LOC101884666 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC101884666
Entrez Gene ID	101884666
Full Name	protein unc-13 homolog C-like
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009293377.3	XP_009291652.2	protein unc-13 homolog C-like isoform X2
XM_005170800.3	XP_005170857.2	protein unc-13 homolog C-like isoform X1
XM_009293377.2	XP_009291652.1	protein unc-13 homolog C-like isoform X2
XM_005170800.4	XP_005170857.3	protein unc-13 homolog C-like isoform X1

Pathways from BioSystems
Gene Ontology
Bibliography

Related articles in PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
Pasquier J, Cabau C, Nguyen T, Jouanno E, Severac D, Braasch I, Journot L, Pontarotti P, Klopp C, Postlethwait JH, Guiguen Y, Bobe J
BMC genomics17368(2016 05)

GeneRIFs: Gene References Into Functions What's a GeneRIF?

The following LOC101884666 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC101884666 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa149910	XM_005170800.4 Latest version!	Danio rerio protein unc-13 homolog C-like (LOC101884666), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1371.30 $1959.00
ODa149911	XM_009293377.3 Latest version!	Danio rerio protein unc-13 homolog C-like (LOC101884666), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$1161.30 $1659.00
ODa51416	XM_005170800.3 Latest version!	Danio rerio protein unc-13 homolog C-like (LOC101884666), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00
ODa51417	XM_009293377.2 Latest version!	Danio rerio protein unc-13 homolog C-like (LOC101884666), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	$797.30 $1139.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC101884666 ( XM_005170800.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170857.3 Danio rerio protein unc-13 homolog C-like (LOC101884666), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa149910
Clone ID Related Accession (Same CDS sequence)	XM_005170800.4
Accession Version	XM_005170800.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 7020bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein unc-13 homolog C-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) and transcript sequence (GFIL01022384.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005170800.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 5039 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGTTTCTG CCCATTTCAA AAAGCTATCG TCTTACATTG TAAAGATTTG CAAAGGAATG 
TTTACAAAGA AACTGGCAAC TACCACCAAG AAGAAAGAGA GCAGCGAAAA CAAAAAAGAA 
CCCAAATCGA CGTCCCCTGA ATTGCACGCT CGCAACCCGA CGTTCTCCAC CACACTAAAA 
AGCACAGTTA AAAAAATCTC CAAATGCTCA TCCACACGCA ATATATCTCT TGAAGAGGAT 
GACGGCAAAA CCGACTGTTC TTCTCTCTCG CCAACATTCA GTTACCGCGT AGCAATTGCC 
AACGGACTGC CCAAAAATGC CCTGTTTCTG AACAATGAAT TTCATGAGGT CTTGTCAATT 
GATAGCGATT ACTCTGACTC TCTGAACGAG GCAAAACTTG TCCATAGATT CGATGAACAA 
AAAGCTTACA CGATGCCAGT AAGAAGGAAT CGGAAGAGTT TGATTAGTTT GGCGCCCTCC 
GATGGCAGCT CGGAGGGGGA ACGTGGAGAG CGCAGCAGTC TCCACACCCT CCGATTGGGC 
GCCTTGAAAA AGCTAAGGAA ATGGAAGAAG AGCCAAGAAT GCGTGTCCTC AGACTCGGAG 
GCCAGCAATT GGAGAAAAAC TCTTGGCATT CGAAGCAAAT CTTTGGACAG GGCCGGCCGA 
CACCAGAAAA CCACTACCCT GGAGCCAGGA TCTAGCTCCA CGGGATGCAT CAGTCAGACT 
CAGGATGTGA TGGAGATGAT TTTCAAGGAG CTTCAGGGAA TCAGTCAGAT CGAGTCCGAG 
CTCTCAGAAC TGCGAGGGCA TGTAAACGCC CTCAAAAGCT CCATCGATGA GATCTCCAGC 
AGTGTGGAGG TGGTGCAGAG TGAAATCGAG CAGCTCCGTT CAGGTTTCGT GCAGTCCAGA 
CGCGAGACTC GAGACATTCA TGATTACATC CGACAGATCA GCCAACAGAG CAACAAAGCT 
ACTCTGAGAT TTCTAAATGT GCCTGAGGAG AAGTACGAAA AAACCGAAGA ACTTATCTAT 
CAGATCCTAA AAGAGAAAAT GGGTTTCATT GATGCACGTA AGGCATTTAA AATTGAACTA 
GCCCATCGGC TGGGGCAACA AAGAGAATGT TACAATGCTA AACCACGACC AATTATAGTT 
ATATTTGCAA GCCCGCAAGA CAGAGATTTA GTTTTGAAAA AATGTTATAA GCTTAAAGGG 
ACTGGAATAT CCATCTCCAC TGACAGTCTA GCACACGACT CGAAAGAAAA GAGAGAAAAG 
CCGATAGCTT CATCTCAGAC ATACGAAAGC ATGGACATCA AGGTCTCGGC TAAAGACAAG 
GCTGAAAGTG ATGACTGGGA CTCCATGGAA AGCGACAAAG AGCTTGATGA GTTAAACAAG 
AACAAATATG CAATTGTGTC CAAACCACTT CCAAAAAGTA AATCGGACAA GAAAAAATCC 
CACGATCACA GAAGAATGGG TGATGACTCT CCATATTCTG TACACTATGC GGATAACACA 
GCCTACGATG ACCCCGACAA GCAAAGCAAG TCCTACTACT CTGACATTAC ACCTGGATGG 
CTGTCTCAGA GCGACTACTC CACTCCCAAA CTTAGCCGAT CTGAATCAGA CTGCTCTAAA 
CTTTGCCAGT CTTACTCTGA GGACTTCTCA GAGAGTCAGT ATTTCACCAG AGTCAATGGC 
TGCTCTTTGC TTTCGTCCTC AGATCAGGAG CTTTGGCAGC GTAAGCAAGA AGACATGGCG 
TCCTCCTGGT ACGCCAGTCC CCCCAGTCAA ACTCTGAGCC AAGATCGACC TTATGTGGAA 
CATAATGAAG TTGAAACCAC AGAGACTATT GACAGTGGTG TAAGTAATGG TCTTGTTTGC 
ATATCAGGAG ACAGGAGCCA TTACAGCGGC TCCCAGCTAT CGCTGCAGGG TGACCTCTCG 
CCATGGAAAG ACTGGCATCA CCTTGAACAA GGTGCTGATT CAGGTTTGGA TGCCTCAACT 
GAACAGAACA TAATCTCTGA AATCAGTAGC CCATTTGACC CAGCAGCTAA CCCTGGTTTC 
CCTGAAAAAA TTATTAAGTG TCTTGAGGTT GATTTGCAAT TTGAAGGTGA GACGTTCTTG 
ACACTAGATA GCACTCCTGA GACAGGAGAC CAAGATATAG ACATGGAGCC TGAGTTTTTA 
GAACCTGAGT TAGAACCTGA AGTTGAGGCT GTGATTGAAC CAATGATTAA ACAAGAACGA 
GAACCAATGC CACAGCCAGA ACCTGAACTA GAACCTGAAC TAGAACCTGA ACCAGAACCT 
GAACCAGAAC CTGAACAAGA ACCAGAACCA GAACCAGAAC CAGTGATTGA ACCCATTCCA 
AAACCAGTAC CAGTATCAGT ATCTAAACCT GTGGTTGAAC AGCAAACCGA AAAGAAGCCT 
AAAACCAAGA AGATCCAAGC TAAACCTGAA TCAGCTCCAC AAACTTTGCA GCGACAAGAT 
GTGAACCACC ACCTCCAGCA GAACCAGAAA TCCTCAGCTA TGTACCGGAG CCAGAGTGAG 
ATTAGAAATG AGAAGGTTGA GGATGTCCCC AAATCCTGGA GTAGCAGGCT CAGCATCGAC 
CTCAGCGAGA AAACCTTCGG CTTTGGGGGA TTTGGATCCA CCCTGCAAAG AGCAAAGTCA 
GCTTTGGATT TTGTGTGGAA TAAAGGTTCC CAGAGTACTA GCGCACCGGT TGAAGAAACC 
AGCAACACCT CGTTCATGGG CCGGTTCAGA ACCACATCCA CAGCCAATAG CTCCAGTACA 
ACCATTGATT CTGACGTCTA CACAGAACCT TTCTATTACA AGGCAGAAGA AGAGGAACAA 
CAACAGCAAC AACCTGCTGA GCAACCTGTG GACAATGAAA CGCATTATGT TGAGGTGATG 
GAACAAGTGC TAGCCAATCT GGAAAACAGA ACAAACGCAA ACGAACCCGA GGAACAGTAC 
CAAGAAGAGG AATACAATGT CTCACAGGAG TATGATCTCT CTCAAGACGC CAACCTTGTA 
CTGGAATATG ATTGCAGCCT CGATGAACAG TATGATGAGG AATACAGTGA GGACTACGAT 
GACAATCCTA TGACTGAAGA CACAGCAGAA TATGAGGTTA GTTTAGTGGA TTATGTTGAA 
GAAACCGAGG AACCGGAAGC AGAGACAGAG GAACAGGAGG AGGTCAAAGA GGAGGTGCGG 
GAGGTTCAGG AAACATCTGA AATTAAAGGA GAAGATGTGG TAAAAGAGAC CGTAGAGCAA 
GAGGATGAGA ATAAAAATGT TACAGAGGTT CCTGTGCAAG AAGCACCACC CAAAAAAAGG 
ATACGTCCCA CATTCAAAGA GGCAGCACTG AGAGCTTACA GGAAGCAGAT GGCGGAGCTG 
GAGCAGCAGA TCCTGGCAGG AGACAGCACG GCTCTGGACA CGGAGGGTCG TGCCAATATT 
CTGGACCCAG CCAAACTGGG GCTGACCGGC GGAGGGGTTG GCAGTATACT TTACGGCATT 
GACAGCATGC CAGATTTACG TCGCAAGAGG ACCATGCCTA TTGTCCGAGA CCTGGCTTTG 
