ttk cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	ttk
Entrez Gene ID	317763
Full Name	ttk protein kinase
Synonyms	mps1,ncp
General protein information	Preferred Names ttk protein kinase Names dual specificity protein kinase Ttk mitotic checkpoint serine/threonine-protein kinase Mps1 monopolar spindle 1 monopolar spindle protein 1 nightcap protein nightcap
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_005157833.3	XP_005157890.1	dual specificity protein kinase Ttk isoform X2
XM_005157833.4	XP_005157890.1	dual specificity protein kinase Ttk isoform X2
XM_005157834.3	XP_005157891.1	dual specificity protein kinase Ttk isoform X1
XM_005157834.4	XP_005157891.1	dual specificity protein kinase Ttk isoform X1
NM_175042.2	NP_778207.1	dual specificity protein kinase Ttk

Pathways from BioSystems

Homologies

Pan troglodytes (chimpanzee)	TTK	XP_518603.2
Bos taurus (cattle)	TTK	NP_001192842.1
Danio rerio (zebrafish)	ttk	NP_778207.1
Rattus norvegicus (Norway rat)	Ttk	NP_001101642.1
Mus musculus (house mouse)	Ttk	NP_033471.2
Gallus gallus (chicken)	TTK	XP_419867.4
Homo sapiens (human)	TTK	NP_003309.2
	LOC718430	XP_001111082.2
Canis lupus familiaris (dog)	TTK	XP_005627621.1
Xenopus tropicalis (tropical clawed frog)	ttk	NP_001072599.1

Gene Ontology
Bibliography

Related articles in PubMed

Cohesin mediates Esco2-dependent transcriptional regulation in a zebrafish regenerating fin model of Roberts Syndrome.
Banerji R, Skibbens RV, Iovine MK
Biology open6(12)1802-1813(2017 Dec)

MicroRNA-133b Negatively Regulates Zebrafish Single Mauthner-Cell Axon Regeneration through Targeting
Huang R, Chen M, Yang L, Wagle M, Guo S, Hu B
Frontiers in molecular neuroscience10375(2017)

Esco2 regulates cx43 expression during skeletal regeneration in the zebrafish fin.
Banerji R, Eble DM, Iovine MK, Skibbens RV
Developmental dynamics : an official publication of the American Association of Anatomists245(1)7-21(2016 Jan)

RFX transcription factors are essential for hearing in mice.
Elkon R, Milon B, Morrison L, Shah M, Vijayakumar S, Racherla M, Leitch CC, Silipino L, Hadi S, Weiss-Gayet M, Barras E, Schmid CD, Ait-Lounis A, Barnes A, Song Y, Eisenman DJ, Eliyahu E, Frolenkov GI, Strome SE, Durand B, Zaghloul NA, Jones SM, Reith W, Hertzano R
Nature communications68549(2015 Oct)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Mps1 is a critical regulator of chromosome number in zebrafish
Title: Germ cell aneuploidy in zebrafish with mutations in the mitotic checkpoint gene mps1.

the proximal blastema comprises an essential subpopulation of the fin regenerate defined by the induction and function of Mps1
Title: Mps1 defines a proximal blastemal proliferative compartment essential for zebrafish fin regeneration.

The following ttk gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the ttk cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa01287	NM_175042.2 Latest version!	Danio rerio ttk protein kinase (ttk), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa21059	XM_005157833.4 Latest version!	Danio rerio ttk protein kinase (ttk), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa21060	XM_005157834.4 Latest version!	Danio rerio ttk protein kinase (ttk), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
View Old Accession Versions >>
ODa21059	XM_005157833.3	Danio rerio ttk protein kinase (ttk), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
ODa21060	XM_005157834.3	Danio rerio ttk protein kinase (ttk), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	12-14	$510.30 $729.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

ttk ( NM_175042.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_778207.1 Danio rerio ttk protein kinase (ttk), mRNA.

Price & Availability↑

CloneID	ODa01287
Clone ID Related Accession (Same CDS sequence)	NM_175042.2
Accession Version	NM_175042.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2952bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-08-17
Organism	Danio rerio(zebrafish)
Product	dual specificity protein kinase Ttk
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from AF488735.1. On Jun 3, 2003 this sequence version replaced NM_175042.1. Publication Note: This RefSeq record includes a subset of the publications that are available for this gene. Please see the Gene record to access additional publications. ##Evidence-Data-START## Transcript exon combination :: AF488735.1 [ECO:0000332] RNAseq introns :: mixed/partial sample support SAMEA2168446, SAMEA2168447 [ECO:0000350] ##Evidence-Data-END##

ATGGATGAAG AAGAGAGCAC AGAGCGACAG ATGCAGATTG CCATGCTGTG TCAAAAGCTG 
GCCATGATGA AGCAATTGTT CAATGAAGAT GACACGGATT ACATAAACCA GGCCATTAGC 
TCAAACTCCC CGGACACCTG CCGGACATTT CTGAGCAATC TGGAGAAGAA AGGCAATCCT 
CAAGCAGACC CCAGCCTGCT CAGCAAACTT ATGGACAGCT ACACGCGGGT CTTTTCCAGT 
ATGCCATTGG GCAAGTACAG TCAGAACGAG AGCTACGCCA AGATGCTGGT CAGATTTGCA 
GAGTTAAAAG CCATCCAGGA TGTAAATGAT GCACAGACTA GTTTCGATAT AGCGAGGTCC 
CACTGTAAAG ATTTTGCTTT CGTTCACGTC GCTTATGCTC AGTTTGAGCT TTTGCAAGGC 
AACATGAAGA AATGCACTAT GATTCTTCAG AAGGCATTTG AAATGAATGC TAAACCCAGA 
CACGTTCTGG AGGCTGCAGT GCGCAATCTG AAGACCGGCA AACGCCAGCT TCTATCGCAT 
GAAGATAAGG AGAACCTATC AGTATCTGCA CTGGACCACA CACAAGGCTC CAGAAGATCA 
GATGGGACGT GTGAACTGAA GCCCAGCAGC ACTTTTCTCC ACAGCAGTGA TCAGAAGTTC 
AGTCCTCAAG AGGAGAACGG GCCCGTGTGG AGAACAGGAT CCCAGCACAG AAGAACAGCC 
ATGGCGGAAA GAGTTCCCAT GGTGCCTCTG TCTATCCCTG AAAATGAGAC CAGTGACTCT 
GACTGTGCTC AGAAAGCAGA AGCTCCTTTT ACCCACAGCA GCGGTTTCTC CAGACAAACA 
AGCGGTTCAA GTGTCCGCTC TGCATTTTCG TTATGCTCCT CAAAGAAGGG CACTCCAGAT 
GGAGATTCCT ACAGCTTAAA CATAAAGCCA CCTGTTATCA GTCCGGATTA TCTTCGTGAA 
GACATTGAAG AGGGTCACAC CATCACAGCA CTTCTAAACA GAGCAGAAAA AAGAGAAACG 
GCCAGAACAG AAGAAACCAC TGACATCAAC CAGATCATCA GCACAAACTC GACTGAAGGT 
TGCCAGGCCT TCCTGAAAAA CCTTGAGAAG AGAGCAGACC CTCATTCAGA CGCAGCATTT 
CTCTCCAAAC TCCTGGACTG CTACTCCAAA GTGTTCGCCA GGTTTCCTTT GGCAGAACAT 
TGCAAAACTG AAAGCTATGC TCGAATGCTG GTTCGATATG CAGAGCTGAA AGGAATTGAA 
GATCCAGAAG ATGCTGCAGA TGATTTCAGT ATTGCCAGGT CACACTGTAA AGCTTTTGCT 
TTTGTGCACA TCGCACATGC TCAGTTTGAG CTCTCACGAG GTAATTCTAG GAAGAGTGTT 
TCGATTCTTC AGAAAGCGCT GAGCTCCAAC GCTCGACCAA TTGAACTGCT GCAAACTGCC 
ATCAGGAACC TTAAATCTGG GAAAACTCTT CTGCTTCCTG CTGACCTTCA GGAAAGCTCT 
GAAGCTGAAA ATGTGGAAGC ACAAAATTAC ATAAAGAGAG AAGAAAATTC AGCGAAGGCT 
CCCGAGGATC ATCAGAAACC TTTTTCCAAG GAGACTTCAT CAGAATGGAA GATTCCTGCT 
CTCATTACCA AACACACGTC TCCAGAGGAT CGGAAAGCTT CTTTAGAGCC CGTTTCCTCT 
TCATCTTCCC ATCATGCAGT GCGAACGCCT GCACCTCTTC GATTAAACCC CTCACTCAGC 
TGTCAGACGC CCAACTACAG ACAGCCAAAT CCTAACAGTT TTGTGACTCC TGTGGTGAAG 
CAGCGGCCTG TGATTGTGTC TGTTCCAGCG ACGGCTCAGA AAATGTGTCC GACTGCGCTG 
CCCTGCACGC CTCAGAGCGG TGTGTCCTAC ATCCAGCCTC CCACGCAGAC TCCTTCTTCA 
GCCTTCTCAA ATGAATCCAT AACAATTAAG GGCAAGCAGT TCTTCATTTT CAAGATGATC 
GGCAGAGGAG GATCCAGTAA GGTATATCAG GTCTTTGACC ATAAGAAGCA CGTGTACGCT 
GTCAAATATG TGAATCTGGA GGAGGCCGAT GCTCAGGCGG TGGAAAGCTA CAAGAATGAG 
ATCGAGCACT TGAATCATCT TCAGCAGTAC AGCGACCAGA TCATCAAGCT CTATGACTAT 
GAGATCACCA GCAGCTACAT CTACATGCTG ATGGAGTGCG GACACCTGGA TCTGAACACC 
TGGCTGCGCA ACCGCAAGAC CGTCAAACCG CTGGACAGGA AGGCTTACTG GAGGAACATG 
CTGGAGGCTG TGCACACCAT CCACAAACAC GGCATCGTCC ACAGTGACTT AAAGCCAGCC 
AATTTTTTGA TAGTGGATGG TTCGCTGAAG CTAATAGACT TTGGAATCGC CAACCAGATT 
CAGCCTGACG TGACCAGCAT CATGAAGGAC TCGCAGGTCG GAACTCTGAA CTACATGCCA 
CCGGAGGCCA TTAAAGACAC GTCCTCCAAT GGAAAGCCGG GGTCAAAGAT CAGTGCTAAA 
GGGGACGTGT GGTCTCTGGG CTGCATCCTG TATTGCATGA CATACGGCAA GACTCCATTC 
CAGAACATCA CCAATCAGAT CAGCAAAATC CACGCCATTA TCGACCCGTC CCACGAGATC 
GACTTCCCTG ACATTCCCGA AAAAGACCTG CTGGATGTGC TGAAGAAATG TTTAGTTCGC 
AACCCTCGAG AAAGGATTTC CATTGCAGAG CTGCTCGATC ATCCATACCT GCAGCTACAG 
CCACAACCTG CACCTGAGCC AGCAGAAACG TCCTCCAGTG ATTTTAAGAG GATCCTCAAT 
GAACTGGTGG CTCTTCAGTC TCCAAACAGT ATCGCTAGAG CTGCCAGCAA TTTAGCCATG 
ATGTGCAACA GCGGGAGGAA ACTGGATGTT TCTGAATGTG TGAAAAGCTC CAGTCAGACG 
CTGTGGAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_778207.1
CDS	112..3063
Translation	MDEEESTERQMQIAMLCQKLAMMKQLFNEDDTDYINQAISSNSPDTCRTFLSNLEKKGNPQADPSLLSKLMDSYTRVFSSMPLGKYSQNESYAKMLVRFAELKAIQDVNDAQTSFDIARSHCKDFAFVHVAYAQFELLQGNMKKCTMILQKAFEMNAKPRHVLEAAVRNLKTGKRQLLSHEDKENLSVSALDHTQGSRRSDGTCELKPSSTFLHSSDQKFSPQEENGPVWRTGSQHRRTAMAERVPMVPLSIPENETSDSDCAQKAEAPFTHSSGFSRQTSGSSVRSAFSLCSSKKGTPDGDSYSLNIKPPVISPDYLREDIEEGHTITALLNRAEKRETARTEETTDINQIISTNSTEGCQAFLKNLEKRADPHSDAAFLSKLLDCYSKVFARFPLAEHCKTESYARMLVRYAELKGIEDPEDAADDFSIARSHCKAFAFVHIAHAQFELSRGNSRKSVSILQKALSSNARPIELLQTAIRNLKSGKTLLLPADLQESSEAENVEAQNYIKREENSAKAPEDHQKPFSKETSSEWKIPALITKHTSPEDRKASLEPVSSSSSHHAVRTPAPLRLNPSLSCQTPNYRQPNPNSFVTPVVKQRPVIVSVPATAQKMCPTALPCTPQSGVSYIQPPTQTPSSAFSNESITIKGKQFFIFKMIGRGGSSKVYQVFDHKKHVYAVKYVNLEEADAQAVESYKNEIEHLNHLQQYSDQIIKLYDYEITSSYIYMLMECGHLDLNTWLRNRKTVKPLDRKAYWRNMLEAVHTIHKHGIVHSDLKPANFLIVDGSLKLIDFGIANQIQPDVTSIMKDSQVGTLNYMPPEAIKDTSSNGKPGSKISAKGDVWSLGCILYCMTYGKTPFQNITNQISKIHAIIDPSHEIDFPDIPEKDLLDVLKKCLVRNPRERISIAELLDHPYLQLQPQPAPEPAETSSSDFKRILNELVALQSPNSIARAASNLAMMCNSGRKLDVSECVKSSSQTLWK

