irs4a cDNA ORF clone, Danio rerio(zebrafish)

The following irs4a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the irs4a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa26239 XM_002666075.6
Latest version!
Danio rerio insulin receptor substrate 4a (irs4a), mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $629.30
$899.00
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ODa26239 XM_002666075.5 Danio rerio insulin receptor substrate 4a (irs4a), mRNA. pcDNA3.1-C-(k)DYK or customized vector 15-17 $629.30
$899.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa26239
    Clone ID Related Accession (Same CDS sequence) XM_002666075.5 , XM_002666075.6
    Accession Version XM_002666075.5 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3156bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product insulin receptor substrate 4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_002666075.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGAAGATC GGCCGCGTTT ACCGAATCAC GGCGTCATGT TGACACTGGA GGCTCAACCG 
    CGTCGCGCTG TCACGAAAAC ATCCGCGAGC GGCATCTCTT GTGCTGCTGC CGGGGAGCCT
    CCATCCTCCT CCTCCTCCTC CTCCTCCTCC TCCACCTCCA CCTCAATCAA TAACAGCGGC
    TGCCGTTTGC CCATGCACTT ATCAGCCTCC TCTCATTGCC AGCCACAGAG GAGGTACCAT
    CCGCCTTACC ACTTTAAAGT GCACCATCTG TTCCCGGATG AGAGCCCTCA GGATGCTGTG
    CTGAGGAAGA ACCTGCCAGC CCTACAGTAT AAGGTGCACG ACTCTGCCTT CTGCCGCGTG
    CTCAGCGCTG ACACCACTGC TGCCGCCCTG GCCGCTGCCG CCGCCTGCTC TGGTGGCCTG
    GACCGGGACA TCTGGAAGTG TGGCTATCTG CGAAAGCAGA AGCACGGCCA CAAGAGGTTC
    TTCGTATTGC GGGGCCCCAG CAACCTCGGG CCCAGCCGCT TGGAGTATTA CGACAGCGAG
    AAAAAATTTC GCAATGCCCT CAAAGCTGCT GCTTCTGGTG TCACGTGCCC TGCCAAAAGG
    GTGATTTATC TGTCGCAGTG TTTTACGGTT AACAAGAGGG CTGATGCTAA GAACAAATAC
    CTCATTGCCC TTTATACTAA AGACGAATAC TTTGCTGTTG TAGCCGAGAG CGAACAGGAG
    CAAGAAGAGT GGTACTTGTC TATAACGGAA CTTATGACGG AGGGCAAAAA GGGGCAGCTG
    GACAACGACG AACTTGATGA TGGCTATGGG ACGATTTCGC CGGGAACCAT TTTCAAGGAG
    GTGTGGCAGG TGAACGTTAA GCCTAAAGGC TTAGGTCAGA CTCGAAACTT AATGGGGGTG
    TACCGTCTGT GCCTTTCCGC CAAGACTATT CACCTGGTGA AGCTGAACTC GGAGACGCCT
