGA GGCCAAATCA
    CAGCTCTTCC TGAAGTACTT CACACTGTTC ATGAACCTGT TGAATGACTG CAGCGAGGTT
    GAGGAAGATG GCCAGCAGGT CGCAGGGAGG AAACGTGGCA TGTCTAGACG ACTGGCATCT
    CTGCGGCACT GCACAGTATT GGCTATGTCT AACTTGCTCA ATGCTAATGT GGATAGCGGC
    CTCATGCACT CAATCGGTCT GGGATACCAT AAGGACCTGC AGACACGGGC TACATTTATG
    GAAGTGCTCA CTAAGATCCT TCAGCAAGGG ACGGAGTTCG ACACGCTTGC AGAGACTGTG
    CTTGCAGATC GCTTTGAGAG ACTGGTCGAG CTGGTCACCA TGATGGGAGA TCAGGGAGAG
    CTGCCCATAG CCATGGCTCT GGCCAATGTG GTCCCATGCT CTCAATGGGA TGAGCTGGCC
    AGAGTGTTAG TGACTCTCTT CGACTCTCGC CATCTGCTGT ATCAGCTGTT GTGGAATATG
    TTCTCCAAAG AGGTGGAGCT GGCAGACTCA ATGCAGACGC TCTTTAGAGG AAATAGCCTA
    GCCAGCAAGA TTATGACCTT CTGCTTCAAG GTGTACGGAG CTGCGTATCT ACAGAAGCTT
    CTGGAGCCGC TGTTGAGAGG AATCATCACT ACACCAGAGT GGCAGCAGAT CAGTTTTGAA
    GTGGACCCTA CTAGACTTGA ACAGGCAGAG AACCTGGAGG AGAACCAGAA GAACCTGCTT
    CAGATCACTG ACCGCTTCTT CCTGGCCATC ATCAACTCCT CGAGCGAGTT TCCTCCACAG
    CTGCGCAGTG TGTGCCACTG TCTGTATCAG GTGGTCAGTC AGCGGTTTCC TCAGAACAGC
    ATTGGTGCTG TAGGAAGTGC CATGTTCCTG CGCTTTATTA ACCCAGCTAT CGTGTCCCCA
    TATGAGGCTG GAATCCTAGA CAAGAAACCC CCACCTCGCA TCGAGCGTGG CCTTAAACTC
    ATGTCTAAGA TTCTGCAGAG CATTGCTAAT CATGTTCTCT TCACAAAAGA GGAGCATATG
    AGGCCCTTTA ATGATTTTGT CAAAAGCAAC TTCGATGCAG CCAGAAGGTT CTTCTTGGAT
    ATCGCATCTG ATTCTCCACC AAGTGATTCT GTGAACCATA GTCTGTCTTT CATCAGTGAC
    GGTAATGTGC TCGCTCTGCA CCGGCTATTA TGGAACAACC AGGAAAGAAT TGGCCAATAC
    CTGTCCAGCA ACAGGGACCA CAAGGCAGTG GGCCGTAGAC CATTTGACAA GATGGCCACT
    CTTCTGGCGT ACCTGGGTCC TCCTGAACAT AAGCCAGTAG CAGACACACA CTGGTCCAGC
    CTCAACCTCA CCAGCTCAAA GTTTGAGGAG TTCATGACAA GGCATCAAGT CCATGAGAAG
    GAGGAATTTA AAGCATTAAA AACCCTCAAC ATATTTTACC AAGCTGGCAC ATCTAAGAAC
    GGCAACCCTG TGTTTTATTA CATCGCACGC AGGTTCAAAA CTGGACAAAT CAATGGTGAT
    CTTCTGATCT ACCACGTCCT GCTGACGCTC AAGCCCTACT ATGCCAAGCA TTATGAGATC
    GTAGTTGACC TTACTCATGT GGGCCCCAGT AACCGTTTCA AGACGGACTT CTTATCCAAA
    TGGTTTGTGG TCTTTCCAAA CTTCGCCTAT GAGAACGTGG CTGCTGTGTA CATCTACAAC
    TGCAACACCT GGGTACGCGA GTACACCAAA TACCACGAGC GACTGCTGAC TGGCCTCAAG
    GGGAGCAAGA AGCTGGTGTT TATCGATTCG CCTGCACGTC TAGCTGAACA CATCGAACCC
    GAACAGCAGA AGCTGCCCGC AGCTACTTTA GCACTGGAAG AAGACCTCAA AGTGTTCCAC
    AACGCCCTAA AGCTTGCACA CAGAGACACA AAAGTGTCAA TTAAGGTGGG ATCCACAGCT
    GTACAGGTGA CCTCGGCCGA AAGAACGCGT GTCCTAGGAC AGCCTGTGTT CCTAAATGAC
    ATCTACTACG CTTCAGAGAT CGAGGAGATA TGCCTGGTGG ACGAGAACCA GTTCACGCTA
    ACCATCGCTA ACCAGGGCAC CCCACTCACT TTCATGCACC AGGAGTGTGA AGCCATCGTT
    CAGTCCATCA TCCACATCCG CACGCGCTGG GAACTTTCCC AACCAGACTC CATTCCCCAA
    CACACCAAAA TCAGGCCTAA GGATGTTCCT GGCACCCTGC TCAACATCGC TCTTCTCAAC
    CTGGGAAGCT CCGACCCCAG TCTGAGGTCT GCGGCCTACA ATCTCCTTTG TGCCTTAACC
    TGCACCTTCA ACCTGAAGAT TGAAGGCCAG CTTCTGGAAA CGTCTGGCTT GTGCATCCCA
    GCCAACAACA CGCTTTTTAT TGTGTCCATT AGCAAAACGC TAGCAGCTAA TGAACCCCAC
    CTCACTCTGG AGTTCCTGGA AGAGTGCATC TCAGGATTCA GTAAATCAAG TATAGAGCTG
    AAGCATCTGT GTCTAGAATA TATGACTCCT TGGCTCCGTA ATTTGGTGCG CTTTTGCAAA
    CACAATGATG ACGCCAAACG GCAGCGTGTC ACGGCCATCC TGGACAAGCT TATTACTATG
    ACCATCAACG AGAAGCAGAT GTACCCCTCC ATACAGGCCA AGATATGGGG CAGCTTGGGT
    CAGATCACAG ACCTGCTTGA TGTGGTGTTG GACAGCTTTA TAAAGACCAG CGCTACTGGC
    GGTTTAGGAT CCATCAAAGC TGAAGTGATG GCTGACACTG CTGTAGCTTT GGCGTCAGGA
    AATGTCAAAC TGGTCTCCAG CAAGGTGATT GGCCGAATGT GTAAGATCAT CGACAAGACG
    TGTCTGTCTC CCACTCCGAC CCTTGAGCAG CACCTCATGT GGGACGACAT CGCCATCCTG
    GCCCGGTACA TGCTCATGCT GTCCTTCAAC AACTCTCTGG ACGTGGCCGC TCATCTGCCA
    TACCTGTTCC ACGTCGTCAC CCTGCTGGTG GCCACGGGCC CTCTCTCGCT GCGTGCATCA
    ACACACGGCC TGGTCATCAA CATCATTCAC TCGCTCTGCA CATGCTCACA ACTCAACTTC
    AGTGAGGAGA CTAAACAGGT GCTGCGCCTG AGTCTGACTG AGTTCTCCCT GCCCAAGTTC
    TACCTGCTGT TCGGCATCAG TAAGGTCAAA TCTGCGGCGG TCATCGCTTT CCGTTCCAGC
    TACAGAGACC GCTCATTCTC TCCAGGCTCT TATGAGAGAG AGACATTTGC TCTGTCTTCC
    CTGGAAACAG TCACGGAGGC TCTTTTGGAG ATCATGGAGG CCTGCATGAG GGACATTCCT
    GGATGCAAAT GGCTAGATCA GTGGACTGAA TTGGCGCAAA AGTTTGCGTT CCAGTATAAC
    CCCTCTCTTC AGCCTCGGGC GCTGGTGGTG TTCGGCTGCA TCAGTAAACG AGTGACTCAC
    GGACAGCTAA AGCAGATCAT TCGTATTCTC AGTAAAGCCA GTCCTCTACT CAGCATTGAT
    CGGGGGCTGG AGAGCTGCTT AAAGGGCCCA GATAATTACA ACAGCCAGGT TCTCATCGAA
    GCCACAGTCA TCGCTCTCAC CAAACTACAG CCTCTCCTCG ATCAGGATTC TCCTATGCAT
    AAAGCTCTGT TCTGGGTGGC CGTGGCCGTG CTTCAGTTGG ATGAAGTGAA TCTGTACTCC
    GCCGGCACTG CCTTACTGGA GCAGAACCTG CACACACTGG ACAGCCTCCG AGTGTTTAAT
    GATAAGAGTC CTGAAGAGGT TTTTATGGAG ATCCGGAAAC CTCTAGAGTG GCACTGTAAA
    CAAATGGACC ATTTTGTGGG ACTCAACTTT GCCTCCAACT TCAATTTTGC ATTAGTGGGC
    CATTTACTTA AAGGATACAG ACATCCCTCT TCAACAACAG TTGCCAGGAC AGTTCGTATC
    CTGCATACAC TCCTGGGCCT GGTGGGAAAA CACCGCAGCT GTGACAAGTT TGAGGTGAAC
    ACGCAGAGTG TGGCCTATCT GGCAGCTTTG CTCACAGTGT CAGAGGAAGT TAGGAGCCGC
    TGCAGTTTAA AGCACAGGAA ATCATTGCTG CTTTCAGAAG TTACAATGGA GAACTTCCCC
    ATGGACACAT ACACCGTTTA CGACACTGAA CCCGGGTGCA GGCTACTGCG TGAAAACCAG
    CCTTGGGCTT CTCCGAAAGT GTCAGAGCGC AGTCTTGCAC CTCCGTACTC CACAATAGGG
    CAGACCAGCC TTCGTCCACG CAAATCCATG AGTGTAGACA TGGGCCAGCC GTCTCAGGCC
    AATGCGAAGA AACTACTGGG TATTAGCGGC ACTAGAAAGA GTTTCGATCA CTTAATATCA
    GAATCCAAAG CCCCAAAAAG ACCAGACATG GAGTCGGGCA TCACCACACC TCCAAAGATG
    AGGCGGGTTT CTGAAAATGA CTATGAGATC GAAACTCAGC GAATAGCCAA CTCTCATTTA
    CGCAAAGTGT CTGTGTCTGA ATCCAATGTC CTCCTGGACG AGAACGTACT TACGGACCCC
    AAGATCCAGA CGTTGCTCCT CACTGTACTG GCAACACTGG TGAAGTACAC TACAGACGAG
    TTTCATCAGC GGATCTTGTA TGAGTATTTG GCTGAGGCCA GTGTGGTCTT CCCCAAGGTC
    TTTCCAGTTG TGCACAACCT GCTGGACTCC AAGTTAAGCA CCGTCCTTTC CATGTGCCAG
    GACCCGAACC TTCTGAACCC CGTCCATGGC ATCATTCAGA ACGTGGTCTA TCATGAGGAG
    TCTCCCCCTC AGTACCAGCC GTCCTGCTTG CAGAGCTTTG GTTTCAATGG TCTTTGGAGG
    TTTGCCGGGC CGTTCTCTAA GCAAACCCAA ATTCCAGACT ACGCCGAGCT GGTGGTTAAA
    TTTTTGGATG CTTTGATTGA CACGTATCTC CCGGGCATTG AGGAGGAAGG AGGAGAAGAG
    TCACTGCGGA CCCCGACCTC GCCATATCCA CCAACCGTCC AGAGCCAGCT GAGCATCACA
    GCCAATCTCA ACCTGTCCAA CTCCATGACA TCACTTGCCA CATCACAGCA CTCCCCAGCC
    TGTCTGCCTT GCTCTAAGTC GGCTGTATTC CCACCTCACC TCGTCCTGCC AGGTACTCAT
    CCACCTCTGC TGTGTGAAAG TAGCAGTTGT TGGCATTGGC CCGAGTGTTT GAATTGTAAA
    TCTGCTGCTG TCAGATCAAA GAAAGCAAGA GGGGCTACTA TAACAGATAG TGTGAAGAGA
    GAGCACAAGG GCCTGGTATT AAGCAAACAC TTACATACAG TACATAGCCA GGGGACAGGA
    CATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017206683.1
    CDS253..8658
    Translation

    Target ORF information:

    RefSeq Version XM_017351194.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio neurofibromin 1a (nf1a), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351194.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    ATGGCGGCGC ACAAGCCCGT GGAATGGGTC CAGGCCATCA TTAACAGATT CGACGAACAG 
    CTCCCAATCA AAACAGGACA GCAGAGCACC CACACCAAAG TGAGTACAGA GCACAACAAA
    GAATGTTTGA TCAACATCTC CAAGTACAAA TTTGCCCTCG TCATCAATGG CCTGACCAAC
    ATACTCAAAA ATGTGAACAA CATGCGGATA TTTGGCGAAG CTGCTGAGAA GAACCTCTAC
    TTCTCCCAGC TGATCATACT GGACACACTG GAGAAGTGTC TAGCAGGGCA ACCTAAGGAT
    TGCCTGAGGC TGGATGAGGC TATGTTGGTA AAACAGTTGC TGCCTGAAAT CTGTCACTTC
    CTGCACTCGT ACCGTGAGGG CCAGCAACAC GCCACTCAGC TGCGCAGCTC CGCCTCTGCT
    GTGCTTTTCT CTCTCAGCTG CAACAACTTT AATGCCGTCT TCAGTCGTAT CTCCACCAGG
    TTGCAAGAGC TCACAGTTTG TACAGAGGAC ACAGTGGATG TCCATGACAT TGAGCTCATT
    CAGTATATCA ATGTAGATTG CTCCAAGTTA AAGAGACTCC TACAAGAAGC AGTACTTAAG
    TTCAAGTCTT TGAAAAAGCC GGCTCAGCTT GCAGTAATCA GCAGTTTGGA AAAGGCTTTC
    TGGAACTGGG TTGAACACTA CCCTGATGAT TTCACTATGC TCTATCAGAG ACCCCAGGCC
    GACATGGCCG ACTGTGCAGA GAGACTGTTC GACCTAGTGG ACAGTTTTGC GGAAAGCGCA
    AAGAGAAAAG CTGCTGTGTG GCCACTGCAG ATCATCCTCC TCATCCTCTG TCCAGAGATC
    ACGCAGAGCA TCACCAAAGA AGTAGGGGGA GAGGAGAAAG CTAACAAGAA GTCATTTGTA
    GACAGTTTAC GAAAGGCTCT TGGCCAACAC AGCGGCAGCA AACAATTGAC GGAAAGTGCA
    GCTGTTGCAT GCGTCAAACT CTGCAAAGCC TGCACATATA TCAACTGGGA GGATAATTCT
    GTCATCTTTC TCCTGGTTCA GTCCATCGTC ATGGACCTTA AGGGTCTCCT GTTTAACCCC
    ACCAAACCTT TCTCAAGAGG TACGGGCTGC CAGAGCGCCG ATGTAGATTT GATGATCGAC
    TGCTTTGTGT CATGTTATCG CATCAATCCT CGCAACAACC AGCATTTTAA GGTGTGTCTG
    GCCTCGACTT CTCCTCCTAC CTTCCATTTC GTCCTTGTCA ACTCCCTGCA CAAAATTATC
    ACAAATTCTC ACCTGGACTG GTGGCCGAAG ATTGACGCCA TCTACTGCTA CTCAGGAGAA
    CTACGCACCA TGTTTGCAGA CACACTGGGT CGAGTGATTC AGAGTCTCAG TGCACATCCG
    CCTCTGAGGA TGACATCAAG TTTGACATTC AAAGAGAAAA TGACAACAAG TTTGAAGTTC
    AAGGAAAAGT CCACAGATCT TGATTCGCGT TCTTTGCTGC TGGCACTGGT CAAACTTATC
    CACGCTGACC CCAAACTAAT GCTGCACAAT CCGGGAAAAC CTGGTCAGGA GATTCAGAAC
    AGCACAGCTG AGATCATCAA AGGTCTGGTT CAGCTGGTTC CTCTTACCCA CAGTGCTGAG
    CTTTCCCAAG AGGCCATGGA GGCATTGCTT GTTTTACATC AGCCAGAAAC CATTGAGCTA
    TGGAACCCGG ACGCTCCCAT TGAGACCTTC TGGGACATCA GCTCTCAGGT GTTATTTCTA
    ATTTGTAAGA AGCTGATTGG ACACCAGATG ATCAACAGTA CAGAGATACT CAAGTGGCTC
    AGGGAGATTC TCATATGCAG GAACAAGTTT CTATTAAAGA ACAAGGAGTG TGCTACACAG
    GGCAGTCTGA TCCCCATCAG CAGACAGGCT CAGACCAAGC TGGAGGTGTG TCTGTACACG
    TTCCTGTGGA GTCCTGATGC AGAGGCTGTT TTGGTGGCCA TGTCCTGTTT CAGGCATCTC
    TGTGAGGAGG CCGATATTCG CTGCAGTGTC GATGAGCTGC CTGTTCACAC AGTCCTACCC
    AATTACAACA CCTTCATTGA GCTTGCATCC GTCAGCAATA TGATGGCCAC AGGTCGCAAC
    ACACTACAAA AAAGAGTGAT GGCACTTTTA AGAAGAATAA AGCACCCTAC AGCTGGAAAT
    GTAGAGGCAT GGGAAGACAC ATATGCAAAG TGGGAGCAAG CTACAAAACA GATACTCAAC
    CACCCAAAAA ACAAAGTGGA AGATGGACAG GTAGTTAACA TAAGATTGGT CAATTATTTC
    ATCTTTTTTT CCATTCATGG TCATCTATTT TCTGTTCGTC CTCAGGAGTG GATCAACATG
    ACTGGTTTCT TGTGCGCTCT GGGAGGTGTT TGTCTCCAAC AGCGCAGCAC GCCGAGTCTG
    GGCACCTACA GTCCTCCAAT GGGCCCCCTG ACCGAACGCA AGGGGTCCAT GATTTCCATG
    AGCTCCTGCG AGGTAAACAG CGAAACATCC CTGAATCGCT TTCTGGAGCG TCTGCTGGCT
    CTGATGGTCT GCACACATGA GAAAGTGGGA GTGCAAATTC GCACCAACGT CAAAGACCTG
    GTGGGACTGG AGCTCAGTCC GGCTCTCTAT TCAATGCTCT TCACTAAGCT GCGCAACAGC
