plch2a cDNA ORF clone, Danio rerio(zebrafish)

The following plch2a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the plch2a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa143640 XM_021478169.1
Latest version!
Danio rerio phospholipase C, eta 2a (plch2a), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
ODa143641 XM_021478170.1
Latest version!
Danio rerio phospholipase C, eta 2a (plch2a), transcript variant X7, mRNA. pcDNA3.1-C-(k)DYK or customized vector 14-16 $510.30
$729.00
ODa60126 XM_017357542.2
Latest version!
Danio rerio phospholipase C, eta 2a (plch2a), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa49932 XM_009304268.3
Latest version!
Danio rerio phospholipase C, eta 2a (plch2a), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa60125 NM_001347692.1
Latest version!
Danio rerio phospholipase C, eta 2a (plch2a), mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa60125 XM_017357540.2
Latest version!
Danio rerio phospholipase C, eta 2a (plch2a), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
ODa49930 XM_009304266.3
Latest version!
Danio rerio phospholipase C, eta 2a (plch2a), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa49931 XM_009304267.3
Latest version!
Danio rerio phospholipase C, eta 2a (plch2a), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
View Old Accession Versions >>
ODa49930 XM_009304266.2 Danio rerio phospholipase C eta 2 (plch2), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $881.30
$1259.00
ODa49931 XM_009304267.2 Danio rerio phospholipase C eta 2 (plch2), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 25 $797.30
$1139.00
ODa60126 XM_017357542.1 Danio rerio phospholipase C eta 2 (plch2), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $797.30
$1139.00
ODa49932 XM_009304268.2 Danio rerio phospholipase C eta 2 (plch2), transcript variant X6, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa60125 XM_017357540.1 Danio rerio phospholipase C eta 2 (plch2), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 24-26 $881.30
$1259.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa143640
    Clone ID Related Accession (Same CDS sequence) XM_021478169.1
    Accession Version XM_021478169.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2877bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X6
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGTGTGCCG CTGTCTTGCC TGCCAGCTCG GTCACTTCTG TCGTGATGCC CCCAGCATTA 
    GGGCCAGTAC ATGCTACTAA ATCAGCAACT CCACCGGTCC GAGTACCGAG CCCAGTGGTG
    GCATCAGCCT GCTCGAAGGC ACTGCGTAAG CTGACCCTGT CAAGACTCCT TGCGGGGTTC
    TTTTGGACTG GAGAGAATAC TGTGTATGGA TCCAAATGGA GGCTTGGCTT GTTTGTGGAA
    AAATGCATGA GTTCAATGCA GACGGGCACT CAGATGGTAA AACTCCGTGG AGGATCCAAA
    GGCCTGGTGC GCTTTTTCTA CTTGGATGAA CACAAGTCCT GTATCCGCTG GCGACCGTCT
    AGGAAGAATG AGAAAGCCAA AATCTCGATC GACTCCATTC GGGAGGTTTG TGAGGGGAAG
    CAGTCTGAGA TCTTCCAGCG GTATTCAGAC GGTAGCTTTG ATCCCAACTG CTGCTTCAGC
    ATCTACTATG GCGAGCACAT GGAGTCACTT GACCTGGTGT CTGGCACAGG AGAAGAGGCT
    CGCACCTGGA TTACTGGCCT TAAGTACCTT ATGGCCGGCA TCAGCGATGA AGACAGTCTG
    GCTAAACGAC AACGCACTCG TGACCAGTGG CTGAAGCAGA CGTTTGCAGA GGCTGATAAG
    AATGGAGACG GCTGCCTCAG CATCAGTGAA GTCCTCCAGC TCCTGCACAA GCTCAACGTC
    AACCTGCCCA GGCAGAAGGT CAAGCAGATG TTTAAGGAAG CAGACACTGA TGACAACCAG
    GGTACTTTGG GATTTGAGGA GTTCTGCTCT TTCTATAAGA TGATGTCAAC GCGGCGTGAT
    CTGTACCTGC TGATGCTCAC CTACAGCAAT CATAAAGACC ACCTGGACAC TGATGACCTC
    AAGCGCTTCC TCGAGACGGA ACAAAAGATG ACAAATGTCT CTAAGGAGCA CTGTCTTGAA
    ATCGTGGCCA AGTTTGAACC TTGTCCTGAA AACCAGACAT CGGGTGTGCT GGGGATTGAT
    GGTTTTACAA ACTACATGCG CAGCCCTGCG GGGGACATCT TCAACCCTGA ACATTACGAG
    GTGAACCAAG ACATGACGCA GCCCCTCAGT GATTACTTCA TCGCTTCCTC CCACAACACG
    TATCTGATGG GAGACCAGCT CATGTCTCAG TCTCGGGTTG ATATGTACGC CTGGGTGCTG
    CAGGCCGGAT GCAGATGTGT GGAAGTGGAT TGCTGGGATG GACAGGATGG AGAACCAATC
    GTGCATCATG GCTACACCCT GACTTCCAAA ATCCTTTTTA AAGACGTCAT TGAAACCATC
    AACAAATACG CATTCGCCAA AAACCAGTAT CCAGTGATTC TGTCCATCGA GAATCACTGC
    AGCGTCCCTC AGCAAAAGAA AATGGCTCAG TATCTGACTG AAGTGTTGGG GGATAAACTG
    GACGTGTCCT CCATCCCCGC TGACCCGTCA GGACTTCTGC CTTCACCTGA AGCCCTGAAA
    GGCAAAATAC TGATCAAGGG CAAGAAACTA CCCTCAAATA TAGATGAGAA CGCAGAGGAG
    GGGGACGTGT CTGATGAAGA CAGTGCAGAT GAGATGGAGG ACGATTGTAA GCTGTTGAAT
    GGAGATACGT CTATGAACCG AAAGCAGGCG GAGAATGTGG CCAAGAAAAA ATTAGATAAC
    CTCATGAAGG AGTCAAAGAT TCGCGACCGG GAAGATCCAG ACAGTTTTAC CATCACAGCC
    CTGCCACCGT CAGGAAAACA TGACAAATCG CCACTGAAGG GAAAAGCTGA AGATAGTACC
    GACACTGGAG ATGAGGCCAA CATCGGTGGA AACAAACGTC TGGCCAGAAC CTTCATGGGC
    AGCTTTTCCA AACGCAAGAA AAAGACTAGC AAAGTGAAGA AATCCTCTAG TTTTGAGGAC
    ACCGACACCG ATCAGGAGAG CTCAAGCGGG GCATCTAAAG CACAAGCACA TCACAGCAGG
    AAGAAAAAAA CCATGAAGCT GTCAAGAGCC CTTTCTGACC TGGTATACAT GAAATCTGTG
    GGAATGCCAG ACTTTGAGTC TCAAGGTGTC ACTTGGCAGG TGTCGTCGAT GAGCGAGACC
    AAAGCTCATC AGTTAATCCA GCAGAAGCCG GTGCCGTTCA TGCGGTTCAA CCAGCGGCAG
    CTCTCTCGCG TCTACCCATC TCCTTACCGC GTGGACTCCA GCAACTTCAA CCCACAGCCC
    TTCTGGAACG CTGGCTGCCA TCTGGTTGCG CTGAATTACC AGTCAGAGGG AAGAGTGCTG
    CAGCTCAACA GAGCTAAGTT CTATTGCAAT GGCAACTGTG GTTACATTCA GAAGCCTGGC
    TGCTTGTGTG AAGGTTCCTT TAATCCAGTA GCTGAGGATC CTTTGCCAGG TCGATTGAAA
    AAGCAACTGA TTCTTAAGGT CATCAGTGGT CAGCAATTGC CCAAACCAAA AGACTCCATG
    CTGGGGGACA GAGGAGAGAT AATCGATCCG TTTGTGGAAG TGGAGATTAT TGGGCTACCG
    GTGGATTGCT GCAAAGAGCA AACCAGAGTG GTTGACGACA ATGGATTCAA TCCTATGTGG
    GAAGAAACTC TCGTCTTCAC TCTGCACATG CCAGAGAACG CTCTTATACG CTTCCTGGTG
    TGGGATCATG ATCCGATAGG CCAAGATTTC ATTGGTCAGA GGACCATCGC CTTTAACAGC
    ATGATTCCCG GTTACCGCCA TGTGTATTTG GAAGGCATGG AGGAAGCTTC TATCTTTGTT
    CATGTTGCAG TGAATGACAT CACCAGCTCG AGCAGTCAGT GGTATAAAAG GACTCTTTCA
    CAGAAACCCA AAGCAAGCAT CTCTCGACAG CCATGCTGCT GCTCTGCTCA ACCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333844.1
    CDS272..3148
    Translation

    Target ORF information:

    RefSeq Version XM_021478169.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio phospholipase C, eta 2a (plch2a), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021478169.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGTGTGCCG CTGTCTTGCC TGCCAGCTCG GTCACTTCTG TCGTGATGCC CCCAGCATTA 
    GGGCCAGTAC ATGCTACTAA ATCAGCAACT CCACCGGTCC GAGTACCGAG CCCAGTGGTG
    GCATCAGCCT GCTCGAAGGC ACTGCGTAAG CTGACCCTGT CAAGACTCCT TGCGGGGTTC
    TTTTGGACTG GAGAGAATAC TGTGTATGGA TCCAAATGGA GGCTTGGCTT GTTTGTGGAA
    AAATGCATGA GTTCAATGCA GACGGGCACT CAGATGGTAA AACTCCGTGG AGGATCCAAA
    GGCCTGGTGC GCTTTTTCTA CTTGGATGAA CACAAGTCCT GTATCCGCTG GCGACCGTCT
    AGGAAGAATG AGAAAGCCAA AATCTCGATC GACTCCATTC GGGAGGTTTG TGAGGGGAAG
    CAGTCTGAGA TCTTCCAGCG GTATTCAGAC GGTAGCTTTG ATCCCAACTG CTGCTTCAGC
    ATCTACTATG GCGAGCACAT GGAGTCACTT GACCTGGTGT CTGGCACAGG AGAAGAGGCT
    CGCACCTGGA TTACTGGCCT TAAGTACCTT ATGGCCGGCA TCAGCGATGA AGACAGTCTG
    GCTAAACGAC AACGCACTCG TGACCAGTGG CTGAAGCAGA CGTTTGCAGA GGCTGATAAG
    AATGGAGACG GCTGCCTCAG CATCAGTGAA GTCCTCCAGC TCCTGCACAA GCTCAACGTC
    AACCTGCCCA GGCAGAAGGT CAAGCAGATG TTTAAGGAAG CAGACACTGA TGACAACCAG
    GGTACTTTGG GATTTGAGGA GTTCTGCTCT TTCTATAAGA TGATGTCAAC GCGGCGTGAT
    CTGTACCTGC TGATGCTCAC CTACAGCAAT CATAAAGACC ACCTGGACAC TGATGACCTC
    AAGCGCTTCC TCGAGACGGA ACAAAAGATG ACAAATGTCT CTAAGGAGCA CTGTCTTGAA
    ATCGTGGCCA AGTTTGAACC TTGTCCTGAA AACCAGACAT CGGGTGTGCT GGGGATTGAT
    GGTTTTACAA ACTACATGCG CAGCCCTGCG GGGGACATCT TCAACCCTGA ACATTACGAG
    GTGAACCAAG ACATGACGCA GCCCCTCAGT GATTACTTCA TCGCTTCCTC CCACAACACG
    TATCTGATGG GAGACCAGCT CATGTCTCAG TCTCGGGTTG ATATGTACGC CTGGGTGCTG
    CAGGCCGGAT GCAGATGTGT GGAAGTGGAT TGCTGGGATG GACAGGATGG AGAACCAATC
    GTGCATCATG GCTACACCCT GACTTCCAAA ATCCTTTTTA AAGACGTCAT TGAAACCATC
    AACAAATACG CATTCGCCAA AAACCAGTAT CCAGTGATTC TGTCCATCGA GAATCACTGC
    AGCGTCCCTC AGCAAAAGAA AATGGCTCAG TATCTGACTG AAGTGTTGGG GGATAAACTG
    GACGTGTCCT CCATCCCCGC TGACCCGTCA GGACTTCTGC CTTCACCTGA AGCCCTGAAA
    GGCAAAATAC TGATCAAGGG CAAGAAACTA CCCTCAAATA TAGATGAGAA CGCAGAGGAG
    GGGGACGTGT CTGATGAAGA CAGTGCAGAT GAGATGGAGG ACGATTGTAA GCTGTTGAAT
    GGAGATACGT CTATGAACCG AAAGCAGGCG GAGAATGTGG CCAAGAAAAA ATTAGATAAC
    CTCATGAAGG AGTCAAAGAT TCGCGACCGG GAAGATCCAG ACAGTTTTAC CATCACAGCC
    CTGCCACCGT CAGGAAAACA TGACAAATCG CCACTGAAGG GAAAAGCTGA AGATAGTACC
    GACACTGGAG ATGAGGCCAA CATCGGTGGA AACAAACGTC TGGCCAGAAC CTTCATGGGC
    AGCTTTTCCA AACGCAAGAA AAAGACTAGC AAAGTGAAGA AATCCTCTAG TTTTGAGGAC
    ACCGACACCG ATCAGGAGAG CTCAAGCGGG GCATCTAAAG CACAAGCACA TCACAGCAGG
    AAGAAAAAAA CCATGAAGCT GTCAAGAGCC CTTTCTGACC TGGTATACAT GAAATCTGTG
    GGAATGCCAG ACTTTGAGTC TCAAGGTGTC ACTTGGCAGG TGTCGTCGAT GAGCGAGACC
    AAAGCTCATC AGTTAATCCA GCAGAAGCCG GTGCCGTTCA TGCGGTTCAA CCAGCGGCAG
    CTCTCTCGCG TCTACCCATC TCCTTACCGC GTGGACTCCA GCAACTTCAA CCCACAGCCC
    TTCTGGAACG CTGGCTGCCA TCTGGTTGCG CTGAATTACC AGTCAGAGGG AAGAGTGCTG
    CAGCTCAACA GAGCTAAGTT CTATTGCAAT GGCAACTGTG GTTACATTCA GAAGCCTGGC
    TGCTTGTGTG AAGGTTCCTT TAATCCAGTA GCTGAGGATC CTTTGCCAGG TCGATTGAAA
    AAGCAACTGA TTCTTAAGGT CATCAGTGGT CAGCAATTGC CCAAACCAAA AGACTCCATG
    CTGGGGGACA GAGGAGAGAT AATCGATCCG TTTGTGGAAG TGGAGATTAT TGGGCTACCG
    GTGGATTGCT GCAAAGAGCA AACCAGAGTG GTTGACGACA ATGGATTCAA TCCTATGTGG
    GAAGAAACTC TCGTCTTCAC TCTGCACATG CCAGAGAACG CTCTTATACG CTTCCTGGTG
    TGGGATCATG ATCCGATAGG CCAAGATTTC ATTGGTCAGA GGACCATCGC CTTTAACAGC
    ATGATTCCCG GTTACCGCCA TGTGTATTTG GAAGGCATGG AGGAAGCTTC TATCTTTGTT
    CATGTTGCAG TGAATGACAT CACCAGCTCG AGCAGTCAGT GGTATAAAAG GACTCTTTCA
    CAGAAACCCA AAGCAAGCAT CTCTCGACAG CCATGCTGCT GCTCTGCTCA ACCGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa143641
    Clone ID Related Accession (Same CDS sequence) XM_021478170.1
    Accession Version XM_021478170.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 2865bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X7
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGTGTGCCG CTGTCTTGCC TGCCAGCTCG GTCACTTCTG TCGTGATGCC CCCAGCATTA 
    GGGCCAGTAC ATGCTACTAA ATCAGCAACT CCACCGGTCC GAGTACCGAG CCCAGTGGTG
    GCATCAGCCT GCTCGAAGGC ACTGCGTAAG CTGACCCTGT CAAGACTCCT TGCGGGGTTC
    TTTTGGACTG GAGAGAATAC TGTGTATGGA TCCAAATGGA GGCTTGGCTT GTTTGTGGAA
    AAATGCATGA GTTCAATGCA GACGGGCACT CAGATGGTAA AACTCCGTGG AGGATCCAAA
    GGCCTGGTGC GCTTTTTCTA CTTGGATGAA CACAAGTCCT GTATCCGCTG GCGACCGTCT
    AGGAAGAATG AGAAAGCCAA AATCTCGATC GACTCCATTC GGGAGGTTTG TGAGGGGAAG
    CAGTCTGAGA TCTTCCAGCG GTATTCAGAC GGTAGCTTTG ATCCCAACTG CTGCTTCAGC
    ATCTACTATG GCGAGCACAT GGAGTCACTT GACCTGGTGT CTGGCACAGG AGAAGAGGCT
    CGCACCTGGA TTACTGGCCT TAAGTACCTT ATGGCCGGCA TCAGCGATGA AGACAGTCTG
    GCTAAACGAC AACGCACTCG TGACCAGTGG CTGAAGCAGA CGTTTGCAGA GGCTGATAAG
    AATGGAGACG GCTGCCTCAG CATCAGTGAA GTCCTCCAGC TCCTGCACAA GCTCAACGTC
    AACCTGCCCA GGCAGAAGGT CAAGCAGATG TTTAAGGAAG CAGACACTGA TGACAACCAG
    GGTACTTTGG GATTTGAGGA GTTCTGCTCT TTCTATAAGA TGATGTCAAC GCGGCGTGAT
    CTGTACCTGC TGATGCTCAC CTACAGCAAT CATAAAGACC ACCTGGACAC TGATGACCTC
    AAGCGCTTCC TCGAGACGGA ACAAAAGATG ACAAATGTCT CTAAGGAGCA CTGTCTTGAA
    ATCGTGGCCA AGTTTGAACC TTGTCCTGAA AACCAGACAT CGGGTGTGCT GGGGATTGAT
    GGTTTTACAA ACTACATGCG CAGCCCTGCG GGGGACATCT TCAACCCTGA ACATTACGAG
    GTGAACCAAG ACATGACGCA GCCCCTCAGT GATTACTTCA TCGCTTCCTC CCACAACACG
    TATCTGATGG GAGACCAGCT CATGTCTCAG TCTCGGGTTG ATATGTACGC CTGGGTGCTG
    CAGGCCGGAT GCAGATGTGT GGAAGTGGAT TGCTGGGATG GACAGGATGG AGAACCAATC
    GTGCATCATG GCTACACCCT GACTTCCAAA ATCCTTTTTA AAGACGTCAT TGAAACCATC
    AACAAATACG CATTCGCCAA AAACCAGTAT CCAGTGATTC TGTCCATCGA GAATCACTGC
    AGCGTCCCTC AGCAAAAGAA AATGGCTCAG TATCTGACTG AAGTGTTGGG GGATAAACTG
    GACGTGTCCT CCATCCCCGC TGACCCGTCA GGACTTCTGC CTTCACCTGA AGCCCTGAAA
    GGCAAAATAC TGATCAAGGG CAAGAAACTA CCCTCAAATA TAGATGAGAA CGCAGAGGAG
    GGGGACGTGT CTGATGAAGA CAGTGCAGAT GAGATGGAGG ACGATTGTAA GCTGTTGAAT
    GGAGATACGT CTATGAACCG AAAGCAGGCG GAGAATGTGG CCAAGAAAAA ATTAGATAAC
    CTCATGAAGG AGTCAAAGAT TCGCGACCGG GAAGATCCAG ACAGTTTTAC CATCACAGCC
    CTGCCACCGT CAGGAAAACA TGACAAATCG CCACTGAAGG GAAAAGCTGA AGATAGTACC
    GACACTGGAG ATGAGGCCAA CATCGGTGGA AACAAACGTC TGGCCAGAAC CTTCATGGGC
    AGCTTTTCCA AACGCAAGAA AAAGACTAGC AAAGTGAAGA AATCCTCTAG TTTTGAGGAC
    ACCGACACCG ATCAGGAGAG CTCAAGCGGG GCATCTAAAG CACAAGCACA TCACAGCAGG
    AAGAAAAAAA CCATGAAGCT GTCAAGAGCC CTTTCTGACC TGGTATACAT GAAATCTGTG
    GGAATGCCAG ACTTTGAGTC TCAAGGTGTC ACTTGGCAGG TGTCGTCGAT GAGCGAGACC
    AAAGCTCATC AGTTAATCCA GCAGAAGCCG GTGCCGTTCA TGCGGTTCAA CCAGCGGCAG
    CTCTCTCGCG TCTACCCATC TCCTTACCGC GTGGACTCCA GCAACTTCAA CCCACAGCCC
    TTCTGGAACG CTGGCTGCCA TCTGGTTGCG CTGAATTACC AGTCAGAGGG AAGAGTGCTG
    CAGCTCAACA GAGCTAAGTT CTATTGCAAT GGCAACTGTG GTTACATTCA GAAGCCTGGC
    TGCTTGTGTG AAGGTTCCTT TAATCCAGTA GCTGAGGATC CTTTGCCAGG TCGATTGAAA
    AAGCAACTGA TTCTTAAGGT CATCAGTGGT CAGCAATTGC CCAAACCAAA AGACTCCATG
    CTGGGGGACA GAGGAGAGAT AATCGATCCG TTTGTGGAAG TGGAGATTAT TGGGCTACCG
    GTGGATTGCT GCAAAGAGCA AACCAGAGTG GTTGACGACA ATGGATTCAA TCCTATGTGG
    GAAGAAACTC TCGTCTTCAC TCTGCACATG CCAGAGAACG CTCTTATACG CTTCCTGGTG
    TGGGATCATG ATCCGATAGG CCAAGATTTC ATTGGTCAGA GGACCATCGC CTTTAACAGC
    ATGATTCCCG GTTACCGCCA TGTGTATTTG GAAGGCATGG AGGAAGCTTC TATCTTTGTT
    CATGTTGCAG TGAATGACAT CACCAGTAAG TGCACCCATC TTAGTCCAAA AGCTGTCTAT
    AGAGGCAGGA AATTGTTAGG ATCCTCGTTC AAAACTCAGG TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021333845.1
    CDS272..3136
    Translation

    Target ORF information:

    RefSeq Version XM_021478170.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio phospholipase C, eta 2a (plch2a), transcript variant X7, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021478170.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    ATGTGTGCCG CTGTCTTGCC TGCCAGCTCG GTCACTTCTG TCGTGATGCC CCCAGCATTA 
    GGGCCAGTAC ATGCTACTAA ATCAGCAACT CCACCGGTCC GAGTACCGAG CCCAGTGGTG
    GCATCAGCCT GCTCGAAGGC ACTGCGTAAG CTGACCCTGT CAAGACTCCT TGCGGGGTTC
    TTTTGGACTG GAGAGAATAC TGTGTATGGA TCCAAATGGA GGCTTGGCTT GTTTGTGGAA
    AAATGCATGA GTTCAATGCA GACGGGCACT CAGATGGTAA AACTCCGTGG AGGATCCAAA
    GGCCTGGTGC GCTTTTTCTA CTTGGATGAA CACAAGTCCT GTATCCGCTG GCGACCGTCT
    AGGAAGAATG AGAAAGCCAA AATCTCGATC GACTCCATTC GGGAGGTTTG TGAGGGGAAG
    CAGTCTGAGA TCTTCCAGCG GTATTCAGAC GGTAGCTTTG ATCCCAACTG CTGCTTCAGC
    ATCTACTATG GCGAGCACAT GGAGTCACTT GACCTGGTGT CTGGCACAGG AGAAGAGGCT
    CGCACCTGGA TTACTGGCCT TAAGTACCTT ATGGCCGGCA TCAGCGATGA AGACAGTCTG
    GCTAAACGAC AACGCACTCG TGACCAGTGG CTGAAGCAGA CGTTTGCAGA GGCTGATAAG
    AATGGAGACG GCTGCCTCAG CATCAGTGAA GTCCTCCAGC TCCTGCACAA GCTCAACGTC
    AACCTGCCCA GGCAGAAGGT CAAGCAGATG TTTAAGGAAG CAGACACTGA TGACAACCAG
    GGTACTTTGG GATTTGAGGA GTTCTGCTCT TTCTATAAGA TGATGTCAAC GCGGCGTGAT
    CTGTACCTGC TGATGCTCAC CTACAGCAAT CATAAAGACC ACCTGGACAC TGATGACCTC
    AAGCGCTTCC TCGAGACGGA ACAAAAGATG ACAAATGTCT CTAAGGAGCA CTGTCTTGAA
    ATCGTGGCCA AGTTTGAACC TTGTCCTGAA AACCAGACAT CGGGTGTGCT GGGGATTGAT
    GGTTTTACAA ACTACATGCG CAGCCCTGCG GGGGACATCT TCAACCCTGA ACATTACGAG
    GTGAACCAAG ACATGACGCA GCCCCTCAGT GATTACTTCA TCGCTTCCTC CCACAACACG
    TATCTGATGG GAGACCAGCT CATGTCTCAG TCTCGGGTTG ATATGTACGC CTGGGTGCTG
    CAGGCCGGAT GCAGATGTGT GGAAGTGGAT TGCTGGGATG GACAGGATGG AGAACCAATC
    GTGCATCATG GCTACACCCT GACTTCCAAA ATCCTTTTTA AAGACGTCAT TGAAACCATC
    AACAAATACG CATTCGCCAA AAACCAGTAT CCAGTGATTC TGTCCATCGA GAATCACTGC
    AGCGTCCCTC AGCAAAAGAA AATGGCTCAG TATCTGACTG AAGTGTTGGG GGATAAACTG
    GACGTGTCCT CCATCCCCGC TGACCCGTCA GGACTTCTGC CTTCACCTGA AGCCCTGAAA
    GGCAAAATAC TGATCAAGGG CAAGAAACTA CCCTCAAATA TAGATGAGAA CGCAGAGGAG
    GGGGACGTGT CTGATGAAGA CAGTGCAGAT GAGATGGAGG ACGATTGTAA GCTGTTGAAT
    GGAGATACGT CTATGAACCG AAAGCAGGCG GAGAATGTGG CCAAGAAAAA ATTAGATAAC
    CTCATGAAGG AGTCAAAGAT TCGCGACCGG GAAGATCCAG ACAGTTTTAC CATCACAGCC
    CTGCCACCGT CAGGAAAACA TGACAAATCG CCACTGAAGG GAAAAGCTGA AGATAGTACC
    GACACTGGAG ATGAGGCCAA CATCGGTGGA AACAAACGTC TGGCCAGAAC CTTCATGGGC
    AGCTTTTCCA AACGCAAGAA AAAGACTAGC AAAGTGAAGA AATCCTCTAG TTTTGAGGAC
    ACCGACACCG ATCAGGAGAG CTCAAGCGGG GCATCTAAAG CACAAGCACA TCACAGCAGG
    AAGAAAAAAA CCATGAAGCT GTCAAGAGCC CTTTCTGACC TGGTATACAT GAAATCTGTG
    GGAATGCCAG ACTTTGAGTC TCAAGGTGTC ACTTGGCAGG TGTCGTCGAT GAGCGAGACC
    AAAGCTCATC AGTTAATCCA GCAGAAGCCG GTGCCGTTCA TGCGGTTCAA CCAGCGGCAG
    CTCTCTCGCG TCTACCCATC TCCTTACCGC GTGGACTCCA GCAACTTCAA CCCACAGCCC
    TTCTGGAACG CTGGCTGCCA TCTGGTTGCG CTGAATTACC AGTCAGAGGG AAGAGTGCTG
    CAGCTCAACA GAGCTAAGTT CTATTGCAAT GGCAACTGTG GTTACATTCA GAAGCCTGGC
    TGCTTGTGTG AAGGTTCCTT TAATCCAGTA GCTGAGGATC CTTTGCCAGG TCGATTGAAA
    AAGCAACTGA TTCTTAAGGT CATCAGTGGT CAGCAATTGC CCAAACCAAA AGACTCCATG
    CTGGGGGACA GAGGAGAGAT AATCGATCCG TTTGTGGAAG TGGAGATTAT TGGGCTACCG
    GTGGATTGCT GCAAAGAGCA AACCAGAGTG GTTGACGACA ATGGATTCAA TCCTATGTGG
    GAAGAAACTC TCGTCTTCAC TCTGCACATG CCAGAGAACG CTCTTATACG CTTCCTGGTG
    TGGGATCATG ATCCGATAGG CCAAGATTTC ATTGGTCAGA GGACCATCGC CTTTAACAGC
    ATGATTCCCG GTTACCGCCA TGTGTATTTG GAAGGCATGG AGGAAGCTTC TATCTTTGTT
    CATGTTGCAG TGAATGACAT CACCAGTAAG TGCACCCATC TTAGTCCAAA AGCTGTCTAT
    AGAGGCAGGA AATTGTTAGG ATCCTCGTTC AAAACTCAGG TGTAG