    GCCGTCAACC TGCCGCTAAT GAACATTCGA CGCTGCGGTC ACTCCGAGAG CTTCTTCTTT
    ATCGAGGTGG GCCGCTCTTC TTCCATCGGC CCTGGCGAGA TATGGGTGCA AGTCGAGGAC
    TCTGTTGTGG CGCAGAACAT GCACGAGACC ATTCTAGACA CTATGAAGGC TCTGAAGGCC
    TTTCCTGACT TTAGGCCAAG GAGCAAAAGC CAGTCATCTG GCTCAAATCC GCTGCCGTTT
    ATTACGACTC GCCGGCATCT CGGCAATCTC CCCCCAAGCC AAACTGGCCT CCAGCGAGCC
    TCCCGAACGG ATTCAATGAC AGGCACGCCG CCCACAGGGA AAAGCAGAGG GCGGGGTCAG
    CGCTACCGCA CCTCTAGCGA GGGCGAAGGC ACTCAGGACC GCCCCCTCAG CTCTGGGACG
    GGCAGCTTGG TGCACATCAA TACCCCCATT CTCAGTGTGG GCCGGGAGGA AAGCGGCAGC
    TGCTGCGCTC ATGCATCACC CAATCAGGCC CATCACACCC GCTCCGCATC ACTTCCCGTA
    TCTCACTTCC TGTCTGCCAC CAGCCCGATC AGTGTGTCCA GCAGTAGTGG GCACGGCTCG
    GCTTCAGATA CACACACTCG ACCCTCAAGC TCCTCCATTT GTGGCTCCCC CAGCGACGGA
    GGCTTCAACT CCTCCGACGA GTTCTGCCCC AGCCCCTGCG ACTTCAGGTA CTTTCATGCA
    AGCTGTACCA CACCCGAATC CCACGGCAGC ACCCCGCCGA TTAGGGATGA CTACAGGCTG
    ACTGAGTATA TGACTATGGA TTGGCACAGA GATGGGGCGA GGACGTTCGA GGAGGAGAGC
    AGTTACATGG AGAGGACTTT CCTGAAGCTG GCACACTCGT CCAAGCCAAA GCTAGGCGGC
    AGCTTAAGCA TCACGCAGCA GAAAGCCACG CAGACCTCAT CGTCTTTTGA TGAACCGATG
    GAGTCTAAAC GGCAGCAGGT TTGCTCGTGC CTGCTGAAGT CAGCCTATAA GTCTTACTCT
    GAGTTACATC ACCCAAACAA GCCTCCCTTG CTCAGCTCTG AGTGTCAGAA GAAACTGCCA
    AAAGACGACG GCTACATGCC TATGATCTAC AGCATGGCTT CTTCTCCTAA TGCAGATTAC
    ACCCCTATGC AACCCCGAGC AAACCACCCT GCGTCTCTGG ACGTCCACCC CTGTTCCTCG
    CAGCAGTCGG ACCGGTACGG ATACATGATG ATGCTCCCTA GTGACAGCCC CTCGAGTCGG
    GAGCAGGCGG AGTACGACGA CTACATGGAT ATGTCACAGC TTACAATAGC TGAAGGCTTT
    TCTCAAGCCT CGCCCGATTC CCTCAAGGCC TCGTATTTCT CGCTTCCGCG CTCCTACAAA
    GCCCCGTCAC GAGAAAAGCA CGAAAAGGCC GAATACGTTC CCATGTCTCC AGCTGATCCC
    ATGAGTCCTC CTAGTCCCGG AGAGTATATT CACATGGACT TTGGTCCTCG TCTCGCTCAC
    CCCGCTCTGT CTCCACCCTC CGCAGACTCG CCCTCCACAC CTCCGCCCTC TCAGCCCTGC
    TACCGGATGT GTTGCTCCTC TCCAGACCAT GATTACTTGG GTCTAAACAT GGATAATCTC
    CTAAAGGACC GGCCTCCGCG ACACTCCCTC GTTGCTCCAT GGAATCCCCC TAATTACGCT
    CGCCCCTTAG GGTGCTCATA CGGACAGACC TCCGCTAATT TAGATCCCGA AAAGGTGCAC
    GGTGAATGCG GAGACGTTCC CTTAAGAACC ATGCAGCATC TTTGCATCTC CGAGCGACTG
    CCGTTCACTC ACACGGAGCC CAAAGTCATC AGGGCGGACC CGCAGGGGCG AAGGCGGCAC
