HECTD4 cDNA ORF clone, Danio rerio(Zebrafish)

The following HECTD4 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the HECTD4 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

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ODa51539 XM_009303668.3
Latest version!
Danio rerio HECT domain E3 ubiquitin protein ligase 4 (hectd4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa51540 XM_009303669.3
Latest version!
Danio rerio HECT domain E3 ubiquitin protein ligase 4 (hectd4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
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ODa51540 XM_009303669.2 Danio rerio HECT domain E3 ubiquitin protein ligase 4 (hectd4), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
ODa51539 XM_009303668.2 Danio rerio HECT domain E3 ubiquitin protein ligase 4 (hectd4), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector TBD Quote Price
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa51539
    Clone ID Related Accession (Same CDS sequence) XM_009303668.3 , XM_009303668.2
    Accession Version XM_009303668.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13260bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303668.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1981
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    12781
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    13081
    13141
    13201
    ATGGGCTCAG GTGCTGTGGA TTCTTCCCAG TGGCTGTCAG TGAAGGAAGA GACTATTTTC 
    CTTCACGATG GACTAATTCG GGTCACGGAC CTGGCTGAGC TGCCCAGCGA AATCGGCGTG
    TCTGAACAAG CTGAATCCGA GCAGGAGATC TTGACGTTTG AGACCAAGAA TCCAGTGGAG
    CTAGCCGAGC GTCTCCGAGC CGTCTGTGGA AACCAGAGCA ACGCTTATGC ACGCCTTTTG
    GAGTATCGTC TGAATGCACT GCGTGGCCTG TGGGGGGCGC AGCGTCAGCT GGCCCTGGAG
    GAGCAGCAGG ATCATGACGG GGTTGTGTCA GCCAGCGGCG GAGGGTCCGA CGAGGAGACA
    CTGGCCCTAC TCAAACGACA GGGTTTACTG CAACCCCAAC ACCACCACCA TCCACACCAC
    CCTCCGATGG CCAGCGGCTC AGAGCAGGCA CCATTCACCT CCCGTGTGGG CCTGCTGTTG
    GTTTTCCCAC TGCTGCAGTC GCAAACTCGG CACGACCCTG CGCTGTGCGG CGTCACAGCG
    GAGGTGTTGC TCGCATGTCT GCGGGACTGC CAGCCACTCA GCCTGGGGAA GGAGCCCGCC
    GACTGTCTTA ATGGCCTGGA GAGGTTGCTG TGCTCCTGGT TGGAGGAGAC GGAGGGTTCG
    GCACCAATGA GGCCACTGCT CACCCAACAG CAGAGACAGA ACGCAGCTGC GGCGCTAGTG
    GCTCTGGCAT GTGCAAGGGG CTCCCTCAAA ACCTTCATCC ACACGGTCCA CCTGCTGCAG
    AAACAAACAG ACCTGGGTCA GCTTCCTGTG GCTGATGTTC TCTATCGACT GCTGCTTCTT
    GAAGGAGGTC CAGGTTCGCC CTCATGTCTG CTGGGGGGGA AACATATTGT GTCGTGGGGC
    TTTGAGGACA TGCTGCCCAC ACCAGAGAGC AGCGCCGCAG GAGCCGACAG TAAAGACACA
    GATCTGGGCC GCAGCCTGGC CACAGATGGA TTCTATCTCT ACACCACGAG CTCTTTTGGG
    AAGGGTTTGA GCAAGCTGGG CTCCGGGCAG CACGGGACGC TGAGGGGTTT TGTGTACTGT
    CGTAATGAGG AGATGGAGGC GGGCTGGGTG GCTCACAGCA GTGGATTTCT GCTCCACCGT
    CCTGCATCAT TTGACACTAA ACCTCATCAG CTGTGCCACG TCATCGACCC GTTCACACTG
    CAGGTTTCTC AGGTGTTAGC GATGCCCGTT CATCATTTCC CAGTGGGCAG CTGCCTGACC
    ACGCTGCGTC TGTGTTCTGA CGGCACATAC TTGTATTGGG TTTGGTCTCC GGTCAGCATC
    AACGAGAAAA CGCAGAAAGG CCACTCTGTC TTCATGGACG TCTTCCAGTT AACGAGTGAG
    TCCGGTGTGT GTGTGGCCAG TCCTGTACAA GACCGGGTGA TCCTGTTGCG AAAGGAAGGC
    GAGTCGTCCA AGTGCTTGAA TGAGTTGCTC CTCAGCCGAA TGTCGCGCTT CCGAGCCTCG
    CACTCGTCCA CACTGGCGGC TCTCACCGGC TCCGCCATCA CCAACCCTGT CAAAGAGGAG
    CAATCGGTGG TCAACACAAG CTGTGGTCTG TCCATCAAAA CACTGAGGAA GACACCCATG
    TATGTGTGCG GGACGTCCCT GGTGATGCTG GTGCCGACTC CAGGTGTTGC TGGGAGCTCC
    GCCACGCGCT CGCTGTTCGG GGGATCCAGT GGCCTTTCTT CTCTCAAGAT CTTGGCCTCT
    AGTTTGGTGT TTAACCTGGC GGACGGTCAG TTCAGCAATC GCGTGGATCT GGTCGACGCT
    CCGGGAAGTT CTCTGGGTCG TGGAGCTCAG GTTGCAGGAC TCGGCGCCTG TTATGATGCA
    CTGAACAACT TAATCTGGAC GTGTTCGAGC GACTACATGG ACCAGTGGTG CAACCCTGGA
    AATCAGGCCT TCCATCATGT GTGCCAGCGA CTCGGCGTCA GTCACGCCAT CAGCCAGACC
    AAAGAAGAGA TGATCGACAC ATCCAGCGTG ATCAGCCAGC TGCTCCATCA TGTGGGAGCC
    ATGTGTATCC ATCAGCTGAA CCTGCTGGCC GCCAGCAGTA GTTTACACTT GGGCACCTTC
    ATAGGGAAGC AGCCAATGGA GGCCTGTCAC TTCAGCTCCA TCTGTGACGT GATGGAGAAA
    GCCATGGTCA ACGGAGACAC CCGCATCATC CGCTGCATCC TGGTCGTTTT TCAGGTGGTT
    TTCCAGTTTT TTAATCCAAA CTCAGAGCAG AACCGGGAGT GTGTGAAGCG CTCAGGACTG
    CTGCTGTGGC AGTTACTCAT GGCTCCACCT GATCAGATCC AGCCGGAAAT ACAGAAAGAA
    GTTTGTCTCG CCATCAGCTC AGGTTTAAAC ACCCTGTATC AGAGCGAATC TGAGTTGAAC
    AACCTGCTGA AGTTTGTTTT CACAGAGGGG GAGAAGAACA GTGGTTTGTC TCAGTTACGA
    GACGTGATTT TAAACAATCT GGCTAACCAG TTACAGAAGA ACCGCTTTGG TAGTGAAGAT
    GATGATCACT ACAGGCTGAA GGATGAGCTG CTGCAGTGCA TCTTGAAGAC GGTGGTGCGC
    GAGTCGTGTC TCCTCATCAC CAAATGTCAA ACCGTTTCCA AAGAAGACCT TCAGAAGCTG
    CTCTCTACTG TACCTGTGGT GTCTCCCAGC TTGCGTTATT TAATGGCTGT GCAGAATCAT
    CTGCTCAGCA ACACCATCCT GCTGCACCCG GAGGACATGG AGGACAGCGA CAGCTCCCTG
    CAGGGGGAAA CAATGAAGGT ACAGGAGCTG CAGACGAGCA TCTTGTCTCT TGCGTTGAAG
    ATTCTGGTTG GATGTGATGA AGTTCTGGAG ACGCTTCAGC AGGTGACCAG CTCTCTCATC
    AACAGCAGCA TCAGCGACCG AGAGACCAGG CTCAGAGGTC TTGAACAGGT GACCAAGGCC
    ACCATGTTGG GTCATTTGCT GCCGGTTCTG CTGACGTCAC TGATGCACCC GAACCTCCAA
    ACACTCACGC TAGCAGATGC GCTCATGCCA CAGCTAGTCC AGCTCGTCCT CTACACTAGC
    CAGACCGCTT TGCTGCTAAA GACACATTCT CCATTATTTG TTGAGGATTC CCCAATAAAT
    CAAGGCTCCA GCCAGCCCTC ATTAAATGAC AGTTTTCAGA TTCTGGAGGA GAGAGAGGAG
    CCTGGCTTTC TGACGGGGCT GAAGATCCCC GCCCCGTGGG CTGCTGGGAA AACGGTGGAA
    ACTGTTCACC CGGTGCGGGA TAACTATAAA TTTAAAGACA CAGTGCACAT TCCTGGAGCT
    CGCTGTCTGT ACCTGCGCTT CGACCCGCGC TGCTCCTCAC AGTACGACTA CGACAAGCTT
    GTGATTTACG CCGGGCCGAA CACAAACAGT CGTAAAGTAA GCGAGTATGG AGGAAACACC
    ATGGGTTATG GCAGCCGCAG TGTATTGGGA ACCGGATGGC CAAAAGATCT GGTCAAGGTG
    GAAGGAGATA CGGTGACGTT CTCTTTTGAG ATGAGAAGTG GACGCGAGCA CAACACTCCA
    GATAAAGCCA TGTGGGGTTT CTCGTGCACC GTCAGAGCGC AGGAGTCGTC TGAGGATGTG
    TCTGGCGGTC TGCCGTTTCT GGCTGATCTG GCTCTGGGTT TATCAGTGTT AGCCTGCGCT
    ATGCTACGAA TCCTCTATAA TGGGCCTGAA ATCACACGAG AGGAAGAAGC TTGTCACGAC
    CTTCTCTGCT CCAAATTACT GCAGAGGTGT CAGTGGCAGG TGGAGGCGAA CGGAGCCATC
    TCTCCTGCAC TCACGCCATC TCCGTCTCCT CTTCCTCTGA CTATCGATGA GGAGCGCGAG
    TTCACATATC CATCAGATAT CCTCGCTCCG CCGCCGGGGC CCATCCCGAG CGCCTGCTGT
    GAGCTGCCCC GCATTCACTT ACCGCAGGGT ATCATGGGCC GCCTGAGGGA GCTGTCTGGA
    CGTGCTCGGC CACAGTTTCG CCCCAGCATC AAAGAGGTGA TCCGTCCAGA CGTGATGGAG
    GAAGTGATTG TGTCGTGTGT GATTAAACAC TTGGGTCTGG TGGACGCGCT GCAGTCGCTG
    GTCAACTTCC AGTACAGAGA ATCTCACAGA GAAGAACACA ACCTGCTCTG CAAGATCATG
    GCTGAGACCT TCAAGAAGAT CAACGCCATG GAGAGACAGT TACAGAGCGT GGCTGAGCTG
    GAGCAGAAGT GGCAGAATGA AGTAGATGAA GCTCAGCAGG GAAAACTGGA GAACAACACT
    CCTTTCTTCC ACGATTATCA CTTCTTTGAG AATAAAATGA AGGAGCTGGA GCTTCTCTGT
    TCGCTAAAGG AGGTTCCTCT GGACTGCACT GATCCAGAAA ACGCTGTGAT GGCTTTGAGA
    GAGAAGTTTT TTCATGAGGT GAACTCCACG CTGCAGCAGC AGAGAAGTGT TGTTCCTCTG
    GCTAAAACCA AAGCGCTTGT GAAGAGTTTG ATCAACCGCT CGGAGCTGCT GCTTCATGTC
    ACCATCGCTC CGCAGTGCAA GAGTCTGTCA TCAACACCCA CAAGCACACC GGCCTGTAAG
    TCGGTGTCAG ACGCTAAATC GATGGCTCCC GTGGTGAAGC AGCCGGTGTT CCTGCGCAGC
    ATGTCTGCTC CGTCAGATCT GGAAATGATC GCCAGCAGTG ATGTGGAGTT CACACACGGA
    GCGCAAAGGA GGCGGCAGCA CCCCTCCAGC CACCGGAGCA GCTCATTTAC ACTCCTGCAG
    TCATTGGTGA TTGAAGACAG TAAAGATAAA CCCACGTACA GTGTGCTGCT GGGACAACTG
    TTCGCCTTTA TAGGAACCAC TCCTGACCAA ACCGTGTCGA GCAGCAGTTT TCTGTCCGCA
    GCTCAGACGC GCTGGAGAAG AGGACGAACA CGCAAACAGG CTCTGGTTCA CATGCGGGAG
    CTGCTGACCG CTGCCGTCCG CGTCGGTGGA GTCACTCATC TGGTCGGACC CGTCACTATG
    GTCCTGCAGG GGGGACCCAG GGTGGAGGAG CTCACCTGTG GTGGGATGAT GGAGCAGGTG
    CAGGAGGCGT TTGGAGAGAC CATGACGTCA GTGGTGTCTC TGTGTGCCCG ATACCCCATC
    GCCTGTGCCA ACAGCATCGG CCTGCTCTGC ACCATCCCCT ACACCAGGAG TGAGGAGCAG
    TGTCTGGTGC GCAGCGGTCT GGTTCAGCTC ATGGATCGCC TCTGCAGTCT GAGCAGTCAG
    AGAGACTGCA GCTCCAACGA GAAGCAAACC AAAAAGCAGA AAGTGGCCAC CATGGCCTGG
    GCTGCGTTTC AGGTGCTGGC CAACCGCTGC ATTGAGTGGG AGAAAGTCGA AGGAGGCTCC
    ACAGACGCAG TGCATTCAGG GTTGGCTCGA CAGGTGTCCA GCCTTCTGAC CAATCATCTG
    GCTCGAGCGA CAGACTGCTG TGGAAACCAG GCGGCAGGAA ATGATGCGCT GCAGGATGTG
    CTCAGTTTAC TCAACGATCT GTCCAGGAGT CACATAGGCA AGACCATCCT GAGCCAGCCT
    GCCTGTGTGT CCAAGCTGCT GTCTCTGCTT CTGGACCAGC GACCGTCGCC CAAACTGGTG
    CTGATCATCC TGCAGCTGTG CCGCGCCGCG CTGCCCCTCA TGAGTGTGGA GGATTGTGGA
    AATGTAGCGC TGCCGTCCTG GAGCTACTCC ACAAACGCAC TGGACGCCGA GCAGCAGGAC
    GCCAGCGACC CTGCCTTCCG CATCGCATCT CTGCTGCTCG CCAAACTGGC CGACTATGTG
    GTGCCAGGCT GTCAGACGTT GCTCTCTCCC AGCGCCTCTG AGCCGGACAC CAGTCTGAGC
    CGAGCCTGTC CCAAGAGCAG CTCTAAGACC GACAGAGATG CCAGCGAGGA GGGCGAAGCG
    GTGGACGGAA AACTCTCCAT ATTCATCCAT AAGAGAGAAG ACCAGTCGTC GCACGAGGTG
    CTGCAGCCTC TGCTCAGCAG CTCAGAGGGT CGACCGTTTC GTCTCGGCAC TGGAGCCAAC
    ATGGAGAAGG TGGTGAAGAT GGATCGAGAC ATGACCAAGA GCGGCTGCTG TGAGGTCATC
    ACTGAAGAGG CGTCCGCTGC TCTCAGGAAA GCCAATAAAT GGGCTCAGTC GGGGCTCATT
    GTGAGTGTGG GTCCGCCCAT CGAAAGCAGC GCTCAGGAGA ACTCAGGAGC GACCTCTACA
    GGAGACAAGA AGAAAAGCGC TCAGTCCACG GTGTGTCGAG AGAGGAACGC TGAGCTCGCC
    CGGTCTGACC CGGTGCGTCC GTTCATCAGT GGTCACGTAG CCAACAGTAT GGCAGCAGAG
    GTCATCGCTC TGCTGCACAG CCTCCTGACG GCCCCCGAGT CCAACACTGC TCAGATCTGG
    ACAAGCACTG CAGAAAAGGT GTTGTCCAGA GCTCTGATGT TCATCCCTCA GCTGGGGAAG
    TTTGCTGAGA GTATTCTGGA GAGCGGAAAC AGCAGCGGCA GGAAGTTGTC CAAGCTGCAG
    GCCATTGGTC GACAGGCGGT GGCGGCTCTC TGTGCGCTCG GCGGATTCAA GGAGACCATC
    AAGATTGGAT CTGAAGTGCA GGTGGTTGGT AAAGGTGTGT TGGACAGTGT CGGTGTGGTT
    ATATCCATTA ATGAGCAGGA GGGCATCGCC ACCGTCAAGT TTCCATCCTG CGAGTATCGG
    CGCTCCAGCA AAGCATCTGA TATCCTGACG GTGCCCATAT CACGCCTGTG CACGCCGCGC
    TCAGAGGCTC TGCCGCTGCA CAAGCTTTCC ATCACAGAGA AGGTTGTTCA GGCCGTTCAG
    TCCATGTTGC TGCCGCAGGA GGGCAGTCTG TCCATACACA CGTCTCTGCC GGCGTCTGGA
    GACGGCTCCA GTCCTGTGAT GGCAGTGGTG CGTCTGCTGG CCGAGATCAG AACCAGAGCG
    TGTCTGGTGA TGGCGCAGCT GCTGGAGGAC AGCTCTTTCT GTGAGGAGTT TATCCAGCAG
    TGTCCTGCAG CCGTGGAGGT GCTCAACCTC ATCGCTCAAG AGTGCAGTCC TGGAGAGCGT
    TTGGTGATGG TGGAGGCTCA GTGTGAGCGT CTGCGCATGC TTTATCGAGA CTGTGCTCGT
    CCTCCACCAC CGCCTGTGCA AACTGACCAA CGGCAGCCGA AGGAGATCAC GTGGTGCCCG
    TCTCGAGTCT TCCCTCCGGT TCGGGCCTGT ATGTTTTCCT CGCACTTGAC GTCGGTGACG
    TTTCTGGCTG ATCCGAGTGC AGGCGGAGGC CTTCCCAGAG GAACCTTCAT CTACGCCACT
    TCACCCGTGC CCATTCAGGC TCCGTCTTTT TACTGGGAGA TCGAGATCGT TTCGTTCGGA
    GACTCGGAGG ACGACAGCGG TCCAATAGTG TCCTTTGGAT TTTCCCCGGA GGCGGAGAAG
    AGAGACGGAG CCTGGACCAA TCCCGTCGGG ACCTGCCTGT TTCACAATAA TGGCCGGGCG
    GTTCACTACA ACGGCTCCAG TTTACTGCAG TGGAAAAGTG TGAGGCTCGA CGTGGCTCTG
    CTTCCTGGTG ATGTAGCCGG CATCGGCTGG GAGCGTCTGG AAGGCACTCC TCCTCCACCC
    GGTCAGCCTC CCAAAGGAAG AGTGTACTTC ACCTACTGCG GTCAGCGACT CGCTCCCTAC
    CTAGAGGATG TGGCTGGAGG CATGTGGCCC GTCGTTCACA TCCAGAAGAA GAATACGAAG
    ATTCACGCCA ACTTCGGCTG CCGGTTGTTT GCGTACGCTG AAGGCCAGGC TCACAGAAAC
    GCTGCTGATC TCTGCATGGA TCTGTCAGAG GAGATCAGTG CTAACTTCGA GGCTCTGCCC
    TTCGCCATGG CCTCCGACAG CGATAATGAT GCGGGCACCA GTGTGGCGTC TGACTCCGGC
    TCCCACGGGC CTCCTTGTCG CATCGCTGCC GTCGTCACAG CACAACAGCA ATACAACAGC
    GATGCTTCTT GCCACTACAA GGTAGAGCTG AGCTATGAGA ATCTGGTGGT GTCGGGACCG
    AACGCTCATC CGCCACCCAT CGCTGATGAC GAGAGCGATG ATGAGGATGA TGAAGACATT
    CCCCGGGAGG ATCATTACGC CCTGCTAGTG AAAGCGTGGG AAACTAAAGT GTTTCCCACC
    ATTCGCCGGC GGTTCAGGAA CGAGGCGGAG AGGAAATCTG GCCTGGATCA GATCAAGGGA
    GCGCTGCAGC TCGGTATGGT GGACATCGCT CGTCAGACGG TTGAGTTTCT GTATGAAGAG
    AATGGCGGGA TCCCTCGAGA CCTCTACCTT CCAACCATCG AAGACATTAA GGATGAAGCA
    AACAAGTTCA CCATCGACAA AGTCCGCAAA GGCTTGACGG TGGGCATCCG CTGTCCAGAG
    GGAAACAACA CCTGCAGCAG CACAGGAGGA ACCGCTCTAC CAAAGTTTGC CATCCGCGGC
    ATGTTGAAGA CCTTCAGCTT ACATGGAGTT GTGCTCGATG TGGATTCGGG TAATGAGCTG
    GTGCAGGTGG AGACGTATCT GCGCAGTGAG GGTGTGTTGG TGCGCTACTG GTACCCCATA
    GAGATGCTGG AGAGACCGCC GGCAGGATCC CGCAGGGCTG CAGCCAATGG CCTGGTGTCT
    TTGGACAGCA GCAACATCCA GATTCATCGG GAGCTGCTGC GCTGTGAAAG TGCTCTGGCG
    CGTCTGTACT GCCGCATGGC GCTGCTCAAC ATCTTTGCGC CCAAGAGTCC TCACACATTC
    ACGCGCCTCT TCCACATCCC TGCAGTGCGT GACATCACAC TCGAACACCT GCAGCTCCTG
    TCCAATCAGC TGCTGGCTCC GCCTCTGCCA GACGGCCGGA TCAGCTGTAA CTCTGTGCTG
    CTGGCTCAGT CGGTCCTACA TGAACTGCAG GGTCAGAGCT GCTCTCCCAC TGAGCTCTTC
    TATCAGGGAA ACGCTCAGAG TGTCCGCGAG TGGCTGAGCG TGGCCATCAG CAGAGCTCTG
    CATCAGGGGG AGGACAGTCT GCTGGAGCTC ACCAAACAGA TCTGCTGCTT CCTGCAGAGT
    GCGCCTGAGC AGTTCACGCT GGAGGAGATT CCCATCACCG AGTCAAAGGT CAGCATGGAC
    GTCAACTTCC CCGGCGCTGC GTTTGTGCTC GTCTCCTGCA AAGAGAGTCA ACTCAGCTGC
    CGCAAAGATT CCTCTCTGTA CAAAGCTCCA TGGGCTCGAG TGATGGTGTA TGGGCTCGGT
    CATAAAGTGC GGCGTAACGG CCAGCTGAAC CTGATGGAGG CCGTGTGTTT CCCGCTGGAC
    GCCTCTCCGT CCAACACGGG CCTCACTCCT CCACCCACAG CCAACCAGTA TCCCAGCATC
    ATCATCCCCA CCGACAGAGT GCACGTCAAA CTGGGTGTGT CTCCCCCTCC TGGAGCAGTG
    CTGGTGTTGC ACTCGTTGCC TCTTGAGTTT CCATTGGCGA TGGCCTTCGC TGAACAGCTG
    CTAACCTACA GTGTAGGAGA GACCGTTGAG CGCTCTGAGG ATGAGCTGGA CACGGTGCCC
    ACCTCTGTGC TCATGCAGGT CGTGGAGCTG CTAGGTGGCC TTTTGTGGAC CACAGATCTT
    GCTCCGAGCA TCAAGGAGCT GACCTTCCAC CTGCTTGCAG AGCTCCTCCG CAAGATCCAC
    GCTTTTGAGC GCCGCAAATC CCCTTCGGGT CTCTCTAGCC ACATCACGCT GCTTCTCAAC
    CCCTGCCTAA CAGTCCTGAT GGTTCTTCAA ACCGAACTAC GAAAGCTTTA CGACCACGAG
    ACCCAAAGCT GGGGGTTGGT TAGCGGAGGA TCCTGCACTT CCGCAACAGG CGGCAGTGGA
    GCGCTTGCAC TTTCTGCAGA GCGGAGCCGC TTCTCTACAT ACTTCCACGC ACTGATGGAG
    GTTTGCCTGG CAGTGGCGGA AGTCACTTTA CCGCTGAGTT CTGGTTCTGG GACGTCAGTA
    ATCGGCGTCC CTTCATCAAC AAGCACAGCC AACTGCAGTG ATTCCTCATC CTCTTCTTCG
    TCCTCTCCAG GCCAGACGCC GCAAAGTCCG AGTTTGCTTT CAAAGCGCAA AAAGGTGAAG
    CTAAAACGTG AGCGCGCTGC AGTCGGCTCA GCTTCCGCCG TCTCAGGGAA AAGTGGCTCG
    AGATCATCAG AATGCGACAA TGCTCTATTA AACATGAAGC CTGAAGACAT GTTGTGGTTT
    CACCGGGGGT TGACCTTGCT TTTGATTTTA CGGCATCTTA CTAATAAGGA CCCGCAAGGT
    CTTGGCGTCA CCGGCGACGC TGTGGCAGAC GCTTGCCAAG CTTTGGTTGG CTCGACAGCT
    CACAGTCGCT TGCTGGTGCT CTCAGGAATC CCGACTCACC TGGAGGAGTC TGTGGTGCGC
    AATGCAATAC GAAGAGCATG CAATGCTCAT GGCGGGCTCT TCAGGGATGA GATCTTCATT
    CCAGTTCAGG AAGAAGACCC TAAAAAAGCC AAAGCGGAAA CGACATGTGT TCCTCAAGAT
    GTAAAGAGAG AATGCACTGC AGGAAGCCCC AACAGCAACA GTGCGGCAAT GAGTGTCTCC
    AGCGCTTTAC AGGCAGCCTT CAGAGCCTCC GGAGAGCCCT TGTCCAATCA GGAGATGGTA
    AACAGTCCTT CCCAGCAACA AGCACCTCTG GATCCACTCA CAGTGTCCAG TCAGGAGAGT
    CTGGACGTGT CTCTGTGTAG CACAGAAAGT CAAGGGAGTC TCGGCAGCCT CGGGGAGCAG
    GATAGTGCTG CCACGTCAGT CTCAGATGGG AGCTCCATGT ACACGGTGGC CTCACCTGAT
    GTTCATGCCA GTGTGACGCG TCCCATAAAA GGCTATGCTG TCATTGAGGT AAGGGCACGA
    GCTCAGGTGG AGAAGATTCG GGCCAGTCTT TTCAACAGCA GCGATCTGAT TGGCCTCTCC
    AATCTGGAAA GGGAGGAGGA GCTAATGGAG ATGACCAATG AGGAGATCCT CACCGCCTCC
    AGTGTCAATC AGAGTTTATT TGACACACAG GGCAGCTCTG CTCTGGAGGA GTACTTCCTG
    GATAAATCCA TCAGAGGAGA CAAGCTGGTT CCTGGAGCTC GTGACGTGCT GACAGACATC
    TTTAAGAGCT GCATATACGC AGAGCAGGCG CTGAGCCCCG TTCCCGTGAA ACCTGTCAAA
    GTCTCAGACA TTTACCTCAG CAAAGAGCAG ATCAACTCTC AGTCTCCCGG AAACCTGCTA
    CACGTGTTTT TCACACACGT TCGGCCTCCC AAAAAAGTGC TCGATGACCA GCTCACACAG
    ATTCTGCGGA AATACGGCAC AGTAAAGCCC AATAAAAACA AGCACAGCAA AGCGGGCAAA
    GAGCAGCACC AAGGCAAAGT GGTGAGCACA AAAAGACCCA TCACCAAACC TCCGGCCAAA
    GAGAAGTCCA TGCTGAACAG CGTGCGAACT GTACTGAGTG AGAAAAAAAC CGTCCTCAAG
    CCAAAATCTC CAGAGAAGAC CAAACCCGAT GAAAAAGACC CCGAAAAATC TCCTGCCAAA
    AAACCCGAAG TGCCAGAGGA GAAGTTTTTG ACGCTGGAGG GTTTCCACAA GTTTACTCTG
    GACAGAGCCA AGCAGGATAT CCGCAGTGTT TGGCGGGCGA TCCTGGCCTG TGGATATGAC
    CTGCACTTTG ACAGATGCAC ATGCATTGAT TCCCGACATG CTCAGAAAGC CAGCAGAAAA
    TGGAGCTTCG AGATGGACTT TGCGTTGGTG CAGTTTGTGA ACCGTCTCTG TCGTCACCTG
    GCCATCACTC CTGCCCGCCT GCATCCACAT GAGGTTTACC TGGACGCCAG CAAAGATGGC
    GCGGATCCTG AGGTCGCGTG TCTGTTAGGT GTCCCAGTGG AGAGTTTGCG TCTGCGCTTT
    GCTCTGCTGC AGTCCCTCAA CAGCACGCTG GAGAGCTTCT TCCTGCCTTT AGTGGAGCTG
    AGACTCACAC AGACCTACCA GAACAGCATC GCCACACTTC TGTGCGAGGC CAAAGGCCTG
    ATATTTTACG ACACTAAGGT GACGGTGATG AACAGGGTGT TGAACGCTAC CGTGCAGCGG
    ACGGCAGATC ACGCAGCGCC TGAAATCACT CTCGACCCGC TGGAGATTGT TGGAGGAGAG
    ATGCGCGCTG CGGAGAACAC TTATTTCTGC CAGGCAGCGC GGCAGCTGTC GTGTGTGCCA
    TCGTCTCAGC TGTGTGTGCG CATCGCCAGC GGAGGAGACC CCACATACGC CTTCAACATC
    CGCTTCACCG GAGAGGAGGT CCACGGCACC AGTGGCTCTT TCAGGCATTT CCTATGGCAG
    GTGTGTAAGG AGCTCCAGAG TTCAGCTCTC TCTCTTCTTC TGCCCTGCCC GAGCGCCGCC
    GCTAACCGCA ATAAGGGGAA GTACATCCTG ACTCCGTGTC CCATCACATA CGCTGAGGAG
    CAGCTGCTGA ACTTCTTCGG GCAGCTTCTG GGCATCGCCA TCCGGGCCGA TGTGCCGCTG
    CCTCTGGACC TGCTGGGATC CTTCTGGAAG GGTCTGGTCG GAGAGGCTCT CGATCCCGAA
    GCTGACCTGC GGGAAGCAGA TCTGCTCACG TACAGCTACA TCAAGAAGTT TGAGAGTTTG
    TGTGATGAAG CGGAGCTGGA AGCGCTGTGT GCTGAAGTGT CGTCTCAGAG TCCCCTGGGG
    GAGAGTCCAG ACTCCCCCAG CCGGCCCTGC TGCACCTTCA CATACATCAC TATGACTGGA
    GAGGAAGTGG AGCTGTGCCA GGGAGGACGT GACATCGCTG TCAGCTGGGA GAATAAGGAT
    GTGTATGTGC GAGCGGTGCG TGAGCTGCGT CTGCGGGAGC TGCAGAATGT GGAGTGTATG
    TCTGCGGTGC GCGCCGGTCT GGGCTCCATC ATCCCACTGC AGCTGCTGAC TTTACTGAGT