Target ORF information:

RefSeq Version	NM_175042.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ttk protein kinase (ttk), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_175042.2

ORF Insert Sequence:

ATGGATGAAG AAGAGAGCAC AGAGCGACAG ATGCAGATTG CCATGCTGTG TCAAAAGCTG 
GCCATGATGA AGCAATTGTT CAATGAAGAT GACACGGATT ACATAAACCA GGCCATTAGC 
TCAAACTCCC CGGACACCTG CCGGACATTT CTGAGCAATC TGGAGAAGAA AGGCAATCCT 
CAAGCAGACC CCAGCCTGCT CAGCAAACTT ATGGACAGCT ACACGCGGGT CTTTTCCAGT 
ATGCCATTGG GCAAGTACAG TCAGAACGAG AGCTACGCCA AGATGCTGGT CAGATTTGCA 
GAGTTAAAAG CCATCCAGGA TGTAAATGAT GCACAGACTA GTTTCGATAT AGCGAGGTCC 
CACTGTAAAG ATTTTGCTTT CGTTCACGTC GCTTATGCTC AGTTTGAGCT TTTGCAAGGC 
AACATGAAGA AATGCACTAT GATTCTTCAG AAGGCATTTG AAATGAATGC TAAACCCAGA 
CACGTTCTGG AGGCTGCAGT GCGCAATCTG AAGACCGGCA AACGCCAGCT TCTATCGCAT 
GAAGATAAGG AGAACCTATC AGTATCTGCA CTGGACCACA CACAAGGCTC CAGAAGATCA 
GATGGGACGT GTGAACTGAA GCCCAGCAGC ACTTTTCTCC ACAGCAGTGA TCAGAAGTTC 
AGTCCTCAAG AGGAGAACGG GCCCGTGTGG AGAACAGGAT CCCAGCACAG AAGAACAGCC 
ATGGCGGAAA GAGTTCCCAT GGTGCCTCTG TCTATCCCTG AAAATGAGAC CAGTGACTCT 
GACTGTGCTC AGAAAGCAGA AGCTCCTTTT ACCCACAGCA GCGGTTTCTC CAGACAAACA 
AGCGGTTCAA GTGTCCGCTC TGCATTTTCG TTATGCTCCT CAAAGAAGGG CACTCCAGAT 
GGAGATTCCT ACAGCTTAAA CATAAAGCCA CCTGTTATCA GTCCGGATTA TCTTCGTGAA 
GACATTGAAG AGGGTCACAC CATCACAGCA CTTCTAAACA GAGCAGAAAA AAGAGAAACG 
GCCAGAACAG AAGAAACCAC TGACATCAAC CAGATCATCA GCACAAACTC GACTGAAGGT 
TGCCAGGCCT TCCTGAAAAA CCTTGAGAAG AGAGCAGACC CTCATTCAGA CGCAGCATTT 
CTCTCCAAAC TCCTGGACTG CTACTCCAAA GTGTTCGCCA GGTTTCCTTT GGCAGAACAT 
TGCAAAACTG AAAGCTATGC TCGAATGCTG GTTCGATATG CAGAGCTGAA AGGAATTGAA 
GATCCAGAAG ATGCTGCAGA TGATTTCAGT ATTGCCAGGT CACACTGTAA AGCTTTTGCT 
TTTGTGCACA TCGCACATGC TCAGTTTGAG CTCTCACGAG GTAATTCTAG GAAGAGTGTT 
TCGATTCTTC AGAAAGCGCT GAGCTCCAAC GCTCGACCAA TTGAACTGCT GCAAACTGCC 
ATCAGGAACC TTAAATCTGG GAAAACTCTT CTGCTTCCTG CTGACCTTCA GGAAAGCTCT 
GAAGCTGAAA ATGTGGAAGC ACAAAATTAC ATAAAGAGAG AAGAAAATTC AGCGAAGGCT 
CCCGAGGATC ATCAGAAACC TTTTTCCAAG GAGACTTCAT CAGAATGGAA GATTCCTGCT 
CTCATTACCA AACACACGTC TCCAGAGGAT CGGAAAGCTT CTTTAGAGCC CGTTTCCTCT 
TCATCTTCCC ATCATGCAGT GCGAACGCCT GCACCTCTTC GATTAAACCC CTCACTCAGC 
TGTCAGACGC CCAACTACAG ACAGCCAAAT CCTAACAGTT TTGTGACTCC TGTGGTGAAG 
CAGCGGCCTG TGATTGTGTC TGTTCCAGCG ACGGCTCAGA AAATGTGTCC GACTGCGCTG 
CCCTGCACGC CTCAGAGCGG TGTGTCCTAC ATCCAGCCTC CCACGCAGAC TCCTTCTTCA 
GCCTTCTCAA ATGAATCCAT AACAATTAAG GGCAAGCAGT TCTTCATTTT CAAGATGATC 
GGCAGAGGAG GATCCAGTAA GGTATATCAG GTCTTTGACC ATAAGAAGCA CGTGTACGCT 
GTCAAATATG TGAATCTGGA GGAGGCCGAT GCTCAGGCGG TGGAAAGCTA CAAGAATGAG 
ATCGAGCACT TGAATCATCT TCAGCAGTAC AGCGACCAGA TCATCAAGCT CTATGACTAT 
GAGATCACCA GCAGCTACAT CTACATGCTG ATGGAGTGCG GACACCTGGA TCTGAACACC 
TGGCTGCGCA ACCGCAAGAC CGTCAAACCG CTGGACAGGA AGGCTTACTG GAGGAACATG 
CTGGAGGCTG TGCACACCAT CCACAAACAC GGCATCGTCC ACAGTGACTT AAAGCCAGCC 
AATTTTTTGA TAGTGGATGG TTCGCTGAAG CTAATAGACT TTGGAATCGC CAACCAGATT 
CAGCCTGACG TGACCAGCAT CATGAAGGAC TCGCAGGTCG GAACTCTGAA CTACATGCCA 
CCGGAGGCCA TTAAAGACAC GTCCTCCAAT GGAAAGCCGG GGTCAAAGAT CAGTGCTAAA 
GGGGACGTGT GGTCTCTGGG CTGCATCCTG TATTGCATGA CATACGGCAA GACTCCATTC 
CAGAACATCA CCAATCAGAT CAGCAAAATC CACGCCATTA TCGACCCGTC CCACGAGATC 
GACTTCCCTG ACATTCCCGA AAAAGACCTG CTGGATGTGC TGAAGAAATG TTTAGTTCGC 
AACCCTCGAG AAAGGATTTC CATTGCAGAG CTGCTCGATC ATCCATACCT GCAGCTACAG 
CCACAACCTG CACCTGAGCC AGCAGAAACG TCCTCCAGTG ATTTTAAGAG GATCCTCAAT 
GAACTGGTGG CTCTTCAGTC TCCAAACAGT ATCGCTAGAG CTGCCAGCAA TTTAGCCATG 
ATGTGCAACA GCGGGAGGAA ACTGGATGTT TCTGAATGTG TGAAAAGCTC CAGTCAGACG 
CTGTGGAAAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ttk ( XM_005157833.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157890.1 Danio rerio ttk protein kinase (ttk), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa21059
Clone ID Related Accession (Same CDS sequence)	XM_005157833.3 , XM_005157833.4
Accession Version	XM_005157833.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	dual specificity protein kinase Ttk isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005157833.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAG AAGAGAGCAC AGAGCGACAG ATGCAGATTG CCATGCTGTG TCAAAAGCTG 
GCCATGATGA AGCAATTGTT CAATGAAGAT GACACGGATT ACATAAACCA GGCCATTAGC 
TCAAACTCCC CGGACACCTG CCGGACATTT CTGAGCAATC TGGAGAAGAA AGGCAATCCT 
CAAGCAGACC CCAGCCTGCT CAGCAAACTT ATGGACAGCT ACACGCGGGT CTTTTCCAGT 
