rock2b gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_009294695.2	XP_009292970.1	rho-associated, coiled-coil containing protein kinase 2b isoform X4
XM_017352435.1	XP_017207924.1	rho-associated, coiled-coil containing protein kinase 2b isoform X5
XM_009294695.3	XP_009292970.1	rho-associated, coiled-coil containing protein kinase 2b isoform X7
XM_021468360.1	XP_021324035.1	rho-associated, coiled-coil containing protein kinase 2b isoform X6
XM_017352432.1	XP_017207921.1	rho-associated, coiled-coil containing protein kinase 2b isoform X1
XM_009294697.2	XP_009292972.1	rho-associated, coiled-coil containing protein kinase 2b isoform X8
XM_017352434.2	XP_017207923.1	rho-associated, coiled-coil containing protein kinase 2b isoform X5
XM_017352437.2	XP_017207926.1	rho-associated, coiled-coil containing protein kinase 2b isoform X9
XM_017352433.1	XP_017207922.1	rho-associated, coiled-coil containing protein kinase 2b isoform X2
XM_017352436.1	XP_017207925.1	rho-associated, coiled-coil containing protein kinase 2b isoform X6
XM_021468356.1	XP_021324031.1	rho-associated, coiled-coil containing protein kinase 2b isoform X1
XM_021468359.1	XP_021324034.1	rho-associated, coiled-coil containing protein kinase 2b isoform X3
XM_017352434.1	XP_017207923.1	rho-associated, coiled-coil containing protein kinase 2b isoform X3
XM_017352437.1	XP_017207926.1	rho-associated, coiled-coil containing protein kinase 2b isoform X7
NM_001093747.2	NP_001087216.1	rho-associated, coiled-coil containing protein kinase 2b
XM_021468357.1	XP_021324032.1	rho-associated, coiled-coil containing protein kinase 2b isoform X2
XM_021468358.1	XP_021324033.1	rho-associated, coiled-coil containing protein kinase 2b isoform X2
XM_017352433.2	XP_017207922.1	rho-associated, coiled-coil containing protein kinase 2b isoform X4
XM_021468361.1	XP_021324036.1	rho-associated, coiled-coil containing protein kinase 2b isoform X8
XM_009294697.3	XP_009292972.1	rho-associated, coiled-coil containing protein kinase 2b isoform X10

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa39642	XM_009294697.3 Latest version!	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa24919	XM_017352436.1 Latest version!	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	15-17	$629.30 $899.00
ODa150578	XM_021468357.1 Latest version!	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa150578	XM_021468358.1 Latest version!	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa150579	XM_021468359.1 Latest version!	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa56542	XM_017352435.1 Latest version!	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
ODa24920	XM_017352437.2 Latest version!	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa00028	NM_001093747.2 Latest version!	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa24918	XM_009294695.3 Latest version!	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa39639	XM_021468356.1 Latest version!	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa39639	XM_017352432.1 Latest version!	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa39640	XM_017352433.2 Latest version!	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa39641	XM_017352434.2 Latest version!	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa150580	XM_021468360.1 Latest version!	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa150581	XM_021468361.1 Latest version!	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
View Old Accession Versions >>
ODa24918	XM_009294695.2	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa39641	XM_017352434.1	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa24920	XM_017352437.1	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa39640	XM_017352433.1	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$797.30 $1139.00
ODa39642	XM_009294697.2	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

rock2b ( XM_009294697.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292972.1 Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X8, mRNA.

Price & Availability↑

CloneID	ODa39642
Clone ID Related Accession (Same CDS sequence)	XM_009294697.2 , XM_009294697.3
Accession Version	XM_009294697.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2826bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	rho-associated, coiled-coil containing protein kinase 2b isoform X8
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294697.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCAGTG GACCTTCGAC ACCATACGAG ACACTGGAGG AGTCGGAGAG GCTGAAGAAG 
GCCCAGATCG AGGCCTTTAG ACAGTCCCAG CAGCTGGAGC TTTCCCTCAG GGAGCTGCAG 
GAGCGTCTGG CCCAGCTGGA GAACAGCAGG CTGGTTCTGG AGCAGGACAA ACTCAGCCTG 
CAGACCTCGC TAGAGCTGGA GAAACGAGAG AGAAACGCCG GCTCAGAGAC CATCACTGAC 
CTGCAGGGTC GTATCTATGG ACTGGAGGGT GAGTTGAAGC ATATAAAGAG TTCTCTATCA 
AAAGCTGAAG TTGAGAAAAG ACAATTACAA GAGGATCTTA CAACCCTGGA AAAGGAGAAG 
AATAAGCAGG AGATCGACCT CTCCTTCAAA CTCAAAGCAG TTCAGCAGAG TCTGGAGCAG 
GAGGAGGCTG AACATAAAAC CACTAAAGCC AGATTGGCCG ACAACAACAA AATCAACCAA 
TCCATAGAGG CAAAGTCTGA GACCCTGAAA GACATGGAAC ATAAGCTGTT GGAAGAACGA 
TCTGCGAAGC AACAACTGGA AAACAGACTG ATGCAGCTGG AGAAGGAAAA CTCTGTATTA 
GACTGTGATT ACAAACAAGC CAAACATGAG CTTCAGGAGC TCCGCTCGCT CAAAGAAAAT 
CTCACAGAGC AGGTGGAGGT GCTGAATGTG CGGGTGCAGC AGGAGACACA GAGGAAAACT 
CTCTGTCAAG GTGATCTCAA AGTTCAGAGG CAGGAGATCA ACAGCCTGCG GTCATCAGAA 
CAACAGCTCA AACAAGAACT CAACCATCTG CTGGAGCTCA AACTCACACT GGAGAAACAA 
AACCAGGAGC TGTCCAAGGA ACGAGAAGAA TCTGAAAAAC AGCTGAAGGA GATGAAGGAC 
CAGCTGGAGG CTGAGCAGTA CTTCACGAAA CTGTACAAGA CTCAGATTCG AGAGCTGAAA 
GAGGAAAGCG ATGAAAAGGT TAAACTCTAC AAAGATGCTC AGCAGAGAAT AGAGGACCTG 
CAGGAAGAGA GGGACTCGTT AGCGTCTCAG TTGGAGGTGA GCCTGACGAA GGCTGACTCT 
GAACAGTTGG CTCGCATCAC CGTTGAAGAG CAATACTCGG ACCTGGAGAA AGAGAAGATC 
ATGAAGGAAC TGGAGATAAA GGACATGATC GCCAGACACC GGCAGGATCT TGCAGAAAAG 
GACGGTACCA TCAACTCGCT GGAGGAGTCA AACCGTACGC TGACTGTGGA TGTTGCCAAC 
CTGGCCAGTG AGAAAGAGGA GCTCAACAAT AAACTGAAGC ACATACAGCA AAAGCTGGAG 
AAGATCAAAG AAGAAGAGAA ACAGATGAAA TCTCTCACAG TGTCGTATGA GAAGCAGATA 
CAGGTGGAGA AAACACTCAA GATTCAGGCT ATAAACAAGC TGGCGGAGGT CATGAAGAAG 
ACGGACGGCA GGCCACACCA AATCAACAAC ACAGACATCC GCAGGAAGGA GAAGGAGATC 
AGACAACTCC AGCTGCAGCT CCGCACGGAG AAAGAAAAGC TTAACCACTC CATCATCAAA 
TACCAGAGAG AAATCAACGA CATGCAGGCT ATGATAGCAG AGGAGTCTCA GGTGCGTCTG 
GAGATGCAGA TGTCTCTGGA CAGTAAAGAC AGTGATATTG AGCGTCTGCG CAGTCAACTC 
ACGTCTCTGA GCATTCACTC TCTGGACACC ACCAGCATCA GCAGCATCGG CAATGACCTG 
GATTCTGACG AGGCATATCC AGTGCGAATC ACACACTCAC ACACCTCGGA GTCTATGTCC 
TTCACATACC AGCGAAGCTC CAAGTCGGTG TGTATTGATA CCCGGCCCTG TCGAATCGCT 
CCTCGCTTCT CCTCTGACTC TGATGAAGAG GAGGATGAGG AAGATGAGTG GAGACAAACA 
CCTGCCCTTA CATATGAGAC GCCTCCTGAC CCTGAGGCTG CAGCCGCAGA GTCCGGTCTT 
GAAGGATGGC TCTCAATGCC TGCCAAAAAC ACCAAACGGT TTGGATGGGA TAAGAAGTAT 
GTGGTTGTGA GCAGCAAGAA AATCCTGTTT TATGACTCTG AACAAGACCG AGAGCAGTCC 
AGTCCATTCA TGATCCTGGA CATTGACAAA CTCTTCCACG TGAGACCCGT CACTCAGACA 
GATGTTTACC GCGCAGATCC CAGAGAAATC CCTCGCATCT TCCAGATACT ATATGCGAAT 
GAAGGAGAGA GCAAGAAAGA GGAGCTGCCC GTGGAGTCGT TATCTGTGAC TGAGAGGTCA 
CTGTGTATCC CACACAAGGG TCACGAGTTT GTTCTGACGC TTTATCACTT CCCCTCGAGC 
TGCGAGGTCT GTCCACGACC CTTATGGCAC GTCTTCAGAC CTCCGCCGGC TCTCGAGTGC 
AGACGCTGTA GAGTCAAGTG CCATAAAGAC CACATCGATC GCAAAGAAGA GGTCCTCGCT 
CCATGTAGAG TGAATTACGA CATGTCCACA GCGAAAGAGA TGCTGCTGCT GGCAAACTCC 
ACAGAGGAGC AGAAGAAGTG GGTCAGTCGG CTGCTCAAGA GGGTTCCGCG TAAACCTTCC 
ATCGCCCACT CTTCCAGCAT AGCCATATCC GCTGCGCCCT CAGAGGCAAC ACCCACTACC 
CTTTCCCCTC AGCCGTCGCC TCGCCTCGCT CAGTCTTCAC CCAGACTTTC ACACCGAGCA 
GCCGTCAAAG TGCACTCTAC ACGACAGCAG CCCTCCAGCA AGAACAGATT GCTTGAAATG 
GGCCTGAAGG ACTGGGGTTG GGCTCTTGAG GTTGAATTGG ATGATGACGA TGTTTTAGAC 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292972.1
CDS	132..2957
Translation	MISGPSTPYETLEESERLKKAQIEAFRQSQQLELSLRELQERLAQLENSRLVLEQDKLSLQTSLELEKRERNAGSETITDLQGRIYGLEGELKHIKSSLSKAEVEKRQLQEDLTTLEKEKNKQEIDLSFKLKAVQQSLEQEEAEHKTTKARLADNNKINQSIEAKSETLKDMEHKLLEERSAKQQLENRLMQLEKENSVLDCDYKQAKHELQELRSLKENLTEQVEVLNVRVQQETQRKTLCQGDLKVQRQEINSLRSSEQQLKQELNHLLELKLTLEKQNQELSKEREESEKQLKEMKDQLEAEQYFTKLYKTQIRELKEESDEKVKLYKDAQQRIEDLQEERDSLASQLEVSLTKADSEQLARITVEEQYSDLEKEKIMKELEIKDMIARHRQDLAEKDGTINSLEESNRTLTVDVANLASEKEELNNKLKHIQQKLEKIKEEEKQMKSLTVSYEKQIQVEKTLKIQAINKLAEVMKKTDGRPHQINNTDIRRKEKEIRQLQLQLRTEKEKLNHSIIKYQREINDMQAMIAEESQVRLEMQMSLDSKDSDIERLRSQLTSLSIHSLDTTSISSIGNDLDSDEAYPVRITHSHTSESMSFTYQRSSKSVCIDTRPCRIAPRFSSDSDEEEDEEDEWRQTPALTYETPPDPEAAAAESGLEGWLSMPAKNTKRFGWDKKYVVVSSKKILFYDSEQDREQSSPFMILDIDKLFHVRPVTQTDVYRADPREIPRIFQILYANEGESKKEELPVESLSVTERSLCIPHKGHEFVLTLYHFPSSCEVCPRPLWHVFRPPPALECRRCRVKCHKDHIDRKEEVLAPCRVNYDMSTAKEMLLLANSTEEQKKWVSRLLKRVPRKPSIAHSSSIAISAAPSEATPTTLSPQPSPRLAQSSPRLSHRAAVKVHSTRQQPSSKNRLLEMGLKDWGWALEVELDDDDVLDF

Target ORF information:

RefSeq Version	XM_009294697.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294697.2

ORF Insert Sequence:

ATGATCAGTG GACCTTCGAC ACCATACGAG ACACTGGAGG AGTCGGAGAG GCTGAAGAAG 
GCCCAGATCG AGGCCTTTAG ACAGTCCCAG CAGCTGGAGC TTTCCCTCAG GGAGCTGCAG 
GAGCGTCTGG CCCAGCTGGA GAACAGCAGG CTGGTTCTGG AGCAGGACAA ACTCAGCCTG 
CAGACCTCGC TAGAGCTGGA GAAACGAGAG AGAAACGCCG GCTCAGAGAC CATCACTGAC 
CTGCAGGGTC GTATCTATGG ACTGGAGGGT GAGTTGAAGC ATATAAAGAG TTCTCTATCA 
AAAGCTGAAG TTGAGAAAAG ACAATTACAA GAGGATCTTA CAACCCTGGA AAAGGAGAAG 
AATAAGCAGG AGATCGACCT CTCCTTCAAA CTCAAAGCAG TTCAGCAGAG TCTGGAGCAG 
GAGGAGGCTG AACATAAAAC CACTAAAGCC AGATTGGCCG ACAACAACAA AATCAACCAA 
TCCATAGAGG CAAAGTCTGA GACCCTGAAA GACATGGAAC ATAAGCTGTT GGAAGAACGA 
TCTGCGAAGC AACAACTGGA AAACAGACTG ATGCAGCTGG AGAAGGAAAA CTCTGTATTA 
GACTGTGATT ACAAACAAGC CAAACATGAG CTTCAGGAGC TCCGCTCGCT CAAAGAAAAT 
CTCACAGAGC AGGTGGAGGT GCTGAATGTG CGGGTGCAGC AGGAGACACA GAGGAAAACT 
CTCTGTCAAG GTGATCTCAA AGTTCAGAGG CAGGAGATCA ACAGCCTGCG GTCATCAGAA 
CAACAGCTCA AACAAGAACT CAACCATCTG CTGGAGCTCA AACTCACACT GGAGAAACAA 
AACCAGGAGC TGTCCAAGGA ACGAGAAGAA TCTGAAAAAC AGCTGAAGGA GATGAAGGAC 
CAGCTGGAGG CTGAGCAGTA CTTCACGAAA CTGTACAAGA CTCAGATTCG AGAGCTGAAA 
GAGGAAAGCG ATGAAAAGGT TAAACTCTAC AAAGATGCTC AGCAGAGAAT AGAGGACCTG 
CAGGAAGAGA GGGACTCGTT AGCGTCTCAG TTGGAGGTGA GCCTGACGAA GGCTGACTCT 
GAACAGTTGG CTCGCATCAC CGTTGAAGAG CAATACTCGG ACCTGGAGAA AGAGAAGATC 
ATGAAGGAAC TGGAGATAAA GGACATGATC GCCAGACACC GGCAGGATCT TGCAGAAAAG 
GACGGTACCA TCAACTCGCT GGAGGAGTCA AACCGTACGC TGACTGTGGA TGTTGCCAAC 
CTGGCCAGTG AGAAAGAGGA GCTCAACAAT AAACTGAAGC ACATACAGCA AAAGCTGGAG 
AAGATCAAAG AAGAAGAGAA ACAGATGAAA TCTCTCACAG TGTCGTATGA GAAGCAGATA 
CAGGTGGAGA AAACACTCAA GATTCAGGCT ATAAACAAGC TGGCGGAGGT CATGAAGAAG 
ACGGACGGCA GGCCACACCA AATCAACAAC ACAGACATCC GCAGGAAGGA GAAGGAGATC 
AGACAACTCC AGCTGCAGCT CCGCACGGAG AAAGAAAAGC TTAACCACTC CATCATCAAA 
TACCAGAGAG AAATCAACGA CATGCAGGCT ATGATAGCAG AGGAGTCTCA GGTGCGTCTG 
GAGATGCAGA TGTCTCTGGA CAGTAAAGAC AGTGATATTG AGCGTCTGCG CAGTCAACTC 
ACGTCTCTGA GCATTCACTC TCTGGACACC ACCAGCATCA GCAGCATCGG CAATGACCTG 
GATTCTGACG AGGCATATCC AGTGCGAATC ACACACTCAC ACACCTCGGA GTCTATGTCC 
TTCACATACC AGCGAAGCTC CAAGTCGGTG TGTATTGATA CCCGGCCCTG TCGAATCGCT 
CCTCGCTTCT CCTCTGACTC TGATGAAGAG GAGGATGAGG AAGATGAGTG GAGACAAACA 
CCTGCCCTTA CATATGAGAC GCCTCCTGAC CCTGAGGCTG CAGCCGCAGA GTCCGGTCTT 
GAAGGATGGC TCTCAATGCC TGCCAAAAAC ACCAAACGGT TTGGATGGGA TAAGAAGTAT 
GTGGTTGTGA GCAGCAAGAA AATCCTGTTT TATGACTCTG AACAAGACCG AGAGCAGTCC 
AGTCCATTCA TGATCCTGGA CATTGACAAA CTCTTCCACG TGAGACCCGT CACTCAGACA 
GATGTTTACC GCGCAGATCC CAGAGAAATC CCTCGCATCT TCCAGATACT ATATGCGAAT 
GAAGGAGAGA GCAAGAAAGA GGAGCTGCCC GTGGAGTCGT TATCTGTGAC TGAGAGGTCA 
CTGTGTATCC CACACAAGGG TCACGAGTTT GTTCTGACGC TTTATCACTT CCCCTCGAGC 
TGCGAGGTCT GTCCACGACC CTTATGGCAC GTCTTCAGAC CTCCGCCGGC TCTCGAGTGC 
AGACGCTGTA GAGTCAAGTG CCATAAAGAC CACATCGATC GCAAAGAAGA GGTCCTCGCT 
CCATGTAGAG TGAATTACGA CATGTCCACA GCGAAAGAGA TGCTGCTGCT GGCAAACTCC 
ACAGAGGAGC AGAAGAAGTG GGTCAGTCGG CTGCTCAAGA GGGTTCCGCG TAAACCTTCC 
ATCGCCCACT CTTCCAGCAT AGCCATATCC GCTGCGCCCT CAGAGGCAAC ACCCACTACC 
CTTTCCCCTC AGCCGTCGCC TCGCCTCGCT CAGTCTTCAC CCAGACTTTC ACACCGAGCA 
GCCGTCAAAG TGCACTCTAC ACGACAGCAG CCCTCCAGCA AGAACAGATT GCTTGAAATG 
GGCCTGAAGG ACTGGGGTTG GGCTCTTGAG GTTGAATTGG ATGATGACGA TGTTTTAGAC 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rock2b ( XM_009294697.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292972.1 Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X11, mRNA.

Price & Availability↑

CloneID	ODa39642
Clone ID Related Accession (Same CDS sequence)	XM_009294697.2 , XM_009294697.3
Accession Version	XM_009294697.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2826bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho-associated, coiled-coil containing protein kinase 2b isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294697.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATCAGTG GACCTTCGAC ACCATACGAG ACACTGGAGG AGTCGGAGAG GCTGAAGAAG 
GCCCAGATCG AGGCCTTTAG ACAGTCCCAG CAGCTGGAGC TTTCCCTCAG GGAGCTGCAG 
GAGCGTCTGG CCCAGCTGGA GAACAGCAGG CTGGTTCTGG AGCAGGACAA ACTCAGCCTG 
CAGACCTCGC TAGAGCTGGA GAAACGAGAG AGAAACGCCG GCTCAGAGAC CATCACTGAC 
CTGCAGGGTC GTATCTATGG ACTGGAGGGT GAGTTGAAGC ATATAAAGAG TTCTCTATCA 
AAAGCTGAAG TTGAGAAAAG ACAATTACAA GAGGATCTTA CAACCCTGGA AAAGGAGAAG 
AATAAGCAGG AGATCGACCT CTCCTTCAAA CTCAAAGCAG TTCAGCAGAG TCTGGAGCAG 
GAGGAGGCTG AACATAAAAC CACTAAAGCC AGATTGGCCG ACAACAACAA AATCAACCAA 
TCCATAGAGG CAAAGTCTGA GACCCTGAAA GACATGGAAC ATAAGCTGTT GGAAGAACGA 
TCTGCGAAGC AACAACTGGA AAACAGACTG ATGCAGCTGG AGAAGGAAAA CTCTGTATTA 
GACTGTGATT ACAAACAAGC CAAACATGAG CTTCAGGAGC TCCGCTCGCT CAAAGAAAAT 
CTCACAGAGC AGGTGGAGGT GCTGAATGTG CGGGTGCAGC AGGAGACACA GAGGAAAACT 
CTCTGTCAAG GTGATCTCAA AGTTCAGAGG CAGGAGATCA ACAGCCTGCG GTCATCAGAA 
CAACAGCTCA AACAAGAACT CAACCATCTG CTGGAGCTCA AACTCACACT GGAGAAACAA 
AACCAGGAGC TGTCCAAGGA ACGAGAAGAA TCTGAAAAAC AGCTGAAGGA GATGAAGGAC 
CAGCTGGAGG CTGAGCAGTA CTTCACGAAA CTGTACAAGA CTCAGATTCG AGAGCTGAAA 
GAGGAAAGCG ATGAAAAGGT TAAACTCTAC AAAGATGCTC AGCAGAGAAT AGAGGACCTG 
CAGGAAGAGA GGGACTCGTT AGCGTCTCAG TTGGAGGTGA GCCTGACGAA GGCTGACTCT 
GAACAGTTGG CTCGCATCAC CGTTGAAGAG CAATACTCGG ACCTGGAGAA AGAGAAGATC 
ATGAAGGAAC TGGAGATAAA GGACATGATC GCCAGACACC GGCAGGATCT TGCAGAAAAG 
GACGGTACCA TCAACTCGCT GGAGGAGTCA AACCGTACGC TGACTGTGGA TGTTGCCAAC 
CTGGCCAGTG AGAAAGAGGA GCTCAACAAT AAACTGAAGC ACATACAGCA AAAGCTGGAG 
AAGATCAAAG AAGAAGAGAA ACAGATGAAA TCTCTCACAG TGTCGTATGA GAAGCAGATA 
CAGGTGGAGA AAACACTCAA GATTCAGGCT ATAAACAAGC TGGCGGAGGT CATGAAGAAG 
ACGGACGGCA GGCCACACCA AATCAACAAC ACAGACATCC GCAGGAAGGA GAAGGAGATC 
AGACAACTCC AGCTGCAGCT CCGCACGGAG AAAGAAAAGC TTAACCACTC CATCATCAAA 
TACCAGAGAG AAATCAACGA CATGCAGGCT ATGATAGCAG AGGAGTCTCA GGTGCGTCTG 
GAGATGCAGA TGTCTCTGGA CAGTAAAGAC AGTGATATTG AGCGTCTGCG CAGTCAACTC 
ACGTCTCTGA GCATTCACTC TCTGGACACC ACCAGCATCA GCAGCATCGG CAATGACCTG 
GATTCTGACG AGGCATATCC AGTGCGAATC ACACACTCAC ACACCTCGGA GTCTATGTCC 
TTCACATACC AGCGAAGCTC CAAGTCGGTG TGTATTGATA CCCGGCCCTG TCGAATCGCT 
CCTCGCTTCT CCTCTGACTC TGATGAAGAG GAGGATGAGG AAGATGAGTG GAGACAAACA 
CCTGCCCTTA CATATGAGAC GCCTCCTGAC CCTGAGGCTG CAGCCGCAGA GTCCGGTCTT 
GAAGGATGGC TCTCAATGCC TGCCAAAAAC ACCAAACGGT TTGGATGGGA TAAGAAGTAT 
GTGGTTGTGA GCAGCAAGAA AATCCTGTTT TATGACTCTG AACAAGACCG AGAGCAGTCC 
AGTCCATTCA TGATCCTGGA CATTGACAAA CTCTTCCACG TGAGACCCGT CACTCAGACA 
GATGTTTACC GCGCAGATCC CAGAGAAATC CCTCGCATCT TCCAGATACT ATATGCGAAT 
GAAGGAGAGA GCAAGAAAGA GGAGCTGCCC GTGGAGTCGT TATCTGTGAC TGAGAGGTCA 
CTGTGTATCC CACACAAGGG TCACGAGTTT GTTCTGACGC TTTATCACTT CCCCTCGAGC 
TGCGAGGTCT GTCCACGACC CTTATGGCAC GTCTTCAGAC CTCCGCCGGC TCTCGAGTGC 
AGACGCTGTA GAGTCAAGTG CCATAAAGAC CACATCGATC GCAAAGAAGA GGTCCTCGCT 
CCATGTAGAG TGAATTACGA CATGTCCACA GCGAAAGAGA TGCTGCTGCT GGCAAACTCC 
ACAGAGGAGC AGAAGAAGTG GGTCAGTCGG CTGCTCAAGA GGGTTCCGCG TAAACCTTCC 
ATCGCCCACT CTTCCAGCAT AGCCATATCC GCTGCGCCCT CAGAGGCAAC ACCCACTACC 
CTTTCCCCTC AGCCGTCGCC TCGCCTCGCT CAGTCTTCAC CCAGACTTTC ACACCGAGCA 
GCCGTCAAAG TGCACTCTAC ACGACAGCAG CCCTCCAGCA AGAACAGATT GCTTGAAATG 
GGCCTGAAGG ACTGGGGTTG GGCTCTTGAG GTTGAATTGG ATGATGACGA TGTTTTAGAC 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292972.1
CDS	195..3020
Translation	MISGPSTPYETLEESERLKKAQIEAFRQSQQLELSLRELQERLAQLENSRLVLEQDKLSLQTSLELEKRERNAGSETITDLQGRIYGLEGELKHIKSSLSKAEVEKRQLQEDLTTLEKEKNKQEIDLSFKLKAVQQSLEQEEAEHKTTKARLADNNKINQSIEAKSETLKDMEHKLLEERSAKQQLENRLMQLEKENSVLDCDYKQAKHELQELRSLKENLTEQVEVLNVRVQQETQRKTLCQGDLKVQRQEINSLRSSEQQLKQELNHLLELKLTLEKQNQELSKEREESEKQLKEMKDQLEAEQYFTKLYKTQIRELKEESDEKVKLYKDAQQRIEDLQEERDSLASQLEVSLTKADSEQLARITVEEQYSDLEKEKIMKELEIKDMIARHRQDLAEKDGTINSLEESNRTLTVDVANLASEKEELNNKLKHIQQKLEKIKEEEKQMKSLTVSYEKQIQVEKTLKIQAINKLAEVMKKTDGRPHQINNTDIRRKEKEIRQLQLQLRTEKEKLNHSIIKYQREINDMQAMIAEESQVRLEMQMSLDSKDSDIERLRSQLTSLSIHSLDTTSISSIGNDLDSDEAYPVRITHSHTSESMSFTYQRSSKSVCIDTRPCRIAPRFSSDSDEEEDEEDEWRQTPALTYETPPDPEAAAAESGLEGWLSMPAKNTKRFGWDKKYVVVSSKKILFYDSEQDREQSSPFMILDIDKLFHVRPVTQTDVYRADPREIPRIFQILYANEGESKKEELPVESLSVTERSLCIPHKGHEFVLTLYHFPSSCEVCPRPLWHVFRPPPALECRRCRVKCHKDHIDRKEEVLAPCRVNYDMSTAKEMLLLANSTEEQKKWVSRLLKRVPRKPSIAHSSSIAISAAPSEATPTTLSPQPSPRLAQSSPRLSHRAAVKVHSTRQQPSSKNRLLEMGLKDWGWALEVELDDDDVLDF

Target ORF information:

RefSeq Version	XM_009294697.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294697.3

ORF Insert Sequence:

ATGATCAGTG GACCTTCGAC ACCATACGAG ACACTGGAGG AGTCGGAGAG GCTGAAGAAG 
GCCCAGATCG AGGCCTTTAG ACAGTCCCAG CAGCTGGAGC TTTCCCTCAG GGAGCTGCAG 
GAGCGTCTGG CCCAGCTGGA GAACAGCAGG CTGGTTCTGG AGCAGGACAA ACTCAGCCTG 
CAGACCTCGC TAGAGCTGGA GAAACGAGAG AGAAACGCCG GCTCAGAGAC CATCACTGAC 
CTGCAGGGTC GTATCTATGG ACTGGAGGGT GAGTTGAAGC ATATAAAGAG TTCTCTATCA 
AAAGCTGAAG TTGAGAAAAG ACAATTACAA GAGGATCTTA CAACCCTGGA AAAGGAGAAG 
AATAAGCAGG AGATCGACCT CTCCTTCAAA CTCAAAGCAG TTCAGCAGAG TCTGGAGCAG 
GAGGAGGCTG AACATAAAAC CACTAAAGCC AGATTGGCCG ACAACAACAA AATCAACCAA 
TCCATAGAGG CAAAGTCTGA GACCCTGAAA GACATGGAAC ATAAGCTGTT GGAAGAACGA 
TCTGCGAAGC AACAACTGGA AAACAGACTG ATGCAGCTGG AGAAGGAAAA CTCTGTATTA 
GACTGTGATT ACAAACAAGC CAAACATGAG CTTCAGGAGC TCCGCTCGCT CAAAGAAAAT 
CTCACAGAGC AGGTGGAGGT GCTGAATGTG CGGGTGCAGC AGGAGACACA GAGGAAAACT 
CTCTGTCAAG GTGATCTCAA AGTTCAGAGG CAGGAGATCA ACAGCCTGCG GTCATCAGAA 
CAACAGCTCA AACAAGAACT CAACCATCTG CTGGAGCTCA AACTCACACT GGAGAAACAA 
AACCAGGAGC TGTCCAAGGA ACGAGAAGAA TCTGAAAAAC AGCTGAAGGA GATGAAGGAC 
CAGCTGGAGG CTGAGCAGTA CTTCACGAAA CTGTACAAGA CTCAGATTCG AGAGCTGAAA 
GAGGAAAGCG ATGAAAAGGT TAAACTCTAC AAAGATGCTC AGCAGAGAAT AGAGGACCTG 
CAGGAAGAGA GGGACTCGTT AGCGTCTCAG TTGGAGGTGA GCCTGACGAA GGCTGACTCT 
GAACAGTTGG CTCGCATCAC CGTTGAAGAG CAATACTCGG ACCTGGAGAA AGAGAAGATC 
ATGAAGGAAC TGGAGATAAA GGACATGATC GCCAGACACC GGCAGGATCT TGCAGAAAAG 
GACGGTACCA TCAACTCGCT GGAGGAGTCA AACCGTACGC TGACTGTGGA TGTTGCCAAC 
CTGGCCAGTG AGAAAGAGGA GCTCAACAAT AAACTGAAGC ACATACAGCA AAAGCTGGAG 
AAGATCAAAG AAGAAGAGAA ACAGATGAAA TCTCTCACAG TGTCGTATGA GAAGCAGATA 
CAGGTGGAGA AAACACTCAA GATTCAGGCT ATAAACAAGC TGGCGGAGGT CATGAAGAAG 
ACGGACGGCA GGCCACACCA AATCAACAAC ACAGACATCC GCAGGAAGGA GAAGGAGATC 
AGACAACTCC AGCTGCAGCT CCGCACGGAG AAAGAAAAGC TTAACCACTC CATCATCAAA 
TACCAGAGAG AAATCAACGA CATGCAGGCT ATGATAGCAG AGGAGTCTCA GGTGCGTCTG 
GAGATGCAGA TGTCTCTGGA CAGTAAAGAC AGTGATATTG AGCGTCTGCG CAGTCAACTC 
ACGTCTCTGA GCATTCACTC TCTGGACACC ACCAGCATCA GCAGCATCGG CAATGACCTG 
GATTCTGACG AGGCATATCC AGTGCGAATC ACACACTCAC ACACCTCGGA GTCTATGTCC 
TTCACATACC AGCGAAGCTC CAAGTCGGTG TGTATTGATA CCCGGCCCTG TCGAATCGCT 
CCTCGCTTCT CCTCTGACTC TGATGAAGAG GAGGATGAGG AAGATGAGTG GAGACAAACA 
CCTGCCCTTA CATATGAGAC GCCTCCTGAC CCTGAGGCTG CAGCCGCAGA GTCCGGTCTT 
GAAGGATGGC TCTCAATGCC TGCCAAAAAC ACCAAACGGT TTGGATGGGA TAAGAAGTAT 
GTGGTTGTGA GCAGCAAGAA AATCCTGTTT TATGACTCTG AACAAGACCG AGAGCAGTCC 
AGTCCATTCA TGATCCTGGA CATTGACAAA CTCTTCCACG TGAGACCCGT CACTCAGACA 
GATGTTTACC GCGCAGATCC CAGAGAAATC CCTCGCATCT TCCAGATACT ATATGCGAAT 
GAAGGAGAGA GCAAGAAAGA GGAGCTGCCC GTGGAGTCGT TATCTGTGAC TGAGAGGTCA 
CTGTGTATCC CACACAAGGG TCACGAGTTT GTTCTGACGC TTTATCACTT CCCCTCGAGC 
TGCGAGGTCT GTCCACGACC CTTATGGCAC GTCTTCAGAC CTCCGCCGGC TCTCGAGTGC 
AGACGCTGTA GAGTCAAGTG CCATAAAGAC CACATCGATC GCAAAGAAGA GGTCCTCGCT 
CCATGTAGAG TGAATTACGA CATGTCCACA GCGAAAGAGA TGCTGCTGCT GGCAAACTCC 
ACAGAGGAGC AGAAGAAGTG GGTCAGTCGG CTGCTCAAGA GGGTTCCGCG TAAACCTTCC 
ATCGCCCACT CTTCCAGCAT AGCCATATCC GCTGCGCCCT CAGAGGCAAC ACCCACTACC 
CTTTCCCCTC AGCCGTCGCC TCGCCTCGCT CAGTCTTCAC CCAGACTTTC ACACCGAGCA 
GCCGTCAAAG TGCACTCTAC ACGACAGCAG CCCTCCAGCA AGAACAGATT GCTTGAAATG 
GGCCTGAAGG ACTGGGGTTG GGCTCTTGAG GTTGAATTGG ATGATGACGA TGTTTTAGAC 
TTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rock2b ( XM_017352436.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207925.1 Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa24919
Clone ID Related Accession (Same CDS sequence)	XM_017352436.1
Accession Version	XM_017352436.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3471bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	rho-associated, coiled-coil containing protein kinase 2b isoform X6
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGACCG GAGCGGGGAA GAGAATAGAG AGCCGGGTGA GAAAACTAGA GACGCTGATC 
AAGAACCAGA AATCACCGTT GAACTTGGAG AGTTTGCTGG ATTCAGTAAC TGCCCTTGCC 
CTTGATCTGA ACCATCCAGC ACTGAGAAAG AACAAAAACA TAGATGCCTT CTTAAGCAGA 
TATGAAAAAG CCGTGTGCAA CCTCAAGGAA CTACAGGTGA AGCTGGATGA TTTTGATCGA 
GTCAAGCTGA TCGGTCGTGG AGCGTTTGGC GCAGTGCAGC TGGTACGGCA CAAAGTATCT 
CAACAAGTTT ATGCCATGAA GCAGCTCAGC AAGTTTGAGA TGGTCAAGCG CTCTGACTCC 
GCCTTTTTCT GGGAGGAGCG TGACATCATG GCTTTCTCCC AGAGCCCTTG GATAGTTCAG 
CTGTGCTGTG CATTCCAAGA TGAAAAGTAC CTTTATTTGG TGATGGAGTT CATGCCAGGT 
GGAGATCTGG TGACTCTGAC CAGCAACTAT GACATCCCAG AGGAATGGGC TCAATTCTAT 
ACGGCAGAAG TGGTGCTGGC ACTCGACGCC ATCCATTCTC TGGGCTTCAT CCACAGAGAC 
ATCAAACCAG ACAACATGCT GCTGGACCGC AATGGACACC TCAAACTCGC AGACTTTGGC 
ACATGCATGA AGATGGACTC GACTGGTATG GTACGCTGTG ACACTGCTGT TGGGACACCT 
GATTACATTT CTCCAGAGGT TTTAATGTCT CAAGGAGGGA CTGGTTATTA CGGTCGCGAG 
TGTGACTGGT GGTCTGTTGG TGTTTTTATT TATGAGCTTC TAGTGGGCGA CACTCCATTT 
TATTCCGAGT CTCTGGTGGG CACATATGGA AAGATCATGG ATCACAAGAA CAGCTTAACT 
TTCCCAGATG ATATTGAAAT GTCAAAGAAT GCCAAAGACC TCATCTGTGC CTTTCTGAGT 
AGCAGGGAGG TGAGGCTTGG CCGAACTGGA GTGGATGAAA TAAAATGTCA CCCCTTCTTT 
AAGAATGATC AGTGGACCTT CGACACCATA CGAGACACCA TGGCTCCAGT GGTGCCAGAG 
CTGAGTAGCG ATATTGACAC CAGTAACTTT GATGATGACA TTAAGGATGA TCCGATAGGA 
ACAGAGACGT TCCCTCCGCC CCGTGCCTTC GCAGGGAACC AGCTACCATT TGTGGGTTTC 
ACCTATTTCA GGGAGGACCA ATTGTTAACA CAACAAAATA AGTGTTCAGA GGAAGATATC 
ATTGAAGATC ATGGCTTAAA CCACACAGAG TCTAACGACC TGGAGAAGAG GCTGAAAAAG 
TTGGAGGAAA AATTCAAACA TGAGATGCAA GCAAAGGAAG AACTGGAAAA TAAATGCCGA 
ATAGCAAATC AGCGGCTGGA GAAATTGTCC AAAGATCTTG AAGAAGAAGT GAATGCGAGA 
CAGGAGGCGG AAGACAACCT GAGATCCCTG GAGAAAGAGA AAGTTTTACT GAAACACCAG 
CGTACACAGA GCGTAAGAAA AGCTGGTCTA GAGACCGACA GAAAACGGCT GCTGGAGAAT 
GAAGTAAGCA GCCTGAAAGA GCAGCTTGCC GAACTGAAGA AAAAGAACCA GATCTCTCAT 
CTGTCTGCTG AGAAGAACAT TCACCTGGAA AGACAGCTGG AGGAGGTGAG TGCCAAGCTT 
CAGGCCGAGC TGGAGGAGTC GGAGAGGCTG AAGAAGGCCC AGATCGAGGC CTTTAGACAG 
TCCCAGCAGC TGGAGCTTTC CCTCAGGGAG CTGCAGGAGC GTCTGGCCCA GCTGGAGAAC 
AGCAGGCTGG TTCTGGAGCA GGACAAACTC AGCCTGCAGA CCTCGCTAGA GCTGGAGAAA 
CGAGAGAGAA ACGCCGGCTC AGAGACCATC ACTGACCTGC AGGGTCGTAT CTATGGACTG 
GAGGGTGAGT TGAAGCATAT AAAGAGTTCT CTATCAAAAG CTGAAGTTGA GAAAAGACAA 
TTACAAGAGG ATCTTACAAC CCTGGAAAAG GAGAAGAATA AGCAGGAGAT CGACCTCTCC 
TTCAAACTCA AAGCAGTTCA GCAGAGTCTG GAGCAGGAGG AGGCTGAACA TAAAACCACT 
AAAGCCAGAT TGGCCGACAA CAACAAAATC AACCAATCCA TAGAGGCAAA GTCTGAGACC 
CTGAAAGACA TGGAACATAA GCTGTTGGAA GAACGATCTG CGAAGCAACA ACTGGAAAAC 
AGACTGATGC AGCTGGAGAA GGAAAACTCT GTATTAGACT GTGATTACAA ACAAGCCAAA 
CATGAGCTTC AGGAGCTCCG CTCGCTCAAA GAAAATCTCA CAGAGCAGGT GGAGGTGCTG 
AATGTGCGGG TGCAGCAGGA GACACAGAGG AAAACTCTCT GTCAAGGTGA TCTCAAAGTT 
CAGAGGCAGG AGATCAACAG CCTGCGGTCA TCAGAACAAC AGCTCAAACA AGAACTCAAC 
CATCTGCTGG AGCTCAAACT CACACTGGAG AAACAAAACC AGGAGCTGTC CAAGGAACGA 
GAAGAATCTG AAAAACAGCT GAAGGAGATG AAGGACCAGC TGGAGGCTGA GCAGTACTTC 
ACGAAACTGT ACAAGACTCA GATTCGAGAG CTGAAAGAGG AAAGCGATGA AAAGGTTAAA 
CTCTACAAAG ATGCTCAGCA GAGAATAGAG GACCTGCAGG AAGAGAGGGA CTCGTTAGCG 
TCTCAGTTGG AGGTGAGCCT GACGAAGGCT GACTCTGAAC AGTTGGCTCG CATCACCGTT 
GAAGAGCAAT ACTCGGACCT GGAGAAAGAG AAGATCATGA AGGAACTGGA GATAAAGGAC 
ATGATCGCCA GACACCGGCA GGATCTTGCA GAAAAGGACG GTACCATCAA CTCGCTGGAG 
GAGTCAAACC GTACGCTGAC TGTGGATGTT GCCAACCTGG CCAGTGAGAA AGAGGAGCTC 
AACAATAAAC TGAAGCACAT ACAGCAAAAG CTGGAGAAGA TCAAAGAAGA AGAGAAACAG 
ATGAAATCTC TCACAGTGTC GTATGAGAAG CAGATACAGG TGGAGAAAAC ACTCAAGATT 
CAGGCTATAA ACAAGCTGGC GGAGGTCATG AAGAAGACGG ACGGCAGGCC ACACCAAATC 
AACAACACAG ACATCCGCAG GAAGGAGAAG GAGATCAGAC AACTCCAGCT GCAGCTCCGC 
ACGGAGAAAG AAAAGCTTAA CCACTCCATC ATCAAATACC AGAGAGAAAT CAACGACATG 
CAGGCTATGA TAGCAGAGGA GTCTCAGGTG CGTCTGGAGA TGCAGATGTC TCTGGACAGT 
AAAGACAGTG ATATTGAGCG TCTGCGCAGT CAACTCACGT CTCTGAGCAT TCACTCTCTG 
GACACCACCA GCATCAGCAG CATCGGCAAT GACCTGGATT CTGACGAGGC ATATCCAGCT 
GGAGGAGTCA AACCGTACGC TGACTGTGGA TGTTGCCAAC CTGGCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207925.1
CDS	588..4058
Translation	MSTGAGKRIESRVRKLETLIKNQKSPLNLESLLDSVTALALDLNHPALRKNKNIDAFLSRYEKAVCNLKELQVKLDDFDRVKLIGRGAFGAVQLVRHKVSQQVYAMKQLSKFEMVKRSDSAFFWEERDIMAFSQSPWIVQLCCAFQDEKYLYLVMEFMPGGDLVTLTSNYDIPEEWAQFYTAEVVLALDAIHSLGFIHRDIKPDNMLLDRNGHLKLADFGTCMKMDSTGMVRCDTAVGTPDYISPEVLMSQGGTGYYGRECDWWSVGVFIYELLVGDTPFYSESLVGTYGKIMDHKNSLTFPDDIEMSKNAKDLICAFLSSREVRLGRTGVDEIKCHPFFKNDQWTFDTIRDTMAPVVPELSSDIDTSNFDDDIKDDPIGTETFPPPRAFAGNQLPFVGFTYFREDQLLTQQNKCSEEDIIEDHGLNHTESNDLEKRLKKLEEKFKHEMQAKEELENKCRIANQRLEKLSKDLEEEVNARQEAEDNLRSLEKEKVLLKHQRTQSVRKAGLETDRKRLLENEVSSLKEQLAELKKKNQISHLSAEKNIHLERQLEEVSAKLQAELEESERLKKAQIEAFRQSQQLELSLRELQERLAQLENSRLVLEQDKLSLQTSLELEKRERNAGSETITDLQGRIYGLEGELKHIKSSLSKAEVEKRQLQEDLTTLEKEKNKQEIDLSFKLKAVQQSLEQEEAEHKTTKARLADNNKINQSIEAKSETLKDMEHKLLEERSAKQQLENRLMQLEKENSVLDCDYKQAKHELQELRSLKENLTEQVEVLNVRVQQETQRKTLCQGDLKVQRQEINSLRSSEQQLKQELNHLLELKLTLEKQNQELSKEREESEKQLKEMKDQLEAEQYFTKLYKTQIRELKEESDEKVKLYKDAQQRIEDLQEERDSLASQLEVSLTKADSEQLARITVEEQYSDLEKEKIMKELEIKDMIARHRQDLAEKDGTINSLEESNRTLTVDVANLASEKEELNNKLKHIQQKLEKIKEEEKQMKSLTVSYEKQIQVEKTLKIQAINKLAEVMKKTDGRPHQINNTDIRRKEKEIRQLQLQLRTEKEKLNHSIIKYQREINDMQAMIAEESQVRLEMQMSLDSKDSDIERLRSQLTSLSIHSLDTTSISSIGNDLDSDEAYPAGGVKPYADCGCCQPGQ

Target ORF information:

RefSeq Version	XM_017352436.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352436.1

ORF Insert Sequence:

ATGTCGACCG GAGCGGGGAA GAGAATAGAG AGCCGGGTGA GAAAACTAGA GACGCTGATC 
AAGAACCAGA AATCACCGTT GAACTTGGAG AGTTTGCTGG ATTCAGTAAC TGCCCTTGCC 
CTTGATCTGA ACCATCCAGC ACTGAGAAAG AACAAAAACA TAGATGCCTT CTTAAGCAGA 
TATGAAAAAG CCGTGTGCAA CCTCAAGGAA CTACAGGTGA AGCTGGATGA TTTTGATCGA 
GTCAAGCTGA TCGGTCGTGG AGCGTTTGGC GCAGTGCAGC TGGTACGGCA CAAAGTATCT 
CAACAAGTTT ATGCCATGAA GCAGCTCAGC AAGTTTGAGA TGGTCAAGCG CTCTGACTCC 
GCCTTTTTCT GGGAGGAGCG TGACATCATG GCTTTCTCCC AGAGCCCTTG GATAGTTCAG 
CTGTGCTGTG CATTCCAAGA TGAAAAGTAC CTTTATTTGG TGATGGAGTT CATGCCAGGT 
GGAGATCTGG TGACTCTGAC CAGCAACTAT GACATCCCAG AGGAATGGGC TCAATTCTAT 
ACGGCAGAAG TGGTGCTGGC ACTCGACGCC ATCCATTCTC TGGGCTTCAT CCACAGAGAC 
ATCAAACCAG ACAACATGCT GCTGGACCGC AATGGACACC TCAAACTCGC AGACTTTGGC 
ACATGCATGA AGATGGACTC GACTGGTATG GTACGCTGTG ACACTGCTGT TGGGACACCT 
GATTACATTT CTCCAGAGGT TTTAATGTCT CAAGGAGGGA CTGGTTATTA CGGTCGCGAG 
TGTGACTGGT GGTCTGTTGG TGTTTTTATT TATGAGCTTC TAGTGGGCGA CACTCCATTT 
TATTCCGAGT CTCTGGTGGG CACATATGGA AAGATCATGG ATCACAAGAA CAGCTTAACT 
TTCCCAGATG ATATTGAAAT GTCAAAGAAT GCCAAAGACC TCATCTGTGC CTTTCTGAGT 
AGCAGGGAGG TGAGGCTTGG CCGAACTGGA GTGGATGAAA TAAAATGTCA CCCCTTCTTT 
AAGAATGATC AGTGGACCTT CGACACCATA CGAGACACCA TGGCTCCAGT GGTGCCAGAG 
CTGAGTAGCG ATATTGACAC CAGTAACTTT GATGATGACA TTAAGGATGA TCCGATAGGA 
ACAGAGACGT TCCCTCCGCC CCGTGCCTTC GCAGGGAACC AGCTACCATT TGTGGGTTTC 
ACCTATTTCA GGGAGGACCA ATTGTTAACA CAACAAAATA AGTGTTCAGA GGAAGATATC 
ATTGAAGATC ATGGCTTAAA CCACACAGAG TCTAACGACC TGGAGAAGAG GCTGAAAAAG 
TTGGAGGAAA AATTCAAACA TGAGATGCAA GCAAAGGAAG AACTGGAAAA TAAATGCCGA 
ATAGCAAATC AGCGGCTGGA GAAATTGTCC AAAGATCTTG AAGAAGAAGT GAATGCGAGA 
CAGGAGGCGG AAGACAACCT GAGATCCCTG GAGAAAGAGA AAGTTTTACT GAAACACCAG 
CGTACACAGA GCGTAAGAAA AGCTGGTCTA GAGACCGACA GAAAACGGCT GCTGGAGAAT 
GAAGTAAGCA GCCTGAAAGA GCAGCTTGCC GAACTGAAGA AAAAGAACCA GATCTCTCAT 
CTGTCTGCTG AGAAGAACAT TCACCTGGAA AGACAGCTGG AGGAGGTGAG TGCCAAGCTT 
CAGGCCGAGC TGGAGGAGTC GGAGAGGCTG AAGAAGGCCC AGATCGAGGC CTTTAGACAG 
TCCCAGCAGC TGGAGCTTTC CCTCAGGGAG CTGCAGGAGC GTCTGGCCCA GCTGGAGAAC 
AGCAGGCTGG TTCTGGAGCA GGACAAACTC AGCCTGCAGA CCTCGCTAGA GCTGGAGAAA 
CGAGAGAGAA ACGCCGGCTC AGAGACCATC ACTGACCTGC AGGGTCGTAT CTATGGACTG 
GAGGGTGAGT TGAAGCATAT AAAGAGTTCT CTATCAAAAG CTGAAGTTGA GAAAAGACAA 
TTACAAGAGG ATCTTACAAC CCTGGAAAAG GAGAAGAATA AGCAGGAGAT CGACCTCTCC 
TTCAAACTCA AAGCAGTTCA GCAGAGTCTG GAGCAGGAGG AGGCTGAACA TAAAACCACT 
AAAGCCAGAT TGGCCGACAA CAACAAAATC AACCAATCCA TAGAGGCAAA GTCTGAGACC 
CTGAAAGACA TGGAACATAA GCTGTTGGAA GAACGATCTG CGAAGCAACA ACTGGAAAAC 
AGACTGATGC AGCTGGAGAA GGAAAACTCT GTATTAGACT GTGATTACAA ACAAGCCAAA 
CATGAGCTTC AGGAGCTCCG CTCGCTCAAA GAAAATCTCA CAGAGCAGGT GGAGGTGCTG 
AATGTGCGGG TGCAGCAGGA GACACAGAGG AAAACTCTCT GTCAAGGTGA TCTCAAAGTT 
CAGAGGCAGG AGATCAACAG CCTGCGGTCA TCAGAACAAC AGCTCAAACA AGAACTCAAC 
CATCTGCTGG AGCTCAAACT CACACTGGAG AAACAAAACC AGGAGCTGTC CAAGGAACGA 
GAAGAATCTG AAAAACAGCT GAAGGAGATG AAGGACCAGC TGGAGGCTGA GCAGTACTTC 
ACGAAACTGT ACAAGACTCA GATTCGAGAG CTGAAAGAGG AAAGCGATGA AAAGGTTAAA 
CTCTACAAAG ATGCTCAGCA GAGAATAGAG GACCTGCAGG AAGAGAGGGA CTCGTTAGCG 
TCTCAGTTGG AGGTGAGCCT GACGAAGGCT GACTCTGAAC AGTTGGCTCG CATCACCGTT 
GAAGAGCAAT ACTCGGACCT GGAGAAAGAG AAGATCATGA AGGAACTGGA GATAAAGGAC 
ATGATCGCCA GACACCGGCA GGATCTTGCA GAAAAGGACG GTACCATCAA CTCGCTGGAG 
GAGTCAAACC GTACGCTGAC TGTGGATGTT GCCAACCTGG CCAGTGAGAA AGAGGAGCTC 
AACAATAAAC TGAAGCACAT ACAGCAAAAG CTGGAGAAGA TCAAAGAAGA AGAGAAACAG 
ATGAAATCTC TCACAGTGTC GTATGAGAAG CAGATACAGG TGGAGAAAAC ACTCAAGATT 
CAGGCTATAA ACAAGCTGGC GGAGGTCATG AAGAAGACGG ACGGCAGGCC ACACCAAATC 
AACAACACAG ACATCCGCAG GAAGGAGAAG GAGATCAGAC AACTCCAGCT GCAGCTCCGC 
ACGGAGAAAG AAAAGCTTAA CCACTCCATC ATCAAATACC AGAGAGAAAT CAACGACATG 
CAGGCTATGA TAGCAGAGGA GTCTCAGGTG CGTCTGGAGA TGCAGATGTC TCTGGACAGT 
AAAGACAGTG ATATTGAGCG TCTGCGCAGT CAACTCACGT CTCTGAGCAT TCACTCTCTG 
GACACCACCA GCATCAGCAG CATCGGCAAT GACCTGGATT CTGACGAGGC ATATCCAGCT 
GGAGGAGTCA AACCGTACGC TGACTGTGGA TGTTGCCAAC CTGGCCAGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rock2b ( XM_021468357.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324032.1 Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa150578
Clone ID Related Accession (Same CDS sequence)	XM_021468357.1 , XM_021468358.1
Accession Version	XM_021468357.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho-associated, coiled-coil containing protein kinase 2b isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGACCG GAGCGGGGAA GAGAATAGAG AGCCGGGTGA GAAAACTAGA GACGCTGATC 
AAGAACCAGA AATCACCGTT GAACTTGGAG AGTTTGCTGG ATTCAGTAAC TGCCCTTGCC 
CTTGATCTGA ACCATCCAGC ACTGAGAAAG AACAAAAACA TAGATGCCTT CTTAAGCAGA 
TATGAAAAAG CCGTGTGCAA CCTCAAGGAA CTACAGGTGA AGCTGGATGA TTTTGATCGA 
GTCAAGCTGA TCGGTCGTGG AGCGTTTGGC GCAGTGCAGC TGGTACGGCA CAAAGTATCT 
CAACAAGTTT ATGCCATGAA GCAGCTCAGC AAGTTTGAGA TGGTCAAGCG CTCTGACTCC 
GCCTTTTTCT GGGAGGAGCG TGACATCATG GCTTTCTCCC AGAGCCCTTG GATAGTTCAG 
CTGTGCTGTG CATTCCAAGA TGAAAAGTAC CTTTATTTGG TGATGGAGTT CATGCCAGGT 
GGAGATCTGG TGACTCTGAC CAGCAACTAT GACATCCCAG AGGAATGGGC TCAATTCTAT 
ACGGCAGAAG TGGTGCTGGC ACTCGACGCC ATCCATTCTC TGGGCTTCAT CCACAGAGAC 
ATCAAACCAG ACAACATGCT GCTGGACCGC AATGGACACC TCAAACTCGC AGACTTTGGC 
ACATGCATGA AGATGGACTC GACTGGTATG GTACGCTGTG ACACTGCTGT TGGGACACCT 
GATTACATTT CTCCAGAGGT TTTAATGTCT CAAGGAGGGA CTGGTTATTA CGGTCGCGAG 
TGTGACTGGT GGTCTGTTGG TGTTTTTATT TATGAGCTTC TAGTGGGCGA CACTCCATTT 
TATTCCGAGT CTCTGGTGGG CACATATGGA AAGATCATGG ATCACAAGAA CAGCTTAACT 
TTCCCAGATG ATATTGAAAT GTCAAAGAAT GCCAAAGACC TCATCTGTGC CTTTCTGAGT 
AGCAGGGAGG TGAGGCTTGG CCGAACTGGA GTGGATGAAA TAAAATGTCA CCCCTTCTTT 
AAGAATGATC AGTGGACCTT CGACACCATA CGAGACACCA TGGCTCCAGT GGTGCCAGAG 
CTGAGTAGCG ATATTGACAC CAGTAACTTT GATGATGACA TTAAGGATGA TCCGATAGGA 
ACAGAGACGT TCCCTCCGCC CCGTGCCTTC GCAGGGAACC AGCTACCATT TGTGGGTTTC 
ACCTATTTCA GGGAGGACCA ATTGTTAACA CAACAAAATA AGTGTTCAGA GGAAGATATC 
ATTGAAGATC ATGGCTTAAA CCACACAGAG TCTAACGACC TGGAGAAGAG GCTGAAAAAG 
TTGGAGGAAA AATTCAAACA TGAGATGCAA GCAAAGGAAG AACTGGAAAA TAAATGCCGA 
ATAGCAAATC AGCGGCTGGA GAAATTGTCC AAAGATCTTG AAGAAGAAGT GAATGCGAGA 
CAGGAGGCGG AAGACAACCT GAGATCCCTG GAGAAAGAGA AAGTTTTACT GAAACACCAG 
CGTACACAGA GCGTAAGAAA AGCTGGTCTA GAGACCGACA GAAAACGGCT GCTGGAGAAT 
GAAGTAAGCA GCCTGAAAGA GCAGCTTGCC GAACTGAAGA AAAAGAACCA GATCTCTCAT 
CTGTCTGCTG AGAAGAACAT TCACCTGGAA AGACAGCTGG AGGAGGTGAG TGCCAAGCTT 
CAGGCCGAGC TGGAGGAGTC GGAGAGGCTG AAGAAGGCCC AGATCGAGGC CTTTAGACAG 
TCCCAGCAGC TGGAGCTTTC CCTCAGGGAG CTGCAGGAGC GTCTGGCCCA GCTGGAGAAC 
AGCAGGCTGG TTCTGGAGCA GGACAAACTC AGCCTGCAGA CCTCGCTAGA GCTGGAGAAA 
CGAGAGAGAA ACGCCGGCTC AGAGACCATC ACTGACCTGC AGGGTCGTAT CTATGGACTG 
GAGGGTGAGT TGAAGCATAT AAAGAGTTCT CTATCAAAAG CTGAAGTTGA GAAAAGACAA 
TTACAAGAGG ATCTTACAAC CCTGGAAAAG GAGAAGAATA AGCAGGAGAT CGACCTCTCC 
TTCAAACTCA AAGCAGTTCA GCAGAGTCTG GAGCAGGAGG AGGCTGAACA TAAAACCACT 
AAAGCCAGAT TGGCCGACAA CAACAAAATC AACCAATCCA TAGAGGCAAA GTCTGAGACC 
CTGAAAGACA TGGAACATAA GCTGTTGGAA GAACGATCTG CGAAGCAACA ACTGGAAAAC 
AGACTGATGC AGCTGGAGAA GGAAAACTCT GTATTAGACT GTGATTACAA ACAAGCCAAA 
CATGAGCTTC AGGAGCTCCG CTCGCTCAAA GAAAATCTCA CAGAGCAGGT GGAGGTGCTG 
AATGTGCGGG TGCAGCAGGA GACACAGAGG AAAACTCTCT GTCAAGGTGA TCTCAAAGTT 
CAGAGGCAGG AGATCAACAG CCTGCGGTCA TCAGAACAAC AGCTCAAACA AGAACTCAAC 
CATCTGCTGG AGCTCAAACT CACACTGGAG AAACAAAACC AGGAGCTGTC CAAGGAACGA 
GAAGAATCTG AAAAACAGCT GAAGGAGATG AAGGACCAGC TGGAGGCTGA GCAGTACTTC 
ACGAAACTGT ACAAGACTCA GATTCGAGAG CTGAAAGAGG AAAGCGATGA AAAGGTTAAA 
CTCTACAAAG ATGCTCAGCA GAGAATAGAG GACCTGCAGG AAGAGAGGGA CTCGTTAGCG 
TCTCAGTTGG AGGTGAGCCT GACGAAGGCT GACTCTGAAC AGTTGGCTCG CATCACCGTT 
GAAGAGCAAT ACTCGGACCT GGAGAAAGAG AAGATCATGA AGGAACTGGA GATAAAGGAC 
ATGATCGCCA GACACCGGCA GGATCTTGCA GAAAAGGACG GTACCATCAA CTCGCTGGAG 
GAGTCAAACC GTACGCTGAC TGTGGATGTT GCCAACCTGG CCAGTGAGAA AGAGGAGCTC 
AACAATAAAC TGAAGCACAT ACAGCAAAAG CTGGAGAAGA TCAAAGAAGA AGAGAAACAG 
ATGAAATCTC TCACAGTGTC GTATGAGAAG CAGATACAGG TGGAGAAAAC ACTCAAGATT 
CAGGCTATAA ACAAGCTGGC GGAGGTCATG AAGAAGACGG ACGGCAGGCC ACACCAAATC 
AACAACACAG ACATCCGCAG GAAGGAGAAG GAGATCAGAC AACTCCAGCT GCAGCTCCGC 
ACGGAGAAAG AAAAGCTTAA CCACTCCATC ATCAAATACC AGAGAGAAAT CAACGACATG 
CAGGCTATGA TAGCAGAGGA GTCTCAGGTG CGTCTGGAGA TGCAGATGTC TCTGGACAGT 
AAAGACAGTG ATATTGAGCG TCTGCGCAGT CAACTCACGT CTCTGAGCAT TCACTCTCTG 
GACACCACCA GCATCAGCAG CATCGGCAAT GACCTGGATT CTGACGAGGC ATATCCAGTG 
CGAATCACAC ACTCACACAC CTCGGAGTCT ATGTCCTTCA CATACCAGCG AAGCTCCAAG 
TCGGTGTGTA TTGATACCCG GCCCTGTCGA ATCGCTCCTC GCTTCTCCTC TGACTCTGAT 
GAAGAGGAGG ATGAGGAAGA TGAGTGGAGA CAAACACCTG CCCTTACATA TGAGACGCCT 
CCTGACCCTG AGGCTGCAGC CGCAGAGTCC GGTCTTGAAG GATGGCTCTC AATGCCTGCC 
AAAAACACCA AACGGTTTGG ATGGGATAAG AAGTATGTGG TTGTGAGCAG CAAGAAAATC 
CTGTTTTATG ACTCTGAACA AGACCGAGAG CAGTCCAGTC CATTCATGAT CCTGGACATT 
GACAAACTCT TCCACGTGAG ACCCGTCACT CAGACAGATG TTTACCGCGC AGATCCCAGA 
GAAATCCCTC GCATCTTCCA GATACTATAT GCGAATGAAG GAGAGAGCAA GAAAGAGGAG 
CTGCCCGTGG AGTCGTTATC TGTGACTGAG AGGTCACTGT GTATCCCACA CAAGGGTCAC 
GAGTTTGTTC TGACGCTTTA TCACTTCCCC TCGAGCTGCG AGGTCTGTCC ACGACCCTTA 
TGGCACGTCT TCAGACCTCC GCCGGCTCTC GAGTGCAGAC GCTGTAGAGT CAAGTGCCAT 
AAAGACCACA TCGATCGCAA AGAAGAGGTC CTCGCTCCAT GTAGAGTGAA TTACGACATG 
TCCACAGCGA AAGAGATGCT GCTGCTGGCA AACTCCACAG AGGAGCAGAA GAAGTGGGTC 
AGTCGGCTGC TCAAGAGGGT TCCGCGTAAA CCTTCCATCG CCCACTCTTC CAGCATAGCC 
ATATCCGCTG CGCCCTCAGA GGCAACACCC ACTACCCTTT CCCCTCAGCC GTCGCCTCGC 
CTCGCTCAGT CTTCACCCAG ACTTTCACAC CGAGCAGCCG TCAAAGTGCA CTCTACACGA 
CAGCAGCCCT CCAGCAAGAA CAGCCCTGGC CCAATGCTCC GTATCATTCT TCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324032.1
CDS	475..4911
Translation	MSTGAGKRIESRVRKLETLIKNQKSPLNLESLLDSVTALALDLNHPALRKNKNIDAFLSRYEKAVCNLKELQVKLDDFDRVKLIGRGAFGAVQLVRHKVSQQVYAMKQLSKFEMVKRSDSAFFWEERDIMAFSQSPWIVQLCCAFQDEKYLYLVMEFMPGGDLVTLTSNYDIPEEWAQFYTAEVVLALDAIHSLGFIHRDIKPDNMLLDRNGHLKLADFGTCMKMDSTGMVRCDTAVGTPDYISPEVLMSQGGTGYYGRECDWWSVGVFIYELLVGDTPFYSESLVGTYGKIMDHKNSLTFPDDIEMSKNAKDLICAFLSSREVRLGRTGVDEIKCHPFFKNDQWTFDTIRDTMAPVVPELSSDIDTSNFDDDIKDDPIGTETFPPPRAFAGNQLPFVGFTYFREDQLLTQQNKCSEEDIIEDHGLNHTESNDLEKRLKKLEEKFKHEMQAKEELENKCRIANQRLEKLSKDLEEEVNARQEAEDNLRSLEKEKVLLKHQRTQSVRKAGLETDRKRLLENEVSSLKEQLAELKKKNQISHLSAEKNIHLERQLEEVSAKLQAELEESERLKKAQIEAFRQSQQLELSLRELQERLAQLENSRLVLEQDKLSLQTSLELEKRERNAGSETITDLQGRIYGLEGELKHIKSSLSKAEVEKRQLQEDLTTLEKEKNKQEIDLSFKLKAVQQSLEQEEAEHKTTKARLADNNKINQSIEAKSETLKDMEHKLLEERSAKQQLENRLMQLEKENSVLDCDYKQAKHELQELRSLKENLTEQVEVLNVRVQQETQRKTLCQGDLKVQRQEINSLRSSEQQLKQELNHLLELKLTLEKQNQELSKEREESEKQLKEMKDQLEAEQYFTKLYKTQIRELKEESDEKVKLYKDAQQRIEDLQEERDSLASQLEVSLTKADSEQLARITVEEQYSDLEKEKIMKELEIKDMIARHRQDLAEKDGTINSLEESNRTLTVDVANLASEKEELNNKLKHIQQKLEKIKEEEKQMKSLTVSYEKQIQVEKTLKIQAINKLAEVMKKTDGRPHQINNTDIRRKEKEIRQLQLQLRTEKEKLNHSIIKYQREINDMQAMIAEESQVRLEMQMSLDSKDSDIERLRSQLTSLSIHSLDTTSISSIGNDLDSDEAYPVRITHSHTSESMSFTYQRSSKSVCIDTRPCRIAPRFSSDSDEEEDEEDEWRQTPALTYETPPDPEAAAAESGLEGWLSMPAKNTKRFGWDKKYVVVSSKKILFYDSEQDREQSSPFMILDIDKLFHVRPVTQTDVYRADPREIPRIFQILYANEGESKKEELPVESLSVTERSLCIPHKGHEFVLTLYHFPSSCEVCPRPLWHVFRPPPALECRRCRVKCHKDHIDRKEEVLAPCRVNYDMSTAKEMLLLANSTEEQKKWVSRLLKRVPRKPSIAHSSSIAISAAPSEATPTTLSPQPSPRLAQSSPRLSHRAAVKVHSTRQQPSSKNSPGPMLRIILH

Target ORF information:

RefSeq Version	XM_021468357.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468357.1

ORF Insert Sequence:

ATGTCGACCG GAGCGGGGAA GAGAATAGAG AGCCGGGTGA GAAAACTAGA GACGCTGATC 
AAGAACCAGA AATCACCGTT GAACTTGGAG AGTTTGCTGG ATTCAGTAAC TGCCCTTGCC 
CTTGATCTGA ACCATCCAGC ACTGAGAAAG AACAAAAACA TAGATGCCTT CTTAAGCAGA 
TATGAAAAAG CCGTGTGCAA CCTCAAGGAA CTACAGGTGA AGCTGGATGA TTTTGATCGA 
GTCAAGCTGA TCGGTCGTGG AGCGTTTGGC GCAGTGCAGC TGGTACGGCA CAAAGTATCT 
CAACAAGTTT ATGCCATGAA GCAGCTCAGC AAGTTTGAGA TGGTCAAGCG CTCTGACTCC 
GCCTTTTTCT GGGAGGAGCG TGACATCATG GCTTTCTCCC AGAGCCCTTG GATAGTTCAG 
CTGTGCTGTG CATTCCAAGA TGAAAAGTAC CTTTATTTGG TGATGGAGTT CATGCCAGGT 
GGAGATCTGG TGACTCTGAC CAGCAACTAT GACATCCCAG AGGAATGGGC TCAATTCTAT 
ACGGCAGAAG TGGTGCTGGC ACTCGACGCC ATCCATTCTC TGGGCTTCAT CCACAGAGAC 
ATCAAACCAG ACAACATGCT GCTGGACCGC AATGGACACC TCAAACTCGC AGACTTTGGC 
ACATGCATGA AGATGGACTC GACTGGTATG GTACGCTGTG ACACTGCTGT TGGGACACCT 
GATTACATTT CTCCAGAGGT TTTAATGTCT CAAGGAGGGA CTGGTTATTA CGGTCGCGAG 
TGTGACTGGT GGTCTGTTGG TGTTTTTATT TATGAGCTTC TAGTGGGCGA CACTCCATTT 
TATTCCGAGT CTCTGGTGGG CACATATGGA AAGATCATGG ATCACAAGAA CAGCTTAACT 
TTCCCAGATG ATATTGAAAT GTCAAAGAAT GCCAAAGACC TCATCTGTGC CTTTCTGAGT 
AGCAGGGAGG TGAGGCTTGG CCGAACTGGA GTGGATGAAA TAAAATGTCA CCCCTTCTTT 
AAGAATGATC AGTGGACCTT CGACACCATA CGAGACACCA TGGCTCCAGT GGTGCCAGAG 
CTGAGTAGCG ATATTGACAC CAGTAACTTT GATGATGACA TTAAGGATGA TCCGATAGGA 
ACAGAGACGT TCCCTCCGCC CCGTGCCTTC GCAGGGAACC AGCTACCATT TGTGGGTTTC 
ACCTATTTCA GGGAGGACCA ATTGTTAACA CAACAAAATA AGTGTTCAGA GGAAGATATC 
ATTGAAGATC ATGGCTTAAA CCACACAGAG TCTAACGACC TGGAGAAGAG GCTGAAAAAG 
TTGGAGGAAA AATTCAAACA TGAGATGCAA GCAAAGGAAG AACTGGAAAA TAAATGCCGA 
ATAGCAAATC AGCGGCTGGA GAAATTGTCC AAAGATCTTG AAGAAGAAGT GAATGCGAGA 
CAGGAGGCGG AAGACAACCT GAGATCCCTG GAGAAAGAGA AAGTTTTACT GAAACACCAG 
CGTACACAGA GCGTAAGAAA AGCTGGTCTA GAGACCGACA GAAAACGGCT GCTGGAGAAT 
GAAGTAAGCA GCCTGAAAGA GCAGCTTGCC GAACTGAAGA AAAAGAACCA GATCTCTCAT 
CTGTCTGCTG AGAAGAACAT TCACCTGGAA AGACAGCTGG AGGAGGTGAG TGCCAAGCTT 
CAGGCCGAGC TGGAGGAGTC GGAGAGGCTG AAGAAGGCCC AGATCGAGGC CTTTAGACAG 
TCCCAGCAGC TGGAGCTTTC CCTCAGGGAG CTGCAGGAGC GTCTGGCCCA GCTGGAGAAC 
AGCAGGCTGG TTCTGGAGCA GGACAAACTC AGCCTGCAGA CCTCGCTAGA GCTGGAGAAA 
CGAGAGAGAA ACGCCGGCTC AGAGACCATC ACTGACCTGC AGGGTCGTAT CTATGGACTG 
GAGGGTGAGT TGAAGCATAT AAAGAGTTCT CTATCAAAAG CTGAAGTTGA GAAAAGACAA 
TTACAAGAGG ATCTTACAAC CCTGGAAAAG GAGAAGAATA AGCAGGAGAT CGACCTCTCC 
TTCAAACTCA AAGCAGTTCA GCAGAGTCTG GAGCAGGAGG AGGCTGAACA TAAAACCACT 
AAAGCCAGAT TGGCCGACAA CAACAAAATC AACCAATCCA TAGAGGCAAA GTCTGAGACC 
CTGAAAGACA TGGAACATAA GCTGTTGGAA GAACGATCTG CGAAGCAACA ACTGGAAAAC 
AGACTGATGC AGCTGGAGAA GGAAAACTCT GTATTAGACT GTGATTACAA ACAAGCCAAA 
CATGAGCTTC AGGAGCTCCG CTCGCTCAAA GAAAATCTCA CAGAGCAGGT GGAGGTGCTG 
AATGTGCGGG TGCAGCAGGA GACACAGAGG AAAACTCTCT GTCAAGGTGA TCTCAAAGTT 
CAGAGGCAGG AGATCAACAG CCTGCGGTCA TCAGAACAAC AGCTCAAACA AGAACTCAAC 
CATCTGCTGG AGCTCAAACT CACACTGGAG AAACAAAACC AGGAGCTGTC CAAGGAACGA 
GAAGAATCTG AAAAACAGCT GAAGGAGATG AAGGACCAGC TGGAGGCTGA GCAGTACTTC 
ACGAAACTGT ACAAGACTCA GATTCGAGAG CTGAAAGAGG AAAGCGATGA AAAGGTTAAA 
CTCTACAAAG ATGCTCAGCA GAGAATAGAG GACCTGCAGG AAGAGAGGGA CTCGTTAGCG 
TCTCAGTTGG AGGTGAGCCT GACGAAGGCT GACTCTGAAC AGTTGGCTCG CATCACCGTT 
GAAGAGCAAT ACTCGGACCT GGAGAAAGAG AAGATCATGA AGGAACTGGA GATAAAGGAC 
ATGATCGCCA GACACCGGCA GGATCTTGCA GAAAAGGACG GTACCATCAA CTCGCTGGAG 
GAGTCAAACC GTACGCTGAC TGTGGATGTT GCCAACCTGG CCAGTGAGAA AGAGGAGCTC 
AACAATAAAC TGAAGCACAT ACAGCAAAAG CTGGAGAAGA TCAAAGAAGA AGAGAAACAG 
ATGAAATCTC TCACAGTGTC GTATGAGAAG CAGATACAGG TGGAGAAAAC ACTCAAGATT 
CAGGCTATAA ACAAGCTGGC GGAGGTCATG AAGAAGACGG ACGGCAGGCC ACACCAAATC 
AACAACACAG ACATCCGCAG GAAGGAGAAG GAGATCAGAC AACTCCAGCT GCAGCTCCGC 
ACGGAGAAAG AAAAGCTTAA CCACTCCATC ATCAAATACC AGAGAGAAAT CAACGACATG 
CAGGCTATGA TAGCAGAGGA GTCTCAGGTG CGTCTGGAGA TGCAGATGTC TCTGGACAGT 
AAAGACAGTG ATATTGAGCG TCTGCGCAGT CAACTCACGT CTCTGAGCAT TCACTCTCTG 
GACACCACCA GCATCAGCAG CATCGGCAAT GACCTGGATT CTGACGAGGC ATATCCAGTG 
CGAATCACAC ACTCACACAC CTCGGAGTCT ATGTCCTTCA CATACCAGCG AAGCTCCAAG 
TCGGTGTGTA TTGATACCCG GCCCTGTCGA ATCGCTCCTC GCTTCTCCTC TGACTCTGAT 
GAAGAGGAGG ATGAGGAAGA TGAGTGGAGA CAAACACCTG CCCTTACATA TGAGACGCCT 
CCTGACCCTG AGGCTGCAGC CGCAGAGTCC GGTCTTGAAG GATGGCTCTC AATGCCTGCC 
AAAAACACCA AACGGTTTGG ATGGGATAAG AAGTATGTGG TTGTGAGCAG CAAGAAAATC 
CTGTTTTATG ACTCTGAACA AGACCGAGAG CAGTCCAGTC CATTCATGAT CCTGGACATT 
GACAAACTCT TCCACGTGAG ACCCGTCACT CAGACAGATG TTTACCGCGC AGATCCCAGA 
GAAATCCCTC GCATCTTCCA GATACTATAT GCGAATGAAG GAGAGAGCAA GAAAGAGGAG 
CTGCCCGTGG AGTCGTTATC TGTGACTGAG AGGTCACTGT GTATCCCACA CAAGGGTCAC 
GAGTTTGTTC TGACGCTTTA TCACTTCCCC TCGAGCTGCG AGGTCTGTCC ACGACCCTTA 
TGGCACGTCT TCAGACCTCC GCCGGCTCTC GAGTGCAGAC GCTGTAGAGT CAAGTGCCAT 
AAAGACCACA TCGATCGCAA AGAAGAGGTC CTCGCTCCAT GTAGAGTGAA TTACGACATG 
TCCACAGCGA AAGAGATGCT GCTGCTGGCA AACTCCACAG AGGAGCAGAA GAAGTGGGTC 
AGTCGGCTGC TCAAGAGGGT TCCGCGTAAA CCTTCCATCG CCCACTCTTC CAGCATAGCC 
ATATCCGCTG CGCCCTCAGA GGCAACACCC ACTACCCTTT CCCCTCAGCC GTCGCCTCGC 
CTCGCTCAGT CTTCACCCAG ACTTTCACAC CGAGCAGCCG TCAAAGTGCA CTCTACACGA 
CAGCAGCCCT CCAGCAAGAA CAGCCCTGGC CCAATGCTCC GTATCATTCT TCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rock2b ( XM_021468358.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324033.1 Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X3, mRNA.

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CloneID	ODa150578
Clone ID Related Accession (Same CDS sequence)	XM_021468357.1 , XM_021468358.1
Accession Version	XM_021468358.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4437bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho-associated, coiled-coil containing protein kinase 2b isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGACCG GAGCGGGGAA GAGAATAGAG AGCCGGGTGA GAAAACTAGA GACGCTGATC 
AAGAACCAGA AATCACCGTT GAACTTGGAG AGTTTGCTGG ATTCAGTAAC TGCCCTTGCC 
CTTGATCTGA ACCATCCAGC ACTGAGAAAG AACAAAAACA TAGATGCCTT CTTAAGCAGA 
TATGAAAAAG CCGTGTGCAA CCTCAAGGAA CTACAGGTGA AGCTGGATGA TTTTGATCGA 
GTCAAGCTGA TCGGTCGTGG AGCGTTTGGC GCAGTGCAGC TGGTACGGCA CAAAGTATCT 
CAACAAGTTT ATGCCATGAA GCAGCTCAGC AAGTTTGAGA TGGTCAAGCG CTCTGACTCC 
GCCTTTTTCT GGGAGGAGCG TGACATCATG GCTTTCTCCC AGAGCCCTTG GATAGTTCAG 
CTGTGCTGTG CATTCCAAGA TGAAAAGTAC CTTTATTTGG TGATGGAGTT CATGCCAGGT 
GGAGATCTGG TGACTCTGAC CAGCAACTAT GACATCCCAG AGGAATGGGC TCAATTCTAT 
ACGGCAGAAG TGGTGCTGGC ACTCGACGCC ATCCATTCTC TGGGCTTCAT CCACAGAGAC 
ATCAAACCAG ACAACATGCT GCTGGACCGC AATGGACACC TCAAACTCGC AGACTTTGGC 
ACATGCATGA AGATGGACTC GACTGGTATG GTACGCTGTG ACACTGCTGT TGGGACACCT 
GATTACATTT CTCCAGAGGT TTTAATGTCT CAAGGAGGGA CTGGTTATTA CGGTCGCGAG 
TGTGACTGGT GGTCTGTTGG TGTTTTTATT TATGAGCTTC TAGTGGGCGA CACTCCATTT 
TATTCCGAGT CTCTGGTGGG CACATATGGA AAGATCATGG ATCACAAGAA CAGCTTAACT 
TTCCCAGATG ATATTGAAAT GTCAAAGAAT GCCAAAGACC TCATCTGTGC CTTTCTGAGT 
AGCAGGGAGG TGAGGCTTGG CCGAACTGGA GTGGATGAAA TAAAATGTCA CCCCTTCTTT 
AAGAATGATC AGTGGACCTT CGACACCATA CGAGACACCA TGGCTCCAGT GGTGCCAGAG 
CTGAGTAGCG ATATTGACAC CAGTAACTTT GATGATGACA TTAAGGATGA TCCGATAGGA 
ACAGAGACGT TCCCTCCGCC CCGTGCCTTC GCAGGGAACC AGCTACCATT TGTGGGTTTC 
ACCTATTTCA GGGAGGACCA ATTGTTAACA CAACAAAATA AGTGTTCAGA GGAAGATATC 
ATTGAAGATC ATGGCTTAAA CCACACAGAG TCTAACGACC TGGAGAAGAG GCTGAAAAAG 
TTGGAGGAAA AATTCAAACA TGAGATGCAA GCAAAGGAAG AACTGGAAAA TAAATGCCGA 
ATAGCAAATC AGCGGCTGGA GAAATTGTCC AAAGATCTTG AAGAAGAAGT GAATGCGAGA 
CAGGAGGCGG AAGACAACCT GAGATCCCTG GAGAAAGAGA AAGTTTTACT GAAACACCAG 
CGTACACAGA GCGTAAGAAA AGCTGGTCTA GAGACCGACA GAAAACGGCT GCTGGAGAAT 
GAAGTAAGCA GCCTGAAAGA GCAGCTTGCC GAACTGAAGA AAAAGAACCA GATCTCTCAT 
CTGTCTGCTG AGAAGAACAT TCACCTGGAA AGACAGCTGG AGGAGGTGAG TGCCAAGCTT 
CAGGCCGAGC TGGAGGAGTC GGAGAGGCTG AAGAAGGCCC AGATCGAGGC CTTTAGACAG 
TCCCAGCAGC TGGAGCTTTC CCTCAGGGAG CTGCAGGAGC GTCTGGCCCA GCTGGAGAAC 
AGCAGGCTGG TTCTGGAGCA GGACAAACTC AGCCTGCAGA CCTCGCTAGA GCTGGAGAAA 
CGAGAGAGAA ACGCCGGCTC AGAGACCATC ACTGACCTGC AGGGTCGTAT CTATGGACTG 
GAGGGTGAGT TGAAGCATAT AAAGAGTTCT CTATCAAAAG CTGAAGTTGA GAAAAGACAA 
TTACAAGAGG ATCTTACAAC CCTGGAAAAG GAGAAGAATA AGCAGGAGAT CGACCTCTCC 
TTCAAACTCA AAGCAGTTCA GCAGAGTCTG GAGCAGGAGG AGGCTGAACA TAAAACCACT 
AAAGCCAGAT TGGCCGACAA CAACAAAATC AACCAATCCA TAGAGGCAAA GTCTGAGACC 
CTGAAAGACA TGGAACATAA GCTGTTGGAA GAACGATCTG CGAAGCAACA ACTGGAAAAC 
AGACTGATGC AGCTGGAGAA GGAAAACTCT GTATTAGACT GTGATTACAA ACAAGCCAAA 
CATGAGCTTC AGGAGCTCCG CTCGCTCAAA GAAAATCTCA CAGAGCAGGT GGAGGTGCTG 
AATGTGCGGG TGCAGCAGGA GACACAGAGG AAAACTCTCT GTCAAGGTGA TCTCAAAGTT 
CAGAGGCAGG AGATCAACAG CCTGCGGTCA TCAGAACAAC AGCTCAAACA AGAACTCAAC 
CATCTGCTGG AGCTCAAACT CACACTGGAG AAACAAAACC AGGAGCTGTC CAAGGAACGA 
GAAGAATCTG AAAAACAGCT GAAGGAGATG AAGGACCAGC TGGAGGCTGA GCAGTACTTC 
ACGAAACTGT ACAAGACTCA GATTCGAGAG CTGAAAGAGG AAAGCGATGA AAAGGTTAAA 
CTCTACAAAG ATGCTCAGCA GAGAATAGAG GACCTGCAGG AAGAGAGGGA CTCGTTAGCG 
TCTCAGTTGG AGGTGAGCCT GACGAAGGCT GACTCTGAAC AGTTGGCTCG CATCACCGTT 
GAAGAGCAAT ACTCGGACCT GGAGAAAGAG AAGATCATGA AGGAACTGGA GATAAAGGAC 
ATGATCGCCA GACACCGGCA GGATCTTGCA GAAAAGGACG GTACCATCAA CTCGCTGGAG 
GAGTCAAACC GTACGCTGAC TGTGGATGTT GCCAACCTGG CCAGTGAGAA AGAGGAGCTC 
AACAATAAAC TGAAGCACAT ACAGCAAAAG CTGGAGAAGA TCAAAGAAGA AGAGAAACAG 
ATGAAATCTC TCACAGTGTC GTATGAGAAG CAGATACAGG TGGAGAAAAC ACTCAAGATT 
CAGGCTATAA ACAAGCTGGC GGAGGTCATG AAGAAGACGG ACGGCAGGCC ACACCAAATC 
AACAACACAG ACATCCGCAG GAAGGAGAAG GAGATCAGAC AACTCCAGCT GCAGCTCCGC 
ACGGAGAAAG AAAAGCTTAA CCACTCCATC ATCAAATACC AGAGAGAAAT CAACGACATG 
CAGGCTATGA TAGCAGAGGA GTCTCAGGTG CGTCTGGAGA TGCAGATGTC TCTGGACAGT 
AAAGACAGTG ATATTGAGCG TCTGCGCAGT CAACTCACGT CTCTGAGCAT TCACTCTCTG 
GACACCACCA GCATCAGCAG CATCGGCAAT GACCTGGATT CTGACGAGGC ATATCCAGTG 
CGAATCACAC ACTCACACAC CTCGGAGTCT ATGTCCTTCA CATACCAGCG AAGCTCCAAG 
TCGGTGTGTA TTGATACCCG GCCCTGTCGA ATCGCTCCTC GCTTCTCCTC TGACTCTGAT 
GAAGAGGAGG ATGAGGAAGA TGAGTGGAGA CAAACACCTG CCCTTACATA TGAGACGCCT 
CCTGACCCTG AGGCTGCAGC CGCAGAGTCC GGTCTTGAAG GATGGCTCTC AATGCCTGCC 
AAAAACACCA AACGGTTTGG ATGGGATAAG AAGTATGTGG TTGTGAGCAG CAAGAAAATC 
CTGTTTTATG ACTCTGAACA AGACCGAGAG CAGTCCAGTC CATTCATGAT CCTGGACATT 
GACAAACTCT TCCACGTGAG ACCCGTCACT CAGACAGATG TTTACCGCGC AGATCCCAGA 
GAAATCCCTC GCATCTTCCA GATACTATAT GCGAATGAAG GAGAGAGCAA GAAAGAGGAG 
CTGCCCGTGG AGTCGTTATC TGTGACTGAG AGGTCACTGT GTATCCCACA CAAGGGTCAC 
GAGTTTGTTC TGACGCTTTA TCACTTCCCC TCGAGCTGCG AGGTCTGTCC ACGACCCTTA 
TGGCACGTCT TCAGACCTCC GCCGGCTCTC GAGTGCAGAC GCTGTAGAGT CAAGTGCCAT 
AAAGACCACA TCGATCGCAA AGAAGAGGTC CTCGCTCCAT GTAGAGTGAA TTACGACATG 
TCCACAGCGA AAGAGATGCT GCTGCTGGCA AACTCCACAG AGGAGCAGAA GAAGTGGGTC 
AGTCGGCTGC TCAAGAGGGT TCCGCGTAAA CCTTCCATCG CCCACTCTTC CAGCATAGCC 
ATATCCGCTG CGCCCTCAGA GGCAACACCC ACTACCCTTT CCCCTCAGCC GTCGCCTCGC 
CTCGCTCAGT CTTCACCCAG ACTTTCACAC CGAGCAGCCG TCAAAGTGCA CTCTACACGA 
CAGCAGCCCT CCAGCAAGAA CAGCCCTGGC CCAATGCTCC GTATCATTCT TCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324033.1
CDS	475..4911
Translation	MSTGAGKRIESRVRKLETLIKNQKSPLNLESLLDSVTALALDLNHPALRKNKNIDAFLSRYEKAVCNLKELQVKLDDFDRVKLIGRGAFGAVQLVRHKVSQQVYAMKQLSKFEMVKRSDSAFFWEERDIMAFSQSPWIVQLCCAFQDEKYLYLVMEFMPGGDLVTLTSNYDIPEEWAQFYTAEVVLALDAIHSLGFIHRDIKPDNMLLDRNGHLKLADFGTCMKMDSTGMVRCDTAVGTPDYISPEVLMSQGGTGYYGRECDWWSVGVFIYELLVGDTPFYSESLVGTYGKIMDHKNSLTFPDDIEMSKNAKDLICAFLSSREVRLGRTGVDEIKCHPFFKNDQWTFDTIRDTMAPVVPELSSDIDTSNFDDDIKDDPIGTETFPPPRAFAGNQLPFVGFTYFREDQLLTQQNKCSEEDIIEDHGLNHTESNDLEKRLKKLEEKFKHEMQAKEELENKCRIANQRLEKLSKDLEEEVNARQEAEDNLRSLEKEKVLLKHQRTQSVRKAGLETDRKRLLENEVSSLKEQLAELKKKNQISHLSAEKNIHLERQLEEVSAKLQAELEESERLKKAQIEAFRQSQQLELSLRELQERLAQLENSRLVLEQDKLSLQTSLELEKRERNAGSETITDLQGRIYGLEGELKHIKSSLSKAEVEKRQLQEDLTTLEKEKNKQEIDLSFKLKAVQQSLEQEEAEHKTTKARLADNNKINQSIEAKSETLKDMEHKLLEERSAKQQLENRLMQLEKENSVLDCDYKQAKHELQELRSLKENLTEQVEVLNVRVQQETQRKTLCQGDLKVQRQEINSLRSSEQQLKQELNHLLELKLTLEKQNQELSKEREESEKQLKEMKDQLEAEQYFTKLYKTQIRELKEESDEKVKLYKDAQQRIEDLQEERDSLASQLEVSLTKADSEQLARITVEEQYSDLEKEKIMKELEIKDMIARHRQDLAEKDGTINSLEESNRTLTVDVANLASEKEELNNKLKHIQQKLEKIKEEEKQMKSLTVSYEKQIQVEKTLKIQAINKLAEVMKKTDGRPHQINNTDIRRKEKEIRQLQLQLRTEKEKLNHSIIKYQREINDMQAMIAEESQVRLEMQMSLDSKDSDIERLRSQLTSLSIHSLDTTSISSIGNDLDSDEAYPVRITHSHTSESMSFTYQRSSKSVCIDTRPCRIAPRFSSDSDEEEDEEDEWRQTPALTYETPPDPEAAAAESGLEGWLSMPAKNTKRFGWDKKYVVVSSKKILFYDSEQDREQSSPFMILDIDKLFHVRPVTQTDVYRADPREIPRIFQILYANEGESKKEELPVESLSVTERSLCIPHKGHEFVLTLYHFPSSCEVCPRPLWHVFRPPPALECRRCRVKCHKDHIDRKEEVLAPCRVNYDMSTAKEMLLLANSTEEQKKWVSRLLKRVPRKPSIAHSSSIAISAAPSEATPTTLSPQPSPRLAQSSPRLSHRAAVKVHSTRQQPSSKNSPGPMLRIILH

Target ORF information:

RefSeq Version	XM_021468358.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468358.1

ORF Insert Sequence:

ATGTCGACCG GAGCGGGGAA GAGAATAGAG AGCCGGGTGA GAAAACTAGA GACGCTGATC 
AAGAACCAGA AATCACCGTT GAACTTGGAG AGTTTGCTGG ATTCAGTAAC TGCCCTTGCC 
CTTGATCTGA ACCATCCAGC ACTGAGAAAG AACAAAAACA TAGATGCCTT CTTAAGCAGA 
TATGAAAAAG CCGTGTGCAA CCTCAAGGAA CTACAGGTGA AGCTGGATGA TTTTGATCGA 
GTCAAGCTGA TCGGTCGTGG AGCGTTTGGC GCAGTGCAGC TGGTACGGCA CAAAGTATCT 
CAACAAGTTT ATGCCATGAA GCAGCTCAGC AAGTTTGAGA TGGTCAAGCG CTCTGACTCC 
GCCTTTTTCT GGGAGGAGCG TGACATCATG GCTTTCTCCC AGAGCCCTTG GATAGTTCAG 
CTGTGCTGTG CATTCCAAGA TGAAAAGTAC CTTTATTTGG TGATGGAGTT CATGCCAGGT 
GGAGATCTGG TGACTCTGAC CAGCAACTAT GACATCCCAG AGGAATGGGC TCAATTCTAT 
ACGGCAGAAG TGGTGCTGGC ACTCGACGCC ATCCATTCTC TGGGCTTCAT CCACAGAGAC 
ATCAAACCAG ACAACATGCT GCTGGACCGC AATGGACACC TCAAACTCGC AGACTTTGGC 
ACATGCATGA AGATGGACTC GACTGGTATG GTACGCTGTG ACACTGCTGT TGGGACACCT 
GATTACATTT CTCCAGAGGT TTTAATGTCT CAAGGAGGGA CTGGTTATTA CGGTCGCGAG 
TGTGACTGGT GGTCTGTTGG TGTTTTTATT TATGAGCTTC TAGTGGGCGA CACTCCATTT 
TATTCCGAGT CTCTGGTGGG CACATATGGA AAGATCATGG ATCACAAGAA CAGCTTAACT 
TTCCCAGATG ATATTGAAAT GTCAAAGAAT GCCAAAGACC TCATCTGTGC CTTTCTGAGT 
AGCAGGGAGG TGAGGCTTGG CCGAACTGGA GTGGATGAAA TAAAATGTCA CCCCTTCTTT 
AAGAATGATC AGTGGACCTT CGACACCATA CGAGACACCA TGGCTCCAGT GGTGCCAGAG 
CTGAGTAGCG ATATTGACAC CAGTAACTTT GATGATGACA TTAAGGATGA TCCGATAGGA 
ACAGAGACGT TCCCTCCGCC CCGTGCCTTC GCAGGGAACC AGCTACCATT TGTGGGTTTC 
ACCTATTTCA GGGAGGACCA ATTGTTAACA CAACAAAATA AGTGTTCAGA GGAAGATATC 
ATTGAAGATC ATGGCTTAAA CCACACAGAG TCTAACGACC TGGAGAAGAG GCTGAAAAAG 
TTGGAGGAAA AATTCAAACA TGAGATGCAA GCAAAGGAAG AACTGGAAAA TAAATGCCGA 
ATAGCAAATC AGCGGCTGGA GAAATTGTCC AAAGATCTTG AAGAAGAAGT GAATGCGAGA 
CAGGAGGCGG AAGACAACCT GAGATCCCTG GAGAAAGAGA AAGTTTTACT GAAACACCAG 
CGTACACAGA GCGTAAGAAA AGCTGGTCTA GAGACCGACA GAAAACGGCT GCTGGAGAAT 
GAAGTAAGCA GCCTGAAAGA GCAGCTTGCC GAACTGAAGA AAAAGAACCA GATCTCTCAT 
CTGTCTGCTG AGAAGAACAT TCACCTGGAA AGACAGCTGG AGGAGGTGAG TGCCAAGCTT 
CAGGCCGAGC TGGAGGAGTC GGAGAGGCTG AAGAAGGCCC AGATCGAGGC CTTTAGACAG 
TCCCAGCAGC TGGAGCTTTC CCTCAGGGAG CTGCAGGAGC GTCTGGCCCA GCTGGAGAAC 
AGCAGGCTGG TTCTGGAGCA GGACAAACTC AGCCTGCAGA CCTCGCTAGA GCTGGAGAAA 
CGAGAGAGAA ACGCCGGCTC AGAGACCATC ACTGACCTGC AGGGTCGTAT CTATGGACTG 
GAGGGTGAGT TGAAGCATAT AAAGAGTTCT CTATCAAAAG CTGAAGTTGA GAAAAGACAA 
TTACAAGAGG ATCTTACAAC CCTGGAAAAG GAGAAGAATA AGCAGGAGAT CGACCTCTCC 
TTCAAACTCA AAGCAGTTCA GCAGAGTCTG GAGCAGGAGG AGGCTGAACA TAAAACCACT 
AAAGCCAGAT TGGCCGACAA CAACAAAATC AACCAATCCA TAGAGGCAAA GTCTGAGACC 
CTGAAAGACA TGGAACATAA GCTGTTGGAA GAACGATCTG CGAAGCAACA ACTGGAAAAC 
AGACTGATGC AGCTGGAGAA GGAAAACTCT GTATTAGACT GTGATTACAA ACAAGCCAAA 
CATGAGCTTC AGGAGCTCCG CTCGCTCAAA GAAAATCTCA CAGAGCAGGT GGAGGTGCTG 
AATGTGCGGG TGCAGCAGGA GACACAGAGG AAAACTCTCT GTCAAGGTGA TCTCAAAGTT 
CAGAGGCAGG AGATCAACAG CCTGCGGTCA TCAGAACAAC AGCTCAAACA AGAACTCAAC 
CATCTGCTGG AGCTCAAACT CACACTGGAG AAACAAAACC AGGAGCTGTC CAAGGAACGA 
GAAGAATCTG AAAAACAGCT GAAGGAGATG AAGGACCAGC TGGAGGCTGA GCAGTACTTC 
ACGAAACTGT ACAAGACTCA GATTCGAGAG CTGAAAGAGG AAAGCGATGA AAAGGTTAAA 
CTCTACAAAG ATGCTCAGCA GAGAATAGAG GACCTGCAGG AAGAGAGGGA CTCGTTAGCG 
TCTCAGTTGG AGGTGAGCCT GACGAAGGCT GACTCTGAAC AGTTGGCTCG CATCACCGTT 
GAAGAGCAAT ACTCGGACCT GGAGAAAGAG AAGATCATGA AGGAACTGGA GATAAAGGAC 
ATGATCGCCA GACACCGGCA GGATCTTGCA GAAAAGGACG GTACCATCAA CTCGCTGGAG 
GAGTCAAACC GTACGCTGAC TGTGGATGTT GCCAACCTGG CCAGTGAGAA AGAGGAGCTC 
AACAATAAAC TGAAGCACAT ACAGCAAAAG CTGGAGAAGA TCAAAGAAGA AGAGAAACAG 
ATGAAATCTC TCACAGTGTC GTATGAGAAG CAGATACAGG TGGAGAAAAC ACTCAAGATT 
CAGGCTATAA ACAAGCTGGC GGAGGTCATG AAGAAGACGG ACGGCAGGCC ACACCAAATC 
AACAACACAG ACATCCGCAG GAAGGAGAAG GAGATCAGAC AACTCCAGCT GCAGCTCCGC 
ACGGAGAAAG AAAAGCTTAA CCACTCCATC ATCAAATACC AGAGAGAAAT CAACGACATG 
CAGGCTATGA TAGCAGAGGA GTCTCAGGTG CGTCTGGAGA TGCAGATGTC TCTGGACAGT 
AAAGACAGTG ATATTGAGCG TCTGCGCAGT CAACTCACGT CTCTGAGCAT TCACTCTCTG 
GACACCACCA GCATCAGCAG CATCGGCAAT GACCTGGATT CTGACGAGGC ATATCCAGTG 
CGAATCACAC ACTCACACAC CTCGGAGTCT ATGTCCTTCA CATACCAGCG AAGCTCCAAG 
TCGGTGTGTA TTGATACCCG GCCCTGTCGA ATCGCTCCTC GCTTCTCCTC TGACTCTGAT 
GAAGAGGAGG ATGAGGAAGA TGAGTGGAGA CAAACACCTG CCCTTACATA TGAGACGCCT 
CCTGACCCTG AGGCTGCAGC CGCAGAGTCC GGTCTTGAAG GATGGCTCTC AATGCCTGCC 
AAAAACACCA AACGGTTTGG ATGGGATAAG AAGTATGTGG TTGTGAGCAG CAAGAAAATC 
CTGTTTTATG ACTCTGAACA AGACCGAGAG CAGTCCAGTC CATTCATGAT CCTGGACATT 
GACAAACTCT TCCACGTGAG ACCCGTCACT CAGACAGATG TTTACCGCGC AGATCCCAGA 
GAAATCCCTC GCATCTTCCA GATACTATAT GCGAATGAAG GAGAGAGCAA GAAAGAGGAG 
CTGCCCGTGG AGTCGTTATC TGTGACTGAG AGGTCACTGT GTATCCCACA CAAGGGTCAC 
GAGTTTGTTC TGACGCTTTA TCACTTCCCC TCGAGCTGCG AGGTCTGTCC ACGACCCTTA 
TGGCACGTCT TCAGACCTCC GCCGGCTCTC GAGTGCAGAC GCTGTAGAGT CAAGTGCCAT 
AAAGACCACA TCGATCGCAA AGAAGAGGTC CTCGCTCCAT GTAGAGTGAA TTACGACATG 
TCCACAGCGA AAGAGATGCT GCTGCTGGCA AACTCCACAG AGGAGCAGAA GAAGTGGGTC 
AGTCGGCTGC TCAAGAGGGT TCCGCGTAAA CCTTCCATCG CCCACTCTTC CAGCATAGCC 
ATATCCGCTG CGCCCTCAGA GGCAACACCC ACTACCCTTT CCCCTCAGCC GTCGCCTCGC 
CTCGCTCAGT CTTCACCCAG ACTTTCACAC CGAGCAGCCG TCAAAGTGCA CTCTACACGA 
CAGCAGCCCT CCAGCAAGAA CAGCCCTGGC CCAATGCTCC GTATCATTCT TCACTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rock2b ( XM_021468359.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324034.1 Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X4, mRNA.

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CloneID	ODa150579
Clone ID Related Accession (Same CDS sequence)	XM_021468359.1
Accession Version	XM_021468359.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4416bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho-associated, coiled-coil containing protein kinase 2b isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGACCG GAGCGGGGAA GAGAATAGAG AGCCGGGTGA GAAAACTAGA GACGCTGATC 
AAGAACCAGA AATCACCGTT GAACTTGGAG AGTTTGCTGG ATTCAGTAAC TGCCCTTGCC 
CTTGATCTGA ACCATCCAGC ACTGAGAAAG AACAAAAACA TAGATGCCTT CTTAAGCAGA 
TATGAAAAAG CCGTGTGCAA CCTCAAGGAA CTACAGGTGA AGCTGGATGA TTTTGATCGA 
GTCAAGCTGA TCGGTCGTGG AGCGTTTGGC GCAGTGCAGC TGGTACGGCA CAAAGTATCT 
CAACAAGTTT ATGCCATGAA GCAGCTCAGC AAGTTTGAGA TGGTCAAGCG CTCTGACTCC 
GCCTTTTTCT GGGAGGAGCG TGACATCATG GCTTTCTCCC AGAGCCCTTG GATAGTTCAG 
CTGTGCTGTG CATTCCAAGA TGAAAAGTAC CTTTATTTGG TGATGGAGTT CATGCCAGGT 
GGAGATCTGG TGACTCTGAC CAGCAACTAT GACATCCCAG AGGAATGGGC TCAATTCTAT 
ACGGCAGAAG TGGTGCTGGC ACTCGACGCC ATCCATTCTC TGGGCTTCAT CCACAGAGAC 
ATCAAACCAG ACAACATGCT GCTGGACCGC AATGGACACC TCAAACTCGC AGACTTTGGC 
ACATGCATGA AGATGGACTC GACTGGTATG GTACGCTGTG ACACTGCTGT TGGGACACCT 
GATTACATTT CTCCAGAGGT TTTAATGTCT CAAGGAGGGA CTGGTTATTA CGGTCGCGAG 
TGTGACTGGT GGTCTGTTGG TGTTTTTATT TATGAGCTTC TAGTGGGCGA CACTCCATTT 
TATTCCGAGT CTCTGGTGGG CACATATGGA AAGATCATGG ATCACAAGAA CAGCTTAACT 
TTCCCAGATG ATATTGAAAT GTCAAAGAAT GCCAAAGACC TCATCTGTGC CTTTCTGAGT 
AGCAGGGAGG TGAGGCTTGG CCGAACTGGA GTGGATGAAA TAAAATGTCA CCCCTTCTTT 
AAGAATGATC AGTGGACCTT CGACACCATA CGAGACACCA TGGCTCCAGT GGTGCCAGAG 
CTGAGTAGCG ATATTGACAC CAGTAACTTT GATGATGACA TTAAGGATGA TCCGATAGGA 
ACAGAGACGT TCCCTCCGCC CCGTGCCTTC GCAGGGAACC AGCTACCATT TGTGGGTTTC 
ACCTATTTCA GGGAGGACCA ATTGTTAACA CAACAAAATA AGTGTTCAGA GGAAGATATC 
ATTGAAGATC ATGGCTTAAA CCACACAGAG TCTAACGACC TGGAGAAGAG GCTGAAAAAG 
TTGGAGGAAA AATTCAAACA TGAGATGCAA GCAAAGGAAG AACTGGAAAA TAAATGCCGA 
ATAGCAAATC AGCGGCTGGA GAAATTGTCC AAAGATCTTG AAGAAGAAGT GAATGCGAGA 
CAGGAGGCGG AAGACAACCT GAGATCCCTG GAGAAAGAGA AAGTTTTACT GAAACACCAG 
CGTACACAGA GCGTAAGAAA AGCTGGTCTA GAGACCGACA GAAAACGGCT GCTGGAGAAT 
GAAGTAAGCA GCCTGAAAGA GCAGCTTGCC GAACTGAAGA AAAAGAACCA GATCTCTCAT 
CTGTCTGCTG AGAAGAACAT TCACCTGGAA AGACAGCTGG AGGAGGTGAG TGCCAAGCTT 
CAGGCCGAGC TGGAGGAGTC GGAGAGGCTG AAGAAGGCCC AGATCGAGGC CTTTAGACAG 
TCCCAGCAGC TGGAGCTTTC CCTCAGGGAG CTGCAGGAGC GTCTGGCCCA GCTGGAGAAC 
AGCAGGCTGG TTCTGGAGCA GGACAAACTC AGCCTGCAGA CCTCGCTAGA GCTGGAGAAA 
CGAGAGAGAA ACGCCGGCTC AGAGACCATC ACTGACCTGC AGGGTCGTAT CTATGGACTG 
GAGGGTGAGT TGAAGCATAT AAAGAGTTCT CTATCAAAAG CTGAAGTTGA GAAAAGACAA 
TTACAAGAGG ATCTTACAAC CCTGGAAAAG GAGAAGAATA AGCAGGAGAT CGACCTCTCC 
TTCAAACTCA AAGCAGTTCA GCAGAGTCTG GAGCAGGAGG AGGCTGAACA TAAAACCACT 
AAAGCCAGAT TGGCCGACAA CAACAAAATC AACCAATCCA TAGAGGCAAA GTCTGAGACC 
CTGAAAGACA TGGAACATAA GCTGTTGGAA GAACGATCTG CGAAGCAACA ACTGGAAAAC 
AGACTGATGC AGCTGGAGAA GGAAAACTCT GTATTAGACT GTGATTACAA ACAAGCCAAA 
CATGAGCTTC AGGAGCTCCG CTCGCTCAAA GAAAATCTCA CAGAGCAGGT GGAGGTGCTG 
AATGTGCGGG TGCAGCAGGA GACACAGAGG AAAACTCTCT GTCAAGGTGA TCTCAAAGTT 
CAGAGGCAGG AGATCAACAG CCTGCGGTCA TCAGAACAAC AGCTCAAACA AGAACTCAAC 
CATCTGCTGG AGCTCAAACT CACACTGGAG AAACAAAACC AGGAGCTGTC CAAGGAACGA 
GAAGAATCTG AAAAACAGCT GAAGGAGATG AAGGACCAGC TGGAGGCTGA GCAGTACTTC 
ACGAAACTGT ACAAGACTCA GATTCGAGAG CTGAAAGAGG AAAGCGATGA AAAGGTTAAA 
CTCTACAAAG ATGCTCAGCA GAGAATAGAG GACCTGCAGG AAGAGAGGGA CTCGTTAGCG 
TCTCAGTTGG AGGTGAGCCT GACGAAGGCT GACTCTGAAC AGTTGGCTCG CATCACCGTT 
GAAGAGCAAT ACTCGGACCT GGAGAAAGAG AAGATCATGA AGGAACTGGA GATAAAGGAC 
ATGATCGCCA GACACCGGCA GGATCTTGCA GAAAAGGACG GTACCATCAA CTCGCTGGAG 
GAGTCAAACC GTACGCTGAC TGTGGATGTT GCCAACCTGG CCAGTGAGAA AGAGGAGCTC 
AACAATAAAC TGAAGCACAT ACAGCAAAAG CTGGAGAAGA TCAAAGAAGA AGAGAAACAG 
ATGAAATCTC TCACAGTGTC GTATGAGAAG CAGATACAGG TGGAGAAAAC ACTCAAGATT 
CAGGCTATAA ACAAGCTGGC GGAGGTCATG AAGAAGACGG ACGGCAGGCC ACACCAAATC 
AACAACACAG ACATCCGCAG GAAGGAGAAG GAGATCAGAC AACTCCAGCT GCAGCTCCGC 
ACGGAGAAAG AAAAGCTTAA CCACTCCATC ATCAAATACC AGAGAGAAAT CAACGACATG 
CAGGCTATGA TAGCAGAGGA GTCTCAGGTG CGTCTGGAGA TGCAGATGTC TCTGGACAGT 
AAAGACAGTG ATATTGAGCG TCTGCGCAGT CAACTCACGT CTCTGAGCAT TCACTCTCTG 
GACACCACCA GCATCAGCAG CATCGGCAAT GACCTGGATT CTGACGAGGC ATATCCAGTG 
CGAATCACAC ACTCACACAC CTCGGAGTCT ATGTCCTTCA CATACCAGCG AAGCTCCAAG 
TCGGTGTGTA TTGATACCCG GCCCTGTCGA ATCGCTCCTC GCTTCTCCTC TGACTCTGAT 
GAAGAGGAGG ATGAGGAAGA TGAGTGGAGA CAAACACCTG CCCTTACATA TGAGACGCCT 
CCTGACCCTG AGGCTGCAGC CGCAGAGTCC GGTCTTGAAG GATGGCTCTC AATGCCTGCC 
AAAAACACCA AACGGTTTGG ATGGGATAAG AAGTATGTGG TTGTGAGCAG CAAGAAAATC 
CTGTTTTATG ACTCTGAACA AGACCGAGAG CAGTCCAGTC CATTCATGAT CCTGGACATT 
GACAAACTCT TCCACGTGAG ACCCGTCACT CAGACAGATG TTTACCGCGC AGATCCCAGA 
GAAATCCCTC GCATCTTCCA GATACTATAT GCGAATGAAG GAGAGAGCAA GAAAGAGGAG 
CTGCCCGTGG AGTCGTTATC TGTGACTGAG AGGTCACTGT GTATCCCACA CAAGGGTCAC 
GAGTTTGTTC TGACGCTTTA TCACTTCCCC TCGAGCTGCG AGGTCTGTCC ACGACCCTTA 
TGGCACGTCT TCAGACCTCC GCCGGCTCTC GAGTGCAGAC GCTGTAGAGT CAAGTGCCAT 
AAAGACCACA TCGATCGCAA AGAAGAGGTC CTCGCTCCAT GTAGAGTGAA TTACGACATG 
TCCACAGCGA AAGAGATGCT GCTGCTGGCA AACTCCACAG AGGAGCAGAA GAAGTGGGTC 
AGTCGGCTGC TCAAGAGGGT TCCGCGTAAA CCTTCCATCG CCCACTCTTC CAGCATAGCC 
ATATCCGCTG CGCCCTCAGA GGCAACACCC ACTACCCTTT CCCCTCAGCC GTCGCCTCGC 
CTCGCTCAGT CTTCACCCAG ACTTTCACAC CGAGCAGCCG TCAAAGTGCA CTCTACACGA 
CAGCAGCCCT CCAGCAAGAA CAGACTGGTT TGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324034.1
CDS	475..4890
Translation	MSTGAGKRIESRVRKLETLIKNQKSPLNLESLLDSVTALALDLNHPALRKNKNIDAFLSRYEKAVCNLKELQVKLDDFDRVKLIGRGAFGAVQLVRHKVSQQVYAMKQLSKFEMVKRSDSAFFWEERDIMAFSQSPWIVQLCCAFQDEKYLYLVMEFMPGGDLVTLTSNYDIPEEWAQFYTAEVVLALDAIHSLGFIHRDIKPDNMLLDRNGHLKLADFGTCMKMDSTGMVRCDTAVGTPDYISPEVLMSQGGTGYYGRECDWWSVGVFIYELLVGDTPFYSESLVGTYGKIMDHKNSLTFPDDIEMSKNAKDLICAFLSSREVRLGRTGVDEIKCHPFFKNDQWTFDTIRDTMAPVVPELSSDIDTSNFDDDIKDDPIGTETFPPPRAFAGNQLPFVGFTYFREDQLLTQQNKCSEEDIIEDHGLNHTESNDLEKRLKKLEEKFKHEMQAKEELENKCRIANQRLEKLSKDLEEEVNARQEAEDNLRSLEKEKVLLKHQRTQSVRKAGLETDRKRLLENEVSSLKEQLAELKKKNQISHLSAEKNIHLERQLEEVSAKLQAELEESERLKKAQIEAFRQSQQLELSLRELQERLAQLENSRLVLEQDKLSLQTSLELEKRERNAGSETITDLQGRIYGLEGELKHIKSSLSKAEVEKRQLQEDLTTLEKEKNKQEIDLSFKLKAVQQSLEQEEAEHKTTKARLADNNKINQSIEAKSETLKDMEHKLLEERSAKQQLENRLMQLEKENSVLDCDYKQAKHELQELRSLKENLTEQVEVLNVRVQQETQRKTLCQGDLKVQRQEINSLRSSEQQLKQELNHLLELKLTLEKQNQELSKEREESEKQLKEMKDQLEAEQYFTKLYKTQIRELKEESDEKVKLYKDAQQRIEDLQEERDSLASQLEVSLTKADSEQLARITVEEQYSDLEKEKIMKELEIKDMIARHRQDLAEKDGTINSLEESNRTLTVDVANLASEKEELNNKLKHIQQKLEKIKEEEKQMKSLTVSYEKQIQVEKTLKIQAINKLAEVMKKTDGRPHQINNTDIRRKEKEIRQLQLQLRTEKEKLNHSIIKYQREINDMQAMIAEESQVRLEMQMSLDSKDSDIERLRSQLTSLSIHSLDTTSISSIGNDLDSDEAYPVRITHSHTSESMSFTYQRSSKSVCIDTRPCRIAPRFSSDSDEEEDEEDEWRQTPALTYETPPDPEAAAAESGLEGWLSMPAKNTKRFGWDKKYVVVSSKKILFYDSEQDREQSSPFMILDIDKLFHVRPVTQTDVYRADPREIPRIFQILYANEGESKKEELPVESLSVTERSLCIPHKGHEFVLTLYHFPSSCEVCPRPLWHVFRPPPALECRRCRVKCHKDHIDRKEEVLAPCRVNYDMSTAKEMLLLANSTEEQKKWVSRLLKRVPRKPSIAHSSSIAISAAPSEATPTTLSPQPSPRLAQSSPRLSHRAAVKVHSTRQQPSSKNRLVW