    CCCGCAGAGG TGGAGCTGCG CACCTGCGGT CTGCCGGACA TCAACCTGGA GTTCCTGAAG
    GCGCACACCA TGTATCAGGT GGGTCTGATG GAGACGGATC AGCATATCGA GTTCTTCTGG
    GCGGCGCTGG AGATGTTCAC GCAGGAGGAG CTCAGCAAGT TTATTAAGTT CGCCTGCAAC
    CAGGAGAGGA TTCCCTTCAC CTGCCCGTGC AAAGATGGGG GTCCGGACAC CGCACATGTG
    CCCCCCTATC CCATGAAGAT CGCCCCGCCG GACGGCACCG TGGGCTCTCC AGATCAGCGC
    TATATCCGCG TGGAGACGTG TATGTTCATG GTCAAGCTGC CGCAGTATTC CTCCCTGGAT
    GTGATGCTGG AGAAACTGCG ATACGCTATT CACTATCGAG AAGATCCTCT GAGCGGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301943.1
    CDS431..13690
    Translation

    Target ORF information:

    RefSeq Version XM_009303668.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT domain E3 ubiquitin protein ligase 4 (hectd4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303668.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGCTCAG GTGCTGTGGA TTCTTCCCAG TGGCTGTCAG TGAAGGAAGA GACTATTTTC 
    CTTCACGATG GACTAATTCG GGTCACGGAC CTGGCTGAGC TGCCCAGCGA AATCGGCGTG
    TCTGAACAAG CTGAATCCGA GCAGGAGATC TTGACGTTTG AGACCAAGAA TCCAGTGGAG
    CTAGCCGAGC GTCTCCGAGC CGTCTGTGGA AACCAGAGCA ACGCTTATGC ACGCCTTTTG
    GAGTATCGTC TGAATGCACT GCGTGGCCTG TGGGGGGCGC AGCGTCAGCT GGCCCTGGAG
    GAGCAGCAGG ATCATGACGG GGTTGTGTCA GCCAGCGGCG GAGGGTCCGA CGAGGAGACA
    CTGGCCCTAC TCAAACGACA GGGTTTACTG CAACCCCAAC ACCACCACCA TCCACACCAC
    CCTCCGATGG CCAGCGGCTC AGAGCAGGCA CCATTCACCT CCCGTGTGGG CCTGCTGTTG
    GTTTTCCCAC TGCTGCAGTC GCAAACTCGG CACGACCCTG CGCTGTGCGG CGTCACAGCG
    GAGGTGTTGC TCGCATGTCT GCGGGACTGC CAGCCACTCA GCCTGGGGAA GGAGCCCGCC
    GACTGTCTTA ATGGCCTGGA GAGGTTGCTG TGCTCCTGGT TGGAGGAGAC GGAGGGTTCG
    GCACCAATGA GGCCACTGCT CACCCAACAG CAGAGACAGA ACGCAGCTGC GGCGCTAGTG
    GCTCTGGCAT GTGCAAGGGG CTCCCTCAAA ACCTTCATCC ACACGGTCCA CCTGCTGCAG
    AAACAAACAG ACCTGGGTCA GCTTCCTGTG GCTGATGTTC TCTATCGACT GCTGCTTCTT
    GAAGGAGGTC CAGGTTCGCC CTCATGTCTG CTGGGGGGGA AACATATTGT GTCGTGGGGC
    TTTGAGGACA TGCTGCCCAC ACCAGAGAGC AGCGCCGCAG GAGCCGACAG TAAAGACACA
    GATCTGGGCC GCAGCCTGGC CACAGATGGA TTCTATCTCT ACACCACGAG CTCTTTTGGG
    AAGGGTTTGA GCAAGCTGGG CTCCGGGCAG CACGGGACGC TGAGGGGTTT TGTGTACTGT
    CGTAATGAGG AGATGGAGGC GGGCTGGGTG GCTCACAGCA GTGGATTTCT GCTCCACCGT
    CCTGCATCAT TTGACACTAA ACCTCATCAG CTGTGCCACG TCATCGACCC GTTCACACTG
    CAGGTTTCTC AGGTGTTAGC GATGCCCGTT CATCATTTCC CAGTGGGCAG CTGCCTGACC
    ACGCTGCGTC TGTGTTCTGA CGGCACATAC TTGTATTGGG TTTGGTCTCC GGTCAGCATC
    AACGAGAAAA CGCAGAAAGG CCACTCTGTC TTCATGGACG TCTTCCAGTT AACGAGTGAG
    TCCGGTGTGT GTGTGGCCAG TCCTGTACAA GACCGGGTGA TCCTGTTGCG AAAGGAAGGC
    GAGTCGTCCA AGTGCTTGAA TGAGTTGCTC CTCAGCCGAA TGTCGCGCTT CCGAGCCTCG
    CACTCGTCCA CACTGGCGGC TCTCACCGGC TCCGCCATCA CCAACCCTGT CAAAGAGGAG
    CAATCGGTGG TCAACACAAG CTGTGGTCTG TCCATCAAAA CACTGAGGAA GACACCCATG
    TATGTGTGCG GGACGTCCCT GGTGATGCTG GTGCCGACTC CAGGTGTTGC TGGGAGCTCC
    GCCACGCGCT CGCTGTTCGG GGGATCCAGT GGCCTTTCTT CTCTCAAGAT CTTGGCCTCT
    AGTTTGGTGT TTAACCTGGC GGACGGTCAG TTCAGCAATC GCGTGGATCT GGTCGACGCT
    CCGGGAAGTT CTCTGGGTCG TGGAGCTCAG GTTGCAGGAC TCGGCGCCTG TTATGATGCA
    CTGAACAACT TAATCTGGAC GTGTTCGAGC GACTACATGG ACCAGTGGTG CAACCCTGGA
    AATCAGGCCT TCCATCATGT GTGCCAGCGA CTCGGCGTCA GTCACGCCAT CAGCCAGACC
    AAAGAAGAGA TGATCGACAC ATCCAGCGTG ATCAGCCAGC TGCTCCATCA TGTGGGAGCC
    ATGTGTATCC ATCAGCTGAA CCTGCTGGCC GCCAGCAGTA GTTTACACTT GGGCACCTTC
    ATAGGGAAGC AGCCAATGGA GGCCTGTCAC TTCAGCTCCA TCTGTGACGT GATGGAGAAA
    GCCATGGTCA ACGGAGACAC CCGCATCATC CGCTGCATCC TGGTCGTTTT TCAGGTGGTT
    TTCCAGTTTT TTAATCCAAA CTCAGAGCAG AACCGGGAGT GTGTGAAGCG CTCAGGACTG
    CTGCTGTGGC AGTTACTCAT GGCTCCACCT GATCAGATCC AGCCGGAAAT ACAGAAAGAA
    GTTTGTCTCG CCATCAGCTC AGGTTTAAAC ACCCTGTATC AGAGCGAATC TGAGTTGAAC
    AACCTGCTGA AGTTTGTTTT CACAGAGGGG GAGAAGAACA GTGGTTTGTC TCAGTTACGA
    GACGTGATTT TAAACAATCT GGCTAACCAG TTACAGAAGA ACCGCTTTGG TAGTGAAGAT
    GATGATCACT ACAGGCTGAA GGATGAGCTG CTGCAGTGCA TCTTGAAGAC GGTGGTGCGC
    GAGTCGTGTC TCCTCATCAC CAAATGTCAA ACCGTTTCCA AAGAAGACCT TCAGAAGCTG
    CTCTCTACTG TACCTGTGGT GTCTCCCAGC TTGCGTTATT TAATGGCTGT GCAGAATCAT
    CTGCTCAGCA ACACCATCCT GCTGCACCCG GAGGACATGG AGGACAGCGA CAGCTCCCTG
    CAGGGGGAAA CAATGAAGGT ACAGGAGCTG CAGACGAGCA TCTTGTCTCT TGCGTTGAAG
    ATTCTGGTTG GATGTGATGA AGTTCTGGAG ACGCTTCAGC AGGTGACCAG CTCTCTCATC
    AACAGCAGCA TCAGCGACCG AGAGACCAGG CTCAGAGGTC TTGAACAGGT GACCAAGGCC
    ACCATGTTGG GTCATTTGCT GCCGGTTCTG CTGACGTCAC TGATGCACCC GAACCTCCAA
    ACACTCACGC TAGCAGATGC GCTCATGCCA CAGCTAGTCC AGCTCGTCCT CTACACTAGC
    CAGACCGCTT TGCTGCTAAA GACACATTCT CCATTATTTG TTGAGGATTC CCCAATAAAT
    CAAGGCTCCA GCCAGCCCTC ATTAAATGAC AGTTTTCAGA TTCTGGAGGA GAGAGAGGAG
    CCTGGCTTTC TGACGGGGCT GAAGATCCCC GCCCCGTGGG CTGCTGGGAA AACGGTGGAA
    ACTGTTCACC CGGTGCGGGA TAACTATAAA TTTAAAGACA CAGTGCACAT TCCTGGAGCT
    CGCTGTCTGT ACCTGCGCTT CGACCCGCGC TGCTCCTCAC AGTACGACTA CGACAAGCTT
    GTGATTTACG CCGGGCCGAA CACAAACAGT CGTAAAGTAA GCGAGTATGG AGGAAACACC
    ATGGGTTATG GCAGCCGCAG TGTATTGGGA ACCGGATGGC CAAAAGATCT GGTCAAGGTG
    GAAGGAGATA CGGTGACGTT CTCTTTTGAG ATGAGAAGTG GACGCGAGCA CAACACTCCA
    GATAAAGCCA TGTGGGGTTT CTCGTGCACC GTCAGAGCGC AGGAGTCGTC TGAGGATGTG
    TCTGGCGGTC TGCCGTTTCT GGCTGATCTG GCTCTGGGTT TATCAGTGTT AGCCTGCGCT
    ATGCTACGAA TCCTCTATAA TGGGCCTGAA ATCACACGAG AGGAAGAAGC TTGTCACGAC
    CTTCTCTGCT CCAAATTACT GCAGAGGTGT CAGTGGCAGG TGGAGGCGAA CGGAGCCATC
    TCTCCTGCAC TCACGCCATC TCCGTCTCCT CTTCCTCTGA CTATCGATGA GGAGCGCGAG
    TTCACATATC CATCAGATAT CCTCGCTCCG CCGCCGGGGC CCATCCCGAG CGCCTGCTGT
    GAGCTGCCCC GCATTCACTT ACCGCAGGGT ATCATGGGCC GCCTGAGGGA GCTGTCTGGA
    CGTGCTCGGC CACAGTTTCG CCCCAGCATC AAAGAGGTGA TCCGTCCAGA CGTGATGGAG
    GAAGTGATTG TGTCGTGTGT GATTAAACAC TTGGGTCTGG TGGACGCGCT GCAGTCGCTG
    GTCAACTTCC AGTACAGAGA ATCTCACAGA GAAGAACACA ACCTGCTCTG CAAGATCATG
    GCTGAGACCT TCAAGAAGAT CAACGCCATG GAGAGACAGT TACAGAGCGT GGCTGAGCTG
    GAGCAGAAGT GGCAGAATGA AGTAGATGAA GCTCAGCAGG GAAAACTGGA GAACAACACT
    CCTTTCTTCC ACGATTATCA CTTCTTTGAG AATAAAATGA AGGAGCTGGA GCTTCTCTGT
    TCGCTAAAGG AGGTTCCTCT GGACTGCACT GATCCAGAAA ACGCTGTGAT GGCTTTGAGA
    GAGAAGTTTT TTCATGAGGT GAACTCCACG CTGCAGCAGC AGAGAAGTGT TGTTCCTCTG
    GCTAAAACCA AAGCGCTTGT GAAGAGTTTG ATCAACCGCT CGGAGCTGCT GCTTCATGTC
    ACCATCGCTC CGCAGTGCAA GAGTCTGTCA TCAACACCCA CAAGCACACC GGCCTGTAAG
    TCGGTGTCAG ACGCTAAATC GATGGCTCCC GTGGTGAAGC AGCCGGTGTT CCTGCGCAGC
    ATGTCTGCTC CGTCAGATCT GGAAATGATC GCCAGCAGTG ATGTGGAGTT CACACACGGA
    GCGCAAAGGA GGCGGCAGCA CCCCTCCAGC CACCGGAGCA GCTCATTTAC ACTCCTGCAG
    TCATTGGTGA TTGAAGACAG TAAAGATAAA CCCACGTACA GTGTGCTGCT GGGACAACTG
    TTCGCCTTTA TAGGAACCAC TCCTGACCAA ACCGTGTCGA GCAGCAGTTT TCTGTCCGCA
    GCTCAGACGC GCTGGAGAAG AGGACGAACA CGCAAACAGG CTCTGGTTCA CATGCGGGAG
    CTGCTGACCG CTGCCGTCCG CGTCGGTGGA GTCACTCATC TGGTCGGACC CGTCACTATG
    GTCCTGCAGG GGGGACCCAG GGTGGAGGAG CTCACCTGTG GTGGGATGAT GGAGCAGGTG
    CAGGAGGCGT TTGGAGAGAC CATGACGTCA GTGGTGTCTC TGTGTGCCCG ATACCCCATC
    GCCTGTGCCA ACAGCATCGG CCTGCTCTGC ACCATCCCCT ACACCAGGAG TGAGGAGCAG
    TGTCTGGTGC GCAGCGGTCT GGTTCAGCTC ATGGATCGCC TCTGCAGTCT GAGCAGTCAG
    AGAGACTGCA GCTCCAACGA GAAGCAAACC AAAAAGCAGA AAGTGGCCAC CATGGCCTGG
    GCTGCGTTTC AGGTGCTGGC CAACCGCTGC ATTGAGTGGG AGAAAGTCGA AGGAGGCTCC
    ACAGACGCAG TGCATTCAGG GTTGGCTCGA CAGGTGTCCA GCCTTCTGAC CAATCATCTG
    GCTCGAGCGA CAGACTGCTG TGGAAACCAG GCGGCAGGAA ATGATGCGCT GCAGGATGTG
    CTCAGTTTAC TCAACGATCT GTCCAGGAGT CACATAGGCA AGACCATCCT GAGCCAGCCT
    GCCTGTGTGT CCAAGCTGCT GTCTCTGCTT CTGGACCAGC GACCGTCGCC CAAACTGGTG
    CTGATCATCC TGCAGCTGTG CCGCGCCGCG CTGCCCCTCA TGAGTGTGGA GGATTGTGGA
    AATGTAGCGC TGCCGTCCTG GAGCTACTCC ACAAACGCAC TGGACGCCGA GCAGCAGGAC
    GCCAGCGACC CTGCCTTCCG CATCGCATCT CTGCTGCTCG CCAAACTGGC CGACTATGTG
    GTGCCAGGCT GTCAGACGTT GCTCTCTCCC AGCGCCTCTG AGCCGGACAC CAGTCTGAGC
    CGAGCCTGTC CCAAGAGCAG CTCTAAGACC GACAGAGATG CCAGCGAGGA GGGCGAAGCG
    GTGGACGGAA AACTCTCCAT ATTCATCCAT AAGAGAGAAG ACCAGTCGTC GCACGAGGTG
    CTGCAGCCTC TGCTCAGCAG CTCAGAGGGT CGACCGTTTC GTCTCGGCAC TGGAGCCAAC
    ATGGAGAAGG TGGTGAAGAT GGATCGAGAC ATGACCAAGA GCGGCTGCTG TGAGGTCATC
    ACTGAAGAGG CGTCCGCTGC TCTCAGGAAA GCCAATAAAT GGGCTCAGTC GGGGCTCATT
    GTGAGTGTGG GTCCGCCCAT CGAAAGCAGC GCTCAGGAGA ACTCAGGAGC GACCTCTACA
    GGAGACAAGA AGAAAAGCGC TCAGTCCACG GTGTGTCGAG AGAGGAACGC TGAGCTCGCC
    CGGTCTGACC CGGTGCGTCC GTTCATCAGT GGTCACGTAG CCAACAGTAT GGCAGCAGAG
    GTCATCGCTC TGCTGCACAG CCTCCTGACG GCCCCCGAGT CCAACACTGC TCAGATCTGG
    ACAAGCACTG CAGAAAAGGT GTTGTCCAGA GCTCTGATGT TCATCCCTCA GCTGGGGAAG
    TTTGCTGAGA GTATTCTGGA GAGCGGAAAC AGCAGCGGCA GGAAGTTGTC CAAGCTGCAG
    GCCATTGGTC GACAGGCGGT GGCGGCTCTC TGTGCGCTCG GCGGATTCAA GGAGACCATC
    AAGATTGGAT CTGAAGTGCA GGTGGTTGGT AAAGGTGTGT TGGACAGTGT CGGTGTGGTT
    ATATCCATTA ATGAGCAGGA GGGCATCGCC ACCGTCAAGT TTCCATCCTG CGAGTATCGG
    CGCTCCAGCA AAGCATCTGA TATCCTGACG GTGCCCATAT CACGCCTGTG CACGCCGCGC
    TCAGAGGCTC TGCCGCTGCA CAAGCTTTCC ATCACAGAGA AGGTTGTTCA GGCCGTTCAG
    TCCATGTTGC TGCCGCAGGA GGGCAGTCTG TCCATACACA CGTCTCTGCC GGCGTCTGGA
    GACGGCTCCA GTCCTGTGAT GGCAGTGGTG CGTCTGCTGG CCGAGATCAG AACCAGAGCG
    TGTCTGGTGA TGGCGCAGCT GCTGGAGGAC AGCTCTTTCT GTGAGGAGTT TATCCAGCAG
    TGTCCTGCAG CCGTGGAGGT GCTCAACCTC ATCGCTCAAG AGTGCAGTCC TGGAGAGCGT
    TTGGTGATGG TGGAGGCTCA GTGTGAGCGT CTGCGCATGC TTTATCGAGA CTGTGCTCGT
    CCTCCACCAC CGCCTGTGCA AACTGACCAA CGGCAGCCGA AGGAGATCAC GTGGTGCCCG
    TCTCGAGTCT TCCCTCCGGT TCGGGCCTGT ATGTTTTCCT CGCACTTGAC GTCGGTGACG
    TTTCTGGCTG ATCCGAGTGC AGGCGGAGGC CTTCCCAGAG GAACCTTCAT CTACGCCACT
    TCACCCGTGC CCATTCAGGC TCCGTCTTTT TACTGGGAGA TCGAGATCGT TTCGTTCGGA
    GACTCGGAGG ACGACAGCGG TCCAATAGTG TCCTTTGGAT TTTCCCCGGA GGCGGAGAAG
    AGAGACGGAG CCTGGACCAA TCCCGTCGGG ACCTGCCTGT TTCACAATAA TGGCCGGGCG
    GTTCACTACA ACGGCTCCAG TTTACTGCAG TGGAAAAGTG TGAGGCTCGA CGTGGCTCTG
    CTTCCTGGTG ATGTAGCCGG CATCGGCTGG GAGCGTCTGG AAGGCACTCC TCCTCCACCC
    GGTCAGCCTC CCAAAGGAAG AGTGTACTTC ACCTACTGCG GTCAGCGACT CGCTCCCTAC
    CTAGAGGATG TGGCTGGAGG CATGTGGCCC GTCGTTCACA TCCAGAAGAA GAATACGAAG
    ATTCACGCCA ACTTCGGCTG CCGGTTGTTT GCGTACGCTG AAGGCCAGGC TCACAGAAAC
    GCTGCTGATC TCTGCATGGA TCTGTCAGAG GAGATCAGTG CTAACTTCGA GGCTCTGCCC
    TTCGCCATGG CCTCCGACAG CGATAATGAT GCGGGCACCA GTGTGGCGTC TGACTCCGGC
    TCCCACGGGC CTCCTTGTCG CATCGCTGCC GTCGTCACAG CACAACAGCA ATACAACAGC
    GATGCTTCTT GCCACTACAA GGTAGAGCTG AGCTATGAGA ATCTGGTGGT GTCGGGACCG
    AACGCTCATC CGCCACCCAT CGCTGATGAC GAGAGCGATG ATGAGGATGA TGAAGACATT
    CCCCGGGAGG ATCATTACGC CCTGCTAGTG AAAGCGTGGG AAACTAAAGT GTTTCCCACC
    ATTCGCCGGC GGTTCAGGAA CGAGGCGGAG AGGAAATCTG GCCTGGATCA GATCAAGGGA
    GCGCTGCAGC TCGGTATGGT GGACATCGCT CGTCAGACGG TTGAGTTTCT GTATGAAGAG
    AATGGCGGGA TCCCTCGAGA CCTCTACCTT CCAACCATCG AAGACATTAA GGATGAAGCA
    AACAAGTTCA CCATCGACAA AGTCCGCAAA GGCTTGACGG TGGGCATCCG CTGTCCAGAG
    GGAAACAACA CCTGCAGCAG CACAGGAGGA ACCGCTCTAC CAAAGTTTGC CATCCGCGGC
    ATGTTGAAGA CCTTCAGCTT ACATGGAGTT GTGCTCGATG TGGATTCGGG TAATGAGCTG
    GTGCAGGTGG AGACGTATCT GCGCAGTGAG GGTGTGTTGG TGCGCTACTG GTACCCCATA
    GAGATGCTGG AGAGACCGCC GGCAGGATCC CGCAGGGCTG CAGCCAATGG CCTGGTGTCT
    TTGGACAGCA GCAACATCCA GATTCATCGG GAGCTGCTGC GCTGTGAAAG TGCTCTGGCG
    CGTCTGTACT GCCGCATGGC GCTGCTCAAC ATCTTTGCGC CCAAGAGTCC TCACACATTC
    ACGCGCCTCT TCCACATCCC TGCAGTGCGT GACATCACAC TCGAACACCT GCAGCTCCTG
    TCCAATCAGC TGCTGGCTCC GCCTCTGCCA GACGGCCGGA TCAGCTGTAA CTCTGTGCTG
    CTGGCTCAGT CGGTCCTACA TGAACTGCAG GGTCAGAGCT GCTCTCCCAC TGAGCTCTTC
    TATCAGGGAA ACGCTCAGAG TGTCCGCGAG TGGCTGAGCG TGGCCATCAG CAGAGCTCTG
    CATCAGGGGG AGGACAGTCT GCTGGAGCTC ACCAAACAGA TCTGCTGCTT CCTGCAGAGT
    GCGCCTGAGC AGTTCACGCT GGAGGAGATT CCCATCACCG AGTCAAAGGT CAGCATGGAC
    GTCAACTTCC CCGGCGCTGC GTTTGTGCTC GTCTCCTGCA AAGAGAGTCA ACTCAGCTGC
    CGCAAAGATT CCTCTCTGTA CAAAGCTCCA TGGGCTCGAG TGATGGTGTA TGGGCTCGGT
    CATAAAGTGC GGCGTAACGG CCAGCTGAAC CTGATGGAGG CCGTGTGTTT CCCGCTGGAC
    GCCTCTCCGT CCAACACGGG CCTCACTCCT CCACCCACAG CCAACCAGTA TCCCAGCATC
    ATCATCCCCA CCGACAGAGT GCACGTCAAA CTGGGTGTGT CTCCCCCTCC TGGAGCAGTG
    CTGGTGTTGC ACTCGTTGCC TCTTGAGTTT CCATTGGCGA TGGCCTTCGC TGAACAGCTG
    CTAACCTACA GTGTAGGAGA GACCGTTGAG CGCTCTGAGG ATGAGCTGGA CACGGTGCCC
    ACCTCTGTGC TCATGCAGGT CGTGGAGCTG CTAGGTGGCC TTTTGTGGAC CACAGATCTT
    GCTCCGAGCA TCAAGGAGCT GACCTTCCAC CTGCTTGCAG AGCTCCTCCG CAAGATCCAC
    GCTTTTGAGC GCCGCAAATC CCCTTCGGGT CTCTCTAGCC ACATCACGCT GCTTCTCAAC
    CCCTGCCTAA CAGTCCTGAT GGTTCTTCAA ACCGAACTAC GAAAGCTTTA CGACCACGAG
    ACCCAAAGCT GGGGGTTGGT TAGCGGAGGA TCCTGCACTT CCGCAACAGG CGGCAGTGGA
    GCGCTTGCAC TTTCTGCAGA GCGGAGCCGC TTCTCTACAT ACTTCCACGC ACTGATGGAG
    GTTTGCCTGG CAGTGGCGGA AGTCACTTTA CCGCTGAGTT CTGGTTCTGG GACGTCAGTA
    ATCGGCGTCC CTTCATCAAC AAGCACAGCC AACTGCAGTG ATTCCTCATC CTCTTCTTCG
    TCCTCTCCAG GCCAGACGCC GCAAAGTCCG AGTTTGCTTT CAAAGCGCAA AAAGGTGAAG
    CTAAAACGTG AGCGCGCTGC AGTCGGCTCA GCTTCCGCCG TCTCAGGGAA AAGTGGCTCG
    AGATCATCAG AATGCGACAA TGCTCTATTA AACATGAAGC CTGAAGACAT GTTGTGGTTT
    CACCGGGGGT TGACCTTGCT TTTGATTTTA CGGCATCTTA CTAATAAGGA CCCGCAAGGT
    CTTGGCGTCA CCGGCGACGC TGTGGCAGAC GCTTGCCAAG CTTTGGTTGG CTCGACAGCT
    CACAGTCGCT TGCTGGTGCT CTCAGGAATC CCGACTCACC TGGAGGAGTC TGTGGTGCGC
    AATGCAATAC GAAGAGCATG CAATGCTCAT GGCGGGCTCT TCAGGGATGA GATCTTCATT
    CCAGTTCAGG AAGAAGACCC TAAAAAAGCC AAAGCGGAAA CGACATGTGT TCCTCAAGAT
    GTAAAGAGAG AATGCACTGC AGGAAGCCCC AACAGCAACA GTGCGGCAAT GAGTGTCTCC
    AGCGCTTTAC AGGCAGCCTT CAGAGCCTCC GGAGAGCCCT TGTCCAATCA GGAGATGGTA
    AACAGTCCTT CCCAGCAACA AGCACCTCTG GATCCACTCA CAGTGTCCAG TCAGGAGAGT
    CTGGACGTGT CTCTGTGTAG CACAGAAAGT CAAGGGAGTC TCGGCAGCCT CGGGGAGCAG
    GATAGTGCTG CCACGTCAGT CTCAGATGGG AGCTCCATGT ACACGGTGGC CTCACCTGAT
    GTTCATGCCA GTGTGACGCG TCCCATAAAA GGCTATGCTG TCATTGAGGT AAGGGCACGA
    GCTCAGGTGG AGAAGATTCG GGCCAGTCTT TTCAACAGCA GCGATCTGAT TGGCCTCTCC
    AATCTGGAAA GGGAGGAGGA GCTAATGGAG ATGACCAATG AGGAGATCCT CACCGCCTCC
    AGTGTCAATC AGAGTTTATT TGACACACAG GGCAGCTCTG CTCTGGAGGA GTACTTCCTG
    GATAAATCCA TCAGAGGAGA CAAGCTGGTT CCTGGAGCTC GTGACGTGCT GACAGACATC
    TTTAAGAGCT GCATATACGC AGAGCAGGCG CTGAGCCCCG TTCCCGTGAA ACCTGTCAAA
    GTCTCAGACA TTTACCTCAG CAAAGAGCAG ATCAACTCTC AGTCTCCCGG AAACCTGCTA
    CACGTGTTTT TCACACACGT TCGGCCTCCC AAAAAAGTGC TCGATGACCA GCTCACACAG
    ATTCTGCGGA AATACGGCAC AGTAAAGCCC AATAAAAACA AGCACAGCAA AGCGGGCAAA
    GAGCAGCACC AAGGCAAAGT GGTGAGCACA AAAAGACCCA TCACCAAACC TCCGGCCAAA
    GAGAAGTCCA TGCTGAACAG CGTGCGAACT GTACTGAGTG AGAAAAAAAC CGTCCTCAAG
    CCAAAATCTC CAGAGAAGAC CAAACCCGAT GAAAAAGACC CCGAAAAATC TCCTGCCAAA
    AAACCCGAAG TGCCAGAGGA GAAGTTTTTG ACGCTGGAGG GTTTCCACAA GTTTACTCTG
    GACAGAGCCA AGCAGGATAT CCGCAGTGTT TGGCGGGCGA TCCTGGCCTG TGGATATGAC
    CTGCACTTTG ACAGATGCAC ATGCATTGAT TCCCGACATG CTCAGAAAGC CAGCAGAAAA
    TGGAGCTTCG AGATGGACTT TGCGTTGGTG CAGTTTGTGA ACCGTCTCTG TCGTCACCTG
    GCCATCACTC CTGCCCGCCT GCATCCACAT GAGGTTTACC TGGACGCCAG CAAAGATGGC
    GCGGATCCTG AGGTCGCGTG TCTGTTAGGT GTCCCAGTGG AGAGTTTGCG TCTGCGCTTT
    GCTCTGCTGC AGTCCCTCAA CAGCACGCTG GAGAGCTTCT TCCTGCCTTT AGTGGAGCTG
    AGACTCACAC AGACCTACCA GAACAGCATC GCCACACTTC TGTGCGAGGC CAAAGGCCTG
    ATATTTTACG ACACTAAGGT GACGGTGATG AACAGGGTGT TGAACGCTAC CGTGCAGCGG
    ACGGCAGATC ACGCAGCGCC TGAAATCACT CTCGACCCGC TGGAGATTGT TGGAGGAGAG
    ATGCGCGCTG CGGAGAACAC TTATTTCTGC CAGGCAGCGC GGCAGCTGTC GTGTGTGCCA
    TCGTCTCAGC TGTGTGTGCG CATCGCCAGC GGAGGAGACC CCACATACGC CTTCAACATC
    CGCTTCACCG GAGAGGAGGT CCACGGCACC AGTGGCTCTT TCAGGCATTT CCTATGGCAG
    GTGTGTAAGG AGCTCCAGAG TTCAGCTCTC TCTCTTCTTC TGCCCTGCCC GAGCGCCGCC
    GCTAACCGCA ATAAGGGGAA GTACATCCTG ACTCCGTGTC CCATCACATA CGCTGAGGAG
    CAGCTGCTGA ACTTCTTCGG GCAGCTTCTG GGCATCGCCA TCCGGGCCGA TGTGCCGCTG
    CCTCTGGACC TGCTGGGATC CTTCTGGAAG GGTCTGGTCG GAGAGGCTCT CGATCCCGAA
    GCTGACCTGC GGGAAGCAGA TCTGCTCACG TACAGCTACA TCAAGAAGTT TGAGAGTTTG
    TGTGATGAAG CGGAGCTGGA AGCGCTGTGT GCTGAAGTGT CGTCTCAGAG TCCCCTGGGG
    GAGAGTCCAG ACTCCCCCAG CCGGCCCTGC TGCACCTTCA CATACATCAC TATGACTGGA
    GAGGAAGTGG AGCTGTGCCA GGGAGGACGT GACATCGCTG TCAGCTGGGA GAATAAGGAT
    GTGTATGTGC GAGCGGTGCG TGAGCTGCGT CTGCGGGAGC TGCAGAATGT GGAGTGTATG
    TCTGCGGTGC GCGCCGGTCT GGGCTCCATC ATCCCACTGC AGCTGCTGAC TTTACTGAGT
    CCCGCAGAGG TGGAGCTGCG CACCTGCGGT CTGCCGGACA TCAACCTGGA GTTCCTGAAG
    GCGCACACCA TGTATCAGGT GGGTCTGATG GAGACGGATC AGCATATCGA GTTCTTCTGG
    GCGGCGCTGG AGATGTTCAC GCAGGAGGAG CTCAGCAAGT TTATTAAGTT CGCCTGCAAC
    CAGGAGAGGA TTCCCTTCAC CTGCCCGTGC AAAGATGGGG GTCCGGACAC CGCACATGTG
    CCCCCCTATC CCATGAAGAT CGCCCCGCCG GACGGCACCG TGGGCTCTCC AGATCAGCGC
    TATATCCGCG TGGAGACGTG TATGTTCATG GTCAAGCTGC CGCAGTATTC CTCCCTGGAT
    GTGATGCTGG AGAAACTGCG ATACGCTATT CACTATCGAG AAGATCCTCT GAGCGGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa51539
    Clone ID Related Accession (Same CDS sequence) XM_009303668.3 , XM_009303668.2