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa60126
    Clone ID Related Accession (Same CDS sequence) XM_017357542.1 , XM_017357542.2
    Accession Version XM_017357542.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4473bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAGAACA TCGGAGCTCT GCCTCAGAAA CACATGGAAA AATGCATGAG TTCAATGCAG 
    ACGGGCACTC AGATGGTAAA ACTCCGTGGA GGATCCAAAG GCCTGGTGCG CTTTTTCTAC
    TTGGATGAAC ACAAGTCCTG TATCCGCTGG CGACCGTCTA GGAAGAATGA GAAAGCCAAA
    ATCTCGATCG ACTCCATTCG GGAGGTTTGT GAGGGGAAGC AGTCTGAGAT CTTCCAGCGG
    TATTCAGACG GTAGCTTTGA TCCCAACTGC TGCTTCAGCA TCTACTATGG CGAGCACATG
    GAGTCACTTG ACCTGGTGTC TGGCACAGGA GAAGAGGCTC GCACCTGGAT TACTGGCCTT
    AAGTACCTTA TGGCCGGCAT CAGCGATGAA GACAGTCTGG CTAAACGACA ACGCACTCGT
    GACCAGTGGC TGAAGCAGAC GTTTGCAGAG GCTGATAAGA ATGGAGACGG CTGCCTCAGC
    ATCAGTGAAG TCCTCCAGCT CCTGCACAAG CTCAACGTCA ACCTGCCCAG GCAGAAGGTC
    AAGCAGATGT TTAAGGAAGC AGACACTGAT GACAACCAGG GTACTTTGGG ATTTGAGGAG
    TTCTGCTCTT TCTATAAGAT GATGTCAACG CGGCGTGATC TGTACCTGCT GATGCTCACC
    TACAGCAATC ATAAAGACCA CCTGGACACT GATGACCTCA AGCGCTTCCT CGAGACGGAA
    CAAAAGATGA CAAATGTCTC TAAGGAGCAC TGTCTTGAAA TCGTGGCCAA GTTTGAACCT
    TGTCCTGAAA ACCAGACATC GGGTGTGCTG GGGATTGATG GTTTTACAAA CTACATGCGC
    AGCCCTGCGG GGGACATCTT CAACCCTGAA CATTACGAGG TGAACCAAGA CATGACGCAG
    CCCCTCAGTG ATTACTTCAT CGCTTCCTCC CACAACACGT ATCTGATGGG AGACCAGCTC
    ATGTCTCAGT CTCGGGTTGA TATGTACGCC TGGGTGCTGC AGGCCGGATG CAGATGTGTG
    GAAGTGGATT GCTGGGATGG ACAGGATGGA GAACCAATCG TGCATCATGG CTACACCCTG
    ACTTCCAAAA TCCTTTTTAA AGACGTCATT GAAACCATCA ACAAATACGC ATTCGCCAAA
    AACCAGTATC CAGTGATTCT GTCCATCGAG AATCACTGCA GCGTCCCTCA GCAAAAGAAA
    ATGGCTCAGT ATCTGACTGA AGTGTTGGGG GATAAACTGG ACGTGTCCTC CATCCCCGCT
    GACCCGTCAG GACTTCTGCC TTCACCTGAA GCCCTGAAAG GCAAAATACT GATCAAGGGC
    AAGAAACTAC CCTCAAATAT AGATGAGAAC GCAGAGGAGG GGGACGTGTC TGATGAAGAC
    AGTGCAGATG AGATGGAGGA CGATTGTAAG CTGTTGAATG GAGATACGTC TATGAACCGA
    AAGCAGGCGG AGAATGTGGC CAAGAAAAAA TTAGATAACC TCATGAAGGA GTCAAAGATT
    CGCGACCGGG AAGATCCAGA CAGTTTTACC ATCACAGCCC TGCCACCGTC AGGAAAACAT
    GACAAATCGC CACTGAAGGG AAAAGCTGAA GATAGTACCG ACACTGGAGA TGAGGCCAAC
    ATCGGTGGAA ACAAACGTCT GGCCAGAACC TTCATGGGCA GCTTTTCCAA ACGCAAGAAA
    AAGACTAGCA AAGTGAAGAA ATCCTCTAGT TTTGAGGACA CCGACACCGA TCAGGAGAGC
    TCAAGCGGGG CATCTAAAGC ACAAGCACAT CACAGCAGGA AGAAAAAAAC CATGAAGCTG
    TCAAGAGCCC TTTCTGACCT GGTATACATG AAATCTGTGG GAATGCCAGA CTTTGAGTCT
    CAAGGTGTCA CTTGGCAGGT GTCGTCGATG AGCGAGACCA AAGCTCATCA GTTAATCCAG
    CAGAAGCCGG TGCCGTTCAT GCGGTTCAAC CAGCGGCAGC TCTCTCGCGT CTACCCATCT
    CCTTACCGCG TGGACTCCAG CAACTTCAAC CCACAGCCCT TCTGGAACGC TGGCTGCCAT
    CTGGTTGCGC TGAATTACCA GTCAGAGGGA AGAGTGCTGC AGCTCAACAG AGCTAAGTTC
    TATTGCAATG GCAACTGTGG TTACATTCAG AAGCCTGGCT GCTTGTGTGA AGGTTCCTTT
    AATCCAGTAG CTGAGGATCC TTTGCCAGGT CGATTGAAAA AGCAACTGAT TCTTAAGGTC
    ATCAGTGGTC AGCAATTGCC CAAACCAAAA GACTCCATGC TGGGGGACAG AGGAGAGATA
    ATCGATCCGT TTGTGGAAGT GGAGATTATT GGGCTACCGG TGGATTGCTG CAAAGAGCAA
    ACCAGAGTGG TTGACGACAA TGGATTCAAT CCTATGTGGG AAGAAACTCT CGTCTTCACT
    CTGCACATGC CAGAGAACGC TCTTATACGC TTCCTGGTGT GGGATCATGA TCCGATAGGC
    CAAGATTTCA TTGGTCAGAG GACCATCGCC TTTAACAGCA TGATTCCCGG TTACCGCCAT
    GTGTATTTGG AAGGCATGGA GGAAGCTTCT ATCTTTGTTC ATGTTGCAGT GAATGACATC
    ACCAGTAAGG CTCGAGCAGT CAGTGGTATA AAAGGACTCT TTCACAGAAA CCCAAAGCAA
    GCATCTCTCG ACAGCCATGC TGCTGCTCTG CTCAACCGTA AGAGTACCTT CGGTGCTCAC
    CTCCTGCGCC GCACAGCCAG TGCCCCCACC AAGGGCCAAC CCAAAGTCAA GAGGGGCTTC
    CCCGAGATCT CCATTGACAC AAAAGATTGC AGCTCGGACG GCGCCAGCGA GGAGCGGGAA
    TCTGAAGAGG GGCCTCTTGT CCACCACAAT GGAGATACTG CGTCTGCAAA GTCATGGGGT
    CAAGGCACCA ACGGGCAGCT TCACCCTGAT GACAGAAAAG AAAATTCTGA AGTGCAGAGT
    CCAACTCCAT CTCTGAACGC CTCATGTCTG GAATCCATCA CTGAGAACTT TCAGGAGAAC
    AGCCCTTCGA CCTCAACGCA AAGTCCTGTG GATACCTCAC CGCATTGTCC CTTGTCCTCA
    CCATCCACAG CAATAACGGC ATCTTCCAAC GGGGAAAATC TCAGCCCTGT GAAAAATAAC
    GAGGACATCC TGAAATCATT TGCACTAACC CAATCTACGA GTGGTAAGGA TTCTGGGAAC
    AGTCCTGTAA ACTCATCAAT TTCCACTAAT TACCTTGACA TTGGATGCGA CACGCCAACT
    TTTCCCACTT TCCATCGGGC GGATGGAGAG GTTGCACAAG AGACAGTCAT CAATCAAACA
    GGACTGACTC TAGAGATAGA TGCTGGGAAG GAACCAACTG CCATTCAGAG CTCTCAGAGG
    ATTCAGGCAT TGAAGGCAGT GTTTGACAGA GACATTGGAC GAAGCATGCA AACAGGACTG
    AGGACCCCTG TGAGAAGGTC CAAAAGTGAA GGACAAATGA AAAAGGCAGA CGGCCCTTCG
    GTGGTGCCAG AAGTTTGTAC TGACGCAACT TTAAACGACA GGCTCTGGAG CAAACTTGAC
    CCCAGCAGCC ATCGGGATAG TGTGTCATCG TCTTCCAGCA TTTCGTCCAA TGACACTGTG
    ATTGATCTGT CCTTGCCCAA CCTTTCCAGG AAAAGCCTTA CCTGTCTTAG CGCAGCCCGG
    GATTATTCAG ACAAGCAGGC TTCGTTGACC AACAACCATC GGTCGGTCAT TGCAGGATGT
    GGAGTCTCGC TTGTCAGCAA GAGCAAGTCC AACCCCAACC TGCGGGATGG CGAGATGTGC
    AATACAGATG ACGATTTGCA ACCCAGACCT CTGGTAGCCG ATAAAGACCC CATCCGACCC
    ATGCAAAGGC GACACACGTG GAGCAGGTTG TACGTTGAAG GCCTAAAGCA GTCATCGACA
    CTTGCTGCTT CTGCAGCCTC CAAGAAGTCA ACTACCCTTT CAGCAAAAGA CATCGACTCA
    AAGTCCAAAA GTCTGGGAGA TCTCACTTCA GACGATATCA TCTGTAACTT CGAGAGCAAG
    TATCGCAGCA TTAGCCGCAG TTTCATCGTG AGGCCAAGTC GCCAACATGG GAAAACTCTG
    AGGAAACCCC AAAATGATTT CACTGAGCAG CTGAAGAGGC TCACTGACGT GGAACCCCTT
    ACCAGCAAGG ACTTTGCTCA GTGCCGGGCT GACTCAGAAG CAGGACAGGA AGAAGAGGAG
    ATGCCTCCTT TACGGAGGAG TTCATCACGG AGCCAGAGCC GCGTGAGATA CATCGCTAAC
    CGTGCCAGGC AGGCCCAGGA AAGGCAACGG CTACAAAGCA TAACCCGGAT GTCTGGGAGC
    CCCATCGAAG AGCGTGGGAA CCCTGAAGGA GCTTGTAGTG TACCCCATAG TCCTTGCACT
    TGCCTGGATC TTTTAAGCCA GCTTCCACCA GGACCACCCC AACAAAGCCC ACTGAGCCCG
    GACAATGAGG TCTTCTTTAT GCTCAGGCTG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017213031.1
    CDS16..4488
    Translation

    Target ORF information:

    RefSeq Version XM_017357542.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio phospholipase C eta 2 (plch2), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017357542.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAGAACA TCGGAGCTCT GCCTCAGAAA CACATGGAAA AATGCATGAG TTCAATGCAG 
    ACGGGCACTC AGATGGTAAA ACTCCGTGGA GGATCCAAAG GCCTGGTGCG CTTTTTCTAC
    TTGGATGAAC ACAAGTCCTG TATCCGCTGG CGACCGTCTA GGAAGAATGA GAAAGCCAAA
    ATCTCGATCG ACTCCATTCG GGAGGTTTGT GAGGGGAAGC AGTCTGAGAT CTTCCAGCGG
    TATTCAGACG GTAGCTTTGA TCCCAACTGC TGCTTCAGCA TCTACTATGG CGAGCACATG
    GAGTCACTTG ACCTGGTGTC TGGCACAGGA GAAGAGGCTC GCACCTGGAT TACTGGCCTT
    AAGTACCTTA TGGCCGGCAT CAGCGATGAA GACAGTCTGG CTAAACGACA ACGCACTCGT
    GACCAGTGGC TGAAGCAGAC GTTTGCAGAG GCTGATAAGA ATGGAGACGG CTGCCTCAGC
    ATCAGTGAAG TCCTCCAGCT CCTGCACAAG CTCAACGTCA ACCTGCCCAG GCAGAAGGTC
    AAGCAGATGT TTAAGGAAGC AGACACTGAT GACAACCAGG GTACTTTGGG ATTTGAGGAG
    TTCTGCTCTT TCTATAAGAT GATGTCAACG CGGCGTGATC TGTACCTGCT GATGCTCACC
    TACAGCAATC ATAAAGACCA CCTGGACACT GATGACCTCA AGCGCTTCCT CGAGACGGAA
    CAAAAGATGA CAAATGTCTC TAAGGAGCAC TGTCTTGAAA TCGTGGCCAA GTTTGAACCT
    TGTCCTGAAA ACCAGACATC GGGTGTGCTG GGGATTGATG GTTTTACAAA CTACATGCGC
    AGCCCTGCGG GGGACATCTT CAACCCTGAA CATTACGAGG TGAACCAAGA CATGACGCAG
    CCCCTCAGTG ATTACTTCAT CGCTTCCTCC CACAACACGT ATCTGATGGG AGACCAGCTC
    ATGTCTCAGT CTCGGGTTGA TATGTACGCC TGGGTGCTGC AGGCCGGATG CAGATGTGTG
    GAAGTGGATT GCTGGGATGG ACAGGATGGA GAACCAATCG TGCATCATGG CTACACCCTG
    ACTTCCAAAA TCCTTTTTAA AGACGTCATT GAAACCATCA ACAAATACGC ATTCGCCAAA
    AACCAGTATC CAGTGATTCT GTCCATCGAG AATCACTGCA GCGTCCCTCA GCAAAAGAAA
    ATGGCTCAGT ATCTGACTGA AGTGTTGGGG GATAAACTGG ACGTGTCCTC CATCCCCGCT
    GACCCGTCAG GACTTCTGCC TTCACCTGAA GCCCTGAAAG GCAAAATACT GATCAAGGGC
    AAGAAACTAC CCTCAAATAT AGATGAGAAC GCAGAGGAGG GGGACGTGTC TGATGAAGAC
    AGTGCAGATG AGATGGAGGA CGATTGTAAG CTGTTGAATG GAGATACGTC TATGAACCGA
    AAGCAGGCGG AGAATGTGGC CAAGAAAAAA TTAGATAACC TCATGAAGGA GTCAAAGATT
    CGCGACCGGG AAGATCCAGA CAGTTTTACC ATCACAGCCC TGCCACCGTC AGGAAAACAT
    GACAAATCGC CACTGAAGGG AAAAGCTGAA GATAGTACCG ACACTGGAGA TGAGGCCAAC
    ATCGGTGGAA ACAAACGTCT GGCCAGAACC TTCATGGGCA GCTTTTCCAA ACGCAAGAAA
    AAGACTAGCA AAGTGAAGAA ATCCTCTAGT TTTGAGGACA CCGACACCGA TCAGGAGAGC
    TCAAGCGGGG CATCTAAAGC ACAAGCACAT CACAGCAGGA AGAAAAAAAC CATGAAGCTG
    TCAAGAGCCC TTTCTGACCT GGTATACATG AAATCTGTGG GAATGCCAGA CTTTGAGTCT
    CAAGGTGTCA CTTGGCAGGT GTCGTCGATG AGCGAGACCA AAGCTCATCA GTTAATCCAG
    CAGAAGCCGG TGCCGTTCAT GCGGTTCAAC CAGCGGCAGC TCTCTCGCGT CTACCCATCT
    CCTTACCGCG TGGACTCCAG CAACTTCAAC CCACAGCCCT TCTGGAACGC TGGCTGCCAT
    CTGGTTGCGC TGAATTACCA GTCAGAGGGA AGAGTGCTGC AGCTCAACAG AGCTAAGTTC
    TATTGCAATG GCAACTGTGG TTACATTCAG AAGCCTGGCT GCTTGTGTGA AGGTTCCTTT
    AATCCAGTAG CTGAGGATCC TTTGCCAGGT CGATTGAAAA AGCAACTGAT TCTTAAGGTC
    ATCAGTGGTC AGCAATTGCC CAAACCAAAA GACTCCATGC TGGGGGACAG AGGAGAGATA
    ATCGATCCGT TTGTGGAAGT GGAGATTATT GGGCTACCGG TGGATTGCTG CAAAGAGCAA
    ACCAGAGTGG TTGACGACAA TGGATTCAAT CCTATGTGGG AAGAAACTCT CGTCTTCACT
    CTGCACATGC CAGAGAACGC TCTTATACGC TTCCTGGTGT GGGATCATGA TCCGATAGGC
    CAAGATTTCA TTGGTCAGAG GACCATCGCC TTTAACAGCA TGATTCCCGG TTACCGCCAT
    GTGTATTTGG AAGGCATGGA GGAAGCTTCT ATCTTTGTTC ATGTTGCAGT GAATGACATC
    ACCAGTAAGG CTCGAGCAGT CAGTGGTATA AAAGGACTCT TTCACAGAAA CCCAAAGCAA
    GCATCTCTCG ACAGCCATGC TGCTGCTCTG CTCAACCGTA AGAGTACCTT CGGTGCTCAC
    CTCCTGCGCC GCACAGCCAG TGCCCCCACC AAGGGCCAAC CCAAAGTCAA GAGGGGCTTC
    CCCGAGATCT CCATTGACAC AAAAGATTGC AGCTCGGACG GCGCCAGCGA GGAGCGGGAA
    TCTGAAGAGG GGCCTCTTGT CCACCACAAT GGAGATACTG CGTCTGCAAA GTCATGGGGT
    CAAGGCACCA ACGGGCAGCT TCACCCTGAT GACAGAAAAG AAAATTCTGA AGTGCAGAGT
    CCAACTCCAT CTCTGAACGC CTCATGTCTG GAATCCATCA CTGAGAACTT TCAGGAGAAC
    AGCCCTTCGA CCTCAACGCA AAGTCCTGTG GATACCTCAC CGCATTGTCC CTTGTCCTCA
    CCATCCACAG CAATAACGGC ATCTTCCAAC GGGGAAAATC TCAGCCCTGT GAAAAATAAC
    GAGGACATCC TGAAATCATT TGCACTAACC CAATCTACGA GTGGTAAGGA TTCTGGGAAC
    AGTCCTGTAA ACTCATCAAT TTCCACTAAT TACCTTGACA TTGGATGCGA CACGCCAACT
    TTTCCCACTT TCCATCGGGC GGATGGAGAG GTTGCACAAG AGACAGTCAT CAATCAAACA
    GGACTGACTC TAGAGATAGA TGCTGGGAAG GAACCAACTG CCATTCAGAG CTCTCAGAGG
    ATTCAGGCAT TGAAGGCAGT GTTTGACAGA GACATTGGAC GAAGCATGCA AACAGGACTG
    AGGACCCCTG TGAGAAGGTC CAAAAGTGAA GGACAAATGA AAAAGGCAGA CGGCCCTTCG
    GTGGTGCCAG AAGTTTGTAC TGACGCAACT TTAAACGACA GGCTCTGGAG CAAACTTGAC
    CCCAGCAGCC ATCGGGATAG TGTGTCATCG TCTTCCAGCA TTTCGTCCAA TGACACTGTG
    ATTGATCTGT CCTTGCCCAA CCTTTCCAGG AAAAGCCTTA CCTGTCTTAG CGCAGCCCGG
    GATTATTCAG ACAAGCAGGC TTCGTTGACC AACAACCATC GGTCGGTCAT TGCAGGATGT
    GGAGTCTCGC TTGTCAGCAA GAGCAAGTCC AACCCCAACC TGCGGGATGG CGAGATGTGC
    AATACAGATG ACGATTTGCA ACCCAGACCT CTGGTAGCCG ATAAAGACCC CATCCGACCC
    ATGCAAAGGC GACACACGTG GAGCAGGTTG TACGTTGAAG GCCTAAAGCA GTCATCGACA
    CTTGCTGCTT CTGCAGCCTC CAAGAAGTCA ACTACCCTTT CAGCAAAAGA CATCGACTCA
    AAGTCCAAAA GTCTGGGAGA TCTCACTTCA GACGATATCA TCTGTAACTT CGAGAGCAAG
    TATCGCAGCA TTAGCCGCAG TTTCATCGTG AGGCCAAGTC GCCAACATGG GAAAACTCTG
    AGGAAACCCC AAAATGATTT CACTGAGCAG CTGAAGAGGC TCACTGACGT GGAACCCCTT
    ACCAGCAAGG ACTTTGCTCA GTGCCGGGCT GACTCAGAAG CAGGACAGGA AGAAGAGGAG
    ATGCCTCCTT TACGGAGGAG TTCATCACGG AGCCAGAGCC GCGTGAGATA CATCGCTAAC
    CGTGCCAGGC AGGCCCAGGA AAGGCAACGG CTACAAAGCA TAACCCGGAT GTCTGGGAGC
    CCCATCGAAG AGCGTGGGAA CCCTGAAGGA GCTTGTAGTG TACCCCATAG TCCTTGCACT
    TGCCTGGATC TTTTAAGCCA GCTTCCACCA GGACCACCCC AACAAAGCCC ACTGAGCCCG
    GACAATGAGG TCTTCTTTAT GCTCAGGCTG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa60126
    Clone ID Related Accession (Same CDS sequence) XM_017357542.1 , XM_017357542.2
    Accession Version XM_017357542.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4473bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017357542.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAGAACA TCGGAGCTCT GCCTCAGAAA CACATGGAAA AATGCATGAG TTCAATGCAG 
    ACGGGCACTC AGATGGTAAA ACTCCGTGGA GGATCCAAAG GCCTGGTGCG CTTTTTCTAC
    TTGGATGAAC ACAAGTCCTG TATCCGCTGG CGACCGTCTA GGAAGAATGA GAAAGCCAAA
    ATCTCGATCG ACTCCATTCG GGAGGTTTGT GAGGGGAAGC AGTCTGAGAT CTTCCAGCGG
    TATTCAGACG GTAGCTTTGA TCCCAACTGC TGCTTCAGCA TCTACTATGG CGAGCACATG
    GAGTCACTTG ACCTGGTGTC TGGCACAGGA GAAGAGGCTC GCACCTGGAT TACTGGCCTT
    AAGTACCTTA TGGCCGGCAT CAGCGATGAA GACAGTCTGG CTAAACGACA ACGCACTCGT
    GACCAGTGGC TGAAGCAGAC GTTTGCAGAG GCTGATAAGA ATGGAGACGG CTGCCTCAGC
    ATCAGTGAAG TCCTCCAGCT CCTGCACAAG CTCAACGTCA ACCTGCCCAG GCAGAAGGTC
    AAGCAGATGT TTAAGGAAGC AGACACTGAT GACAACCAGG GTACTTTGGG ATTTGAGGAG
    TTCTGCTCTT TCTATAAGAT GATGTCAACG CGGCGTGATC TGTACCTGCT GATGCTCACC
    TACAGCAATC ATAAAGACCA CCTGGACACT GATGACCTCA AGCGCTTCCT CGAGACGGAA
    CAAAAGATGA CAAATGTCTC TAAGGAGCAC TGTCTTGAAA TCGTGGCCAA GTTTGAACCT
    TGTCCTGAAA ACCAGACATC GGGTGTGCTG GGGATTGATG GTTTTACAAA CTACATGCGC
    AGCCCTGCGG GGGACATCTT CAACCCTGAA CATTACGAGG TGAACCAAGA CATGACGCAG
    CCCCTCAGTG ATTACTTCAT CGCTTCCTCC CACAACACGT ATCTGATGGG AGACCAGCTC
    ATGTCTCAGT CTCGGGTTGA TATGTACGCC TGGGTGCTGC AGGCCGGATG CAGATGTGTG
    GAAGTGGATT GCTGGGATGG ACAGGATGGA GAACCAATCG TGCATCATGG CTACACCCTG
    ACTTCCAAAA TCCTTTTTAA AGACGTCATT GAAACCATCA ACAAATACGC ATTCGCCAAA
    AACCAGTATC CAGTGATTCT GTCCATCGAG AATCACTGCA GCGTCCCTCA GCAAAAGAAA
    ATGGCTCAGT ATCTGACTGA AGTGTTGGGG GATAAACTGG ACGTGTCCTC CATCCCCGCT
    GACCCGTCAG GACTTCTGCC TTCACCTGAA GCCCTGAAAG GCAAAATACT GATCAAGGGC
    AAGAAACTAC CCTCAAATAT AGATGAGAAC GCAGAGGAGG GGGACGTGTC TGATGAAGAC
    AGTGCAGATG AGATGGAGGA CGATTGTAAG CTGTTGAATG GAGATACGTC TATGAACCGA
    AAGCAGGCGG AGAATGTGGC CAAGAAAAAA TTAGATAACC TCATGAAGGA GTCAAAGATT
    CGCGACCGGG AAGATCCAGA CAGTTTTACC ATCACAGCCC TGCCACCGTC AGGAAAACAT
    GACAAATCGC CACTGAAGGG AAAAGCTGAA GATAGTACCG ACACTGGAGA TGAGGCCAAC
    ATCGGTGGAA ACAAACGTCT GGCCAGAACC TTCATGGGCA GCTTTTCCAA ACGCAAGAAA
    AAGACTAGCA AAGTGAAGAA ATCCTCTAGT TTTGAGGACA CCGACACCGA TCAGGAGAGC
    TCAAGCGGGG CATCTAAAGC ACAAGCACAT CACAGCAGGA AGAAAAAAAC CATGAAGCTG
    TCAAGAGCCC TTTCTGACCT GGTATACATG AAATCTGTGG GAATGCCAGA CTTTGAGTCT
    CAAGGTGTCA CTTGGCAGGT GTCGTCGATG AGCGAGACCA AAGCTCATCA GTTAATCCAG
    CAGAAGCCGG TGCCGTTCAT GCGGTTCAAC CAGCGGCAGC TCTCTCGCGT CTACCCATCT
    CCTTACCGCG TGGACTCCAG CAACTTCAAC CCACAGCCCT TCTGGAACGC TGGCTGCCAT
    CTGGTTGCGC TGAATTACCA GTCAGAGGGA AGAGTGCTGC AGCTCAACAG AGCTAAGTTC
    TATTGCAATG GCAACTGTGG TTACATTCAG AAGCCTGGCT GCTTGTGTGA AGGTTCCTTT
    AATCCAGTAG CTGAGGATCC TTTGCCAGGT CGATTGAAAA AGCAACTGAT TCTTAAGGTC
    ATCAGTGGTC AGCAATTGCC CAAACCAAAA GACTCCATGC TGGGGGACAG AGGAGAGATA
    ATCGATCCGT TTGTGGAAGT GGAGATTATT GGGCTACCGG TGGATTGCTG CAAAGAGCAA
    ACCAGAGTGG TTGACGACAA TGGATTCAAT CCTATGTGGG AAGAAACTCT CGTCTTCACT
    CTGCACATGC CAGAGAACGC TCTTATACGC TTCCTGGTGT GGGATCATGA TCCGATAGGC
    CAAGATTTCA TTGGTCAGAG GACCATCGCC TTTAACAGCA TGATTCCCGG TTACCGCCAT
    GTGTATTTGG AAGGCATGGA GGAAGCTTCT ATCTTTGTTC ATGTTGCAGT GAATGACATC
    ACCAGTAAGG CTCGAGCAGT CAGTGGTATA AAAGGACTCT TTCACAGAAA CCCAAAGCAA
    GCATCTCTCG ACAGCCATGC TGCTGCTCTG CTCAACCGTA AGAGTACCTT CGGTGCTCAC
    CTCCTGCGCC GCACAGCCAG TGCCCCCACC AAGGGCCAAC CCAAAGTCAA GAGGGGCTTC
    CCCGAGATCT CCATTGACAC AAAAGATTGC AGCTCGGACG GCGCCAGCGA GGAGCGGGAA
    TCTGAAGAGG GGCCTCTTGT CCACCACAAT GGAGATACTG CGTCTGCAAA GTCATGGGGT
    CAAGGCACCA ACGGGCAGCT TCACCCTGAT GACAGAAAAG AAAATTCTGA AGTGCAGAGT
    CCAACTCCAT CTCTGAACGC CTCATGTCTG GAATCCATCA CTGAGAACTT TCAGGAGAAC
    AGCCCTTCGA CCTCAACGCA AAGTCCTGTG GATACCTCAC CGCATTGTCC CTTGTCCTCA
    CCATCCACAG CAATAACGGC ATCTTCCAAC GGGGAAAATC TCAGCCCTGT GAAAAATAAC
    GAGGACATCC TGAAATCATT TGCACTAACC CAATCTACGA GTGGTAAGGA TTCTGGGAAC
    AGTCCTGTAA ACTCATCAAT TTCCACTAAT TACCTTGACA TTGGATGCGA CACGCCAACT
    TTTCCCACTT TCCATCGGGC GGATGGAGAG GTTGCACAAG AGACAGTCAT CAATCAAACA
    GGACTGACTC TAGAGATAGA TGCTGGGAAG GAACCAACTG CCATTCAGAG CTCTCAGAGG
    ATTCAGGCAT TGAAGGCAGT GTTTGACAGA GACATTGGAC GAAGCATGCA AACAGGACTG
    AGGACCCCTG TGAGAAGGTC CAAAAGTGAA GGACAAATGA AAAAGGCAGA CGGCCCTTCG
    GTGGTGCCAG AAGTTTGTAC TGACGCAACT TTAAACGACA GGCTCTGGAG CAAACTTGAC
    CCCAGCAGCC ATCGGGATAG TGTGTCATCG TCTTCCAGCA TTTCGTCCAA TGACACTGTG
    ATTGATCTGT CCTTGCCCAA CCTTTCCAGG AAAAGCCTTA CCTGTCTTAG CGCAGCCCGG
    GATTATTCAG ACAAGCAGGC TTCGTTGACC AACAACCATC GGTCGGTCAT TGCAGGATGT
    GGAGTCTCGC TTGTCAGCAA GAGCAAGTCC AACCCCAACC TGCGGGATGG CGAGATGTGC
    AATACAGATG ACGATTTGCA ACCCAGACCT CTGGTAGCCG ATAAAGACCC CATCCGACCC
    ATGCAAAGGC GACACACGTG GAGCAGGTTG TACGTTGAAG GCCTAAAGCA GTCATCGACA
    CTTGCTGCTT CTGCAGCCTC CAAGAAGTCA ACTACCCTTT CAGCAAAAGA CATCGACTCA
    AAGTCCAAAA GTCTGGGAGA TCTCACTTCA GACGATATCA TCTGTAACTT CGAGAGCAAG
    TATCGCAGCA TTAGCCGCAG TTTCATCGTG AGGCCAAGTC GCCAACATGG GAAAACTCTG
    AGGAAACCCC AAAATGATTT CACTGAGCAG CTGAAGAGGC TCACTGACGT GGAACCCCTT
    ACCAGCAAGG ACTTTGCTCA GTGCCGGGCT GACTCAGAAG CAGGACAGGA AGAAGAGGAG
    ATGCCTCCTT TACGGAGGAG TTCATCACGG AGCCAGAGCC GCGTGAGATA CATCGCTAAC
    CGTGCCAGGC AGGCCCAGGA AAGGCAACGG CTACAAAGCA TAACCCGGAT GTCTGGGAGC
    CCCATCGAAG AGCGTGGGAA CCCTGAAGGA GCTTGTAGTG TACCCCATAG TCCTTGCACT
    TGCCTGGATC TTTTAAGCCA GCTTCCACCA GGACCACCCC AACAAAGCCC ACTGAGCCCG
    GACAATGAGG TCTTCTTTAT GCTCAGGCTG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017213031.1
    CDS26..4498
    Translation

    Target ORF information:

    RefSeq Version XM_017357542.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio phospholipase C, eta 2a (plch2a), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017357542.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAGAACA TCGGAGCTCT GCCTCAGAAA CACATGGAAA AATGCATGAG TTCAATGCAG 
    ACGGGCACTC AGATGGTAAA ACTCCGTGGA GGATCCAAAG GCCTGGTGCG CTTTTTCTAC
    TTGGATGAAC ACAAGTCCTG TATCCGCTGG CGACCGTCTA GGAAGAATGA GAAAGCCAAA
    ATCTCGATCG ACTCCATTCG GGAGGTTTGT GAGGGGAAGC AGTCTGAGAT CTTCCAGCGG
    TATTCAGACG GTAGCTTTGA TCCCAACTGC TGCTTCAGCA TCTACTATGG CGAGCACATG
    GAGTCACTTG ACCTGGTGTC TGGCACAGGA GAAGAGGCTC GCACCTGGAT TACTGGCCTT
    AAGTACCTTA TGGCCGGCAT CAGCGATGAA GACAGTCTGG CTAAACGACA ACGCACTCGT
    GACCAGTGGC TGAAGCAGAC GTTTGCAGAG GCTGATAAGA ATGGAGACGG CTGCCTCAGC
    ATCAGTGAAG TCCTCCAGCT CCTGCACAAG CTCAACGTCA ACCTGCCCAG GCAGAAGGTC
    AAGCAGATGT TTAAGGAAGC AGACACTGAT GACAACCAGG GTACTTTGGG ATTTGAGGAG
    TTCTGCTCTT TCTATAAGAT GATGTCAACG CGGCGTGATC TGTACCTGCT GATGCTCACC
    TACAGCAATC ATAAAGACCA CCTGGACACT GATGACCTCA AGCGCTTCCT CGAGACGGAA
    CAAAAGATGA CAAATGTCTC TAAGGAGCAC TGTCTTGAAA TCGTGGCCAA GTTTGAACCT
    TGTCCTGAAA ACCAGACATC GGGTGTGCTG GGGATTGATG GTTTTACAAA CTACATGCGC
    AGCCCTGCGG GGGACATCTT CAACCCTGAA CATTACGAGG TGAACCAAGA CATGACGCAG
    CCCCTCAGTG ATTACTTCAT CGCTTCCTCC CACAACACGT ATCTGATGGG AGACCAGCTC
    ATGTCTCAGT CTCGGGTTGA TATGTACGCC TGGGTGCTGC AGGCCGGATG CAGATGTGTG
    GAAGTGGATT GCTGGGATGG ACAGGATGGA GAACCAATCG TGCATCATGG CTACACCCTG
    ACTTCCAAAA TCCTTTTTAA AGACGTCATT GAAACCATCA ACAAATACGC ATTCGCCAAA
    AACCAGTATC CAGTGATTCT GTCCATCGAG AATCACTGCA GCGTCCCTCA GCAAAAGAAA
    ATGGCTCAGT ATCTGACTGA AGTGTTGGGG GATAAACTGG ACGTGTCCTC CATCCCCGCT
    GACCCGTCAG GACTTCTGCC TTCACCTGAA GCCCTGAAAG GCAAAATACT GATCAAGGGC
    AAGAAACTAC CCTCAAATAT AGATGAGAAC GCAGAGGAGG GGGACGTGTC TGATGAAGAC
    AGTGCAGATG AGATGGAGGA CGATTGTAAG CTGTTGAATG GAGATACGTC TATGAACCGA
    AAGCAGGCGG AGAATGTGGC CAAGAAAAAA TTAGATAACC TCATGAAGGA GTCAAAGATT
    CGCGACCGGG AAGATCCAGA CAGTTTTACC ATCACAGCCC TGCCACCGTC AGGAAAACAT
    GACAAATCGC CACTGAAGGG AAAAGCTGAA GATAGTACCG ACACTGGAGA TGAGGCCAAC
    ATCGGTGGAA ACAAACGTCT GGCCAGAACC TTCATGGGCA GCTTTTCCAA ACGCAAGAAA
    AAGACTAGCA AAGTGAAGAA ATCCTCTAGT TTTGAGGACA CCGACACCGA TCAGGAGAGC
    TCAAGCGGGG CATCTAAAGC ACAAGCACAT CACAGCAGGA AGAAAAAAAC CATGAAGCTG
    TCAAGAGCCC TTTCTGACCT GGTATACATG AAATCTGTGG GAATGCCAGA CTTTGAGTCT
    CAAGGTGTCA CTTGGCAGGT GTCGTCGATG AGCGAGACCA AAGCTCATCA GTTAATCCAG
    CAGAAGCCGG TGCCGTTCAT GCGGTTCAAC CAGCGGCAGC TCTCTCGCGT CTACCCATCT
    CCTTACCGCG TGGACTCCAG CAACTTCAAC CCACAGCCCT TCTGGAACGC TGGCTGCCAT
    CTGGTTGCGC TGAATTACCA GTCAGAGGGA AGAGTGCTGC AGCTCAACAG AGCTAAGTTC
    TATTGCAATG GCAACTGTGG TTACATTCAG AAGCCTGGCT GCTTGTGTGA AGGTTCCTTT
    AATCCAGTAG CTGAGGATCC TTTGCCAGGT CGATTGAAAA AGCAACTGAT TCTTAAGGTC
    ATCAGTGGTC AGCAATTGCC CAAACCAAAA GACTCCATGC TGGGGGACAG AGGAGAGATA
    ATCGATCCGT TTGTGGAAGT GGAGATTATT GGGCTACCGG TGGATTGCTG CAAAGAGCAA
    ACCAGAGTGG TTGACGACAA TGGATTCAAT CCTATGTGGG AAGAAACTCT CGTCTTCACT
    CTGCACATGC CAGAGAACGC TCTTATACGC TTCCTGGTGT GGGATCATGA TCCGATAGGC
    CAAGATTTCA TTGGTCAGAG GACCATCGCC TTTAACAGCA TGATTCCCGG TTACCGCCAT
    GTGTATTTGG AAGGCATGGA GGAAGCTTCT ATCTTTGTTC ATGTTGCAGT GAATGACATC
    ACCAGTAAGG CTCGAGCAGT CAGTGGTATA AAAGGACTCT TTCACAGAAA CCCAAAGCAA
    GCATCTCTCG ACAGCCATGC TGCTGCTCTG CTCAACCGTA AGAGTACCTT CGGTGCTCAC
    CTCCTGCGCC GCACAGCCAG TGCCCCCACC AAGGGCCAAC CCAAAGTCAA GAGGGGCTTC
    CCCGAGATCT CCATTGACAC AAAAGATTGC AGCTCGGACG GCGCCAGCGA GGAGCGGGAA
    TCTGAAGAGG GGCCTCTTGT CCACCACAAT GGAGATACTG CGTCTGCAAA GTCATGGGGT
    CAAGGCACCA ACGGGCAGCT TCACCCTGAT GACAGAAAAG AAAATTCTGA AGTGCAGAGT
    CCAACTCCAT CTCTGAACGC CTCATGTCTG GAATCCATCA CTGAGAACTT TCAGGAGAAC
    AGCCCTTCGA CCTCAACGCA AAGTCCTGTG GATACCTCAC CGCATTGTCC CTTGTCCTCA
    CCATCCACAG CAATAACGGC ATCTTCCAAC GGGGAAAATC TCAGCCCTGT GAAAAATAAC
    GAGGACATCC TGAAATCATT TGCACTAACC CAATCTACGA GTGGTAAGGA TTCTGGGAAC
    AGTCCTGTAA ACTCATCAAT TTCCACTAAT TACCTTGACA TTGGATGCGA CACGCCAACT
    TTTCCCACTT TCCATCGGGC GGATGGAGAG GTTGCACAAG AGACAGTCAT CAATCAAACA
    GGACTGACTC TAGAGATAGA TGCTGGGAAG GAACCAACTG CCATTCAGAG CTCTCAGAGG
    ATTCAGGCAT TGAAGGCAGT GTTTGACAGA GACATTGGAC GAAGCATGCA AACAGGACTG
    AGGACCCCTG TGAGAAGGTC CAAAAGTGAA GGACAAATGA AAAAGGCAGA CGGCCCTTCG
    GTGGTGCCAG AAGTTTGTAC TGACGCAACT TTAAACGACA GGCTCTGGAG CAAACTTGAC
    CCCAGCAGCC ATCGGGATAG TGTGTCATCG TCTTCCAGCA TTTCGTCCAA TGACACTGTG
    ATTGATCTGT CCTTGCCCAA CCTTTCCAGG AAAAGCCTTA CCTGTCTTAG CGCAGCCCGG
    GATTATTCAG ACAAGCAGGC TTCGTTGACC AACAACCATC GGTCGGTCAT TGCAGGATGT
    GGAGTCTCGC TTGTCAGCAA GAGCAAGTCC AACCCCAACC TGCGGGATGG CGAGATGTGC
    AATACAGATG ACGATTTGCA ACCCAGACCT CTGGTAGCCG ATAAAGACCC CATCCGACCC
    ATGCAAAGGC GACACACGTG GAGCAGGTTG TACGTTGAAG GCCTAAAGCA GTCATCGACA
    CTTGCTGCTT CTGCAGCCTC CAAGAAGTCA ACTACCCTTT CAGCAAAAGA CATCGACTCA
    AAGTCCAAAA GTCTGGGAGA TCTCACTTCA GACGATATCA TCTGTAACTT CGAGAGCAAG
    TATCGCAGCA TTAGCCGCAG TTTCATCGTG AGGCCAAGTC GCCAACATGG GAAAACTCTG
    AGGAAACCCC AAAATGATTT CACTGAGCAG CTGAAGAGGC TCACTGACGT GGAACCCCTT
    ACCAGCAAGG ACTTTGCTCA GTGCCGGGCT GACTCAGAAG CAGGACAGGA AGAAGAGGAG
    ATGCCTCCTT TACGGAGGAG TTCATCACGG AGCCAGAGCC GCGTGAGATA CATCGCTAAC
    CGTGCCAGGC AGGCCCAGGA AAGGCAACGG CTACAAAGCA TAACCCGGAT GTCTGGGAGC
    CCCATCGAAG AGCGTGGGAA CCCTGAAGGA GCTTGTAGTG TACCCCATAG TCCTTGCACT
    TGCCTGGATC TTTTAAGCCA GCTTCCACCA GGACCACCCC AACAAAGCCC ACTGAGCCCG
    GACAATGAGG TCTTCTTTAT GCTCAGGCTG TAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa49932
    Clone ID Related Accession (Same CDS sequence) XM_009304268.2 , XM_009304268.3
    Accession Version XM_009304268.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3132bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X5
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304268.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTGTGCCG CTGTCTTGCC TGCCAGCTCG GTCACTTCTG TCGTGATGCC CCCAGCATTA 
    GGGCCAGTAC ATGCTACTAA ATCAGCAACT CCACCGGTCC GAGTACCGAG CCCAGTGGTG
    GCATCAGCCT GCTCGAAGGC ACTGCGTAAG CTGACCCTGT CAAGACTCCT TGCGGGGTTC
    TTTTGGACTG GAGAGAATAC TGTGTATGGA TCCAAATGGA GGCTTGGCTT GTTTGTGGAA
    AAATGCATGA GTTCAATGCA GACGGGCACT CAGATGGTAA AACTCCGTGG AGGATCCAAA
    GGCCTGGTGC GCTTTTTCTA CTTGGATGAA CACAAGTCCT GTATCCGCTG GCGACCGTCT
    AGGAAGAATG AGAAAGCCAA AATCTCGATC GACTCCATTC GGGAGGTTTG TGAGGGGAAG
    CAGTCTGAGA TCTTCCAGCG GTATTCAGAC GGTAGCTTTG ATCCCAACTG CTGCTTCAGC
    ATCTACTATG GCGAGCACAT GGAGTCACTT GACCTGGTGT CTGGCACAGG AGAAGAGGCT
    CGCACCTGGA TTACTGGCCT TAAGTACCTT ATGGCCGGCA TCAGCGATGA AGACAGTCTG
    GCTAAACGAC AACGCACTCG TGACCAGTGG CTGAAGCAGA CGTTTGCAGA GGCTGATAAG
    AATGGAGACG GCTGCCTCAG CATCAGTGAA GTCCTCCAGC TCCTGCACAA GCTCAACGTC
    AACCTGCCCA GGCAGAAGGT CAAGCAGATG TTTAAGGAAG CAGACACTGA TGACAACCAG
    GGTACTTTGG GATTTGAGGA GTTCTGCTCT TTCTATAAGA TGATGTCAAC GCGGCGTGAT
    CTGTACCTGC TGATGCTCAC CTACAGCAAT CATAAAGACC ACCTGGACAC TGATGACCTC
    AAGCGCTTCC TCGAGACGGA ACAAAAGATG ACAAATGTCT CTAAGGAGCA CTGTCTTGAA
    ATCGTGGCCA AGTTTGAACC TTGTCCTGAA AACCAGACAT CGGGTGTGCT GGGGATTGAT
    GGTTTTACAA ACTACATGCG CAGCCCTGCG GGGGACATCT TCAACCCTGA ACATTACGAG
    GTGAACCAAG ACATGACGCA GCCCCTCAGT GATTACTTCA TCGCTTCCTC CCACAACACG
    TATCTGATGG GAGACCAGCT CATGTCTCAG TCTCGGGTTG ATATGTACGC CTGGGTGCTG
    CAGGCCGGAT GCAGATGTGT GGAAGTGGAT TGCTGGGATG GACAGGATGG AGAACCAATC
    GTGCATCATG GCTACACCCT GACTTCCAAA ATCCTTTTTA AAGACGTCAT TGAAACCATC
    AACAAATACG CATTCGCCAA AAACCAGTAT CCAGTGATTC TGTCCATCGA GAATCACTGC
    AGCGTCCCTC AGCAAAAGAA AATGGCTCAG TATCTGACTG AAGTGTTGGG GGATAAACTG
    GACGTGTCCT CCATCCCCGC TGACCCGTCA GGACTTCTGC CTTCACCTGA AGCCCTGAAA
    GGCAAAATAC TGATCAAGGG CAAGAAACTA CCCTCAAATA TAGATGAGAA CGCAGAGGAG
    GGGGACGTGT CTGATGAAGA CAGTGCAGAT GAGATGGAGG ACGATTGTAA GCTGTTGAAT
    GGAGATACGT CTATGAACCG AAAGCAGGCG GAGAATGTGG CCAAGAAAAA ATTAGATAAC
    CTCATGAAGG AGTCAAAGAT TCGCGACCGG GAAGATCCAG ACAGTTTTAC CATCACAGCC
    CTGCCACCGT CAGGAAAACA TGACAAATCG CCACTGAAGG GAAAAGCTGA AGATAGTACC
    GACACTGGAG ATGAGGCCAA CATCGGTGGA AACAAACGTC TGGCCAGAAC CTTCATGGGC
    AGCTTTTCCA AACGCAAGAA AAAGACTAGC AAAGTGAAGA AATCCTCTAG TTTTGAGGAC
    ACCGACACCG ATCAGGAGAG CTCAAGCGGG GCATCTAAAG CACAAGCACA TCACAGCAGG
    AAGAAAAAAA CCATGAAGCT GTCAAGAGCC CTTTCTGACC TGGTATACAT GAAATCTGTG
    GGAATGCCAG ACTTTGAGTC TCAAGGTGTC ACTTGGCAGG TGTCGTCGAT GAGCGAGACC
    AAAGCTCATC AGTTAATCCA GCAGAAGCCG GTGCCGTTCA TGCGGTTCAA CCAGCGGCAG
    CTCTCTCGCG TCTACCCATC TCCTTACCGC GTGGACTCCA GCAACTTCAA CCCACAGCCC
    TTCTGGAACG CTGGCTGCCA TCTGGTTGCG CTGAATTACC AGTCAGAGGG AAGAGTGCTG
    CAGCTCAACA GAGCTAAGTT CTATTGCAAT GGCAACTGTG GTTACATTCA GAAGCCTGGC
    TGCTTGTGTG AAGGTTCCTT TAATCCAGTA GCTGAGGATC CTTTGCCAGG TCGATTGAAA
    AAGCAACTGA TTCTTAAGGT CATCAGTGGT CAGCAATTGC CCAAACCAAA AGACTCCATG
    CTGGGGGACA GAGGAGAGAT AATCGATCCG TTTGTGGAAG TGGAGATTAT TGGGCTACCG
    GTGGATTGCT GCAAAGAGCA AACCAGAGTG GTTGACGACA ATGGATTCAA TCCTATGTGG
    GAAGAAACTC TCGTCTTCAC TCTGCACATG CCAGAGAACG CTCTTATACG CTTCCTGGTG
    TGGGATCATG ATCCGATAGG CCAAGATTTC ATTGGTCAGA GGACCATCGC CTTTAACAGC
    ATGATTCCCG GTTACCGCCA TGTGTATTTG GAAGGCATGG AGGAAGCTTC TATCTTTGTT
    CATGTTGCAG TGAATGACAT CACCAGTAAG GCTCGAGCAG TCAGTGGTAT AAAAGGACTC
    TTTCACAGAA ACCCAAAGCA AGCATCTCTC GACAGCCATG CTGCTGCTCT GCTCAACCGT
    AAGAGTACCT TCGGTGCTCA CCTCCTGCGC CGCACAGCCA GTGCCCCCAC CAAGGGCCAA
    CCCAAAGTCA AGAGGGGCTT CCCCGAGATC TCCATTGACA CAAAAGATTG CAGCTCGGAC
    GGCGCCAGCG AGGAGCGGGA ATCTGAAGAG GGGCCTCTTG TCCACCACAA TGGAGATACT
    GCGTCTGCAA AGTCATGGGG TCAAGGCACC AACGGGCAGC TTCACCCTGA TGACAGAAAA
    GTGCAAAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009302543.1
    CDS265..3396
    Translation

    Target ORF information:

    RefSeq Version XM_009304268.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio phospholipase C eta 2 (plch2), transcript variant X6, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009304268.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTGTGCCG CTGTCTTGCC TGCCAGCTCG GTCACTTCTG TCGTGATGCC CCCAGCATTA 
    GGGCCAGTAC ATGCTACTAA ATCAGCAACT CCACCGGTCC GAGTACCGAG CCCAGTGGTG
    GCATCAGCCT GCTCGAAGGC ACTGCGTAAG CTGACCCTGT CAAGACTCCT TGCGGGGTTC
    TTTTGGACTG GAGAGAATAC TGTGTATGGA TCCAAATGGA GGCTTGGCTT GTTTGTGGAA
    AAATGCATGA GTTCAATGCA GACGGGCACT CAGATGGTAA AACTCCGTGG AGGATCCAAA
    GGCCTGGTGC GCTTTTTCTA CTTGGATGAA CACAAGTCCT GTATCCGCTG GCGACCGTCT
    AGGAAGAATG AGAAAGCCAA AATCTCGATC GACTCCATTC GGGAGGTTTG TGAGGGGAAG
    CAGTCTGAGA TCTTCCAGCG GTATTCAGAC GGTAGCTTTG ATCCCAACTG CTGCTTCAGC
    ATCTACTATG GCGAGCACAT GGAGTCACTT GACCTGGTGT CTGGCACAGG AGAAGAGGCT
    CGCACCTGGA TTACTGGCCT TAAGTACCTT ATGGCCGGCA TCAGCGATGA AGACAGTCTG
    GCTAAACGAC AACGCACTCG TGACCAGTGG CTGAAGCAGA CGTTTGCAGA GGCTGATAAG
    AATGGAGACG GCTGCCTCAG CATCAGTGAA GTCCTCCAGC TCCTGCACAA GCTCAACGTC
    AACCTGCCCA GGCAGAAGGT CAAGCAGATG TTTAAGGAAG CAGACACTGA TGACAACCAG
    GGTACTTTGG GATTTGAGGA GTTCTGCTCT TTCTATAAGA TGATGTCAAC GCGGCGTGAT
    CTGTACCTGC TGATGCTCAC CTACAGCAAT CATAAAGACC ACCTGGACAC TGATGACCTC
    AAGCGCTTCC TCGAGACGGA ACAAAAGATG ACAAATGTCT CTAAGGAGCA CTGTCTTGAA
    ATCGTGGCCA AGTTTGAACC TTGTCCTGAA AACCAGACAT CGGGTGTGCT GGGGATTGAT
    GGTTTTACAA ACTACATGCG CAGCCCTGCG GGGGACATCT TCAACCCTGA ACATTACGAG
    GTGAACCAAG ACATGACGCA GCCCCTCAGT GATTACTTCA TCGCTTCCTC CCACAACACG
    TATCTGATGG GAGACCAGCT CATGTCTCAG TCTCGGGTTG ATATGTACGC CTGGGTGCTG
    CAGGCCGGAT GCAGATGTGT GGAAGTGGAT TGCTGGGATG GACAGGATGG AGAACCAATC
    GTGCATCATG GCTACACCCT GACTTCCAAA ATCCTTTTTA AAGACGTCAT TGAAACCATC
    AACAAATACG CATTCGCCAA AAACCAGTAT CCAGTGATTC TGTCCATCGA GAATCACTGC
    AGCGTCCCTC AGCAAAAGAA AATGGCTCAG TATCTGACTG AAGTGTTGGG GGATAAACTG
    GACGTGTCCT CCATCCCCGC TGACCCGTCA GGACTTCTGC CTTCACCTGA AGCCCTGAAA
    GGCAAAATAC TGATCAAGGG CAAGAAACTA CCCTCAAATA TAGATGAGAA CGCAGAGGAG
    GGGGACGTGT CTGATGAAGA CAGTGCAGAT GAGATGGAGG ACGATTGTAA GCTGTTGAAT
    GGAGATACGT CTATGAACCG AAAGCAGGCG GAGAATGTGG CCAAGAAAAA ATTAGATAAC
    CTCATGAAGG AGTCAAAGAT TCGCGACCGG GAAGATCCAG ACAGTTTTAC CATCACAGCC
    CTGCCACCGT CAGGAAAACA TGACAAATCG CCACTGAAGG GAAAAGCTGA AGATAGTACC
    GACACTGGAG ATGAGGCCAA CATCGGTGGA AACAAACGTC TGGCCAGAAC CTTCATGGGC
    AGCTTTTCCA AACGCAAGAA AAAGACTAGC AAAGTGAAGA AATCCTCTAG TTTTGAGGAC
    ACCGACACCG ATCAGGAGAG CTCAAGCGGG GCATCTAAAG CACAAGCACA TCACAGCAGG
    AAGAAAAAAA CCATGAAGCT GTCAAGAGCC CTTTCTGACC TGGTATACAT GAAATCTGTG
    GGAATGCCAG ACTTTGAGTC TCAAGGTGTC ACTTGGCAGG TGTCGTCGAT GAGCGAGACC
    AAAGCTCATC AGTTAATCCA GCAGAAGCCG GTGCCGTTCA TGCGGTTCAA CCAGCGGCAG
    CTCTCTCGCG TCTACCCATC TCCTTACCGC GTGGACTCCA GCAACTTCAA CCCACAGCCC
    TTCTGGAACG CTGGCTGCCA TCTGGTTGCG CTGAATTACC AGTCAGAGGG AAGAGTGCTG
    CAGCTCAACA GAGCTAAGTT CTATTGCAAT GGCAACTGTG GTTACATTCA GAAGCCTGGC
    TGCTTGTGTG AAGGTTCCTT TAATCCAGTA GCTGAGGATC CTTTGCCAGG TCGATTGAAA
    AAGCAACTGA TTCTTAAGGT CATCAGTGGT CAGCAATTGC CCAAACCAAA AGACTCCATG
    CTGGGGGACA GAGGAGAGAT AATCGATCCG TTTGTGGAAG TGGAGATTAT TGGGCTACCG
    GTGGATTGCT GCAAAGAGCA AACCAGAGTG GTTGACGACA ATGGATTCAA TCCTATGTGG
    GAAGAAACTC TCGTCTTCAC TCTGCACATG CCAGAGAACG CTCTTATACG CTTCCTGGTG
    TGGGATCATG ATCCGATAGG CCAAGATTTC ATTGGTCAGA GGACCATCGC CTTTAACAGC
    ATGATTCCCG GTTACCGCCA TGTGTATTTG GAAGGCATGG AGGAAGCTTC TATCTTTGTT
    CATGTTGCAG TGAATGACAT CACCAGTAAG GCTCGAGCAG TCAGTGGTAT AAAAGGACTC
    TTTCACAGAA ACCCAAAGCA AGCATCTCTC GACAGCCATG CTGCTGCTCT GCTCAACCGT
    AAGAGTACCT TCGGTGCTCA CCTCCTGCGC CGCACAGCCA GTGCCCCCAC CAAGGGCCAA
    CCCAAAGTCA AGAGGGGCTT CCCCGAGATC TCCATTGACA CAAAAGATTG CAGCTCGGAC
    GGCGCCAGCG AGGAGCGGGA ATCTGAAGAG GGGCCTCTTG TCCACCACAA TGGAGATACT
    GCGTCTGCAA AGTCATGGGG TCAAGGCACC AACGGGCAGC TTCACCCTGA TGACAGAAAA
    GTGCAAAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa49932
    Clone ID Related Accession (Same CDS sequence) XM_009304268.2 , XM_009304268.3
    Accession Version XM_009304268.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3132bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304268.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTGTGCCG CTGTCTTGCC TGCCAGCTCG GTCACTTCTG TCGTGATGCC CCCAGCATTA 
    GGGCCAGTAC ATGCTACTAA ATCAGCAACT CCACCGGTCC GAGTACCGAG CCCAGTGGTG
    GCATCAGCCT GCTCGAAGGC ACTGCGTAAG CTGACCCTGT CAAGACTCCT TGCGGGGTTC
    TTTTGGACTG GAGAGAATAC TGTGTATGGA TCCAAATGGA GGCTTGGCTT GTTTGTGGAA
    AAATGCATGA GTTCAATGCA GACGGGCACT CAGATGGTAA AACTCCGTGG AGGATCCAAA
    GGCCTGGTGC GCTTTTTCTA CTTGGATGAA CACAAGTCCT GTATCCGCTG GCGACCGTCT
    AGGAAGAATG AGAAAGCCAA AATCTCGATC GACTCCATTC GGGAGGTTTG TGAGGGGAAG
    CAGTCTGAGA TCTTCCAGCG GTATTCAGAC GGTAGCTTTG ATCCCAACTG CTGCTTCAGC
    ATCTACTATG GCGAGCACAT GGAGTCACTT GACCTGGTGT CTGGCACAGG AGAAGAGGCT
    CGCACCTGGA TTACTGGCCT TAAGTACCTT ATGGCCGGCA TCAGCGATGA AGACAGTCTG
    GCTAAACGAC AACGCACTCG TGACCAGTGG CTGAAGCAGA CGTTTGCAGA GGCTGATAAG
    AATGGAGACG GCTGCCTCAG CATCAGTGAA GTCCTCCAGC TCCTGCACAA GCTCAACGTC
    AACCTGCCCA GGCAGAAGGT CAAGCAGATG TTTAAGGAAG CAGACACTGA TGACAACCAG
    GGTACTTTGG GATTTGAGGA GTTCTGCTCT TTCTATAAGA TGATGTCAAC GCGGCGTGAT
    CTGTACCTGC TGATGCTCAC CTACAGCAAT CATAAAGACC ACCTGGACAC TGATGACCTC
    AAGCGCTTCC TCGAGACGGA ACAAAAGATG ACAAATGTCT CTAAGGAGCA CTGTCTTGAA
    ATCGTGGCCA AGTTTGAACC TTGTCCTGAA AACCAGACAT CGGGTGTGCT GGGGATTGAT
    GGTTTTACAA ACTACATGCG CAGCCCTGCG GGGGACATCT TCAACCCTGA ACATTACGAG
    GTGAACCAAG ACATGACGCA GCCCCTCAGT GATTACTTCA TCGCTTCCTC CCACAACACG
    TATCTGATGG GAGACCAGCT CATGTCTCAG TCTCGGGTTG ATATGTACGC CTGGGTGCTG
    CAGGCCGGAT GCAGATGTGT GGAAGTGGAT TGCTGGGATG GACAGGATGG AGAACCAATC
    GTGCATCATG GCTACACCCT GACTTCCAAA ATCCTTTTTA AAGACGTCAT TGAAACCATC
    AACAAATACG CATTCGCCAA AAACCAGTAT CCAGTGATTC TGTCCATCGA GAATCACTGC
    AGCGTCCCTC AGCAAAAGAA AATGGCTCAG TATCTGACTG AAGTGTTGGG GGATAAACTG
    GACGTGTCCT CCATCCCCGC TGACCCGTCA GGACTTCTGC CTTCACCTGA AGCCCTGAAA
    GGCAAAATAC TGATCAAGGG CAAGAAACTA CCCTCAAATA TAGATGAGAA CGCAGAGGAG
    GGGGACGTGT CTGATGAAGA CAGTGCAGAT GAGATGGAGG ACGATTGTAA GCTGTTGAAT
    GGAGATACGT CTATGAACCG AAAGCAGGCG GAGAATGTGG CCAAGAAAAA ATTAGATAAC
    CTCATGAAGG AGTCAAAGAT TCGCGACCGG GAAGATCCAG ACAGTTTTAC CATCACAGCC
    CTGCCACCGT CAGGAAAACA TGACAAATCG CCACTGAAGG GAAAAGCTGA AGATAGTACC
    GACACTGGAG ATGAGGCCAA CATCGGTGGA AACAAACGTC TGGCCAGAAC CTTCATGGGC
    AGCTTTTCCA AACGCAAGAA AAAGACTAGC AAAGTGAAGA AATCCTCTAG TTTTGAGGAC
    ACCGACACCG ATCAGGAGAG CTCAAGCGGG GCATCTAAAG CACAAGCACA TCACAGCAGG
    AAGAAAAAAA CCATGAAGCT GTCAAGAGCC CTTTCTGACC TGGTATACAT GAAATCTGTG
    GGAATGCCAG ACTTTGAGTC TCAAGGTGTC ACTTGGCAGG TGTCGTCGAT GAGCGAGACC
    AAAGCTCATC AGTTAATCCA GCAGAAGCCG GTGCCGTTCA TGCGGTTCAA CCAGCGGCAG
    CTCTCTCGCG TCTACCCATC TCCTTACCGC GTGGACTCCA GCAACTTCAA CCCACAGCCC
    TTCTGGAACG CTGGCTGCCA TCTGGTTGCG CTGAATTACC AGTCAGAGGG AAGAGTGCTG
    CAGCTCAACA GAGCTAAGTT CTATTGCAAT GGCAACTGTG GTTACATTCA GAAGCCTGGC
    TGCTTGTGTG AAGGTTCCTT TAATCCAGTA GCTGAGGATC CTTTGCCAGG TCGATTGAAA
    AAGCAACTGA TTCTTAAGGT CATCAGTGGT CAGCAATTGC CCAAACCAAA AGACTCCATG
    CTGGGGGACA GAGGAGAGAT AATCGATCCG TTTGTGGAAG TGGAGATTAT TGGGCTACCG
    GTGGATTGCT GCAAAGAGCA AACCAGAGTG GTTGACGACA ATGGATTCAA TCCTATGTGG
    GAAGAAACTC TCGTCTTCAC TCTGCACATG CCAGAGAACG CTCTTATACG CTTCCTGGTG
    TGGGATCATG ATCCGATAGG CCAAGATTTC ATTGGTCAGA GGACCATCGC CTTTAACAGC
    ATGATTCCCG GTTACCGCCA TGTGTATTTG GAAGGCATGG AGGAAGCTTC TATCTTTGTT
    CATGTTGCAG TGAATGACAT CACCAGTAAG GCTCGAGCAG TCAGTGGTAT AAAAGGACTC
    TTTCACAGAA ACCCAAAGCA AGCATCTCTC GACAGCCATG CTGCTGCTCT GCTCAACCGT
    AAGAGTACCT TCGGTGCTCA CCTCCTGCGC CGCACAGCCA GTGCCCCCAC CAAGGGCCAA
    CCCAAAGTCA AGAGGGGCTT CCCCGAGATC TCCATTGACA CAAAAGATTG CAGCTCGGAC
    GGCGCCAGCG AGGAGCGGGA ATCTGAAGAG GGGCCTCTTG TCCACCACAA TGGAGATACT
    GCGTCTGCAA AGTCATGGGG TCAAGGCACC AACGGGCAGC TTCACCCTGA TGACAGAAAA
    GTGCAAAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009302543.1
    CDS271..3402
    Translation

    Target ORF information:

    RefSeq Version XM_009304268.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio phospholipase C, eta 2a (plch2a), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009304268.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTGTGCCG CTGTCTTGCC TGCCAGCTCG GTCACTTCTG TCGTGATGCC CCCAGCATTA 
    GGGCCAGTAC ATGCTACTAA ATCAGCAACT CCACCGGTCC GAGTACCGAG CCCAGTGGTG
    GCATCAGCCT GCTCGAAGGC ACTGCGTAAG CTGACCCTGT CAAGACTCCT TGCGGGGTTC
    TTTTGGACTG GAGAGAATAC TGTGTATGGA TCCAAATGGA GGCTTGGCTT GTTTGTGGAA
    AAATGCATGA GTTCAATGCA GACGGGCACT CAGATGGTAA AACTCCGTGG AGGATCCAAA
    GGCCTGGTGC GCTTTTTCTA CTTGGATGAA CACAAGTCCT GTATCCGCTG GCGACCGTCT
    AGGAAGAATG AGAAAGCCAA AATCTCGATC GACTCCATTC GGGAGGTTTG TGAGGGGAAG
    CAGTCTGAGA TCTTCCAGCG GTATTCAGAC GGTAGCTTTG ATCCCAACTG CTGCTTCAGC
    ATCTACTATG GCGAGCACAT GGAGTCACTT GACCTGGTGT CTGGCACAGG AGAAGAGGCT
    CGCACCTGGA TTACTGGCCT TAAGTACCTT ATGGCCGGCA TCAGCGATGA AGACAGTCTG
    GCTAAACGAC AACGCACTCG TGACCAGTGG CTGAAGCAGA CGTTTGCAGA GGCTGATAAG
    AATGGAGACG GCTGCCTCAG CATCAGTGAA GTCCTCCAGC TCCTGCACAA GCTCAACGTC
    AACCTGCCCA GGCAGAAGGT CAAGCAGATG TTTAAGGAAG CAGACACTGA TGACAACCAG
    GGTACTTTGG GATTTGAGGA GTTCTGCTCT TTCTATAAGA TGATGTCAAC GCGGCGTGAT
    CTGTACCTGC TGATGCTCAC CTACAGCAAT CATAAAGACC ACCTGGACAC TGATGACCTC
    AAGCGCTTCC TCGAGACGGA ACAAAAGATG ACAAATGTCT CTAAGGAGCA CTGTCTTGAA
    ATCGTGGCCA AGTTTGAACC TTGTCCTGAA AACCAGACAT CGGGTGTGCT GGGGATTGAT
    GGTTTTACAA ACTACATGCG CAGCCCTGCG GGGGACATCT TCAACCCTGA ACATTACGAG
    GTGAACCAAG ACATGACGCA GCCCCTCAGT GATTACTTCA TCGCTTCCTC CCACAACACG
    TATCTGATGG GAGACCAGCT CATGTCTCAG TCTCGGGTTG ATATGTACGC CTGGGTGCTG
    CAGGCCGGAT GCAGATGTGT GGAAGTGGAT TGCTGGGATG GACAGGATGG AGAACCAATC
    GTGCATCATG GCTACACCCT GACTTCCAAA ATCCTTTTTA AAGACGTCAT TGAAACCATC
    AACAAATACG CATTCGCCAA AAACCAGTAT CCAGTGATTC TGTCCATCGA GAATCACTGC
    AGCGTCCCTC AGCAAAAGAA AATGGCTCAG TATCTGACTG AAGTGTTGGG GGATAAACTG
    GACGTGTCCT CCATCCCCGC TGACCCGTCA GGACTTCTGC CTTCACCTGA AGCCCTGAAA
    GGCAAAATAC TGATCAAGGG CAAGAAACTA CCCTCAAATA TAGATGAGAA CGCAGAGGAG
    GGGGACGTGT CTGATGAAGA CAGTGCAGAT GAGATGGAGG ACGATTGTAA GCTGTTGAAT
    GGAGATACGT CTATGAACCG AAAGCAGGCG GAGAATGTGG CCAAGAAAAA ATTAGATAAC
    CTCATGAAGG AGTCAAAGAT TCGCGACCGG GAAGATCCAG ACAGTTTTAC CATCACAGCC
    CTGCCACCGT CAGGAAAACA TGACAAATCG CCACTGAAGG GAAAAGCTGA AGATAGTACC
    GACACTGGAG ATGAGGCCAA CATCGGTGGA AACAAACGTC TGGCCAGAAC CTTCATGGGC
    AGCTTTTCCA AACGCAAGAA AAAGACTAGC AAAGTGAAGA AATCCTCTAG TTTTGAGGAC
    ACCGACACCG ATCAGGAGAG CTCAAGCGGG GCATCTAAAG CACAAGCACA TCACAGCAGG
    AAGAAAAAAA CCATGAAGCT GTCAAGAGCC CTTTCTGACC TGGTATACAT GAAATCTGTG
    GGAATGCCAG ACTTTGAGTC TCAAGGTGTC ACTTGGCAGG TGTCGTCGAT GAGCGAGACC
    AAAGCTCATC AGTTAATCCA GCAGAAGCCG GTGCCGTTCA TGCGGTTCAA CCAGCGGCAG
    CTCTCTCGCG TCTACCCATC TCCTTACCGC GTGGACTCCA GCAACTTCAA CCCACAGCCC
    TTCTGGAACG CTGGCTGCCA TCTGGTTGCG CTGAATTACC AGTCAGAGGG AAGAGTGCTG
    CAGCTCAACA GAGCTAAGTT CTATTGCAAT GGCAACTGTG GTTACATTCA GAAGCCTGGC
    TGCTTGTGTG AAGGTTCCTT TAATCCAGTA GCTGAGGATC CTTTGCCAGG TCGATTGAAA
    AAGCAACTGA TTCTTAAGGT CATCAGTGGT CAGCAATTGC CCAAACCAAA AGACTCCATG
    CTGGGGGACA GAGGAGAGAT AATCGATCCG TTTGTGGAAG TGGAGATTAT TGGGCTACCG
    GTGGATTGCT GCAAAGAGCA AACCAGAGTG GTTGACGACA ATGGATTCAA TCCTATGTGG
    GAAGAAACTC TCGTCTTCAC TCTGCACATG CCAGAGAACG CTCTTATACG CTTCCTGGTG
    TGGGATCATG ATCCGATAGG CCAAGATTTC ATTGGTCAGA GGACCATCGC CTTTAACAGC
    ATGATTCCCG GTTACCGCCA TGTGTATTTG GAAGGCATGG AGGAAGCTTC TATCTTTGTT
    CATGTTGCAG TGAATGACAT CACCAGTAAG GCTCGAGCAG TCAGTGGTAT AAAAGGACTC
    TTTCACAGAA ACCCAAAGCA AGCATCTCTC GACAGCCATG CTGCTGCTCT GCTCAACCGT
    AAGAGTACCT TCGGTGCTCA CCTCCTGCGC CGCACAGCCA GTGCCCCCAC CAAGGGCCAA
    CCCAAAGTCA AGAGGGGCTT CCCCGAGATC TCCATTGACA CAAAAGATTG CAGCTCGGAC
    GGCGCCAGCG AGGAGCGGGA ATCTGAAGAG GGGCCTCTTG TCCACCACAA TGGAGATACT
    GCGTCTGCAA AGTCATGGGG TCAAGGCACC AACGGGCAGC TTCACCCTGA TGACAGAAAA
    GTGCAAAACT GA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa60125
    Clone ID Related Accession (Same CDS sequence) XM_017357540.1 , NM_001347692.1 , XM_017357540.2
    Accession Version NM_001347692.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4605bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2019-01-26
    Organism Danio rerio(zebrafish)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2
    Comment Comment: VALIDATED REFSEQ: This record has undergone validation or preliminary review. The reference sequence was derived from CU570796.7, LO017720.1 and CU499311.14. On Dec 8, 2016 this sequence version replaced XM_017357541.1. Sequence Note: The RefSeq transcript and protein were derived from genomic sequence to make the sequence consistent with the reference genome assembly. The genomic coordinates used for the transcript record were based on alignments. ##Evidence-Data-START## Transcript exon combination :: GDQH01006232.1, GFIL01021359.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMEA2168446, SAMEA2168447 [ECO:0000348] ##Evidence-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGATACAG CATCCTTGAA CACCTCGCTG TTCTCCGGCA GCATGAGTAC GGATTCTCCC 
    AATTTGTCCA CGAGATCCAT GGGCTTCACT ACTGACACGG ATTCATCCCG CAGCAGCACA
    CTCTTACACT GCAATCATTT CTTGGCAGCC AACTACATCA GTGTGGTGGA AAAATGCATG
    AGTTCAATGC AGACGGGCAC TCAGATGGTA AAACTCCGTG GAGGATCCAA AGGCCTGGTG
    CGCTTTTTCT ACTTGGATGA ACACAAGTCC TGTATCCGCT GGCGACCGTC TAGGAAGAAT
    GAGAAAGCCA AAATCTCGAT CGACTCCATT CGGGAGGTTT GTGAGGGGAA GCAGTCTGAG
    ATCTTCCAGC GGTATTCAGA CGGTAGCTTT GATCCCAACT GCTGCTTCAG CATCTACTAT
    GGCGAGCACA TGGAGTCACT TGACCTGGTG TCTGGCACAG GAGAAGAGGC TCGCACCTGG
    ATTACTGGCC TTAAGTACCT TATGGCCGGC ATCAGCGATG AAGACAGTCT GGCTAAACGA
    CAACGCACTC GTGACCAGTG GCTGAAGCAG ACGTTTGCAG AGGCTGATAA GAATGGAGAC
    GGCTGCCTCA GCATCAGTGA AGTCCTCCAG CTCCTGCACA AGCTCAACGT CAACCTGCCC
    AGGCAGAAGG TCAAGCAGAT GTTTAAGGAA GCAGACACTG ATGACAACCA GGGTACTTTG
    GGATTTGAGG AGTTCTGCTC TTTCTATAAG ATGATGTCAA CGCGGCGTGA TCTGTACCTG
    CTGATGCTCA CCTACAGCAA TCATAAAGAC CACCTGGACA CTGATGACCT CAAGCGCTTC
    CTCGAGACGG AACAAAAGAT GACAAATGTC TCTAAGGAGC ACTGTCTTGA AATCGTGGCC
    AAGTTTGAAC CTTGTCCTGA AAACCAGACA TCGGGTGTGC TGGGGATTGA TGGTTTTACA
    AACTACATGC GCAGCCCTGC GGGGGACATC TTCAACCCTG AACATTACGA GGTGAACCAA
    GACATGACGC AGCCCCTCAG TGATTACTTC ATCGCTTCCT CCCACAACAC GTATCTGATG
    GGAGACCAGC TCATGTCTCA GTCTCGGGTT GATATGTACG CCTGGGTGCT GCAGGCCGGA
    TGCAGATGTG TGGAAGTGGA TTGCTGGGAT GGACAGGATG GAGAACCAAT CGTGCATCAT
    GGCTACACCC TGACTTCCAA AATCCTTTTT AAAGACGTCA TTGAAACCAT CAACAAATAC
    GCATTCGCCA AAAACCAGTA TCCAGTGATT CTGTCCATCG AGAATCACTG CAGCGTCCCT
    CAGCAAAAGA AAATGGCTCA GTATCTGACT GAAGTGTTGG GGGATAAACT GGACGTGTCC
    TCCATCCCCG CTGACCCGTC AGGACTTCTG CCTTCACCTG AAGCCCTGAA AGGCAAAATA
    CTGATCAAGG GCAAGAAACT ACCCTCAAAT ATAGATGAGA ACGCAGAGGA GGGGGACGTG
    TCTGATGAAG ACAGTGCAGA TGAGATGGAG GACGATTGTA AGCTGTTGAA TGGAGATACG
    TCTATGAACC GAAAGCAGGC GGAGAATGTG GCCAAGAAAA AATTAGATAA CCTCATGAAG
    GAGTCAAAGA TTCGCGACCG GGAAGATCCA GACAGTTTTA CCATCACAGC CCTGCCACCG
    TCAGGAAAAC ATGACAAATC GCCACTGAAG GGAAAAGCTG AAGATAGTAC CGACACTGGA
    GATGAGGCCA ACATCGGTGG AAACAAACGT CTGGCCAGAA CCTTCATGGG CAGCTTTTCC
    AAACGCAAGA AAAAGACTAG CAAAGTGAAG AAATCCTCTA GTTTTGAGGA CACCGACACC
    GATCAGGAGA GCTCAAGCGG GGCATCTAAA GCACAAGCAC ATCACAGCAG GAAGAAAAAA
    ACCATGAAGC TGTCAAGAGC CCTTTCTGAC CTGGTATACA TGAAATCTGT GGGAATGCCA
    GACTTTGAGT CTCAAGGTGT CACTTGGCAG GTGTCGTCGA TGAGCGAGAC CAAAGCTCAT
    CAGTTAATCC AGCAGAAGCC GGTGCCGTTC ATGCGGTTCA ACCAGCGGCA GCTCTCTCGC
    GTCTACCCAT CTCCTTACCG CGTGGACTCC AGCAACTTCA ACCCACAGCC CTTCTGGAAC
    GCTGGCTGCC ATCTGGTTGC GCTGAATTAC CAGTCAGAGG GAAGAGTGCT GCAGCTCAAC
    AGAGCTAAGT TCTATTGCAA TGGCAACTGT GGTTACATTC AGAAGCCTGG CTGCTTGTGT
    GAAGGTTCCT TTAATCCAGT AGCTGAGGAT CCTTTGCCAG GTCGATTGAA AAAGCAACTG
    ATTCTTAAGG TCATCAGTGG TCAGCAATTG CCCAAACCAA AAGACTCCAT GCTGGGGGAC
    AGAGGAGAGA TAATCGATCC GTTTGTGGAA GTGGAGATTA TTGGGCTACC GGTGGATTGC
    TGCAAAGAGC AAACCAGAGT GGTTGACGAC AATGGATTCA ATCCTATGTG GGAAGAAACT
    CTCGTCTTCA CTCTGCACAT GCCAGAGAAC GCTCTTATAC GCTTCCTGGT GTGGGATCAT
    GATCCGATAG GCCAAGATTT CATTGGTCAG AGGACCATCG CCTTTAACAG CATGATTCCC
    GGTTACCGCC ATGTGTATTT GGAAGGCATG GAGGAAGCTT CTATCTTTGT TCATGTTGCA
    GTGAATGACA TCACCAGTAA GGCTCGAGCA GTCAGTGGTA TAAAAGGACT CTTTCACAGA
    AACCCAAAGC AAGCATCTCT CGACAGCCAT GCTGCTGCTC TGCTCAACCG TAAGAGTACC
    TTCGGTGCTC ACCTCCTGCG CCGCACAGCC AGTGCCCCCA CCAAGGGCCA ACCCAAAGTC
    AAGAGGGGCT TCCCCGAGAT CTCCATTGAC ACAAAAGATT GCAGCTCGGA CGGCGCCAGC
    GAGGAGCGGG AATCTGAAGA GGGGCCTCTT GTCCACCACA ATGGAGATAC TGCGTCTGCA
    AAGTCATGGG GTCAAGGCAC CAACGGGCAG CTTCACCCTG ATGACAGAAA AGAAAATTCT
    GAAGTGCAGA GTCCAACTCC ATCTCTGAAC GCCTCATGTC TGGAATCCAT CACTGAGAAC
    TTTCAGGAGA ACAGCCCTTC GACCTCAACG CAAAGTCCTG TGGATACCTC ACCGCATTGT
    CCCTTGTCCT CACCATCCAC AGCAATAACG GCATCTTCCA ACGGGGAAAA TCTCAGCCCT
    GTGAAAAATA ACGAGGACAT CCTGAAATCA TTTGCACTAA CCCAATCTAC GAGTGGTAAG
    GATTCTGGGA ACAGTCCTGT AAACTCATCA ATTTCCACTA ATTACCTTGA CATTGGATGC
    GACACGCCAA CTTTTCCCAC TTTCCATCGG GCGGATGGAG AGGTTGCACA AGAGACAGTC
    ATCAATCAAA CAGGACTGAC TCTAGAGATA GATGCTGGGA AGGAACCAAC TGCCATTCAG
    AGCTCTCAGA GGATTCAGGC ATTGAAGGCA GTGTTTGACA GAGACATTGG ACGAAGCATG
    CAAACAGGAC TGAGGACCCC TGTGAGAAGG TCCAAAAGTG AAGGACAAAT GAAAAAGGCA
    GACGGCCCTT CGGTGGTGCC AGAAGTTTGT ACTGACGCAA CTTTAAACGA CAGGCTCTGG
    AGCAAACTTG ACCCCAGCAG CCATCGGGAT AGTGTGTCAT CGTCTTCCAG CATTTCGTCC
    AATGACACTG TGATTGATCT GTCCTTGCCC AACCTTTCCA GGAAAAGCCT TACCTGTCTT
    AGCGCAGCCC GGGATTATTC AGACAAGCAG GCTTCGTTGA CCAACAACCA TCGGTCGGTC
    ATTGCAGGAT GTGGAGTCTC GCTTGTCAGC AAGAGCAAGT CCAACCCCAA CCTGCGGGAT
    GGCGAGATGT GCAATACAGA TGACGATTTG CAACCCAGAC CTCTGGTAGC CGATAAAGAC
    CCCATCCGAC CCATGCAAAG GCGACACACG TGGAGCAGGT TGTACGTTGA AGGCCTAAAG
    CAGTCATCGA CACTTGCTGC TTCTGCAGCC TCCAAGAAGT CAACTACCCT TTCAGCAAAA
    GACATCGACT CAAAGTCCAA AAGTCTGGGA GATCTCACTT CAGACGATAT CATCTGTAAC
    TTCGAGAGCA AGTATCGCAG CATTAGCCGC AGTTTCATCG TGAGGCCAAG TCGCCAACAT
    GGGAAAACTC TGAGGAAACC CCAAAATGAT TTCACTGAGC AGCTGAAGAG GCTCACTGAC
    GTGGAACCCC TTACCAGCAA GGACTTTGCT CAGTGCCGGG CTGACTCAGA AGCAGGACAG
    GAAGAAGAGG AGATGCCTCC TTTACGGAGG AGTTCATCAC GGAGCCAGAG CCGCGTGAGA
    TACATCGCTA ACCGTGCCAG GCAGGCCCAG GAAAGGCAAC GGCTACAAAG CATAACCCGG
    ATGTCTGGGA GCCCCATCGA AGAGCGTGGG AACCCTGAAG GAGCTTGTAG TGTACCCCAT
    AGTCCTTGCA CTTGCCTGGA TCTTTTAAGC CAGCTTCCAC CAGGACCACC CCAACAAAGC
    CCACTGAGCC CGGACAATGA GGTCTTCTTT ATGCTCAGGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq NP_001334621.1
    CDS483..5087
    Translation

    Target ORF information:

    RefSeq Version NM_001347692.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio phospholipase C, eta 2a (plch2a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    NM_001347692.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGATACAG CATCCTTGAA CACCTCGCTG TTCTCCGGCA GCATGAGTAC GGATTCTCCC 
    AATTTGTCCA CGAGATCCAT GGGCTTCACT ACTGACACGG ATTCATCCCG CAGCAGCACA
    CTCTTACACT GCAATCATTT CTTGGCAGCC AACTACATCA GTGTGGTGGA AAAATGCATG
    AGTTCAATGC AGACGGGCAC TCAGATGGTA AAACTCCGTG GAGGATCCAA AGGCCTGGTG
    CGCTTTTTCT ACTTGGATGA ACACAAGTCC TGTATCCGCT GGCGACCGTC TAGGAAGAAT
    GAGAAAGCCA AAATCTCGAT CGACTCCATT CGGGAGGTTT GTGAGGGGAA GCAGTCTGAG
    ATCTTCCAGC GGTATTCAGA CGGTAGCTTT GATCCCAACT GCTGCTTCAG CATCTACTAT
    GGCGAGCACA TGGAGTCACT TGACCTGGTG TCTGGCACAG GAGAAGAGGC TCGCACCTGG
    ATTACTGGCC TTAAGTACCT TATGGCCGGC ATCAGCGATG AAGACAGTCT GGCTAAACGA
    CAACGCACTC GTGACCAGTG GCTGAAGCAG ACGTTTGCAG AGGCTGATAA GAATGGAGAC
    GGCTGCCTCA GCATCAGTGA AGTCCTCCAG CTCCTGCACA AGCTCAACGT CAACCTGCCC
    AGGCAGAAGG TCAAGCAGAT GTTTAAGGAA GCAGACACTG ATGACAACCA GGGTACTTTG
    GGATTTGAGG AGTTCTGCTC TTTCTATAAG ATGATGTCAA CGCGGCGTGA TCTGTACCTG
    CTGATGCTCA CCTACAGCAA TCATAAAGAC CACCTGGACA CTGATGACCT CAAGCGCTTC
    CTCGAGACGG AACAAAAGAT GACAAATGTC TCTAAGGAGC ACTGTCTTGA AATCGTGGCC
    AAGTTTGAAC CTTGTCCTGA AAACCAGACA TCGGGTGTGC TGGGGATTGA TGGTTTTACA
    AACTACATGC GCAGCCCTGC GGGGGACATC TTCAACCCTG AACATTACGA GGTGAACCAA
    GACATGACGC AGCCCCTCAG TGATTACTTC ATCGCTTCCT CCCACAACAC GTATCTGATG
    GGAGACCAGC TCATGTCTCA GTCTCGGGTT GATATGTACG CCTGGGTGCT GCAGGCCGGA
    TGCAGATGTG TGGAAGTGGA TTGCTGGGAT GGACAGGATG GAGAACCAAT CGTGCATCAT
    GGCTACACCC TGACTTCCAA AATCCTTTTT AAAGACGTCA TTGAAACCAT CAACAAATAC
    GCATTCGCCA AAAACCAGTA TCCAGTGATT CTGTCCATCG AGAATCACTG CAGCGTCCCT
    CAGCAAAAGA AAATGGCTCA GTATCTGACT GAAGTGTTGG GGGATAAACT GGACGTGTCC
    TCCATCCCCG CTGACCCGTC AGGACTTCTG CCTTCACCTG AAGCCCTGAA AGGCAAAATA
    CTGATCAAGG GCAAGAAACT ACCCTCAAAT ATAGATGAGA ACGCAGAGGA GGGGGACGTG
    TCTGATGAAG ACAGTGCAGA TGAGATGGAG GACGATTGTA AGCTGTTGAA TGGAGATACG
    TCTATGAACC GAAAGCAGGC GGAGAATGTG GCCAAGAAAA AATTAGATAA CCTCATGAAG
    GAGTCAAAGA TTCGCGACCG GGAAGATCCA GACAGTTTTA CCATCACAGC CCTGCCACCG
    TCAGGAAAAC ATGACAAATC GCCACTGAAG GGAAAAGCTG AAGATAGTAC CGACACTGGA
    GATGAGGCCA ACATCGGTGG AAACAAACGT CTGGCCAGAA CCTTCATGGG CAGCTTTTCC
    AAACGCAAGA AAAAGACTAG CAAAGTGAAG AAATCCTCTA GTTTTGAGGA CACCGACACC
    GATCAGGAGA GCTCAAGCGG GGCATCTAAA GCACAAGCAC ATCACAGCAG GAAGAAAAAA
    ACCATGAAGC TGTCAAGAGC CCTTTCTGAC CTGGTATACA TGAAATCTGT GGGAATGCCA
    GACTTTGAGT CTCAAGGTGT CACTTGGCAG GTGTCGTCGA TGAGCGAGAC CAAAGCTCAT
    CAGTTAATCC AGCAGAAGCC GGTGCCGTTC ATGCGGTTCA ACCAGCGGCA GCTCTCTCGC
    GTCTACCCAT CTCCTTACCG CGTGGACTCC AGCAACTTCA ACCCACAGCC CTTCTGGAAC
    GCTGGCTGCC ATCTGGTTGC GCTGAATTAC CAGTCAGAGG GAAGAGTGCT GCAGCTCAAC
    AGAGCTAAGT TCTATTGCAA TGGCAACTGT GGTTACATTC AGAAGCCTGG CTGCTTGTGT
    GAAGGTTCCT TTAATCCAGT AGCTGAGGAT CCTTTGCCAG GTCGATTGAA AAAGCAACTG
    ATTCTTAAGG TCATCAGTGG TCAGCAATTG CCCAAACCAA AAGACTCCAT GCTGGGGGAC
    AGAGGAGAGA TAATCGATCC GTTTGTGGAA GTGGAGATTA TTGGGCTACC GGTGGATTGC
    TGCAAAGAGC AAACCAGAGT GGTTGACGAC AATGGATTCA ATCCTATGTG GGAAGAAACT
    CTCGTCTTCA CTCTGCACAT GCCAGAGAAC GCTCTTATAC GCTTCCTGGT GTGGGATCAT
    GATCCGATAG GCCAAGATTT CATTGGTCAG AGGACCATCG CCTTTAACAG CATGATTCCC
    GGTTACCGCC ATGTGTATTT GGAAGGCATG GAGGAAGCTT CTATCTTTGT TCATGTTGCA
    GTGAATGACA TCACCAGTAA GGCTCGAGCA GTCAGTGGTA TAAAAGGACT CTTTCACAGA
    AACCCAAAGC AAGCATCTCT CGACAGCCAT GCTGCTGCTC TGCTCAACCG TAAGAGTACC
    TTCGGTGCTC ACCTCCTGCG CCGCACAGCC AGTGCCCCCA CCAAGGGCCA ACCCAAAGTC
    AAGAGGGGCT TCCCCGAGAT CTCCATTGAC ACAAAAGATT GCAGCTCGGA CGGCGCCAGC
    GAGGAGCGGG AATCTGAAGA GGGGCCTCTT GTCCACCACA ATGGAGATAC TGCGTCTGCA
    AAGTCATGGG GTCAAGGCAC CAACGGGCAG CTTCACCCTG ATGACAGAAA AGAAAATTCT
    GAAGTGCAGA GTCCAACTCC ATCTCTGAAC GCCTCATGTC TGGAATCCAT CACTGAGAAC
    TTTCAGGAGA ACAGCCCTTC GACCTCAACG CAAAGTCCTG TGGATACCTC ACCGCATTGT
    CCCTTGTCCT CACCATCCAC AGCAATAACG GCATCTTCCA ACGGGGAAAA TCTCAGCCCT
    GTGAAAAATA ACGAGGACAT CCTGAAATCA TTTGCACTAA CCCAATCTAC GAGTGGTAAG
    GATTCTGGGA ACAGTCCTGT AAACTCATCA ATTTCCACTA ATTACCTTGA CATTGGATGC
    GACACGCCAA CTTTTCCCAC TTTCCATCGG GCGGATGGAG AGGTTGCACA AGAGACAGTC
    ATCAATCAAA CAGGACTGAC TCTAGAGATA GATGCTGGGA AGGAACCAAC TGCCATTCAG
    AGCTCTCAGA GGATTCAGGC ATTGAAGGCA GTGTTTGACA GAGACATTGG ACGAAGCATG
    CAAACAGGAC TGAGGACCCC TGTGAGAAGG TCCAAAAGTG AAGGACAAAT GAAAAAGGCA
    GACGGCCCTT CGGTGGTGCC AGAAGTTTGT ACTGACGCAA CTTTAAACGA CAGGCTCTGG
    AGCAAACTTG ACCCCAGCAG CCATCGGGAT AGTGTGTCAT CGTCTTCCAG CATTTCGTCC
    AATGACACTG TGATTGATCT GTCCTTGCCC AACCTTTCCA GGAAAAGCCT TACCTGTCTT
    AGCGCAGCCC GGGATTATTC AGACAAGCAG GCTTCGTTGA CCAACAACCA TCGGTCGGTC
    ATTGCAGGAT GTGGAGTCTC GCTTGTCAGC AAGAGCAAGT CCAACCCCAA CCTGCGGGAT
    GGCGAGATGT GCAATACAGA TGACGATTTG CAACCCAGAC CTCTGGTAGC CGATAAAGAC
    CCCATCCGAC CCATGCAAAG GCGACACACG TGGAGCAGGT TGTACGTTGA AGGCCTAAAG
    CAGTCATCGA CACTTGCTGC TTCTGCAGCC TCCAAGAAGT CAACTACCCT TTCAGCAAAA
    GACATCGACT CAAAGTCCAA AAGTCTGGGA GATCTCACTT CAGACGATAT CATCTGTAAC
    TTCGAGAGCA AGTATCGCAG CATTAGCCGC AGTTTCATCG TGAGGCCAAG TCGCCAACAT
    GGGAAAACTC TGAGGAAACC CCAAAATGAT TTCACTGAGC AGCTGAAGAG GCTCACTGAC
    GTGGAACCCC TTACCAGCAA GGACTTTGCT CAGTGCCGGG CTGACTCAGA AGCAGGACAG
    GAAGAAGAGG AGATGCCTCC TTTACGGAGG AGTTCATCAC GGAGCCAGAG CCGCGTGAGA
    TACATCGCTA ACCGTGCCAG GCAGGCCCAG GAAAGGCAAC GGCTACAAAG CATAACCCGG
    ATGTCTGGGA GCCCCATCGA AGAGCGTGGG AACCCTGAAG GAGCTTGTAG TGTACCCCAT
    AGTCCTTGCA CTTGCCTGGA TCTTTTAAGC CAGCTTCCAC CAGGACCACC CCAACAAAGC
    CCACTGAGCC CGGACAATGA GGTCTTCTTT ATGCTCAGGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa60125
    Clone ID Related Accession (Same CDS sequence) XM_017357540.1 , NM_001347692.1 , XM_017357540.2
    Accession Version XM_017357540.1 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4605bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGATACAG CATCCTTGAA CACCTCGCTG TTCTCCGGCA GCATGAGTAC GGATTCTCCC 
    AATTTGTCCA CGAGATCCAT GGGCTTCACT ACTGACACGG ATTCATCCCG CAGCAGCACA
    CTCTTACACT GCAATCATTT CTTGGCAGCC AACTACATCA GTGTGGTGGA AAAATGCATG
    AGTTCAATGC AGACGGGCAC TCAGATGGTA AAACTCCGTG GAGGATCCAA AGGCCTGGTG
    CGCTTTTTCT ACTTGGATGA ACACAAGTCC TGTATCCGCT GGCGACCGTC TAGGAAGAAT
    GAGAAAGCCA AAATCTCGAT CGACTCCATT CGGGAGGTTT GTGAGGGGAA GCAGTCTGAG
    ATCTTCCAGC GGTATTCAGA CGGTAGCTTT GATCCCAACT GCTGCTTCAG CATCTACTAT
    GGCGAGCACA TGGAGTCACT TGACCTGGTG TCTGGCACAG GAGAAGAGGC TCGCACCTGG
    ATTACTGGCC TTAAGTACCT TATGGCCGGC ATCAGCGATG AAGACAGTCT GGCTAAACGA
    CAACGCACTC GTGACCAGTG GCTGAAGCAG ACGTTTGCAG AGGCTGATAA GAATGGAGAC
    GGCTGCCTCA GCATCAGTGA AGTCCTCCAG CTCCTGCACA AGCTCAACGT CAACCTGCCC
    AGGCAGAAGG TCAAGCAGAT GTTTAAGGAA GCAGACACTG ATGACAACCA GGGTACTTTG
    GGATTTGAGG AGTTCTGCTC TTTCTATAAG ATGATGTCAA CGCGGCGTGA TCTGTACCTG
    CTGATGCTCA CCTACAGCAA TCATAAAGAC CACCTGGACA CTGATGACCT CAAGCGCTTC
    CTCGAGACGG AACAAAAGAT GACAAATGTC TCTAAGGAGC ACTGTCTTGA AATCGTGGCC
    AAGTTTGAAC CTTGTCCTGA AAACCAGACA TCGGGTGTGC TGGGGATTGA TGGTTTTACA
    AACTACATGC GCAGCCCTGC GGGGGACATC TTCAACCCTG AACATTACGA GGTGAACCAA
    GACATGACGC AGCCCCTCAG TGATTACTTC ATCGCTTCCT CCCACAACAC GTATCTGATG
    GGAGACCAGC TCATGTCTCA GTCTCGGGTT GATATGTACG CCTGGGTGCT GCAGGCCGGA
    TGCAGATGTG TGGAAGTGGA TTGCTGGGAT GGACAGGATG GAGAACCAAT CGTGCATCAT
    GGCTACACCC TGACTTCCAA AATCCTTTTT AAAGACGTCA TTGAAACCAT CAACAAATAC
    GCATTCGCCA AAAACCAGTA TCCAGTGATT CTGTCCATCG AGAATCACTG CAGCGTCCCT
    CAGCAAAAGA AAATGGCTCA GTATCTGACT GAAGTGTTGG GGGATAAACT GGACGTGTCC
    TCCATCCCCG CTGACCCGTC AGGACTTCTG CCTTCACCTG AAGCCCTGAA AGGCAAAATA
    CTGATCAAGG GCAAGAAACT ACCCTCAAAT ATAGATGAGA ACGCAGAGGA GGGGGACGTG
    TCTGATGAAG ACAGTGCAGA TGAGATGGAG GACGATTGTA AGCTGTTGAA TGGAGATACG
    TCTATGAACC GAAAGCAGGC GGAGAATGTG GCCAAGAAAA AATTAGATAA CCTCATGAAG
    GAGTCAAAGA TTCGCGACCG GGAAGATCCA GACAGTTTTA CCATCACAGC CCTGCCACCG
    TCAGGAAAAC ATGACAAATC GCCACTGAAG GGAAAAGCTG AAGATAGTAC CGACACTGGA
    GATGAGGCCA ACATCGGTGG AAACAAACGT CTGGCCAGAA CCTTCATGGG CAGCTTTTCC
    AAACGCAAGA AAAAGACTAG CAAAGTGAAG AAATCCTCTA GTTTTGAGGA CACCGACACC
    GATCAGGAGA GCTCAAGCGG GGCATCTAAA GCACAAGCAC ATCACAGCAG GAAGAAAAAA
    ACCATGAAGC TGTCAAGAGC CCTTTCTGAC CTGGTATACA TGAAATCTGT GGGAATGCCA
    GACTTTGAGT CTCAAGGTGT CACTTGGCAG GTGTCGTCGA TGAGCGAGAC CAAAGCTCAT
    CAGTTAATCC AGCAGAAGCC GGTGCCGTTC ATGCGGTTCA ACCAGCGGCA GCTCTCTCGC
    GTCTACCCAT CTCCTTACCG CGTGGACTCC AGCAACTTCA ACCCACAGCC CTTCTGGAAC
    GCTGGCTGCC ATCTGGTTGC GCTGAATTAC CAGTCAGAGG GAAGAGTGCT GCAGCTCAAC
    AGAGCTAAGT TCTATTGCAA TGGCAACTGT GGTTACATTC AGAAGCCTGG CTGCTTGTGT
    GAAGGTTCCT TTAATCCAGT AGCTGAGGAT CCTTTGCCAG GTCGATTGAA AAAGCAACTG
    ATTCTTAAGG TCATCAGTGG TCAGCAATTG CCCAAACCAA AAGACTCCAT GCTGGGGGAC
    AGAGGAGAGA TAATCGATCC GTTTGTGGAA GTGGAGATTA TTGGGCTACC GGTGGATTGC
    TGCAAAGAGC AAACCAGAGT GGTTGACGAC AATGGATTCA ATCCTATGTG GGAAGAAACT
    CTCGTCTTCA CTCTGCACAT GCCAGAGAAC GCTCTTATAC GCTTCCTGGT GTGGGATCAT
    GATCCGATAG GCCAAGATTT CATTGGTCAG AGGACCATCG CCTTTAACAG CATGATTCCC
    GGTTACCGCC ATGTGTATTT GGAAGGCATG GAGGAAGCTT CTATCTTTGT TCATGTTGCA
    GTGAATGACA TCACCAGTAA GGCTCGAGCA GTCAGTGGTA TAAAAGGACT CTTTCACAGA
    AACCCAAAGC AAGCATCTCT CGACAGCCAT GCTGCTGCTC TGCTCAACCG TAAGAGTACC
    TTCGGTGCTC ACCTCCTGCG CCGCACAGCC AGTGCCCCCA CCAAGGGCCA ACCCAAAGTC
    AAGAGGGGCT TCCCCGAGAT CTCCATTGAC ACAAAAGATT GCAGCTCGGA CGGCGCCAGC
    GAGGAGCGGG AATCTGAAGA GGGGCCTCTT GTCCACCACA ATGGAGATAC TGCGTCTGCA
    AAGTCATGGG GTCAAGGCAC CAACGGGCAG CTTCACCCTG ATGACAGAAA AGAAAATTCT
    GAAGTGCAGA GTCCAACTCC ATCTCTGAAC GCCTCATGTC TGGAATCCAT CACTGAGAAC
    TTTCAGGAGA ACAGCCCTTC GACCTCAACG CAAAGTCCTG TGGATACCTC ACCGCATTGT
    CCCTTGTCCT CACCATCCAC AGCAATAACG GCATCTTCCA ACGGGGAAAA TCTCAGCCCT
    GTGAAAAATA ACGAGGACAT CCTGAAATCA TTTGCACTAA CCCAATCTAC GAGTGGTAAG
    GATTCTGGGA ACAGTCCTGT AAACTCATCA ATTTCCACTA ATTACCTTGA CATTGGATGC
    GACACGCCAA CTTTTCCCAC TTTCCATCGG GCGGATGGAG AGGTTGCACA AGAGACAGTC
    ATCAATCAAA CAGGACTGAC TCTAGAGATA GATGCTGGGA AGGAACCAAC TGCCATTCAG
    AGCTCTCAGA GGATTCAGGC ATTGAAGGCA GTGTTTGACA GAGACATTGG ACGAAGCATG
    CAAACAGGAC TGAGGACCCC TGTGAGAAGG TCCAAAAGTG AAGGACAAAT GAAAAAGGCA
    GACGGCCCTT CGGTGGTGCC AGAAGTTTGT ACTGACGCAA CTTTAAACGA CAGGCTCTGG
    AGCAAACTTG ACCCCAGCAG CCATCGGGAT AGTGTGTCAT CGTCTTCCAG CATTTCGTCC
    AATGACACTG TGATTGATCT GTCCTTGCCC AACCTTTCCA GGAAAAGCCT TACCTGTCTT
    AGCGCAGCCC GGGATTATTC AGACAAGCAG GCTTCGTTGA CCAACAACCA TCGGTCGGTC
    ATTGCAGGAT GTGGAGTCTC GCTTGTCAGC AAGAGCAAGT CCAACCCCAA CCTGCGGGAT
    GGCGAGATGT GCAATACAGA TGACGATTTG CAACCCAGAC CTCTGGTAGC CGATAAAGAC
    CCCATCCGAC CCATGCAAAG GCGACACACG TGGAGCAGGT TGTACGTTGA AGGCCTAAAG
    CAGTCATCGA CACTTGCTGC TTCTGCAGCC TCCAAGAAGT CAACTACCCT TTCAGCAAAA
    GACATCGACT CAAAGTCCAA AAGTCTGGGA GATCTCACTT CAGACGATAT CATCTGTAAC
    TTCGAGAGCA AGTATCGCAG CATTAGCCGC AGTTTCATCG TGAGGCCAAG TCGCCAACAT
    GGGAAAACTC TGAGGAAACC CCAAAATGAT TTCACTGAGC AGCTGAAGAG GCTCACTGAC
    GTGGAACCCC TTACCAGCAA GGACTTTGCT CAGTGCCGGG CTGACTCAGA AGCAGGACAG
    GAAGAAGAGG AGATGCCTCC TTTACGGAGG AGTTCATCAC GGAGCCAGAG CCGCGTGAGA
    TACATCGCTA ACCGTGCCAG GCAGGCCCAG GAAAGGCAAC GGCTACAAAG CATAACCCGG
    ATGTCTGGGA GCCCCATCGA AGAGCGTGGG AACCCTGAAG GAGCTTGTAG TGTACCCCAT
    AGTCCTTGCA CTTGCCTGGA TCTTTTAAGC CAGCTTCCAC CAGGACCACC CCAACAAAGC
    CCACTGAGCC CGGACAATGA GGTCTTCTTT ATGCTCAGGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017213029.1
    CDS388..4992
    Translation