    ATCGGACGCT TCTTTGACAC CCAGGGGCCG GTTCCTATTA CAGATGCAAA CACTCTGTTT
    GTAGAGCAGA CCATTGCTAT CATGAAGAAC TTGCTGGACA GTCACACTGA AGGAAGCTCA
    GAGCACCTTG GTCAAGCCAG CATTGAGACC ATGATGCTCA ATCTAGTTAG GTATGTGCGT
    ATTTTAGGGA ACATTGTTCA TGCCATTCAG ATCAAAACCA AGTTGTGTCA GTTAGTGGAG
    GTGATGATGG AGCGCAGGGA TGACCTCTCC TTCTGCCAGG AGATGAAATT CAGGAATAAA
    ATGGTGGAGT ACCTCACAGA CTGGGTGATG GGAACCTCCA ATCAAGCTGC GGACGATGAT
    GTCAAATGCC TGACCCGCGA TCTGGACCAG GCCAGTATGG AGGCTGTCGT GTCTCTGTTG
    GCTGGTCTTC CTCTACAGCC TGAGGAGGGT GACGGTGTTG AGCTCATGGA GGCCAAATCA
    CAGCTCTTCC TGAAGTACTT CACACTGTTC ATGAACCTGT TGAATGACTG CAGCGAGGTT
    GAGGAAGATG GCCAGCAGGT CGCAGGGAGG AAACGTGGCA TGTCTAGACG ACTGGCATCT
    CTGCGGCACT GCACAGTATT GGCTATGTCT AACTTGCTCA ATGCTAATGT GGATAGCGGC
    CTCATGCACT CAATCGGTCT GGGATACCAT AAGGACCTGC AGACACGGGC TACATTTATG
    GAAGTGCTCA CTAAGATCCT TCAGCAAGGG ACGGAGTTCG ACACGCTTGC AGAGACTGTG
    CTTGCAGATC GCTTTGAGAG ACTGGTCGAG CTGGTCACCA TGATGGGAGA TCAGGGAGAG
    CTGCCCATAG CCATGGCTCT GGCCAATGTG GTCCCATGCT CTCAATGGGA TGAGCTGGCC
    AGAGTGTTAG TGACTCTCTT CGACTCTCGC CATCTGCTGT ATCAGCTGTT GTGGAATATG
    TTCTCCAAAG AGGTGGAGCT GGCAGACTCA ATGCAGACGC TCTTTAGAGG AAATAGCCTA
    GCCAGCAAGA TTATGACCTT CTGCTTCAAG GTGTACGGAG CTGCGTATCT ACAGAAGCTT
    CTGGAGCCGC TGTTGAGAGG AATCATCACT ACACCAGAGT GGCAGCAGAT CAGTTTTGAA
    GTGGACCCTA CTAGACTTGA ACAGGCAGAG AACCTGGAGG AGAACCAGAA GAACCTGCTT
    CAGATCACTG ACCGCTTCTT CCTGGCCATC ATCAACTCCT CGAGCGAGTT TCCTCCACAG
    CTGCGCAGTG TGTGCCACTG TCTGTATCAG GTGGTCAGTC AGCGGTTTCC TCAGAACAGC
    ATTGGTGCTG TAGGAAGTGC CATGTTCCTG CGCTTTATTA ACCCAGCTAT CGTGTCCCCA
    TATGAGGCTG GAATCCTAGA CAAGAAACCC CCACCTCGCA TCGAGCGTGG CCTTAAACTC
    ATGTCTAAGA TTCTGCAGAG CATTGCTAAT CATGTTCTCT TCACAAAAGA GGAGCATATG
    AGGCCCTTTA ATGATTTTGT CAAAAGCAAC TTCGATGCAG CCAGAAGGTT CTTCTTGGAT
    ATCGCATCTG ATTCTCCACC AAGTGATTCT GTGAACCATA GTCTGTCTTT CATCAGTGAC
    GGTAATGTGC TCGCTCTGCA CCGGCTATTA TGGAACAACC AGGAAAGAAT TGGCCAATAC
    CTGTCCAGCA ACAGGGACCA CAAGGCAGTG GGCCGTAGAC CATTTGACAA GATGGCCACT
    CTTCTGGCGT ACCTGGGTCC TCCTGAACAT AAGCCAGTAG CAGACACACA CTGGTCCAGC
    CTCAACCTCA CCAGCTCAAA GTTTGAGGAG TTCATGACAA GGCATCAAGT CCATGAGAAG
    GAGGAATTTA AAGCATTAAA AACCCTCAAC ATATTTTACC AAGCTGGCAC ATCTAAGAAC
    GGCAACCCTG TGTTTTATTA CATCGCACGC AGGTTCAAAA CTGGACAAAT CAATGGTGAT
    CTTCTGATCT ACCACGTCCT GCTGACGCTC AAGCCCTACT ATGCCAAGCA TTATGAGATC
    GTAGTTGACC TTACTCATGT GGGCCCCAGT AACCGTTTCA AGACGGACTT CTTATCCAAA
    TGGTTTGTGG TCTTTCCAAA CTTCGCCTAT GAGAACGTGG CTGCTGTGTA CATCTACAAC
    TGCAACACCT GGGTACGCGA GTACACCAAA TACCACGAGC GACTGCTGAC TGGCCTCAAG
    GGGAGCAAGA AGCTGGTGTT TATCGATTCG CCTGCACGTC TAGCTGAACA CATCGAACCC
    GAACAGCAGA AGCTGCCCGC AGCTACTTTA GCACTGGAAG AAGACCTCAA AGTGTTCCAC
    AACGCCCTAA AGCTTGCACA CAGAGACACA AAAGTGTCAA TTAAGGTGGG ATCCACAGCT
    GTACAGGTGA CCTCGGCCGA AAGAACGCGT GTCCTAGGAC AGCCTGTGTT CCTAAATGAC
    ATCTACTACG CTTCAGAGAT CGAGGAGATA TGCCTGGTGG ACGAGAACCA GTTCACGCTA
    ACCATCGCTA ACCAGGGCAC CCCACTCACT TTCATGCACC AGGAGTGTGA AGCCATCGTT
    CAGTCCATCA TCCACATCCG CACGCGCTGG GAACTTTCCC AACCAGACTC CATTCCCCAA
    CACACCAAAA TCAGGCCTAA GGATGTTCCT GGCACCCTGC TCAACATCGC TCTTCTCAAC
    CTGGGAAGCT CCGACCCCAG TCTGAGGTCT GCGGCCTACA ATCTCCTTTG TGCCTTAACC
    TGCACCTTCA ACCTGAAGAT TGAAGGCCAG CTTCTGGAAA CGTCTGGCTT GTGCATCCCA
    GCCAACAACA CGCTTTTTAT TGTGTCCATT AGCAAAACGC TAGCAGCTAA TGAACCCCAC
    CTCACTCTGG AGTTCCTGGA AGAGTGCATC TCAGGATTCA GTAAATCAAG TATAGAGCTG
    AAGCATCTGT GTCTAGAATA TATGACTCCT TGGCTCCGTA ATTTGGTGCG CTTTTGCAAA
    CACAATGATG ACGCCAAACG GCAGCGTGTC ACGGCCATCC TGGACAAGCT TATTACTATG
    ACCATCAACG AGAAGCAGAT GTACCCCTCC ATACAGGCCA AGATATGGGG CAGCTTGGGT
    CAGATCACAG ACCTGCTTGA TGTGGTGTTG GACAGCTTTA TAAAGACCAG CGCTACTGGC
    GGTTTAGGAT CCATCAAAGC TGAAGTGATG GCTGACACTG CTGTAGCTTT GGCGTCAGGA
    AATGTCAAAC TGGTCTCCAG CAAGGTGATT GGCCGAATGT GTAAGATCAT CGACAAGACG
    TGTCTGTCTC CCACTCCGAC CCTTGAGCAG CACCTCATGT GGGACGACAT CGCCATCCTG
    GCCCGGTACA TGCTCATGCT GTCCTTCAAC AACTCTCTGG ACGTGGCCGC TCATCTGCCA
    TACCTGTTCC ACGTCGTCAC CCTGCTGGTG GCCACGGGCC CTCTCTCGCT GCGTGCATCA
    ACACACGGCC TGGTCATCAA CATCATTCAC TCGCTCTGCA CATGCTCACA ACTCAACTTC
    AGTGAGGAGA CTAAACAGGT GCTGCGCCTG AGTCTGACTG AGTTCTCCCT GCCCAAGTTC
    TACCTGCTGT TCGGCATCAG TAAGGTCAAA TCTGCGGCGG TCATCGCTTT CCGTTCCAGC
    TACAGAGACC GCTCATTCTC TCCAGGCTCT TATGAGAGAG AGACATTTGC TCTGTCTTCC
    CTGGAAACAG TCACGGAGGC TCTTTTGGAG ATCATGGAGG CCTGCATGAG GGACATTCCT
    GGATGCAAAT GGCTAGATCA GTGGACTGAA TTGGCGCAAA AGTTTGCGTT CCAGTATAAC
    CCCTCTCTTC AGCCTCGGGC GCTGGTGGTG TTCGGCTGCA TCAGTAAACG AGTGACTCAC
    GGACAGCTAA AGCAGATCAT TCGTATTCTC AGTAAAGCCA GTCCTCTACT CAGCATTGAT
    CGGGGGCTGG AGAGCTGCTT AAAGGGCCCA GATAATTACA ACAGCCAGGT TCTCATCGAA
    GCCACAGTCA TCGCTCTCAC CAAACTACAG CCTCTCCTCG ATCAGGATTC TCCTATGCAT
    AAAGCTCTGT TCTGGGTGGC CGTGGCCGTG CTTCAGTTGG ATGAAGTGAA TCTGTACTCC
    GCCGGCACTG CCTTACTGGA GCAGAACCTG CACACACTGG ACAGCCTCCG AGTGTTTAAT
    GATAAGAGTC CTGAAGAGGT TTTTATGGAG ATCCGGAAAC CTCTAGAGTG GCACTGTAAA
    CAAATGGACC ATTTTGTGGG ACTCAACTTT GCCTCCAACT TCAATTTTGC ATTAGTGGGC
    CATTTACTTA AAGGATACAG ACATCCCTCT TCAACAACAG TTGCCAGGAC AGTTCGTATC
    CTGCATACAC TCCTGGGCCT GGTGGGAAAA CACCGCAGCT GTGACAAGTT TGAGGTGAAC
    ACGCAGAGTG TGGCCTATCT GGCAGCTTTG CTCACAGTGT CAGAGGAAGT TAGGAGCCGC
    TGCAGTTTAA AGCACAGGAA ATCATTGCTG CTTTCAGAAG TTACAATGGA GAACTTCCCC
    ATGGACACAT ACACCGTTTA CGACACTGAA CCCGGGTGCA GGCTACTGCG TGAAAACCAG
    CCTTGGGCTT CTCCGAAAGT GTCAGAGCGC AGTCTTGCAC CTCCGTACTC CACAATAGGG
    CAGACCAGCC TTCGTCCACG CAAATCCATG AGTGTAGACA TGGGCCAGCC GTCTCAGGCC
    AATGCGAAGA AACTACTGGG TATTAGCGGC ACTAGAAAGA GTTTCGATCA CTTAATATCA
    GAATCCAAAG CCCCAAAAAG ACCAGACATG GAGTCGGGCA TCACCACACC TCCAAAGATG
    AGGCGGGTTT CTGAAAATGA CTATGAGATC GAAACTCAGC GAATAGCCAA CTCTCATTTA
    CGCAAAGTGT CTGTGTCTGA ATCCAATGTC CTCCTGGACG AGAACGTACT TACGGACCCC
    AAGATCCAGA CGTTGCTCCT CACTGTACTG GCAACACTGG TGAAGTACAC TACAGACGAG
    TTTCATCAGC GGATCTTGTA TGAGTATTTG GCTGAGGCCA GTGTGGTCTT CCCCAAGGTC
    TTTCCAGTTG TGCACAACCT GCTGGACTCC AAGTTAAGCA CCGTCCTTTC CATGTGCCAG
    GACCCGAACC TTCTGAACCC CGTCCATGGC ATCATTCAGA ACGTGGTCTA TCATGAGGAG
    TCTCCCCCTC AGTACCAGCC GTCCTGCTTG CAGAGCTTTG GTTTCAATGG TCTTTGGAGG
    TTTGCCGGGC CGTTCTCTAA GCAAACCCAA ATTCCAGACT ACGCCGAGCT GGTGGTTAAA
    TTTTTGGATG CTTTGATTGA CACGTATCTC CCGGGCATTG AGGAGGAAGG AGGAGAAGAG
    TCACTGCGGA CCCCGACCTC GCCATATCCA CCAACCGTCC AGAGCCAGCT GAGCATCACA
    GCCAATCTCA ACCTGTCCAA CTCCATGACA TCACTTGCCA CATCACAGCA CTCCCCAGCC
    TGTCTGCCTT GCTCTAAGTC GGCTGTATTC CCACCTCACC TCGTCCTGCC AGGTACTCAT
    CCACCTCTGC TGTGTGAAAG TAGCAGTTGT TGGCATTGGC CCGAGTGTTT GAATTGTAAA
    TCTGCTGCTG TCAGATCAAA GAAAGCAAGA GGGGCTACTA TAACAGATAG TGTGAAGAGA
    GAGCACAAGG GCCTGGTATT AAGCAAACAC TTACATACAG TACATAGCCA GGGGACAGGA
    CATTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55272
    Clone ID Related Accession (Same CDS sequence) XM_017351195.1
    Accession Version XM_017351195.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8355bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product neurofibromin isoform X6
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCGGCGC ACAAGCCCGT GGAATGGGTC CAGGCCATCA TTAACAGATT CGACGAACAG 
    CTCCCAATCA AAACAGGACA GCAGAGCACC CACACCAAAG TGAGTACAGA GCACAACAAA
    GAATGTTTGA TCAACATCTC CAAGTACAAA TTTGCCCTCG TCATCAATGG CCTGACCAAC
    ATACTCAAAA ATGTGAACAA CATGCGGATA TTTGGCGAAG CTGCTGAGAA GAACCTCTAC
    TTCTCCCAGC TGATCATACT GGACACACTG GAGAAGTGTC TAGCAGGGCA ACCTAAGGAT
    TGCCTGAGGC TGGATGAGGC TATGTTGGTA AAACAGTTGC TGCCTGAAAT CTGTCACTTC
    CTGCACTCGT ACCGTGAGGG CCAGCAACAC GCCACTCAGC TGCGCAGCTC CGCCTCTGCT
    GTGCTTTTCT CTCTCAGCTG CAACAACTTT AATGCCGTCT TCAGTCGTAT CTCCACCAGG
    TTGCAAGAGC TCACAGTTTG TACAGAGGAC ACAGTGGATG TCCATGACAT TGAGCTCATT
    CAGTATATCA ATGTAGATTG CTCCAAGTTA AAGAGACTCC TACAAGAAGC AGTACTTAAG
    TTCAAGTCTT TGAAAAAGCC GGCTCAGCTT GCAGTAATCA GCAGTTTGGA AAAGGCTTTC
    TGGAACTGGG TTGAACACTA CCCTGATGAT TTCACTATGC TCTATCAGAG ACCCCAGGCC
    GACATGGCCG ACTGTGCAGA GAGACTGTTC GACCTAGTGG ACAGTTTTGC GGAAAGCGCA
    AAGAGAAAAG CTGCTGTGTG GCCACTGCAG ATCATCCTCC TCATCCTCTG TCCAGAGATC
    ACGCAGAGCA TCACCAAAGA AGTAGGGGGA GAGGAGAAAG CTAACAAGAA GTCATTTGTA
    GACAGTTTAC GAAAGGCTCT TGGCCAACAC AGCGGCAGCA AACAATTGAC GGAAAGTGCA
    GCTGTTGCAT GCGTCAAACT CTGCAAAGCC TGCACATATA TCAACTGGGA GGATAATTCT
    GTCATCTTTC TCCTGGTTCA GTCCATCGTC ATGGACCTTA AGGGTCTCCT GTTTAACCCC
    ACCAAACCTT TCTCAAGAGG TACGGGCTGC CAGAGCGCCG ATGTAGATTT GATGATCGAC
    TGCTTTGTGT CATGTTATCG CATCAATCCT CGCAACAACC AGCATTTTAA GGTGTGTCTG
    GCCTCGACTT CTCCTCCTAC CTTCCATTTC GTCCTTGTCA ACTCCCTGCA CAAAATTATC
    ACAAATTCTC ACCTGGACTG GTGGCCGAAG ATTGACGCCA TCTACTGCTA CTCAGGAGAA
    CTACGCACCA TGTTTGCAGA CACACTGGGT CGAGTGATTC AGAGTCTCAG TGCACATCCG
    CCTCTGAGGA TGACATCAAG TTTGACATTC AAAGAGAAAA TGACAACAAG TTTGAAGTTC
    AAGGAAAAGT CCACAGATCT TGATTCGCGT TCTTTGCTGC TGGCACTGGT CAAACTTATC
    CACGCTGACC CCAAACTAAT GCTGCACAAT CCGGGAAAAC CTGGTCAGGA GATTCAGAAC
    AGCACAGCTG AGATCATCAA AGGTCTGGTT CAGCTGGTTC CTCTTACCCA CAGTGCTGAG
    CTTTCCCAAG AGGCCATGGA GGCATTGCTT GTTTTACATC AGCCAGAAAC CATTGAGCTA
    TGGAACCCGG ACGCTCCCAT TGAGACCTTC TGGGACATCA GCTCTCAGGT GTTATTTCTA
    ATTTGTAAGA AGCTGATTGG ACACCAGATG ATCAACAGTA CAGAGATACT CAAGTGGCTC
    AGGGAGATTC TCATATGCAG GAACAAGTTT CTATTAAAGA ACAAGGAGTG TGCTACACAG
    GGCAGTCTGA TCCCCATCAG CAGACAGGCT CAGACCAAGC TGGAGGTGTG TCTGTACACG
    TTCCTGTGGA GTCCTGATGC AGAGGCTGTT TTGGTGGCCA TGTCCTGTTT CAGGCATCTC
    TGTGAGGAGG CCGATATTCG CTGCAGTGTC GATGAGCTGC CTGTTCACAC AGTCCTACCC
    AATTACAACA CCTTCATTGA GCTTGCATCC GTCAGCAATA TGATGGCCAC AGGTCGCAAC
    ACACTACAAA AAAGAGTGAT GGCACTTTTA AGAAGAATAA AGCACCCTAC AGCTGGAAAT