ATGCCATTGG GCAAGTACAG TCAGAACGAG AGCTACGCCA AGATGCTGGT CAGATTTGCA 
GAGTTAAAAG CCATCCAGGA TGTAAATGAT GCACAGACTA GTTTCGATAT AGCGAGGTCC 
CACTGTAAAG ATTTTGCTTT CGTTCACGTC GCTTATGCTC AGTTTGAGCT TTTGCAAGGC 
AACATGAAGA AATGCACTAT GATTCTTCAG AAGGCATTTG AAATGAATGC TAAACCCAGA 
CACGTTCTGG AGGCTGCAGT GCGCAATCTG AAGACCGGCA AACGCCAGCT TCTATCGCAT 
GAAGATAAGG AGAACCTATC AGTATCTGCA CTGGACCACA CACAAGGCTC CAGAAGATCA 
GATGGGACGT GTGAACTGAA GCCCAGCAGC ACTTTTCTCC ACAGCAGTGA TCAGAAGTTC 
AGTCCTCAAG AGGAGAACGG GCCCGTGTGG AGAACAGGAT CCCAGCACAG AAGAACAGCC 
ATGGCGGAAA GAGTTCCCAT GGTGCCTCTG TCTATCCCTG AAAATGAGAC CAGTGACTCT 
GACTGTGCTC AGAAAGCAGA AGCTCCTTTT ACCCACAGCA GCGGTTTCTC CAGACAAACA 
AGCGGTTCAA GTGTCCGCTC TGCATTTTCG TTATGCTCCT CAAAGAAGGG CACTCCAGAT 
GGAGATTCCT ACAGCTTAAA CATAAAGCCA CCTGTTATCA GTCCAGATTA TCTTCGTGAA 
GACATTGAAG AGGGTCACAC TATCACAGCA CTTCTAAACA GAGCAGAAAA AAGAGAAACG 
GCCAGAACAG AAGAAACCAC TGACATCAAC CAGATCATCA GCACAAACTC GACTGAAGGT 
TGCCAGGCCT TCCTGAAAAA CCTTGAGAAG AGAGCAGACC CTCATTCAGA CGCAGCATTT 
CTCTCCAAAC TCCTGGACTG CTACTCCAAA GTGTTCGCCA GGTTTCCTTT GGCAGAACAT 
TGCAAAACTG AAAGCTATGC TCGAATGCTG GTTCGATATG CAGAGCTGAA AGGAATTGAA 
GATCCAGAAG ATGCTGCAGA TGATTTCAGT ATTGCCAGGT CACACTGTAA AGCTTTTGCT 
TTTGTGCACA TCGCACATGC TCAGTTTGAG CTCTCACGAG GTAATTCTAG GAAGAGTGTT 
TCGATTCTTC AGAAAGCGCT GAGCTCCAAC GCTCGACCAA TTGAACTGCT GCAAACTGCC 
ATCAGGAACC TTAAATCTGG GAAAACTCTT CTGCTTCCTG CTGAGCATCA GGAAAGCTCT 
GAAGCTGAAA ATGTGGAAGC ACAAAATGGC ATGAAGAGAG AAGAAAATCC AGTGAAGGCT 
CCCGAGGATC ATCAGAAACC TTTTTCCAAG GAGACTTCAT CAGAATGGAA GATTCCTGCT 
CTCATTACCA AACACACGTC TCCAGAGGAT CGGAAAGCTC CTGTAGAGCC CGTTTCCTCT 
TCATCTTCCC ATCATGCAGT GCGAACGCCT GCACCTCTTC GATTAAACCC CTCACTCAGC 
TGTCAGACGC CCAACTACAG ACAGCCAAAT CCTAACAGTT TTGTGACTCC TGTGGTGAAG 
CAGCGGCCTG TGATTGTGTC TGTTCCAGCG ACGGCTCAGA AAATGTGTCC GACTGCGCTG 
CCCTGCACGC CTCAGAGCGG TGTGTCCTAC ATCCAGCCTC CCACGCAGAC TCCTTCTTCA 
GCCTTCTCAA ATGAATCCAT AACAATTAAG GGCAAGCAGT TCTTCATTTT CAAGATGATC 
GGCAGAGGAG GATCCAGTAA GGTATATCAG GTCTTTGACC ATAAGAAGCA CGTGTACGCT 
GTCAAATATG TGAATCTGGA GGAGGCTGAT GCTCAGGCGG TGGAAAGCTA CAAGAATGAG 
ATCGAGCACT TGAATCATCT TCAGCAGTAC AGCGACCAGA TCATCAAGCT CTATGACTAT 
GAGATCACCA GCAGCTACAT CTACATGCTG ATGGAGTGCG GACACCTGGA TCTGAACACC 
TGGCTGCGCA ACCGCAAGAC CGTCAAACCG CTGGACAGGA AGGCTTACTG GAGGAACATG 
CTGGAGGCTG TGCACACCAT CCACAAACAC GGCATCGTCC ACAGTGACTT AAAGCCAGCC 
AATTTTTTGA TAGTGGATGG TTCGCTGAAG CTAATAGACT TTGGAATCGC CAACCAGATT 
CAGCCTGACG TGACCAGCAT CATGAAGGAC TCGCAGGTGG GAACTCTGAA CTACATGCCA 
CCGGAGGCCA TTAAAGACAC GTCCTCCAAT GGAAAGCCGG GGTCAAAGAT CAGTGCTAAA 
GGGGACGTGT GGTCTCTGGG CTGCATCCTG TATTGCATGA CATACGGCAA GACTCCATTC 
CAGAACATCA CCAATCAGAT CAGCAAAATC CACGCCATTA TCGACCCGTC CCACGAGATC 
GACTTCCCTG ACATTCCCGA AAAAGACCTG CTGGATGTGC TGAAGAAATG TTTAGTTCGC 
AACCCTCGAG AAAGGATTTC CATTGCAGAG CTGCTCGATC ATCCATACCT GCAGCTACAG 
CCACAACCTG CACCTGAGCC AGAAACGTCC TCCAGTGATT TTAAGAGGAT CCTCAATGAA 
CTGGTGGCTC TTCAGTCTCC AAACAGTATC GCTAGAGCTG CCAGCAATTT AGCCATGATG 
TGCAACAGCG GGAGGAAACT GGATGTTTCT GAATGTGTGA AAAGCTCCAG TCAGACGCTG 
TGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157890.1
CDS	109..3057
Translation	MDEEESTERQMQIAMLCQKLAMMKQLFNEDDTDYINQAISSNSPDTCRTFLSNLEKKGNPQADPSLLSKLMDSYTRVFSSMPLGKYSQNESYAKMLVRFAELKAIQDVNDAQTSFDIARSHCKDFAFVHVAYAQFELLQGNMKKCTMILQKAFEMNAKPRHVLEAAVRNLKTGKRQLLSHEDKENLSVSALDHTQGSRRSDGTCELKPSSTFLHSSDQKFSPQEENGPVWRTGSQHRRTAMAERVPMVPLSIPENETSDSDCAQKAEAPFTHSSGFSRQTSGSSVRSAFSLCSSKKGTPDGDSYSLNIKPPVISPDYLREDIEEGHTITALLNRAEKRETARTEETTDINQIISTNSTEGCQAFLKNLEKRADPHSDAAFLSKLLDCYSKVFARFPLAEHCKTESYARMLVRYAELKGIEDPEDAADDFSIARSHCKAFAFVHIAHAQFELSRGNSRKSVSILQKALSSNARPIELLQTAIRNLKSGKTLLLPAEHQESSEAENVEAQNGMKREENPVKAPEDHQKPFSKETSSEWKIPALITKHTSPEDRKAPVEPVSSSSSHHAVRTPAPLRLNPSLSCQTPNYRQPNPNSFVTPVVKQRPVIVSVPATAQKMCPTALPCTPQSGVSYIQPPTQTPSSAFSNESITIKGKQFFIFKMIGRGGSSKVYQVFDHKKHVYAVKYVNLEEADAQAVESYKNEIEHLNHLQQYSDQIIKLYDYEITSSYIYMLMECGHLDLNTWLRNRKTVKPLDRKAYWRNMLEAVHTIHKHGIVHSDLKPANFLIVDGSLKLIDFGIANQIQPDVTSIMKDSQVGTLNYMPPEAIKDTSSNGKPGSKISAKGDVWSLGCILYCMTYGKTPFQNITNQISKIHAIIDPSHEIDFPDIPEKDLLDVLKKCLVRNPRERISIAELLDHPYLQLQPQPAPEPETSSSDFKRILNELVALQSPNSIARAASNLAMMCNSGRKLDVSECVKSSSQTLWK

Target ORF information:

RefSeq Version	XM_005157833.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ttk protein kinase (ttk), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157833.3

ORF Insert Sequence:

ATGGATGAAG AAGAGAGCAC AGAGCGACAG ATGCAGATTG CCATGCTGTG TCAAAAGCTG 
GCCATGATGA AGCAATTGTT CAATGAAGAT GACACGGATT ACATAAACCA GGCCATTAGC 
TCAAACTCCC CGGACACCTG CCGGACATTT CTGAGCAATC TGGAGAAGAA AGGCAATCCT 
CAAGCAGACC CCAGCCTGCT CAGCAAACTT ATGGACAGCT ACACGCGGGT CTTTTCCAGT 
ATGCCATTGG GCAAGTACAG TCAGAACGAG AGCTACGCCA AGATGCTGGT CAGATTTGCA 
GAGTTAAAAG CCATCCAGGA TGTAAATGAT GCACAGACTA GTTTCGATAT AGCGAGGTCC 
CACTGTAAAG ATTTTGCTTT CGTTCACGTC GCTTATGCTC AGTTTGAGCT TTTGCAAGGC 
AACATGAAGA AATGCACTAT GATTCTTCAG AAGGCATTTG AAATGAATGC TAAACCCAGA 
CACGTTCTGG AGGCTGCAGT GCGCAATCTG AAGACCGGCA AACGCCAGCT TCTATCGCAT 
GAAGATAAGG AGAACCTATC AGTATCTGCA CTGGACCACA CACAAGGCTC CAGAAGATCA 
GATGGGACGT GTGAACTGAA GCCCAGCAGC ACTTTTCTCC ACAGCAGTGA TCAGAAGTTC 
AGTCCTCAAG AGGAGAACGG GCCCGTGTGG AGAACAGGAT CCCAGCACAG AAGAACAGCC 
ATGGCGGAAA GAGTTCCCAT GGTGCCTCTG TCTATCCCTG AAAATGAGAC CAGTGACTCT 
GACTGTGCTC AGAAAGCAGA AGCTCCTTTT ACCCACAGCA GCGGTTTCTC CAGACAAACA 
AGCGGTTCAA GTGTCCGCTC TGCATTTTCG TTATGCTCCT CAAAGAAGGG CACTCCAGAT 
GGAGATTCCT ACAGCTTAAA CATAAAGCCA CCTGTTATCA GTCCAGATTA TCTTCGTGAA 
GACATTGAAG AGGGTCACAC TATCACAGCA CTTCTAAACA GAGCAGAAAA AAGAGAAACG 
GCCAGAACAG AAGAAACCAC TGACATCAAC CAGATCATCA GCACAAACTC GACTGAAGGT 
TGCCAGGCCT TCCTGAAAAA CCTTGAGAAG AGAGCAGACC CTCATTCAGA CGCAGCATTT 
CTCTCCAAAC TCCTGGACTG CTACTCCAAA GTGTTCGCCA GGTTTCCTTT GGCAGAACAT 
TGCAAAACTG AAAGCTATGC TCGAATGCTG GTTCGATATG CAGAGCTGAA AGGAATTGAA 
GATCCAGAAG ATGCTGCAGA TGATTTCAGT ATTGCCAGGT CACACTGTAA AGCTTTTGCT 
TTTGTGCACA TCGCACATGC TCAGTTTGAG CTCTCACGAG GTAATTCTAG GAAGAGTGTT 
TCGATTCTTC AGAAAGCGCT GAGCTCCAAC GCTCGACCAA TTGAACTGCT GCAAACTGCC 
ATCAGGAACC TTAAATCTGG GAAAACTCTT CTGCTTCCTG CTGAGCATCA GGAAAGCTCT 
GAAGCTGAAA ATGTGGAAGC ACAAAATGGC ATGAAGAGAG AAGAAAATCC AGTGAAGGCT 
CCCGAGGATC ATCAGAAACC TTTTTCCAAG GAGACTTCAT CAGAATGGAA GATTCCTGCT 
CTCATTACCA AACACACGTC TCCAGAGGAT CGGAAAGCTC CTGTAGAGCC CGTTTCCTCT 
TCATCTTCCC ATCATGCAGT GCGAACGCCT GCACCTCTTC GATTAAACCC CTCACTCAGC 
TGTCAGACGC CCAACTACAG ACAGCCAAAT CCTAACAGTT TTGTGACTCC TGTGGTGAAG 
CAGCGGCCTG TGATTGTGTC TGTTCCAGCG ACGGCTCAGA AAATGTGTCC GACTGCGCTG 
CCCTGCACGC CTCAGAGCGG TGTGTCCTAC ATCCAGCCTC CCACGCAGAC TCCTTCTTCA 
GCCTTCTCAA ATGAATCCAT AACAATTAAG GGCAAGCAGT TCTTCATTTT CAAGATGATC 
GGCAGAGGAG GATCCAGTAA GGTATATCAG GTCTTTGACC ATAAGAAGCA CGTGTACGCT 
GTCAAATATG TGAATCTGGA GGAGGCTGAT GCTCAGGCGG TGGAAAGCTA CAAGAATGAG 
ATCGAGCACT TGAATCATCT TCAGCAGTAC AGCGACCAGA TCATCAAGCT CTATGACTAT 
GAGATCACCA GCAGCTACAT CTACATGCTG ATGGAGTGCG GACACCTGGA TCTGAACACC 
TGGCTGCGCA ACCGCAAGAC CGTCAAACCG CTGGACAGGA AGGCTTACTG GAGGAACATG 
CTGGAGGCTG TGCACACCAT CCACAAACAC GGCATCGTCC ACAGTGACTT AAAGCCAGCC 
AATTTTTTGA TAGTGGATGG TTCGCTGAAG CTAATAGACT TTGGAATCGC CAACCAGATT 
CAGCCTGACG TGACCAGCAT CATGAAGGAC TCGCAGGTGG GAACTCTGAA CTACATGCCA 
CCGGAGGCCA TTAAAGACAC GTCCTCCAAT GGAAAGCCGG GGTCAAAGAT CAGTGCTAAA 
GGGGACGTGT GGTCTCTGGG CTGCATCCTG TATTGCATGA CATACGGCAA GACTCCATTC 
CAGAACATCA CCAATCAGAT CAGCAAAATC CACGCCATTA TCGACCCGTC CCACGAGATC 
GACTTCCCTG ACATTCCCGA AAAAGACCTG CTGGATGTGC TGAAGAAATG TTTAGTTCGC 
AACCCTCGAG AAAGGATTTC CATTGCAGAG CTGCTCGATC ATCCATACCT GCAGCTACAG 
CCACAACCTG CACCTGAGCC AGAAACGTCC TCCAGTGATT TTAAGAGGAT CCTCAATGAA 
CTGGTGGCTC TTCAGTCTCC AAACAGTATC GCTAGAGCTG CCAGCAATTT AGCCATGATG 
TGCAACAGCG GGAGGAAACT GGATGTTTCT GAATGTGTGA AAAGCTCCAG TCAGACGCTG 
TGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ttk ( XM_005157833.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157890.1 Danio rerio ttk protein kinase (ttk), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa21059
Clone ID Related Accession (Same CDS sequence)	XM_005157833.3 , XM_005157833.4
Accession Version	XM_005157833.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	dual specificity protein kinase Ttk isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005157833.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAG AAGAGAGCAC AGAGCGACAG ATGCAGATTG CCATGCTGTG TCAAAAGCTG 
GCCATGATGA AGCAATTGTT CAATGAAGAT GACACGGATT ACATAAACCA GGCCATTAGC 
TCAAACTCCC CGGACACCTG CCGGACATTT CTGAGCAATC TGGAGAAGAA AGGCAATCCT 
CAAGCAGACC CCAGCCTGCT CAGCAAACTT ATGGACAGCT ACACGCGGGT CTTTTCCAGT 
ATGCCATTGG GCAAGTACAG TCAGAACGAG AGCTACGCCA AGATGCTGGT CAGATTTGCA 
GAGTTAAAAG CCATCCAGGA TGTAAATGAT GCACAGACTA GTTTCGATAT AGCGAGGTCC 
CACTGTAAAG ATTTTGCTTT CGTTCACGTC GCTTATGCTC AGTTTGAGCT TTTGCAAGGC 
AACATGAAGA AATGCACTAT GATTCTTCAG AAGGCATTTG AAATGAATGC TAAACCCAGA 
CACGTTCTGG AGGCTGCAGT GCGCAATCTG AAGACCGGCA AACGCCAGCT TCTATCGCAT 
GAAGATAAGG AGAACCTATC AGTATCTGCA CTGGACCACA CACAAGGCTC CAGAAGATCA 
GATGGGACGT GTGAACTGAA GCCCAGCAGC ACTTTTCTCC ACAGCAGTGA TCAGAAGTTC 
AGTCCTCAAG AGGAGAACGG GCCCGTGTGG AGAACAGGAT CCCAGCACAG AAGAACAGCC 
ATGGCGGAAA GAGTTCCCAT GGTGCCTCTG TCTATCCCTG AAAATGAGAC CAGTGACTCT 
GACTGTGCTC AGAAAGCAGA AGCTCCTTTT ACCCACAGCA GCGGTTTCTC CAGACAAACA 
AGCGGTTCAA GTGTCCGCTC TGCATTTTCG TTATGCTCCT CAAAGAAGGG CACTCCAGAT 
GGAGATTCCT ACAGCTTAAA CATAAAGCCA CCTGTTATCA GTCCAGATTA TCTTCGTGAA 
GACATTGAAG AGGGTCACAC TATCACAGCA CTTCTAAACA GAGCAGAAAA AAGAGAAACG 
GCCAGAACAG AAGAAACCAC TGACATCAAC CAGATCATCA GCACAAACTC GACTGAAGGT 
TGCCAGGCCT TCCTGAAAAA CCTTGAGAAG AGAGCAGACC CTCATTCAGA CGCAGCATTT 
CTCTCCAAAC TCCTGGACTG CTACTCCAAA GTGTTCGCCA GGTTTCCTTT GGCAGAACAT 
TGCAAAACTG AAAGCTATGC TCGAATGCTG GTTCGATATG CAGAGCTGAA AGGAATTGAA 
GATCCAGAAG ATGCTGCAGA TGATTTCAGT ATTGCCAGGT CACACTGTAA AGCTTTTGCT 
TTTGTGCACA TCGCACATGC TCAGTTTGAG CTCTCACGAG GTAATTCTAG GAAGAGTGTT 
TCGATTCTTC AGAAAGCGCT GAGCTCCAAC GCTCGACCAA TTGAACTGCT GCAAACTGCC 
ATCAGGAACC TTAAATCTGG GAAAACTCTT CTGCTTCCTG CTGAGCATCA GGAAAGCTCT 
GAAGCTGAAA ATGTGGAAGC ACAAAATGGC ATGAAGAGAG AAGAAAATCC AGTGAAGGCT 
CCCGAGGATC ATCAGAAACC TTTTTCCAAG GAGACTTCAT CAGAATGGAA GATTCCTGCT 
CTCATTACCA AACACACGTC TCCAGAGGAT CGGAAAGCTC CTGTAGAGCC CGTTTCCTCT 
TCATCTTCCC ATCATGCAGT GCGAACGCCT GCACCTCTTC GATTAAACCC CTCACTCAGC 
TGTCAGACGC CCAACTACAG ACAGCCAAAT CCTAACAGTT TTGTGACTCC TGTGGTGAAG 
CAGCGGCCTG TGATTGTGTC TGTTCCAGCG ACGGCTCAGA AAATGTGTCC GACTGCGCTG 
CCCTGCACGC CTCAGAGCGG TGTGTCCTAC ATCCAGCCTC CCACGCAGAC TCCTTCTTCA 
GCCTTCTCAA ATGAATCCAT AACAATTAAG GGCAAGCAGT TCTTCATTTT CAAGATGATC 
GGCAGAGGAG GATCCAGTAA GGTATATCAG GTCTTTGACC ATAAGAAGCA CGTGTACGCT 
GTCAAATATG TGAATCTGGA GGAGGCTGAT GCTCAGGCGG TGGAAAGCTA CAAGAATGAG 
ATCGAGCACT TGAATCATCT TCAGCAGTAC AGCGACCAGA TCATCAAGCT CTATGACTAT 
GAGATCACCA GCAGCTACAT CTACATGCTG ATGGAGTGCG GACACCTGGA TCTGAACACC 
TGGCTGCGCA ACCGCAAGAC CGTCAAACCG CTGGACAGGA AGGCTTACTG GAGGAACATG 
CTGGAGGCTG TGCACACCAT CCACAAACAC GGCATCGTCC ACAGTGACTT AAAGCCAGCC 
AATTTTTTGA TAGTGGATGG TTCGCTGAAG CTAATAGACT TTGGAATCGC CAACCAGATT 
CAGCCTGACG TGACCAGCAT CATGAAGGAC TCGCAGGTGG GAACTCTGAA CTACATGCCA 
CCGGAGGCCA TTAAAGACAC GTCCTCCAAT GGAAAGCCGG GGTCAAAGAT CAGTGCTAAA 
GGGGACGTGT GGTCTCTGGG CTGCATCCTG TATTGCATGA CATACGGCAA GACTCCATTC 
CAGAACATCA CCAATCAGAT CAGCAAAATC CACGCCATTA TCGACCCGTC CCACGAGATC 
GACTTCCCTG ACATTCCCGA AAAAGACCTG CTGGATGTGC TGAAGAAATG TTTAGTTCGC 
AACCCTCGAG AAAGGATTTC CATTGCAGAG CTGCTCGATC ATCCATACCT GCAGCTACAG 
CCACAACCTG CACCTGAGCC AGAAACGTCC TCCAGTGATT TTAAGAGGAT CCTCAATGAA 
CTGGTGGCTC TTCAGTCTCC AAACAGTATC GCTAGAGCTG CCAGCAATTT AGCCATGATG 
TGCAACAGCG GGAGGAAACT GGATGTTTCT GAATGTGTGA AAAGCTCCAG TCAGACGCTG 
TGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157890.1
CDS	121..3069
Translation	MDEEESTERQMQIAMLCQKLAMMKQLFNEDDTDYINQAISSNSPDTCRTFLSNLEKKGNPQADPSLLSKLMDSYTRVFSSMPLGKYSQNESYAKMLVRFAELKAIQDVNDAQTSFDIARSHCKDFAFVHVAYAQFELLQGNMKKCTMILQKAFEMNAKPRHVLEAAVRNLKTGKRQLLSHEDKENLSVSALDHTQGSRRSDGTCELKPSSTFLHSSDQKFSPQEENGPVWRTGSQHRRTAMAERVPMVPLSIPENETSDSDCAQKAEAPFTHSSGFSRQTSGSSVRSAFSLCSSKKGTPDGDSYSLNIKPPVISPDYLREDIEEGHTITALLNRAEKRETARTEETTDINQIISTNSTEGCQAFLKNLEKRADPHSDAAFLSKLLDCYSKVFARFPLAEHCKTESYARMLVRYAELKGIEDPEDAADDFSIARSHCKAFAFVHIAHAQFELSRGNSRKSVSILQKALSSNARPIELLQTAIRNLKSGKTLLLPAEHQESSEAENVEAQNGMKREENPVKAPEDHQKPFSKETSSEWKIPALITKHTSPEDRKAPVEPVSSSSSHHAVRTPAPLRLNPSLSCQTPNYRQPNPNSFVTPVVKQRPVIVSVPATAQKMCPTALPCTPQSGVSYIQPPTQTPSSAFSNESITIKGKQFFIFKMIGRGGSSKVYQVFDHKKHVYAVKYVNLEEADAQAVESYKNEIEHLNHLQQYSDQIIKLYDYEITSSYIYMLMECGHLDLNTWLRNRKTVKPLDRKAYWRNMLEAVHTIHKHGIVHSDLKPANFLIVDGSLKLIDFGIANQIQPDVTSIMKDSQVGTLNYMPPEAIKDTSSNGKPGSKISAKGDVWSLGCILYCMTYGKTPFQNITNQISKIHAIIDPSHEIDFPDIPEKDLLDVLKKCLVRNPRERISIAELLDHPYLQLQPQPAPEPETSSSDFKRILNELVALQSPNSIARAASNLAMMCNSGRKLDVSECVKSSSQTLWK