    AGTTCGGAGA CTTTCATCCC CTCGCCGGCT ACCTTTGCTA GTGGTTGCGG AATCAGGCCC
    TCAGCCAATC AGAATCTCCC CGAAGCTAGC AGATGGCAGA GCTCCTTCGA TAGCATGTGG
    TCGCATCCGG ACGCCTCCCC GAACTCCGCC ACAGGTGGGA CGCCTCTGAA CACCTCATCC
    AGCTACCTCA ACTACATCGC TCTGGACCTG AGAGAAGTGG CCCGGGTCAC ACACGACGCT
    CCACGCCCAG CCCATAGCAA CCTCCAAGAG AGCGACGCTT ACGCTGGTAT AGACTTCTCC
    GTTTCGGGAG GACGTGCCAA TGCCTCAAAA GATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002666121.3
    CDS630..3785
    Translation

    Target ORF information:

    RefSeq Version XM_002666075.5
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio insulin receptor substrate 4a (irs4a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002666075.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGAAGATC GGCCGCGTTT ACCGAATCAC GGCGTCATGT TGACACTGGA GGCTCAACCG 
    CGTCGCGCTG TCACGAAAAC ATCCGCGAGC GGCATCTCTT GTGCTGCTGC CGGGGAGCCT
    CCATCCTCCT CCTCCTCCTC CTCCTCCTCC TCCACCTCCA CCTCAATCAA TAACAGCGGC
    TGCCGTTTGC CCATGCACTT ATCAGCCTCC TCTCATTGCC AGCCACAGAG GAGGTACCAT
    CCGCCTTACC ACTTTAAAGT GCACCATCTG TTCCCGGATG AGAGCCCTCA GGATGCTGTG
    CTGAGGAAGA ACCTGCCAGC CCTACAGTAT AAGGTGCACG ACTCTGCCTT CTGCCGCGTG
    CTCAGCGCTG ACACCACTGC TGCCGCCCTG GCCGCTGCCG CCGCCTGCTC TGGTGGCCTG
    GACCGGGACA TCTGGAAGTG TGGCTATCTG CGAAAGCAGA AGCACGGCCA CAAGAGGTTC
    TTCGTATTGC GGGGCCCCAG CAACCTCGGG CCCAGCCGCT TGGAGTATTA CGACAGCGAG
    AAAAAATTTC GCAATGCCCT CAAAGCTGCT GCTTCTGGTG TCACGTGCCC TGCCAAAAGG
    GTGATTTATC TGTCGCAGTG TTTTACGGTT AACAAGAGGG CTGATGCTAA GAACAAATAC
    CTCATTGCCC TTTATACTAA AGACGAATAC TTTGCTGTTG TAGCCGAGAG CGAACAGGAG
    CAAGAAGAGT GGTACTTGTC TATAACGGAA CTTATGACGG AGGGCAAAAA GGGGCAGCTG
    GACAACGACG AACTTGATGA TGGCTATGGG ACGATTTCGC CGGGAACCAT TTTCAAGGAG
    GTGTGGCAGG TGAACGTTAA GCCTAAAGGC TTAGGTCAGA CTCGAAACTT AATGGGGGTG
    TACCGTCTGT GCCTTTCCGC CAAGACTATT CACCTGGTGA AGCTGAACTC GGAGACGCCT
    GCCGTCAACC TGCCGCTAAT GAACATTCGA CGCTGCGGTC ACTCCGAGAG CTTCTTCTTT
    ATCGAGGTGG GCCGCTCTTC TTCCATCGGC CCTGGCGAGA TATGGGTGCA AGTCGAGGAC
    TCTGTTGTGG CGCAGAACAT GCACGAGACC ATTCTAGACA CTATGAAGGC TCTGAAGGCC