    GTAGAGGCAT GGGAAGACAC ATATGCAAAG TGGGAGCAAG CTACAAAACA GATACTCAAC
    CACCCAAAAA ACAAAGTGGA AGATGGACAG GTAGTTAACA TAAGATTGGT CAATTATTTC
    ATCTTTTTTT CCATTCATGG TCATCTATTT TCTGTTCGTC CTCAGGAGTG GATCAACATG
    ACTGGTTTCT TGTGCGCTCT GGGAGGTGTT TGTCTCCAAC AGCGCAGCAC GCCGAGTCTG
    GGCACCTACA GTCCTCCAAT GGGCCCCCTG ACCGAACGCA AGGGGTCCAT GATTTCCATG
    AGCTCCTGCG AGGTAAACAG CGAAACATCC CTGAATCGCT TTCTGGAGCG TCTGCTGGCT
    CTGATGGTCT GCACACATGA GAAAGTGGGA GTGCAAATTC GCACCAACGT CAAAGACCTG
    GTGGGACTGG AGCTCAGTCC GGCTCTCTAT TCAATGCTCT TCACTAAGCT GCGCAACAGC
    ATCGGACGCT TCTTTGACAC CCAGGGGCCG GTTCCTATTA CAGATGCAAA CACTCTGTTT
    GTAGAGCAGA CCATTGCTAT CATGAAGAAC TTGCTGGACA GTCACACTGA AGGAAGCTCA
    GAGCACCTTG GTCAAGCCAG CATTGAGACC ATGATGCTCA ATCTAGTTAG GTATGTGCGT
    ATTTTAGGGA ACATTGTTCA TGCCATTCAG ATCAAAACCA AGTTGTGTCA GTTAGTGGAG
    GTGATGATGG AGCGCAGGGA TGACCTCTCC TTCTGCCAGG AGATGAAATT CAGGAATAAA
    ATGGTGGAGT ACCTCACAGA CTGGGTGATG GGAACCTCCA ATCAAGCTGC GGACGATGAT
    GTCAAATGCC TGACCCGCGA TCTGGACCAG GCCAGTATGG AGGCTGTCGT GTCTCTGTTG
    GCTGGTCTTC CTCTACAGCC TGAGGAGGGT GACGGTGTTG AGCTCATGGA GGCCAAATCA
    CAGCTCTTCC TGAAGTACTT CACACTGTTC ATGAACCTGT TGAATGACTG CAGCGAGGTT
    GAGGAAGATG GCCAGCAGGT CGCAGGGAGG AAACGTGGCA TGTCTAGACG ACTGGCATCT
    CTGCGGCACT GCACAGTATT GGCTATGTCT AACTTGCTCA ATGCTAATGT GGATAGCGGC
    CTCATGCACT CAATCGGTCT GGGATACCAT AAGGACCTGC AGACACGGGC TACATTTATG
    GAAGTGCTCA CTAAGATCCT TCAGCAAGGG ACGGAGTTCG ACACGCTTGC AGAGACTGTG
    CTTGCAGATC GCTTTGAGAG ACTGGTCGAG CTGGTCACCA TGATGGGAGA TCAGGGAGAG
    CTGCCCATAG CCATGGCTCT GGCCAATGTG GTCCCATGCT CTCAATGGGA TGAGCTGGCC
    AGAGTGTTAG TGACTCTCTT CGACTCTCGC CATCTGCTGT ATCAGCTGTT GTGGAATATG
    TTCTCCAAAG AGGTGGAGCT GGCAGACTCA ATGCAGACGC TCTTTAGAGG AAATAGCCTA
    GCCAGCAAGA TTATGACCTT CTGCTTCAAG GTGTACGGAG CTGCGTATCT ACAGAAGCTT
    CTGGAGCCGC TGTTGAGAGG AATCATCACT ACACCAGAGT GGCAGCAGAT CAGTTTTGAA
    GTGGACCCTA CTAGACTTGA ACAGGCAGAG AACCTGGAGG AGAACCAGAA GAACCTGCTT
    CAGATCACTG ACCGCTTCTT CCTGGCCATC ATCAACTCCT CGAGCGAGTT TCCTCCACAG
    CTGCGCAGTG TGTGCCACTG TCTGTATCAG GTGGTCAGTC AGCGGTTTCC TCAGAACAGC
    ATTGGTGCTG TAGGAAGTGC CATGTTCCTG CGCTTTATTA ACCCAGCTAT CGTGTCCCCA
    TATGAGGCTG GAATCCTAGA CAAGAAACCC CCACCTCGCA TCGAGCGTGG CCTTAAACTC
    ATGTCTAAGA TTCTGCAGAG CATTGCTAAT CATGTTCTCT TCACAAAAGA GGAGCATATG
    AGGCCCTTTA ATGATTTTGT CAAAAGCAAC TTCGATGCAG CCAGAAGGTT CTTCTTGGAT
    ATCGCATCTG ATTCTCCACC AAGTGATTCT GTGAACCATA GTCTGTCTTT CATCAGTGAC
    GGTAATGTGC TCGCTCTGCA CCGGCTATTA TGGAACAACC AGGAAAGAAT TGGCCAATAC
    CTGTCCAGCA ACAGGGACCA CAAGGCAGTG GGCCGTAGAC CATTTGACAA GATGGCCACT
    CTTCTGGCGT ACCTGGGTCC TCCTGAACAT AAGCCAGTAG CAGACACACA CTGGTCCAGC
    CTCAACCTCA CCAGCTCAAA GTTTGAGGAG TTCATGACAA GGCATCAAGT CCATGAGAAG
    GAGGAATTTA AAGCATTAAA AACCCTCAAC ATATTTTACC AAGCTGGCAC ATCTAAGAAC
    GGCAACCCTG TGTTTTATTA CATCGCACGC AGGTTCAAAA CTGGACAAAT CAATGGTGAT
    CTTCTGATCT ACCACGTCCT GCTGACGCTC AAGCCCTACT ATGCCAAGCA TTATGAGATC
    GTAGTTGACC TTACTCATGT GGGCCCCAGT AACCGTTTCA AGACGGACTT CTTATCCAAA
    TGGTTTGTGG TCTTTCCAAA CTTCGCCTAT GAGAACGTGG CTGCTGTGTA CATCTACAAC
    TGCAACACCT GGGTACGCGA GTACACCAAA TACCACGAGC GACTGCTGAC TGGCCTCAAG
    GGGAGCAAGA AGCTGGTGTT TATCGATTCG CCTGCACGTC TAGCTGAACA CATCGAACCC
    GAACAGCAGA AGCTGCCCGC AGCTACTTTA GCACTGGAAG AAGACCTCAA AGTGTTCCAC
    AACGCCCTAA AGCTTGCACA CAGAGACACA AAAGTGTCAA TTAAGGTGGG ATCCACAGCT
    GTACAGGTGA CCTCGGCCGA AAGAACGCGT GTCCTAGGAC AGCCTGTGTT CCTAAATGAC
    ATCTACTACG CTTCAGAGAT CGAGGAGATA TGCCTGGTGG ACGAGAACCA GTTCACGCTA
    ACCATCGCTA ACCAGGGCAC CCCACTCACT TTCATGCACC AGGAGTGTGA AGCCATCGTT
    CAGTCCATCA TCCACATCCG CACGCGCTGG GAACTTTCCC AACCAGACTC CATTCCCCAA
    CACACCAAAA TCAGGCCTAA GGATGTTCCT GGCACCCTGC TCAACATCGC TCTTCTCAAC
    CTGGGAAGCT CCGACCCCAG TCTGAGGTCT GCGGCCTACA ATCTCCTTTG TGCCTTAACC
    TGCACCTTCA ACCTGAAGAT TGAAGGCCAG CTTCTGGAAA CGTCTGGCTT GTGCATCCCA
    GCCAACAACA CGCTTTTTAT TGTGTCCATT AGCAAAACGC TAGCAGCTAA TGAACCCCAC
    CTCACTCTGG AGTTCCTGGA AGAGTGCATC TCAGGATTCA GTAAATCAAG TATAGAGCTG
    AAGCATCTGT GTCTAGAATA TATGACTCCT TGGCTCCGTA ATTTGGTGCG CTTTTGCAAA
    CACAATGATG ACGCCAAACG GCAGCGTGTC ACGGCCATCC TGGACAAGCT TATTACTATG
    ACCATCAACG AGAAGCAGAT GTACCCCTCC ATACAGGCCA AGATATGGGG CAGCTTGGGT
    CAGATCACAG ACCTGCTTGA TGTGGTGTTG GACAGCTTTA TAAAGACCAG CGCTACTGGC
    GGTTTAGGAT CCATCAAAGC TGAAGTGATG GCTGACACTG CTGTAGCTTT GGCGTCAGGA
    AATGTCAAAC TGGTCTCCAG CAAGGTGATT GGCCGAATGT GTAAGATCAT CGACAAGACG
    TGTCTGTCTC CCACTCCGAC CCTTGAGCAG CACCTCATGT GGGACGACAT CGCCATCCTG
    GCCCGGTACA TGCTCATGCT GTCCTTCAAC AACTCTCTGG ACGTGGCCGC TCATCTGCCA
    TACCTGTTCC ACGTCGTCAC CCTGCTGGTG GCCACGGGCC CTCTCTCGCT GCGTGCATCA
    ACACACGGCC TGGTCATCAA CATCATTCAC TCGCTCTGCA CATGCTCACA ACTCAACTTC
    AGTGAGGAGA CTAAACAGGT GCTGCGCCTG AGTCTGACTG AGTTCTCCCT GCCCAAGTTC
    TACCTGCTGT TCGGCATCAG TAAGGTCAAA TCTGCGGCGG TCATCGCTTT CCGTTCCAGC
    TACAGAGACC GCTCATTCTC TCCAGGCTCT TATGAGAGAG AGACATTTGC TCTGTCTTCC
    CTGGAAACAG TCACGGAGGC TCTTTTGGAG ATCATGGAGG CCTGCATGAG GGACATTCCT
    GGATGCAAAT GGCTAGATCA GTGGACTGAA TTGGCGCAAA AGTTTGCGTT CCAGTATAAC
    CCCTCTCTTC AGCCTCGGGC GCTGGTGGTG TTCGGCTGCA TCAGTAAACG AGTGACTCAC
    GGACAGCTAA AGCAGATCAT TCGTATTCTC AGTAAAGCCA GTCCTCTACT CAGCATTGAT
    CGGGGGCTGG AGAGCTGCTT AAAGGGCCCA GATAATTACA ACAGCCAGGT TCTCATCGAA
    GCCACAGTCA TCGCTCTCAC CAAACTACAG CCTCTCCTCG ATCAGGATTC TCCTATGCAT
    AAAGCTCTGT TCTGGGTGGC CGTGGCCGTG CTTCAGTTGG ATGAAGTGAA TCTGTACTCC
    GCCGGCACTG CCTTACTGGA GCAGAACCTG CACACACTGG ACAGCCTCCG AGTGTTTAAT
    GATAAGAGTC CTGAAGAGGT TTTTATGGAG ATCCGGAAAC CTCTAGAGTG GCACTGTAAA
    CAAATGGACC ATTTTGTGGG ACTCAACTTT GCCTCCAACT TCAATTTTGC ATTAGTGGGC
    CATTTACTTA AAGGATACAG ACATCCCTCT TCAACAACAG TTGCCAGGAC AGTTCGTATC
    CTGCATACAC TCCTGGGCCT GGTGGGAAAA CACCGCAGCT GTGACAAGTT TGAGGTGAAC
    ACGCAGAGTG TGGCCTATCT GGCAGCTTTG CTCACAGTGT CAGAGGAAGT TAGGAGCCGC
    TGCAGTTTAA AGCACAGGAA ATCATTGCTG CTTTCAGAAG TTACAATGGA GAACTTCCCC
    ATGGACACAT ACACCGTTTA CGACACTGAA CCCGGGTGCA GGCTACTGCG TGAAAACCAG
    CCTTGGGCTT CTCCGAAAGT GTCAGAGCGC AGTCTTGCAC CTCCGTACTC CACAATAGGG
    CAGACCAGCC TTCGTCCACG CAAATCCATG AGTGTAGACA TGGGCCAGCC GTCTCAGGCC
    AATGCGAAGA AACTACTGGG TATTAGCGGC ACTAGAAAGA GTTTCGATCA CTTAATATCA
    GAATCCAAAG CCCCAAAAAG ACCAGACATG GAGTCGGGCA TCACCACACC TCCAAAGATG
    AGGCGGGTTT CTGAAAATGA CTATGAGATC GAAACTCAGC GAATAGCCAA CTCTCATTTA
    CGCAAAGTGT CTGTGTCTGA ATCCAATGTC CTCCTGGACG AGAACGTACT TACGGACCCC
    AAGATCCAGA CGTTGCTCCT CACTGTACTG GCAACACTGG TGAAGTACAC TACAGACGAG
    TTTCATCAGC GGATCTTGTA TGAGTATTTG GCTGAGGCCA GTGTGGTCTT CCCCAAGGTC
    TTTCCAGTTG TGCACAACCT GCTGGACTCC AAGTTAAGCA CCGTCCTTTC CATGTGCCAG
    GACCCGAACC TTCTGAACCC CGTCCATGGC ATCATTCAGA ACGTGGTCTA TCATGAGGAG
    TCTCCCCCTC AGTACCAGCC GTCCTGCTTG CAGAGCTTTG GTTTCAATGG TCTTTGGAGG
    TTTGCCGGGC CGTTCTCTAA GCAAACCCAA ATTCCAGACT ACGCCGAGCT GGTGGTTAAA
    TTTTTGGATG CTTTGATTGA CACGTATCTC CCGGGCATTG AGGAGGAAGG AGGAGAAGAG
    TCACTGCGGA CCCCGACCTC GCCATATCCA CCAACCGTCC AGAGCCAGCT GAGCATCACA
    GCCAATCTCA ACCTGTCCAA CTCCATGACA TCACTTGCCA CATCACAGCA CTCCCCAGCC
    TGTCTGCCTT GCTCTAAGTC GGCTGTATTC CCACCTCACC TCGTCCTGCC AGGCATCGAC
    AAGGAGAACG TTGAGATTTC TCCCACCTCG AGTCTGTCCA CCAGTGGGCG AATGCGGCAC
    GGCTCAGCAA GCCAGGTGCA GAAGCAGCGC AGCGCAGGCA GCTTCAAACG GCCAAGCATC
    AAAAAGATCG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017206684.1
    CDS253..8607
    Translation

    Target ORF information:

    RefSeq Version XM_017351195.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio neurofibromin 1a (nf1a), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351195.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    ATGGCGGCGC ACAAGCCCGT GGAATGGGTC CAGGCCATCA TTAACAGATT CGACGAACAG 
    CTCCCAATCA AAACAGGACA GCAGAGCACC CACACCAAAG TGAGTACAGA GCACAACAAA
    GAATGTTTGA TCAACATCTC CAAGTACAAA TTTGCCCTCG TCATCAATGG CCTGACCAAC
    ATACTCAAAA ATGTGAACAA CATGCGGATA TTTGGCGAAG CTGCTGAGAA GAACCTCTAC
    TTCTCCCAGC TGATCATACT GGACACACTG GAGAAGTGTC TAGCAGGGCA ACCTAAGGAT
    TGCCTGAGGC TGGATGAGGC TATGTTGGTA AAACAGTTGC TGCCTGAAAT CTGTCACTTC
    CTGCACTCGT ACCGTGAGGG CCAGCAACAC GCCACTCAGC TGCGCAGCTC CGCCTCTGCT
    GTGCTTTTCT CTCTCAGCTG CAACAACTTT AATGCCGTCT TCAGTCGTAT CTCCACCAGG
    TTGCAAGAGC TCACAGTTTG TACAGAGGAC ACAGTGGATG TCCATGACAT TGAGCTCATT
    CAGTATATCA ATGTAGATTG CTCCAAGTTA AAGAGACTCC TACAAGAAGC AGTACTTAAG
    TTCAAGTCTT TGAAAAAGCC GGCTCAGCTT GCAGTAATCA GCAGTTTGGA AAAGGCTTTC
    TGGAACTGGG TTGAACACTA CCCTGATGAT TTCACTATGC TCTATCAGAG ACCCCAGGCC
    GACATGGCCG ACTGTGCAGA GAGACTGTTC GACCTAGTGG ACAGTTTTGC GGAAAGCGCA
    AAGAGAAAAG CTGCTGTGTG GCCACTGCAG ATCATCCTCC TCATCCTCTG TCCAGAGATC
    ACGCAGAGCA TCACCAAAGA AGTAGGGGGA GAGGAGAAAG CTAACAAGAA GTCATTTGTA
    GACAGTTTAC GAAAGGCTCT TGGCCAACAC AGCGGCAGCA AACAATTGAC GGAAAGTGCA
    GCTGTTGCAT GCGTCAAACT CTGCAAAGCC TGCACATATA TCAACTGGGA GGATAATTCT
    GTCATCTTTC TCCTGGTTCA GTCCATCGTC ATGGACCTTA AGGGTCTCCT GTTTAACCCC
    ACCAAACCTT TCTCAAGAGG TACGGGCTGC CAGAGCGCCG ATGTAGATTT GATGATCGAC
    TGCTTTGTGT CATGTTATCG CATCAATCCT CGCAACAACC AGCATTTTAA GGTGTGTCTG
    GCCTCGACTT CTCCTCCTAC CTTCCATTTC GTCCTTGTCA ACTCCCTGCA CAAAATTATC
    ACAAATTCTC ACCTGGACTG GTGGCCGAAG ATTGACGCCA TCTACTGCTA CTCAGGAGAA
    CTACGCACCA TGTTTGCAGA CACACTGGGT CGAGTGATTC AGAGTCTCAG TGCACATCCG
    CCTCTGAGGA TGACATCAAG TTTGACATTC AAAGAGAAAA TGACAACAAG TTTGAAGTTC
    AAGGAAAAGT CCACAGATCT TGATTCGCGT TCTTTGCTGC TGGCACTGGT CAAACTTATC
    CACGCTGACC CCAAACTAAT GCTGCACAAT CCGGGAAAAC CTGGTCAGGA GATTCAGAAC
    AGCACAGCTG AGATCATCAA AGGTCTGGTT CAGCTGGTTC CTCTTACCCA CAGTGCTGAG
    CTTTCCCAAG AGGCCATGGA GGCATTGCTT GTTTTACATC AGCCAGAAAC CATTGAGCTA
    TGGAACCCGG ACGCTCCCAT TGAGACCTTC TGGGACATCA GCTCTCAGGT GTTATTTCTA
    ATTTGTAAGA AGCTGATTGG ACACCAGATG ATCAACAGTA CAGAGATACT CAAGTGGCTC