    Accession Version XM_009303668.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13260bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303668.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
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    121
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    301
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    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
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    3901
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    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
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    4561
    4621
    4681
    4741
    4801
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    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
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    6721
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    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
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    7741
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    7921
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    9061
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    9301
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    9421
    9481
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    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGCTCAG GTGCTGTGGA TTCTTCCCAG TGGCTGTCAG TGAAGGAAGA GACTATTTTC 
    CTTCACGATG GACTAATTCG GGTCACGGAC CTGGCTGAGC TGCCCAGCGA AATCGGCGTG
    TCTGAACAAG CTGAATCCGA GCAGGAGATC TTGACGTTTG AGACCAAGAA TCCAGTGGAG
    CTAGCCGAGC GTCTCCGAGC CGTCTGTGGA AACCAGAGCA ACGCTTATGC ACGCCTTTTG
    GAGTATCGTC TGAATGCACT GCGTGGCCTG TGGGGGGCGC AGCGTCAGCT GGCCCTGGAG
    GAGCAGCAGG ATCATGACGG GGTTGTGTCA GCCAGCGGCG GAGGGTCCGA CGAGGAGACA
    CTGGCCCTAC TCAAACGACA GGGTTTACTG CAACCCCAAC ACCACCACCA TCCACACCAC
    CCTCCGATGG CCAGCGGCTC AGAGCAGGCA CCATTCACCT CCCGTGTGGG CCTGCTGTTG
    GTTTTCCCAC TGCTGCAGTC GCAAACTCGG CACGACCCTG CGCTGTGCGG CGTCACAGCG
    GAGGTGTTGC TCGCATGTCT GCGGGACTGC CAGCCACTCA GCCTGGGGAA GGAGCCCGCC
    GACTGTCTTA ATGGCCTGGA GAGGTTGCTG TGCTCCTGGT TGGAGGAGAC GGAGGGTTCG
    GCACCAATGA GGCCACTGCT CACCCAACAG CAGAGACAGA ACGCAGCTGC GGCGCTAGTG
    GCTCTGGCAT GTGCAAGGGG CTCCCTCAAA ACCTTCATCC ACACGGTCCA CCTGCTGCAG
    AAACAAACAG ACCTGGGTCA GCTTCCTGTG GCTGATGTTC TCTATCGACT GCTGCTTCTT
    GAAGGAGGTC CAGGTTCGCC CTCATGTCTG CTGGGGGGGA AACATATTGT GTCGTGGGGC
    TTTGAGGACA TGCTGCCCAC ACCAGAGAGC AGCGCCGCAG GAGCCGACAG TAAAGACACA
    GATCTGGGCC GCAGCCTGGC CACAGATGGA TTCTATCTCT ACACCACGAG CTCTTTTGGG
    AAGGGTTTGA GCAAGCTGGG CTCCGGGCAG CACGGGACGC TGAGGGGTTT TGTGTACTGT
    CGTAATGAGG AGATGGAGGC GGGCTGGGTG GCTCACAGCA GTGGATTTCT GCTCCACCGT
    CCTGCATCAT TTGACACTAA ACCTCATCAG CTGTGCCACG TCATCGACCC GTTCACACTG
    CAGGTTTCTC AGGTGTTAGC GATGCCCGTT CATCATTTCC CAGTGGGCAG CTGCCTGACC
    ACGCTGCGTC TGTGTTCTGA CGGCACATAC TTGTATTGGG TTTGGTCTCC GGTCAGCATC
    AACGAGAAAA CGCAGAAAGG CCACTCTGTC TTCATGGACG TCTTCCAGTT AACGAGTGAG
    TCCGGTGTGT GTGTGGCCAG TCCTGTACAA GACCGGGTGA TCCTGTTGCG AAAGGAAGGC
    GAGTCGTCCA AGTGCTTGAA TGAGTTGCTC CTCAGCCGAA TGTCGCGCTT CCGAGCCTCG
    CACTCGTCCA CACTGGCGGC TCTCACCGGC TCCGCCATCA CCAACCCTGT CAAAGAGGAG
    CAATCGGTGG TCAACACAAG CTGTGGTCTG TCCATCAAAA CACTGAGGAA GACACCCATG
    TATGTGTGCG GGACGTCCCT GGTGATGCTG GTGCCGACTC CAGGTGTTGC TGGGAGCTCC
    GCCACGCGCT CGCTGTTCGG GGGATCCAGT GGCCTTTCTT CTCTCAAGAT CTTGGCCTCT
    AGTTTGGTGT TTAACCTGGC GGACGGTCAG TTCAGCAATC GCGTGGATCT GGTCGACGCT
    CCGGGAAGTT CTCTGGGTCG TGGAGCTCAG GTTGCAGGAC TCGGCGCCTG TTATGATGCA
    CTGAACAACT TAATCTGGAC GTGTTCGAGC GACTACATGG ACCAGTGGTG CAACCCTGGA
    AATCAGGCCT TCCATCATGT GTGCCAGCGA CTCGGCGTCA GTCACGCCAT CAGCCAGACC
    AAAGAAGAGA TGATCGACAC ATCCAGCGTG ATCAGCCAGC TGCTCCATCA TGTGGGAGCC
    ATGTGTATCC ATCAGCTGAA CCTGCTGGCC GCCAGCAGTA GTTTACACTT GGGCACCTTC
    ATAGGGAAGC AGCCAATGGA GGCCTGTCAC TTCAGCTCCA TCTGTGACGT GATGGAGAAA
    GCCATGGTCA ACGGAGACAC CCGCATCATC CGCTGCATCC TGGTCGTTTT TCAGGTGGTT
    TTCCAGTTTT TTAATCCAAA CTCAGAGCAG AACCGGGAGT GTGTGAAGCG CTCAGGACTG
    CTGCTGTGGC AGTTACTCAT GGCTCCACCT GATCAGATCC AGCCGGAAAT ACAGAAAGAA
    GTTTGTCTCG CCATCAGCTC AGGTTTAAAC ACCCTGTATC AGAGCGAATC TGAGTTGAAC
    AACCTGCTGA AGTTTGTTTT CACAGAGGGG GAGAAGAACA GTGGTTTGTC TCAGTTACGA
    GACGTGATTT TAAACAATCT GGCTAACCAG TTACAGAAGA ACCGCTTTGG TAGTGAAGAT
    GATGATCACT ACAGGCTGAA GGATGAGCTG CTGCAGTGCA TCTTGAAGAC GGTGGTGCGC
    GAGTCGTGTC TCCTCATCAC CAAATGTCAA ACCGTTTCCA AAGAAGACCT TCAGAAGCTG
    CTCTCTACTG TACCTGTGGT GTCTCCCAGC TTGCGTTATT TAATGGCTGT GCAGAATCAT
    CTGCTCAGCA ACACCATCCT GCTGCACCCG GAGGACATGG AGGACAGCGA CAGCTCCCTG
    CAGGGGGAAA CAATGAAGGT ACAGGAGCTG CAGACGAGCA TCTTGTCTCT TGCGTTGAAG
    ATTCTGGTTG GATGTGATGA AGTTCTGGAG ACGCTTCAGC AGGTGACCAG CTCTCTCATC
    AACAGCAGCA TCAGCGACCG AGAGACCAGG CTCAGAGGTC TTGAACAGGT GACCAAGGCC
    ACCATGTTGG GTCATTTGCT GCCGGTTCTG CTGACGTCAC TGATGCACCC GAACCTCCAA
    ACACTCACGC TAGCAGATGC GCTCATGCCA CAGCTAGTCC AGCTCGTCCT CTACACTAGC
    CAGACCGCTT TGCTGCTAAA GACACATTCT CCATTATTTG TTGAGGATTC CCCAATAAAT
    CAAGGCTCCA GCCAGCCCTC ATTAAATGAC AGTTTTCAGA TTCTGGAGGA GAGAGAGGAG
    CCTGGCTTTC TGACGGGGCT GAAGATCCCC GCCCCGTGGG CTGCTGGGAA AACGGTGGAA
    ACTGTTCACC CGGTGCGGGA TAACTATAAA TTTAAAGACA CAGTGCACAT TCCTGGAGCT
    CGCTGTCTGT ACCTGCGCTT CGACCCGCGC TGCTCCTCAC AGTACGACTA CGACAAGCTT
    GTGATTTACG CCGGGCCGAA CACAAACAGT CGTAAAGTAA GCGAGTATGG AGGAAACACC
    ATGGGTTATG GCAGCCGCAG TGTATTGGGA ACCGGATGGC CAAAAGATCT GGTCAAGGTG
    GAAGGAGATA CGGTGACGTT CTCTTTTGAG ATGAGAAGTG GACGCGAGCA CAACACTCCA
    GATAAAGCCA TGTGGGGTTT CTCGTGCACC GTCAGAGCGC AGGAGTCGTC TGAGGATGTG
    TCTGGCGGTC TGCCGTTTCT GGCTGATCTG GCTCTGGGTT TATCAGTGTT AGCCTGCGCT
    ATGCTACGAA TCCTCTATAA TGGGCCTGAA ATCACACGAG AGGAAGAAGC TTGTCACGAC
    CTTCTCTGCT CCAAATTACT GCAGAGGTGT CAGTGGCAGG TGGAGGCGAA CGGAGCCATC
    TCTCCTGCAC TCACGCCATC TCCGTCTCCT CTTCCTCTGA CTATCGATGA GGAGCGCGAG
    TTCACATATC CATCAGATAT CCTCGCTCCG CCGCCGGGGC CCATCCCGAG CGCCTGCTGT
    GAGCTGCCCC GCATTCACTT ACCGCAGGGT ATCATGGGCC GCCTGAGGGA GCTGTCTGGA
    CGTGCTCGGC CACAGTTTCG CCCCAGCATC AAAGAGGTGA TCCGTCCAGA CGTGATGGAG
    GAAGTGATTG TGTCGTGTGT GATTAAACAC TTGGGTCTGG TGGACGCGCT GCAGTCGCTG
    GTCAACTTCC AGTACAGAGA ATCTCACAGA GAAGAACACA ACCTGCTCTG CAAGATCATG
    GCTGAGACCT TCAAGAAGAT CAACGCCATG GAGAGACAGT TACAGAGCGT GGCTGAGCTG
    GAGCAGAAGT GGCAGAATGA AGTAGATGAA GCTCAGCAGG GAAAACTGGA GAACAACACT
    CCTTTCTTCC ACGATTATCA CTTCTTTGAG AATAAAATGA AGGAGCTGGA GCTTCTCTGT
    TCGCTAAAGG AGGTTCCTCT GGACTGCACT GATCCAGAAA ACGCTGTGAT GGCTTTGAGA
    GAGAAGTTTT TTCATGAGGT GAACTCCACG CTGCAGCAGC AGAGAAGTGT TGTTCCTCTG
    GCTAAAACCA AAGCGCTTGT GAAGAGTTTG ATCAACCGCT CGGAGCTGCT GCTTCATGTC
    ACCATCGCTC CGCAGTGCAA GAGTCTGTCA TCAACACCCA CAAGCACACC GGCCTGTAAG
    TCGGTGTCAG ACGCTAAATC GATGGCTCCC GTGGTGAAGC AGCCGGTGTT CCTGCGCAGC
    ATGTCTGCTC CGTCAGATCT GGAAATGATC GCCAGCAGTG ATGTGGAGTT CACACACGGA
    GCGCAAAGGA GGCGGCAGCA CCCCTCCAGC CACCGGAGCA GCTCATTTAC ACTCCTGCAG
    TCATTGGTGA TTGAAGACAG TAAAGATAAA CCCACGTACA GTGTGCTGCT GGGACAACTG
    TTCGCCTTTA TAGGAACCAC TCCTGACCAA ACCGTGTCGA GCAGCAGTTT TCTGTCCGCA
    GCTCAGACGC GCTGGAGAAG AGGACGAACA CGCAAACAGG CTCTGGTTCA CATGCGGGAG
    CTGCTGACCG CTGCCGTCCG CGTCGGTGGA GTCACTCATC TGGTCGGACC CGTCACTATG
    GTCCTGCAGG GGGGACCCAG GGTGGAGGAG CTCACCTGTG GTGGGATGAT GGAGCAGGTG
    CAGGAGGCGT TTGGAGAGAC CATGACGTCA GTGGTGTCTC TGTGTGCCCG ATACCCCATC
    GCCTGTGCCA ACAGCATCGG CCTGCTCTGC ACCATCCCCT ACACCAGGAG TGAGGAGCAG
    TGTCTGGTGC GCAGCGGTCT GGTTCAGCTC ATGGATCGCC TCTGCAGTCT GAGCAGTCAG
    AGAGACTGCA GCTCCAACGA GAAGCAAACC AAAAAGCAGA AAGTGGCCAC CATGGCCTGG
    GCTGCGTTTC AGGTGCTGGC CAACCGCTGC ATTGAGTGGG AGAAAGTCGA AGGAGGCTCC
    ACAGACGCAG TGCATTCAGG GTTGGCTCGA CAGGTGTCCA GCCTTCTGAC CAATCATCTG
    GCTCGAGCGA CAGACTGCTG TGGAAACCAG GCGGCAGGAA ATGATGCGCT GCAGGATGTG
    CTCAGTTTAC TCAACGATCT GTCCAGGAGT CACATAGGCA AGACCATCCT GAGCCAGCCT
    GCCTGTGTGT CCAAGCTGCT GTCTCTGCTT CTGGACCAGC GACCGTCGCC CAAACTGGTG
    CTGATCATCC TGCAGCTGTG CCGCGCCGCG CTGCCCCTCA TGAGTGTGGA GGATTGTGGA
    AATGTAGCGC TGCCGTCCTG GAGCTACTCC ACAAACGCAC TGGACGCCGA GCAGCAGGAC
    GCCAGCGACC CTGCCTTCCG CATCGCATCT CTGCTGCTCG CCAAACTGGC CGACTATGTG
    GTGCCAGGCT GTCAGACGTT GCTCTCTCCC AGCGCCTCTG AGCCGGACAC CAGTCTGAGC
    CGAGCCTGTC CCAAGAGCAG CTCTAAGACC GACAGAGATG CCAGCGAGGA GGGCGAAGCG
    GTGGACGGAA AACTCTCCAT ATTCATCCAT AAGAGAGAAG ACCAGTCGTC GCACGAGGTG
    CTGCAGCCTC TGCTCAGCAG CTCAGAGGGT CGACCGTTTC GTCTCGGCAC TGGAGCCAAC
    ATGGAGAAGG TGGTGAAGAT GGATCGAGAC ATGACCAAGA GCGGCTGCTG TGAGGTCATC
    ACTGAAGAGG CGTCCGCTGC TCTCAGGAAA GCCAATAAAT GGGCTCAGTC GGGGCTCATT
    GTGAGTGTGG GTCCGCCCAT CGAAAGCAGC GCTCAGGAGA ACTCAGGAGC GACCTCTACA
    GGAGACAAGA AGAAAAGCGC TCAGTCCACG GTGTGTCGAG AGAGGAACGC TGAGCTCGCC
    CGGTCTGACC CGGTGCGTCC GTTCATCAGT GGTCACGTAG CCAACAGTAT GGCAGCAGAG
    GTCATCGCTC TGCTGCACAG CCTCCTGACG GCCCCCGAGT CCAACACTGC TCAGATCTGG
    ACAAGCACTG CAGAAAAGGT GTTGTCCAGA GCTCTGATGT TCATCCCTCA GCTGGGGAAG
    TTTGCTGAGA GTATTCTGGA GAGCGGAAAC AGCAGCGGCA GGAAGTTGTC CAAGCTGCAG
    GCCATTGGTC GACAGGCGGT GGCGGCTCTC TGTGCGCTCG GCGGATTCAA GGAGACCATC
    AAGATTGGAT CTGAAGTGCA GGTGGTTGGT AAAGGTGTGT TGGACAGTGT CGGTGTGGTT
    ATATCCATTA ATGAGCAGGA GGGCATCGCC ACCGTCAAGT TTCCATCCTG CGAGTATCGG
    CGCTCCAGCA AAGCATCTGA TATCCTGACG GTGCCCATAT CACGCCTGTG CACGCCGCGC
    TCAGAGGCTC TGCCGCTGCA CAAGCTTTCC ATCACAGAGA AGGTTGTTCA GGCCGTTCAG
    TCCATGTTGC TGCCGCAGGA GGGCAGTCTG TCCATACACA CGTCTCTGCC GGCGTCTGGA
    GACGGCTCCA GTCCTGTGAT GGCAGTGGTG CGTCTGCTGG CCGAGATCAG AACCAGAGCG
    TGTCTGGTGA TGGCGCAGCT GCTGGAGGAC AGCTCTTTCT GTGAGGAGTT TATCCAGCAG
    TGTCCTGCAG CCGTGGAGGT GCTCAACCTC ATCGCTCAAG AGTGCAGTCC TGGAGAGCGT
    TTGGTGATGG TGGAGGCTCA GTGTGAGCGT CTGCGCATGC TTTATCGAGA CTGTGCTCGT
    CCTCCACCAC CGCCTGTGCA AACTGACCAA CGGCAGCCGA AGGAGATCAC GTGGTGCCCG
    TCTCGAGTCT TCCCTCCGGT TCGGGCCTGT ATGTTTTCCT CGCACTTGAC GTCGGTGACG
    TTTCTGGCTG ATCCGAGTGC AGGCGGAGGC CTTCCCAGAG GAACCTTCAT CTACGCCACT
    TCACCCGTGC CCATTCAGGC TCCGTCTTTT TACTGGGAGA TCGAGATCGT TTCGTTCGGA
    GACTCGGAGG ACGACAGCGG TCCAATAGTG TCCTTTGGAT TTTCCCCGGA GGCGGAGAAG
    AGAGACGGAG CCTGGACCAA TCCCGTCGGG ACCTGCCTGT TTCACAATAA TGGCCGGGCG
    GTTCACTACA ACGGCTCCAG TTTACTGCAG TGGAAAAGTG TGAGGCTCGA CGTGGCTCTG
    CTTCCTGGTG ATGTAGCCGG CATCGGCTGG GAGCGTCTGG AAGGCACTCC TCCTCCACCC
    GGTCAGCCTC CCAAAGGAAG AGTGTACTTC ACCTACTGCG GTCAGCGACT CGCTCCCTAC
    CTAGAGGATG TGGCTGGAGG CATGTGGCCC GTCGTTCACA TCCAGAAGAA GAATACGAAG
    ATTCACGCCA ACTTCGGCTG CCGGTTGTTT GCGTACGCTG AAGGCCAGGC TCACAGAAAC
    GCTGCTGATC TCTGCATGGA TCTGTCAGAG GAGATCAGTG CTAACTTCGA GGCTCTGCCC
    TTCGCCATGG CCTCCGACAG CGATAATGAT GCGGGCACCA GTGTGGCGTC TGACTCCGGC
    TCCCACGGGC CTCCTTGTCG CATCGCTGCC GTCGTCACAG CACAACAGCA ATACAACAGC
    GATGCTTCTT GCCACTACAA GGTAGAGCTG AGCTATGAGA ATCTGGTGGT GTCGGGACCG
    AACGCTCATC CGCCACCCAT CGCTGATGAC GAGAGCGATG ATGAGGATGA TGAAGACATT
    CCCCGGGAGG ATCATTACGC CCTGCTAGTG AAAGCGTGGG AAACTAAAGT GTTTCCCACC
    ATTCGCCGGC GGTTCAGGAA CGAGGCGGAG AGGAAATCTG GCCTGGATCA GATCAAGGGA
    GCGCTGCAGC TCGGTATGGT GGACATCGCT CGTCAGACGG TTGAGTTTCT GTATGAAGAG
    AATGGCGGGA TCCCTCGAGA CCTCTACCTT CCAACCATCG AAGACATTAA GGATGAAGCA
    AACAAGTTCA CCATCGACAA AGTCCGCAAA GGCTTGACGG TGGGCATCCG CTGTCCAGAG
    GGAAACAACA CCTGCAGCAG CACAGGAGGA ACCGCTCTAC CAAAGTTTGC CATCCGCGGC
    ATGTTGAAGA CCTTCAGCTT ACATGGAGTT GTGCTCGATG TGGATTCGGG TAATGAGCTG
    GTGCAGGTGG AGACGTATCT GCGCAGTGAG GGTGTGTTGG TGCGCTACTG GTACCCCATA
    GAGATGCTGG AGAGACCGCC GGCAGGATCC CGCAGGGCTG CAGCCAATGG CCTGGTGTCT
    TTGGACAGCA GCAACATCCA GATTCATCGG GAGCTGCTGC GCTGTGAAAG TGCTCTGGCG
    CGTCTGTACT GCCGCATGGC GCTGCTCAAC ATCTTTGCGC CCAAGAGTCC TCACACATTC
    ACGCGCCTCT TCCACATCCC TGCAGTGCGT GACATCACAC TCGAACACCT GCAGCTCCTG
    TCCAATCAGC TGCTGGCTCC GCCTCTGCCA GACGGCCGGA TCAGCTGTAA CTCTGTGCTG
    CTGGCTCAGT CGGTCCTACA TGAACTGCAG GGTCAGAGCT GCTCTCCCAC TGAGCTCTTC
    TATCAGGGAA ACGCTCAGAG TGTCCGCGAG TGGCTGAGCG TGGCCATCAG CAGAGCTCTG
    CATCAGGGGG AGGACAGTCT GCTGGAGCTC ACCAAACAGA TCTGCTGCTT CCTGCAGAGT
    GCGCCTGAGC AGTTCACGCT GGAGGAGATT CCCATCACCG AGTCAAAGGT CAGCATGGAC
    GTCAACTTCC CCGGCGCTGC GTTTGTGCTC GTCTCCTGCA AAGAGAGTCA ACTCAGCTGC
    CGCAAAGATT CCTCTCTGTA CAAAGCTCCA TGGGCTCGAG TGATGGTGTA TGGGCTCGGT
    CATAAAGTGC GGCGTAACGG CCAGCTGAAC CTGATGGAGG CCGTGTGTTT CCCGCTGGAC
    GCCTCTCCGT CCAACACGGG CCTCACTCCT CCACCCACAG CCAACCAGTA TCCCAGCATC
    ATCATCCCCA CCGACAGAGT GCACGTCAAA CTGGGTGTGT CTCCCCCTCC TGGAGCAGTG
    CTGGTGTTGC ACTCGTTGCC TCTTGAGTTT CCATTGGCGA TGGCCTTCGC TGAACAGCTG
    CTAACCTACA GTGTAGGAGA GACCGTTGAG CGCTCTGAGG ATGAGCTGGA CACGGTGCCC
    ACCTCTGTGC TCATGCAGGT CGTGGAGCTG CTAGGTGGCC TTTTGTGGAC CACAGATCTT
    GCTCCGAGCA TCAAGGAGCT GACCTTCCAC CTGCTTGCAG AGCTCCTCCG CAAGATCCAC
    GCTTTTGAGC GCCGCAAATC CCCTTCGGGT CTCTCTAGCC ACATCACGCT GCTTCTCAAC
    CCCTGCCTAA CAGTCCTGAT GGTTCTTCAA ACCGAACTAC GAAAGCTTTA CGACCACGAG
    ACCCAAAGCT GGGGGTTGGT TAGCGGAGGA TCCTGCACTT CCGCAACAGG CGGCAGTGGA
    GCGCTTGCAC TTTCTGCAGA GCGGAGCCGC TTCTCTACAT ACTTCCACGC ACTGATGGAG
    GTTTGCCTGG CAGTGGCGGA AGTCACTTTA CCGCTGAGTT CTGGTTCTGG GACGTCAGTA
    ATCGGCGTCC CTTCATCAAC AAGCACAGCC AACTGCAGTG ATTCCTCATC CTCTTCTTCG
    TCCTCTCCAG GCCAGACGCC GCAAAGTCCG AGTTTGCTTT CAAAGCGCAA AAAGGTGAAG
    CTAAAACGTG AGCGCGCTGC AGTCGGCTCA GCTTCCGCCG TCTCAGGGAA AAGTGGCTCG
    AGATCATCAG AATGCGACAA TGCTCTATTA AACATGAAGC CTGAAGACAT GTTGTGGTTT
    CACCGGGGGT TGACCTTGCT TTTGATTTTA CGGCATCTTA CTAATAAGGA CCCGCAAGGT
    CTTGGCGTCA CCGGCGACGC TGTGGCAGAC GCTTGCCAAG CTTTGGTTGG CTCGACAGCT
    CACAGTCGCT TGCTGGTGCT CTCAGGAATC CCGACTCACC TGGAGGAGTC TGTGGTGCGC
    AATGCAATAC GAAGAGCATG CAATGCTCAT GGCGGGCTCT TCAGGGATGA GATCTTCATT
    CCAGTTCAGG AAGAAGACCC TAAAAAAGCC AAAGCGGAAA CGACATGTGT TCCTCAAGAT
    GTAAAGAGAG AATGCACTGC AGGAAGCCCC AACAGCAACA GTGCGGCAAT GAGTGTCTCC
    AGCGCTTTAC AGGCAGCCTT CAGAGCCTCC GGAGAGCCCT TGTCCAATCA GGAGATGGTA
    AACAGTCCTT CCCAGCAACA AGCACCTCTG GATCCACTCA CAGTGTCCAG TCAGGAGAGT
    CTGGACGTGT CTCTGTGTAG CACAGAAAGT CAAGGGAGTC TCGGCAGCCT CGGGGAGCAG
    GATAGTGCTG CCACGTCAGT CTCAGATGGG AGCTCCATGT ACACGGTGGC CTCACCTGAT
    GTTCATGCCA GTGTGACGCG TCCCATAAAA GGCTATGCTG TCATTGAGGT AAGGGCACGA
    GCTCAGGTGG AGAAGATTCG GGCCAGTCTT TTCAACAGCA GCGATCTGAT TGGCCTCTCC
    AATCTGGAAA GGGAGGAGGA GCTAATGGAG ATGACCAATG AGGAGATCCT CACCGCCTCC
    AGTGTCAATC AGAGTTTATT TGACACACAG GGCAGCTCTG CTCTGGAGGA GTACTTCCTG
    GATAAATCCA TCAGAGGAGA CAAGCTGGTT CCTGGAGCTC GTGACGTGCT GACAGACATC
    TTTAAGAGCT GCATATACGC AGAGCAGGCG CTGAGCCCCG TTCCCGTGAA ACCTGTCAAA
    GTCTCAGACA TTTACCTCAG CAAAGAGCAG ATCAACTCTC AGTCTCCCGG AAACCTGCTA
    CACGTGTTTT TCACACACGT TCGGCCTCCC AAAAAAGTGC TCGATGACCA GCTCACACAG
    ATTCTGCGGA AATACGGCAC AGTAAAGCCC AATAAAAACA AGCACAGCAA AGCGGGCAAA
    GAGCAGCACC AAGGCAAAGT GGTGAGCACA AAAAGACCCA TCACCAAACC TCCGGCCAAA
    GAGAAGTCCA TGCTGAACAG CGTGCGAACT GTACTGAGTG AGAAAAAAAC CGTCCTCAAG
    CCAAAATCTC CAGAGAAGAC CAAACCCGAT GAAAAAGACC CCGAAAAATC TCCTGCCAAA
    AAACCCGAAG TGCCAGAGGA GAAGTTTTTG ACGCTGGAGG GTTTCCACAA GTTTACTCTG
    GACAGAGCCA AGCAGGATAT CCGCAGTGTT TGGCGGGCGA TCCTGGCCTG TGGATATGAC
    CTGCACTTTG ACAGATGCAC ATGCATTGAT TCCCGACATG CTCAGAAAGC CAGCAGAAAA
    TGGAGCTTCG AGATGGACTT TGCGTTGGTG CAGTTTGTGA ACCGTCTCTG TCGTCACCTG
    GCCATCACTC CTGCCCGCCT GCATCCACAT GAGGTTTACC TGGACGCCAG CAAAGATGGC
    GCGGATCCTG AGGTCGCGTG TCTGTTAGGT GTCCCAGTGG AGAGTTTGCG TCTGCGCTTT
    GCTCTGCTGC AGTCCCTCAA CAGCACGCTG GAGAGCTTCT TCCTGCCTTT AGTGGAGCTG
    AGACTCACAC AGACCTACCA GAACAGCATC GCCACACTTC TGTGCGAGGC CAAAGGCCTG
    ATATTTTACG ACACTAAGGT GACGGTGATG AACAGGGTGT TGAACGCTAC CGTGCAGCGG
    ACGGCAGATC ACGCAGCGCC TGAAATCACT CTCGACCCGC TGGAGATTGT TGGAGGAGAG
    ATGCGCGCTG CGGAGAACAC TTATTTCTGC CAGGCAGCGC GGCAGCTGTC GTGTGTGCCA
    TCGTCTCAGC TGTGTGTGCG CATCGCCAGC GGAGGAGACC CCACATACGC CTTCAACATC
    CGCTTCACCG GAGAGGAGGT CCACGGCACC AGTGGCTCTT TCAGGCATTT CCTATGGCAG
    GTGTGTAAGG AGCTCCAGAG TTCAGCTCTC TCTCTTCTTC TGCCCTGCCC GAGCGCCGCC
    GCTAACCGCA ATAAGGGGAA GTACATCCTG ACTCCGTGTC CCATCACATA CGCTGAGGAG
    CAGCTGCTGA ACTTCTTCGG GCAGCTTCTG GGCATCGCCA TCCGGGCCGA TGTGCCGCTG
    CCTCTGGACC TGCTGGGATC CTTCTGGAAG GGTCTGGTCG GAGAGGCTCT CGATCCCGAA
    GCTGACCTGC GGGAAGCAGA TCTGCTCACG TACAGCTACA TCAAGAAGTT TGAGAGTTTG
    TGTGATGAAG CGGAGCTGGA AGCGCTGTGT GCTGAAGTGT CGTCTCAGAG TCCCCTGGGG
    GAGAGTCCAG ACTCCCCCAG CCGGCCCTGC TGCACCTTCA CATACATCAC TATGACTGGA
    GAGGAAGTGG AGCTGTGCCA GGGAGGACGT GACATCGCTG TCAGCTGGGA GAATAAGGAT
    GTGTATGTGC GAGCGGTGCG TGAGCTGCGT CTGCGGGAGC TGCAGAATGT GGAGTGTATG
    TCTGCGGTGC GCGCCGGTCT GGGCTCCATC ATCCCACTGC AGCTGCTGAC TTTACTGAGT
