capn15 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	capn15
Entrez Gene ID	100330198
Full Name	calpain 15
Synonyms	solh
General protein information	Preferred Names calpain 15 Names calpain-15 small optic lobes homolog zmp:0000001110
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002663847.6	XP_002663893.2	calpain-15 isoform X2
XM_005174197.3	XP_005174254.1	calpain-15 isoform X1
XM_002663847.5	XP_002663893.2	calpain-15 isoform X2
XM_005174197.4	XP_005174254.1	calpain-15 isoform X1
XM_021470406.1	XP_021326081.1	calpain-15 isoform X1

Pathways from BioSystems

Homologies

Rattus norvegicus (Norway rat)	Capn15	NP_001100460.1
Homo sapiens (human)	CAPN15	NP_005623.1
Bos taurus (cattle)	CAPN15	XP_005224641.1
Pan troglodytes (chimpanzee)	SOLH	XP_001172892.3
Mus musculus (house mouse)	Capn15	NP_056645.1
Gallus gallus (chicken)	SOLH	XP_004945483.1
Caenorhabditis elegans (roundworm)	W05G11.4	NP_497130.1
Canis lupus familiaris (dog)	CAPN15	XP_547218.3
Danio rerio (zebrafish)	solh	XP_005174254.1
Xenopus tropicalis (tropical clawed frog)	capn15	NP_001106511.1
	SOLH	XP_001085465.1
Caenorhabditis elegans (roundworm)	CELE_T11A5.6	NP_001256150.1

Gene Ontology

The following capn15 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the capn15 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa28887	XM_005174197.4 Latest version!	Danio rerio calpain 15 (capn15), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa28887	XM_021470406.1 Latest version!	Danio rerio calpain 15 (capn15), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa28888	XM_002663847.6 Latest version!	Danio rerio calpain 15 (capn15), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa28888	XM_002663847.5	Danio rerio calpain 15 (capn15), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa28887	XM_005174197.3	Danio rerio calpain 15 (capn15), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

capn15 ( XM_005174197.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005174254.1 Danio rerio calpain 15 (capn15), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa28887
Clone ID Related Accession (Same CDS sequence)	XM_005174197.3 , XM_005174197.4 , XM_021470406.1
Accession Version	XM_005174197.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calpain-15 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005174197.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGCT CTGCGTCGTC TTTGACCACG GATAGGGAAT CAGATCACGG CTTCGGAACG 
GCTCCGTTCC CCGCAGCTGC ACCGATGGGT TGGCTGAGGA CCAGTCACAG CAGTCCGGTG 
TGGGGAACCA CTTTCATCAC CCGCCAACAG CAGCAGCACC AGCAGCCTAT TCCACACAGA 
GAGCGCAGTC ATTCTCATTC ACCTGGTTCG ATGGAAGCAG TTGTGAGGAA TTCTGATTGG 
TCCTGCGGGC GCTGCACGTT CCTCAACTCC AGCGGCTCTA TGCGCTGCTC CATTTGTGAA 
GCTCCCCGAC AGAAGCCGGA TCTGAACCAC ATTCTGCGCC TCAGCAGTGC AGAGGAACCC 
CGCTGGTCCT GCCCACGCTG CACTCTCACC AACCACCACG GGTTAGGCTC CTGTTCTGTG 
TGTGGAGCTG CTCCGGTAAC ACCCAACGGA CCCCTCCCAC CCAAACAAAC CCCGCCCACT 
CCGATTGACC TCGCCTCTAG CCCTGAACCT AAAGGTCAGG TGGCGAATGG GGAATCCCAG 
TGCATAGAGT CCAATGGGGA GGTGCCTATG AGTGGGCAGG GCTCCTCAGA GTGGGCGTGT 
CCTCGTTGTA CTTTAGTGAA TACTCCAGTT GCGTTGTCGT GTTCAGCGTG TGGTGGTCCG 
AGGAAACTTT CCTTACCGAA AATCCCTCCG GAGGCTCTAG TGGTACCAGA AGTGCGCACA 
CCTGTGGCAG GGTTTCCTAC GCAAACGGCA GGAGCATCTG CACCTCTTCT TATCGATCTA 
ACCGAAGATT CTCCACCGCA GGCACCGTCT GAAACCCCAT CTCCACCCCC TGTCCCGTTT 
CTGCCCCCTT CGTTGTCCTC ACTTCAAAAT AATCCTGTGC CTCGCAGCCG AAGAGAAGTC 
CCGCCCCCTG GGCGGCCTCC AAACCCTGGC CCCTCACCAA CCTCACCTTC ATCCTGCCCA 
CCAACCAAGC CTAAACCCCT CCCACCATCA GGTAATTACC CAGTCAAGCG CCTCAGCGTG 
CTGGAGGAGG AGGAGCCTAA TAACCCCACC CCTCCAGTCA CATCTCCGCC TTCCTGGAAG 
TGCCCCGGAT GCTCCGCCCC AACAGCTCCT CCTTCATCGT CTGCCGGGCG CTGCGATGCA 
TGCAGAGGGT CGAGGCCGGG TTCGGGTGGT GATGTCATCG ATGTGCTCGG CGAATCAGTG 
CGTTTCACGC CAGCGTCGCC ATCCAGCCCA GACTTCAGCT CCTGGGCGTG CTCCAAATGT 
ACGCTGCGCA ACCCGACGGG CGCGGGTCAC TGTACGGCCT GCGGATCCTC CAAACTGCAT 
GGTTTCTCGG AGCCGTCCAG CTGCTCATCA TGCTCTCGGA AACAACCGCA CTCCCACCCG 
CGTGCCTTTA CCTCCACCTC CTCCTCGTCC TCCATTTCGT CCCCGTCGGC GCAGGACAAG 
AGTGTGTGCC AGTGGACGTG TCCTGCCTGC ACGCTGCTGA ACGAGGGAAG GATGAAGCAC 
TGCGCCGCAT GTCACACGCC GCAGCAGTAC CTGAATCTGA GGAAAACCGG GAAGCCGCTC 
AAGCGCAGGG AGAGCATGCA TGTGGAGGCC CGCAGGCGCA CGGATGAGGG AGAGGCCAAG 
GAATTGTGGG AAAATATCGT CAGCTTCTGT AGGGAGAACG CAGTGAATTT CGTGGATGAC 
AGTTTTCCTC CTGGTCCTCG ATCGGTCGGT TTCCCGGAAA ACGACAGCGT CCAGCAGCGG 
ATCAAGAAGT GGCTCCGTCC TCATGAGATC AACTGCAATA ACTTTAAGGA TCGCGGTGTG 
AAGTGGTCTG TGTTCAGGAC ACCCCGTCCC TCAGATATTC TGCAAGGCCT TCTGGGAAAC 
TGCTGGTTTC TGAGTGCGCT GGCGGTGTTG GCGGAGCGGC CGGAGTTGGT GGAGCGAGTG 
ATGATCACCA GAAGCATCTG TCAGGAAGGA GCCTATCAGG TCCGGCTCTG TAAAGACGGC 
ATGTGGACCA CCGTTTTAGT GGACGACATG CTGCCCTGTG ACGAATACGG CTTTCTGCTC 
TTCTCGCAGG CTCAGCGGAG GCAGCTGTGG GTTGCACTGA TAGAGAAGGC TCTGGCCAAG 
CTGCATGGCT CTTATTTTGC GCTTCAGGCC GGTCGAGCGA TCGAGGGTCT GGCAACGCTC 
ACCGGAGCCC CCTGTGAGAG TCTGATGCTG CAGGTCAGCT CAACAAACCC TCGAGAAGAG 
CCCATCGACA CTGACCTCAT CTGGGCCAAA ATGCTGAGCT CCAAAGAGGC CGGTTTCCTG 
ATGGGTGCGT CCTGTGGGGG CGGGAACATG AAGGTGGATG ATGCTGTGTA TGAATCTCTG 
GGGCTCCGCC CACGACATGC CTACTCCATA CTGGACGTCA GAGACGTGCA GGGCTACAGA 
CTGCTGCGTC TCAGGAACCC GTGGGGCCGG TTCTCCTGGA ACGGCTCCTG GTCGGACGAG 
TGGAGCGACT GGCCGCAGCA CCTCCGGCAC GAGCTCATGG CTCACGGCAG CAGTGAAGGC 
GTCTTCTGGA TGGAATACGG AGACTTCATT AAGTACTTTG ACTCGGTGGA CATCTGTAAG 
ATTCATCCAG ACTGGCAGGA GGTTCGTCTG CAGGGCTGTT TCCCCTGCCG GGCCAGTAAA 
CCGGTGACTG TAACGGCGCT TACGGTGCTG GAGAGAACGG CGCTGGAGTT CGCCCTCTTC 
CAGGAAGGCA GCAGGCGTTC AGACACGGCG GACAGTCACC TGCTGGACTT GTGCATCATG 
GTCTTCCGGG CCTCGTTCAG TAACGGGAAT AAGCTGGTTC TGGGTCGTCT GCTGGCACAC 
AGCAAGCGTG CGGTCAAGAA GTTTGTAGGC TGCGATGTGA TGCTGGAGCC GGGCGAGTAC 
GCCGTGGTTT GCTGTGCCTT CAACCACTGG CAGATGGACC TCAGCGGAGC CTCAACACCT 
GTGTCCAGCC CCACCAATGC TCGCAGACCC AGTCAGGATT TCCCAGGGTA CATTTTGGCC 
ATCTACAGCT CCAGACAGGT GATGGTGGAG CAGGTGGAGG CCACGTCCAC CACACTCGCA 
GACGCCATCA TCCTGCTGAC CGAGAACAAG GGGGAGAGAC ATGAGGGTCG GGAAGGAATG 
ACCTGTTATT ACCTGACCCA CGGGTGGGCG GGGCTTATCG TTGTGGTGGA GAATCGTCAC 
CCAAAGTACT ACCTCCATGT GTCCTGTGAC TGTACAGACA GTTTTAATGT GGTGTCGACA 
CGCGGCAGCC TGAAGACCAT CGACAGCGTT CCTCCTCTGC ACAGGCAGGT TCTGGTGGTT 
CTGTCTCAGC TGGAGGGAAA CGCCGGTTTC TCCATCACAC ACAGACTGGC TCACAGGAAA 
GCAGCACAGG CGTCTTTGGG CGACTGGACA TCCAGTAAAG CCACACACTC GCCCCAGCTC 
ACTCCAGACA CTGACGGACT GCACAGACCA CGGCCGCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005174254.1
CDS	1315..4776
Translation	MGSSASSLTTDRESDHGFGTAPFPAAAPMGWLRTSHSSPVWGTTFITRQQQQHQQPIPHRERSHSHSPGSMEAVVRNSDWSCGRCTFLNSSGSMRCSICEAPRQKPDLNHILRLSSAEEPRWSCPRCTLTNHHGLGSCSVCGAAPVTPNGPLPPKQTPPTPIDLASSPEPKGQVANGESQCIESNGEVPMSGQGSSEWACPRCTLVNTPVALSCSACGGPRKLSLPKIPPEALVVPEVRTPVAGFPTQTAGASAPLLIDLTEDSPPQAPSETPSPPPVPFLPPSLSSLQNNPVPRSRREVPPPGRPPNPGPSPTSPSSCPPTKPKPLPPSGNYPVKRLSVLEEEEPNNPTPPVTSPPSWKCPGCSAPTAPPSSSAGRCDACRGSRPGSGGDVIDVLGESVRFTPASPSSPDFSSWACSKCTLRNPTGAGHCTACGSSKLHGFSEPSSCSSCSRKQPHSHPRAFTSTSSSSSISSPSAQDKSVCQWTCPACTLLNEGRMKHCAACHTPQQYLNLRKTGKPLKRRESMHVEARRRTDEGEAKELWENIVSFCRENAVNFVDDSFPPGPRSVGFPENDSVQQRIKKWLRPHEINCNNFKDRGVKWSVFRTPRPSDILQGLLGNCWFLSALAVLAERPELVERVMITRSICQEGAYQVRLCKDGMWTTVLVDDMLPCDEYGFLLFSQAQRRQLWVALIEKALAKLHGSYFALQAGRAIEGLATLTGAPCESLMLQVSSTNPREEPIDTDLIWAKMLSSKEAGFLMGASCGGGNMKVDDAVYESLGLRPRHAYSILDVRDVQGYRLLRLRNPWGRFSWNGSWSDEWSDWPQHLRHELMAHGSSEGVFWMEYGDFIKYFDSVDICKIHPDWQEVRLQGCFPCRASKPVTVTALTVLERTALEFALFQEGSRRSDTADSHLLDLCIMVFRASFSNGNKLVLGRLLAHSKRAVKKFVGCDVMLEPGEYAVVCCAFNHWQMDLSGASTPVSSPTNARRPSQDFPGYILAIYSSRQVMVEQVEATSTTLADAIILLTENKGERHEGREGMTCYYLTHGWAGLIVVVENRHPKYYLHVSCDCTDSFNVVSTRGSLKTIDSVPPLHRQVLVVLSQLEGNAGFSITHRLAHRKAAQASLGDWTSSKATHSPQLTPDTDGLHRPRPL