    AGGGAGATTC TCATATGCAG GAACAAGTTT CTATTAAAGA ACAAGGAGTG TGCTACACAG
    GGCAGTCTGA TCCCCATCAG CAGACAGGCT CAGACCAAGC TGGAGGTGTG TCTGTACACG
    TTCCTGTGGA GTCCTGATGC AGAGGCTGTT TTGGTGGCCA TGTCCTGTTT CAGGCATCTC
    TGTGAGGAGG CCGATATTCG CTGCAGTGTC GATGAGCTGC CTGTTCACAC AGTCCTACCC
    AATTACAACA CCTTCATTGA GCTTGCATCC GTCAGCAATA TGATGGCCAC AGGTCGCAAC
    ACACTACAAA AAAGAGTGAT GGCACTTTTA AGAAGAATAA AGCACCCTAC AGCTGGAAAT
    GTAGAGGCAT GGGAAGACAC ATATGCAAAG TGGGAGCAAG CTACAAAACA GATACTCAAC
    CACCCAAAAA ACAAAGTGGA AGATGGACAG GTAGTTAACA TAAGATTGGT CAATTATTTC
    ATCTTTTTTT CCATTCATGG TCATCTATTT TCTGTTCGTC CTCAGGAGTG GATCAACATG
    ACTGGTTTCT TGTGCGCTCT GGGAGGTGTT TGTCTCCAAC AGCGCAGCAC GCCGAGTCTG
    GGCACCTACA GTCCTCCAAT GGGCCCCCTG ACCGAACGCA AGGGGTCCAT GATTTCCATG
    AGCTCCTGCG AGGTAAACAG CGAAACATCC CTGAATCGCT TTCTGGAGCG TCTGCTGGCT
    CTGATGGTCT GCACACATGA GAAAGTGGGA GTGCAAATTC GCACCAACGT CAAAGACCTG
    GTGGGACTGG AGCTCAGTCC GGCTCTCTAT TCAATGCTCT TCACTAAGCT GCGCAACAGC
    ATCGGACGCT TCTTTGACAC CCAGGGGCCG GTTCCTATTA CAGATGCAAA CACTCTGTTT
    GTAGAGCAGA CCATTGCTAT CATGAAGAAC TTGCTGGACA GTCACACTGA AGGAAGCTCA
    GAGCACCTTG GTCAAGCCAG CATTGAGACC ATGATGCTCA ATCTAGTTAG GTATGTGCGT
    ATTTTAGGGA ACATTGTTCA TGCCATTCAG ATCAAAACCA AGTTGTGTCA GTTAGTGGAG
    GTGATGATGG AGCGCAGGGA TGACCTCTCC TTCTGCCAGG AGATGAAATT CAGGAATAAA
    ATGGTGGAGT ACCTCACAGA CTGGGTGATG GGAACCTCCA ATCAAGCTGC GGACGATGAT
    GTCAAATGCC TGACCCGCGA TCTGGACCAG GCCAGTATGG AGGCTGTCGT GTCTCTGTTG
    GCTGGTCTTC CTCTACAGCC TGAGGAGGGT GACGGTGTTG AGCTCATGGA GGCCAAATCA
    CAGCTCTTCC TGAAGTACTT CACACTGTTC ATGAACCTGT TGAATGACTG CAGCGAGGTT
    GAGGAAGATG GCCAGCAGGT CGCAGGGAGG AAACGTGGCA TGTCTAGACG ACTGGCATCT
    CTGCGGCACT GCACAGTATT GGCTATGTCT AACTTGCTCA ATGCTAATGT GGATAGCGGC
    CTCATGCACT CAATCGGTCT GGGATACCAT AAGGACCTGC AGACACGGGC TACATTTATG
    GAAGTGCTCA CTAAGATCCT TCAGCAAGGG ACGGAGTTCG ACACGCTTGC AGAGACTGTG
    CTTGCAGATC GCTTTGAGAG ACTGGTCGAG CTGGTCACCA TGATGGGAGA TCAGGGAGAG
    CTGCCCATAG CCATGGCTCT GGCCAATGTG GTCCCATGCT CTCAATGGGA TGAGCTGGCC
    AGAGTGTTAG TGACTCTCTT CGACTCTCGC CATCTGCTGT ATCAGCTGTT GTGGAATATG
    TTCTCCAAAG AGGTGGAGCT GGCAGACTCA ATGCAGACGC TCTTTAGAGG AAATAGCCTA
    GCCAGCAAGA TTATGACCTT CTGCTTCAAG GTGTACGGAG CTGCGTATCT ACAGAAGCTT
    CTGGAGCCGC TGTTGAGAGG AATCATCACT ACACCAGAGT GGCAGCAGAT CAGTTTTGAA
    GTGGACCCTA CTAGACTTGA ACAGGCAGAG AACCTGGAGG AGAACCAGAA GAACCTGCTT
    CAGATCACTG ACCGCTTCTT CCTGGCCATC ATCAACTCCT CGAGCGAGTT TCCTCCACAG
    CTGCGCAGTG TGTGCCACTG TCTGTATCAG GTGGTCAGTC AGCGGTTTCC TCAGAACAGC
    ATTGGTGCTG TAGGAAGTGC CATGTTCCTG CGCTTTATTA ACCCAGCTAT CGTGTCCCCA
    TATGAGGCTG GAATCCTAGA CAAGAAACCC CCACCTCGCA TCGAGCGTGG CCTTAAACTC
    ATGTCTAAGA TTCTGCAGAG CATTGCTAAT CATGTTCTCT TCACAAAAGA GGAGCATATG
    AGGCCCTTTA ATGATTTTGT CAAAAGCAAC TTCGATGCAG CCAGAAGGTT CTTCTTGGAT
    ATCGCATCTG ATTCTCCACC AAGTGATTCT GTGAACCATA GTCTGTCTTT CATCAGTGAC
    GGTAATGTGC TCGCTCTGCA CCGGCTATTA TGGAACAACC AGGAAAGAAT TGGCCAATAC
    CTGTCCAGCA ACAGGGACCA CAAGGCAGTG GGCCGTAGAC CATTTGACAA GATGGCCACT
    CTTCTGGCGT ACCTGGGTCC TCCTGAACAT AAGCCAGTAG CAGACACACA CTGGTCCAGC
    CTCAACCTCA CCAGCTCAAA GTTTGAGGAG TTCATGACAA GGCATCAAGT CCATGAGAAG
    GAGGAATTTA AAGCATTAAA AACCCTCAAC ATATTTTACC AAGCTGGCAC ATCTAAGAAC
    GGCAACCCTG TGTTTTATTA CATCGCACGC AGGTTCAAAA CTGGACAAAT CAATGGTGAT
    CTTCTGATCT ACCACGTCCT GCTGACGCTC AAGCCCTACT ATGCCAAGCA TTATGAGATC
    GTAGTTGACC TTACTCATGT GGGCCCCAGT AACCGTTTCA AGACGGACTT CTTATCCAAA
    TGGTTTGTGG TCTTTCCAAA CTTCGCCTAT GAGAACGTGG CTGCTGTGTA CATCTACAAC
    TGCAACACCT GGGTACGCGA GTACACCAAA TACCACGAGC GACTGCTGAC TGGCCTCAAG
    GGGAGCAAGA AGCTGGTGTT TATCGATTCG CCTGCACGTC TAGCTGAACA CATCGAACCC
    GAACAGCAGA AGCTGCCCGC AGCTACTTTA GCACTGGAAG AAGACCTCAA AGTGTTCCAC
    AACGCCCTAA AGCTTGCACA CAGAGACACA AAAGTGTCAA TTAAGGTGGG ATCCACAGCT
    GTACAGGTGA CCTCGGCCGA AAGAACGCGT GTCCTAGGAC AGCCTGTGTT CCTAAATGAC
    ATCTACTACG CTTCAGAGAT CGAGGAGATA TGCCTGGTGG ACGAGAACCA GTTCACGCTA
    ACCATCGCTA ACCAGGGCAC CCCACTCACT TTCATGCACC AGGAGTGTGA AGCCATCGTT
    CAGTCCATCA TCCACATCCG CACGCGCTGG GAACTTTCCC AACCAGACTC CATTCCCCAA
    CACACCAAAA TCAGGCCTAA GGATGTTCCT GGCACCCTGC TCAACATCGC TCTTCTCAAC
    CTGGGAAGCT CCGACCCCAG TCTGAGGTCT GCGGCCTACA ATCTCCTTTG TGCCTTAACC
    TGCACCTTCA ACCTGAAGAT TGAAGGCCAG CTTCTGGAAA CGTCTGGCTT GTGCATCCCA
    GCCAACAACA CGCTTTTTAT TGTGTCCATT AGCAAAACGC TAGCAGCTAA TGAACCCCAC
    CTCACTCTGG AGTTCCTGGA AGAGTGCATC TCAGGATTCA GTAAATCAAG TATAGAGCTG
    AAGCATCTGT GTCTAGAATA TATGACTCCT TGGCTCCGTA ATTTGGTGCG CTTTTGCAAA
    CACAATGATG ACGCCAAACG GCAGCGTGTC ACGGCCATCC TGGACAAGCT TATTACTATG
    ACCATCAACG AGAAGCAGAT GTACCCCTCC ATACAGGCCA AGATATGGGG CAGCTTGGGT
    CAGATCACAG ACCTGCTTGA TGTGGTGTTG GACAGCTTTA TAAAGACCAG CGCTACTGGC
    GGTTTAGGAT CCATCAAAGC TGAAGTGATG GCTGACACTG CTGTAGCTTT GGCGTCAGGA
    AATGTCAAAC TGGTCTCCAG CAAGGTGATT GGCCGAATGT GTAAGATCAT CGACAAGACG
    TGTCTGTCTC CCACTCCGAC CCTTGAGCAG CACCTCATGT GGGACGACAT CGCCATCCTG
    GCCCGGTACA TGCTCATGCT GTCCTTCAAC AACTCTCTGG ACGTGGCCGC TCATCTGCCA
    TACCTGTTCC ACGTCGTCAC CCTGCTGGTG GCCACGGGCC CTCTCTCGCT GCGTGCATCA
    ACACACGGCC TGGTCATCAA CATCATTCAC TCGCTCTGCA CATGCTCACA ACTCAACTTC
    AGTGAGGAGA CTAAACAGGT GCTGCGCCTG AGTCTGACTG AGTTCTCCCT GCCCAAGTTC
    TACCTGCTGT TCGGCATCAG TAAGGTCAAA TCTGCGGCGG TCATCGCTTT CCGTTCCAGC
    TACAGAGACC GCTCATTCTC TCCAGGCTCT TATGAGAGAG AGACATTTGC TCTGTCTTCC
    CTGGAAACAG TCACGGAGGC TCTTTTGGAG ATCATGGAGG CCTGCATGAG GGACATTCCT
    GGATGCAAAT GGCTAGATCA GTGGACTGAA TTGGCGCAAA AGTTTGCGTT CCAGTATAAC
    CCCTCTCTTC AGCCTCGGGC GCTGGTGGTG TTCGGCTGCA TCAGTAAACG AGTGACTCAC
    GGACAGCTAA AGCAGATCAT TCGTATTCTC AGTAAAGCCA GTCCTCTACT CAGCATTGAT
    CGGGGGCTGG AGAGCTGCTT AAAGGGCCCA GATAATTACA ACAGCCAGGT TCTCATCGAA
    GCCACAGTCA TCGCTCTCAC CAAACTACAG CCTCTCCTCG ATCAGGATTC TCCTATGCAT
    AAAGCTCTGT TCTGGGTGGC CGTGGCCGTG CTTCAGTTGG ATGAAGTGAA TCTGTACTCC
    GCCGGCACTG CCTTACTGGA GCAGAACCTG CACACACTGG ACAGCCTCCG AGTGTTTAAT
    GATAAGAGTC CTGAAGAGGT TTTTATGGAG ATCCGGAAAC CTCTAGAGTG GCACTGTAAA
    CAAATGGACC ATTTTGTGGG ACTCAACTTT GCCTCCAACT TCAATTTTGC ATTAGTGGGC
    CATTTACTTA AAGGATACAG ACATCCCTCT TCAACAACAG TTGCCAGGAC AGTTCGTATC
    CTGCATACAC TCCTGGGCCT GGTGGGAAAA CACCGCAGCT GTGACAAGTT TGAGGTGAAC
    ACGCAGAGTG TGGCCTATCT GGCAGCTTTG CTCACAGTGT CAGAGGAAGT TAGGAGCCGC
    TGCAGTTTAA AGCACAGGAA ATCATTGCTG CTTTCAGAAG TTACAATGGA GAACTTCCCC
    ATGGACACAT ACACCGTTTA CGACACTGAA CCCGGGTGCA GGCTACTGCG TGAAAACCAG
    CCTTGGGCTT CTCCGAAAGT GTCAGAGCGC AGTCTTGCAC CTCCGTACTC CACAATAGGG
    CAGACCAGCC TTCGTCCACG CAAATCCATG AGTGTAGACA TGGGCCAGCC GTCTCAGGCC
    AATGCGAAGA AACTACTGGG TATTAGCGGC ACTAGAAAGA GTTTCGATCA CTTAATATCA
    GAATCCAAAG CCCCAAAAAG ACCAGACATG GAGTCGGGCA TCACCACACC TCCAAAGATG
    AGGCGGGTTT CTGAAAATGA CTATGAGATC GAAACTCAGC GAATAGCCAA CTCTCATTTA
    CGCAAAGTGT CTGTGTCTGA ATCCAATGTC CTCCTGGACG AGAACGTACT TACGGACCCC
    AAGATCCAGA CGTTGCTCCT CACTGTACTG GCAACACTGG TGAAGTACAC TACAGACGAG
    TTTCATCAGC GGATCTTGTA TGAGTATTTG GCTGAGGCCA GTGTGGTCTT CCCCAAGGTC
    TTTCCAGTTG TGCACAACCT GCTGGACTCC AAGTTAAGCA CCGTCCTTTC CATGTGCCAG
    GACCCGAACC TTCTGAACCC CGTCCATGGC ATCATTCAGA ACGTGGTCTA TCATGAGGAG
    TCTCCCCCTC AGTACCAGCC GTCCTGCTTG CAGAGCTTTG GTTTCAATGG TCTTTGGAGG
    TTTGCCGGGC CGTTCTCTAA GCAAACCCAA ATTCCAGACT ACGCCGAGCT GGTGGTTAAA
    TTTTTGGATG CTTTGATTGA CACGTATCTC CCGGGCATTG AGGAGGAAGG AGGAGAAGAG
    TCACTGCGGA CCCCGACCTC GCCATATCCA CCAACCGTCC AGAGCCAGCT GAGCATCACA
    GCCAATCTCA ACCTGTCCAA CTCCATGACA TCACTTGCCA CATCACAGCA CTCCCCAGCC
    TGTCTGCCTT GCTCTAAGTC GGCTGTATTC CCACCTCACC TCGTCCTGCC AGGCATCGAC
    AAGGAGAACG TTGAGATTTC TCCCACCTCG AGTCTGTCCA CCAGTGGGCG AATGCGGCAC
    GGCTCAGCAA GCCAGGTGCA GAAGCAGCGC AGCGCAGGCA GCTTCAAACG GCCAAGCATC
    AAAAAGATCG TCTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55273
    Clone ID Related Accession (Same CDS sequence) XM_017351196.1
    Accession Version XM_017351196.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8301bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product neurofibromin isoform X7
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
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    1321
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    1501
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    ATGGCGGCGC ACAAGCCCGT GGAATGGGTC CAGGCCATCA TTAACAGATT CGACGAACAG 
    CTCCCAATCA AAACAGGACA GCAGAGCACC CACACCAAAG TGAGTACAGA GCACAACAAA
    GAATGTTTGA TCAACATCTC CAAGTACAAA TTTGCCCTCG TCATCAATGG CCTGACCAAC
    ATACTCAAAA ATGTGAACAA CATGCGGATA TTTGGCGAAG CTGCTGAGAA GAACCTCTAC
    TTCTCCCAGC TGATCATACT GGACACACTG GAGAAGTGTC TAGCAGGGCA ACCTAAGGAT
    TGCCTGAGGC TGGATGAGGC TATGTTGGTA AAACAGTTGC TGCCTGAAAT CTGTCACTTC
    CTGCACTCGT ACCGTGAGGG CCAGCAACAC GCCACTCAGC TGCGCAGCTC CGCCTCTGCT
    GTGCTTTTCT CTCTCAGCTG CAACAACTTT AATGCCGTCT TCAGTCGTAT CTCCACCAGG
    TTGCAAGAGC TCACAGTTTG TACAGAGGAC ACAGTGGATG TCCATGACAT TGAGCTCATT
    CAGTATATCA ATGTAGATTG CTCCAAGTTA AAGAGACTCC TACAAGAAGC AGTACTTAAG
    TTCAAGTCTT TGAAAAAGCC GGCTCAGCTT GCAGTAATCA GCAGTTTGGA AAAGGCTTTC
    TGGAACTGGG TTGAACACTA CCCTGATGAT TTCACTATGC TCTATCAGAG ACCCCAGGCC
    GACATGGCCG ACTGTGCAGA GAGACTGTTC GACCTAGTGG ACAGTTTTGC GGAAAGCGCA
    AAGAGAAAAG CTGCTGTGTG GCCACTGCAG ATCATCCTCC TCATCCTCTG TCCAGAGATC
    ACGCAGAGCA TCACCAAAGA AGTAGGGGGA GAGGAGAAAG CTAACAAGAA GTCATTTGTA
    GACAGTTTAC GAAAGGCTCT TGGCCAACAC AGCGGCAGCA AACAATTGAC GGAAAGTGCA
    GCTGTTGCAT GCGTCAAACT CTGCAAAGCC TGCACATATA TCAACTGGGA GGATAATTCT
    GTCATCTTTC TCCTGGTTCA GTCCATCGTC ATGGACCTTA AGGGTCTCCT GTTTAACCCC
    ACCAAACCTT TCTCAAGAGG TACGGGCTGC CAGAGCGCCG ATGTAGATTT GATGATCGAC
    TGCTTTGTGT CATGTTATCG CATCAATCCT CGCAACAACC AGCATTTTAA GGTGTGTCTG