    TTTCCTGACT TTAGGCCAAG GAGCAAAAGC CAGTCATCTG GCTCAAATCC GCTGCCGTTT
    ATTACGACTC GCCGGCATCT CGGCAATCTC CCCCCAAGCC AAACTGGCCT CCAGCGAGCC
    TCCCGAACGG ATTCAATGAC AGGCACGCCG CCCACAGGGA AAAGCAGAGG GCGGGGTCAG
    CGCTACCGCA CCTCTAGCGA GGGCGAAGGC ACTCAGGACC GCCCCCTCAG CTCTGGGACG
    GGCAGCTTGG TGCACATCAA TACCCCCATT CTCAGTGTGG GCCGGGAGGA AAGCGGCAGC
    TGCTGCGCTC ATGCATCACC CAATCAGGCC CATCACACCC GCTCCGCATC ACTTCCCGTA
    TCTCACTTCC TGTCTGCCAC CAGCCCGATC AGTGTGTCCA GCAGTAGTGG GCACGGCTCG
    GCTTCAGATA CACACACTCG ACCCTCAAGC TCCTCCATTT GTGGCTCCCC CAGCGACGGA
    GGCTTCAACT CCTCCGACGA GTTCTGCCCC AGCCCCTGCG ACTTCAGGTA CTTTCATGCA
    AGCTGTACCA CACCCGAATC CCACGGCAGC ACCCCGCCGA TTAGGGATGA CTACAGGCTG
    ACTGAGTATA TGACTATGGA TTGGCACAGA GATGGGGCGA GGACGTTCGA GGAGGAGAGC
    AGTTACATGG AGAGGACTTT CCTGAAGCTG GCACACTCGT CCAAGCCAAA GCTAGGCGGC
    AGCTTAAGCA TCACGCAGCA GAAAGCCACG CAGACCTCAT CGTCTTTTGA TGAACCGATG
    GAGTCTAAAC GGCAGCAGGT TTGCTCGTGC CTGCTGAAGT CAGCCTATAA GTCTTACTCT
    GAGTTACATC ACCCAAACAA GCCTCCCTTG CTCAGCTCTG AGTGTCAGAA GAAACTGCCA
    AAAGACGACG GCTACATGCC TATGATCTAC AGCATGGCTT CTTCTCCTAA TGCAGATTAC
    ACCCCTATGC AACCCCGAGC AAACCACCCT GCGTCTCTGG ACGTCCACCC CTGTTCCTCG
    CAGCAGTCGG ACCGGTACGG ATACATGATG ATGCTCCCTA GTGACAGCCC CTCGAGTCGG
    GAGCAGGCGG AGTACGACGA CTACATGGAT ATGTCACAGC TTACAATAGC TGAAGGCTTT
    TCTCAAGCCT CGCCCGATTC CCTCAAGGCC TCGTATTTCT CGCTTCCGCG CTCCTACAAA
    GCCCCGTCAC GAGAAAAGCA CGAAAAGGCC GAATACGTTC CCATGTCTCC AGCTGATCCC
    ATGAGTCCTC CTAGTCCCGG AGAGTATATT CACATGGACT TTGGTCCTCG TCTCGCTCAC
    CCCGCTCTGT CTCCACCCTC CGCAGACTCG CCCTCCACAC CTCCGCCCTC TCAGCCCTGC
    TACCGGATGT GTTGCTCCTC TCCAGACCAT GATTACTTGG GTCTAAACAT GGATAATCTC
    CTAAAGGACC GGCCTCCGCG ACACTCCCTC GTTGCTCCAT GGAATCCCCC TAATTACGCT
    CGCCCCTTAG GGTGCTCATA CGGACAGACC TCCGCTAATT TAGATCCCGA AAAGGTGCAC
    GGTGAATGCG GAGACGTTCC CTTAAGAACC ATGCAGCATC TTTGCATCTC CGAGCGACTG
    CCGTTCACTC ACACGGAGCC CAAAGTCATC AGGGCGGACC CGCAGGGGCG AAGGCGGCAC
    AGTTCGGAGA CTTTCATCCC CTCGCCGGCT ACCTTTGCTA GTGGTTGCGG AATCAGGCCC
    TCAGCCAATC AGAATCTCCC CGAAGCTAGC AGATGGCAGA GCTCCTTCGA TAGCATGTGG
    TCGCATCCGG ACGCCTCCCC GAACTCCGCC ACAGGTGGGA CGCCTCTGAA CACCTCATCC