Target ORF information:

RefSeq Version	XM_005174197.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calpain 15 (capn15), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005174197.3

ORF Insert Sequence:

ATGGGGAGCT CTGCGTCGTC TTTGACCACG GATAGGGAAT CAGATCACGG CTTCGGAACG 
GCTCCGTTCC CCGCAGCTGC ACCGATGGGT TGGCTGAGGA CCAGTCACAG CAGTCCGGTG 
TGGGGAACCA CTTTCATCAC CCGCCAACAG CAGCAGCACC AGCAGCCTAT TCCACACAGA 
GAGCGCAGTC ATTCTCATTC ACCTGGTTCG ATGGAAGCAG TTGTGAGGAA TTCTGATTGG 
TCCTGCGGGC GCTGCACGTT CCTCAACTCC AGCGGCTCTA TGCGCTGCTC CATTTGTGAA 
GCTCCCCGAC AGAAGCCGGA TCTGAACCAC ATTCTGCGCC TCAGCAGTGC AGAGGAACCC 
CGCTGGTCCT GCCCACGCTG CACTCTCACC AACCACCACG GGTTAGGCTC CTGTTCTGTG 
TGTGGAGCTG CTCCGGTAAC ACCCAACGGA CCCCTCCCAC CCAAACAAAC CCCGCCCACT 
CCGATTGACC TCGCCTCTAG CCCTGAACCT AAAGGTCAGG TGGCGAATGG GGAATCCCAG 
TGCATAGAGT CCAATGGGGA GGTGCCTATG AGTGGGCAGG GCTCCTCAGA GTGGGCGTGT 
CCTCGTTGTA CTTTAGTGAA TACTCCAGTT GCGTTGTCGT GTTCAGCGTG TGGTGGTCCG 
AGGAAACTTT CCTTACCGAA AATCCCTCCG GAGGCTCTAG TGGTACCAGA AGTGCGCACA 
CCTGTGGCAG GGTTTCCTAC GCAAACGGCA GGAGCATCTG CACCTCTTCT TATCGATCTA 
ACCGAAGATT CTCCACCGCA GGCACCGTCT GAAACCCCAT CTCCACCCCC TGTCCCGTTT 
CTGCCCCCTT CGTTGTCCTC ACTTCAAAAT AATCCTGTGC CTCGCAGCCG AAGAGAAGTC 
CCGCCCCCTG GGCGGCCTCC AAACCCTGGC CCCTCACCAA CCTCACCTTC ATCCTGCCCA 
CCAACCAAGC CTAAACCCCT CCCACCATCA GGTAATTACC CAGTCAAGCG CCTCAGCGTG 
CTGGAGGAGG AGGAGCCTAA TAACCCCACC CCTCCAGTCA CATCTCCGCC TTCCTGGAAG 
TGCCCCGGAT GCTCCGCCCC AACAGCTCCT CCTTCATCGT CTGCCGGGCG CTGCGATGCA 
TGCAGAGGGT CGAGGCCGGG TTCGGGTGGT GATGTCATCG ATGTGCTCGG CGAATCAGTG 
CGTTTCACGC CAGCGTCGCC ATCCAGCCCA GACTTCAGCT CCTGGGCGTG CTCCAAATGT 
ACGCTGCGCA ACCCGACGGG CGCGGGTCAC TGTACGGCCT GCGGATCCTC CAAACTGCAT 
GGTTTCTCGG AGCCGTCCAG CTGCTCATCA TGCTCTCGGA AACAACCGCA CTCCCACCCG 
CGTGCCTTTA CCTCCACCTC CTCCTCGTCC TCCATTTCGT CCCCGTCGGC GCAGGACAAG 
AGTGTGTGCC AGTGGACGTG TCCTGCCTGC ACGCTGCTGA ACGAGGGAAG GATGAAGCAC 
TGCGCCGCAT GTCACACGCC GCAGCAGTAC CTGAATCTGA GGAAAACCGG GAAGCCGCTC 
AAGCGCAGGG AGAGCATGCA TGTGGAGGCC CGCAGGCGCA CGGATGAGGG AGAGGCCAAG 
GAATTGTGGG AAAATATCGT CAGCTTCTGT AGGGAGAACG CAGTGAATTT CGTGGATGAC 
AGTTTTCCTC CTGGTCCTCG ATCGGTCGGT TTCCCGGAAA ACGACAGCGT CCAGCAGCGG 
ATCAAGAAGT GGCTCCGTCC TCATGAGATC AACTGCAATA ACTTTAAGGA TCGCGGTGTG 
AAGTGGTCTG TGTTCAGGAC ACCCCGTCCC TCAGATATTC TGCAAGGCCT TCTGGGAAAC 
TGCTGGTTTC TGAGTGCGCT GGCGGTGTTG GCGGAGCGGC CGGAGTTGGT GGAGCGAGTG 
ATGATCACCA GAAGCATCTG TCAGGAAGGA GCCTATCAGG TCCGGCTCTG TAAAGACGGC 
ATGTGGACCA CCGTTTTAGT GGACGACATG CTGCCCTGTG ACGAATACGG CTTTCTGCTC 
TTCTCGCAGG CTCAGCGGAG GCAGCTGTGG GTTGCACTGA TAGAGAAGGC TCTGGCCAAG 
CTGCATGGCT CTTATTTTGC GCTTCAGGCC GGTCGAGCGA TCGAGGGTCT GGCAACGCTC 
ACCGGAGCCC CCTGTGAGAG TCTGATGCTG CAGGTCAGCT CAACAAACCC TCGAGAAGAG 
CCCATCGACA CTGACCTCAT CTGGGCCAAA ATGCTGAGCT CCAAAGAGGC CGGTTTCCTG 
ATGGGTGCGT CCTGTGGGGG CGGGAACATG AAGGTGGATG ATGCTGTGTA TGAATCTCTG 
GGGCTCCGCC CACGACATGC CTACTCCATA CTGGACGTCA GAGACGTGCA GGGCTACAGA 
CTGCTGCGTC TCAGGAACCC GTGGGGCCGG TTCTCCTGGA ACGGCTCCTG GTCGGACGAG 
TGGAGCGACT GGCCGCAGCA CCTCCGGCAC GAGCTCATGG CTCACGGCAG CAGTGAAGGC 
GTCTTCTGGA TGGAATACGG AGACTTCATT AAGTACTTTG ACTCGGTGGA CATCTGTAAG 
ATTCATCCAG ACTGGCAGGA GGTTCGTCTG CAGGGCTGTT TCCCCTGCCG GGCCAGTAAA 
CCGGTGACTG TAACGGCGCT TACGGTGCTG GAGAGAACGG CGCTGGAGTT CGCCCTCTTC 
CAGGAAGGCA GCAGGCGTTC AGACACGGCG GACAGTCACC TGCTGGACTT GTGCATCATG 
GTCTTCCGGG CCTCGTTCAG TAACGGGAAT AAGCTGGTTC TGGGTCGTCT GCTGGCACAC 
AGCAAGCGTG CGGTCAAGAA GTTTGTAGGC TGCGATGTGA TGCTGGAGCC GGGCGAGTAC 
GCCGTGGTTT GCTGTGCCTT CAACCACTGG CAGATGGACC TCAGCGGAGC CTCAACACCT 
GTGTCCAGCC CCACCAATGC TCGCAGACCC AGTCAGGATT TCCCAGGGTA CATTTTGGCC 
ATCTACAGCT CCAGACAGGT GATGGTGGAG CAGGTGGAGG CCACGTCCAC CACACTCGCA 
GACGCCATCA TCCTGCTGAC CGAGAACAAG GGGGAGAGAC ATGAGGGTCG GGAAGGAATG 
ACCTGTTATT ACCTGACCCA CGGGTGGGCG GGGCTTATCG TTGTGGTGGA GAATCGTCAC 
CCAAAGTACT ACCTCCATGT GTCCTGTGAC TGTACAGACA GTTTTAATGT GGTGTCGACA 
CGCGGCAGCC TGAAGACCAT CGACAGCGTT CCTCCTCTGC ACAGGCAGGT TCTGGTGGTT 
CTGTCTCAGC TGGAGGGAAA CGCCGGTTTC TCCATCACAC ACAGACTGGC TCACAGGAAA 
GCAGCACAGG CGTCTTTGGG CGACTGGACA TCCAGTAAAG CCACACACTC GCCCCAGCTC 
ACTCCAGACA CTGACGGACT GCACAGACCA CGGCCGCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