    GCCTCGACTT CTCCTCCTAC CTTCCATTTC GTCCTTGTCA ACTCCCTGCA CAAAATTATC
    ACAAATTCTC ACCTGGACTG GTGGCCGAAG ATTGACGCCA TCTACTGCTA CTCAGGAGAA
    CTACGCACCA TGTTTGCAGA CACACTGGGT CGAGTGATTC AGAGTCTCAG TGCACATCCG
    CCTCTGAGGA TGACATCAAG TTTGACATTC AAAGAGAAAA TGACAACAAG TTTGAAGTTC
    AAGGAAAAGT CCACAGATCT TGATTCGCGT TCTTTGCTGC TGGCACTGGT CAAACTTATC
    CACGCTGACC CCAAACTAAT GCTGCACAAT CCGGGAAAAC CTGGTCAGGA GATTCAGAAC
    AGCACAGCTG AGATCATCAA AGGTCTGGTT CAGCTGGTTC CTCTTACCCA CAGTGCTGAG
    CTTTCCCAAG AGGCCATGGA GGCATTGCTT GTTTTACATC AGCCAGAAAC CATTGAGCTA
    TGGAACCCGG ACGCTCCCAT TGAGACCTTC TGGGACATCA GCTCTCAGGT GTTATTTCTA
    ATTTGTAAGA AGCTGATTGG ACACCAGATG ATCAACAGTA CAGAGATACT CAAGTGGCTC
    AGGGAGATTC TCATATGCAG GAACAAGTTT CTATTAAAGA ACAAGGAGTG TGCTACACAG
    GGCAGTCTGA TCCCCATCAG CAGACAGGCT CAGACCAAGC TGGAGGTGTG TCTGTACACG
    TTCCTGTGGA GTCCTGATGC AGAGGCTGTT TTGGTGGCCA TGTCCTGTTT CAGGCATCTC
    TGTGAGGAGG CCGATATTCG CTGCAGTGTC GATGAGCTGC CTGTTCACAC AGTCCTACCC
    AATTACAACA CCTTCATTGA GCTTGCATCC GTCAGCAATA TGATGGCCAC AGGTCGCAAC
    ACACTACAAA AAAGAGTGAT GGCACTTTTA AGAAGAATAA AGCACCCTAC AGCTGGAAAT
    GTAGAGGCAT GGGAAGACAC ATATGCAAAG TGGGAGCAAG CTACAAAACA GATACTCAAC
    CACCCAAAAA ACAAAGTGGA AGATGGACAG GTAGTTAACA TAAGATTGGT CAATTATTTC
    ATCTTTTTTT CCATTCATGG TCATCTATTT TCTGTTCGTC CTCAGGAGTG GATCAACATG
    ACTGGTTTCT TGTGCGCTCT GGGAGGTGTT TGTCTCCAAC AGCGCAGCAC GCCGAGTCTG
    GGCACCTACA GTCCTCCAAT GGGCCCCCTG ACCGAACGCA AGGGGTCCAT GATTTCCATG
    AGCTCCTGCG AGGTAAACAG CGAAACATCC CTGAATCGCT TTCTGGAGCG TCTGCTGGCT
    CTGATGGTCT GCACACATGA GAAAGTGGGA GTGCAAATTC GCACCAACGT CAAAGACCTG
    GTGGGACTGG AGCTCAGTCC GGCTCTCTAT TCAATGCTCT TCACTAAGCT GCGCAACAGC
    ATCGGACGCT TCTTTGACAC CCAGGGGCCG GTTCCTATTA CAGATGCAAA CACTCTGTTT
    GTAGAGCAGA CCATTGCTAT CATGAAGAAC TTGCTGGACA GTCACACTGA AGGAAGCTCA
    GAGCACCTTG GTCAAGCCAG CATTGAGACC ATGATGCTCA ATCTAGTTAG GTATGTGCGT
    ATTTTAGGGA ACATTGTTCA TGCCATTCAG ATCAAAACCA AGTTGTGTCA GTTAGTGGAG
    GTGATGATGG AGCGCAGGGA TGACCTCTCC TTCTGCCAGG AGATGAAATT CAGGAATAAA
    ATGGTGGAGT ACCTCACAGA CTGGGTGATG GGAACCTCCA ATCAAGCTGC GGACGATGAT
    GTCAAATGCC TGACCCGCGA TCTGGACCAG GCCAGTATGG AGGCTGTCGT GTCTCTGTTG
    GCTGGTCTTC CTCTACAGCC TGAGGAGGGT GACGGTGTTG AGCTCATGGA GGCCAAATCA
    CAGCTCTTCC TGAAGTACTT CACACTGTTC ATGAACCTGT TGAATGACTG CAGCGAGGTT
    GAGGAAGATG GCCAGCAGGT CGCAGGGAGG AAACGTGGCA TGTCTAGACG ACTGGCATCT
    CTGCGGCACT GCACAGTATT GGCTATGTCT AACTTGCTCA ATGCTAATGT GGATAGCGGC
    CTCATGCACT CAATCGGTCT GGGATACCAT AAGGACCTGC AGACACGGGC TACATTTATG
    GAAGTGCTCA CTAAGATCCT TCAGCAAGGG ACGGAGTTCG ACACGCTTGC AGAGACTGTG
    CTTGCAGATC GCTTTGAGAG ACTGGTCGAG CTGGTCACCA TGATGGGAGA TCAGGGAGAG
    CTGCCCATAG CCATGGCTCT GGCCAATGTG GTCCCATGCT CTCAATGGGA TGAGCTGGCC
    AGAGTGTTAG TGACTCTCTT CGACTCTCGC CATCTGCTGT ATCAGCTGTT GTGGAATATG
    TTCTCCAAAG AGGTGGAGCT GGCAGACTCA ATGCAGACGC TCTTTAGAGG AAATAGCCTA
    GCCAGCAAGA TTATGACCTT CTGCTTCAAG GTGTACGGAG CTGCGTATCT ACAGAAGCTT
    CTGGAGCCGC TGTTGAGAGG AATCATCACT ACACCAGAGT GGCAGCAGAT CAGTTTTGAA
    GTGGACCCTA CTAGACTTGA ACAGGCAGAG AACCTGGAGG AGAACCAGAA GAACCTGCTT
    CAGATCACTG ACCGCTTCTT CCTGGCCATC ATCAACTCCT CGAGCGAGTT TCCTCCACAG
    CTGCGCAGTG TGTGCCACTG TCTGTATCAG GTGGTCAGTC AGCGGTTTCC TCAGAACAGC
    ATTGGTGCTG TAGGAAGTGC CATGTTCCTG CGCTTTATTA ACCCAGCTAT CGTGTCCCCA
    TATGAGGCTG GAATCCTAGA CAAGAAACCC CCACCTCGCA TCGAGCGTGG CCTTAAACTC
    ATGTCTAAGA TTCTGCAGAG CATTGCTAAT CATGTTCTCT TCACAAAAGA GGAGCATATG
    AGGCCCTTTA ATGATTTTGT CAAAAGCAAC TTCGATGCAG CCAGAAGGTT CTTCTTGGAT
    ATCGCATCTG ATTCTCCACC AAGTGATTCT GTGAACCATA GTCTGTCTTT CATCAGTGAC
    GGTAATGTGC TCGCTCTGCA CCGGCTATTA TGGAACAACC AGGAAAGAAT TGGCCAATAC
    CTGTCCAGCA ACAGGGACCA CAAGGCAGTG GGCCGTAGAC CATTTGACAA GATGGCCACT
    CTTCTGGCGT ACCTGGGTCC TCCTGAACAT AAGCCAGTAG CAGACACACA CTGGTCCAGC
    CTCAACCTCA CCAGCTCAAA GTTTGAGGAG TTCATGACAA GGCATCAAGT CCATGAGAAG
    GAGGAATTTA AAGCATTAAA AACCCTCAAC ATATTTTACC AAGCTGGCAC ATCTAAGAAC
    GGCAACCCTG TGTTTTATTA CATCGCACGC AGGTTCAAAA CTGGACAAAT CAATGGTGAT
    CTTCTGATCT ACCACGTCCT GCTGACGCTC AAGCCCTACT ATGCCAAGCA TTATGAGATC
    GTAGTTGACC TTACTCATGT GGGCCCCAGT AACCGTTTCA AGACGGACTT CTTATCCAAA
    TGGTTTGTGG TCTTTCCAAA CTTCGCCTAT GAGAACGTGG CTGCTGTGTA CATCTACAAC
    TGCAACACCT GGGTACGCGA GTACACCAAA TACCACGAGC GACTGCTGAC TGGCCTCAAG
    GGGAGCAAGA AGCTGGTGTT TATCGATTCG CCTGCACGTC TAGCTGAACA CATCGAACCC
    GAACAGCAGA AGCTGCCCGC AGCTACTTTA GCACTGGAAG AAGACCTCAA AGTGTTCCAC
    AACGCCCTAA AGCTTGCACA CAGAGACACA AAAGTGTCAA TTAAGGTGGG ATCCACAGCT
    GTACAGGTGA CCTCGGCCGA AAGAACGCGT GTCCTAGGAC AGCCTGTGTT CCTAAATGAC
    ATCTACTACG CTTCAGAGAT CGAGGAGATA TGCCTGGTGG ACGAGAACCA GTTCACGCTA
    ACCATCGCTA ACCAGGGCAC CCCACTCACT TTCATGCACC AGGAGTGTGA AGCCATCGTT
    CAGTCCATCA TCCACATCCG CACGCGCTGG GAACTTTCCC AACCAGACTC CATTCCCCAA
    CACACCAAAA TCAGGCCTAA GGATGTTCCT GGCACCCTGC TCAACATCGC TCTTCTCAAC
    CTGGGAAGCT CCGACCCCAG TCTGAGGTCT GCGGCCTACA ATCTCCTTTG TGCCTTAACC
    TGCACCTTCA ACCTGAAGAT TGAAGGCCAG CTTCTGGAAA CGTCTGGCTT GTGCATCCCA
    GCCAACAACA CGCTTTTTAT TGTGTCCATT AGCAAAACGC TAGCAGCTAA TGAACCCCAC
    CTCACTCTGG AGTTCCTGGA AGAGTGCATC TCAGGATTCA GTAAATCAAG TATAGAGCTG
    AAGCATCTGT GTCTAGAATA TATGACTCCT TGGCTCCGTA ATTTGGTGCG CTTTTGCAAA
    CACAATGATG ACGCCAAACG GCAGCGTGTC ACGGCCATCC TGGACAAGCT TATTACTATG
    ACCATCAACG AGAAGCAGAT GTACCCCTCC ATACAGGCCA AGATATGGGG CAGCTTGGGT
    CAGATCACAG ACCTGCTTGA TGTGGTGTTG GACAGCTTTA TAAAGACCAG CGCTACTGGC
    GGTTTAGGAT CCATCAAAGC TGAAGTGATG GCTGACACTG CTGTAGCTTT GGCGTCAGGA
    AATGTCAAAC TGGTCTCCAG CAAGGTGATT GGCCGAATGT GTAAGATCAT CGACAAGACG
    TGTCTGTCTC CCACTCCGAC CCTTGAGCAG CACCTCATGT GGGACGACAT CGCCATCCTG
    GCCCGGTACA TGCTCATGCT GTCCTTCAAC AACTCTCTGG ACGTGGCCGC TCATCTGCCA
    TACCTGTTCC ACGTCGTCAC CCTGCTGGTG GCCACGGGCC CTCTCTCGCT GCGTGCATCA
    ACACACGGCC TGGTCATCAA CATCATTCAC TCGCTCTGCA CATGCTCACA ACTCAACTTC
    AGTGAGGAGA CTAAACAGGT GCTGCGCCTG AGTCTGACTG AGTTCTCCCT GCCCAAGTTC
    TACCTGCTGT TCGGCATCAG TAAGGTCAAA TCTGCGGCGG TCATCGCTTT CCGTTCCAGC
    TACAGAGACC GCTCATTCTC TCCAGGCTCT TATGAGAGAG AGACATTTGC TCTGTCTTCC
    CTGGAAACAG TCACGGAGGC TCTTTTGGAG ATCATGGAGG CCTGCATGAG GGACATTCCT
    GGATGCAAAT GGCTAGATCA GTGGACTGAA TTGGCGCAAA AGTTTGCGTT CCAGTATAAC
    CCCTCTCTTC AGCCTCGGGC GCTGGTGGTG TTCGGCTGCA TCAGTAAACG AGTGACTCAC
    GGACAGCTAA AGCAGATCAT TCGTATTCTC AGTAAAGCCA GTCCTCTACT CAGCATTGAT
    CGGGGGCTGG AGAGCTGCTT AAAGGGCCCA GATAATTACA ACAGCCAGGT TCTCATCGAA
    GCCACAGTCA TCGCTCTCAC CAAACTACAG CCTCTCCTCG ATCAGGATTC TCCTATGCAT
    AAAGCTCTGT TCTGGGTGGC CGTGGCCGTG CTTCAGTTGG ATGAAGTGAA TCTGTACTCC
    GCCGGCACTG CCTTACTGGA GCAGAACCTG CACACACTGG ACAGCCTCCG AGTGTTTAAT
    GATAAGAGTC CTGAAGAGGT TTTTATGGAG ATCCGGAAAC CTCTAGAGTG GCACTGTAAA
    CAAATGGACC ATTTTGTGGG ACTCAACTTT GCCTCCAACT TCAATTTTGC ATTAGTGGGC
    CATTTACTTA AAGGATACAG ACATCCCTCT TCAACAACAG TTGCCAGGAC AGTTCGTATC
    CTGCATACAC TCCTGGGCCT GGTGGGAAAA CACCGCAGCT GTGACAAGTT TGAGGTGAAC
    ACGCAGAGTG TGGCCTATCT GGCAGCTTTG CTCACAGTGT CAGAGGAAGT TAGGAGCCGC
    TGCAGTTTAA AGCACAGGAA ATCATTGCTG CTTTCAGAAG TTACAATGGA GAACTTCCCC
    ATGGACACAT ACACCGTTTA CGACACTGAA CCCGGGTGCA GGCTACTGCG TGAAAACCAG
    CCTTGGGCTT CTCCGAAAGT GTCAGAGCGC AGTCTTGCAC CTCCGTACTC CACAATAGGG
    CAGACCAGCC TTCGTCCACG CAAATCCATG AGTGTAGACA TGGGCCAGCC GTCTCAGGCC
    AATGCGAAGA AACTACTGGG TATTAGCGGC ACTAGAAAGA GTTTCGATCA CTTAATATCA
    GAATCCAAAG CCCCAAAAAG ACCAGACATG GAGTCGGGCA TCACCACACC TCCAAAGATG
    AGGCGGGTTT CTGAAAATGA CTATGAGATC GAAACTCAGC GAATAGCCAA CTCTCATTTA
    CGCAAAGTGT CTGTGTCTGA ATCCAATGTC CTCCTGGACG AGAACGTACT TACGGACCCC
    AAGATCCAGA CGTTGCTCCT CACTGTACTG GCAACACTGG TGAAGTACAC TACAGACGAG
    TTTCATCAGC GGATCTTGTA TGAGTATTTG GCTGAGGCCA GTGTGGTCTT CCCCAAGGTC
    TTTCCAGTTG TGCACAACCT GCTGGACTCC AAGTTAAGCA CCGTCCTTTC CATGTGCCAG
    GACCCGAACC TTCTGAACCC CGTCCATGGC ATCATTCAGA ACGTGGTCTA TCATGAGGAG
    TCTCCCCCTC AGTACCAGCC GTCCTGCTTG CAGAGCTTTG GTTTCAATGG TCTTTGGAGG
    TTTGCCGGGC CGTTCTCTAA GCAAACCCAA ATTCCAGACT ACGCCGAGCT GGTGGTTAAA
    TTTTTGGATG CTTTGATTGA CACGTATCTC CCGGGCATTG AGGAGGAAGG AGGAGAAGAG
    TCACTGCGGA CCCCGACCTC GCCATATCCA CCAACCGTCC AGAGCCAGCT GAGCATCACA
    GCCAATCTCA ACCTGTCCAA CTCCATGACA TCACTTGCCA CATCACAGCA CTCCCCAGGC
    ATCGACAAGG AGAACGTTGA GATTTCTCCC ACCTCGAGTC TGTCCACCAG TGGGCGAATG
    CGGCACGGCT CAGCAAGCCA GGTGCAGAAG CAGCGCAGCG CAGGCAGCTT CAAACGGCCA
    AGCATCAAAA AGATCGTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017206685.1
    CDS253..8553
    Translation

    Target ORF information:

    RefSeq Version XM_017351196.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio neurofibromin 1a (nf1a), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351196.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    ATGGCGGCGC ACAAGCCCGT GGAATGGGTC CAGGCCATCA TTAACAGATT CGACGAACAG 
    CTCCCAATCA AAACAGGACA GCAGAGCACC CACACCAAAG TGAGTACAGA GCACAACAAA
    GAATGTTTGA TCAACATCTC CAAGTACAAA TTTGCCCTCG TCATCAATGG CCTGACCAAC
    ATACTCAAAA ATGTGAACAA CATGCGGATA TTTGGCGAAG CTGCTGAGAA GAACCTCTAC
    TTCTCCCAGC TGATCATACT GGACACACTG GAGAAGTGTC TAGCAGGGCA ACCTAAGGAT
    TGCCTGAGGC TGGATGAGGC TATGTTGGTA AAACAGTTGC TGCCTGAAAT CTGTCACTTC
    CTGCACTCGT ACCGTGAGGG CCAGCAACAC GCCACTCAGC TGCGCAGCTC CGCCTCTGCT
    GTGCTTTTCT CTCTCAGCTG CAACAACTTT AATGCCGTCT TCAGTCGTAT CTCCACCAGG
    TTGCAAGAGC TCACAGTTTG TACAGAGGAC ACAGTGGATG TCCATGACAT TGAGCTCATT
    CAGTATATCA ATGTAGATTG CTCCAAGTTA AAGAGACTCC TACAAGAAGC AGTACTTAAG
    TTCAAGTCTT TGAAAAAGCC GGCTCAGCTT GCAGTAATCA GCAGTTTGGA AAAGGCTTTC
    TGGAACTGGG TTGAACACTA CCCTGATGAT TTCACTATGC TCTATCAGAG ACCCCAGGCC
    GACATGGCCG ACTGTGCAGA GAGACTGTTC GACCTAGTGG ACAGTTTTGC GGAAAGCGCA
    AAGAGAAAAG CTGCTGTGTG GCCACTGCAG ATCATCCTCC TCATCCTCTG TCCAGAGATC