    AGCTACCTCA ACTACATCGC TCTGGACCTG AGAGAAGTGG CCCGGGTCAC ACACGACGCT
    CCACGCCCAG CCCATAGCAA CCTCCAAGAG AGCGACGCTT ACGCTGGTAT AGACTTCTCC
    GTTTCGGGAG GACGTGCCAA TGCCTCAAAA GATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa26239
    Clone ID Related Accession (Same CDS sequence) XM_002666075.5 , XM_002666075.6
    Accession Version XM_002666075.6 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3156bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product insulin receptor substrate 2-B
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007132.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_002666075.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGAAGATC GGCCGCGTTT ACCGAATCAC GGCGTCATGT TGACACTGGA GGCTCAACCG 
    CGTCGCGCTG TCACGAAAAC ATCCGCGAGC GGCATCTCTT GTGCTGCTGC CGGGGAGCCT
    CCATCCTCCT CCTCCTCCTC CTCCTCCTCC TCCACCTCCA CCTCAATCAA TAACAGCGGC
    TGCCGTTTGC CCATGCACTT ATCAGCCTCC TCTCATTGCC AGCCACAGAG GAGGTACCAT
    CCGCCTTACC ACTTTAAAGT GCACCATCTG TTCCCGGATG AGAGCCCTCA GGATGCTGTG
    CTGAGGAAGA ACCTGCCAGC CCTACAGTAT AAGGTGCACG ACTCTGCCTT CTGCCGCGTG
    CTCAGCGCTG ACACCACTGC TGCCGCCCTG GCCGCTGCCG CCGCCTGCTC TGGTGGCCTG
    GACCGGGACA TCTGGAAGTG TGGCTATCTG CGAAAGCAGA AGCACGGCCA CAAGAGGTTC
    TTCGTATTGC GGGGCCCCAG CAACCTCGGG CCCAGCCGCT TGGAGTATTA CGACAGCGAG
    AAAAAATTTC GCAATGCCCT CAAAGCTGCT GCTTCTGGTG TCACGTGCCC TGCCAAAAGG
    GTGATTTATC TGTCGCAGTG TTTTACGGTT AACAAGAGGG CTGATGCTAA GAACAAATAC
    CTCATTGCCC TTTATACTAA AGACGAATAC TTTGCTGTTG TAGCCGAGAG CGAACAGGAG
    CAAGAAGAGT GGTACTTGTC TATAACGGAA CTTATGACGG AGGGCAAAAA GGGGCAGCTG
    GACAACGACG AACTTGATGA TGGCTATGGG ACGATTTCGC CGGGAACCAT TTTCAAGGAG
    GTGTGGCAGG TGAACGTTAA GCCTAAAGGC TTAGGTCAGA CTCGAAACTT AATGGGGGTG
    TACCGTCTGT GCCTTTCCGC CAAGACTATT CACCTGGTGA AGCTGAACTC GGAGACGCCT
    GCCGTCAACC TGCCGCTAAT GAACATTCGA CGCTGCGGTC ACTCCGAGAG CTTCTTCTTT
    ATCGAGGTGG GCCGCTCTTC TTCCATCGGC CCTGGCGAGA TATGGGTGCA AGTCGAGGAC
    TCTGTTGTGG CGCAGAACAT GCACGAGACC ATTCTAGACA CTATGAAGGC TCTGAAGGCC
    TTTCCTGACT TTAGGCCAAG GAGCAAAAGC CAGTCATCTG GCTCAAATCC GCTGCCGTTT