Target ORF information:

RefSeq Version	XM_021468359.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468359.1

ORF Insert Sequence:

ATGTCGACCG GAGCGGGGAA GAGAATAGAG AGCCGGGTGA GAAAACTAGA GACGCTGATC 
AAGAACCAGA AATCACCGTT GAACTTGGAG AGTTTGCTGG ATTCAGTAAC TGCCCTTGCC 
CTTGATCTGA ACCATCCAGC ACTGAGAAAG AACAAAAACA TAGATGCCTT CTTAAGCAGA 
TATGAAAAAG CCGTGTGCAA CCTCAAGGAA CTACAGGTGA AGCTGGATGA TTTTGATCGA 
GTCAAGCTGA TCGGTCGTGG AGCGTTTGGC GCAGTGCAGC TGGTACGGCA CAAAGTATCT 
CAACAAGTTT ATGCCATGAA GCAGCTCAGC AAGTTTGAGA TGGTCAAGCG CTCTGACTCC 
GCCTTTTTCT GGGAGGAGCG TGACATCATG GCTTTCTCCC AGAGCCCTTG GATAGTTCAG 
CTGTGCTGTG CATTCCAAGA TGAAAAGTAC CTTTATTTGG TGATGGAGTT CATGCCAGGT 
GGAGATCTGG TGACTCTGAC CAGCAACTAT GACATCCCAG AGGAATGGGC TCAATTCTAT 
ACGGCAGAAG TGGTGCTGGC ACTCGACGCC ATCCATTCTC TGGGCTTCAT CCACAGAGAC 
ATCAAACCAG ACAACATGCT GCTGGACCGC AATGGACACC TCAAACTCGC AGACTTTGGC 
ACATGCATGA AGATGGACTC GACTGGTATG GTACGCTGTG ACACTGCTGT TGGGACACCT 
GATTACATTT CTCCAGAGGT TTTAATGTCT CAAGGAGGGA CTGGTTATTA CGGTCGCGAG 
TGTGACTGGT GGTCTGTTGG TGTTTTTATT TATGAGCTTC TAGTGGGCGA CACTCCATTT 
TATTCCGAGT CTCTGGTGGG CACATATGGA AAGATCATGG ATCACAAGAA CAGCTTAACT 
TTCCCAGATG ATATTGAAAT GTCAAAGAAT GCCAAAGACC TCATCTGTGC CTTTCTGAGT 
AGCAGGGAGG TGAGGCTTGG CCGAACTGGA GTGGATGAAA TAAAATGTCA CCCCTTCTTT 
AAGAATGATC AGTGGACCTT CGACACCATA CGAGACACCA TGGCTCCAGT GGTGCCAGAG 
CTGAGTAGCG ATATTGACAC CAGTAACTTT GATGATGACA TTAAGGATGA TCCGATAGGA 
ACAGAGACGT TCCCTCCGCC CCGTGCCTTC GCAGGGAACC AGCTACCATT TGTGGGTTTC 
ACCTATTTCA GGGAGGACCA ATTGTTAACA CAACAAAATA AGTGTTCAGA GGAAGATATC 
ATTGAAGATC ATGGCTTAAA CCACACAGAG TCTAACGACC TGGAGAAGAG GCTGAAAAAG 
TTGGAGGAAA AATTCAAACA TGAGATGCAA GCAAAGGAAG AACTGGAAAA TAAATGCCGA 
ATAGCAAATC AGCGGCTGGA GAAATTGTCC AAAGATCTTG AAGAAGAAGT GAATGCGAGA 
CAGGAGGCGG AAGACAACCT GAGATCCCTG GAGAAAGAGA AAGTTTTACT GAAACACCAG 
CGTACACAGA GCGTAAGAAA AGCTGGTCTA GAGACCGACA GAAAACGGCT GCTGGAGAAT 
GAAGTAAGCA GCCTGAAAGA GCAGCTTGCC GAACTGAAGA AAAAGAACCA GATCTCTCAT 
CTGTCTGCTG AGAAGAACAT TCACCTGGAA AGACAGCTGG AGGAGGTGAG TGCCAAGCTT 
CAGGCCGAGC TGGAGGAGTC GGAGAGGCTG AAGAAGGCCC AGATCGAGGC CTTTAGACAG 
TCCCAGCAGC TGGAGCTTTC CCTCAGGGAG CTGCAGGAGC GTCTGGCCCA GCTGGAGAAC 
AGCAGGCTGG TTCTGGAGCA GGACAAACTC AGCCTGCAGA CCTCGCTAGA GCTGGAGAAA 
CGAGAGAGAA ACGCCGGCTC AGAGACCATC ACTGACCTGC AGGGTCGTAT CTATGGACTG 
GAGGGTGAGT TGAAGCATAT AAAGAGTTCT CTATCAAAAG CTGAAGTTGA GAAAAGACAA 
TTACAAGAGG ATCTTACAAC CCTGGAAAAG GAGAAGAATA AGCAGGAGAT CGACCTCTCC 
TTCAAACTCA AAGCAGTTCA GCAGAGTCTG GAGCAGGAGG AGGCTGAACA TAAAACCACT 
AAAGCCAGAT TGGCCGACAA CAACAAAATC AACCAATCCA TAGAGGCAAA GTCTGAGACC 
CTGAAAGACA TGGAACATAA GCTGTTGGAA GAACGATCTG CGAAGCAACA ACTGGAAAAC 
AGACTGATGC AGCTGGAGAA GGAAAACTCT GTATTAGACT GTGATTACAA ACAAGCCAAA 
CATGAGCTTC AGGAGCTCCG CTCGCTCAAA GAAAATCTCA CAGAGCAGGT GGAGGTGCTG 
AATGTGCGGG TGCAGCAGGA GACACAGAGG AAAACTCTCT GTCAAGGTGA TCTCAAAGTT 
CAGAGGCAGG AGATCAACAG CCTGCGGTCA TCAGAACAAC AGCTCAAACA AGAACTCAAC 
CATCTGCTGG AGCTCAAACT CACACTGGAG AAACAAAACC AGGAGCTGTC CAAGGAACGA 
GAAGAATCTG AAAAACAGCT GAAGGAGATG AAGGACCAGC TGGAGGCTGA GCAGTACTTC 
ACGAAACTGT ACAAGACTCA GATTCGAGAG CTGAAAGAGG AAAGCGATGA AAAGGTTAAA 
CTCTACAAAG ATGCTCAGCA GAGAATAGAG GACCTGCAGG AAGAGAGGGA CTCGTTAGCG 
TCTCAGTTGG AGGTGAGCCT GACGAAGGCT GACTCTGAAC AGTTGGCTCG CATCACCGTT 
GAAGAGCAAT ACTCGGACCT GGAGAAAGAG AAGATCATGA AGGAACTGGA GATAAAGGAC 
ATGATCGCCA GACACCGGCA GGATCTTGCA GAAAAGGACG GTACCATCAA CTCGCTGGAG 
GAGTCAAACC GTACGCTGAC TGTGGATGTT GCCAACCTGG CCAGTGAGAA AGAGGAGCTC 
AACAATAAAC TGAAGCACAT ACAGCAAAAG CTGGAGAAGA TCAAAGAAGA AGAGAAACAG 
ATGAAATCTC TCACAGTGTC GTATGAGAAG CAGATACAGG TGGAGAAAAC ACTCAAGATT 
CAGGCTATAA ACAAGCTGGC GGAGGTCATG AAGAAGACGG ACGGCAGGCC ACACCAAATC 
AACAACACAG ACATCCGCAG GAAGGAGAAG GAGATCAGAC AACTCCAGCT GCAGCTCCGC 
ACGGAGAAAG AAAAGCTTAA CCACTCCATC ATCAAATACC AGAGAGAAAT CAACGACATG 
CAGGCTATGA TAGCAGAGGA GTCTCAGGTG CGTCTGGAGA TGCAGATGTC TCTGGACAGT 
AAAGACAGTG ATATTGAGCG TCTGCGCAGT CAACTCACGT CTCTGAGCAT TCACTCTCTG 
GACACCACCA GCATCAGCAG CATCGGCAAT GACCTGGATT CTGACGAGGC ATATCCAGTG 
CGAATCACAC ACTCACACAC CTCGGAGTCT ATGTCCTTCA CATACCAGCG AAGCTCCAAG 
TCGGTGTGTA TTGATACCCG GCCCTGTCGA ATCGCTCCTC GCTTCTCCTC TGACTCTGAT 
GAAGAGGAGG ATGAGGAAGA TGAGTGGAGA CAAACACCTG CCCTTACATA TGAGACGCCT 
CCTGACCCTG AGGCTGCAGC CGCAGAGTCC GGTCTTGAAG GATGGCTCTC AATGCCTGCC 
AAAAACACCA AACGGTTTGG ATGGGATAAG AAGTATGTGG TTGTGAGCAG CAAGAAAATC 
CTGTTTTATG ACTCTGAACA AGACCGAGAG CAGTCCAGTC CATTCATGAT CCTGGACATT 
GACAAACTCT TCCACGTGAG ACCCGTCACT CAGACAGATG TTTACCGCGC AGATCCCAGA 
GAAATCCCTC GCATCTTCCA GATACTATAT GCGAATGAAG GAGAGAGCAA GAAAGAGGAG 
CTGCCCGTGG AGTCGTTATC TGTGACTGAG AGGTCACTGT GTATCCCACA CAAGGGTCAC 
GAGTTTGTTC TGACGCTTTA TCACTTCCCC TCGAGCTGCG AGGTCTGTCC ACGACCCTTA 
TGGCACGTCT TCAGACCTCC GCCGGCTCTC GAGTGCAGAC GCTGTAGAGT CAAGTGCCAT 
AAAGACCACA TCGATCGCAA AGAAGAGGTC CTCGCTCCAT GTAGAGTGAA TTACGACATG 
TCCACAGCGA AAGAGATGCT GCTGCTGGCA AACTCCACAG AGGAGCAGAA GAAGTGGGTC 
AGTCGGCTGC TCAAGAGGGT TCCGCGTAAA CCTTCCATCG CCCACTCTTC CAGCATAGCC 
ATATCCGCTG CGCCCTCAGA GGCAACACCC ACTACCCTTT CCCCTCAGCC GTCGCCTCGC 
CTCGCTCAGT CTTCACCCAG ACTTTCACAC CGAGCAGCCG TCAAAGTGCA CTCTACACGA 
CAGCAGCCCT CCAGCAAGAA CAGACTGGTT TGGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rock2b ( XM_017352435.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207924.1 Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa56542
Clone ID Related Accession (Same CDS sequence)	XM_017352435.1
Accession Version	XM_017352435.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4167bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	rho-associated, coiled-coil containing protein kinase 2b isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAAGCAGC TCAGCAAGTT TGAGATGGTC AAGCGCTCTG ACTCCGCCTT TTTCTGGGAG 
GAGCGTGACA TCATGGCTTT CTCCCAGAGC CCTTGGATAG TTCAGCTGTG CTGTGCATTC 
CAAGATGAAA AGTACCTTTA TTTGGTGATG GAGTTCATGC CAGGTGGAGA TCTGGTGACT 
CTGACCAGCA ACTATGACAT CCCAGAGGAA TGGGCTCAAT TCTATACGGC AGAAGTGGTG 
CTGGCACTCG ACGCCATCCA TTCTCTGGGC TTCATCCACA GAGACATCAA ACCAGACAAC 
ATGCTGCTGG ACCGCAATGG ACACCTCAAA CTCGCAGACT TTGGCACATG CATGAAGATG 
GACTCGACTG GTATGGTACG CTGTGACACT GCTGTTGGGA CACCTGATTA CATTTCTCCA 
GAGGTTTTAA TGTCTCAAGG AGGGACTGGT TATTACGGTC GCGAGTGTGA CTGGTGGTCT 
GTTGGTGTTT TTATTTATGA GCTTCTAGTG GGCGACACTC CATTTTATTC CGAGTCTCTG 
GTGGGCACAT ATGGAAAGAT CATGGATCAC AAGAACAGCT TAACTTTCCC AGATGATATT 
GAAATGTCAA AGAATGCCAA AGACCTCATC TGTGCCTTTC TGAGTAGCAG GGAGGTGAGG 
CTTGGCCGAA CTGGAGTGGA TGAAATAAAA TGTCACCCCT TCTTTAAGAA TGATCAGTGG 
ACCTTCGACA CCATACGAGA CACCATGGCT CCAGTGGTGC CAGAGCTGAG TAGCGATATT 
GACACCAGTA ACTTTGATGA TGACATTAAG GATGATCCGA TAGGAACAGA GACGTTCCCT 
CCGCCCCGTG CCTTCGCAGG GAACCAGCTA CCATTTGTGG GTTTCACCTA TTTCAGGGAG 
GACCAATTGT TAACACAACA AAATAAGTGT TCAGAGGAAG ATATCATTGA AGATCATGGC 
TTAAACCACA CAGAGTCTAA CGACCTGGAG AAGAGGCTGA AAAAGTTGGA GGAAAAATTC 
AAACATGAGA TGCAAGCAAA GGAAGAACTG GAAAATAAAT GCCGAATAGC AAATCAGCGG 
CTGGAGAAAT TGTCCAAAGA TCTTGAAGAA GAAGTGAATG CGAGACAGGA GGCGGAAGAC 
AACCTGAGAT CCCTGGAGAA AGAGAAAGTT TTACTGAAAC ACCAGCGTAC ACAGAGCGTA 
AGAAAAGCTG GTCTAGAGAC CGACAGAAAA CGGCTGCTGG AGAATGAAGT AAGCAGCCTG 
AAAGAGCAGC TTGCCGAACT GAAGAAAAAG AACCAGATCT CTCATCTGTC TGCTGAGAAG 
AACATTCACC TGGAAAGACA GCTGGAGGAG GTGAGTGCCA AGCTTCAGGC CGAGCTGGAG 
GAGTCGGAGA GGCTGAAGAA GGCCCAGATC GAGGCCTTTA GACAGTCCCA GCAGCTGGAG 
CTTTCCCTCA GGGAGCTGCA GGAGCGTCTG GCCCAGCTGG AGAACAGCAG GCTGGTTCTG 
GAGCAGGACA AACTCAGCCT GCAGACCTCG CTAGAGCTGG AGAAACGAGA GAGAAACGCC 
GGCTCAGAGA CCATCACTGA CCTGCAGGGT CGTATCTATG GACTGGAGGG TGAGTTGAAG 
CATATAAAGA GTTCTCTATC AAAAGCTGAA GTTGAGAAAA GACAATTACA AGAGGATCTT 
ACAACCCTGG AAAAGGAGAA GAATAAGCAG GAGATCGACC TCTCCTTCAA ACTCAAAGCA 
GTTCAGCAGA GTCTGGAGCA GGAGGAGGCT GAACATAAAA CCACTAAAGC CAGATTGGCC 
GACAACAACA AAATCAACCA ATCCATAGAG GCAAAGTCTG AGACCCTGAA AGACATGGAA 
CATAAGCTGT TGGAAGAACG ATCTGCGAAG CAACAACTGG AAAACAGACT GATGCAGCTG 
GAGAAGGAAA ACTCTGTATT AGACTGTGAT TACAAACAAG CCAAACATGA GCTTCAGGAG 
CTCCGCTCGC TCAAAGAAAA TCTCACAGAG CAGGTGGAGG TGCTGAATGT GCGGGTGCAG 
CAGGAGACAC AGAGGAAAAC TCTCTGTCAA GGTGATCTCA AAGTTCAGAG GCAGGAGATC 
AACAGCCTGC GGTCATCAGA ACAACAGCTC AAACAAGAAC TCAACCATCT GCTGGAGCTC 
AAACTCACAC TGGAGAAACA AAACCAGGAG CTGTCCAAGG AACGAGAAGA ATCTGAAAAA 
CAGCTGAAGG AGATGAAGGA CCAGCTGGAG GCTGAGCAGT ACTTCACGAA ACTGTACAAG 
ACTCAGATTC GAGAGCTGAA AGAGGAAAGC GATGAAAAGG TTAAACTCTA CAAAGATGCT 
CAGCAGAGAA TAGAGGACCT GCAGGAAGAG AGGGACTCGT TAGCGTCTCA GTTGGAGGTG 
AGCCTGACGA AGGCTGACTC TGAACAGTTG GCTCGCATCA CCGTTGAAGA GCAATACTCG 
GACCTGGAGA AAGAGAAGAT CATGAAGGAA CTGGAGATAA AGGACATGAT CGCCAGACAC 
CGGCAGGATC TTGCAGAAAA GGACGGTACC ATCAACTCGC TGGAGGAGTC AAACCGTACG 
CTGACTGTGG ATGTTGCCAA CCTGGCCAGT GAGAAAGAGG AGCTCAACAA TAAACTGAAG 
CACATACAGC AAAAGCTGGA GAAGATCAAA GAAGAAGAGA AACAGATGAA ATCTCTCACA 
GTGTCGTATG AGAAGCAGAT ACAGGTGGAG AAAACACTCA AGATTCAGGC TATAAACAAG 
CTGGCGGAGG TCATGAAGAA GACGGACGGC AGGCCACACC AAATCAACAA CACAGACATC 
CGCAGGAAGG AGAAGGAGAT CAGACAACTC CAGCTGCAGC TCCGCACGGA GAAAGAAAAG 
CTTAACCACT CCATCATCAA ATACCAGAGA GAAATCAACG ACATGCAGGC TATGATAGCA 
GAGGAGTCTC AGGTGCGTCT GGAGATGCAG ATGTCTCTGG ACAGTAAAGA CAGTGATATT 
GAGCGTCTGC GCAGTCAACT CACGTCTCTG AGCATTCACT CTCTGGACAC CACCAGCATC 
AGCAGCATCG GCAATGACCT GGATTCTGAC GAGGCATATC CAGTGCGAAT CACACACTCA 
CACACCTCGG AGTCTATGTC CTTCACATAC CAGCGAAGCT CCAAGTCGGT GTGTATTGAT 
ACCCGGCCCT GTCGAATCGC TCCTCGCTTC TCCTCTGACT CTGATGAAGA GGAGGATGAG 
GAAGATGAGT GGAGACAAAC ACCTGCCCTT ACATATGAGA CGCCTCCTGA CCCTGAGGCT 
GCAGCCGCAG AGTCCGGTCT TGAAGGATGG CTCTCAATGC CTGCCAAAAA CACCAAACGG 
TTTGGATGGG ATAAGAAGTA TGTGGTTGTG AGCAGCAAGA AAATCCTGTT TTATGACTCT 
GAACAAGACC GAGAGCAGTC CAGTCCATTC ATGATCCTGG ACATTGACAA ACTCTTCCAC 
GTGAGACCCG TCACTCAGAC AGATGTTTAC CGCGCAGATC CCAGAGAAAT CCCTCGCATC 
TTCCAGATAC TATATGCGAA TGAAGGAGAG AGCAAGAAAG AGGAGCTGCC CGTGGAGTCG 
TTATCTGTGA CTGAGAGGTC ACTGTGTATC CCACACAAGG GTCACGAGTT TGTTCTGACG 
CTTTATCACT TCCCCTCGAG CTGCGAGGTC TGTCCACGAC CCTTATGGCA CGTCTTCAGA 
CCTCCGCCGG CTCTCGAGTG CAGACGCTGT AGAGTCAAGT GCCATAAAGA CCACATCGAT 
CGCAAAGAAG AGGTCCTCGC TCCATGTAGA GTGAATTACG ACATGTCCAC AGCGAAAGAG 
ATGCTGCTGC TGGCAAACTC CACAGAGGAG CAGAAGAAGT GGGTCAGTCG GCTGCTCAAG 
AGGGTTCCGC GTAAACCTTC CATCGCCCAC TCTTCCAGCA TAGCCATATC CGCTGCGCCC 
TCAGAGGCAA CACCCACTAC CCTTTCCCCT CAGCCGTCGC CTCGCCTCGC TCAGTCTTCA 
CCCAGACTTT CACACCGAGC AGCCGTCAAA GTGCACTCTA CACGACAGCA GCCCTCCAGC 
AAGAACAGAT TGCTTGAAAT GGGCCTGAAG GACTGGGGTT GGGCTCTTGA GGTTGAATTG 
GATGATGACG ATGTTTTAGA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207924.1
CDS	236..4402
Translation	MKQLSKFEMVKRSDSAFFWEERDIMAFSQSPWIVQLCCAFQDEKYLYLVMEFMPGGDLVTLTSNYDIPEEWAQFYTAEVVLALDAIHSLGFIHRDIKPDNMLLDRNGHLKLADFGTCMKMDSTGMVRCDTAVGTPDYISPEVLMSQGGTGYYGRECDWWSVGVFIYELLVGDTPFYSESLVGTYGKIMDHKNSLTFPDDIEMSKNAKDLICAFLSSREVRLGRTGVDEIKCHPFFKNDQWTFDTIRDTMAPVVPELSSDIDTSNFDDDIKDDPIGTETFPPPRAFAGNQLPFVGFTYFREDQLLTQQNKCSEEDIIEDHGLNHTESNDLEKRLKKLEEKFKHEMQAKEELENKCRIANQRLEKLSKDLEEEVNARQEAEDNLRSLEKEKVLLKHQRTQSVRKAGLETDRKRLLENEVSSLKEQLAELKKKNQISHLSAEKNIHLERQLEEVSAKLQAELEESERLKKAQIEAFRQSQQLELSLRELQERLAQLENSRLVLEQDKLSLQTSLELEKRERNAGSETITDLQGRIYGLEGELKHIKSSLSKAEVEKRQLQEDLTTLEKEKNKQEIDLSFKLKAVQQSLEQEEAEHKTTKARLADNNKINQSIEAKSETLKDMEHKLLEERSAKQQLENRLMQLEKENSVLDCDYKQAKHELQELRSLKENLTEQVEVLNVRVQQETQRKTLCQGDLKVQRQEINSLRSSEQQLKQELNHLLELKLTLEKQNQELSKEREESEKQLKEMKDQLEAEQYFTKLYKTQIRELKEESDEKVKLYKDAQQRIEDLQEERDSLASQLEVSLTKADSEQLARITVEEQYSDLEKEKIMKELEIKDMIARHRQDLAEKDGTINSLEESNRTLTVDVANLASEKEELNNKLKHIQQKLEKIKEEEKQMKSLTVSYEKQIQVEKTLKIQAINKLAEVMKKTDGRPHQINNTDIRRKEKEIRQLQLQLRTEKEKLNHSIIKYQREINDMQAMIAEESQVRLEMQMSLDSKDSDIERLRSQLTSLSIHSLDTTSISSIGNDLDSDEAYPVRITHSHTSESMSFTYQRSSKSVCIDTRPCRIAPRFSSDSDEEEDEEDEWRQTPALTYETPPDPEAAAAESGLEGWLSMPAKNTKRFGWDKKYVVVSSKKILFYDSEQDREQSSPFMILDIDKLFHVRPVTQTDVYRADPREIPRIFQILYANEGESKKEELPVESLSVTERSLCIPHKGHEFVLTLYHFPSSCEVCPRPLWHVFRPPPALECRRCRVKCHKDHIDRKEEVLAPCRVNYDMSTAKEMLLLANSTEEQKKWVSRLLKRVPRKPSIAHSSSIAISAAPSEATPTTLSPQPSPRLAQSSPRLSHRAAVKVHSTRQQPSSKNRLLEMGLKDWGWALEVELDDDDVLDF

Target ORF information:

RefSeq Version	XM_017352435.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352435.1

ORF Insert Sequence:

ATGAAGCAGC TCAGCAAGTT TGAGATGGTC AAGCGCTCTG ACTCCGCCTT TTTCTGGGAG 
GAGCGTGACA TCATGGCTTT CTCCCAGAGC CCTTGGATAG TTCAGCTGTG CTGTGCATTC 
CAAGATGAAA AGTACCTTTA TTTGGTGATG GAGTTCATGC CAGGTGGAGA TCTGGTGACT 
CTGACCAGCA ACTATGACAT CCCAGAGGAA TGGGCTCAAT TCTATACGGC AGAAGTGGTG 
CTGGCACTCG ACGCCATCCA TTCTCTGGGC TTCATCCACA GAGACATCAA ACCAGACAAC 
ATGCTGCTGG ACCGCAATGG ACACCTCAAA CTCGCAGACT TTGGCACATG CATGAAGATG 
GACTCGACTG GTATGGTACG CTGTGACACT GCTGTTGGGA CACCTGATTA CATTTCTCCA 
GAGGTTTTAA TGTCTCAAGG AGGGACTGGT TATTACGGTC GCGAGTGTGA CTGGTGGTCT 
GTTGGTGTTT TTATTTATGA GCTTCTAGTG GGCGACACTC CATTTTATTC CGAGTCTCTG 
GTGGGCACAT ATGGAAAGAT CATGGATCAC AAGAACAGCT TAACTTTCCC AGATGATATT 
GAAATGTCAA AGAATGCCAA AGACCTCATC TGTGCCTTTC TGAGTAGCAG GGAGGTGAGG 
CTTGGCCGAA CTGGAGTGGA TGAAATAAAA TGTCACCCCT TCTTTAAGAA TGATCAGTGG 
ACCTTCGACA CCATACGAGA CACCATGGCT CCAGTGGTGC CAGAGCTGAG TAGCGATATT 
GACACCAGTA ACTTTGATGA TGACATTAAG GATGATCCGA TAGGAACAGA GACGTTCCCT 
CCGCCCCGTG CCTTCGCAGG GAACCAGCTA CCATTTGTGG GTTTCACCTA TTTCAGGGAG 
GACCAATTGT TAACACAACA AAATAAGTGT TCAGAGGAAG ATATCATTGA AGATCATGGC 
TTAAACCACA CAGAGTCTAA CGACCTGGAG AAGAGGCTGA AAAAGTTGGA GGAAAAATTC 
AAACATGAGA TGCAAGCAAA GGAAGAACTG GAAAATAAAT GCCGAATAGC AAATCAGCGG 
CTGGAGAAAT TGTCCAAAGA TCTTGAAGAA GAAGTGAATG CGAGACAGGA GGCGGAAGAC 
AACCTGAGAT CCCTGGAGAA AGAGAAAGTT TTACTGAAAC ACCAGCGTAC ACAGAGCGTA 
AGAAAAGCTG GTCTAGAGAC CGACAGAAAA CGGCTGCTGG AGAATGAAGT AAGCAGCCTG 
AAAGAGCAGC TTGCCGAACT GAAGAAAAAG AACCAGATCT CTCATCTGTC TGCTGAGAAG 
AACATTCACC TGGAAAGACA GCTGGAGGAG GTGAGTGCCA AGCTTCAGGC CGAGCTGGAG 
GAGTCGGAGA GGCTGAAGAA GGCCCAGATC GAGGCCTTTA GACAGTCCCA GCAGCTGGAG 
CTTTCCCTCA GGGAGCTGCA GGAGCGTCTG GCCCAGCTGG AGAACAGCAG GCTGGTTCTG 
GAGCAGGACA AACTCAGCCT GCAGACCTCG CTAGAGCTGG AGAAACGAGA GAGAAACGCC 
GGCTCAGAGA CCATCACTGA CCTGCAGGGT CGTATCTATG GACTGGAGGG TGAGTTGAAG 
CATATAAAGA GTTCTCTATC AAAAGCTGAA GTTGAGAAAA GACAATTACA AGAGGATCTT 
ACAACCCTGG AAAAGGAGAA GAATAAGCAG GAGATCGACC TCTCCTTCAA ACTCAAAGCA 
GTTCAGCAGA GTCTGGAGCA GGAGGAGGCT GAACATAAAA CCACTAAAGC CAGATTGGCC 
GACAACAACA AAATCAACCA ATCCATAGAG GCAAAGTCTG AGACCCTGAA AGACATGGAA 
CATAAGCTGT TGGAAGAACG ATCTGCGAAG CAACAACTGG AAAACAGACT GATGCAGCTG 
GAGAAGGAAA ACTCTGTATT AGACTGTGAT TACAAACAAG CCAAACATGA GCTTCAGGAG 
CTCCGCTCGC TCAAAGAAAA TCTCACAGAG CAGGTGGAGG TGCTGAATGT GCGGGTGCAG 
CAGGAGACAC AGAGGAAAAC TCTCTGTCAA GGTGATCTCA AAGTTCAGAG GCAGGAGATC 
AACAGCCTGC GGTCATCAGA ACAACAGCTC AAACAAGAAC TCAACCATCT GCTGGAGCTC 
AAACTCACAC TGGAGAAACA AAACCAGGAG CTGTCCAAGG AACGAGAAGA ATCTGAAAAA 
CAGCTGAAGG AGATGAAGGA CCAGCTGGAG GCTGAGCAGT ACTTCACGAA ACTGTACAAG 
ACTCAGATTC GAGAGCTGAA AGAGGAAAGC GATGAAAAGG TTAAACTCTA CAAAGATGCT 
CAGCAGAGAA TAGAGGACCT GCAGGAAGAG AGGGACTCGT TAGCGTCTCA GTTGGAGGTG 
AGCCTGACGA AGGCTGACTC TGAACAGTTG GCTCGCATCA CCGTTGAAGA GCAATACTCG 
GACCTGGAGA AAGAGAAGAT CATGAAGGAA CTGGAGATAA AGGACATGAT CGCCAGACAC 
CGGCAGGATC TTGCAGAAAA GGACGGTACC ATCAACTCGC TGGAGGAGTC AAACCGTACG 
CTGACTGTGG ATGTTGCCAA CCTGGCCAGT GAGAAAGAGG AGCTCAACAA TAAACTGAAG 
CACATACAGC AAAAGCTGGA GAAGATCAAA GAAGAAGAGA AACAGATGAA ATCTCTCACA 
GTGTCGTATG AGAAGCAGAT ACAGGTGGAG AAAACACTCA AGATTCAGGC TATAAACAAG 
CTGGCGGAGG TCATGAAGAA GACGGACGGC AGGCCACACC AAATCAACAA CACAGACATC 
CGCAGGAAGG AGAAGGAGAT CAGACAACTC CAGCTGCAGC TCCGCACGGA GAAAGAAAAG 
CTTAACCACT CCATCATCAA ATACCAGAGA GAAATCAACG ACATGCAGGC TATGATAGCA 
GAGGAGTCTC AGGTGCGTCT GGAGATGCAG ATGTCTCTGG ACAGTAAAGA CAGTGATATT 
GAGCGTCTGC GCAGTCAACT CACGTCTCTG AGCATTCACT CTCTGGACAC CACCAGCATC 
AGCAGCATCG GCAATGACCT GGATTCTGAC GAGGCATATC CAGTGCGAAT CACACACTCA 
CACACCTCGG AGTCTATGTC CTTCACATAC CAGCGAAGCT CCAAGTCGGT GTGTATTGAT 
ACCCGGCCCT GTCGAATCGC TCCTCGCTTC TCCTCTGACT CTGATGAAGA GGAGGATGAG 
GAAGATGAGT GGAGACAAAC ACCTGCCCTT ACATATGAGA CGCCTCCTGA CCCTGAGGCT 
GCAGCCGCAG AGTCCGGTCT TGAAGGATGG CTCTCAATGC CTGCCAAAAA CACCAAACGG 
TTTGGATGGG ATAAGAAGTA TGTGGTTGTG AGCAGCAAGA AAATCCTGTT TTATGACTCT 
GAACAAGACC GAGAGCAGTC CAGTCCATTC ATGATCCTGG ACATTGACAA ACTCTTCCAC 
GTGAGACCCG TCACTCAGAC AGATGTTTAC CGCGCAGATC CCAGAGAAAT CCCTCGCATC 
TTCCAGATAC TATATGCGAA TGAAGGAGAG AGCAAGAAAG AGGAGCTGCC CGTGGAGTCG 
TTATCTGTGA CTGAGAGGTC ACTGTGTATC CCACACAAGG GTCACGAGTT TGTTCTGACG 
CTTTATCACT TCCCCTCGAG CTGCGAGGTC TGTCCACGAC CCTTATGGCA CGTCTTCAGA 
CCTCCGCCGG CTCTCGAGTG CAGACGCTGT AGAGTCAAGT GCCATAAAGA CCACATCGAT 
CGCAAAGAAG AGGTCCTCGC TCCATGTAGA GTGAATTACG ACATGTCCAC AGCGAAAGAG 
ATGCTGCTGC TGGCAAACTC CACAGAGGAG CAGAAGAAGT GGGTCAGTCG GCTGCTCAAG 
AGGGTTCCGC GTAAACCTTC CATCGCCCAC TCTTCCAGCA TAGCCATATC CGCTGCGCCC 
TCAGAGGCAA CACCCACTAC CCTTTCCCCT CAGCCGTCGC CTCGCCTCGC TCAGTCTTCA 
CCCAGACTTT CACACCGAGC AGCCGTCAAA GTGCACTCTA CACGACAGCA GCCCTCCAGC 
AAGAACAGAT TGCTTGAAAT GGGCCTGAAG GACTGGGGTT GGGCTCTTGA GGTTGAATTG 
GATGATGACG ATGTTTTAGA CTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rock2b ( XM_017352437.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207926.1 Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X10, mRNA.

Price & Availability↑

CloneID	ODa24920
Clone ID Related Accession (Same CDS sequence)	XM_017352437.2 , XM_017352437.1
Accession Version	XM_017352437.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho-associated, coiled-coil containing protein kinase 2b isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352437.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGACCG GAGCGGGGAA GAGAATAGAG AGCCGGGTGA GAAAACTAGA GACGCTGATC 
AAGAACCAGA AATCACCGTT GAACTTGGAG AGTTTGCTGG ATTCAGTAAC TGCCCTTGCC 
CTTGATCTGA ACCATCCAGC ACTGAGAAAG AACAAAAACA TAGATGCCTT CTTAAGCAGA 
TATGAAAAAG CCGTGTGCAA CCTCAAGGAA CTACAGGTGA AGCTGGATGA TTTTGATCGA 
GTCAAGCTGA TCGGTCGTGG AGCGTTTGGC GCAGTGCAGC TGGTACGGCA CAAAGTATCT 
CAACAAGTTT ATGCCATGAA GCAGCTCAGC AAGTTTGAGA TGGTCAAGCG CTCTGACTCC 
GCCTTTTTCT GGGAGGAGCG TGACATCATG GCTTTCTCCC AGAGCCCTTG GATAGTTCAG 
CTGTGCTGTG CATTCCAAGA TGAAAAGTAC CTTTATTTGG TGATGGAGTT CATGCCAGGT 
GGAGATCTGG TGACTCTGAC CAGCAACTAT GACATCCCAG AGGAATGGGC TCAATTCTAT 
ACGGCAGAAG TGGTGCTGGC ACTCGACGCC ATCCATTCTC TGGGCTTCAT CCACAGAGAC 
ATCAAACCAG ACAACATGCT GCTGGACCGC AATGGACACC TCAAACTCGC AGACTTTGGC 
ACATGCATGA AGATGGACTC GACTGGTATG GTACGCTGTG ACACTGCTGT TGGGACACCT 
GATTACATTT CTCCAGAGGT TTTAATGTCT CAAGGAGGGA CTGGTTATTA CGGTCGCGAG 
TGTGACTGGT GGTCTGTTGG TGTTTTTATT TATGAGCTTC TAGTGGGCGA CACTCCATTT 
TATTCCGAGT CTCTGGTGGG CACATATGGA AAGATCATGG ATCACAAGAA CAGCTTAACT 
TTCCCAGATG ATATTGAAAT GTCAAAGAAT GCCAAAGACC TCATCTGTGC CTTTCTGAGT 
AGCAGGGAGG TGAGGCTTGG CCGAACTGGA GTGGATGAAA TAAAATGTCA CCCCTTCTTT 
AAGAATGATC AGTGGACCTT CGACACCATA CGAGACACCA TGGCTCCAGT GGTGCCAGAG 
CTGAGTAGCG ATATTGACAC CAGTAACTTT GATGATGACA TTAAGGATGA TCCGATAGGA 
ACAGAGACGT TCCCTCCGCC CCGTGCCTTC GCAGGGAACC AGCTACCATT TGTGGGTTTC 
ACCTATTTCA GGGAGGACCA ATTGTTAACA CAACAAAATA AGTGTTCAGA GGAAGATATC 
ATTGAAGATC ATGGCTTAAA CCACACAGAG TCTAACGACC TGGAGAAGAG GCTGAAAAAG 
TTGGAGGAAA AATTCAAACA TGAGATGCAA GCAAAGGAAG AACTGGAAAA TAAATGCCGA 
ATAGCAAATC AGCGGCTGGA GAAATTGTCC AAAGATCTTG AAGAAGAAGT GAATGCGAGA 
CAGGAGGCGG AAGACAACCT GAGATCCCTG GAGAAAGAGA AAGTTTTACT GAAACACCAG 
CGTACACAGA GCGTAAGAAA AGCTGGTCTA GAGACCGACA GAAAACGGCT GCTGGAGAAT 
GAAGTAAGCA GCCTGAAAGA GCAGCTTGCC GAACTGAAGA AAAAGAACCA GATCTCTCAT 
CTGTCTGCTG AGAAGAACAT TCACCTGGAA AGACAGCTGG AGGAGGTGAG TGCCAAGCTT 
CAGGCCGAGC TGGAGGAGTC GGAGAGGCTG AAGAAGGCCC AGATCGAGGC CTTTAGACAG 
TCCCAGCAGC TGGAGCTTTC CCTCAGGGAG CTGCAGGAGC GTCTGGCCCA GCTGGAGAAC 
AGCAGGCTGG TTCTGGAGCA GGACAAACTC AGCCTGCAGA CCTCGCTAGA GCTGGAGAAA 
CGAGAGAGAA ACGCCGGCTC AGAGACCATC ACTGACCTGC AGGGTCGTAT CTATGGACTG 
GAGGGTGAGT TGAAGCATAT AAAGAGTTCT CTATCAAAAG CTGAAGTTGA GAAAAGACAA 
TTACAAGAGG ATCTTACAAC CCTGGAAAAG GAGAAGAATA AGCAGGAGAT CGACCTCTCC 
TTCAAACTCA AAGCAGTTCA GCAGAGTCTG GAGCAGGAGG AGGCTGAACA TAAAACCACT 
AAAGCCAGAT TGGCCGACAA CAACAAAATC AACCAATCCA TAGAGGCAAA GTCTGAGACC 
CTGAAAGACA TGGAACATAA GCTGTTGGAA GAACGATCTG CGAAGCAACA ACTGGAAAAC 
AGACTGATGC AGCTGGAGAA GGAAAACTCT GTATTAGACT GTGATTACAA ACAAGCCAAA 
CATGAGCTTC AGGAGCTCCG CTCGCTCAAA GAAAATCTCA CAGAGCAGGT GGAGGTGCTG 
AATGTGCGGG TGCAGCAGGA GACACAGAGG AAAACTCTCT GTCAAGGTGA TCTCAAAGTT 
CAGAGGCAGG AGATCAACAG CCTGCGGTCA TCAGAACAAC AGCTCAAACA AGAACTCAAC 
CATCTGCTGG AGCTCAAACT CACACTGGAG AAACAAAACC AGGAGCTGTC CAAGGAACGA 
GAAGAATCTG AAAAACAGCT GAAGGAGATG AAGGACCAGC TGGAGGCTGA GCAGTACTTC 
ACGAAACTGT ACAAGACTCA GATTCGAGAG CTGAAAGAGG AAAGCGATGA AAAGGTTAAA 
CTCTACAAAG ATGCTCAGCA GAGAATAGAG GACCTGCAGG AAGAGAGGGA CTCGTTAGCG 
TCTCAGTTGG AGGTGAGCCT GACGAAGGCT GACTCTGAAC AGTTGGCTCG CATCACCGTT 
GAAGAGCAAT ACTCGGACCT GGAGAAAGAG AAGATCATGA AGGAACTGGA GATAAAGGAC 
ATGATCGCCA GACACCGGCA GGATCTTGCA GAAAAGGACG GTACCATCAA CTCGCTGGAG 
GAGTCAAACC GTACGCTGAC TGTGGATGTT GCCAACCTGG CCAGTGAGAA AGAGGAGCTC 
AACAATAAAC TGAAGCACAT ACAGCAAAAG CTGGAGAAGA TCAAAGAAGA AGAGAAACAG 
ATGAAATCTC TCACAGTGTC GTATGAGAAG CAGATACAGG TGGAGAAAAC ACTCAAGATT 
CAGGCTATAA ACAAGCTGGC GGAGGTCATG AAGAAGACGG ACGGCAGGCC ACACCAAATC 
AACAACACAG ACATCCGCAG GAAGGAGAAG GAGATCAGAC AACTCCAGCT GCAGCTCCGC 
ACGGAGAAAG AAAAGCTTAA CCACTCCATC ATCAAATACC AGAGAGAAAT CAACGACATG 
CAGGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207926.1
CDS	477..3725
Translation	MSTGAGKRIESRVRKLETLIKNQKSPLNLESLLDSVTALALDLNHPALRKNKNIDAFLSRYEKAVCNLKELQVKLDDFDRVKLIGRGAFGAVQLVRHKVSQQVYAMKQLSKFEMVKRSDSAFFWEERDIMAFSQSPWIVQLCCAFQDEKYLYLVMEFMPGGDLVTLTSNYDIPEEWAQFYTAEVVLALDAIHSLGFIHRDIKPDNMLLDRNGHLKLADFGTCMKMDSTGMVRCDTAVGTPDYISPEVLMSQGGTGYYGRECDWWSVGVFIYELLVGDTPFYSESLVGTYGKIMDHKNSLTFPDDIEMSKNAKDLICAFLSSREVRLGRTGVDEIKCHPFFKNDQWTFDTIRDTMAPVVPELSSDIDTSNFDDDIKDDPIGTETFPPPRAFAGNQLPFVGFTYFREDQLLTQQNKCSEEDIIEDHGLNHTESNDLEKRLKKLEEKFKHEMQAKEELENKCRIANQRLEKLSKDLEEEVNARQEAEDNLRSLEKEKVLLKHQRTQSVRKAGLETDRKRLLENEVSSLKEQLAELKKKNQISHLSAEKNIHLERQLEEVSAKLQAELEESERLKKAQIEAFRQSQQLELSLRELQERLAQLENSRLVLEQDKLSLQTSLELEKRERNAGSETITDLQGRIYGLEGELKHIKSSLSKAEVEKRQLQEDLTTLEKEKNKQEIDLSFKLKAVQQSLEQEEAEHKTTKARLADNNKINQSIEAKSETLKDMEHKLLEERSAKQQLENRLMQLEKENSVLDCDYKQAKHELQELRSLKENLTEQVEVLNVRVQQETQRKTLCQGDLKVQRQEINSLRSSEQQLKQELNHLLELKLTLEKQNQELSKEREESEKQLKEMKDQLEAEQYFTKLYKTQIRELKEESDEKVKLYKDAQQRIEDLQEERDSLASQLEVSLTKADSEQLARITVEEQYSDLEKEKIMKELEIKDMIARHRQDLAEKDGTINSLEESNRTLTVDVANLASEKEELNNKLKHIQQKLEKIKEEEKQMKSLTVSYEKQIQVEKTLKIQAINKLAEVMKKTDGRPHQINNTDIRRKEKEIRQLQLQLRTEKEKLNHSIIKYQREINDMQA

Target ORF information:

RefSeq Version	XM_017352437.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352437.2

ORF Insert Sequence:

ATGTCGACCG GAGCGGGGAA GAGAATAGAG AGCCGGGTGA GAAAACTAGA GACGCTGATC 
AAGAACCAGA AATCACCGTT GAACTTGGAG AGTTTGCTGG ATTCAGTAAC TGCCCTTGCC 
CTTGATCTGA ACCATCCAGC ACTGAGAAAG AACAAAAACA TAGATGCCTT CTTAAGCAGA 
TATGAAAAAG CCGTGTGCAA CCTCAAGGAA CTACAGGTGA AGCTGGATGA TTTTGATCGA 
GTCAAGCTGA TCGGTCGTGG AGCGTTTGGC GCAGTGCAGC TGGTACGGCA CAAAGTATCT 
CAACAAGTTT ATGCCATGAA GCAGCTCAGC AAGTTTGAGA TGGTCAAGCG CTCTGACTCC 
GCCTTTTTCT GGGAGGAGCG TGACATCATG GCTTTCTCCC AGAGCCCTTG GATAGTTCAG 
CTGTGCTGTG CATTCCAAGA TGAAAAGTAC CTTTATTTGG TGATGGAGTT CATGCCAGGT 
GGAGATCTGG TGACTCTGAC CAGCAACTAT GACATCCCAG AGGAATGGGC TCAATTCTAT 
ACGGCAGAAG TGGTGCTGGC ACTCGACGCC ATCCATTCTC TGGGCTTCAT CCACAGAGAC 
ATCAAACCAG ACAACATGCT GCTGGACCGC AATGGACACC TCAAACTCGC AGACTTTGGC 
ACATGCATGA AGATGGACTC GACTGGTATG GTACGCTGTG ACACTGCTGT TGGGACACCT 
GATTACATTT CTCCAGAGGT TTTAATGTCT CAAGGAGGGA CTGGTTATTA CGGTCGCGAG 
TGTGACTGGT GGTCTGTTGG TGTTTTTATT TATGAGCTTC TAGTGGGCGA CACTCCATTT 
TATTCCGAGT CTCTGGTGGG CACATATGGA AAGATCATGG ATCACAAGAA CAGCTTAACT 
TTCCCAGATG ATATTGAAAT GTCAAAGAAT GCCAAAGACC TCATCTGTGC CTTTCTGAGT 
AGCAGGGAGG TGAGGCTTGG CCGAACTGGA GTGGATGAAA TAAAATGTCA CCCCTTCTTT 
AAGAATGATC AGTGGACCTT CGACACCATA CGAGACACCA TGGCTCCAGT GGTGCCAGAG 
CTGAGTAGCG ATATTGACAC CAGTAACTTT GATGATGACA TTAAGGATGA TCCGATAGGA 
ACAGAGACGT TCCCTCCGCC CCGTGCCTTC GCAGGGAACC AGCTACCATT TGTGGGTTTC 
ACCTATTTCA GGGAGGACCA ATTGTTAACA CAACAAAATA AGTGTTCAGA GGAAGATATC 
ATTGAAGATC ATGGCTTAAA CCACACAGAG TCTAACGACC TGGAGAAGAG GCTGAAAAAG 
TTGGAGGAAA AATTCAAACA TGAGATGCAA GCAAAGGAAG AACTGGAAAA TAAATGCCGA 
ATAGCAAATC AGCGGCTGGA GAAATTGTCC AAAGATCTTG AAGAAGAAGT GAATGCGAGA 
CAGGAGGCGG AAGACAACCT GAGATCCCTG GAGAAAGAGA AAGTTTTACT GAAACACCAG 
CGTACACAGA GCGTAAGAAA AGCTGGTCTA GAGACCGACA GAAAACGGCT GCTGGAGAAT 
GAAGTAAGCA GCCTGAAAGA GCAGCTTGCC GAACTGAAGA AAAAGAACCA GATCTCTCAT 
CTGTCTGCTG AGAAGAACAT TCACCTGGAA AGACAGCTGG AGGAGGTGAG TGCCAAGCTT 
CAGGCCGAGC TGGAGGAGTC GGAGAGGCTG AAGAAGGCCC AGATCGAGGC CTTTAGACAG 
TCCCAGCAGC TGGAGCTTTC CCTCAGGGAG CTGCAGGAGC GTCTGGCCCA GCTGGAGAAC 
AGCAGGCTGG TTCTGGAGCA GGACAAACTC AGCCTGCAGA CCTCGCTAGA GCTGGAGAAA 
CGAGAGAGAA ACGCCGGCTC AGAGACCATC ACTGACCTGC AGGGTCGTAT CTATGGACTG 
GAGGGTGAGT TGAAGCATAT AAAGAGTTCT CTATCAAAAG CTGAAGTTGA GAAAAGACAA 
TTACAAGAGG ATCTTACAAC CCTGGAAAAG GAGAAGAATA AGCAGGAGAT CGACCTCTCC 
TTCAAACTCA AAGCAGTTCA GCAGAGTCTG GAGCAGGAGG AGGCTGAACA TAAAACCACT 
AAAGCCAGAT TGGCCGACAA CAACAAAATC AACCAATCCA TAGAGGCAAA GTCTGAGACC 
CTGAAAGACA TGGAACATAA GCTGTTGGAA GAACGATCTG CGAAGCAACA ACTGGAAAAC 
AGACTGATGC AGCTGGAGAA GGAAAACTCT GTATTAGACT GTGATTACAA ACAAGCCAAA 
CATGAGCTTC AGGAGCTCCG CTCGCTCAAA GAAAATCTCA CAGAGCAGGT GGAGGTGCTG 
AATGTGCGGG TGCAGCAGGA GACACAGAGG AAAACTCTCT GTCAAGGTGA TCTCAAAGTT 
CAGAGGCAGG AGATCAACAG CCTGCGGTCA TCAGAACAAC AGCTCAAACA AGAACTCAAC 
CATCTGCTGG AGCTCAAACT CACACTGGAG AAACAAAACC AGGAGCTGTC CAAGGAACGA 
GAAGAATCTG AAAAACAGCT GAAGGAGATG AAGGACCAGC TGGAGGCTGA GCAGTACTTC 
ACGAAACTGT ACAAGACTCA GATTCGAGAG CTGAAAGAGG AAAGCGATGA AAAGGTTAAA 
CTCTACAAAG ATGCTCAGCA GAGAATAGAG GACCTGCAGG AAGAGAGGGA CTCGTTAGCG 
TCTCAGTTGG AGGTGAGCCT GACGAAGGCT GACTCTGAAC AGTTGGCTCG CATCACCGTT 
GAAGAGCAAT ACTCGGACCT GGAGAAAGAG AAGATCATGA AGGAACTGGA GATAAAGGAC 
ATGATCGCCA GACACCGGCA GGATCTTGCA GAAAAGGACG GTACCATCAA CTCGCTGGAG 
GAGTCAAACC GTACGCTGAC TGTGGATGTT GCCAACCTGG CCAGTGAGAA AGAGGAGCTC 
AACAATAAAC TGAAGCACAT ACAGCAAAAG CTGGAGAAGA TCAAAGAAGA AGAGAAACAG 
ATGAAATCTC TCACAGTGTC GTATGAGAAG CAGATACAGG TGGAGAAAAC ACTCAAGATT 
CAGGCTATAA ACAAGCTGGC GGAGGTCATG AAGAAGACGG ACGGCAGGCC ACACCAAATC 
AACAACACAG ACATCCGCAG GAAGGAGAAG GAGATCAGAC AACTCCAGCT GCAGCTCCGC 
ACGGAGAAAG AAAAGCTTAA CCACTCCATC ATCAAATACC AGAGAGAAAT CAACGACATG 
CAGGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rock2b ( XM_017352437.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207926.1 Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X7, mRNA.