    ACGCAGAGCA TCACCAAAGA AGTAGGGGGA GAGGAGAAAG CTAACAAGAA GTCATTTGTA
    GACAGTTTAC GAAAGGCTCT TGGCCAACAC AGCGGCAGCA AACAATTGAC GGAAAGTGCA
    GCTGTTGCAT GCGTCAAACT CTGCAAAGCC TGCACATATA TCAACTGGGA GGATAATTCT
    GTCATCTTTC TCCTGGTTCA GTCCATCGTC ATGGACCTTA AGGGTCTCCT GTTTAACCCC
    ACCAAACCTT TCTCAAGAGG TACGGGCTGC CAGAGCGCCG ATGTAGATTT GATGATCGAC
    TGCTTTGTGT CATGTTATCG CATCAATCCT CGCAACAACC AGCATTTTAA GGTGTGTCTG
    GCCTCGACTT CTCCTCCTAC CTTCCATTTC GTCCTTGTCA ACTCCCTGCA CAAAATTATC
    ACAAATTCTC ACCTGGACTG GTGGCCGAAG ATTGACGCCA TCTACTGCTA CTCAGGAGAA
    CTACGCACCA TGTTTGCAGA CACACTGGGT CGAGTGATTC AGAGTCTCAG TGCACATCCG
    CCTCTGAGGA TGACATCAAG TTTGACATTC AAAGAGAAAA TGACAACAAG TTTGAAGTTC
    AAGGAAAAGT CCACAGATCT TGATTCGCGT TCTTTGCTGC TGGCACTGGT CAAACTTATC
    CACGCTGACC CCAAACTAAT GCTGCACAAT CCGGGAAAAC CTGGTCAGGA GATTCAGAAC
    AGCACAGCTG AGATCATCAA AGGTCTGGTT CAGCTGGTTC CTCTTACCCA CAGTGCTGAG
    CTTTCCCAAG AGGCCATGGA GGCATTGCTT GTTTTACATC AGCCAGAAAC CATTGAGCTA
    TGGAACCCGG ACGCTCCCAT TGAGACCTTC TGGGACATCA GCTCTCAGGT GTTATTTCTA
    ATTTGTAAGA AGCTGATTGG ACACCAGATG ATCAACAGTA CAGAGATACT CAAGTGGCTC
    AGGGAGATTC TCATATGCAG GAACAAGTTT CTATTAAAGA ACAAGGAGTG TGCTACACAG
    GGCAGTCTGA TCCCCATCAG CAGACAGGCT CAGACCAAGC TGGAGGTGTG TCTGTACACG
    TTCCTGTGGA GTCCTGATGC AGAGGCTGTT TTGGTGGCCA TGTCCTGTTT CAGGCATCTC
    TGTGAGGAGG CCGATATTCG CTGCAGTGTC GATGAGCTGC CTGTTCACAC AGTCCTACCC
    AATTACAACA CCTTCATTGA GCTTGCATCC GTCAGCAATA TGATGGCCAC AGGTCGCAAC
    ACACTACAAA AAAGAGTGAT GGCACTTTTA AGAAGAATAA AGCACCCTAC AGCTGGAAAT
    GTAGAGGCAT GGGAAGACAC ATATGCAAAG TGGGAGCAAG CTACAAAACA GATACTCAAC
    CACCCAAAAA ACAAAGTGGA AGATGGACAG GTAGTTAACA TAAGATTGGT CAATTATTTC
    ATCTTTTTTT CCATTCATGG TCATCTATTT TCTGTTCGTC CTCAGGAGTG GATCAACATG
    ACTGGTTTCT TGTGCGCTCT GGGAGGTGTT TGTCTCCAAC AGCGCAGCAC GCCGAGTCTG
    GGCACCTACA GTCCTCCAAT GGGCCCCCTG ACCGAACGCA AGGGGTCCAT GATTTCCATG
    AGCTCCTGCG AGGTAAACAG CGAAACATCC CTGAATCGCT TTCTGGAGCG TCTGCTGGCT
    CTGATGGTCT GCACACATGA GAAAGTGGGA GTGCAAATTC GCACCAACGT CAAAGACCTG
    GTGGGACTGG AGCTCAGTCC GGCTCTCTAT TCAATGCTCT TCACTAAGCT GCGCAACAGC
    ATCGGACGCT TCTTTGACAC CCAGGGGCCG GTTCCTATTA CAGATGCAAA CACTCTGTTT
    GTAGAGCAGA CCATTGCTAT CATGAAGAAC TTGCTGGACA GTCACACTGA AGGAAGCTCA
    GAGCACCTTG GTCAAGCCAG CATTGAGACC ATGATGCTCA ATCTAGTTAG GTATGTGCGT
    ATTTTAGGGA ACATTGTTCA TGCCATTCAG ATCAAAACCA AGTTGTGTCA GTTAGTGGAG
    GTGATGATGG AGCGCAGGGA TGACCTCTCC TTCTGCCAGG AGATGAAATT CAGGAATAAA
    ATGGTGGAGT ACCTCACAGA CTGGGTGATG GGAACCTCCA ATCAAGCTGC GGACGATGAT
    GTCAAATGCC TGACCCGCGA TCTGGACCAG GCCAGTATGG AGGCTGTCGT GTCTCTGTTG
    GCTGGTCTTC CTCTACAGCC TGAGGAGGGT GACGGTGTTG AGCTCATGGA GGCCAAATCA
    CAGCTCTTCC TGAAGTACTT CACACTGTTC ATGAACCTGT TGAATGACTG CAGCGAGGTT
    GAGGAAGATG GCCAGCAGGT CGCAGGGAGG AAACGTGGCA TGTCTAGACG ACTGGCATCT
    CTGCGGCACT GCACAGTATT GGCTATGTCT AACTTGCTCA ATGCTAATGT GGATAGCGGC
    CTCATGCACT CAATCGGTCT GGGATACCAT AAGGACCTGC AGACACGGGC TACATTTATG
    GAAGTGCTCA CTAAGATCCT TCAGCAAGGG ACGGAGTTCG ACACGCTTGC AGAGACTGTG
    CTTGCAGATC GCTTTGAGAG ACTGGTCGAG CTGGTCACCA TGATGGGAGA TCAGGGAGAG
    CTGCCCATAG CCATGGCTCT GGCCAATGTG GTCCCATGCT CTCAATGGGA TGAGCTGGCC
    AGAGTGTTAG TGACTCTCTT CGACTCTCGC CATCTGCTGT ATCAGCTGTT GTGGAATATG
    TTCTCCAAAG AGGTGGAGCT GGCAGACTCA ATGCAGACGC TCTTTAGAGG AAATAGCCTA
    GCCAGCAAGA TTATGACCTT CTGCTTCAAG GTGTACGGAG CTGCGTATCT ACAGAAGCTT
    CTGGAGCCGC TGTTGAGAGG AATCATCACT ACACCAGAGT GGCAGCAGAT CAGTTTTGAA
    GTGGACCCTA CTAGACTTGA ACAGGCAGAG AACCTGGAGG AGAACCAGAA GAACCTGCTT
    CAGATCACTG ACCGCTTCTT CCTGGCCATC ATCAACTCCT CGAGCGAGTT TCCTCCACAG
    CTGCGCAGTG TGTGCCACTG TCTGTATCAG GTGGTCAGTC AGCGGTTTCC TCAGAACAGC
    ATTGGTGCTG TAGGAAGTGC CATGTTCCTG CGCTTTATTA ACCCAGCTAT CGTGTCCCCA
    TATGAGGCTG GAATCCTAGA CAAGAAACCC CCACCTCGCA TCGAGCGTGG CCTTAAACTC
    ATGTCTAAGA TTCTGCAGAG CATTGCTAAT CATGTTCTCT TCACAAAAGA GGAGCATATG
    AGGCCCTTTA ATGATTTTGT CAAAAGCAAC TTCGATGCAG CCAGAAGGTT CTTCTTGGAT
    ATCGCATCTG ATTCTCCACC AAGTGATTCT GTGAACCATA GTCTGTCTTT CATCAGTGAC
    GGTAATGTGC TCGCTCTGCA CCGGCTATTA TGGAACAACC AGGAAAGAAT TGGCCAATAC
    CTGTCCAGCA ACAGGGACCA CAAGGCAGTG GGCCGTAGAC CATTTGACAA GATGGCCACT
    CTTCTGGCGT ACCTGGGTCC TCCTGAACAT AAGCCAGTAG CAGACACACA CTGGTCCAGC
    CTCAACCTCA CCAGCTCAAA GTTTGAGGAG TTCATGACAA GGCATCAAGT CCATGAGAAG
    GAGGAATTTA AAGCATTAAA AACCCTCAAC ATATTTTACC AAGCTGGCAC ATCTAAGAAC
    GGCAACCCTG TGTTTTATTA CATCGCACGC AGGTTCAAAA CTGGACAAAT CAATGGTGAT
    CTTCTGATCT ACCACGTCCT GCTGACGCTC AAGCCCTACT ATGCCAAGCA TTATGAGATC
    GTAGTTGACC TTACTCATGT GGGCCCCAGT AACCGTTTCA AGACGGACTT CTTATCCAAA
    TGGTTTGTGG TCTTTCCAAA CTTCGCCTAT GAGAACGTGG CTGCTGTGTA CATCTACAAC
    TGCAACACCT GGGTACGCGA GTACACCAAA TACCACGAGC GACTGCTGAC TGGCCTCAAG
    GGGAGCAAGA AGCTGGTGTT TATCGATTCG CCTGCACGTC TAGCTGAACA CATCGAACCC
    GAACAGCAGA AGCTGCCCGC AGCTACTTTA GCACTGGAAG AAGACCTCAA AGTGTTCCAC
    AACGCCCTAA AGCTTGCACA CAGAGACACA AAAGTGTCAA TTAAGGTGGG ATCCACAGCT
    GTACAGGTGA CCTCGGCCGA AAGAACGCGT GTCCTAGGAC AGCCTGTGTT CCTAAATGAC
    ATCTACTACG CTTCAGAGAT CGAGGAGATA TGCCTGGTGG ACGAGAACCA GTTCACGCTA
    ACCATCGCTA ACCAGGGCAC CCCACTCACT TTCATGCACC AGGAGTGTGA AGCCATCGTT
    CAGTCCATCA TCCACATCCG CACGCGCTGG GAACTTTCCC AACCAGACTC CATTCCCCAA
    CACACCAAAA TCAGGCCTAA GGATGTTCCT GGCACCCTGC TCAACATCGC TCTTCTCAAC
    CTGGGAAGCT CCGACCCCAG TCTGAGGTCT GCGGCCTACA ATCTCCTTTG TGCCTTAACC
    TGCACCTTCA ACCTGAAGAT TGAAGGCCAG CTTCTGGAAA CGTCTGGCTT GTGCATCCCA
    GCCAACAACA CGCTTTTTAT TGTGTCCATT AGCAAAACGC TAGCAGCTAA TGAACCCCAC
    CTCACTCTGG AGTTCCTGGA AGAGTGCATC TCAGGATTCA GTAAATCAAG TATAGAGCTG
    AAGCATCTGT GTCTAGAATA TATGACTCCT TGGCTCCGTA ATTTGGTGCG CTTTTGCAAA
    CACAATGATG ACGCCAAACG GCAGCGTGTC ACGGCCATCC TGGACAAGCT TATTACTATG
    ACCATCAACG AGAAGCAGAT GTACCCCTCC ATACAGGCCA AGATATGGGG CAGCTTGGGT
    CAGATCACAG ACCTGCTTGA TGTGGTGTTG GACAGCTTTA TAAAGACCAG CGCTACTGGC
    GGTTTAGGAT CCATCAAAGC TGAAGTGATG GCTGACACTG CTGTAGCTTT GGCGTCAGGA
    AATGTCAAAC TGGTCTCCAG CAAGGTGATT GGCCGAATGT GTAAGATCAT CGACAAGACG
    TGTCTGTCTC CCACTCCGAC CCTTGAGCAG CACCTCATGT GGGACGACAT CGCCATCCTG
    GCCCGGTACA TGCTCATGCT GTCCTTCAAC AACTCTCTGG ACGTGGCCGC TCATCTGCCA
    TACCTGTTCC ACGTCGTCAC CCTGCTGGTG GCCACGGGCC CTCTCTCGCT GCGTGCATCA
    ACACACGGCC TGGTCATCAA CATCATTCAC TCGCTCTGCA CATGCTCACA ACTCAACTTC
    AGTGAGGAGA CTAAACAGGT GCTGCGCCTG AGTCTGACTG AGTTCTCCCT GCCCAAGTTC
    TACCTGCTGT TCGGCATCAG TAAGGTCAAA TCTGCGGCGG TCATCGCTTT CCGTTCCAGC
    TACAGAGACC GCTCATTCTC TCCAGGCTCT TATGAGAGAG AGACATTTGC TCTGTCTTCC
    CTGGAAACAG TCACGGAGGC TCTTTTGGAG ATCATGGAGG CCTGCATGAG GGACATTCCT
    GGATGCAAAT GGCTAGATCA GTGGACTGAA TTGGCGCAAA AGTTTGCGTT CCAGTATAAC
    CCCTCTCTTC AGCCTCGGGC GCTGGTGGTG TTCGGCTGCA TCAGTAAACG AGTGACTCAC
    GGACAGCTAA AGCAGATCAT TCGTATTCTC AGTAAAGCCA GTCCTCTACT CAGCATTGAT
    CGGGGGCTGG AGAGCTGCTT AAAGGGCCCA GATAATTACA ACAGCCAGGT TCTCATCGAA
    GCCACAGTCA TCGCTCTCAC CAAACTACAG CCTCTCCTCG ATCAGGATTC TCCTATGCAT
    AAAGCTCTGT TCTGGGTGGC CGTGGCCGTG CTTCAGTTGG ATGAAGTGAA TCTGTACTCC
    GCCGGCACTG CCTTACTGGA GCAGAACCTG CACACACTGG ACAGCCTCCG AGTGTTTAAT
    GATAAGAGTC CTGAAGAGGT TTTTATGGAG ATCCGGAAAC CTCTAGAGTG GCACTGTAAA
    CAAATGGACC ATTTTGTGGG ACTCAACTTT GCCTCCAACT TCAATTTTGC ATTAGTGGGC
    CATTTACTTA AAGGATACAG ACATCCCTCT TCAACAACAG TTGCCAGGAC AGTTCGTATC
    CTGCATACAC TCCTGGGCCT GGTGGGAAAA CACCGCAGCT GTGACAAGTT TGAGGTGAAC
    ACGCAGAGTG TGGCCTATCT GGCAGCTTTG CTCACAGTGT CAGAGGAAGT TAGGAGCCGC
    TGCAGTTTAA AGCACAGGAA ATCATTGCTG CTTTCAGAAG TTACAATGGA GAACTTCCCC
    ATGGACACAT ACACCGTTTA CGACACTGAA CCCGGGTGCA GGCTACTGCG TGAAAACCAG
    CCTTGGGCTT CTCCGAAAGT GTCAGAGCGC AGTCTTGCAC CTCCGTACTC CACAATAGGG
    CAGACCAGCC TTCGTCCACG CAAATCCATG AGTGTAGACA TGGGCCAGCC GTCTCAGGCC
    AATGCGAAGA AACTACTGGG TATTAGCGGC ACTAGAAAGA GTTTCGATCA CTTAATATCA
    GAATCCAAAG CCCCAAAAAG ACCAGACATG GAGTCGGGCA TCACCACACC TCCAAAGATG
    AGGCGGGTTT CTGAAAATGA CTATGAGATC GAAACTCAGC GAATAGCCAA CTCTCATTTA
    CGCAAAGTGT CTGTGTCTGA ATCCAATGTC CTCCTGGACG AGAACGTACT TACGGACCCC
    AAGATCCAGA CGTTGCTCCT CACTGTACTG GCAACACTGG TGAAGTACAC TACAGACGAG
    TTTCATCAGC GGATCTTGTA TGAGTATTTG GCTGAGGCCA GTGTGGTCTT CCCCAAGGTC
    TTTCCAGTTG TGCACAACCT GCTGGACTCC AAGTTAAGCA CCGTCCTTTC CATGTGCCAG
    GACCCGAACC TTCTGAACCC CGTCCATGGC ATCATTCAGA ACGTGGTCTA TCATGAGGAG
    TCTCCCCCTC AGTACCAGCC GTCCTGCTTG CAGAGCTTTG GTTTCAATGG TCTTTGGAGG
    TTTGCCGGGC CGTTCTCTAA GCAAACCCAA ATTCCAGACT ACGCCGAGCT GGTGGTTAAA
    TTTTTGGATG CTTTGATTGA CACGTATCTC CCGGGCATTG AGGAGGAAGG AGGAGAAGAG
    TCACTGCGGA CCCCGACCTC GCCATATCCA CCAACCGTCC AGAGCCAGCT GAGCATCACA
    GCCAATCTCA ACCTGTCCAA CTCCATGACA TCACTTGCCA CATCACAGCA CTCCCCAGGC
    ATCGACAAGG AGAACGTTGA GATTTCTCCC ACCTCGAGTC TGTCCACCAG TGGGCGAATG
    CGGCACGGCT CAGCAAGCCA GGTGCAGAAG CAGCGCAGCG CAGGCAGCTT CAAACGGCCA
    AGCATCAAAA AGATCGTCTG A

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa55274
    Clone ID Related Accession (Same CDS sequence) XM_017351197.1
    Accession Version XM_017351197.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 8274bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product neurofibromin isoform X9
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007126.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    ATGGCGGCGC ACAAGCCCGT GGAATGGGTC CAGGCCATCA TTAACAGATT CGACGAACAG 
    CTCCCAATCA AAACAGGACA GCAGAGCACC CACACCAAAG TGAGTACAGA GCACAACAAA
    GAATGTTTGA TCAACATCTC CAAGTACAAA TTTGCCCTCG TCATCAATGG CCTGACCAAC
    ATACTCAAAA ATGTGAACAA CATGCGGATA TTTGGCGAAG CTGCTGAGAA GAACCTCTAC
    TTCTCCCAGC TGATCATACT GGACACACTG GAGAAGTGTC TAGCAGGGCA ACCTAAGGAT
    TGCCTGAGGC TGGATGAGGC TATGTTGGTA AAACAGTTGC TGCCTGAAAT CTGTCACTTC
    CTGCACTCGT ACCGTGAGGG CCAGCAACAC GCCACTCAGC TGCGCAGCTC CGCCTCTGCT