    CCCGCAGAGG TGGAGCTGCG CACCTGCGGT CTGCCGGACA TCAACCTGGA GTTCCTGAAG
    GCGCACACCA TGTATCAGGT GGGTCTGATG GAGACGGATC AGCATATCGA GTTCTTCTGG
    GCGGCGCTGG AGATGTTCAC GCAGGAGGAG CTCAGCAAGT TTATTAAGTT CGCCTGCAAC
    CAGGAGAGGA TTCCCTTCAC CTGCCCGTGC AAAGATGGGG GTCCGGACAC CGCACATGTG
    CCCCCCTATC CCATGAAGAT CGCCCCGCCG GACGGCACCG TGGGCTCTCC AGATCAGCGC
    TATATCCGCG TGGAGACGTG TATGTTCATG GTCAAGCTGC CGCAGTATTC CTCCCTGGAT
    GTGATGCTGG AGAAACTGCG ATACGCTATT CACTATCGAG AAGATCCTCT GAGCGGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301943.1
    CDS430..13689
    Translation

    Target ORF information:

    RefSeq Version XM_009303668.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio HECT domain E3 ubiquitin protein ligase 4 (hectd4), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303668.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGCTCAG GTGCTGTGGA TTCTTCCCAG TGGCTGTCAG TGAAGGAAGA GACTATTTTC 
    CTTCACGATG GACTAATTCG GGTCACGGAC CTGGCTGAGC TGCCCAGCGA AATCGGCGTG
    TCTGAACAAG CTGAATCCGA GCAGGAGATC TTGACGTTTG AGACCAAGAA TCCAGTGGAG
    CTAGCCGAGC GTCTCCGAGC CGTCTGTGGA AACCAGAGCA ACGCTTATGC ACGCCTTTTG
    GAGTATCGTC TGAATGCACT GCGTGGCCTG TGGGGGGCGC AGCGTCAGCT GGCCCTGGAG
    GAGCAGCAGG ATCATGACGG GGTTGTGTCA GCCAGCGGCG GAGGGTCCGA CGAGGAGACA
    CTGGCCCTAC TCAAACGACA GGGTTTACTG CAACCCCAAC ACCACCACCA TCCACACCAC
    CCTCCGATGG CCAGCGGCTC AGAGCAGGCA CCATTCACCT CCCGTGTGGG CCTGCTGTTG
    GTTTTCCCAC TGCTGCAGTC GCAAACTCGG CACGACCCTG CGCTGTGCGG CGTCACAGCG
    GAGGTGTTGC TCGCATGTCT GCGGGACTGC CAGCCACTCA GCCTGGGGAA GGAGCCCGCC
    GACTGTCTTA ATGGCCTGGA GAGGTTGCTG TGCTCCTGGT TGGAGGAGAC GGAGGGTTCG
    GCACCAATGA GGCCACTGCT CACCCAACAG CAGAGACAGA ACGCAGCTGC GGCGCTAGTG
    GCTCTGGCAT GTGCAAGGGG CTCCCTCAAA ACCTTCATCC ACACGGTCCA CCTGCTGCAG
    AAACAAACAG ACCTGGGTCA GCTTCCTGTG GCTGATGTTC TCTATCGACT GCTGCTTCTT
    GAAGGAGGTC CAGGTTCGCC CTCATGTCTG CTGGGGGGGA AACATATTGT GTCGTGGGGC
    TTTGAGGACA TGCTGCCCAC ACCAGAGAGC AGCGCCGCAG GAGCCGACAG TAAAGACACA
    GATCTGGGCC GCAGCCTGGC CACAGATGGA TTCTATCTCT ACACCACGAG CTCTTTTGGG
    AAGGGTTTGA GCAAGCTGGG CTCCGGGCAG CACGGGACGC TGAGGGGTTT TGTGTACTGT
    CGTAATGAGG AGATGGAGGC GGGCTGGGTG GCTCACAGCA GTGGATTTCT GCTCCACCGT
    CCTGCATCAT TTGACACTAA ACCTCATCAG CTGTGCCACG TCATCGACCC GTTCACACTG
    CAGGTTTCTC AGGTGTTAGC GATGCCCGTT CATCATTTCC CAGTGGGCAG CTGCCTGACC
    ACGCTGCGTC TGTGTTCTGA CGGCACATAC TTGTATTGGG TTTGGTCTCC GGTCAGCATC
    AACGAGAAAA CGCAGAAAGG CCACTCTGTC TTCATGGACG TCTTCCAGTT AACGAGTGAG
    TCCGGTGTGT GTGTGGCCAG TCCTGTACAA GACCGGGTGA TCCTGTTGCG AAAGGAAGGC
    GAGTCGTCCA AGTGCTTGAA TGAGTTGCTC CTCAGCCGAA TGTCGCGCTT CCGAGCCTCG
    CACTCGTCCA CACTGGCGGC TCTCACCGGC TCCGCCATCA CCAACCCTGT CAAAGAGGAG
    CAATCGGTGG TCAACACAAG CTGTGGTCTG TCCATCAAAA CACTGAGGAA GACACCCATG
    TATGTGTGCG GGACGTCCCT GGTGATGCTG GTGCCGACTC CAGGTGTTGC TGGGAGCTCC
    GCCACGCGCT CGCTGTTCGG GGGATCCAGT GGCCTTTCTT CTCTCAAGAT CTTGGCCTCT
    AGTTTGGTGT TTAACCTGGC GGACGGTCAG TTCAGCAATC GCGTGGATCT GGTCGACGCT
    CCGGGAAGTT CTCTGGGTCG TGGAGCTCAG GTTGCAGGAC TCGGCGCCTG TTATGATGCA
    CTGAACAACT TAATCTGGAC GTGTTCGAGC GACTACATGG ACCAGTGGTG CAACCCTGGA
    AATCAGGCCT TCCATCATGT GTGCCAGCGA CTCGGCGTCA GTCACGCCAT CAGCCAGACC
    AAAGAAGAGA TGATCGACAC ATCCAGCGTG ATCAGCCAGC TGCTCCATCA TGTGGGAGCC
    ATGTGTATCC ATCAGCTGAA CCTGCTGGCC GCCAGCAGTA GTTTACACTT GGGCACCTTC
    ATAGGGAAGC AGCCAATGGA GGCCTGTCAC TTCAGCTCCA TCTGTGACGT GATGGAGAAA
    GCCATGGTCA ACGGAGACAC CCGCATCATC CGCTGCATCC TGGTCGTTTT TCAGGTGGTT
    TTCCAGTTTT TTAATCCAAA CTCAGAGCAG AACCGGGAGT GTGTGAAGCG CTCAGGACTG
    CTGCTGTGGC AGTTACTCAT GGCTCCACCT GATCAGATCC AGCCGGAAAT ACAGAAAGAA
    GTTTGTCTCG CCATCAGCTC AGGTTTAAAC ACCCTGTATC AGAGCGAATC TGAGTTGAAC
    AACCTGCTGA AGTTTGTTTT CACAGAGGGG GAGAAGAACA GTGGTTTGTC TCAGTTACGA
    GACGTGATTT TAAACAATCT GGCTAACCAG TTACAGAAGA ACCGCTTTGG TAGTGAAGAT
    GATGATCACT ACAGGCTGAA GGATGAGCTG CTGCAGTGCA TCTTGAAGAC GGTGGTGCGC
    GAGTCGTGTC TCCTCATCAC CAAATGTCAA ACCGTTTCCA AAGAAGACCT TCAGAAGCTG
    CTCTCTACTG TACCTGTGGT GTCTCCCAGC TTGCGTTATT TAATGGCTGT GCAGAATCAT
    CTGCTCAGCA ACACCATCCT GCTGCACCCG GAGGACATGG AGGACAGCGA CAGCTCCCTG
    CAGGGGGAAA CAATGAAGGT ACAGGAGCTG CAGACGAGCA TCTTGTCTCT TGCGTTGAAG
    ATTCTGGTTG GATGTGATGA AGTTCTGGAG ACGCTTCAGC AGGTGACCAG CTCTCTCATC
    AACAGCAGCA TCAGCGACCG AGAGACCAGG CTCAGAGGTC TTGAACAGGT GACCAAGGCC
    ACCATGTTGG GTCATTTGCT GCCGGTTCTG CTGACGTCAC TGATGCACCC GAACCTCCAA
    ACACTCACGC TAGCAGATGC GCTCATGCCA CAGCTAGTCC AGCTCGTCCT CTACACTAGC
    CAGACCGCTT TGCTGCTAAA GACACATTCT CCATTATTTG TTGAGGATTC CCCAATAAAT
    CAAGGCTCCA GCCAGCCCTC ATTAAATGAC AGTTTTCAGA TTCTGGAGGA GAGAGAGGAG
    CCTGGCTTTC TGACGGGGCT GAAGATCCCC GCCCCGTGGG CTGCTGGGAA AACGGTGGAA
    ACTGTTCACC CGGTGCGGGA TAACTATAAA TTTAAAGACA CAGTGCACAT TCCTGGAGCT
    CGCTGTCTGT ACCTGCGCTT CGACCCGCGC TGCTCCTCAC AGTACGACTA CGACAAGCTT
    GTGATTTACG CCGGGCCGAA CACAAACAGT CGTAAAGTAA GCGAGTATGG AGGAAACACC
    ATGGGTTATG GCAGCCGCAG TGTATTGGGA ACCGGATGGC CAAAAGATCT GGTCAAGGTG
    GAAGGAGATA CGGTGACGTT CTCTTTTGAG ATGAGAAGTG GACGCGAGCA CAACACTCCA
    GATAAAGCCA TGTGGGGTTT CTCGTGCACC GTCAGAGCGC AGGAGTCGTC TGAGGATGTG
    TCTGGCGGTC TGCCGTTTCT GGCTGATCTG GCTCTGGGTT TATCAGTGTT AGCCTGCGCT
    ATGCTACGAA TCCTCTATAA TGGGCCTGAA ATCACACGAG AGGAAGAAGC TTGTCACGAC
    CTTCTCTGCT CCAAATTACT GCAGAGGTGT CAGTGGCAGG TGGAGGCGAA CGGAGCCATC
    TCTCCTGCAC TCACGCCATC TCCGTCTCCT CTTCCTCTGA CTATCGATGA GGAGCGCGAG
    TTCACATATC CATCAGATAT CCTCGCTCCG CCGCCGGGGC CCATCCCGAG CGCCTGCTGT
    GAGCTGCCCC GCATTCACTT ACCGCAGGGT ATCATGGGCC GCCTGAGGGA GCTGTCTGGA
    CGTGCTCGGC CACAGTTTCG CCCCAGCATC AAAGAGGTGA TCCGTCCAGA CGTGATGGAG
    GAAGTGATTG TGTCGTGTGT GATTAAACAC TTGGGTCTGG TGGACGCGCT GCAGTCGCTG
    GTCAACTTCC AGTACAGAGA ATCTCACAGA GAAGAACACA ACCTGCTCTG CAAGATCATG
    GCTGAGACCT TCAAGAAGAT CAACGCCATG GAGAGACAGT TACAGAGCGT GGCTGAGCTG
    GAGCAGAAGT GGCAGAATGA AGTAGATGAA GCTCAGCAGG GAAAACTGGA GAACAACACT
    CCTTTCTTCC ACGATTATCA CTTCTTTGAG AATAAAATGA AGGAGCTGGA GCTTCTCTGT
    TCGCTAAAGG AGGTTCCTCT GGACTGCACT GATCCAGAAA ACGCTGTGAT GGCTTTGAGA
    GAGAAGTTTT TTCATGAGGT GAACTCCACG CTGCAGCAGC AGAGAAGTGT TGTTCCTCTG
    GCTAAAACCA AAGCGCTTGT GAAGAGTTTG ATCAACCGCT CGGAGCTGCT GCTTCATGTC
    ACCATCGCTC CGCAGTGCAA GAGTCTGTCA TCAACACCCA CAAGCACACC GGCCTGTAAG
    TCGGTGTCAG ACGCTAAATC GATGGCTCCC GTGGTGAAGC AGCCGGTGTT CCTGCGCAGC
    ATGTCTGCTC CGTCAGATCT GGAAATGATC GCCAGCAGTG ATGTGGAGTT CACACACGGA
    GCGCAAAGGA GGCGGCAGCA CCCCTCCAGC CACCGGAGCA GCTCATTTAC ACTCCTGCAG
    TCATTGGTGA TTGAAGACAG TAAAGATAAA CCCACGTACA GTGTGCTGCT GGGACAACTG
    TTCGCCTTTA TAGGAACCAC TCCTGACCAA ACCGTGTCGA GCAGCAGTTT TCTGTCCGCA
    GCTCAGACGC GCTGGAGAAG AGGACGAACA CGCAAACAGG CTCTGGTTCA CATGCGGGAG
    CTGCTGACCG CTGCCGTCCG CGTCGGTGGA GTCACTCATC TGGTCGGACC CGTCACTATG
    GTCCTGCAGG GGGGACCCAG GGTGGAGGAG CTCACCTGTG GTGGGATGAT GGAGCAGGTG
    CAGGAGGCGT TTGGAGAGAC CATGACGTCA GTGGTGTCTC TGTGTGCCCG ATACCCCATC
    GCCTGTGCCA ACAGCATCGG CCTGCTCTGC ACCATCCCCT ACACCAGGAG TGAGGAGCAG
    TGTCTGGTGC GCAGCGGTCT GGTTCAGCTC ATGGATCGCC TCTGCAGTCT GAGCAGTCAG
    AGAGACTGCA GCTCCAACGA GAAGCAAACC AAAAAGCAGA AAGTGGCCAC CATGGCCTGG
    GCTGCGTTTC AGGTGCTGGC CAACCGCTGC ATTGAGTGGG AGAAAGTCGA AGGAGGCTCC
    ACAGACGCAG TGCATTCAGG GTTGGCTCGA CAGGTGTCCA GCCTTCTGAC CAATCATCTG
    GCTCGAGCGA CAGACTGCTG TGGAAACCAG GCGGCAGGAA ATGATGCGCT GCAGGATGTG
    CTCAGTTTAC TCAACGATCT GTCCAGGAGT CACATAGGCA AGACCATCCT GAGCCAGCCT
    GCCTGTGTGT CCAAGCTGCT GTCTCTGCTT CTGGACCAGC GACCGTCGCC CAAACTGGTG
    CTGATCATCC TGCAGCTGTG CCGCGCCGCG CTGCCCCTCA TGAGTGTGGA GGATTGTGGA
    AATGTAGCGC TGCCGTCCTG GAGCTACTCC ACAAACGCAC TGGACGCCGA GCAGCAGGAC
    GCCAGCGACC CTGCCTTCCG CATCGCATCT CTGCTGCTCG CCAAACTGGC CGACTATGTG
    GTGCCAGGCT GTCAGACGTT GCTCTCTCCC AGCGCCTCTG AGCCGGACAC CAGTCTGAGC
    CGAGCCTGTC CCAAGAGCAG CTCTAAGACC GACAGAGATG CCAGCGAGGA GGGCGAAGCG
    GTGGACGGAA AACTCTCCAT ATTCATCCAT AAGAGAGAAG ACCAGTCGTC GCACGAGGTG
    CTGCAGCCTC TGCTCAGCAG CTCAGAGGGT CGACCGTTTC GTCTCGGCAC TGGAGCCAAC
    ATGGAGAAGG TGGTGAAGAT GGATCGAGAC ATGACCAAGA GCGGCTGCTG TGAGGTCATC
    ACTGAAGAGG CGTCCGCTGC TCTCAGGAAA GCCAATAAAT GGGCTCAGTC GGGGCTCATT
    GTGAGTGTGG GTCCGCCCAT CGAAAGCAGC GCTCAGGAGA ACTCAGGAGC GACCTCTACA
    GGAGACAAGA AGAAAAGCGC TCAGTCCACG GTGTGTCGAG AGAGGAACGC TGAGCTCGCC
    CGGTCTGACC CGGTGCGTCC GTTCATCAGT GGTCACGTAG CCAACAGTAT GGCAGCAGAG
    GTCATCGCTC TGCTGCACAG CCTCCTGACG GCCCCCGAGT CCAACACTGC TCAGATCTGG
    ACAAGCACTG CAGAAAAGGT GTTGTCCAGA GCTCTGATGT TCATCCCTCA GCTGGGGAAG
    TTTGCTGAGA GTATTCTGGA GAGCGGAAAC AGCAGCGGCA GGAAGTTGTC CAAGCTGCAG
    GCCATTGGTC GACAGGCGGT GGCGGCTCTC TGTGCGCTCG GCGGATTCAA GGAGACCATC
    AAGATTGGAT CTGAAGTGCA GGTGGTTGGT AAAGGTGTGT TGGACAGTGT CGGTGTGGTT
    ATATCCATTA ATGAGCAGGA GGGCATCGCC ACCGTCAAGT TTCCATCCTG CGAGTATCGG
    CGCTCCAGCA AAGCATCTGA TATCCTGACG GTGCCCATAT CACGCCTGTG CACGCCGCGC
    TCAGAGGCTC TGCCGCTGCA CAAGCTTTCC ATCACAGAGA AGGTTGTTCA GGCCGTTCAG
    TCCATGTTGC TGCCGCAGGA GGGCAGTCTG TCCATACACA CGTCTCTGCC GGCGTCTGGA
    GACGGCTCCA GTCCTGTGAT GGCAGTGGTG CGTCTGCTGG CCGAGATCAG AACCAGAGCG
    TGTCTGGTGA TGGCGCAGCT GCTGGAGGAC AGCTCTTTCT GTGAGGAGTT TATCCAGCAG
    TGTCCTGCAG CCGTGGAGGT GCTCAACCTC ATCGCTCAAG AGTGCAGTCC TGGAGAGCGT
    TTGGTGATGG TGGAGGCTCA GTGTGAGCGT CTGCGCATGC TTTATCGAGA CTGTGCTCGT
    CCTCCACCAC CGCCTGTGCA AACTGACCAA CGGCAGCCGA AGGAGATCAC GTGGTGCCCG
    TCTCGAGTCT TCCCTCCGGT TCGGGCCTGT ATGTTTTCCT CGCACTTGAC GTCGGTGACG
    TTTCTGGCTG ATCCGAGTGC AGGCGGAGGC CTTCCCAGAG GAACCTTCAT CTACGCCACT
    TCACCCGTGC CCATTCAGGC TCCGTCTTTT TACTGGGAGA TCGAGATCGT TTCGTTCGGA
    GACTCGGAGG ACGACAGCGG TCCAATAGTG TCCTTTGGAT TTTCCCCGGA GGCGGAGAAG
    AGAGACGGAG CCTGGACCAA TCCCGTCGGG ACCTGCCTGT TTCACAATAA TGGCCGGGCG
    GTTCACTACA ACGGCTCCAG TTTACTGCAG TGGAAAAGTG TGAGGCTCGA CGTGGCTCTG
    CTTCCTGGTG ATGTAGCCGG CATCGGCTGG GAGCGTCTGG AAGGCACTCC TCCTCCACCC
    GGTCAGCCTC CCAAAGGAAG AGTGTACTTC ACCTACTGCG GTCAGCGACT CGCTCCCTAC
    CTAGAGGATG TGGCTGGAGG CATGTGGCCC GTCGTTCACA TCCAGAAGAA GAATACGAAG
    ATTCACGCCA ACTTCGGCTG CCGGTTGTTT GCGTACGCTG AAGGCCAGGC TCACAGAAAC
    GCTGCTGATC TCTGCATGGA TCTGTCAGAG GAGATCAGTG CTAACTTCGA GGCTCTGCCC
    TTCGCCATGG CCTCCGACAG CGATAATGAT GCGGGCACCA GTGTGGCGTC TGACTCCGGC
    TCCCACGGGC CTCCTTGTCG CATCGCTGCC GTCGTCACAG CACAACAGCA ATACAACAGC
    GATGCTTCTT GCCACTACAA GGTAGAGCTG AGCTATGAGA ATCTGGTGGT GTCGGGACCG
    AACGCTCATC CGCCACCCAT CGCTGATGAC GAGAGCGATG ATGAGGATGA TGAAGACATT
    CCCCGGGAGG ATCATTACGC CCTGCTAGTG AAAGCGTGGG AAACTAAAGT GTTTCCCACC
    ATTCGCCGGC GGTTCAGGAA CGAGGCGGAG AGGAAATCTG GCCTGGATCA GATCAAGGGA
    GCGCTGCAGC TCGGTATGGT GGACATCGCT CGTCAGACGG TTGAGTTTCT GTATGAAGAG
    AATGGCGGGA TCCCTCGAGA CCTCTACCTT CCAACCATCG AAGACATTAA GGATGAAGCA
    AACAAGTTCA CCATCGACAA AGTCCGCAAA GGCTTGACGG TGGGCATCCG CTGTCCAGAG
    GGAAACAACA CCTGCAGCAG CACAGGAGGA ACCGCTCTAC CAAAGTTTGC CATCCGCGGC
    ATGTTGAAGA CCTTCAGCTT ACATGGAGTT GTGCTCGATG TGGATTCGGG TAATGAGCTG
    GTGCAGGTGG AGACGTATCT GCGCAGTGAG GGTGTGTTGG TGCGCTACTG GTACCCCATA
    GAGATGCTGG AGAGACCGCC GGCAGGATCC CGCAGGGCTG CAGCCAATGG CCTGGTGTCT
    TTGGACAGCA GCAACATCCA GATTCATCGG GAGCTGCTGC GCTGTGAAAG TGCTCTGGCG
    CGTCTGTACT GCCGCATGGC GCTGCTCAAC ATCTTTGCGC CCAAGAGTCC TCACACATTC
    ACGCGCCTCT TCCACATCCC TGCAGTGCGT GACATCACAC TCGAACACCT GCAGCTCCTG
    TCCAATCAGC TGCTGGCTCC GCCTCTGCCA GACGGCCGGA TCAGCTGTAA CTCTGTGCTG
    CTGGCTCAGT CGGTCCTACA TGAACTGCAG GGTCAGAGCT GCTCTCCCAC TGAGCTCTTC
    TATCAGGGAA ACGCTCAGAG TGTCCGCGAG TGGCTGAGCG TGGCCATCAG CAGAGCTCTG
    CATCAGGGGG AGGACAGTCT GCTGGAGCTC ACCAAACAGA TCTGCTGCTT CCTGCAGAGT
    GCGCCTGAGC AGTTCACGCT GGAGGAGATT CCCATCACCG AGTCAAAGGT CAGCATGGAC
    GTCAACTTCC CCGGCGCTGC GTTTGTGCTC GTCTCCTGCA AAGAGAGTCA ACTCAGCTGC
    CGCAAAGATT CCTCTCTGTA CAAAGCTCCA TGGGCTCGAG TGATGGTGTA TGGGCTCGGT
    CATAAAGTGC GGCGTAACGG CCAGCTGAAC CTGATGGAGG CCGTGTGTTT CCCGCTGGAC
    GCCTCTCCGT CCAACACGGG CCTCACTCCT CCACCCACAG CCAACCAGTA TCCCAGCATC
    ATCATCCCCA CCGACAGAGT GCACGTCAAA CTGGGTGTGT CTCCCCCTCC TGGAGCAGTG
    CTGGTGTTGC ACTCGTTGCC TCTTGAGTTT CCATTGGCGA TGGCCTTCGC TGAACAGCTG
    CTAACCTACA GTGTAGGAGA GACCGTTGAG CGCTCTGAGG ATGAGCTGGA CACGGTGCCC
    ACCTCTGTGC TCATGCAGGT CGTGGAGCTG CTAGGTGGCC TTTTGTGGAC CACAGATCTT
    GCTCCGAGCA TCAAGGAGCT GACCTTCCAC CTGCTTGCAG AGCTCCTCCG CAAGATCCAC
    GCTTTTGAGC GCCGCAAATC CCCTTCGGGT CTCTCTAGCC ACATCACGCT GCTTCTCAAC
    CCCTGCCTAA CAGTCCTGAT GGTTCTTCAA ACCGAACTAC GAAAGCTTTA CGACCACGAG
    ACCCAAAGCT GGGGGTTGGT TAGCGGAGGA TCCTGCACTT CCGCAACAGG CGGCAGTGGA
    GCGCTTGCAC TTTCTGCAGA GCGGAGCCGC TTCTCTACAT ACTTCCACGC ACTGATGGAG
    GTTTGCCTGG CAGTGGCGGA AGTCACTTTA CCGCTGAGTT CTGGTTCTGG GACGTCAGTA
    ATCGGCGTCC CTTCATCAAC AAGCACAGCC AACTGCAGTG ATTCCTCATC CTCTTCTTCG
    TCCTCTCCAG GCCAGACGCC GCAAAGTCCG AGTTTGCTTT CAAAGCGCAA AAAGGTGAAG
    CTAAAACGTG AGCGCGCTGC AGTCGGCTCA GCTTCCGCCG TCTCAGGGAA AAGTGGCTCG
    AGATCATCAG AATGCGACAA TGCTCTATTA AACATGAAGC CTGAAGACAT GTTGTGGTTT
    CACCGGGGGT TGACCTTGCT TTTGATTTTA CGGCATCTTA CTAATAAGGA CCCGCAAGGT
    CTTGGCGTCA CCGGCGACGC TGTGGCAGAC GCTTGCCAAG CTTTGGTTGG CTCGACAGCT
    CACAGTCGCT TGCTGGTGCT CTCAGGAATC CCGACTCACC TGGAGGAGTC TGTGGTGCGC
    AATGCAATAC GAAGAGCATG CAATGCTCAT GGCGGGCTCT TCAGGGATGA GATCTTCATT
    CCAGTTCAGG AAGAAGACCC TAAAAAAGCC AAAGCGGAAA CGACATGTGT TCCTCAAGAT
    GTAAAGAGAG AATGCACTGC AGGAAGCCCC AACAGCAACA GTGCGGCAAT GAGTGTCTCC
    AGCGCTTTAC AGGCAGCCTT CAGAGCCTCC GGAGAGCCCT TGTCCAATCA GGAGATGGTA
    AACAGTCCTT CCCAGCAACA AGCACCTCTG GATCCACTCA CAGTGTCCAG TCAGGAGAGT
    CTGGACGTGT CTCTGTGTAG CACAGAAAGT CAAGGGAGTC TCGGCAGCCT CGGGGAGCAG
    GATAGTGCTG CCACGTCAGT CTCAGATGGG AGCTCCATGT ACACGGTGGC CTCACCTGAT
    GTTCATGCCA GTGTGACGCG TCCCATAAAA GGCTATGCTG TCATTGAGGT AAGGGCACGA
    GCTCAGGTGG AGAAGATTCG GGCCAGTCTT TTCAACAGCA GCGATCTGAT TGGCCTCTCC
    AATCTGGAAA GGGAGGAGGA GCTAATGGAG ATGACCAATG AGGAGATCCT CACCGCCTCC
    AGTGTCAATC AGAGTTTATT TGACACACAG GGCAGCTCTG CTCTGGAGGA GTACTTCCTG
    GATAAATCCA TCAGAGGAGA CAAGCTGGTT CCTGGAGCTC GTGACGTGCT GACAGACATC
    TTTAAGAGCT GCATATACGC AGAGCAGGCG CTGAGCCCCG TTCCCGTGAA ACCTGTCAAA
    GTCTCAGACA TTTACCTCAG CAAAGAGCAG ATCAACTCTC AGTCTCCCGG AAACCTGCTA
    CACGTGTTTT TCACACACGT TCGGCCTCCC AAAAAAGTGC TCGATGACCA GCTCACACAG
    ATTCTGCGGA AATACGGCAC AGTAAAGCCC AATAAAAACA AGCACAGCAA AGCGGGCAAA
    GAGCAGCACC AAGGCAAAGT GGTGAGCACA AAAAGACCCA TCACCAAACC TCCGGCCAAA
    GAGAAGTCCA TGCTGAACAG CGTGCGAACT GTACTGAGTG AGAAAAAAAC CGTCCTCAAG
    CCAAAATCTC CAGAGAAGAC CAAACCCGAT GAAAAAGACC CCGAAAAATC TCCTGCCAAA
    AAACCCGAAG TGCCAGAGGA GAAGTTTTTG ACGCTGGAGG GTTTCCACAA GTTTACTCTG
    GACAGAGCCA AGCAGGATAT CCGCAGTGTT TGGCGGGCGA TCCTGGCCTG TGGATATGAC
    CTGCACTTTG ACAGATGCAC ATGCATTGAT TCCCGACATG CTCAGAAAGC CAGCAGAAAA
    TGGAGCTTCG AGATGGACTT TGCGTTGGTG CAGTTTGTGA ACCGTCTCTG TCGTCACCTG
    GCCATCACTC CTGCCCGCCT GCATCCACAT GAGGTTTACC TGGACGCCAG CAAAGATGGC
    GCGGATCCTG AGGTCGCGTG TCTGTTAGGT GTCCCAGTGG AGAGTTTGCG TCTGCGCTTT
    GCTCTGCTGC AGTCCCTCAA CAGCACGCTG GAGAGCTTCT TCCTGCCTTT AGTGGAGCTG
    AGACTCACAC AGACCTACCA GAACAGCATC GCCACACTTC TGTGCGAGGC CAAAGGCCTG
    ATATTTTACG ACACTAAGGT GACGGTGATG AACAGGGTGT TGAACGCTAC CGTGCAGCGG
    ACGGCAGATC ACGCAGCGCC TGAAATCACT CTCGACCCGC TGGAGATTGT TGGAGGAGAG
    ATGCGCGCTG CGGAGAACAC TTATTTCTGC CAGGCAGCGC GGCAGCTGTC GTGTGTGCCA
    TCGTCTCAGC TGTGTGTGCG CATCGCCAGC GGAGGAGACC CCACATACGC CTTCAACATC
    CGCTTCACCG GAGAGGAGGT CCACGGCACC AGTGGCTCTT TCAGGCATTT CCTATGGCAG
    GTGTGTAAGG AGCTCCAGAG TTCAGCTCTC TCTCTTCTTC TGCCCTGCCC GAGCGCCGCC
    GCTAACCGCA ATAAGGGGAA GTACATCCTG ACTCCGTGTC CCATCACATA CGCTGAGGAG
    CAGCTGCTGA ACTTCTTCGG GCAGCTTCTG GGCATCGCCA TCCGGGCCGA TGTGCCGCTG
    CCTCTGGACC TGCTGGGATC CTTCTGGAAG GGTCTGGTCG GAGAGGCTCT CGATCCCGAA
    GCTGACCTGC GGGAAGCAGA TCTGCTCACG TACAGCTACA TCAAGAAGTT TGAGAGTTTG
    TGTGATGAAG CGGAGCTGGA AGCGCTGTGT GCTGAAGTGT CGTCTCAGAG TCCCCTGGGG
    GAGAGTCCAG ACTCCCCCAG CCGGCCCTGC TGCACCTTCA CATACATCAC TATGACTGGA
    GAGGAAGTGG AGCTGTGCCA GGGAGGACGT GACATCGCTG TCAGCTGGGA GAATAAGGAT
    GTGTATGTGC GAGCGGTGCG TGAGCTGCGT CTGCGGGAGC TGCAGAATGT GGAGTGTATG
    TCTGCGGTGC GCGCCGGTCT GGGCTCCATC ATCCCACTGC AGCTGCTGAC TTTACTGAGT
    CCCGCAGAGG TGGAGCTGCG CACCTGCGGT CTGCCGGACA TCAACCTGGA GTTCCTGAAG
    GCGCACACCA TGTATCAGGT GGGTCTGATG GAGACGGATC AGCATATCGA GTTCTTCTGG
    GCGGCGCTGG AGATGTTCAC GCAGGAGGAG CTCAGCAAGT TTATTAAGTT CGCCTGCAAC
    CAGGAGAGGA TTCCCTTCAC CTGCCCGTGC AAAGATGGGG GTCCGGACAC CGCACATGTG
    CCCCCCTATC CCATGAAGAT CGCCCCGCCG GACGGCACCG TGGGCTCTCC AGATCAGCGC
    TATATCCGCG TGGAGACGTG TATGTTCATG GTCAAGCTGC CGCAGTATTC CTCCCTGGAT
    GTGATGCTGG AGAAACTGCG ATACGCTATT CACTATCGAG AAGATCCTCT GAGCGGATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa51540