    ATTACGACTC GCCGGCATCT CGGCAATCTC CCCCCAAGCC AAACTGGCCT CCAGCGAGCC
    TCCCGAACGG ATTCAATGAC AGGCACGCCG CCCACAGGGA AAAGCAGAGG GCGGGGTCAG
    CGCTACCGCA CCTCTAGCGA GGGCGAAGGC ACTCAGGACC GCCCCCTCAG CTCTGGGACG
    GGCAGCTTGG TGCACATCAA TACCCCCATT CTCAGTGTGG GCCGGGAGGA AAGCGGCAGC
    TGCTGCGCTC ATGCATCACC CAATCAGGCC CATCACACCC GCTCCGCATC ACTTCCCGTA
    TCTCACTTCC TGTCTGCCAC CAGCCCGATC AGTGTGTCCA GCAGTAGTGG GCACGGCTCG
    GCTTCAGATA CACACACTCG ACCCTCAAGC TCCTCCATTT GTGGCTCCCC CAGCGACGGA
    GGCTTCAACT CCTCCGACGA GTTCTGCCCC AGCCCCTGCG ACTTCAGGTA CTTTCATGCA
    AGCTGTACCA CACCCGAATC CCACGGCAGC ACCCCGCCGA TTAGGGATGA CTACAGGCTG
    ACTGAGTATA TGACTATGGA TTGGCACAGA GATGGGGCGA GGACGTTCGA GGAGGAGAGC
    AGTTACATGG AGAGGACTTT CCTGAAGCTG GCACACTCGT CCAAGCCAAA GCTAGGCGGC
    AGCTTAAGCA TCACGCAGCA GAAAGCCACG CAGACCTCAT CGTCTTTTGA TGAACCGATG
    GAGTCTAAAC GGCAGCAGGT TTGCTCGTGC CTGCTGAAGT CAGCCTATAA GTCTTACTCT
    GAGTTACATC ACCCAAACAA GCCTCCCTTG CTCAGCTCTG AGTGTCAGAA GAAACTGCCA
    AAAGACGACG GCTACATGCC TATGATCTAC AGCATGGCTT CTTCTCCTAA TGCAGATTAC
    ACCCCTATGC AACCCCGAGC AAACCACCCT GCGTCTCTGG ACGTCCACCC CTGTTCCTCG
    CAGCAGTCGG ACCGGTACGG ATACATGATG ATGCTCCCTA GTGACAGCCC CTCGAGTCGG
    GAGCAGGCGG AGTACGACGA CTACATGGAT ATGTCACAGC TTACAATAGC TGAAGGCTTT
    TCTCAAGCCT CGCCCGATTC CCTCAAGGCC TCGTATTTCT CGCTTCCGCG CTCCTACAAA
    GCCCCGTCAC GAGAAAAGCA CGAAAAGGCC GAATACGTTC CCATGTCTCC AGCTGATCCC
    ATGAGTCCTC CTAGTCCCGG AGAGTATATT CACATGGACT TTGGTCCTCG TCTCGCTCAC
    CCCGCTCTGT CTCCACCCTC CGCAGACTCG CCCTCCACAC CTCCGCCCTC TCAGCCCTGC
    TACCGGATGT GTTGCTCCTC TCCAGACCAT GATTACTTGG GTCTAAACAT GGATAATCTC
    CTAAAGGACC GGCCTCCGCG ACACTCCCTC GTTGCTCCAT GGAATCCCCC TAATTACGCT
    CGCCCCTTAG GGTGCTCATA CGGACAGACC TCCGCTAATT TAGATCCCGA AAAGGTGCAC
    GGTGAATGCG GAGACGTTCC CTTAAGAACC ATGCAGCATC TTTGCATCTC CGAGCGACTG
    CCGTTCACTC ACACGGAGCC CAAAGTCATC AGGGCGGACC CGCAGGGGCG AAGGCGGCAC
    AGTTCGGAGA CTTTCATCCC CTCGCCGGCT ACCTTTGCTA GTGGTTGCGG AATCAGGCCC
    TCAGCCAATC AGAATCTCCC CGAAGCTAGC AGATGGCAGA GCTCCTTCGA TAGCATGTGG
    TCGCATCCGG ACGCCTCCCC GAACTCCGCC ACAGGTGGGA CGCCTCTGAA CACCTCATCC
    AGCTACCTCA ACTACATCGC TCTGGACCTG AGAGAAGTGG CCCGGGTCAC ACACGACGCT