    GTGCTTTTCT CTCTCAGCTG CAACAACTTT AATGCCGTCT TCAGTCGTAT CTCCACCAGG
    TTGCAAGAGC TCACAGTTTG TACAGAGGAC ACAGTGGATG TCCATGACAT TGAGCTCATT
    CAGTATATCA ATGTAGATTG CTCCAAGTTA AAGAGACTCC TACAAGAAGC AGTACTTAAG
    TTCAAGTCTT TGAAAAAGCC GGCTCAGCTT GCAGTAATCA GCAGTTTGGA AAAGGCTTTC
    TGGAACTGGG TTGAACACTA CCCTGATGAT TTCACTATGC TCTATCAGAG ACCCCAGGCC
    GACATGGCCG ACTGTGCAGA GAGACTGTTC GACCTAGTGG ACAGTTTTGC GGAAAGCGCA
    AAGAGAAAAG CTGCTGTGTG GCCACTGCAG ATCATCCTCC TCATCCTCTG TCCAGAGATC
    ACGCAGAGCA TCACCAAAGA AGTAGGGGGA GAGGAGAAAG CTAACAAGAA GTCATTTGTA
    GACAGTTTAC GAAAGGCTCT TGGCCAACAC AGCGGCAGCA AACAATTGAC GGAAAGTGCA
    GCTGTTGCAT GCGTCAAACT CTGCAAAGCC TGCACATATA TCAACTGGGA GGATAATTCT
    GTCATCTTTC TCCTGGTTCA GTCCATCGTC ATGGACCTTA AGGGTCTCCT GTTTAACCCC
    ACCAAACCTT TCTCAAGAGG TACGGGCTGC CAGAGCGCCG ATGTAGATTT GATGATCGAC
    TGCTTTGTGT CATGTTATCG CATCAATCCT CGCAACAACC AGCATTTTAA GGTGTGTCTG
    GCCTCGACTT CTCCTCCTAC CTTCCATTTC GTCCTTGTCA ACTCCCTGCA CAAAATTATC
    ACAAATTCTC ACCTGGACTG GTGGCCGAAG ATTGACGCCA TCTACTGCTA CTCAGGAGAA
    CTACGCACCA TGTTTGCAGA CACACTGGGT CGAGTGATTC AGAGTCTCAG TGCACATCCG
    CCTCTGAGGA TGACATCAAG TTTGACATTC AAAGAGAAAA TGACAACAAG TTTGAAGTTC
    AAGGAAAAGT CCACAGATCT TGATTCGCGT TCTTTGCTGC TGGCACTGGT CAAACTTATC
    CACGCTGACC CCAAACTAAT GCTGCACAAT CCGGGAAAAC CTGGTCAGGA GATTCAGAAC
    AGCACAGCTG AGATCATCAA AGGTCTGGTT CAGCTGGTTC CTCTTACCCA CAGTGCTGAG
    CTTTCCCAAG AGGCCATGGA GGCATTGCTT GTTTTACATC AGCCAGAAAC CATTGAGCTA
    TGGAACCCGG ACGCTCCCAT TGAGACCTTC TGGGACATCA GCTCTCAGGT GTTATTTCTA
    ATTTGTAAGA AGCTGATTGG ACACCAGATG ATCAACAGTA CAGAGATACT CAAGTGGCTC
    AGGGAGATTC TCATATGCAG GAACAAGTTT CTATTAAAGA ACAAGGAGTG TGCTACACAG
    GGCAGTCTGA TCCCCATCAG CAGACAGGCT CAGACCAAGC TGGAGGTGTG TCTGTACACG
    TTCCTGTGGA GTCCTGATGC AGAGGCTGTT TTGGTGGCCA TGTCCTGTTT CAGGCATCTC
    TGTGAGGAGG CCGATATTCG CTGCAGTGTC GATGAGCTGC CTGTTCACAC AGTCCTACCC
    AATTACAACA CCTTCATTGA GCTTGCATCC GTCAGCAATA TGATGGCCAC AGGTCGCAAC
    ACACTACAAA AAAGAGTGAT GGCACTTTTA AGAAGAATAA AGCACCCTAC AGCTGGAAAT
    GTAGAGGCAT GGGAAGACAC ATATGCAAAG TGGGAGCAAG CTACAAAACA GATACTCAAC
    CACCCAAAAA ACAAAGTGGA AGATGGACAG GTAGTTAACA TAAGATTGGT CAATTATTTC
    ATCTTTTTTT CCATTCATGG TCATCTATTT TCTGTTCGTC CTCAGGAGTG GATCAACATG
    ACTGGTTTCT TGTGCGCTCT GGGAGGTGTT TGTCTCCAAC AGCGCAGCAC GCCGAGTCTG
    GGCACCTACA GTCCTCCAAT GGGCCCCCTG ACCGAACGCA AGGGGTCCAT GATTTCCATG
    AGCTCCTGCG AGGTAAACAG CGAAACATCC CTGAATCGCT TTCTGGAGCG TCTGCTGGCT
    CTGATGGTCT GCACACATGA GAAAGTGGGA GTGCAAATTC GCACCAACGT CAAAGACCTG
    GTGGGACTGG AGCTCAGTCC GGCTCTCTAT TCAATGCTCT TCACTAAGCT GCGCAACAGC
    ATCGGACGCT TCTTTGACAC CCAGGGGCCG GTTCCTATTA CAGATGCAAA CACTCTGTTT
    GTAGAGCAGA CCATTGCTAT CATGAAGAAC TTGCTGGACA GTCACACTGA AGGAAGCTCA
    GAGCACCTTG GTCAAGCCAG CATTGAGACC ATGATGCTCA ATCTAGTTAG GTATGTGCGT
    ATTTTAGGGA ACATTGTTCA TGCCATTCAG ATCAAAACCA AGTTGTGTCA GTTAGTGGAG
    GTGATGATGG AGCGCAGGGA TGACCTCTCC TTCTGCCAGG AGATGAAATT CAGGAATAAA
    ATGGTGGAGT ACCTCACAGA CTGGGTGATG GGAACCTCCA ATCAAGCTGC GGACGATGAT
    GTCAAATGCC TGACCCGCGA TCTGGACCAG GCCAGTATGG AGGCTGTCGT GTCTCTGTTG
    GCTGGTCTTC CTCTACAGCC TGAGGAGGGT GACGGTGTTG AGCTCATGGA GGCCAAATCA
    CAGCTCTTCC TGAAGTACTT CACACTGTTC ATGAACCTGT TGAATGACTG CAGCGAGGTT
    GAGGAAGATG GCCAGCAGGT CGCAGGGAGG AAACGTGGCA TGTCTAGACG ACTGGCATCT
    CTGCGGCACT GCACAGTATT GGCTATGTCT AACTTGCTCA ATGCTAATGT GGATAGCGGC
    CTCATGCACT CAATCGGTCT GGGATACCAT AAGGACCTGC AGACACGGGC TACATTTATG
    GAAGTGCTCA CTAAGATCCT TCAGCAAGGG ACGGAGTTCG ACACGCTTGC AGAGACTGTG
    CTTGCAGATC GCTTTGAGAG ACTGGTCGAG CTGGTCACCA TGATGGGAGA TCAGGGAGAG
    CTGCCCATAG CCATGGCTCT GGCCAATGTG GTCCCATGCT CTCAATGGGA TGAGCTGGCC
    AGAGTGTTAG TGACTCTCTT CGACTCTCGC CATCTGCTGT ATCAGCTGTT GTGGAATATG
    TTCTCCAAAG AGGTGGAGCT GGCAGACTCA ATGCAGACGC TCTTTAGAGG AAATAGCCTA
    GCCAGCAAGA TTATGACCTT CTGCTTCAAG GTGTACGGAG CTGCGTATCT ACAGAAGCTT
    CTGGAGCCGC TGTTGAGAGG AATCATCACT ACACCAGAGT GGCAGCAGAT CAGTTTTGAA
    GTGGACCCTA CTAGACTTGA ACAGGCAGAG AACCTGGAGG AGAACCAGAA GAACCTGCTT
    CAGATCACTG ACCGCTTCTT CCTGGCCATC ATCAACTCCT CGAGCGAGTT TCCTCCACAG
    CTGCGCAGTG TGTGCCACTG TCTGTATCAG GTGGTCAGTC AGCGGTTTCC TCAGAACAGC
    ATTGGTGCTG TAGGAAGTGC CATGTTCCTG CGCTTTATTA ACCCAGCTAT CGTGTCCCCA
    TATGAGGCTG GAATCCTAGA CAAGAAACCC CCACCTCGCA TCGAGCGTGG CCTTAAACTC
    ATGTCTAAGA TTCTGCAGAG CATTGCTAAT CATGTTCTCT TCACAAAAGA GGAGCATATG
    AGGCCCTTTA ATGATTTTGT CAAAAGCAAC TTCGATGCAG CCAGAAGGTT CTTCTTGGAT
    ATCGCATCTG ATTCTCCACC AAGTGATTCT GTGAACCATA GTCTGTCTTT CATCAGTGAC
    GGTAATGTGC TCGCTCTGCA CCGGCTATTA TGGAACAACC AGGAAAGAAT TGGCCAATAC
    CTGTCCAGCA ACAGGGACCA CAAGGCAGTG GGCCGTAGAC CATTTGACAA GATGGCCACT
    CTTCTGGCGT ACCTGGGTCC TCCTGAACAT AAGCCAGTAG CAGACACACA CTGGTCCAGC
    CTCAACCTCA CCAGCTCAAA GTTTGAGGAG TTCATGACAA GGCATCAAGT CCATGAGAAG
    GAGGAATTTA AAGCATTAAA AACCCTCAAC ATATTTTACC AAGCTGGCAC ATCTAAGAAC
    GGCAACCCTG TGTTTTATTA CATCGCACGC AGGTTCAAAA CTGGACAAAT CAATGGTGAT
    CTTCTGATCT ACCACGTCCT GCTGACGCTC AAGCCCTACT ATGCCAAGCA TTATGAGATC
    GTAGTTGACC TTACTCATGT GGGCCCCAGT AACCGTTTCA AGACGGACTT CTTATCCAAA
    TGGTTTGTGG TCTTTCCAAA CTTCGCCTAT GAGAACGTGG CTGCTGTGTA CATCTACAAC
    TGCAACACCT GGGTACGCGA GTACACCAAA TACCACGAGC GACTGCTGAC TGGCCTCAAG
    GGGAGCAAGA AGCTGGTGTT TATCGATTCG CCTGCACGTC TAGCTGAACA CATCGAACCC
    GAACAGCAGA AGCTGCCCGC AGCTACTTTA GCACTGGAAG AAGACCTCAA AGTGTTCCAC
    AACGCCCTAA AGCTTGCACA CAGAGACACA AAAGTGTCAA TTAAGGTGGG ATCCACAGCT
    GTACAGGTGA CCTCGGCCGA AAGAACGCGT GTCCTAGGAC AGCCTGTGTT CCTAAATGAC
    ATCTACTACG CTTCAGAGAT CGAGGAGATA TGCCTGGTGG ACGAGAACCA GTTCACGCTA
    ACCATCGCTA ACCAGGGCAC CCCACTCACT TTCATGCACC AGGAGTGTGA AGCCATCGTT
    CAGTCCATCA TCCACATCCG CACGCGCTGG GAACTTTCCC AACCAGACTC CATTCCCCAA
    CACACCAAAA TCAGGCCTAA GGATGTTCCT GGCACCCTGC TCAACATCGC TCTTCTCAAC
    CTGGGAAGCT CCGACCCCAG TCTGAGGTCT GCGGCCTACA ATCTCCTTTG TGCCTTAACC
    TGCACCTTCA ACCTGAAGAT TGAAGGCCAG CTTCTGGAAA CGTCTGGCTT GTGCATCCCA
    GCCAACAACA CGCTTTTTAT TGTGTCCATT AGCAAAACGC TAGCAGCTAA TGAACCCCAC
    CTCACTCTGG AGTTCCTGGA AGAGTGCATC TCAGGATTCA GTAAATCAAG TATAGAGCTG
    AAGCATCTGT GTCTAGAATA TATGACTCCT TGGCTCCGTA ATTTGGTGCG CTTTTGCAAA
    CACAATGATG ACGCCAAACG GCAGCGTGTC ACGGCCATCC TGGACAAGCT TATTACTATG
    ACCATCAACG AGAAGCAGAT GTACCCCTCC ATACAGGCCA AGATATGGGG CAGCTTGGGT
    CAGATCACAG ACCTGCTTGA TGTGGTGTTG GACAGCTTTA TAAAGACCAG CGCTACTGGC
    GGTTTAGGAT CCATCAAAGC TGAAGTGATG GCTGACACTG CTGTAGCTTT GGCGTCAGGA
    AATGTCAAAC TGGTCTCCAG CAAGGTGATT GGCCGAATGT GTAAGATCAT CGACAAGACG
    TGTCTGTCTC CCACTCCGAC CCTTGAGCAG CACCTCATGT GGGACGACAT CGCCATCCTG
    GCCCGGTACA TGCTCATGCT GTCCTTCAAC AACTCTCTGG ACGTGGCCGC TCATCTGCCA
    TACCTGTTCC ACGTCGTCAC CCTGCTGGTG GCCACGGGCC CTCTCTCGCT GCGTGCATCA
    ACACACGGCC TGGTCATCAA CATCATTCAC TCGCTCTGCA CATGCTCACA ACTCAACTTC
    AGTGAGGAGA CTAAACAGGT GCTGCGCCTG AGTCTGACTG AGTTCTCCCT GCCCAAGTTC
    TACCTGCTGT TCGGCATCAG TAAGGTCAAA TCTGCGGCGG TCATCGCTTT CCGTTCCAGC
    TACAGAGACC GCTCATTCTC TCCAGGCTCT TATGAGAGAG AGACATTTGC TCTGTCTTCC
    CTGGAAACAG TCACGGAGGC TCTTTTGGAG ATCATGGAGG CCTGCATGAG GGACATTCCT
    GGATGCAAAT GGCTAGATCA GTGGACTGAA TTGGCGCAAA AGTTTGCGTT CCAGTATAAC
    CCCTCTCTTC AGCCTCGGGC GCTGGTGGTG TTCGGCTGCA TCAGTAAACG AGTGACTCAC
    GGACAGCTAA AGCAGATCAT TCGTATTCTC AGTAAAGCCA GTCCTCTACT CAGCATTGAT
    CGGGGGCTGG AGAGCTGCTT AAAGGGCCCA GATAATTACA ACAGCCAGGT TCTCATCGAA
    GCCACAGTCA TCGCTCTCAC CAAACTACAG CCTCTCCTCG ATCAGGATTC TCCTATGCAT
    AAAGCTCTGT TCTGGGTGGC CGTGGCCGTG CTTCAGTTGG ATGAAGTGAA TCTGTACTCC
    GCCGGCACTG CCTTACTGGA GCAGAACCTG CACACACTGG ACAGCCTCCG AGTGTTTAAT
    GATAAGAGTC CTGAAGAGGT TTTTATGGAG ATCCGGAAAC CTCTAGAGTG GCACTGTAAA
    CAAATGGACC ATTTTGTGGG ACTCAACTTT GCCTCCAACT TCAATTTTGC ATTAGTGGGC
    CATTTACTTA AAGGATACAG ACATCCCTCT TCAACAACAG TTGCCAGGAC AGTTCGTATC
    CTGCATACAC TCCTGGGCCT GGTGGGAAAA CACCGCAGCT GTGACAAGTT TGAGGTGAAC
    ACGCAGAGTG TGGCCTATCT GGCAGCTTTG CTCACAGTGT CAGAGGAAGT TAGGAGCCGC
    TGCAGTTTAA AGCACAGGAA ATCATTGCTG CTTTCAGAAG TTACAATGGA GAACTTCCCC
    ATGGACACAT ACACCGTTTA CGACACTGAA CCCGGGTGCA GGCTACTGCG TGAAAACCAG
    CCTTGGGCTT CTCCGAAAGT GTCAGAGCGC AGTCTTGCAC CTCCGTACTC CACAATAGGG
    CAGACCAGCC TTCGTCCACG CAAATCCATG AGTGTAGACA TGGGCCAGCC GTCTCAGGCC
    AATGCGAAGA AACTACTGGG TATTAGCGGC ACTAGAAAGA GTTTCGATCA CTTAATATCA
    GAATCCAAAG CCCCAAAAAG ACCAGACATG GAGTCGGGCA TCACCACACC TCCAAAGATG
    AGGCGGGTTT CTGAAAATGA CTATGAGATC GAAACTCAGC GAATAGCCAA CTCTCATTTA
    CGCAAAGTGT CTGTGTCTGA ATCCAATGTC CTCCTGGACG AGAACGTACT TACGGACCCC
    AAGATCCAGA CGTTGCTCCT CACTGTACTG GCAACACTGG TGAAGTACAC TACAGACGAG
    TTTCATCAGC GGATCTTGTA TGAGTATTTG GCTGAGGCCA GTGTGGTCTT CCCCAAGGTC
    TTTCCAGTTG TGCACAACCT GCTGGACTCC AAGTTAAGCA CCGTCCTTTC CATGTGCCAG
    GACCCGAACC TTCTGAACCC CGTCCATGGC ATCATTCAGA ACGTGGTCTA TCATGAGGAG
    TCTCCCCCTC AGTACCAGCC GTCCTGCTTG CAGAGCTTTG GTTTCAATGG TCTTTGGAGG
    TTTGCCGGGC CGTTCTCTAA GCAAACCCAA ATTCCAGACT ACGCCGAGCT GGTGGTTAAA
    TTTTTGGATG CTTTGATTGA CACGTATCTC CCGGGCATTG AGGAGGAAGG AGGAGAAGAG
    TCACTGCGGA CCCCGACCTC GCCATATCCA CCAACCGTCC AGAGCCAGCT GAGCATCACA
    GCCAATCTCA ACCTGTCCAA CTCCATGACA TCACTTGCCA CATCACAGCA CTCCCCAGAG
    GGCCCTATAG TTTATAGACG GTCCCCAACT CTCTCCCATC CACGAACTAC AGAGGAGGTG
    CCACCGAAAA CAGCTGGTAC AAACCTAAAC TATAGAGCCG TGTGGGGCAA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017206686.1
    CDS253..8526
    Translation

    Target ORF information:

    RefSeq Version XM_017351197.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio neurofibromin 1a (nf1a), transcript variant X9, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017351197.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    ATGGCGGCGC ACAAGCCCGT GGAATGGGTC CAGGCCATCA TTAACAGATT CGACGAACAG 