    Clone ID Related Accession (Same CDS sequence) XM_009303669.3 , XM_009303669.2
    Accession Version XM_009303669.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13254bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009303669.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    1021
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    1321
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    1501
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    1981
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    12181
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    12301
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    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGCTCAG GTGCTGTGGA TTCTTCCCAG TGGCTGTCAG TGAAGGAAGA GACTATTTTC 
    CTTCACGATG GACTAATTCG GGTCACGGAC CTGGCTGAGC TGCCCAGCGA AATCGGCGTG
    TCTGAACAAG CTGAATCCGA GCAGGAGATC TTGACGTTTG AGACCAAGAA TCCAGTGGAG
    CTAGCCGAGC GTCTCCGAGC CGTCTGTGGA AACCAGAGCA ACGCTTATGC ACGCCTTTTG
    GAGTATCGTC TGAATGCACT GCGTGGCCTG TGGGGGGCGC AGCGTCAGCT GGCCCTGGAG
    GAGCAGCAGG ATCATGACGG GGTTGTGTCA GCCAGCGGCG GAGGGTCCGA CGAGGAGACA
    CTGGCCCTAC TCAAACGACA GGGTTTACTG CAACCCCAAC ACCACCACCA TCCACACCAC
    CCTCCGATGG CCAGCGGCTC AGAGCAGGCA CCATTCACCT CCCGTGTGGG CCTGCTGTTG
    GTTTTCCCAC TGCTGCAGTC GCAAACTCGG CACGACCCTG CGCTGTGCGG CGTCACAGCG
    GAGGTGTTGC TCGCATGTCT GCGGGACTGC CAGCCACTCA GCCTGGGGAA GGAGCCCGCC
    GACTGTCTTA ATGGCCTGGA GAGGTTGCTG TGCTCCTGGT TGGAGGAGAC GGAGGGTTCG
    GCACCAATGA GGCCACTGCT CACCCAACAG CAGAGACAGA ACGCAGCTGC GGCGCTAGTG
    GCTCTGGCAT GTGCAAGGGG CTCCCTCAAA ACCTTCATCC ACACGGTCCA CCTGCTGCAG
    AAACAAACAG ACCTGGGTCA GCTTCCTGTG GCTGATGTTC TCTATCGACT GCTGCTTCTT
    GAAGGAGGTC CAGGTTCGCC CTCATGTCTG CTGGGGGGGA AACATATTGT GTCGTGGGGC
    TTTGAGGACA TGCTGCCCAC ACCAGAGAGC AGCGCCGCAG GAGCCGACAG TAAAGACACA
    GATCTGGGCC GCAGCCTGGC CACAGATGGA TTCTATCTCT ACACCACGAG CTCTTTTGGG
    AAGGGTTTGA GCAAGCTGGG CTCCGGGCAG CACGGGACGC TGAGGGGTTT TGTGTACTGT
    CGTAATGAGG AGATGGAGGC GGGCTGGGTG GCTCACAGCA GTGGATTTCT GCTCCACCGT
    CCTGCATCAT TTGACACTAA ACCTCATCAG CTGTGCCACG TCATCGACCC GTTCACACTG
    CAGGTTTCTC AGGTGTTAGC GATGCCCGTT CATCATTTCC CAGTGGGCAG CTGCCTGACC
    ACGCTGCGTC TGTGTTCTGA CGGCACATAC TTGTATTGGG TTTGGTCTCC GGTCAGCATC
    AACGAGAAAA CGCAGAAAGG CCACTCTGTC TTCATGGACG TCTTCCAGTT AACGAGTGAG
    TCCGGTGTGT GTGTGGCCAG TCCTGTACAA GACCGGGTGA TCCTGTTGCG AAAGGAAGGC
    GAGTCGTCCA AGTGCTTGAA TGAGTTGCTC CTCAGCCGAA TGTCGCGCTT CCGAGCCTCG
    CACTCGTCCA CACTGGCGGC TCTCACCGGC TCCGCCATCA CCAACCCTGT CAAAGAGGAG
    CAATCGGTGG TCAACACAAG CTGTGGTCTG TCCATCAAAA CACTGAGGAA GACACCCATG
    TATGTGTGCG GGACGTCCCT GGTGATGCTG GTGCCGACTC CAGGTGTTGC TGGGAGCTCC
    GCCACGCGCT CGCTGTTCGG GGGATCCAGT GGCCTTTCTT CTCTCAAGAT CTTGGCCTCT
    AGTTTGGTGT TTAACCTGGC GGACGGTCAG TTCAGCAATC GCGTGGATCT GGTCGACGCT
    CCGGGAAGTT CTCTGGGTCG TGGAGCTCAG GTTGCAGGAC TCGGCGCCTG TTATGATGCA
    CTGAACAACT TAATCTGGAC GTGTTCGAGC GACTACATGG ACCAGTGGTG CAACCCTGGA
    AATCAGGCCT TCCATCATGT GTGCCAGCGA CTCGGCGTCA GTCACGCCAT CAGCCAGACC
    AAAGAAGAGA TGATCGACAC ATCCAGCGTG ATCAGCCAGC TGCTCCATCA TGTGGGAGCC
    ATGTGTATCC ATCAGCTGAA CCTGCTGGCC GCCAGCAGTA GTTTACACTT GGGCACCTTC
    ATAGGGAAGC AGCCAATGGA GGCCTGTCAC TTCAGCTCCA TCTGTGACGT GATGGAGAAA
    GCCATGGTCA ACGGAGACAC CCGCATCATC CGCTGCATCC TGGTCGTTTT TCAGGTGGTT
    TTCCAGTTTT TTAATCCAAA CTCAGAGCAG AACCGGGAGT GTGTGAAGCG CTCAGGACTG
    CTGCTGTGGC AGTTACTCAT GGCTCCACCT GATCAGATCC AGCCGGAAAT ACAGAAAGAA
    GTTTGTCTCG CCATCAGCTC AGGTTTAAAC ACCCTGTATC AGAGCGAATC TGAGTTGAAC
    AACCTGCTGA AGTTTGTTTT CACAGAGGGG GAGAAGAACA GTGGTTTGTC TCAGTTACGA
    GACGTGATTT TAAACAATCT GGCTAACCAG TTACAGAAGA ACCGCTTTGG TAGTGAAGAT
    GATGATCACT ACAGGCTGAA GGATGAGCTG CTGCAGTGCA TCTTGAAGAC GGTGGTGCGC
    GAGTCGTGTC TCCTCATCAC CAAATGTCAA ACCGTTTCCA AAGAAGACCT TCAGAAGCTG
    CTCTCTACTG TACCTGTGGT GTCTCCCAGC TTGCGTTATT TAATGGCTGT GCAGAATCAT
    CTGCTCAGCA ACACCATCCT GCTGCACCCG GAGGACATGG AGGACAGCGA CAGCTCCCTG
    CAGGGGGAAA CAATGAAGGA GCTGCAGACG AGCATCTTGT CTCTTGCGTT GAAGATTCTG
    GTTGGATGTG ATGAAGTTCT GGAGACGCTT CAGCAGGTGA CCAGCTCTCT CATCAACAGC
    AGCATCAGCG ACCGAGAGAC CAGGCTCAGA GGTCTTGAAC AGGTGACCAA GGCCACCATG
    TTGGGTCATT TGCTGCCGGT TCTGCTGACG TCACTGATGC ACCCGAACCT CCAAACACTC
    ACGCTAGCAG ATGCGCTCAT GCCACAGCTA GTCCAGCTCG TCCTCTACAC TAGCCAGACC
    GCTTTGCTGC TAAAGACACA TTCTCCATTA TTTGTTGAGG ATTCCCCAAT AAATCAAGGC
    TCCAGCCAGC CCTCATTAAA TGACAGTTTT CAGATTCTGG AGGAGAGAGA GGAGCCTGGC
    TTTCTGACGG GGCTGAAGAT CCCCGCCCCG TGGGCTGCTG GGAAAACGGT GGAAACTGTT
    CACCCGGTGC GGGATAACTA TAAATTTAAA GACACAGTGC ACATTCCTGG AGCTCGCTGT
    CTGTACCTGC GCTTCGACCC GCGCTGCTCC TCACAGTACG ACTACGACAA GCTTGTGATT
    TACGCCGGGC CGAACACAAA CAGTCGTAAA GTAAGCGAGT ATGGAGGAAA CACCATGGGT
    TATGGCAGCC GCAGTGTATT GGGAACCGGA TGGCCAAAAG ATCTGGTCAA GGTGGAAGGA
    GATACGGTGA CGTTCTCTTT TGAGATGAGA AGTGGACGCG AGCACAACAC TCCAGATAAA
    GCCATGTGGG GTTTCTCGTG CACCGTCAGA GCGCAGGAGT CGTCTGAGGA TGTGTCTGGC
    GGTCTGCCGT TTCTGGCTGA TCTGGCTCTG GGTTTATCAG TGTTAGCCTG CGCTATGCTA
    CGAATCCTCT ATAATGGGCC TGAAATCACA CGAGAGGAAG AAGCTTGTCA CGACCTTCTC
    TGCTCCAAAT TACTGCAGAG GTGTCAGTGG CAGGTGGAGG CGAACGGAGC CATCTCTCCT
    GCACTCACGC CATCTCCGTC TCCTCTTCCT CTGACTATCG ATGAGGAGCG CGAGTTCACA
    TATCCATCAG ATATCCTCGC TCCGCCGCCG GGGCCCATCC CGAGCGCCTG CTGTGAGCTG
    CCCCGCATTC ACTTACCGCA GGGTATCATG GGCCGCCTGA GGGAGCTGTC TGGACGTGCT
    CGGCCACAGT TTCGCCCCAG CATCAAAGAG GTGATCCGTC CAGACGTGAT GGAGGAAGTG
    ATTGTGTCGT GTGTGATTAA ACACTTGGGT CTGGTGGACG CGCTGCAGTC GCTGGTCAAC
    TTCCAGTACA GAGAATCTCA CAGAGAAGAA CACAACCTGC TCTGCAAGAT CATGGCTGAG
    ACCTTCAAGA AGATCAACGC CATGGAGAGA CAGTTACAGA GCGTGGCTGA GCTGGAGCAG
    AAGTGGCAGA ATGAAGTAGA TGAAGCTCAG CAGGGAAAAC TGGAGAACAA CACTCCTTTC
    TTCCACGATT ATCACTTCTT TGAGAATAAA ATGAAGGAGC TGGAGCTTCT CTGTTCGCTA
    AAGGAGGTTC CTCTGGACTG CACTGATCCA GAAAACGCTG TGATGGCTTT GAGAGAGAAG
    TTTTTTCATG AGGTGAACTC CACGCTGCAG CAGCAGAGAA GTGTTGTTCC TCTGGCTAAA
    ACCAAAGCGC TTGTGAAGAG TTTGATCAAC CGCTCGGAGC TGCTGCTTCA TGTCACCATC
    GCTCCGCAGT GCAAGAGTCT GTCATCAACA CCCACAAGCA CACCGGCCTG TAAGTCGGTG
    TCAGACGCTA AATCGATGGC TCCCGTGGTG AAGCAGCCGG TGTTCCTGCG CAGCATGTCT
    GCTCCGTCAG ATCTGGAAAT GATCGCCAGC AGTGATGTGG AGTTCACACA CGGAGCGCAA
    AGGAGGCGGC AGCACCCCTC CAGCCACCGG AGCAGCTCAT TTACACTCCT GCAGTCATTG
    GTGATTGAAG ACAGTAAAGA TAAACCCACG TACAGTGTGC TGCTGGGACA ACTGTTCGCC
    TTTATAGGAA CCACTCCTGA CCAAACCGTG TCGAGCAGCA GTTTTCTGTC CGCAGCTCAG
    ACGCGCTGGA GAAGAGGACG AACACGCAAA CAGGCTCTGG TTCACATGCG GGAGCTGCTG
    ACCGCTGCCG TCCGCGTCGG TGGAGTCACT CATCTGGTCG GACCCGTCAC TATGGTCCTG
    CAGGGGGGAC CCAGGGTGGA GGAGCTCACC TGTGGTGGGA TGATGGAGCA GGTGCAGGAG
    GCGTTTGGAG AGACCATGAC GTCAGTGGTG TCTCTGTGTG CCCGATACCC CATCGCCTGT
    GCCAACAGCA TCGGCCTGCT CTGCACCATC CCCTACACCA GGAGTGAGGA GCAGTGTCTG
    GTGCGCAGCG GTCTGGTTCA GCTCATGGAT CGCCTCTGCA GTCTGAGCAG TCAGAGAGAC
    TGCAGCTCCA ACGAGAAGCA AACCAAAAAG CAGAAAGTGG CCACCATGGC CTGGGCTGCG
    TTTCAGGTGC TGGCCAACCG CTGCATTGAG TGGGAGAAAG TCGAAGGAGG CTCCACAGAC
    GCAGTGCATT CAGGGTTGGC TCGACAGGTG TCCAGCCTTC TGACCAATCA TCTGGCTCGA
    GCGACAGACT GCTGTGGAAA CCAGGCGGCA GGAAATGATG CGCTGCAGGA TGTGCTCAGT
    TTACTCAACG ATCTGTCCAG GAGTCACATA GGCAAGACCA TCCTGAGCCA GCCTGCCTGT
    GTGTCCAAGC TGCTGTCTCT GCTTCTGGAC CAGCGACCGT CGCCCAAACT GGTGCTGATC
    ATCCTGCAGC TGTGCCGCGC CGCGCTGCCC CTCATGAGTG TGGAGGATTG TGGAAATGTA
    GCGCTGCCGT CCTGGAGCTA CTCCACAAAC GCACTGGACG CCGAGCAGCA GGACGCCAGC
    GACCCTGCCT TCCGCATCGC ATCTCTGCTG CTCGCCAAAC TGGCCGACTA TGTGGTGCCA
    GGCTGTCAGA CGTTGCTCTC TCCCAGCGCC TCTGAGCCGG ACACCAGTCT GAGCCGAGCC
    TGTCCCAAGA GCAGCTCTAA GACCGACAGA GATGCCAGCG AGGAGGGCGA AGCGGTGGAC
    GGAAAACTCT CCATATTCAT CCATAAGAGA GAAGACCAGT CGTCGCACGA GGTGCTGCAG
    CCTCTGCTCA GCAGCTCAGA GGGTCGACCG TTTCGTCTCG GCACTGGAGC CAACATGGAG
    AAGGTGGTGA AGATGGATCG AGACATGACC AAGAGCGGCT GCTGTGAGGT CATCACTGAA
    GAGGCGTCCG CTGCTCTCAG GAAAGCCAAT AAATGGGCTC AGTCGGGGCT CATTGTGAGT
    GTGGGTCCGC CCATCGAAAG CAGCGCTCAG GAGAACTCAG GAGCGACCTC TACAGGAGAC
    AAGAAGAAAA GCGCTCAGTC CACGGTGTGT CGAGAGAGGA ACGCTGAGCT CGCCCGGTCT
    GACCCGGTGC GTCCGTTCAT CAGTGGTCAC GTAGCCAACA GTATGGCAGC AGAGGTCATC
    GCTCTGCTGC ACAGCCTCCT GACGGCCCCC GAGTCCAACA CTGCTCAGAT CTGGACAAGC
    ACTGCAGAAA AGGTGTTGTC CAGAGCTCTG ATGTTCATCC CTCAGCTGGG GAAGTTTGCT
    GAGAGTATTC TGGAGAGCGG AAACAGCAGC GGCAGGAAGT TGTCCAAGCT GCAGGCCATT
    GGTCGACAGG CGGTGGCGGC TCTCTGTGCG CTCGGCGGAT TCAAGGAGAC CATCAAGATT
    GGATCTGAAG TGCAGGTGGT TGGTAAAGGT GTGTTGGACA GTGTCGGTGT GGTTATATCC
    ATTAATGAGC AGGAGGGCAT CGCCACCGTC AAGTTTCCAT CCTGCGAGTA TCGGCGCTCC
    AGCAAAGCAT CTGATATCCT GACGGTGCCC ATATCACGCC TGTGCACGCC GCGCTCAGAG
    GCTCTGCCGC TGCACAAGCT TTCCATCACA GAGAAGGTTG TTCAGGCCGT TCAGTCCATG
    TTGCTGCCGC AGGAGGGCAG TCTGTCCATA CACACGTCTC TGCCGGCGTC TGGAGACGGC
    TCCAGTCCTG TGATGGCAGT GGTGCGTCTG CTGGCCGAGA TCAGAACCAG AGCGTGTCTG
    GTGATGGCGC AGCTGCTGGA GGACAGCTCT TTCTGTGAGG AGTTTATCCA GCAGTGTCCT
    GCAGCCGTGG AGGTGCTCAA CCTCATCGCT CAAGAGTGCA GTCCTGGAGA GCGTTTGGTG
    ATGGTGGAGG CTCAGTGTGA GCGTCTGCGC ATGCTTTATC GAGACTGTGC TCGTCCTCCA
    CCACCGCCTG TGCAAACTGA CCAACGGCAG CCGAAGGAGA TCACGTGGTG CCCGTCTCGA
    GTCTTCCCTC CGGTTCGGGC CTGTATGTTT TCCTCGCACT TGACGTCGGT GACGTTTCTG
    GCTGATCCGA GTGCAGGCGG AGGCCTTCCC AGAGGAACCT TCATCTACGC CACTTCACCC
    GTGCCCATTC AGGCTCCGTC TTTTTACTGG GAGATCGAGA TCGTTTCGTT CGGAGACTCG
    GAGGACGACA GCGGTCCAAT AGTGTCCTTT GGATTTTCCC CGGAGGCGGA GAAGAGAGAC
    GGAGCCTGGA CCAATCCCGT CGGGACCTGC CTGTTTCACA ATAATGGCCG GGCGGTTCAC
    TACAACGGCT CCAGTTTACT GCAGTGGAAA AGTGTGAGGC TCGACGTGGC TCTGCTTCCT
    GGTGATGTAG CCGGCATCGG CTGGGAGCGT CTGGAAGGCA CTCCTCCTCC ACCCGGTCAG
    CCTCCCAAAG GAAGAGTGTA CTTCACCTAC TGCGGTCAGC GACTCGCTCC CTACCTAGAG
    GATGTGGCTG GAGGCATGTG GCCCGTCGTT CACATCCAGA AGAAGAATAC GAAGATTCAC
    GCCAACTTCG GCTGCCGGTT GTTTGCGTAC GCTGAAGGCC AGGCTCACAG AAACGCTGCT
    GATCTCTGCA TGGATCTGTC AGAGGAGATC AGTGCTAACT TCGAGGCTCT GCCCTTCGCC
    ATGGCCTCCG ACAGCGATAA TGATGCGGGC ACCAGTGTGG CGTCTGACTC CGGCTCCCAC
    GGGCCTCCTT GTCGCATCGC TGCCGTCGTC ACAGCACAAC AGCAATACAA CAGCGATGCT
    TCTTGCCACT ACAAGGTAGA GCTGAGCTAT GAGAATCTGG TGGTGTCGGG ACCGAACGCT
    CATCCGCCAC CCATCGCTGA TGACGAGAGC GATGATGAGG ATGATGAAGA CATTCCCCGG
    GAGGATCATT ACGCCCTGCT AGTGAAAGCG TGGGAAACTA AAGTGTTTCC CACCATTCGC
    CGGCGGTTCA GGAACGAGGC GGAGAGGAAA TCTGGCCTGG ATCAGATCAA GGGAGCGCTG
    CAGCTCGGTA TGGTGGACAT CGCTCGTCAG ACGGTTGAGT TTCTGTATGA AGAGAATGGC
    GGGATCCCTC GAGACCTCTA CCTTCCAACC ATCGAAGACA TTAAGGATGA AGCAAACAAG
    TTCACCATCG ACAAAGTCCG CAAAGGCTTG ACGGTGGGCA TCCGCTGTCC AGAGGGAAAC
    AACACCTGCA GCAGCACAGG AGGAACCGCT CTACCAAAGT TTGCCATCCG CGGCATGTTG
    AAGACCTTCA GCTTACATGG AGTTGTGCTC GATGTGGATT CGGGTAATGA GCTGGTGCAG
    GTGGAGACGT ATCTGCGCAG TGAGGGTGTG TTGGTGCGCT ACTGGTACCC CATAGAGATG
    CTGGAGAGAC CGCCGGCAGG ATCCCGCAGG GCTGCAGCCA ATGGCCTGGT GTCTTTGGAC
    AGCAGCAACA TCCAGATTCA TCGGGAGCTG CTGCGCTGTG AAAGTGCTCT GGCGCGTCTG
    TACTGCCGCA TGGCGCTGCT CAACATCTTT GCGCCCAAGA GTCCTCACAC ATTCACGCGC
    CTCTTCCACA TCCCTGCAGT GCGTGACATC ACACTCGAAC ACCTGCAGCT CCTGTCCAAT
    CAGCTGCTGG CTCCGCCTCT GCCAGACGGC CGGATCAGCT GTAACTCTGT GCTGCTGGCT
    CAGTCGGTCC TACATGAACT GCAGGGTCAG AGCTGCTCTC CCACTGAGCT CTTCTATCAG
    GGAAACGCTC AGAGTGTCCG CGAGTGGCTG AGCGTGGCCA TCAGCAGAGC TCTGCATCAG
    GGGGAGGACA GTCTGCTGGA GCTCACCAAA CAGATCTGCT GCTTCCTGCA GAGTGCGCCT
    GAGCAGTTCA CGCTGGAGGA GATTCCCATC ACCGAGTCAA AGGTCAGCAT GGACGTCAAC
    TTCCCCGGCG CTGCGTTTGT GCTCGTCTCC TGCAAAGAGA GTCAACTCAG CTGCCGCAAA
    GATTCCTCTC TGTACAAAGC TCCATGGGCT CGAGTGATGG TGTATGGGCT CGGTCATAAA
    GTGCGGCGTA ACGGCCAGCT GAACCTGATG GAGGCCGTGT GTTTCCCGCT GGACGCCTCT
    CCGTCCAACA CGGGCCTCAC TCCTCCACCC ACAGCCAACC AGTATCCCAG CATCATCATC
    CCCACCGACA GAGTGCACGT CAAACTGGGT GTGTCTCCCC CTCCTGGAGC AGTGCTGGTG
    TTGCACTCGT TGCCTCTTGA GTTTCCATTG GCGATGGCCT TCGCTGAACA GCTGCTAACC
    TACAGTGTAG GAGAGACCGT TGAGCGCTCT GAGGATGAGC TGGACACGGT GCCCACCTCT
    GTGCTCATGC AGGTCGTGGA GCTGCTAGGT GGCCTTTTGT GGACCACAGA TCTTGCTCCG
    AGCATCAAGG AGCTGACCTT CCACCTGCTT GCAGAGCTCC TCCGCAAGAT CCACGCTTTT
    GAGCGCCGCA AATCCCCTTC GGGTCTCTCT AGCCACATCA CGCTGCTTCT CAACCCCTGC
    CTAACAGTCC TGATGGTTCT TCAAACCGAA CTACGAAAGC TTTACGACCA CGAGACCCAA
    AGCTGGGGGT TGGTTAGCGG AGGATCCTGC ACTTCCGCAA CAGGCGGCAG TGGAGCGCTT
    GCACTTTCTG CAGAGCGGAG CCGCTTCTCT ACATACTTCC ACGCACTGAT GGAGGTTTGC
    CTGGCAGTGG CGGAAGTCAC TTTACCGCTG AGTTCTGGTT CTGGGACGTC AGTAATCGGC
    GTCCCTTCAT CAACAAGCAC AGCCAACTGC AGTGATTCCT CATCCTCTTC TTCGTCCTCT
    CCAGGCCAGA CGCCGCAAAG TCCGAGTTTG CTTTCAAAGC GCAAAAAGGT GAAGCTAAAA
    CGTGAGCGCG CTGCAGTCGG CTCAGCTTCC GCCGTCTCAG GGAAAAGTGG CTCGAGATCA
    TCAGAATGCG ACAATGCTCT ATTAAACATG AAGCCTGAAG ACATGTTGTG GTTTCACCGG
    GGGTTGACCT TGCTTTTGAT TTTACGGCAT CTTACTAATA AGGACCCGCA AGGTCTTGGC
    GTCACCGGCG ACGCTGTGGC AGACGCTTGC CAAGCTTTGG TTGGCTCGAC AGCTCACAGT
    CGCTTGCTGG TGCTCTCAGG AATCCCGACT CACCTGGAGG AGTCTGTGGT GCGCAATGCA
    ATACGAAGAG CATGCAATGC TCATGGCGGG CTCTTCAGGG ATGAGATCTT CATTCCAGTT
    CAGGAAGAAG ACCCTAAAAA AGCCAAAGCG GAAACGACAT GTGTTCCTCA AGATGTAAAG
    AGAGAATGCA CTGCAGGAAG CCCCAACAGC AACAGTGCGG CAATGAGTGT CTCCAGCGCT
    TTACAGGCAG CCTTCAGAGC CTCCGGAGAG CCCTTGTCCA ATCAGGAGAT GGTAAACAGT
    CCTTCCCAGC AACAAGCACC TCTGGATCCA CTCACAGTGT CCAGTCAGGA GAGTCTGGAC
    GTGTCTCTGT GTAGCACAGA AAGTCAAGGG AGTCTCGGCA GCCTCGGGGA GCAGGATAGT
    GCTGCCACGT CAGTCTCAGA TGGGAGCTCC ATGTACACGG TGGCCTCACC TGATGTTCAT
    GCCAGTGTGA CGCGTCCCAT AAAAGGCTAT GCTGTCATTG AGGTAAGGGC ACGAGCTCAG
    GTGGAGAAGA TTCGGGCCAG TCTTTTCAAC AGCAGCGATC TGATTGGCCT CTCCAATCTG
    GAAAGGGAGG AGGAGCTAAT GGAGATGACC AATGAGGAGA TCCTCACCGC CTCCAGTGTC
    AATCAGAGTT TATTTGACAC ACAGGGCAGC TCTGCTCTGG AGGAGTACTT CCTGGATAAA
    TCCATCAGAG GAGACAAGCT GGTTCCTGGA GCTCGTGACG TGCTGACAGA CATCTTTAAG
    AGCTGCATAT ACGCAGAGCA GGCGCTGAGC CCCGTTCCCG TGAAACCTGT CAAAGTCTCA
    GACATTTACC TCAGCAAAGA GCAGATCAAC TCTCAGTCTC CCGGAAACCT GCTACACGTG
    TTTTTCACAC ACGTTCGGCC TCCCAAAAAA GTGCTCGATG ACCAGCTCAC ACAGATTCTG
    CGGAAATACG GCACAGTAAA GCCCAATAAA AACAAGCACA GCAAAGCGGG CAAAGAGCAG
    CACCAAGGCA AAGTGGTGAG CACAAAAAGA CCCATCACCA AACCTCCGGC CAAAGAGAAG
    TCCATGCTGA ACAGCGTGCG AACTGTACTG AGTGAGAAAA AAACCGTCCT CAAGCCAAAA
    TCTCCAGAGA AGACCAAACC CGATGAAAAA GACCCCGAAA AATCTCCTGC CAAAAAACCC
    GAAGTGCCAG AGGAGAAGTT TTTGACGCTG GAGGGTTTCC ACAAGTTTAC TCTGGACAGA
    GCCAAGCAGG ATATCCGCAG TGTTTGGCGG GCGATCCTGG CCTGTGGATA TGACCTGCAC
    TTTGACAGAT GCACATGCAT TGATTCCCGA CATGCTCAGA AAGCCAGCAG AAAATGGAGC
    TTCGAGATGG ACTTTGCGTT GGTGCAGTTT GTGAACCGTC TCTGTCGTCA CCTGGCCATC
    ACTCCTGCCC GCCTGCATCC ACATGAGGTT TACCTGGACG CCAGCAAAGA TGGCGCGGAT
    CCTGAGGTCG CGTGTCTGTT AGGTGTCCCA GTGGAGAGTT TGCGTCTGCG CTTTGCTCTG
    CTGCAGTCCC TCAACAGCAC GCTGGAGAGC TTCTTCCTGC CTTTAGTGGA GCTGAGACTC
    ACACAGACCT ACCAGAACAG CATCGCCACA CTTCTGTGCG AGGCCAAAGG CCTGATATTT
    TACGACACTA AGGTGACGGT GATGAACAGG GTGTTGAACG CTACCGTGCA GCGGACGGCA
    GATCACGCAG CGCCTGAAAT CACTCTCGAC CCGCTGGAGA TTGTTGGAGG AGAGATGCGC
    GCTGCGGAGA ACACTTATTT CTGCCAGGCA GCGCGGCAGC TGTCGTGTGT GCCATCGTCT
    CAGCTGTGTG TGCGCATCGC CAGCGGAGGA GACCCCACAT ACGCCTTCAA CATCCGCTTC
    ACCGGAGAGG AGGTCCACGG CACCAGTGGC TCTTTCAGGC ATTTCCTATG GCAGGTGTGT
    AAGGAGCTCC AGAGTTCAGC TCTCTCTCTT CTTCTGCCCT GCCCGAGCGC CGCCGCTAAC
    CGCAATAAGG GGAAGTACAT CCTGACTCCG TGTCCCATCA CATACGCTGA GGAGCAGCTG
    CTGAACTTCT TCGGGCAGCT TCTGGGCATC GCCATCCGGG CCGATGTGCC GCTGCCTCTG
    GACCTGCTGG GATCCTTCTG GAAGGGTCTG GTCGGAGAGG CTCTCGATCC CGAAGCTGAC
    CTGCGGGAAG CAGATCTGCT CACGTACAGC TACATCAAGA AGTTTGAGAG TTTGTGTGAT
    GAAGCGGAGC TGGAAGCGCT GTGTGCTGAA GTGTCGTCTC AGAGTCCCCT GGGGGAGAGT
    CCAGACTCCC CCAGCCGGCC CTGCTGCACC TTCACATACA TCACTATGAC TGGAGAGGAA
    GTGGAGCTGT GCCAGGGAGG ACGTGACATC GCTGTCAGCT GGGAGAATAA GGATGTGTAT
    GTGCGAGCGG TGCGTGAGCT GCGTCTGCGG GAGCTGCAGA ATGTGGAGTG TATGTCTGCG
    GTGCGCGCCG GTCTGGGCTC CATCATCCCA CTGCAGCTGC TGACTTTACT GAGTCCCGCA
    GAGGTGGAGC TGCGCACCTG CGGTCTGCCG GACATCAACC TGGAGTTCCT GAAGGCGCAC
    ACCATGTATC AGGTGGGTCT GATGGAGACG GATCAGCATA TCGAGTTCTT CTGGGCGGCG
    CTGGAGATGT TCACGCAGGA GGAGCTCAGC AAGTTTATTA AGTTCGCCTG CAACCAGGAG
    AGGATTCCCT TCACCTGCCC GTGCAAAGAT GGGGGTCCGG ACACCGCACA TGTGCCCCCC
    TATCCCATGA AGATCGCCCC GCCGGACGGC ACCGTGGGCT CTCCAGATCA GCGCTATATC
    CGCGTGGAGA CGTGTATGTT CATGGTCAAG CTGCCGCAGT ATTCCTCCCT GGATGTGATG
    CTGGAGAAAC TGCGATACGC TATTCACTAT CGAGAAGATC CTCTGAGCGG ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301944.1
    CDS431..13684
    Translation

    Target ORF information:

    RefSeq Version XM_009303669.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio HECT domain E3 ubiquitin protein ligase 4 (hectd4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303669.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGCTCAG GTGCTGTGGA TTCTTCCCAG TGGCTGTCAG TGAAGGAAGA GACTATTTTC 
    CTTCACGATG GACTAATTCG GGTCACGGAC CTGGCTGAGC TGCCCAGCGA AATCGGCGTG
    TCTGAACAAG CTGAATCCGA GCAGGAGATC TTGACGTTTG AGACCAAGAA TCCAGTGGAG
    CTAGCCGAGC GTCTCCGAGC CGTCTGTGGA AACCAGAGCA ACGCTTATGC ACGCCTTTTG
    GAGTATCGTC TGAATGCACT GCGTGGCCTG TGGGGGGCGC AGCGTCAGCT GGCCCTGGAG
    GAGCAGCAGG ATCATGACGG GGTTGTGTCA GCCAGCGGCG GAGGGTCCGA CGAGGAGACA
    CTGGCCCTAC TCAAACGACA GGGTTTACTG CAACCCCAAC ACCACCACCA TCCACACCAC
    CCTCCGATGG CCAGCGGCTC AGAGCAGGCA CCATTCACCT CCCGTGTGGG CCTGCTGTTG
    GTTTTCCCAC TGCTGCAGTC GCAAACTCGG CACGACCCTG CGCTGTGCGG CGTCACAGCG
    GAGGTGTTGC TCGCATGTCT GCGGGACTGC CAGCCACTCA GCCTGGGGAA GGAGCCCGCC
    GACTGTCTTA ATGGCCTGGA GAGGTTGCTG TGCTCCTGGT TGGAGGAGAC GGAGGGTTCG
    GCACCAATGA GGCCACTGCT CACCCAACAG CAGAGACAGA ACGCAGCTGC GGCGCTAGTG
    GCTCTGGCAT GTGCAAGGGG CTCCCTCAAA ACCTTCATCC ACACGGTCCA CCTGCTGCAG
    AAACAAACAG ACCTGGGTCA GCTTCCTGTG GCTGATGTTC TCTATCGACT GCTGCTTCTT
    GAAGGAGGTC CAGGTTCGCC CTCATGTCTG CTGGGGGGGA AACATATTGT GTCGTGGGGC
    TTTGAGGACA TGCTGCCCAC ACCAGAGAGC AGCGCCGCAG GAGCCGACAG TAAAGACACA
    GATCTGGGCC GCAGCCTGGC CACAGATGGA TTCTATCTCT ACACCACGAG CTCTTTTGGG
    AAGGGTTTGA GCAAGCTGGG CTCCGGGCAG CACGGGACGC TGAGGGGTTT TGTGTACTGT
    CGTAATGAGG AGATGGAGGC GGGCTGGGTG GCTCACAGCA GTGGATTTCT GCTCCACCGT
    CCTGCATCAT TTGACACTAA ACCTCATCAG CTGTGCCACG TCATCGACCC GTTCACACTG
    CAGGTTTCTC AGGTGTTAGC GATGCCCGTT CATCATTTCC CAGTGGGCAG CTGCCTGACC
    ACGCTGCGTC TGTGTTCTGA CGGCACATAC TTGTATTGGG TTTGGTCTCC GGTCAGCATC
    AACGAGAAAA CGCAGAAAGG CCACTCTGTC TTCATGGACG TCTTCCAGTT AACGAGTGAG
    TCCGGTGTGT GTGTGGCCAG TCCTGTACAA GACCGGGTGA TCCTGTTGCG AAAGGAAGGC
    GAGTCGTCCA AGTGCTTGAA TGAGTTGCTC CTCAGCCGAA TGTCGCGCTT CCGAGCCTCG
    CACTCGTCCA CACTGGCGGC TCTCACCGGC TCCGCCATCA CCAACCCTGT CAAAGAGGAG
    CAATCGGTGG TCAACACAAG CTGTGGTCTG TCCATCAAAA CACTGAGGAA GACACCCATG
    TATGTGTGCG GGACGTCCCT GGTGATGCTG GTGCCGACTC CAGGTGTTGC TGGGAGCTCC
    GCCACGCGCT CGCTGTTCGG GGGATCCAGT GGCCTTTCTT CTCTCAAGAT CTTGGCCTCT
    AGTTTGGTGT TTAACCTGGC GGACGGTCAG TTCAGCAATC GCGTGGATCT GGTCGACGCT
    CCGGGAAGTT CTCTGGGTCG TGGAGCTCAG GTTGCAGGAC TCGGCGCCTG TTATGATGCA
    CTGAACAACT TAATCTGGAC GTGTTCGAGC GACTACATGG ACCAGTGGTG CAACCCTGGA
    AATCAGGCCT TCCATCATGT GTGCCAGCGA CTCGGCGTCA GTCACGCCAT CAGCCAGACC
    AAAGAAGAGA TGATCGACAC ATCCAGCGTG ATCAGCCAGC TGCTCCATCA TGTGGGAGCC
    ATGTGTATCC ATCAGCTGAA CCTGCTGGCC GCCAGCAGTA GTTTACACTT GGGCACCTTC
    ATAGGGAAGC AGCCAATGGA GGCCTGTCAC TTCAGCTCCA TCTGTGACGT GATGGAGAAA
    GCCATGGTCA ACGGAGACAC CCGCATCATC CGCTGCATCC TGGTCGTTTT TCAGGTGGTT
    TTCCAGTTTT TTAATCCAAA CTCAGAGCAG AACCGGGAGT GTGTGAAGCG CTCAGGACTG
    CTGCTGTGGC AGTTACTCAT GGCTCCACCT GATCAGATCC AGCCGGAAAT ACAGAAAGAA
    GTTTGTCTCG CCATCAGCTC AGGTTTAAAC ACCCTGTATC AGAGCGAATC TGAGTTGAAC
    AACCTGCTGA AGTTTGTTTT CACAGAGGGG GAGAAGAACA GTGGTTTGTC TCAGTTACGA
    GACGTGATTT TAAACAATCT GGCTAACCAG TTACAGAAGA ACCGCTTTGG TAGTGAAGAT
    GATGATCACT ACAGGCTGAA GGATGAGCTG CTGCAGTGCA TCTTGAAGAC GGTGGTGCGC
    GAGTCGTGTC TCCTCATCAC CAAATGTCAA ACCGTTTCCA AAGAAGACCT TCAGAAGCTG
    CTCTCTACTG TACCTGTGGT GTCTCCCAGC TTGCGTTATT TAATGGCTGT GCAGAATCAT
    CTGCTCAGCA ACACCATCCT GCTGCACCCG GAGGACATGG AGGACAGCGA CAGCTCCCTG
    CAGGGGGAAA CAATGAAGGA GCTGCAGACG AGCATCTTGT CTCTTGCGTT GAAGATTCTG
    GTTGGATGTG ATGAAGTTCT GGAGACGCTT CAGCAGGTGA CCAGCTCTCT CATCAACAGC
    AGCATCAGCG ACCGAGAGAC CAGGCTCAGA GGTCTTGAAC AGGTGACCAA GGCCACCATG
    TTGGGTCATT TGCTGCCGGT TCTGCTGACG TCACTGATGC ACCCGAACCT CCAAACACTC
    ACGCTAGCAG ATGCGCTCAT GCCACAGCTA GTCCAGCTCG TCCTCTACAC TAGCCAGACC
    GCTTTGCTGC TAAAGACACA TTCTCCATTA TTTGTTGAGG ATTCCCCAAT AAATCAAGGC
    TCCAGCCAGC CCTCATTAAA TGACAGTTTT CAGATTCTGG AGGAGAGAGA GGAGCCTGGC
    TTTCTGACGG GGCTGAAGAT CCCCGCCCCG TGGGCTGCTG GGAAAACGGT GGAAACTGTT
    CACCCGGTGC GGGATAACTA TAAATTTAAA GACACAGTGC ACATTCCTGG AGCTCGCTGT
    CTGTACCTGC GCTTCGACCC GCGCTGCTCC TCACAGTACG ACTACGACAA GCTTGTGATT
    TACGCCGGGC CGAACACAAA CAGTCGTAAA GTAAGCGAGT ATGGAGGAAA CACCATGGGT
    TATGGCAGCC GCAGTGTATT GGGAACCGGA TGGCCAAAAG ATCTGGTCAA GGTGGAAGGA
    GATACGGTGA CGTTCTCTTT TGAGATGAGA AGTGGACGCG AGCACAACAC TCCAGATAAA
    GCCATGTGGG GTTTCTCGTG CACCGTCAGA GCGCAGGAGT CGTCTGAGGA TGTGTCTGGC
    GGTCTGCCGT TTCTGGCTGA TCTGGCTCTG GGTTTATCAG TGTTAGCCTG CGCTATGCTA
    CGAATCCTCT ATAATGGGCC TGAAATCACA CGAGAGGAAG AAGCTTGTCA CGACCTTCTC
    TGCTCCAAAT TACTGCAGAG GTGTCAGTGG CAGGTGGAGG CGAACGGAGC CATCTCTCCT
    GCACTCACGC CATCTCCGTC TCCTCTTCCT CTGACTATCG ATGAGGAGCG CGAGTTCACA
    TATCCATCAG ATATCCTCGC TCCGCCGCCG GGGCCCATCC CGAGCGCCTG CTGTGAGCTG
    CCCCGCATTC ACTTACCGCA GGGTATCATG GGCCGCCTGA GGGAGCTGTC TGGACGTGCT
    CGGCCACAGT TTCGCCCCAG CATCAAAGAG GTGATCCGTC CAGACGTGAT GGAGGAAGTG
    ATTGTGTCGT GTGTGATTAA ACACTTGGGT CTGGTGGACG CGCTGCAGTC GCTGGTCAAC
    TTCCAGTACA GAGAATCTCA CAGAGAAGAA CACAACCTGC TCTGCAAGAT CATGGCTGAG
    ACCTTCAAGA AGATCAACGC CATGGAGAGA CAGTTACAGA GCGTGGCTGA GCTGGAGCAG
    AAGTGGCAGA ATGAAGTAGA TGAAGCTCAG CAGGGAAAAC TGGAGAACAA CACTCCTTTC
    TTCCACGATT ATCACTTCTT TGAGAATAAA ATGAAGGAGC TGGAGCTTCT CTGTTCGCTA
    AAGGAGGTTC CTCTGGACTG CACTGATCCA GAAAACGCTG TGATGGCTTT GAGAGAGAAG
    TTTTTTCATG AGGTGAACTC CACGCTGCAG CAGCAGAGAA GTGTTGTTCC TCTGGCTAAA
    ACCAAAGCGC TTGTGAAGAG TTTGATCAAC CGCTCGGAGC TGCTGCTTCA TGTCACCATC
    GCTCCGCAGT GCAAGAGTCT GTCATCAACA CCCACAAGCA CACCGGCCTG TAAGTCGGTG
    TCAGACGCTA AATCGATGGC TCCCGTGGTG AAGCAGCCGG TGTTCCTGCG CAGCATGTCT
    GCTCCGTCAG ATCTGGAAAT GATCGCCAGC AGTGATGTGG AGTTCACACA CGGAGCGCAA
    AGGAGGCGGC AGCACCCCTC CAGCCACCGG AGCAGCTCAT TTACACTCCT GCAGTCATTG
    GTGATTGAAG ACAGTAAAGA TAAACCCACG TACAGTGTGC TGCTGGGACA ACTGTTCGCC
    TTTATAGGAA CCACTCCTGA CCAAACCGTG TCGAGCAGCA GTTTTCTGTC CGCAGCTCAG
    ACGCGCTGGA GAAGAGGACG AACACGCAAA CAGGCTCTGG TTCACATGCG GGAGCTGCTG
    ACCGCTGCCG TCCGCGTCGG TGGAGTCACT CATCTGGTCG GACCCGTCAC TATGGTCCTG
    CAGGGGGGAC CCAGGGTGGA GGAGCTCACC TGTGGTGGGA TGATGGAGCA GGTGCAGGAG
    GCGTTTGGAG AGACCATGAC GTCAGTGGTG TCTCTGTGTG CCCGATACCC CATCGCCTGT
    GCCAACAGCA TCGGCCTGCT CTGCACCATC CCCTACACCA GGAGTGAGGA GCAGTGTCTG
    GTGCGCAGCG GTCTGGTTCA GCTCATGGAT CGCCTCTGCA GTCTGAGCAG TCAGAGAGAC
    TGCAGCTCCA ACGAGAAGCA AACCAAAAAG CAGAAAGTGG CCACCATGGC CTGGGCTGCG
    TTTCAGGTGC TGGCCAACCG CTGCATTGAG TGGGAGAAAG TCGAAGGAGG CTCCACAGAC
    GCAGTGCATT CAGGGTTGGC TCGACAGGTG TCCAGCCTTC TGACCAATCA TCTGGCTCGA
    GCGACAGACT GCTGTGGAAA CCAGGCGGCA GGAAATGATG CGCTGCAGGA TGTGCTCAGT
    TTACTCAACG ATCTGTCCAG GAGTCACATA GGCAAGACCA TCCTGAGCCA GCCTGCCTGT
    GTGTCCAAGC TGCTGTCTCT GCTTCTGGAC CAGCGACCGT CGCCCAAACT GGTGCTGATC
    ATCCTGCAGC TGTGCCGCGC CGCGCTGCCC CTCATGAGTG TGGAGGATTG TGGAAATGTA
    GCGCTGCCGT CCTGGAGCTA CTCCACAAAC GCACTGGACG CCGAGCAGCA GGACGCCAGC
    GACCCTGCCT TCCGCATCGC ATCTCTGCTG CTCGCCAAAC TGGCCGACTA TGTGGTGCCA
    GGCTGTCAGA CGTTGCTCTC TCCCAGCGCC TCTGAGCCGG ACACCAGTCT GAGCCGAGCC
    TGTCCCAAGA GCAGCTCTAA GACCGACAGA GATGCCAGCG AGGAGGGCGA AGCGGTGGAC
    GGAAAACTCT CCATATTCAT CCATAAGAGA GAAGACCAGT CGTCGCACGA GGTGCTGCAG
    CCTCTGCTCA GCAGCTCAGA GGGTCGACCG TTTCGTCTCG GCACTGGAGC CAACATGGAG
    AAGGTGGTGA AGATGGATCG AGACATGACC AAGAGCGGCT GCTGTGAGGT CATCACTGAA
    GAGGCGTCCG CTGCTCTCAG GAAAGCCAAT AAATGGGCTC AGTCGGGGCT CATTGTGAGT
    GTGGGTCCGC CCATCGAAAG CAGCGCTCAG GAGAACTCAG GAGCGACCTC TACAGGAGAC
    AAGAAGAAAA GCGCTCAGTC CACGGTGTGT CGAGAGAGGA ACGCTGAGCT CGCCCGGTCT
    GACCCGGTGC GTCCGTTCAT CAGTGGTCAC GTAGCCAACA GTATGGCAGC AGAGGTCATC
    GCTCTGCTGC ACAGCCTCCT GACGGCCCCC GAGTCCAACA CTGCTCAGAT CTGGACAAGC
    ACTGCAGAAA AGGTGTTGTC CAGAGCTCTG ATGTTCATCC CTCAGCTGGG GAAGTTTGCT
    GAGAGTATTC TGGAGAGCGG AAACAGCAGC GGCAGGAAGT TGTCCAAGCT GCAGGCCATT
    GGTCGACAGG CGGTGGCGGC TCTCTGTGCG CTCGGCGGAT TCAAGGAGAC CATCAAGATT
    GGATCTGAAG TGCAGGTGGT TGGTAAAGGT GTGTTGGACA GTGTCGGTGT GGTTATATCC
    ATTAATGAGC AGGAGGGCAT CGCCACCGTC AAGTTTCCAT CCTGCGAGTA TCGGCGCTCC
    AGCAAAGCAT CTGATATCCT GACGGTGCCC ATATCACGCC TGTGCACGCC GCGCTCAGAG
    GCTCTGCCGC TGCACAAGCT TTCCATCACA GAGAAGGTTG TTCAGGCCGT TCAGTCCATG
    TTGCTGCCGC AGGAGGGCAG TCTGTCCATA CACACGTCTC TGCCGGCGTC TGGAGACGGC
    TCCAGTCCTG TGATGGCAGT GGTGCGTCTG CTGGCCGAGA TCAGAACCAG AGCGTGTCTG
    GTGATGGCGC AGCTGCTGGA GGACAGCTCT TTCTGTGAGG AGTTTATCCA GCAGTGTCCT
    GCAGCCGTGG AGGTGCTCAA CCTCATCGCT CAAGAGTGCA GTCCTGGAGA GCGTTTGGTG
    ATGGTGGAGG CTCAGTGTGA GCGTCTGCGC ATGCTTTATC GAGACTGTGC TCGTCCTCCA
    CCACCGCCTG TGCAAACTGA CCAACGGCAG CCGAAGGAGA TCACGTGGTG CCCGTCTCGA
    GTCTTCCCTC CGGTTCGGGC CTGTATGTTT TCCTCGCACT TGACGTCGGT GACGTTTCTG
    GCTGATCCGA GTGCAGGCGG AGGCCTTCCC AGAGGAACCT TCATCTACGC CACTTCACCC
    GTGCCCATTC AGGCTCCGTC TTTTTACTGG GAGATCGAGA TCGTTTCGTT CGGAGACTCG
    GAGGACGACA GCGGTCCAAT AGTGTCCTTT GGATTTTCCC CGGAGGCGGA GAAGAGAGAC
    GGAGCCTGGA CCAATCCCGT CGGGACCTGC CTGTTTCACA ATAATGGCCG GGCGGTTCAC
    TACAACGGCT CCAGTTTACT GCAGTGGAAA AGTGTGAGGC TCGACGTGGC TCTGCTTCCT
    GGTGATGTAG CCGGCATCGG CTGGGAGCGT CTGGAAGGCA CTCCTCCTCC ACCCGGTCAG
    CCTCCCAAAG GAAGAGTGTA CTTCACCTAC TGCGGTCAGC GACTCGCTCC CTACCTAGAG
    GATGTGGCTG GAGGCATGTG GCCCGTCGTT CACATCCAGA AGAAGAATAC GAAGATTCAC
    GCCAACTTCG GCTGCCGGTT GTTTGCGTAC GCTGAAGGCC AGGCTCACAG AAACGCTGCT
    GATCTCTGCA TGGATCTGTC AGAGGAGATC AGTGCTAACT TCGAGGCTCT GCCCTTCGCC
    ATGGCCTCCG ACAGCGATAA TGATGCGGGC ACCAGTGTGG CGTCTGACTC CGGCTCCCAC
    GGGCCTCCTT GTCGCATCGC TGCCGTCGTC ACAGCACAAC AGCAATACAA CAGCGATGCT
    TCTTGCCACT ACAAGGTAGA GCTGAGCTAT GAGAATCTGG TGGTGTCGGG ACCGAACGCT
    CATCCGCCAC CCATCGCTGA TGACGAGAGC GATGATGAGG ATGATGAAGA CATTCCCCGG
    GAGGATCATT ACGCCCTGCT AGTGAAAGCG TGGGAAACTA AAGTGTTTCC CACCATTCGC
    CGGCGGTTCA GGAACGAGGC GGAGAGGAAA TCTGGCCTGG ATCAGATCAA GGGAGCGCTG
    CAGCTCGGTA TGGTGGACAT CGCTCGTCAG ACGGTTGAGT TTCTGTATGA AGAGAATGGC
    GGGATCCCTC GAGACCTCTA CCTTCCAACC ATCGAAGACA TTAAGGATGA AGCAAACAAG
    TTCACCATCG ACAAAGTCCG CAAAGGCTTG ACGGTGGGCA TCCGCTGTCC AGAGGGAAAC
    AACACCTGCA GCAGCACAGG AGGAACCGCT CTACCAAAGT TTGCCATCCG CGGCATGTTG
    AAGACCTTCA GCTTACATGG AGTTGTGCTC GATGTGGATT CGGGTAATGA GCTGGTGCAG
    GTGGAGACGT ATCTGCGCAG TGAGGGTGTG TTGGTGCGCT ACTGGTACCC CATAGAGATG
    CTGGAGAGAC CGCCGGCAGG ATCCCGCAGG GCTGCAGCCA ATGGCCTGGT GTCTTTGGAC
    AGCAGCAACA TCCAGATTCA TCGGGAGCTG CTGCGCTGTG AAAGTGCTCT GGCGCGTCTG
    TACTGCCGCA TGGCGCTGCT CAACATCTTT GCGCCCAAGA GTCCTCACAC ATTCACGCGC
    CTCTTCCACA TCCCTGCAGT GCGTGACATC ACACTCGAAC ACCTGCAGCT CCTGTCCAAT
    CAGCTGCTGG CTCCGCCTCT GCCAGACGGC CGGATCAGCT GTAACTCTGT GCTGCTGGCT
    CAGTCGGTCC TACATGAACT GCAGGGTCAG AGCTGCTCTC CCACTGAGCT CTTCTATCAG
    GGAAACGCTC AGAGTGTCCG CGAGTGGCTG AGCGTGGCCA TCAGCAGAGC TCTGCATCAG
    GGGGAGGACA GTCTGCTGGA GCTCACCAAA CAGATCTGCT GCTTCCTGCA GAGTGCGCCT
    GAGCAGTTCA CGCTGGAGGA GATTCCCATC ACCGAGTCAA AGGTCAGCAT GGACGTCAAC
    TTCCCCGGCG CTGCGTTTGT GCTCGTCTCC TGCAAAGAGA GTCAACTCAG CTGCCGCAAA
    GATTCCTCTC TGTACAAAGC TCCATGGGCT CGAGTGATGG TGTATGGGCT CGGTCATAAA
    GTGCGGCGTA ACGGCCAGCT GAACCTGATG GAGGCCGTGT GTTTCCCGCT GGACGCCTCT
    CCGTCCAACA CGGGCCTCAC TCCTCCACCC ACAGCCAACC AGTATCCCAG CATCATCATC
    CCCACCGACA GAGTGCACGT CAAACTGGGT GTGTCTCCCC CTCCTGGAGC AGTGCTGGTG
    TTGCACTCGT TGCCTCTTGA GTTTCCATTG GCGATGGCCT TCGCTGAACA GCTGCTAACC
    TACAGTGTAG GAGAGACCGT TGAGCGCTCT GAGGATGAGC TGGACACGGT GCCCACCTCT
    GTGCTCATGC AGGTCGTGGA GCTGCTAGGT GGCCTTTTGT GGACCACAGA TCTTGCTCCG
    AGCATCAAGG AGCTGACCTT CCACCTGCTT GCAGAGCTCC TCCGCAAGAT CCACGCTTTT
    GAGCGCCGCA AATCCCCTTC GGGTCTCTCT AGCCACATCA CGCTGCTTCT CAACCCCTGC
    CTAACAGTCC TGATGGTTCT TCAAACCGAA CTACGAAAGC TTTACGACCA CGAGACCCAA
    AGCTGGGGGT TGGTTAGCGG AGGATCCTGC ACTTCCGCAA CAGGCGGCAG TGGAGCGCTT
    GCACTTTCTG CAGAGCGGAG CCGCTTCTCT ACATACTTCC ACGCACTGAT GGAGGTTTGC
    CTGGCAGTGG CGGAAGTCAC TTTACCGCTG AGTTCTGGTT CTGGGACGTC AGTAATCGGC
    GTCCCTTCAT CAACAAGCAC AGCCAACTGC AGTGATTCCT CATCCTCTTC TTCGTCCTCT
    CCAGGCCAGA CGCCGCAAAG TCCGAGTTTG CTTTCAAAGC GCAAAAAGGT GAAGCTAAAA
    CGTGAGCGCG CTGCAGTCGG CTCAGCTTCC GCCGTCTCAG GGAAAAGTGG CTCGAGATCA
    TCAGAATGCG ACAATGCTCT ATTAAACATG AAGCCTGAAG ACATGTTGTG GTTTCACCGG
    GGGTTGACCT TGCTTTTGAT TTTACGGCAT CTTACTAATA AGGACCCGCA AGGTCTTGGC
    GTCACCGGCG ACGCTGTGGC AGACGCTTGC CAAGCTTTGG TTGGCTCGAC AGCTCACAGT
    CGCTTGCTGG TGCTCTCAGG AATCCCGACT CACCTGGAGG AGTCTGTGGT GCGCAATGCA
    ATACGAAGAG CATGCAATGC TCATGGCGGG CTCTTCAGGG ATGAGATCTT CATTCCAGTT
    CAGGAAGAAG ACCCTAAAAA AGCCAAAGCG GAAACGACAT GTGTTCCTCA AGATGTAAAG
    AGAGAATGCA CTGCAGGAAG CCCCAACAGC AACAGTGCGG CAATGAGTGT CTCCAGCGCT
    TTACAGGCAG CCTTCAGAGC CTCCGGAGAG CCCTTGTCCA ATCAGGAGAT GGTAAACAGT
    CCTTCCCAGC AACAAGCACC TCTGGATCCA CTCACAGTGT CCAGTCAGGA GAGTCTGGAC
    GTGTCTCTGT GTAGCACAGA AAGTCAAGGG AGTCTCGGCA GCCTCGGGGA GCAGGATAGT
    GCTGCCACGT CAGTCTCAGA TGGGAGCTCC ATGTACACGG TGGCCTCACC TGATGTTCAT
    GCCAGTGTGA CGCGTCCCAT AAAAGGCTAT GCTGTCATTG AGGTAAGGGC ACGAGCTCAG
    GTGGAGAAGA TTCGGGCCAG TCTTTTCAAC AGCAGCGATC TGATTGGCCT CTCCAATCTG
    GAAAGGGAGG AGGAGCTAAT GGAGATGACC AATGAGGAGA TCCTCACCGC CTCCAGTGTC
    AATCAGAGTT TATTTGACAC ACAGGGCAGC TCTGCTCTGG AGGAGTACTT CCTGGATAAA
    TCCATCAGAG GAGACAAGCT GGTTCCTGGA GCTCGTGACG TGCTGACAGA CATCTTTAAG
    AGCTGCATAT ACGCAGAGCA GGCGCTGAGC CCCGTTCCCG TGAAACCTGT CAAAGTCTCA
    GACATTTACC TCAGCAAAGA GCAGATCAAC TCTCAGTCTC CCGGAAACCT GCTACACGTG
    TTTTTCACAC ACGTTCGGCC TCCCAAAAAA GTGCTCGATG ACCAGCTCAC ACAGATTCTG
    CGGAAATACG GCACAGTAAA GCCCAATAAA AACAAGCACA GCAAAGCGGG CAAAGAGCAG
    CACCAAGGCA AAGTGGTGAG CACAAAAAGA CCCATCACCA AACCTCCGGC CAAAGAGAAG
    TCCATGCTGA ACAGCGTGCG AACTGTACTG AGTGAGAAAA AAACCGTCCT CAAGCCAAAA
    TCTCCAGAGA AGACCAAACC CGATGAAAAA GACCCCGAAA AATCTCCTGC CAAAAAACCC
    GAAGTGCCAG AGGAGAAGTT TTTGACGCTG GAGGGTTTCC ACAAGTTTAC TCTGGACAGA
    GCCAAGCAGG ATATCCGCAG TGTTTGGCGG GCGATCCTGG CCTGTGGATA TGACCTGCAC
    TTTGACAGAT GCACATGCAT TGATTCCCGA CATGCTCAGA AAGCCAGCAG AAAATGGAGC
    TTCGAGATGG ACTTTGCGTT GGTGCAGTTT GTGAACCGTC TCTGTCGTCA CCTGGCCATC
    ACTCCTGCCC GCCTGCATCC ACATGAGGTT TACCTGGACG CCAGCAAAGA TGGCGCGGAT
    CCTGAGGTCG CGTGTCTGTT AGGTGTCCCA GTGGAGAGTT TGCGTCTGCG CTTTGCTCTG
    CTGCAGTCCC TCAACAGCAC GCTGGAGAGC TTCTTCCTGC CTTTAGTGGA GCTGAGACTC
    ACACAGACCT ACCAGAACAG CATCGCCACA CTTCTGTGCG AGGCCAAAGG CCTGATATTT
    TACGACACTA AGGTGACGGT GATGAACAGG GTGTTGAACG CTACCGTGCA GCGGACGGCA
    GATCACGCAG CGCCTGAAAT CACTCTCGAC CCGCTGGAGA TTGTTGGAGG AGAGATGCGC
    GCTGCGGAGA ACACTTATTT CTGCCAGGCA GCGCGGCAGC TGTCGTGTGT GCCATCGTCT
    CAGCTGTGTG TGCGCATCGC CAGCGGAGGA GACCCCACAT ACGCCTTCAA CATCCGCTTC
    ACCGGAGAGG AGGTCCACGG CACCAGTGGC TCTTTCAGGC ATTTCCTATG GCAGGTGTGT
    AAGGAGCTCC AGAGTTCAGC TCTCTCTCTT CTTCTGCCCT GCCCGAGCGC CGCCGCTAAC
    CGCAATAAGG GGAAGTACAT CCTGACTCCG TGTCCCATCA CATACGCTGA GGAGCAGCTG
    CTGAACTTCT TCGGGCAGCT TCTGGGCATC GCCATCCGGG CCGATGTGCC GCTGCCTCTG
    GACCTGCTGG GATCCTTCTG GAAGGGTCTG GTCGGAGAGG CTCTCGATCC CGAAGCTGAC
    CTGCGGGAAG CAGATCTGCT CACGTACAGC TACATCAAGA AGTTTGAGAG TTTGTGTGAT
    GAAGCGGAGC TGGAAGCGCT GTGTGCTGAA GTGTCGTCTC AGAGTCCCCT GGGGGAGAGT
    CCAGACTCCC CCAGCCGGCC CTGCTGCACC TTCACATACA TCACTATGAC TGGAGAGGAA
    GTGGAGCTGT GCCAGGGAGG ACGTGACATC GCTGTCAGCT GGGAGAATAA GGATGTGTAT
    GTGCGAGCGG TGCGTGAGCT GCGTCTGCGG GAGCTGCAGA ATGTGGAGTG TATGTCTGCG
    GTGCGCGCCG GTCTGGGCTC CATCATCCCA CTGCAGCTGC TGACTTTACT GAGTCCCGCA
    GAGGTGGAGC TGCGCACCTG CGGTCTGCCG GACATCAACC TGGAGTTCCT GAAGGCGCAC
    ACCATGTATC AGGTGGGTCT GATGGAGACG GATCAGCATA TCGAGTTCTT CTGGGCGGCG
    CTGGAGATGT TCACGCAGGA GGAGCTCAGC AAGTTTATTA AGTTCGCCTG CAACCAGGAG
    AGGATTCCCT TCACCTGCCC GTGCAAAGAT GGGGGTCCGG ACACCGCACA TGTGCCCCCC
    TATCCCATGA AGATCGCCCC GCCGGACGGC ACCGTGGGCT CTCCAGATCA GCGCTATATC
    CGCGTGGAGA CGTGTATGTT CATGGTCAAG CTGCCGCAGT ATTCCTCCCT GGATGTGATG
    CTGGAGAAAC TGCGATACGC TATTCACTAT CGAGAAGATC CTCTGAGCGG ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa51540
    Clone ID Related Accession (Same CDS sequence) XM_009303669.3 , XM_009303669.2
    Accession Version XM_009303669.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 13254bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product probable E3 ubiquitin-protein ligase HECTD4 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007118.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009303669.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

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    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
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    2161
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    2641
    2701
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    10261
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    10501