Target ORF information:

RefSeq Version	XM_005157833.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ttk protein kinase (ttk), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157833.4

ORF Insert Sequence:

ATGGATGAAG AAGAGAGCAC AGAGCGACAG ATGCAGATTG CCATGCTGTG TCAAAAGCTG 
GCCATGATGA AGCAATTGTT CAATGAAGAT GACACGGATT ACATAAACCA GGCCATTAGC 
TCAAACTCCC CGGACACCTG CCGGACATTT CTGAGCAATC TGGAGAAGAA AGGCAATCCT 
CAAGCAGACC CCAGCCTGCT CAGCAAACTT ATGGACAGCT ACACGCGGGT CTTTTCCAGT 
ATGCCATTGG GCAAGTACAG TCAGAACGAG AGCTACGCCA AGATGCTGGT CAGATTTGCA 
GAGTTAAAAG CCATCCAGGA TGTAAATGAT GCACAGACTA GTTTCGATAT AGCGAGGTCC 
CACTGTAAAG ATTTTGCTTT CGTTCACGTC GCTTATGCTC AGTTTGAGCT TTTGCAAGGC 
AACATGAAGA AATGCACTAT GATTCTTCAG AAGGCATTTG AAATGAATGC TAAACCCAGA 
CACGTTCTGG AGGCTGCAGT GCGCAATCTG AAGACCGGCA AACGCCAGCT TCTATCGCAT 
GAAGATAAGG AGAACCTATC AGTATCTGCA CTGGACCACA CACAAGGCTC CAGAAGATCA 
GATGGGACGT GTGAACTGAA GCCCAGCAGC ACTTTTCTCC ACAGCAGTGA TCAGAAGTTC 
AGTCCTCAAG AGGAGAACGG GCCCGTGTGG AGAACAGGAT CCCAGCACAG AAGAACAGCC 
ATGGCGGAAA GAGTTCCCAT GGTGCCTCTG TCTATCCCTG AAAATGAGAC CAGTGACTCT 
GACTGTGCTC AGAAAGCAGA AGCTCCTTTT ACCCACAGCA GCGGTTTCTC CAGACAAACA 
AGCGGTTCAA GTGTCCGCTC TGCATTTTCG TTATGCTCCT CAAAGAAGGG CACTCCAGAT 
GGAGATTCCT ACAGCTTAAA CATAAAGCCA CCTGTTATCA GTCCAGATTA TCTTCGTGAA 
GACATTGAAG AGGGTCACAC TATCACAGCA CTTCTAAACA GAGCAGAAAA AAGAGAAACG 
GCCAGAACAG AAGAAACCAC TGACATCAAC CAGATCATCA GCACAAACTC GACTGAAGGT 
TGCCAGGCCT TCCTGAAAAA CCTTGAGAAG AGAGCAGACC CTCATTCAGA CGCAGCATTT 
CTCTCCAAAC TCCTGGACTG CTACTCCAAA GTGTTCGCCA GGTTTCCTTT GGCAGAACAT 
TGCAAAACTG AAAGCTATGC TCGAATGCTG GTTCGATATG CAGAGCTGAA AGGAATTGAA 
GATCCAGAAG ATGCTGCAGA TGATTTCAGT ATTGCCAGGT CACACTGTAA AGCTTTTGCT 
TTTGTGCACA TCGCACATGC TCAGTTTGAG CTCTCACGAG GTAATTCTAG GAAGAGTGTT 
TCGATTCTTC AGAAAGCGCT GAGCTCCAAC GCTCGACCAA TTGAACTGCT GCAAACTGCC 
ATCAGGAACC TTAAATCTGG GAAAACTCTT CTGCTTCCTG CTGAGCATCA GGAAAGCTCT 
GAAGCTGAAA ATGTGGAAGC ACAAAATGGC ATGAAGAGAG AAGAAAATCC AGTGAAGGCT 
CCCGAGGATC ATCAGAAACC TTTTTCCAAG GAGACTTCAT CAGAATGGAA GATTCCTGCT 
CTCATTACCA AACACACGTC TCCAGAGGAT CGGAAAGCTC CTGTAGAGCC CGTTTCCTCT 
TCATCTTCCC ATCATGCAGT GCGAACGCCT GCACCTCTTC GATTAAACCC CTCACTCAGC 
TGTCAGACGC CCAACTACAG ACAGCCAAAT CCTAACAGTT TTGTGACTCC TGTGGTGAAG 
CAGCGGCCTG TGATTGTGTC TGTTCCAGCG ACGGCTCAGA AAATGTGTCC GACTGCGCTG 
CCCTGCACGC CTCAGAGCGG TGTGTCCTAC ATCCAGCCTC CCACGCAGAC TCCTTCTTCA 
GCCTTCTCAA ATGAATCCAT AACAATTAAG GGCAAGCAGT TCTTCATTTT CAAGATGATC 
GGCAGAGGAG GATCCAGTAA GGTATATCAG GTCTTTGACC ATAAGAAGCA CGTGTACGCT 
GTCAAATATG TGAATCTGGA GGAGGCTGAT GCTCAGGCGG TGGAAAGCTA CAAGAATGAG 
ATCGAGCACT TGAATCATCT TCAGCAGTAC AGCGACCAGA TCATCAAGCT CTATGACTAT 
GAGATCACCA GCAGCTACAT CTACATGCTG ATGGAGTGCG GACACCTGGA TCTGAACACC 
TGGCTGCGCA ACCGCAAGAC CGTCAAACCG CTGGACAGGA AGGCTTACTG GAGGAACATG 
CTGGAGGCTG TGCACACCAT CCACAAACAC GGCATCGTCC ACAGTGACTT AAAGCCAGCC 
AATTTTTTGA TAGTGGATGG TTCGCTGAAG CTAATAGACT TTGGAATCGC CAACCAGATT 
CAGCCTGACG TGACCAGCAT CATGAAGGAC TCGCAGGTGG GAACTCTGAA CTACATGCCA 
CCGGAGGCCA TTAAAGACAC GTCCTCCAAT GGAAAGCCGG GGTCAAAGAT CAGTGCTAAA 
GGGGACGTGT GGTCTCTGGG CTGCATCCTG TATTGCATGA CATACGGCAA GACTCCATTC 
CAGAACATCA CCAATCAGAT CAGCAAAATC CACGCCATTA TCGACCCGTC CCACGAGATC 
GACTTCCCTG ACATTCCCGA AAAAGACCTG CTGGATGTGC TGAAGAAATG TTTAGTTCGC 
AACCCTCGAG AAAGGATTTC CATTGCAGAG CTGCTCGATC ATCCATACCT GCAGCTACAG 
CCACAACCTG CACCTGAGCC AGAAACGTCC TCCAGTGATT TTAAGAGGAT CCTCAATGAA 
CTGGTGGCTC TTCAGTCTCC AAACAGTATC GCTAGAGCTG CCAGCAATTT AGCCATGATG 
TGCAACAGCG GGAGGAAACT GGATGTTTCT GAATGTGTGA AAAGCTCCAG TCAGACGCTG 
TGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ttk ( XM_005157834.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157891.1 Danio rerio ttk protein kinase (ttk), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa21060
Clone ID Related Accession (Same CDS sequence)	XM_005157834.3 , XM_005157834.4
Accession Version	XM_005157834.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	dual specificity protein kinase Ttk isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005157834.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAG AAGAGAGCAC AGAGCGACAG ATGCAGATTG CCATGCTGTG TCAAAAGCTG 
GCCATGATGA AGCAATTGTT CAATGAAGAT GACACGGATT ACATAAACCA GGCCATTAGC 
TCAAACTCCC CGGACACCTG CCGGACATTT CTGAGCAATC TGGAGAAGAA AGGCAATCCT 
CAAGCAGACC CCAGCCTGCT CAGCAAACTT ATGGACAGCT ACACGCGGGT CTTTTCCAGT 
ATGCCATTGG GCAAGTACAG TCAGAACGAG AGCTACGCCA AGATGCTGGT CAGATTTGCA 
GAGTTAAAAG CCATCCAGGA TGTAAATGAT GCACAGACTA GTTTCGATAT AGCGAGGTCC 
CACTGTAAAG ATTTTGCTTT CGTTCACGTC GCTTATGCTC AGTTTGAGCT TTTGCAAGGC 
AACATGAAGA AATGCACTAT GATTCTTCAG AAGGCATTTG AAATGAATGC TAAACCCAGA 
CACGTTCTGG AGGCTGCAGT GCGCAATCTG AAGACCGGCA AACGCCAGCT TCTATCGCAT 
GAAGATAAGG AGAACCTATC AGTATCTGCA CTGGACCACA CACAAGGCTC CAGAAGATCA 
GATGGGACGT GTGAACTGAA GCCCAGCAGC ACTTTTCTCC ACAGTGATCA GAAGTTCAGT 
CCTCAAGAGG AGAACGGGCC CGTGTGGAGA ACAGGATCCC AGCACAGAAG AACAGCCATG 
GCGGAAAGAG TTCCCATGGT GCCTCTGTCT ATCCCTGAAA ATGAGACCAG TGACTCTGAC 
TGTGCTCAGA AAGCAGAAGC TCCTTTTACC CACAGCAGCG GTTTCTCCAG ACAAACAAGC 
GGTTCAAGTG TCCGCTCTGC ATTTTCGTTA TGCTCCTCAA AGAAGGGCAC TCCAGATGGA 
GATTCCTACA GCTTAAACAT AAAGCCACCT GTTATCAGTC CAGATTATCT TCGTGAAGAC 
ATTGAAGAGG GTCACACTAT CACAGCACTT CTAAACAGAG CAGAAAAAAG AGAAACGGCC 
AGAACAGAAG AAACCACTGA CATCAACCAG ATCATCAGCA CAAACTCGAC TGAAGGTTGC 
CAGGCCTTCC TGAAAAACCT TGAGAAGAGA GCAGACCCTC ATTCAGACGC AGCATTTCTC 
TCCAAACTCC TGGACTGCTA CTCCAAAGTG TTCGCCAGGT TTCCTTTGGC AGAACATTGC 
AAAACTGAAA GCTATGCTCG AATGCTGGTT CGATATGCAG AGCTGAAAGG AATTGAAGAT 
CCAGAAGATG CTGCAGATGA TTTCAGTATT GCCAGGTCAC ACTGTAAAGC TTTTGCTTTT 
GTGCACATCG CACATGCTCA GTTTGAGCTC TCACGAGGTA ATTCTAGGAA GAGTGTTTCG 
ATTCTTCAGA AAGCGCTGAG CTCCAACGCT CGACCAATTG AACTGCTGCA AACTGCCATC 
AGGAACCTTA AATCTGGGAA AACTCTTCTG CTTCCTGCTG AGCATCAGGA AAGCTCTGAA 
GCTGAAAATG TGGAAGCACA AAATGGCATG AAGAGAGAAG AAAATCCAGT GAAGGCTCCC 
GAGGATCATC AGAAACCTTT TTCCAAGGAG ACTTCATCAG AATGGAAGAT TCCTGCTCTC 
ATTACCAAAC ACACGTCTCC AGAGGATCGG AAAGCTCCTG TAGAGCCCGT TTCCTCTTCA 
TCTTCCCATC ATGCAGTGCG AACGCCTGCA CCTCTTCGAT TAAACCCCTC ACTCAGCTGT 
CAGACGCCCA ACTACAGACA GCCAAATCCT AACAGTTTTG TGACTCCTGT GGTGAAGCAG 
CGGCCTGTGA TTGTGTCTGT TCCAGCGACG GCTCAGAAAA TGTGTCCGAC TGCGCTGCCC 
TGCACGCCTC AGAGCGGTGT GTCCTACATC CAGCCTCCCA CGCAGACTCC TTCTTCAGCC 
TTCTCAAATG AATCCATAAC AATTAAGGGC AAGCAGTTCT TCATTTTCAA GATGATCGGC 
AGAGGAGGAT CCAGTAAGGT ATATCAGGTC TTTGACCATA AGAAGCACGT GTACGCTGTC 
AAATATGTGA ATCTGGAGGA GGCTGATGCT CAGGCGGTGG AAAGCTACAA GAATGAGATC 
GAGCACTTGA ATCATCTTCA GCAGTACAGC GACCAGATCA TCAAGCTCTA TGACTATGAG 
ATCACCAGCA GCTACATCTA CATGCTGATG GAGTGCGGAC ACCTGGATCT GAACACCTGG 
CTGCGCAACC GCAAGACCGT CAAACCGCTG GACAGGAAGG CTTACTGGAG GAACATGCTG 
GAGGCTGTGC ACACCATCCA CAAACACGGC ATCGTCCACA GTGACTTAAA GCCAGCCAAT 
TTTTTGATAG TGGATGGTTC GCTGAAGCTA ATAGACTTTG GAATCGCCAA CCAGATTCAG 
CCTGACGTGA CCAGCATCAT GAAGGACTCG CAGGTGGGAA CTCTGAACTA CATGCCACCG 
GAGGCCATTA AAGACACGTC CTCCAATGGA AAGCCGGGGT CAAAGATCAG TGCTAAAGGG 
GACGTGTGGT CTCTGGGCTG CATCCTGTAT TGCATGACAT ACGGCAAGAC TCCATTCCAG 
AACATCACCA ATCAGATCAG CAAAATCCAC GCCATTATCG ACCCGTCCCA CGAGATCGAC 
TTCCCTGACA TTCCCGAAAA AGACCTGCTG GATGTGCTGA AGAAATGTTT AGTTCGCAAC 
CCTCGAGAAA GGATTTCCAT TGCAGAGCTG CTCGATCATC CATACCTGCA GCTACAGCCA 
CAACCTGCAC CTGAGCCAGC AGAAACGTCC TCCAGTGATT TTAAGAGGAT CCTCAATGAA 
CTGGTGGCTC TTCAGTCTCC AAACAGTATC GCTAGAGCTG CCAGCAATTT AGCCATGATG 
TGCAACAGCG GGAGGAAACT GGATGTTTCT GAATGTGTGA AAAGCTCCAG TCAGACGCTG 
TGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157891.1
CDS	104..3052
Translation	MDEEESTERQMQIAMLCQKLAMMKQLFNEDDTDYINQAISSNSPDTCRTFLSNLEKKGNPQADPSLLSKLMDSYTRVFSSMPLGKYSQNESYAKMLVRFAELKAIQDVNDAQTSFDIARSHCKDFAFVHVAYAQFELLQGNMKKCTMILQKAFEMNAKPRHVLEAAVRNLKTGKRQLLSHEDKENLSVSALDHTQGSRRSDGTCELKPSSTFLHSDQKFSPQEENGPVWRTGSQHRRTAMAERVPMVPLSIPENETSDSDCAQKAEAPFTHSSGFSRQTSGSSVRSAFSLCSSKKGTPDGDSYSLNIKPPVISPDYLREDIEEGHTITALLNRAEKRETARTEETTDINQIISTNSTEGCQAFLKNLEKRADPHSDAAFLSKLLDCYSKVFARFPLAEHCKTESYARMLVRYAELKGIEDPEDAADDFSIARSHCKAFAFVHIAHAQFELSRGNSRKSVSILQKALSSNARPIELLQTAIRNLKSGKTLLLPAEHQESSEAENVEAQNGMKREENPVKAPEDHQKPFSKETSSEWKIPALITKHTSPEDRKAPVEPVSSSSSHHAVRTPAPLRLNPSLSCQTPNYRQPNPNSFVTPVVKQRPVIVSVPATAQKMCPTALPCTPQSGVSYIQPPTQTPSSAFSNESITIKGKQFFIFKMIGRGGSSKVYQVFDHKKHVYAVKYVNLEEADAQAVESYKNEIEHLNHLQQYSDQIIKLYDYEITSSYIYMLMECGHLDLNTWLRNRKTVKPLDRKAYWRNMLEAVHTIHKHGIVHSDLKPANFLIVDGSLKLIDFGIANQIQPDVTSIMKDSQVGTLNYMPPEAIKDTSSNGKPGSKISAKGDVWSLGCILYCMTYGKTPFQNITNQISKIHAIIDPSHEIDFPDIPEKDLLDVLKKCLVRNPRERISIAELLDHPYLQLQPQPAPEPAETSSSDFKRILNELVALQSPNSIARAASNLAMMCNSGRKLDVSECVKSSSQTLWK