ACATTGGCTG CTCGAAAGGC AGGCATTGCG TTTGGGCTGG TGAACAGATC GACTCTGAAC 
AACGAGGAGC TGAAACTGCA CGTGCTCAGA AAGACCCTGC AGGCGCTGAT CTATCCCATC 
TCCTCCACCA CGCCACACAA TTTCGAGGTG TGGACGGCCA CGGCGCCTAC ATACTGCCAC 
GAGTGTGAGG GTCTGCTTTG GGGAATCGCT CGCCAGGGCA TGAGATGTAG CGAATGCGGA 
GTCAAATGCC ATGAGAAGTG CCAAGACCTC CTGAACGCAG ACTGTCTGCA ACGTGCAGTA 
GAGAAGAGCT CGAAACACGG AGCCGAGGAC AAAACCCAGA ACATCATCAT GGCCATGAAG 
GAGCGCATGA AGATCAGGGA GAAGAACCGG CCCGAAGTGT TCGAGGTCAT TCAGGAGATG 
TTCCAGCTCT CGAAAGAGGA CTTTATCACT CATCTGAAGA CCGCCAAACA GGCTGTGCTG 
GAGGGAACGT CCAAGTGGTC CGCCAAAATC ACCATCACAG TTTTATGTGC TCAAGGTTTG 
CAAGCCAAAG ACAAGACGGG CTCCAGCGAC CCCTACGTCA CGGTACAAGT GGGAAAGACC 
AAACGCAGGA CCAAGACCGT GTTCGGCAAT CTCAACCCTG TCTGGGACGA GAAGTTCTTC 
TTTGAGTGTC ATAACGCCAC AGACCGTATA AAAGTGCGAG TGTGGGATGA AGACGATGAC 
ATAAAGTCGA GAGTGAAGCA GCACTTTAAG AAAGAGTCGG ATGACTTTCT GGGTCAAACC 
ATCATCGAGG TGCGGATGCT GAGCGGAGAG ATGGACGTCT GGTACAACCT TGAGAAGCGG 
ACGGATAAAT CCATGGTCTC GGGAGCAATC CGGCTGAAGA TCAGCGTGGA GATGAAGGGA 
GAAGAGAAGG TGGCTCCACC TCACGGACAG TACACATGTT TACATGAGAA TCTCTTCCAC 
TACCTGACGG AGGTGAAGAA CAATGGAGTG GTGAAGATCC CGCAGGTGAA AGGAGATGAC 
GCCTGGAAGG TTTATTTTGA CGATGTTTCG CAGGAGATTG TGGATGAGTT CGCCATGCGC 
TTCGGTGTCG AGTCCATCTA TCAGGCCATG ACACACTTCT CATGCCTCTC GTCCAAGTAC 
ATGTGTCCTG GCGTTCCCGC GGTGATGAGT AACCTGCTTG CCAACATCAA CGCTTACTTC 
GCACACACAA CAACTGCAAC CACAAACATC TCGGCCTCCG ACCGATTCGC AGCATCCAAC 
TTTGGGAGAG AGAAGTTCGT CAAGCTCTTG GATCAGCTTC ACAACTCTCT GAGGATCGAC 
CTCTCCAAGT ACCGGGATAA CTTCCCTGCT GGAAATCCAG AGAGACTTCA TGACTTAAAA 
TCAACCGTTG ACCTGCTGAC CAGCATCACT TTCTTCAGGA TGAAGGTTCA GGAGCTTCAG 
AGTCCCCCCA GAGCCAGTAT GGTGGTTAAA GACTGCGTGA AGGCCTGTCT GGACTCCACA 
TACAAATACA TCTTTGACAA CTGCCATGAG CTCTACAACC AGCTGCTGGA CCAGGCTAAG 
AAGCAGGATC TGCCACGCGA GGAACAGGGT CCGTCCATCA AGAACCTGGA CTTCTGGCCC 
AAACTGATCA CGCTGATGGT GTCTGTTATT GATGAGGACA GAACGGCATA CACACCCATC 
ATTAACCAGT TCCCTCAAGA GTTGAATATG GGTAAAATCA GCGCTGAAAT CATGTGGAGT 
CTCTTCGCCT TGGATATGAA GTACGCGATG GAGGAACATG ACAAGCATCG TCTCTGCAAG 
AGCACCGAGT ACATGAATCT GCACTTCAAA GTCAAGTGGT TCTACAACGA GTACGTGCGC 
GATCTGCCGG CGTTTAAGGG CGTCCCTCCA GAGTATTCGC TATGGTTCGA GCCGTTCGTG 
ATCCAGTGGT TGGATGAAAA TGAAGACGTA GCGATGGATT TCCTCAACGG AGCTCTGGAG 
AGAGATAAAA AAGACGGGTT CCAGCAGACA TCTGAACACG CGCTCTTCTC CTGCTCAGTG 
GTGGACGTTT TTACGCAACT CAACCAAAGC TTTGAGATCA TCAAGAAACT GGAGTGTCCA 
AACCCTCACG CCCTCGCTCA CTTCATGCGC AGATTCGCAA AGACCATAAA TAAAGTGCTC 
ATCCAGTATG CAGCCATCAT CTCCAAAGAC TTCAGCAATC ATCTCAGCAA AGAGAAAGTG 
CCCTGTATCC TCCTCAACAA CATCCAGCAG CTCAGAGTCC AGCTGGAGAA GATGTTCGAG 
TCCATGGGAG GGAAACAGCT TGACGCAGAG GCCAGCGAGC TGCTGAAGGA GCTGCAGAAT 
AAACTCAACA CCGTTCTGGA CGAGCTCAGC GCCGTCTTCG GCTCCAGCTT TAAGCCGGTG 
ATCGAGGACT GTGTGAAGCA GATGAACCAG GAGTTGGTGC AGATGAAGGG AAACGCAGGA 
AACAAGAGCA ACGCAGCCAT GGATGCAGAG ACTGTGCTGA GACCCCTGAT GGACCTGCTG 
GACAAGAATC TCATGCTCTT CGCTAAGATC TGTGAGAAGA CGGTGCTCAA ACGGGTCCTG 
AAGGAGCTCT GGAAGATTGT GCTAAACACT ATCGAGCGGC AGATCGTCCT CCCACCGCTT 
TCAGACCAGA CGCAAGGAGC TCAGATGATC TTCAGTGCTG CGAAAGACCT CGGTCAGCTC 
TCCAAACTCA AGGATCATGT GATCCGGGAA GAAGCGCGGA GTCTCAGTCC TCGTCAGTGT 
GCCGCTATGG ATTTAGTGCT TCCTACAATC AAGCAATATT TCCATGCGGG TGGAAACGGT 
CTGAAGAAAA CCTTCCTGGA AAAGAGCCCT GATCTCAAGT CCTTGAAATA CGCCCTCAGC 
CTTTACACTC AACCCACAGA TGCCTTGATC AAGAAATATA TATGCACCCA AACCTCTCAA 
GGTCAGTCCA CAACTGGATC CTTCGGTGAA GTGGCCGTTC AGGTGGACCT GATCTCACAC 
CCTGGAACTG GAGAGCACAA AGTCAGCGTT AAAGTTGTGG GTGTAAATAA CATCAACTGG 
CAGACCAACG CCATGTTTCG GCCCTTCGTG GAGATCAACG CCATCGGTCC ACATCTGGCA 
GACAAGAAAC GCAAGTTTAG CACCAAAACC AAGAATAACA ACTGGTCTCC GAAATACAAC 
GAAACATTCC AGTATGTGCT GAGTAATGAG CACGGCCCCG AGGCCTACGA GCTCCACATC 
TCCGTGAAGG ACTACTGCTT CGCCCGAGAG GACCGAATCA TCGGCATGAC AGTCCTGCAG 
CTCCGAGAAC TGGCCGAAAA GGGCAGTCTG GGCGCCTGCT ATCCGCTCAT CAGGAGCATC 
AGCATGGACG AGACCGGCCT GACCATCATG AGAATACTCT CGCAAAGGAC CAACGACGAA 
GTGGCCAAAG AGTTCGTACG TCTCAAAACA GACACGCGGT CGGCCGAGGA AGTGTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170857.3
CDS	699..7718
Translation	MVSAHFKKLSSYIVKICKGMFTKKLATTTKKKESSENKKEPKSTSPELHARNPTFSTTLKSTVKKISKCSSTRNISLEEDDGKTDCSSLSPTFSYRVAIANGLPKNALFLNNEFHEVLSIDSDYSDSLNEAKLVHRFDEQKAYTMPVRRNRKSLISLAPSDGSSEGERGERSSLHTLRLGALKKLRKWKKSQECVSSDSEASNWRKTLGIRSKSLDRAGRHQKTTTLEPGSSSTGCISQTQDVMEMIFKELQGISQIESELSELRGHVNALKSSIDEISSSVEVVQSEIEQLRSGFVQSRRETRDIHDYIRQISQQSNKATLRFLNVPEEKYEKTEELIYQILKEKMGFIDARKAFKIELAHRLGQQRECYNAKPRPIIVIFASPQDRDLVLKKCYKLKGTGISISTDSLAHDSKEKREKPIASSQTYESMDIKVSAKDKAESDDWDSMESDKELDELNKNKYAIVSKPLPKSKSDKKKSHDHRRMGDDSPYSVHYADNTAYDDPDKQSKSYYSDITPGWLSQSDYSTPKLSRSESDCSKLCQSYSEDFSESQYFTRVNGCSLLSSSDQELWQRKQEDMASSWYASPPSQTLSQDRPYVEHNEVETTETIDSGVSNGLVCISGDRSHYSGSQLSLQGDLSPWKDWHHLEQGADSGLDASTEQNIISEISSPFDPAANPGFPEKIIKCLEVDLQFEGETFLTLDSTPETGDQDIDMEPEFLEPELEPEVEAVIEPMIKQEREPMPQPEPELEPELEPEPEPEPEPEQEPEPEPEPVIEPIPKPVPVSVSKPVVEQQTEKKPKTKKIQAKPESAPQTLQRQDVNHHLQQNQKSSAMYRSQSEIRNEKVEDVPKSWSSRLSIDLSEKTFGFGGFGSTLQRAKSALDFVWNKGSQSTSAPVEETSNTSFMGRFRTTSTANSSSTTIDSDVYTEPFYYKAEEEEQQQQQPAEQPVDNETHYVEVMEQVLANLENRTNANEPEEQYQEEEYNVSQEYDLSQDANLVLEYDCSLDEQYDEEYSEDYDDNPMTEDTAEYEVSLVDYVEETEEPEAETEEQEEVKEEVREVQETSEIKGEDVVKETVEQEDENKNVTEVPVQEAPPKKRIRPTFKEAALRAYRKQMAELEQQILAGDSTALDTEGRANILDPAKLGLTGGGVGSILYGIDSMPDLRRKRTMPIVRDLALTLAARKAGIAFGLVNRSTLNNEELKLHVLRKTLQALIYPISSTTPHNFEVWTATAPTYCHECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAVEKSSKHGAEDKTQNIIMAMKERMKIREKNRPEVFEVIQEMFQLSKEDFITHLKTAKQAVLEGTSKWSAKITITVLCAQGLQAKDKTGSSDPYVTVQVGKTKRRTKTVFGNLNPVWDEKFFFECHNATDRIKVRVWDEDDDIKSRVKQHFKKESDDFLGQTIIEVRMLSGEMDVWYNLEKRTDKSMVSGAIRLKISVEMKGEEKVAPPHGQYTCLHENLFHYLTEVKNNGVVKIPQVKGDDAWKVYFDDVSQEIVDEFAMRFGVESIYQAMTHFSCLSSKYMCPGVPAVMSNLLANINAYFAHTTTATTNISASDRFAASNFGREKFVKLLDQLHNSLRIDLSKYRDNFPAGNPERLHDLKSTVDLLTSITFFRMKVQELQSPPRASMVVKDCVKACLDSTYKYIFDNCHELYNQLLDQAKKQDLPREEQGPSIKNLDFWPKLITLMVSVIDEDRTAYTPIINQFPQELNMGKISAEIMWSLFALDMKYAMEEHDKHRLCKSTEYMNLHFKVKWFYNEYVRDLPAFKGVPPEYSLWFEPFVIQWLDENEDVAMDFLNGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIKKLECPNPHALAHFMRRFAKTINKVLIQYAAIISKDFSNHLSKEKVPCILLNNIQQLRVQLEKMFESMGGKQLDAEASELLKELQNKLNTVLDELSAVFGSSFKPVIEDCVKQMNQELVQMKGNAGNKSNAAMDAETVLRPLMDLLDKNLMLFAKICEKTVLKRVLKELWKIVLNTIERQIVLPPLSDQTQGAQMIFSAAKDLGQLSKLKDHVIREEARSLSPRQCAAMDLVLPTIKQYFHAGGNGLKKTFLEKSPDLKSLKYALSLYTQPTDALIKKYICTQTSQGQSTTGSFGEVAVQVDLISHPGTGEHKVSVKVVGVNNINWQTNAMFRPFVEINAIGPHLADKKRKFSTKTKNNNWSPKYNETFQYVLSNEHGPEAYELHISVKDYCFAREDRIIGMTVLQLRELAEKGSLGACYPLIRSISMDETGLTIMRILSQRTNDEVAKEFVRLKTDTRSAEEVS

