znf408 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	znf408
Entrez Gene ID	108004540
Full Name	zinc finger protein 408
General protein information	Preferred Names zinc finger protein 408 Names zinc finger protein 408 zmp:0000000586
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001328275.1	NP_001315204.1	zinc finger protein 408

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

ZNF408 is mutated in familial exudative vitreoretinopathy and is crucial for the development of zebrafish retinal vasculature.
Collin RW, Nikopoulos K, Dona M, Gilissen C, Hoischen A, Boonstra FN, Poulter JA, Kondo H, Berger W, Toomes C, Tahira T, Mohn LR, Blokland EA, Hetterschijt L, Ali M, Groothuismink JM, Duijkers L, Inglehearn CF, Sollfrank L, Strom TM, Uchio E, van Nouhuys CE, Kremer H, Veltman JA, van Wijk E, Cremers FP
Proceedings of the National Academy of Sciences of the United States of America110(24)9856-61(2013 Jun)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

Data indicate that gene knockdown of znf408 showed defects in developing retinal and trunk vasculature.
Title: ZNF408 is mutated in familial exudative vitreoretinopathy and is crucial for the development of zebrafish retinal vasculature.

The following znf408 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the znf408 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
ODa60570	NM_001328275.1 Latest version!	Danio rerio zinc finger protein 408 (znf408), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$391.30 $559.00

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

znf408 ( NM_001328275.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> NP_001315204.1 Danio rerio zinc finger protein 408 (znf408), mRNA.

Price & Availability↑

CloneID	ODa60570
Clone ID Related Accession (Same CDS sequence)	NM_001328275.1
Accession Version	NM_001328275.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2295bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-08-05
Organism	Danio rerio(zebrafish)
Product	zinc finger protein 408
Comment	Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from BX957256.6. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01024746.1, GFIL01024769.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