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CloneID	ODa24920
Clone ID Related Accession (Same CDS sequence)	XM_017352437.2 , XM_017352437.1
Accession Version	XM_017352437.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	rho-associated, coiled-coil containing protein kinase 2b isoform X7
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGACCG GAGCGGGGAA GAGAATAGAG AGCCGGGTGA GAAAACTAGA GACGCTGATC 
AAGAACCAGA AATCACCGTT GAACTTGGAG AGTTTGCTGG ATTCAGTAAC TGCCCTTGCC 
CTTGATCTGA ACCATCCAGC ACTGAGAAAG AACAAAAACA TAGATGCCTT CTTAAGCAGA 
TATGAAAAAG CCGTGTGCAA CCTCAAGGAA CTACAGGTGA AGCTGGATGA TTTTGATCGA 
GTCAAGCTGA TCGGTCGTGG AGCGTTTGGC GCAGTGCAGC TGGTACGGCA CAAAGTATCT 
CAACAAGTTT ATGCCATGAA GCAGCTCAGC AAGTTTGAGA TGGTCAAGCG CTCTGACTCC 
GCCTTTTTCT GGGAGGAGCG TGACATCATG GCTTTCTCCC AGAGCCCTTG GATAGTTCAG 
CTGTGCTGTG CATTCCAAGA TGAAAAGTAC CTTTATTTGG TGATGGAGTT CATGCCAGGT 
GGAGATCTGG TGACTCTGAC CAGCAACTAT GACATCCCAG AGGAATGGGC TCAATTCTAT 
ACGGCAGAAG TGGTGCTGGC ACTCGACGCC ATCCATTCTC TGGGCTTCAT CCACAGAGAC 
ATCAAACCAG ACAACATGCT GCTGGACCGC AATGGACACC TCAAACTCGC AGACTTTGGC 
ACATGCATGA AGATGGACTC GACTGGTATG GTACGCTGTG ACACTGCTGT TGGGACACCT 
GATTACATTT CTCCAGAGGT TTTAATGTCT CAAGGAGGGA CTGGTTATTA CGGTCGCGAG 
TGTGACTGGT GGTCTGTTGG TGTTTTTATT TATGAGCTTC TAGTGGGCGA CACTCCATTT 
TATTCCGAGT CTCTGGTGGG CACATATGGA AAGATCATGG ATCACAAGAA CAGCTTAACT 
TTCCCAGATG ATATTGAAAT GTCAAAGAAT GCCAAAGACC TCATCTGTGC CTTTCTGAGT 
AGCAGGGAGG TGAGGCTTGG CCGAACTGGA GTGGATGAAA TAAAATGTCA CCCCTTCTTT 
AAGAATGATC AGTGGACCTT CGACACCATA CGAGACACCA TGGCTCCAGT GGTGCCAGAG 
CTGAGTAGCG ATATTGACAC CAGTAACTTT GATGATGACA TTAAGGATGA TCCGATAGGA 
ACAGAGACGT TCCCTCCGCC CCGTGCCTTC GCAGGGAACC AGCTACCATT TGTGGGTTTC 
ACCTATTTCA GGGAGGACCA ATTGTTAACA CAACAAAATA AGTGTTCAGA GGAAGATATC 
ATTGAAGATC ATGGCTTAAA CCACACAGAG TCTAACGACC TGGAGAAGAG GCTGAAAAAG 
TTGGAGGAAA AATTCAAACA TGAGATGCAA GCAAAGGAAG AACTGGAAAA TAAATGCCGA 
ATAGCAAATC AGCGGCTGGA GAAATTGTCC AAAGATCTTG AAGAAGAAGT GAATGCGAGA 
CAGGAGGCGG AAGACAACCT GAGATCCCTG GAGAAAGAGA AAGTTTTACT GAAACACCAG 
CGTACACAGA GCGTAAGAAA AGCTGGTCTA GAGACCGACA GAAAACGGCT GCTGGAGAAT 
GAAGTAAGCA GCCTGAAAGA GCAGCTTGCC GAACTGAAGA AAAAGAACCA GATCTCTCAT 
CTGTCTGCTG AGAAGAACAT TCACCTGGAA AGACAGCTGG AGGAGGTGAG TGCCAAGCTT 
CAGGCCGAGC TGGAGGAGTC GGAGAGGCTG AAGAAGGCCC AGATCGAGGC CTTTAGACAG 
TCCCAGCAGC TGGAGCTTTC CCTCAGGGAG CTGCAGGAGC GTCTGGCCCA GCTGGAGAAC 
AGCAGGCTGG TTCTGGAGCA GGACAAACTC AGCCTGCAGA CCTCGCTAGA GCTGGAGAAA 
CGAGAGAGAA ACGCCGGCTC AGAGACCATC ACTGACCTGC AGGGTCGTAT CTATGGACTG 
GAGGGTGAGT TGAAGCATAT AAAGAGTTCT CTATCAAAAG CTGAAGTTGA GAAAAGACAA 
TTACAAGAGG ATCTTACAAC CCTGGAAAAG GAGAAGAATA AGCAGGAGAT CGACCTCTCC 
TTCAAACTCA AAGCAGTTCA GCAGAGTCTG GAGCAGGAGG AGGCTGAACA TAAAACCACT 
AAAGCCAGAT TGGCCGACAA CAACAAAATC AACCAATCCA TAGAGGCAAA GTCTGAGACC 
CTGAAAGACA TGGAACATAA GCTGTTGGAA GAACGATCTG CGAAGCAACA ACTGGAAAAC 
AGACTGATGC AGCTGGAGAA GGAAAACTCT GTATTAGACT GTGATTACAA ACAAGCCAAA 
CATGAGCTTC AGGAGCTCCG CTCGCTCAAA GAAAATCTCA CAGAGCAGGT GGAGGTGCTG 
AATGTGCGGG TGCAGCAGGA GACACAGAGG AAAACTCTCT GTCAAGGTGA TCTCAAAGTT 
CAGAGGCAGG AGATCAACAG CCTGCGGTCA TCAGAACAAC AGCTCAAACA AGAACTCAAC 
CATCTGCTGG AGCTCAAACT CACACTGGAG AAACAAAACC AGGAGCTGTC CAAGGAACGA 
GAAGAATCTG AAAAACAGCT GAAGGAGATG AAGGACCAGC TGGAGGCTGA GCAGTACTTC 
ACGAAACTGT ACAAGACTCA GATTCGAGAG CTGAAAGAGG AAAGCGATGA AAAGGTTAAA 
CTCTACAAAG ATGCTCAGCA GAGAATAGAG GACCTGCAGG AAGAGAGGGA CTCGTTAGCG 
TCTCAGTTGG AGGTGAGCCT GACGAAGGCT GACTCTGAAC AGTTGGCTCG CATCACCGTT 
GAAGAGCAAT ACTCGGACCT GGAGAAAGAG AAGATCATGA AGGAACTGGA GATAAAGGAC 
ATGATCGCCA GACACCGGCA GGATCTTGCA GAAAAGGACG GTACCATCAA CTCGCTGGAG 
GAGTCAAACC GTACGCTGAC TGTGGATGTT GCCAACCTGG CCAGTGAGAA AGAGGAGCTC 
AACAATAAAC TGAAGCACAT ACAGCAAAAG CTGGAGAAGA TCAAAGAAGA AGAGAAACAG 
ATGAAATCTC TCACAGTGTC GTATGAGAAG CAGATACAGG TGGAGAAAAC ACTCAAGATT 
CAGGCTATAA ACAAGCTGGC GGAGGTCATG AAGAAGACGG ACGGCAGGCC ACACCAAATC 
AACAACACAG ACATCCGCAG GAAGGAGAAG GAGATCAGAC AACTCCAGCT GCAGCTCCGC 
ACGGAGAAAG AAAAGCTTAA CCACTCCATC ATCAAATACC AGAGAGAAAT CAACGACATG 
CAGGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207926.1
CDS	589..3837
Translation	MSTGAGKRIESRVRKLETLIKNQKSPLNLESLLDSVTALALDLNHPALRKNKNIDAFLSRYEKAVCNLKELQVKLDDFDRVKLIGRGAFGAVQLVRHKVSQQVYAMKQLSKFEMVKRSDSAFFWEERDIMAFSQSPWIVQLCCAFQDEKYLYLVMEFMPGGDLVTLTSNYDIPEEWAQFYTAEVVLALDAIHSLGFIHRDIKPDNMLLDRNGHLKLADFGTCMKMDSTGMVRCDTAVGTPDYISPEVLMSQGGTGYYGRECDWWSVGVFIYELLVGDTPFYSESLVGTYGKIMDHKNSLTFPDDIEMSKNAKDLICAFLSSREVRLGRTGVDEIKCHPFFKNDQWTFDTIRDTMAPVVPELSSDIDTSNFDDDIKDDPIGTETFPPPRAFAGNQLPFVGFTYFREDQLLTQQNKCSEEDIIEDHGLNHTESNDLEKRLKKLEEKFKHEMQAKEELENKCRIANQRLEKLSKDLEEEVNARQEAEDNLRSLEKEKVLLKHQRTQSVRKAGLETDRKRLLENEVSSLKEQLAELKKKNQISHLSAEKNIHLERQLEEVSAKLQAELEESERLKKAQIEAFRQSQQLELSLRELQERLAQLENSRLVLEQDKLSLQTSLELEKRERNAGSETITDLQGRIYGLEGELKHIKSSLSKAEVEKRQLQEDLTTLEKEKNKQEIDLSFKLKAVQQSLEQEEAEHKTTKARLADNNKINQSIEAKSETLKDMEHKLLEERSAKQQLENRLMQLEKENSVLDCDYKQAKHELQELRSLKENLTEQVEVLNVRVQQETQRKTLCQGDLKVQRQEINSLRSSEQQLKQELNHLLELKLTLEKQNQELSKEREESEKQLKEMKDQLEAEQYFTKLYKTQIRELKEESDEKVKLYKDAQQRIEDLQEERDSLASQLEVSLTKADSEQLARITVEEQYSDLEKEKIMKELEIKDMIARHRQDLAEKDGTINSLEESNRTLTVDVANLASEKEELNNKLKHIQQKLEKIKEEEKQMKSLTVSYEKQIQVEKTLKIQAINKLAEVMKKTDGRPHQINNTDIRRKEKEIRQLQLQLRTEKEKLNHSIIKYQREINDMQA

Target ORF information:

RefSeq Version	XM_017352437.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352437.1

ORF Insert Sequence:

ATGTCGACCG GAGCGGGGAA GAGAATAGAG AGCCGGGTGA GAAAACTAGA GACGCTGATC 
AAGAACCAGA AATCACCGTT GAACTTGGAG AGTTTGCTGG ATTCAGTAAC TGCCCTTGCC 
CTTGATCTGA ACCATCCAGC ACTGAGAAAG AACAAAAACA TAGATGCCTT CTTAAGCAGA 
TATGAAAAAG CCGTGTGCAA CCTCAAGGAA CTACAGGTGA AGCTGGATGA TTTTGATCGA 
GTCAAGCTGA TCGGTCGTGG AGCGTTTGGC GCAGTGCAGC TGGTACGGCA CAAAGTATCT 
CAACAAGTTT ATGCCATGAA GCAGCTCAGC AAGTTTGAGA TGGTCAAGCG CTCTGACTCC 
GCCTTTTTCT GGGAGGAGCG TGACATCATG GCTTTCTCCC AGAGCCCTTG GATAGTTCAG 
CTGTGCTGTG CATTCCAAGA TGAAAAGTAC CTTTATTTGG TGATGGAGTT CATGCCAGGT 
GGAGATCTGG TGACTCTGAC CAGCAACTAT GACATCCCAG AGGAATGGGC TCAATTCTAT 
ACGGCAGAAG TGGTGCTGGC ACTCGACGCC ATCCATTCTC TGGGCTTCAT CCACAGAGAC 
ATCAAACCAG ACAACATGCT GCTGGACCGC AATGGACACC TCAAACTCGC AGACTTTGGC 
ACATGCATGA AGATGGACTC GACTGGTATG GTACGCTGTG ACACTGCTGT TGGGACACCT 
GATTACATTT CTCCAGAGGT TTTAATGTCT CAAGGAGGGA CTGGTTATTA CGGTCGCGAG 
TGTGACTGGT GGTCTGTTGG TGTTTTTATT TATGAGCTTC TAGTGGGCGA CACTCCATTT 
TATTCCGAGT CTCTGGTGGG CACATATGGA AAGATCATGG ATCACAAGAA CAGCTTAACT 
TTCCCAGATG ATATTGAAAT GTCAAAGAAT GCCAAAGACC TCATCTGTGC CTTTCTGAGT 
AGCAGGGAGG TGAGGCTTGG CCGAACTGGA GTGGATGAAA TAAAATGTCA CCCCTTCTTT 
AAGAATGATC AGTGGACCTT CGACACCATA CGAGACACCA TGGCTCCAGT GGTGCCAGAG 
CTGAGTAGCG ATATTGACAC CAGTAACTTT GATGATGACA TTAAGGATGA TCCGATAGGA 
ACAGAGACGT TCCCTCCGCC CCGTGCCTTC GCAGGGAACC AGCTACCATT TGTGGGTTTC 
ACCTATTTCA GGGAGGACCA ATTGTTAACA CAACAAAATA AGTGTTCAGA GGAAGATATC 
ATTGAAGATC ATGGCTTAAA CCACACAGAG TCTAACGACC TGGAGAAGAG GCTGAAAAAG 
TTGGAGGAAA AATTCAAACA TGAGATGCAA GCAAAGGAAG AACTGGAAAA TAAATGCCGA 
ATAGCAAATC AGCGGCTGGA GAAATTGTCC AAAGATCTTG AAGAAGAAGT GAATGCGAGA 
CAGGAGGCGG AAGACAACCT GAGATCCCTG GAGAAAGAGA AAGTTTTACT GAAACACCAG 
CGTACACAGA GCGTAAGAAA AGCTGGTCTA GAGACCGACA GAAAACGGCT GCTGGAGAAT 
GAAGTAAGCA GCCTGAAAGA GCAGCTTGCC GAACTGAAGA AAAAGAACCA GATCTCTCAT 
CTGTCTGCTG AGAAGAACAT TCACCTGGAA AGACAGCTGG AGGAGGTGAG TGCCAAGCTT 
CAGGCCGAGC TGGAGGAGTC GGAGAGGCTG AAGAAGGCCC AGATCGAGGC CTTTAGACAG 
TCCCAGCAGC TGGAGCTTTC CCTCAGGGAG CTGCAGGAGC GTCTGGCCCA GCTGGAGAAC 
AGCAGGCTGG TTCTGGAGCA GGACAAACTC AGCCTGCAGA CCTCGCTAGA GCTGGAGAAA 
CGAGAGAGAA ACGCCGGCTC AGAGACCATC ACTGACCTGC AGGGTCGTAT CTATGGACTG 
GAGGGTGAGT TGAAGCATAT AAAGAGTTCT CTATCAAAAG CTGAAGTTGA GAAAAGACAA 
TTACAAGAGG ATCTTACAAC CCTGGAAAAG GAGAAGAATA AGCAGGAGAT CGACCTCTCC 
TTCAAACTCA AAGCAGTTCA GCAGAGTCTG GAGCAGGAGG AGGCTGAACA TAAAACCACT 
AAAGCCAGAT TGGCCGACAA CAACAAAATC AACCAATCCA TAGAGGCAAA GTCTGAGACC 
CTGAAAGACA TGGAACATAA GCTGTTGGAA GAACGATCTG CGAAGCAACA ACTGGAAAAC 
AGACTGATGC AGCTGGAGAA GGAAAACTCT GTATTAGACT GTGATTACAA ACAAGCCAAA 
CATGAGCTTC AGGAGCTCCG CTCGCTCAAA GAAAATCTCA CAGAGCAGGT GGAGGTGCTG 
AATGTGCGGG TGCAGCAGGA GACACAGAGG AAAACTCTCT GTCAAGGTGA TCTCAAAGTT 
CAGAGGCAGG AGATCAACAG CCTGCGGTCA TCAGAACAAC AGCTCAAACA AGAACTCAAC 
CATCTGCTGG AGCTCAAACT CACACTGGAG AAACAAAACC AGGAGCTGTC CAAGGAACGA 
GAAGAATCTG AAAAACAGCT GAAGGAGATG AAGGACCAGC TGGAGGCTGA GCAGTACTTC 
ACGAAACTGT ACAAGACTCA GATTCGAGAG CTGAAAGAGG AAAGCGATGA AAAGGTTAAA 
CTCTACAAAG ATGCTCAGCA GAGAATAGAG GACCTGCAGG AAGAGAGGGA CTCGTTAGCG 
TCTCAGTTGG AGGTGAGCCT GACGAAGGCT GACTCTGAAC AGTTGGCTCG CATCACCGTT 
GAAGAGCAAT ACTCGGACCT GGAGAAAGAG AAGATCATGA AGGAACTGGA GATAAAGGAC 
ATGATCGCCA GACACCGGCA GGATCTTGCA GAAAAGGACG GTACCATCAA CTCGCTGGAG 
GAGTCAAACC GTACGCTGAC TGTGGATGTT GCCAACCTGG CCAGTGAGAA AGAGGAGCTC 
AACAATAAAC TGAAGCACAT ACAGCAAAAG CTGGAGAAGA TCAAAGAAGA AGAGAAACAG 
ATGAAATCTC TCACAGTGTC GTATGAGAAG CAGATACAGG TGGAGAAAAC ACTCAAGATT 
CAGGCTATAA ACAAGCTGGC GGAGGTCATG AAGAAGACGG ACGGCAGGCC ACACCAAATC 
AACAACACAG ACATCCGCAG GAAGGAGAAG GAGATCAGAC AACTCCAGCT GCAGCTCCGC 
ACGGAGAAAG AAAAGCTTAA CCACTCCATC ATCAAATACC AGAGAGAAAT CAACGACATG 
CAGGCTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rock2b ( NM_001093747.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001087216.1 Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), mRNA.

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CloneID	ODa00028
Clone ID Related Accession (Same CDS sequence)	NM_001093747.2
Accession Version	NM_001093747.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2018-11-23
Organism	Danio rerio(zebrafish)
Product	rho-associated, coiled-coil containing protein kinase 2b
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from BX571790.8 and AL929220.13. On Aug 7, 2010 this sequence version replaced NM_001093747.1. ##Evidence-Data-START## RNAseq introns :: mixed/partial sample support SAMEA3505381, SAMEA3505388 [ECO:0000350] ##Evidence-Data-END##

ATGTCGACCG GAGCGGGGAA GAGAATAGAG AGCCGGGTGA GAAAACTAGA GACGCTGATC 
AAGAACCAGA AATCACCGTT GAACTTGGAG AGTTTGCTGG ATTCAGTAAC TGCCCTTGCC 
CTTGATCTGA ACCATCCAGC ACTGAGAAAG AACAAAAACA TAGATGCCTT CTTAAGCAGA 
TATGAAAAAG CCGTGTGCAA CCTCAAGGAA CTACAGGTGA AGCTGGATGA TTTTGATCGA 
GTCAAGCTGA TCGGTCGTGG AGCGTTTGGC GCAGTGCAGC TGGTACGGCA CAAAGTATCT 
CAACAAGTTT ATGCCATGAA GCAGCTCAGC AAGTTTGAGA TGGTCAAGCG CTCTGACTCC 
GCCTTTTTCT GGGAGGAGCG TGACATCATG GCTTTCTCCC AGAGCCCTTG GATAGTTCAG 
CTGTGCTGTG CATTCCAAGA TGAAAAGTAC CTTTATTTGG TGATGGAGTT CATGCCAGGT 
GGAGATCTGG TGACTCTGAC CAGCAACTAT GACATCCCAG AGGAATGGGC TCAATTCTAT 
ACGGCAGAAG TGGTGCTGGC ACTCGACGCC ATCCATTCTC TGGGCTTCAT CCACAGAGAC 
ATCAAACCAG ACAACATGCT GCTGGACCGC AATGGACACC TCAAACTCGC AGACTTTGGC 
ACATGCATGA AGATGGACTC GACTGGTATG GTACGCTGTG ACACTGCTGT TGGGACACCT 
GATTACATTT CTCCAGAGGT TTTAATGTCT CAAGGAGGGA CTGGTTATTA CGGTCGCGAG 
TGTGACTGGT GGTCTGTTGG TGTTTTTATT TATGAGCTTC TAGTGGGCGA CACTCCATTT 
TATTCCGAGT CTCTGGTGGG CACATATGGA AAGATCATGG ATCACAAGAA CAGCTTAACT 
TTCCCAGATG ATATTGAAAT GTCAAAGAAT GCCAAAGACC TCATCTGTGC CTTTCTGAGT 
AGCAGGGAGG TGAGGCTTGG CCGAACTGGA GTGGATGAAA TAAAATGTCA CCCCTTCTTT 
AAGAATGATC AGTGGACCTT CGACACCATA CGAGACACCA TGGCTCCAGT GGTGCCAGAG 
CTGAGTAGCG ATATTGACAC CAGTAACTTT GATGATGACA TTAAGGATGA TCCGATAGGA 
ACAGAGACGT TCCCTCCGCC CCGTGCCTTC GCAGGGAACC AGCTACCATT TGTGGGTTTC 
ACCTATTTCA GGGAGGACCA ATTGTTAACA CAACAAAATA AGTGTTCAGA GGAAGATATC 
ATTGAAGATC ATGGCTTAAA CCACACAGAG TCTAACGACC TGGAGAAGAG GCTGAAAAAG 
TTGGAGGAAA AATTCAAACA TGAGATGCAA GCAAAGGAAG AACTGGAAAA TAAATGCCGA 
ATAGCAAATC AGCGGCTGGA GAAATTGTCC AAAGATCTTG AAGAAGAAGT GAATGCGAGA 
CAGGAGGCGG AAGACAACCT GAGATCCCTG GAGAAAGAGA AAGTTTTACT GAAACACCAG 
CGTACACAGA GCGTAAGAAA AGCTGGTCTA GAGACCGACA GAAAACGGCT GCTGGAGAAT 
GAAGTAAGCA GCCTGAAAGA GCAGCTTGCC GAACTGAAGA AAAAGAACCA GATCTCTCAT 
CTGTCTGCTG AGAAGAACAT TCACCTGGAA AGACAGCTGG AGGAGGTGAG TGCCAAGCTT 
CAGGCCGAGC TGGAGGAGTC GGAGAGGCTG AAGAAGGCCC AGATCGAGGC CTTTAGACAG 
TCCCAGCAGC TGGAGCTTTC CCTCAGGGAG CTGCAGGAGC GTCTGGCCCA GCTGGAGAAC 
AGCAGGCTGG TTCTGGAGCA GGACAAACTC AGCCTGCAGA CCTCGCTAGA GCTGGAGAAA 
CGAGAGAGAA ACGCCGGCTC AGAGACCATC ACTGACCTGC AGGGTCGTAT CTATGGACTG 
GAGGGTGAGT TGAAGCATAT AAAGAGTTCT CTATCAAAAG CTGAAGTTGA GAAAAGACAA 
TTACAAGAGG ATCTTACAAC CCTGGAAAAG GAGAAGAATA AGCAGGAGAT CGACCTCTCC 
TTCAAACTCA AAGCAGTTCA GCAGAGTCTG GAGCAGGAGG AGGCTGAACA TAAAACCACT 
AAAGCCAGAT TGGCCGACAA CAACAAAATC AACCAATCCA TAGAGGCAAA GTCTGAGACC 
CTGAAAGACA TGGAACATAA GCTGTTGGAA GAACGATCTG CGAAGCAACA ACTGGAAAAC 
AGACTGATGC AGCTGGAGAA GGAAAACTCT GTATTAGACT GTGATTACAA ACAAGCCAAA 
CATGAGCTTC AGGAGCTCCG CTCGCTCAAA GAAAATCTCA CAGAGCAGGT GGAGGTGCTG 
AATGTGCGGG TGCAGCAGGA GACACAGAGG AAAACTCTCT GTCAAGGTGA TCTCAAAGTT 
CAGAGGCAGG AGATCAACAG CCTGCGGTCA TCAGAACAAC AGCTCAAACA AGAACTCAAC 
CATCTGCTGG AGCTCAAACT CACACTGGAG AAACAAAACC AGGAGCTGTC CAAGGAACGA 
GAAGAATCTG AAAAACAGCT GAAGGAGATG AAGGACCAGC TGGAGGCTGA GCAGTACTTC 
ACGAAACTGT ACAAGACTCA GATTCGAGAG CTGAAAGAGG AAAGCGATGA AAAGGTTAAA 
CTCTACAAAG ATGCTCAGCA GAGAATAGAG GACCTGCAGG AAGAGAGGGA CTCGTTAGCG 
TCTCAGTTGG AGGTGAGCCT GACGAAGGCT GACTCTGAAC AGTTGGCTCG CATCACCGTT 
GAAGAGCAAT ACTCGGACCT GGAGAAAGAG AAGATCATGA AGGAACTGGA GATAAAGGAC 
ATGATCGCCA GACACCGGCA GGATCTTGCA GAAAAGGACG GTACCATCAA CTCGCTGGAG 
GAGTCAAACC GTACGCTGAC TGTGGATGTT GCCAACCTGG CCAGTGAGAA AGAGGAGCTC 
AACAATAAAC TGAAGCACAT ACAGCAAAAG CTGGAGAAGA TCAAAGAAGA AGAGAAACAG 
ATGAAATCTC TCACAGTGTC GTATGAGAAG CAGATACAGG TGGAGAAAAC ACTCAAGATT 
CAGGCTATAA ACAAGCTGGC GGAGGTCATG AAGAAGACGG ACGGCAGGCC ACACCAAATC 
AACAACACAG ACATCCGCAG GAAGGAGAAG GAGATCAGAC AACTCCAGCT GCAGCTCCGC 
ACGGAGAAAG AAAAGCTTAA CCACTCCATC ATCAAATACC AGAGAGAAAT CAACGACATG 
CAGGCTATGA TAGCAGAGGA GTCTCAGGTG CGTCTGGAGA TGCAGATGTC TCTGGACAGT 
AAAGACAGTG ATATTGAGCG TCTGCGCAGT CAACTCACGT CTCTGAGCAT TCACTCTCTG 
GACACCACCA GCATCAGCAG CATCGGCAAT GACCTGGATT CTGACGAGGC ATATCCAGCC 
GCAGAGTCCG GTCTTGAAGG ATGGCTCTCA ATGCCTGCCA AAAACACCAA ACGGTTTGGA 
TGGGATAAGA AGTATGTGGT CGTGAGCAGC AAGAAGATCC TGTTTTATGA CTCTGAACAA 
GACCGAGAGC AGTCCAGTCC ATTCATGATC CTGGACATTG ACAAACTCTT CCACGTGAGA 
CCCGTCACTC AGACAGATGT TTACCGCGCA GATCCCAGAG AAATCCCTCG CATCTTCCAG 
ATACTATATG CGAATGAAGG AGAGAGCAAG AAAGAGGAGC TGCCCGTGGA GTCGTTATCT 
GTGACTGAGA GGTCACTGTG TATCCCACAC AAGGGTCACG AGTTTGTTCT GACGCTTTAT 
CACTTCCCCT CGAGCTGCGA GGTCTGTCCA CGACCCCTAT GGCACGTCTT CAGACCTCCG 
CCGGCTCTCG AGTGCAGACG CTGTAGAGTC AAGTGCCATA AAGACCACAT CGATCGCAAA 
GAAGAGGTCC TCGCTCCATG TAGAGTGAAT TACGACATGT CCACAGCGAA AGAGATGCTG 
CTGCTGGCAA ACTCCACAGA GGAGCAGAAG AAGTGGGTCA GTCGGCTGCT CAAGAGGGTT 
CCGCGTAAAC CCTCCATCGC CCACTCTTCC AGCATAGCCA TATCCGCTGC GCCCTCAGAG 
GCAACACCCA CTACCCTTTC CCCTCAGCCG TCGCCTCGCC TCGCTCAGTC TTCACCCAGA 
CTTTCACACC GAGCAGCCGT CAAAGTGCAC TCTACACGAC AGCAGCCCTC CAGCAAGAAC 
AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001087216.1
CDS	1..4206
Translation	MSTGAGKRIESRVRKLETLIKNQKSPLNLESLLDSVTALALDLNHPALRKNKNIDAFLSRYEKAVCNLKELQVKLDDFDRVKLIGRGAFGAVQLVRHKVSQQVYAMKQLSKFEMVKRSDSAFFWEERDIMAFSQSPWIVQLCCAFQDEKYLYLVMEFMPGGDLVTLTSNYDIPEEWAQFYTAEVVLALDAIHSLGFIHRDIKPDNMLLDRNGHLKLADFGTCMKMDSTGMVRCDTAVGTPDYISPEVLMSQGGTGYYGRECDWWSVGVFIYELLVGDTPFYSESLVGTYGKIMDHKNSLTFPDDIEMSKNAKDLICAFLSSREVRLGRTGVDEIKCHPFFKNDQWTFDTIRDTMAPVVPELSSDIDTSNFDDDIKDDPIGTETFPPPRAFAGNQLPFVGFTYFREDQLLTQQNKCSEEDIIEDHGLNHTESNDLEKRLKKLEEKFKHEMQAKEELENKCRIANQRLEKLSKDLEEEVNARQEAEDNLRSLEKEKVLLKHQRTQSVRKAGLETDRKRLLENEVSSLKEQLAELKKKNQISHLSAEKNIHLERQLEEVSAKLQAELEESERLKKAQIEAFRQSQQLELSLRELQERLAQLENSRLVLEQDKLSLQTSLELEKRERNAGSETITDLQGRIYGLEGELKHIKSSLSKAEVEKRQLQEDLTTLEKEKNKQEIDLSFKLKAVQQSLEQEEAEHKTTKARLADNNKINQSIEAKSETLKDMEHKLLEERSAKQQLENRLMQLEKENSVLDCDYKQAKHELQELRSLKENLTEQVEVLNVRVQQETQRKTLCQGDLKVQRQEINSLRSSEQQLKQELNHLLELKLTLEKQNQELSKEREESEKQLKEMKDQLEAEQYFTKLYKTQIRELKEESDEKVKLYKDAQQRIEDLQEERDSLASQLEVSLTKADSEQLARITVEEQYSDLEKEKIMKELEIKDMIARHRQDLAEKDGTINSLEESNRTLTVDVANLASEKEELNNKLKHIQQKLEKIKEEEKQMKSLTVSYEKQIQVEKTLKIQAINKLAEVMKKTDGRPHQINNTDIRRKEKEIRQLQLQLRTEKEKLNHSIIKYQREINDMQAMIAEESQVRLEMQMSLDSKDSDIERLRSQLTSLSIHSLDTTSISSIGNDLDSDEAYPAAESGLEGWLSMPAKNTKRFGWDKKYVVVSSKKILFYDSEQDREQSSPFMILDIDKLFHVRPVTQTDVYRADPREIPRIFQILYANEGESKKEELPVESLSVTERSLCIPHKGHEFVLTLYHFPSSCEVCPRPLWHVFRPPPALECRRCRVKCHKDHIDRKEEVLAPCRVNYDMSTAKEMLLLANSTEEQKKWVSRLLKRVPRKPSIAHSSSIAISAAPSEATPTTLSPQPSPRLAQSSPRLSHRAAVKVHSTRQQPSSKNR

Target ORF information:

RefSeq Version	NM_001093747.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001093747.2

ORF Insert Sequence:

ATGTCGACCG GAGCGGGGAA GAGAATAGAG AGCCGGGTGA GAAAACTAGA GACGCTGATC 
AAGAACCAGA AATCACCGTT GAACTTGGAG AGTTTGCTGG ATTCAGTAAC TGCCCTTGCC 
CTTGATCTGA ACCATCCAGC ACTGAGAAAG AACAAAAACA TAGATGCCTT CTTAAGCAGA 
TATGAAAAAG CCGTGTGCAA CCTCAAGGAA CTACAGGTGA AGCTGGATGA TTTTGATCGA 
GTCAAGCTGA TCGGTCGTGG AGCGTTTGGC GCAGTGCAGC TGGTACGGCA CAAAGTATCT 
CAACAAGTTT ATGCCATGAA GCAGCTCAGC AAGTTTGAGA TGGTCAAGCG CTCTGACTCC 
GCCTTTTTCT GGGAGGAGCG TGACATCATG GCTTTCTCCC AGAGCCCTTG GATAGTTCAG 
CTGTGCTGTG CATTCCAAGA TGAAAAGTAC CTTTATTTGG TGATGGAGTT CATGCCAGGT 
GGAGATCTGG TGACTCTGAC CAGCAACTAT GACATCCCAG AGGAATGGGC TCAATTCTAT 
ACGGCAGAAG TGGTGCTGGC ACTCGACGCC ATCCATTCTC TGGGCTTCAT CCACAGAGAC 
ATCAAACCAG ACAACATGCT GCTGGACCGC AATGGACACC TCAAACTCGC AGACTTTGGC 
ACATGCATGA AGATGGACTC GACTGGTATG GTACGCTGTG ACACTGCTGT TGGGACACCT 
GATTACATTT CTCCAGAGGT TTTAATGTCT CAAGGAGGGA CTGGTTATTA CGGTCGCGAG 
TGTGACTGGT GGTCTGTTGG TGTTTTTATT TATGAGCTTC TAGTGGGCGA CACTCCATTT 
TATTCCGAGT CTCTGGTGGG CACATATGGA AAGATCATGG ATCACAAGAA CAGCTTAACT 
TTCCCAGATG ATATTGAAAT GTCAAAGAAT GCCAAAGACC TCATCTGTGC CTTTCTGAGT 
AGCAGGGAGG TGAGGCTTGG CCGAACTGGA GTGGATGAAA TAAAATGTCA CCCCTTCTTT 
AAGAATGATC AGTGGACCTT CGACACCATA CGAGACACCA TGGCTCCAGT GGTGCCAGAG 
CTGAGTAGCG ATATTGACAC CAGTAACTTT GATGATGACA TTAAGGATGA TCCGATAGGA 
ACAGAGACGT TCCCTCCGCC CCGTGCCTTC GCAGGGAACC AGCTACCATT TGTGGGTTTC 
ACCTATTTCA GGGAGGACCA ATTGTTAACA CAACAAAATA AGTGTTCAGA GGAAGATATC 
ATTGAAGATC ATGGCTTAAA CCACACAGAG TCTAACGACC TGGAGAAGAG GCTGAAAAAG 
TTGGAGGAAA AATTCAAACA TGAGATGCAA GCAAAGGAAG AACTGGAAAA TAAATGCCGA 
ATAGCAAATC AGCGGCTGGA GAAATTGTCC AAAGATCTTG AAGAAGAAGT GAATGCGAGA 
CAGGAGGCGG AAGACAACCT GAGATCCCTG GAGAAAGAGA AAGTTTTACT GAAACACCAG 
CGTACACAGA GCGTAAGAAA AGCTGGTCTA GAGACCGACA GAAAACGGCT GCTGGAGAAT 
GAAGTAAGCA GCCTGAAAGA GCAGCTTGCC GAACTGAAGA AAAAGAACCA GATCTCTCAT 
CTGTCTGCTG AGAAGAACAT TCACCTGGAA AGACAGCTGG AGGAGGTGAG TGCCAAGCTT 
CAGGCCGAGC TGGAGGAGTC GGAGAGGCTG AAGAAGGCCC AGATCGAGGC CTTTAGACAG 
TCCCAGCAGC TGGAGCTTTC CCTCAGGGAG CTGCAGGAGC GTCTGGCCCA GCTGGAGAAC 
AGCAGGCTGG TTCTGGAGCA GGACAAACTC AGCCTGCAGA CCTCGCTAGA GCTGGAGAAA 
CGAGAGAGAA ACGCCGGCTC AGAGACCATC ACTGACCTGC AGGGTCGTAT CTATGGACTG 
GAGGGTGAGT TGAAGCATAT AAAGAGTTCT CTATCAAAAG CTGAAGTTGA GAAAAGACAA 
TTACAAGAGG ATCTTACAAC CCTGGAAAAG GAGAAGAATA AGCAGGAGAT CGACCTCTCC 
TTCAAACTCA AAGCAGTTCA GCAGAGTCTG GAGCAGGAGG AGGCTGAACA TAAAACCACT 
AAAGCCAGAT TGGCCGACAA CAACAAAATC AACCAATCCA TAGAGGCAAA GTCTGAGACC 
CTGAAAGACA TGGAACATAA GCTGTTGGAA GAACGATCTG CGAAGCAACA ACTGGAAAAC 
AGACTGATGC AGCTGGAGAA GGAAAACTCT GTATTAGACT GTGATTACAA ACAAGCCAAA 
CATGAGCTTC AGGAGCTCCG CTCGCTCAAA GAAAATCTCA CAGAGCAGGT GGAGGTGCTG 
AATGTGCGGG TGCAGCAGGA GACACAGAGG AAAACTCTCT GTCAAGGTGA TCTCAAAGTT 
CAGAGGCAGG AGATCAACAG CCTGCGGTCA TCAGAACAAC AGCTCAAACA AGAACTCAAC 
CATCTGCTGG AGCTCAAACT CACACTGGAG AAACAAAACC AGGAGCTGTC CAAGGAACGA 
GAAGAATCTG AAAAACAGCT GAAGGAGATG AAGGACCAGC TGGAGGCTGA GCAGTACTTC 
ACGAAACTGT ACAAGACTCA GATTCGAGAG CTGAAAGAGG AAAGCGATGA AAAGGTTAAA 
CTCTACAAAG ATGCTCAGCA GAGAATAGAG GACCTGCAGG AAGAGAGGGA CTCGTTAGCG 
TCTCAGTTGG AGGTGAGCCT GACGAAGGCT GACTCTGAAC AGTTGGCTCG CATCACCGTT 
GAAGAGCAAT ACTCGGACCT GGAGAAAGAG AAGATCATGA AGGAACTGGA GATAAAGGAC 
ATGATCGCCA GACACCGGCA GGATCTTGCA GAAAAGGACG GTACCATCAA CTCGCTGGAG 
GAGTCAAACC GTACGCTGAC TGTGGATGTT GCCAACCTGG CCAGTGAGAA AGAGGAGCTC 
AACAATAAAC TGAAGCACAT ACAGCAAAAG CTGGAGAAGA TCAAAGAAGA AGAGAAACAG 
ATGAAATCTC TCACAGTGTC GTATGAGAAG CAGATACAGG TGGAGAAAAC ACTCAAGATT 
CAGGCTATAA ACAAGCTGGC GGAGGTCATG AAGAAGACGG ACGGCAGGCC ACACCAAATC 
AACAACACAG ACATCCGCAG GAAGGAGAAG GAGATCAGAC AACTCCAGCT GCAGCTCCGC 
ACGGAGAAAG AAAAGCTTAA CCACTCCATC ATCAAATACC AGAGAGAAAT CAACGACATG 
CAGGCTATGA TAGCAGAGGA GTCTCAGGTG CGTCTGGAGA TGCAGATGTC TCTGGACAGT 
AAAGACAGTG ATATTGAGCG TCTGCGCAGT CAACTCACGT CTCTGAGCAT TCACTCTCTG 
GACACCACCA GCATCAGCAG CATCGGCAAT GACCTGGATT CTGACGAGGC ATATCCAGCC 
GCAGAGTCCG GTCTTGAAGG ATGGCTCTCA ATGCCTGCCA AAAACACCAA ACGGTTTGGA 
TGGGATAAGA AGTATGTGGT CGTGAGCAGC AAGAAGATCC TGTTTTATGA CTCTGAACAA 
GACCGAGAGC AGTCCAGTCC ATTCATGATC CTGGACATTG ACAAACTCTT CCACGTGAGA 
CCCGTCACTC AGACAGATGT TTACCGCGCA GATCCCAGAG AAATCCCTCG CATCTTCCAG 
ATACTATATG CGAATGAAGG AGAGAGCAAG AAAGAGGAGC TGCCCGTGGA GTCGTTATCT 
GTGACTGAGA GGTCACTGTG TATCCCACAC AAGGGTCACG AGTTTGTTCT GACGCTTTAT 
CACTTCCCCT CGAGCTGCGA GGTCTGTCCA CGACCCCTAT GGCACGTCTT CAGACCTCCG 
CCGGCTCTCG AGTGCAGACG CTGTAGAGTC AAGTGCCATA AAGACCACAT CGATCGCAAA 
GAAGAGGTCC TCGCTCCATG TAGAGTGAAT TACGACATGT CCACAGCGAA AGAGATGCTG 
CTGCTGGCAA ACTCCACAGA GGAGCAGAAG AAGTGGGTCA GTCGGCTGCT CAAGAGGGTT 
CCGCGTAAAC CCTCCATCGC CCACTCTTCC AGCATAGCCA TATCCGCTGC GCCCTCAGAG 
GCAACACCCA CTACCCTTTC CCCTCAGCCG TCGCCTCGCC TCGCTCAGTC TTCACCCAGA 
CTTTCACACC GAGCAGCCGT CAAAGTGCAC TCTACACGAC AGCAGCCCTC CAGCAAGAAC 
AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rock2b ( XM_009294695.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292970.1 Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X4, mRNA.

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CloneID	ODa24918
Clone ID Related Accession (Same CDS sequence)	XM_009294695.2 , XM_009294695.3
Accession Version	XM_009294695.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	rho-associated, coiled-coil containing protein kinase 2b isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009294695.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGACCG GAGCGGGGAA GAGAATAGAG AGCCGGGTGA GAAAACTAGA GACGCTGATC 
AAGAACCAGA AATCACCGTT GAACTTGGAG AGTTTGCTGG ATTCAGTAAC TGCCCTTGCC 
CTTGATCTGA ACCATCCAGC ACTGAGAAAG AACAAAAACA TAGATGCCTT CTTAAGCAGA 
TATGAAAAAG CCGTGTGCAA CCTCAAGGAA CTACAGGTGA AGCTGGATGA TTTTGATCGA 
GTCAAGCTGA TCGGTCGTGG AGCGTTTGGC GCAGTGCAGC TGGTACGGCA CAAAGTATCT 
CAACAAGTTT ATGCCATGAA GCAGCTCAGC AAGTTTGAGA TGGTCAAGCG CTCTGACTCC 
GCCTTTTTCT GGGAGGAGCG TGACATCATG GCTTTCTCCC AGAGCCCTTG GATAGTTCAG 
CTGTGCTGTG CATTCCAAGA TGAAAAGTAC CTTTATTTGG TGATGGAGTT CATGCCAGGT 
GGAGATCTGG TGACTCTGAC CAGCAACTAT GACATCCCAG AGGAATGGGC TCAATTCTAT 
ACGGCAGAAG TGGTGCTGGC ACTCGACGCC ATCCATTCTC TGGGCTTCAT CCACAGAGAC 
ATCAAACCAG ACAACATGCT GCTGGACCGC AATGGACACC TCAAACTCGC AGACTTTGGC 
ACATGCATGA AGATGGACTC GACTGGTATG GTACGCTGTG ACACTGCTGT TGGGACACCT 
GATTACATTT CTCCAGAGGT TTTAATGTCT CAAGGAGGGA CTGGTTATTA CGGTCGCGAG 
TGTGACTGGT GGTCTGTTGG TGTTTTTATT TATGAGCTTC TAGTGGGCGA CACTCCATTT 
TATTCCGAGT CTCTGGTGGG CACATATGGA AAGATCATGG ATCACAAGAA CAGCTTAACT 
TTCCCAGATG ATATTGAAAT GTCAAAGAAT GCCAAAGACC TCATCTGTGC CTTTCTGAGT 
AGCAGGGAGG TGAGGCTTGG CCGAACTGGA GTGGATGAAA TAAAATGTCA CCCCTTCTTT 
AAGAATGATC AGTGGACCTT CGACACCATA CGAGACACCA TGGCTCCAGT GGTGCCAGAG 
CTGAGTAGCG ATATTGACAC CAGTAACTTT GATGATGACA TTAAGGATGA TCCGATAGGA 
ACAGAGACGT TCCCTCCGCC CCGTGCCTTC GCAGGGAACC AGCTACCATT TGTGGGTTTC 
ACCTATTTCA GGGAGGACCA ATTGTTAACA CAACAAAATA AGTGTTCAGA GGAAGATATC 
ATTGAAGATC ATGGCTTAAA CCACACAGAG TCTAACGACC TGGAGAAGAG GCTGAAAAAG 
TTGGAGGAAA AATTCAAACA TGAGATGCAA GCAAAGGAAG AACTGGAAAA TAAATGCCGA 
ATAGCAAATC AGCGGCTGGA GAAATTGTCC AAAGATCTTG AAGAAGAAGT GAATGCGAGA 
CAGGAGGCGG AAGACAACCT GAGATCCCTG GAGAAAGAGA AAGTTTTACT GAAACACCAG 
CGTACACAGA GCGTAAGAAA AGCTGGTCTA GAGACCGACA GAAAACGGCT GCTGGAGAAT 
GAAGTAAGCA GCCTGAAAGA GCAGCTTGCC GAACTGAAGA AAAAGAACCA GATCTCTCAT 
CTGTCTGCTG AGAAGAACAT TCACCTGGAA AGACAGCTGG AGGAGGTGAG TGCCAAGCTT 
CAGGCCGAGC TGGAGGAGTC GGAGAGGCTG AAGAAGGCCC AGATCGAGGC CTTTAGACAG 
TCCCAGCAGC TGGAGCTTTC CCTCAGGGAG CTGCAGGAGC GTCTGGCCCA GCTGGAGAAC 
AGCAGGCTGG TTCTGGAGCA GGACAAACTC AGCCTGCAGA CCTCGCTAGA GCTGGAGAAA 
CGAGAGAGAA ACGCCGGCTC AGAGACCATC ACTGACCTGC AGGGTCGTAT CTATGGACTG 
GAGGGTGAGT TGAAGCATAT AAAGAGTTCT CTATCAAAAG CTGAAGTTGA GAAAAGACAA 
TTACAAGAGG ATCTTACAAC CCTGGAAAAG GAGAAGAATA AGCAGGAGAT CGACCTCTCC 
TTCAAACTCA AAGCAGTTCA GCAGAGTCTG GAGCAGGAGG AGGCTGAACA TAAAACCACT 
AAAGCCAGAT TGGCCGACAA CAACAAAATC AACCAATCCA TAGAGGCAAA GTCTGAGACC 
CTGAAAGACA TGGAACATAA GCTGTTGGAA GAACGATCTG CGAAGCAACA ACTGGAAAAC 
AGACTGATGC AGCTGGAGAA GGAAAACTCT GTATTAGACT GTGATTACAA ACAAGCCAAA 
CATGAGCTTC AGGAGCTCCG CTCGCTCAAA GAAAATCTCA CAGAGCAGGT GGAGGTGCTG 
AATGTGCGGG TGCAGCAGGA GACACAGAGG AAAACTCTCT GTCAAGGTGA TCTCAAAGTT 
CAGAGGCAGG AGATCAACAG CCTGCGGTCA TCAGAACAAC AGCTCAAACA AGAACTCAAC 
CATCTGCTGG AGCTCAAACT CACACTGGAG AAACAAAACC AGGAGCTGTC CAAGGAACGA 
GAAGAATCTG AAAAACAGCT GAAGGAGATG AAGGACCAGC TGGAGGCTGA GCAGTACTTC 
ACGAAACTGT ACAAGACTCA GATTCGAGAG CTGAAAGAGG AAAGCGATGA AAAGGTTAAA 
CTCTACAAAG ATGCTCAGCA GAGAATAGAG GACCTGCAGG AAGAGAGGGA CTCGTTAGCG 
TCTCAGTTGG AGGTGAGCCT GACGAAGGCT GACTCTGAAC AGTTGGCTCG CATCACCGTT 
GAAGAGCAAT ACTCGGACCT GGAGAAAGAG AAGATCATGA AGGAACTGGA GATAAAGGAC 
ATGATCGCCA GACACCGGCA GGATCTTGCA GAAAAGGACG GTACCATCAA CTCGCTGGAG 
GAGTCAAACC GTACGCTGAC TGTGGATGTT GCCAACCTGG CCAGTGAGAA AGAGGAGCTC 
AACAATAAAC TGAAGCACAT ACAGCAAAAG CTGGAGAAGA TCAAAGAAGA AGAGAAACAG 
ATGAAATCTC TCACAGTGTC GTATGAGAAG CAGATACAGG TGGAGAAAAC ACTCAAGATT 
CAGGCTATAA ACAAGCTGGC GGAGGTCATG AAGAAGACGG ACGGCAGGCC ACACCAAATC 
AACAACACAG ACATCCGCAG GAAGGAGAAG GAGATCAGAC AACTCCAGCT GCAGCTCCGC 
ACGGAGAAAG AAAAGCTTAA CCACTCCATC ATCAAATACC AGAGAGAAAT CAACGACATG 
CAGGCTATGA TAGCAGAGGA GTCTCAGGTG CGTCTGGAGA TGCAGATGTC TCTGGACAGT 
AAAGACAGTG ATATTGAGCG TCTGCGCAGT CAACTCACGT CTCTGAGCAT TCACTCTCTG 
GACACCACCA GCATCAGCAG CATCGGCAAT GACCTGGATT CTGACGAGGC ATATCCAGCC 
GCAGAGTCCG GTCTTGAAGG ATGGCTCTCA ATGCCTGCCA AAAACACCAA ACGGTTTGGA 
TGGGATAAGA AGTATGTGGT TGTGAGCAGC AAGAAAATCC TGTTTTATGA CTCTGAACAA 
GACCGAGAGC AGTCCAGTCC ATTCATGATC CTGGACATTG ACAAACTCTT CCACGTGAGA 
CCCGTCACTC AGACAGATGT TTACCGCGCA GATCCCAGAG AAATCCCTCG CATCTTCCAG 
ATACTATATG CGAATGAAGG AGAGAGCAAG AAAGAGGAGC TGCCCGTGGA GTCGTTATCT 
GTGACTGAGA GGTCACTGTG TATCCCACAC AAGGGTCACG AGTTTGTTCT GACGCTTTAT 
CACTTCCCCT CGAGCTGCGA GGTCTGTCCA CGACCCTTAT GGCACGTCTT CAGACCTCCG 
CCGGCTCTCG AGTGCAGACG CTGTAGAGTC AAGTGCCATA AAGACCACAT CGATCGCAAA 
GAAGAGGTCC TCGCTCCATG TAGAGTGAAT TACGACATGT CCACAGCGAA AGAGATGCTG 
CTGCTGGCAA ACTCCACAGA GGAGCAGAAG AAGTGGGTCA GTCGGCTGCT CAAGAGGGTT 
CCGCGTAAAC CTTCCATCGC CCACTCTTCC AGCATAGCCA TATCCGCTGC GCCCTCAGAG 
GCAACACCCA CTACCCTTTC CCCTCAGCCG TCGCCTCGCC TCGCTCAGTC TTCACCCAGA 
CTTTCACACC GAGCAGCCGT CAAAGTGCAC TCTACACGAC AGCAGCCCTC CAGCAAGAAC 
AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292970.1
CDS	578..4783
Translation	MSTGAGKRIESRVRKLETLIKNQKSPLNLESLLDSVTALALDLNHPALRKNKNIDAFLSRYEKAVCNLKELQVKLDDFDRVKLIGRGAFGAVQLVRHKVSQQVYAMKQLSKFEMVKRSDSAFFWEERDIMAFSQSPWIVQLCCAFQDEKYLYLVMEFMPGGDLVTLTSNYDIPEEWAQFYTAEVVLALDAIHSLGFIHRDIKPDNMLLDRNGHLKLADFGTCMKMDSTGMVRCDTAVGTPDYISPEVLMSQGGTGYYGRECDWWSVGVFIYELLVGDTPFYSESLVGTYGKIMDHKNSLTFPDDIEMSKNAKDLICAFLSSREVRLGRTGVDEIKCHPFFKNDQWTFDTIRDTMAPVVPELSSDIDTSNFDDDIKDDPIGTETFPPPRAFAGNQLPFVGFTYFREDQLLTQQNKCSEEDIIEDHGLNHTESNDLEKRLKKLEEKFKHEMQAKEELENKCRIANQRLEKLSKDLEEEVNARQEAEDNLRSLEKEKVLLKHQRTQSVRKAGLETDRKRLLENEVSSLKEQLAELKKKNQISHLSAEKNIHLERQLEEVSAKLQAELEESERLKKAQIEAFRQSQQLELSLRELQERLAQLENSRLVLEQDKLSLQTSLELEKRERNAGSETITDLQGRIYGLEGELKHIKSSLSKAEVEKRQLQEDLTTLEKEKNKQEIDLSFKLKAVQQSLEQEEAEHKTTKARLADNNKINQSIEAKSETLKDMEHKLLEERSAKQQLENRLMQLEKENSVLDCDYKQAKHELQELRSLKENLTEQVEVLNVRVQQETQRKTLCQGDLKVQRQEINSLRSSEQQLKQELNHLLELKLTLEKQNQELSKEREESEKQLKEMKDQLEAEQYFTKLYKTQIRELKEESDEKVKLYKDAQQRIEDLQEERDSLASQLEVSLTKADSEQLARITVEEQYSDLEKEKIMKELEIKDMIARHRQDLAEKDGTINSLEESNRTLTVDVANLASEKEELNNKLKHIQQKLEKIKEEEKQMKSLTVSYEKQIQVEKTLKIQAINKLAEVMKKTDGRPHQINNTDIRRKEKEIRQLQLQLRTEKEKLNHSIIKYQREINDMQAMIAEESQVRLEMQMSLDSKDSDIERLRSQLTSLSIHSLDTTSISSIGNDLDSDEAYPAAESGLEGWLSMPAKNTKRFGWDKKYVVVSSKKILFYDSEQDREQSSPFMILDIDKLFHVRPVTQTDVYRADPREIPRIFQILYANEGESKKEELPVESLSVTERSLCIPHKGHEFVLTLYHFPSSCEVCPRPLWHVFRPPPALECRRCRVKCHKDHIDRKEEVLAPCRVNYDMSTAKEMLLLANSTEEQKKWVSRLLKRVPRKPSIAHSSSIAISAAPSEATPTTLSPQPSPRLAQSSPRLSHRAAVKVHSTRQQPSSKNR

Target ORF information:

RefSeq Version	XM_009294695.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294695.2

ORF Insert Sequence:

ATGTCGACCG GAGCGGGGAA GAGAATAGAG AGCCGGGTGA GAAAACTAGA GACGCTGATC 
AAGAACCAGA AATCACCGTT GAACTTGGAG AGTTTGCTGG ATTCAGTAAC TGCCCTTGCC 
CTTGATCTGA ACCATCCAGC ACTGAGAAAG AACAAAAACA TAGATGCCTT CTTAAGCAGA 
TATGAAAAAG CCGTGTGCAA CCTCAAGGAA CTACAGGTGA AGCTGGATGA TTTTGATCGA 
GTCAAGCTGA TCGGTCGTGG AGCGTTTGGC GCAGTGCAGC TGGTACGGCA CAAAGTATCT 
CAACAAGTTT ATGCCATGAA GCAGCTCAGC AAGTTTGAGA TGGTCAAGCG CTCTGACTCC 
GCCTTTTTCT GGGAGGAGCG TGACATCATG GCTTTCTCCC AGAGCCCTTG GATAGTTCAG 
CTGTGCTGTG CATTCCAAGA TGAAAAGTAC CTTTATTTGG TGATGGAGTT CATGCCAGGT 
GGAGATCTGG TGACTCTGAC CAGCAACTAT GACATCCCAG AGGAATGGGC TCAATTCTAT 
ACGGCAGAAG TGGTGCTGGC ACTCGACGCC ATCCATTCTC TGGGCTTCAT CCACAGAGAC 
ATCAAACCAG ACAACATGCT GCTGGACCGC AATGGACACC TCAAACTCGC AGACTTTGGC 
ACATGCATGA AGATGGACTC GACTGGTATG GTACGCTGTG ACACTGCTGT TGGGACACCT 
GATTACATTT CTCCAGAGGT TTTAATGTCT CAAGGAGGGA CTGGTTATTA CGGTCGCGAG 
TGTGACTGGT GGTCTGTTGG TGTTTTTATT TATGAGCTTC TAGTGGGCGA CACTCCATTT 
TATTCCGAGT CTCTGGTGGG CACATATGGA AAGATCATGG ATCACAAGAA CAGCTTAACT 
TTCCCAGATG ATATTGAAAT GTCAAAGAAT GCCAAAGACC TCATCTGTGC CTTTCTGAGT 
AGCAGGGAGG TGAGGCTTGG CCGAACTGGA GTGGATGAAA TAAAATGTCA CCCCTTCTTT 
AAGAATGATC AGTGGACCTT CGACACCATA CGAGACACCA TGGCTCCAGT GGTGCCAGAG 
CTGAGTAGCG ATATTGACAC CAGTAACTTT GATGATGACA TTAAGGATGA TCCGATAGGA 
ACAGAGACGT TCCCTCCGCC CCGTGCCTTC GCAGGGAACC AGCTACCATT TGTGGGTTTC 
ACCTATTTCA GGGAGGACCA ATTGTTAACA CAACAAAATA AGTGTTCAGA GGAAGATATC 
ATTGAAGATC ATGGCTTAAA CCACACAGAG TCTAACGACC TGGAGAAGAG GCTGAAAAAG 
TTGGAGGAAA AATTCAAACA TGAGATGCAA GCAAAGGAAG AACTGGAAAA TAAATGCCGA 
ATAGCAAATC AGCGGCTGGA GAAATTGTCC AAAGATCTTG AAGAAGAAGT GAATGCGAGA 
CAGGAGGCGG AAGACAACCT GAGATCCCTG GAGAAAGAGA AAGTTTTACT GAAACACCAG 
CGTACACAGA GCGTAAGAAA AGCTGGTCTA GAGACCGACA GAAAACGGCT GCTGGAGAAT 
GAAGTAAGCA GCCTGAAAGA GCAGCTTGCC GAACTGAAGA AAAAGAACCA GATCTCTCAT 
CTGTCTGCTG AGAAGAACAT TCACCTGGAA AGACAGCTGG AGGAGGTGAG TGCCAAGCTT 
CAGGCCGAGC TGGAGGAGTC GGAGAGGCTG AAGAAGGCCC AGATCGAGGC CTTTAGACAG 
TCCCAGCAGC TGGAGCTTTC CCTCAGGGAG CTGCAGGAGC GTCTGGCCCA GCTGGAGAAC 
AGCAGGCTGG TTCTGGAGCA GGACAAACTC AGCCTGCAGA CCTCGCTAGA GCTGGAGAAA 
CGAGAGAGAA ACGCCGGCTC AGAGACCATC ACTGACCTGC AGGGTCGTAT CTATGGACTG 
GAGGGTGAGT TGAAGCATAT AAAGAGTTCT CTATCAAAAG CTGAAGTTGA GAAAAGACAA 
TTACAAGAGG ATCTTACAAC CCTGGAAAAG GAGAAGAATA AGCAGGAGAT CGACCTCTCC 
TTCAAACTCA AAGCAGTTCA GCAGAGTCTG GAGCAGGAGG AGGCTGAACA TAAAACCACT 
AAAGCCAGAT TGGCCGACAA CAACAAAATC AACCAATCCA TAGAGGCAAA GTCTGAGACC 
CTGAAAGACA TGGAACATAA GCTGTTGGAA GAACGATCTG CGAAGCAACA ACTGGAAAAC 
AGACTGATGC AGCTGGAGAA GGAAAACTCT GTATTAGACT GTGATTACAA ACAAGCCAAA 
CATGAGCTTC AGGAGCTCCG CTCGCTCAAA GAAAATCTCA CAGAGCAGGT GGAGGTGCTG 
AATGTGCGGG TGCAGCAGGA GACACAGAGG AAAACTCTCT GTCAAGGTGA TCTCAAAGTT 
CAGAGGCAGG AGATCAACAG CCTGCGGTCA TCAGAACAAC AGCTCAAACA AGAACTCAAC 
CATCTGCTGG AGCTCAAACT CACACTGGAG AAACAAAACC AGGAGCTGTC CAAGGAACGA 
GAAGAATCTG AAAAACAGCT GAAGGAGATG AAGGACCAGC TGGAGGCTGA GCAGTACTTC 
ACGAAACTGT ACAAGACTCA GATTCGAGAG CTGAAAGAGG AAAGCGATGA AAAGGTTAAA 
CTCTACAAAG ATGCTCAGCA GAGAATAGAG GACCTGCAGG AAGAGAGGGA CTCGTTAGCG 
TCTCAGTTGG AGGTGAGCCT GACGAAGGCT GACTCTGAAC AGTTGGCTCG CATCACCGTT 
GAAGAGCAAT ACTCGGACCT GGAGAAAGAG AAGATCATGA AGGAACTGGA GATAAAGGAC 
ATGATCGCCA GACACCGGCA GGATCTTGCA GAAAAGGACG GTACCATCAA CTCGCTGGAG 
GAGTCAAACC GTACGCTGAC TGTGGATGTT GCCAACCTGG CCAGTGAGAA AGAGGAGCTC 
AACAATAAAC TGAAGCACAT ACAGCAAAAG CTGGAGAAGA TCAAAGAAGA AGAGAAACAG 
ATGAAATCTC TCACAGTGTC GTATGAGAAG CAGATACAGG TGGAGAAAAC ACTCAAGATT 
CAGGCTATAA ACAAGCTGGC GGAGGTCATG AAGAAGACGG ACGGCAGGCC ACACCAAATC 
AACAACACAG ACATCCGCAG GAAGGAGAAG GAGATCAGAC AACTCCAGCT GCAGCTCCGC 
ACGGAGAAAG AAAAGCTTAA CCACTCCATC ATCAAATACC AGAGAGAAAT CAACGACATG 
CAGGCTATGA TAGCAGAGGA GTCTCAGGTG CGTCTGGAGA TGCAGATGTC TCTGGACAGT 
AAAGACAGTG ATATTGAGCG TCTGCGCAGT CAACTCACGT CTCTGAGCAT TCACTCTCTG 
GACACCACCA GCATCAGCAG CATCGGCAAT GACCTGGATT CTGACGAGGC ATATCCAGCC 
GCAGAGTCCG GTCTTGAAGG ATGGCTCTCA ATGCCTGCCA AAAACACCAA ACGGTTTGGA 
TGGGATAAGA AGTATGTGGT TGTGAGCAGC AAGAAAATCC TGTTTTATGA CTCTGAACAA 
GACCGAGAGC AGTCCAGTCC ATTCATGATC CTGGACATTG ACAAACTCTT CCACGTGAGA 
CCCGTCACTC AGACAGATGT TTACCGCGCA GATCCCAGAG AAATCCCTCG CATCTTCCAG 
ATACTATATG CGAATGAAGG AGAGAGCAAG AAAGAGGAGC TGCCCGTGGA GTCGTTATCT 
GTGACTGAGA GGTCACTGTG TATCCCACAC AAGGGTCACG AGTTTGTTCT GACGCTTTAT 
CACTTCCCCT CGAGCTGCGA GGTCTGTCCA CGACCCTTAT GGCACGTCTT CAGACCTCCG 
CCGGCTCTCG AGTGCAGACG CTGTAGAGTC AAGTGCCATA AAGACCACAT CGATCGCAAA 
GAAGAGGTCC TCGCTCCATG TAGAGTGAAT TACGACATGT CCACAGCGAA AGAGATGCTG 
CTGCTGGCAA ACTCCACAGA GGAGCAGAAG AAGTGGGTCA GTCGGCTGCT CAAGAGGGTT 
CCGCGTAAAC CTTCCATCGC CCACTCTTCC AGCATAGCCA TATCCGCTGC GCCCTCAGAG 
GCAACACCCA CTACCCTTTC CCCTCAGCCG TCGCCTCGCC TCGCTCAGTC TTCACCCAGA 
CTTTCACACC GAGCAGCCGT CAAAGTGCAC TCTACACGAC AGCAGCCCTC CAGCAAGAAC 
AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rock2b ( XM_009294695.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009292970.1 Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X8, mRNA.

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CloneID	ODa24918
Clone ID Related Accession (Same CDS sequence)	XM_009294695.2 , XM_009294695.3
Accession Version	XM_009294695.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho-associated, coiled-coil containing protein kinase 2b isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009294695.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGACCG GAGCGGGGAA GAGAATAGAG AGCCGGGTGA GAAAACTAGA GACGCTGATC 
AAGAACCAGA AATCACCGTT GAACTTGGAG AGTTTGCTGG ATTCAGTAAC TGCCCTTGCC 
CTTGATCTGA ACCATCCAGC ACTGAGAAAG AACAAAAACA TAGATGCCTT CTTAAGCAGA 
TATGAAAAAG CCGTGTGCAA CCTCAAGGAA CTACAGGTGA AGCTGGATGA TTTTGATCGA 
GTCAAGCTGA TCGGTCGTGG AGCGTTTGGC GCAGTGCAGC TGGTACGGCA CAAAGTATCT 
CAACAAGTTT ATGCCATGAA GCAGCTCAGC AAGTTTGAGA TGGTCAAGCG CTCTGACTCC 
GCCTTTTTCT GGGAGGAGCG TGACATCATG GCTTTCTCCC AGAGCCCTTG GATAGTTCAG 
CTGTGCTGTG CATTCCAAGA TGAAAAGTAC CTTTATTTGG TGATGGAGTT CATGCCAGGT 
GGAGATCTGG TGACTCTGAC CAGCAACTAT GACATCCCAG AGGAATGGGC TCAATTCTAT 
ACGGCAGAAG TGGTGCTGGC ACTCGACGCC ATCCATTCTC TGGGCTTCAT CCACAGAGAC 
ATCAAACCAG ACAACATGCT GCTGGACCGC AATGGACACC TCAAACTCGC AGACTTTGGC 
ACATGCATGA AGATGGACTC GACTGGTATG GTACGCTGTG ACACTGCTGT TGGGACACCT 
GATTACATTT CTCCAGAGGT TTTAATGTCT CAAGGAGGGA CTGGTTATTA CGGTCGCGAG 
TGTGACTGGT GGTCTGTTGG TGTTTTTATT TATGAGCTTC TAGTGGGCGA CACTCCATTT 
TATTCCGAGT CTCTGGTGGG CACATATGGA AAGATCATGG ATCACAAGAA CAGCTTAACT 
TTCCCAGATG ATATTGAAAT GTCAAAGAAT GCCAAAGACC TCATCTGTGC CTTTCTGAGT 
AGCAGGGAGG TGAGGCTTGG CCGAACTGGA GTGGATGAAA TAAAATGTCA CCCCTTCTTT 
AAGAATGATC AGTGGACCTT CGACACCATA CGAGACACCA TGGCTCCAGT GGTGCCAGAG 
CTGAGTAGCG ATATTGACAC CAGTAACTTT GATGATGACA TTAAGGATGA TCCGATAGGA 
ACAGAGACGT TCCCTCCGCC CCGTGCCTTC GCAGGGAACC AGCTACCATT TGTGGGTTTC 
ACCTATTTCA GGGAGGACCA ATTGTTAACA CAACAAAATA AGTGTTCAGA GGAAGATATC 
ATTGAAGATC ATGGCTTAAA CCACACAGAG TCTAACGACC TGGAGAAGAG GCTGAAAAAG 
TTGGAGGAAA AATTCAAACA TGAGATGCAA GCAAAGGAAG AACTGGAAAA TAAATGCCGA 
ATAGCAAATC AGCGGCTGGA GAAATTGTCC AAAGATCTTG AAGAAGAAGT GAATGCGAGA 
CAGGAGGCGG AAGACAACCT GAGATCCCTG GAGAAAGAGA AAGTTTTACT GAAACACCAG 
CGTACACAGA GCGTAAGAAA AGCTGGTCTA GAGACCGACA GAAAACGGCT GCTGGAGAAT 
GAAGTAAGCA GCCTGAAAGA GCAGCTTGCC GAACTGAAGA AAAAGAACCA GATCTCTCAT 
CTGTCTGCTG AGAAGAACAT TCACCTGGAA AGACAGCTGG AGGAGGTGAG TGCCAAGCTT 
CAGGCCGAGC TGGAGGAGTC GGAGAGGCTG AAGAAGGCCC AGATCGAGGC CTTTAGACAG 
TCCCAGCAGC TGGAGCTTTC CCTCAGGGAG CTGCAGGAGC GTCTGGCCCA GCTGGAGAAC 
AGCAGGCTGG TTCTGGAGCA GGACAAACTC AGCCTGCAGA CCTCGCTAGA GCTGGAGAAA 
CGAGAGAGAA ACGCCGGCTC AGAGACCATC ACTGACCTGC AGGGTCGTAT CTATGGACTG 
GAGGGTGAGT TGAAGCATAT AAAGAGTTCT CTATCAAAAG CTGAAGTTGA GAAAAGACAA 
TTACAAGAGG ATCTTACAAC CCTGGAAAAG GAGAAGAATA AGCAGGAGAT CGACCTCTCC 
TTCAAACTCA AAGCAGTTCA GCAGAGTCTG GAGCAGGAGG AGGCTGAACA TAAAACCACT 
AAAGCCAGAT TGGCCGACAA CAACAAAATC AACCAATCCA TAGAGGCAAA GTCTGAGACC 
CTGAAAGACA TGGAACATAA GCTGTTGGAA GAACGATCTG CGAAGCAACA ACTGGAAAAC 
AGACTGATGC AGCTGGAGAA GGAAAACTCT GTATTAGACT GTGATTACAA ACAAGCCAAA 
CATGAGCTTC AGGAGCTCCG CTCGCTCAAA GAAAATCTCA CAGAGCAGGT GGAGGTGCTG 
AATGTGCGGG TGCAGCAGGA GACACAGAGG AAAACTCTCT GTCAAGGTGA TCTCAAAGTT 
CAGAGGCAGG AGATCAACAG CCTGCGGTCA TCAGAACAAC AGCTCAAACA AGAACTCAAC 
CATCTGCTGG AGCTCAAACT CACACTGGAG AAACAAAACC AGGAGCTGTC CAAGGAACGA 
GAAGAATCTG AAAAACAGCT GAAGGAGATG AAGGACCAGC TGGAGGCTGA GCAGTACTTC 
ACGAAACTGT ACAAGACTCA GATTCGAGAG CTGAAAGAGG AAAGCGATGA AAAGGTTAAA 
CTCTACAAAG ATGCTCAGCA GAGAATAGAG GACCTGCAGG AAGAGAGGGA CTCGTTAGCG 
TCTCAGTTGG AGGTGAGCCT GACGAAGGCT GACTCTGAAC AGTTGGCTCG CATCACCGTT 
GAAGAGCAAT ACTCGGACCT GGAGAAAGAG AAGATCATGA AGGAACTGGA GATAAAGGAC 
ATGATCGCCA GACACCGGCA GGATCTTGCA GAAAAGGACG GTACCATCAA CTCGCTGGAG 
GAGTCAAACC GTACGCTGAC TGTGGATGTT GCCAACCTGG CCAGTGAGAA AGAGGAGCTC 
AACAATAAAC TGAAGCACAT ACAGCAAAAG CTGGAGAAGA TCAAAGAAGA AGAGAAACAG 
ATGAAATCTC TCACAGTGTC GTATGAGAAG CAGATACAGG TGGAGAAAAC ACTCAAGATT 
CAGGCTATAA ACAAGCTGGC GGAGGTCATG AAGAAGACGG ACGGCAGGCC ACACCAAATC 
AACAACACAG ACATCCGCAG GAAGGAGAAG GAGATCAGAC AACTCCAGCT GCAGCTCCGC 
ACGGAGAAAG AAAAGCTTAA CCACTCCATC ATCAAATACC AGAGAGAAAT CAACGACATG 
CAGGCTATGA TAGCAGAGGA GTCTCAGGTG CGTCTGGAGA TGCAGATGTC TCTGGACAGT 
AAAGACAGTG ATATTGAGCG TCTGCGCAGT CAACTCACGT CTCTGAGCAT TCACTCTCTG 
GACACCACCA GCATCAGCAG CATCGGCAAT GACCTGGATT CTGACGAGGC ATATCCAGCC 
GCAGAGTCCG GTCTTGAAGG ATGGCTCTCA ATGCCTGCCA AAAACACCAA ACGGTTTGGA 
TGGGATAAGA AGTATGTGGT TGTGAGCAGC AAGAAAATCC TGTTTTATGA CTCTGAACAA 
GACCGAGAGC AGTCCAGTCC ATTCATGATC CTGGACATTG ACAAACTCTT CCACGTGAGA 
CCCGTCACTC AGACAGATGT TTACCGCGCA GATCCCAGAG AAATCCCTCG CATCTTCCAG 
ATACTATATG CGAATGAAGG AGAGAGCAAG AAAGAGGAGC TGCCCGTGGA GTCGTTATCT 
GTGACTGAGA GGTCACTGTG TATCCCACAC AAGGGTCACG AGTTTGTTCT GACGCTTTAT 
CACTTCCCCT CGAGCTGCGA GGTCTGTCCA CGACCCTTAT GGCACGTCTT CAGACCTCCG 
CCGGCTCTCG AGTGCAGACG CTGTAGAGTC AAGTGCCATA AAGACCACAT CGATCGCAAA 
GAAGAGGTCC TCGCTCCATG TAGAGTGAAT TACGACATGT CCACAGCGAA AGAGATGCTG 
CTGCTGGCAA ACTCCACAGA GGAGCAGAAG AAGTGGGTCA GTCGGCTGCT CAAGAGGGTT 
CCGCGTAAAC CTTCCATCGC CCACTCTTCC AGCATAGCCA TATCCGCTGC GCCCTCAGAG 
GCAACACCCA CTACCCTTTC CCCTCAGCCG TCGCCTCGCC TCGCTCAGTC TTCACCCAGA 
CTTTCACACC GAGCAGCCGT CAAAGTGCAC TCTACACGAC AGCAGCCCTC CAGCAAGAAC 
AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009292970.1
CDS	473..4678
Translation	MSTGAGKRIESRVRKLETLIKNQKSPLNLESLLDSVTALALDLNHPALRKNKNIDAFLSRYEKAVCNLKELQVKLDDFDRVKLIGRGAFGAVQLVRHKVSQQVYAMKQLSKFEMVKRSDSAFFWEERDIMAFSQSPWIVQLCCAFQDEKYLYLVMEFMPGGDLVTLTSNYDIPEEWAQFYTAEVVLALDAIHSLGFIHRDIKPDNMLLDRNGHLKLADFGTCMKMDSTGMVRCDTAVGTPDYISPEVLMSQGGTGYYGRECDWWSVGVFIYELLVGDTPFYSESLVGTYGKIMDHKNSLTFPDDIEMSKNAKDLICAFLSSREVRLGRTGVDEIKCHPFFKNDQWTFDTIRDTMAPVVPELSSDIDTSNFDDDIKDDPIGTETFPPPRAFAGNQLPFVGFTYFREDQLLTQQNKCSEEDIIEDHGLNHTESNDLEKRLKKLEEKFKHEMQAKEELENKCRIANQRLEKLSKDLEEEVNARQEAEDNLRSLEKEKVLLKHQRTQSVRKAGLETDRKRLLENEVSSLKEQLAELKKKNQISHLSAEKNIHLERQLEEVSAKLQAELEESERLKKAQIEAFRQSQQLELSLRELQERLAQLENSRLVLEQDKLSLQTSLELEKRERNAGSETITDLQGRIYGLEGELKHIKSSLSKAEVEKRQLQEDLTTLEKEKNKQEIDLSFKLKAVQQSLEQEEAEHKTTKARLADNNKINQSIEAKSETLKDMEHKLLEERSAKQQLENRLMQLEKENSVLDCDYKQAKHELQELRSLKENLTEQVEVLNVRVQQETQRKTLCQGDLKVQRQEINSLRSSEQQLKQELNHLLELKLTLEKQNQELSKEREESEKQLKEMKDQLEAEQYFTKLYKTQIRELKEESDEKVKLYKDAQQRIEDLQEERDSLASQLEVSLTKADSEQLARITVEEQYSDLEKEKIMKELEIKDMIARHRQDLAEKDGTINSLEESNRTLTVDVANLASEKEELNNKLKHIQQKLEKIKEEEKQMKSLTVSYEKQIQVEKTLKIQAINKLAEVMKKTDGRPHQINNTDIRRKEKEIRQLQLQLRTEKEKLNHSIIKYQREINDMQAMIAEESQVRLEMQMSLDSKDSDIERLRSQLTSLSIHSLDTTSISSIGNDLDSDEAYPAAESGLEGWLSMPAKNTKRFGWDKKYVVVSSKKILFYDSEQDREQSSPFMILDIDKLFHVRPVTQTDVYRADPREIPRIFQILYANEGESKKEELPVESLSVTERSLCIPHKGHEFVLTLYHFPSSCEVCPRPLWHVFRPPPALECRRCRVKCHKDHIDRKEEVLAPCRVNYDMSTAKEMLLLANSTEEQKKWVSRLLKRVPRKPSIAHSSSIAISAAPSEATPTTLSPQPSPRLAQSSPRLSHRAAVKVHSTRQQPSSKNR

Target ORF information:

RefSeq Version	XM_009294695.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009294695.3

ORF Insert Sequence:

ATGTCGACCG GAGCGGGGAA GAGAATAGAG AGCCGGGTGA GAAAACTAGA GACGCTGATC 
AAGAACCAGA AATCACCGTT GAACTTGGAG AGTTTGCTGG ATTCAGTAAC TGCCCTTGCC 
CTTGATCTGA ACCATCCAGC ACTGAGAAAG AACAAAAACA TAGATGCCTT CTTAAGCAGA 
TATGAAAAAG CCGTGTGCAA CCTCAAGGAA CTACAGGTGA AGCTGGATGA TTTTGATCGA 
GTCAAGCTGA TCGGTCGTGG AGCGTTTGGC GCAGTGCAGC TGGTACGGCA CAAAGTATCT 
CAACAAGTTT ATGCCATGAA GCAGCTCAGC AAGTTTGAGA TGGTCAAGCG CTCTGACTCC 
GCCTTTTTCT GGGAGGAGCG TGACATCATG GCTTTCTCCC AGAGCCCTTG GATAGTTCAG 
CTGTGCTGTG CATTCCAAGA TGAAAAGTAC CTTTATTTGG TGATGGAGTT CATGCCAGGT 
GGAGATCTGG TGACTCTGAC CAGCAACTAT GACATCCCAG AGGAATGGGC TCAATTCTAT 
ACGGCAGAAG TGGTGCTGGC ACTCGACGCC ATCCATTCTC TGGGCTTCAT CCACAGAGAC 
ATCAAACCAG ACAACATGCT GCTGGACCGC AATGGACACC TCAAACTCGC AGACTTTGGC 
ACATGCATGA AGATGGACTC GACTGGTATG GTACGCTGTG ACACTGCTGT TGGGACACCT 
GATTACATTT CTCCAGAGGT TTTAATGTCT CAAGGAGGGA CTGGTTATTA CGGTCGCGAG 
TGTGACTGGT GGTCTGTTGG TGTTTTTATT TATGAGCTTC TAGTGGGCGA CACTCCATTT 
TATTCCGAGT CTCTGGTGGG CACATATGGA AAGATCATGG ATCACAAGAA CAGCTTAACT 
TTCCCAGATG ATATTGAAAT GTCAAAGAAT GCCAAAGACC TCATCTGTGC CTTTCTGAGT 
AGCAGGGAGG TGAGGCTTGG CCGAACTGGA GTGGATGAAA TAAAATGTCA CCCCTTCTTT 
AAGAATGATC AGTGGACCTT CGACACCATA CGAGACACCA TGGCTCCAGT GGTGCCAGAG 
CTGAGTAGCG ATATTGACAC CAGTAACTTT GATGATGACA TTAAGGATGA TCCGATAGGA 
ACAGAGACGT TCCCTCCGCC CCGTGCCTTC GCAGGGAACC AGCTACCATT TGTGGGTTTC 
ACCTATTTCA GGGAGGACCA ATTGTTAACA CAACAAAATA AGTGTTCAGA GGAAGATATC 
ATTGAAGATC ATGGCTTAAA CCACACAGAG TCTAACGACC TGGAGAAGAG GCTGAAAAAG 
TTGGAGGAAA AATTCAAACA TGAGATGCAA GCAAAGGAAG AACTGGAAAA TAAATGCCGA 
ATAGCAAATC AGCGGCTGGA GAAATTGTCC AAAGATCTTG AAGAAGAAGT GAATGCGAGA 
CAGGAGGCGG AAGACAACCT GAGATCCCTG GAGAAAGAGA AAGTTTTACT GAAACACCAG 
CGTACACAGA GCGTAAGAAA AGCTGGTCTA GAGACCGACA GAAAACGGCT GCTGGAGAAT 
GAAGTAAGCA GCCTGAAAGA GCAGCTTGCC GAACTGAAGA AAAAGAACCA GATCTCTCAT 
CTGTCTGCTG AGAAGAACAT TCACCTGGAA AGACAGCTGG AGGAGGTGAG TGCCAAGCTT 
CAGGCCGAGC TGGAGGAGTC GGAGAGGCTG AAGAAGGCCC AGATCGAGGC CTTTAGACAG 
TCCCAGCAGC TGGAGCTTTC CCTCAGGGAG CTGCAGGAGC GTCTGGCCCA GCTGGAGAAC 
AGCAGGCTGG TTCTGGAGCA GGACAAACTC AGCCTGCAGA CCTCGCTAGA GCTGGAGAAA 
CGAGAGAGAA ACGCCGGCTC AGAGACCATC ACTGACCTGC AGGGTCGTAT CTATGGACTG 
GAGGGTGAGT TGAAGCATAT AAAGAGTTCT CTATCAAAAG CTGAAGTTGA GAAAAGACAA 
TTACAAGAGG ATCTTACAAC CCTGGAAAAG GAGAAGAATA AGCAGGAGAT CGACCTCTCC 
TTCAAACTCA AAGCAGTTCA GCAGAGTCTG GAGCAGGAGG AGGCTGAACA TAAAACCACT 
AAAGCCAGAT TGGCCGACAA CAACAAAATC AACCAATCCA TAGAGGCAAA GTCTGAGACC 
CTGAAAGACA TGGAACATAA GCTGTTGGAA GAACGATCTG CGAAGCAACA ACTGGAAAAC 
AGACTGATGC AGCTGGAGAA GGAAAACTCT GTATTAGACT GTGATTACAA ACAAGCCAAA 
CATGAGCTTC AGGAGCTCCG CTCGCTCAAA GAAAATCTCA CAGAGCAGGT GGAGGTGCTG 
AATGTGCGGG TGCAGCAGGA GACACAGAGG AAAACTCTCT GTCAAGGTGA TCTCAAAGTT 
CAGAGGCAGG AGATCAACAG CCTGCGGTCA TCAGAACAAC AGCTCAAACA AGAACTCAAC 
CATCTGCTGG AGCTCAAACT CACACTGGAG AAACAAAACC AGGAGCTGTC CAAGGAACGA 
GAAGAATCTG AAAAACAGCT GAAGGAGATG AAGGACCAGC TGGAGGCTGA GCAGTACTTC 
ACGAAACTGT ACAAGACTCA GATTCGAGAG CTGAAAGAGG AAAGCGATGA AAAGGTTAAA 
CTCTACAAAG ATGCTCAGCA GAGAATAGAG GACCTGCAGG AAGAGAGGGA CTCGTTAGCG 
TCTCAGTTGG AGGTGAGCCT GACGAAGGCT GACTCTGAAC AGTTGGCTCG CATCACCGTT 
GAAGAGCAAT ACTCGGACCT GGAGAAAGAG AAGATCATGA AGGAACTGGA GATAAAGGAC 
ATGATCGCCA GACACCGGCA GGATCTTGCA GAAAAGGACG GTACCATCAA CTCGCTGGAG 
GAGTCAAACC GTACGCTGAC TGTGGATGTT GCCAACCTGG CCAGTGAGAA AGAGGAGCTC 
AACAATAAAC TGAAGCACAT ACAGCAAAAG CTGGAGAAGA TCAAAGAAGA AGAGAAACAG 
ATGAAATCTC TCACAGTGTC GTATGAGAAG CAGATACAGG TGGAGAAAAC ACTCAAGATT 
CAGGCTATAA ACAAGCTGGC GGAGGTCATG AAGAAGACGG ACGGCAGGCC ACACCAAATC 
AACAACACAG ACATCCGCAG GAAGGAGAAG GAGATCAGAC AACTCCAGCT GCAGCTCCGC 
ACGGAGAAAG AAAAGCTTAA CCACTCCATC ATCAAATACC AGAGAGAAAT CAACGACATG 
CAGGCTATGA TAGCAGAGGA GTCTCAGGTG CGTCTGGAGA TGCAGATGTC TCTGGACAGT 
AAAGACAGTG ATATTGAGCG TCTGCGCAGT CAACTCACGT CTCTGAGCAT TCACTCTCTG 
GACACCACCA GCATCAGCAG CATCGGCAAT GACCTGGATT CTGACGAGGC ATATCCAGCC 
GCAGAGTCCG GTCTTGAAGG ATGGCTCTCA ATGCCTGCCA AAAACACCAA ACGGTTTGGA 
TGGGATAAGA AGTATGTGGT TGTGAGCAGC AAGAAAATCC TGTTTTATGA CTCTGAACAA 
GACCGAGAGC AGTCCAGTCC ATTCATGATC CTGGACATTG ACAAACTCTT CCACGTGAGA 
CCCGTCACTC AGACAGATGT TTACCGCGCA GATCCCAGAG AAATCCCTCG CATCTTCCAG 
ATACTATATG CGAATGAAGG AGAGAGCAAG AAAGAGGAGC TGCCCGTGGA GTCGTTATCT 
GTGACTGAGA GGTCACTGTG TATCCCACAC AAGGGTCACG AGTTTGTTCT GACGCTTTAT 
CACTTCCCCT CGAGCTGCGA GGTCTGTCCA CGACCCTTAT GGCACGTCTT CAGACCTCCG 
CCGGCTCTCG AGTGCAGACG CTGTAGAGTC AAGTGCCATA AAGACCACAT CGATCGCAAA 
GAAGAGGTCC TCGCTCCATG TAGAGTGAAT TACGACATGT CCACAGCGAA AGAGATGCTG 
CTGCTGGCAA ACTCCACAGA GGAGCAGAAG AAGTGGGTCA GTCGGCTGCT CAAGAGGGTT 
CCGCGTAAAC CTTCCATCGC CCACTCTTCC AGCATAGCCA TATCCGCTGC GCCCTCAGAG 
GCAACACCCA CTACCCTTTC CCCTCAGCCG TCGCCTCGCC TCGCTCAGTC TTCACCCAGA 
CTTTCACACC GAGCAGCCGT CAAAGTGCAC TCTACACGAC AGCAGCCCTC CAGCAAGAAC 
AGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rock2b ( XM_017352432.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207921.1 Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa39639
Clone ID Related Accession (Same CDS sequence)	XM_017352432.1 , XM_021468356.1
Accession Version	XM_017352432.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	rho-associated, coiled-coil containing protein kinase 2b isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGACCG GAGCGGGGAA GAGAATAGAG AGCCGGGTGA GAAAACTAGA GACGCTGATC 
AAGAACCAGA AATCACCGTT GAACTTGGAG AGTTTGCTGG ATTCAGTAAC TGCCCTTGCC 
CTTGATCTGA ACCATCCAGC ACTGAGAAAG AACAAAAACA TAGATGCCTT CTTAAGCAGA 
TATGAAAAAG CCGTGTGCAA CCTCAAGGAA CTACAGGTGA AGCTGGATGA TTTTGATCGA 
GTCAAGCTGA TCGGTCGTGG AGCGTTTGGC GCAGTGCAGC TGGTACGGCA CAAAGTATCT 
CAACAAGTTT ATGCCATGAA GCAGCTCAGC AAGTTTGAGA TGGTCAAGCG CTCTGACTCC 
GCCTTTTTCT GGGAGGAGCG TGACATCATG GCTTTCTCCC AGAGCCCTTG GATAGTTCAG 
CTGTGCTGTG CATTCCAAGA TGAAAAGTAC CTTTATTTGG TGATGGAGTT CATGCCAGGT 
GGAGATCTGG TGACTCTGAC CAGCAACTAT GACATCCCAG AGGAATGGGC TCAATTCTAT 
ACGGCAGAAG TGGTGCTGGC ACTCGACGCC ATCCATTCTC TGGGCTTCAT CCACAGAGAC 
ATCAAACCAG ACAACATGCT GCTGGACCGC AATGGACACC TCAAACTCGC AGACTTTGGC 
ACATGCATGA AGATGGACTC GACTGGTATG GTACGCTGTG ACACTGCTGT TGGGACACCT 
GATTACATTT CTCCAGAGGT TTTAATGTCT CAAGGAGGGA CTGGTTATTA CGGTCGCGAG 
TGTGACTGGT GGTCTGTTGG TGTTTTTATT TATGAGCTTC TAGTGGGCGA CACTCCATTT 
TATTCCGAGT CTCTGGTGGG CACATATGGA AAGATCATGG ATCACAAGAA CAGCTTAACT 
TTCCCAGATG ATATTGAAAT GTCAAAGAAT GCCAAAGACC TCATCTGTGC CTTTCTGAGT 
AGCAGGGAGG TGAGGCTTGG CCGAACTGGA GTGGATGAAA TAAAATGTCA CCCCTTCTTT 
AAGAATGATC AGTGGACCTT CGACACCATA CGAGACACCA TGGCTCCAGT GGTGCCAGAG 
CTGAGTAGCG ATATTGACAC CAGTAACTTT GATGATGACA TTAAGGATGA TCCGATAGGA 
ACAGAGACGT TCCCTCCGCC CCGTGCCTTC GCAGGGAACC AGCTACCATT TGTGGGTTTC 
ACCTATTTCA GGGAGGACCA ATTGTTAACA CAACAAAATA AGTGTTCAGA GGAAGATATC 
ATTGAAGATC ATGGCTTAAA CCACACAGAG TCTAACGACC TGGAGAAGAG GCTGAAAAAG 
TTGGAGGAAA AATTCAAACA TGAGATGCAA GCAAAGGAAG AACTGGAAAA TAAATGCCGA 
ATAGCAAATC AGCGGCTGGA GAAATTGTCC AAAGATCTTG AAGAAGAAGT GAATGCGAGA 
CAGGAGGCGG AAGACAACCT GAGATCCCTG GAGAAAGAGA AAGTTTTACT GAAACACCAG 
CGTACACAGA GCGTAAGAAA AGCTGGTCTA GAGACCGACA GAAAACGGCT GCTGGAGAAT 
GAAGTAAGCA GCCTGAAAGA GCAGCTTGCC GAACTGAAGA AAAAGAACCA GATCTCTCAT 
CTGTCTGCTG AGAAGAACAT TCACCTGGAA AGACAGCTGG AGGAGGTGAG TGCCAAGCTT 
CAGGCCGAGC TGGAGGAGTC GGAGAGGCTG AAGAAGGCCC AGATCGAGGC CTTTAGACAG 
TCCCAGCAGC TGGAGCTTTC CCTCAGGGAG CTGCAGGAGC GTCTGGCCCA GCTGGAGAAC 
AGCAGGCTGG TTCTGGAGCA GGACAAACTC AGCCTGCAGA CCTCGCTAGA GCTGGAGAAA 
CGAGAGAGAA ACGCCGGCTC AGAGACCATC ACTGACCTGC AGGGTCGTAT CTATGGACTG 
GAGGGTGAGT TGAAGCATAT AAAGAGTTCT CTATCAAAAG CTGAAGTTGA GAAAAGACAA 
TTACAAGAGG ATCTTACAAC CCTGGAAAAG GAGAAGAATA AGCAGGAGAT CGACCTCTCC 
TTCAAACTCA AAGCAGTTCA GCAGAGTCTG GAGCAGGAGG AGGCTGAACA TAAAACCACT 
AAAGCCAGAT TGGCCGACAA CAACAAAATC AACCAATCCA TAGAGGCAAA GTCTGAGACC 
CTGAAAGACA TGGAACATAA GCTGTTGGAA GAACGATCTG CGAAGCAACA ACTGGAAAAC 
AGACTGATGC AGCTGGAGAA GGAAAACTCT GTATTAGACT GTGATTACAA ACAAGCCAAA 
CATGAGCTTC AGGAGCTCCG CTCGCTCAAA GAAAATCTCA CAGAGCAGGT GGAGGTGCTG 
AATGTGCGGG TGCAGCAGGA GACACAGAGG AAAACTCTCT GTCAAGGTGA TCTCAAAGTT 
CAGAGGCAGG AGATCAACAG CCTGCGGTCA TCAGAACAAC AGCTCAAACA AGAACTCAAC 
CATCTGCTGG AGCTCAAACT CACACTGGAG AAACAAAACC AGGAGCTGTC CAAGGAACGA 
GAAGAATCTG AAAAACAGCT GAAGGAGATG AAGGACCAGC TGGAGGCTGA GCAGTACTTC 
ACGAAACTGT ACAAGACTCA GATTCGAGAG CTGAAAGAGG AAAGCGATGA AAAGGTTAAA 
CTCTACAAAG ATGCTCAGCA GAGAATAGAG GACCTGCAGG AAGAGAGGGA CTCGTTAGCG 
TCTCAGTTGG AGGTGAGCCT GACGAAGGCT GACTCTGAAC AGTTGGCTCG CATCACCGTT 
GAAGAGCAAT ACTCGGACCT GGAGAAAGAG AAGATCATGA AGGAACTGGA GATAAAGGAC 
ATGATCGCCA GACACCGGCA GGATCTTGCA GAAAAGGACG GTACCATCAA CTCGCTGGAG 
GAGTCAAACC GTACGCTGAC TGTGGATGTT GCCAACCTGG CCAGTGAGAA AGAGGAGCTC 
AACAATAAAC TGAAGCACAT ACAGCAAAAG CTGGAGAAGA TCAAAGAAGA AGAGAAACAG 
ATGAAATCTC TCACAGTGTC GTATGAGAAG CAGATACAGG TGGAGAAAAC ACTCAAGATT 
CAGGCTATAA ACAAGCTGGC GGAGGTCATG AAGAAGACGG ACGGCAGGCC ACACCAAATC 
AACAACACAG ACATCCGCAG GAAGGAGAAG GAGATCAGAC AACTCCAGCT GCAGCTCCGC 
ACGGAGAAAG AAAAGCTTAA CCACTCCATC ATCAAATACC AGAGAGAAAT CAACGACATG 
CAGGCTATGA TAGCAGAGGA GTCTCAGGTG CGTCTGGAGA TGCAGATGTC TCTGGACAGT 
AAAGACAGTG ATATTGAGCG TCTGCGCAGT CAACTCACGT CTCTGAGCAT TCACTCTCTG 
GACACCACCA GCATCAGCAG CATCGGCAAT GACCTGGATT CTGACGAGGC ATATCCAGTG 
CGAATCACAC ACTCACACAC CTCGGAGTCT ATGTCCTTCA CATACCAGCG AAGCTCCAAG 
TCGGTGTGTA TTGATACCCG GCCCTGTCGA ATCGCTCCTC GCTTCTCCTC TGACTCTGAT 
GAAGAGGAGG ATGAGGAAGA TGAGTGGAGA CAAACACCTG CCCTTACATA TGAGACGCCT 
CCTGACCCTG AGGCTGCAGC CGCAGAGTCC GGTCTTGAAG GATGGCTCTC AATGCCTGCC 
AAAAACACCA AACGGTTTGG ATGGGATAAG AAGTATGTGG TTGTGAGCAG CAAGAAAATC 
CTGTTTTATG ACTCTGAACA AGACCGAGAG CAGTCCAGTC CATTCATGAT CCTGGACATT 
GACAAACTCT TCCACGTGAG ACCCGTCACT CAGACAGATG TTTACCGCGC AGATCCCAGA 
GAAATCCCTC GCATCTTCCA GATACTATAT GCGAATGAAG GAGAGAGCAA GAAAGAGGAG 
CTGCCCGTGG AGTCGTTATC TGTGACTGAG AGGTCACTGT GTATCCCACA CAAGGGTCAC 
GAGTTTGTTC TGACGCTTTA TCACTTCCCC TCGAGCTGCG AGGTCTGTCC ACGACCCTTA 
TGGCACGTCT TCAGACCTCC GCCGGCTCTC GAGTGCAGAC GCTGTAGAGT CAAGTGCCAT 
AAAGACCACA TCGATCGCAA AGAAGAGGTC CTCGCTCCAT GTAGAGTGAA TTACGACATG 
TCCACAGCGA AAGAGATGCT GCTGCTGGCA AACTCCACAG AGGAGCAGAA GAAGTGGGTC 
AGTCGGCTGC TCAAGAGGGT TCCGCGTAAA CCTTCCATCG CCCACTCTTC CAGCATAGCC 
ATATCCGCTG CGCCCTCAGA GGCAACACCC ACTACCCTTT CCCCTCAGCC GTCGCCTCGC 
CTCGCTCAGT CTTCACCCAG ACTTTCACAC CGAGCAGCCG TCAAAGTGCA CTCTACACGA 
CAGCAGCCCT CCAGCAAGAA CAGATTGCTT GAAATGGGCC TGAAGGACTG GGGTTGGGCT 
CTTGAGGTTG AATTGGATGA TGACGATGTT TTAGACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207921.1
CDS	589..5070
Translation	MSTGAGKRIESRVRKLETLIKNQKSPLNLESLLDSVTALALDLNHPALRKNKNIDAFLSRYEKAVCNLKELQVKLDDFDRVKLIGRGAFGAVQLVRHKVSQQVYAMKQLSKFEMVKRSDSAFFWEERDIMAFSQSPWIVQLCCAFQDEKYLYLVMEFMPGGDLVTLTSNYDIPEEWAQFYTAEVVLALDAIHSLGFIHRDIKPDNMLLDRNGHLKLADFGTCMKMDSTGMVRCDTAVGTPDYISPEVLMSQGGTGYYGRECDWWSVGVFIYELLVGDTPFYSESLVGTYGKIMDHKNSLTFPDDIEMSKNAKDLICAFLSSREVRLGRTGVDEIKCHPFFKNDQWTFDTIRDTMAPVVPELSSDIDTSNFDDDIKDDPIGTETFPPPRAFAGNQLPFVGFTYFREDQLLTQQNKCSEEDIIEDHGLNHTESNDLEKRLKKLEEKFKHEMQAKEELENKCRIANQRLEKLSKDLEEEVNARQEAEDNLRSLEKEKVLLKHQRTQSVRKAGLETDRKRLLENEVSSLKEQLAELKKKNQISHLSAEKNIHLERQLEEVSAKLQAELEESERLKKAQIEAFRQSQQLELSLRELQERLAQLENSRLVLEQDKLSLQTSLELEKRERNAGSETITDLQGRIYGLEGELKHIKSSLSKAEVEKRQLQEDLTTLEKEKNKQEIDLSFKLKAVQQSLEQEEAEHKTTKARLADNNKINQSIEAKSETLKDMEHKLLEERSAKQQLENRLMQLEKENSVLDCDYKQAKHELQELRSLKENLTEQVEVLNVRVQQETQRKTLCQGDLKVQRQEINSLRSSEQQLKQELNHLLELKLTLEKQNQELSKEREESEKQLKEMKDQLEAEQYFTKLYKTQIRELKEESDEKVKLYKDAQQRIEDLQEERDSLASQLEVSLTKADSEQLARITVEEQYSDLEKEKIMKELEIKDMIARHRQDLAEKDGTINSLEESNRTLTVDVANLASEKEELNNKLKHIQQKLEKIKEEEKQMKSLTVSYEKQIQVEKTLKIQAINKLAEVMKKTDGRPHQINNTDIRRKEKEIRQLQLQLRTEKEKLNHSIIKYQREINDMQAMIAEESQVRLEMQMSLDSKDSDIERLRSQLTSLSIHSLDTTSISSIGNDLDSDEAYPVRITHSHTSESMSFTYQRSSKSVCIDTRPCRIAPRFSSDSDEEEDEEDEWRQTPALTYETPPDPEAAAAESGLEGWLSMPAKNTKRFGWDKKYVVVSSKKILFYDSEQDREQSSPFMILDIDKLFHVRPVTQTDVYRADPREIPRIFQILYANEGESKKEELPVESLSVTERSLCIPHKGHEFVLTLYHFPSSCEVCPRPLWHVFRPPPALECRRCRVKCHKDHIDRKEEVLAPCRVNYDMSTAKEMLLLANSTEEQKKWVSRLLKRVPRKPSIAHSSSIAISAAPSEATPTTLSPQPSPRLAQSSPRLSHRAAVKVHSTRQQPSSKNRLLEMGLKDWGWALEVELDDDDVLDF

Target ORF information:

RefSeq Version	XM_017352432.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352432.1

ORF Insert Sequence:

ATGTCGACCG GAGCGGGGAA GAGAATAGAG AGCCGGGTGA GAAAACTAGA GACGCTGATC 
AAGAACCAGA AATCACCGTT GAACTTGGAG AGTTTGCTGG ATTCAGTAAC TGCCCTTGCC 
CTTGATCTGA ACCATCCAGC ACTGAGAAAG AACAAAAACA TAGATGCCTT CTTAAGCAGA 
TATGAAAAAG CCGTGTGCAA CCTCAAGGAA CTACAGGTGA AGCTGGATGA TTTTGATCGA 
GTCAAGCTGA TCGGTCGTGG AGCGTTTGGC GCAGTGCAGC TGGTACGGCA CAAAGTATCT 
CAACAAGTTT ATGCCATGAA GCAGCTCAGC AAGTTTGAGA TGGTCAAGCG CTCTGACTCC 
GCCTTTTTCT GGGAGGAGCG TGACATCATG GCTTTCTCCC AGAGCCCTTG GATAGTTCAG 
CTGTGCTGTG CATTCCAAGA TGAAAAGTAC CTTTATTTGG TGATGGAGTT CATGCCAGGT 
GGAGATCTGG TGACTCTGAC CAGCAACTAT GACATCCCAG AGGAATGGGC TCAATTCTAT 
ACGGCAGAAG TGGTGCTGGC ACTCGACGCC ATCCATTCTC TGGGCTTCAT CCACAGAGAC 
ATCAAACCAG ACAACATGCT GCTGGACCGC AATGGACACC TCAAACTCGC AGACTTTGGC 
ACATGCATGA AGATGGACTC GACTGGTATG GTACGCTGTG ACACTGCTGT TGGGACACCT 
GATTACATTT CTCCAGAGGT TTTAATGTCT CAAGGAGGGA CTGGTTATTA CGGTCGCGAG 
TGTGACTGGT GGTCTGTTGG TGTTTTTATT TATGAGCTTC TAGTGGGCGA CACTCCATTT 
TATTCCGAGT CTCTGGTGGG CACATATGGA AAGATCATGG ATCACAAGAA CAGCTTAACT 
TTCCCAGATG ATATTGAAAT GTCAAAGAAT GCCAAAGACC TCATCTGTGC CTTTCTGAGT 
AGCAGGGAGG TGAGGCTTGG CCGAACTGGA GTGGATGAAA TAAAATGTCA CCCCTTCTTT 
AAGAATGATC AGTGGACCTT CGACACCATA CGAGACACCA TGGCTCCAGT GGTGCCAGAG 
CTGAGTAGCG ATATTGACAC CAGTAACTTT GATGATGACA TTAAGGATGA TCCGATAGGA 
ACAGAGACGT TCCCTCCGCC CCGTGCCTTC GCAGGGAACC AGCTACCATT TGTGGGTTTC 
ACCTATTTCA GGGAGGACCA ATTGTTAACA CAACAAAATA AGTGTTCAGA GGAAGATATC 
ATTGAAGATC ATGGCTTAAA CCACACAGAG TCTAACGACC TGGAGAAGAG GCTGAAAAAG 
TTGGAGGAAA AATTCAAACA TGAGATGCAA GCAAAGGAAG AACTGGAAAA TAAATGCCGA 
ATAGCAAATC AGCGGCTGGA GAAATTGTCC AAAGATCTTG AAGAAGAAGT GAATGCGAGA 
CAGGAGGCGG AAGACAACCT GAGATCCCTG GAGAAAGAGA AAGTTTTACT GAAACACCAG 
CGTACACAGA GCGTAAGAAA AGCTGGTCTA GAGACCGACA GAAAACGGCT GCTGGAGAAT 
GAAGTAAGCA GCCTGAAAGA GCAGCTTGCC GAACTGAAGA AAAAGAACCA GATCTCTCAT 
CTGTCTGCTG AGAAGAACAT TCACCTGGAA AGACAGCTGG AGGAGGTGAG TGCCAAGCTT 
CAGGCCGAGC TGGAGGAGTC GGAGAGGCTG AAGAAGGCCC AGATCGAGGC CTTTAGACAG 
TCCCAGCAGC TGGAGCTTTC CCTCAGGGAG CTGCAGGAGC GTCTGGCCCA GCTGGAGAAC 
AGCAGGCTGG TTCTGGAGCA GGACAAACTC AGCCTGCAGA CCTCGCTAGA GCTGGAGAAA 
CGAGAGAGAA ACGCCGGCTC AGAGACCATC ACTGACCTGC AGGGTCGTAT CTATGGACTG 
GAGGGTGAGT TGAAGCATAT AAAGAGTTCT CTATCAAAAG CTGAAGTTGA GAAAAGACAA 
TTACAAGAGG ATCTTACAAC CCTGGAAAAG GAGAAGAATA AGCAGGAGAT CGACCTCTCC 
TTCAAACTCA AAGCAGTTCA GCAGAGTCTG GAGCAGGAGG AGGCTGAACA TAAAACCACT 
AAAGCCAGAT TGGCCGACAA CAACAAAATC AACCAATCCA TAGAGGCAAA GTCTGAGACC 
CTGAAAGACA TGGAACATAA GCTGTTGGAA GAACGATCTG CGAAGCAACA ACTGGAAAAC 
AGACTGATGC AGCTGGAGAA GGAAAACTCT GTATTAGACT GTGATTACAA ACAAGCCAAA 
CATGAGCTTC AGGAGCTCCG CTCGCTCAAA GAAAATCTCA CAGAGCAGGT GGAGGTGCTG 
AATGTGCGGG TGCAGCAGGA GACACAGAGG AAAACTCTCT GTCAAGGTGA TCTCAAAGTT 
CAGAGGCAGG AGATCAACAG CCTGCGGTCA TCAGAACAAC AGCTCAAACA AGAACTCAAC 
CATCTGCTGG AGCTCAAACT CACACTGGAG AAACAAAACC AGGAGCTGTC CAAGGAACGA 
GAAGAATCTG AAAAACAGCT GAAGGAGATG AAGGACCAGC TGGAGGCTGA GCAGTACTTC 
ACGAAACTGT ACAAGACTCA GATTCGAGAG CTGAAAGAGG AAAGCGATGA AAAGGTTAAA 
CTCTACAAAG ATGCTCAGCA GAGAATAGAG GACCTGCAGG AAGAGAGGGA CTCGTTAGCG 
TCTCAGTTGG AGGTGAGCCT GACGAAGGCT GACTCTGAAC AGTTGGCTCG CATCACCGTT 
GAAGAGCAAT ACTCGGACCT GGAGAAAGAG AAGATCATGA AGGAACTGGA GATAAAGGAC 
ATGATCGCCA GACACCGGCA GGATCTTGCA GAAAAGGACG GTACCATCAA CTCGCTGGAG 
GAGTCAAACC GTACGCTGAC TGTGGATGTT GCCAACCTGG CCAGTGAGAA AGAGGAGCTC 
AACAATAAAC TGAAGCACAT ACAGCAAAAG CTGGAGAAGA TCAAAGAAGA AGAGAAACAG 
ATGAAATCTC TCACAGTGTC GTATGAGAAG CAGATACAGG TGGAGAAAAC ACTCAAGATT 
CAGGCTATAA ACAAGCTGGC GGAGGTCATG AAGAAGACGG ACGGCAGGCC ACACCAAATC 
AACAACACAG ACATCCGCAG GAAGGAGAAG GAGATCAGAC AACTCCAGCT GCAGCTCCGC 
ACGGAGAAAG AAAAGCTTAA CCACTCCATC ATCAAATACC AGAGAGAAAT CAACGACATG 
CAGGCTATGA TAGCAGAGGA GTCTCAGGTG CGTCTGGAGA TGCAGATGTC TCTGGACAGT 
AAAGACAGTG ATATTGAGCG TCTGCGCAGT CAACTCACGT CTCTGAGCAT TCACTCTCTG 
GACACCACCA GCATCAGCAG CATCGGCAAT GACCTGGATT CTGACGAGGC ATATCCAGTG 
CGAATCACAC ACTCACACAC CTCGGAGTCT ATGTCCTTCA CATACCAGCG AAGCTCCAAG 
TCGGTGTGTA TTGATACCCG GCCCTGTCGA ATCGCTCCTC GCTTCTCCTC TGACTCTGAT 
GAAGAGGAGG ATGAGGAAGA TGAGTGGAGA CAAACACCTG CCCTTACATA TGAGACGCCT 
CCTGACCCTG AGGCTGCAGC CGCAGAGTCC GGTCTTGAAG GATGGCTCTC AATGCCTGCC 
AAAAACACCA AACGGTTTGG ATGGGATAAG AAGTATGTGG TTGTGAGCAG CAAGAAAATC 
CTGTTTTATG ACTCTGAACA AGACCGAGAG CAGTCCAGTC CATTCATGAT CCTGGACATT 
GACAAACTCT TCCACGTGAG ACCCGTCACT CAGACAGATG TTTACCGCGC AGATCCCAGA 
GAAATCCCTC GCATCTTCCA GATACTATAT GCGAATGAAG GAGAGAGCAA GAAAGAGGAG 
CTGCCCGTGG AGTCGTTATC TGTGACTGAG AGGTCACTGT GTATCCCACA CAAGGGTCAC 
GAGTTTGTTC TGACGCTTTA TCACTTCCCC TCGAGCTGCG AGGTCTGTCC ACGACCCTTA 
TGGCACGTCT TCAGACCTCC GCCGGCTCTC GAGTGCAGAC GCTGTAGAGT CAAGTGCCAT 
AAAGACCACA TCGATCGCAA AGAAGAGGTC CTCGCTCCAT GTAGAGTGAA TTACGACATG 
TCCACAGCGA AAGAGATGCT GCTGCTGGCA AACTCCACAG AGGAGCAGAA GAAGTGGGTC 
AGTCGGCTGC TCAAGAGGGT TCCGCGTAAA CCTTCCATCG CCCACTCTTC CAGCATAGCC 
ATATCCGCTG CGCCCTCAGA GGCAACACCC ACTACCCTTT CCCCTCAGCC GTCGCCTCGC 
CTCGCTCAGT CTTCACCCAG ACTTTCACAC CGAGCAGCCG TCAAAGTGCA CTCTACACGA 
CAGCAGCCCT CCAGCAAGAA CAGATTGCTT GAAATGGGCC TGAAGGACTG GGGTTGGGCT 
CTTGAGGTTG AATTGGATGA TGACGATGTT TTAGACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rock2b ( XM_021468356.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324031.1 Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa39639
Clone ID Related Accession (Same CDS sequence)	XM_017352432.1 , XM_021468356.1
Accession Version	XM_021468356.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4482bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho-associated, coiled-coil containing protein kinase 2b isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGACCG GAGCGGGGAA GAGAATAGAG AGCCGGGTGA GAAAACTAGA GACGCTGATC 
AAGAACCAGA AATCACCGTT GAACTTGGAG AGTTTGCTGG ATTCAGTAAC TGCCCTTGCC 
CTTGATCTGA ACCATCCAGC ACTGAGAAAG AACAAAAACA TAGATGCCTT CTTAAGCAGA 
TATGAAAAAG CCGTGTGCAA CCTCAAGGAA CTACAGGTGA AGCTGGATGA TTTTGATCGA 
GTCAAGCTGA TCGGTCGTGG AGCGTTTGGC GCAGTGCAGC TGGTACGGCA CAAAGTATCT 
CAACAAGTTT ATGCCATGAA GCAGCTCAGC AAGTTTGAGA TGGTCAAGCG CTCTGACTCC 
GCCTTTTTCT GGGAGGAGCG TGACATCATG GCTTTCTCCC AGAGCCCTTG GATAGTTCAG 
CTGTGCTGTG CATTCCAAGA TGAAAAGTAC CTTTATTTGG TGATGGAGTT CATGCCAGGT 
GGAGATCTGG TGACTCTGAC CAGCAACTAT GACATCCCAG AGGAATGGGC TCAATTCTAT 
ACGGCAGAAG TGGTGCTGGC ACTCGACGCC ATCCATTCTC TGGGCTTCAT CCACAGAGAC 
ATCAAACCAG ACAACATGCT GCTGGACCGC AATGGACACC TCAAACTCGC AGACTTTGGC 
ACATGCATGA AGATGGACTC GACTGGTATG GTACGCTGTG ACACTGCTGT TGGGACACCT 
GATTACATTT CTCCAGAGGT TTTAATGTCT CAAGGAGGGA CTGGTTATTA CGGTCGCGAG 
TGTGACTGGT GGTCTGTTGG TGTTTTTATT TATGAGCTTC TAGTGGGCGA CACTCCATTT 
TATTCCGAGT CTCTGGTGGG CACATATGGA AAGATCATGG ATCACAAGAA CAGCTTAACT 
TTCCCAGATG ATATTGAAAT GTCAAAGAAT GCCAAAGACC TCATCTGTGC CTTTCTGAGT 
AGCAGGGAGG TGAGGCTTGG CCGAACTGGA GTGGATGAAA TAAAATGTCA CCCCTTCTTT 
AAGAATGATC AGTGGACCTT CGACACCATA CGAGACACCA TGGCTCCAGT GGTGCCAGAG 
CTGAGTAGCG ATATTGACAC CAGTAACTTT GATGATGACA TTAAGGATGA TCCGATAGGA 
ACAGAGACGT TCCCTCCGCC CCGTGCCTTC GCAGGGAACC AGCTACCATT TGTGGGTTTC 
ACCTATTTCA GGGAGGACCA ATTGTTAACA CAACAAAATA AGTGTTCAGA GGAAGATATC 
ATTGAAGATC ATGGCTTAAA CCACACAGAG TCTAACGACC TGGAGAAGAG GCTGAAAAAG 
TTGGAGGAAA AATTCAAACA TGAGATGCAA GCAAAGGAAG AACTGGAAAA TAAATGCCGA 
ATAGCAAATC AGCGGCTGGA GAAATTGTCC AAAGATCTTG AAGAAGAAGT GAATGCGAGA 
CAGGAGGCGG AAGACAACCT GAGATCCCTG GAGAAAGAGA AAGTTTTACT GAAACACCAG 
CGTACACAGA GCGTAAGAAA AGCTGGTCTA GAGACCGACA GAAAACGGCT GCTGGAGAAT 
GAAGTAAGCA GCCTGAAAGA GCAGCTTGCC GAACTGAAGA AAAAGAACCA GATCTCTCAT 
CTGTCTGCTG AGAAGAACAT TCACCTGGAA AGACAGCTGG AGGAGGTGAG TGCCAAGCTT 
CAGGCCGAGC TGGAGGAGTC GGAGAGGCTG AAGAAGGCCC AGATCGAGGC CTTTAGACAG 
TCCCAGCAGC TGGAGCTTTC CCTCAGGGAG CTGCAGGAGC GTCTGGCCCA GCTGGAGAAC 
AGCAGGCTGG TTCTGGAGCA GGACAAACTC AGCCTGCAGA CCTCGCTAGA GCTGGAGAAA 
CGAGAGAGAA ACGCCGGCTC AGAGACCATC ACTGACCTGC AGGGTCGTAT CTATGGACTG 
GAGGGTGAGT TGAAGCATAT AAAGAGTTCT CTATCAAAAG CTGAAGTTGA GAAAAGACAA 
TTACAAGAGG ATCTTACAAC CCTGGAAAAG GAGAAGAATA AGCAGGAGAT CGACCTCTCC 
TTCAAACTCA AAGCAGTTCA GCAGAGTCTG GAGCAGGAGG AGGCTGAACA TAAAACCACT 
AAAGCCAGAT TGGCCGACAA CAACAAAATC AACCAATCCA TAGAGGCAAA GTCTGAGACC 
CTGAAAGACA TGGAACATAA GCTGTTGGAA GAACGATCTG CGAAGCAACA ACTGGAAAAC 
AGACTGATGC AGCTGGAGAA GGAAAACTCT GTATTAGACT GTGATTACAA ACAAGCCAAA 
CATGAGCTTC AGGAGCTCCG CTCGCTCAAA GAAAATCTCA CAGAGCAGGT GGAGGTGCTG 
AATGTGCGGG TGCAGCAGGA GACACAGAGG AAAACTCTCT GTCAAGGTGA TCTCAAAGTT 
CAGAGGCAGG AGATCAACAG CCTGCGGTCA TCAGAACAAC AGCTCAAACA AGAACTCAAC 
CATCTGCTGG AGCTCAAACT CACACTGGAG AAACAAAACC AGGAGCTGTC CAAGGAACGA 
GAAGAATCTG AAAAACAGCT GAAGGAGATG AAGGACCAGC TGGAGGCTGA GCAGTACTTC 
ACGAAACTGT ACAAGACTCA GATTCGAGAG CTGAAAGAGG AAAGCGATGA AAAGGTTAAA 
CTCTACAAAG ATGCTCAGCA GAGAATAGAG GACCTGCAGG AAGAGAGGGA CTCGTTAGCG 
TCTCAGTTGG AGGTGAGCCT GACGAAGGCT GACTCTGAAC AGTTGGCTCG CATCACCGTT 
GAAGAGCAAT ACTCGGACCT GGAGAAAGAG AAGATCATGA AGGAACTGGA GATAAAGGAC 
ATGATCGCCA GACACCGGCA GGATCTTGCA GAAAAGGACG GTACCATCAA CTCGCTGGAG 
GAGTCAAACC GTACGCTGAC TGTGGATGTT GCCAACCTGG CCAGTGAGAA AGAGGAGCTC 
AACAATAAAC TGAAGCACAT ACAGCAAAAG CTGGAGAAGA TCAAAGAAGA AGAGAAACAG 
ATGAAATCTC TCACAGTGTC GTATGAGAAG CAGATACAGG TGGAGAAAAC ACTCAAGATT 
CAGGCTATAA ACAAGCTGGC GGAGGTCATG AAGAAGACGG ACGGCAGGCC ACACCAAATC 
AACAACACAG ACATCCGCAG GAAGGAGAAG GAGATCAGAC AACTCCAGCT GCAGCTCCGC 
ACGGAGAAAG AAAAGCTTAA CCACTCCATC ATCAAATACC AGAGAGAAAT CAACGACATG 
CAGGCTATGA TAGCAGAGGA GTCTCAGGTG CGTCTGGAGA TGCAGATGTC TCTGGACAGT 
AAAGACAGTG ATATTGAGCG TCTGCGCAGT CAACTCACGT CTCTGAGCAT TCACTCTCTG 
GACACCACCA GCATCAGCAG CATCGGCAAT GACCTGGATT CTGACGAGGC ATATCCAGTG 
CGAATCACAC ACTCACACAC CTCGGAGTCT ATGTCCTTCA CATACCAGCG AAGCTCCAAG 
TCGGTGTGTA TTGATACCCG GCCCTGTCGA ATCGCTCCTC GCTTCTCCTC TGACTCTGAT 
GAAGAGGAGG ATGAGGAAGA TGAGTGGAGA CAAACACCTG CCCTTACATA TGAGACGCCT 
CCTGACCCTG AGGCTGCAGC CGCAGAGTCC GGTCTTGAAG GATGGCTCTC AATGCCTGCC 
AAAAACACCA AACGGTTTGG ATGGGATAAG AAGTATGTGG TTGTGAGCAG CAAGAAAATC 
CTGTTTTATG ACTCTGAACA AGACCGAGAG CAGTCCAGTC CATTCATGAT CCTGGACATT 
GACAAACTCT TCCACGTGAG ACCCGTCACT CAGACAGATG TTTACCGCGC AGATCCCAGA 
GAAATCCCTC GCATCTTCCA GATACTATAT GCGAATGAAG GAGAGAGCAA GAAAGAGGAG 
CTGCCCGTGG AGTCGTTATC TGTGACTGAG AGGTCACTGT GTATCCCACA CAAGGGTCAC 
GAGTTTGTTC TGACGCTTTA TCACTTCCCC TCGAGCTGCG AGGTCTGTCC ACGACCCTTA 
TGGCACGTCT TCAGACCTCC GCCGGCTCTC GAGTGCAGAC GCTGTAGAGT CAAGTGCCAT 
AAAGACCACA TCGATCGCAA AGAAGAGGTC CTCGCTCCAT GTAGAGTGAA TTACGACATG 
TCCACAGCGA AAGAGATGCT GCTGCTGGCA AACTCCACAG AGGAGCAGAA GAAGTGGGTC 
AGTCGGCTGC TCAAGAGGGT TCCGCGTAAA CCTTCCATCG CCCACTCTTC CAGCATAGCC 
ATATCCGCTG CGCCCTCAGA GGCAACACCC ACTACCCTTT CCCCTCAGCC GTCGCCTCGC 
CTCGCTCAGT CTTCACCCAG ACTTTCACAC CGAGCAGCCG TCAAAGTGCA CTCTACACGA 
CAGCAGCCCT CCAGCAAGAA CAGATTGCTT GAAATGGGCC TGAAGGACTG GGGTTGGGCT 
CTTGAGGTTG AATTGGATGA TGACGATGTT TTAGACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324031.1
CDS	476..4957
Translation	MSTGAGKRIESRVRKLETLIKNQKSPLNLESLLDSVTALALDLNHPALRKNKNIDAFLSRYEKAVCNLKELQVKLDDFDRVKLIGRGAFGAVQLVRHKVSQQVYAMKQLSKFEMVKRSDSAFFWEERDIMAFSQSPWIVQLCCAFQDEKYLYLVMEFMPGGDLVTLTSNYDIPEEWAQFYTAEVVLALDAIHSLGFIHRDIKPDNMLLDRNGHLKLADFGTCMKMDSTGMVRCDTAVGTPDYISPEVLMSQGGTGYYGRECDWWSVGVFIYELLVGDTPFYSESLVGTYGKIMDHKNSLTFPDDIEMSKNAKDLICAFLSSREVRLGRTGVDEIKCHPFFKNDQWTFDTIRDTMAPVVPELSSDIDTSNFDDDIKDDPIGTETFPPPRAFAGNQLPFVGFTYFREDQLLTQQNKCSEEDIIEDHGLNHTESNDLEKRLKKLEEKFKHEMQAKEELENKCRIANQRLEKLSKDLEEEVNARQEAEDNLRSLEKEKVLLKHQRTQSVRKAGLETDRKRLLENEVSSLKEQLAELKKKNQISHLSAEKNIHLERQLEEVSAKLQAELEESERLKKAQIEAFRQSQQLELSLRELQERLAQLENSRLVLEQDKLSLQTSLELEKRERNAGSETITDLQGRIYGLEGELKHIKSSLSKAEVEKRQLQEDLTTLEKEKNKQEIDLSFKLKAVQQSLEQEEAEHKTTKARLADNNKINQSIEAKSETLKDMEHKLLEERSAKQQLENRLMQLEKENSVLDCDYKQAKHELQELRSLKENLTEQVEVLNVRVQQETQRKTLCQGDLKVQRQEINSLRSSEQQLKQELNHLLELKLTLEKQNQELSKEREESEKQLKEMKDQLEAEQYFTKLYKTQIRELKEESDEKVKLYKDAQQRIEDLQEERDSLASQLEVSLTKADSEQLARITVEEQYSDLEKEKIMKELEIKDMIARHRQDLAEKDGTINSLEESNRTLTVDVANLASEKEELNNKLKHIQQKLEKIKEEEKQMKSLTVSYEKQIQVEKTLKIQAINKLAEVMKKTDGRPHQINNTDIRRKEKEIRQLQLQLRTEKEKLNHSIIKYQREINDMQAMIAEESQVRLEMQMSLDSKDSDIERLRSQLTSLSIHSLDTTSISSIGNDLDSDEAYPVRITHSHTSESMSFTYQRSSKSVCIDTRPCRIAPRFSSDSDEEEDEEDEWRQTPALTYETPPDPEAAAAESGLEGWLSMPAKNTKRFGWDKKYVVVSSKKILFYDSEQDREQSSPFMILDIDKLFHVRPVTQTDVYRADPREIPRIFQILYANEGESKKEELPVESLSVTERSLCIPHKGHEFVLTLYHFPSSCEVCPRPLWHVFRPPPALECRRCRVKCHKDHIDRKEEVLAPCRVNYDMSTAKEMLLLANSTEEQKKWVSRLLKRVPRKPSIAHSSSIAISAAPSEATPTTLSPQPSPRLAQSSPRLSHRAAVKVHSTRQQPSSKNRLLEMGLKDWGWALEVELDDDDVLDF

Target ORF information:

RefSeq Version	XM_021468356.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468356.1

ORF Insert Sequence:

ATGTCGACCG GAGCGGGGAA GAGAATAGAG AGCCGGGTGA GAAAACTAGA GACGCTGATC 
AAGAACCAGA AATCACCGTT GAACTTGGAG AGTTTGCTGG ATTCAGTAAC TGCCCTTGCC 
CTTGATCTGA ACCATCCAGC ACTGAGAAAG AACAAAAACA TAGATGCCTT CTTAAGCAGA 
TATGAAAAAG CCGTGTGCAA CCTCAAGGAA CTACAGGTGA AGCTGGATGA TTTTGATCGA 
GTCAAGCTGA TCGGTCGTGG AGCGTTTGGC GCAGTGCAGC TGGTACGGCA CAAAGTATCT 
CAACAAGTTT ATGCCATGAA GCAGCTCAGC AAGTTTGAGA TGGTCAAGCG CTCTGACTCC 
GCCTTTTTCT GGGAGGAGCG TGACATCATG GCTTTCTCCC AGAGCCCTTG GATAGTTCAG 
CTGTGCTGTG CATTCCAAGA TGAAAAGTAC CTTTATTTGG TGATGGAGTT CATGCCAGGT 
GGAGATCTGG TGACTCTGAC CAGCAACTAT GACATCCCAG AGGAATGGGC TCAATTCTAT 
ACGGCAGAAG TGGTGCTGGC ACTCGACGCC ATCCATTCTC TGGGCTTCAT CCACAGAGAC 
ATCAAACCAG ACAACATGCT GCTGGACCGC AATGGACACC TCAAACTCGC AGACTTTGGC 
ACATGCATGA AGATGGACTC GACTGGTATG GTACGCTGTG ACACTGCTGT TGGGACACCT 
GATTACATTT CTCCAGAGGT TTTAATGTCT CAAGGAGGGA CTGGTTATTA CGGTCGCGAG 
TGTGACTGGT GGTCTGTTGG TGTTTTTATT TATGAGCTTC TAGTGGGCGA CACTCCATTT 
TATTCCGAGT CTCTGGTGGG CACATATGGA AAGATCATGG ATCACAAGAA CAGCTTAACT 
TTCCCAGATG ATATTGAAAT GTCAAAGAAT GCCAAAGACC TCATCTGTGC CTTTCTGAGT 
AGCAGGGAGG TGAGGCTTGG CCGAACTGGA GTGGATGAAA TAAAATGTCA CCCCTTCTTT 
AAGAATGATC AGTGGACCTT CGACACCATA CGAGACACCA TGGCTCCAGT GGTGCCAGAG 
CTGAGTAGCG ATATTGACAC CAGTAACTTT GATGATGACA TTAAGGATGA TCCGATAGGA 
ACAGAGACGT TCCCTCCGCC CCGTGCCTTC GCAGGGAACC AGCTACCATT TGTGGGTTTC 
ACCTATTTCA GGGAGGACCA ATTGTTAACA CAACAAAATA AGTGTTCAGA GGAAGATATC 
ATTGAAGATC ATGGCTTAAA CCACACAGAG TCTAACGACC TGGAGAAGAG GCTGAAAAAG 
TTGGAGGAAA AATTCAAACA TGAGATGCAA GCAAAGGAAG AACTGGAAAA TAAATGCCGA 
ATAGCAAATC AGCGGCTGGA GAAATTGTCC AAAGATCTTG AAGAAGAAGT GAATGCGAGA 
CAGGAGGCGG AAGACAACCT GAGATCCCTG GAGAAAGAGA AAGTTTTACT GAAACACCAG 
CGTACACAGA GCGTAAGAAA AGCTGGTCTA GAGACCGACA GAAAACGGCT GCTGGAGAAT 
GAAGTAAGCA GCCTGAAAGA GCAGCTTGCC GAACTGAAGA AAAAGAACCA GATCTCTCAT 
CTGTCTGCTG AGAAGAACAT TCACCTGGAA AGACAGCTGG AGGAGGTGAG TGCCAAGCTT 
CAGGCCGAGC TGGAGGAGTC GGAGAGGCTG AAGAAGGCCC AGATCGAGGC CTTTAGACAG 
TCCCAGCAGC TGGAGCTTTC CCTCAGGGAG CTGCAGGAGC GTCTGGCCCA GCTGGAGAAC 
AGCAGGCTGG TTCTGGAGCA GGACAAACTC AGCCTGCAGA CCTCGCTAGA GCTGGAGAAA 
CGAGAGAGAA ACGCCGGCTC AGAGACCATC ACTGACCTGC AGGGTCGTAT CTATGGACTG 
GAGGGTGAGT TGAAGCATAT AAAGAGTTCT CTATCAAAAG CTGAAGTTGA GAAAAGACAA 
TTACAAGAGG ATCTTACAAC CCTGGAAAAG GAGAAGAATA AGCAGGAGAT CGACCTCTCC 
TTCAAACTCA AAGCAGTTCA GCAGAGTCTG GAGCAGGAGG AGGCTGAACA TAAAACCACT 
AAAGCCAGAT TGGCCGACAA CAACAAAATC AACCAATCCA TAGAGGCAAA GTCTGAGACC 
CTGAAAGACA TGGAACATAA GCTGTTGGAA GAACGATCTG CGAAGCAACA ACTGGAAAAC 
AGACTGATGC AGCTGGAGAA GGAAAACTCT GTATTAGACT GTGATTACAA ACAAGCCAAA 
CATGAGCTTC AGGAGCTCCG CTCGCTCAAA GAAAATCTCA CAGAGCAGGT GGAGGTGCTG 
AATGTGCGGG TGCAGCAGGA GACACAGAGG AAAACTCTCT GTCAAGGTGA TCTCAAAGTT 
CAGAGGCAGG AGATCAACAG CCTGCGGTCA TCAGAACAAC AGCTCAAACA AGAACTCAAC 
CATCTGCTGG AGCTCAAACT CACACTGGAG AAACAAAACC AGGAGCTGTC CAAGGAACGA 
GAAGAATCTG AAAAACAGCT GAAGGAGATG AAGGACCAGC TGGAGGCTGA GCAGTACTTC 
ACGAAACTGT ACAAGACTCA GATTCGAGAG CTGAAAGAGG AAAGCGATGA AAAGGTTAAA 
CTCTACAAAG ATGCTCAGCA GAGAATAGAG GACCTGCAGG AAGAGAGGGA CTCGTTAGCG 
TCTCAGTTGG AGGTGAGCCT GACGAAGGCT GACTCTGAAC AGTTGGCTCG CATCACCGTT 
GAAGAGCAAT ACTCGGACCT GGAGAAAGAG AAGATCATGA AGGAACTGGA GATAAAGGAC 
ATGATCGCCA GACACCGGCA GGATCTTGCA GAAAAGGACG GTACCATCAA CTCGCTGGAG 
GAGTCAAACC GTACGCTGAC TGTGGATGTT GCCAACCTGG CCAGTGAGAA AGAGGAGCTC 
AACAATAAAC TGAAGCACAT ACAGCAAAAG CTGGAGAAGA TCAAAGAAGA AGAGAAACAG 
ATGAAATCTC TCACAGTGTC GTATGAGAAG CAGATACAGG TGGAGAAAAC ACTCAAGATT 
CAGGCTATAA ACAAGCTGGC GGAGGTCATG AAGAAGACGG ACGGCAGGCC ACACCAAATC 
AACAACACAG ACATCCGCAG GAAGGAGAAG GAGATCAGAC AACTCCAGCT GCAGCTCCGC 
ACGGAGAAAG AAAAGCTTAA CCACTCCATC ATCAAATACC AGAGAGAAAT CAACGACATG 
CAGGCTATGA TAGCAGAGGA GTCTCAGGTG CGTCTGGAGA TGCAGATGTC TCTGGACAGT 
AAAGACAGTG ATATTGAGCG TCTGCGCAGT CAACTCACGT CTCTGAGCAT TCACTCTCTG 
GACACCACCA GCATCAGCAG CATCGGCAAT GACCTGGATT CTGACGAGGC ATATCCAGTG 
CGAATCACAC ACTCACACAC CTCGGAGTCT ATGTCCTTCA CATACCAGCG AAGCTCCAAG 
TCGGTGTGTA TTGATACCCG GCCCTGTCGA ATCGCTCCTC GCTTCTCCTC TGACTCTGAT 
GAAGAGGAGG ATGAGGAAGA TGAGTGGAGA CAAACACCTG CCCTTACATA TGAGACGCCT 
CCTGACCCTG AGGCTGCAGC CGCAGAGTCC GGTCTTGAAG GATGGCTCTC AATGCCTGCC 
AAAAACACCA AACGGTTTGG ATGGGATAAG AAGTATGTGG TTGTGAGCAG CAAGAAAATC 
CTGTTTTATG ACTCTGAACA AGACCGAGAG CAGTCCAGTC CATTCATGAT CCTGGACATT 
GACAAACTCT TCCACGTGAG ACCCGTCACT CAGACAGATG TTTACCGCGC AGATCCCAGA 
GAAATCCCTC GCATCTTCCA GATACTATAT GCGAATGAAG GAGAGAGCAA GAAAGAGGAG 
CTGCCCGTGG AGTCGTTATC TGTGACTGAG AGGTCACTGT GTATCCCACA CAAGGGTCAC 
GAGTTTGTTC TGACGCTTTA TCACTTCCCC TCGAGCTGCG AGGTCTGTCC ACGACCCTTA 
TGGCACGTCT TCAGACCTCC GCCGGCTCTC GAGTGCAGAC GCTGTAGAGT CAAGTGCCAT 
AAAGACCACA TCGATCGCAA AGAAGAGGTC CTCGCTCCAT GTAGAGTGAA TTACGACATG 
TCCACAGCGA AAGAGATGCT GCTGCTGGCA AACTCCACAG AGGAGCAGAA GAAGTGGGTC 
AGTCGGCTGC TCAAGAGGGT TCCGCGTAAA CCTTCCATCG CCCACTCTTC CAGCATAGCC 
ATATCCGCTG CGCCCTCAGA GGCAACACCC ACTACCCTTT CCCCTCAGCC GTCGCCTCGC 
CTCGCTCAGT CTTCACCCAG ACTTTCACAC CGAGCAGCCG TCAAAGTGCA CTCTACACGA 
CAGCAGCCCT CCAGCAAGAA CAGATTGCTT GAAATGGGCC TGAAGGACTG GGGTTGGGCT 
CTTGAGGTTG AATTGGATGA TGACGATGTT TTAGACTTCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rock2b ( XM_017352433.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207922.1 Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X2, mRNA.

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CloneID	ODa39640
Clone ID Related Accession (Same CDS sequence)	XM_017352433.1 , XM_017352433.2
Accession Version	XM_017352433.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	rho-associated, coiled-coil containing protein kinase 2b isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGACCG GAGCGGGGAA GAGAATAGAG AGCCGGGTGA GAAAACTAGA GACGCTGATC 
AAGAACCAGA AATCACCGTT GAACTTGGAG AGTTTGCTGG ATTCAGTAAC TGCCCTTGCC 
CTTGATCTGA ACCATCCAGC ACTGAGAAAG AACAAAAACA TAGATGCCTT CTTAAGCAGA 
TATGAAAAAG CCGTGTGCAA CCTCAAGGAA CTACAGGTGA AGCTGGATGA TTTTGATCGA 
GTCAAGCTGA TCGGTCGTGG AGCGTTTGGC GCAGTGCAGC TGGTACGGCA CAAAGTATCT 
CAACAAGTTT ATGCCATGAA GCAGCTCAGC AAGTTTGAGA TGGTCAAGCG CTCTGACTCC 
GCCTTTTTCT GGGAGGAGCG TGACATCATG GCTTTCTCCC AGAGCCCTTG GATAGTTCAG 
CTGTGCTGTG CATTCCAAGA TGAAAAGTAC CTTTATTTGG TGATGGAGTT CATGCCAGGT 
GGAGATCTGG TGACTCTGAC CAGCAACTAT GACATCCCAG AGGAATGGGC TCAATTCTAT 
ACGGCAGAAG TGGTGCTGGC ACTCGACGCC ATCCATTCTC TGGGCTTCAT CCACAGAGAC 
ATCAAACCAG ACAACATGCT GCTGGACCGC AATGGACACC TCAAACTCGC AGACTTTGGC 
ACATGCATGA AGATGGACTC GACTGGTATG GTACGCTGTG ACACTGCTGT TGGGACACCT 
GATTACATTT CTCCAGAGGT TTTAATGTCT CAAGGAGGGA CTGGTTATTA CGGTCGCGAG 
TGTGACTGGT GGTCTGTTGG TGTTTTTATT TATGAGCTTC TAGTGGGCGA CACTCCATTT 
TATTCCGAGT CTCTGGTGGG CACATATGGA AAGATCATGG ATCACAAGAA CAGCTTAACT 
TTCCCAGATG ATATTGAAAT GTCAAAGAAT GCCAAAGACC TCATCTGTGC CTTTCTGAGT 
AGCAGGGAGG TGAGGCTTGG CCGAACTGGA GTGGATGAAA TAAAATGTCA CCCCTTCTTT 
AAGAATGATC AGTGGACCTT CGACACCATA CGAGACACCA TGGCTCCAGT GGTGCCAGAG 
CTGAGTAGCG ATATTGACAC CAGTAACTTT GATGATGACA TTAAGGATGA TCCGATAGGA 
ACAGAGACGT TCCCTCCGCC CCGTGCCTTC GCAGGGAACC AGCTACCATT TGTGGGTTTC 
ACCTATTTCA GGGAGGACCA ATTGTTAACA CAACAAAATA AGTGTTCAGA GGAAGATATC 
ATTGAAGATC ATGGCTTAAA CCACACAGAG TCTAACGACC TGGAGAAGAG GCTGAAAAAG 
TTGGAGGAAA AATTCAAACA TGAGATGCAA GCAAAGGAAG AACTGGAAAA TAAATGCCGA 
ATAGCAAATC AGCGGCTGGA GAAATTGTCC AAAGATCTTG AAGAAGAAGT GAATGCGAGA 
CAGGAGGCGG AAGACAACCT GAGATCCCTG GAGAAAGAGA AAGTTTTACT GAAACACCAG 
CGTACACAGA GCGTAAGAAA AGCTGGTCTA GAGACCGACA GAAAACGGCT GCTGGAGAAT 
GAAGTAAGCA GCCTGAAAGA GCAGCTTGCC GAACTGAAGA AAAAGAACCA GATCTCTCAT 
CTGTCTGCTG AGAAGAACAT TCACCTGGAA AGACAGCTGG AGGAGGTGAG TGCCAAGCTT 
CAGGCCGAGC TGGAGGAGTC GGAGAGGCTG AAGAAGGCCC AGATCGAGGC CTTTAGACAG 
TCCCAGCAGC TGGAGCTTTC CCTCAGGGAG CTGCAGGAGC GTCTGGCCCA GCTGGAGAAC 
AGCAGGCTGG TTCTGGAGCA GGACAAACTC AGCCTGCAGA CCTCGCTAGA GCTGGAGAAA 
CGAGAGAGAA ACGCCGGCTC AGAGACCATC ACTGACCTGC AGGGTCGTAT CTATGGACTG 
GAGGGTGAGT TGAAGCATAT AAAGAGTTCT CTATCAAAAG CTGAAGTTGA GAAAAGACAA 
TTACAAGAGG ATCTTACAAC CCTGGAAAAG GAGAAGAATA AGCAGGAGAT CGACCTCTCC 
TTCAAACTCA AAGCAGTTCA GCAGAGTCTG GAGCAGGAGG AGGCTGAACA TAAAACCACT 
AAAGCCAGAT TGGCCGACAA CAACAAAATC AACCAATCCA TAGAGGCAAA GTCTGAGACC 
CTGAAAGACA TGGAACATAA GCTGTTGGAA GAACGATCTG CGAAGCAACA ACTGGAAAAC 
AGACTGATGC AGCTGGAGAA GGAAAACTCT GTATTAGACT GTGATTACAA ACAAGCCAAA 
CATGAGCTTC AGGAGCTCCG CTCGCTCAAA GAAAATCTCA CAGAGCAGGT GGAGGTGCTG 
AATGTGCGGG TGCAGCAGGA GACACAGAGG AAAACTCTCT GTCAAGGTGA TCTCAAAGTT 
CAGAGGCAGG AGATCAACAG CCTGCGGTCA TCAGAACAAC AGCTCAAACA AGAACTCAAC 
CATCTGCTGG AGCTCAAACT CACACTGGAG AAACAAAACC AGGAGCTGTC CAAGGAACGA 
GAAGAATCTG AAAAACAGCT GAAGGAGATG AAGGACCAGC TGGAGGCTGA GCAGTACTTC 
ACGAAACTGT ACAAGACTCA GATTCGAGAG CTGAAAGAGG AAAGCGATGA AAAGGTTAAA 
CTCTACAAAG ATGCTCAGCA GAGAATAGAG GACCTGCAGG AAGAGAGGGA CTCGTTAGCG 
TCTCAGTTGG AGGTGAGCCT GACGAAGGCT GACTCTGAAC AGTTGGCTCG CATCACCGTT 
GAAGAGCAAT ACTCGGACCT GGAGAAAGAG AAGATCATGA AGGAACTGGA GATAAAGGAC 
ATGATCGCCA GACACCGGCA GGATCTTGCA GAAAAGGACG GTACCATCAA CTCGCTGGAG 
GAGTCAAACC GTACGCTGAC TGTGGATGTT GCCAACCTGG CCAGTGAGAA AGAGGAGCTC 
AACAATAAAC TGAAGCACAT ACAGCAAAAG CTGGAGAAGA TCAAAGAAGA AGAGAAACAG 
ATGAAATCTC TCACAGTGTC GTATGAGAAG CAGATACAGG TGGAGAAAAC ACTCAAGATT 
CAGGCTATAA ACAAGCTGGC GGAGGTCATG AAGAAGACGG ACGGCAGGCC ACACCAAATC 
AACAACACAG ACATCCGCAG GAAGGAGAAG GAGATCAGAC AACTCCAGCT GCAGCTCCGC 
ACGGAGAAAG AAAAGCTTAA CCACTCCATC ATCAAATACC AGAGAGAAAT CAACGACATG 
CAGGCTATGA TAGCAGAGGA GTCTCAGGTG CGTCTGGAGA TGCAGATGTC TCTGGACAGT 
AAAGACAGTG ATATTGAGCG TCTGCGCAGT CAACTCACGT CTCTGAGCAT TCACTCTCTG 
GACACCACCA GCATCAGCAG CATCGGCAAT GACCTGGATT CTGACGAGGC ATATCCAGTG 
CGAATCACAC ACTCACACAC CTCGGAGTCT ATGTCCTTCA CATACCAGCG AAGCTCCAAG 
TCGGTGTGTA TTGATACCCG GCCCTGTCGA ATCGCTCCTC GCTTCTCCTC TGACTCTGAT 
GAAGAGGAGG ATGAGGAAGA TGAGTGGAGA CAAACACCTG CCCTTACATA TGAGACGCCT 
CCTGACCCTG AGGCTGCAGC CGCAGAGTCC GGTCTTGAAG GATGGCTCTC AATGCCTGCC 
AAAAACACCA AACGGTTTGG ATGGGATAAG AAGTATGTGG TTGTGAGCAG CAAGAAAATC 
CTGTTTTATG ACTCTGAACA AGACCGAGAG CAGTCCAGTC CATTCATGAT CCTGGACATT 
GACAAACTCT TCCACGTGAG ACCCGTCACT CAGACAGATG TTTACCGCGC AGATCCCAGA 
GAAATCCCTC GCATCTTCCA GATACTATAT GCGAATGAAG GAGAGAGCAA GAAAGAGGAG 
CTGCCCGTGG AGTCGTTATC TGTGACTGAG AGGTCACTGT GTATCCCACA CAAGGGTCAC 
GAGTTTGTTC TGACGCTTTA TCACTTCCCC TCGAGCTGCG AGGTCTGTCC ACGACCCTTA 
TGGCACGTCT TCAGACCTCC GCCGGCTCTC GAGTGCAGAC GCTGTAGAGT CAAGTGCCAT 
AAAGACCACA TCGATCGCAA AGAAGAGGTC CTCGCTCCAT GTAGAGTGAA TTACGACATG 
TCCACAGCGA AAGAGATGCT GCTGCTGGCA AACTCCACAG AGGAGCAGAA GAAGTGGGTC 
AGTCGGCTGC TCAAGAGGGT TCCGCGTAAA CCTTCCATCG CCCACTCTTC CAGCATAGCC 
ATATCCGCTG CGCCCTCAGA GGCAACACCC ACTACCCTTT CCCCTCAGCC GTCGCCTCGC 
CTCGCTCAGT CTTCACCCAG ACTTTCACAC CGAGCAGCCG TCAAAGTGCA CTCTACACGA 
CAGCAGCCCT CCAGCAAGAA CAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207922.1
CDS	585..4991
Translation	MSTGAGKRIESRVRKLETLIKNQKSPLNLESLLDSVTALALDLNHPALRKNKNIDAFLSRYEKAVCNLKELQVKLDDFDRVKLIGRGAFGAVQLVRHKVSQQVYAMKQLSKFEMVKRSDSAFFWEERDIMAFSQSPWIVQLCCAFQDEKYLYLVMEFMPGGDLVTLTSNYDIPEEWAQFYTAEVVLALDAIHSLGFIHRDIKPDNMLLDRNGHLKLADFGTCMKMDSTGMVRCDTAVGTPDYISPEVLMSQGGTGYYGRECDWWSVGVFIYELLVGDTPFYSESLVGTYGKIMDHKNSLTFPDDIEMSKNAKDLICAFLSSREVRLGRTGVDEIKCHPFFKNDQWTFDTIRDTMAPVVPELSSDIDTSNFDDDIKDDPIGTETFPPPRAFAGNQLPFVGFTYFREDQLLTQQNKCSEEDIIEDHGLNHTESNDLEKRLKKLEEKFKHEMQAKEELENKCRIANQRLEKLSKDLEEEVNARQEAEDNLRSLEKEKVLLKHQRTQSVRKAGLETDRKRLLENEVSSLKEQLAELKKKNQISHLSAEKNIHLERQLEEVSAKLQAELEESERLKKAQIEAFRQSQQLELSLRELQERLAQLENSRLVLEQDKLSLQTSLELEKRERNAGSETITDLQGRIYGLEGELKHIKSSLSKAEVEKRQLQEDLTTLEKEKNKQEIDLSFKLKAVQQSLEQEEAEHKTTKARLADNNKINQSIEAKSETLKDMEHKLLEERSAKQQLENRLMQLEKENSVLDCDYKQAKHELQELRSLKENLTEQVEVLNVRVQQETQRKTLCQGDLKVQRQEINSLRSSEQQLKQELNHLLELKLTLEKQNQELSKEREESEKQLKEMKDQLEAEQYFTKLYKTQIRELKEESDEKVKLYKDAQQRIEDLQEERDSLASQLEVSLTKADSEQLARITVEEQYSDLEKEKIMKELEIKDMIARHRQDLAEKDGTINSLEESNRTLTVDVANLASEKEELNNKLKHIQQKLEKIKEEEKQMKSLTVSYEKQIQVEKTLKIQAINKLAEVMKKTDGRPHQINNTDIRRKEKEIRQLQLQLRTEKEKLNHSIIKYQREINDMQAMIAEESQVRLEMQMSLDSKDSDIERLRSQLTSLSIHSLDTTSISSIGNDLDSDEAYPVRITHSHTSESMSFTYQRSSKSVCIDTRPCRIAPRFSSDSDEEEDEEDEWRQTPALTYETPPDPEAAAAESGLEGWLSMPAKNTKRFGWDKKYVVVSSKKILFYDSEQDREQSSPFMILDIDKLFHVRPVTQTDVYRADPREIPRIFQILYANEGESKKEELPVESLSVTERSLCIPHKGHEFVLTLYHFPSSCEVCPRPLWHVFRPPPALECRRCRVKCHKDHIDRKEEVLAPCRVNYDMSTAKEMLLLANSTEEQKKWVSRLLKRVPRKPSIAHSSSIAISAAPSEATPTTLSPQPSPRLAQSSPRLSHRAAVKVHSTRQQPSSKNS

Target ORF information:

RefSeq Version	XM_017352433.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352433.1

ORF Insert Sequence:

ATGTCGACCG GAGCGGGGAA GAGAATAGAG AGCCGGGTGA GAAAACTAGA GACGCTGATC 
AAGAACCAGA AATCACCGTT GAACTTGGAG AGTTTGCTGG ATTCAGTAAC TGCCCTTGCC 
CTTGATCTGA ACCATCCAGC ACTGAGAAAG AACAAAAACA TAGATGCCTT CTTAAGCAGA 
TATGAAAAAG CCGTGTGCAA CCTCAAGGAA CTACAGGTGA AGCTGGATGA TTTTGATCGA 
GTCAAGCTGA TCGGTCGTGG AGCGTTTGGC GCAGTGCAGC TGGTACGGCA CAAAGTATCT 
CAACAAGTTT ATGCCATGAA GCAGCTCAGC AAGTTTGAGA TGGTCAAGCG CTCTGACTCC 
GCCTTTTTCT GGGAGGAGCG TGACATCATG GCTTTCTCCC AGAGCCCTTG GATAGTTCAG 
CTGTGCTGTG CATTCCAAGA TGAAAAGTAC CTTTATTTGG TGATGGAGTT CATGCCAGGT 
GGAGATCTGG TGACTCTGAC CAGCAACTAT GACATCCCAG AGGAATGGGC TCAATTCTAT 
ACGGCAGAAG TGGTGCTGGC ACTCGACGCC ATCCATTCTC TGGGCTTCAT CCACAGAGAC 
ATCAAACCAG ACAACATGCT GCTGGACCGC AATGGACACC TCAAACTCGC AGACTTTGGC 
ACATGCATGA AGATGGACTC GACTGGTATG GTACGCTGTG ACACTGCTGT TGGGACACCT 
GATTACATTT CTCCAGAGGT TTTAATGTCT CAAGGAGGGA CTGGTTATTA CGGTCGCGAG 
TGTGACTGGT GGTCTGTTGG TGTTTTTATT TATGAGCTTC TAGTGGGCGA CACTCCATTT 
TATTCCGAGT CTCTGGTGGG CACATATGGA AAGATCATGG ATCACAAGAA CAGCTTAACT 
TTCCCAGATG ATATTGAAAT GTCAAAGAAT GCCAAAGACC TCATCTGTGC CTTTCTGAGT 
AGCAGGGAGG TGAGGCTTGG CCGAACTGGA GTGGATGAAA TAAAATGTCA CCCCTTCTTT 
AAGAATGATC AGTGGACCTT CGACACCATA CGAGACACCA TGGCTCCAGT GGTGCCAGAG 
CTGAGTAGCG ATATTGACAC CAGTAACTTT GATGATGACA TTAAGGATGA TCCGATAGGA 
ACAGAGACGT TCCCTCCGCC CCGTGCCTTC GCAGGGAACC AGCTACCATT TGTGGGTTTC 
ACCTATTTCA GGGAGGACCA ATTGTTAACA CAACAAAATA AGTGTTCAGA GGAAGATATC 
ATTGAAGATC ATGGCTTAAA CCACACAGAG TCTAACGACC TGGAGAAGAG GCTGAAAAAG 
TTGGAGGAAA AATTCAAACA TGAGATGCAA GCAAAGGAAG AACTGGAAAA TAAATGCCGA 
ATAGCAAATC AGCGGCTGGA GAAATTGTCC AAAGATCTTG AAGAAGAAGT GAATGCGAGA 
CAGGAGGCGG AAGACAACCT GAGATCCCTG GAGAAAGAGA AAGTTTTACT GAAACACCAG 
CGTACACAGA GCGTAAGAAA AGCTGGTCTA GAGACCGACA GAAAACGGCT GCTGGAGAAT 
GAAGTAAGCA GCCTGAAAGA GCAGCTTGCC GAACTGAAGA AAAAGAACCA GATCTCTCAT 
CTGTCTGCTG AGAAGAACAT TCACCTGGAA AGACAGCTGG AGGAGGTGAG TGCCAAGCTT 
CAGGCCGAGC TGGAGGAGTC GGAGAGGCTG AAGAAGGCCC AGATCGAGGC CTTTAGACAG 
TCCCAGCAGC TGGAGCTTTC CCTCAGGGAG CTGCAGGAGC GTCTGGCCCA GCTGGAGAAC 
AGCAGGCTGG TTCTGGAGCA GGACAAACTC AGCCTGCAGA CCTCGCTAGA GCTGGAGAAA 
CGAGAGAGAA ACGCCGGCTC AGAGACCATC ACTGACCTGC AGGGTCGTAT CTATGGACTG 
GAGGGTGAGT TGAAGCATAT AAAGAGTTCT CTATCAAAAG CTGAAGTTGA GAAAAGACAA 
TTACAAGAGG ATCTTACAAC CCTGGAAAAG GAGAAGAATA AGCAGGAGAT CGACCTCTCC 
TTCAAACTCA AAGCAGTTCA GCAGAGTCTG GAGCAGGAGG AGGCTGAACA TAAAACCACT 
AAAGCCAGAT TGGCCGACAA CAACAAAATC AACCAATCCA TAGAGGCAAA GTCTGAGACC 
CTGAAAGACA TGGAACATAA GCTGTTGGAA GAACGATCTG CGAAGCAACA ACTGGAAAAC 
AGACTGATGC AGCTGGAGAA GGAAAACTCT GTATTAGACT GTGATTACAA ACAAGCCAAA 
CATGAGCTTC AGGAGCTCCG CTCGCTCAAA GAAAATCTCA CAGAGCAGGT GGAGGTGCTG 
AATGTGCGGG TGCAGCAGGA GACACAGAGG AAAACTCTCT GTCAAGGTGA TCTCAAAGTT 
CAGAGGCAGG AGATCAACAG CCTGCGGTCA TCAGAACAAC AGCTCAAACA AGAACTCAAC 
CATCTGCTGG AGCTCAAACT CACACTGGAG AAACAAAACC AGGAGCTGTC CAAGGAACGA 
GAAGAATCTG AAAAACAGCT GAAGGAGATG AAGGACCAGC TGGAGGCTGA GCAGTACTTC 
ACGAAACTGT ACAAGACTCA GATTCGAGAG CTGAAAGAGG AAAGCGATGA AAAGGTTAAA 
CTCTACAAAG ATGCTCAGCA GAGAATAGAG GACCTGCAGG AAGAGAGGGA CTCGTTAGCG 
TCTCAGTTGG AGGTGAGCCT GACGAAGGCT GACTCTGAAC AGTTGGCTCG CATCACCGTT 
GAAGAGCAAT ACTCGGACCT GGAGAAAGAG AAGATCATGA AGGAACTGGA GATAAAGGAC 
ATGATCGCCA GACACCGGCA GGATCTTGCA GAAAAGGACG GTACCATCAA CTCGCTGGAG 
GAGTCAAACC GTACGCTGAC TGTGGATGTT GCCAACCTGG CCAGTGAGAA AGAGGAGCTC 
AACAATAAAC TGAAGCACAT ACAGCAAAAG CTGGAGAAGA TCAAAGAAGA AGAGAAACAG 
ATGAAATCTC TCACAGTGTC GTATGAGAAG CAGATACAGG TGGAGAAAAC ACTCAAGATT 
CAGGCTATAA ACAAGCTGGC GGAGGTCATG AAGAAGACGG ACGGCAGGCC ACACCAAATC 
AACAACACAG ACATCCGCAG GAAGGAGAAG GAGATCAGAC AACTCCAGCT GCAGCTCCGC 
ACGGAGAAAG AAAAGCTTAA CCACTCCATC ATCAAATACC AGAGAGAAAT CAACGACATG 
CAGGCTATGA TAGCAGAGGA GTCTCAGGTG CGTCTGGAGA TGCAGATGTC TCTGGACAGT 
AAAGACAGTG ATATTGAGCG TCTGCGCAGT CAACTCACGT CTCTGAGCAT TCACTCTCTG 
GACACCACCA GCATCAGCAG CATCGGCAAT GACCTGGATT CTGACGAGGC ATATCCAGTG 
CGAATCACAC ACTCACACAC CTCGGAGTCT ATGTCCTTCA CATACCAGCG AAGCTCCAAG 
TCGGTGTGTA TTGATACCCG GCCCTGTCGA ATCGCTCCTC GCTTCTCCTC TGACTCTGAT 
GAAGAGGAGG ATGAGGAAGA TGAGTGGAGA CAAACACCTG CCCTTACATA TGAGACGCCT 
CCTGACCCTG AGGCTGCAGC CGCAGAGTCC GGTCTTGAAG GATGGCTCTC AATGCCTGCC 
AAAAACACCA AACGGTTTGG ATGGGATAAG AAGTATGTGG TTGTGAGCAG CAAGAAAATC 
CTGTTTTATG ACTCTGAACA AGACCGAGAG CAGTCCAGTC CATTCATGAT CCTGGACATT 
GACAAACTCT TCCACGTGAG ACCCGTCACT CAGACAGATG TTTACCGCGC AGATCCCAGA 
GAAATCCCTC GCATCTTCCA GATACTATAT GCGAATGAAG GAGAGAGCAA GAAAGAGGAG 
CTGCCCGTGG AGTCGTTATC TGTGACTGAG AGGTCACTGT GTATCCCACA CAAGGGTCAC 
GAGTTTGTTC TGACGCTTTA TCACTTCCCC TCGAGCTGCG AGGTCTGTCC ACGACCCTTA 
TGGCACGTCT TCAGACCTCC GCCGGCTCTC GAGTGCAGAC GCTGTAGAGT CAAGTGCCAT 
AAAGACCACA TCGATCGCAA AGAAGAGGTC CTCGCTCCAT GTAGAGTGAA TTACGACATG 
TCCACAGCGA AAGAGATGCT GCTGCTGGCA AACTCCACAG AGGAGCAGAA GAAGTGGGTC 
AGTCGGCTGC TCAAGAGGGT TCCGCGTAAA CCTTCCATCG CCCACTCTTC CAGCATAGCC 
ATATCCGCTG CGCCCTCAGA GGCAACACCC ACTACCCTTT CCCCTCAGCC GTCGCCTCGC 
CTCGCTCAGT CTTCACCCAG ACTTTCACAC CGAGCAGCCG TCAAAGTGCA CTCTACACGA 
CAGCAGCCCT CCAGCAAGAA CAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rock2b ( XM_017352433.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207922.1 Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X5, mRNA.