Target ORF information:

RefSeq Version	XM_005170800.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein unc-13 homolog C-like (LOC101884666), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170800.4

ORF Insert Sequence:

ATGGTTTCTG CCCATTTCAA AAAGCTATCG TCTTACATTG TAAAGATTTG CAAAGGAATG 
TTTACAAAGA AACTGGCAAC TACCACCAAG AAGAAAGAGA GCAGCGAAAA CAAAAAAGAA 
CCCAAATCGA CGTCCCCTGA ATTGCACGCT CGCAACCCGA CGTTCTCCAC CACACTAAAA 
AGCACAGTTA AAAAAATCTC CAAATGCTCA TCCACACGCA ATATATCTCT TGAAGAGGAT 
GACGGCAAAA CCGACTGTTC TTCTCTCTCG CCAACATTCA GTTACCGCGT AGCAATTGCC 
AACGGACTGC CCAAAAATGC CCTGTTTCTG AACAATGAAT TTCATGAGGT CTTGTCAATT 
GATAGCGATT ACTCTGACTC TCTGAACGAG GCAAAACTTG TCCATAGATT CGATGAACAA 
AAAGCTTACA CGATGCCAGT AAGAAGGAAT CGGAAGAGTT TGATTAGTTT GGCGCCCTCC 
GATGGCAGCT CGGAGGGGGA ACGTGGAGAG CGCAGCAGTC TCCACACCCT CCGATTGGGC 
GCCTTGAAAA AGCTAAGGAA ATGGAAGAAG AGCCAAGAAT GCGTGTCCTC AGACTCGGAG 
GCCAGCAATT GGAGAAAAAC TCTTGGCATT CGAAGCAAAT CTTTGGACAG GGCCGGCCGA 
CACCAGAAAA CCACTACCCT GGAGCCAGGA TCTAGCTCCA CGGGATGCAT CAGTCAGACT 
CAGGATGTGA TGGAGATGAT TTTCAAGGAG CTTCAGGGAA TCAGTCAGAT CGAGTCCGAG 
CTCTCAGAAC TGCGAGGGCA TGTAAACGCC CTCAAAAGCT CCATCGATGA GATCTCCAGC 
AGTGTGGAGG TGGTGCAGAG TGAAATCGAG CAGCTCCGTT CAGGTTTCGT GCAGTCCAGA 
CGCGAGACTC GAGACATTCA TGATTACATC CGACAGATCA GCCAACAGAG CAACAAAGCT 
ACTCTGAGAT TTCTAAATGT GCCTGAGGAG AAGTACGAAA AAACCGAAGA ACTTATCTAT 
CAGATCCTAA AAGAGAAAAT GGGTTTCATT GATGCACGTA AGGCATTTAA AATTGAACTA 
GCCCATCGGC TGGGGCAACA AAGAGAATGT TACAATGCTA AACCACGACC AATTATAGTT 
ATATTTGCAA GCCCGCAAGA CAGAGATTTA GTTTTGAAAA AATGTTATAA GCTTAAAGGG 
ACTGGAATAT CCATCTCCAC TGACAGTCTA GCACACGACT CGAAAGAAAA GAGAGAAAAG 
CCGATAGCTT CATCTCAGAC ATACGAAAGC ATGGACATCA AGGTCTCGGC TAAAGACAAG 
GCTGAAAGTG ATGACTGGGA CTCCATGGAA AGCGACAAAG AGCTTGATGA GTTAAACAAG 
AACAAATATG CAATTGTGTC CAAACCACTT CCAAAAAGTA AATCGGACAA GAAAAAATCC 
CACGATCACA GAAGAATGGG TGATGACTCT CCATATTCTG TACACTATGC GGATAACACA 
GCCTACGATG ACCCCGACAA GCAAAGCAAG TCCTACTACT CTGACATTAC ACCTGGATGG 
CTGTCTCAGA GCGACTACTC CACTCCCAAA CTTAGCCGAT CTGAATCAGA CTGCTCTAAA 
CTTTGCCAGT CTTACTCTGA GGACTTCTCA GAGAGTCAGT ATTTCACCAG AGTCAATGGC 
TGCTCTTTGC TTTCGTCCTC AGATCAGGAG CTTTGGCAGC GTAAGCAAGA AGACATGGCG 
TCCTCCTGGT ACGCCAGTCC CCCCAGTCAA ACTCTGAGCC AAGATCGACC TTATGTGGAA 
CATAATGAAG TTGAAACCAC AGAGACTATT GACAGTGGTG TAAGTAATGG TCTTGTTTGC 
ATATCAGGAG ACAGGAGCCA TTACAGCGGC TCCCAGCTAT CGCTGCAGGG TGACCTCTCG 
CCATGGAAAG ACTGGCATCA CCTTGAACAA GGTGCTGATT CAGGTTTGGA TGCCTCAACT 
GAACAGAACA TAATCTCTGA AATCAGTAGC CCATTTGACC CAGCAGCTAA CCCTGGTTTC 
CCTGAAAAAA TTATTAAGTG TCTTGAGGTT GATTTGCAAT TTGAAGGTGA GACGTTCTTG 
ACACTAGATA GCACTCCTGA GACAGGAGAC CAAGATATAG ACATGGAGCC TGAGTTTTTA 
GAACCTGAGT TAGAACCTGA AGTTGAGGCT GTGATTGAAC CAATGATTAA ACAAGAACGA 
GAACCAATGC CACAGCCAGA ACCTGAACTA GAACCTGAAC TAGAACCTGA ACCAGAACCT 
GAACCAGAAC CTGAACAAGA ACCAGAACCA GAACCAGAAC CAGTGATTGA ACCCATTCCA 
AAACCAGTAC CAGTATCAGT ATCTAAACCT GTGGTTGAAC AGCAAACCGA AAAGAAGCCT 
AAAACCAAGA AGATCCAAGC TAAACCTGAA TCAGCTCCAC AAACTTTGCA GCGACAAGAT 
GTGAACCACC ACCTCCAGCA GAACCAGAAA TCCTCAGCTA TGTACCGGAG CCAGAGTGAG 
ATTAGAAATG AGAAGGTTGA GGATGTCCCC AAATCCTGGA GTAGCAGGCT CAGCATCGAC 
CTCAGCGAGA AAACCTTCGG CTTTGGGGGA TTTGGATCCA CCCTGCAAAG AGCAAAGTCA 
GCTTTGGATT TTGTGTGGAA TAAAGGTTCC CAGAGTACTA GCGCACCGGT TGAAGAAACC 
AGCAACACCT CGTTCATGGG CCGGTTCAGA ACCACATCCA CAGCCAATAG CTCCAGTACA 
ACCATTGATT CTGACGTCTA CACAGAACCT TTCTATTACA AGGCAGAAGA AGAGGAACAA 
CAACAGCAAC AACCTGCTGA GCAACCTGTG GACAATGAAA CGCATTATGT TGAGGTGATG 
GAACAAGTGC TAGCCAATCT GGAAAACAGA ACAAACGCAA ACGAACCCGA GGAACAGTAC 
CAAGAAGAGG AATACAATGT CTCACAGGAG TATGATCTCT CTCAAGACGC CAACCTTGTA 
CTGGAATATG ATTGCAGCCT CGATGAACAG TATGATGAGG AATACAGTGA GGACTACGAT 
GACAATCCTA TGACTGAAGA CACAGCAGAA TATGAGGTTA GTTTAGTGGA TTATGTTGAA 
GAAACCGAGG AACCGGAAGC AGAGACAGAG GAACAGGAGG AGGTCAAAGA GGAGGTGCGG 
GAGGTTCAGG AAACATCTGA AATTAAAGGA GAAGATGTGG TAAAAGAGAC CGTAGAGCAA 
GAGGATGAGA ATAAAAATGT TACAGAGGTT CCTGTGCAAG AAGCACCACC CAAAAAAAGG 
ATACGTCCCA CATTCAAAGA GGCAGCACTG AGAGCTTACA GGAAGCAGAT GGCGGAGCTG 
GAGCAGCAGA TCCTGGCAGG AGACAGCACG GCTCTGGACA CGGAGGGTCG TGCCAATATT 
CTGGACCCAG CCAAACTGGG GCTGACCGGC GGAGGGGTTG GCAGTATACT TTACGGCATT 
GACAGCATGC CAGATTTACG TCGCAAGAGG ACCATGCCTA TTGTCCGAGA CCTGGCTTTG 
ACATTGGCTG CTCGAAAGGC AGGCATTGCG TTTGGGCTGG TGAACAGATC GACTCTGAAC 
AACGAGGAGC TGAAACTGCA CGTGCTCAGA AAGACCCTGC AGGCGCTGAT CTATCCCATC 
TCCTCCACCA CGCCACACAA TTTCGAGGTG TGGACGGCCA CGGCGCCTAC ATACTGCCAC 
GAGTGTGAGG GTCTGCTTTG GGGAATCGCT CGCCAGGGCA TGAGATGTAG CGAATGCGGA 
GTCAAATGCC ATGAGAAGTG CCAAGACCTC CTGAACGCAG ACTGTCTGCA ACGTGCAGTA 
GAGAAGAGCT CGAAACACGG AGCCGAGGAC AAAACCCAGA ACATCATCAT GGCCATGAAG 
GAGCGCATGA AGATCAGGGA GAAGAACCGG CCCGAAGTGT TCGAGGTCAT TCAGGAGATG 
TTCCAGCTCT CGAAAGAGGA CTTTATCACT CATCTGAAGA CCGCCAAACA GGCTGTGCTG 
GAGGGAACGT CCAAGTGGTC CGCCAAAATC ACCATCACAG TTTTATGTGC TCAAGGTTTG 
CAAGCCAAAG ACAAGACGGG CTCCAGCGAC CCCTACGTCA CGGTACAAGT GGGAAAGACC 
AAACGCAGGA CCAAGACCGT GTTCGGCAAT CTCAACCCTG TCTGGGACGA GAAGTTCTTC 
TTTGAGTGTC ATAACGCCAC AGACCGTATA AAAGTGCGAG TGTGGGATGA AGACGATGAC 
ATAAAGTCGA GAGTGAAGCA GCACTTTAAG AAAGAGTCGG ATGACTTTCT GGGTCAAACC 
ATCATCGAGG TGCGGATGCT GAGCGGAGAG ATGGACGTCT GGTACAACCT TGAGAAGCGG 
ACGGATAAAT CCATGGTCTC GGGAGCAATC CGGCTGAAGA TCAGCGTGGA GATGAAGGGA 
GAAGAGAAGG TGGCTCCACC TCACGGACAG TACACATGTT TACATGAGAA TCTCTTCCAC 
TACCTGACGG AGGTGAAGAA CAATGGAGTG GTGAAGATCC CGCAGGTGAA AGGAGATGAC 
GCCTGGAAGG TTTATTTTGA CGATGTTTCG CAGGAGATTG TGGATGAGTT CGCCATGCGC 
TTCGGTGTCG AGTCCATCTA TCAGGCCATG ACACACTTCT CATGCCTCTC GTCCAAGTAC 
ATGTGTCCTG GCGTTCCCGC GGTGATGAGT AACCTGCTTG CCAACATCAA CGCTTACTTC 
GCACACACAA CAACTGCAAC CACAAACATC TCGGCCTCCG ACCGATTCGC AGCATCCAAC 
TTTGGGAGAG AGAAGTTCGT CAAGCTCTTG GATCAGCTTC ACAACTCTCT GAGGATCGAC 
CTCTCCAAGT ACCGGGATAA CTTCCCTGCT GGAAATCCAG AGAGACTTCA TGACTTAAAA 
TCAACCGTTG ACCTGCTGAC CAGCATCACT TTCTTCAGGA TGAAGGTTCA GGAGCTTCAG 
AGTCCCCCCA GAGCCAGTAT GGTGGTTAAA GACTGCGTGA AGGCCTGTCT GGACTCCACA 
TACAAATACA TCTTTGACAA CTGCCATGAG CTCTACAACC AGCTGCTGGA CCAGGCTAAG 
AAGCAGGATC TGCCACGCGA GGAACAGGGT CCGTCCATCA AGAACCTGGA CTTCTGGCCC 
AAACTGATCA CGCTGATGGT GTCTGTTATT GATGAGGACA GAACGGCATA CACACCCATC 
ATTAACCAGT TCCCTCAAGA GTTGAATATG GGTAAAATCA GCGCTGAAAT CATGTGGAGT 
CTCTTCGCCT TGGATATGAA GTACGCGATG GAGGAACATG ACAAGCATCG TCTCTGCAAG 
AGCACCGAGT ACATGAATCT GCACTTCAAA GTCAAGTGGT TCTACAACGA GTACGTGCGC 
GATCTGCCGG CGTTTAAGGG CGTCCCTCCA GAGTATTCGC TATGGTTCGA GCCGTTCGTG 
ATCCAGTGGT TGGATGAAAA TGAAGACGTA GCGATGGATT TCCTCAACGG AGCTCTGGAG 
AGAGATAAAA AAGACGGGTT CCAGCAGACA TCTGAACACG CGCTCTTCTC CTGCTCAGTG 
GTGGACGTTT TTACGCAACT CAACCAAAGC TTTGAGATCA TCAAGAAACT GGAGTGTCCA 
AACCCTCACG CCCTCGCTCA CTTCATGCGC AGATTCGCAA AGACCATAAA TAAAGTGCTC 
ATCCAGTATG CAGCCATCAT CTCCAAAGAC TTCAGCAATC ATCTCAGCAA AGAGAAAGTG 
CCCTGTATCC TCCTCAACAA CATCCAGCAG CTCAGAGTCC AGCTGGAGAA GATGTTCGAG 
TCCATGGGAG GGAAACAGCT TGACGCAGAG GCCAGCGAGC TGCTGAAGGA GCTGCAGAAT 
AAACTCAACA CCGTTCTGGA CGAGCTCAGC GCCGTCTTCG GCTCCAGCTT TAAGCCGGTG 
ATCGAGGACT GTGTGAAGCA GATGAACCAG GAGTTGGTGC AGATGAAGGG AAACGCAGGA 
AACAAGAGCA ACGCAGCCAT GGATGCAGAG ACTGTGCTGA GACCCCTGAT GGACCTGCTG 
GACAAGAATC TCATGCTCTT CGCTAAGATC TGTGAGAAGA CGGTGCTCAA ACGGGTCCTG 
AAGGAGCTCT GGAAGATTGT GCTAAACACT ATCGAGCGGC AGATCGTCCT CCCACCGCTT 
TCAGACCAGA CGCAAGGAGC TCAGATGATC TTCAGTGCTG CGAAAGACCT CGGTCAGCTC 
TCCAAACTCA AGGATCATGT GATCCGGGAA GAAGCGCGGA GTCTCAGTCC TCGTCAGTGT 
GCCGCTATGG ATTTAGTGCT TCCTACAATC AAGCAATATT TCCATGCGGG TGGAAACGGT 
CTGAAGAAAA CCTTCCTGGA AAAGAGCCCT GATCTCAAGT CCTTGAAATA CGCCCTCAGC 
CTTTACACTC AACCCACAGA TGCCTTGATC AAGAAATATA TATGCACCCA AACCTCTCAA 
GGTCAGTCCA CAACTGGATC CTTCGGTGAA GTGGCCGTTC AGGTGGACCT GATCTCACAC 
CCTGGAACTG GAGAGCACAA AGTCAGCGTT AAAGTTGTGG GTGTAAATAA CATCAACTGG 
CAGACCAACG CCATGTTTCG GCCCTTCGTG GAGATCAACG CCATCGGTCC ACATCTGGCA 
GACAAGAAAC GCAAGTTTAG CACCAAAACC AAGAATAACA ACTGGTCTCC GAAATACAAC 
GAAACATTCC AGTATGTGCT GAGTAATGAG CACGGCCCCG AGGCCTACGA GCTCCACATC 
TCCGTGAAGG ACTACTGCTT CGCCCGAGAG GACCGAATCA TCGGCATGAC AGTCCTGCAG 
CTCCGAGAAC TGGCCGAAAA GGGCAGTCTG GGCGCCTGCT ATCCGCTCAT CAGGAGCATC 
AGCATGGACG AGACCGGCCT GACCATCATG AGAATACTCT CGCAAAGGAC CAACGACGAA 
GTGGCCAAAG AGTTCGTACG TCTCAAAACA GACACGCGGT CGGCCGAGGA AGTGTCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101884666 ( XM_009293377.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009291652.2 Danio rerio protein unc-13 homolog C-like (LOC101884666), transcript variant X2, mRNA.

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CloneID	ODa149911
Clone ID Related Accession (Same CDS sequence)	XM_009293377.3
Accession Version	XM_009293377.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 6996bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	protein unc-13 homolog C-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.7) and transcript sequence (GFIL01022384.1) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009293377.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END## ##RefSeq-Attributes-START## assembly gap :: added 5039 transcript bases to patch genome assembly gap ##RefSeq-Attributes-END##