ATGTCTACAG TCACTGGGAG AAATGAAGGC TCTGGGAACA AGAGAGACTC CATCCACAGT 
GCCCTGAGGT CAGTACCACT AGGGCTCACC GTGGGTCCAT CTCTGGCTGA AGATGAACAG 
CTGGGTCTGT GGTGTGTTGG GCGAGTGCTA AAGAAGGACA CTCTGCTTGG TCTGGAGGAG 
CCTGGAGAAA CAATCAGCAA AGAAAAAGAG GAGGGGTTGC AAAACGCAAG CCAGAGTGTG 
TACAAAAGAG AAATCTCTGA TGAAGTGTGC TGGATGCAGT TTGCCTGCAG TGCTCAGAGT 
GCAGAAGAGA GCAATGTCAG TGTACTTAAA GTGGATGGAA GACTAGGCCT TAAGGTCTGT 
AGGGATATTC AGCCAGGAAC AGAACTGCTC CTTTGGGAAA GTCATCCAGA AGCTGTGACT 
GAAGAAGAAA CGCCTCAAGT AGAAACATGT GAAAACAGAG AACAAGATGA AGCTCTGGAG 
GCCAACACTT CAGATCACTT CTCAGGCTCT CCATATCTTC TTCAAAGAGT CACACAACAG 
GATACAAAAT CTCCAGTTAC AGTAAAAGGT CTTGTGCAGG ACAATACAAT CTGTAATTCC 
AATGTCCTTG AGCAGAATGG GATGGAAGAA AGTGAAGCAG AACATAGTGA TGAACTTCCT 
GCAACAGAAG AGATTGAGAG CATCAATGTG AGTGCATCAC AGGCCCAGAC TTCATCACAA 
TGTCTCACAC CAGAAAGAAG CCTGAGAACC AGCTCTCGCC TCGCCTCTAA ACCCCGAAAA 
GTGCACTCAT CAGCCAAACG CATCTACAAA CGACACGACC CAAAGAACAA GCCAGATCTA 
AGCAGCAAGA AAAAGCTCTT GAATAGCAAA GACGACTTAA TGCAGCCGTT GACAGTTAAA 
GAAGAATCCA GCACAAAGCA AAACGGGCAG GATTTCCCTC CCTTCAATAT CCGAGAGAGG 
AAGTACAAAT GCGAGGAGTG TGATAAAAGC TTCTTTCAAC TGTGCCATCT GAAGAAACAC 
AAATACACTC ATCAAAATCA GAAGCCATAC ACGTGCACAG AGTGTGGCAA GTCCTACAGC 
TCACATGAGA GCTTCCAAGC CCACCTGCTA ATGCATCGTG GCCAGAGGCC CTTTAAGTGC 
GAGCAATGTG ACAAAAGCTA TGGCTTGAAA CGGGACCTCA AGGAGCACCA GGTTCTCCAC 
TCAGGAGAGA AACCGTTTGT CTGTGACATC TGTGGAAAAG CATTCGCTCG TCGGCCCTCG 
CTACGAGTCC ACCGAGAGAT CCATCGGACA AAAGAGCCAG ACTACCAGGC TTTAAAGGTC 
AAGTGTCCAG AGTGCGACAA AGAGCTGGCC AATTCGGGTT CTTTGAGGAA CCACATGCGT 
CTGCACACCG GAGAGCGACC GCACATCTGC CCGCACTGTA ATAAGTGTTT TCGCCAACGT 
GGGAATCTGC AAGGACACAT GCGCATCCAC ACAGGAGAAA AACCTTACAG GTGTGACCAC 