Target ORF information:

RefSeq Version	XM_005157834.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio ttk protein kinase (ttk), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157834.3

ORF Insert Sequence:

ATGGATGAAG AAGAGAGCAC AGAGCGACAG ATGCAGATTG CCATGCTGTG TCAAAAGCTG 
GCCATGATGA AGCAATTGTT CAATGAAGAT GACACGGATT ACATAAACCA GGCCATTAGC 
TCAAACTCCC CGGACACCTG CCGGACATTT CTGAGCAATC TGGAGAAGAA AGGCAATCCT 
CAAGCAGACC CCAGCCTGCT CAGCAAACTT ATGGACAGCT ACACGCGGGT CTTTTCCAGT 
ATGCCATTGG GCAAGTACAG TCAGAACGAG AGCTACGCCA AGATGCTGGT CAGATTTGCA 
GAGTTAAAAG CCATCCAGGA TGTAAATGAT GCACAGACTA GTTTCGATAT AGCGAGGTCC 
CACTGTAAAG ATTTTGCTTT CGTTCACGTC GCTTATGCTC AGTTTGAGCT TTTGCAAGGC 
AACATGAAGA AATGCACTAT GATTCTTCAG AAGGCATTTG AAATGAATGC TAAACCCAGA 
CACGTTCTGG AGGCTGCAGT GCGCAATCTG AAGACCGGCA AACGCCAGCT TCTATCGCAT 
GAAGATAAGG AGAACCTATC AGTATCTGCA CTGGACCACA CACAAGGCTC CAGAAGATCA 
GATGGGACGT GTGAACTGAA GCCCAGCAGC ACTTTTCTCC ACAGTGATCA GAAGTTCAGT 
CCTCAAGAGG AGAACGGGCC CGTGTGGAGA ACAGGATCCC AGCACAGAAG AACAGCCATG 
GCGGAAAGAG TTCCCATGGT GCCTCTGTCT ATCCCTGAAA ATGAGACCAG TGACTCTGAC 
TGTGCTCAGA AAGCAGAAGC TCCTTTTACC CACAGCAGCG GTTTCTCCAG ACAAACAAGC 
GGTTCAAGTG TCCGCTCTGC ATTTTCGTTA TGCTCCTCAA AGAAGGGCAC TCCAGATGGA 
GATTCCTACA GCTTAAACAT AAAGCCACCT GTTATCAGTC CAGATTATCT TCGTGAAGAC 
ATTGAAGAGG GTCACACTAT CACAGCACTT CTAAACAGAG CAGAAAAAAG AGAAACGGCC 
AGAACAGAAG AAACCACTGA CATCAACCAG ATCATCAGCA CAAACTCGAC TGAAGGTTGC 
CAGGCCTTCC TGAAAAACCT TGAGAAGAGA GCAGACCCTC ATTCAGACGC AGCATTTCTC 
TCCAAACTCC TGGACTGCTA CTCCAAAGTG TTCGCCAGGT TTCCTTTGGC AGAACATTGC 
AAAACTGAAA GCTATGCTCG AATGCTGGTT CGATATGCAG AGCTGAAAGG AATTGAAGAT 
CCAGAAGATG CTGCAGATGA TTTCAGTATT GCCAGGTCAC ACTGTAAAGC TTTTGCTTTT 
GTGCACATCG CACATGCTCA GTTTGAGCTC TCACGAGGTA ATTCTAGGAA GAGTGTTTCG 
ATTCTTCAGA AAGCGCTGAG CTCCAACGCT CGACCAATTG AACTGCTGCA AACTGCCATC 
AGGAACCTTA AATCTGGGAA AACTCTTCTG CTTCCTGCTG AGCATCAGGA AAGCTCTGAA 
GCTGAAAATG TGGAAGCACA AAATGGCATG AAGAGAGAAG AAAATCCAGT GAAGGCTCCC 
GAGGATCATC AGAAACCTTT TTCCAAGGAG ACTTCATCAG AATGGAAGAT TCCTGCTCTC 
ATTACCAAAC ACACGTCTCC AGAGGATCGG AAAGCTCCTG TAGAGCCCGT TTCCTCTTCA 
TCTTCCCATC ATGCAGTGCG AACGCCTGCA CCTCTTCGAT TAAACCCCTC ACTCAGCTGT 
CAGACGCCCA ACTACAGACA GCCAAATCCT AACAGTTTTG TGACTCCTGT GGTGAAGCAG 
CGGCCTGTGA TTGTGTCTGT TCCAGCGACG GCTCAGAAAA TGTGTCCGAC TGCGCTGCCC 
TGCACGCCTC AGAGCGGTGT GTCCTACATC CAGCCTCCCA CGCAGACTCC TTCTTCAGCC 
TTCTCAAATG AATCCATAAC AATTAAGGGC AAGCAGTTCT TCATTTTCAA GATGATCGGC 
AGAGGAGGAT CCAGTAAGGT ATATCAGGTC TTTGACCATA AGAAGCACGT GTACGCTGTC 
AAATATGTGA ATCTGGAGGA GGCTGATGCT CAGGCGGTGG AAAGCTACAA GAATGAGATC 
GAGCACTTGA ATCATCTTCA GCAGTACAGC GACCAGATCA TCAAGCTCTA TGACTATGAG 
ATCACCAGCA GCTACATCTA CATGCTGATG GAGTGCGGAC ACCTGGATCT GAACACCTGG 
CTGCGCAACC GCAAGACCGT CAAACCGCTG GACAGGAAGG CTTACTGGAG GAACATGCTG 
GAGGCTGTGC ACACCATCCA CAAACACGGC ATCGTCCACA GTGACTTAAA GCCAGCCAAT 
TTTTTGATAG TGGATGGTTC GCTGAAGCTA ATAGACTTTG GAATCGCCAA CCAGATTCAG 
CCTGACGTGA CCAGCATCAT GAAGGACTCG CAGGTGGGAA CTCTGAACTA CATGCCACCG 
GAGGCCATTA AAGACACGTC CTCCAATGGA AAGCCGGGGT CAAAGATCAG TGCTAAAGGG 
GACGTGTGGT CTCTGGGCTG CATCCTGTAT TGCATGACAT ACGGCAAGAC TCCATTCCAG 
AACATCACCA ATCAGATCAG CAAAATCCAC GCCATTATCG ACCCGTCCCA CGAGATCGAC 
TTCCCTGACA TTCCCGAAAA AGACCTGCTG GATGTGCTGA AGAAATGTTT AGTTCGCAAC 
CCTCGAGAAA GGATTTCCAT TGCAGAGCTG CTCGATCATC CATACCTGCA GCTACAGCCA 
CAACCTGCAC CTGAGCCAGC AGAAACGTCC TCCAGTGATT TTAAGAGGAT CCTCAATGAA 
CTGGTGGCTC TTCAGTCTCC AAACAGTATC GCTAGAGCTG CCAGCAATTT AGCCATGATG 
TGCAACAGCG GGAGGAAACT GGATGTTTCT GAATGTGTGA AAAGCTCCAG TCAGACGCTG 
TGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

ttk ( XM_005157834.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005157891.1 Danio rerio ttk protein kinase (ttk), transcript variant X1, mRNA.

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CloneID	ODa21060
Clone ID Related Accession (Same CDS sequence)	XM_005157834.3 , XM_005157834.4
Accession Version	XM_005157834.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2949bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	dual specificity protein kinase Ttk isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005157834.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGATGAAG AAGAGAGCAC AGAGCGACAG ATGCAGATTG CCATGCTGTG TCAAAAGCTG 
GCCATGATGA AGCAATTGTT CAATGAAGAT GACACGGATT ACATAAACCA GGCCATTAGC 