ATGGTTTCTG CCCATTTCAA AAAGCTATCG TCTTACATTG TAAAGATTTG CAAAGGAATG 
TTTACAAAGA AACTGGCAAC TACCACCAAG AAGAAAGAGA GCAGCGAAAA CAAAAAAGAA 
CCCAAATCGA CGTCCCCTGA ATTGCACGCT CGCAACCCGA CGTTCTCCAC CACACTAAAA 
AGCACAGTTA AAAAAATCTC CAAATGCTCA TCCACACGCA ATATATCTCT TGAAGAGGAT 
GACGGCAAAA CCGACTGTTC TTCTCTCTCG CCAACATTCA GTTACCGCGT AGCAATTGCC 
AACGGACTGC CCAAAAATGC CCTGTTTCTG AACAATGAAT TTCATGAGGT CTTGTCAATT 
GATAGCGATT ACTCTGACTC TCTGAACGAG GCAAAACTTG TCCATAGATT CGATGAACAA 
AAAGCTTACA CGATGCCAGT AAGAAGGAAT CGGAAGAGTT TGATTAGTTT GGCGCCCTCC 
GATGGCAGCT CGGAGGGGGA ACGTGGAGAG CGCAGCAGTC TCCACACCCT CCGATTGGGC 
GCCTTGAAAA AGCTAAGGAA ATGGAAGAAG AGCCAAGAAT GCGTGTCCTC AGACTCGGAG 
GCCAGCAATT GGAGAAAAAC TCTTGGCATT CGAAGCAAAT CTTTGGACAG GGCCGGCCGA 
CACCAGAAAA CCACTACCCT GGAGCCAGGA TCTAGCTCCA CGGGATGCAT CAGTCAGACT 
CAGGATGTGA TGGAGATGAT TTTCAAGGAG CTTCAGGGAA TCAGTCAGAT CGAGTCCGAG 
CTCTCAGAAC TGCGAGGGCA TGTAAACGCC CTCAAAAGCT CCATCGATGA GATCTCCAGC 
AGTGTGGAGG TGGTGCAGAG TGAAATCGAG CAGCTCCGTT CAGGTTTCGT GCAGTCCAGA 
CGCGAGACTC GAGACATTCA TGATTACATC CGACAGATCA GCCAACAGAG CAACAAAGCT 
ACTCTGAGAT TTCTAAATGT GCCTGAGGAG AAGTACGAAA AAACCGAAGA ACTTATCTAT 
CAGATCCTAA AAGAGAAAAT GGGTTTCATT GATGCACGTA AGGCATTTAA AATTGAACTA 
GCCCATCGGC TGGGGCAACA AAGAGAATGT TACAATGCTA AACCACGACC AATTATAGTT 
ATATTTGCAA GCCCGCAAGA CAGAGATTTA GTTTTGAAAA AATGTTATAA GCTTAAAGGG 
ACTGGAATAT CCATCTCCAC TGACAGTCTA GCACACGACT CGAAAGAAAA GAGAGAAAAG 
CCGATAGCTT CATCTCAGAC ATACGAAAGC ATGGACATCA AGGTCTCGGC TAAAGACAAG 
GCTGAAAGTG ATGACTGGGA CTCCATGGAA AGCGACAAAG AGCTTGATGA GTTAAACAAG 
AACAAATATG CAATTGTGTC CAAACCACTT CCAAAAAGTA AATCGGACAA GAAAAAATCC 
CACGATCACA GAAGAATGGG TGATGACTCT CCATATTCTG TACACTATGC GGATAACACA 
GCCTACGATG ACCCCGACAA GCAAAGCAAG TCCTACTACT CTGACATTAC ACCTGGATGG 
CTGTCTCAGA GCGACTACTC CACTCCCAAA CTTAGCCGAT CTGAATCAGA CTGCTCTAAA 
CTTTGCCAGT CTTACTCTGA GGACTTCTCA GAGAGTCAGT ATTTCACCAG AGTCAATGGC 
TGCTCTTTGC TTTCGTCCTC AGATCAGGAG CTTTGGCAGC GTAAGCAAGA AGACATGGCG 
TCCTCCTGGT ACGCCAGTCC CCCCAGTCAA ACTCTGAGCC AAGATCGACC TTATGTGGAA 
CATAATGAAG TTGAAACCAC AGAGACTATT GACAGTGGTG TAAGTAATGG TCTTGTTTGC 
ATATCAGGAG ACAGGAGCCA TTACAGCGGC TCCCAGCTAT CGCTGCAGGG TGACCTCTCG 
CCATGGAAAG ACTGGCATCA CCTTGAACAA GGTGCTGATT CAGGTTTGGA TGCCTCAACT 
GAACAGAACA TAATCTCTGA AATCAGTAGC CCATTTGACC CAGCAGCTAA CCCTGGTTTC 
CCTGAAAAAA TTATTAAGTG TCTTGAGGTT GATTTGCAAT TTGAAGGTGA GACGTTCTTG 
ACACTAGATA GCACTCCTGA GACAGGAGAC CAAGATATAG ACATGGAGCC TGAGTTTTTA 
GAACCTGAGT TAGAACCTGA AGTTGAGGCT GTGATTGAAC CAATGATTAA ACAAGAACGA 
GAACCAATGC CACAGCCAGA ACCTGAACTA GAACCTGAAC TAGAACCTGA ACCAGAACCT 
GAACCAGAAC CTGAACAAGA ACCAGAACCA GAACCAGAAC CAGTGATTGA ACCCATTCCA 
AAACCAGTAC CAGTATCAGT ATCTAAACCT GTGGTTGAAC AGCAAACCGA AAAGAAGCCT 
AAAACCAAGA AGATCCAAGC TAAACCTGAA TCAGCTCCAC AAACTTTGCA GCGACAAGAT 
GTGAACCACC ACCTCCAGCA GAACCAGAAA TCCTCAGCTA TGTACCGGAG CCAGAGTGAG 
ATTAGAAATG AGAAGGTTGA GGATGTCCCC AAATCCTGGA GTAGCAGGCT CAGCATCGAC 
CTCAGCGAGA AAACCTTCGG CTTTGGGGGA TTTGGATCCA CCCTGCAAAG AGCAAAGTCA 
GCTTTGGATT TTGTGTGGAA TAAAGGTTCC CAGAGTACTA GCGCACCGGT TGAAGAAACC 
AGCAACACCT CGTTCATGGG CCGGTTCAGA ACCACATCCA CAGCCAATAG CTCCAGTACA 
ACCATTGATT CTGACGTCTA CACAGAACCT TTCTATTACA AGGCAGAAGA AGAGGAACAA 
CAACAGCAAC AACCTGCTGA GCAACCTGTG GACAATGAAA CGCATTATGT TGAGGTGATG 
GAACAAGTGC TAGCCAATCT GGAAAACAGA ACAAACGCAA ACGAACCCGA GGAACAGTAC 
CAAGAAGAGG AATACAATGT CTCACAGGAG TATGATCTCT CTCAAGACGC CAACCTTGTA 
CTGGAATATG ATTGCAGCCT CGATGAACAG TATGATGAGG AATACAGTGA GGACTACGAT 
GACAATCCTA TGACTGAAGA CACAGCAGAA TATGAGGTTA GTTTAGTGGA TTATGTTGAA 
GAAACCGAGG AACCGGAAGC AGAGACAGAG GAACAGGAGG AGGTCAAAGA GGAGGTGCGG 
GAGGTTCAGG AAACATCTGA AATTAAAGGA GAAGATGTGG TAAAAGAGAC CGTAGAGCAA 
GAGGATGAGA ATAAAAATGT TACAGAGGTT CCTGTGCAAG AAGCACCACC CAAAAAAAGG 
ATACGTCCCA CATTCAAAGA GGCAGCACTG AGAGCTTACA GGAAGCAGAT GGCGGAGCTG 
GAGCAGCAGA TCCTGGCAGG AGACAGCACG GCTCTGGACA CGGAGGGTCG TGCCAATATT 
CTGGACCCAG CCAAACTGGG GCTGACCGGC GGAGGGGTTG GCAGTATACT TTACGGCATT 
GACAGCATGC CAGATTTACG TCGCAAGAGG ACCATGCCTA TTGTCCGAGA CCTGGCAGGC 
ATTGCGTTTG GGCTGGTGAA CAGATCGACT CTGAACAACG AGGAGCTGAA ACTGCACGTG 
CTCAGAAAGA CCCTGCAGGC GCTGATCTAT CCCATCTCCT CCACCACGCC ACACAATTTC 
GAGGTGTGGA CGGCCACGGC GCCTACATAC TGCCACGAGT GTGAGGGTCT GCTTTGGGGA 
ATCGCTCGCC AGGGCATGAG ATGTAGCGAA TGCGGAGTCA AATGCCATGA GAAGTGCCAA 
GACCTCCTGA ACGCAGACTG TCTGCAACGT GCAGTAGAGA AGAGCTCGAA ACACGGAGCC 
GAGGACAAAA CCCAGAACAT CATCATGGCC ATGAAGGAGC GCATGAAGAT CAGGGAGAAG 
AACCGGCCCG AAGTGTTCGA GGTCATTCAG GAGATGTTCC AGCTCTCGAA AGAGGACTTT 
ATCACTCATC TGAAGACCGC CAAACAGGCT GTGCTGGAGG GAACGTCCAA GTGGTCCGCC 
AAAATCACCA TCACAGTTTT ATGTGCTCAA GGTTTGCAAG CCAAAGACAA GACGGGCTCC 
AGCGACCCCT ACGTCACGGT ACAAGTGGGA AAGACCAAAC GCAGGACCAA GACCGTGTTC 
GGCAATCTCA ACCCTGTCTG GGACGAGAAG TTCTTCTTTG AGTGTCATAA CGCCACAGAC 
CGTATAAAAG TGCGAGTGTG GGATGAAGAC GATGACATAA AGTCGAGAGT GAAGCAGCAC 
TTTAAGAAAG AGTCGGATGA CTTTCTGGGT CAAACCATCA TCGAGGTGCG GATGCTGAGC 
GGAGAGATGG ACGTCTGGTA CAACCTTGAG AAGCGGACGG ATAAATCCAT GGTCTCGGGA 
GCAATCCGGC TGAAGATCAG CGTGGAGATG AAGGGAGAAG AGAAGGTGGC TCCACCTCAC 
GGACAGTACA CATGTTTACA TGAGAATCTC TTCCACTACC TGACGGAGGT GAAGAACAAT 
GGAGTGGTGA AGATCCCGCA GGTGAAAGGA GATGACGCCT GGAAGGTTTA TTTTGACGAT 
GTTTCGCAGG AGATTGTGGA TGAGTTCGCC ATGCGCTTCG GTGTCGAGTC CATCTATCAG 
GCCATGACAC ACTTCTCATG CCTCTCGTCC AAGTACATGT GTCCTGGCGT TCCCGCGGTG 
ATGAGTAACC TGCTTGCCAA CATCAACGCT TACTTCGCAC ACACAACAAC TGCAACCACA 
AACATCTCGG CCTCCGACCG ATTCGCAGCA TCCAACTTTG GGAGAGAGAA GTTCGTCAAG 
CTCTTGGATC AGCTTCACAA CTCTCTGAGG ATCGACCTCT CCAAGTACCG GGATAACTTC 
CCTGCTGGAA ATCCAGAGAG ACTTCATGAC TTAAAATCAA CCGTTGACCT GCTGACCAGC 
ATCACTTTCT TCAGGATGAA GGTTCAGGAG CTTCAGAGTC CCCCCAGAGC CAGTATGGTG 
GTTAAAGACT GCGTGAAGGC CTGTCTGGAC TCCACATACA AATACATCTT TGACAACTGC 
CATGAGCTCT ACAACCAGCT GCTGGACCAG GCTAAGAAGC AGGATCTGCC ACGCGAGGAA 
CAGGGTCCGT CCATCAAGAA CCTGGACTTC TGGCCCAAAC TGATCACGCT GATGGTGTCT 
GTTATTGATG AGGACAGAAC GGCATACACA CCCATCATTA ACCAGTTCCC TCAAGAGTTG 
AATATGGGTA AAATCAGCGC TGAAATCATG TGGAGTCTCT TCGCCTTGGA TATGAAGTAC 
GCGATGGAGG AACATGACAA GCATCGTCTC TGCAAGAGCA CCGAGTACAT GAATCTGCAC 
TTCAAAGTCA AGTGGTTCTA CAACGAGTAC GTGCGCGATC TGCCGGCGTT TAAGGGCGTC 
CCTCCAGAGT ATTCGCTATG GTTCGAGCCG TTCGTGATCC AGTGGTTGGA TGAAAATGAA 
GACGTAGCGA TGGATTTCCT CAACGGAGCT CTGGAGAGAG ATAAAAAAGA CGGGTTCCAG 
CAGACATCTG AACACGCGCT CTTCTCCTGC TCAGTGGTGG ACGTTTTTAC GCAACTCAAC 
CAAAGCTTTG AGATCATCAA GAAACTGGAG TGTCCAAACC CTCACGCCCT CGCTCACTTC 
ATGCGCAGAT TCGCAAAGAC CATAAATAAA GTGCTCATCC AGTATGCAGC CATCATCTCC 
AAAGACTTCA GCAATCATCT CAGCAAAGAG AAAGTGCCCT GTATCCTCCT CAACAACATC 
CAGCAGCTCA GAGTCCAGCT GGAGAAGATG TTCGAGTCCA TGGGAGGGAA ACAGCTTGAC 
GCAGAGGCCA GCGAGCTGCT GAAGGAGCTG CAGAATAAAC TCAACACCGT TCTGGACGAG 
CTCAGCGCCG TCTTCGGCTC CAGCTTTAAG CCGGTGATCG AGGACTGTGT GAAGCAGATG 
AACCAGGAGT TGGTGCAGAT GAAGGGAAAC GCAGGAAACA AGAGCAACGC AGCCATGGAT 
GCAGAGACTG TGCTGAGACC CCTGATGGAC CTGCTGGACA AGAATCTCAT GCTCTTCGCT 
AAGATCTGTG AGAAGACGGT GCTCAAACGG GTCCTGAAGG AGCTCTGGAA GATTGTGCTA 
AACACTATCG AGCGGCAGAT CGTCCTCCCA CCGCTTTCAG ACCAGACGCA AGGAGCTCAG 
ATGATCTTCA GTGCTGCGAA AGACCTCGGT CAGCTCTCCA AACTCAAGGA TCATGTGATC 
CGGGAAGAAG CGCGGAGTCT CAGTCCTCGT CAGTGTGCCG CTATGGATTT AGTGCTTCCT 
ACAATCAAGC AATATTTCCA TGCGGGTGGA AACGGTCTGA AGAAAACCTT CCTGGAAAAG 
AGCCCTGATC TCAAGTCCTT GAAATACGCC CTCAGCCTTT ACACTCAACC CACAGATGCC 
TTGATCAAGA AATATATATG CACCCAAACC TCTCAAGGTC AGTCCACAAC TGGATCCTTC 
GGTGAAGTGG CCGTTCAGGT GGACCTGATC TCACACCCTG GAACTGGAGA GCACAAAGTC 
AGCGTTAAAG TTGTGGGTGT AAATAACATC AACTGGCAGA CCAACGCCAT GTTTCGGCCC 
TTCGTGGAGA TCAACGCCAT CGGTCCACAT CTGGCAGACA AGAAACGCAA GTTTAGCACC 
AAAACCAAGA ATAACAACTG GTCTCCGAAA TACAACGAAA CATTCCAGTA TGTGCTGAGT 
AATGAGCACG GCCCCGAGGC CTACGAGCTC CACATCTCCG TGAAGGACTA CTGCTTCGCC 
CGAGAGGACC GAATCATCGG CATGACAGTC CTGCAGCTCC GAGAACTGGC CGAAAAGGGC 
AGTCTGGGCG CCTGCTATCC GCTCATCAGG AGCATCAGCA TGGACGAGAC CGGCCTGACC 
ATCATGAGAA TACTCTCGCA AAGGACCAAC GACGAAGTGG CCAAAGAGTT CGTACGTCTC 
AAAACAGACA CGCGGTCGGC CGAGGAAGTG TCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291652.2
CDS	699..7694
Translation	MVSAHFKKLSSYIVKICKGMFTKKLATTTKKKESSENKKEPKSTSPELHARNPTFSTTLKSTVKKISKCSSTRNISLEEDDGKTDCSSLSPTFSYRVAIANGLPKNALFLNNEFHEVLSIDSDYSDSLNEAKLVHRFDEQKAYTMPVRRNRKSLISLAPSDGSSEGERGERSSLHTLRLGALKKLRKWKKSQECVSSDSEASNWRKTLGIRSKSLDRAGRHQKTTTLEPGSSSTGCISQTQDVMEMIFKELQGISQIESELSELRGHVNALKSSIDEISSSVEVVQSEIEQLRSGFVQSRRETRDIHDYIRQISQQSNKATLRFLNVPEEKYEKTEELIYQILKEKMGFIDARKAFKIELAHRLGQQRECYNAKPRPIIVIFASPQDRDLVLKKCYKLKGTGISISTDSLAHDSKEKREKPIASSQTYESMDIKVSAKDKAESDDWDSMESDKELDELNKNKYAIVSKPLPKSKSDKKKSHDHRRMGDDSPYSVHYADNTAYDDPDKQSKSYYSDITPGWLSQSDYSTPKLSRSESDCSKLCQSYSEDFSESQYFTRVNGCSLLSSSDQELWQRKQEDMASSWYASPPSQTLSQDRPYVEHNEVETTETIDSGVSNGLVCISGDRSHYSGSQLSLQGDLSPWKDWHHLEQGADSGLDASTEQNIISEISSPFDPAANPGFPEKIIKCLEVDLQFEGETFLTLDSTPETGDQDIDMEPEFLEPELEPEVEAVIEPMIKQEREPMPQPEPELEPELEPEPEPEPEPEQEPEPEPEPVIEPIPKPVPVSVSKPVVEQQTEKKPKTKKIQAKPESAPQTLQRQDVNHHLQQNQKSSAMYRSQSEIRNEKVEDVPKSWSSRLSIDLSEKTFGFGGFGSTLQRAKSALDFVWNKGSQSTSAPVEETSNTSFMGRFRTTSTANSSSTTIDSDVYTEPFYYKAEEEEQQQQQPAEQPVDNETHYVEVMEQVLANLENRTNANEPEEQYQEEEYNVSQEYDLSQDANLVLEYDCSLDEQYDEEYSEDYDDNPMTEDTAEYEVSLVDYVEETEEPEAETEEQEEVKEEVREVQETSEIKGEDVVKETVEQEDENKNVTEVPVQEAPPKKRIRPTFKEAALRAYRKQMAELEQQILAGDSTALDTEGRANILDPAKLGLTGGGVGSILYGIDSMPDLRRKRTMPIVRDLAGIAFGLVNRSTLNNEELKLHVLRKTLQALIYPISSTTPHNFEVWTATAPTYCHECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAVEKSSKHGAEDKTQNIIMAMKERMKIREKNRPEVFEVIQEMFQLSKEDFITHLKTAKQAVLEGTSKWSAKITITVLCAQGLQAKDKTGSSDPYVTVQVGKTKRRTKTVFGNLNPVWDEKFFFECHNATDRIKVRVWDEDDDIKSRVKQHFKKESDDFLGQTIIEVRMLSGEMDVWYNLEKRTDKSMVSGAIRLKISVEMKGEEKVAPPHGQYTCLHENLFHYLTEVKNNGVVKIPQVKGDDAWKVYFDDVSQEIVDEFAMRFGVESIYQAMTHFSCLSSKYMCPGVPAVMSNLLANINAYFAHTTTATTNISASDRFAASNFGREKFVKLLDQLHNSLRIDLSKYRDNFPAGNPERLHDLKSTVDLLTSITFFRMKVQELQSPPRASMVVKDCVKACLDSTYKYIFDNCHELYNQLLDQAKKQDLPREEQGPSIKNLDFWPKLITLMVSVIDEDRTAYTPIINQFPQELNMGKISAEIMWSLFALDMKYAMEEHDKHRLCKSTEYMNLHFKVKWFYNEYVRDLPAFKGVPPEYSLWFEPFVIQWLDENEDVAMDFLNGALERDKKDGFQQTSEHALFSCSVVDVFTQLNQSFEIIKKLECPNPHALAHFMRRFAKTINKVLIQYAAIISKDFSNHLSKEKVPCILLNNIQQLRVQLEKMFESMGGKQLDAEASELLKELQNKLNTVLDELSAVFGSSFKPVIEDCVKQMNQELVQMKGNAGNKSNAAMDAETVLRPLMDLLDKNLMLFAKICEKTVLKRVLKELWKIVLNTIERQIVLPPLSDQTQGAQMIFSAAKDLGQLSKLKDHVIREEARSLSPRQCAAMDLVLPTIKQYFHAGGNGLKKTFLEKSPDLKSLKYALSLYTQPTDALIKKYICTQTSQGQSTTGSFGEVAVQVDLISHPGTGEHKVSVKVVGVNNINWQTNAMFRPFVEINAIGPHLADKKRKFSTKTKNNNWSPKYNETFQYVLSNEHGPEAYELHISVKDYCFAREDRIIGMTVLQLRELAEKGSLGACYPLIRSISMDETGLTIMRILSQRTNDEVAKEFVRLKTDTRSAEEVS

Target ORF information:

RefSeq Version	XM_009293377.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio protein unc-13 homolog C-like (LOC101884666), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293377.3

ORF Insert Sequence:

ATGGTTTCTG CCCATTTCAA AAAGCTATCG TCTTACATTG TAAAGATTTG CAAAGGAATG 
TTTACAAAGA AACTGGCAAC TACCACCAAG AAGAAAGAGA GCAGCGAAAA CAAAAAAGAA 
CCCAAATCGA CGTCCCCTGA ATTGCACGCT CGCAACCCGA CGTTCTCCAC CACACTAAAA 
AGCACAGTTA AAAAAATCTC CAAATGCTCA TCCACACGCA ATATATCTCT TGAAGAGGAT 
GACGGCAAAA CCGACTGTTC TTCTCTCTCG CCAACATTCA GTTACCGCGT AGCAATTGCC 
AACGGACTGC CCAAAAATGC CCTGTTTCTG AACAATGAAT TTCATGAGGT CTTGTCAATT 
GATAGCGATT ACTCTGACTC TCTGAACGAG GCAAAACTTG TCCATAGATT CGATGAACAA 
AAAGCTTACA CGATGCCAGT AAGAAGGAAT CGGAAGAGTT TGATTAGTTT GGCGCCCTCC 
GATGGCAGCT CGGAGGGGGA ACGTGGAGAG CGCAGCAGTC TCCACACCCT CCGATTGGGC 
GCCTTGAAAA AGCTAAGGAA ATGGAAGAAG AGCCAAGAAT GCGTGTCCTC AGACTCGGAG 
GCCAGCAATT GGAGAAAAAC TCTTGGCATT CGAAGCAAAT CTTTGGACAG GGCCGGCCGA 
CACCAGAAAA CCACTACCCT GGAGCCAGGA TCTAGCTCCA CGGGATGCAT CAGTCAGACT 
CAGGATGTGA TGGAGATGAT TTTCAAGGAG CTTCAGGGAA TCAGTCAGAT CGAGTCCGAG 
CTCTCAGAAC TGCGAGGGCA TGTAAACGCC CTCAAAAGCT CCATCGATGA GATCTCCAGC 
AGTGTGGAGG TGGTGCAGAG TGAAATCGAG CAGCTCCGTT CAGGTTTCGT GCAGTCCAGA 
CGCGAGACTC GAGACATTCA TGATTACATC CGACAGATCA GCCAACAGAG CAACAAAGCT 
ACTCTGAGAT TTCTAAATGT GCCTGAGGAG AAGTACGAAA AAACCGAAGA ACTTATCTAT 
CAGATCCTAA AAGAGAAAAT GGGTTTCATT GATGCACGTA AGGCATTTAA AATTGAACTA 
GCCCATCGGC TGGGGCAACA AAGAGAATGT TACAATGCTA AACCACGACC AATTATAGTT 
ATATTTGCAA GCCCGCAAGA CAGAGATTTA GTTTTGAAAA AATGTTATAA GCTTAAAGGG 
ACTGGAATAT CCATCTCCAC TGACAGTCTA GCACACGACT CGAAAGAAAA GAGAGAAAAG 
CCGATAGCTT CATCTCAGAC ATACGAAAGC ATGGACATCA AGGTCTCGGC TAAAGACAAG 
GCTGAAAGTG ATGACTGGGA CTCCATGGAA AGCGACAAAG AGCTTGATGA GTTAAACAAG 
AACAAATATG CAATTGTGTC CAAACCACTT CCAAAAAGTA AATCGGACAA GAAAAAATCC 
CACGATCACA GAAGAATGGG TGATGACTCT CCATATTCTG TACACTATGC GGATAACACA 
GCCTACGATG ACCCCGACAA GCAAAGCAAG TCCTACTACT CTGACATTAC ACCTGGATGG 
CTGTCTCAGA GCGACTACTC CACTCCCAAA CTTAGCCGAT CTGAATCAGA CTGCTCTAAA 
CTTTGCCAGT CTTACTCTGA GGACTTCTCA GAGAGTCAGT ATTTCACCAG AGTCAATGGC 
TGCTCTTTGC TTTCGTCCTC AGATCAGGAG CTTTGGCAGC GTAAGCAAGA AGACATGGCG 
TCCTCCTGGT ACGCCAGTCC CCCCAGTCAA ACTCTGAGCC AAGATCGACC TTATGTGGAA 
CATAATGAAG TTGAAACCAC AGAGACTATT GACAGTGGTG TAAGTAATGG TCTTGTTTGC 
ATATCAGGAG ACAGGAGCCA TTACAGCGGC TCCCAGCTAT CGCTGCAGGG TGACCTCTCG 
CCATGGAAAG ACTGGCATCA CCTTGAACAA GGTGCTGATT CAGGTTTGGA TGCCTCAACT 
GAACAGAACA TAATCTCTGA AATCAGTAGC CCATTTGACC CAGCAGCTAA CCCTGGTTTC 
CCTGAAAAAA TTATTAAGTG TCTTGAGGTT GATTTGCAAT TTGAAGGTGA GACGTTCTTG 
ACACTAGATA GCACTCCTGA GACAGGAGAC CAAGATATAG ACATGGAGCC TGAGTTTTTA 
GAACCTGAGT TAGAACCTGA AGTTGAGGCT GTGATTGAAC CAATGATTAA ACAAGAACGA 
GAACCAATGC CACAGCCAGA ACCTGAACTA GAACCTGAAC TAGAACCTGA ACCAGAACCT 
GAACCAGAAC CTGAACAAGA ACCAGAACCA GAACCAGAAC CAGTGATTGA ACCCATTCCA 
AAACCAGTAC CAGTATCAGT ATCTAAACCT GTGGTTGAAC AGCAAACCGA AAAGAAGCCT 
AAAACCAAGA AGATCCAAGC TAAACCTGAA TCAGCTCCAC AAACTTTGCA GCGACAAGAT 
GTGAACCACC ACCTCCAGCA GAACCAGAAA TCCTCAGCTA TGTACCGGAG CCAGAGTGAG 
ATTAGAAATG AGAAGGTTGA GGATGTCCCC AAATCCTGGA GTAGCAGGCT CAGCATCGAC 
CTCAGCGAGA AAACCTTCGG CTTTGGGGGA TTTGGATCCA CCCTGCAAAG AGCAAAGTCA 
GCTTTGGATT TTGTGTGGAA TAAAGGTTCC CAGAGTACTA GCGCACCGGT TGAAGAAACC 
AGCAACACCT CGTTCATGGG CCGGTTCAGA ACCACATCCA CAGCCAATAG CTCCAGTACA 
ACCATTGATT CTGACGTCTA CACAGAACCT TTCTATTACA AGGCAGAAGA AGAGGAACAA 
CAACAGCAAC AACCTGCTGA GCAACCTGTG GACAATGAAA CGCATTATGT TGAGGTGATG 
GAACAAGTGC TAGCCAATCT GGAAAACAGA ACAAACGCAA ACGAACCCGA GGAACAGTAC 
CAAGAAGAGG AATACAATGT CTCACAGGAG TATGATCTCT CTCAAGACGC CAACCTTGTA 
CTGGAATATG ATTGCAGCCT CGATGAACAG TATGATGAGG AATACAGTGA GGACTACGAT 
GACAATCCTA TGACTGAAGA CACAGCAGAA TATGAGGTTA GTTTAGTGGA TTATGTTGAA 
GAAACCGAGG AACCGGAAGC AGAGACAGAG GAACAGGAGG AGGTCAAAGA GGAGGTGCGG 
GAGGTTCAGG AAACATCTGA AATTAAAGGA GAAGATGTGG TAAAAGAGAC CGTAGAGCAA 
GAGGATGAGA ATAAAAATGT TACAGAGGTT CCTGTGCAAG AAGCACCACC CAAAAAAAGG 
ATACGTCCCA CATTCAAAGA GGCAGCACTG AGAGCTTACA GGAAGCAGAT GGCGGAGCTG 
GAGCAGCAGA TCCTGGCAGG AGACAGCACG GCTCTGGACA CGGAGGGTCG TGCCAATATT 
CTGGACCCAG CCAAACTGGG GCTGACCGGC GGAGGGGTTG GCAGTATACT TTACGGCATT 
GACAGCATGC CAGATTTACG TCGCAAGAGG ACCATGCCTA TTGTCCGAGA CCTGGCAGGC 
ATTGCGTTTG GGCTGGTGAA CAGATCGACT CTGAACAACG AGGAGCTGAA ACTGCACGTG 
CTCAGAAAGA CCCTGCAGGC GCTGATCTAT CCCATCTCCT CCACCACGCC ACACAATTTC 
GAGGTGTGGA CGGCCACGGC GCCTACATAC TGCCACGAGT GTGAGGGTCT GCTTTGGGGA 
ATCGCTCGCC AGGGCATGAG ATGTAGCGAA TGCGGAGTCA AATGCCATGA GAAGTGCCAA 
GACCTCCTGA ACGCAGACTG TCTGCAACGT GCAGTAGAGA AGAGCTCGAA ACACGGAGCC 
GAGGACAAAA CCCAGAACAT CATCATGGCC ATGAAGGAGC GCATGAAGAT CAGGGAGAAG 
AACCGGCCCG AAGTGTTCGA GGTCATTCAG GAGATGTTCC AGCTCTCGAA AGAGGACTTT 
ATCACTCATC TGAAGACCGC CAAACAGGCT GTGCTGGAGG GAACGTCCAA GTGGTCCGCC 
AAAATCACCA TCACAGTTTT ATGTGCTCAA GGTTTGCAAG CCAAAGACAA GACGGGCTCC 
AGCGACCCCT ACGTCACGGT ACAAGTGGGA AAGACCAAAC GCAGGACCAA GACCGTGTTC 
GGCAATCTCA ACCCTGTCTG GGACGAGAAG TTCTTCTTTG AGTGTCATAA CGCCACAGAC 
CGTATAAAAG TGCGAGTGTG GGATGAAGAC GATGACATAA AGTCGAGAGT GAAGCAGCAC 
TTTAAGAAAG AGTCGGATGA CTTTCTGGGT CAAACCATCA TCGAGGTGCG GATGCTGAGC 
GGAGAGATGG ACGTCTGGTA CAACCTTGAG AAGCGGACGG ATAAATCCAT GGTCTCGGGA 
GCAATCCGGC TGAAGATCAG CGTGGAGATG AAGGGAGAAG AGAAGGTGGC TCCACCTCAC 
GGACAGTACA CATGTTTACA TGAGAATCTC TTCCACTACC TGACGGAGGT GAAGAACAAT 
GGAGTGGTGA AGATCCCGCA GGTGAAAGGA GATGACGCCT GGAAGGTTTA TTTTGACGAT 
GTTTCGCAGG AGATTGTGGA TGAGTTCGCC ATGCGCTTCG GTGTCGAGTC CATCTATCAG 
GCCATGACAC ACTTCTCATG CCTCTCGTCC AAGTACATGT GTCCTGGCGT TCCCGCGGTG 
ATGAGTAACC TGCTTGCCAA CATCAACGCT TACTTCGCAC ACACAACAAC TGCAACCACA 
AACATCTCGG CCTCCGACCG ATTCGCAGCA TCCAACTTTG GGAGAGAGAA GTTCGTCAAG 
CTCTTGGATC AGCTTCACAA CTCTCTGAGG ATCGACCTCT CCAAGTACCG GGATAACTTC 
CCTGCTGGAA ATCCAGAGAG ACTTCATGAC TTAAAATCAA CCGTTGACCT GCTGACCAGC 
ATCACTTTCT TCAGGATGAA GGTTCAGGAG CTTCAGAGTC CCCCCAGAGC CAGTATGGTG 
GTTAAAGACT GCGTGAAGGC CTGTCTGGAC TCCACATACA AATACATCTT TGACAACTGC 
CATGAGCTCT ACAACCAGCT GCTGGACCAG GCTAAGAAGC AGGATCTGCC ACGCGAGGAA 
CAGGGTCCGT CCATCAAGAA CCTGGACTTC TGGCCCAAAC TGATCACGCT GATGGTGTCT 
GTTATTGATG AGGACAGAAC GGCATACACA CCCATCATTA ACCAGTTCCC TCAAGAGTTG 
AATATGGGTA AAATCAGCGC TGAAATCATG TGGAGTCTCT TCGCCTTGGA TATGAAGTAC 
GCGATGGAGG AACATGACAA GCATCGTCTC TGCAAGAGCA CCGAGTACAT GAATCTGCAC 
TTCAAAGTCA AGTGGTTCTA CAACGAGTAC GTGCGCGATC TGCCGGCGTT TAAGGGCGTC 
CCTCCAGAGT ATTCGCTATG GTTCGAGCCG TTCGTGATCC AGTGGTTGGA TGAAAATGAA 
GACGTAGCGA TGGATTTCCT CAACGGAGCT CTGGAGAGAG ATAAAAAAGA CGGGTTCCAG 
CAGACATCTG AACACGCGCT CTTCTCCTGC TCAGTGGTGG ACGTTTTTAC GCAACTCAAC 
CAAAGCTTTG AGATCATCAA GAAACTGGAG TGTCCAAACC CTCACGCCCT CGCTCACTTC 
ATGCGCAGAT TCGCAAAGAC CATAAATAAA GTGCTCATCC AGTATGCAGC CATCATCTCC 
AAAGACTTCA GCAATCATCT CAGCAAAGAG AAAGTGCCCT GTATCCTCCT CAACAACATC 
CAGCAGCTCA GAGTCCAGCT GGAGAAGATG TTCGAGTCCA TGGGAGGGAA ACAGCTTGAC 
GCAGAGGCCA GCGAGCTGCT GAAGGAGCTG CAGAATAAAC TCAACACCGT TCTGGACGAG 
CTCAGCGCCG TCTTCGGCTC CAGCTTTAAG CCGGTGATCG AGGACTGTGT GAAGCAGATG 
AACCAGGAGT TGGTGCAGAT GAAGGGAAAC GCAGGAAACA AGAGCAACGC AGCCATGGAT 
GCAGAGACTG TGCTGAGACC CCTGATGGAC CTGCTGGACA AGAATCTCAT GCTCTTCGCT 
AAGATCTGTG AGAAGACGGT GCTCAAACGG GTCCTGAAGG AGCTCTGGAA GATTGTGCTA 
AACACTATCG AGCGGCAGAT CGTCCTCCCA CCGCTTTCAG ACCAGACGCA AGGAGCTCAG 
ATGATCTTCA GTGCTGCGAA AGACCTCGGT CAGCTCTCCA AACTCAAGGA TCATGTGATC 
CGGGAAGAAG CGCGGAGTCT CAGTCCTCGT CAGTGTGCCG CTATGGATTT AGTGCTTCCT 
ACAATCAAGC AATATTTCCA TGCGGGTGGA AACGGTCTGA AGAAAACCTT CCTGGAAAAG 
AGCCCTGATC TCAAGTCCTT GAAATACGCC CTCAGCCTTT ACACTCAACC CACAGATGCC 
TTGATCAAGA AATATATATG CACCCAAACC TCTCAAGGTC AGTCCACAAC TGGATCCTTC 
GGTGAAGTGG CCGTTCAGGT GGACCTGATC TCACACCCTG GAACTGGAGA GCACAAAGTC 
AGCGTTAAAG TTGTGGGTGT AAATAACATC AACTGGCAGA CCAACGCCAT GTTTCGGCCC 
TTCGTGGAGA TCAACGCCAT CGGTCCACAT CTGGCAGACA AGAAACGCAA GTTTAGCACC 
AAAACCAAGA ATAACAACTG GTCTCCGAAA TACAACGAAA CATTCCAGTA TGTGCTGAGT 
AATGAGCACG GCCCCGAGGC CTACGAGCTC CACATCTCCG TGAAGGACTA CTGCTTCGCC 
CGAGAGGACC GAATCATCGG CATGACAGTC CTGCAGCTCC GAGAACTGGC CGAAAAGGGC 
AGTCTGGGCG CCTGCTATCC GCTCATCAGG AGCATCAGCA TGGACGAGAC CGGCCTGACC 
ATCATGAGAA TACTCTCGCA AAGGACCAAC GACGAAGTGG CCAAAGAGTT CGTACGTCTC 
AAAACAGACA CGCGGTCGGC CGAGGAAGTG TCGTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101884666 ( XM_005170800.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005170857.2 Danio rerio protein unc-13 homolog C-like (LOC101884666), transcript variant X1, mRNA.