    CTCCCAATCA AAACAGGACA GCAGAGCACC CACACCAAAG TGAGTACAGA GCACAACAAA
    GAATGTTTGA TCAACATCTC CAAGTACAAA TTTGCCCTCG TCATCAATGG CCTGACCAAC
    ATACTCAAAA ATGTGAACAA CATGCGGATA TTTGGCGAAG CTGCTGAGAA GAACCTCTAC
    TTCTCCCAGC TGATCATACT GGACACACTG GAGAAGTGTC TAGCAGGGCA ACCTAAGGAT
    TGCCTGAGGC TGGATGAGGC TATGTTGGTA AAACAGTTGC TGCCTGAAAT CTGTCACTTC
    CTGCACTCGT ACCGTGAGGG CCAGCAACAC GCCACTCAGC TGCGCAGCTC CGCCTCTGCT
    GTGCTTTTCT CTCTCAGCTG CAACAACTTT AATGCCGTCT TCAGTCGTAT CTCCACCAGG
    TTGCAAGAGC TCACAGTTTG TACAGAGGAC ACAGTGGATG TCCATGACAT TGAGCTCATT
    CAGTATATCA ATGTAGATTG CTCCAAGTTA AAGAGACTCC TACAAGAAGC AGTACTTAAG
    TTCAAGTCTT TGAAAAAGCC GGCTCAGCTT GCAGTAATCA GCAGTTTGGA AAAGGCTTTC
    TGGAACTGGG TTGAACACTA CCCTGATGAT TTCACTATGC TCTATCAGAG ACCCCAGGCC
    GACATGGCCG ACTGTGCAGA GAGACTGTTC GACCTAGTGG ACAGTTTTGC GGAAAGCGCA
    AAGAGAAAAG CTGCTGTGTG GCCACTGCAG ATCATCCTCC TCATCCTCTG TCCAGAGATC
    ACGCAGAGCA TCACCAAAGA AGTAGGGGGA GAGGAGAAAG CTAACAAGAA GTCATTTGTA
    GACAGTTTAC GAAAGGCTCT TGGCCAACAC AGCGGCAGCA AACAATTGAC GGAAAGTGCA
    GCTGTTGCAT GCGTCAAACT CTGCAAAGCC TGCACATATA TCAACTGGGA GGATAATTCT
    GTCATCTTTC TCCTGGTTCA GTCCATCGTC ATGGACCTTA AGGGTCTCCT GTTTAACCCC
    ACCAAACCTT TCTCAAGAGG TACGGGCTGC CAGAGCGCCG ATGTAGATTT GATGATCGAC
    TGCTTTGTGT CATGTTATCG CATCAATCCT CGCAACAACC AGCATTTTAA GGTGTGTCTG
    GCCTCGACTT CTCCTCCTAC CTTCCATTTC GTCCTTGTCA ACTCCCTGCA CAAAATTATC
    ACAAATTCTC ACCTGGACTG GTGGCCGAAG ATTGACGCCA TCTACTGCTA CTCAGGAGAA
    CTACGCACCA TGTTTGCAGA CACACTGGGT CGAGTGATTC AGAGTCTCAG TGCACATCCG
    CCTCTGAGGA TGACATCAAG TTTGACATTC AAAGAGAAAA TGACAACAAG TTTGAAGTTC
    AAGGAAAAGT CCACAGATCT TGATTCGCGT TCTTTGCTGC TGGCACTGGT CAAACTTATC
    CACGCTGACC CCAAACTAAT GCTGCACAAT CCGGGAAAAC CTGGTCAGGA GATTCAGAAC
    AGCACAGCTG AGATCATCAA AGGTCTGGTT CAGCTGGTTC CTCTTACCCA CAGTGCTGAG
    CTTTCCCAAG AGGCCATGGA GGCATTGCTT GTTTTACATC AGCCAGAAAC CATTGAGCTA
    TGGAACCCGG ACGCTCCCAT TGAGACCTTC TGGGACATCA GCTCTCAGGT GTTATTTCTA
    ATTTGTAAGA AGCTGATTGG ACACCAGATG ATCAACAGTA CAGAGATACT CAAGTGGCTC
    AGGGAGATTC TCATATGCAG GAACAAGTTT CTATTAAAGA ACAAGGAGTG TGCTACACAG
    GGCAGTCTGA TCCCCATCAG CAGACAGGCT CAGACCAAGC TGGAGGTGTG TCTGTACACG
    TTCCTGTGGA GTCCTGATGC AGAGGCTGTT TTGGTGGCCA TGTCCTGTTT CAGGCATCTC
    TGTGAGGAGG CCGATATTCG CTGCAGTGTC GATGAGCTGC CTGTTCACAC AGTCCTACCC
    AATTACAACA CCTTCATTGA GCTTGCATCC GTCAGCAATA TGATGGCCAC AGGTCGCAAC
    ACACTACAAA AAAGAGTGAT GGCACTTTTA AGAAGAATAA AGCACCCTAC AGCTGGAAAT
    GTAGAGGCAT GGGAAGACAC ATATGCAAAG TGGGAGCAAG CTACAAAACA GATACTCAAC
    CACCCAAAAA ACAAAGTGGA AGATGGACAG GTAGTTAACA TAAGATTGGT CAATTATTTC
    ATCTTTTTTT CCATTCATGG TCATCTATTT TCTGTTCGTC CTCAGGAGTG GATCAACATG
    ACTGGTTTCT TGTGCGCTCT GGGAGGTGTT TGTCTCCAAC AGCGCAGCAC GCCGAGTCTG
    GGCACCTACA GTCCTCCAAT GGGCCCCCTG ACCGAACGCA AGGGGTCCAT GATTTCCATG
    AGCTCCTGCG AGGTAAACAG CGAAACATCC CTGAATCGCT TTCTGGAGCG TCTGCTGGCT
    CTGATGGTCT GCACACATGA GAAAGTGGGA GTGCAAATTC GCACCAACGT CAAAGACCTG
    GTGGGACTGG AGCTCAGTCC GGCTCTCTAT TCAATGCTCT TCACTAAGCT GCGCAACAGC
    ATCGGACGCT TCTTTGACAC CCAGGGGCCG GTTCCTATTA CAGATGCAAA CACTCTGTTT
    GTAGAGCAGA CCATTGCTAT CATGAAGAAC TTGCTGGACA GTCACACTGA AGGAAGCTCA
    GAGCACCTTG GTCAAGCCAG CATTGAGACC ATGATGCTCA ATCTAGTTAG GTATGTGCGT
    ATTTTAGGGA ACATTGTTCA TGCCATTCAG ATCAAAACCA AGTTGTGTCA GTTAGTGGAG
    GTGATGATGG AGCGCAGGGA TGACCTCTCC TTCTGCCAGG AGATGAAATT CAGGAATAAA
    ATGGTGGAGT ACCTCACAGA CTGGGTGATG GGAACCTCCA ATCAAGCTGC GGACGATGAT
    GTCAAATGCC TGACCCGCGA TCTGGACCAG GCCAGTATGG AGGCTGTCGT GTCTCTGTTG
    GCTGGTCTTC CTCTACAGCC TGAGGAGGGT GACGGTGTTG AGCTCATGGA GGCCAAATCA
    CAGCTCTTCC TGAAGTACTT CACACTGTTC ATGAACCTGT TGAATGACTG CAGCGAGGTT
    GAGGAAGATG GCCAGCAGGT CGCAGGGAGG AAACGTGGCA TGTCTAGACG ACTGGCATCT
    CTGCGGCACT GCACAGTATT GGCTATGTCT AACTTGCTCA ATGCTAATGT GGATAGCGGC
    CTCATGCACT CAATCGGTCT GGGATACCAT AAGGACCTGC AGACACGGGC TACATTTATG
    GAAGTGCTCA CTAAGATCCT TCAGCAAGGG ACGGAGTTCG ACACGCTTGC AGAGACTGTG
    CTTGCAGATC GCTTTGAGAG ACTGGTCGAG CTGGTCACCA TGATGGGAGA TCAGGGAGAG
    CTGCCCATAG CCATGGCTCT GGCCAATGTG GTCCCATGCT CTCAATGGGA TGAGCTGGCC
    AGAGTGTTAG TGACTCTCTT CGACTCTCGC CATCTGCTGT ATCAGCTGTT GTGGAATATG
    TTCTCCAAAG AGGTGGAGCT GGCAGACTCA ATGCAGACGC TCTTTAGAGG AAATAGCCTA
    GCCAGCAAGA TTATGACCTT CTGCTTCAAG GTGTACGGAG CTGCGTATCT ACAGAAGCTT
    CTGGAGCCGC TGTTGAGAGG AATCATCACT ACACCAGAGT GGCAGCAGAT CAGTTTTGAA
    GTGGACCCTA CTAGACTTGA ACAGGCAGAG AACCTGGAGG AGAACCAGAA GAACCTGCTT
    CAGATCACTG ACCGCTTCTT CCTGGCCATC ATCAACTCCT CGAGCGAGTT TCCTCCACAG
    CTGCGCAGTG TGTGCCACTG TCTGTATCAG GTGGTCAGTC AGCGGTTTCC TCAGAACAGC
    ATTGGTGCTG TAGGAAGTGC CATGTTCCTG CGCTTTATTA ACCCAGCTAT CGTGTCCCCA
    TATGAGGCTG GAATCCTAGA CAAGAAACCC CCACCTCGCA TCGAGCGTGG CCTTAAACTC
    ATGTCTAAGA TTCTGCAGAG CATTGCTAAT CATGTTCTCT TCACAAAAGA GGAGCATATG
    AGGCCCTTTA ATGATTTTGT CAAAAGCAAC TTCGATGCAG CCAGAAGGTT CTTCTTGGAT
    ATCGCATCTG ATTCTCCACC AAGTGATTCT GTGAACCATA GTCTGTCTTT CATCAGTGAC
    GGTAATGTGC TCGCTCTGCA CCGGCTATTA TGGAACAACC AGGAAAGAAT TGGCCAATAC
    CTGTCCAGCA ACAGGGACCA CAAGGCAGTG GGCCGTAGAC CATTTGACAA GATGGCCACT
    CTTCTGGCGT ACCTGGGTCC TCCTGAACAT AAGCCAGTAG CAGACACACA CTGGTCCAGC
    CTCAACCTCA CCAGCTCAAA GTTTGAGGAG TTCATGACAA GGCATCAAGT CCATGAGAAG
    GAGGAATTTA AAGCATTAAA AACCCTCAAC ATATTTTACC AAGCTGGCAC ATCTAAGAAC
    GGCAACCCTG TGTTTTATTA CATCGCACGC AGGTTCAAAA CTGGACAAAT CAATGGTGAT
    CTTCTGATCT ACCACGTCCT GCTGACGCTC AAGCCCTACT ATGCCAAGCA TTATGAGATC
    GTAGTTGACC TTACTCATGT GGGCCCCAGT AACCGTTTCA AGACGGACTT CTTATCCAAA
    TGGTTTGTGG TCTTTCCAAA CTTCGCCTAT GAGAACGTGG CTGCTGTGTA CATCTACAAC
    TGCAACACCT GGGTACGCGA GTACACCAAA TACCACGAGC GACTGCTGAC TGGCCTCAAG
    GGGAGCAAGA AGCTGGTGTT TATCGATTCG CCTGCACGTC TAGCTGAACA CATCGAACCC
    GAACAGCAGA AGCTGCCCGC AGCTACTTTA GCACTGGAAG AAGACCTCAA AGTGTTCCAC
    AACGCCCTAA AGCTTGCACA CAGAGACACA AAAGTGTCAA TTAAGGTGGG ATCCACAGCT
    GTACAGGTGA CCTCGGCCGA AAGAACGCGT GTCCTAGGAC AGCCTGTGTT CCTAAATGAC
    ATCTACTACG CTTCAGAGAT CGAGGAGATA TGCCTGGTGG ACGAGAACCA GTTCACGCTA
    ACCATCGCTA ACCAGGGCAC CCCACTCACT TTCATGCACC AGGAGTGTGA AGCCATCGTT
    CAGTCCATCA TCCACATCCG CACGCGCTGG GAACTTTCCC AACCAGACTC CATTCCCCAA
    CACACCAAAA TCAGGCCTAA GGATGTTCCT GGCACCCTGC TCAACATCGC TCTTCTCAAC
    CTGGGAAGCT CCGACCCCAG TCTGAGGTCT GCGGCCTACA ATCTCCTTTG TGCCTTAACC
    TGCACCTTCA ACCTGAAGAT TGAAGGCCAG CTTCTGGAAA CGTCTGGCTT GTGCATCCCA
    GCCAACAACA CGCTTTTTAT TGTGTCCATT AGCAAAACGC TAGCAGCTAA TGAACCCCAC
    CTCACTCTGG AGTTCCTGGA AGAGTGCATC TCAGGATTCA GTAAATCAAG TATAGAGCTG
    AAGCATCTGT GTCTAGAATA TATGACTCCT TGGCTCCGTA ATTTGGTGCG CTTTTGCAAA
    CACAATGATG ACGCCAAACG GCAGCGTGTC ACGGCCATCC TGGACAAGCT TATTACTATG
    ACCATCAACG AGAAGCAGAT GTACCCCTCC ATACAGGCCA AGATATGGGG CAGCTTGGGT
    CAGATCACAG ACCTGCTTGA TGTGGTGTTG GACAGCTTTA TAAAGACCAG CGCTACTGGC
    GGTTTAGGAT CCATCAAAGC TGAAGTGATG GCTGACACTG CTGTAGCTTT GGCGTCAGGA
    AATGTCAAAC TGGTCTCCAG CAAGGTGATT GGCCGAATGT GTAAGATCAT CGACAAGACG
    TGTCTGTCTC CCACTCCGAC CCTTGAGCAG CACCTCATGT GGGACGACAT CGCCATCCTG
    GCCCGGTACA TGCTCATGCT GTCCTTCAAC AACTCTCTGG ACGTGGCCGC TCATCTGCCA
    TACCTGTTCC ACGTCGTCAC CCTGCTGGTG GCCACGGGCC CTCTCTCGCT GCGTGCATCA
    ACACACGGCC TGGTCATCAA CATCATTCAC TCGCTCTGCA CATGCTCACA ACTCAACTTC
    AGTGAGGAGA CTAAACAGGT GCTGCGCCTG AGTCTGACTG AGTTCTCCCT GCCCAAGTTC
    TACCTGCTGT TCGGCATCAG TAAGGTCAAA TCTGCGGCGG TCATCGCTTT CCGTTCCAGC
    TACAGAGACC GCTCATTCTC TCCAGGCTCT TATGAGAGAG AGACATTTGC TCTGTCTTCC
    CTGGAAACAG TCACGGAGGC TCTTTTGGAG ATCATGGAGG CCTGCATGAG GGACATTCCT
    GGATGCAAAT GGCTAGATCA GTGGACTGAA TTGGCGCAAA AGTTTGCGTT CCAGTATAAC
    CCCTCTCTTC AGCCTCGGGC GCTGGTGGTG TTCGGCTGCA TCAGTAAACG AGTGACTCAC
    GGACAGCTAA AGCAGATCAT TCGTATTCTC AGTAAAGCCA GTCCTCTACT CAGCATTGAT
    CGGGGGCTGG AGAGCTGCTT AAAGGGCCCA GATAATTACA ACAGCCAGGT TCTCATCGAA
    GCCACAGTCA TCGCTCTCAC CAAACTACAG CCTCTCCTCG ATCAGGATTC TCCTATGCAT
    AAAGCTCTGT TCTGGGTGGC CGTGGCCGTG CTTCAGTTGG ATGAAGTGAA TCTGTACTCC
    GCCGGCACTG CCTTACTGGA GCAGAACCTG CACACACTGG ACAGCCTCCG AGTGTTTAAT
    GATAAGAGTC CTGAAGAGGT TTTTATGGAG ATCCGGAAAC CTCTAGAGTG GCACTGTAAA
    CAAATGGACC ATTTTGTGGG ACTCAACTTT GCCTCCAACT TCAATTTTGC ATTAGTGGGC
    CATTTACTTA AAGGATACAG ACATCCCTCT TCAACAACAG TTGCCAGGAC AGTTCGTATC
    CTGCATACAC TCCTGGGCCT GGTGGGAAAA CACCGCAGCT GTGACAAGTT TGAGGTGAAC
    ACGCAGAGTG TGGCCTATCT GGCAGCTTTG CTCACAGTGT CAGAGGAAGT TAGGAGCCGC
    TGCAGTTTAA AGCACAGGAA ATCATTGCTG CTTTCAGAAG TTACAATGGA GAACTTCCCC
    ATGGACACAT ACACCGTTTA CGACACTGAA CCCGGGTGCA GGCTACTGCG TGAAAACCAG
    CCTTGGGCTT CTCCGAAAGT GTCAGAGCGC AGTCTTGCAC CTCCGTACTC CACAATAGGG
    CAGACCAGCC TTCGTCCACG CAAATCCATG AGTGTAGACA TGGGCCAGCC GTCTCAGGCC
    AATGCGAAGA AACTACTGGG TATTAGCGGC ACTAGAAAGA GTTTCGATCA CTTAATATCA
    GAATCCAAAG CCCCAAAAAG ACCAGACATG GAGTCGGGCA TCACCACACC TCCAAAGATG
    AGGCGGGTTT CTGAAAATGA CTATGAGATC GAAACTCAGC GAATAGCCAA CTCTCATTTA
    CGCAAAGTGT CTGTGTCTGA ATCCAATGTC CTCCTGGACG AGAACGTACT TACGGACCCC
    AAGATCCAGA CGTTGCTCCT CACTGTACTG GCAACACTGG TGAAGTACAC TACAGACGAG
    TTTCATCAGC GGATCTTGTA TGAGTATTTG GCTGAGGCCA GTGTGGTCTT CCCCAAGGTC
    TTTCCAGTTG TGCACAACCT GCTGGACTCC AAGTTAAGCA CCGTCCTTTC CATGTGCCAG
    GACCCGAACC TTCTGAACCC CGTCCATGGC ATCATTCAGA ACGTGGTCTA TCATGAGGAG
    TCTCCCCCTC AGTACCAGCC GTCCTGCTTG CAGAGCTTTG GTTTCAATGG TCTTTGGAGG
    TTTGCCGGGC CGTTCTCTAA GCAAACCCAA ATTCCAGACT ACGCCGAGCT GGTGGTTAAA
    TTTTTGGATG CTTTGATTGA CACGTATCTC CCGGGCATTG AGGAGGAAGG AGGAGAAGAG
    TCACTGCGGA CCCCGACCTC GCCATATCCA CCAACCGTCC AGAGCCAGCT GAGCATCACA
    GCCAATCTCA ACCTGTCCAA CTCCATGACA TCACTTGCCA CATCACAGCA CTCCCCAGAG
    GGCCCTATAG TTTATAGACG GTCCCCAACT CTCTCCCATC CACGAACTAC AGAGGAGGTG
    CCACCGAAAA CAGCTGGTAC AAACCTAAAC TATAGAGCCG TGTGGGGCAA TTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.