capn15 ( XM_005174197.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005174254.1 Danio rerio calpain 15 (capn15), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa28887
Clone ID Related Accession (Same CDS sequence)	XM_005174197.3 , XM_005174197.4 , XM_021470406.1
Accession Version	XM_005174197.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpain-15 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005174197.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGCT CTGCGTCGTC TTTGACCACG GATAGGGAAT CAGATCACGG CTTCGGAACG 
GCTCCGTTCC CCGCAGCTGC ACCGATGGGT TGGCTGAGGA CCAGTCACAG CAGTCCGGTG 
TGGGGAACCA CTTTCATCAC CCGCCAACAG CAGCAGCACC AGCAGCCTAT TCCACACAGA 
GAGCGCAGTC ATTCTCATTC ACCTGGTTCG ATGGAAGCAG TTGTGAGGAA TTCTGATTGG 
TCCTGCGGGC GCTGCACGTT CCTCAACTCC AGCGGCTCTA TGCGCTGCTC CATTTGTGAA 
GCTCCCCGAC AGAAGCCGGA TCTGAACCAC ATTCTGCGCC TCAGCAGTGC AGAGGAACCC 
CGCTGGTCCT GCCCACGCTG CACTCTCACC AACCACCACG GGTTAGGCTC CTGTTCTGTG 
TGTGGAGCTG CTCCGGTAAC ACCCAACGGA CCCCTCCCAC CCAAACAAAC CCCGCCCACT 
CCGATTGACC TCGCCTCTAG CCCTGAACCT AAAGGTCAGG TGGCGAATGG GGAATCCCAG 
TGCATAGAGT CCAATGGGGA GGTGCCTATG AGTGGGCAGG GCTCCTCAGA GTGGGCGTGT 
CCTCGTTGTA CTTTAGTGAA TACTCCAGTT GCGTTGTCGT GTTCAGCGTG TGGTGGTCCG 
AGGAAACTTT CCTTACCGAA AATCCCTCCG GAGGCTCTAG TGGTACCAGA AGTGCGCACA 
CCTGTGGCAG GGTTTCCTAC GCAAACGGCA GGAGCATCTG CACCTCTTCT TATCGATCTA 
ACCGAAGATT CTCCACCGCA GGCACCGTCT GAAACCCCAT CTCCACCCCC TGTCCCGTTT 
CTGCCCCCTT CGTTGTCCTC ACTTCAAAAT AATCCTGTGC CTCGCAGCCG AAGAGAAGTC 
CCGCCCCCTG GGCGGCCTCC AAACCCTGGC CCCTCACCAA CCTCACCTTC ATCCTGCCCA 
CCAACCAAGC CTAAACCCCT CCCACCATCA GGTAATTACC CAGTCAAGCG CCTCAGCGTG 
CTGGAGGAGG AGGAGCCTAA TAACCCCACC CCTCCAGTCA CATCTCCGCC TTCCTGGAAG 
TGCCCCGGAT GCTCCGCCCC AACAGCTCCT CCTTCATCGT CTGCCGGGCG CTGCGATGCA 
TGCAGAGGGT CGAGGCCGGG TTCGGGTGGT GATGTCATCG ATGTGCTCGG CGAATCAGTG 
CGTTTCACGC CAGCGTCGCC ATCCAGCCCA GACTTCAGCT CCTGGGCGTG CTCCAAATGT 
ACGCTGCGCA ACCCGACGGG CGCGGGTCAC TGTACGGCCT GCGGATCCTC CAAACTGCAT 
GGTTTCTCGG AGCCGTCCAG CTGCTCATCA TGCTCTCGGA AACAACCGCA CTCCCACCCG 
CGTGCCTTTA CCTCCACCTC CTCCTCGTCC TCCATTTCGT CCCCGTCGGC GCAGGACAAG 
AGTGTGTGCC AGTGGACGTG TCCTGCCTGC ACGCTGCTGA ACGAGGGAAG GATGAAGCAC 
TGCGCCGCAT GTCACACGCC GCAGCAGTAC CTGAATCTGA GGAAAACCGG GAAGCCGCTC 
AAGCGCAGGG AGAGCATGCA TGTGGAGGCC CGCAGGCGCA CGGATGAGGG AGAGGCCAAG 
GAATTGTGGG AAAATATCGT CAGCTTCTGT AGGGAGAACG CAGTGAATTT CGTGGATGAC 
AGTTTTCCTC CTGGTCCTCG ATCGGTCGGT TTCCCGGAAA ACGACAGCGT CCAGCAGCGG 
ATCAAGAAGT GGCTCCGTCC TCATGAGATC AACTGCAATA ACTTTAAGGA TCGCGGTGTG 
AAGTGGTCTG TGTTCAGGAC ACCCCGTCCC TCAGATATTC TGCAAGGCCT TCTGGGAAAC 
TGCTGGTTTC TGAGTGCGCT GGCGGTGTTG GCGGAGCGGC CGGAGTTGGT GGAGCGAGTG 
ATGATCACCA GAAGCATCTG TCAGGAAGGA GCCTATCAGG TCCGGCTCTG TAAAGACGGC 
ATGTGGACCA CCGTTTTAGT GGACGACATG CTGCCCTGTG ACGAATACGG CTTTCTGCTC 
TTCTCGCAGG CTCAGCGGAG GCAGCTGTGG GTTGCACTGA TAGAGAAGGC TCTGGCCAAG 
CTGCATGGCT CTTATTTTGC GCTTCAGGCC GGTCGAGCGA TCGAGGGTCT GGCAACGCTC 
ACCGGAGCCC CCTGTGAGAG TCTGATGCTG CAGGTCAGCT CAACAAACCC TCGAGAAGAG 
CCCATCGACA CTGACCTCAT CTGGGCCAAA ATGCTGAGCT CCAAAGAGGC CGGTTTCCTG 
ATGGGTGCGT CCTGTGGGGG CGGGAACATG AAGGTGGATG ATGCTGTGTA TGAATCTCTG 
GGGCTCCGCC CACGACATGC CTACTCCATA CTGGACGTCA GAGACGTGCA GGGCTACAGA 
CTGCTGCGTC TCAGGAACCC GTGGGGCCGG TTCTCCTGGA ACGGCTCCTG GTCGGACGAG 
TGGAGCGACT GGCCGCAGCA CCTCCGGCAC GAGCTCATGG CTCACGGCAG CAGTGAAGGC 
GTCTTCTGGA TGGAATACGG AGACTTCATT AAGTACTTTG ACTCGGTGGA CATCTGTAAG 
ATTCATCCAG ACTGGCAGGA GGTTCGTCTG CAGGGCTGTT TCCCCTGCCG GGCCAGTAAA 
CCGGTGACTG TAACGGCGCT TACGGTGCTG GAGAGAACGG CGCTGGAGTT CGCCCTCTTC 
CAGGAAGGCA GCAGGCGTTC AGACACGGCG GACAGTCACC TGCTGGACTT GTGCATCATG 
GTCTTCCGGG CCTCGTTCAG TAACGGGAAT AAGCTGGTTC TGGGTCGTCT GCTGGCACAC 
AGCAAGCGTG CGGTCAAGAA GTTTGTAGGC TGCGATGTGA TGCTGGAGCC GGGCGAGTAC 
GCCGTGGTTT GCTGTGCCTT CAACCACTGG CAGATGGACC TCAGCGGAGC CTCAACACCT 
GTGTCCAGCC CCACCAATGC TCGCAGACCC AGTCAGGATT TCCCAGGGTA CATTTTGGCC 
ATCTACAGCT CCAGACAGGT GATGGTGGAG CAGGTGGAGG CCACGTCCAC CACACTCGCA 
GACGCCATCA TCCTGCTGAC CGAGAACAAG GGGGAGAGAC ATGAGGGTCG GGAAGGAATG 
ACCTGTTATT ACCTGACCCA CGGGTGGGCG GGGCTTATCG TTGTGGTGGA GAATCGTCAC 
CCAAAGTACT ACCTCCATGT GTCCTGTGAC TGTACAGACA GTTTTAATGT GGTGTCGACA 
CGCGGCAGCC TGAAGACCAT CGACAGCGTT CCTCCTCTGC ACAGGCAGGT TCTGGTGGTT 
CTGTCTCAGC TGGAGGGAAA CGCCGGTTTC TCCATCACAC ACAGACTGGC TCACAGGAAA 
GCAGCACAGG CGTCTTTGGG CGACTGGACA TCCAGTAAAG CCACACACTC GCCCCAGCTC 
ACTCCAGACA CTGACGGACT GCACAGACCA CGGCCGCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005174254.1
CDS	1324..4785
Translation	MGSSASSLTTDRESDHGFGTAPFPAAAPMGWLRTSHSSPVWGTTFITRQQQQHQQPIPHRERSHSHSPGSMEAVVRNSDWSCGRCTFLNSSGSMRCSICEAPRQKPDLNHILRLSSAEEPRWSCPRCTLTNHHGLGSCSVCGAAPVTPNGPLPPKQTPPTPIDLASSPEPKGQVANGESQCIESNGEVPMSGQGSSEWACPRCTLVNTPVALSCSACGGPRKLSLPKIPPEALVVPEVRTPVAGFPTQTAGASAPLLIDLTEDSPPQAPSETPSPPPVPFLPPSLSSLQNNPVPRSRREVPPPGRPPNPGPSPTSPSSCPPTKPKPLPPSGNYPVKRLSVLEEEEPNNPTPPVTSPPSWKCPGCSAPTAPPSSSAGRCDACRGSRPGSGGDVIDVLGESVRFTPASPSSPDFSSWACSKCTLRNPTGAGHCTACGSSKLHGFSEPSSCSSCSRKQPHSHPRAFTSTSSSSSISSPSAQDKSVCQWTCPACTLLNEGRMKHCAACHTPQQYLNLRKTGKPLKRRESMHVEARRRTDEGEAKELWENIVSFCRENAVNFVDDSFPPGPRSVGFPENDSVQQRIKKWLRPHEINCNNFKDRGVKWSVFRTPRPSDILQGLLGNCWFLSALAVLAERPELVERVMITRSICQEGAYQVRLCKDGMWTTVLVDDMLPCDEYGFLLFSQAQRRQLWVALIEKALAKLHGSYFALQAGRAIEGLATLTGAPCESLMLQVSSTNPREEPIDTDLIWAKMLSSKEAGFLMGASCGGGNMKVDDAVYESLGLRPRHAYSILDVRDVQGYRLLRLRNPWGRFSWNGSWSDEWSDWPQHLRHELMAHGSSEGVFWMEYGDFIKYFDSVDICKIHPDWQEVRLQGCFPCRASKPVTVTALTVLERTALEFALFQEGSRRSDTADSHLLDLCIMVFRASFSNGNKLVLGRLLAHSKRAVKKFVGCDVMLEPGEYAVVCCAFNHWQMDLSGASTPVSSPTNARRPSQDFPGYILAIYSSRQVMVEQVEATSTTLADAIILLTENKGERHEGREGMTCYYLTHGWAGLIVVVENRHPKYYLHVSCDCTDSFNVVSTRGSLKTIDSVPPLHRQVLVVLSQLEGNAGFSITHRLAHRKAAQASLGDWTSSKATHSPQLTPDTDGLHRPRPL

Target ORF information:

RefSeq Version	XM_005174197.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpain 15 (capn15), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005174197.4

ORF Insert Sequence:

ATGGGGAGCT CTGCGTCGTC TTTGACCACG GATAGGGAAT CAGATCACGG CTTCGGAACG 
GCTCCGTTCC CCGCAGCTGC ACCGATGGGT TGGCTGAGGA CCAGTCACAG CAGTCCGGTG 
TGGGGAACCA CTTTCATCAC CCGCCAACAG CAGCAGCACC AGCAGCCTAT TCCACACAGA 
GAGCGCAGTC ATTCTCATTC ACCTGGTTCG ATGGAAGCAG TTGTGAGGAA TTCTGATTGG 
TCCTGCGGGC GCTGCACGTT CCTCAACTCC AGCGGCTCTA TGCGCTGCTC CATTTGTGAA 
GCTCCCCGAC AGAAGCCGGA TCTGAACCAC ATTCTGCGCC TCAGCAGTGC AGAGGAACCC 
CGCTGGTCCT GCCCACGCTG CACTCTCACC AACCACCACG GGTTAGGCTC CTGTTCTGTG 
TGTGGAGCTG CTCCGGTAAC ACCCAACGGA CCCCTCCCAC CCAAACAAAC CCCGCCCACT 
CCGATTGACC TCGCCTCTAG CCCTGAACCT AAAGGTCAGG TGGCGAATGG GGAATCCCAG 
TGCATAGAGT CCAATGGGGA GGTGCCTATG AGTGGGCAGG GCTCCTCAGA GTGGGCGTGT 
CCTCGTTGTA CTTTAGTGAA TACTCCAGTT GCGTTGTCGT GTTCAGCGTG TGGTGGTCCG 
AGGAAACTTT CCTTACCGAA AATCCCTCCG GAGGCTCTAG TGGTACCAGA AGTGCGCACA 
CCTGTGGCAG GGTTTCCTAC GCAAACGGCA GGAGCATCTG CACCTCTTCT TATCGATCTA 
ACCGAAGATT CTCCACCGCA GGCACCGTCT GAAACCCCAT CTCCACCCCC TGTCCCGTTT 
CTGCCCCCTT CGTTGTCCTC ACTTCAAAAT AATCCTGTGC CTCGCAGCCG AAGAGAAGTC 
CCGCCCCCTG GGCGGCCTCC AAACCCTGGC CCCTCACCAA CCTCACCTTC ATCCTGCCCA 
CCAACCAAGC CTAAACCCCT CCCACCATCA GGTAATTACC CAGTCAAGCG CCTCAGCGTG 
CTGGAGGAGG AGGAGCCTAA TAACCCCACC CCTCCAGTCA CATCTCCGCC TTCCTGGAAG 
TGCCCCGGAT GCTCCGCCCC AACAGCTCCT CCTTCATCGT CTGCCGGGCG CTGCGATGCA 
TGCAGAGGGT CGAGGCCGGG TTCGGGTGGT GATGTCATCG ATGTGCTCGG CGAATCAGTG 
CGTTTCACGC CAGCGTCGCC ATCCAGCCCA GACTTCAGCT CCTGGGCGTG CTCCAAATGT 
ACGCTGCGCA ACCCGACGGG CGCGGGTCAC TGTACGGCCT GCGGATCCTC CAAACTGCAT 
GGTTTCTCGG AGCCGTCCAG CTGCTCATCA TGCTCTCGGA AACAACCGCA CTCCCACCCG 
CGTGCCTTTA CCTCCACCTC CTCCTCGTCC TCCATTTCGT CCCCGTCGGC GCAGGACAAG 
AGTGTGTGCC AGTGGACGTG TCCTGCCTGC ACGCTGCTGA ACGAGGGAAG GATGAAGCAC 
TGCGCCGCAT GTCACACGCC GCAGCAGTAC CTGAATCTGA GGAAAACCGG GAAGCCGCTC 
AAGCGCAGGG AGAGCATGCA TGTGGAGGCC CGCAGGCGCA CGGATGAGGG AGAGGCCAAG 
GAATTGTGGG AAAATATCGT CAGCTTCTGT AGGGAGAACG CAGTGAATTT CGTGGATGAC 
AGTTTTCCTC CTGGTCCTCG ATCGGTCGGT TTCCCGGAAA ACGACAGCGT CCAGCAGCGG 
ATCAAGAAGT GGCTCCGTCC TCATGAGATC AACTGCAATA ACTTTAAGGA TCGCGGTGTG 
AAGTGGTCTG TGTTCAGGAC ACCCCGTCCC TCAGATATTC TGCAAGGCCT TCTGGGAAAC 
TGCTGGTTTC TGAGTGCGCT GGCGGTGTTG GCGGAGCGGC CGGAGTTGGT GGAGCGAGTG 
ATGATCACCA GAAGCATCTG TCAGGAAGGA GCCTATCAGG TCCGGCTCTG TAAAGACGGC 
ATGTGGACCA CCGTTTTAGT GGACGACATG CTGCCCTGTG ACGAATACGG CTTTCTGCTC 
TTCTCGCAGG CTCAGCGGAG GCAGCTGTGG GTTGCACTGA TAGAGAAGGC TCTGGCCAAG 
CTGCATGGCT CTTATTTTGC GCTTCAGGCC GGTCGAGCGA TCGAGGGTCT GGCAACGCTC 
ACCGGAGCCC CCTGTGAGAG TCTGATGCTG CAGGTCAGCT CAACAAACCC TCGAGAAGAG 
CCCATCGACA CTGACCTCAT CTGGGCCAAA ATGCTGAGCT CCAAAGAGGC CGGTTTCCTG 
ATGGGTGCGT CCTGTGGGGG CGGGAACATG AAGGTGGATG ATGCTGTGTA TGAATCTCTG 
GGGCTCCGCC CACGACATGC CTACTCCATA CTGGACGTCA GAGACGTGCA GGGCTACAGA 
CTGCTGCGTC TCAGGAACCC GTGGGGCCGG TTCTCCTGGA ACGGCTCCTG GTCGGACGAG 
TGGAGCGACT GGCCGCAGCA CCTCCGGCAC GAGCTCATGG CTCACGGCAG CAGTGAAGGC 
GTCTTCTGGA TGGAATACGG AGACTTCATT AAGTACTTTG ACTCGGTGGA CATCTGTAAG 
ATTCATCCAG ACTGGCAGGA GGTTCGTCTG CAGGGCTGTT TCCCCTGCCG GGCCAGTAAA 
CCGGTGACTG TAACGGCGCT TACGGTGCTG GAGAGAACGG CGCTGGAGTT CGCCCTCTTC 
CAGGAAGGCA GCAGGCGTTC AGACACGGCG GACAGTCACC TGCTGGACTT GTGCATCATG 
GTCTTCCGGG CCTCGTTCAG TAACGGGAAT AAGCTGGTTC TGGGTCGTCT GCTGGCACAC 
AGCAAGCGTG CGGTCAAGAA GTTTGTAGGC TGCGATGTGA TGCTGGAGCC GGGCGAGTAC 
GCCGTGGTTT GCTGTGCCTT CAACCACTGG CAGATGGACC TCAGCGGAGC CTCAACACCT 
GTGTCCAGCC CCACCAATGC TCGCAGACCC AGTCAGGATT TCCCAGGGTA CATTTTGGCC 
ATCTACAGCT CCAGACAGGT GATGGTGGAG CAGGTGGAGG CCACGTCCAC CACACTCGCA 
GACGCCATCA TCCTGCTGAC CGAGAACAAG GGGGAGAGAC ATGAGGGTCG GGAAGGAATG 
ACCTGTTATT ACCTGACCCA CGGGTGGGCG GGGCTTATCG TTGTGGTGGA GAATCGTCAC 
CCAAAGTACT ACCTCCATGT GTCCTGTGAC TGTACAGACA GTTTTAATGT GGTGTCGACA 
CGCGGCAGCC TGAAGACCAT CGACAGCGTT CCTCCTCTGC ACAGGCAGGT TCTGGTGGTT 
CTGTCTCAGC TGGAGGGAAA CGCCGGTTTC TCCATCACAC ACAGACTGGC TCACAGGAAA 
GCAGCACAGG CGTCTTTGGG CGACTGGACA TCCAGTAAAG CCACACACTC GCCCCAGCTC 
ACTCCAGACA CTGACGGACT GCACAGACCA CGGCCGCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

