wnk1a cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	wnk1a
Entrez Gene ID	100318736
Full Name	WNK lysine deficient protein kinase 1a
Synonyms	im:7165255
General protein information	Preferred Names WNK lysine deficient protein kinase 1a Names serine/threonine-protein kinase WNK1
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_002666846.6	XP_002666892.4	serine/threonine-protein kinase WNK1 isoform X2
XM_009298027.2	XP_009296302.1	serine/threonine-protein kinase WNK1 isoform X1
XM_002666846.5	XP_002666892.3	serine/threonine-protein kinase WNK1 isoform X2
XM_009298027.3	XP_009296302.2	serine/threonine-protein kinase WNK1 isoform X1
XM_021474570.1	XP_021330245.1	serine/threonine-protein kinase WNK1 isoform X3
XM_021470686.1	XP_021326361.1	serine/threonine-protein kinase WNK1 isoform X1
XM_021470687.1	XP_021326362.1	serine/threonine-protein kinase WNK1 isoform X2
XM_021470688.1	XP_021326363.1	serine/threonine-protein kinase WNK1 isoform X3

Pathways from BioSystems
Gene Ontology

The following wnk1a gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the wnk1a cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa141466	XM_021470688.1 Latest version!	Danio rerio WNK lysine deficient protein kinase 1a (wnk1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa146841	XM_021474570.1 Latest version!	Danio rerio WNK lysine deficient protein kinase 1a (wnk1a), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
ODa146839	XM_009298027.3 Latest version!	Danio rerio WNK lysine deficient protein kinase 1a (wnk1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa146840	XM_002666846.6 Latest version!	Danio rerio WNK lysine deficient protein kinase 1a (wnk1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
ODa29433	XM_002666846.5 Latest version!	Danio rerio WNK lysine deficient protein kinase 1a (wnk1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa29433	XM_021470687.1 Latest version!	Danio rerio WNK lysine deficient protein kinase 1a (wnk1a), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa49115	XM_009298027.2 Latest version!	Danio rerio WNK lysine deficient protein kinase 1a (wnk1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00
ODa49115	XM_021470686.1 Latest version!	Danio rerio WNK lysine deficient protein kinase 1a (wnk1a), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	25	$881.30 $1259.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