    Target ORF information:

    RefSeq Version XM_017357540.1
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio phospholipase C eta 2 (plch2), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017357540.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGATACAG CATCCTTGAA CACCTCGCTG TTCTCCGGCA GCATGAGTAC GGATTCTCCC 
    AATTTGTCCA CGAGATCCAT GGGCTTCACT ACTGACACGG ATTCATCCCG CAGCAGCACA
    CTCTTACACT GCAATCATTT CTTGGCAGCC AACTACATCA GTGTGGTGGA AAAATGCATG
    AGTTCAATGC AGACGGGCAC TCAGATGGTA AAACTCCGTG GAGGATCCAA AGGCCTGGTG
    CGCTTTTTCT ACTTGGATGA ACACAAGTCC TGTATCCGCT GGCGACCGTC TAGGAAGAAT
    GAGAAAGCCA AAATCTCGAT CGACTCCATT CGGGAGGTTT GTGAGGGGAA GCAGTCTGAG
    ATCTTCCAGC GGTATTCAGA CGGTAGCTTT GATCCCAACT GCTGCTTCAG CATCTACTAT
    GGCGAGCACA TGGAGTCACT TGACCTGGTG TCTGGCACAG GAGAAGAGGC TCGCACCTGG
    ATTACTGGCC TTAAGTACCT TATGGCCGGC ATCAGCGATG AAGACAGTCT GGCTAAACGA
    CAACGCACTC GTGACCAGTG GCTGAAGCAG ACGTTTGCAG AGGCTGATAA GAATGGAGAC
    GGCTGCCTCA GCATCAGTGA AGTCCTCCAG CTCCTGCACA AGCTCAACGT CAACCTGCCC
    AGGCAGAAGG TCAAGCAGAT GTTTAAGGAA GCAGACACTG ATGACAACCA GGGTACTTTG
    GGATTTGAGG AGTTCTGCTC TTTCTATAAG ATGATGTCAA CGCGGCGTGA TCTGTACCTG
    CTGATGCTCA CCTACAGCAA TCATAAAGAC CACCTGGACA CTGATGACCT CAAGCGCTTC
    CTCGAGACGG AACAAAAGAT GACAAATGTC TCTAAGGAGC ACTGTCTTGA AATCGTGGCC
    AAGTTTGAAC CTTGTCCTGA AAACCAGACA TCGGGTGTGC TGGGGATTGA TGGTTTTACA
    AACTACATGC GCAGCCCTGC GGGGGACATC TTCAACCCTG AACATTACGA GGTGAACCAA
    GACATGACGC AGCCCCTCAG TGATTACTTC ATCGCTTCCT CCCACAACAC GTATCTGATG
    GGAGACCAGC TCATGTCTCA GTCTCGGGTT GATATGTACG CCTGGGTGCT GCAGGCCGGA
    TGCAGATGTG TGGAAGTGGA TTGCTGGGAT GGACAGGATG GAGAACCAAT CGTGCATCAT
    GGCTACACCC TGACTTCCAA AATCCTTTTT AAAGACGTCA TTGAAACCAT CAACAAATAC
    GCATTCGCCA AAAACCAGTA TCCAGTGATT CTGTCCATCG AGAATCACTG CAGCGTCCCT
    CAGCAAAAGA AAATGGCTCA GTATCTGACT GAAGTGTTGG GGGATAAACT GGACGTGTCC
    TCCATCCCCG CTGACCCGTC AGGACTTCTG CCTTCACCTG AAGCCCTGAA AGGCAAAATA
    CTGATCAAGG GCAAGAAACT ACCCTCAAAT ATAGATGAGA ACGCAGAGGA GGGGGACGTG
    TCTGATGAAG ACAGTGCAGA TGAGATGGAG GACGATTGTA AGCTGTTGAA TGGAGATACG
    TCTATGAACC GAAAGCAGGC GGAGAATGTG GCCAAGAAAA AATTAGATAA CCTCATGAAG
    GAGTCAAAGA TTCGCGACCG GGAAGATCCA GACAGTTTTA CCATCACAGC CCTGCCACCG
    TCAGGAAAAC ATGACAAATC GCCACTGAAG GGAAAAGCTG AAGATAGTAC CGACACTGGA
    GATGAGGCCA ACATCGGTGG AAACAAACGT CTGGCCAGAA CCTTCATGGG CAGCTTTTCC
    AAACGCAAGA AAAAGACTAG CAAAGTGAAG AAATCCTCTA GTTTTGAGGA CACCGACACC
    GATCAGGAGA GCTCAAGCGG GGCATCTAAA GCACAAGCAC ATCACAGCAG GAAGAAAAAA
    ACCATGAAGC TGTCAAGAGC CCTTTCTGAC CTGGTATACA TGAAATCTGT GGGAATGCCA
    GACTTTGAGT CTCAAGGTGT CACTTGGCAG GTGTCGTCGA TGAGCGAGAC CAAAGCTCAT
    CAGTTAATCC AGCAGAAGCC GGTGCCGTTC ATGCGGTTCA ACCAGCGGCA GCTCTCTCGC
    GTCTACCCAT CTCCTTACCG CGTGGACTCC AGCAACTTCA ACCCACAGCC CTTCTGGAAC
    GCTGGCTGCC ATCTGGTTGC GCTGAATTAC CAGTCAGAGG GAAGAGTGCT GCAGCTCAAC
    AGAGCTAAGT TCTATTGCAA TGGCAACTGT GGTTACATTC AGAAGCCTGG CTGCTTGTGT
    GAAGGTTCCT TTAATCCAGT AGCTGAGGAT CCTTTGCCAG GTCGATTGAA AAAGCAACTG
    ATTCTTAAGG TCATCAGTGG TCAGCAATTG CCCAAACCAA AAGACTCCAT GCTGGGGGAC
    AGAGGAGAGA TAATCGATCC GTTTGTGGAA GTGGAGATTA TTGGGCTACC GGTGGATTGC
    TGCAAAGAGC AAACCAGAGT GGTTGACGAC AATGGATTCA ATCCTATGTG GGAAGAAACT
    CTCGTCTTCA CTCTGCACAT GCCAGAGAAC GCTCTTATAC GCTTCCTGGT GTGGGATCAT
    GATCCGATAG GCCAAGATTT CATTGGTCAG AGGACCATCG CCTTTAACAG CATGATTCCC
    GGTTACCGCC ATGTGTATTT GGAAGGCATG GAGGAAGCTT CTATCTTTGT TCATGTTGCA
    GTGAATGACA TCACCAGTAA GGCTCGAGCA GTCAGTGGTA TAAAAGGACT CTTTCACAGA
    AACCCAAAGC AAGCATCTCT CGACAGCCAT GCTGCTGCTC TGCTCAACCG TAAGAGTACC
    TTCGGTGCTC ACCTCCTGCG CCGCACAGCC AGTGCCCCCA CCAAGGGCCA ACCCAAAGTC
    AAGAGGGGCT TCCCCGAGAT CTCCATTGAC ACAAAAGATT GCAGCTCGGA CGGCGCCAGC
    GAGGAGCGGG AATCTGAAGA GGGGCCTCTT GTCCACCACA ATGGAGATAC TGCGTCTGCA
    AAGTCATGGG GTCAAGGCAC CAACGGGCAG CTTCACCCTG ATGACAGAAA AGAAAATTCT
    GAAGTGCAGA GTCCAACTCC ATCTCTGAAC GCCTCATGTC TGGAATCCAT CACTGAGAAC
    TTTCAGGAGA ACAGCCCTTC GACCTCAACG CAAAGTCCTG TGGATACCTC ACCGCATTGT
    CCCTTGTCCT CACCATCCAC AGCAATAACG GCATCTTCCA ACGGGGAAAA TCTCAGCCCT
    GTGAAAAATA ACGAGGACAT CCTGAAATCA TTTGCACTAA CCCAATCTAC GAGTGGTAAG
    GATTCTGGGA ACAGTCCTGT AAACTCATCA ATTTCCACTA ATTACCTTGA CATTGGATGC
    GACACGCCAA CTTTTCCCAC TTTCCATCGG GCGGATGGAG AGGTTGCACA AGAGACAGTC
    ATCAATCAAA CAGGACTGAC TCTAGAGATA GATGCTGGGA AGGAACCAAC TGCCATTCAG
    AGCTCTCAGA GGATTCAGGC ATTGAAGGCA GTGTTTGACA GAGACATTGG ACGAAGCATG
    CAAACAGGAC TGAGGACCCC TGTGAGAAGG TCCAAAAGTG AAGGACAAAT GAAAAAGGCA
    GACGGCCCTT CGGTGGTGCC AGAAGTTTGT ACTGACGCAA CTTTAAACGA CAGGCTCTGG
    AGCAAACTTG ACCCCAGCAG CCATCGGGAT AGTGTGTCAT CGTCTTCCAG CATTTCGTCC
    AATGACACTG TGATTGATCT GTCCTTGCCC AACCTTTCCA GGAAAAGCCT TACCTGTCTT
    AGCGCAGCCC GGGATTATTC AGACAAGCAG GCTTCGTTGA CCAACAACCA TCGGTCGGTC
    ATTGCAGGAT GTGGAGTCTC GCTTGTCAGC AAGAGCAAGT CCAACCCCAA CCTGCGGGAT
    GGCGAGATGT GCAATACAGA TGACGATTTG CAACCCAGAC CTCTGGTAGC CGATAAAGAC
    CCCATCCGAC CCATGCAAAG GCGACACACG TGGAGCAGGT TGTACGTTGA AGGCCTAAAG
    CAGTCATCGA CACTTGCTGC TTCTGCAGCC TCCAAGAAGT CAACTACCCT TTCAGCAAAA
    GACATCGACT CAAAGTCCAA AAGTCTGGGA GATCTCACTT CAGACGATAT CATCTGTAAC
    TTCGAGAGCA AGTATCGCAG CATTAGCCGC AGTTTCATCG TGAGGCCAAG TCGCCAACAT
    GGGAAAACTC TGAGGAAACC CCAAAATGAT TTCACTGAGC AGCTGAAGAG GCTCACTGAC
    GTGGAACCCC TTACCAGCAA GGACTTTGCT CAGTGCCGGG CTGACTCAGA AGCAGGACAG
    GAAGAAGAGG AGATGCCTCC TTTACGGAGG AGTTCATCAC GGAGCCAGAG CCGCGTGAGA
    TACATCGCTA ACCGTGCCAG GCAGGCCCAG GAAAGGCAAC GGCTACAAAG CATAACCCGG
    ATGTCTGGGA GCCCCATCGA AGAGCGTGGG AACCCTGAAG GAGCTTGTAG TGTACCCCAT
    AGTCCTTGCA CTTGCCTGGA TCTTTTAAGC CAGCTTCCAC CAGGACCACC CCAACAAAGC
    CCACTGAGCC CGGACAATGA GGTCTTCTTT ATGCTCAGGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa60125
    Clone ID Related Accession (Same CDS sequence) XM_017357540.1 , NM_001347692.1 , XM_017357540.2
    Accession Version XM_017357540.2 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4605bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017357540.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGATACAG CATCCTTGAA CACCTCGCTG TTCTCCGGCA GCATGAGTAC GGATTCTCCC 
    AATTTGTCCA CGAGATCCAT GGGCTTCACT ACTGACACGG ATTCATCCCG CAGCAGCACA
    CTCTTACACT GCAATCATTT CTTGGCAGCC AACTACATCA GTGTGGTGGA AAAATGCATG
    AGTTCAATGC AGACGGGCAC TCAGATGGTA AAACTCCGTG GAGGATCCAA AGGCCTGGTG
    CGCTTTTTCT ACTTGGATGA ACACAAGTCC TGTATCCGCT GGCGACCGTC TAGGAAGAAT
    GAGAAAGCCA AAATCTCGAT CGACTCCATT CGGGAGGTTT GTGAGGGGAA GCAGTCTGAG
    ATCTTCCAGC GGTATTCAGA CGGTAGCTTT GATCCCAACT GCTGCTTCAG CATCTACTAT
    GGCGAGCACA TGGAGTCACT TGACCTGGTG TCTGGCACAG GAGAAGAGGC TCGCACCTGG
    ATTACTGGCC TTAAGTACCT TATGGCCGGC ATCAGCGATG AAGACAGTCT GGCTAAACGA
    CAACGCACTC GTGACCAGTG GCTGAAGCAG ACGTTTGCAG AGGCTGATAA GAATGGAGAC
    GGCTGCCTCA GCATCAGTGA AGTCCTCCAG CTCCTGCACA AGCTCAACGT CAACCTGCCC
    AGGCAGAAGG TCAAGCAGAT GTTTAAGGAA GCAGACACTG ATGACAACCA GGGTACTTTG
    GGATTTGAGG AGTTCTGCTC TTTCTATAAG ATGATGTCAA CGCGGCGTGA TCTGTACCTG
    CTGATGCTCA CCTACAGCAA TCATAAAGAC CACCTGGACA CTGATGACCT CAAGCGCTTC
    CTCGAGACGG AACAAAAGAT GACAAATGTC TCTAAGGAGC ACTGTCTTGA AATCGTGGCC
    AAGTTTGAAC CTTGTCCTGA AAACCAGACA TCGGGTGTGC TGGGGATTGA TGGTTTTACA
    AACTACATGC GCAGCCCTGC GGGGGACATC TTCAACCCTG AACATTACGA GGTGAACCAA
    GACATGACGC AGCCCCTCAG TGATTACTTC ATCGCTTCCT CCCACAACAC GTATCTGATG
    GGAGACCAGC TCATGTCTCA GTCTCGGGTT GATATGTACG CCTGGGTGCT GCAGGCCGGA
    TGCAGATGTG TGGAAGTGGA TTGCTGGGAT GGACAGGATG GAGAACCAAT CGTGCATCAT
    GGCTACACCC TGACTTCCAA AATCCTTTTT AAAGACGTCA TTGAAACCAT CAACAAATAC
    GCATTCGCCA AAAACCAGTA TCCAGTGATT CTGTCCATCG AGAATCACTG CAGCGTCCCT
    CAGCAAAAGA AAATGGCTCA GTATCTGACT GAAGTGTTGG GGGATAAACT GGACGTGTCC
    TCCATCCCCG CTGACCCGTC AGGACTTCTG CCTTCACCTG AAGCCCTGAA AGGCAAAATA
    CTGATCAAGG GCAAGAAACT ACCCTCAAAT ATAGATGAGA ACGCAGAGGA GGGGGACGTG
    TCTGATGAAG ACAGTGCAGA TGAGATGGAG GACGATTGTA AGCTGTTGAA TGGAGATACG
    TCTATGAACC GAAAGCAGGC GGAGAATGTG GCCAAGAAAA AATTAGATAA CCTCATGAAG
    GAGTCAAAGA TTCGCGACCG GGAAGATCCA GACAGTTTTA CCATCACAGC CCTGCCACCG
    TCAGGAAAAC ATGACAAATC GCCACTGAAG GGAAAAGCTG AAGATAGTAC CGACACTGGA
    GATGAGGCCA ACATCGGTGG AAACAAACGT CTGGCCAGAA CCTTCATGGG CAGCTTTTCC
    AAACGCAAGA AAAAGACTAG CAAAGTGAAG AAATCCTCTA GTTTTGAGGA CACCGACACC
    GATCAGGAGA GCTCAAGCGG GGCATCTAAA GCACAAGCAC ATCACAGCAG GAAGAAAAAA
    ACCATGAAGC TGTCAAGAGC CCTTTCTGAC CTGGTATACA TGAAATCTGT GGGAATGCCA
    GACTTTGAGT CTCAAGGTGT CACTTGGCAG GTGTCGTCGA TGAGCGAGAC CAAAGCTCAT
    CAGTTAATCC AGCAGAAGCC GGTGCCGTTC ATGCGGTTCA ACCAGCGGCA GCTCTCTCGC
    GTCTACCCAT CTCCTTACCG CGTGGACTCC AGCAACTTCA ACCCACAGCC CTTCTGGAAC
    GCTGGCTGCC ATCTGGTTGC GCTGAATTAC CAGTCAGAGG GAAGAGTGCT GCAGCTCAAC
    AGAGCTAAGT TCTATTGCAA TGGCAACTGT GGTTACATTC AGAAGCCTGG CTGCTTGTGT
    GAAGGTTCCT TTAATCCAGT AGCTGAGGAT CCTTTGCCAG GTCGATTGAA AAAGCAACTG
    ATTCTTAAGG TCATCAGTGG TCAGCAATTG CCCAAACCAA AAGACTCCAT GCTGGGGGAC
    AGAGGAGAGA TAATCGATCC GTTTGTGGAA GTGGAGATTA TTGGGCTACC GGTGGATTGC
    TGCAAAGAGC AAACCAGAGT GGTTGACGAC AATGGATTCA ATCCTATGTG GGAAGAAACT
    CTCGTCTTCA CTCTGCACAT GCCAGAGAAC GCTCTTATAC GCTTCCTGGT GTGGGATCAT
    GATCCGATAG GCCAAGATTT CATTGGTCAG AGGACCATCG CCTTTAACAG CATGATTCCC
    GGTTACCGCC ATGTGTATTT GGAAGGCATG GAGGAAGCTT CTATCTTTGT TCATGTTGCA
    GTGAATGACA TCACCAGTAA GGCTCGAGCA GTCAGTGGTA TAAAAGGACT CTTTCACAGA
    AACCCAAAGC AAGCATCTCT CGACAGCCAT GCTGCTGCTC TGCTCAACCG TAAGAGTACC
    TTCGGTGCTC ACCTCCTGCG CCGCACAGCC AGTGCCCCCA CCAAGGGCCA ACCCAAAGTC
    AAGAGGGGCT TCCCCGAGAT CTCCATTGAC ACAAAAGATT GCAGCTCGGA CGGCGCCAGC
    GAGGAGCGGG AATCTGAAGA GGGGCCTCTT GTCCACCACA ATGGAGATAC TGCGTCTGCA
    AAGTCATGGG GTCAAGGCAC CAACGGGCAG CTTCACCCTG ATGACAGAAA AGAAAATTCT
    GAAGTGCAGA GTCCAACTCC ATCTCTGAAC GCCTCATGTC TGGAATCCAT CACTGAGAAC
    TTTCAGGAGA ACAGCCCTTC GACCTCAACG CAAAGTCCTG TGGATACCTC ACCGCATTGT
    CCCTTGTCCT CACCATCCAC AGCAATAACG GCATCTTCCA ACGGGGAAAA TCTCAGCCCT
    GTGAAAAATA ACGAGGACAT CCTGAAATCA TTTGCACTAA CCCAATCTAC GAGTGGTAAG
    GATTCTGGGA ACAGTCCTGT AAACTCATCA ATTTCCACTA ATTACCTTGA CATTGGATGC
    GACACGCCAA CTTTTCCCAC TTTCCATCGG GCGGATGGAG AGGTTGCACA AGAGACAGTC
    ATCAATCAAA CAGGACTGAC TCTAGAGATA GATGCTGGGA AGGAACCAAC TGCCATTCAG
    AGCTCTCAGA GGATTCAGGC ATTGAAGGCA GTGTTTGACA GAGACATTGG ACGAAGCATG
    CAAACAGGAC TGAGGACCCC TGTGAGAAGG TCCAAAAGTG AAGGACAAAT GAAAAAGGCA
    GACGGCCCTT CGGTGGTGCC AGAAGTTTGT ACTGACGCAA CTTTAAACGA CAGGCTCTGG
    AGCAAACTTG ACCCCAGCAG CCATCGGGAT AGTGTGTCAT CGTCTTCCAG CATTTCGTCC
    AATGACACTG TGATTGATCT GTCCTTGCCC AACCTTTCCA GGAAAAGCCT TACCTGTCTT
    AGCGCAGCCC GGGATTATTC AGACAAGCAG GCTTCGTTGA CCAACAACCA TCGGTCGGTC
    ATTGCAGGAT GTGGAGTCTC GCTTGTCAGC AAGAGCAAGT CCAACCCCAA CCTGCGGGAT
    GGCGAGATGT GCAATACAGA TGACGATTTG CAACCCAGAC CTCTGGTAGC CGATAAAGAC
    CCCATCCGAC CCATGCAAAG GCGACACACG TGGAGCAGGT TGTACGTTGA AGGCCTAAAG
    CAGTCATCGA CACTTGCTGC TTCTGCAGCC TCCAAGAAGT CAACTACCCT TTCAGCAAAA
    GACATCGACT CAAAGTCCAA AAGTCTGGGA GATCTCACTT CAGACGATAT CATCTGTAAC
    TTCGAGAGCA AGTATCGCAG CATTAGCCGC AGTTTCATCG TGAGGCCAAG TCGCCAACAT
    GGGAAAACTC TGAGGAAACC CCAAAATGAT TTCACTGAGC AGCTGAAGAG GCTCACTGAC
    GTGGAACCCC TTACCAGCAA GGACTTTGCT CAGTGCCGGG CTGACTCAGA AGCAGGACAG
    GAAGAAGAGG AGATGCCTCC TTTACGGAGG AGTTCATCAC GGAGCCAGAG CCGCGTGAGA
    TACATCGCTA ACCGTGCCAG GCAGGCCCAG GAAAGGCAAC GGCTACAAAG CATAACCCGG
    ATGTCTGGGA GCCCCATCGA AGAGCGTGGG AACCCTGAAG GAGCTTGTAG TGTACCCCAT
    AGTCCTTGCA CTTGCCTGGA TCTTTTAAGC CAGCTTCCAC CAGGACCACC CCAACAAAGC
    CCACTGAGCC CGGACAATGA GGTCTTCTTT ATGCTCAGGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_017213029.1
    CDS388..4992
    Translation

    Target ORF information:

    RefSeq Version XM_017357540.2
    Organism Danio rerio(zebrafish)
    Definition Danio rerio phospholipase C, eta 2a (plch2a), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_017357540.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    ATGGATACAG CATCCTTGAA CACCTCGCTG TTCTCCGGCA GCATGAGTAC GGATTCTCCC 
    AATTTGTCCA CGAGATCCAT GGGCTTCACT ACTGACACGG ATTCATCCCG CAGCAGCACA
    CTCTTACACT GCAATCATTT CTTGGCAGCC AACTACATCA GTGTGGTGGA AAAATGCATG
    AGTTCAATGC AGACGGGCAC TCAGATGGTA AAACTCCGTG GAGGATCCAA AGGCCTGGTG
    CGCTTTTTCT ACTTGGATGA ACACAAGTCC TGTATCCGCT GGCGACCGTC TAGGAAGAAT
    GAGAAAGCCA AAATCTCGAT CGACTCCATT CGGGAGGTTT GTGAGGGGAA GCAGTCTGAG
    ATCTTCCAGC GGTATTCAGA CGGTAGCTTT GATCCCAACT GCTGCTTCAG CATCTACTAT
    GGCGAGCACA TGGAGTCACT TGACCTGGTG TCTGGCACAG GAGAAGAGGC TCGCACCTGG
    ATTACTGGCC TTAAGTACCT TATGGCCGGC ATCAGCGATG AAGACAGTCT GGCTAAACGA
    CAACGCACTC GTGACCAGTG GCTGAAGCAG ACGTTTGCAG AGGCTGATAA GAATGGAGAC
    GGCTGCCTCA GCATCAGTGA AGTCCTCCAG CTCCTGCACA AGCTCAACGT CAACCTGCCC
    AGGCAGAAGG TCAAGCAGAT GTTTAAGGAA GCAGACACTG ATGACAACCA GGGTACTTTG
    GGATTTGAGG AGTTCTGCTC TTTCTATAAG ATGATGTCAA CGCGGCGTGA TCTGTACCTG
    CTGATGCTCA CCTACAGCAA TCATAAAGAC CACCTGGACA CTGATGACCT CAAGCGCTTC
    CTCGAGACGG AACAAAAGAT GACAAATGTC TCTAAGGAGC ACTGTCTTGA AATCGTGGCC
    AAGTTTGAAC CTTGTCCTGA AAACCAGACA TCGGGTGTGC TGGGGATTGA TGGTTTTACA
    AACTACATGC GCAGCCCTGC GGGGGACATC TTCAACCCTG AACATTACGA GGTGAACCAA
    GACATGACGC AGCCCCTCAG TGATTACTTC ATCGCTTCCT CCCACAACAC GTATCTGATG
    GGAGACCAGC TCATGTCTCA GTCTCGGGTT GATATGTACG CCTGGGTGCT GCAGGCCGGA
    TGCAGATGTG TGGAAGTGGA TTGCTGGGAT GGACAGGATG GAGAACCAAT CGTGCATCAT
    GGCTACACCC TGACTTCCAA AATCCTTTTT AAAGACGTCA TTGAAACCAT CAACAAATAC
    GCATTCGCCA AAAACCAGTA TCCAGTGATT CTGTCCATCG AGAATCACTG CAGCGTCCCT
    CAGCAAAAGA AAATGGCTCA GTATCTGACT GAAGTGTTGG GGGATAAACT GGACGTGTCC
    TCCATCCCCG CTGACCCGTC AGGACTTCTG CCTTCACCTG AAGCCCTGAA AGGCAAAATA
    CTGATCAAGG GCAAGAAACT ACCCTCAAAT ATAGATGAGA ACGCAGAGGA GGGGGACGTG
    TCTGATGAAG ACAGTGCAGA TGAGATGGAG GACGATTGTA AGCTGTTGAA TGGAGATACG
    TCTATGAACC GAAAGCAGGC GGAGAATGTG GCCAAGAAAA AATTAGATAA CCTCATGAAG
    GAGTCAAAGA TTCGCGACCG GGAAGATCCA GACAGTTTTA CCATCACAGC CCTGCCACCG
    TCAGGAAAAC ATGACAAATC GCCACTGAAG GGAAAAGCTG AAGATAGTAC CGACACTGGA
    GATGAGGCCA ACATCGGTGG AAACAAACGT CTGGCCAGAA CCTTCATGGG CAGCTTTTCC
    AAACGCAAGA AAAAGACTAG CAAAGTGAAG AAATCCTCTA GTTTTGAGGA CACCGACACC
    GATCAGGAGA GCTCAAGCGG GGCATCTAAA GCACAAGCAC ATCACAGCAG GAAGAAAAAA
    ACCATGAAGC TGTCAAGAGC CCTTTCTGAC CTGGTATACA TGAAATCTGT GGGAATGCCA
    GACTTTGAGT CTCAAGGTGT CACTTGGCAG GTGTCGTCGA TGAGCGAGAC CAAAGCTCAT
    CAGTTAATCC AGCAGAAGCC GGTGCCGTTC ATGCGGTTCA ACCAGCGGCA GCTCTCTCGC
    GTCTACCCAT CTCCTTACCG CGTGGACTCC AGCAACTTCA ACCCACAGCC CTTCTGGAAC
    GCTGGCTGCC ATCTGGTTGC GCTGAATTAC CAGTCAGAGG GAAGAGTGCT GCAGCTCAAC
    AGAGCTAAGT TCTATTGCAA TGGCAACTGT GGTTACATTC AGAAGCCTGG CTGCTTGTGT
    GAAGGTTCCT TTAATCCAGT AGCTGAGGAT CCTTTGCCAG GTCGATTGAA AAAGCAACTG
    ATTCTTAAGG TCATCAGTGG TCAGCAATTG CCCAAACCAA AAGACTCCAT GCTGGGGGAC
    AGAGGAGAGA TAATCGATCC GTTTGTGGAA GTGGAGATTA TTGGGCTACC GGTGGATTGC
    TGCAAAGAGC AAACCAGAGT GGTTGACGAC AATGGATTCA ATCCTATGTG GGAAGAAACT
    CTCGTCTTCA CTCTGCACAT GCCAGAGAAC GCTCTTATAC GCTTCCTGGT GTGGGATCAT
    GATCCGATAG GCCAAGATTT CATTGGTCAG AGGACCATCG CCTTTAACAG CATGATTCCC
    GGTTACCGCC ATGTGTATTT GGAAGGCATG GAGGAAGCTT CTATCTTTGT TCATGTTGCA
    GTGAATGACA TCACCAGTAA GGCTCGAGCA GTCAGTGGTA TAAAAGGACT CTTTCACAGA
    AACCCAAAGC AAGCATCTCT CGACAGCCAT GCTGCTGCTC TGCTCAACCG TAAGAGTACC
    TTCGGTGCTC ACCTCCTGCG CCGCACAGCC AGTGCCCCCA CCAAGGGCCA ACCCAAAGTC
    AAGAGGGGCT TCCCCGAGAT CTCCATTGAC ACAAAAGATT GCAGCTCGGA CGGCGCCAGC
    GAGGAGCGGG AATCTGAAGA GGGGCCTCTT GTCCACCACA ATGGAGATAC TGCGTCTGCA
    AAGTCATGGG GTCAAGGCAC CAACGGGCAG CTTCACCCTG ATGACAGAAA AGAAAATTCT
    GAAGTGCAGA GTCCAACTCC ATCTCTGAAC GCCTCATGTC TGGAATCCAT CACTGAGAAC
    TTTCAGGAGA ACAGCCCTTC GACCTCAACG CAAAGTCCTG TGGATACCTC ACCGCATTGT
    CCCTTGTCCT CACCATCCAC AGCAATAACG GCATCTTCCA ACGGGGAAAA TCTCAGCCCT
    GTGAAAAATA ACGAGGACAT CCTGAAATCA TTTGCACTAA CCCAATCTAC GAGTGGTAAG
    GATTCTGGGA ACAGTCCTGT AAACTCATCA ATTTCCACTA ATTACCTTGA CATTGGATGC
    GACACGCCAA CTTTTCCCAC TTTCCATCGG GCGGATGGAG AGGTTGCACA AGAGACAGTC
    ATCAATCAAA CAGGACTGAC TCTAGAGATA GATGCTGGGA AGGAACCAAC TGCCATTCAG
    AGCTCTCAGA GGATTCAGGC ATTGAAGGCA GTGTTTGACA GAGACATTGG ACGAAGCATG
    CAAACAGGAC TGAGGACCCC TGTGAGAAGG TCCAAAAGTG AAGGACAAAT GAAAAAGGCA
    GACGGCCCTT CGGTGGTGCC AGAAGTTTGT ACTGACGCAA CTTTAAACGA CAGGCTCTGG
    AGCAAACTTG ACCCCAGCAG CCATCGGGAT AGTGTGTCAT CGTCTTCCAG CATTTCGTCC
    AATGACACTG TGATTGATCT GTCCTTGCCC AACCTTTCCA GGAAAAGCCT TACCTGTCTT
    AGCGCAGCCC GGGATTATTC AGACAAGCAG GCTTCGTTGA CCAACAACCA TCGGTCGGTC
    ATTGCAGGAT GTGGAGTCTC GCTTGTCAGC AAGAGCAAGT CCAACCCCAA CCTGCGGGAT
    GGCGAGATGT GCAATACAGA TGACGATTTG CAACCCAGAC CTCTGGTAGC CGATAAAGAC
    CCCATCCGAC CCATGCAAAG GCGACACACG TGGAGCAGGT TGTACGTTGA AGGCCTAAAG
    CAGTCATCGA CACTTGCTGC TTCTGCAGCC TCCAAGAAGT CAACTACCCT TTCAGCAAAA
    GACATCGACT CAAAGTCCAA AAGTCTGGGA GATCTCACTT CAGACGATAT CATCTGTAAC
    TTCGAGAGCA AGTATCGCAG CATTAGCCGC AGTTTCATCG TGAGGCCAAG TCGCCAACAT
    GGGAAAACTC TGAGGAAACC CCAAAATGAT TTCACTGAGC AGCTGAAGAG GCTCACTGAC
    GTGGAACCCC TTACCAGCAA GGACTTTGCT CAGTGCCGGG CTGACTCAGA AGCAGGACAG
    GAAGAAGAGG AGATGCCTCC TTTACGGAGG AGTTCATCAC GGAGCCAGAG CCGCGTGAGA
    TACATCGCTA ACCGTGCCAG GCAGGCCCAG GAAAGGCAAC GGCTACAAAG CATAACCCGG
    ATGTCTGGGA GCCCCATCGA AGAGCGTGGG AACCCTGAAG GAGCTTGTAG TGTACCCCAT
    AGTCCTTGCA CTTGCCTGGA TCTTTTAAGC CAGCTTCCAC CAGGACCACC CCAACAAAGC
    CCACTGAGCC CGGACAATGA GGTCTTCTTT ATGCTCAGGC TGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa49930
    Clone ID Related Accession (Same CDS sequence) XM_009304266.2 , XM_009304266.3
    Accession Version XM_009304266.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4674bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304266.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTGTGCCG CTGTCTTGCC TGCCAGCTCG GTCACTTCTG TCGTGATGCC CCCAGCATTA 
    GGGCCAGTAC ATGCTACTAA ATCAGCAACT CCACCGGTCC GAGTACCGAG CCCAGTGGTG
    GCATCAGCCT GCTCGAAGGC ACTGCGTAAG CTGACCCTGT CAAGACTCCT TGCGGGGTTC
    TTTTGGACTG GAGAGAATAC TGTGTATGGA TCCAAATGGA GGCTTGGCTT GTTTGTGGAA
    AAATGCATGA GTTCAATGCA GACGGGCACT CAGATGGTAA AACTCCGTGG AGGATCCAAA
    GGCCTGGTGC GCTTTTTCTA CTTGGATGAA CACAAGTCCT GTATCCGCTG GCGACCGTCT
    AGGAAGAATG AGAAAGCCAA AATCTCGATC GACTCCATTC GGGAGGTTTG TGAGGGGAAG
    CAGTCTGAGA TCTTCCAGCG GTATTCAGAC GGTAGCTTTG ATCCCAACTG CTGCTTCAGC
    ATCTACTATG GCGAGCACAT GGAGTCACTT GACCTGGTGT CTGGCACAGG AGAAGAGGCT
    CGCACCTGGA TTACTGGCCT TAAGTACCTT ATGGCCGGCA TCAGCGATGA AGACAGTCTG
    GCTAAACGAC AACGCACTCG TGACCAGTGG CTGAAGCAGA CGTTTGCAGA GGCTGATAAG
    AATGGAGACG GCTGCCTCAG CATCAGTGAA GTCCTCCAGC TCCTGCACAA GCTCAACGTC
    AACCTGCCCA GGCAGAAGGT CAAGCAGATG TTTAAGGAAG CAGACACTGA TGACAACCAG
    GGTACTTTGG GATTTGAGGA GTTCTGCTCT TTCTATAAGA TGATGTCAAC GCGGCGTGAT
    CTGTACCTGC TGATGCTCAC CTACAGCAAT CATAAAGACC ACCTGGACAC TGATGACCTC
    AAGCGCTTCC TCGAGACGGA ACAAAAGATG ACAAATGTCT CTAAGGAGCA CTGTCTTGAA
    ATCGTGGCCA AGTTTGAACC TTGTCCTGAA AACCAGACAT CGGGTGTGCT GGGGATTGAT
    GGTTTTACAA ACTACATGCG CAGCCCTGCG GGGGACATCT TCAACCCTGA ACATTACGAG
    GTGAACCAAG ACATGACGCA GCCCCTCAGT GATTACTTCA TCGCTTCCTC CCACAACACG
    TATCTGATGG GAGACCAGCT CATGTCTCAG TCTCGGGTTG ATATGTACGC CTGGGTGCTG
    CAGGCCGGAT GCAGATGTGT GGAAGTGGAT TGCTGGGATG GACAGGATGG AGAACCAATC
    GTGCATCATG GCTACACCCT GACTTCCAAA ATCCTTTTTA AAGACGTCAT TGAAACCATC
    AACAAATACG CATTCGCCAA AAACCAGTAT CCAGTGATTC TGTCCATCGA GAATCACTGC
    AGCGTCCCTC AGCAAAAGAA AATGGCTCAG TATCTGACTG AAGTGTTGGG GGATAAACTG
    GACGTGTCCT CCATCCCCGC TGACCCGTCA GGACTTCTGC CTTCACCTGA AGCCCTGAAA
    GGCAAAATAC TGATCAAGGG CAAGAAACTA CCCTCAAATA TAGATGAGAA CGCAGAGGAG
    GGGGACGTGT CTGATGAAGA CAGTGCAGAT GAGATGGAGG ACGATTGTAA GCTGTTGAAT
    GGAGATACGT CTATGAACCG AAAGCAGGCG GAGAATGTGG CCAAGAAAAA ATTAGATAAC
    CTCATGAAGG AGTCAAAGAT TCGCGACCGG GAAGATCCAG ACAGTTTTAC CATCACAGCC
    CTGCCACCGT CAGGAAAACA TGACAAATCG CCACTGAAGG GAAAAGCTGA AGATAGTACC
    GACACTGGAG ATGAGGCCAA CATCGGTGGA AACAAACGTC TGGCCAGAAC CTTCATGGGC
    AGCTTTTCCA AACGCAAGAA AAAGACTAGC AAAGTGAAGA AATCCTCTAG TTTTGAGGAC
    ACCGACACCG ATCAGGAGAG CTCAAGCGGG GCATCTAAAG CACAAGCACA TCACAGCAGG
    AAGAAAAAAA CCATGAAGCT GTCAAGAGCC CTTTCTGACC TGGTATACAT GAAATCTGTG
    GGAATGCCAG ACTTTGAGTC TCAAGGTGTC ACTTGGCAGG TGTCGTCGAT GAGCGAGACC
    AAAGCTCATC AGTTAATCCA GCAGAAGCCG GTGCCGTTCA TGCGGTTCAA CCAGCGGCAG
    CTCTCTCGCG TCTACCCATC TCCTTACCGC GTGGACTCCA GCAACTTCAA CCCACAGCCC
    TTCTGGAACG CTGGCTGCCA TCTGGTTGCG CTGAATTACC AGTCAGAGGG AAGAGTGCTG
    CAGCTCAACA GAGCTAAGTT CTATTGCAAT GGCAACTGTG GTTACATTCA GAAGCCTGGC
    TGCTTGTGTG AAGGTTCCTT TAATCCAGTA GCTGAGGATC CTTTGCCAGG TCGATTGAAA
    AAGCAACTGA TTCTTAAGGT CATCAGTGGT CAGCAATTGC CCAAACCAAA AGACTCCATG
    CTGGGGGACA GAGGAGAGAT AATCGATCCG TTTGTGGAAG TGGAGATTAT TGGGCTACCG
    GTGGATTGCT GCAAAGAGCA AACCAGAGTG GTTGACGACA ATGGATTCAA TCCTATGTGG
    GAAGAAACTC TCGTCTTCAC TCTGCACATG CCAGAGAACG CTCTTATACG CTTCCTGGTG
    TGGGATCATG ATCCGATAGG CCAAGATTTC ATTGGTCAGA GGACCATCGC CTTTAACAGC
    ATGATTCCCG GTTACCGCCA TGTGTATTTG GAAGGCATGG AGGAAGCTTC TATCTTTGTT
    CATGTTGCAG TGAATGACAT CACCAGTAAG GCTCGAGCAG TCAGTGGTAT AAAAGGACTC
    TTTCACAGAA ACCCAAAGCA AGCATCTCTC GACAGCCATG CTGCTGCTCT GCTCAACCGT
    AAGAGTACCT TCGGTGCTCA CCTCCTGCGC CGCACAGCCA GTGCCCCCAC CAAGGGCCAA
    CCCAAAGTCA AGAGGGGCTT CCCCGAGATC TCCATTGACA CAAAAGATTG CAGCTCGGAC
    GGCGCCAGCG AGGAGCGGGA ATCTGAAGAG GGGCCTCTTG TCCACCACAA TGGAGATACT
    GCGTCTGCAA AGTCATGGGG TCAAGGCACC AACGGGCAGC TTCACCCTGA TGACAGAAAA
    GAAAATTCTG AAGTGCAGAG TCCAACTCCA TCTCTGAACG CCTCATGTCT GGAATCCATC
    ACTGAGAACT TTCAGGAGAA CAGCCCTTCG ACCTCAACGC AAAGTCCTGT GGATACCTCA
    CCGCATTGTC CCTTGTCCTC ACCATCCACA GCAATAACGG CATCTTCCAA CGGGGAAAAT
    CTCAGCCCTG TGAAAAATAA CGAGGACATC CTGAAATCAT TTGCACTAAC CCAATCTACG
    AGTGGTAAGG ATTCTGGGAA CAGTCCTGTA AACTCATCAA TTTCCACTAA TTACCTTGAC
    ATTGGATGCG ACACGCCAAC TTTTCCCACT TTCCATCGGG CGGATGGAGA GGTTGCACAA
    GAGACAGTCA TCAATCAAAC AGGACTGACT CTAGAGATAG ATGCTGGGAA GGAACCAACT
    GCCATTCAGA GCTCTCAGAG GATTCAGGCA TTGAAGGCAG TGTTTGACAG AGACATTGGA
    CGAAGCATGC AAACAGGACT GAGGACCCCT GTGAGAAGGT CCAAAAGTGA AGGACAAATG
    AAAAAGGCAG ACGGCCCTTC GGTGGTGCCA GAAGTTTGTA CTGACGCAAC TTTAAACGAC
    AGGCTCTGGA GCAAACTTGA CCCCAGCAGC CATCGGGATA GTGTGTCATC GTCTTCCAGC
    ATTTCGTCCA ATGACACTGT GATTGATCTG TCCTTGCCCA ACCTTTCCAG GAAAAGCCTT
    ACCTGTCTTA GCGCAGCCCG GGATTATTCA GACAAGCAGG CTTCGTTGAC CAACAACCAT
    CGGTCGGTCA TTGCAGGATG TGGAGTCTCG CTTGTCAGCA AGAGCAAGTC CAACCCCAAC
    CTGCGGGATG GCGAGATGTG CAATACAGAT GACGATTTGC AACCCAGACC TCTGGTAGCC
    GATAAAGACC CCATCCGACC CATGCAAAGG CGACACACGT GGAGCAGGTT GTACGTTGAA
    GGCCTAAAGC AGTCATCGAC ACTTGCTGCT TCTGCAGCCT CCAAGAAGTC AACTACCCTT
    TCAGCAAAAG ACATCGACTC AAAGTCCAAA AGTCTGGGAG ATCTCACTTC AGACGATATC
    ATCTGTAACT TCGAGAGCAA GTATCGCAGC ATTAGCCGCA GTTTCATCGT GAGGCCAAGT
    CGCCAACATG GGAAAACTCT GAGGAAACCC CAAAATGATT TCACTGAGCA GCTGAAGAGG
    CTCACTGACG TGGAACCCCT TACCAGCAAG GACTTTGCTC AGTGCCGGGC TGACTCAGAA
    GCAGGACAGG AAGAAGAGGA GATGCCTCCT TTACGGAGGA GTTCATCACG GAGCCAGAGC
    CGCGTGAGAT ACATCGCTAA CCGTGCCAGG CAGGCCCAGG AAAGGCAACG GCTACAAAGC
    ATAACCCGGA TGTCTGGGAG CCCCATCGAA GAGCGTGGGA ACCCTGAAGG AGCTTGTAGT
    GTACCCCATA GTCCTTGCAC TTGCCTGGAT CTTTTAAGCC AGCTTCCACC AGGACCACCC
    CAACAAAGCC CACTGAGCCC GGACAATGAG GTCTTCTTTA TGCTCAGGCT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009302541.1
    CDS263..4936
    Translation

    Target ORF information:

    RefSeq Version XM_009304266.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio phospholipase C eta 2 (plch2), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009304266.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTGTGCCG CTGTCTTGCC TGCCAGCTCG GTCACTTCTG TCGTGATGCC CCCAGCATTA 
    GGGCCAGTAC ATGCTACTAA ATCAGCAACT CCACCGGTCC GAGTACCGAG CCCAGTGGTG
    GCATCAGCCT GCTCGAAGGC ACTGCGTAAG CTGACCCTGT CAAGACTCCT TGCGGGGTTC
    TTTTGGACTG GAGAGAATAC TGTGTATGGA TCCAAATGGA GGCTTGGCTT GTTTGTGGAA
    AAATGCATGA GTTCAATGCA GACGGGCACT CAGATGGTAA AACTCCGTGG AGGATCCAAA
    GGCCTGGTGC GCTTTTTCTA CTTGGATGAA CACAAGTCCT GTATCCGCTG GCGACCGTCT
    AGGAAGAATG AGAAAGCCAA AATCTCGATC GACTCCATTC GGGAGGTTTG TGAGGGGAAG
    CAGTCTGAGA TCTTCCAGCG GTATTCAGAC GGTAGCTTTG ATCCCAACTG CTGCTTCAGC
    ATCTACTATG GCGAGCACAT GGAGTCACTT GACCTGGTGT CTGGCACAGG AGAAGAGGCT
    CGCACCTGGA TTACTGGCCT TAAGTACCTT ATGGCCGGCA TCAGCGATGA AGACAGTCTG
    GCTAAACGAC AACGCACTCG TGACCAGTGG CTGAAGCAGA CGTTTGCAGA GGCTGATAAG
    AATGGAGACG GCTGCCTCAG CATCAGTGAA GTCCTCCAGC TCCTGCACAA GCTCAACGTC
    AACCTGCCCA GGCAGAAGGT CAAGCAGATG TTTAAGGAAG CAGACACTGA TGACAACCAG
    GGTACTTTGG GATTTGAGGA GTTCTGCTCT TTCTATAAGA TGATGTCAAC GCGGCGTGAT
    CTGTACCTGC TGATGCTCAC CTACAGCAAT CATAAAGACC ACCTGGACAC TGATGACCTC
    AAGCGCTTCC TCGAGACGGA ACAAAAGATG ACAAATGTCT CTAAGGAGCA CTGTCTTGAA
    ATCGTGGCCA AGTTTGAACC TTGTCCTGAA AACCAGACAT CGGGTGTGCT GGGGATTGAT
    GGTTTTACAA ACTACATGCG CAGCCCTGCG GGGGACATCT TCAACCCTGA ACATTACGAG
    GTGAACCAAG ACATGACGCA GCCCCTCAGT GATTACTTCA TCGCTTCCTC CCACAACACG
    TATCTGATGG GAGACCAGCT CATGTCTCAG TCTCGGGTTG ATATGTACGC CTGGGTGCTG
    CAGGCCGGAT GCAGATGTGT GGAAGTGGAT TGCTGGGATG GACAGGATGG AGAACCAATC
    GTGCATCATG GCTACACCCT GACTTCCAAA ATCCTTTTTA AAGACGTCAT TGAAACCATC
    AACAAATACG CATTCGCCAA AAACCAGTAT CCAGTGATTC TGTCCATCGA GAATCACTGC
    AGCGTCCCTC AGCAAAAGAA AATGGCTCAG TATCTGACTG AAGTGTTGGG GGATAAACTG
    GACGTGTCCT CCATCCCCGC TGACCCGTCA GGACTTCTGC CTTCACCTGA AGCCCTGAAA
    GGCAAAATAC TGATCAAGGG CAAGAAACTA CCCTCAAATA TAGATGAGAA CGCAGAGGAG
    GGGGACGTGT CTGATGAAGA CAGTGCAGAT GAGATGGAGG ACGATTGTAA GCTGTTGAAT
    GGAGATACGT CTATGAACCG AAAGCAGGCG GAGAATGTGG CCAAGAAAAA ATTAGATAAC
    CTCATGAAGG AGTCAAAGAT TCGCGACCGG GAAGATCCAG ACAGTTTTAC CATCACAGCC
    CTGCCACCGT CAGGAAAACA TGACAAATCG CCACTGAAGG GAAAAGCTGA AGATAGTACC
    GACACTGGAG ATGAGGCCAA CATCGGTGGA AACAAACGTC TGGCCAGAAC CTTCATGGGC
    AGCTTTTCCA AACGCAAGAA AAAGACTAGC AAAGTGAAGA AATCCTCTAG TTTTGAGGAC
    ACCGACACCG ATCAGGAGAG CTCAAGCGGG GCATCTAAAG CACAAGCACA TCACAGCAGG
    AAGAAAAAAA CCATGAAGCT GTCAAGAGCC CTTTCTGACC TGGTATACAT GAAATCTGTG
    GGAATGCCAG ACTTTGAGTC TCAAGGTGTC ACTTGGCAGG TGTCGTCGAT GAGCGAGACC
    AAAGCTCATC AGTTAATCCA GCAGAAGCCG GTGCCGTTCA TGCGGTTCAA CCAGCGGCAG
    CTCTCTCGCG TCTACCCATC TCCTTACCGC GTGGACTCCA GCAACTTCAA CCCACAGCCC
    TTCTGGAACG CTGGCTGCCA TCTGGTTGCG CTGAATTACC AGTCAGAGGG AAGAGTGCTG
    CAGCTCAACA GAGCTAAGTT CTATTGCAAT GGCAACTGTG GTTACATTCA GAAGCCTGGC
    TGCTTGTGTG AAGGTTCCTT TAATCCAGTA GCTGAGGATC CTTTGCCAGG TCGATTGAAA
    AAGCAACTGA TTCTTAAGGT CATCAGTGGT CAGCAATTGC CCAAACCAAA AGACTCCATG
    CTGGGGGACA GAGGAGAGAT AATCGATCCG TTTGTGGAAG TGGAGATTAT TGGGCTACCG
    GTGGATTGCT GCAAAGAGCA AACCAGAGTG GTTGACGACA ATGGATTCAA TCCTATGTGG
    GAAGAAACTC TCGTCTTCAC TCTGCACATG CCAGAGAACG CTCTTATACG CTTCCTGGTG
    TGGGATCATG ATCCGATAGG CCAAGATTTC ATTGGTCAGA GGACCATCGC CTTTAACAGC
    ATGATTCCCG GTTACCGCCA TGTGTATTTG GAAGGCATGG AGGAAGCTTC TATCTTTGTT
    CATGTTGCAG TGAATGACAT CACCAGTAAG GCTCGAGCAG TCAGTGGTAT AAAAGGACTC
    TTTCACAGAA ACCCAAAGCA AGCATCTCTC GACAGCCATG CTGCTGCTCT GCTCAACCGT
    AAGAGTACCT TCGGTGCTCA CCTCCTGCGC CGCACAGCCA GTGCCCCCAC CAAGGGCCAA
    CCCAAAGTCA AGAGGGGCTT CCCCGAGATC TCCATTGACA CAAAAGATTG CAGCTCGGAC
    GGCGCCAGCG AGGAGCGGGA ATCTGAAGAG GGGCCTCTTG TCCACCACAA TGGAGATACT
    GCGTCTGCAA AGTCATGGGG TCAAGGCACC AACGGGCAGC TTCACCCTGA TGACAGAAAA
    GAAAATTCTG AAGTGCAGAG TCCAACTCCA TCTCTGAACG CCTCATGTCT GGAATCCATC
    ACTGAGAACT TTCAGGAGAA CAGCCCTTCG ACCTCAACGC AAAGTCCTGT GGATACCTCA
    CCGCATTGTC CCTTGTCCTC ACCATCCACA GCAATAACGG CATCTTCCAA CGGGGAAAAT
    CTCAGCCCTG TGAAAAATAA CGAGGACATC CTGAAATCAT TTGCACTAAC CCAATCTACG
    AGTGGTAAGG ATTCTGGGAA CAGTCCTGTA AACTCATCAA TTTCCACTAA TTACCTTGAC
    ATTGGATGCG ACACGCCAAC TTTTCCCACT TTCCATCGGG CGGATGGAGA GGTTGCACAA
    GAGACAGTCA TCAATCAAAC AGGACTGACT CTAGAGATAG ATGCTGGGAA GGAACCAACT
    GCCATTCAGA GCTCTCAGAG GATTCAGGCA TTGAAGGCAG TGTTTGACAG AGACATTGGA
    CGAAGCATGC AAACAGGACT GAGGACCCCT GTGAGAAGGT CCAAAAGTGA AGGACAAATG
    AAAAAGGCAG ACGGCCCTTC GGTGGTGCCA GAAGTTTGTA CTGACGCAAC TTTAAACGAC
    AGGCTCTGGA GCAAACTTGA CCCCAGCAGC CATCGGGATA GTGTGTCATC GTCTTCCAGC
    ATTTCGTCCA ATGACACTGT GATTGATCTG TCCTTGCCCA ACCTTTCCAG GAAAAGCCTT
    ACCTGTCTTA GCGCAGCCCG GGATTATTCA GACAAGCAGG CTTCGTTGAC CAACAACCAT
    CGGTCGGTCA TTGCAGGATG TGGAGTCTCG CTTGTCAGCA AGAGCAAGTC CAACCCCAAC
    CTGCGGGATG GCGAGATGTG CAATACAGAT GACGATTTGC AACCCAGACC TCTGGTAGCC
    GATAAAGACC CCATCCGACC CATGCAAAGG CGACACACGT GGAGCAGGTT GTACGTTGAA
    GGCCTAAAGC AGTCATCGAC ACTTGCTGCT TCTGCAGCCT CCAAGAAGTC AACTACCCTT
    TCAGCAAAAG ACATCGACTC AAAGTCCAAA AGTCTGGGAG ATCTCACTTC AGACGATATC
    ATCTGTAACT TCGAGAGCAA GTATCGCAGC ATTAGCCGCA GTTTCATCGT GAGGCCAAGT
    CGCCAACATG GGAAAACTCT GAGGAAACCC CAAAATGATT TCACTGAGCA GCTGAAGAGG
    CTCACTGACG TGGAACCCCT TACCAGCAAG GACTTTGCTC AGTGCCGGGC TGACTCAGAA
    GCAGGACAGG AAGAAGAGGA GATGCCTCCT TTACGGAGGA GTTCATCACG GAGCCAGAGC
    CGCGTGAGAT ACATCGCTAA CCGTGCCAGG CAGGCCCAGG AAAGGCAACG GCTACAAAGC
    ATAACCCGGA TGTCTGGGAG CCCCATCGAA GAGCGTGGGA ACCCTGAAGG AGCTTGTAGT
    GTACCCCATA GTCCTTGCAC TTGCCTGGAT CTTTTAAGCC AGCTTCCACC AGGACCACCC
    CAACAAAGCC CACTGAGCCC GGACAATGAG GTCTTCTTTA TGCTCAGGCT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa49930
    Clone ID Related Accession (Same CDS sequence) XM_009304266.2 , XM_009304266.3
    Accession Version XM_009304266.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4674bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304266.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTGTGCCG CTGTCTTGCC TGCCAGCTCG GTCACTTCTG TCGTGATGCC CCCAGCATTA 
    GGGCCAGTAC ATGCTACTAA ATCAGCAACT CCACCGGTCC GAGTACCGAG CCCAGTGGTG
    GCATCAGCCT GCTCGAAGGC ACTGCGTAAG CTGACCCTGT CAAGACTCCT TGCGGGGTTC
    TTTTGGACTG GAGAGAATAC TGTGTATGGA TCCAAATGGA GGCTTGGCTT GTTTGTGGAA
    AAATGCATGA GTTCAATGCA GACGGGCACT CAGATGGTAA AACTCCGTGG AGGATCCAAA
    GGCCTGGTGC GCTTTTTCTA CTTGGATGAA CACAAGTCCT GTATCCGCTG GCGACCGTCT
    AGGAAGAATG AGAAAGCCAA AATCTCGATC GACTCCATTC GGGAGGTTTG TGAGGGGAAG
    CAGTCTGAGA TCTTCCAGCG GTATTCAGAC GGTAGCTTTG ATCCCAACTG CTGCTTCAGC
    ATCTACTATG GCGAGCACAT GGAGTCACTT GACCTGGTGT CTGGCACAGG AGAAGAGGCT
    CGCACCTGGA TTACTGGCCT TAAGTACCTT ATGGCCGGCA TCAGCGATGA AGACAGTCTG
    GCTAAACGAC AACGCACTCG TGACCAGTGG CTGAAGCAGA CGTTTGCAGA GGCTGATAAG
    AATGGAGACG GCTGCCTCAG CATCAGTGAA GTCCTCCAGC TCCTGCACAA GCTCAACGTC
    AACCTGCCCA GGCAGAAGGT CAAGCAGATG TTTAAGGAAG CAGACACTGA TGACAACCAG
    GGTACTTTGG GATTTGAGGA GTTCTGCTCT TTCTATAAGA TGATGTCAAC GCGGCGTGAT
    CTGTACCTGC TGATGCTCAC CTACAGCAAT CATAAAGACC ACCTGGACAC TGATGACCTC
    AAGCGCTTCC TCGAGACGGA ACAAAAGATG ACAAATGTCT CTAAGGAGCA CTGTCTTGAA
    ATCGTGGCCA AGTTTGAACC TTGTCCTGAA AACCAGACAT CGGGTGTGCT GGGGATTGAT
    GGTTTTACAA ACTACATGCG CAGCCCTGCG GGGGACATCT TCAACCCTGA ACATTACGAG
    GTGAACCAAG ACATGACGCA GCCCCTCAGT GATTACTTCA TCGCTTCCTC CCACAACACG
    TATCTGATGG GAGACCAGCT CATGTCTCAG TCTCGGGTTG ATATGTACGC CTGGGTGCTG
    CAGGCCGGAT GCAGATGTGT GGAAGTGGAT TGCTGGGATG GACAGGATGG AGAACCAATC
    GTGCATCATG GCTACACCCT GACTTCCAAA ATCCTTTTTA AAGACGTCAT TGAAACCATC
    AACAAATACG CATTCGCCAA AAACCAGTAT CCAGTGATTC TGTCCATCGA GAATCACTGC
    AGCGTCCCTC AGCAAAAGAA AATGGCTCAG TATCTGACTG AAGTGTTGGG GGATAAACTG
    GACGTGTCCT CCATCCCCGC TGACCCGTCA GGACTTCTGC CTTCACCTGA AGCCCTGAAA
    GGCAAAATAC TGATCAAGGG CAAGAAACTA CCCTCAAATA TAGATGAGAA CGCAGAGGAG
    GGGGACGTGT CTGATGAAGA CAGTGCAGAT GAGATGGAGG ACGATTGTAA GCTGTTGAAT
    GGAGATACGT CTATGAACCG AAAGCAGGCG GAGAATGTGG CCAAGAAAAA ATTAGATAAC
    CTCATGAAGG AGTCAAAGAT TCGCGACCGG GAAGATCCAG ACAGTTTTAC CATCACAGCC
    CTGCCACCGT CAGGAAAACA TGACAAATCG CCACTGAAGG GAAAAGCTGA AGATAGTACC
    GACACTGGAG ATGAGGCCAA CATCGGTGGA AACAAACGTC TGGCCAGAAC CTTCATGGGC
    AGCTTTTCCA AACGCAAGAA AAAGACTAGC AAAGTGAAGA AATCCTCTAG TTTTGAGGAC
    ACCGACACCG ATCAGGAGAG CTCAAGCGGG GCATCTAAAG CACAAGCACA TCACAGCAGG
    AAGAAAAAAA CCATGAAGCT GTCAAGAGCC CTTTCTGACC TGGTATACAT GAAATCTGTG
    GGAATGCCAG ACTTTGAGTC TCAAGGTGTC ACTTGGCAGG TGTCGTCGAT GAGCGAGACC
    AAAGCTCATC AGTTAATCCA GCAGAAGCCG GTGCCGTTCA TGCGGTTCAA CCAGCGGCAG
    CTCTCTCGCG TCTACCCATC TCCTTACCGC GTGGACTCCA GCAACTTCAA CCCACAGCCC
    TTCTGGAACG CTGGCTGCCA TCTGGTTGCG CTGAATTACC AGTCAGAGGG AAGAGTGCTG
    CAGCTCAACA GAGCTAAGTT CTATTGCAAT GGCAACTGTG GTTACATTCA GAAGCCTGGC
    TGCTTGTGTG AAGGTTCCTT TAATCCAGTA GCTGAGGATC CTTTGCCAGG TCGATTGAAA
    AAGCAACTGA TTCTTAAGGT CATCAGTGGT CAGCAATTGC CCAAACCAAA AGACTCCATG
    CTGGGGGACA GAGGAGAGAT AATCGATCCG TTTGTGGAAG TGGAGATTAT TGGGCTACCG
    GTGGATTGCT GCAAAGAGCA AACCAGAGTG GTTGACGACA ATGGATTCAA TCCTATGTGG
    GAAGAAACTC TCGTCTTCAC TCTGCACATG CCAGAGAACG CTCTTATACG CTTCCTGGTG
    TGGGATCATG ATCCGATAGG CCAAGATTTC ATTGGTCAGA GGACCATCGC CTTTAACAGC
    ATGATTCCCG GTTACCGCCA TGTGTATTTG GAAGGCATGG AGGAAGCTTC TATCTTTGTT
    CATGTTGCAG TGAATGACAT CACCAGTAAG GCTCGAGCAG TCAGTGGTAT AAAAGGACTC
    TTTCACAGAA ACCCAAAGCA AGCATCTCTC GACAGCCATG CTGCTGCTCT GCTCAACCGT
    AAGAGTACCT TCGGTGCTCA CCTCCTGCGC CGCACAGCCA GTGCCCCCAC CAAGGGCCAA
    CCCAAAGTCA AGAGGGGCTT CCCCGAGATC TCCATTGACA CAAAAGATTG CAGCTCGGAC
    GGCGCCAGCG AGGAGCGGGA ATCTGAAGAG GGGCCTCTTG TCCACCACAA TGGAGATACT
    GCGTCTGCAA AGTCATGGGG TCAAGGCACC AACGGGCAGC TTCACCCTGA TGACAGAAAA
    GAAAATTCTG AAGTGCAGAG TCCAACTCCA TCTCTGAACG CCTCATGTCT GGAATCCATC
    ACTGAGAACT TTCAGGAGAA CAGCCCTTCG ACCTCAACGC AAAGTCCTGT GGATACCTCA
    CCGCATTGTC CCTTGTCCTC ACCATCCACA GCAATAACGG CATCTTCCAA CGGGGAAAAT
    CTCAGCCCTG TGAAAAATAA CGAGGACATC CTGAAATCAT TTGCACTAAC CCAATCTACG
    AGTGGTAAGG ATTCTGGGAA CAGTCCTGTA AACTCATCAA TTTCCACTAA TTACCTTGAC
    ATTGGATGCG ACACGCCAAC TTTTCCCACT TTCCATCGGG CGGATGGAGA GGTTGCACAA
    GAGACAGTCA TCAATCAAAC AGGACTGACT CTAGAGATAG ATGCTGGGAA GGAACCAACT
    GCCATTCAGA GCTCTCAGAG GATTCAGGCA TTGAAGGCAG TGTTTGACAG AGACATTGGA
    CGAAGCATGC AAACAGGACT GAGGACCCCT GTGAGAAGGT CCAAAAGTGA AGGACAAATG
    AAAAAGGCAG ACGGCCCTTC GGTGGTGCCA GAAGTTTGTA CTGACGCAAC TTTAAACGAC
    AGGCTCTGGA GCAAACTTGA CCCCAGCAGC CATCGGGATA GTGTGTCATC GTCTTCCAGC
    ATTTCGTCCA ATGACACTGT GATTGATCTG TCCTTGCCCA ACCTTTCCAG GAAAAGCCTT
    ACCTGTCTTA GCGCAGCCCG GGATTATTCA GACAAGCAGG CTTCGTTGAC CAACAACCAT
    CGGTCGGTCA TTGCAGGATG TGGAGTCTCG CTTGTCAGCA AGAGCAAGTC CAACCCCAAC
    CTGCGGGATG GCGAGATGTG CAATACAGAT GACGATTTGC AACCCAGACC TCTGGTAGCC
    GATAAAGACC CCATCCGACC CATGCAAAGG CGACACACGT GGAGCAGGTT GTACGTTGAA
    GGCCTAAAGC AGTCATCGAC ACTTGCTGCT TCTGCAGCCT CCAAGAAGTC AACTACCCTT
    TCAGCAAAAG ACATCGACTC AAAGTCCAAA AGTCTGGGAG ATCTCACTTC AGACGATATC
    ATCTGTAACT TCGAGAGCAA GTATCGCAGC ATTAGCCGCA GTTTCATCGT GAGGCCAAGT
    CGCCAACATG GGAAAACTCT GAGGAAACCC CAAAATGATT TCACTGAGCA GCTGAAGAGG
    CTCACTGACG TGGAACCCCT TACCAGCAAG GACTTTGCTC AGTGCCGGGC TGACTCAGAA
    GCAGGACAGG AAGAAGAGGA GATGCCTCCT TTACGGAGGA GTTCATCACG GAGCCAGAGC
    CGCGTGAGAT ACATCGCTAA CCGTGCCAGG CAGGCCCAGG AAAGGCAACG GCTACAAAGC
    ATAACCCGGA TGTCTGGGAG CCCCATCGAA GAGCGTGGGA ACCCTGAAGG AGCTTGTAGT
    GTACCCCATA GTCCTTGCAC TTGCCTGGAT CTTTTAAGCC AGCTTCCACC AGGACCACCC
    CAACAAAGCC CACTGAGCCC GGACAATGAG GTCTTCTTTA TGCTCAGGCT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009302541.1
    CDS266..4939
    Translation