capn15 ( XM_021470406.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326081.1 Danio rerio calpain 15 (capn15), transcript variant X2, mRNA.

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CloneID	ODa28887
Clone ID Related Accession (Same CDS sequence)	XM_005174197.3 , XM_005174197.4 , XM_021470406.1
Accession Version	XM_021470406.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3462bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpain-15 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGGGAGCT CTGCGTCGTC TTTGACCACG GATAGGGAAT CAGATCACGG CTTCGGAACG 
GCTCCGTTCC CCGCAGCTGC ACCGATGGGT TGGCTGAGGA CCAGTCACAG CAGTCCGGTG 
TGGGGAACCA CTTTCATCAC CCGCCAACAG CAGCAGCACC AGCAGCCTAT TCCACACAGA 
GAGCGCAGTC ATTCTCATTC ACCTGGTTCG ATGGAAGCAG TTGTGAGGAA TTCTGATTGG 
TCCTGCGGGC GCTGCACGTT CCTCAACTCC AGCGGCTCTA TGCGCTGCTC CATTTGTGAA 
GCTCCCCGAC AGAAGCCGGA TCTGAACCAC ATTCTGCGCC TCAGCAGTGC AGAGGAACCC 
CGCTGGTCCT GCCCACGCTG CACTCTCACC AACCACCACG GGTTAGGCTC CTGTTCTGTG 
TGTGGAGCTG CTCCGGTAAC ACCCAACGGA CCCCTCCCAC CCAAACAAAC CCCGCCCACT 
CCGATTGACC TCGCCTCTAG CCCTGAACCT AAAGGTCAGG TGGCGAATGG GGAATCCCAG 
TGCATAGAGT CCAATGGGGA GGTGCCTATG AGTGGGCAGG GCTCCTCAGA GTGGGCGTGT 
CCTCGTTGTA CTTTAGTGAA TACTCCAGTT GCGTTGTCGT GTTCAGCGTG TGGTGGTCCG 
AGGAAACTTT CCTTACCGAA AATCCCTCCG GAGGCTCTAG TGGTACCAGA AGTGCGCACA 
CCTGTGGCAG GGTTTCCTAC GCAAACGGCA GGAGCATCTG CACCTCTTCT TATCGATCTA 
ACCGAAGATT CTCCACCGCA GGCACCGTCT GAAACCCCAT CTCCACCCCC TGTCCCGTTT 
CTGCCCCCTT CGTTGTCCTC ACTTCAAAAT AATCCTGTGC CTCGCAGCCG AAGAGAAGTC 
CCGCCCCCTG GGCGGCCTCC AAACCCTGGC CCCTCACCAA CCTCACCTTC ATCCTGCCCA 
CCAACCAAGC CTAAACCCCT CCCACCATCA GGTAATTACC CAGTCAAGCG CCTCAGCGTG 
CTGGAGGAGG AGGAGCCTAA TAACCCCACC CCTCCAGTCA CATCTCCGCC TTCCTGGAAG 
TGCCCCGGAT GCTCCGCCCC AACAGCTCCT CCTTCATCGT CTGCCGGGCG CTGCGATGCA 
TGCAGAGGGT CGAGGCCGGG TTCGGGTGGT GATGTCATCG ATGTGCTCGG CGAATCAGTG 
CGTTTCACGC CAGCGTCGCC ATCCAGCCCA GACTTCAGCT CCTGGGCGTG CTCCAAATGT 
ACGCTGCGCA ACCCGACGGG CGCGGGTCAC TGTACGGCCT GCGGATCCTC CAAACTGCAT 
GGTTTCTCGG AGCCGTCCAG CTGCTCATCA TGCTCTCGGA AACAACCGCA CTCCCACCCG 
CGTGCCTTTA CCTCCACCTC CTCCTCGTCC TCCATTTCGT CCCCGTCGGC GCAGGACAAG 
AGTGTGTGCC AGTGGACGTG TCCTGCCTGC ACGCTGCTGA ACGAGGGAAG GATGAAGCAC 
TGCGCCGCAT GTCACACGCC GCAGCAGTAC CTGAATCTGA GGAAAACCGG GAAGCCGCTC 
AAGCGCAGGG AGAGCATGCA TGTGGAGGCC CGCAGGCGCA CGGATGAGGG AGAGGCCAAG 
GAATTGTGGG AAAATATCGT CAGCTTCTGT AGGGAGAACG CAGTGAATTT CGTGGATGAC 
AGTTTTCCTC CTGGTCCTCG ATCGGTCGGT TTCCCGGAAA ACGACAGCGT CCAGCAGCGG 
ATCAAGAAGT GGCTCCGTCC TCATGAGATC AACTGCAATA ACTTTAAGGA TCGCGGTGTG 
AAGTGGTCTG TGTTCAGGAC ACCCCGTCCC TCAGATATTC TGCAAGGCCT TCTGGGAAAC 
TGCTGGTTTC TGAGTGCGCT GGCGGTGTTG GCGGAGCGGC CGGAGTTGGT GGAGCGAGTG 
ATGATCACCA GAAGCATCTG TCAGGAAGGA GCCTATCAGG TCCGGCTCTG TAAAGACGGC 
ATGTGGACCA CCGTTTTAGT GGACGACATG CTGCCCTGTG ACGAATACGG CTTTCTGCTC 
TTCTCGCAGG CTCAGCGGAG GCAGCTGTGG GTTGCACTGA TAGAGAAGGC TCTGGCCAAG 
CTGCATGGCT CTTATTTTGC GCTTCAGGCC GGTCGAGCGA TCGAGGGTCT GGCAACGCTC 
ACCGGAGCCC CCTGTGAGAG TCTGATGCTG CAGGTCAGCT CAACAAACCC TCGAGAAGAG 
CCCATCGACA CTGACCTCAT CTGGGCCAAA ATGCTGAGCT CCAAAGAGGC CGGTTTCCTG 
ATGGGTGCGT CCTGTGGGGG CGGGAACATG AAGGTGGATG ATGCTGTGTA TGAATCTCTG 
GGGCTCCGCC CACGACATGC CTACTCCATA CTGGACGTCA GAGACGTGCA GGGCTACAGA 
CTGCTGCGTC TCAGGAACCC GTGGGGCCGG TTCTCCTGGA ACGGCTCCTG GTCGGACGAG 
TGGAGCGACT GGCCGCAGCA CCTCCGGCAC GAGCTCATGG CTCACGGCAG CAGTGAAGGC 
GTCTTCTGGA TGGAATACGG AGACTTCATT AAGTACTTTG ACTCGGTGGA CATCTGTAAG 
ATTCATCCAG ACTGGCAGGA GGTTCGTCTG CAGGGCTGTT TCCCCTGCCG GGCCAGTAAA 
CCGGTGACTG TAACGGCGCT TACGGTGCTG GAGAGAACGG CGCTGGAGTT CGCCCTCTTC 
CAGGAAGGCA GCAGGCGTTC AGACACGGCG GACAGTCACC TGCTGGACTT GTGCATCATG 
GTCTTCCGGG CCTCGTTCAG TAACGGGAAT AAGCTGGTTC TGGGTCGTCT GCTGGCACAC 
AGCAAGCGTG CGGTCAAGAA GTTTGTAGGC TGCGATGTGA TGCTGGAGCC GGGCGAGTAC 
GCCGTGGTTT GCTGTGCCTT CAACCACTGG CAGATGGACC TCAGCGGAGC CTCAACACCT 
GTGTCCAGCC CCACCAATGC TCGCAGACCC AGTCAGGATT TCCCAGGGTA CATTTTGGCC 
ATCTACAGCT CCAGACAGGT GATGGTGGAG CAGGTGGAGG CCACGTCCAC CACACTCGCA 
GACGCCATCA TCCTGCTGAC CGAGAACAAG GGGGAGAGAC ATGAGGGTCG GGAAGGAATG 
ACCTGTTATT ACCTGACCCA CGGGTGGGCG GGGCTTATCG TTGTGGTGGA GAATCGTCAC 
CCAAAGTACT ACCTCCATGT GTCCTGTGAC TGTACAGACA GTTTTAATGT GGTGTCGACA 
CGCGGCAGCC TGAAGACCAT CGACAGCGTT CCTCCTCTGC ACAGGCAGGT TCTGGTGGTT 
CTGTCTCAGC TGGAGGGAAA CGCCGGTTTC TCCATCACAC ACAGACTGGC TCACAGGAAA 
GCAGCACAGG CGTCTTTGGG CGACTGGACA TCCAGTAAAG CCACACACTC GCCCCAGCTC 
ACTCCAGACA CTGACGGACT GCACAGACCA CGGCCGCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326081.1
CDS	1942..5403
Translation	MGSSASSLTTDRESDHGFGTAPFPAAAPMGWLRTSHSSPVWGTTFITRQQQQHQQPIPHRERSHSHSPGSMEAVVRNSDWSCGRCTFLNSSGSMRCSICEAPRQKPDLNHILRLSSAEEPRWSCPRCTLTNHHGLGSCSVCGAAPVTPNGPLPPKQTPPTPIDLASSPEPKGQVANGESQCIESNGEVPMSGQGSSEWACPRCTLVNTPVALSCSACGGPRKLSLPKIPPEALVVPEVRTPVAGFPTQTAGASAPLLIDLTEDSPPQAPSETPSPPPVPFLPPSLSSLQNNPVPRSRREVPPPGRPPNPGPSPTSPSSCPPTKPKPLPPSGNYPVKRLSVLEEEEPNNPTPPVTSPPSWKCPGCSAPTAPPSSSAGRCDACRGSRPGSGGDVIDVLGESVRFTPASPSSPDFSSWACSKCTLRNPTGAGHCTACGSSKLHGFSEPSSCSSCSRKQPHSHPRAFTSTSSSSSISSPSAQDKSVCQWTCPACTLLNEGRMKHCAACHTPQQYLNLRKTGKPLKRRESMHVEARRRTDEGEAKELWENIVSFCRENAVNFVDDSFPPGPRSVGFPENDSVQQRIKKWLRPHEINCNNFKDRGVKWSVFRTPRPSDILQGLLGNCWFLSALAVLAERPELVERVMITRSICQEGAYQVRLCKDGMWTTVLVDDMLPCDEYGFLLFSQAQRRQLWVALIEKALAKLHGSYFALQAGRAIEGLATLTGAPCESLMLQVSSTNPREEPIDTDLIWAKMLSSKEAGFLMGASCGGGNMKVDDAVYESLGLRPRHAYSILDVRDVQGYRLLRLRNPWGRFSWNGSWSDEWSDWPQHLRHELMAHGSSEGVFWMEYGDFIKYFDSVDICKIHPDWQEVRLQGCFPCRASKPVTVTALTVLERTALEFALFQEGSRRSDTADSHLLDLCIMVFRASFSNGNKLVLGRLLAHSKRAVKKFVGCDVMLEPGEYAVVCCAFNHWQMDLSGASTPVSSPTNARRPSQDFPGYILAIYSSRQVMVEQVEATSTTLADAIILLTENKGERHEGREGMTCYYLTHGWAGLIVVVENRHPKYYLHVSCDCTDSFNVVSTRGSLKTIDSVPPLHRQVLVVLSQLEGNAGFSITHRLAHRKAAQASLGDWTSSKATHSPQLTPDTDGLHRPRPL