wnk1a ( XM_021470688.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326363.1 Danio rerio WNK lysine deficient protein kinase 1a (wnk1a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa141466
Clone ID Related Accession (Same CDS sequence)	XM_021470688.1
Accession Version	XM_021470688.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3795bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCTG AGATGTATGA AGAGAAGTAT GACGAGTCGG TGGATGTGTA TGCCTTTGGG 
ATGTGTATGC TGGAGATGGC TACTTCTGAG TACCCGTACT CCGAGTGTCA GAATGCAGCT 
CAGATCTATC GCAGAGTCAC CAGCGGGGTG AAGCCTGGCA GTTTTGACAA AGTGGCAATT 
CCAGAGGTGA AGGAGATCAT TGAGGGTTGC ATTCGTCAGA ATAAAGATGA GAGGTACTGC 
ATCAAGGATC TCCTAAGCCA TGCCTTCTTC CAGGAGGAGA CTGGAGTTCG TGTAGAACTG 
GCTGAGGAGG ATGATGGAGA GCTTGTGGCT ATAAAATTGT TGCTGCGCAT TGAGGATGTT 
AAGAAACTTA AAGGCAAATA CAAAGAGAAT GAAGCCATCG AGTTTTCCTT CGACTTGGAT 
AAAGACGTCC CAGATGATGT GGCTCAAGAA ATGGTTGAAT CAGGCTACAT TTGTGAGGGT 
GACCACAAAA CCATTGCAAA GGCCATAAAG GACAGAGTGT CCCTGATCTC CCGTAAGAGA 
GAGCAGAGAA AACTGGTGAG AGAAGAACAG GAAAAAAGGA AGATGGAGCA GGAGAATGAC 
GCCGCCCAGC AAACTAACCC CACAGTCAAG TCCCCTGGTT CTGCTCTGGC CTCAGTCATA 
ATGGAGTCTG AAGAAGCAGA AACAGACCAG CACCAGCAGC CTGGAGCCTC TGCTTCTGCT 
GTAGGGTTTG TGGATCCTCA AAGTTCAGTG ACCATCCTCG AGGCTCATCC TGTCCAGCCA 
AACACATCTT TCAGCACCAC ACAGCCTGAT CCGCAGCCAC AGCACACAAT TATACGTCCA 
CAAAGCATGT CGCAGCACCC AGTCCAGGCC CCTCTGCAGC AGCCGAGCAG TACTTTGACC 
CTGGGCCCCG TCCCCCAGAC TTCTCCACCT CAGGTGATCG GTCAAACAGC CTCTCTGCCC 
TCTACTGTTC AATCTATTGT CCCGCTGCAG TACCGTCAGC CACCTCAGGA TGGAATCACA 
CTACCTCAAT CCCTGCCCAG CGCTCAAGTC TTACAGATAT GGGCATGTCA ACTCAGCCCT 
CCTGTTTTCT CACCACCTCC AAATGCCGAA CAGCTCTATT TTCTATATCA GGCCTGTCAG 
GCACCACTAA GTGTGTCCCA ATCACAACCA GTAGAGCAGA CACAACCTCA AACATCTCTG 
GTTCCTGCCT CCACAGAAAG TGGACACTCT GATCCGGCAT CTGGTCTAAG CGATGGGAAT 
GAGGGGAGAC ACGAGGGTCG CTCTATGAAA CGACCCCAAC GCCGTTCTGT ACGCAGCCGC 
TCGCGTCATG AAAAAACAGC TAAGGTCAAG CTCAATGTGC TAAATATTTC TAATATTGGA 
GACAGAGTCG CTGAATGTCA GCTGGAAACC CACAACAGAA AGATGGTCAC ATTCAAGTTT 
GATCTGGATG GGGATAATCC TGTAGAAATC GCTGAAATTA TGGTCAAAAG TGATTTTATT 
TTGGAGAGTG AACGAGAGTC CTTCATAGAA CAGGTTCAGC TGGTCATTCA GAATGCAGAT 
CAGAAAGGAA AAGCTCCCCA AAATAGCCAA ACTCAGGTGA TCAGTAATTC GAGCCAACAA 
GTACCTGAAA TTTTAACATC CAGTGTGCCT GGTATGCCAC CTAGCTTAGC AGCACAGGTT 
GTACATTCAG CAGGCAGGCG CTTCATAGTC AGCCCCGTGC CTGAAGCACG ACTTCGGGAG 
CCGTTGTTTG GTACTCCCTC AGCAAACACA TCATTTGAAG ATACGGCACC AGCACCTGAT 
CCAACATCAG CCAGCAGTGC TCCGCAGGAC CAAGCCAACT TTGCCTCTGA TTCAAACCAA 
ATTTCTGTGG TACCACCGAC ACCTGGAGAT CTTGCCCCAG AGAGCTCCAT TCCACCTACT 
TCTCAATCAC CATACCAGAA TCCTGTCCCA TCATCCACTG CAACTTTGAC TGTGACCACC 
ACTCAAGTAA GCACTCCAAC ACCAACTACC ACTCCGCAAC CTATAACAGG GAGGACGTCT 
CCTTCACCAG CCCCAACAGA ACCACCAGCA GCACGTCGTA TGTCACTTCC CAGCCCATCC 
CAGACACCTC AAATTGCTCC AGGAGAGAGT AATGGCTTGG ATCAAGCACA AGCCTCAAAT 
CAGCAAGTAA CTCAACCGAG TGCAACCGGA GATAGTGAGA GCGACGTACA GGGGAAACCA 
CCTGGAATTG AAGACATTCA TGCTCTAGAC CAAAAGCTGC GCTCCCTTTT CAAGGACTCA 
TCCCAGAACT CTTGCAGCCA ACCTGACGGG TCTGGAGAGA CTTCCACAAC CTCTCCACCC 
AACACCATAA GCACTAACAC TCCTGGCTTT GTCTCTGGTG TGACGCCTTC TAGTTTGTCG 
CTCAATTCCA GCGGGAACTT TGCACCTGGT GCTTTCGCCT CCATTGGTAC TCCTACAAGC 