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    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGCTCAG GTGCTGTGGA TTCTTCCCAG TGGCTGTCAG TGAAGGAAGA GACTATTTTC 
    CTTCACGATG GACTAATTCG GGTCACGGAC CTGGCTGAGC TGCCCAGCGA AATCGGCGTG
    TCTGAACAAG CTGAATCCGA GCAGGAGATC TTGACGTTTG AGACCAAGAA TCCAGTGGAG
    CTAGCCGAGC GTCTCCGAGC CGTCTGTGGA AACCAGAGCA ACGCTTATGC ACGCCTTTTG
    GAGTATCGTC TGAATGCACT GCGTGGCCTG TGGGGGGCGC AGCGTCAGCT GGCCCTGGAG
    GAGCAGCAGG ATCATGACGG GGTTGTGTCA GCCAGCGGCG GAGGGTCCGA CGAGGAGACA
    CTGGCCCTAC TCAAACGACA GGGTTTACTG CAACCCCAAC ACCACCACCA TCCACACCAC
    CCTCCGATGG CCAGCGGCTC AGAGCAGGCA CCATTCACCT CCCGTGTGGG CCTGCTGTTG
    GTTTTCCCAC TGCTGCAGTC GCAAACTCGG CACGACCCTG CGCTGTGCGG CGTCACAGCG
    GAGGTGTTGC TCGCATGTCT GCGGGACTGC CAGCCACTCA GCCTGGGGAA GGAGCCCGCC
    GACTGTCTTA ATGGCCTGGA GAGGTTGCTG TGCTCCTGGT TGGAGGAGAC GGAGGGTTCG
    GCACCAATGA GGCCACTGCT CACCCAACAG CAGAGACAGA ACGCAGCTGC GGCGCTAGTG
    GCTCTGGCAT GTGCAAGGGG CTCCCTCAAA ACCTTCATCC ACACGGTCCA CCTGCTGCAG
    AAACAAACAG ACCTGGGTCA GCTTCCTGTG GCTGATGTTC TCTATCGACT GCTGCTTCTT
    GAAGGAGGTC CAGGTTCGCC CTCATGTCTG CTGGGGGGGA AACATATTGT GTCGTGGGGC
    TTTGAGGACA TGCTGCCCAC ACCAGAGAGC AGCGCCGCAG GAGCCGACAG TAAAGACACA
    GATCTGGGCC GCAGCCTGGC CACAGATGGA TTCTATCTCT ACACCACGAG CTCTTTTGGG
    AAGGGTTTGA GCAAGCTGGG CTCCGGGCAG CACGGGACGC TGAGGGGTTT TGTGTACTGT
    CGTAATGAGG AGATGGAGGC GGGCTGGGTG GCTCACAGCA GTGGATTTCT GCTCCACCGT
    CCTGCATCAT TTGACACTAA ACCTCATCAG CTGTGCCACG TCATCGACCC GTTCACACTG
    CAGGTTTCTC AGGTGTTAGC GATGCCCGTT CATCATTTCC CAGTGGGCAG CTGCCTGACC
    ACGCTGCGTC TGTGTTCTGA CGGCACATAC TTGTATTGGG TTTGGTCTCC GGTCAGCATC
    AACGAGAAAA CGCAGAAAGG CCACTCTGTC TTCATGGACG TCTTCCAGTT AACGAGTGAG
    TCCGGTGTGT GTGTGGCCAG TCCTGTACAA GACCGGGTGA TCCTGTTGCG AAAGGAAGGC
    GAGTCGTCCA AGTGCTTGAA TGAGTTGCTC CTCAGCCGAA TGTCGCGCTT CCGAGCCTCG
    CACTCGTCCA CACTGGCGGC TCTCACCGGC TCCGCCATCA CCAACCCTGT CAAAGAGGAG
    CAATCGGTGG TCAACACAAG CTGTGGTCTG TCCATCAAAA CACTGAGGAA GACACCCATG
    TATGTGTGCG GGACGTCCCT GGTGATGCTG GTGCCGACTC CAGGTGTTGC TGGGAGCTCC
    GCCACGCGCT CGCTGTTCGG GGGATCCAGT GGCCTTTCTT CTCTCAAGAT CTTGGCCTCT
    AGTTTGGTGT TTAACCTGGC GGACGGTCAG TTCAGCAATC GCGTGGATCT GGTCGACGCT
    CCGGGAAGTT CTCTGGGTCG TGGAGCTCAG GTTGCAGGAC TCGGCGCCTG TTATGATGCA
    CTGAACAACT TAATCTGGAC GTGTTCGAGC GACTACATGG ACCAGTGGTG CAACCCTGGA
    AATCAGGCCT TCCATCATGT GTGCCAGCGA CTCGGCGTCA GTCACGCCAT CAGCCAGACC
    AAAGAAGAGA TGATCGACAC ATCCAGCGTG ATCAGCCAGC TGCTCCATCA TGTGGGAGCC
    ATGTGTATCC ATCAGCTGAA CCTGCTGGCC GCCAGCAGTA GTTTACACTT GGGCACCTTC
    ATAGGGAAGC AGCCAATGGA GGCCTGTCAC TTCAGCTCCA TCTGTGACGT GATGGAGAAA
    GCCATGGTCA ACGGAGACAC CCGCATCATC CGCTGCATCC TGGTCGTTTT TCAGGTGGTT
    TTCCAGTTTT TTAATCCAAA CTCAGAGCAG AACCGGGAGT GTGTGAAGCG CTCAGGACTG
    CTGCTGTGGC AGTTACTCAT GGCTCCACCT GATCAGATCC AGCCGGAAAT ACAGAAAGAA
    GTTTGTCTCG CCATCAGCTC AGGTTTAAAC ACCCTGTATC AGAGCGAATC TGAGTTGAAC
    AACCTGCTGA AGTTTGTTTT CACAGAGGGG GAGAAGAACA GTGGTTTGTC TCAGTTACGA
    GACGTGATTT TAAACAATCT GGCTAACCAG TTACAGAAGA ACCGCTTTGG TAGTGAAGAT
    GATGATCACT ACAGGCTGAA GGATGAGCTG CTGCAGTGCA TCTTGAAGAC GGTGGTGCGC
    GAGTCGTGTC TCCTCATCAC CAAATGTCAA ACCGTTTCCA AAGAAGACCT TCAGAAGCTG
    CTCTCTACTG TACCTGTGGT GTCTCCCAGC TTGCGTTATT TAATGGCTGT GCAGAATCAT
    CTGCTCAGCA ACACCATCCT GCTGCACCCG GAGGACATGG AGGACAGCGA CAGCTCCCTG
    CAGGGGGAAA CAATGAAGGA GCTGCAGACG AGCATCTTGT CTCTTGCGTT GAAGATTCTG
    GTTGGATGTG ATGAAGTTCT GGAGACGCTT CAGCAGGTGA CCAGCTCTCT CATCAACAGC
    AGCATCAGCG ACCGAGAGAC CAGGCTCAGA GGTCTTGAAC AGGTGACCAA GGCCACCATG
    TTGGGTCATT TGCTGCCGGT TCTGCTGACG TCACTGATGC ACCCGAACCT CCAAACACTC
    ACGCTAGCAG ATGCGCTCAT GCCACAGCTA GTCCAGCTCG TCCTCTACAC TAGCCAGACC
    GCTTTGCTGC TAAAGACACA TTCTCCATTA TTTGTTGAGG ATTCCCCAAT AAATCAAGGC
    TCCAGCCAGC CCTCATTAAA TGACAGTTTT CAGATTCTGG AGGAGAGAGA GGAGCCTGGC
    TTTCTGACGG GGCTGAAGAT CCCCGCCCCG TGGGCTGCTG GGAAAACGGT GGAAACTGTT
    CACCCGGTGC GGGATAACTA TAAATTTAAA GACACAGTGC ACATTCCTGG AGCTCGCTGT
    CTGTACCTGC GCTTCGACCC GCGCTGCTCC TCACAGTACG ACTACGACAA GCTTGTGATT
    TACGCCGGGC CGAACACAAA CAGTCGTAAA GTAAGCGAGT ATGGAGGAAA CACCATGGGT
    TATGGCAGCC GCAGTGTATT GGGAACCGGA TGGCCAAAAG ATCTGGTCAA GGTGGAAGGA
    GATACGGTGA CGTTCTCTTT TGAGATGAGA AGTGGACGCG AGCACAACAC TCCAGATAAA
    GCCATGTGGG GTTTCTCGTG CACCGTCAGA GCGCAGGAGT CGTCTGAGGA TGTGTCTGGC
    GGTCTGCCGT TTCTGGCTGA TCTGGCTCTG GGTTTATCAG TGTTAGCCTG CGCTATGCTA
    CGAATCCTCT ATAATGGGCC TGAAATCACA CGAGAGGAAG AAGCTTGTCA CGACCTTCTC
    TGCTCCAAAT TACTGCAGAG GTGTCAGTGG CAGGTGGAGG CGAACGGAGC CATCTCTCCT
    GCACTCACGC CATCTCCGTC TCCTCTTCCT CTGACTATCG ATGAGGAGCG CGAGTTCACA
    TATCCATCAG ATATCCTCGC TCCGCCGCCG GGGCCCATCC CGAGCGCCTG CTGTGAGCTG
    CCCCGCATTC ACTTACCGCA GGGTATCATG GGCCGCCTGA GGGAGCTGTC TGGACGTGCT
    CGGCCACAGT TTCGCCCCAG CATCAAAGAG GTGATCCGTC CAGACGTGAT GGAGGAAGTG
    ATTGTGTCGT GTGTGATTAA ACACTTGGGT CTGGTGGACG CGCTGCAGTC GCTGGTCAAC
    TTCCAGTACA GAGAATCTCA CAGAGAAGAA CACAACCTGC TCTGCAAGAT CATGGCTGAG
    ACCTTCAAGA AGATCAACGC CATGGAGAGA CAGTTACAGA GCGTGGCTGA GCTGGAGCAG
    AAGTGGCAGA ATGAAGTAGA TGAAGCTCAG CAGGGAAAAC TGGAGAACAA CACTCCTTTC
    TTCCACGATT ATCACTTCTT TGAGAATAAA ATGAAGGAGC TGGAGCTTCT CTGTTCGCTA
    AAGGAGGTTC CTCTGGACTG CACTGATCCA GAAAACGCTG TGATGGCTTT GAGAGAGAAG
    TTTTTTCATG AGGTGAACTC CACGCTGCAG CAGCAGAGAA GTGTTGTTCC TCTGGCTAAA
    ACCAAAGCGC TTGTGAAGAG TTTGATCAAC CGCTCGGAGC TGCTGCTTCA TGTCACCATC
    GCTCCGCAGT GCAAGAGTCT GTCATCAACA CCCACAAGCA CACCGGCCTG TAAGTCGGTG
    TCAGACGCTA AATCGATGGC TCCCGTGGTG AAGCAGCCGG TGTTCCTGCG CAGCATGTCT
    GCTCCGTCAG ATCTGGAAAT GATCGCCAGC AGTGATGTGG AGTTCACACA CGGAGCGCAA
    AGGAGGCGGC AGCACCCCTC CAGCCACCGG AGCAGCTCAT TTACACTCCT GCAGTCATTG
    GTGATTGAAG ACAGTAAAGA TAAACCCACG TACAGTGTGC TGCTGGGACA ACTGTTCGCC
    TTTATAGGAA CCACTCCTGA CCAAACCGTG TCGAGCAGCA GTTTTCTGTC CGCAGCTCAG
    ACGCGCTGGA GAAGAGGACG AACACGCAAA CAGGCTCTGG TTCACATGCG GGAGCTGCTG
    ACCGCTGCCG TCCGCGTCGG TGGAGTCACT CATCTGGTCG GACCCGTCAC TATGGTCCTG
    CAGGGGGGAC CCAGGGTGGA GGAGCTCACC TGTGGTGGGA TGATGGAGCA GGTGCAGGAG
    GCGTTTGGAG AGACCATGAC GTCAGTGGTG TCTCTGTGTG CCCGATACCC CATCGCCTGT
    GCCAACAGCA TCGGCCTGCT CTGCACCATC CCCTACACCA GGAGTGAGGA GCAGTGTCTG
    GTGCGCAGCG GTCTGGTTCA GCTCATGGAT CGCCTCTGCA GTCTGAGCAG TCAGAGAGAC
    TGCAGCTCCA ACGAGAAGCA AACCAAAAAG CAGAAAGTGG CCACCATGGC CTGGGCTGCG
    TTTCAGGTGC TGGCCAACCG CTGCATTGAG TGGGAGAAAG TCGAAGGAGG CTCCACAGAC
    GCAGTGCATT CAGGGTTGGC TCGACAGGTG TCCAGCCTTC TGACCAATCA TCTGGCTCGA
    GCGACAGACT GCTGTGGAAA CCAGGCGGCA GGAAATGATG CGCTGCAGGA TGTGCTCAGT
    TTACTCAACG ATCTGTCCAG GAGTCACATA GGCAAGACCA TCCTGAGCCA GCCTGCCTGT
    GTGTCCAAGC TGCTGTCTCT GCTTCTGGAC CAGCGACCGT CGCCCAAACT GGTGCTGATC
    ATCCTGCAGC TGTGCCGCGC CGCGCTGCCC CTCATGAGTG TGGAGGATTG TGGAAATGTA
    GCGCTGCCGT CCTGGAGCTA CTCCACAAAC GCACTGGACG CCGAGCAGCA GGACGCCAGC
    GACCCTGCCT TCCGCATCGC ATCTCTGCTG CTCGCCAAAC TGGCCGACTA TGTGGTGCCA
    GGCTGTCAGA CGTTGCTCTC TCCCAGCGCC TCTGAGCCGG ACACCAGTCT GAGCCGAGCC
    TGTCCCAAGA GCAGCTCTAA GACCGACAGA GATGCCAGCG AGGAGGGCGA AGCGGTGGAC
    GGAAAACTCT CCATATTCAT CCATAAGAGA GAAGACCAGT CGTCGCACGA GGTGCTGCAG
    CCTCTGCTCA GCAGCTCAGA GGGTCGACCG TTTCGTCTCG GCACTGGAGC CAACATGGAG
    AAGGTGGTGA AGATGGATCG AGACATGACC AAGAGCGGCT GCTGTGAGGT CATCACTGAA
    GAGGCGTCCG CTGCTCTCAG GAAAGCCAAT AAATGGGCTC AGTCGGGGCT CATTGTGAGT
    GTGGGTCCGC CCATCGAAAG CAGCGCTCAG GAGAACTCAG GAGCGACCTC TACAGGAGAC
    AAGAAGAAAA GCGCTCAGTC CACGGTGTGT CGAGAGAGGA ACGCTGAGCT CGCCCGGTCT
    GACCCGGTGC GTCCGTTCAT CAGTGGTCAC GTAGCCAACA GTATGGCAGC AGAGGTCATC
    GCTCTGCTGC ACAGCCTCCT GACGGCCCCC GAGTCCAACA CTGCTCAGAT CTGGACAAGC
    ACTGCAGAAA AGGTGTTGTC CAGAGCTCTG ATGTTCATCC CTCAGCTGGG GAAGTTTGCT
    GAGAGTATTC TGGAGAGCGG AAACAGCAGC GGCAGGAAGT TGTCCAAGCT GCAGGCCATT
    GGTCGACAGG CGGTGGCGGC TCTCTGTGCG CTCGGCGGAT TCAAGGAGAC CATCAAGATT
    GGATCTGAAG TGCAGGTGGT TGGTAAAGGT GTGTTGGACA GTGTCGGTGT GGTTATATCC
    ATTAATGAGC AGGAGGGCAT CGCCACCGTC AAGTTTCCAT CCTGCGAGTA TCGGCGCTCC
    AGCAAAGCAT CTGATATCCT GACGGTGCCC ATATCACGCC TGTGCACGCC GCGCTCAGAG
    GCTCTGCCGC TGCACAAGCT TTCCATCACA GAGAAGGTTG TTCAGGCCGT TCAGTCCATG
    TTGCTGCCGC AGGAGGGCAG TCTGTCCATA CACACGTCTC TGCCGGCGTC TGGAGACGGC
    TCCAGTCCTG TGATGGCAGT GGTGCGTCTG CTGGCCGAGA TCAGAACCAG AGCGTGTCTG
    GTGATGGCGC AGCTGCTGGA GGACAGCTCT TTCTGTGAGG AGTTTATCCA GCAGTGTCCT
    GCAGCCGTGG AGGTGCTCAA CCTCATCGCT CAAGAGTGCA GTCCTGGAGA GCGTTTGGTG
    ATGGTGGAGG CTCAGTGTGA GCGTCTGCGC ATGCTTTATC GAGACTGTGC TCGTCCTCCA
    CCACCGCCTG TGCAAACTGA CCAACGGCAG CCGAAGGAGA TCACGTGGTG CCCGTCTCGA
    GTCTTCCCTC CGGTTCGGGC CTGTATGTTT TCCTCGCACT TGACGTCGGT GACGTTTCTG
    GCTGATCCGA GTGCAGGCGG AGGCCTTCCC AGAGGAACCT TCATCTACGC CACTTCACCC
    GTGCCCATTC AGGCTCCGTC TTTTTACTGG GAGATCGAGA TCGTTTCGTT CGGAGACTCG
    GAGGACGACA GCGGTCCAAT AGTGTCCTTT GGATTTTCCC CGGAGGCGGA GAAGAGAGAC
    GGAGCCTGGA CCAATCCCGT CGGGACCTGC CTGTTTCACA ATAATGGCCG GGCGGTTCAC
    TACAACGGCT CCAGTTTACT GCAGTGGAAA AGTGTGAGGC TCGACGTGGC TCTGCTTCCT
    GGTGATGTAG CCGGCATCGG CTGGGAGCGT CTGGAAGGCA CTCCTCCTCC ACCCGGTCAG
    CCTCCCAAAG GAAGAGTGTA CTTCACCTAC TGCGGTCAGC GACTCGCTCC CTACCTAGAG
    GATGTGGCTG GAGGCATGTG GCCCGTCGTT CACATCCAGA AGAAGAATAC GAAGATTCAC
    GCCAACTTCG GCTGCCGGTT GTTTGCGTAC GCTGAAGGCC AGGCTCACAG AAACGCTGCT
    GATCTCTGCA TGGATCTGTC AGAGGAGATC AGTGCTAACT TCGAGGCTCT GCCCTTCGCC
    ATGGCCTCCG ACAGCGATAA TGATGCGGGC ACCAGTGTGG CGTCTGACTC CGGCTCCCAC
    GGGCCTCCTT GTCGCATCGC TGCCGTCGTC ACAGCACAAC AGCAATACAA CAGCGATGCT
    TCTTGCCACT ACAAGGTAGA GCTGAGCTAT GAGAATCTGG TGGTGTCGGG ACCGAACGCT
    CATCCGCCAC CCATCGCTGA TGACGAGAGC GATGATGAGG ATGATGAAGA CATTCCCCGG
    GAGGATCATT ACGCCCTGCT AGTGAAAGCG TGGGAAACTA AAGTGTTTCC CACCATTCGC
    CGGCGGTTCA GGAACGAGGC GGAGAGGAAA TCTGGCCTGG ATCAGATCAA GGGAGCGCTG
    CAGCTCGGTA TGGTGGACAT CGCTCGTCAG ACGGTTGAGT TTCTGTATGA AGAGAATGGC
    GGGATCCCTC GAGACCTCTA CCTTCCAACC ATCGAAGACA TTAAGGATGA AGCAAACAAG
    TTCACCATCG ACAAAGTCCG CAAAGGCTTG ACGGTGGGCA TCCGCTGTCC AGAGGGAAAC
    AACACCTGCA GCAGCACAGG AGGAACCGCT CTACCAAAGT TTGCCATCCG CGGCATGTTG
    AAGACCTTCA GCTTACATGG AGTTGTGCTC GATGTGGATT CGGGTAATGA GCTGGTGCAG
    GTGGAGACGT ATCTGCGCAG TGAGGGTGTG TTGGTGCGCT ACTGGTACCC CATAGAGATG
    CTGGAGAGAC CGCCGGCAGG ATCCCGCAGG GCTGCAGCCA ATGGCCTGGT GTCTTTGGAC
    AGCAGCAACA TCCAGATTCA TCGGGAGCTG CTGCGCTGTG AAAGTGCTCT GGCGCGTCTG
    TACTGCCGCA TGGCGCTGCT CAACATCTTT GCGCCCAAGA GTCCTCACAC ATTCACGCGC
    CTCTTCCACA TCCCTGCAGT GCGTGACATC ACACTCGAAC ACCTGCAGCT CCTGTCCAAT
    CAGCTGCTGG CTCCGCCTCT GCCAGACGGC CGGATCAGCT GTAACTCTGT GCTGCTGGCT
    CAGTCGGTCC TACATGAACT GCAGGGTCAG AGCTGCTCTC CCACTGAGCT CTTCTATCAG
    GGAAACGCTC AGAGTGTCCG CGAGTGGCTG AGCGTGGCCA TCAGCAGAGC TCTGCATCAG
    GGGGAGGACA GTCTGCTGGA GCTCACCAAA CAGATCTGCT GCTTCCTGCA GAGTGCGCCT
    GAGCAGTTCA CGCTGGAGGA GATTCCCATC ACCGAGTCAA AGGTCAGCAT GGACGTCAAC
    TTCCCCGGCG CTGCGTTTGT GCTCGTCTCC TGCAAAGAGA GTCAACTCAG CTGCCGCAAA
    GATTCCTCTC TGTACAAAGC TCCATGGGCT CGAGTGATGG TGTATGGGCT CGGTCATAAA
    GTGCGGCGTA ACGGCCAGCT GAACCTGATG GAGGCCGTGT GTTTCCCGCT GGACGCCTCT
    CCGTCCAACA CGGGCCTCAC TCCTCCACCC ACAGCCAACC AGTATCCCAG CATCATCATC
    CCCACCGACA GAGTGCACGT CAAACTGGGT GTGTCTCCCC CTCCTGGAGC AGTGCTGGTG
    TTGCACTCGT TGCCTCTTGA GTTTCCATTG GCGATGGCCT TCGCTGAACA GCTGCTAACC
    TACAGTGTAG GAGAGACCGT TGAGCGCTCT GAGGATGAGC TGGACACGGT GCCCACCTCT
    GTGCTCATGC AGGTCGTGGA GCTGCTAGGT GGCCTTTTGT GGACCACAGA TCTTGCTCCG
    AGCATCAAGG AGCTGACCTT CCACCTGCTT GCAGAGCTCC TCCGCAAGAT CCACGCTTTT
    GAGCGCCGCA AATCCCCTTC GGGTCTCTCT AGCCACATCA CGCTGCTTCT CAACCCCTGC
    CTAACAGTCC TGATGGTTCT TCAAACCGAA CTACGAAAGC TTTACGACCA CGAGACCCAA
    AGCTGGGGGT TGGTTAGCGG AGGATCCTGC ACTTCCGCAA CAGGCGGCAG TGGAGCGCTT
    GCACTTTCTG CAGAGCGGAG CCGCTTCTCT ACATACTTCC ACGCACTGAT GGAGGTTTGC
    CTGGCAGTGG CGGAAGTCAC TTTACCGCTG AGTTCTGGTT CTGGGACGTC AGTAATCGGC
    GTCCCTTCAT CAACAAGCAC AGCCAACTGC AGTGATTCCT CATCCTCTTC TTCGTCCTCT
    CCAGGCCAGA CGCCGCAAAG TCCGAGTTTG CTTTCAAAGC GCAAAAAGGT GAAGCTAAAA
    CGTGAGCGCG CTGCAGTCGG CTCAGCTTCC GCCGTCTCAG GGAAAAGTGG CTCGAGATCA
    TCAGAATGCG ACAATGCTCT ATTAAACATG AAGCCTGAAG ACATGTTGTG GTTTCACCGG
    GGGTTGACCT TGCTTTTGAT TTTACGGCAT CTTACTAATA AGGACCCGCA AGGTCTTGGC
    GTCACCGGCG ACGCTGTGGC AGACGCTTGC CAAGCTTTGG TTGGCTCGAC AGCTCACAGT
    CGCTTGCTGG TGCTCTCAGG AATCCCGACT CACCTGGAGG AGTCTGTGGT GCGCAATGCA
    ATACGAAGAG CATGCAATGC TCATGGCGGG CTCTTCAGGG ATGAGATCTT CATTCCAGTT
    CAGGAAGAAG ACCCTAAAAA AGCCAAAGCG GAAACGACAT GTGTTCCTCA AGATGTAAAG
    AGAGAATGCA CTGCAGGAAG CCCCAACAGC AACAGTGCGG CAATGAGTGT CTCCAGCGCT
    TTACAGGCAG CCTTCAGAGC CTCCGGAGAG CCCTTGTCCA ATCAGGAGAT GGTAAACAGT
    CCTTCCCAGC AACAAGCACC TCTGGATCCA CTCACAGTGT CCAGTCAGGA GAGTCTGGAC
    GTGTCTCTGT GTAGCACAGA AAGTCAAGGG AGTCTCGGCA GCCTCGGGGA GCAGGATAGT
    GCTGCCACGT CAGTCTCAGA TGGGAGCTCC ATGTACACGG TGGCCTCACC TGATGTTCAT
    GCCAGTGTGA CGCGTCCCAT AAAAGGCTAT GCTGTCATTG AGGTAAGGGC ACGAGCTCAG
    GTGGAGAAGA TTCGGGCCAG TCTTTTCAAC AGCAGCGATC TGATTGGCCT CTCCAATCTG
    GAAAGGGAGG AGGAGCTAAT GGAGATGACC AATGAGGAGA TCCTCACCGC CTCCAGTGTC
    AATCAGAGTT TATTTGACAC ACAGGGCAGC TCTGCTCTGG AGGAGTACTT CCTGGATAAA
    TCCATCAGAG GAGACAAGCT GGTTCCTGGA GCTCGTGACG TGCTGACAGA CATCTTTAAG
    AGCTGCATAT ACGCAGAGCA GGCGCTGAGC CCCGTTCCCG TGAAACCTGT CAAAGTCTCA
    GACATTTACC TCAGCAAAGA GCAGATCAAC TCTCAGTCTC CCGGAAACCT GCTACACGTG
    TTTTTCACAC ACGTTCGGCC TCCCAAAAAA GTGCTCGATG ACCAGCTCAC ACAGATTCTG
    CGGAAATACG GCACAGTAAA GCCCAATAAA AACAAGCACA GCAAAGCGGG CAAAGAGCAG
    CACCAAGGCA AAGTGGTGAG CACAAAAAGA CCCATCACCA AACCTCCGGC CAAAGAGAAG
    TCCATGCTGA ACAGCGTGCG AACTGTACTG AGTGAGAAAA AAACCGTCCT CAAGCCAAAA
    TCTCCAGAGA AGACCAAACC CGATGAAAAA GACCCCGAAA AATCTCCTGC CAAAAAACCC
    GAAGTGCCAG AGGAGAAGTT TTTGACGCTG GAGGGTTTCC ACAAGTTTAC TCTGGACAGA
    GCCAAGCAGG ATATCCGCAG TGTTTGGCGG GCGATCCTGG CCTGTGGATA TGACCTGCAC
    TTTGACAGAT GCACATGCAT TGATTCCCGA CATGCTCAGA AAGCCAGCAG AAAATGGAGC
    TTCGAGATGG ACTTTGCGTT GGTGCAGTTT GTGAACCGTC TCTGTCGTCA CCTGGCCATC
    ACTCCTGCCC GCCTGCATCC ACATGAGGTT TACCTGGACG CCAGCAAAGA TGGCGCGGAT
    CCTGAGGTCG CGTGTCTGTT AGGTGTCCCA GTGGAGAGTT TGCGTCTGCG CTTTGCTCTG
    CTGCAGTCCC TCAACAGCAC GCTGGAGAGC TTCTTCCTGC CTTTAGTGGA GCTGAGACTC
    ACACAGACCT ACCAGAACAG CATCGCCACA CTTCTGTGCG AGGCCAAAGG CCTGATATTT
    TACGACACTA AGGTGACGGT GATGAACAGG GTGTTGAACG CTACCGTGCA GCGGACGGCA
    GATCACGCAG CGCCTGAAAT CACTCTCGAC CCGCTGGAGA TTGTTGGAGG AGAGATGCGC
    GCTGCGGAGA ACACTTATTT CTGCCAGGCA GCGCGGCAGC TGTCGTGTGT GCCATCGTCT
    CAGCTGTGTG TGCGCATCGC CAGCGGAGGA GACCCCACAT ACGCCTTCAA CATCCGCTTC
    ACCGGAGAGG AGGTCCACGG CACCAGTGGC TCTTTCAGGC ATTTCCTATG GCAGGTGTGT
    AAGGAGCTCC AGAGTTCAGC TCTCTCTCTT CTTCTGCCCT GCCCGAGCGC CGCCGCTAAC
    CGCAATAAGG GGAAGTACAT CCTGACTCCG TGTCCCATCA CATACGCTGA GGAGCAGCTG
    CTGAACTTCT TCGGGCAGCT TCTGGGCATC GCCATCCGGG CCGATGTGCC GCTGCCTCTG
    GACCTGCTGG GATCCTTCTG GAAGGGTCTG GTCGGAGAGG CTCTCGATCC CGAAGCTGAC
    CTGCGGGAAG CAGATCTGCT CACGTACAGC TACATCAAGA AGTTTGAGAG TTTGTGTGAT
    GAAGCGGAGC TGGAAGCGCT GTGTGCTGAA GTGTCGTCTC AGAGTCCCCT GGGGGAGAGT
    CCAGACTCCC CCAGCCGGCC CTGCTGCACC TTCACATACA TCACTATGAC TGGAGAGGAA
    GTGGAGCTGT GCCAGGGAGG ACGTGACATC GCTGTCAGCT GGGAGAATAA GGATGTGTAT
    GTGCGAGCGG TGCGTGAGCT GCGTCTGCGG GAGCTGCAGA ATGTGGAGTG TATGTCTGCG
    GTGCGCGCCG GTCTGGGCTC CATCATCCCA CTGCAGCTGC TGACTTTACT GAGTCCCGCA
    GAGGTGGAGC TGCGCACCTG CGGTCTGCCG GACATCAACC TGGAGTTCCT GAAGGCGCAC
    ACCATGTATC AGGTGGGTCT GATGGAGACG GATCAGCATA TCGAGTTCTT CTGGGCGGCG
    CTGGAGATGT TCACGCAGGA GGAGCTCAGC AAGTTTATTA AGTTCGCCTG CAACCAGGAG
    AGGATTCCCT TCACCTGCCC GTGCAAAGAT GGGGGTCCGG ACACCGCACA TGTGCCCCCC
    TATCCCATGA AGATCGCCCC GCCGGACGGC ACCGTGGGCT CTCCAGATCA GCGCTATATC
    CGCGTGGAGA CGTGTATGTT CATGGTCAAG CTGCCGCAGT ATTCCTCCCT GGATGTGATG
    CTGGAGAAAC TGCGATACGC TATTCACTAT CGAGAAGATC CTCTGAGCGG ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009301944.1
    CDS430..13683
    Translation

    Target ORF information:

    RefSeq Version XM_009303669.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio HECT domain E3 ubiquitin protein ligase 4 (hectd4), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009303669.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    4681
    4741
    4801
    4861
    4921
    4981
    5041
    5101
    5161
    5221
    5281
    5341
    5401
    5461
    5521
    5581
    5641
    5701
    5761
    5821
    5881
    5941
    6001
    6061
    6121
    6181
    6241
    6301
    6361
    6421
    6481
    6541
    6601
    6661
    6721
    6781
    6841
    6901
    6961
    7021
    7081
    7141
    7201
    7261
    7321
    7381
    7441
    7501
    7561