Target ORF information:

RefSeq Version	XM_021470406.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpain 15 (capn15), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470406.1

ORF Insert Sequence:

ATGGGGAGCT CTGCGTCGTC TTTGACCACG GATAGGGAAT CAGATCACGG CTTCGGAACG 
GCTCCGTTCC CCGCAGCTGC ACCGATGGGT TGGCTGAGGA CCAGTCACAG CAGTCCGGTG 
TGGGGAACCA CTTTCATCAC CCGCCAACAG CAGCAGCACC AGCAGCCTAT TCCACACAGA 
GAGCGCAGTC ATTCTCATTC ACCTGGTTCG ATGGAAGCAG TTGTGAGGAA TTCTGATTGG 
TCCTGCGGGC GCTGCACGTT CCTCAACTCC AGCGGCTCTA TGCGCTGCTC CATTTGTGAA 
GCTCCCCGAC AGAAGCCGGA TCTGAACCAC ATTCTGCGCC TCAGCAGTGC AGAGGAACCC 
CGCTGGTCCT GCCCACGCTG CACTCTCACC AACCACCACG GGTTAGGCTC CTGTTCTGTG 
TGTGGAGCTG CTCCGGTAAC ACCCAACGGA CCCCTCCCAC CCAAACAAAC CCCGCCCACT 
CCGATTGACC TCGCCTCTAG CCCTGAACCT AAAGGTCAGG TGGCGAATGG GGAATCCCAG 
TGCATAGAGT CCAATGGGGA GGTGCCTATG AGTGGGCAGG GCTCCTCAGA GTGGGCGTGT 
CCTCGTTGTA CTTTAGTGAA TACTCCAGTT GCGTTGTCGT GTTCAGCGTG TGGTGGTCCG 
AGGAAACTTT CCTTACCGAA AATCCCTCCG GAGGCTCTAG TGGTACCAGA AGTGCGCACA 
CCTGTGGCAG GGTTTCCTAC GCAAACGGCA GGAGCATCTG CACCTCTTCT TATCGATCTA 
ACCGAAGATT CTCCACCGCA GGCACCGTCT GAAACCCCAT CTCCACCCCC TGTCCCGTTT 
CTGCCCCCTT CGTTGTCCTC ACTTCAAAAT AATCCTGTGC CTCGCAGCCG AAGAGAAGTC 
CCGCCCCCTG GGCGGCCTCC AAACCCTGGC CCCTCACCAA CCTCACCTTC ATCCTGCCCA 
CCAACCAAGC CTAAACCCCT CCCACCATCA GGTAATTACC CAGTCAAGCG CCTCAGCGTG 
CTGGAGGAGG AGGAGCCTAA TAACCCCACC CCTCCAGTCA CATCTCCGCC TTCCTGGAAG 
TGCCCCGGAT GCTCCGCCCC AACAGCTCCT CCTTCATCGT CTGCCGGGCG CTGCGATGCA 
TGCAGAGGGT CGAGGCCGGG TTCGGGTGGT GATGTCATCG ATGTGCTCGG CGAATCAGTG 
CGTTTCACGC CAGCGTCGCC ATCCAGCCCA GACTTCAGCT CCTGGGCGTG CTCCAAATGT 
ACGCTGCGCA ACCCGACGGG CGCGGGTCAC TGTACGGCCT GCGGATCCTC CAAACTGCAT 
GGTTTCTCGG AGCCGTCCAG CTGCTCATCA TGCTCTCGGA AACAACCGCA CTCCCACCCG 
CGTGCCTTTA CCTCCACCTC CTCCTCGTCC TCCATTTCGT CCCCGTCGGC GCAGGACAAG 
AGTGTGTGCC AGTGGACGTG TCCTGCCTGC ACGCTGCTGA ACGAGGGAAG GATGAAGCAC 
TGCGCCGCAT GTCACACGCC GCAGCAGTAC CTGAATCTGA GGAAAACCGG GAAGCCGCTC 
AAGCGCAGGG AGAGCATGCA TGTGGAGGCC CGCAGGCGCA CGGATGAGGG AGAGGCCAAG 
GAATTGTGGG AAAATATCGT CAGCTTCTGT AGGGAGAACG CAGTGAATTT CGTGGATGAC 
AGTTTTCCTC CTGGTCCTCG ATCGGTCGGT TTCCCGGAAA ACGACAGCGT CCAGCAGCGG 
ATCAAGAAGT GGCTCCGTCC TCATGAGATC AACTGCAATA ACTTTAAGGA TCGCGGTGTG 
AAGTGGTCTG TGTTCAGGAC ACCCCGTCCC TCAGATATTC TGCAAGGCCT TCTGGGAAAC 
TGCTGGTTTC TGAGTGCGCT GGCGGTGTTG GCGGAGCGGC CGGAGTTGGT GGAGCGAGTG 
ATGATCACCA GAAGCATCTG TCAGGAAGGA GCCTATCAGG TCCGGCTCTG TAAAGACGGC 
ATGTGGACCA CCGTTTTAGT GGACGACATG CTGCCCTGTG ACGAATACGG CTTTCTGCTC 
TTCTCGCAGG CTCAGCGGAG GCAGCTGTGG GTTGCACTGA TAGAGAAGGC TCTGGCCAAG 
CTGCATGGCT CTTATTTTGC GCTTCAGGCC GGTCGAGCGA TCGAGGGTCT GGCAACGCTC 
ACCGGAGCCC CCTGTGAGAG TCTGATGCTG CAGGTCAGCT CAACAAACCC TCGAGAAGAG 
CCCATCGACA CTGACCTCAT CTGGGCCAAA ATGCTGAGCT CCAAAGAGGC CGGTTTCCTG 
ATGGGTGCGT CCTGTGGGGG CGGGAACATG AAGGTGGATG ATGCTGTGTA TGAATCTCTG 
GGGCTCCGCC CACGACATGC CTACTCCATA CTGGACGTCA GAGACGTGCA GGGCTACAGA 
CTGCTGCGTC TCAGGAACCC GTGGGGCCGG TTCTCCTGGA ACGGCTCCTG GTCGGACGAG 
TGGAGCGACT GGCCGCAGCA CCTCCGGCAC GAGCTCATGG CTCACGGCAG CAGTGAAGGC 
GTCTTCTGGA TGGAATACGG AGACTTCATT AAGTACTTTG ACTCGGTGGA CATCTGTAAG 
ATTCATCCAG ACTGGCAGGA GGTTCGTCTG CAGGGCTGTT TCCCCTGCCG GGCCAGTAAA 
CCGGTGACTG TAACGGCGCT TACGGTGCTG GAGAGAACGG CGCTGGAGTT CGCCCTCTTC 
CAGGAAGGCA GCAGGCGTTC AGACACGGCG GACAGTCACC TGCTGGACTT GTGCATCATG 
GTCTTCCGGG CCTCGTTCAG TAACGGGAAT AAGCTGGTTC TGGGTCGTCT GCTGGCACAC 
AGCAAGCGTG CGGTCAAGAA GTTTGTAGGC TGCGATGTGA TGCTGGAGCC GGGCGAGTAC 
GCCGTGGTTT GCTGTGCCTT CAACCACTGG CAGATGGACC TCAGCGGAGC CTCAACACCT 
GTGTCCAGCC CCACCAATGC TCGCAGACCC AGTCAGGATT TCCCAGGGTA CATTTTGGCC 
ATCTACAGCT CCAGACAGGT GATGGTGGAG CAGGTGGAGG CCACGTCCAC CACACTCGCA 
GACGCCATCA TCCTGCTGAC CGAGAACAAG GGGGAGAGAC ATGAGGGTCG GGAAGGAATG 
ACCTGTTATT ACCTGACCCA CGGGTGGGCG GGGCTTATCG TTGTGGTGGA GAATCGTCAC 
CCAAAGTACT ACCTCCATGT GTCCTGTGAC TGTACAGACA GTTTTAATGT GGTGTCGACA 
CGCGGCAGCC TGAAGACCAT CGACAGCGTT CCTCCTCTGC ACAGGCAGGT TCTGGTGGTT 
CTGTCTCAGC TGGAGGGAAA CGCCGGTTTC TCCATCACAC ACAGACTGGC TCACAGGAAA 
GCAGCACAGG CGTCTTTGGG CGACTGGACA TCCAGTAAAG CCACACACTC GCCCCAGCTC 
ACTCCAGACA CTGACGGACT GCACAGACCA CGGCCGCTTT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