TGCGACCTGA GATTTTCTCA AGTACCCGAA CTGCGTCGGC ATCTGATTTC ACACACGGGT 
GAGGTGTATC TCTGTCCTGT CTGTGGTAAA GCTTTACGGG ACCCTCACAC GCTTCGCGCT 
CATGAACGGC TCCATACGGG AGACAGACCT TACAAATGTG AGCAATGCGG GAAAGGGTAC 
ACGATGGCAA CCAAGCTACG ACGCCACCTA AAGTCCCACC TGGAGGAGAA GCAACACGTT 
TGCCAGGTGT GCGGAGCAAA ATACTCAATG ATGCAGAGTC TACAGCGGCA TCTGCAGCTG 
CACCAGCCAG ACTCTGGACA TGCACTGCCA ACAAGAGGCA GACCCAAAAG GTTAGGCCAG 
AAAGAAGAAG AAGAACCTGG TAGGACTGAG AGCAGCGGTT TGGAGGCAGG ACAGGCTGTG 
GCATGCGCTC AAGCCTCAGA CCACCTCACT ATGGTGACCC ACACAGAAGG GATCATTTTG 
GACTCTGGAA CATACCAACA TGGCACGATT GTTTTGGGAA AGGGAGCGGA GAAGGAGCTC 
GATCGGATTG AGCTCAGTGA GGACATTATT GAGATTTTTA TTTCTGATGA AAACACCAAT 
ATAGTAGTCC AAGAACAGAC TTCTAAAGGT TTAAGCCTTC AAGAACAGGA AGTTAATGCT 
GAATGCACAG TGGAGGCCAG TAGTGACTGT ATTGTACTCC CAGAGCAAGA TGCTAATAGT 
TGTTTGGTCA TAATCCAAGG GCAGGATGGG CTAAGTTCAG TGGCAGAGAC GGTAGAAATT 
GAGACAGTGG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001315204.1
CDS	34..2328
Translation	MSTVTGRNEGSGNKRDSIHSALRSVPLGLTVGPSLAEDEQLGLWCVGRVLKKDTLLGLEEPGETISKEKEEGLQNASQSVYKREISDEVCWMQFACSAQSAEESNVSVLKVDGRLGLKVCRDIQPGTELLLWESHPEAVTEEETPQVETCENREQDEALEANTSDHFSGSPYLLQRVTQQDTKSPVTVKGLVQDNTICNSNVLEQNGMEESEAEHSDELPATEEIESINVSASQAQTSSQCLTPERSLRTSSRLASKPRKVHSSAKRIYKRHDPKNKPDLSSKKKLLNSKDDLMQPLTVKEESSTKQNGQDFPPFNIRERKYKCEECDKSFFQLCHLKKHKYTHQNQKPYTCTECGKSYSSHESFQAHLLMHRGQRPFKCEQCDKSYGLKRDLKEHQVLHSGEKPFVCDICGKAFARRPSLRVHREIHRTKEPDYQALKVKCPECDKELANSGSLRNHMRLHTGERPHICPHCNKCFRQRGNLQGHMRIHTGEKPYRCDHCDLRFSQVPELRRHLISHTGEVYLCPVCGKALRDPHTLRAHERLHTGDRPYKCEQCGKGYTMATKLRRHLKSHLEEKQHVCQVCGAKYSMMQSLQRHLQLHQPDSGHALPTRGRPKRLGQKEEEEPGRTESSGLEAGQAVACAQASDHLTMVTHTEGIILDSGTYQHGTIVLGKGAEKELDRIELSEDIIEIFISDENTNIVVQEQTSKGLSLQEQEVNAECTVEASSDCIVLPEQDANSCLVIIQGQDGLSSVAETVEIETVV