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CloneID	ODa39640
Clone ID Related Accession (Same CDS sequence)	XM_017352433.1 , XM_017352433.2
Accession Version	XM_017352433.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4407bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho-associated, coiled-coil containing protein kinase 2b isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352433.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGACCG GAGCGGGGAA GAGAATAGAG AGCCGGGTGA GAAAACTAGA GACGCTGATC 
AAGAACCAGA AATCACCGTT GAACTTGGAG AGTTTGCTGG ATTCAGTAAC TGCCCTTGCC 
CTTGATCTGA ACCATCCAGC ACTGAGAAAG AACAAAAACA TAGATGCCTT CTTAAGCAGA 
TATGAAAAAG CCGTGTGCAA CCTCAAGGAA CTACAGGTGA AGCTGGATGA TTTTGATCGA 
GTCAAGCTGA TCGGTCGTGG AGCGTTTGGC GCAGTGCAGC TGGTACGGCA CAAAGTATCT 
CAACAAGTTT ATGCCATGAA GCAGCTCAGC AAGTTTGAGA TGGTCAAGCG CTCTGACTCC 
GCCTTTTTCT GGGAGGAGCG TGACATCATG GCTTTCTCCC AGAGCCCTTG GATAGTTCAG 
CTGTGCTGTG CATTCCAAGA TGAAAAGTAC CTTTATTTGG TGATGGAGTT CATGCCAGGT 
GGAGATCTGG TGACTCTGAC CAGCAACTAT GACATCCCAG AGGAATGGGC TCAATTCTAT 
ACGGCAGAAG TGGTGCTGGC ACTCGACGCC ATCCATTCTC TGGGCTTCAT CCACAGAGAC 
ATCAAACCAG ACAACATGCT GCTGGACCGC AATGGACACC TCAAACTCGC AGACTTTGGC 
ACATGCATGA AGATGGACTC GACTGGTATG GTACGCTGTG ACACTGCTGT TGGGACACCT 
GATTACATTT CTCCAGAGGT TTTAATGTCT CAAGGAGGGA CTGGTTATTA CGGTCGCGAG 
TGTGACTGGT GGTCTGTTGG TGTTTTTATT TATGAGCTTC TAGTGGGCGA CACTCCATTT 
TATTCCGAGT CTCTGGTGGG CACATATGGA AAGATCATGG ATCACAAGAA CAGCTTAACT 
TTCCCAGATG ATATTGAAAT GTCAAAGAAT GCCAAAGACC TCATCTGTGC CTTTCTGAGT 
AGCAGGGAGG TGAGGCTTGG CCGAACTGGA GTGGATGAAA TAAAATGTCA CCCCTTCTTT 
AAGAATGATC AGTGGACCTT CGACACCATA CGAGACACCA TGGCTCCAGT GGTGCCAGAG 
CTGAGTAGCG ATATTGACAC CAGTAACTTT GATGATGACA TTAAGGATGA TCCGATAGGA 
ACAGAGACGT TCCCTCCGCC CCGTGCCTTC GCAGGGAACC AGCTACCATT TGTGGGTTTC 
ACCTATTTCA GGGAGGACCA ATTGTTAACA CAACAAAATA AGTGTTCAGA GGAAGATATC 
ATTGAAGATC ATGGCTTAAA CCACACAGAG TCTAACGACC TGGAGAAGAG GCTGAAAAAG 
TTGGAGGAAA AATTCAAACA TGAGATGCAA GCAAAGGAAG AACTGGAAAA TAAATGCCGA 
ATAGCAAATC AGCGGCTGGA GAAATTGTCC AAAGATCTTG AAGAAGAAGT GAATGCGAGA 
CAGGAGGCGG AAGACAACCT GAGATCCCTG GAGAAAGAGA AAGTTTTACT GAAACACCAG 
CGTACACAGA GCGTAAGAAA AGCTGGTCTA GAGACCGACA GAAAACGGCT GCTGGAGAAT 
GAAGTAAGCA GCCTGAAAGA GCAGCTTGCC GAACTGAAGA AAAAGAACCA GATCTCTCAT 
CTGTCTGCTG AGAAGAACAT TCACCTGGAA AGACAGCTGG AGGAGGTGAG TGCCAAGCTT 
CAGGCCGAGC TGGAGGAGTC GGAGAGGCTG AAGAAGGCCC AGATCGAGGC CTTTAGACAG 
TCCCAGCAGC TGGAGCTTTC CCTCAGGGAG CTGCAGGAGC GTCTGGCCCA GCTGGAGAAC 
AGCAGGCTGG TTCTGGAGCA GGACAAACTC AGCCTGCAGA CCTCGCTAGA GCTGGAGAAA 
CGAGAGAGAA ACGCCGGCTC AGAGACCATC ACTGACCTGC AGGGTCGTAT CTATGGACTG 
GAGGGTGAGT TGAAGCATAT AAAGAGTTCT CTATCAAAAG CTGAAGTTGA GAAAAGACAA 
TTACAAGAGG ATCTTACAAC CCTGGAAAAG GAGAAGAATA AGCAGGAGAT CGACCTCTCC 
TTCAAACTCA AAGCAGTTCA GCAGAGTCTG GAGCAGGAGG AGGCTGAACA TAAAACCACT 
AAAGCCAGAT TGGCCGACAA CAACAAAATC AACCAATCCA TAGAGGCAAA GTCTGAGACC 
CTGAAAGACA TGGAACATAA GCTGTTGGAA GAACGATCTG CGAAGCAACA ACTGGAAAAC 
AGACTGATGC AGCTGGAGAA GGAAAACTCT GTATTAGACT GTGATTACAA ACAAGCCAAA 
CATGAGCTTC AGGAGCTCCG CTCGCTCAAA GAAAATCTCA CAGAGCAGGT GGAGGTGCTG 
AATGTGCGGG TGCAGCAGGA GACACAGAGG AAAACTCTCT GTCAAGGTGA TCTCAAAGTT 
CAGAGGCAGG AGATCAACAG CCTGCGGTCA TCAGAACAAC AGCTCAAACA AGAACTCAAC 
CATCTGCTGG AGCTCAAACT CACACTGGAG AAACAAAACC AGGAGCTGTC CAAGGAACGA 
GAAGAATCTG AAAAACAGCT GAAGGAGATG AAGGACCAGC TGGAGGCTGA GCAGTACTTC 
ACGAAACTGT ACAAGACTCA GATTCGAGAG CTGAAAGAGG AAAGCGATGA AAAGGTTAAA 
CTCTACAAAG ATGCTCAGCA GAGAATAGAG GACCTGCAGG AAGAGAGGGA CTCGTTAGCG 
TCTCAGTTGG AGGTGAGCCT GACGAAGGCT GACTCTGAAC AGTTGGCTCG CATCACCGTT 
GAAGAGCAAT ACTCGGACCT GGAGAAAGAG AAGATCATGA AGGAACTGGA GATAAAGGAC 
ATGATCGCCA GACACCGGCA GGATCTTGCA GAAAAGGACG GTACCATCAA CTCGCTGGAG 
GAGTCAAACC GTACGCTGAC TGTGGATGTT GCCAACCTGG CCAGTGAGAA AGAGGAGCTC 
AACAATAAAC TGAAGCACAT ACAGCAAAAG CTGGAGAAGA TCAAAGAAGA AGAGAAACAG 
ATGAAATCTC TCACAGTGTC GTATGAGAAG CAGATACAGG TGGAGAAAAC ACTCAAGATT 
CAGGCTATAA ACAAGCTGGC GGAGGTCATG AAGAAGACGG ACGGCAGGCC ACACCAAATC 
AACAACACAG ACATCCGCAG GAAGGAGAAG GAGATCAGAC AACTCCAGCT GCAGCTCCGC 
ACGGAGAAAG AAAAGCTTAA CCACTCCATC ATCAAATACC AGAGAGAAAT CAACGACATG 
CAGGCTATGA TAGCAGAGGA GTCTCAGGTG CGTCTGGAGA TGCAGATGTC TCTGGACAGT 
AAAGACAGTG ATATTGAGCG TCTGCGCAGT CAACTCACGT CTCTGAGCAT TCACTCTCTG 
GACACCACCA GCATCAGCAG CATCGGCAAT GACCTGGATT CTGACGAGGC ATATCCAGTG 
CGAATCACAC ACTCACACAC CTCGGAGTCT ATGTCCTTCA CATACCAGCG AAGCTCCAAG 
TCGGTGTGTA TTGATACCCG GCCCTGTCGA ATCGCTCCTC GCTTCTCCTC TGACTCTGAT 
GAAGAGGAGG ATGAGGAAGA TGAGTGGAGA CAAACACCTG CCCTTACATA TGAGACGCCT 
CCTGACCCTG AGGCTGCAGC CGCAGAGTCC GGTCTTGAAG GATGGCTCTC AATGCCTGCC 
AAAAACACCA AACGGTTTGG ATGGGATAAG AAGTATGTGG TTGTGAGCAG CAAGAAAATC 
CTGTTTTATG ACTCTGAACA AGACCGAGAG CAGTCCAGTC CATTCATGAT CCTGGACATT 
GACAAACTCT TCCACGTGAG ACCCGTCACT CAGACAGATG TTTACCGCGC AGATCCCAGA 
GAAATCCCTC GCATCTTCCA GATACTATAT GCGAATGAAG GAGAGAGCAA GAAAGAGGAG 
CTGCCCGTGG AGTCGTTATC TGTGACTGAG AGGTCACTGT GTATCCCACA CAAGGGTCAC 
GAGTTTGTTC TGACGCTTTA TCACTTCCCC TCGAGCTGCG AGGTCTGTCC ACGACCCTTA 
TGGCACGTCT TCAGACCTCC GCCGGCTCTC GAGTGCAGAC GCTGTAGAGT CAAGTGCCAT 
AAAGACCACA TCGATCGCAA AGAAGAGGTC CTCGCTCCAT GTAGAGTGAA TTACGACATG 
TCCACAGCGA AAGAGATGCT GCTGCTGGCA AACTCCACAG AGGAGCAGAA GAAGTGGGTC 
AGTCGGCTGC TCAAGAGGGT TCCGCGTAAA CCTTCCATCG CCCACTCTTC CAGCATAGCC 
ATATCCGCTG CGCCCTCAGA GGCAACACCC ACTACCCTTT CCCCTCAGCC GTCGCCTCGC 
CTCGCTCAGT CTTCACCCAG ACTTTCACAC CGAGCAGCCG TCAAAGTGCA CTCTACACGA 
CAGCAGCCCT CCAGCAAGAA CAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207922.1
CDS	474..4880
Translation	MSTGAGKRIESRVRKLETLIKNQKSPLNLESLLDSVTALALDLNHPALRKNKNIDAFLSRYEKAVCNLKELQVKLDDFDRVKLIGRGAFGAVQLVRHKVSQQVYAMKQLSKFEMVKRSDSAFFWEERDIMAFSQSPWIVQLCCAFQDEKYLYLVMEFMPGGDLVTLTSNYDIPEEWAQFYTAEVVLALDAIHSLGFIHRDIKPDNMLLDRNGHLKLADFGTCMKMDSTGMVRCDTAVGTPDYISPEVLMSQGGTGYYGRECDWWSVGVFIYELLVGDTPFYSESLVGTYGKIMDHKNSLTFPDDIEMSKNAKDLICAFLSSREVRLGRTGVDEIKCHPFFKNDQWTFDTIRDTMAPVVPELSSDIDTSNFDDDIKDDPIGTETFPPPRAFAGNQLPFVGFTYFREDQLLTQQNKCSEEDIIEDHGLNHTESNDLEKRLKKLEEKFKHEMQAKEELENKCRIANQRLEKLSKDLEEEVNARQEAEDNLRSLEKEKVLLKHQRTQSVRKAGLETDRKRLLENEVSSLKEQLAELKKKNQISHLSAEKNIHLERQLEEVSAKLQAELEESERLKKAQIEAFRQSQQLELSLRELQERLAQLENSRLVLEQDKLSLQTSLELEKRERNAGSETITDLQGRIYGLEGELKHIKSSLSKAEVEKRQLQEDLTTLEKEKNKQEIDLSFKLKAVQQSLEQEEAEHKTTKARLADNNKINQSIEAKSETLKDMEHKLLEERSAKQQLENRLMQLEKENSVLDCDYKQAKHELQELRSLKENLTEQVEVLNVRVQQETQRKTLCQGDLKVQRQEINSLRSSEQQLKQELNHLLELKLTLEKQNQELSKEREESEKQLKEMKDQLEAEQYFTKLYKTQIRELKEESDEKVKLYKDAQQRIEDLQEERDSLASQLEVSLTKADSEQLARITVEEQYSDLEKEKIMKELEIKDMIARHRQDLAEKDGTINSLEESNRTLTVDVANLASEKEELNNKLKHIQQKLEKIKEEEKQMKSLTVSYEKQIQVEKTLKIQAINKLAEVMKKTDGRPHQINNTDIRRKEKEIRQLQLQLRTEKEKLNHSIIKYQREINDMQAMIAEESQVRLEMQMSLDSKDSDIERLRSQLTSLSIHSLDTTSISSIGNDLDSDEAYPVRITHSHTSESMSFTYQRSSKSVCIDTRPCRIAPRFSSDSDEEEDEEDEWRQTPALTYETPPDPEAAAAESGLEGWLSMPAKNTKRFGWDKKYVVVSSKKILFYDSEQDREQSSPFMILDIDKLFHVRPVTQTDVYRADPREIPRIFQILYANEGESKKEELPVESLSVTERSLCIPHKGHEFVLTLYHFPSSCEVCPRPLWHVFRPPPALECRRCRVKCHKDHIDRKEEVLAPCRVNYDMSTAKEMLLLANSTEEQKKWVSRLLKRVPRKPSIAHSSSIAISAAPSEATPTTLSPQPSPRLAQSSPRLSHRAAVKVHSTRQQPSSKNS

Target ORF information:

RefSeq Version	XM_017352433.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352433.2

ORF Insert Sequence:

ATGTCGACCG GAGCGGGGAA GAGAATAGAG AGCCGGGTGA GAAAACTAGA GACGCTGATC 
AAGAACCAGA AATCACCGTT GAACTTGGAG AGTTTGCTGG ATTCAGTAAC TGCCCTTGCC 
CTTGATCTGA ACCATCCAGC ACTGAGAAAG AACAAAAACA TAGATGCCTT CTTAAGCAGA 
TATGAAAAAG CCGTGTGCAA CCTCAAGGAA CTACAGGTGA AGCTGGATGA TTTTGATCGA 
GTCAAGCTGA TCGGTCGTGG AGCGTTTGGC GCAGTGCAGC TGGTACGGCA CAAAGTATCT 
CAACAAGTTT ATGCCATGAA GCAGCTCAGC AAGTTTGAGA TGGTCAAGCG CTCTGACTCC 
GCCTTTTTCT GGGAGGAGCG TGACATCATG GCTTTCTCCC AGAGCCCTTG GATAGTTCAG 
CTGTGCTGTG CATTCCAAGA TGAAAAGTAC CTTTATTTGG TGATGGAGTT CATGCCAGGT 
GGAGATCTGG TGACTCTGAC CAGCAACTAT GACATCCCAG AGGAATGGGC TCAATTCTAT 
ACGGCAGAAG TGGTGCTGGC ACTCGACGCC ATCCATTCTC TGGGCTTCAT CCACAGAGAC 
ATCAAACCAG ACAACATGCT GCTGGACCGC AATGGACACC TCAAACTCGC AGACTTTGGC 
ACATGCATGA AGATGGACTC GACTGGTATG GTACGCTGTG ACACTGCTGT TGGGACACCT 
GATTACATTT CTCCAGAGGT TTTAATGTCT CAAGGAGGGA CTGGTTATTA CGGTCGCGAG 
TGTGACTGGT GGTCTGTTGG TGTTTTTATT TATGAGCTTC TAGTGGGCGA CACTCCATTT 
TATTCCGAGT CTCTGGTGGG CACATATGGA AAGATCATGG ATCACAAGAA CAGCTTAACT 
TTCCCAGATG ATATTGAAAT GTCAAAGAAT GCCAAAGACC TCATCTGTGC CTTTCTGAGT 
AGCAGGGAGG TGAGGCTTGG CCGAACTGGA GTGGATGAAA TAAAATGTCA CCCCTTCTTT 
AAGAATGATC AGTGGACCTT CGACACCATA CGAGACACCA TGGCTCCAGT GGTGCCAGAG 
CTGAGTAGCG ATATTGACAC CAGTAACTTT GATGATGACA TTAAGGATGA TCCGATAGGA 
ACAGAGACGT TCCCTCCGCC CCGTGCCTTC GCAGGGAACC AGCTACCATT TGTGGGTTTC 
ACCTATTTCA GGGAGGACCA ATTGTTAACA CAACAAAATA AGTGTTCAGA GGAAGATATC 
ATTGAAGATC ATGGCTTAAA CCACACAGAG TCTAACGACC TGGAGAAGAG GCTGAAAAAG 
TTGGAGGAAA AATTCAAACA TGAGATGCAA GCAAAGGAAG AACTGGAAAA TAAATGCCGA 
ATAGCAAATC AGCGGCTGGA GAAATTGTCC AAAGATCTTG AAGAAGAAGT GAATGCGAGA 
CAGGAGGCGG AAGACAACCT GAGATCCCTG GAGAAAGAGA AAGTTTTACT GAAACACCAG 
CGTACACAGA GCGTAAGAAA AGCTGGTCTA GAGACCGACA GAAAACGGCT GCTGGAGAAT 
GAAGTAAGCA GCCTGAAAGA GCAGCTTGCC GAACTGAAGA AAAAGAACCA GATCTCTCAT 
CTGTCTGCTG AGAAGAACAT TCACCTGGAA AGACAGCTGG AGGAGGTGAG TGCCAAGCTT 
CAGGCCGAGC TGGAGGAGTC GGAGAGGCTG AAGAAGGCCC AGATCGAGGC CTTTAGACAG 
TCCCAGCAGC TGGAGCTTTC CCTCAGGGAG CTGCAGGAGC GTCTGGCCCA GCTGGAGAAC 
AGCAGGCTGG TTCTGGAGCA GGACAAACTC AGCCTGCAGA CCTCGCTAGA GCTGGAGAAA 
CGAGAGAGAA ACGCCGGCTC AGAGACCATC ACTGACCTGC AGGGTCGTAT CTATGGACTG 
GAGGGTGAGT TGAAGCATAT AAAGAGTTCT CTATCAAAAG CTGAAGTTGA GAAAAGACAA 
TTACAAGAGG ATCTTACAAC CCTGGAAAAG GAGAAGAATA AGCAGGAGAT CGACCTCTCC 
TTCAAACTCA AAGCAGTTCA GCAGAGTCTG GAGCAGGAGG AGGCTGAACA TAAAACCACT 
AAAGCCAGAT TGGCCGACAA CAACAAAATC AACCAATCCA TAGAGGCAAA GTCTGAGACC 
CTGAAAGACA TGGAACATAA GCTGTTGGAA GAACGATCTG CGAAGCAACA ACTGGAAAAC 
AGACTGATGC AGCTGGAGAA GGAAAACTCT GTATTAGACT GTGATTACAA ACAAGCCAAA 
CATGAGCTTC AGGAGCTCCG CTCGCTCAAA GAAAATCTCA CAGAGCAGGT GGAGGTGCTG 
AATGTGCGGG TGCAGCAGGA GACACAGAGG AAAACTCTCT GTCAAGGTGA TCTCAAAGTT 
CAGAGGCAGG AGATCAACAG CCTGCGGTCA TCAGAACAAC AGCTCAAACA AGAACTCAAC 
CATCTGCTGG AGCTCAAACT CACACTGGAG AAACAAAACC AGGAGCTGTC CAAGGAACGA 
GAAGAATCTG AAAAACAGCT GAAGGAGATG AAGGACCAGC TGGAGGCTGA GCAGTACTTC 
ACGAAACTGT ACAAGACTCA GATTCGAGAG CTGAAAGAGG AAAGCGATGA AAAGGTTAAA 
CTCTACAAAG ATGCTCAGCA GAGAATAGAG GACCTGCAGG AAGAGAGGGA CTCGTTAGCG 
TCTCAGTTGG AGGTGAGCCT GACGAAGGCT GACTCTGAAC AGTTGGCTCG CATCACCGTT 
GAAGAGCAAT ACTCGGACCT GGAGAAAGAG AAGATCATGA AGGAACTGGA GATAAAGGAC 
ATGATCGCCA GACACCGGCA GGATCTTGCA GAAAAGGACG GTACCATCAA CTCGCTGGAG 
GAGTCAAACC GTACGCTGAC TGTGGATGTT GCCAACCTGG CCAGTGAGAA AGAGGAGCTC 
AACAATAAAC TGAAGCACAT ACAGCAAAAG CTGGAGAAGA TCAAAGAAGA AGAGAAACAG 
ATGAAATCTC TCACAGTGTC GTATGAGAAG CAGATACAGG TGGAGAAAAC ACTCAAGATT 
CAGGCTATAA ACAAGCTGGC GGAGGTCATG AAGAAGACGG ACGGCAGGCC ACACCAAATC 
AACAACACAG ACATCCGCAG GAAGGAGAAG GAGATCAGAC AACTCCAGCT GCAGCTCCGC 
ACGGAGAAAG AAAAGCTTAA CCACTCCATC ATCAAATACC AGAGAGAAAT CAACGACATG 
CAGGCTATGA TAGCAGAGGA GTCTCAGGTG CGTCTGGAGA TGCAGATGTC TCTGGACAGT 
AAAGACAGTG ATATTGAGCG TCTGCGCAGT CAACTCACGT CTCTGAGCAT TCACTCTCTG 
GACACCACCA GCATCAGCAG CATCGGCAAT GACCTGGATT CTGACGAGGC ATATCCAGTG 
CGAATCACAC ACTCACACAC CTCGGAGTCT ATGTCCTTCA CATACCAGCG AAGCTCCAAG 
TCGGTGTGTA TTGATACCCG GCCCTGTCGA ATCGCTCCTC GCTTCTCCTC TGACTCTGAT 
GAAGAGGAGG ATGAGGAAGA TGAGTGGAGA CAAACACCTG CCCTTACATA TGAGACGCCT 
CCTGACCCTG AGGCTGCAGC CGCAGAGTCC GGTCTTGAAG GATGGCTCTC AATGCCTGCC 
AAAAACACCA AACGGTTTGG ATGGGATAAG AAGTATGTGG TTGTGAGCAG CAAGAAAATC 
CTGTTTTATG ACTCTGAACA AGACCGAGAG CAGTCCAGTC CATTCATGAT CCTGGACATT 
GACAAACTCT TCCACGTGAG ACCCGTCACT CAGACAGATG TTTACCGCGC AGATCCCAGA 
GAAATCCCTC GCATCTTCCA GATACTATAT GCGAATGAAG GAGAGAGCAA GAAAGAGGAG 
CTGCCCGTGG AGTCGTTATC TGTGACTGAG AGGTCACTGT GTATCCCACA CAAGGGTCAC 
GAGTTTGTTC TGACGCTTTA TCACTTCCCC TCGAGCTGCG AGGTCTGTCC ACGACCCTTA 
TGGCACGTCT TCAGACCTCC GCCGGCTCTC GAGTGCAGAC GCTGTAGAGT CAAGTGCCAT 
AAAGACCACA TCGATCGCAA AGAAGAGGTC CTCGCTCCAT GTAGAGTGAA TTACGACATG 
TCCACAGCGA AAGAGATGCT GCTGCTGGCA AACTCCACAG AGGAGCAGAA GAAGTGGGTC 
AGTCGGCTGC TCAAGAGGGT TCCGCGTAAA CCTTCCATCG CCCACTCTTC CAGCATAGCC 
ATATCCGCTG CGCCCTCAGA GGCAACACCC ACTACCCTTT CCCCTCAGCC GTCGCCTCGC 
CTCGCTCAGT CTTCACCCAG ACTTTCACAC CGAGCAGCCG TCAAAGTGCA CTCTACACGA 
CAGCAGCCCT CCAGCAAGAA CAGCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rock2b ( XM_017352434.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207923.1 Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa39641
Clone ID Related Accession (Same CDS sequence)	XM_017352434.2 , XM_017352434.1
Accession Version	XM_017352434.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho-associated, coiled-coil containing protein kinase 2b isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017352434.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGACCG GAGCGGGGAA GAGAATAGAG AGCCGGGTGA GAAAACTAGA GACGCTGATC 
AAGAACCAGA AATCACCGTT GAACTTGGAG AGTTTGCTGG ATTCAGTAAC TGCCCTTGCC 
CTTGATCTGA ACCATCCAGC ACTGAGAAAG AACAAAAACA TAGATGCCTT CTTAAGCAGA 
TATGAAAAAG CCGTGTGCAA CCTCAAGGAA CTACAGGTGA AGCTGGATGA TTTTGATCGA 
GTCAAGCTGA TCGGTCGTGG AGCGTTTGGC GCAGTGCAGC TGGTACGGCA CAAAGTATCT 
CAACAAGTTT ATGCCATGAA GCAGCTCAGC AAGTTTGAGA TGGTCAAGCG CTCTGACTCC 
GCCTTTTTCT GGGAGGAGCG TGACATCATG GCTTTCTCCC AGAGCCCTTG GATAGTTCAG 
CTGTGCTGTG CATTCCAAGA TGAAAAGTAC CTTTATTTGG TGATGGAGTT CATGCCAGGT 
GGAGATCTGG TGACTCTGAC CAGCAACTAT GACATCCCAG AGGAATGGGC TCAATTCTAT 
ACGGCAGAAG TGGTGCTGGC ACTCGACGCC ATCCATTCTC TGGGCTTCAT CCACAGAGAC 
ATCAAACCAG ACAACATGCT GCTGGACCGC AATGGACACC TCAAACTCGC AGACTTTGGC 
ACATGCATGA AGATGGACTC GACTGGTATG GTACGCTGTG ACACTGCTGT TGGGACACCT 
GATTACATTT CTCCAGAGGT TTTAATGTCT CAAGGAGGGA CTGGTTATTA CGGTCGCGAG 
TGTGACTGGT GGTCTGTTGG TGTTTTTATT TATGAGCTTC TAGTGGGCGA CACTCCATTT 
TATTCCGAGT CTCTGGTGGG CACATATGGA AAGATCATGG ATCACAAGAA CAGCTTAACT 
TTCCCAGATG ATATTGAAAT GTCAAAGAAT GCCAAAGACC TCATCTGTGC CTTTCTGAGT 
AGCAGGGAGG TGAGGCTTGG CCGAACTGGA GTGGATGAAA TAAAATGTCA CCCCTTCTTT 
AAGAATGATC AGTGGACCTT CGACACCATA CGAGACACCA TGGCTCCAGT GGTGCCAGAG 
CTGAGTAGCG ATATTGACAC CAGTAACTTT GATGATGACA TTAAGGATGA TCCGATAGGA 
ACAGAGACGT TCCCTCCGCC CCGTGCCTTC GCAGGGAACC AGCTACCATT TGTGGGTTTC 
ACCTATTTCA GGGAGGACCA ATTGTTAACA CAACAAAATA AGTGTTCAGA GGAAGATATC 
ATTGAAGATC ATGGCTTAAA CCACACAGAG TCTAACGACC TGGAGAAGAG GCTGAAAAAG 
TTGGAGGAAA AATTCAAACA TGAGATGCAA GCAAAGGAAG AACTGGAAAA TAAATGCCGA 
ATAGCAAATC AGCGGCTGGA GAAATTGTCC AAAGATCTTG AAGAAGAAGT GAATGCGAGA 
CAGGAGGCGG AAGACAACCT GAGATCCCTG GAGAAAGAGA AAGTTTTACT GAAACACCAG 
CGTACACAGA GCGTAAGAAA AGCTGGTCTA GAGACCGACA GAAAACGGCT GCTGGAGAAT 
GAAGTAAGCA GCCTGAAAGA GCAGCTTGCC GAACTGAAGA AAAAGAACCA GATCTCTCAT 
CTGTCTGCTG AGAAGAACAT TCACCTGGAA AGACAGCTGG AGGAGGTGAG TGCCAAGCTT 
CAGGCCGAGC TGGAGGAGTC GGAGAGGCTG AAGAAGGCCC AGATCGAGGC CTTTAGACAG 
TCCCAGCAGC TGGAGCTTTC CCTCAGGGAG CTGCAGGAGC GTCTGGCCCA GCTGGAGAAC 
AGCAGGCTGG TTCTGGAGCA GGACAAACTC AGCCTGCAGA CCTCGCTAGA GCTGGAGAAA 
CGAGAGAGAA ACGCCGGCTC AGAGACCATC ACTGACCTGC AGGGTCGTAT CTATGGACTG 
GAGGGTGAGT TGAAGCATAT AAAGAGTTCT CTATCAAAAG CTGAAGTTGA GAAAAGACAA 
TTACAAGAGG ATCTTACAAC CCTGGAAAAG GAGAAGAATA AGCAGGAGAT CGACCTCTCC 
TTCAAACTCA AAGCAGTTCA GCAGAGTCTG GAGCAGGAGG AGGCTGAACA TAAAACCACT 
AAAGCCAGAT TGGCCGACAA CAACAAAATC AACCAATCCA TAGAGGCAAA GTCTGAGACC 
CTGAAAGACA TGGAACATAA GCTGTTGGAA GAACGATCTG CGAAGCAACA ACTGGAAAAC 
AGACTGATGC AGCTGGAGAA GGAAAACTCT GTATTAGACT GTGATTACAA ACAAGCCAAA 
CATGAGCTTC AGGAGCTCCG CTCGCTCAAA GAAAATCTCA CAGAGCAGGT GGAGGTGCTG 
AATGTGCGGG TGCAGCAGGA GACACAGAGG AAAACTCTCT GTCAAGGTGA TCTCAAAGTT 
CAGAGGCAGG AGATCAACAG CCTGCGGTCA TCAGAACAAC AGCTCAAACA AGAACTCAAC 
CATCTGCTGG AGCTCAAACT CACACTGGAG AAACAAAACC AGGAGCTGTC CAAGGAACGA 
GAAGAATCTG AAAAACAGCT GAAGGAGATG AAGGACCAGC TGGAGGCTGA GCAGTACTTC 
ACGAAACTGT ACAAGACTCA GATTCGAGAG CTGAAAGAGG AAAGCGATGA AAAGGTTAAA 
CTCTACAAAG ATGCTCAGCA GAGAATAGAG GACCTGCAGG AAGAGAGGGA CTCGTTAGCG 
TCTCAGTTGG AGGTGAGCCT GACGAAGGCT GACTCTGAAC AGTTGGCTCG CATCACCGTT 
GAAGAGCAAT ACTCGGACCT GGAGAAAGAG AAGATCATGA AGGAACTGGA GATAAAGGAC 
ATGATCGCCA GACACCGGCA GGATCTTGCA GAAAAGGACG GTACCATCAA CTCGCTGGAG 
GAGTCAAACC GTACGCTGAC TGTGGATGTT GCCAACCTGG CCAGTGAGAA AGAGGAGCTC 
AACAATAAAC TGAAGCACAT ACAGCAAAAG CTGGAGAAGA TCAAAGAAGA AGAGAAACAG 
ATGAAATCTC TCACAGTGTC GTATGAGAAG CAGATACAGG TGGAGAAAAC ACTCAAGATT 
CAGGCTATAA ACAAGCTGGC GGAGGTCATG AAGAAGACGG ACGGCAGGCC ACACCAAATC 
AACAACACAG ACATCCGCAG GAAGGAGAAG GAGATCAGAC AACTCCAGCT GCAGCTCCGC 
ACGGAGAAAG AAAAGCTTAA CCACTCCATC ATCAAATACC AGAGAGAAAT CAACGACATG 
CAGGCTATGA TAGCAGAGGA GTCTCAGGTG CGTCTGGAGA TGCAGATGTC TCTGGACAGT 
AAAGACAGTG ATATTGAGCG TCTGCGCAGT CAACTCACGT CTCTGAGCAT TCACTCTCTG 
GACACCACCA GCATCAGCAG CATCGGCAAT GACCTGGATT CTGACGAGGC ATATCCAGCC 
GCAGAGTCCG GTCTTGAAGG ATGGCTCTCA ATGCCTGCCA AAAACACCAA ACGGTTTGGA 
TGGGATAAGA AGTATGTGGT TGTGAGCAGC AAGAAAATCC TGTTTTATGA CTCTGAACAA 
GACCGAGAGC AGTCCAGTCC ATTCATGATC CTGGACATTG ACAAACTCTT CCACGTGAGA 
CCCGTCACTC AGACAGATGT TTACCGCGCA GATCCCAGAG AAATCCCTCG CATCTTCCAG 
ATACTATATG CGAATGAAGG AGAGAGCAAG AAAGAGGAGC TGCCCGTGGA GTCGTTATCT 
GTGACTGAGA GGTCACTGTG TATCCCACAC AAGGGTCACG AGTTTGTTCT GACGCTTTAT 
CACTTCCCCT CGAGCTGCGA GGTCTGTCCA CGACCCTTAT GGCACGTCTT CAGACCTCCG 
CCGGCTCTCG AGTGCAGACG CTGTAGAGTC AAGTGCCATA AAGACCACAT CGATCGCAAA 
GAAGAGGTCC TCGCTCCATG TAGAGTGAAT TACGACATGT CCACAGCGAA AGAGATGCTG 
CTGCTGGCAA ACTCCACAGA GGAGCAGAAG AAGTGGGTCA GTCGGCTGCT CAAGAGGGTT 
CCGCGTAAAC CTTCCATCGC CCACTCTTCC AGCATAGCCA TATCCGCTGC GCCCTCAGAG 
GCAACACCCA CTACCCTTTC CCCTCAGCCG TCGCCTCGCC TCGCTCAGTC TTCACCCAGA 
CTTTCACACC GAGCAGCCGT CAAAGTGCAC TCTACACGAC AGCAGCCCTC CAGCAAGAAC 
AGATTGCTTG AAATGGGCCT GAAGGACTGG GGTTGGGCTC TTGAGGTTGA ATTGGATGAT 
GACGATGTTT TAGACTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207923.1
CDS	474..4754
Translation	MSTGAGKRIESRVRKLETLIKNQKSPLNLESLLDSVTALALDLNHPALRKNKNIDAFLSRYEKAVCNLKELQVKLDDFDRVKLIGRGAFGAVQLVRHKVSQQVYAMKQLSKFEMVKRSDSAFFWEERDIMAFSQSPWIVQLCCAFQDEKYLYLVMEFMPGGDLVTLTSNYDIPEEWAQFYTAEVVLALDAIHSLGFIHRDIKPDNMLLDRNGHLKLADFGTCMKMDSTGMVRCDTAVGTPDYISPEVLMSQGGTGYYGRECDWWSVGVFIYELLVGDTPFYSESLVGTYGKIMDHKNSLTFPDDIEMSKNAKDLICAFLSSREVRLGRTGVDEIKCHPFFKNDQWTFDTIRDTMAPVVPELSSDIDTSNFDDDIKDDPIGTETFPPPRAFAGNQLPFVGFTYFREDQLLTQQNKCSEEDIIEDHGLNHTESNDLEKRLKKLEEKFKHEMQAKEELENKCRIANQRLEKLSKDLEEEVNARQEAEDNLRSLEKEKVLLKHQRTQSVRKAGLETDRKRLLENEVSSLKEQLAELKKKNQISHLSAEKNIHLERQLEEVSAKLQAELEESERLKKAQIEAFRQSQQLELSLRELQERLAQLENSRLVLEQDKLSLQTSLELEKRERNAGSETITDLQGRIYGLEGELKHIKSSLSKAEVEKRQLQEDLTTLEKEKNKQEIDLSFKLKAVQQSLEQEEAEHKTTKARLADNNKINQSIEAKSETLKDMEHKLLEERSAKQQLENRLMQLEKENSVLDCDYKQAKHELQELRSLKENLTEQVEVLNVRVQQETQRKTLCQGDLKVQRQEINSLRSSEQQLKQELNHLLELKLTLEKQNQELSKEREESEKQLKEMKDQLEAEQYFTKLYKTQIRELKEESDEKVKLYKDAQQRIEDLQEERDSLASQLEVSLTKADSEQLARITVEEQYSDLEKEKIMKELEIKDMIARHRQDLAEKDGTINSLEESNRTLTVDVANLASEKEELNNKLKHIQQKLEKIKEEEKQMKSLTVSYEKQIQVEKTLKIQAINKLAEVMKKTDGRPHQINNTDIRRKEKEIRQLQLQLRTEKEKLNHSIIKYQREINDMQAMIAEESQVRLEMQMSLDSKDSDIERLRSQLTSLSIHSLDTTSISSIGNDLDSDEAYPAAESGLEGWLSMPAKNTKRFGWDKKYVVVSSKKILFYDSEQDREQSSPFMILDIDKLFHVRPVTQTDVYRADPREIPRIFQILYANEGESKKEELPVESLSVTERSLCIPHKGHEFVLTLYHFPSSCEVCPRPLWHVFRPPPALECRRCRVKCHKDHIDRKEEVLAPCRVNYDMSTAKEMLLLANSTEEQKKWVSRLLKRVPRKPSIAHSSSIAISAAPSEATPTTLSPQPSPRLAQSSPRLSHRAAVKVHSTRQQPSSKNRLLEMGLKDWGWALEVELDDDDVLDF

Target ORF information:

RefSeq Version	XM_017352434.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352434.2

ORF Insert Sequence:

ATGTCGACCG GAGCGGGGAA GAGAATAGAG AGCCGGGTGA GAAAACTAGA GACGCTGATC 
AAGAACCAGA AATCACCGTT GAACTTGGAG AGTTTGCTGG ATTCAGTAAC TGCCCTTGCC 
CTTGATCTGA ACCATCCAGC ACTGAGAAAG AACAAAAACA TAGATGCCTT CTTAAGCAGA 
TATGAAAAAG CCGTGTGCAA CCTCAAGGAA CTACAGGTGA AGCTGGATGA TTTTGATCGA 
GTCAAGCTGA TCGGTCGTGG AGCGTTTGGC GCAGTGCAGC TGGTACGGCA CAAAGTATCT 
CAACAAGTTT ATGCCATGAA GCAGCTCAGC AAGTTTGAGA TGGTCAAGCG CTCTGACTCC 
GCCTTTTTCT GGGAGGAGCG TGACATCATG GCTTTCTCCC AGAGCCCTTG GATAGTTCAG 
CTGTGCTGTG CATTCCAAGA TGAAAAGTAC CTTTATTTGG TGATGGAGTT CATGCCAGGT 
GGAGATCTGG TGACTCTGAC CAGCAACTAT GACATCCCAG AGGAATGGGC TCAATTCTAT 
ACGGCAGAAG TGGTGCTGGC ACTCGACGCC ATCCATTCTC TGGGCTTCAT CCACAGAGAC 
ATCAAACCAG ACAACATGCT GCTGGACCGC AATGGACACC TCAAACTCGC AGACTTTGGC 
ACATGCATGA AGATGGACTC GACTGGTATG GTACGCTGTG ACACTGCTGT TGGGACACCT 
GATTACATTT CTCCAGAGGT TTTAATGTCT CAAGGAGGGA CTGGTTATTA CGGTCGCGAG 
TGTGACTGGT GGTCTGTTGG TGTTTTTATT TATGAGCTTC TAGTGGGCGA CACTCCATTT 
TATTCCGAGT CTCTGGTGGG CACATATGGA AAGATCATGG ATCACAAGAA CAGCTTAACT 
TTCCCAGATG ATATTGAAAT GTCAAAGAAT GCCAAAGACC TCATCTGTGC CTTTCTGAGT 
AGCAGGGAGG TGAGGCTTGG CCGAACTGGA GTGGATGAAA TAAAATGTCA CCCCTTCTTT 
AAGAATGATC AGTGGACCTT CGACACCATA CGAGACACCA TGGCTCCAGT GGTGCCAGAG 
CTGAGTAGCG ATATTGACAC CAGTAACTTT GATGATGACA TTAAGGATGA TCCGATAGGA 
ACAGAGACGT TCCCTCCGCC CCGTGCCTTC GCAGGGAACC AGCTACCATT TGTGGGTTTC 
ACCTATTTCA GGGAGGACCA ATTGTTAACA CAACAAAATA AGTGTTCAGA GGAAGATATC 
ATTGAAGATC ATGGCTTAAA CCACACAGAG TCTAACGACC TGGAGAAGAG GCTGAAAAAG 
TTGGAGGAAA AATTCAAACA TGAGATGCAA GCAAAGGAAG AACTGGAAAA TAAATGCCGA 
ATAGCAAATC AGCGGCTGGA GAAATTGTCC AAAGATCTTG AAGAAGAAGT GAATGCGAGA 
CAGGAGGCGG AAGACAACCT GAGATCCCTG GAGAAAGAGA AAGTTTTACT GAAACACCAG 
CGTACACAGA GCGTAAGAAA AGCTGGTCTA GAGACCGACA GAAAACGGCT GCTGGAGAAT 
GAAGTAAGCA GCCTGAAAGA GCAGCTTGCC GAACTGAAGA AAAAGAACCA GATCTCTCAT 
CTGTCTGCTG AGAAGAACAT TCACCTGGAA AGACAGCTGG AGGAGGTGAG TGCCAAGCTT 
CAGGCCGAGC TGGAGGAGTC GGAGAGGCTG AAGAAGGCCC AGATCGAGGC CTTTAGACAG 
TCCCAGCAGC TGGAGCTTTC CCTCAGGGAG CTGCAGGAGC GTCTGGCCCA GCTGGAGAAC 
AGCAGGCTGG TTCTGGAGCA GGACAAACTC AGCCTGCAGA CCTCGCTAGA GCTGGAGAAA 
CGAGAGAGAA ACGCCGGCTC AGAGACCATC ACTGACCTGC AGGGTCGTAT CTATGGACTG 
GAGGGTGAGT TGAAGCATAT AAAGAGTTCT CTATCAAAAG CTGAAGTTGA GAAAAGACAA 
TTACAAGAGG ATCTTACAAC CCTGGAAAAG GAGAAGAATA AGCAGGAGAT CGACCTCTCC 
TTCAAACTCA AAGCAGTTCA GCAGAGTCTG GAGCAGGAGG AGGCTGAACA TAAAACCACT 
AAAGCCAGAT TGGCCGACAA CAACAAAATC AACCAATCCA TAGAGGCAAA GTCTGAGACC 
CTGAAAGACA TGGAACATAA GCTGTTGGAA GAACGATCTG CGAAGCAACA ACTGGAAAAC 
AGACTGATGC AGCTGGAGAA GGAAAACTCT GTATTAGACT GTGATTACAA ACAAGCCAAA 
CATGAGCTTC AGGAGCTCCG CTCGCTCAAA GAAAATCTCA CAGAGCAGGT GGAGGTGCTG 
AATGTGCGGG TGCAGCAGGA GACACAGAGG AAAACTCTCT GTCAAGGTGA TCTCAAAGTT 
CAGAGGCAGG AGATCAACAG CCTGCGGTCA TCAGAACAAC AGCTCAAACA AGAACTCAAC 
CATCTGCTGG AGCTCAAACT CACACTGGAG AAACAAAACC AGGAGCTGTC CAAGGAACGA 
GAAGAATCTG AAAAACAGCT GAAGGAGATG AAGGACCAGC TGGAGGCTGA GCAGTACTTC 
ACGAAACTGT ACAAGACTCA GATTCGAGAG CTGAAAGAGG AAAGCGATGA AAAGGTTAAA 
CTCTACAAAG ATGCTCAGCA GAGAATAGAG GACCTGCAGG AAGAGAGGGA CTCGTTAGCG 
TCTCAGTTGG AGGTGAGCCT GACGAAGGCT GACTCTGAAC AGTTGGCTCG CATCACCGTT 
GAAGAGCAAT ACTCGGACCT GGAGAAAGAG AAGATCATGA AGGAACTGGA GATAAAGGAC 
ATGATCGCCA GACACCGGCA GGATCTTGCA GAAAAGGACG GTACCATCAA CTCGCTGGAG 
GAGTCAAACC GTACGCTGAC TGTGGATGTT GCCAACCTGG CCAGTGAGAA AGAGGAGCTC 
AACAATAAAC TGAAGCACAT ACAGCAAAAG CTGGAGAAGA TCAAAGAAGA AGAGAAACAG 
ATGAAATCTC TCACAGTGTC GTATGAGAAG CAGATACAGG TGGAGAAAAC ACTCAAGATT 
CAGGCTATAA ACAAGCTGGC GGAGGTCATG AAGAAGACGG ACGGCAGGCC ACACCAAATC 
AACAACACAG ACATCCGCAG GAAGGAGAAG GAGATCAGAC AACTCCAGCT GCAGCTCCGC 
ACGGAGAAAG AAAAGCTTAA CCACTCCATC ATCAAATACC AGAGAGAAAT CAACGACATG 
CAGGCTATGA TAGCAGAGGA GTCTCAGGTG CGTCTGGAGA TGCAGATGTC TCTGGACAGT 
AAAGACAGTG ATATTGAGCG TCTGCGCAGT CAACTCACGT CTCTGAGCAT TCACTCTCTG 
GACACCACCA GCATCAGCAG CATCGGCAAT GACCTGGATT CTGACGAGGC ATATCCAGCC 
GCAGAGTCCG GTCTTGAAGG ATGGCTCTCA ATGCCTGCCA AAAACACCAA ACGGTTTGGA 
TGGGATAAGA AGTATGTGGT TGTGAGCAGC AAGAAAATCC TGTTTTATGA CTCTGAACAA 
GACCGAGAGC AGTCCAGTCC ATTCATGATC CTGGACATTG ACAAACTCTT CCACGTGAGA 
CCCGTCACTC AGACAGATGT TTACCGCGCA GATCCCAGAG AAATCCCTCG CATCTTCCAG 
ATACTATATG CGAATGAAGG AGAGAGCAAG AAAGAGGAGC TGCCCGTGGA GTCGTTATCT 
GTGACTGAGA GGTCACTGTG TATCCCACAC AAGGGTCACG AGTTTGTTCT GACGCTTTAT 
CACTTCCCCT CGAGCTGCGA GGTCTGTCCA CGACCCTTAT GGCACGTCTT CAGACCTCCG 
CCGGCTCTCG AGTGCAGACG CTGTAGAGTC AAGTGCCATA AAGACCACAT CGATCGCAAA 
GAAGAGGTCC TCGCTCCATG TAGAGTGAAT TACGACATGT CCACAGCGAA AGAGATGCTG 
CTGCTGGCAA ACTCCACAGA GGAGCAGAAG AAGTGGGTCA GTCGGCTGCT CAAGAGGGTT 
CCGCGTAAAC CTTCCATCGC CCACTCTTCC AGCATAGCCA TATCCGCTGC GCCCTCAGAG 
GCAACACCCA CTACCCTTTC CCCTCAGCCG TCGCCTCGCC TCGCTCAGTC TTCACCCAGA 
CTTTCACACC GAGCAGCCGT CAAAGTGCAC TCTACACGAC AGCAGCCCTC CAGCAAGAAC 
AGATTGCTTG AAATGGGCCT GAAGGACTGG GGTTGGGCTC TTGAGGTTGA ATTGGATGAT 
GACGATGTTT TAGACTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rock2b ( XM_017352434.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017207923.1 Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa39641
Clone ID Related Accession (Same CDS sequence)	XM_017352434.2 , XM_017352434.1
Accession Version	XM_017352434.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4281bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	rho-associated, coiled-coil containing protein kinase 2b isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGACCG GAGCGGGGAA GAGAATAGAG AGCCGGGTGA GAAAACTAGA GACGCTGATC 
AAGAACCAGA AATCACCGTT GAACTTGGAG AGTTTGCTGG ATTCAGTAAC TGCCCTTGCC 
CTTGATCTGA ACCATCCAGC ACTGAGAAAG AACAAAAACA TAGATGCCTT CTTAAGCAGA 
TATGAAAAAG CCGTGTGCAA CCTCAAGGAA CTACAGGTGA AGCTGGATGA TTTTGATCGA 
GTCAAGCTGA TCGGTCGTGG AGCGTTTGGC GCAGTGCAGC TGGTACGGCA CAAAGTATCT 
CAACAAGTTT ATGCCATGAA GCAGCTCAGC AAGTTTGAGA TGGTCAAGCG CTCTGACTCC 
GCCTTTTTCT GGGAGGAGCG TGACATCATG GCTTTCTCCC AGAGCCCTTG GATAGTTCAG 
CTGTGCTGTG CATTCCAAGA TGAAAAGTAC CTTTATTTGG TGATGGAGTT CATGCCAGGT 
GGAGATCTGG TGACTCTGAC CAGCAACTAT GACATCCCAG AGGAATGGGC TCAATTCTAT 
ACGGCAGAAG TGGTGCTGGC ACTCGACGCC ATCCATTCTC TGGGCTTCAT CCACAGAGAC 
ATCAAACCAG ACAACATGCT GCTGGACCGC AATGGACACC TCAAACTCGC AGACTTTGGC 
ACATGCATGA AGATGGACTC GACTGGTATG GTACGCTGTG ACACTGCTGT TGGGACACCT 
GATTACATTT CTCCAGAGGT TTTAATGTCT CAAGGAGGGA CTGGTTATTA CGGTCGCGAG 
TGTGACTGGT GGTCTGTTGG TGTTTTTATT TATGAGCTTC TAGTGGGCGA CACTCCATTT 
TATTCCGAGT CTCTGGTGGG CACATATGGA AAGATCATGG ATCACAAGAA CAGCTTAACT 
TTCCCAGATG ATATTGAAAT GTCAAAGAAT GCCAAAGACC TCATCTGTGC CTTTCTGAGT 
AGCAGGGAGG TGAGGCTTGG CCGAACTGGA GTGGATGAAA TAAAATGTCA CCCCTTCTTT 
AAGAATGATC AGTGGACCTT CGACACCATA CGAGACACCA TGGCTCCAGT GGTGCCAGAG 
CTGAGTAGCG ATATTGACAC CAGTAACTTT GATGATGACA TTAAGGATGA TCCGATAGGA 
ACAGAGACGT TCCCTCCGCC CCGTGCCTTC GCAGGGAACC AGCTACCATT TGTGGGTTTC 
ACCTATTTCA GGGAGGACCA ATTGTTAACA CAACAAAATA AGTGTTCAGA GGAAGATATC 
ATTGAAGATC ATGGCTTAAA CCACACAGAG TCTAACGACC TGGAGAAGAG GCTGAAAAAG 
TTGGAGGAAA AATTCAAACA TGAGATGCAA GCAAAGGAAG AACTGGAAAA TAAATGCCGA 
ATAGCAAATC AGCGGCTGGA GAAATTGTCC AAAGATCTTG AAGAAGAAGT GAATGCGAGA 
CAGGAGGCGG AAGACAACCT GAGATCCCTG GAGAAAGAGA AAGTTTTACT GAAACACCAG 
CGTACACAGA GCGTAAGAAA AGCTGGTCTA GAGACCGACA GAAAACGGCT GCTGGAGAAT 
GAAGTAAGCA GCCTGAAAGA GCAGCTTGCC GAACTGAAGA AAAAGAACCA GATCTCTCAT 
CTGTCTGCTG AGAAGAACAT TCACCTGGAA AGACAGCTGG AGGAGGTGAG TGCCAAGCTT 
CAGGCCGAGC TGGAGGAGTC GGAGAGGCTG AAGAAGGCCC AGATCGAGGC CTTTAGACAG 
TCCCAGCAGC TGGAGCTTTC CCTCAGGGAG CTGCAGGAGC GTCTGGCCCA GCTGGAGAAC 
AGCAGGCTGG TTCTGGAGCA GGACAAACTC AGCCTGCAGA CCTCGCTAGA GCTGGAGAAA 
CGAGAGAGAA ACGCCGGCTC AGAGACCATC ACTGACCTGC AGGGTCGTAT CTATGGACTG 
GAGGGTGAGT TGAAGCATAT AAAGAGTTCT CTATCAAAAG CTGAAGTTGA GAAAAGACAA 
TTACAAGAGG ATCTTACAAC CCTGGAAAAG GAGAAGAATA AGCAGGAGAT CGACCTCTCC 
TTCAAACTCA AAGCAGTTCA GCAGAGTCTG GAGCAGGAGG AGGCTGAACA TAAAACCACT 
AAAGCCAGAT TGGCCGACAA CAACAAAATC AACCAATCCA TAGAGGCAAA GTCTGAGACC 
CTGAAAGACA TGGAACATAA GCTGTTGGAA GAACGATCTG CGAAGCAACA ACTGGAAAAC 
AGACTGATGC AGCTGGAGAA GGAAAACTCT GTATTAGACT GTGATTACAA ACAAGCCAAA 
CATGAGCTTC AGGAGCTCCG CTCGCTCAAA GAAAATCTCA CAGAGCAGGT GGAGGTGCTG 
AATGTGCGGG TGCAGCAGGA GACACAGAGG AAAACTCTCT GTCAAGGTGA TCTCAAAGTT 
CAGAGGCAGG AGATCAACAG CCTGCGGTCA TCAGAACAAC AGCTCAAACA AGAACTCAAC 
CATCTGCTGG AGCTCAAACT CACACTGGAG AAACAAAACC AGGAGCTGTC CAAGGAACGA 
GAAGAATCTG AAAAACAGCT GAAGGAGATG AAGGACCAGC TGGAGGCTGA GCAGTACTTC 
ACGAAACTGT ACAAGACTCA GATTCGAGAG CTGAAAGAGG AAAGCGATGA AAAGGTTAAA 
CTCTACAAAG ATGCTCAGCA GAGAATAGAG GACCTGCAGG AAGAGAGGGA CTCGTTAGCG 
TCTCAGTTGG AGGTGAGCCT GACGAAGGCT GACTCTGAAC AGTTGGCTCG CATCACCGTT 
GAAGAGCAAT ACTCGGACCT GGAGAAAGAG AAGATCATGA AGGAACTGGA GATAAAGGAC 
ATGATCGCCA GACACCGGCA GGATCTTGCA GAAAAGGACG GTACCATCAA CTCGCTGGAG 
GAGTCAAACC GTACGCTGAC TGTGGATGTT GCCAACCTGG CCAGTGAGAA AGAGGAGCTC 
AACAATAAAC TGAAGCACAT ACAGCAAAAG CTGGAGAAGA TCAAAGAAGA AGAGAAACAG 
ATGAAATCTC TCACAGTGTC GTATGAGAAG CAGATACAGG TGGAGAAAAC ACTCAAGATT 
CAGGCTATAA ACAAGCTGGC GGAGGTCATG AAGAAGACGG ACGGCAGGCC ACACCAAATC 
AACAACACAG ACATCCGCAG GAAGGAGAAG GAGATCAGAC AACTCCAGCT GCAGCTCCGC 
ACGGAGAAAG AAAAGCTTAA CCACTCCATC ATCAAATACC AGAGAGAAAT CAACGACATG 
CAGGCTATGA TAGCAGAGGA GTCTCAGGTG CGTCTGGAGA TGCAGATGTC TCTGGACAGT 
AAAGACAGTG ATATTGAGCG TCTGCGCAGT CAACTCACGT CTCTGAGCAT TCACTCTCTG 
GACACCACCA GCATCAGCAG CATCGGCAAT GACCTGGATT CTGACGAGGC ATATCCAGCC 
GCAGAGTCCG GTCTTGAAGG ATGGCTCTCA ATGCCTGCCA AAAACACCAA ACGGTTTGGA 
TGGGATAAGA AGTATGTGGT TGTGAGCAGC AAGAAAATCC TGTTTTATGA CTCTGAACAA 
GACCGAGAGC AGTCCAGTCC ATTCATGATC CTGGACATTG ACAAACTCTT CCACGTGAGA 
CCCGTCACTC AGACAGATGT TTACCGCGCA GATCCCAGAG AAATCCCTCG CATCTTCCAG 
ATACTATATG CGAATGAAGG AGAGAGCAAG AAAGAGGAGC TGCCCGTGGA GTCGTTATCT 
GTGACTGAGA GGTCACTGTG TATCCCACAC AAGGGTCACG AGTTTGTTCT GACGCTTTAT 
CACTTCCCCT CGAGCTGCGA GGTCTGTCCA CGACCCTTAT GGCACGTCTT CAGACCTCCG 
CCGGCTCTCG AGTGCAGACG CTGTAGAGTC AAGTGCCATA AAGACCACAT CGATCGCAAA 
GAAGAGGTCC TCGCTCCATG TAGAGTGAAT TACGACATGT CCACAGCGAA AGAGATGCTG 
CTGCTGGCAA ACTCCACAGA GGAGCAGAAG AAGTGGGTCA GTCGGCTGCT CAAGAGGGTT 
CCGCGTAAAC CTTCCATCGC CCACTCTTCC AGCATAGCCA TATCCGCTGC GCCCTCAGAG 
GCAACACCCA CTACCCTTTC CCCTCAGCCG TCGCCTCGCC TCGCTCAGTC TTCACCCAGA 
CTTTCACACC GAGCAGCCGT CAAAGTGCAC TCTACACGAC AGCAGCCCTC CAGCAAGAAC 
AGATTGCTTG AAATGGGCCT GAAGGACTGG GGTTGGGCTC TTGAGGTTGA ATTGGATGAT 
GACGATGTTT TAGACTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017207923.1
CDS	583..4863
Translation	MSTGAGKRIESRVRKLETLIKNQKSPLNLESLLDSVTALALDLNHPALRKNKNIDAFLSRYEKAVCNLKELQVKLDDFDRVKLIGRGAFGAVQLVRHKVSQQVYAMKQLSKFEMVKRSDSAFFWEERDIMAFSQSPWIVQLCCAFQDEKYLYLVMEFMPGGDLVTLTSNYDIPEEWAQFYTAEVVLALDAIHSLGFIHRDIKPDNMLLDRNGHLKLADFGTCMKMDSTGMVRCDTAVGTPDYISPEVLMSQGGTGYYGRECDWWSVGVFIYELLVGDTPFYSESLVGTYGKIMDHKNSLTFPDDIEMSKNAKDLICAFLSSREVRLGRTGVDEIKCHPFFKNDQWTFDTIRDTMAPVVPELSSDIDTSNFDDDIKDDPIGTETFPPPRAFAGNQLPFVGFTYFREDQLLTQQNKCSEEDIIEDHGLNHTESNDLEKRLKKLEEKFKHEMQAKEELENKCRIANQRLEKLSKDLEEEVNARQEAEDNLRSLEKEKVLLKHQRTQSVRKAGLETDRKRLLENEVSSLKEQLAELKKKNQISHLSAEKNIHLERQLEEVSAKLQAELEESERLKKAQIEAFRQSQQLELSLRELQERLAQLENSRLVLEQDKLSLQTSLELEKRERNAGSETITDLQGRIYGLEGELKHIKSSLSKAEVEKRQLQEDLTTLEKEKNKQEIDLSFKLKAVQQSLEQEEAEHKTTKARLADNNKINQSIEAKSETLKDMEHKLLEERSAKQQLENRLMQLEKENSVLDCDYKQAKHELQELRSLKENLTEQVEVLNVRVQQETQRKTLCQGDLKVQRQEINSLRSSEQQLKQELNHLLELKLTLEKQNQELSKEREESEKQLKEMKDQLEAEQYFTKLYKTQIRELKEESDEKVKLYKDAQQRIEDLQEERDSLASQLEVSLTKADSEQLARITVEEQYSDLEKEKIMKELEIKDMIARHRQDLAEKDGTINSLEESNRTLTVDVANLASEKEELNNKLKHIQQKLEKIKEEEKQMKSLTVSYEKQIQVEKTLKIQAINKLAEVMKKTDGRPHQINNTDIRRKEKEIRQLQLQLRTEKEKLNHSIIKYQREINDMQAMIAEESQVRLEMQMSLDSKDSDIERLRSQLTSLSIHSLDTTSISSIGNDLDSDEAYPAAESGLEGWLSMPAKNTKRFGWDKKYVVVSSKKILFYDSEQDREQSSPFMILDIDKLFHVRPVTQTDVYRADPREIPRIFQILYANEGESKKEELPVESLSVTERSLCIPHKGHEFVLTLYHFPSSCEVCPRPLWHVFRPPPALECRRCRVKCHKDHIDRKEEVLAPCRVNYDMSTAKEMLLLANSTEEQKKWVSRLLKRVPRKPSIAHSSSIAISAAPSEATPTTLSPQPSPRLAQSSPRLSHRAAVKVHSTRQQPSSKNRLLEMGLKDWGWALEVELDDDDVLDF

Target ORF information:

RefSeq Version	XM_017352434.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017352434.1

ORF Insert Sequence:

ATGTCGACCG GAGCGGGGAA GAGAATAGAG AGCCGGGTGA GAAAACTAGA GACGCTGATC 
AAGAACCAGA AATCACCGTT GAACTTGGAG AGTTTGCTGG ATTCAGTAAC TGCCCTTGCC 
CTTGATCTGA ACCATCCAGC ACTGAGAAAG AACAAAAACA TAGATGCCTT CTTAAGCAGA 
TATGAAAAAG CCGTGTGCAA CCTCAAGGAA CTACAGGTGA AGCTGGATGA TTTTGATCGA 
GTCAAGCTGA TCGGTCGTGG AGCGTTTGGC GCAGTGCAGC TGGTACGGCA CAAAGTATCT 
CAACAAGTTT ATGCCATGAA GCAGCTCAGC AAGTTTGAGA TGGTCAAGCG CTCTGACTCC 
GCCTTTTTCT GGGAGGAGCG TGACATCATG GCTTTCTCCC AGAGCCCTTG GATAGTTCAG 
CTGTGCTGTG CATTCCAAGA TGAAAAGTAC CTTTATTTGG TGATGGAGTT CATGCCAGGT 
GGAGATCTGG TGACTCTGAC CAGCAACTAT GACATCCCAG AGGAATGGGC TCAATTCTAT 
ACGGCAGAAG TGGTGCTGGC ACTCGACGCC ATCCATTCTC TGGGCTTCAT CCACAGAGAC 
ATCAAACCAG ACAACATGCT GCTGGACCGC AATGGACACC TCAAACTCGC AGACTTTGGC 
ACATGCATGA AGATGGACTC GACTGGTATG GTACGCTGTG ACACTGCTGT TGGGACACCT 
GATTACATTT CTCCAGAGGT TTTAATGTCT CAAGGAGGGA CTGGTTATTA CGGTCGCGAG 
TGTGACTGGT GGTCTGTTGG TGTTTTTATT TATGAGCTTC TAGTGGGCGA CACTCCATTT 
TATTCCGAGT CTCTGGTGGG CACATATGGA AAGATCATGG ATCACAAGAA CAGCTTAACT 
TTCCCAGATG ATATTGAAAT GTCAAAGAAT GCCAAAGACC TCATCTGTGC CTTTCTGAGT 
AGCAGGGAGG TGAGGCTTGG CCGAACTGGA GTGGATGAAA TAAAATGTCA CCCCTTCTTT 
AAGAATGATC AGTGGACCTT CGACACCATA CGAGACACCA TGGCTCCAGT GGTGCCAGAG 
CTGAGTAGCG ATATTGACAC CAGTAACTTT GATGATGACA TTAAGGATGA TCCGATAGGA 
ACAGAGACGT TCCCTCCGCC CCGTGCCTTC GCAGGGAACC AGCTACCATT TGTGGGTTTC 
ACCTATTTCA GGGAGGACCA ATTGTTAACA CAACAAAATA AGTGTTCAGA GGAAGATATC 
ATTGAAGATC ATGGCTTAAA CCACACAGAG TCTAACGACC TGGAGAAGAG GCTGAAAAAG 
TTGGAGGAAA AATTCAAACA TGAGATGCAA GCAAAGGAAG AACTGGAAAA TAAATGCCGA 
ATAGCAAATC AGCGGCTGGA GAAATTGTCC AAAGATCTTG AAGAAGAAGT GAATGCGAGA 
CAGGAGGCGG AAGACAACCT GAGATCCCTG GAGAAAGAGA AAGTTTTACT GAAACACCAG 
CGTACACAGA GCGTAAGAAA AGCTGGTCTA GAGACCGACA GAAAACGGCT GCTGGAGAAT 
GAAGTAAGCA GCCTGAAAGA GCAGCTTGCC GAACTGAAGA AAAAGAACCA GATCTCTCAT 
CTGTCTGCTG AGAAGAACAT TCACCTGGAA AGACAGCTGG AGGAGGTGAG TGCCAAGCTT 
CAGGCCGAGC TGGAGGAGTC GGAGAGGCTG AAGAAGGCCC AGATCGAGGC CTTTAGACAG 
TCCCAGCAGC TGGAGCTTTC CCTCAGGGAG CTGCAGGAGC GTCTGGCCCA GCTGGAGAAC 
AGCAGGCTGG TTCTGGAGCA GGACAAACTC AGCCTGCAGA CCTCGCTAGA GCTGGAGAAA 
CGAGAGAGAA ACGCCGGCTC AGAGACCATC ACTGACCTGC AGGGTCGTAT CTATGGACTG 
GAGGGTGAGT TGAAGCATAT AAAGAGTTCT CTATCAAAAG CTGAAGTTGA GAAAAGACAA 
TTACAAGAGG ATCTTACAAC CCTGGAAAAG GAGAAGAATA AGCAGGAGAT CGACCTCTCC 
TTCAAACTCA AAGCAGTTCA GCAGAGTCTG GAGCAGGAGG AGGCTGAACA TAAAACCACT 
AAAGCCAGAT TGGCCGACAA CAACAAAATC AACCAATCCA TAGAGGCAAA GTCTGAGACC 
CTGAAAGACA TGGAACATAA GCTGTTGGAA GAACGATCTG CGAAGCAACA ACTGGAAAAC 
AGACTGATGC AGCTGGAGAA GGAAAACTCT GTATTAGACT GTGATTACAA ACAAGCCAAA 
CATGAGCTTC AGGAGCTCCG CTCGCTCAAA GAAAATCTCA CAGAGCAGGT GGAGGTGCTG 
AATGTGCGGG TGCAGCAGGA GACACAGAGG AAAACTCTCT GTCAAGGTGA TCTCAAAGTT 
CAGAGGCAGG AGATCAACAG CCTGCGGTCA TCAGAACAAC AGCTCAAACA AGAACTCAAC 
CATCTGCTGG AGCTCAAACT CACACTGGAG AAACAAAACC AGGAGCTGTC CAAGGAACGA 
GAAGAATCTG AAAAACAGCT GAAGGAGATG AAGGACCAGC TGGAGGCTGA GCAGTACTTC 
ACGAAACTGT ACAAGACTCA GATTCGAGAG CTGAAAGAGG AAAGCGATGA AAAGGTTAAA 
CTCTACAAAG ATGCTCAGCA GAGAATAGAG GACCTGCAGG AAGAGAGGGA CTCGTTAGCG 
TCTCAGTTGG AGGTGAGCCT GACGAAGGCT GACTCTGAAC AGTTGGCTCG CATCACCGTT 
GAAGAGCAAT ACTCGGACCT GGAGAAAGAG AAGATCATGA AGGAACTGGA GATAAAGGAC 
ATGATCGCCA GACACCGGCA GGATCTTGCA GAAAAGGACG GTACCATCAA CTCGCTGGAG 
GAGTCAAACC GTACGCTGAC TGTGGATGTT GCCAACCTGG CCAGTGAGAA AGAGGAGCTC 
AACAATAAAC TGAAGCACAT ACAGCAAAAG CTGGAGAAGA TCAAAGAAGA AGAGAAACAG 
ATGAAATCTC TCACAGTGTC GTATGAGAAG CAGATACAGG TGGAGAAAAC ACTCAAGATT 
CAGGCTATAA ACAAGCTGGC GGAGGTCATG AAGAAGACGG ACGGCAGGCC ACACCAAATC 
AACAACACAG ACATCCGCAG GAAGGAGAAG GAGATCAGAC AACTCCAGCT GCAGCTCCGC 
ACGGAGAAAG AAAAGCTTAA CCACTCCATC ATCAAATACC AGAGAGAAAT CAACGACATG 
CAGGCTATGA TAGCAGAGGA GTCTCAGGTG CGTCTGGAGA TGCAGATGTC TCTGGACAGT 
AAAGACAGTG ATATTGAGCG TCTGCGCAGT CAACTCACGT CTCTGAGCAT TCACTCTCTG 
GACACCACCA GCATCAGCAG CATCGGCAAT GACCTGGATT CTGACGAGGC ATATCCAGCC 
GCAGAGTCCG GTCTTGAAGG ATGGCTCTCA ATGCCTGCCA AAAACACCAA ACGGTTTGGA 
TGGGATAAGA AGTATGTGGT TGTGAGCAGC AAGAAAATCC TGTTTTATGA CTCTGAACAA 
GACCGAGAGC AGTCCAGTCC ATTCATGATC CTGGACATTG ACAAACTCTT CCACGTGAGA 
CCCGTCACTC AGACAGATGT TTACCGCGCA GATCCCAGAG AAATCCCTCG CATCTTCCAG 
ATACTATATG CGAATGAAGG AGAGAGCAAG AAAGAGGAGC TGCCCGTGGA GTCGTTATCT 
GTGACTGAGA GGTCACTGTG TATCCCACAC AAGGGTCACG AGTTTGTTCT GACGCTTTAT 
CACTTCCCCT CGAGCTGCGA GGTCTGTCCA CGACCCTTAT GGCACGTCTT CAGACCTCCG 
CCGGCTCTCG AGTGCAGACG CTGTAGAGTC AAGTGCCATA AAGACCACAT CGATCGCAAA 
GAAGAGGTCC TCGCTCCATG TAGAGTGAAT TACGACATGT CCACAGCGAA AGAGATGCTG 
CTGCTGGCAA ACTCCACAGA GGAGCAGAAG AAGTGGGTCA GTCGGCTGCT CAAGAGGGTT 
CCGCGTAAAC CTTCCATCGC CCACTCTTCC AGCATAGCCA TATCCGCTGC GCCCTCAGAG 
GCAACACCCA CTACCCTTTC CCCTCAGCCG TCGCCTCGCC TCGCTCAGTC TTCACCCAGA 
CTTTCACACC GAGCAGCCGT CAAAGTGCAC TCTACACGAC AGCAGCCCTC CAGCAAGAAC 
AGATTGCTTG AAATGGGCCT GAAGGACTGG GGTTGGGCTC TTGAGGTTGA ATTGGATGAT 
GACGATGTTT TAGACTTCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rock2b ( XM_021468360.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324035.1 Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X7, mRNA.

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CloneID	ODa150580
Clone ID Related Accession (Same CDS sequence)	XM_021468360.1
Accession Version	XM_021468360.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4242bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho-associated, coiled-coil containing protein kinase 2b isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGACCG GAGCGGGGAA GAGAATAGAG AGCCGGGTGA GAAAACTAGA GACGCTGATC 
AAGAACCAGA AATCACCGTT GAACTTGGAG AGTTTGCTGG ATTCAGTAAC TGCCCTTGCC 
CTTGATCTGA ACCATCCAGC ACTGAGAAAG AACAAAAACA TAGATGCCTT CTTAAGCAGA 
TATGAAAAAG CCGTGTGCAA CCTCAAGGAA CTACAGGTGA AGCTGGATGA TTTTGATCGA 
GTCAAGCTGA TCGGTCGTGG AGCGTTTGGC GCAGTGCAGC TGGTACGGCA CAAAGTATCT 
CAACAAGTTT ATGCCATGAA GCAGCTCAGC AAGTTTGAGA TGGTCAAGCG CTCTGACTCC 
GCCTTTTTCT GGGAGGAGCG TGACATCATG GCTTTCTCCC AGAGCCCTTG GATAGTTCAG 
CTGTGCTGTG CATTCCAAGA TGAAAAGTAC CTTTATTTGG TGATGGAGTT CATGCCAGGT 
GGAGATCTGG TGACTCTGAC CAGCAACTAT GACATCCCAG AGGAATGGGC TCAATTCTAT 
ACGGCAGAAG TGGTGCTGGC ACTCGACGCC ATCCATTCTC TGGGCTTCAT CCACAGAGAC 
ATCAAACCAG ACAACATGCT GCTGGACCGC AATGGACACC TCAAACTCGC AGACTTTGGC 
ACATGCATGA AGATGGACTC GACTGGTATG GTACGCTGTG ACACTGCTGT TGGGACACCT 
GATTACATTT CTCCAGAGGT TTTAATGTCT CAAGGAGGGA CTGGTTATTA CGGTCGCGAG 
TGTGACTGGT GGTCTGTTGG TGTTTTTATT TATGAGCTTC TAGTGGGCGA CACTCCATTT 
TATTCCGAGT CTCTGGTGGG CACATATGGA AAGATCATGG ATCACAAGAA CAGCTTAACT 
TTCCCAGATG ATATTGAAAT GTCAAAGAAT GCCAAAGACC TCATCTGTGC CTTTCTGAGT 
AGCAGGGAGG TGAGGCTTGG CCGAACTGGA GTGGATGAAA TAAAATGTCA CCCCTTCTTT 
AAGAATGATC AGTGGACCTT CGACACCATA CGAGACACCA TGGCTCCAGT GGTGCCAGAG 
CTGAGTAGCG ATATTGACAC CAGTAACTTT GATGATGACA TTAAGGATGA TCCGATAGGA 
ACAGAGACGT TCCCTCCGCC CCGTGCCTTC GCAGGGAACC AGCTACCATT TGTGGGTTTC 
ACCTATTTCA GGGAGGACCA ATTGTTAACA CAACAAAATA AGTGTTCAGA GGAAGATATC 
ATTGAAGATC ATGGCTTAAA CCACACAGAG TCTAACGACC TGGAGAAGAG GCTGAAAAAG 
TTGGAGGAAA AATTCAAACA TGAGATGCAA GCAAAGGAAG AACTGGAAAA TAAATGCCGA 
ATAGCAAATC AGCGGCTGGA GAAATTGTCC AAAGATCTTG AAGAAGAAGT GAATGCGAGA 
CAGGAGGCGG AAGACAACCT GAGATCCCTG GAGAAAGAGA AAGTTTTACT GAAACACCAG 
CGTACACAGA GCGTAAGAAA AGCTGGTCTA GAGACCGACA GAAAACGGCT GCTGGAGAAT 
GAAGTAAGCA GCCTGAAAGA GCAGCTTGCC GAACTGAAGA AAAAGAACCA GATCTCTCAT 
CTGTCTGCTG AGAAGAACAT TCACCTGGAA AGACAGCTGG AGGAGGTGAG TGCCAAGCTT 
CAGGCCGAGC TGGAGGAGTC GGAGAGGCTG AAGAAGGCCC AGATCGAGGC CTTTAGACAG 
TCCCAGCAGC TGGAGCTTTC CCTCAGGGAG CTGCAGGAGC GTCTGGCCCA GCTGGAGAAC 
AGCAGGCTGG TTCTGGAGCA GGACAAACTC AGCCTGCAGA CCTCGCTAGA GCTGGAGAAA 
CGAGAGAGAA ACGCCGGCTC AGAGACCATC ACTGACCTGC AGGGTCGTAT CTATGGACTG 
GAGGGTGAGT TGAAGCATAT AAAGAGTTCT CTATCAAAAG CTGAAGTTGA GAAAAGACAA 
TTACAAGAGG ATCTTACAAC CCTGGAAAAG GAGAAGAATA AGCAGGAGAT CGACCTCTCC 
TTCAAACTCA AAGCAGTTCA GCAGAGTCTG GAGCAGGAGG AGGCTGAACA TAAAACCACT 
AAAGCCAGAT TGGCCGACAA CAACAAAATC AACCAATCCA TAGAGGCAAA GTCTGAGACC 
CTGAAAGACA TGGAACATAA GCTGTTGGAA GAACGATCTG CGAAGCAACA ACTGGAAAAC 
AGACTGATGC AGCTGGAGAA GGAAAACTCT GTATTAGACT GTGATTACAA ACAAGCCAAA 
CATGAGCTTC AGGAGCTCCG CTCGCTCAAA GAAAATCTCA CAGAGCAGGT GGAGGTGCTG 
AATGTGCGGG TGCAGCAGGA GACACAGAGG AAAACTCTCT GTCAAGGTGA TCTCAAAGTT 
CAGAGGCAGG AGATCAACAG CCTGCGGTCA TCAGAACAAC AGCTCAAACA AGAACTCAAC 
CATCTGCTGG AGCTCAAACT CACACTGGAG AAACAAAACC AGGAGCTGTC CAAGGAACGA 
GAAGAATCTG AAAAACAGCT GAAGGAGATG AAGGACCAGC TGGAGGCTGA GCAGTACTTC 
ACGAAACTGT ACAAGACTCA GATTCGAGAG CTGAAAGAGG AAAGCGATGA AAAGGTTAAA 
CTCTACAAAG ATGCTCAGCA GAGAATAGAG GACCTGCAGG AAGAGAGGGA CTCGTTAGCG 
TCTCAGTTGG AGGTGAGCCT GACGAAGGCT GACTCTGAAC AGTTGGCTCG CATCACCGTT 
GAAGAGCAAT ACTCGGACCT GGAGAAAGAG AAGATCATGA AGGAACTGGA GATAAAGGAC 
ATGATCGCCA GACACCGGCA GGATCTTGCA GAAAAGGACG GTACCATCAA CTCGCTGGAG 
GAGTCAAACC GTACGCTGAC TGTGGATGTT GCCAACCTGG CCAGTGAGAA AGAGGAGCTC 
AACAATAAAC TGAAGCACAT ACAGCAAAAG CTGGAGAAGA TCAAAGAAGA AGAGAAACAG 
ATGAAATCTC TCACAGTGTC GTATGAGAAG CAGATACAGG TGGAGAAAAC ACTCAAGATT 
CAGGCTATAA ACAAGCTGGC GGAGGTCATG AAGAAGACGG ACGGCAGGCC ACACCAAATC 
AACAACACAG ACATCCGCAG GAAGGAGAAG GAGATCAGAC AACTCCAGCT GCAGCTCCGC 
ACGGAGAAAG AAAAGCTTAA CCACTCCATC ATCAAATACC AGAGAGAAAT CAACGACATG 
CAGGCTATGA TAGCAGAGGA GTCTCAGGTG CGTCTGGAGA TGCAGATGTC TCTGGACAGT 
AAAGACAGTG ATATTGAGCG TCTGCGCAGT CAACTCACGT CTCTGAGCAT TCACTCTCTG 
GACACCACCA GCATCAGCAG CATCGGCAAT GACCTGGATT CTGACGAGGC ATATCCAGTG 
CGAATCACAC ACTCACACAC CTCGGAGTCT ATGTCCTTCA CATACCAGCG AAGCTCCAAG 
TCGGTGTGTA TTGATACCCG GCCCTGTCGA ATCGCTCCTC GCTTCTCCTC TGACTCTGAT 
GAAGAGGAGG ATGAGGAAGA TGAGTGGAGA CAAACACCTG CCCTTACATA TGAGACGCCT 
CCTGACCCTG AGGCTGCAGC CGCAGAGTCC GGTCTTGAAG GATGGCTCTC AATGCCTGCC 
AAAAACACCA AACGGTTTGG ATGGGATAAG AAGCTGTGCT GTGCATTCCA AGATGAAAAG 
TACCTTTATT TGGTGATGGA GTTCATGCCA GGTGGAGATC TGGTGACTCT GACCAGCAAC 
TATGACATCC CAGAGGAATG GGCTCAATTC TATACGGCAG AAGTGGTGCT GGCACTCGAC 
GCCATCCATT CTCTGGGCTT CATCCACAGA GACATCAAAC CAGACAACAT GCTGCTGGAC 
CGCAATGGAC ACCTCAAACT CGCAGACTTT GGCACATGCA TGAAGATGGA CTCGACTGGT 
TTGGTAAGCT GTGACGCTGC TGTTGGGACA CCTGATTACA TTTCTCCAGA GGTTTTAATG 
TCTCAAGGAG GGACTGGTTA TTACGGTCGC GAGTGTGACT GGTGGTCTGT TGGTGTTTTT 
ATTTATGAGC TTCTAGTGGG CGACACTCCA TTTTATTCCG AGTCTCTGGT GGGCACATAT 
GGAAAGATCA TGGATCACAA GAACAGCTTA ACTTTCCCAG ATGATATTGA AATGTCAAAG 
GATGCCAAAG ACCTCATCTG TGCCTTTCTG AGTAGCAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324035.1
CDS	478..4719
Translation	MSTGAGKRIESRVRKLETLIKNQKSPLNLESLLDSVTALALDLNHPALRKNKNIDAFLSRYEKAVCNLKELQVKLDDFDRVKLIGRGAFGAVQLVRHKVSQQVYAMKQLSKFEMVKRSDSAFFWEERDIMAFSQSPWIVQLCCAFQDEKYLYLVMEFMPGGDLVTLTSNYDIPEEWAQFYTAEVVLALDAIHSLGFIHRDIKPDNMLLDRNGHLKLADFGTCMKMDSTGMVRCDTAVGTPDYISPEVLMSQGGTGYYGRECDWWSVGVFIYELLVGDTPFYSESLVGTYGKIMDHKNSLTFPDDIEMSKNAKDLICAFLSSREVRLGRTGVDEIKCHPFFKNDQWTFDTIRDTMAPVVPELSSDIDTSNFDDDIKDDPIGTETFPPPRAFAGNQLPFVGFTYFREDQLLTQQNKCSEEDIIEDHGLNHTESNDLEKRLKKLEEKFKHEMQAKEELENKCRIANQRLEKLSKDLEEEVNARQEAEDNLRSLEKEKVLLKHQRTQSVRKAGLETDRKRLLENEVSSLKEQLAELKKKNQISHLSAEKNIHLERQLEEVSAKLQAELEESERLKKAQIEAFRQSQQLELSLRELQERLAQLENSRLVLEQDKLSLQTSLELEKRERNAGSETITDLQGRIYGLEGELKHIKSSLSKAEVEKRQLQEDLTTLEKEKNKQEIDLSFKLKAVQQSLEQEEAEHKTTKARLADNNKINQSIEAKSETLKDMEHKLLEERSAKQQLENRLMQLEKENSVLDCDYKQAKHELQELRSLKENLTEQVEVLNVRVQQETQRKTLCQGDLKVQRQEINSLRSSEQQLKQELNHLLELKLTLEKQNQELSKEREESEKQLKEMKDQLEAEQYFTKLYKTQIRELKEESDEKVKLYKDAQQRIEDLQEERDSLASQLEVSLTKADSEQLARITVEEQYSDLEKEKIMKELEIKDMIARHRQDLAEKDGTINSLEESNRTLTVDVANLASEKEELNNKLKHIQQKLEKIKEEEKQMKSLTVSYEKQIQVEKTLKIQAINKLAEVMKKTDGRPHQINNTDIRRKEKEIRQLQLQLRTEKEKLNHSIIKYQREINDMQAMIAEESQVRLEMQMSLDSKDSDIERLRSQLTSLSIHSLDTTSISSIGNDLDSDEAYPVRITHSHTSESMSFTYQRSSKSVCIDTRPCRIAPRFSSDSDEEEDEEDEWRQTPALTYETPPDPEAAAAESGLEGWLSMPAKNTKRFGWDKKLCCAFQDEKYLYLVMEFMPGGDLVTLTSNYDIPEEWAQFYTAEVVLALDAIHSLGFIHRDIKPDNMLLDRNGHLKLADFGTCMKMDSTGLVSCDAAVGTPDYISPEVLMSQGGTGYYGRECDWWSVGVFIYELLVGDTPFYSESLVGTYGKIMDHKNSLTFPDDIEMSKDAKDLICAFLSSR

Target ORF information:

RefSeq Version	XM_021468360.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468360.1

ORF Insert Sequence:

ATGTCGACCG GAGCGGGGAA GAGAATAGAG AGCCGGGTGA GAAAACTAGA GACGCTGATC 
AAGAACCAGA AATCACCGTT GAACTTGGAG AGTTTGCTGG ATTCAGTAAC TGCCCTTGCC 
CTTGATCTGA ACCATCCAGC ACTGAGAAAG AACAAAAACA TAGATGCCTT CTTAAGCAGA 
TATGAAAAAG CCGTGTGCAA CCTCAAGGAA CTACAGGTGA AGCTGGATGA TTTTGATCGA 
GTCAAGCTGA TCGGTCGTGG AGCGTTTGGC GCAGTGCAGC TGGTACGGCA CAAAGTATCT 
CAACAAGTTT ATGCCATGAA GCAGCTCAGC AAGTTTGAGA TGGTCAAGCG CTCTGACTCC 
GCCTTTTTCT GGGAGGAGCG TGACATCATG GCTTTCTCCC AGAGCCCTTG GATAGTTCAG 
CTGTGCTGTG CATTCCAAGA TGAAAAGTAC CTTTATTTGG TGATGGAGTT CATGCCAGGT 
GGAGATCTGG TGACTCTGAC CAGCAACTAT GACATCCCAG AGGAATGGGC TCAATTCTAT 
ACGGCAGAAG TGGTGCTGGC ACTCGACGCC ATCCATTCTC TGGGCTTCAT CCACAGAGAC 
ATCAAACCAG ACAACATGCT GCTGGACCGC AATGGACACC TCAAACTCGC AGACTTTGGC 
ACATGCATGA AGATGGACTC GACTGGTATG GTACGCTGTG ACACTGCTGT TGGGACACCT 
GATTACATTT CTCCAGAGGT TTTAATGTCT CAAGGAGGGA CTGGTTATTA CGGTCGCGAG 
TGTGACTGGT GGTCTGTTGG TGTTTTTATT TATGAGCTTC TAGTGGGCGA CACTCCATTT 
TATTCCGAGT CTCTGGTGGG CACATATGGA AAGATCATGG ATCACAAGAA CAGCTTAACT 
TTCCCAGATG ATATTGAAAT GTCAAAGAAT GCCAAAGACC TCATCTGTGC CTTTCTGAGT 
AGCAGGGAGG TGAGGCTTGG CCGAACTGGA GTGGATGAAA TAAAATGTCA CCCCTTCTTT 
AAGAATGATC AGTGGACCTT CGACACCATA CGAGACACCA TGGCTCCAGT GGTGCCAGAG 
CTGAGTAGCG ATATTGACAC CAGTAACTTT GATGATGACA TTAAGGATGA TCCGATAGGA 
ACAGAGACGT TCCCTCCGCC CCGTGCCTTC GCAGGGAACC AGCTACCATT TGTGGGTTTC 
ACCTATTTCA GGGAGGACCA ATTGTTAACA CAACAAAATA AGTGTTCAGA GGAAGATATC 
ATTGAAGATC ATGGCTTAAA CCACACAGAG TCTAACGACC TGGAGAAGAG GCTGAAAAAG 
TTGGAGGAAA AATTCAAACA TGAGATGCAA GCAAAGGAAG AACTGGAAAA TAAATGCCGA 
ATAGCAAATC AGCGGCTGGA GAAATTGTCC AAAGATCTTG AAGAAGAAGT GAATGCGAGA 
CAGGAGGCGG AAGACAACCT GAGATCCCTG GAGAAAGAGA AAGTTTTACT GAAACACCAG 
CGTACACAGA GCGTAAGAAA AGCTGGTCTA GAGACCGACA GAAAACGGCT GCTGGAGAAT 
GAAGTAAGCA GCCTGAAAGA GCAGCTTGCC GAACTGAAGA AAAAGAACCA GATCTCTCAT 
CTGTCTGCTG AGAAGAACAT TCACCTGGAA AGACAGCTGG AGGAGGTGAG TGCCAAGCTT 
CAGGCCGAGC TGGAGGAGTC GGAGAGGCTG AAGAAGGCCC AGATCGAGGC CTTTAGACAG 
TCCCAGCAGC TGGAGCTTTC CCTCAGGGAG CTGCAGGAGC GTCTGGCCCA GCTGGAGAAC 
AGCAGGCTGG TTCTGGAGCA GGACAAACTC AGCCTGCAGA CCTCGCTAGA GCTGGAGAAA 
CGAGAGAGAA ACGCCGGCTC AGAGACCATC ACTGACCTGC AGGGTCGTAT CTATGGACTG 
GAGGGTGAGT TGAAGCATAT AAAGAGTTCT CTATCAAAAG CTGAAGTTGA GAAAAGACAA 
TTACAAGAGG ATCTTACAAC CCTGGAAAAG GAGAAGAATA AGCAGGAGAT CGACCTCTCC 
TTCAAACTCA AAGCAGTTCA GCAGAGTCTG GAGCAGGAGG AGGCTGAACA TAAAACCACT 
AAAGCCAGAT TGGCCGACAA CAACAAAATC AACCAATCCA TAGAGGCAAA GTCTGAGACC 
CTGAAAGACA TGGAACATAA GCTGTTGGAA GAACGATCTG CGAAGCAACA ACTGGAAAAC 
AGACTGATGC AGCTGGAGAA GGAAAACTCT GTATTAGACT GTGATTACAA ACAAGCCAAA 
CATGAGCTTC AGGAGCTCCG CTCGCTCAAA GAAAATCTCA CAGAGCAGGT GGAGGTGCTG 
AATGTGCGGG TGCAGCAGGA GACACAGAGG AAAACTCTCT GTCAAGGTGA TCTCAAAGTT 
CAGAGGCAGG AGATCAACAG CCTGCGGTCA TCAGAACAAC AGCTCAAACA AGAACTCAAC 
CATCTGCTGG AGCTCAAACT CACACTGGAG AAACAAAACC AGGAGCTGTC CAAGGAACGA 
GAAGAATCTG AAAAACAGCT GAAGGAGATG AAGGACCAGC TGGAGGCTGA GCAGTACTTC 
ACGAAACTGT ACAAGACTCA GATTCGAGAG CTGAAAGAGG AAAGCGATGA AAAGGTTAAA 
CTCTACAAAG ATGCTCAGCA GAGAATAGAG GACCTGCAGG AAGAGAGGGA CTCGTTAGCG 
TCTCAGTTGG AGGTGAGCCT GACGAAGGCT GACTCTGAAC AGTTGGCTCG CATCACCGTT 
GAAGAGCAAT ACTCGGACCT GGAGAAAGAG AAGATCATGA AGGAACTGGA GATAAAGGAC 
ATGATCGCCA GACACCGGCA GGATCTTGCA GAAAAGGACG GTACCATCAA CTCGCTGGAG 
GAGTCAAACC GTACGCTGAC TGTGGATGTT GCCAACCTGG CCAGTGAGAA AGAGGAGCTC 
AACAATAAAC TGAAGCACAT ACAGCAAAAG CTGGAGAAGA TCAAAGAAGA AGAGAAACAG 
ATGAAATCTC TCACAGTGTC GTATGAGAAG CAGATACAGG TGGAGAAAAC ACTCAAGATT 
CAGGCTATAA ACAAGCTGGC GGAGGTCATG AAGAAGACGG ACGGCAGGCC ACACCAAATC 
AACAACACAG ACATCCGCAG GAAGGAGAAG GAGATCAGAC AACTCCAGCT GCAGCTCCGC 
ACGGAGAAAG AAAAGCTTAA CCACTCCATC ATCAAATACC AGAGAGAAAT CAACGACATG 
CAGGCTATGA TAGCAGAGGA GTCTCAGGTG CGTCTGGAGA TGCAGATGTC TCTGGACAGT 
AAAGACAGTG ATATTGAGCG TCTGCGCAGT CAACTCACGT CTCTGAGCAT TCACTCTCTG 
GACACCACCA GCATCAGCAG CATCGGCAAT GACCTGGATT CTGACGAGGC ATATCCAGTG 
CGAATCACAC ACTCACACAC CTCGGAGTCT ATGTCCTTCA CATACCAGCG AAGCTCCAAG 
TCGGTGTGTA TTGATACCCG GCCCTGTCGA ATCGCTCCTC GCTTCTCCTC TGACTCTGAT 
GAAGAGGAGG ATGAGGAAGA TGAGTGGAGA CAAACACCTG CCCTTACATA TGAGACGCCT 
CCTGACCCTG AGGCTGCAGC CGCAGAGTCC GGTCTTGAAG GATGGCTCTC AATGCCTGCC 
AAAAACACCA AACGGTTTGG ATGGGATAAG AAGCTGTGCT GTGCATTCCA AGATGAAAAG 
TACCTTTATT TGGTGATGGA GTTCATGCCA GGTGGAGATC TGGTGACTCT GACCAGCAAC 
TATGACATCC CAGAGGAATG GGCTCAATTC TATACGGCAG AAGTGGTGCT GGCACTCGAC 
GCCATCCATT CTCTGGGCTT CATCCACAGA GACATCAAAC CAGACAACAT GCTGCTGGAC 
CGCAATGGAC ACCTCAAACT CGCAGACTTT GGCACATGCA TGAAGATGGA CTCGACTGGT 
TTGGTAAGCT GTGACGCTGC TGTTGGGACA CCTGATTACA TTTCTCCAGA GGTTTTAATG 
TCTCAAGGAG GGACTGGTTA TTACGGTCGC GAGTGTGACT GGTGGTCTGT TGGTGTTTTT 
ATTTATGAGC TTCTAGTGGG CGACACTCCA TTTTATTCCG AGTCTCTGGT GGGCACATAT 
GGAAAGATCA TGGATCACAA GAACAGCTTA ACTTTCCCAG ATGATATTGA AATGTCAAAG 
GATGCCAAAG ACCTCATCTG TGCCTTTCTG AGTAGCAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

rock2b ( XM_021468361.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021324036.1 Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X9, mRNA.

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CloneID	ODa150581
Clone ID Related Accession (Same CDS sequence)	XM_021468361.1
Accession Version	XM_021468361.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4041bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	rho-associated, coiled-coil containing protein kinase 2b isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007131.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCGACCG GAGCGGGGAA GAGAATAGAG AGCCGGGTGA GAAAACTAGA GACGCTGATC 
AAGAACCAGA AATCACCGTT GAACTTGGAG AGTTTGCTGG ATTCAGTAAC TGCCCTTGCC 
CTTGATCTGA ACCATCCAGC ACTGAGAAAG AACAAAAACA TAGATGCCTT CTTAAGCAGA 
TATGAAAAAG CCGTGTGCAA CCTCAAGGAA CTACAGGTGA AGCTGGATGA TTTTGATCGA 
GTCAAGCTGA TCGGTCGTGG AGCGTTTGGC GCAGTGCAGC TGGTACGGCA CAAAGTATCT 
CAACAAGTTT ATGCCATGAA GCAGCTCAGC AAGTTTGAGA TGGTCAAGCG CTCTGACTCC 
GCCTTTTTCT GGGAGGAGCG TGACATCATG GCTTTCTCCC AGAGCCCTTG GATAGTTCAG 
CTGTGCTGTG CATTCCAAGA TGAAAAGTAC CTTTATTTGG TGATGGAGTT CATGCCAGGT 
GGAGATCTGG TGACTCTGAC CAGCAACTAT GACATCCCAG AGGAATGGGC TCAATTCTAT 
ACGGCAGAAG TGGTGCTGGC ACTCGACGCC ATCCATTCTC TGGGCTTCAT CCACAGAGAC 
ATCAAACCAG ACAACATGCT GCTGGACCGC AATGGACACC TCAAACTCGC AGACTTTGGC 
ACATGCATGA AGATGGACTC GACTGGTATG GTACGCTGTG ACACTGCTGT TGGGACACCT 
GATTACATTT CTCCAGAGGT TTTAATGTCT CAAGGAGGGA CTGGTTATTA CGGTCGCGAG 
TGTGACTGGT GGTCTGTTGG TGTTTTTATT TATGAGCTTC TAGTGGGCGA CACTCCATTT 
TATTCCGAGT CTCTGGTGGG CACATATGGA AAGATCATGG ATCACAAGAA CAGCTTAACT 
TTCCCAGATG ATATTGAAAT GTCAAAGAAT GCCAAAGACC TCATCTGTGC CTTTCTGAGT 
AGCAGGGAGG TGAGGCTTGG CCGAACTGGA GTGGATGAAA TAAAATGTCA CCCCTTCTTT 
AAGAATGATC AGTGGACCTT CGACACCATA CGAGACACCA TGGCTCCAGT GGTGCCAGAG 
CTGAGTAGCG ATATTGACAC CAGTAACTTT GATGATGACA TTAAGGATGA TCCGATAGGA 
ACAGAGACGT TCCCTCCGCC CCGTGCCTTC GCAGGGAACC AGCTACCATT TGTGGGTTTC 
ACCTATTTCA GGGAGGACCA ATTGTTAACA CAACAAAATA AGTGTTCAGA GGAAGATATC 
ATTGAAGATC ATGGCTTAAA CCACACAGAG TCTAACGACC TGGAGAAGAG GCTGAAAAAG 
TTGGAGGAAA AATTCAAACA TGAGATGCAA GCAAAGGAAG AACTGGAAAA TAAATGCCGA 
ATAGCAAATC AGCGGCTGGA GAAATTGTCC AAAGATCTTG AAGAAGAAGT GAATGCGAGA 
CAGGAGGCGG AAGACAACCT GAGATCCCTG GAGAAAGAGA AAGTTTTACT GAAACACCAG 
CGTACACAGA GCGTAAGAAA AGCTGGTCTA GAGACCGACA GAAAACGGCT GCTGGAGAAT 
GAAGTAAGCA GCCTGAAAGA GCAGCTTGCC GAACTGAAGA AAAAGAACCA GATCTCTCAT 
CTGTCTGCTG AGAAGAACAT TCACCTGGAA AGACAGCTGG AGGAGGTGAG TGCCAAGCTT 
CAGGCCGAGC TGGAGGAGTC GGAGAGGCTG AAGAAGGCCC AGATCGAGGC CTTTAGACAG 
TCCCAGCAGC TGGAGCTTTC CCTCAGGGAG CTGCAGGAGC GTCTGGCCCA GCTGGAGAAC 
AGCAGGCTGG TTCTGGAGCA GGACAAACTC AGCCTGCAGA CCTCGCTAGA GCTGGAGAAA 
CGAGAGAGAA ACGCCGGCTC AGAGACCATC ACTGACCTGC AGGGTCGTAT CTATGGACTG 
GAGGGTGAGT TGAAGCATAT AAAGAGTTCT CTATCAAAAG CTGAAGTTGA GAAAAGACAA 
TTACAAGAGG ATCTTACAAC CCTGGAAAAG GAGAAGAATA AGCAGGAGAT CGACCTCTCC 
TTCAAACTCA AAGCAGTTCA GCAGAGTCTG GAGCAGGAGG AGGCTGAACA TAAAACCACT 
AAAGCCAGAT TGGCCGACAA CAACAAAATC AACCAATCCA TAGAGGCAAA GTCTGAGACC 
CTGAAAGACA TGGAACATAA GCTGTTGGAA GAACGATCTG CGAAGCAACA ACTGGAAAAC 
AGACTGATGC AGCTGGAGAA GGAAAACTCT GTATTAGACT GTGATTACAA ACAAGCCAAA 
CATGAGCTTC AGGAGCTCCG CTCGCTCAAA GAAAATCTCA CAGAGCAGGT GGAGGTGCTG 
AATGTGCGGG TGCAGCAGGA GACACAGAGG AAAACTCTCT GTCAAGGTGA TCTCAAAGTT 
CAGAGGCAGG AGATCAACAG CCTGCGGTCA TCAGAACAAC AGCTCAAACA AGAACTCAAC 
CATCTGCTGG AGCTCAAACT CACACTGGAG AAACAAAACC AGGAGCTGTC CAAGGAACGA 
GAAGAATCTG AAAAACAGCT GAAGGAGATG AAGGACCAGC TGGAGGCTGA GCAGTACTTC 
ACGAAACTGT ACAAGACTCA GATTCGAGAG CTGAAAGAGG AAAGCGATGA AAAGGTTAAA 
CTCTACAAAG ATGCTCAGCA GAGAATAGAG GACCTGCAGG AAGAGAGGGA CTCGTTAGCG 
TCTCAGTTGG AGGTGAGCCT GACGAAGGCT GACTCTGAAC AGTTGGCTCG CATCACCGTT 
GAAGAGCAAT ACTCGGACCT GGAGAAAGAG AAGATCATGA AGGAACTGGA GATAAAGGAC 
ATGATCGCCA GACACCGGCA GGATCTTGCA GAAAAGGACG GTACCATCAA CTCGCTGGAG 
GAGTCAAACC GTACGCTGAC TGTGGATGTT GCCAACCTGG CCAGTGAGAA AGAGGAGCTC 
AACAATAAAC TGAAGCACAT ACAGCAAAAG CTGGAGAAGA TCAAAGAAGA AGAGAAACAG 
ATGAAATCTC TCACAGTGTC GTATGAGAAG CAGATACAGG TGGAGAAAAC ACTCAAGATT 
CAGGCTATAA ACAAGCTGGC GGAGGTCATG AAGAAGACGG ACGGCAGGCC ACACCAAATC 
AACAACACAG ACATCCGCAG GAAGGAGAAG GAGATCAGAC AACTCCAGCT GCAGCTCCGC 
ACGGAGAAAG AAAAGCTTAA CCACTCCATC ATCAAATACC AGAGAGAAAT CAACGACATG 
CAGGCTATGA TAGCAGAGGA GTCTCAGGTG CGTCTGGAGA TGCAGATGTC TCTGGACAGT 
AAAGACAGTG ATATTGAGCG TCTGCGCAGT CAACTCACGT CTCTGAGCAT TCACTCTCTG 
GACACCACCA GCATCAGCAG CATCGGCAAT GACCTGGATT CTGACGAGGC ATATCCAGCC 
GCAGAGTCCG GTCTTGAAGG ATGGCTCTCA ATGCCTGCCA AAAACACCAA ACGGTTTGGA 
TGGGATAAGA AGCTGTGCTG TGCATTCCAA GATGAAAAGT ACCTTTATTT GGTGATGGAG 
TTCATGCCAG GTGGAGATCT GGTGACTCTG ACCAGCAACT ATGACATCCC AGAGGAATGG 
GCTCAATTCT ATACGGCAGA AGTGGTGCTG GCACTCGACG CCATCCATTC TCTGGGCTTC 
ATCCACAGAG ACATCAAACC AGACAACATG CTGCTGGACC GCAATGGACA CCTCAAACTC 
GCAGACTTTG GCACATGCAT GAAGATGGAC TCGACTGGTT TGGTAAGCTG TGACGCTGCT 
GTTGGGACAC CTGATTACAT TTCTCCAGAG GTTTTAATGT CTCAAGGAGG GACTGGTTAT 
TACGGTCGCG AGTGTGACTG GTGGTCTGTT GGTGTTTTTA TTTATGAGCT TCTAGTGGGC 
GACACTCCAT TTTATTCCGA GTCTCTGGTG GGCACATATG GAAAGATCAT GGATCACAAG 
AACAGCTTAA CTTTCCCAGA TGATATTGAA ATGTCAAAGG ATGCCAAAGA CCTCATCTGT 
GCCTTTCTGA GTAGCAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021324036.1
CDS	476..4516
Translation	MSTGAGKRIESRVRKLETLIKNQKSPLNLESLLDSVTALALDLNHPALRKNKNIDAFLSRYEKAVCNLKELQVKLDDFDRVKLIGRGAFGAVQLVRHKVSQQVYAMKQLSKFEMVKRSDSAFFWEERDIMAFSQSPWIVQLCCAFQDEKYLYLVMEFMPGGDLVTLTSNYDIPEEWAQFYTAEVVLALDAIHSLGFIHRDIKPDNMLLDRNGHLKLADFGTCMKMDSTGMVRCDTAVGTPDYISPEVLMSQGGTGYYGRECDWWSVGVFIYELLVGDTPFYSESLVGTYGKIMDHKNSLTFPDDIEMSKNAKDLICAFLSSREVRLGRTGVDEIKCHPFFKNDQWTFDTIRDTMAPVVPELSSDIDTSNFDDDIKDDPIGTETFPPPRAFAGNQLPFVGFTYFREDQLLTQQNKCSEEDIIEDHGLNHTESNDLEKRLKKLEEKFKHEMQAKEELENKCRIANQRLEKLSKDLEEEVNARQEAEDNLRSLEKEKVLLKHQRTQSVRKAGLETDRKRLLENEVSSLKEQLAELKKKNQISHLSAEKNIHLERQLEEVSAKLQAELEESERLKKAQIEAFRQSQQLELSLRELQERLAQLENSRLVLEQDKLSLQTSLELEKRERNAGSETITDLQGRIYGLEGELKHIKSSLSKAEVEKRQLQEDLTTLEKEKNKQEIDLSFKLKAVQQSLEQEEAEHKTTKARLADNNKINQSIEAKSETLKDMEHKLLEERSAKQQLENRLMQLEKENSVLDCDYKQAKHELQELRSLKENLTEQVEVLNVRVQQETQRKTLCQGDLKVQRQEINSLRSSEQQLKQELNHLLELKLTLEKQNQELSKEREESEKQLKEMKDQLEAEQYFTKLYKTQIRELKEESDEKVKLYKDAQQRIEDLQEERDSLASQLEVSLTKADSEQLARITVEEQYSDLEKEKIMKELEIKDMIARHRQDLAEKDGTINSLEESNRTLTVDVANLASEKEELNNKLKHIQQKLEKIKEEEKQMKSLTVSYEKQIQVEKTLKIQAINKLAEVMKKTDGRPHQINNTDIRRKEKEIRQLQLQLRTEKEKLNHSIIKYQREINDMQAMIAEESQVRLEMQMSLDSKDSDIERLRSQLTSLSIHSLDTTSISSIGNDLDSDEAYPAAESGLEGWLSMPAKNTKRFGWDKKLCCAFQDEKYLYLVMEFMPGGDLVTLTSNYDIPEEWAQFYTAEVVLALDAIHSLGFIHRDIKPDNMLLDRNGHLKLADFGTCMKMDSTGLVSCDAAVGTPDYISPEVLMSQGGTGYYGRECDWWSVGVFIYELLVGDTPFYSESLVGTYGKIMDHKNSLTFPDDIEMSKDAKDLICAFLSSR

Target ORF information:

RefSeq Version	XM_021468361.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio rho-associated, coiled-coil containing protein kinase 2b (rock2b), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021468361.1

ORF Insert Sequence:

ATGTCGACCG GAGCGGGGAA GAGAATAGAG AGCCGGGTGA GAAAACTAGA GACGCTGATC 
AAGAACCAGA AATCACCGTT GAACTTGGAG AGTTTGCTGG ATTCAGTAAC TGCCCTTGCC 
CTTGATCTGA ACCATCCAGC ACTGAGAAAG AACAAAAACA TAGATGCCTT CTTAAGCAGA 
TATGAAAAAG CCGTGTGCAA CCTCAAGGAA CTACAGGTGA AGCTGGATGA TTTTGATCGA 
GTCAAGCTGA TCGGTCGTGG AGCGTTTGGC GCAGTGCAGC TGGTACGGCA CAAAGTATCT 
CAACAAGTTT ATGCCATGAA GCAGCTCAGC AAGTTTGAGA TGGTCAAGCG CTCTGACTCC 
GCCTTTTTCT GGGAGGAGCG TGACATCATG GCTTTCTCCC AGAGCCCTTG GATAGTTCAG 
CTGTGCTGTG CATTCCAAGA TGAAAAGTAC CTTTATTTGG TGATGGAGTT CATGCCAGGT 
GGAGATCTGG TGACTCTGAC CAGCAACTAT GACATCCCAG AGGAATGGGC TCAATTCTAT 
ACGGCAGAAG TGGTGCTGGC ACTCGACGCC ATCCATTCTC TGGGCTTCAT CCACAGAGAC 
ATCAAACCAG ACAACATGCT GCTGGACCGC AATGGACACC TCAAACTCGC AGACTTTGGC 
ACATGCATGA AGATGGACTC GACTGGTATG GTACGCTGTG ACACTGCTGT TGGGACACCT 
GATTACATTT CTCCAGAGGT TTTAATGTCT CAAGGAGGGA CTGGTTATTA CGGTCGCGAG 
TGTGACTGGT GGTCTGTTGG TGTTTTTATT TATGAGCTTC TAGTGGGCGA CACTCCATTT 
TATTCCGAGT CTCTGGTGGG CACATATGGA AAGATCATGG ATCACAAGAA CAGCTTAACT 
TTCCCAGATG ATATTGAAAT GTCAAAGAAT GCCAAAGACC TCATCTGTGC CTTTCTGAGT 
AGCAGGGAGG TGAGGCTTGG CCGAACTGGA GTGGATGAAA TAAAATGTCA CCCCTTCTTT 
AAGAATGATC AGTGGACCTT CGACACCATA CGAGACACCA TGGCTCCAGT GGTGCCAGAG 
CTGAGTAGCG ATATTGACAC CAGTAACTTT GATGATGACA TTAAGGATGA TCCGATAGGA 
ACAGAGACGT TCCCTCCGCC CCGTGCCTTC GCAGGGAACC AGCTACCATT TGTGGGTTTC 
ACCTATTTCA GGGAGGACCA ATTGTTAACA CAACAAAATA AGTGTTCAGA GGAAGATATC 
ATTGAAGATC ATGGCTTAAA CCACACAGAG TCTAACGACC TGGAGAAGAG GCTGAAAAAG 
TTGGAGGAAA AATTCAAACA TGAGATGCAA GCAAAGGAAG AACTGGAAAA TAAATGCCGA 
ATAGCAAATC AGCGGCTGGA GAAATTGTCC AAAGATCTTG AAGAAGAAGT GAATGCGAGA 
CAGGAGGCGG AAGACAACCT GAGATCCCTG GAGAAAGAGA AAGTTTTACT GAAACACCAG 
CGTACACAGA GCGTAAGAAA AGCTGGTCTA GAGACCGACA GAAAACGGCT GCTGGAGAAT 
GAAGTAAGCA GCCTGAAAGA GCAGCTTGCC GAACTGAAGA AAAAGAACCA GATCTCTCAT 
CTGTCTGCTG AGAAGAACAT TCACCTGGAA AGACAGCTGG AGGAGGTGAG TGCCAAGCTT 
CAGGCCGAGC TGGAGGAGTC GGAGAGGCTG AAGAAGGCCC AGATCGAGGC CTTTAGACAG 
TCCCAGCAGC TGGAGCTTTC CCTCAGGGAG CTGCAGGAGC GTCTGGCCCA GCTGGAGAAC 
AGCAGGCTGG TTCTGGAGCA GGACAAACTC AGCCTGCAGA CCTCGCTAGA GCTGGAGAAA 
CGAGAGAGAA ACGCCGGCTC AGAGACCATC ACTGACCTGC AGGGTCGTAT CTATGGACTG 
GAGGGTGAGT TGAAGCATAT AAAGAGTTCT CTATCAAAAG CTGAAGTTGA GAAAAGACAA 
TTACAAGAGG ATCTTACAAC CCTGGAAAAG GAGAAGAATA AGCAGGAGAT CGACCTCTCC 
TTCAAACTCA AAGCAGTTCA GCAGAGTCTG GAGCAGGAGG AGGCTGAACA TAAAACCACT 
AAAGCCAGAT TGGCCGACAA CAACAAAATC AACCAATCCA TAGAGGCAAA GTCTGAGACC 
CTGAAAGACA TGGAACATAA GCTGTTGGAA GAACGATCTG CGAAGCAACA ACTGGAAAAC 
AGACTGATGC AGCTGGAGAA GGAAAACTCT GTATTAGACT GTGATTACAA ACAAGCCAAA 
CATGAGCTTC AGGAGCTCCG CTCGCTCAAA GAAAATCTCA CAGAGCAGGT GGAGGTGCTG 
AATGTGCGGG TGCAGCAGGA GACACAGAGG AAAACTCTCT GTCAAGGTGA TCTCAAAGTT 
CAGAGGCAGG AGATCAACAG CCTGCGGTCA TCAGAACAAC AGCTCAAACA AGAACTCAAC 
CATCTGCTGG AGCTCAAACT CACACTGGAG AAACAAAACC AGGAGCTGTC CAAGGAACGA 
GAAGAATCTG AAAAACAGCT GAAGGAGATG AAGGACCAGC TGGAGGCTGA GCAGTACTTC 
ACGAAACTGT ACAAGACTCA GATTCGAGAG CTGAAAGAGG AAAGCGATGA AAAGGTTAAA 
CTCTACAAAG ATGCTCAGCA GAGAATAGAG GACCTGCAGG AAGAGAGGGA CTCGTTAGCG 
TCTCAGTTGG AGGTGAGCCT GACGAAGGCT GACTCTGAAC AGTTGGCTCG CATCACCGTT 
GAAGAGCAAT ACTCGGACCT GGAGAAAGAG AAGATCATGA AGGAACTGGA GATAAAGGAC 
ATGATCGCCA GACACCGGCA GGATCTTGCA GAAAAGGACG GTACCATCAA CTCGCTGGAG 
GAGTCAAACC GTACGCTGAC TGTGGATGTT GCCAACCTGG CCAGTGAGAA AGAGGAGCTC 
AACAATAAAC TGAAGCACAT ACAGCAAAAG CTGGAGAAGA TCAAAGAAGA AGAGAAACAG 
ATGAAATCTC TCACAGTGTC GTATGAGAAG CAGATACAGG TGGAGAAAAC ACTCAAGATT 
CAGGCTATAA ACAAGCTGGC GGAGGTCATG AAGAAGACGG ACGGCAGGCC ACACCAAATC 
AACAACACAG ACATCCGCAG GAAGGAGAAG GAGATCAGAC AACTCCAGCT GCAGCTCCGC 
ACGGAGAAAG AAAAGCTTAA CCACTCCATC ATCAAATACC AGAGAGAAAT CAACGACATG 
CAGGCTATGA TAGCAGAGGA GTCTCAGGTG CGTCTGGAGA TGCAGATGTC TCTGGACAGT 
AAAGACAGTG ATATTGAGCG TCTGCGCAGT CAACTCACGT CTCTGAGCAT TCACTCTCTG 
GACACCACCA GCATCAGCAG CATCGGCAAT GACCTGGATT CTGACGAGGC ATATCCAGCC 
GCAGAGTCCG GTCTTGAAGG ATGGCTCTCA ATGCCTGCCA AAAACACCAA ACGGTTTGGA 
TGGGATAAGA AGCTGTGCTG TGCATTCCAA GATGAAAAGT ACCTTTATTT GGTGATGGAG 
TTCATGCCAG GTGGAGATCT GGTGACTCTG ACCAGCAACT ATGACATCCC AGAGGAATGG 
GCTCAATTCT ATACGGCAGA AGTGGTGCTG GCACTCGACG CCATCCATTC TCTGGGCTTC 
ATCCACAGAG ACATCAAACC AGACAACATG CTGCTGGACC GCAATGGACA CCTCAAACTC 
GCAGACTTTG GCACATGCAT GAAGATGGAC TCGACTGGTT TGGTAAGCTG TGACGCTGCT 
GTTGGGACAC CTGATTACAT TTCTCCAGAG GTTTTAATGT CTCAAGGAGG GACTGGTTAT 
TACGGTCGCG AGTGTGACTG GTGGTCTGTT GGTGTTTTTA TTTATGAGCT TCTAGTGGGC 
GACACTCCAT TTTATTCCGA GTCTCTGGTG GGCACATATG GAAAGATCAT GGATCACAAG 
AACAGCTTAA CTTTCCCAGA TGATATTGAA ATGTCAAAGG ATGCCAAAGA CCTCATCTGT 
GCCTTTCTGA GTAGCAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	rock2b
Entrez Gene ID	321070
Full Name	rho-associated, coiled-coil containing protein kinase 2b
Synonyms	cb64,sb:cb64,si:dkey-65b13.10,si:dkey-65b13.7
General protein information	Preferred Names rho-associated, coiled-coil containing protein kinase 2b Names rho-associated, coiled-coil containing protein kinase 2b rho-kinase 2
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

rock2b cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

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