capn15 ( XM_002663847.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_002663893.2 Danio rerio calpain 15 (capn15), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa28888
Clone ID Related Accession (Same CDS sequence)	XM_002663847.6 , XM_002663847.5
Accession Version	XM_002663847.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	calpain-15 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_002663847.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAATT CAGGACCGCA GAAGGGAAAG TTAAAAGGGC AAATCAAGGA AGAAGAGAAG 
CTCGGTCCAT CCAAAGGTCT GCCCGTGCTC CACAATCATT CTCATTCACC TGGTTCGATG 
GAAGCAGTTG TGAGGAATTC TGATTGGTCC TGCGGGCGCT GCACGTTCCT CAACTCCAGC 
GGCTCTATGC GCTGCTCCAT TTGTGAAGCT CCCCGACAGA AGCCGGATCT GAACCACATT 
CTGCGCCTCA GCAGTGCAGA GGAACCCCGC TGGTCCTGCC CACGCTGCAC TCTCACCAAC 
CACCACGGGT TAGGCTCCTG TTCTGTGTGT GGAGCTGCTC CGGTAACACC CAACGGACCC 
CTCCCACCCA AACAAACCCC GCCCACTCCG ATTGACCTCG CCTCTAGCCC TGAACCTAAA 
GGTCAGGTGG CGAATGGGGA ATCCCAGTGC ATAGAGTCCA ATGGGGAGGT GCCTATGAGT 
GGGCAGGGCT CCTCAGAGTG GGCGTGTCCT CGTTGTACTT TAGTGAATAC TCCAGTTGCG 
TTGTCGTGTT CAGCGTGTGG TGGTCCGAGG AAACTTTCCT TACCGAAAAT CCCTCCGGAG 
GCTCTAGTGG TACCAGAAGT GCGCACACCT GTGGCAGGGT TTCCTACGCA AACGGCAGGA 
GCATCTGCAC CTCTTCTTAT CGATCTAACC GAAGATTCTC CACCGCAGGC ACCGTCTGAA 
ACCCCATCTC CACCCCCTGT CCCGTTTCTG CCCCCTTCGT TGTCCTCACT TCAAAATAAT 
CCTGTGCCTC GCAGCCGAAG AGAAGTCCCG CCCCCTGGGC GGCCTCCAAA CCCTGGCCCC 
TCACCAACCT CACCTTCATC CTGCCCACCA ACCAAGCCTA AACCCCTCCC ACCATCAGGT 
AATTACCCAG TCAAGCGCCT CAGCGTGCTG GAGGAGGAGG AGCCTAATAA CCCCACCCCT 
CCAGTCACAT CTCCGCCTTC CTGGAAGTGC CCCGGATGCT CCGCCCCAAC AGCTCCTCCT 
TCATCGTCTG CCGGGCGCTG CGATGCATGC AGAGGGTCGA GGCCGGGTTC GGGTGGTGAT 
GTCATCGATG TGCTCGGCGA ATCAGTGCGT TTCACGCCAG CGTCGCCATC CAGCCCAGAC 
TTCAGCTCCT GGGCGTGCTC CAAATGTACG CTGCGCAACC CGACGGGCGC GGGTCACTGT 
ACGGCCTGCG GATCCTCCAA ACTGCATGGT TTCTCGGAGC CGTCCAGCTG CTCATCATGC 
TCTCGGAAAC AACCGCACTC CCACCCGCGT GCCTTTACCT CCACCTCCTC CTCGTCCTCC 
ATTTCGTCCC CGTCGGCGCA GGACAAGAGT GTGTGCCAGT GGACGTGTCC TGCCTGCACG 
CTGCTGAACG AGGGAAGGAT GAAGCACTGC GCCGCATGTC ACACGCCGCA GCAGTACCTG 
AATCTGAGGA AAACCGGGAA GCCGCTCAAG CGCAGGGAGA GCATGCATGT GGAGGCCCGC 
AGGCGCACGG ATGAGGGAGA GGCCAAGGAA TTGTGGGAAA ATATCGTCAG CTTCTGTAGG 
GAGAACGCAG TGAATTTCGT GGATGACAGT TTTCCTCCTG GTCCTCGATC GGTCGGTTTC 
CCGGAAAACG ACAGCGTCCA GCAGCGGATC AAGAAGTGGC TCCGTCCTCA TGAGATCAAC 
TGCAATAACT TTAAGGATCG CGGTGTGAAG TGGTCTGTGT TCAGGACACC CCGTCCCTCA 
GATATTCTGC AAGGCCTTCT GGGAAACTGC TGGTTTCTGA GTGCGCTGGC GGTGTTGGCG 
GAGCGGCCGG AGTTGGTGGA GCGAGTGATG ATCACCAGAA GCATCTGTCA GGAAGGAGCC 
TATCAGGTCC GGCTCTGTAA AGACGGCATG TGGACCACCG TTTTAGTGGA CGACATGCTG 
CCCTGTGACG AATACGGCTT TCTGCTCTTC TCGCAGGCTC AGCGGAGGCA GCTGTGGGTT 
GCACTGATAG AGAAGGCTCT GGCCAAGCTG CATGGCTCTT ATTTTGCGCT TCAGGCCGGT 
CGAGCGATCG AGGGTCTGGC AACGCTCACC GGAGCCCCCT GTGAGAGTCT GATGCTGCAG 
GTCAGCTCAA CAAACCCTCG AGAAGAGCCC ATCGACACTG ACCTCATCTG GGCCAAAATG 
CTGAGCTCCA AAGAGGCCGG TTTCCTGATG GGTGCGTCCT GTGGGGGCGG GAACATGAAG 
GTGGATGATG CTGTGTATGA ATCTCTGGGG CTCCGCCCAC GACATGCCTA CTCCATACTG 
GACGTCAGAG ACGTGCAGGG CTACAGACTG CTGCGTCTCA GGAACCCGTG GGGCCGGTTC 
TCCTGGAACG GCTCCTGGTC GGACGAGTGG AGCGACTGGC CGCAGCACCT CCGGCACGAG 
CTCATGGCTC ACGGCAGCAG TGAAGGCGTC TTCTGGATGG AATACGGAGA CTTCATTAAG 
TACTTTGACT CGGTGGACAT CTGTAAGATT CATCCAGACT GGCAGGAGGT TCGTCTGCAG 
GGCTGTTTCC CCTGCCGGGC CAGTAAACCG GTGACTGTAA CGGCGCTTAC GGTGCTGGAG 
AGAACGGCGC TGGAGTTCGC CCTCTTCCAG GAAGGCAGCA GGCGTTCAGA CACGGCGGAC 
AGTCACCTGC TGGACTTGTG CATCATGGTC TTCCGGGCCT CGTTCAGTAA CGGGAATAAG 
CTGGTTCTGG GTCGTCTGCT GGCACACAGC AAGCGTGCGG TCAAGAAGTT TGTAGGCTGC 
GATGTGATGC TGGAGCCGGG CGAGTACGCC GTGGTTTGCT GTGCCTTCAA CCACTGGCAG 
ATGGACCTCA GCGGAGCCTC AACACCTGTG TCCAGCCCCA CCAATGCTCG CAGACCCAGT 
CAGGATTTCC CAGGGTACAT TTTGGCCATC TACAGCTCCA GACAGGTGAT GGTGGAGCAG 
GTGGAGGCCA CGTCCACCAC ACTCGCAGAC GCCATCATCC TGCTGACCGA GAACAAGGGG 
GAGAGACATG AGGGTCGGGA AGGAATGACC TGTTATTACC TGACCCACGG GTGGGCGGGG 
CTTATCGTTG TGGTGGAGAA TCGTCACCCA AAGTACTACC TCCATGTGTC CTGTGACTGT 
ACAGACAGTT TTAATGTGGT GTCGACACGC GGCAGCCTGA AGACCATCGA CAGCGTTCCT 
CCTCTGCACA GGCAGGTTCT GGTGGTTCTG TCTCAGCTGG AGGGAAACGC CGGTTTCTCC 
ATCACACACA GACTGGCTCA CAGGAAAGCA GCACAGGCGT CTTTGGGCGA CTGGACATCC 
AGTAAAGCCA CACACTCGCC CCAGCTCACT CCAGACACTG ACGGACTGCA CAGACCACGG 
CCGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002663893.2
CDS	192..3560
Translation	MANSGPQKGKLKGQIKEEEKLGPSKGLPVLHNHSHSPGSMEAVVRNSDWSCGRCTFLNSSGSMRCSICEAPRQKPDLNHILRLSSAEEPRWSCPRCTLTNHHGLGSCSVCGAAPVTPNGPLPPKQTPPTPIDLASSPEPKGQVANGESQCIESNGEVPMSGQGSSEWACPRCTLVNTPVALSCSACGGPRKLSLPKIPPEALVVPEVRTPVAGFPTQTAGASAPLLIDLTEDSPPQAPSETPSPPPVPFLPPSLSSLQNNPVPRSRREVPPPGRPPNPGPSPTSPSSCPPTKPKPLPPSGNYPVKRLSVLEEEEPNNPTPPVTSPPSWKCPGCSAPTAPPSSSAGRCDACRGSRPGSGGDVIDVLGESVRFTPASPSSPDFSSWACSKCTLRNPTGAGHCTACGSSKLHGFSEPSSCSSCSRKQPHSHPRAFTSTSSSSSISSPSAQDKSVCQWTCPACTLLNEGRMKHCAACHTPQQYLNLRKTGKPLKRRESMHVEARRRTDEGEAKELWENIVSFCRENAVNFVDDSFPPGPRSVGFPENDSVQQRIKKWLRPHEINCNNFKDRGVKWSVFRTPRPSDILQGLLGNCWFLSALAVLAERPELVERVMITRSICQEGAYQVRLCKDGMWTTVLVDDMLPCDEYGFLLFSQAQRRQLWVALIEKALAKLHGSYFALQAGRAIEGLATLTGAPCESLMLQVSSTNPREEPIDTDLIWAKMLSSKEAGFLMGASCGGGNMKVDDAVYESLGLRPRHAYSILDVRDVQGYRLLRLRNPWGRFSWNGSWSDEWSDWPQHLRHELMAHGSSEGVFWMEYGDFIKYFDSVDICKIHPDWQEVRLQGCFPCRASKPVTVTALTVLERTALEFALFQEGSRRSDTADSHLLDLCIMVFRASFSNGNKLVLGRLLAHSKRAVKKFVGCDVMLEPGEYAVVCCAFNHWQMDLSGASTPVSSPTNARRPSQDFPGYILAIYSSRQVMVEQVEATSTTLADAIILLTENKGERHEGREGMTCYYLTHGWAGLIVVVENRHPKYYLHVSCDCTDSFNVVSTRGSLKTIDSVPPLHRQVLVVLSQLEGNAGFSITHRLAHRKAAQASLGDWTSSKATHSPQLTPDTDGLHRPRPL

Target ORF information:

RefSeq Version	XM_002663847.6
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calpain 15 (capn15), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002663847.6

ORF Insert Sequence:

ATGGCTAATT CAGGACCGCA GAAGGGAAAG TTAAAAGGGC AAATCAAGGA AGAAGAGAAG 
CTCGGTCCAT CCAAAGGTCT GCCCGTGCTC CACAATCATT CTCATTCACC TGGTTCGATG 
GAAGCAGTTG TGAGGAATTC TGATTGGTCC TGCGGGCGCT GCACGTTCCT CAACTCCAGC 
GGCTCTATGC GCTGCTCCAT TTGTGAAGCT CCCCGACAGA AGCCGGATCT GAACCACATT 
CTGCGCCTCA GCAGTGCAGA GGAACCCCGC TGGTCCTGCC CACGCTGCAC TCTCACCAAC 
CACCACGGGT TAGGCTCCTG TTCTGTGTGT GGAGCTGCTC CGGTAACACC CAACGGACCC 
CTCCCACCCA AACAAACCCC GCCCACTCCG ATTGACCTCG CCTCTAGCCC TGAACCTAAA 
GGTCAGGTGG CGAATGGGGA ATCCCAGTGC ATAGAGTCCA ATGGGGAGGT GCCTATGAGT 
GGGCAGGGCT CCTCAGAGTG GGCGTGTCCT CGTTGTACTT TAGTGAATAC TCCAGTTGCG 
TTGTCGTGTT CAGCGTGTGG TGGTCCGAGG AAACTTTCCT TACCGAAAAT CCCTCCGGAG 
GCTCTAGTGG TACCAGAAGT GCGCACACCT GTGGCAGGGT TTCCTACGCA AACGGCAGGA 
GCATCTGCAC CTCTTCTTAT CGATCTAACC GAAGATTCTC CACCGCAGGC ACCGTCTGAA 
ACCCCATCTC CACCCCCTGT CCCGTTTCTG CCCCCTTCGT TGTCCTCACT TCAAAATAAT 
CCTGTGCCTC GCAGCCGAAG AGAAGTCCCG CCCCCTGGGC GGCCTCCAAA CCCTGGCCCC 
TCACCAACCT CACCTTCATC CTGCCCACCA ACCAAGCCTA AACCCCTCCC ACCATCAGGT 
AATTACCCAG TCAAGCGCCT CAGCGTGCTG GAGGAGGAGG AGCCTAATAA CCCCACCCCT 
CCAGTCACAT CTCCGCCTTC CTGGAAGTGC CCCGGATGCT CCGCCCCAAC AGCTCCTCCT 
TCATCGTCTG CCGGGCGCTG CGATGCATGC AGAGGGTCGA GGCCGGGTTC GGGTGGTGAT 
GTCATCGATG TGCTCGGCGA ATCAGTGCGT TTCACGCCAG CGTCGCCATC CAGCCCAGAC 
TTCAGCTCCT GGGCGTGCTC CAAATGTACG CTGCGCAACC CGACGGGCGC GGGTCACTGT 
ACGGCCTGCG GATCCTCCAA ACTGCATGGT TTCTCGGAGC CGTCCAGCTG CTCATCATGC 
TCTCGGAAAC AACCGCACTC CCACCCGCGT GCCTTTACCT CCACCTCCTC CTCGTCCTCC 
ATTTCGTCCC CGTCGGCGCA GGACAAGAGT GTGTGCCAGT GGACGTGTCC TGCCTGCACG 
CTGCTGAACG AGGGAAGGAT GAAGCACTGC GCCGCATGTC ACACGCCGCA GCAGTACCTG 
AATCTGAGGA AAACCGGGAA GCCGCTCAAG CGCAGGGAGA GCATGCATGT GGAGGCCCGC 
AGGCGCACGG ATGAGGGAGA GGCCAAGGAA TTGTGGGAAA ATATCGTCAG CTTCTGTAGG 
GAGAACGCAG TGAATTTCGT GGATGACAGT TTTCCTCCTG GTCCTCGATC GGTCGGTTTC 
CCGGAAAACG ACAGCGTCCA GCAGCGGATC AAGAAGTGGC TCCGTCCTCA TGAGATCAAC 
TGCAATAACT TTAAGGATCG CGGTGTGAAG TGGTCTGTGT TCAGGACACC CCGTCCCTCA 
GATATTCTGC AAGGCCTTCT GGGAAACTGC TGGTTTCTGA GTGCGCTGGC GGTGTTGGCG 
GAGCGGCCGG AGTTGGTGGA GCGAGTGATG ATCACCAGAA GCATCTGTCA GGAAGGAGCC 
TATCAGGTCC GGCTCTGTAA AGACGGCATG TGGACCACCG TTTTAGTGGA CGACATGCTG 
CCCTGTGACG AATACGGCTT TCTGCTCTTC TCGCAGGCTC AGCGGAGGCA GCTGTGGGTT 
GCACTGATAG AGAAGGCTCT GGCCAAGCTG CATGGCTCTT ATTTTGCGCT TCAGGCCGGT 
CGAGCGATCG AGGGTCTGGC AACGCTCACC GGAGCCCCCT GTGAGAGTCT GATGCTGCAG 
GTCAGCTCAA CAAACCCTCG AGAAGAGCCC ATCGACACTG ACCTCATCTG GGCCAAAATG 
CTGAGCTCCA AAGAGGCCGG TTTCCTGATG GGTGCGTCCT GTGGGGGCGG GAACATGAAG 
GTGGATGATG CTGTGTATGA ATCTCTGGGG CTCCGCCCAC GACATGCCTA CTCCATACTG 
GACGTCAGAG ACGTGCAGGG CTACAGACTG CTGCGTCTCA GGAACCCGTG GGGCCGGTTC 
TCCTGGAACG GCTCCTGGTC GGACGAGTGG AGCGACTGGC CGCAGCACCT CCGGCACGAG 
CTCATGGCTC ACGGCAGCAG TGAAGGCGTC TTCTGGATGG AATACGGAGA CTTCATTAAG 
TACTTTGACT CGGTGGACAT CTGTAAGATT CATCCAGACT GGCAGGAGGT TCGTCTGCAG 
GGCTGTTTCC CCTGCCGGGC CAGTAAACCG GTGACTGTAA CGGCGCTTAC GGTGCTGGAG 
AGAACGGCGC TGGAGTTCGC CCTCTTCCAG GAAGGCAGCA GGCGTTCAGA CACGGCGGAC 
AGTCACCTGC TGGACTTGTG CATCATGGTC TTCCGGGCCT CGTTCAGTAA CGGGAATAAG 
CTGGTTCTGG GTCGTCTGCT GGCACACAGC AAGCGTGCGG TCAAGAAGTT TGTAGGCTGC 
GATGTGATGC TGGAGCCGGG CGAGTACGCC GTGGTTTGCT GTGCCTTCAA CCACTGGCAG 
ATGGACCTCA GCGGAGCCTC AACACCTGTG TCCAGCCCCA CCAATGCTCG CAGACCCAGT 
CAGGATTTCC CAGGGTACAT TTTGGCCATC TACAGCTCCA GACAGGTGAT GGTGGAGCAG 
GTGGAGGCCA CGTCCACCAC ACTCGCAGAC GCCATCATCC TGCTGACCGA GAACAAGGGG 
GAGAGACATG AGGGTCGGGA AGGAATGACC TGTTATTACC TGACCCACGG GTGGGCGGGG 
CTTATCGTTG TGGTGGAGAA TCGTCACCCA AAGTACTACC TCCATGTGTC CTGTGACTGT 
ACAGACAGTT TTAATGTGGT GTCGACACGC GGCAGCCTGA AGACCATCGA CAGCGTTCCT 
CCTCTGCACA GGCAGGTTCT GGTGGTTCTG TCTCAGCTGG AGGGAAACGC CGGTTTCTCC 
ATCACACACA GACTGGCTCA CAGGAAAGCA GCACAGGCGT CTTTGGGCGA CTGGACATCC 
AGTAAAGCCA CACACTCGCC CCAGCTCACT CCAGACACTG ACGGACTGCA CAGACCACGG 
CCGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

capn15 ( XM_002663847.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_002663893.2 Danio rerio calpain 15 (capn15), transcript variant X2, mRNA.

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CloneID	ODa28888
Clone ID Related Accession (Same CDS sequence)	XM_002663847.6 , XM_002663847.5
Accession Version	XM_002663847.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3369bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	calpain-15 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_002663847.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCTAATT CAGGACCGCA GAAGGGAAAG TTAAAAGGGC AAATCAAGGA AGAAGAGAAG 
CTCGGTCCAT CCAAAGGTCT GCCCGTGCTC CACAATCATT CTCATTCACC TGGTTCGATG 
GAAGCAGTTG TGAGGAATTC TGATTGGTCC TGCGGGCGCT GCACGTTCCT CAACTCCAGC 
GGCTCTATGC GCTGCTCCAT TTGTGAAGCT CCCCGACAGA AGCCGGATCT GAACCACATT 
CTGCGCCTCA GCAGTGCAGA GGAACCCCGC TGGTCCTGCC CACGCTGCAC TCTCACCAAC 
CACCACGGGT TAGGCTCCTG TTCTGTGTGT GGAGCTGCTC CGGTAACACC CAACGGACCC 
CTCCCACCCA AACAAACCCC GCCCACTCCG ATTGACCTCG CCTCTAGCCC TGAACCTAAA 
GGTCAGGTGG CGAATGGGGA ATCCCAGTGC ATAGAGTCCA ATGGGGAGGT GCCTATGAGT 
GGGCAGGGCT CCTCAGAGTG GGCGTGTCCT CGTTGTACTT TAGTGAATAC TCCAGTTGCG 
TTGTCGTGTT CAGCGTGTGG TGGTCCGAGG AAACTTTCCT TACCGAAAAT CCCTCCGGAG 
GCTCTAGTGG TACCAGAAGT GCGCACACCT GTGGCAGGGT TTCCTACGCA AACGGCAGGA 
GCATCTGCAC CTCTTCTTAT CGATCTAACC GAAGATTCTC CACCGCAGGC ACCGTCTGAA 
ACCCCATCTC CACCCCCTGT CCCGTTTCTG CCCCCTTCGT TGTCCTCACT TCAAAATAAT 
CCTGTGCCTC GCAGCCGAAG AGAAGTCCCG CCCCCTGGGC GGCCTCCAAA CCCTGGCCCC 
TCACCAACCT CACCTTCATC CTGCCCACCA ACCAAGCCTA AACCCCTCCC ACCATCAGGT 
AATTACCCAG TCAAGCGCCT CAGCGTGCTG GAGGAGGAGG AGCCTAATAA CCCCACCCCT 
CCAGTCACAT CTCCGCCTTC CTGGAAGTGC CCCGGATGCT CCGCCCCAAC AGCTCCTCCT 
TCATCGTCTG CCGGGCGCTG CGATGCATGC AGAGGGTCGA GGCCGGGTTC GGGTGGTGAT 
GTCATCGATG TGCTCGGCGA ATCAGTGCGT TTCACGCCAG CGTCGCCATC CAGCCCAGAC 
TTCAGCTCCT GGGCGTGCTC CAAATGTACG CTGCGCAACC CGACGGGCGC GGGTCACTGT 
ACGGCCTGCG GATCCTCCAA ACTGCATGGT TTCTCGGAGC CGTCCAGCTG CTCATCATGC 
TCTCGGAAAC AACCGCACTC CCACCCGCGT GCCTTTACCT CCACCTCCTC CTCGTCCTCC 
ATTTCGTCCC CGTCGGCGCA GGACAAGAGT GTGTGCCAGT GGACGTGTCC TGCCTGCACG 
CTGCTGAACG AGGGAAGGAT GAAGCACTGC GCCGCATGTC ACACGCCGCA GCAGTACCTG 
AATCTGAGGA AAACCGGGAA GCCGCTCAAG CGCAGGGAGA GCATGCATGT GGAGGCCCGC 
AGGCGCACGG ATGAGGGAGA GGCCAAGGAA TTGTGGGAAA ATATCGTCAG CTTCTGTAGG 
GAGAACGCAG TGAATTTCGT GGATGACAGT TTTCCTCCTG GTCCTCGATC GGTCGGTTTC 
CCGGAAAACG ACAGCGTCCA GCAGCGGATC AAGAAGTGGC TCCGTCCTCA TGAGATCAAC 
TGCAATAACT TTAAGGATCG CGGTGTGAAG TGGTCTGTGT TCAGGACACC CCGTCCCTCA 
GATATTCTGC AAGGCCTTCT GGGAAACTGC TGGTTTCTGA GTGCGCTGGC GGTGTTGGCG 
GAGCGGCCGG AGTTGGTGGA GCGAGTGATG ATCACCAGAA GCATCTGTCA GGAAGGAGCC 
TATCAGGTCC GGCTCTGTAA AGACGGCATG TGGACCACCG TTTTAGTGGA CGACATGCTG 
CCCTGTGACG AATACGGCTT TCTGCTCTTC TCGCAGGCTC AGCGGAGGCA GCTGTGGGTT 
GCACTGATAG AGAAGGCTCT GGCCAAGCTG CATGGCTCTT ATTTTGCGCT TCAGGCCGGT 
CGAGCGATCG AGGGTCTGGC AACGCTCACC GGAGCCCCCT GTGAGAGTCT GATGCTGCAG 
GTCAGCTCAA CAAACCCTCG AGAAGAGCCC ATCGACACTG ACCTCATCTG GGCCAAAATG 
CTGAGCTCCA AAGAGGCCGG TTTCCTGATG GGTGCGTCCT GTGGGGGCGG GAACATGAAG 
GTGGATGATG CTGTGTATGA ATCTCTGGGG CTCCGCCCAC GACATGCCTA CTCCATACTG 
GACGTCAGAG ACGTGCAGGG CTACAGACTG CTGCGTCTCA GGAACCCGTG GGGCCGGTTC 
TCCTGGAACG GCTCCTGGTC GGACGAGTGG AGCGACTGGC CGCAGCACCT CCGGCACGAG 
CTCATGGCTC ACGGCAGCAG TGAAGGCGTC TTCTGGATGG AATACGGAGA CTTCATTAAG 
TACTTTGACT CGGTGGACAT CTGTAAGATT CATCCAGACT GGCAGGAGGT TCGTCTGCAG 
GGCTGTTTCC CCTGCCGGGC CAGTAAACCG GTGACTGTAA CGGCGCTTAC GGTGCTGGAG 
AGAACGGCGC TGGAGTTCGC CCTCTTCCAG GAAGGCAGCA GGCGTTCAGA CACGGCGGAC 
AGTCACCTGC TGGACTTGTG CATCATGGTC TTCCGGGCCT CGTTCAGTAA CGGGAATAAG 
CTGGTTCTGG GTCGTCTGCT GGCACACAGC AAGCGTGCGG TCAAGAAGTT TGTAGGCTGC 
GATGTGATGC TGGAGCCGGG CGAGTACGCC GTGGTTTGCT GTGCCTTCAA CCACTGGCAG 
ATGGACCTCA GCGGAGCCTC AACACCTGTG TCCAGCCCCA CCAATGCTCG CAGACCCAGT 
CAGGATTTCC CAGGGTACAT TTTGGCCATC TACAGCTCCA GACAGGTGAT GGTGGAGCAG 
GTGGAGGCCA CGTCCACCAC ACTCGCAGAC GCCATCATCC TGCTGACCGA GAACAAGGGG 
GAGAGACATG AGGGTCGGGA AGGAATGACC TGTTATTACC TGACCCACGG GTGGGCGGGG 
CTTATCGTTG TGGTGGAGAA TCGTCACCCA AAGTACTACC TCCATGTGTC CTGTGACTGT 
ACAGACAGTT TTAATGTGGT GTCGACACGC GGCAGCCTGA AGACCATCGA CAGCGTTCCT 
CCTCTGCACA GGCAGGTTCT GGTGGTTCTG TCTCAGCTGG AGGGAAACGC CGGTTTCTCC 
ATCACACACA GACTGGCTCA CAGGAAAGCA GCACAGGCGT CTTTGGGCGA CTGGACATCC 
AGTAAAGCCA CACACTCGCC CCAGCTCACT CCAGACACTG ACGGACTGCA CAGACCACGG 
CCGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002663893.2
CDS	203..3571
Translation	MANSGPQKGKLKGQIKEEEKLGPSKGLPVLHNHSHSPGSMEAVVRNSDWSCGRCTFLNSSGSMRCSICEAPRQKPDLNHILRLSSAEEPRWSCPRCTLTNHHGLGSCSVCGAAPVTPNGPLPPKQTPPTPIDLASSPEPKGQVANGESQCIESNGEVPMSGQGSSEWACPRCTLVNTPVALSCSACGGPRKLSLPKIPPEALVVPEVRTPVAGFPTQTAGASAPLLIDLTEDSPPQAPSETPSPPPVPFLPPSLSSLQNNPVPRSRREVPPPGRPPNPGPSPTSPSSCPPTKPKPLPPSGNYPVKRLSVLEEEEPNNPTPPVTSPPSWKCPGCSAPTAPPSSSAGRCDACRGSRPGSGGDVIDVLGESVRFTPASPSSPDFSSWACSKCTLRNPTGAGHCTACGSSKLHGFSEPSSCSSCSRKQPHSHPRAFTSTSSSSSISSPSAQDKSVCQWTCPACTLLNEGRMKHCAACHTPQQYLNLRKTGKPLKRRESMHVEARRRTDEGEAKELWENIVSFCRENAVNFVDDSFPPGPRSVGFPENDSVQQRIKKWLRPHEINCNNFKDRGVKWSVFRTPRPSDILQGLLGNCWFLSALAVLAERPELVERVMITRSICQEGAYQVRLCKDGMWTTVLVDDMLPCDEYGFLLFSQAQRRQLWVALIEKALAKLHGSYFALQAGRAIEGLATLTGAPCESLMLQVSSTNPREEPIDTDLIWAKMLSSKEAGFLMGASCGGGNMKVDDAVYESLGLRPRHAYSILDVRDVQGYRLLRLRNPWGRFSWNGSWSDEWSDWPQHLRHELMAHGSSEGVFWMEYGDFIKYFDSVDICKIHPDWQEVRLQGCFPCRASKPVTVTALTVLERTALEFALFQEGSRRSDTADSHLLDLCIMVFRASFSNGNKLVLGRLLAHSKRAVKKFVGCDVMLEPGEYAVVCCAFNHWQMDLSGASTPVSSPTNARRPSQDFPGYILAIYSSRQVMVEQVEATSTTLADAIILLTENKGERHEGREGMTCYYLTHGWAGLIVVVENRHPKYYLHVSCDCTDSFNVVSTRGSLKTIDSVPPLHRQVLVVLSQLEGNAGFSITHRLAHRKAAQASLGDWTSSKATHSPQLTPDTDGLHRPRPL