    Target ORF information:

    RefSeq Version XM_009304266.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio phospholipase C, eta 2a (plch2a), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009304266.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    4501
    4561
    4621
    ATGTGTGCCG CTGTCTTGCC TGCCAGCTCG GTCACTTCTG TCGTGATGCC CCCAGCATTA 
    GGGCCAGTAC ATGCTACTAA ATCAGCAACT CCACCGGTCC GAGTACCGAG CCCAGTGGTG
    GCATCAGCCT GCTCGAAGGC ACTGCGTAAG CTGACCCTGT CAAGACTCCT TGCGGGGTTC
    TTTTGGACTG GAGAGAATAC TGTGTATGGA TCCAAATGGA GGCTTGGCTT GTTTGTGGAA
    AAATGCATGA GTTCAATGCA GACGGGCACT CAGATGGTAA AACTCCGTGG AGGATCCAAA
    GGCCTGGTGC GCTTTTTCTA CTTGGATGAA CACAAGTCCT GTATCCGCTG GCGACCGTCT
    AGGAAGAATG AGAAAGCCAA AATCTCGATC GACTCCATTC GGGAGGTTTG TGAGGGGAAG
    CAGTCTGAGA TCTTCCAGCG GTATTCAGAC GGTAGCTTTG ATCCCAACTG CTGCTTCAGC
    ATCTACTATG GCGAGCACAT GGAGTCACTT GACCTGGTGT CTGGCACAGG AGAAGAGGCT
    CGCACCTGGA TTACTGGCCT TAAGTACCTT ATGGCCGGCA TCAGCGATGA AGACAGTCTG
    GCTAAACGAC AACGCACTCG TGACCAGTGG CTGAAGCAGA CGTTTGCAGA GGCTGATAAG
    AATGGAGACG GCTGCCTCAG CATCAGTGAA GTCCTCCAGC TCCTGCACAA GCTCAACGTC
    AACCTGCCCA GGCAGAAGGT CAAGCAGATG TTTAAGGAAG CAGACACTGA TGACAACCAG
    GGTACTTTGG GATTTGAGGA GTTCTGCTCT TTCTATAAGA TGATGTCAAC GCGGCGTGAT
    CTGTACCTGC TGATGCTCAC CTACAGCAAT CATAAAGACC ACCTGGACAC TGATGACCTC
    AAGCGCTTCC TCGAGACGGA ACAAAAGATG ACAAATGTCT CTAAGGAGCA CTGTCTTGAA
    ATCGTGGCCA AGTTTGAACC TTGTCCTGAA AACCAGACAT CGGGTGTGCT GGGGATTGAT
    GGTTTTACAA ACTACATGCG CAGCCCTGCG GGGGACATCT TCAACCCTGA ACATTACGAG
    GTGAACCAAG ACATGACGCA GCCCCTCAGT GATTACTTCA TCGCTTCCTC CCACAACACG
    TATCTGATGG GAGACCAGCT CATGTCTCAG TCTCGGGTTG ATATGTACGC CTGGGTGCTG
    CAGGCCGGAT GCAGATGTGT GGAAGTGGAT TGCTGGGATG GACAGGATGG AGAACCAATC
    GTGCATCATG GCTACACCCT GACTTCCAAA ATCCTTTTTA AAGACGTCAT TGAAACCATC
    AACAAATACG CATTCGCCAA AAACCAGTAT CCAGTGATTC TGTCCATCGA GAATCACTGC
    AGCGTCCCTC AGCAAAAGAA AATGGCTCAG TATCTGACTG AAGTGTTGGG GGATAAACTG
    GACGTGTCCT CCATCCCCGC TGACCCGTCA GGACTTCTGC CTTCACCTGA AGCCCTGAAA
    GGCAAAATAC TGATCAAGGG CAAGAAACTA CCCTCAAATA TAGATGAGAA CGCAGAGGAG
    GGGGACGTGT CTGATGAAGA CAGTGCAGAT GAGATGGAGG ACGATTGTAA GCTGTTGAAT
    GGAGATACGT CTATGAACCG AAAGCAGGCG GAGAATGTGG CCAAGAAAAA ATTAGATAAC
    CTCATGAAGG AGTCAAAGAT TCGCGACCGG GAAGATCCAG ACAGTTTTAC CATCACAGCC
    CTGCCACCGT CAGGAAAACA TGACAAATCG CCACTGAAGG GAAAAGCTGA AGATAGTACC
    GACACTGGAG ATGAGGCCAA CATCGGTGGA AACAAACGTC TGGCCAGAAC CTTCATGGGC
    AGCTTTTCCA AACGCAAGAA AAAGACTAGC AAAGTGAAGA AATCCTCTAG TTTTGAGGAC
    ACCGACACCG ATCAGGAGAG CTCAAGCGGG GCATCTAAAG CACAAGCACA TCACAGCAGG
    AAGAAAAAAA CCATGAAGCT GTCAAGAGCC CTTTCTGACC TGGTATACAT GAAATCTGTG
    GGAATGCCAG ACTTTGAGTC TCAAGGTGTC ACTTGGCAGG TGTCGTCGAT GAGCGAGACC
    AAAGCTCATC AGTTAATCCA GCAGAAGCCG GTGCCGTTCA TGCGGTTCAA CCAGCGGCAG
    CTCTCTCGCG TCTACCCATC TCCTTACCGC GTGGACTCCA GCAACTTCAA CCCACAGCCC
    TTCTGGAACG CTGGCTGCCA TCTGGTTGCG CTGAATTACC AGTCAGAGGG AAGAGTGCTG
    CAGCTCAACA GAGCTAAGTT CTATTGCAAT GGCAACTGTG GTTACATTCA GAAGCCTGGC
    TGCTTGTGTG AAGGTTCCTT TAATCCAGTA GCTGAGGATC CTTTGCCAGG TCGATTGAAA
    AAGCAACTGA TTCTTAAGGT CATCAGTGGT CAGCAATTGC CCAAACCAAA AGACTCCATG
    CTGGGGGACA GAGGAGAGAT AATCGATCCG TTTGTGGAAG TGGAGATTAT TGGGCTACCG
    GTGGATTGCT GCAAAGAGCA AACCAGAGTG GTTGACGACA ATGGATTCAA TCCTATGTGG
    GAAGAAACTC TCGTCTTCAC TCTGCACATG CCAGAGAACG CTCTTATACG CTTCCTGGTG
    TGGGATCATG ATCCGATAGG CCAAGATTTC ATTGGTCAGA GGACCATCGC CTTTAACAGC
    ATGATTCCCG GTTACCGCCA TGTGTATTTG GAAGGCATGG AGGAAGCTTC TATCTTTGTT
    CATGTTGCAG TGAATGACAT CACCAGTAAG GCTCGAGCAG TCAGTGGTAT AAAAGGACTC
    TTTCACAGAA ACCCAAAGCA AGCATCTCTC GACAGCCATG CTGCTGCTCT GCTCAACCGT
    AAGAGTACCT TCGGTGCTCA CCTCCTGCGC CGCACAGCCA GTGCCCCCAC CAAGGGCCAA
    CCCAAAGTCA AGAGGGGCTT CCCCGAGATC TCCATTGACA CAAAAGATTG CAGCTCGGAC
    GGCGCCAGCG AGGAGCGGGA ATCTGAAGAG GGGCCTCTTG TCCACCACAA TGGAGATACT
    GCGTCTGCAA AGTCATGGGG TCAAGGCACC AACGGGCAGC TTCACCCTGA TGACAGAAAA
    GAAAATTCTG AAGTGCAGAG TCCAACTCCA TCTCTGAACG CCTCATGTCT GGAATCCATC
    ACTGAGAACT TTCAGGAGAA CAGCCCTTCG ACCTCAACGC AAAGTCCTGT GGATACCTCA
    CCGCATTGTC CCTTGTCCTC ACCATCCACA GCAATAACGG CATCTTCCAA CGGGGAAAAT
    CTCAGCCCTG TGAAAAATAA CGAGGACATC CTGAAATCAT TTGCACTAAC CCAATCTACG
    AGTGGTAAGG ATTCTGGGAA CAGTCCTGTA AACTCATCAA TTTCCACTAA TTACCTTGAC
    ATTGGATGCG ACACGCCAAC TTTTCCCACT TTCCATCGGG CGGATGGAGA GGTTGCACAA
    GAGACAGTCA TCAATCAAAC AGGACTGACT CTAGAGATAG ATGCTGGGAA GGAACCAACT
    GCCATTCAGA GCTCTCAGAG GATTCAGGCA TTGAAGGCAG TGTTTGACAG AGACATTGGA
    CGAAGCATGC AAACAGGACT GAGGACCCCT GTGAGAAGGT CCAAAAGTGA AGGACAAATG
    AAAAAGGCAG ACGGCCCTTC GGTGGTGCCA GAAGTTTGTA CTGACGCAAC TTTAAACGAC
    AGGCTCTGGA GCAAACTTGA CCCCAGCAGC CATCGGGATA GTGTGTCATC GTCTTCCAGC
    ATTTCGTCCA ATGACACTGT GATTGATCTG TCCTTGCCCA ACCTTTCCAG GAAAAGCCTT
    ACCTGTCTTA GCGCAGCCCG GGATTATTCA GACAAGCAGG CTTCGTTGAC CAACAACCAT
    CGGTCGGTCA TTGCAGGATG TGGAGTCTCG CTTGTCAGCA AGAGCAAGTC CAACCCCAAC
    CTGCGGGATG GCGAGATGTG CAATACAGAT GACGATTTGC AACCCAGACC TCTGGTAGCC
    GATAAAGACC CCATCCGACC CATGCAAAGG CGACACACGT GGAGCAGGTT GTACGTTGAA
    GGCCTAAAGC AGTCATCGAC ACTTGCTGCT TCTGCAGCCT CCAAGAAGTC AACTACCCTT
    TCAGCAAAAG ACATCGACTC AAAGTCCAAA AGTCTGGGAG ATCTCACTTC AGACGATATC
    ATCTGTAACT TCGAGAGCAA GTATCGCAGC ATTAGCCGCA GTTTCATCGT GAGGCCAAGT
    CGCCAACATG GGAAAACTCT GAGGAAACCC CAAAATGATT TCACTGAGCA GCTGAAGAGG
    CTCACTGACG TGGAACCCCT TACCAGCAAG GACTTTGCTC AGTGCCGGGC TGACTCAGAA
    GCAGGACAGG AAGAAGAGGA GATGCCTCCT TTACGGAGGA GTTCATCACG GAGCCAGAGC
    CGCGTGAGAT ACATCGCTAA CCGTGCCAGG CAGGCCCAGG AAAGGCAACG GCTACAAAGC
    ATAACCCGGA TGTCTGGGAG CCCCATCGAA GAGCGTGGGA ACCCTGAAGG AGCTTGTAGT
    GTACCCCATA GTCCTTGCAC TTGCCTGGAT CTTTTAAGCC AGCTTCCACC AGGACCACCC
    CAACAAAGCC CACTGAGCCC GGACAATGAG GTCTTCTTTA TGCTCAGGCT GTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa49931
    Clone ID Related Accession (Same CDS sequence) XM_009304267.2 , XM_009304267.3
    Accession Version XM_009304267.2 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4482bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304267.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAGGATT TTAGCAATAT TAGGAGTCTT AGTCCTGAGA AAGTGGAAAA ATGCATGAGT 
    TCAATGCAGA CGGGCACTCA GATGGTAAAA CTCCGTGGAG GATCCAAAGG CCTGGTGCGC
    TTTTTCTACT TGGATGAACA CAAGTCCTGT ATCCGCTGGC GACCGTCTAG GAAGAATGAG
    AAAGCCAAAA TCTCGATCGA CTCCATTCGG GAGGTTTGTG AGGGGAAGCA GTCTGAGATC
    TTCCAGCGGT ATTCAGACGG TAGCTTTGAT CCCAACTGCT GCTTCAGCAT CTACTATGGC
    GAGCACATGG AGTCACTTGA CCTGGTGTCT GGCACAGGAG AAGAGGCTCG CACCTGGATT
    ACTGGCCTTA AGTACCTTAT GGCCGGCATC AGCGATGAAG ACAGTCTGGC TAAACGACAA
    CGCACTCGTG ACCAGTGGCT GAAGCAGACG TTTGCAGAGG CTGATAAGAA TGGAGACGGC
    TGCCTCAGCA TCAGTGAAGT CCTCCAGCTC CTGCACAAGC TCAACGTCAA CCTGCCCAGG
    CAGAAGGTCA AGCAGATGTT TAAGGAAGCA GACACTGATG ACAACCAGGG TACTTTGGGA
    TTTGAGGAGT TCTGCTCTTT CTATAAGATG ATGTCAACGC GGCGTGATCT GTACCTGCTG
    ATGCTCACCT ACAGCAATCA TAAAGACCAC CTGGACACTG ATGACCTCAA GCGCTTCCTC
    GAGACGGAAC AAAAGATGAC AAATGTCTCT AAGGAGCACT GTCTTGAAAT CGTGGCCAAG
    TTTGAACCTT GTCCTGAAAA CCAGACATCG GGTGTGCTGG GGATTGATGG TTTTACAAAC
    TACATGCGCA GCCCTGCGGG GGACATCTTC AACCCTGAAC ATTACGAGGT GAACCAAGAC
    ATGACGCAGC CCCTCAGTGA TTACTTCATC GCTTCCTCCC ACAACACGTA TCTGATGGGA
    GACCAGCTCA TGTCTCAGTC TCGGGTTGAT ATGTACGCCT GGGTGCTGCA GGCCGGATGC
    AGATGTGTGG AAGTGGATTG CTGGGATGGA CAGGATGGAG AACCAATCGT GCATCATGGC
    TACACCCTGA CTTCCAAAAT CCTTTTTAAA GACGTCATTG AAACCATCAA CAAATACGCA
    TTCGCCAAAA ACCAGTATCC AGTGATTCTG TCCATCGAGA ATCACTGCAG CGTCCCTCAG
    CAAAAGAAAA TGGCTCAGTA TCTGACTGAA GTGTTGGGGG ATAAACTGGA CGTGTCCTCC
    ATCCCCGCTG ACCCGTCAGG ACTTCTGCCT TCACCTGAAG CCCTGAAAGG CAAAATACTG
    ATCAAGGGCA AGAAACTACC CTCAAATATA GATGAGAACG CAGAGGAGGG GGACGTGTCT
    GATGAAGACA GTGCAGATGA GATGGAGGAC GATTGTAAGC TGTTGAATGG AGATACGTCT
    ATGAACCGAA AGCAGGCGGA GAATGTGGCC AAGAAAAAAT TAGATAACCT CATGAAGGAG
    TCAAAGATTC GCGACCGGGA AGATCCAGAC AGTTTTACCA TCACAGCCCT GCCACCGTCA
    GGAAAACATG ACAAATCGCC ACTGAAGGGA AAAGCTGAAG ATAGTACCGA CACTGGAGAT
    GAGGCCAACA TCGGTGGAAA CAAACGTCTG GCCAGAACCT TCATGGGCAG CTTTTCCAAA
    CGCAAGAAAA AGACTAGCAA AGTGAAGAAA TCCTCTAGTT TTGAGGACAC CGACACCGAT
    CAGGAGAGCT CAAGCGGGGC ATCTAAAGCA CAAGCACATC ACAGCAGGAA GAAAAAAACC
    ATGAAGCTGT CAAGAGCCCT TTCTGACCTG GTATACATGA AATCTGTGGG AATGCCAGAC
    TTTGAGTCTC AAGGTGTCAC TTGGCAGGTG TCGTCGATGA GCGAGACCAA AGCTCATCAG
    TTAATCCAGC AGAAGCCGGT GCCGTTCATG CGGTTCAACC AGCGGCAGCT CTCTCGCGTC
    TACCCATCTC CTTACCGCGT GGACTCCAGC AACTTCAACC CACAGCCCTT CTGGAACGCT
    GGCTGCCATC TGGTTGCGCT GAATTACCAG TCAGAGGGAA GAGTGCTGCA GCTCAACAGA
    GCTAAGTTCT ATTGCAATGG CAACTGTGGT TACATTCAGA AGCCTGGCTG CTTGTGTGAA
    GGTTCCTTTA ATCCAGTAGC TGAGGATCCT TTGCCAGGTC GATTGAAAAA GCAACTGATT
    CTTAAGGTCA TCAGTGGTCA GCAATTGCCC AAACCAAAAG ACTCCATGCT GGGGGACAGA
    GGAGAGATAA TCGATCCGTT TGTGGAAGTG GAGATTATTG GGCTACCGGT GGATTGCTGC
    AAAGAGCAAA CCAGAGTGGT TGACGACAAT GGATTCAATC CTATGTGGGA AGAAACTCTC
    GTCTTCACTC TGCACATGCC AGAGAACGCT CTTATACGCT TCCTGGTGTG GGATCATGAT
    CCGATAGGCC AAGATTTCAT TGGTCAGAGG ACCATCGCCT TTAACAGCAT GATTCCCGGT
    TACCGCCATG TGTATTTGGA AGGCATGGAG GAAGCTTCTA TCTTTGTTCA TGTTGCAGTG
    AATGACATCA CCAGTAAGGC TCGAGCAGTC AGTGGTATAA AAGGACTCTT TCACAGAAAC
    CCAAAGCAAG CATCTCTCGA CAGCCATGCT GCTGCTCTGC TCAACCGTAA GAGTACCTTC
    GGTGCTCACC TCCTGCGCCG CACAGCCAGT GCCCCCACCA AGGGCCAACC CAAAGTCAAG
    AGGGGCTTCC CCGAGATCTC CATTGACACA AAAGATTGCA GCTCGGACGG CGCCAGCGAG
    GAGCGGGAAT CTGAAGAGGG GCCTCTTGTC CACCACAATG GAGATACTGC GTCTGCAAAG
    TCATGGGGTC AAGGCACCAA CGGGCAGCTT CACCCTGATG ACAGAAAAGA AAATTCTGAA
    GTGCAGAGTC CAACTCCATC TCTGAACGCC TCATGTCTGG AATCCATCAC TGAGAACTTT
    CAGGAGAACA GCCCTTCGAC CTCAACGCAA AGTCCTGTGG ATACCTCACC GCATTGTCCC
    TTGTCCTCAC CATCCACAGC AATAACGGCA TCTTCCAACG GGGAAAATCT CAGCCCTGTG
    AAAAATAACG AGGACATCCT GAAATCATTT GCACTAACCC AATCTACGAG TGGTAAGGAT
    TCTGGGAACA GTCCTGTAAA CTCATCAATT TCCACTAATT ACCTTGACAT TGGATGCGAC
    ACGCCAACTT TTCCCACTTT CCATCGGGCG GATGGAGAGG TTGCACAAGA GACAGTCATC
    AATCAAACAG GACTGACTCT AGAGATAGAT GCTGGGAAGG AACCAACTGC CATTCAGAGC
    TCTCAGAGGA TTCAGGCATT GAAGGCAGTG TTTGACAGAG ACATTGGACG AAGCATGCAA
    ACAGGACTGA GGACCCCTGT GAGAAGGTCC AAAAGTGAAG GACAAATGAA AAAGGCAGAC
    GGCCCTTCGG TGGTGCCAGA AGTTTGTACT GACGCAACTT TAAACGACAG GCTCTGGAGC
    AAACTTGACC CCAGCAGCCA TCGGGATAGT GTGTCATCGT CTTCCAGCAT TTCGTCCAAT
    GACACTGTGA TTGATCTGTC CTTGCCCAAC CTTTCCAGGA AAAGCCTTAC CTGTCTTAGC
    GCAGCCCGGG ATTATTCAGA CAAGCAGGCT TCGTTGACCA ACAACCATCG GTCGGTCATT
    GCAGGATGTG GAGTCTCGCT TGTCAGCAAG AGCAAGTCCA ACCCCAACCT GCGGGATGGC
    GAGATGTGCA ATACAGATGA CGATTTGCAA CCCAGACCTC TGGTAGCCGA TAAAGACCCC
    ATCCGACCCA TGCAAAGGCG ACACACGTGG AGCAGGTTGT ACGTTGAAGG CCTAAAGCAG
    TCATCGACAC TTGCTGCTTC TGCAGCCTCC AAGAAGTCAA CTACCCTTTC AGCAAAAGAC
    ATCGACTCAA AGTCCAAAAG TCTGGGAGAT CTCACTTCAG ACGATATCAT CTGTAACTTC
    GAGAGCAAGT ATCGCAGCAT TAGCCGCAGT TTCATCGTGA GGCCAAGTCG CCAACATGGG
    AAAACTCTGA GGAAACCCCA AAATGATTTC ACTGAGCAGC TGAAGAGGCT CACTGACGTG
    GAACCCCTTA CCAGCAAGGA CTTTGCTCAG TGCCGGGCTG ACTCAGAAGC AGGACAGGAA
    GAAGAGGAGA TGCCTCCTTT ACGGAGGAGT TCATCACGGA GCCAGAGCCG CGTGAGATAC
    ATCGCTAACC GTGCCAGGCA GGCCCAGGAA AGGCAACGGC TACAAAGCAT AACCCGGATG
    TCTGGGAGCC CCATCGAAGA GCGTGGGAAC CCTGAAGGAG CTTGTAGTGT ACCCCATAGT
    CCTTGCACTT GCCTGGATCT TTTAAGCCAG CTTCCACCAG GACCACCCCA ACAAAGCCCA
    CTGAGCCCGG ACAATGAGGT CTTCTTTATG CTCAGGCTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009302542.1
    CDS583..5064
    Translation

    Target ORF information:

    RefSeq Version XM_009304267.2
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio phospholipase C eta 2 (plch2), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009304267.2