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CloneID	ODa51416
Clone ID Related Accession (Same CDS sequence)	XM_005170800.3
Accession Version	XM_005170800.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4206bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein unc-13 homolog C-like isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005170800.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTCTG CCCATTTCAA AAAGCTATCG TCTTACATTG TAAAGATTTG CAAAGGAATG 
TTTACAAAGA AACTGGCAAC TACCACCAAG AAGAAAGAGA GCAGCGAAAA CAAAAAAGAA 
CCCAAATCGA CGTCCCCTGA ATTGCACGCT CGCAACCCGA CGTTCTCCAC CACACTAAAA 
AGCACAGTTA AAAAAATCTC CAAATGCTCA TCCACACGCA ATATATCTCT TGAAGAGGAT 
GACGGCAAAA CCGACTGTTC TTCTCTCTCG CCAACATTCA GTTACCGCGT AGCAATTGCC 
AACGGACTGC CCAAAAATGC CCTGTTTCTG AACAATGAAT TTCATGAGGT CTTGTCAATT 
GATAGCGATT ACTCTGACTC TCTGAACGAG GCAAAACTTG TCCATAGATT CGATGAACAA 
AAAGCTTACA CGATGCCAGT AAGAAGGAAT CGGAAGAGTT TGATTAGTTT GGCGCCCTCC 
GATGGCAGCT CGGAGGGGGA ACGTGGAGAG CGCAGCAGTC TCCACACCCT CCGATTGGGC 
GCCTTGAAAA AGCTAAGGAA ATGGAAGAAG AGCCAAGAAT GCGTGTCCTC AGACTCGGAG 
GCCAGCAATT GGAGAAAAAC TCTTGGCATT CGAAGCAAAT CTTTGGACAG GGCCGGCCGA 
CACCAGAAAA CCACTACCCT GGAGCCAGGA TCTAGCTCCA CGGGATGCAT CAGTCAGACT 
CAGGATGTGA TGGAGATGAT TTTCAAGGAG CTTCAGGGAA TCAGTCAGAT CGAGTCCGAG 
CTCTCAGAAC TGCGAGGGCA TGTAAACGCC CTCAAAAGCT CCATCGATGA GATCTCCAGC 
AGTGTGGAGG TGGTGCAGAG TGAAATCGAG CAGCTCCGTT CAGGTTTCGT GCAGTCCAGA 
CGCGAGACTC GAGACATTCA TGATTACATC CGACAGATCA GCCAACAGAG CAACAAAGCT 
ACTCTGAGAT TTCTAAATGT GCCTGAGGAG AAGTACGAAA AAACCGAAGA ACTTATCTAT 
CAGATCCTAA AAGAGAAAAT GGGTTTCATT GATGCACGTA AGGCATTTAA AATTGAACTA 
GCCCATCGGC TGGGGCAACA AAGAGAATGT TACAATGCTA AACCACGACC AATTATAGTT 
ATATTTGCAA GCCCGCAAGA CAGAGATTTA GTTTTGAAAA AATGTTATAA GCTTAAAGGG 
ACTGGAATAT CCATCTCCAC TGACAGTCTA GCACACGACT CGAAAGAAAA GAGAGAAAAG 
CCGATAGCTT CATCTCAGAC ATACGAAAGC ATGGACATCA AGGTCTCGGC TAAAGACAAG 
GCTGAAAGTG ATGACTGGGA CTCCATGGAA AGCGACAAAG AGCTTGATGA GTTAAACAAG 
AACAAATATG CAATTGTGTC CAAACCACTT CCAAAAAGTA AATCGGACAA GAAAAAATCC 
CACGATCACA GAAGAATGGG TGATGACTCT CCATATTCTG TACACTATGC GGATAACACA 
GCCTACGATG ACCCCGACAA GCAAAGCAAG TCCTACTACT CTGACATTAC ACCTGGATGG 
CTGTCTCAGA GCGACTACTC CACTCCCAAA CTTAGCCGAT CTGAATCAGA CTGCTCTAAA 
CTTTGCCAGT CTTACTCTGA GGACTTCTCA GAGAGTCAGT ATTTCACCAG AGTCAATGGC 
TGCTCTTTGC TTTCGTCCTC AGATCAGGAG CTTTGGCAGC GTAAGCAAGA AGACATGGCG 
TCCTCCTGGT ACGCCAGTCC CCCCAGTCAA ACTCTGAGCC AAGATCGACC TTATGTGGAA 
CATAATGAAG TTGAAACCAC AGAGACTATT GACAGTGGTG TAAGTAATGG TCTTGTTTGC 
ATATCAGGAG ACAGGAGCCA TTACAGCGGC TCCCAGCTAT CGCTGCAGGG TGACCTCTCG 
CCATGGAAAG ACTGGCATCA CCTTGAACAA GGTGCTGATT CAGGTTTGGA TGCCTCAACT 
GAACAGAACA TAATCTCTGA AATCAGTAGC CCATTTGACC CAGCAGCTAA CCCTGGTTTC 
CCTGAAAAAA TTATTAAGTG TCTTGAGGTT GATTTGCAAT TTGAAGGTGA GACGTTCTTG 
ACACTAGATA GCACTCCTGA GACAGGAGAC CAAGATATAG ACATGGAGCC TGAGTTTTTA 
GAACCTGAGT TAGAACCTGA AGTTGAGGCT GTGATTGAAC CAATGATTAA ACAAGAACGA 
GAACCAATGC CACAGCCAGA ACCTGAACTA GAACCTGAAC TAGAACCTGA ACCAGAACCT 
GAACCAGAAC CTGAACAAGA ACCAGAACCA GAACCAGAAC CAGTGATTGA ACCCATTCCA 
AAACCAGTAC CAGTATCAGT ATCTAAACCT GTGGTTGAAC AGCAAACCGA AAAGAAGCCT 
AAAACCAAGA AGATCCAAGC TAAACCTGAA TCAGCTCCAC AAACTTTGCA GCGACAAGAT 
GTGAACCACC ACCTCCAGCA GAACCAGAAA TCCTCAGCTA TGTACCGGAG CCAGAGTGAG 
ATTAGAAATG AGAAGGTTGA GGATGTCCCC AAATCCTGGA GTAGCAGGCT CAGCATCGAC 
CTCAGCGAGA AAACCTTCGG CTTTGGGGGA TTTGGATCCA CCCTGCAAAG AGCAAAGTCA 
GCTTTGGATT TTGTGTGGAA TAAAGGTTCC CAGAGTACTA GCGCACCGGT TGAAGAAACC 
AGCAACACCT CGTTCATGGG CCGGTTCAGA ACCACATCCA CAGCCAATAG CTCCAGTACA 
ACCATTGATT CTGACGTCTA CACAGAACCT TTCTATTACA AGGCAGAAGA AGAGGAACAA 
CAACAGCAAC AACCTGCTGA GCAACCTGTG GACAATGAAA CGCATTATGT TGAGGTGATG 
GAACAAGTGC TAGCCAATCT GGAAAACAGA ACAAACGCAA ACGAACCCGA GGAACAGTAC 
CAAGAAGAGG AATACAATGT CTCACAGGAG TATGATCTCT CTCAAGACGC CAACCTTGTA 
CTGGAATATG ATTGCAGCCT CGATGAACAG TATGATGAGG AATACAGTGA GGACTACGAT 
GACAATCCTA TGACTGAAGA CACAGCAGAA TATGAGGTTA GTTTAGTGGA TTATGTTGAA 
GAAACCGAGG AACCGGAAGC AGAGACAGAG GAACAGGAGG AGGTCAAAGA GGAGGTGCGG 
GAGGTTCAGG AAACATCTGA AATTAAAGGA GAAGATGTGG TAAAAGAGAC CGTAGAGCAA 
GAGGATGAGA ATAAAAATGT TACAGAGGTT CCTGTGCAAG AAGCACCACC CAAAAAAAGG 
ATACGTCCCA CATTCAAAGA GGCAGCACTG AGAGCTTACA GGAAGCAGAT GGCGGAGCTG 
GAGCAGCAGA TCCTGGCAGG AGACAGCACG GCTCTGGACA CGGAGGGTCG TGCCAATATT 
CTGGACCCAG CCAAACTGGG GCTGACCGGC GGAGGGGTTG GCAGTATACT TTACGGCATT 
GACAGCATGC CAGATTTACG TCGCAAGAGG ACCATGCCTA TTGTCCGAGA CCTGGCTTTG 
ACATTGGCTG CTCGAAAGGC AGGCATTGCG TTTGGGCTGG TGAACAGATC GACTCTGAAC 
AACGAGGAGC TGAAACTGCA CGTGCTCAGA AAGACCCTGC AGGCGCTGAT CTATCCCATC 
TCCTCCACCA CGCCACACAA TTTCGAGGTG TGGACGGCCA CGGCGCCTAC ATACTGCCAC 
GAGTGTGAGG GTCTGCTTTG GGGAATCGCT CGCCAGGGCA TGAGATGTAG CGAATGCGGA 
GTCAAATGCC ATGAGAAGTG CCAAGACCTC CTGAACGCAG ACTGTCTGCA ACGTGCAGTA 
GAGAAGAGCT CGAAACACGG AGCCGAGGAC AAAACCCAGA ACATCATCAT GGCCATGAAG 
GAGCGCATGA AGATCAGGGA GAAGAACCGG CCCGAAGTGT TCGAGGTCAT TCAGGAGATG 
TTCCAGCTCT CGAAAGAGGA CTTTATCACT CATCTGAAGA CCGCCAAACA GGCTGTGCTG 
GAGGGAACGT CCAAGTGGTC CGCCAAAATC ACCATCACAG TTTTATGTGC TCAAGGTTTG 
CAAGCCAAAG ACAAGACGGG CTCCAGCGAC CCCTACGTCA CGGTACAAGT GGGAAAGACC 
AAACGCAGGA CCAAGACCGT GTTCGGCAAT CTCAACCCTG TCTGGGACGA GAAGTTCTTC 
TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005170857.2
CDS	680..4885
Translation	MVSAHFKKLSSYIVKICKGMFTKKLATTTKKKESSENKKEPKSTSPELHARNPTFSTTLKSTVKKISKCSSTRNISLEEDDGKTDCSSLSPTFSYRVAIANGLPKNALFLNNEFHEVLSIDSDYSDSLNEAKLVHRFDEQKAYTMPVRRNRKSLISLAPSDGSSEGERGERSSLHTLRLGALKKLRKWKKSQECVSSDSEASNWRKTLGIRSKSLDRAGRHQKTTTLEPGSSSTGCISQTQDVMEMIFKELQGISQIESELSELRGHVNALKSSIDEISSSVEVVQSEIEQLRSGFVQSRRETRDIHDYIRQISQQSNKATLRFLNVPEEKYEKTEELIYQILKEKMGFIDARKAFKIELAHRLGQQRECYNAKPRPIIVIFASPQDRDLVLKKCYKLKGTGISISTDSLAHDSKEKREKPIASSQTYESMDIKVSAKDKAESDDWDSMESDKELDELNKNKYAIVSKPLPKSKSDKKKSHDHRRMGDDSPYSVHYADNTAYDDPDKQSKSYYSDITPGWLSQSDYSTPKLSRSESDCSKLCQSYSEDFSESQYFTRVNGCSLLSSSDQELWQRKQEDMASSWYASPPSQTLSQDRPYVEHNEVETTETIDSGVSNGLVCISGDRSHYSGSQLSLQGDLSPWKDWHHLEQGADSGLDASTEQNIISEISSPFDPAANPGFPEKIIKCLEVDLQFEGETFLTLDSTPETGDQDIDMEPEFLEPELEPEVEAVIEPMIKQEREPMPQPEPELEPELEPEPEPEPEPEQEPEPEPEPVIEPIPKPVPVSVSKPVVEQQTEKKPKTKKIQAKPESAPQTLQRQDVNHHLQQNQKSSAMYRSQSEIRNEKVEDVPKSWSSRLSIDLSEKTFGFGGFGSTLQRAKSALDFVWNKGSQSTSAPVEETSNTSFMGRFRTTSTANSSSTTIDSDVYTEPFYYKAEEEEQQQQQPAEQPVDNETHYVEVMEQVLANLENRTNANEPEEQYQEEEYNVSQEYDLSQDANLVLEYDCSLDEQYDEEYSEDYDDNPMTEDTAEYEVSLVDYVEETEEPEAETEEQEEVKEEVREVQETSEIKGEDVVKETVEQEDENKNVTEVPVQEAPPKKRIRPTFKEAALRAYRKQMAELEQQILAGDSTALDTEGRANILDPAKLGLTGGGVGSILYGIDSMPDLRRKRTMPIVRDLALTLAARKAGIAFGLVNRSTLNNEELKLHVLRKTLQALIYPISSTTPHNFEVWTATAPTYCHECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAVEKSSKHGAEDKTQNIIMAMKERMKIREKNRPEVFEVIQEMFQLSKEDFITHLKTAKQAVLEGTSKWSAKITITVLCAQGLQAKDKTGSSDPYVTVQVGKTKRRTKTVFGNLNPVWDEKFFL

Target ORF information:

RefSeq Version	XM_005170800.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein unc-13 homolog C-like (LOC101884666), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005170800.3

ORF Insert Sequence:

ATGGTTTCTG CCCATTTCAA AAAGCTATCG TCTTACATTG TAAAGATTTG CAAAGGAATG 
TTTACAAAGA AACTGGCAAC TACCACCAAG AAGAAAGAGA GCAGCGAAAA CAAAAAAGAA 
CCCAAATCGA CGTCCCCTGA ATTGCACGCT CGCAACCCGA CGTTCTCCAC CACACTAAAA 
AGCACAGTTA AAAAAATCTC CAAATGCTCA TCCACACGCA ATATATCTCT TGAAGAGGAT 
GACGGCAAAA CCGACTGTTC TTCTCTCTCG CCAACATTCA GTTACCGCGT AGCAATTGCC 
AACGGACTGC CCAAAAATGC CCTGTTTCTG AACAATGAAT TTCATGAGGT CTTGTCAATT 
GATAGCGATT ACTCTGACTC TCTGAACGAG GCAAAACTTG TCCATAGATT CGATGAACAA 
AAAGCTTACA CGATGCCAGT AAGAAGGAAT CGGAAGAGTT TGATTAGTTT GGCGCCCTCC 
GATGGCAGCT CGGAGGGGGA ACGTGGAGAG CGCAGCAGTC TCCACACCCT CCGATTGGGC 
GCCTTGAAAA AGCTAAGGAA ATGGAAGAAG AGCCAAGAAT GCGTGTCCTC AGACTCGGAG 
GCCAGCAATT GGAGAAAAAC TCTTGGCATT CGAAGCAAAT CTTTGGACAG GGCCGGCCGA 
CACCAGAAAA CCACTACCCT GGAGCCAGGA TCTAGCTCCA CGGGATGCAT CAGTCAGACT 
CAGGATGTGA TGGAGATGAT TTTCAAGGAG CTTCAGGGAA TCAGTCAGAT CGAGTCCGAG 
CTCTCAGAAC TGCGAGGGCA TGTAAACGCC CTCAAAAGCT CCATCGATGA GATCTCCAGC 
AGTGTGGAGG TGGTGCAGAG TGAAATCGAG CAGCTCCGTT CAGGTTTCGT GCAGTCCAGA 
CGCGAGACTC GAGACATTCA TGATTACATC CGACAGATCA GCCAACAGAG CAACAAAGCT 
ACTCTGAGAT TTCTAAATGT GCCTGAGGAG AAGTACGAAA AAACCGAAGA ACTTATCTAT 
CAGATCCTAA AAGAGAAAAT GGGTTTCATT GATGCACGTA AGGCATTTAA AATTGAACTA 
GCCCATCGGC TGGGGCAACA AAGAGAATGT TACAATGCTA AACCACGACC AATTATAGTT 
ATATTTGCAA GCCCGCAAGA CAGAGATTTA GTTTTGAAAA AATGTTATAA GCTTAAAGGG 
ACTGGAATAT CCATCTCCAC TGACAGTCTA GCACACGACT CGAAAGAAAA GAGAGAAAAG 
CCGATAGCTT CATCTCAGAC ATACGAAAGC ATGGACATCA AGGTCTCGGC TAAAGACAAG 
GCTGAAAGTG ATGACTGGGA CTCCATGGAA AGCGACAAAG AGCTTGATGA GTTAAACAAG 
AACAAATATG CAATTGTGTC CAAACCACTT CCAAAAAGTA AATCGGACAA GAAAAAATCC 
CACGATCACA GAAGAATGGG TGATGACTCT CCATATTCTG TACACTATGC GGATAACACA 
GCCTACGATG ACCCCGACAA GCAAAGCAAG TCCTACTACT CTGACATTAC ACCTGGATGG 
CTGTCTCAGA GCGACTACTC CACTCCCAAA CTTAGCCGAT CTGAATCAGA CTGCTCTAAA 
CTTTGCCAGT CTTACTCTGA GGACTTCTCA GAGAGTCAGT ATTTCACCAG AGTCAATGGC 
TGCTCTTTGC TTTCGTCCTC AGATCAGGAG CTTTGGCAGC GTAAGCAAGA AGACATGGCG 
TCCTCCTGGT ACGCCAGTCC CCCCAGTCAA ACTCTGAGCC AAGATCGACC TTATGTGGAA 
CATAATGAAG TTGAAACCAC AGAGACTATT GACAGTGGTG TAAGTAATGG TCTTGTTTGC 
ATATCAGGAG ACAGGAGCCA TTACAGCGGC TCCCAGCTAT CGCTGCAGGG TGACCTCTCG 
CCATGGAAAG ACTGGCATCA CCTTGAACAA GGTGCTGATT CAGGTTTGGA TGCCTCAACT 
GAACAGAACA TAATCTCTGA AATCAGTAGC CCATTTGACC CAGCAGCTAA CCCTGGTTTC 
CCTGAAAAAA TTATTAAGTG TCTTGAGGTT GATTTGCAAT TTGAAGGTGA GACGTTCTTG 
ACACTAGATA GCACTCCTGA GACAGGAGAC CAAGATATAG ACATGGAGCC TGAGTTTTTA 
GAACCTGAGT TAGAACCTGA AGTTGAGGCT GTGATTGAAC CAATGATTAA ACAAGAACGA 
GAACCAATGC CACAGCCAGA ACCTGAACTA GAACCTGAAC TAGAACCTGA ACCAGAACCT 
GAACCAGAAC CTGAACAAGA ACCAGAACCA GAACCAGAAC CAGTGATTGA ACCCATTCCA 
AAACCAGTAC CAGTATCAGT ATCTAAACCT GTGGTTGAAC AGCAAACCGA AAAGAAGCCT 
AAAACCAAGA AGATCCAAGC TAAACCTGAA TCAGCTCCAC AAACTTTGCA GCGACAAGAT 
GTGAACCACC ACCTCCAGCA GAACCAGAAA TCCTCAGCTA TGTACCGGAG CCAGAGTGAG 
ATTAGAAATG AGAAGGTTGA GGATGTCCCC AAATCCTGGA GTAGCAGGCT CAGCATCGAC 
CTCAGCGAGA AAACCTTCGG CTTTGGGGGA TTTGGATCCA CCCTGCAAAG AGCAAAGTCA 
GCTTTGGATT TTGTGTGGAA TAAAGGTTCC CAGAGTACTA GCGCACCGGT TGAAGAAACC 
AGCAACACCT CGTTCATGGG CCGGTTCAGA ACCACATCCA CAGCCAATAG CTCCAGTACA 
ACCATTGATT CTGACGTCTA CACAGAACCT TTCTATTACA AGGCAGAAGA AGAGGAACAA 
CAACAGCAAC AACCTGCTGA GCAACCTGTG GACAATGAAA CGCATTATGT TGAGGTGATG 
GAACAAGTGC TAGCCAATCT GGAAAACAGA ACAAACGCAA ACGAACCCGA GGAACAGTAC 
CAAGAAGAGG AATACAATGT CTCACAGGAG TATGATCTCT CTCAAGACGC CAACCTTGTA 
CTGGAATATG ATTGCAGCCT CGATGAACAG TATGATGAGG AATACAGTGA GGACTACGAT 
GACAATCCTA TGACTGAAGA CACAGCAGAA TATGAGGTTA GTTTAGTGGA TTATGTTGAA 
GAAACCGAGG AACCGGAAGC AGAGACAGAG GAACAGGAGG AGGTCAAAGA GGAGGTGCGG 
GAGGTTCAGG AAACATCTGA AATTAAAGGA GAAGATGTGG TAAAAGAGAC CGTAGAGCAA 
GAGGATGAGA ATAAAAATGT TACAGAGGTT CCTGTGCAAG AAGCACCACC CAAAAAAAGG 
ATACGTCCCA CATTCAAAGA GGCAGCACTG AGAGCTTACA GGAAGCAGAT GGCGGAGCTG 
GAGCAGCAGA TCCTGGCAGG AGACAGCACG GCTCTGGACA CGGAGGGTCG TGCCAATATT 
CTGGACCCAG CCAAACTGGG GCTGACCGGC GGAGGGGTTG GCAGTATACT TTACGGCATT 
GACAGCATGC CAGATTTACG TCGCAAGAGG ACCATGCCTA TTGTCCGAGA CCTGGCTTTG 
ACATTGGCTG CTCGAAAGGC AGGCATTGCG TTTGGGCTGG TGAACAGATC GACTCTGAAC 
AACGAGGAGC TGAAACTGCA CGTGCTCAGA AAGACCCTGC AGGCGCTGAT CTATCCCATC 
TCCTCCACCA CGCCACACAA TTTCGAGGTG TGGACGGCCA CGGCGCCTAC ATACTGCCAC 
GAGTGTGAGG GTCTGCTTTG GGGAATCGCT CGCCAGGGCA TGAGATGTAG CGAATGCGGA 
GTCAAATGCC ATGAGAAGTG CCAAGACCTC CTGAACGCAG ACTGTCTGCA ACGTGCAGTA 
GAGAAGAGCT CGAAACACGG AGCCGAGGAC AAAACCCAGA ACATCATCAT GGCCATGAAG 
GAGCGCATGA AGATCAGGGA GAAGAACCGG CCCGAAGTGT TCGAGGTCAT TCAGGAGATG 
TTCCAGCTCT CGAAAGAGGA CTTTATCACT CATCTGAAGA CCGCCAAACA GGCTGTGCTG 
GAGGGAACGT CCAAGTGGTC CGCCAAAATC ACCATCACAG TTTTATGTGC TCAAGGTTTG 
CAAGCCAAAG ACAAGACGGG CTCCAGCGAC CCCTACGTCA CGGTACAAGT GGGAAAGACC 
AAACGCAGGA CCAAGACCGT GTTCGGCAAT CTCAACCCTG TCTGGGACGA GAAGTTCTTC 
TTGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC101884666 ( XM_009293377.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009291652.1 Danio rerio protein unc-13 homolog C-like (LOC101884666), transcript variant X2, mRNA.

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CloneID	ODa51417
Clone ID Related Accession (Same CDS sequence)	XM_009293377.2
Accession Version	XM_009293377.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4182bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	protein unc-13 homolog C-like isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007129.6) annotated using gene prediction method: Gnomon, supported by mRNA and EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009293377.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTTTCTG CCCATTTCAA AAAGCTATCG TCTTACATTG TAAAGATTTG CAAAGGAATG 
TTTACAAAGA AACTGGCAAC TACCACCAAG AAGAAAGAGA GCAGCGAAAA CAAAAAAGAA 
CCCAAATCGA CGTCCCCTGA ATTGCACGCT CGCAACCCGA CGTTCTCCAC CACACTAAAA 
AGCACAGTTA AAAAAATCTC CAAATGCTCA TCCACACGCA ATATATCTCT TGAAGAGGAT 
GACGGCAAAA CCGACTGTTC TTCTCTCTCG CCAACATTCA GTTACCGCGT AGCAATTGCC 
AACGGACTGC CCAAAAATGC CCTGTTTCTG AACAATGAAT TTCATGAGGT CTTGTCAATT 
GATAGCGATT ACTCTGACTC TCTGAACGAG GCAAAACTTG TCCATAGATT CGATGAACAA 
AAAGCTTACA CGATGCCAGT AAGAAGGAAT CGGAAGAGTT TGATTAGTTT GGCGCCCTCC 
GATGGCAGCT CGGAGGGGGA ACGTGGAGAG CGCAGCAGTC TCCACACCCT CCGATTGGGC 
GCCTTGAAAA AGCTAAGGAA ATGGAAGAAG AGCCAAGAAT GCGTGTCCTC AGACTCGGAG 
GCCAGCAATT GGAGAAAAAC TCTTGGCATT CGAAGCAAAT CTTTGGACAG GGCCGGCCGA 
CACCAGAAAA CCACTACCCT GGAGCCAGGA TCTAGCTCCA CGGGATGCAT CAGTCAGACT 
CAGGATGTGA TGGAGATGAT TTTCAAGGAG CTTCAGGGAA TCAGTCAGAT CGAGTCCGAG 
CTCTCAGAAC TGCGAGGGCA TGTAAACGCC CTCAAAAGCT CCATCGATGA GATCTCCAGC 
AGTGTGGAGG TGGTGCAGAG TGAAATCGAG CAGCTCCGTT CAGGTTTCGT GCAGTCCAGA 
CGCGAGACTC GAGACATTCA TGATTACATC CGACAGATCA GCCAACAGAG CAACAAAGCT 
ACTCTGAGAT TTCTAAATGT GCCTGAGGAG AAGTACGAAA AAACCGAAGA ACTTATCTAT 
CAGATCCTAA AAGAGAAAAT GGGTTTCATT GATGCACGTA AGGCATTTAA AATTGAACTA 
GCCCATCGGC TGGGGCAACA AAGAGAATGT TACAATGCTA AACCACGACC AATTATAGTT 
ATATTTGCAA GCCCGCAAGA CAGAGATTTA GTTTTGAAAA AATGTTATAA GCTTAAAGGG 
ACTGGAATAT CCATCTCCAC TGACAGTCTA GCACACGACT CGAAAGAAAA GAGAGAAAAG 
CCGATAGCTT CATCTCAGAC ATACGAAAGC ATGGACATCA AGGTCTCGGC TAAAGACAAG 
GCTGAAAGTG ATGACTGGGA CTCCATGGAA AGCGACAAAG AGCTTGATGA GTTAAACAAG 
AACAAATATG CAATTGTGTC CAAACCACTT CCAAAAAGTA AATCGGACAA GAAAAAATCC 
CACGATCACA GAAGAATGGG TGATGACTCT CCATATTCTG TACACTATGC GGATAACACA 
GCCTACGATG ACCCCGACAA GCAAAGCAAG TCCTACTACT CTGACATTAC ACCTGGATGG 
CTGTCTCAGA GCGACTACTC CACTCCCAAA CTTAGCCGAT CTGAATCAGA CTGCTCTAAA 
CTTTGCCAGT CTTACTCTGA GGACTTCTCA GAGAGTCAGT ATTTCACCAG AGTCAATGGC 
TGCTCTTTGC TTTCGTCCTC AGATCAGGAG CTTTGGCAGC GTAAGCAAGA AGACATGGCG 
TCCTCCTGGT ACGCCAGTCC CCCCAGTCAA ACTCTGAGCC AAGATCGACC TTATGTGGAA 
CATAATGAAG TTGAAACCAC AGAGACTATT GACAGTGGTG TAAGTAATGG TCTTGTTTGC 
ATATCAGGAG ACAGGAGCCA TTACAGCGGC TCCCAGCTAT CGCTGCAGGG TGACCTCTCG 
CCATGGAAAG ACTGGCATCA CCTTGAACAA GGTGCTGATT CAGGTTTGGA TGCCTCAACT 
GAACAGAACA TAATCTCTGA AATCAGTAGC CCATTTGACC CAGCAGCTAA CCCTGGTTTC 
CCTGAAAAAA TTATTAAGTG TCTTGAGGTT GATTTGCAAT TTGAAGGTGA GACGTTCTTG 
ACACTAGATA GCACTCCTGA GACAGGAGAC CAAGATATAG ACATGGAGCC TGAGTTTTTA 
GAACCTGAGT TAGAACCTGA AGTTGAGGCT GTGATTGAAC CAATGATTAA ACAAGAACGA 
GAACCAATGC CACAGCCAGA ACCTGAACTA GAACCTGAAC TAGAACCTGA ACCAGAACCT 
GAACCAGAAC CTGAACAAGA ACCAGAACCA GAACCAGAAC CAGTGATTGA ACCCATTCCA 
AAACCAGTAC CAGTATCAGT ATCTAAACCT GTGGTTGAAC AGCAAACCGA AAAGAAGCCT 
AAAACCAAGA AGATCCAAGC TAAACCTGAA TCAGCTCCAC AAACTTTGCA GCGACAAGAT 
GTGAACCACC ACCTCCAGCA GAACCAGAAA TCCTCAGCTA TGTACCGGAG CCAGAGTGAG 
ATTAGAAATG AGAAGGTTGA GGATGTCCCC AAATCCTGGA GTAGCAGGCT CAGCATCGAC 
CTCAGCGAGA AAACCTTCGG CTTTGGGGGA TTTGGATCCA CCCTGCAAAG AGCAAAGTCA 
GCTTTGGATT TTGTGTGGAA TAAAGGTTCC CAGAGTACTA GCGCACCGGT TGAAGAAACC 
AGCAACACCT CGTTCATGGG CCGGTTCAGA ACCACATCCA CAGCCAATAG CTCCAGTACA 
ACCATTGATT CTGACGTCTA CACAGAACCT TTCTATTACA AGGCAGAAGA AGAGGAACAA 
CAACAGCAAC AACCTGCTGA GCAACCTGTG GACAATGAAA CGCATTATGT TGAGGTGATG 
GAACAAGTGC TAGCCAATCT GGAAAACAGA ACAAACGCAA ACGAACCCGA GGAACAGTAC 
CAAGAAGAGG AATACAATGT CTCACAGGAG TATGATCTCT CTCAAGACGC CAACCTTGTA 
CTGGAATATG ATTGCAGCCT CGATGAACAG TATGATGAGG AATACAGTGA GGACTACGAT 
GACAATCCTA TGACTGAAGA CACAGCAGAA TATGAGGTTA GTTTAGTGGA TTATGTTGAA 
GAAACCGAGG AACCGGAAGC AGAGACAGAG GAACAGGAGG AGGTCAAAGA GGAGGTGCGG 
GAGGTTCAGG AAACATCTGA AATTAAAGGA GAAGATGTGG TAAAAGAGAC CGTAGAGCAA 
GAGGATGAGA ATAAAAATGT TACAGAGGTT CCTGTGCAAG AAGCACCACC CAAAAAAAGG 
ATACGTCCCA CATTCAAAGA GGCAGCACTG AGAGCTTACA GGAAGCAGAT GGCGGAGCTG 
GAGCAGCAGA TCCTGGCAGG AGACAGCACG GCTCTGGACA CGGAGGGTCG TGCCAATATT 
CTGGACCCAG CCAAACTGGG GCTGACCGGC GGAGGGGTTG GCAGTATACT TTACGGCATT 
GACAGCATGC CAGATTTACG TCGCAAGAGG ACCATGCCTA TTGTCCGAGA CCTGGCAGGC 
ATTGCGTTTG GGCTGGTGAA CAGATCGACT CTGAACAACG AGGAGCTGAA ACTGCACGTG 
CTCAGAAAGA CCCTGCAGGC GCTGATCTAT CCCATCTCCT CCACCACGCC ACACAATTTC 
GAGGTGTGGA CGGCCACGGC GCCTACATAC TGCCACGAGT GTGAGGGTCT GCTTTGGGGA 
ATCGCTCGCC AGGGCATGAG ATGTAGCGAA TGCGGAGTCA AATGCCATGA GAAGTGCCAA 
GACCTCCTGA ACGCAGACTG TCTGCAACGT GCAGTAGAGA AGAGCTCGAA ACACGGAGCC 
GAGGACAAAA CCCAGAACAT CATCATGGCC ATGAAGGAGC GCATGAAGAT CAGGGAGAAG 
AACCGGCCCG AAGTGTTCGA GGTCATTCAG GAGATGTTCC AGCTCTCGAA AGAGGACTTT 
ATCACTCATC TGAAGACCGC CAAACAGGCT GTGCTGGAGG GAACGTCCAA GTGGTCCGCC 
AAAATCACCA TCACAGTTTT ATGTGCTCAA GGTTTGCAAG CCAAAGACAA GACGGGCTCC 
AGCGACCCCT ACGTCACGGT ACAAGTGGGA AAGACCAAAC GCAGGACCAA GACCGTGTTC 
GGCAATCTCA ACCCTGTCTG GGACGAGAAG TTCTTCTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009291652.1
CDS	680..4861
Translation	MVSAHFKKLSSYIVKICKGMFTKKLATTTKKKESSENKKEPKSTSPELHARNPTFSTTLKSTVKKISKCSSTRNISLEEDDGKTDCSSLSPTFSYRVAIANGLPKNALFLNNEFHEVLSIDSDYSDSLNEAKLVHRFDEQKAYTMPVRRNRKSLISLAPSDGSSEGERGERSSLHTLRLGALKKLRKWKKSQECVSSDSEASNWRKTLGIRSKSLDRAGRHQKTTTLEPGSSSTGCISQTQDVMEMIFKELQGISQIESELSELRGHVNALKSSIDEISSSVEVVQSEIEQLRSGFVQSRRETRDIHDYIRQISQQSNKATLRFLNVPEEKYEKTEELIYQILKEKMGFIDARKAFKIELAHRLGQQRECYNAKPRPIIVIFASPQDRDLVLKKCYKLKGTGISISTDSLAHDSKEKREKPIASSQTYESMDIKVSAKDKAESDDWDSMESDKELDELNKNKYAIVSKPLPKSKSDKKKSHDHRRMGDDSPYSVHYADNTAYDDPDKQSKSYYSDITPGWLSQSDYSTPKLSRSESDCSKLCQSYSEDFSESQYFTRVNGCSLLSSSDQELWQRKQEDMASSWYASPPSQTLSQDRPYVEHNEVETTETIDSGVSNGLVCISGDRSHYSGSQLSLQGDLSPWKDWHHLEQGADSGLDASTEQNIISEISSPFDPAANPGFPEKIIKCLEVDLQFEGETFLTLDSTPETGDQDIDMEPEFLEPELEPEVEAVIEPMIKQEREPMPQPEPELEPELEPEPEPEPEPEQEPEPEPEPVIEPIPKPVPVSVSKPVVEQQTEKKPKTKKIQAKPESAPQTLQRQDVNHHLQQNQKSSAMYRSQSEIRNEKVEDVPKSWSSRLSIDLSEKTFGFGGFGSTLQRAKSALDFVWNKGSQSTSAPVEETSNTSFMGRFRTTSTANSSSTTIDSDVYTEPFYYKAEEEEQQQQQPAEQPVDNETHYVEVMEQVLANLENRTNANEPEEQYQEEEYNVSQEYDLSQDANLVLEYDCSLDEQYDEEYSEDYDDNPMTEDTAEYEVSLVDYVEETEEPEAETEEQEEVKEEVREVQETSEIKGEDVVKETVEQEDENKNVTEVPVQEAPPKKRIRPTFKEAALRAYRKQMAELEQQILAGDSTALDTEGRANILDPAKLGLTGGGVGSILYGIDSMPDLRRKRTMPIVRDLAGIAFGLVNRSTLNNEELKLHVLRKTLQALIYPISSTTPHNFEVWTATAPTYCHECEGLLWGIARQGMRCSECGVKCHEKCQDLLNADCLQRAVEKSSKHGAEDKTQNIIMAMKERMKIREKNRPEVFEVIQEMFQLSKEDFITHLKTAKQAVLEGTSKWSAKITITVLCAQGLQAKDKTGSSDPYVTVQVGKTKRRTKTVFGNLNPVWDEKFFL