Target ORF information:

RefSeq Version	NM_001328275.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio zinc finger protein 408 (znf408), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001328275.1

ORF Insert Sequence:

ATGTCTACAG TCACTGGGAG AAATGAAGGC TCTGGGAACA AGAGAGACTC CATCCACAGT 
GCCCTGAGGT CAGTACCACT AGGGCTCACC GTGGGTCCAT CTCTGGCTGA AGATGAACAG 
CTGGGTCTGT GGTGTGTTGG GCGAGTGCTA AAGAAGGACA CTCTGCTTGG TCTGGAGGAG 
CCTGGAGAAA CAATCAGCAA AGAAAAAGAG GAGGGGTTGC AAAACGCAAG CCAGAGTGTG 
TACAAAAGAG AAATCTCTGA TGAAGTGTGC TGGATGCAGT TTGCCTGCAG TGCTCAGAGT 
GCAGAAGAGA GCAATGTCAG TGTACTTAAA GTGGATGGAA GACTAGGCCT TAAGGTCTGT 
AGGGATATTC AGCCAGGAAC AGAACTGCTC CTTTGGGAAA GTCATCCAGA AGCTGTGACT 
GAAGAAGAAA CGCCTCAAGT AGAAACATGT GAAAACAGAG AACAAGATGA AGCTCTGGAG 
GCCAACACTT CAGATCACTT CTCAGGCTCT CCATATCTTC TTCAAAGAGT CACACAACAG 
GATACAAAAT CTCCAGTTAC AGTAAAAGGT CTTGTGCAGG ACAATACAAT CTGTAATTCC 
AATGTCCTTG AGCAGAATGG GATGGAAGAA AGTGAAGCAG AACATAGTGA TGAACTTCCT 
GCAACAGAAG AGATTGAGAG CATCAATGTG AGTGCATCAC AGGCCCAGAC TTCATCACAA 
TGTCTCACAC CAGAAAGAAG CCTGAGAACC AGCTCTCGCC TCGCCTCTAA ACCCCGAAAA 
GTGCACTCAT CAGCCAAACG CATCTACAAA CGACACGACC CAAAGAACAA GCCAGATCTA 
AGCAGCAAGA AAAAGCTCTT GAATAGCAAA GACGACTTAA TGCAGCCGTT GACAGTTAAA 
GAAGAATCCA GCACAAAGCA AAACGGGCAG GATTTCCCTC CCTTCAATAT CCGAGAGAGG 
AAGTACAAAT GCGAGGAGTG TGATAAAAGC TTCTTTCAAC TGTGCCATCT GAAGAAACAC 
AAATACACTC ATCAAAATCA GAAGCCATAC ACGTGCACAG AGTGTGGCAA GTCCTACAGC 
TCACATGAGA GCTTCCAAGC CCACCTGCTA ATGCATCGTG GCCAGAGGCC CTTTAAGTGC 
GAGCAATGTG ACAAAAGCTA TGGCTTGAAA CGGGACCTCA AGGAGCACCA GGTTCTCCAC 
TCAGGAGAGA AACCGTTTGT CTGTGACATC TGTGGAAAAG CATTCGCTCG TCGGCCCTCG 
CTACGAGTCC ACCGAGAGAT CCATCGGACA AAAGAGCCAG ACTACCAGGC TTTAAAGGTC 
AAGTGTCCAG AGTGCGACAA AGAGCTGGCC AATTCGGGTT CTTTGAGGAA CCACATGCGT 
CTGCACACCG GAGAGCGACC GCACATCTGC CCGCACTGTA ATAAGTGTTT TCGCCAACGT 
GGGAATCTGC AAGGACACAT GCGCATCCAC ACAGGAGAAA AACCTTACAG GTGTGACCAC 
TGCGACCTGA GATTTTCTCA AGTACCCGAA CTGCGTCGGC ATCTGATTTC ACACACGGGT 
GAGGTGTATC TCTGTCCTGT CTGTGGTAAA GCTTTACGGG ACCCTCACAC GCTTCGCGCT 
CATGAACGGC TCCATACGGG AGACAGACCT TACAAATGTG AGCAATGCGG GAAAGGGTAC 
ACGATGGCAA CCAAGCTACG ACGCCACCTA AAGTCCCACC TGGAGGAGAA GCAACACGTT 
TGCCAGGTGT GCGGAGCAAA ATACTCAATG ATGCAGAGTC TACAGCGGCA TCTGCAGCTG 
CACCAGCCAG ACTCTGGACA TGCACTGCCA ACAAGAGGCA GACCCAAAAG GTTAGGCCAG 
AAAGAAGAAG AAGAACCTGG TAGGACTGAG AGCAGCGGTT TGGAGGCAGG ACAGGCTGTG 
GCATGCGCTC AAGCCTCAGA CCACCTCACT ATGGTGACCC ACACAGAAGG GATCATTTTG 
GACTCTGGAA CATACCAACA TGGCACGATT GTTTTGGGAA AGGGAGCGGA GAAGGAGCTC 
GATCGGATTG AGCTCAGTGA GGACATTATT GAGATTTTTA TTTCTGATGA AAACACCAAT 
ATAGTAGTCC AAGAACAGAC TTCTAAAGGT TTAAGCCTTC AAGAACAGGA AGTTAATGCT 
GAATGCACAG TGGAGGCCAG TAGTGACTGT ATTGTACTCC CAGAGCAAGA TGCTAATAGT 
TGTTTGGTCA TAATCCAAGG GCAGGATGGG CTAAGTTCAG TGGCAGAGAC GGTAGAAATT 
GAGACAGTGG TATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

ZNF408 is mutated in familial exudative vitreoretinopathy and is crucial for the development of zebrafish retinal vasculature.
Proceedings of the National Academy of Sciences of the United States of America110(24)9856-61(2013 Jun)
Collin RW,Nikopoulos K,Dona M,Gilissen C,Hoischen A,Boonstra FN,Poulter JA,Kondo H,Berger W,Toomes C,Tahira T,Mohn LR,Blokland EA,Hetterschijt L,Ali M,Groothuismink JM,Duijkers L,Inglehearn CF,Sollfrank L,Strom TM,Uchio E,van Nouhuys CE,Kremer H,Veltman JA,van Wijk E,Cremers FP

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znf408 cDNA ORF clone, Danio rerio(zebrafish)

Gene

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gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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