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAGGATT TTAGCAATAT TAGGAGTCTT AGTCCTGAGA AAGTGGAAAA ATGCATGAGT 
    TCAATGCAGA CGGGCACTCA GATGGTAAAA CTCCGTGGAG GATCCAAAGG CCTGGTGCGC
    TTTTTCTACT TGGATGAACA CAAGTCCTGT ATCCGCTGGC GACCGTCTAG GAAGAATGAG
    AAAGCCAAAA TCTCGATCGA CTCCATTCGG GAGGTTTGTG AGGGGAAGCA GTCTGAGATC
    TTCCAGCGGT ATTCAGACGG TAGCTTTGAT CCCAACTGCT GCTTCAGCAT CTACTATGGC
    GAGCACATGG AGTCACTTGA CCTGGTGTCT GGCACAGGAG AAGAGGCTCG CACCTGGATT
    ACTGGCCTTA AGTACCTTAT GGCCGGCATC AGCGATGAAG ACAGTCTGGC TAAACGACAA
    CGCACTCGTG ACCAGTGGCT GAAGCAGACG TTTGCAGAGG CTGATAAGAA TGGAGACGGC
    TGCCTCAGCA TCAGTGAAGT CCTCCAGCTC CTGCACAAGC TCAACGTCAA CCTGCCCAGG
    CAGAAGGTCA AGCAGATGTT TAAGGAAGCA GACACTGATG ACAACCAGGG TACTTTGGGA
    TTTGAGGAGT TCTGCTCTTT CTATAAGATG ATGTCAACGC GGCGTGATCT GTACCTGCTG
    ATGCTCACCT ACAGCAATCA TAAAGACCAC CTGGACACTG ATGACCTCAA GCGCTTCCTC
    GAGACGGAAC AAAAGATGAC AAATGTCTCT AAGGAGCACT GTCTTGAAAT CGTGGCCAAG
    TTTGAACCTT GTCCTGAAAA CCAGACATCG GGTGTGCTGG GGATTGATGG TTTTACAAAC
    TACATGCGCA GCCCTGCGGG GGACATCTTC AACCCTGAAC ATTACGAGGT GAACCAAGAC
    ATGACGCAGC CCCTCAGTGA TTACTTCATC GCTTCCTCCC ACAACACGTA TCTGATGGGA
    GACCAGCTCA TGTCTCAGTC TCGGGTTGAT ATGTACGCCT GGGTGCTGCA GGCCGGATGC
    AGATGTGTGG AAGTGGATTG CTGGGATGGA CAGGATGGAG AACCAATCGT GCATCATGGC
    TACACCCTGA CTTCCAAAAT CCTTTTTAAA GACGTCATTG AAACCATCAA CAAATACGCA
    TTCGCCAAAA ACCAGTATCC AGTGATTCTG TCCATCGAGA ATCACTGCAG CGTCCCTCAG
    CAAAAGAAAA TGGCTCAGTA TCTGACTGAA GTGTTGGGGG ATAAACTGGA CGTGTCCTCC
    ATCCCCGCTG ACCCGTCAGG ACTTCTGCCT TCACCTGAAG CCCTGAAAGG CAAAATACTG
    ATCAAGGGCA AGAAACTACC CTCAAATATA GATGAGAACG CAGAGGAGGG GGACGTGTCT
    GATGAAGACA GTGCAGATGA GATGGAGGAC GATTGTAAGC TGTTGAATGG AGATACGTCT
    ATGAACCGAA AGCAGGCGGA GAATGTGGCC AAGAAAAAAT TAGATAACCT CATGAAGGAG
    TCAAAGATTC GCGACCGGGA AGATCCAGAC AGTTTTACCA TCACAGCCCT GCCACCGTCA
    GGAAAACATG ACAAATCGCC ACTGAAGGGA AAAGCTGAAG ATAGTACCGA CACTGGAGAT
    GAGGCCAACA TCGGTGGAAA CAAACGTCTG GCCAGAACCT TCATGGGCAG CTTTTCCAAA
    CGCAAGAAAA AGACTAGCAA AGTGAAGAAA TCCTCTAGTT TTGAGGACAC CGACACCGAT
    CAGGAGAGCT CAAGCGGGGC ATCTAAAGCA CAAGCACATC ACAGCAGGAA GAAAAAAACC
    ATGAAGCTGT CAAGAGCCCT TTCTGACCTG GTATACATGA AATCTGTGGG AATGCCAGAC
    TTTGAGTCTC AAGGTGTCAC TTGGCAGGTG TCGTCGATGA GCGAGACCAA AGCTCATCAG
    TTAATCCAGC AGAAGCCGGT GCCGTTCATG CGGTTCAACC AGCGGCAGCT CTCTCGCGTC
    TACCCATCTC CTTACCGCGT GGACTCCAGC AACTTCAACC CACAGCCCTT CTGGAACGCT
    GGCTGCCATC TGGTTGCGCT GAATTACCAG TCAGAGGGAA GAGTGCTGCA GCTCAACAGA
    GCTAAGTTCT ATTGCAATGG CAACTGTGGT TACATTCAGA AGCCTGGCTG CTTGTGTGAA
    GGTTCCTTTA ATCCAGTAGC TGAGGATCCT TTGCCAGGTC GATTGAAAAA GCAACTGATT
    CTTAAGGTCA TCAGTGGTCA GCAATTGCCC AAACCAAAAG ACTCCATGCT GGGGGACAGA
    GGAGAGATAA TCGATCCGTT TGTGGAAGTG GAGATTATTG GGCTACCGGT GGATTGCTGC
    AAAGAGCAAA CCAGAGTGGT TGACGACAAT GGATTCAATC CTATGTGGGA AGAAACTCTC
    GTCTTCACTC TGCACATGCC AGAGAACGCT CTTATACGCT TCCTGGTGTG GGATCATGAT
    CCGATAGGCC AAGATTTCAT TGGTCAGAGG ACCATCGCCT TTAACAGCAT GATTCCCGGT
    TACCGCCATG TGTATTTGGA AGGCATGGAG GAAGCTTCTA TCTTTGTTCA TGTTGCAGTG
    AATGACATCA CCAGTAAGGC TCGAGCAGTC AGTGGTATAA AAGGACTCTT TCACAGAAAC
    CCAAAGCAAG CATCTCTCGA CAGCCATGCT GCTGCTCTGC TCAACCGTAA GAGTACCTTC
    GGTGCTCACC TCCTGCGCCG CACAGCCAGT GCCCCCACCA AGGGCCAACC CAAAGTCAAG
    AGGGGCTTCC CCGAGATCTC CATTGACACA AAAGATTGCA GCTCGGACGG CGCCAGCGAG
    GAGCGGGAAT CTGAAGAGGG GCCTCTTGTC CACCACAATG GAGATACTGC GTCTGCAAAG
    TCATGGGGTC AAGGCACCAA CGGGCAGCTT CACCCTGATG ACAGAAAAGA AAATTCTGAA
    GTGCAGAGTC CAACTCCATC TCTGAACGCC TCATGTCTGG AATCCATCAC TGAGAACTTT
    CAGGAGAACA GCCCTTCGAC CTCAACGCAA AGTCCTGTGG ATACCTCACC GCATTGTCCC
    TTGTCCTCAC CATCCACAGC AATAACGGCA TCTTCCAACG GGGAAAATCT CAGCCCTGTG
    AAAAATAACG AGGACATCCT GAAATCATTT GCACTAACCC AATCTACGAG TGGTAAGGAT
    TCTGGGAACA GTCCTGTAAA CTCATCAATT TCCACTAATT ACCTTGACAT TGGATGCGAC
    ACGCCAACTT TTCCCACTTT CCATCGGGCG GATGGAGAGG TTGCACAAGA GACAGTCATC
    AATCAAACAG GACTGACTCT AGAGATAGAT GCTGGGAAGG AACCAACTGC CATTCAGAGC
    TCTCAGAGGA TTCAGGCATT GAAGGCAGTG TTTGACAGAG ACATTGGACG AAGCATGCAA
    ACAGGACTGA GGACCCCTGT GAGAAGGTCC AAAAGTGAAG GACAAATGAA AAAGGCAGAC
    GGCCCTTCGG TGGTGCCAGA AGTTTGTACT GACGCAACTT TAAACGACAG GCTCTGGAGC
    AAACTTGACC CCAGCAGCCA TCGGGATAGT GTGTCATCGT CTTCCAGCAT TTCGTCCAAT
    GACACTGTGA TTGATCTGTC CTTGCCCAAC CTTTCCAGGA AAAGCCTTAC CTGTCTTAGC
    GCAGCCCGGG ATTATTCAGA CAAGCAGGCT TCGTTGACCA ACAACCATCG GTCGGTCATT
    GCAGGATGTG GAGTCTCGCT TGTCAGCAAG AGCAAGTCCA ACCCCAACCT GCGGGATGGC
    GAGATGTGCA ATACAGATGA CGATTTGCAA CCCAGACCTC TGGTAGCCGA TAAAGACCCC
    ATCCGACCCA TGCAAAGGCG ACACACGTGG AGCAGGTTGT ACGTTGAAGG CCTAAAGCAG
    TCATCGACAC TTGCTGCTTC TGCAGCCTCC AAGAAGTCAA CTACCCTTTC AGCAAAAGAC
    ATCGACTCAA AGTCCAAAAG TCTGGGAGAT CTCACTTCAG ACGATATCAT CTGTAACTTC
    GAGAGCAAGT ATCGCAGCAT TAGCCGCAGT TTCATCGTGA GGCCAAGTCG CCAACATGGG
    AAAACTCTGA GGAAACCCCA AAATGATTTC ACTGAGCAGC TGAAGAGGCT CACTGACGTG
    GAACCCCTTA CCAGCAAGGA CTTTGCTCAG TGCCGGGCTG ACTCAGAAGC AGGACAGGAA
    GAAGAGGAGA TGCCTCCTTT ACGGAGGAGT TCATCACGGA GCCAGAGCCG CGTGAGATAC
    ATCGCTAACC GTGCCAGGCA GGCCCAGGAA AGGCAACGGC TACAAAGCAT AACCCGGATG
    TCTGGGAGCC CCATCGAAGA GCGTGGGAAC CCTGAAGGAG CTTGTAGTGT ACCCCATAGT
    CCTTGCACTT GCCTGGATCT TTTAAGCCAG CTTCCACCAG GACCACCCCA ACAAAGCCCA
    CTGAGCCCGG ACAATGAGGT CTTCTTTATG CTCAGGCTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa49931
    Clone ID Related Accession (Same CDS sequence) XM_009304267.2 , XM_009304267.3
    Accession Version XM_009304267.3 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 4482bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product 1-phosphatidylinositol 4,5-bisphosphate phosphodiesterase eta-2 isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007119.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304267.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAGGATT TTAGCAATAT TAGGAGTCTT AGTCCTGAGA AAGTGGAAAA ATGCATGAGT 
    TCAATGCAGA CGGGCACTCA GATGGTAAAA CTCCGTGGAG GATCCAAAGG CCTGGTGCGC
    TTTTTCTACT TGGATGAACA CAAGTCCTGT ATCCGCTGGC GACCGTCTAG GAAGAATGAG
    AAAGCCAAAA TCTCGATCGA CTCCATTCGG GAGGTTTGTG AGGGGAAGCA GTCTGAGATC
    TTCCAGCGGT ATTCAGACGG TAGCTTTGAT CCCAACTGCT GCTTCAGCAT CTACTATGGC
    GAGCACATGG AGTCACTTGA CCTGGTGTCT GGCACAGGAG AAGAGGCTCG CACCTGGATT
    ACTGGCCTTA AGTACCTTAT GGCCGGCATC AGCGATGAAG ACAGTCTGGC TAAACGACAA
    CGCACTCGTG ACCAGTGGCT GAAGCAGACG TTTGCAGAGG CTGATAAGAA TGGAGACGGC
    TGCCTCAGCA TCAGTGAAGT CCTCCAGCTC CTGCACAAGC TCAACGTCAA CCTGCCCAGG
    CAGAAGGTCA AGCAGATGTT TAAGGAAGCA GACACTGATG ACAACCAGGG TACTTTGGGA
    TTTGAGGAGT TCTGCTCTTT CTATAAGATG ATGTCAACGC GGCGTGATCT GTACCTGCTG
    ATGCTCACCT ACAGCAATCA TAAAGACCAC CTGGACACTG ATGACCTCAA GCGCTTCCTC
    GAGACGGAAC AAAAGATGAC AAATGTCTCT AAGGAGCACT GTCTTGAAAT CGTGGCCAAG
    TTTGAACCTT GTCCTGAAAA CCAGACATCG GGTGTGCTGG GGATTGATGG TTTTACAAAC
    TACATGCGCA GCCCTGCGGG GGACATCTTC AACCCTGAAC ATTACGAGGT GAACCAAGAC
    ATGACGCAGC CCCTCAGTGA TTACTTCATC GCTTCCTCCC ACAACACGTA TCTGATGGGA
    GACCAGCTCA TGTCTCAGTC TCGGGTTGAT ATGTACGCCT GGGTGCTGCA GGCCGGATGC
    AGATGTGTGG AAGTGGATTG CTGGGATGGA CAGGATGGAG AACCAATCGT GCATCATGGC
    TACACCCTGA CTTCCAAAAT CCTTTTTAAA GACGTCATTG AAACCATCAA CAAATACGCA
    TTCGCCAAAA ACCAGTATCC AGTGATTCTG TCCATCGAGA ATCACTGCAG CGTCCCTCAG
    CAAAAGAAAA TGGCTCAGTA TCTGACTGAA GTGTTGGGGG ATAAACTGGA CGTGTCCTCC
    ATCCCCGCTG ACCCGTCAGG ACTTCTGCCT TCACCTGAAG CCCTGAAAGG CAAAATACTG
    ATCAAGGGCA AGAAACTACC CTCAAATATA GATGAGAACG CAGAGGAGGG GGACGTGTCT
    GATGAAGACA GTGCAGATGA GATGGAGGAC GATTGTAAGC TGTTGAATGG AGATACGTCT
    ATGAACCGAA AGCAGGCGGA GAATGTGGCC AAGAAAAAAT TAGATAACCT CATGAAGGAG
    TCAAAGATTC GCGACCGGGA AGATCCAGAC AGTTTTACCA TCACAGCCCT GCCACCGTCA
    GGAAAACATG ACAAATCGCC ACTGAAGGGA AAAGCTGAAG ATAGTACCGA CACTGGAGAT
    GAGGCCAACA TCGGTGGAAA CAAACGTCTG GCCAGAACCT TCATGGGCAG CTTTTCCAAA
    CGCAAGAAAA AGACTAGCAA AGTGAAGAAA TCCTCTAGTT TTGAGGACAC CGACACCGAT
    CAGGAGAGCT CAAGCGGGGC ATCTAAAGCA CAAGCACATC ACAGCAGGAA GAAAAAAACC
    ATGAAGCTGT CAAGAGCCCT TTCTGACCTG GTATACATGA AATCTGTGGG AATGCCAGAC
    TTTGAGTCTC AAGGTGTCAC TTGGCAGGTG TCGTCGATGA GCGAGACCAA AGCTCATCAG
    TTAATCCAGC AGAAGCCGGT GCCGTTCATG CGGTTCAACC AGCGGCAGCT CTCTCGCGTC
    TACCCATCTC CTTACCGCGT GGACTCCAGC AACTTCAACC CACAGCCCTT CTGGAACGCT
    GGCTGCCATC TGGTTGCGCT GAATTACCAG TCAGAGGGAA GAGTGCTGCA GCTCAACAGA
    GCTAAGTTCT ATTGCAATGG CAACTGTGGT TACATTCAGA AGCCTGGCTG CTTGTGTGAA
    GGTTCCTTTA ATCCAGTAGC TGAGGATCCT TTGCCAGGTC GATTGAAAAA GCAACTGATT
    CTTAAGGTCA TCAGTGGTCA GCAATTGCCC AAACCAAAAG ACTCCATGCT GGGGGACAGA
    GGAGAGATAA TCGATCCGTT TGTGGAAGTG GAGATTATTG GGCTACCGGT GGATTGCTGC
    AAAGAGCAAA CCAGAGTGGT TGACGACAAT GGATTCAATC CTATGTGGGA AGAAACTCTC
    GTCTTCACTC TGCACATGCC AGAGAACGCT CTTATACGCT TCCTGGTGTG GGATCATGAT
    CCGATAGGCC AAGATTTCAT TGGTCAGAGG ACCATCGCCT TTAACAGCAT GATTCCCGGT
    TACCGCCATG TGTATTTGGA AGGCATGGAG GAAGCTTCTA TCTTTGTTCA TGTTGCAGTG
    AATGACATCA CCAGTAAGGC TCGAGCAGTC AGTGGTATAA AAGGACTCTT TCACAGAAAC
    CCAAAGCAAG CATCTCTCGA CAGCCATGCT GCTGCTCTGC TCAACCGTAA GAGTACCTTC
    GGTGCTCACC TCCTGCGCCG CACAGCCAGT GCCCCCACCA AGGGCCAACC CAAAGTCAAG
    AGGGGCTTCC CCGAGATCTC CATTGACACA AAAGATTGCA GCTCGGACGG CGCCAGCGAG
    GAGCGGGAAT CTGAAGAGGG GCCTCTTGTC CACCACAATG GAGATACTGC GTCTGCAAAG
    TCATGGGGTC AAGGCACCAA CGGGCAGCTT CACCCTGATG ACAGAAAAGA AAATTCTGAA
    GTGCAGAGTC CAACTCCATC TCTGAACGCC TCATGTCTGG AATCCATCAC TGAGAACTTT
    CAGGAGAACA GCCCTTCGAC CTCAACGCAA AGTCCTGTGG ATACCTCACC GCATTGTCCC
    TTGTCCTCAC CATCCACAGC AATAACGGCA TCTTCCAACG GGGAAAATCT CAGCCCTGTG
    AAAAATAACG AGGACATCCT GAAATCATTT GCACTAACCC AATCTACGAG TGGTAAGGAT
    TCTGGGAACA GTCCTGTAAA CTCATCAATT TCCACTAATT ACCTTGACAT TGGATGCGAC
    ACGCCAACTT TTCCCACTTT CCATCGGGCG GATGGAGAGG TTGCACAAGA GACAGTCATC
    AATCAAACAG GACTGACTCT AGAGATAGAT GCTGGGAAGG AACCAACTGC CATTCAGAGC
    TCTCAGAGGA TTCAGGCATT GAAGGCAGTG TTTGACAGAG ACATTGGACG AAGCATGCAA
    ACAGGACTGA GGACCCCTGT GAGAAGGTCC AAAAGTGAAG GACAAATGAA AAAGGCAGAC
    GGCCCTTCGG TGGTGCCAGA AGTTTGTACT GACGCAACTT TAAACGACAG GCTCTGGAGC
    AAACTTGACC CCAGCAGCCA TCGGGATAGT GTGTCATCGT CTTCCAGCAT TTCGTCCAAT
    GACACTGTGA TTGATCTGTC CTTGCCCAAC CTTTCCAGGA AAAGCCTTAC CTGTCTTAGC
    GCAGCCCGGG ATTATTCAGA CAAGCAGGCT TCGTTGACCA ACAACCATCG GTCGGTCATT
    GCAGGATGTG GAGTCTCGCT TGTCAGCAAG AGCAAGTCCA ACCCCAACCT GCGGGATGGC
    GAGATGTGCA ATACAGATGA CGATTTGCAA CCCAGACCTC TGGTAGCCGA TAAAGACCCC
    ATCCGACCCA TGCAAAGGCG ACACACGTGG AGCAGGTTGT ACGTTGAAGG CCTAAAGCAG
    TCATCGACAC TTGCTGCTTC TGCAGCCTCC AAGAAGTCAA CTACCCTTTC AGCAAAAGAC
    ATCGACTCAA AGTCCAAAAG TCTGGGAGAT CTCACTTCAG ACGATATCAT CTGTAACTTC
    GAGAGCAAGT ATCGCAGCAT TAGCCGCAGT TTCATCGTGA GGCCAAGTCG CCAACATGGG
    AAAACTCTGA GGAAACCCCA AAATGATTTC ACTGAGCAGC TGAAGAGGCT CACTGACGTG
    GAACCCCTTA CCAGCAAGGA CTTTGCTCAG TGCCGGGCTG ACTCAGAAGC AGGACAGGAA
    GAAGAGGAGA TGCCTCCTTT ACGGAGGAGT TCATCACGGA GCCAGAGCCG CGTGAGATAC
    ATCGCTAACC GTGCCAGGCA GGCCCAGGAA AGGCAACGGC TACAAAGCAT AACCCGGATG
    TCTGGGAGCC CCATCGAAGA GCGTGGGAAC CCTGAAGGAG CTTGTAGTGT ACCCCATAGT
    CCTTGCACTT GCCTGGATCT TTTAAGCCAG CTTCCACCAG GACCACCCCA ACAAAGCCCA
    CTGAGCCCGG ACAATGAGGT CTTCTTTATG CTCAGGCTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_009302542.1
    CDS471..4952
    Translation

    Target ORF information:

    RefSeq Version XM_009304267.3
    Organism Danio rerio(zebrafish)
    Definition Danio rerio phospholipase C, eta 2a (plch2a), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_009304267.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    3961
    4021
    4081
    4141
    4201
    4261
    4321
    4381
    4441
    ATGGAGGATT TTAGCAATAT TAGGAGTCTT AGTCCTGAGA AAGTGGAAAA ATGCATGAGT 
    TCAATGCAGA CGGGCACTCA GATGGTAAAA CTCCGTGGAG GATCCAAAGG CCTGGTGCGC
    TTTTTCTACT TGGATGAACA CAAGTCCTGT ATCCGCTGGC GACCGTCTAG GAAGAATGAG
    AAAGCCAAAA TCTCGATCGA CTCCATTCGG GAGGTTTGTG AGGGGAAGCA GTCTGAGATC
    TTCCAGCGGT ATTCAGACGG TAGCTTTGAT CCCAACTGCT GCTTCAGCAT CTACTATGGC
    GAGCACATGG AGTCACTTGA CCTGGTGTCT GGCACAGGAG AAGAGGCTCG CACCTGGATT
    ACTGGCCTTA AGTACCTTAT GGCCGGCATC AGCGATGAAG ACAGTCTGGC TAAACGACAA
    CGCACTCGTG ACCAGTGGCT GAAGCAGACG TTTGCAGAGG CTGATAAGAA TGGAGACGGC
    TGCCTCAGCA TCAGTGAAGT CCTCCAGCTC CTGCACAAGC TCAACGTCAA CCTGCCCAGG
    CAGAAGGTCA AGCAGATGTT TAAGGAAGCA GACACTGATG ACAACCAGGG TACTTTGGGA
    TTTGAGGAGT TCTGCTCTTT CTATAAGATG ATGTCAACGC GGCGTGATCT GTACCTGCTG
    ATGCTCACCT ACAGCAATCA TAAAGACCAC CTGGACACTG ATGACCTCAA GCGCTTCCTC
    GAGACGGAAC AAAAGATGAC AAATGTCTCT AAGGAGCACT GTCTTGAAAT CGTGGCCAAG
    TTTGAACCTT GTCCTGAAAA CCAGACATCG GGTGTGCTGG GGATTGATGG TTTTACAAAC
    TACATGCGCA GCCCTGCGGG GGACATCTTC AACCCTGAAC ATTACGAGGT GAACCAAGAC
    ATGACGCAGC CCCTCAGTGA TTACTTCATC GCTTCCTCCC ACAACACGTA TCTGATGGGA
    GACCAGCTCA TGTCTCAGTC TCGGGTTGAT ATGTACGCCT GGGTGCTGCA GGCCGGATGC
    AGATGTGTGG AAGTGGATTG CTGGGATGGA CAGGATGGAG AACCAATCGT GCATCATGGC
    TACACCCTGA CTTCCAAAAT CCTTTTTAAA GACGTCATTG AAACCATCAA CAAATACGCA
    TTCGCCAAAA ACCAGTATCC AGTGATTCTG TCCATCGAGA ATCACTGCAG CGTCCCTCAG
    CAAAAGAAAA TGGCTCAGTA TCTGACTGAA GTGTTGGGGG ATAAACTGGA CGTGTCCTCC
    ATCCCCGCTG ACCCGTCAGG ACTTCTGCCT TCACCTGAAG CCCTGAAAGG CAAAATACTG
    ATCAAGGGCA AGAAACTACC CTCAAATATA GATGAGAACG CAGAGGAGGG GGACGTGTCT
    GATGAAGACA GTGCAGATGA GATGGAGGAC GATTGTAAGC TGTTGAATGG AGATACGTCT
    ATGAACCGAA AGCAGGCGGA GAATGTGGCC AAGAAAAAAT TAGATAACCT CATGAAGGAG
    TCAAAGATTC GCGACCGGGA AGATCCAGAC AGTTTTACCA TCACAGCCCT GCCACCGTCA
    GGAAAACATG ACAAATCGCC ACTGAAGGGA AAAGCTGAAG ATAGTACCGA CACTGGAGAT
    GAGGCCAACA TCGGTGGAAA CAAACGTCTG GCCAGAACCT TCATGGGCAG CTTTTCCAAA
    CGCAAGAAAA AGACTAGCAA AGTGAAGAAA TCCTCTAGTT TTGAGGACAC CGACACCGAT
    CAGGAGAGCT CAAGCGGGGC ATCTAAAGCA CAAGCACATC ACAGCAGGAA GAAAAAAACC
    ATGAAGCTGT CAAGAGCCCT TTCTGACCTG GTATACATGA AATCTGTGGG AATGCCAGAC
    TTTGAGTCTC AAGGTGTCAC TTGGCAGGTG TCGTCGATGA GCGAGACCAA AGCTCATCAG
    TTAATCCAGC AGAAGCCGGT GCCGTTCATG CGGTTCAACC AGCGGCAGCT CTCTCGCGTC
    TACCCATCTC CTTACCGCGT GGACTCCAGC AACTTCAACC CACAGCCCTT CTGGAACGCT
    GGCTGCCATC TGGTTGCGCT GAATTACCAG TCAGAGGGAA GAGTGCTGCA GCTCAACAGA
    GCTAAGTTCT ATTGCAATGG CAACTGTGGT TACATTCAGA AGCCTGGCTG CTTGTGTGAA
    GGTTCCTTTA ATCCAGTAGC TGAGGATCCT TTGCCAGGTC GATTGAAAAA GCAACTGATT
    CTTAAGGTCA TCAGTGGTCA GCAATTGCCC AAACCAAAAG ACTCCATGCT GGGGGACAGA
    GGAGAGATAA TCGATCCGTT TGTGGAAGTG GAGATTATTG GGCTACCGGT GGATTGCTGC
    AAAGAGCAAA CCAGAGTGGT TGACGACAAT GGATTCAATC CTATGTGGGA AGAAACTCTC
    GTCTTCACTC TGCACATGCC AGAGAACGCT CTTATACGCT TCCTGGTGTG GGATCATGAT
    CCGATAGGCC AAGATTTCAT TGGTCAGAGG ACCATCGCCT TTAACAGCAT GATTCCCGGT
    TACCGCCATG TGTATTTGGA AGGCATGGAG GAAGCTTCTA TCTTTGTTCA TGTTGCAGTG
    AATGACATCA CCAGTAAGGC TCGAGCAGTC AGTGGTATAA AAGGACTCTT TCACAGAAAC
    CCAAAGCAAG CATCTCTCGA CAGCCATGCT GCTGCTCTGC TCAACCGTAA GAGTACCTTC
    GGTGCTCACC TCCTGCGCCG CACAGCCAGT GCCCCCACCA AGGGCCAACC CAAAGTCAAG
    AGGGGCTTCC CCGAGATCTC CATTGACACA AAAGATTGCA GCTCGGACGG CGCCAGCGAG
    GAGCGGGAAT CTGAAGAGGG GCCTCTTGTC CACCACAATG GAGATACTGC GTCTGCAAAG
    TCATGGGGTC AAGGCACCAA CGGGCAGCTT CACCCTGATG ACAGAAAAGA AAATTCTGAA
    GTGCAGAGTC CAACTCCATC TCTGAACGCC TCATGTCTGG AATCCATCAC TGAGAACTTT
    CAGGAGAACA GCCCTTCGAC CTCAACGCAA AGTCCTGTGG ATACCTCACC GCATTGTCCC
    TTGTCCTCAC CATCCACAGC AATAACGGCA TCTTCCAACG GGGAAAATCT CAGCCCTGTG
    AAAAATAACG AGGACATCCT GAAATCATTT GCACTAACCC AATCTACGAG TGGTAAGGAT
    TCTGGGAACA GTCCTGTAAA CTCATCAATT TCCACTAATT ACCTTGACAT TGGATGCGAC
    ACGCCAACTT TTCCCACTTT CCATCGGGCG GATGGAGAGG TTGCACAAGA GACAGTCATC
    AATCAAACAG GACTGACTCT AGAGATAGAT GCTGGGAAGG AACCAACTGC CATTCAGAGC
    TCTCAGAGGA TTCAGGCATT GAAGGCAGTG TTTGACAGAG ACATTGGACG AAGCATGCAA
    ACAGGACTGA GGACCCCTGT GAGAAGGTCC AAAAGTGAAG GACAAATGAA AAAGGCAGAC
    GGCCCTTCGG TGGTGCCAGA AGTTTGTACT GACGCAACTT TAAACGACAG GCTCTGGAGC
    AAACTTGACC CCAGCAGCCA TCGGGATAGT GTGTCATCGT CTTCCAGCAT TTCGTCCAAT
    GACACTGTGA TTGATCTGTC CTTGCCCAAC CTTTCCAGGA AAAGCCTTAC CTGTCTTAGC
    GCAGCCCGGG ATTATTCAGA CAAGCAGGCT TCGTTGACCA ACAACCATCG GTCGGTCATT
    GCAGGATGTG GAGTCTCGCT TGTCAGCAAG AGCAAGTCCA ACCCCAACCT GCGGGATGGC
    GAGATGTGCA ATACAGATGA CGATTTGCAA CCCAGACCTC TGGTAGCCGA TAAAGACCCC
    ATCCGACCCA TGCAAAGGCG ACACACGTGG AGCAGGTTGT ACGTTGAAGG CCTAAAGCAG
    TCATCGACAC TTGCTGCTTC TGCAGCCTCC AAGAAGTCAA CTACCCTTTC AGCAAAAGAC
    ATCGACTCAA AGTCCAAAAG TCTGGGAGAT CTCACTTCAG ACGATATCAT CTGTAACTTC
    GAGAGCAAGT ATCGCAGCAT TAGCCGCAGT TTCATCGTGA GGCCAAGTCG CCAACATGGG
    AAAACTCTGA GGAAACCCCA AAATGATTTC ACTGAGCAGC TGAAGAGGCT CACTGACGTG
    GAACCCCTTA CCAGCAAGGA CTTTGCTCAG TGCCGGGCTG ACTCAGAAGC AGGACAGGAA
    GAAGAGGAGA TGCCTCCTTT ACGGAGGAGT TCATCACGGA GCCAGAGCCG CGTGAGATAC
    ATCGCTAACC GTGCCAGGCA GGCCCAGGAA AGGCAACGGC TACAAAGCAT AACCCGGATG
    TCTGGGAGCC CCATCGAAGA GCGTGGGAAC CCTGAAGGAG CTTGTAGTGT ACCCCATAGT
    CCTTGCACTT GCCTGGATCT TTTAAGCCAG CTTCCACCAG GACCACCCCA ACAAAGCCCA
    CTGAGCCCGG ACAATGAGGT CTTCTTTATG CTCAGGCTGT AA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.