    7621
    7681
    7741
    7801
    7861
    7921
    7981
    8041
    8101
    8161
    8221
    8281
    8341
    8401
    8461
    8521
    8581
    8641
    8701
    8761
    8821
    8881
    8941
    9001
    9061
    9121
    9181
    9241
    9301
    9361
    9421
    9481
    9541
    9601
    9661
    9721
    9781
    9841
    9901
    9961
    10021
    10081
    10141
    10201
    10261
    10321
    10381
    10441
    10501
    10561
    10621
    10681
    10741
    10801
    10861
    10921
    10981
    11041
    11101
    11161
    11221
    11281
    11341
    11401
    11461
    11521
    11581
    11641
    11701
    11761
    11821
    11881
    11941
    12001
    12061
    12121
    12181
    12241
    12301
    12361
    12421
    12481
    12541
    12601
    12661
    12721
    12781
    12841
    12901
    12961
    13021
    13081
    13141
    13201
    ATGGGCTCAG GTGCTGTGGA TTCTTCCCAG TGGCTGTCAG TGAAGGAAGA GACTATTTTC 
    CTTCACGATG GACTAATTCG GGTCACGGAC CTGGCTGAGC TGCCCAGCGA AATCGGCGTG
    TCTGAACAAG CTGAATCCGA GCAGGAGATC TTGACGTTTG AGACCAAGAA TCCAGTGGAG
    CTAGCCGAGC GTCTCCGAGC CGTCTGTGGA AACCAGAGCA ACGCTTATGC ACGCCTTTTG
    GAGTATCGTC TGAATGCACT GCGTGGCCTG TGGGGGGCGC AGCGTCAGCT GGCCCTGGAG
    GAGCAGCAGG ATCATGACGG GGTTGTGTCA GCCAGCGGCG GAGGGTCCGA CGAGGAGACA
    CTGGCCCTAC TCAAACGACA GGGTTTACTG CAACCCCAAC ACCACCACCA TCCACACCAC
    CCTCCGATGG CCAGCGGCTC AGAGCAGGCA CCATTCACCT CCCGTGTGGG CCTGCTGTTG
    GTTTTCCCAC TGCTGCAGTC GCAAACTCGG CACGACCCTG CGCTGTGCGG CGTCACAGCG
    GAGGTGTTGC TCGCATGTCT GCGGGACTGC CAGCCACTCA GCCTGGGGAA GGAGCCCGCC
    GACTGTCTTA ATGGCCTGGA GAGGTTGCTG TGCTCCTGGT TGGAGGAGAC GGAGGGTTCG
    GCACCAATGA GGCCACTGCT CACCCAACAG CAGAGACAGA ACGCAGCTGC GGCGCTAGTG
    GCTCTGGCAT GTGCAAGGGG CTCCCTCAAA ACCTTCATCC ACACGGTCCA CCTGCTGCAG
    AAACAAACAG ACCTGGGTCA GCTTCCTGTG GCTGATGTTC TCTATCGACT GCTGCTTCTT
    GAAGGAGGTC CAGGTTCGCC CTCATGTCTG CTGGGGGGGA AACATATTGT GTCGTGGGGC
    TTTGAGGACA TGCTGCCCAC ACCAGAGAGC AGCGCCGCAG GAGCCGACAG TAAAGACACA
    GATCTGGGCC GCAGCCTGGC CACAGATGGA TTCTATCTCT ACACCACGAG CTCTTTTGGG
    AAGGGTTTGA GCAAGCTGGG CTCCGGGCAG CACGGGACGC TGAGGGGTTT TGTGTACTGT
    CGTAATGAGG AGATGGAGGC GGGCTGGGTG GCTCACAGCA GTGGATTTCT GCTCCACCGT
    CCTGCATCAT TTGACACTAA ACCTCATCAG CTGTGCCACG TCATCGACCC GTTCACACTG
    CAGGTTTCTC AGGTGTTAGC GATGCCCGTT CATCATTTCC CAGTGGGCAG CTGCCTGACC
    ACGCTGCGTC TGTGTTCTGA CGGCACATAC TTGTATTGGG TTTGGTCTCC GGTCAGCATC
    AACGAGAAAA CGCAGAAAGG CCACTCTGTC TTCATGGACG TCTTCCAGTT AACGAGTGAG
    TCCGGTGTGT GTGTGGCCAG TCCTGTACAA GACCGGGTGA TCCTGTTGCG AAAGGAAGGC
    GAGTCGTCCA AGTGCTTGAA TGAGTTGCTC CTCAGCCGAA TGTCGCGCTT CCGAGCCTCG
    CACTCGTCCA CACTGGCGGC TCTCACCGGC TCCGCCATCA CCAACCCTGT CAAAGAGGAG
    CAATCGGTGG TCAACACAAG CTGTGGTCTG TCCATCAAAA CACTGAGGAA GACACCCATG
    TATGTGTGCG GGACGTCCCT GGTGATGCTG GTGCCGACTC CAGGTGTTGC TGGGAGCTCC
    GCCACGCGCT CGCTGTTCGG GGGATCCAGT GGCCTTTCTT CTCTCAAGAT CTTGGCCTCT
    AGTTTGGTGT TTAACCTGGC GGACGGTCAG TTCAGCAATC GCGTGGATCT GGTCGACGCT
    CCGGGAAGTT CTCTGGGTCG TGGAGCTCAG GTTGCAGGAC TCGGCGCCTG TTATGATGCA
    CTGAACAACT TAATCTGGAC GTGTTCGAGC GACTACATGG ACCAGTGGTG CAACCCTGGA
    AATCAGGCCT TCCATCATGT GTGCCAGCGA CTCGGCGTCA GTCACGCCAT CAGCCAGACC
    AAAGAAGAGA TGATCGACAC ATCCAGCGTG ATCAGCCAGC TGCTCCATCA TGTGGGAGCC
    ATGTGTATCC ATCAGCTGAA CCTGCTGGCC GCCAGCAGTA GTTTACACTT GGGCACCTTC
    ATAGGGAAGC AGCCAATGGA GGCCTGTCAC TTCAGCTCCA TCTGTGACGT GATGGAGAAA
    GCCATGGTCA ACGGAGACAC CCGCATCATC CGCTGCATCC TGGTCGTTTT TCAGGTGGTT
    TTCCAGTTTT TTAATCCAAA CTCAGAGCAG AACCGGGAGT GTGTGAAGCG CTCAGGACTG
    CTGCTGTGGC AGTTACTCAT GGCTCCACCT GATCAGATCC AGCCGGAAAT ACAGAAAGAA
    GTTTGTCTCG CCATCAGCTC AGGTTTAAAC ACCCTGTATC AGAGCGAATC TGAGTTGAAC
    AACCTGCTGA AGTTTGTTTT CACAGAGGGG GAGAAGAACA GTGGTTTGTC TCAGTTACGA
    GACGTGATTT TAAACAATCT GGCTAACCAG TTACAGAAGA ACCGCTTTGG TAGTGAAGAT
    GATGATCACT ACAGGCTGAA GGATGAGCTG CTGCAGTGCA TCTTGAAGAC GGTGGTGCGC
    GAGTCGTGTC TCCTCATCAC CAAATGTCAA ACCGTTTCCA AAGAAGACCT TCAGAAGCTG
    CTCTCTACTG TACCTGTGGT GTCTCCCAGC TTGCGTTATT TAATGGCTGT GCAGAATCAT
    CTGCTCAGCA ACACCATCCT GCTGCACCCG GAGGACATGG AGGACAGCGA CAGCTCCCTG
    CAGGGGGAAA CAATGAAGGA GCTGCAGACG AGCATCTTGT CTCTTGCGTT GAAGATTCTG
    GTTGGATGTG ATGAAGTTCT GGAGACGCTT CAGCAGGTGA CCAGCTCTCT CATCAACAGC
    AGCATCAGCG ACCGAGAGAC CAGGCTCAGA GGTCTTGAAC AGGTGACCAA GGCCACCATG
    TTGGGTCATT TGCTGCCGGT TCTGCTGACG TCACTGATGC ACCCGAACCT CCAAACACTC
    ACGCTAGCAG ATGCGCTCAT GCCACAGCTA GTCCAGCTCG TCCTCTACAC TAGCCAGACC
    GCTTTGCTGC TAAAGACACA TTCTCCATTA TTTGTTGAGG ATTCCCCAAT AAATCAAGGC
    TCCAGCCAGC CCTCATTAAA TGACAGTTTT CAGATTCTGG AGGAGAGAGA GGAGCCTGGC
    TTTCTGACGG GGCTGAAGAT CCCCGCCCCG TGGGCTGCTG GGAAAACGGT GGAAACTGTT
    CACCCGGTGC GGGATAACTA TAAATTTAAA GACACAGTGC ACATTCCTGG AGCTCGCTGT
    CTGTACCTGC GCTTCGACCC GCGCTGCTCC TCACAGTACG ACTACGACAA GCTTGTGATT
    TACGCCGGGC CGAACACAAA CAGTCGTAAA GTAAGCGAGT ATGGAGGAAA CACCATGGGT
    TATGGCAGCC GCAGTGTATT GGGAACCGGA TGGCCAAAAG ATCTGGTCAA GGTGGAAGGA
    GATACGGTGA CGTTCTCTTT TGAGATGAGA AGTGGACGCG AGCACAACAC TCCAGATAAA
    GCCATGTGGG GTTTCTCGTG CACCGTCAGA GCGCAGGAGT CGTCTGAGGA TGTGTCTGGC
    GGTCTGCCGT TTCTGGCTGA TCTGGCTCTG GGTTTATCAG TGTTAGCCTG CGCTATGCTA
    CGAATCCTCT ATAATGGGCC TGAAATCACA CGAGAGGAAG AAGCTTGTCA CGACCTTCTC
    TGCTCCAAAT TACTGCAGAG GTGTCAGTGG CAGGTGGAGG CGAACGGAGC CATCTCTCCT
    GCACTCACGC CATCTCCGTC TCCTCTTCCT CTGACTATCG ATGAGGAGCG CGAGTTCACA
    TATCCATCAG ATATCCTCGC TCCGCCGCCG GGGCCCATCC CGAGCGCCTG CTGTGAGCTG
    CCCCGCATTC ACTTACCGCA GGGTATCATG GGCCGCCTGA GGGAGCTGTC TGGACGTGCT
    CGGCCACAGT TTCGCCCCAG CATCAAAGAG GTGATCCGTC CAGACGTGAT GGAGGAAGTG
    ATTGTGTCGT GTGTGATTAA ACACTTGGGT CTGGTGGACG CGCTGCAGTC GCTGGTCAAC
    TTCCAGTACA GAGAATCTCA CAGAGAAGAA CACAACCTGC TCTGCAAGAT CATGGCTGAG
    ACCTTCAAGA AGATCAACGC CATGGAGAGA CAGTTACAGA GCGTGGCTGA GCTGGAGCAG
    AAGTGGCAGA ATGAAGTAGA TGAAGCTCAG CAGGGAAAAC TGGAGAACAA CACTCCTTTC
    TTCCACGATT ATCACTTCTT TGAGAATAAA ATGAAGGAGC TGGAGCTTCT CTGTTCGCTA
    AAGGAGGTTC CTCTGGACTG CACTGATCCA GAAAACGCTG TGATGGCTTT GAGAGAGAAG
    TTTTTTCATG AGGTGAACTC CACGCTGCAG CAGCAGAGAA GTGTTGTTCC TCTGGCTAAA
    ACCAAAGCGC TTGTGAAGAG TTTGATCAAC CGCTCGGAGC TGCTGCTTCA TGTCACCATC
    GCTCCGCAGT GCAAGAGTCT GTCATCAACA CCCACAAGCA CACCGGCCTG TAAGTCGGTG
    TCAGACGCTA AATCGATGGC TCCCGTGGTG AAGCAGCCGG TGTTCCTGCG CAGCATGTCT
    GCTCCGTCAG ATCTGGAAAT GATCGCCAGC AGTGATGTGG AGTTCACACA CGGAGCGCAA
    AGGAGGCGGC AGCACCCCTC CAGCCACCGG AGCAGCTCAT TTACACTCCT GCAGTCATTG
    GTGATTGAAG ACAGTAAAGA TAAACCCACG TACAGTGTGC TGCTGGGACA ACTGTTCGCC
    TTTATAGGAA CCACTCCTGA CCAAACCGTG TCGAGCAGCA GTTTTCTGTC CGCAGCTCAG
    ACGCGCTGGA GAAGAGGACG AACACGCAAA CAGGCTCTGG TTCACATGCG GGAGCTGCTG
    ACCGCTGCCG TCCGCGTCGG TGGAGTCACT CATCTGGTCG GACCCGTCAC TATGGTCCTG
    CAGGGGGGAC CCAGGGTGGA GGAGCTCACC TGTGGTGGGA TGATGGAGCA GGTGCAGGAG
    GCGTTTGGAG AGACCATGAC GTCAGTGGTG TCTCTGTGTG CCCGATACCC CATCGCCTGT
    GCCAACAGCA TCGGCCTGCT CTGCACCATC CCCTACACCA GGAGTGAGGA GCAGTGTCTG
    GTGCGCAGCG GTCTGGTTCA GCTCATGGAT CGCCTCTGCA GTCTGAGCAG TCAGAGAGAC
    TGCAGCTCCA ACGAGAAGCA AACCAAAAAG CAGAAAGTGG CCACCATGGC CTGGGCTGCG
    TTTCAGGTGC TGGCCAACCG CTGCATTGAG TGGGAGAAAG TCGAAGGAGG CTCCACAGAC
    GCAGTGCATT CAGGGTTGGC TCGACAGGTG TCCAGCCTTC TGACCAATCA TCTGGCTCGA
    GCGACAGACT GCTGTGGAAA CCAGGCGGCA GGAAATGATG CGCTGCAGGA TGTGCTCAGT
    TTACTCAACG ATCTGTCCAG GAGTCACATA GGCAAGACCA TCCTGAGCCA GCCTGCCTGT
    GTGTCCAAGC TGCTGTCTCT GCTTCTGGAC CAGCGACCGT CGCCCAAACT GGTGCTGATC
    ATCCTGCAGC TGTGCCGCGC CGCGCTGCCC CTCATGAGTG TGGAGGATTG TGGAAATGTA
    GCGCTGCCGT CCTGGAGCTA CTCCACAAAC GCACTGGACG CCGAGCAGCA GGACGCCAGC
    GACCCTGCCT TCCGCATCGC ATCTCTGCTG CTCGCCAAAC TGGCCGACTA TGTGGTGCCA
    GGCTGTCAGA CGTTGCTCTC TCCCAGCGCC TCTGAGCCGG ACACCAGTCT GAGCCGAGCC
    TGTCCCAAGA GCAGCTCTAA GACCGACAGA GATGCCAGCG AGGAGGGCGA AGCGGTGGAC
    GGAAAACTCT CCATATTCAT CCATAAGAGA GAAGACCAGT CGTCGCACGA GGTGCTGCAG
    CCTCTGCTCA GCAGCTCAGA GGGTCGACCG TTTCGTCTCG GCACTGGAGC CAACATGGAG
    AAGGTGGTGA AGATGGATCG AGACATGACC AAGAGCGGCT GCTGTGAGGT CATCACTGAA
    GAGGCGTCCG CTGCTCTCAG GAAAGCCAAT AAATGGGCTC AGTCGGGGCT CATTGTGAGT
    GTGGGTCCGC CCATCGAAAG CAGCGCTCAG GAGAACTCAG GAGCGACCTC TACAGGAGAC
    AAGAAGAAAA GCGCTCAGTC CACGGTGTGT CGAGAGAGGA ACGCTGAGCT CGCCCGGTCT
    GACCCGGTGC GTCCGTTCAT CAGTGGTCAC GTAGCCAACA GTATGGCAGC AGAGGTCATC
    GCTCTGCTGC ACAGCCTCCT GACGGCCCCC GAGTCCAACA CTGCTCAGAT CTGGACAAGC
    ACTGCAGAAA AGGTGTTGTC CAGAGCTCTG ATGTTCATCC CTCAGCTGGG GAAGTTTGCT
    GAGAGTATTC TGGAGAGCGG AAACAGCAGC GGCAGGAAGT TGTCCAAGCT GCAGGCCATT
    GGTCGACAGG CGGTGGCGGC TCTCTGTGCG CTCGGCGGAT TCAAGGAGAC CATCAAGATT
    GGATCTGAAG TGCAGGTGGT TGGTAAAGGT GTGTTGGACA GTGTCGGTGT GGTTATATCC
    ATTAATGAGC AGGAGGGCAT CGCCACCGTC AAGTTTCCAT CCTGCGAGTA TCGGCGCTCC
    AGCAAAGCAT CTGATATCCT GACGGTGCCC ATATCACGCC TGTGCACGCC GCGCTCAGAG
    GCTCTGCCGC TGCACAAGCT TTCCATCACA GAGAAGGTTG TTCAGGCCGT TCAGTCCATG
    TTGCTGCCGC AGGAGGGCAG TCTGTCCATA CACACGTCTC TGCCGGCGTC TGGAGACGGC
    TCCAGTCCTG TGATGGCAGT GGTGCGTCTG CTGGCCGAGA TCAGAACCAG AGCGTGTCTG
    GTGATGGCGC AGCTGCTGGA GGACAGCTCT TTCTGTGAGG AGTTTATCCA GCAGTGTCCT
    GCAGCCGTGG AGGTGCTCAA CCTCATCGCT CAAGAGTGCA GTCCTGGAGA GCGTTTGGTG
    ATGGTGGAGG CTCAGTGTGA GCGTCTGCGC ATGCTTTATC GAGACTGTGC TCGTCCTCCA
    CCACCGCCTG TGCAAACTGA CCAACGGCAG CCGAAGGAGA TCACGTGGTG CCCGTCTCGA
    GTCTTCCCTC CGGTTCGGGC CTGTATGTTT TCCTCGCACT TGACGTCGGT GACGTTTCTG
    GCTGATCCGA GTGCAGGCGG AGGCCTTCCC AGAGGAACCT TCATCTACGC CACTTCACCC
    GTGCCCATTC AGGCTCCGTC TTTTTACTGG GAGATCGAGA TCGTTTCGTT CGGAGACTCG
    GAGGACGACA GCGGTCCAAT AGTGTCCTTT GGATTTTCCC CGGAGGCGGA GAAGAGAGAC
    GGAGCCTGGA CCAATCCCGT CGGGACCTGC CTGTTTCACA ATAATGGCCG GGCGGTTCAC
    TACAACGGCT CCAGTTTACT GCAGTGGAAA AGTGTGAGGC TCGACGTGGC TCTGCTTCCT
    GGTGATGTAG CCGGCATCGG CTGGGAGCGT CTGGAAGGCA CTCCTCCTCC ACCCGGTCAG
    CCTCCCAAAG GAAGAGTGTA CTTCACCTAC TGCGGTCAGC GACTCGCTCC CTACCTAGAG
    GATGTGGCTG GAGGCATGTG GCCCGTCGTT CACATCCAGA AGAAGAATAC GAAGATTCAC
    GCCAACTTCG GCTGCCGGTT GTTTGCGTAC GCTGAAGGCC AGGCTCACAG AAACGCTGCT
    GATCTCTGCA TGGATCTGTC AGAGGAGATC AGTGCTAACT TCGAGGCTCT GCCCTTCGCC
    ATGGCCTCCG ACAGCGATAA TGATGCGGGC ACCAGTGTGG CGTCTGACTC CGGCTCCCAC
    GGGCCTCCTT GTCGCATCGC TGCCGTCGTC ACAGCACAAC AGCAATACAA CAGCGATGCT
    TCTTGCCACT ACAAGGTAGA GCTGAGCTAT GAGAATCTGG TGGTGTCGGG ACCGAACGCT
    CATCCGCCAC CCATCGCTGA TGACGAGAGC GATGATGAGG ATGATGAAGA CATTCCCCGG
    GAGGATCATT ACGCCCTGCT AGTGAAAGCG TGGGAAACTA AAGTGTTTCC CACCATTCGC
    CGGCGGTTCA GGAACGAGGC GGAGAGGAAA TCTGGCCTGG ATCAGATCAA GGGAGCGCTG
    CAGCTCGGTA TGGTGGACAT CGCTCGTCAG ACGGTTGAGT TTCTGTATGA AGAGAATGGC
    GGGATCCCTC GAGACCTCTA CCTTCCAACC ATCGAAGACA TTAAGGATGA AGCAAACAAG
    TTCACCATCG ACAAAGTCCG CAAAGGCTTG ACGGTGGGCA TCCGCTGTCC AGAGGGAAAC
    AACACCTGCA GCAGCACAGG AGGAACCGCT CTACCAAAGT TTGCCATCCG CGGCATGTTG
    AAGACCTTCA GCTTACATGG AGTTGTGCTC GATGTGGATT CGGGTAATGA GCTGGTGCAG
    GTGGAGACGT ATCTGCGCAG TGAGGGTGTG TTGGTGCGCT ACTGGTACCC CATAGAGATG
    CTGGAGAGAC CGCCGGCAGG ATCCCGCAGG GCTGCAGCCA ATGGCCTGGT GTCTTTGGAC
    AGCAGCAACA TCCAGATTCA TCGGGAGCTG CTGCGCTGTG AAAGTGCTCT GGCGCGTCTG
    TACTGCCGCA TGGCGCTGCT CAACATCTTT GCGCCCAAGA GTCCTCACAC ATTCACGCGC
    CTCTTCCACA TCCCTGCAGT GCGTGACATC ACACTCGAAC ACCTGCAGCT CCTGTCCAAT
    CAGCTGCTGG CTCCGCCTCT GCCAGACGGC CGGATCAGCT GTAACTCTGT GCTGCTGGCT
    CAGTCGGTCC TACATGAACT GCAGGGTCAG AGCTGCTCTC CCACTGAGCT CTTCTATCAG
    GGAAACGCTC AGAGTGTCCG CGAGTGGCTG AGCGTGGCCA TCAGCAGAGC TCTGCATCAG
    GGGGAGGACA GTCTGCTGGA GCTCACCAAA CAGATCTGCT GCTTCCTGCA GAGTGCGCCT
    GAGCAGTTCA CGCTGGAGGA GATTCCCATC ACCGAGTCAA AGGTCAGCAT GGACGTCAAC
    TTCCCCGGCG CTGCGTTTGT GCTCGTCTCC TGCAAAGAGA GTCAACTCAG CTGCCGCAAA
    GATTCCTCTC TGTACAAAGC TCCATGGGCT CGAGTGATGG TGTATGGGCT CGGTCATAAA
    GTGCGGCGTA ACGGCCAGCT GAACCTGATG GAGGCCGTGT GTTTCCCGCT GGACGCCTCT
    CCGTCCAACA CGGGCCTCAC TCCTCCACCC ACAGCCAACC AGTATCCCAG CATCATCATC
    CCCACCGACA GAGTGCACGT CAAACTGGGT GTGTCTCCCC CTCCTGGAGC AGTGCTGGTG
    TTGCACTCGT TGCCTCTTGA GTTTCCATTG GCGATGGCCT TCGCTGAACA GCTGCTAACC
    TACAGTGTAG GAGAGACCGT TGAGCGCTCT GAGGATGAGC TGGACACGGT GCCCACCTCT
    GTGCTCATGC AGGTCGTGGA GCTGCTAGGT GGCCTTTTGT GGACCACAGA TCTTGCTCCG
    AGCATCAAGG AGCTGACCTT CCACCTGCTT GCAGAGCTCC TCCGCAAGAT CCACGCTTTT
    GAGCGCCGCA AATCCCCTTC GGGTCTCTCT AGCCACATCA CGCTGCTTCT CAACCCCTGC
    CTAACAGTCC TGATGGTTCT TCAAACCGAA CTACGAAAGC TTTACGACCA CGAGACCCAA
    AGCTGGGGGT TGGTTAGCGG AGGATCCTGC ACTTCCGCAA CAGGCGGCAG TGGAGCGCTT
    GCACTTTCTG CAGAGCGGAG CCGCTTCTCT ACATACTTCC ACGCACTGAT GGAGGTTTGC
    CTGGCAGTGG CGGAAGTCAC TTTACCGCTG AGTTCTGGTT CTGGGACGTC AGTAATCGGC
    GTCCCTTCAT CAACAAGCAC AGCCAACTGC AGTGATTCCT CATCCTCTTC TTCGTCCTCT
    CCAGGCCAGA CGCCGCAAAG TCCGAGTTTG CTTTCAAAGC GCAAAAAGGT GAAGCTAAAA
    CGTGAGCGCG CTGCAGTCGG CTCAGCTTCC GCCGTCTCAG GGAAAAGTGG CTCGAGATCA
    TCAGAATGCG ACAATGCTCT ATTAAACATG AAGCCTGAAG ACATGTTGTG GTTTCACCGG
    GGGTTGACCT TGCTTTTGAT TTTACGGCAT CTTACTAATA AGGACCCGCA AGGTCTTGGC
    GTCACCGGCG ACGCTGTGGC AGACGCTTGC CAAGCTTTGG TTGGCTCGAC AGCTCACAGT
    CGCTTGCTGG TGCTCTCAGG AATCCCGACT CACCTGGAGG AGTCTGTGGT GCGCAATGCA
    ATACGAAGAG CATGCAATGC TCATGGCGGG CTCTTCAGGG ATGAGATCTT CATTCCAGTT
    CAGGAAGAAG ACCCTAAAAA AGCCAAAGCG GAAACGACAT GTGTTCCTCA AGATGTAAAG
    AGAGAATGCA CTGCAGGAAG CCCCAACAGC AACAGTGCGG CAATGAGTGT CTCCAGCGCT
    TTACAGGCAG CCTTCAGAGC CTCCGGAGAG CCCTTGTCCA ATCAGGAGAT GGTAAACAGT
    CCTTCCCAGC AACAAGCACC TCTGGATCCA CTCACAGTGT CCAGTCAGGA GAGTCTGGAC
    GTGTCTCTGT GTAGCACAGA AAGTCAAGGG AGTCTCGGCA GCCTCGGGGA GCAGGATAGT
    GCTGCCACGT CAGTCTCAGA TGGGAGCTCC ATGTACACGG TGGCCTCACC TGATGTTCAT
    GCCAGTGTGA CGCGTCCCAT AAAAGGCTAT GCTGTCATTG AGGTAAGGGC ACGAGCTCAG
    GTGGAGAAGA TTCGGGCCAG TCTTTTCAAC AGCAGCGATC TGATTGGCCT CTCCAATCTG
    GAAAGGGAGG AGGAGCTAAT GGAGATGACC AATGAGGAGA TCCTCACCGC CTCCAGTGTC
    AATCAGAGTT TATTTGACAC ACAGGGCAGC TCTGCTCTGG AGGAGTACTT CCTGGATAAA
    TCCATCAGAG GAGACAAGCT GGTTCCTGGA GCTCGTGACG TGCTGACAGA CATCTTTAAG
    AGCTGCATAT ACGCAGAGCA GGCGCTGAGC CCCGTTCCCG TGAAACCTGT CAAAGTCTCA
    GACATTTACC TCAGCAAAGA GCAGATCAAC TCTCAGTCTC CCGGAAACCT GCTACACGTG
    TTTTTCACAC ACGTTCGGCC TCCCAAAAAA GTGCTCGATG ACCAGCTCAC ACAGATTCTG
    CGGAAATACG GCACAGTAAA GCCCAATAAA AACAAGCACA GCAAAGCGGG CAAAGAGCAG
    CACCAAGGCA AAGTGGTGAG CACAAAAAGA CCCATCACCA AACCTCCGGC CAAAGAGAAG
    TCCATGCTGA ACAGCGTGCG AACTGTACTG AGTGAGAAAA AAACCGTCCT CAAGCCAAAA
    TCTCCAGAGA AGACCAAACC CGATGAAAAA GACCCCGAAA AATCTCCTGC CAAAAAACCC
    GAAGTGCCAG AGGAGAAGTT TTTGACGCTG GAGGGTTTCC ACAAGTTTAC TCTGGACAGA
    GCCAAGCAGG ATATCCGCAG TGTTTGGCGG GCGATCCTGG CCTGTGGATA TGACCTGCAC
    TTTGACAGAT GCACATGCAT TGATTCCCGA CATGCTCAGA AAGCCAGCAG AAAATGGAGC
    TTCGAGATGG ACTTTGCGTT GGTGCAGTTT GTGAACCGTC TCTGTCGTCA CCTGGCCATC
    ACTCCTGCCC GCCTGCATCC ACATGAGGTT TACCTGGACG CCAGCAAAGA TGGCGCGGAT
    CCTGAGGTCG CGTGTCTGTT AGGTGTCCCA GTGGAGAGTT TGCGTCTGCG CTTTGCTCTG
    CTGCAGTCCC TCAACAGCAC GCTGGAGAGC TTCTTCCTGC CTTTAGTGGA GCTGAGACTC
    ACACAGACCT ACCAGAACAG CATCGCCACA CTTCTGTGCG AGGCCAAAGG CCTGATATTT
    TACGACACTA AGGTGACGGT GATGAACAGG GTGTTGAACG CTACCGTGCA GCGGACGGCA
    GATCACGCAG CGCCTGAAAT CACTCTCGAC CCGCTGGAGA TTGTTGGAGG AGAGATGCGC
    GCTGCGGAGA ACACTTATTT CTGCCAGGCA GCGCGGCAGC TGTCGTGTGT GCCATCGTCT
    CAGCTGTGTG TGCGCATCGC CAGCGGAGGA GACCCCACAT ACGCCTTCAA CATCCGCTTC
    ACCGGAGAGG AGGTCCACGG CACCAGTGGC TCTTTCAGGC ATTTCCTATG GCAGGTGTGT
    AAGGAGCTCC AGAGTTCAGC TCTCTCTCTT CTTCTGCCCT GCCCGAGCGC CGCCGCTAAC
    CGCAATAAGG GGAAGTACAT CCTGACTCCG TGTCCCATCA CATACGCTGA GGAGCAGCTG
    CTGAACTTCT TCGGGCAGCT TCTGGGCATC GCCATCCGGG CCGATGTGCC GCTGCCTCTG
    GACCTGCTGG GATCCTTCTG GAAGGGTCTG GTCGGAGAGG CTCTCGATCC CGAAGCTGAC
    CTGCGGGAAG CAGATCTGCT CACGTACAGC TACATCAAGA AGTTTGAGAG TTTGTGTGAT
    GAAGCGGAGC TGGAAGCGCT GTGTGCTGAA GTGTCGTCTC AGAGTCCCCT GGGGGAGAGT
    CCAGACTCCC CCAGCCGGCC CTGCTGCACC TTCACATACA TCACTATGAC TGGAGAGGAA
    GTGGAGCTGT GCCAGGGAGG ACGTGACATC GCTGTCAGCT GGGAGAATAA GGATGTGTAT
    GTGCGAGCGG TGCGTGAGCT GCGTCTGCGG GAGCTGCAGA ATGTGGAGTG TATGTCTGCG
    GTGCGCGCCG GTCTGGGCTC CATCATCCCA CTGCAGCTGC TGACTTTACT GAGTCCCGCA
    GAGGTGGAGC TGCGCACCTG CGGTCTGCCG GACATCAACC TGGAGTTCCT GAAGGCGCAC
    ACCATGTATC AGGTGGGTCT GATGGAGACG GATCAGCATA TCGAGTTCTT CTGGGCGGCG
    CTGGAGATGT TCACGCAGGA GGAGCTCAGC AAGTTTATTA AGTTCGCCTG CAACCAGGAG
    AGGATTCCCT TCACCTGCCC GTGCAAAGAT GGGGGTCCGG ACACCGCACA TGTGCCCCCC
    TATCCCATGA AGATCGCCCC GCCGGACGGC ACCGTGGGCT CTCCAGATCA GCGCTATATC
    CGCGTGGAGA CGTGTATGTT CATGGTCAAG CTGCCGCAGT ATTCCTCCCT GGATGTGATG
    CTGGAGAAAC TGCGATACGC TATTCACTAT CGAGAAGATC CTCTGAGCGG ATAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.