TCAAACTCCC CGGACACCTG CCGGACATTT CTGAGCAATC TGGAGAAGAA AGGCAATCCT 
CAAGCAGACC CCAGCCTGCT CAGCAAACTT ATGGACAGCT ACACGCGGGT CTTTTCCAGT 
ATGCCATTGG GCAAGTACAG TCAGAACGAG AGCTACGCCA AGATGCTGGT CAGATTTGCA 
GAGTTAAAAG CCATCCAGGA TGTAAATGAT GCACAGACTA GTTTCGATAT AGCGAGGTCC 
CACTGTAAAG ATTTTGCTTT CGTTCACGTC GCTTATGCTC AGTTTGAGCT TTTGCAAGGC 
AACATGAAGA AATGCACTAT GATTCTTCAG AAGGCATTTG AAATGAATGC TAAACCCAGA 
CACGTTCTGG AGGCTGCAGT GCGCAATCTG AAGACCGGCA AACGCCAGCT TCTATCGCAT 
GAAGATAAGG AGAACCTATC AGTATCTGCA CTGGACCACA CACAAGGCTC CAGAAGATCA 
GATGGGACGT GTGAACTGAA GCCCAGCAGC ACTTTTCTCC ACAGTGATCA GAAGTTCAGT 
CCTCAAGAGG AGAACGGGCC CGTGTGGAGA ACAGGATCCC AGCACAGAAG AACAGCCATG 
GCGGAAAGAG TTCCCATGGT GCCTCTGTCT ATCCCTGAAA ATGAGACCAG TGACTCTGAC 
TGTGCTCAGA AAGCAGAAGC TCCTTTTACC CACAGCAGCG GTTTCTCCAG ACAAACAAGC 
GGTTCAAGTG TCCGCTCTGC ATTTTCGTTA TGCTCCTCAA AGAAGGGCAC TCCAGATGGA 
GATTCCTACA GCTTAAACAT AAAGCCACCT GTTATCAGTC CAGATTATCT TCGTGAAGAC 
ATTGAAGAGG GTCACACTAT CACAGCACTT CTAAACAGAG CAGAAAAAAG AGAAACGGCC 
AGAACAGAAG AAACCACTGA CATCAACCAG ATCATCAGCA CAAACTCGAC TGAAGGTTGC 
CAGGCCTTCC TGAAAAACCT TGAGAAGAGA GCAGACCCTC ATTCAGACGC AGCATTTCTC 
TCCAAACTCC TGGACTGCTA CTCCAAAGTG TTCGCCAGGT TTCCTTTGGC AGAACATTGC 
AAAACTGAAA GCTATGCTCG AATGCTGGTT CGATATGCAG AGCTGAAAGG AATTGAAGAT 
CCAGAAGATG CTGCAGATGA TTTCAGTATT GCCAGGTCAC ACTGTAAAGC TTTTGCTTTT 
GTGCACATCG CACATGCTCA GTTTGAGCTC TCACGAGGTA ATTCTAGGAA GAGTGTTTCG 
ATTCTTCAGA AAGCGCTGAG CTCCAACGCT CGACCAATTG AACTGCTGCA AACTGCCATC 
AGGAACCTTA AATCTGGGAA AACTCTTCTG CTTCCTGCTG AGCATCAGGA AAGCTCTGAA 
GCTGAAAATG TGGAAGCACA AAATGGCATG AAGAGAGAAG AAAATCCAGT GAAGGCTCCC 
GAGGATCATC AGAAACCTTT TTCCAAGGAG ACTTCATCAG AATGGAAGAT TCCTGCTCTC 
ATTACCAAAC ACACGTCTCC AGAGGATCGG AAAGCTCCTG TAGAGCCCGT TTCCTCTTCA 
TCTTCCCATC ATGCAGTGCG AACGCCTGCA CCTCTTCGAT TAAACCCCTC ACTCAGCTGT 
CAGACGCCCA ACTACAGACA GCCAAATCCT AACAGTTTTG TGACTCCTGT GGTGAAGCAG 
CGGCCTGTGA TTGTGTCTGT TCCAGCGACG GCTCAGAAAA TGTGTCCGAC TGCGCTGCCC 
TGCACGCCTC AGAGCGGTGT GTCCTACATC CAGCCTCCCA CGCAGACTCC TTCTTCAGCC 
TTCTCAAATG AATCCATAAC AATTAAGGGC AAGCAGTTCT TCATTTTCAA GATGATCGGC 
AGAGGAGGAT CCAGTAAGGT ATATCAGGTC TTTGACCATA AGAAGCACGT GTACGCTGTC 
AAATATGTGA ATCTGGAGGA GGCTGATGCT CAGGCGGTGG AAAGCTACAA GAATGAGATC 
GAGCACTTGA ATCATCTTCA GCAGTACAGC GACCAGATCA TCAAGCTCTA TGACTATGAG 
ATCACCAGCA GCTACATCTA CATGCTGATG GAGTGCGGAC ACCTGGATCT GAACACCTGG 
CTGCGCAACC GCAAGACCGT CAAACCGCTG GACAGGAAGG CTTACTGGAG GAACATGCTG 
GAGGCTGTGC ACACCATCCA CAAACACGGC ATCGTCCACA GTGACTTAAA GCCAGCCAAT 
TTTTTGATAG TGGATGGTTC GCTGAAGCTA ATAGACTTTG GAATCGCCAA CCAGATTCAG 
CCTGACGTGA CCAGCATCAT GAAGGACTCG CAGGTGGGAA CTCTGAACTA CATGCCACCG 
GAGGCCATTA AAGACACGTC CTCCAATGGA AAGCCGGGGT CAAAGATCAG TGCTAAAGGG 
GACGTGTGGT CTCTGGGCTG CATCCTGTAT TGCATGACAT ACGGCAAGAC TCCATTCCAG 
AACATCACCA ATCAGATCAG CAAAATCCAC GCCATTATCG ACCCGTCCCA CGAGATCGAC 
TTCCCTGACA TTCCCGAAAA AGACCTGCTG GATGTGCTGA AGAAATGTTT AGTTCGCAAC 
CCTCGAGAAA GGATTTCCAT TGCAGAGCTG CTCGATCATC CATACCTGCA GCTACAGCCA 
CAACCTGCAC CTGAGCCAGC AGAAACGTCC TCCAGTGATT TTAAGAGGAT CCTCAATGAA 
CTGGTGGCTC TTCAGTCTCC AAACAGTATC GCTAGAGCTG CCAGCAATTT AGCCATGATG 
TGCAACAGCG GGAGGAAACT GGATGTTTCT GAATGTGTGA AAAGCTCCAG TCAGACGCTG 
TGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005157891.1
CDS	121..3069
Translation	MDEEESTERQMQIAMLCQKLAMMKQLFNEDDTDYINQAISSNSPDTCRTFLSNLEKKGNPQADPSLLSKLMDSYTRVFSSMPLGKYSQNESYAKMLVRFAELKAIQDVNDAQTSFDIARSHCKDFAFVHVAYAQFELLQGNMKKCTMILQKAFEMNAKPRHVLEAAVRNLKTGKRQLLSHEDKENLSVSALDHTQGSRRSDGTCELKPSSTFLHSDQKFSPQEENGPVWRTGSQHRRTAMAERVPMVPLSIPENETSDSDCAQKAEAPFTHSSGFSRQTSGSSVRSAFSLCSSKKGTPDGDSYSLNIKPPVISPDYLREDIEEGHTITALLNRAEKRETARTEETTDINQIISTNSTEGCQAFLKNLEKRADPHSDAAFLSKLLDCYSKVFARFPLAEHCKTESYARMLVRYAELKGIEDPEDAADDFSIARSHCKAFAFVHIAHAQFELSRGNSRKSVSILQKALSSNARPIELLQTAIRNLKSGKTLLLPAEHQESSEAENVEAQNGMKREENPVKAPEDHQKPFSKETSSEWKIPALITKHTSPEDRKAPVEPVSSSSSHHAVRTPAPLRLNPSLSCQTPNYRQPNPNSFVTPVVKQRPVIVSVPATAQKMCPTALPCTPQSGVSYIQPPTQTPSSAFSNESITIKGKQFFIFKMIGRGGSSKVYQVFDHKKHVYAVKYVNLEEADAQAVESYKNEIEHLNHLQQYSDQIIKLYDYEITSSYIYMLMECGHLDLNTWLRNRKTVKPLDRKAYWRNMLEAVHTIHKHGIVHSDLKPANFLIVDGSLKLIDFGIANQIQPDVTSIMKDSQVGTLNYMPPEAIKDTSSNGKPGSKISAKGDVWSLGCILYCMTYGKTPFQNITNQISKIHAIIDPSHEIDFPDIPEKDLLDVLKKCLVRNPRERISIAELLDHPYLQLQPQPAPEPAETSSSDFKRILNELVALQSPNSIARAASNLAMMCNSGRKLDVSECVKSSSQTLWK