    CCACGCCCAG CCCATAGCAA CCTCCAAGAG AGCGACGCTT ACGCTGGTAT AGACTTCTCC
    GTTTCGGGAG GACGTGCCAA TGCCTCAAAA GATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_002666121.3
    CDS431..3586
    Translation

    Target ORF information:

    RefSeq Version XM_002666075.6
    Organism Danio rerio(zebrafish)
    Definition Danio rerio insulin receptor substrate 4a (irs4a), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_002666075.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGGAAGATC GGCCGCGTTT ACCGAATCAC GGCGTCATGT TGACACTGGA GGCTCAACCG 
    CGTCGCGCTG TCACGAAAAC ATCCGCGAGC GGCATCTCTT GTGCTGCTGC CGGGGAGCCT
    CCATCCTCCT CCTCCTCCTC CTCCTCCTCC TCCACCTCCA CCTCAATCAA TAACAGCGGC
    TGCCGTTTGC CCATGCACTT ATCAGCCTCC TCTCATTGCC AGCCACAGAG GAGGTACCAT
    CCGCCTTACC ACTTTAAAGT GCACCATCTG TTCCCGGATG AGAGCCCTCA GGATGCTGTG
    CTGAGGAAGA ACCTGCCAGC CCTACAGTAT AAGGTGCACG ACTCTGCCTT CTGCCGCGTG
    CTCAGCGCTG ACACCACTGC TGCCGCCCTG GCCGCTGCCG CCGCCTGCTC TGGTGGCCTG
    GACCGGGACA TCTGGAAGTG TGGCTATCTG CGAAAGCAGA AGCACGGCCA CAAGAGGTTC
    TTCGTATTGC GGGGCCCCAG CAACCTCGGG CCCAGCCGCT TGGAGTATTA CGACAGCGAG
    AAAAAATTTC GCAATGCCCT CAAAGCTGCT GCTTCTGGTG TCACGTGCCC TGCCAAAAGG
    GTGATTTATC TGTCGCAGTG TTTTACGGTT AACAAGAGGG CTGATGCTAA GAACAAATAC
    CTCATTGCCC TTTATACTAA AGACGAATAC TTTGCTGTTG TAGCCGAGAG CGAACAGGAG
    CAAGAAGAGT GGTACTTGTC TATAACGGAA CTTATGACGG AGGGCAAAAA GGGGCAGCTG
    GACAACGACG AACTTGATGA TGGCTATGGG ACGATTTCGC CGGGAACCAT TTTCAAGGAG
    GTGTGGCAGG TGAACGTTAA GCCTAAAGGC TTAGGTCAGA CTCGAAACTT AATGGGGGTG
    TACCGTCTGT GCCTTTCCGC CAAGACTATT CACCTGGTGA AGCTGAACTC GGAGACGCCT
    GCCGTCAACC TGCCGCTAAT GAACATTCGA CGCTGCGGTC ACTCCGAGAG CTTCTTCTTT
    ATCGAGGTGG GCCGCTCTTC TTCCATCGGC CCTGGCGAGA TATGGGTGCA AGTCGAGGAC
    TCTGTTGTGG CGCAGAACAT GCACGAGACC ATTCTAGACA CTATGAAGGC TCTGAAGGCC
    TTTCCTGACT TTAGGCCAAG GAGCAAAAGC CAGTCATCTG GCTCAAATCC GCTGCCGTTT
    ATTACGACTC GCCGGCATCT CGGCAATCTC CCCCCAAGCC AAACTGGCCT CCAGCGAGCC
    TCCCGAACGG ATTCAATGAC AGGCACGCCG CCCACAGGGA AAAGCAGAGG GCGGGGTCAG
    CGCTACCGCA CCTCTAGCGA GGGCGAAGGC ACTCAGGACC GCCCCCTCAG CTCTGGGACG