Target ORF information:

RefSeq Version	XM_009293377.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio protein unc-13 homolog C-like (LOC101884666), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009293377.2

ORF Insert Sequence:

ATGGTTTCTG CCCATTTCAA AAAGCTATCG TCTTACATTG TAAAGATTTG CAAAGGAATG 
TTTACAAAGA AACTGGCAAC TACCACCAAG AAGAAAGAGA GCAGCGAAAA CAAAAAAGAA 
CCCAAATCGA CGTCCCCTGA ATTGCACGCT CGCAACCCGA CGTTCTCCAC CACACTAAAA 
AGCACAGTTA AAAAAATCTC CAAATGCTCA TCCACACGCA ATATATCTCT TGAAGAGGAT 
GACGGCAAAA CCGACTGTTC TTCTCTCTCG CCAACATTCA GTTACCGCGT AGCAATTGCC 
AACGGACTGC CCAAAAATGC CCTGTTTCTG AACAATGAAT TTCATGAGGT CTTGTCAATT 
GATAGCGATT ACTCTGACTC TCTGAACGAG GCAAAACTTG TCCATAGATT CGATGAACAA 
AAAGCTTACA CGATGCCAGT AAGAAGGAAT CGGAAGAGTT TGATTAGTTT GGCGCCCTCC 
GATGGCAGCT CGGAGGGGGA ACGTGGAGAG CGCAGCAGTC TCCACACCCT CCGATTGGGC 
GCCTTGAAAA AGCTAAGGAA ATGGAAGAAG AGCCAAGAAT GCGTGTCCTC AGACTCGGAG 
GCCAGCAATT GGAGAAAAAC TCTTGGCATT CGAAGCAAAT CTTTGGACAG GGCCGGCCGA 
CACCAGAAAA CCACTACCCT GGAGCCAGGA TCTAGCTCCA CGGGATGCAT CAGTCAGACT 
CAGGATGTGA TGGAGATGAT TTTCAAGGAG CTTCAGGGAA TCAGTCAGAT CGAGTCCGAG 
CTCTCAGAAC TGCGAGGGCA TGTAAACGCC CTCAAAAGCT CCATCGATGA GATCTCCAGC 
AGTGTGGAGG TGGTGCAGAG TGAAATCGAG CAGCTCCGTT CAGGTTTCGT GCAGTCCAGA 
CGCGAGACTC GAGACATTCA TGATTACATC CGACAGATCA GCCAACAGAG CAACAAAGCT 
ACTCTGAGAT TTCTAAATGT GCCTGAGGAG AAGTACGAAA AAACCGAAGA ACTTATCTAT 
CAGATCCTAA AAGAGAAAAT GGGTTTCATT GATGCACGTA AGGCATTTAA AATTGAACTA 
GCCCATCGGC TGGGGCAACA AAGAGAATGT TACAATGCTA AACCACGACC AATTATAGTT 
ATATTTGCAA GCCCGCAAGA CAGAGATTTA GTTTTGAAAA AATGTTATAA GCTTAAAGGG 
ACTGGAATAT CCATCTCCAC TGACAGTCTA GCACACGACT CGAAAGAAAA GAGAGAAAAG 
CCGATAGCTT CATCTCAGAC ATACGAAAGC ATGGACATCA AGGTCTCGGC TAAAGACAAG 
GCTGAAAGTG ATGACTGGGA CTCCATGGAA AGCGACAAAG AGCTTGATGA GTTAAACAAG 
AACAAATATG CAATTGTGTC CAAACCACTT CCAAAAAGTA AATCGGACAA GAAAAAATCC 
CACGATCACA GAAGAATGGG TGATGACTCT CCATATTCTG TACACTATGC GGATAACACA 
GCCTACGATG ACCCCGACAA GCAAAGCAAG TCCTACTACT CTGACATTAC ACCTGGATGG 
CTGTCTCAGA GCGACTACTC CACTCCCAAA CTTAGCCGAT CTGAATCAGA CTGCTCTAAA 
CTTTGCCAGT CTTACTCTGA GGACTTCTCA GAGAGTCAGT ATTTCACCAG AGTCAATGGC 
TGCTCTTTGC TTTCGTCCTC AGATCAGGAG CTTTGGCAGC GTAAGCAAGA AGACATGGCG 
TCCTCCTGGT ACGCCAGTCC CCCCAGTCAA ACTCTGAGCC AAGATCGACC TTATGTGGAA 
CATAATGAAG TTGAAACCAC AGAGACTATT GACAGTGGTG TAAGTAATGG TCTTGTTTGC 
ATATCAGGAG ACAGGAGCCA TTACAGCGGC TCCCAGCTAT CGCTGCAGGG TGACCTCTCG 
CCATGGAAAG ACTGGCATCA CCTTGAACAA GGTGCTGATT CAGGTTTGGA TGCCTCAACT 
GAACAGAACA TAATCTCTGA AATCAGTAGC CCATTTGACC CAGCAGCTAA CCCTGGTTTC 
CCTGAAAAAA TTATTAAGTG TCTTGAGGTT GATTTGCAAT TTGAAGGTGA GACGTTCTTG 
ACACTAGATA GCACTCCTGA GACAGGAGAC CAAGATATAG ACATGGAGCC TGAGTTTTTA 
GAACCTGAGT TAGAACCTGA AGTTGAGGCT GTGATTGAAC CAATGATTAA ACAAGAACGA 
GAACCAATGC CACAGCCAGA ACCTGAACTA GAACCTGAAC TAGAACCTGA ACCAGAACCT 
GAACCAGAAC CTGAACAAGA ACCAGAACCA GAACCAGAAC CAGTGATTGA ACCCATTCCA 
AAACCAGTAC CAGTATCAGT ATCTAAACCT GTGGTTGAAC AGCAAACCGA AAAGAAGCCT 
AAAACCAAGA AGATCCAAGC TAAACCTGAA TCAGCTCCAC AAACTTTGCA GCGACAAGAT 
GTGAACCACC ACCTCCAGCA GAACCAGAAA TCCTCAGCTA TGTACCGGAG CCAGAGTGAG 
ATTAGAAATG AGAAGGTTGA GGATGTCCCC AAATCCTGGA GTAGCAGGCT CAGCATCGAC 
CTCAGCGAGA AAACCTTCGG CTTTGGGGGA TTTGGATCCA CCCTGCAAAG AGCAAAGTCA 
GCTTTGGATT TTGTGTGGAA TAAAGGTTCC CAGAGTACTA GCGCACCGGT TGAAGAAACC 
AGCAACACCT CGTTCATGGG CCGGTTCAGA ACCACATCCA CAGCCAATAG CTCCAGTACA 
ACCATTGATT CTGACGTCTA CACAGAACCT TTCTATTACA AGGCAGAAGA AGAGGAACAA 
CAACAGCAAC AACCTGCTGA GCAACCTGTG GACAATGAAA CGCATTATGT TGAGGTGATG 
GAACAAGTGC TAGCCAATCT GGAAAACAGA ACAAACGCAA ACGAACCCGA GGAACAGTAC 
CAAGAAGAGG AATACAATGT CTCACAGGAG TATGATCTCT CTCAAGACGC CAACCTTGTA 
CTGGAATATG ATTGCAGCCT CGATGAACAG TATGATGAGG AATACAGTGA GGACTACGAT 
GACAATCCTA TGACTGAAGA CACAGCAGAA TATGAGGTTA GTTTAGTGGA TTATGTTGAA 
GAAACCGAGG AACCGGAAGC AGAGACAGAG GAACAGGAGG AGGTCAAAGA GGAGGTGCGG 
GAGGTTCAGG AAACATCTGA AATTAAAGGA GAAGATGTGG TAAAAGAGAC CGTAGAGCAA 
GAGGATGAGA ATAAAAATGT TACAGAGGTT CCTGTGCAAG AAGCACCACC CAAAAAAAGG 
ATACGTCCCA CATTCAAAGA GGCAGCACTG AGAGCTTACA GGAAGCAGAT GGCGGAGCTG 
GAGCAGCAGA TCCTGGCAGG AGACAGCACG GCTCTGGACA CGGAGGGTCG TGCCAATATT 
CTGGACCCAG CCAAACTGGG GCTGACCGGC GGAGGGGTTG GCAGTATACT TTACGGCATT 
GACAGCATGC CAGATTTACG TCGCAAGAGG ACCATGCCTA TTGTCCGAGA CCTGGCAGGC 
ATTGCGTTTG GGCTGGTGAA CAGATCGACT CTGAACAACG AGGAGCTGAA ACTGCACGTG 
CTCAGAAAGA CCCTGCAGGC GCTGATCTAT CCCATCTCCT CCACCACGCC ACACAATTTC 
GAGGTGTGGA CGGCCACGGC GCCTACATAC TGCCACGAGT GTGAGGGTCT GCTTTGGGGA 
ATCGCTCGCC AGGGCATGAG ATGTAGCGAA TGCGGAGTCA AATGCCATGA GAAGTGCCAA 
GACCTCCTGA ACGCAGACTG TCTGCAACGT GCAGTAGAGA AGAGCTCGAA ACACGGAGCC 
GAGGACAAAA CCCAGAACAT CATCATGGCC ATGAAGGAGC GCATGAAGAT CAGGGAGAAG 
AACCGGCCCG AAGTGTTCGA GGTCATTCAG GAGATGTTCC AGCTCTCGAA AGAGGACTTT 
ATCACTCATC TGAAGACCGC CAAACAGGCT GTGCTGGAGG GAACGTCCAA GTGGTCCGCC 
AAAATCACCA TCACAGTTTT ATGTGCTCAA GGTTTGCAAG CCAAAGACAA GACGGGCTCC 
AGCGACCCCT ACGTCACGGT ACAAGTGGGA AAGACCAAAC GCAGGACCAA GACCGTGTTC 
GGCAATCTCA ACCCTGTCTG GGACGAGAAG TTCTTCTTGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

Gene evolution and gene expression after whole genome duplication in fish: the PhyloFish database.
BMC genomics17368(2016 05)
Pasquier J,Cabau C,Nguyen T,Jouanno E,Severac D,Braasch I,Journot L,Pontarotti P,Klopp C,Postlethwait JH,Guiguen Y,Bobe J

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LOC101884666 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Related articles in PubMed

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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