Target ORF information:

RefSeq Version	XM_005157834.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio ttk protein kinase (ttk), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005157834.4

ORF Insert Sequence:

ATGGATGAAG AAGAGAGCAC AGAGCGACAG ATGCAGATTG CCATGCTGTG TCAAAAGCTG 
GCCATGATGA AGCAATTGTT CAATGAAGAT GACACGGATT ACATAAACCA GGCCATTAGC 
TCAAACTCCC CGGACACCTG CCGGACATTT CTGAGCAATC TGGAGAAGAA AGGCAATCCT 
CAAGCAGACC CCAGCCTGCT CAGCAAACTT ATGGACAGCT ACACGCGGGT CTTTTCCAGT 
ATGCCATTGG GCAAGTACAG TCAGAACGAG AGCTACGCCA AGATGCTGGT CAGATTTGCA 
GAGTTAAAAG CCATCCAGGA TGTAAATGAT GCACAGACTA GTTTCGATAT AGCGAGGTCC 
CACTGTAAAG ATTTTGCTTT CGTTCACGTC GCTTATGCTC AGTTTGAGCT TTTGCAAGGC 
AACATGAAGA AATGCACTAT GATTCTTCAG AAGGCATTTG AAATGAATGC TAAACCCAGA 
CACGTTCTGG AGGCTGCAGT GCGCAATCTG AAGACCGGCA AACGCCAGCT TCTATCGCAT 
GAAGATAAGG AGAACCTATC AGTATCTGCA CTGGACCACA CACAAGGCTC CAGAAGATCA 
GATGGGACGT GTGAACTGAA GCCCAGCAGC ACTTTTCTCC ACAGTGATCA GAAGTTCAGT 
CCTCAAGAGG AGAACGGGCC CGTGTGGAGA ACAGGATCCC AGCACAGAAG AACAGCCATG 
GCGGAAAGAG TTCCCATGGT GCCTCTGTCT ATCCCTGAAA ATGAGACCAG TGACTCTGAC 
TGTGCTCAGA AAGCAGAAGC TCCTTTTACC CACAGCAGCG GTTTCTCCAG ACAAACAAGC 
GGTTCAAGTG TCCGCTCTGC ATTTTCGTTA TGCTCCTCAA AGAAGGGCAC TCCAGATGGA 
GATTCCTACA GCTTAAACAT AAAGCCACCT GTTATCAGTC CAGATTATCT TCGTGAAGAC 
ATTGAAGAGG GTCACACTAT CACAGCACTT CTAAACAGAG CAGAAAAAAG AGAAACGGCC 
AGAACAGAAG AAACCACTGA CATCAACCAG ATCATCAGCA CAAACTCGAC TGAAGGTTGC 
CAGGCCTTCC TGAAAAACCT TGAGAAGAGA GCAGACCCTC ATTCAGACGC AGCATTTCTC 
TCCAAACTCC TGGACTGCTA CTCCAAAGTG TTCGCCAGGT TTCCTTTGGC AGAACATTGC 
AAAACTGAAA GCTATGCTCG AATGCTGGTT CGATATGCAG AGCTGAAAGG AATTGAAGAT 
CCAGAAGATG CTGCAGATGA TTTCAGTATT GCCAGGTCAC ACTGTAAAGC TTTTGCTTTT 
GTGCACATCG CACATGCTCA GTTTGAGCTC TCACGAGGTA ATTCTAGGAA GAGTGTTTCG 
ATTCTTCAGA AAGCGCTGAG CTCCAACGCT CGACCAATTG AACTGCTGCA AACTGCCATC 
AGGAACCTTA AATCTGGGAA AACTCTTCTG CTTCCTGCTG AGCATCAGGA AAGCTCTGAA 
GCTGAAAATG TGGAAGCACA AAATGGCATG AAGAGAGAAG AAAATCCAGT GAAGGCTCCC 
GAGGATCATC AGAAACCTTT TTCCAAGGAG ACTTCATCAG AATGGAAGAT TCCTGCTCTC 
ATTACCAAAC ACACGTCTCC AGAGGATCGG AAAGCTCCTG TAGAGCCCGT TTCCTCTTCA 
TCTTCCCATC ATGCAGTGCG AACGCCTGCA CCTCTTCGAT TAAACCCCTC ACTCAGCTGT 
CAGACGCCCA ACTACAGACA GCCAAATCCT AACAGTTTTG TGACTCCTGT GGTGAAGCAG 
CGGCCTGTGA TTGTGTCTGT TCCAGCGACG GCTCAGAAAA TGTGTCCGAC TGCGCTGCCC 
TGCACGCCTC AGAGCGGTGT GTCCTACATC CAGCCTCCCA CGCAGACTCC TTCTTCAGCC 
TTCTCAAATG AATCCATAAC AATTAAGGGC AAGCAGTTCT TCATTTTCAA GATGATCGGC 
AGAGGAGGAT CCAGTAAGGT ATATCAGGTC TTTGACCATA AGAAGCACGT GTACGCTGTC 
AAATATGTGA ATCTGGAGGA GGCTGATGCT CAGGCGGTGG AAAGCTACAA GAATGAGATC 
GAGCACTTGA ATCATCTTCA GCAGTACAGC GACCAGATCA TCAAGCTCTA TGACTATGAG 
ATCACCAGCA GCTACATCTA CATGCTGATG GAGTGCGGAC ACCTGGATCT GAACACCTGG 
CTGCGCAACC GCAAGACCGT CAAACCGCTG GACAGGAAGG CTTACTGGAG GAACATGCTG 
GAGGCTGTGC ACACCATCCA CAAACACGGC ATCGTCCACA GTGACTTAAA GCCAGCCAAT 
TTTTTGATAG TGGATGGTTC GCTGAAGCTA ATAGACTTTG GAATCGCCAA CCAGATTCAG 
CCTGACGTGA CCAGCATCAT GAAGGACTCG CAGGTGGGAA CTCTGAACTA CATGCCACCG 
GAGGCCATTA AAGACACGTC CTCCAATGGA AAGCCGGGGT CAAAGATCAG TGCTAAAGGG 
GACGTGTGGT CTCTGGGCTG CATCCTGTAT TGCATGACAT ACGGCAAGAC TCCATTCCAG 
AACATCACCA ATCAGATCAG CAAAATCCAC GCCATTATCG ACCCGTCCCA CGAGATCGAC 
TTCCCTGACA TTCCCGAAAA AGACCTGCTG GATGTGCTGA AGAAATGTTT AGTTCGCAAC 
CCTCGAGAAA GGATTTCCAT TGCAGAGCTG CTCGATCATC CATACCTGCA GCTACAGCCA 
CAACCTGCAC CTGAGCCAGC AGAAACGTCC TCCAGTGATT TTAAGAGGAT CCTCAATGAA 
CTGGTGGCTC TTCAGTCTCC AAACAGTATC GCTAGAGCTG CCAGCAATTT AGCCATGATG 
TGCAACAGCG GGAGGAAACT GGATGTTTCT GAATGTGTGA AAAGCTCCAG TCAGACGCTG 
TGGAAATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Cohesin mediates Esco2-dependent transcriptional regulation in a zebrafish regenerating fin model of Roberts Syndrome.
Biology open6(12)1802-1813(2017 Dec)
Banerji R,Skibbens RV,Iovine MK

MicroRNA-133b Negatively Regulates Zebrafish Single Mauthner-Cell Axon Regeneration through Targeting
Frontiers in molecular neuroscience10375(2017)
Huang R,Chen M,Yang L,Wagle M,Guo S,Hu B

Esco2 regulates cx43 expression during skeletal regeneration in the zebrafish fin.
Developmental dynamics : an official publication of the American Association of Anatomists245(1)7-21(2016 Jan)
Banerji R,Eble DM,Iovine MK,Skibbens RV

RFX transcription factors are essential for hearing in mice.
Nature communications68549(2015 Oct)
Elkon R,Milon B,Morrison L,Shah M,Vijayakumar S,Racherla M,Leitch CC,Silipino L,Hadi S,Weiss-Gayet M,Barras E,Schmid CD,Ait-Lounis A,Barnes A,Song Y,Eisenman DJ,Eliyahu E,Frolenkov GI,Strome SE,Durand B,Zaghloul NA,Jones SM,Reith W,Hertzano R

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ttk cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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