    GGCAGCTTGG TGCACATCAA TACCCCCATT CTCAGTGTGG GCCGGGAGGA AAGCGGCAGC
    TGCTGCGCTC ATGCATCACC CAATCAGGCC CATCACACCC GCTCCGCATC ACTTCCCGTA
    TCTCACTTCC TGTCTGCCAC CAGCCCGATC AGTGTGTCCA GCAGTAGTGG GCACGGCTCG
    GCTTCAGATA CACACACTCG ACCCTCAAGC TCCTCCATTT GTGGCTCCCC CAGCGACGGA
    GGCTTCAACT CCTCCGACGA GTTCTGCCCC AGCCCCTGCG ACTTCAGGTA CTTTCATGCA
    AGCTGTACCA CACCCGAATC CCACGGCAGC ACCCCGCCGA TTAGGGATGA CTACAGGCTG
    ACTGAGTATA TGACTATGGA TTGGCACAGA GATGGGGCGA GGACGTTCGA GGAGGAGAGC
    AGTTACATGG AGAGGACTTT CCTGAAGCTG GCACACTCGT CCAAGCCAAA GCTAGGCGGC
    AGCTTAAGCA TCACGCAGCA GAAAGCCACG CAGACCTCAT CGTCTTTTGA TGAACCGATG
    GAGTCTAAAC GGCAGCAGGT TTGCTCGTGC CTGCTGAAGT CAGCCTATAA GTCTTACTCT
    GAGTTACATC ACCCAAACAA GCCTCCCTTG CTCAGCTCTG AGTGTCAGAA GAAACTGCCA
    AAAGACGACG GCTACATGCC TATGATCTAC AGCATGGCTT CTTCTCCTAA TGCAGATTAC
    ACCCCTATGC AACCCCGAGC AAACCACCCT GCGTCTCTGG ACGTCCACCC CTGTTCCTCG
    CAGCAGTCGG ACCGGTACGG ATACATGATG ATGCTCCCTA GTGACAGCCC CTCGAGTCGG
    GAGCAGGCGG AGTACGACGA CTACATGGAT ATGTCACAGC TTACAATAGC TGAAGGCTTT
    TCTCAAGCCT CGCCCGATTC CCTCAAGGCC TCGTATTTCT CGCTTCCGCG CTCCTACAAA
    GCCCCGTCAC GAGAAAAGCA CGAAAAGGCC GAATACGTTC CCATGTCTCC AGCTGATCCC
    ATGAGTCCTC CTAGTCCCGG AGAGTATATT CACATGGACT TTGGTCCTCG TCTCGCTCAC
    CCCGCTCTGT CTCCACCCTC CGCAGACTCG CCCTCCACAC CTCCGCCCTC TCAGCCCTGC
    TACCGGATGT GTTGCTCCTC TCCAGACCAT GATTACTTGG GTCTAAACAT GGATAATCTC
    CTAAAGGACC GGCCTCCGCG ACACTCCCTC GTTGCTCCAT GGAATCCCCC TAATTACGCT
    CGCCCCTTAG GGTGCTCATA CGGACAGACC TCCGCTAATT TAGATCCCGA AAAGGTGCAC
    GGTGAATGCG GAGACGTTCC CTTAAGAACC ATGCAGCATC TTTGCATCTC CGAGCGACTG
    CCGTTCACTC ACACGGAGCC CAAAGTCATC AGGGCGGACC CGCAGGGGCG AAGGCGGCAC
    AGTTCGGAGA CTTTCATCCC CTCGCCGGCT ACCTTTGCTA GTGGTTGCGG AATCAGGCCC
    TCAGCCAATC AGAATCTCCC CGAAGCTAGC AGATGGCAGA GCTCCTTCGA TAGCATGTGG
    TCGCATCCGG ACGCCTCCCC GAACTCCGCC ACAGGTGGGA CGCCTCTGAA CACCTCATCC
    AGCTACCTCA ACTACATCGC TCTGGACCTG AGAGAAGTGG CCCGGGTCAC ACACGACGCT
    CCACGCCCAG CCCATAGCAA CCTCCAAGAG AGCGACGCTT ACGCTGGTAT AGACTTCTCC
    GTTTCGGGAG GACGTGCCAA TGCCTCAAAA GATTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.