Target ORF information:

RefSeq Version	XM_002663847.5
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calpain 15 (capn15), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002663847.5

ORF Insert Sequence:

ATGGCTAATT CAGGACCGCA GAAGGGAAAG TTAAAAGGGC AAATCAAGGA AGAAGAGAAG 
CTCGGTCCAT CCAAAGGTCT GCCCGTGCTC CACAATCATT CTCATTCACC TGGTTCGATG 
GAAGCAGTTG TGAGGAATTC TGATTGGTCC TGCGGGCGCT GCACGTTCCT CAACTCCAGC 
GGCTCTATGC GCTGCTCCAT TTGTGAAGCT CCCCGACAGA AGCCGGATCT GAACCACATT 
CTGCGCCTCA GCAGTGCAGA GGAACCCCGC TGGTCCTGCC CACGCTGCAC TCTCACCAAC 
CACCACGGGT TAGGCTCCTG TTCTGTGTGT GGAGCTGCTC CGGTAACACC CAACGGACCC 
CTCCCACCCA AACAAACCCC GCCCACTCCG ATTGACCTCG CCTCTAGCCC TGAACCTAAA 
GGTCAGGTGG CGAATGGGGA ATCCCAGTGC ATAGAGTCCA ATGGGGAGGT GCCTATGAGT 
GGGCAGGGCT CCTCAGAGTG GGCGTGTCCT CGTTGTACTT TAGTGAATAC TCCAGTTGCG 
TTGTCGTGTT CAGCGTGTGG TGGTCCGAGG AAACTTTCCT TACCGAAAAT CCCTCCGGAG 
GCTCTAGTGG TACCAGAAGT GCGCACACCT GTGGCAGGGT TTCCTACGCA AACGGCAGGA 
GCATCTGCAC CTCTTCTTAT CGATCTAACC GAAGATTCTC CACCGCAGGC ACCGTCTGAA 
ACCCCATCTC CACCCCCTGT CCCGTTTCTG CCCCCTTCGT TGTCCTCACT TCAAAATAAT 
CCTGTGCCTC GCAGCCGAAG AGAAGTCCCG CCCCCTGGGC GGCCTCCAAA CCCTGGCCCC 
TCACCAACCT CACCTTCATC CTGCCCACCA ACCAAGCCTA AACCCCTCCC ACCATCAGGT 
AATTACCCAG TCAAGCGCCT CAGCGTGCTG GAGGAGGAGG AGCCTAATAA CCCCACCCCT 
CCAGTCACAT CTCCGCCTTC CTGGAAGTGC CCCGGATGCT CCGCCCCAAC AGCTCCTCCT 
TCATCGTCTG CCGGGCGCTG CGATGCATGC AGAGGGTCGA GGCCGGGTTC GGGTGGTGAT 
GTCATCGATG TGCTCGGCGA ATCAGTGCGT TTCACGCCAG CGTCGCCATC CAGCCCAGAC 
TTCAGCTCCT GGGCGTGCTC CAAATGTACG CTGCGCAACC CGACGGGCGC GGGTCACTGT 
ACGGCCTGCG GATCCTCCAA ACTGCATGGT TTCTCGGAGC CGTCCAGCTG CTCATCATGC 
TCTCGGAAAC AACCGCACTC CCACCCGCGT GCCTTTACCT CCACCTCCTC CTCGTCCTCC 
ATTTCGTCCC CGTCGGCGCA GGACAAGAGT GTGTGCCAGT GGACGTGTCC TGCCTGCACG 
CTGCTGAACG AGGGAAGGAT GAAGCACTGC GCCGCATGTC ACACGCCGCA GCAGTACCTG 
AATCTGAGGA AAACCGGGAA GCCGCTCAAG CGCAGGGAGA GCATGCATGT GGAGGCCCGC 
AGGCGCACGG ATGAGGGAGA GGCCAAGGAA TTGTGGGAAA ATATCGTCAG CTTCTGTAGG 
GAGAACGCAG TGAATTTCGT GGATGACAGT TTTCCTCCTG GTCCTCGATC GGTCGGTTTC 
CCGGAAAACG ACAGCGTCCA GCAGCGGATC AAGAAGTGGC TCCGTCCTCA TGAGATCAAC 
TGCAATAACT TTAAGGATCG CGGTGTGAAG TGGTCTGTGT TCAGGACACC CCGTCCCTCA 
GATATTCTGC AAGGCCTTCT GGGAAACTGC TGGTTTCTGA GTGCGCTGGC GGTGTTGGCG 
GAGCGGCCGG AGTTGGTGGA GCGAGTGATG ATCACCAGAA GCATCTGTCA GGAAGGAGCC 
TATCAGGTCC GGCTCTGTAA AGACGGCATG TGGACCACCG TTTTAGTGGA CGACATGCTG 
CCCTGTGACG AATACGGCTT TCTGCTCTTC TCGCAGGCTC AGCGGAGGCA GCTGTGGGTT 
GCACTGATAG AGAAGGCTCT GGCCAAGCTG CATGGCTCTT ATTTTGCGCT TCAGGCCGGT 
CGAGCGATCG AGGGTCTGGC AACGCTCACC GGAGCCCCCT GTGAGAGTCT GATGCTGCAG 
GTCAGCTCAA CAAACCCTCG AGAAGAGCCC ATCGACACTG ACCTCATCTG GGCCAAAATG 
CTGAGCTCCA AAGAGGCCGG TTTCCTGATG GGTGCGTCCT GTGGGGGCGG GAACATGAAG 
GTGGATGATG CTGTGTATGA ATCTCTGGGG CTCCGCCCAC GACATGCCTA CTCCATACTG 
GACGTCAGAG ACGTGCAGGG CTACAGACTG CTGCGTCTCA GGAACCCGTG GGGCCGGTTC 
TCCTGGAACG GCTCCTGGTC GGACGAGTGG AGCGACTGGC CGCAGCACCT CCGGCACGAG 
CTCATGGCTC ACGGCAGCAG TGAAGGCGTC TTCTGGATGG AATACGGAGA CTTCATTAAG 
TACTTTGACT CGGTGGACAT CTGTAAGATT CATCCAGACT GGCAGGAGGT TCGTCTGCAG 
GGCTGTTTCC CCTGCCGGGC CAGTAAACCG GTGACTGTAA CGGCGCTTAC GGTGCTGGAG 
AGAACGGCGC TGGAGTTCGC CCTCTTCCAG GAAGGCAGCA GGCGTTCAGA CACGGCGGAC 
AGTCACCTGC TGGACTTGTG CATCATGGTC TTCCGGGCCT CGTTCAGTAA CGGGAATAAG 
CTGGTTCTGG GTCGTCTGCT GGCACACAGC AAGCGTGCGG TCAAGAAGTT TGTAGGCTGC 
GATGTGATGC TGGAGCCGGG CGAGTACGCC GTGGTTTGCT GTGCCTTCAA CCACTGGCAG 
ATGGACCTCA GCGGAGCCTC AACACCTGTG TCCAGCCCCA CCAATGCTCG CAGACCCAGT 
CAGGATTTCC CAGGGTACAT TTTGGCCATC TACAGCTCCA GACAGGTGAT GGTGGAGCAG 
GTGGAGGCCA CGTCCACCAC ACTCGCAGAC GCCATCATCC TGCTGACCGA GAACAAGGGG 
GAGAGACATG AGGGTCGGGA AGGAATGACC TGTTATTACC TGACCCACGG GTGGGCGGGG 
CTTATCGTTG TGGTGGAGAA TCGTCACCCA AAGTACTACC TCCATGTGTC CTGTGACTGT 
ACAGACAGTT TTAATGTGGT GTCGACACGC GGCAGCCTGA AGACCATCGA CAGCGTTCCT 
CCTCTGCACA GGCAGGTTCT GGTGGTTCTG TCTCAGCTGG AGGGAAACGC CGGTTTCTCC 
ATCACACACA GACTGGCTCA CAGGAAAGCA GCACAGGCGT CTTTGGGCGA CTGGACATCC 
AGTAAAGCCA CACACTCGCC CCAGCTCACT CCAGACACTG ACGGACTGCA CAGACCACGG 
CCGCTTTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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capn15 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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見積依頼

Eメール

電話

FAX

オンラインフォーム

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