TACACCCAGA CACCATCTAC CGACCAGTCC CCTCCAAGGC CACAGATTGC TGTTGGTGTA 
ACTCAACCAG ATGGACACAA CAGTACTGAG GAGAAAGAGC CACAACTTGA GAAAGCTTTC 
CAACTTGGCC GATTTCAGGT GTCTGTGGCC AGTGATCAAG ACCCACCAAC TGCTCTAGAC 
CCAGGAGTGG TGGTTTCATC TTCCTCTGCT ACCCCATCTT CAACATCATC ATCATCATCA 
TCTTCTTCAT CACCATCATC TCTCTCTAGC CCCGAAAACA CAATTCATAA ATCTCAGACT 
CCTCCACCCA GAGTCAGCCC TGAACCCGTA CCACCTACCA CTACAGTTGG CCGCTTTCAG 
GTGACTTGCA GCACTGACAT CAAACTTGGT CGCTTCTCAG TGACACCAGC AGAAAGAAAG 
TCTTCCTCAG TTGAGGGACC AGACACCGCC AAAGAGCCTA AATTGACCCC TTCCAGTGTG 
TCCATTACCA ATCATTATCT CAGTAGCGAC AACGACTCAG AACCAGAAGA TGAGGGCTTA 
AGACGAGAGA TGAACAGGCT CAAGGAGAGA CATAAGGTTG AGATCATGGC ATTGCAGAAT 
AAACAGAAAA AGGAAATTGA GGAGCTCTTC ACTCGTCTGG GCAAAACACC ATCTGTGGTT 
GTGCCACCCA CTGTGGCCAT GACGCCTGGT AGACGGAGAC CTGCTAAGGG CAAGGGGAAC 
AGGCCAAGAA GTGGTAGTGC CACTCAGGCT GCATCTTTTC AACAAGGGAA CAAACAAGCT 
GTGCAGAGTG GTTCACTCAG TCAGTCAGCC AGTGAGAAGA CTTCACCTTC AGAAACAGCA 
CCAAGCTCAA ACAATGATGT GCAAGATAAT ACAAGTCCAG TGAGAAAAGA AAACGGACAG 
AGCCATGACC AGGACCAAAC ACCAACAACT AATCTGCCAC GCAAGGGCAC ATTCACAGAT 
GACCTCCATC AGCTGGTTGA TAACTGGGCC AGAGATGCCA TGAACATGAT GCCACAGGTC 
AAGAAAGGAT CCAAAAAGAA TGTTCATGAA ATGCCACGTA AATACTCCGC ACCAGGTCAG 
CTTTGTTCCA CTGGTGGCCA CATCCCAGCG ACCATCAACC CCACAGCTGG ACGGAAGGGT 
TCAATAGGTG TGGCCAATCA GCAATTTGGC TACACTTGTC CCAACTACAA CATGCCTCAA 
TGGAATCAGG TGGGGACAAT ATCATCCACC GCTCCACCTG CAGGACTACA GCAGGGTTTC 
CTTATCCCAT CAGGAGCCCA CCAAACCGGA AATAACTGCG GATCAAGCAA TCTGCGCACC 
ACTCAGGCAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326363.1
CDS	255..4049
Translation	MAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYCIKDLLSHAFFQEETGVRVELAEEDDGELVAIKLLLRIEDVKKLKGKYKENEAIEFSFDLDKDVPDDVAQEMVESGYICEGDHKTIAKAIKDRVSLISRKREQRKLVREEQEKRKMEQENDAAQQTNPTVKSPGSALASVIMESEEAETDQHQQPGASASAVGFVDPQSSVTILEAHPVQPNTSFSTTQPDPQPQHTIIRPQSMSQHPVQAPLQQPSSTLTLGPVPQTSPPQVIGQTASLPSTVQSIVPLQYRQPPQDGITLPQSLPSAQVLQIWACQLSPPVFSPPPNAEQLYFLYQACQAPLSVSQSQPVEQTQPQTSLVPASTESGHSDPASGLSDGNEGRHEGRSMKRPQRRSVRSRSRHEKTAKVKLNVLNISNIGDRVAECQLETHNRKMVTFKFDLDGDNPVEIAEIMVKSDFILESERESFIEQVQLVIQNADQKGKAPQNSQTQVISNSSQQVPEILTSSVPGMPPSLAAQVVHSAGRRFIVSPVPEARLREPLFGTPSANTSFEDTAPAPDPTSASSAPQDQANFASDSNQISVVPPTPGDLAPESSIPPTSQSPYQNPVPSSTATLTVTTTQVSTPTPTTTPQPITGRTSPSPAPTEPPAARRMSLPSPSQTPQIAPGESNGLDQAQASNQQVTQPSATGDSESDVQGKPPGIEDIHALDQKLRSLFKDSSQNSCSQPDGSGETSTTSPPNTISTNTPGFVSGVTPSSLSLNSSGNFAPGAFASIGTPTSYTQTPSTDQSPPRPQIAVGVTQPDGHNSTEEKEPQLEKAFQLGRFQVSVASDQDPPTALDPGVVVSSSSATPSSTSSSSSSSSSPSSLSSPENTIHKSQTPPPRVSPEPVPPTTTVGRFQVTCSTDIKLGRFSVTPAERKSSSVEGPDTAKEPKLTPSSVSITNHYLSSDNDSEPEDEGLRREMNRLKERHKVEIMALQNKQKKEIEELFTRLGKTPSVVVPPTVAMTPGRRRPAKGKGNRPRSGSATQAASFQQGNKQAVQSGSLSQSASEKTSPSETAPSSNNDVQDNTSPVRKENGQSHDQDQTPTTNLPRKGTFTDDLHQLVDNWARDAMNMMPQVKKGSKKNVHEMPRKYSAPGQLCSTGGHIPATINPTAGRKGSIGVANQQFGYTCPNYNMPQWNQVGTISSTAPPAGLQQGFLIPSGAHQTGNNCGSSNLRTTQAS

Target ORF information:

RefSeq Version	XM_021470688.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1a (wnk1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470688.1

ORF Insert Sequence:

ATGGCGCCTG AGATGTATGA AGAGAAGTAT GACGAGTCGG TGGATGTGTA TGCCTTTGGG 
ATGTGTATGC TGGAGATGGC TACTTCTGAG TACCCGTACT CCGAGTGTCA GAATGCAGCT 
CAGATCTATC GCAGAGTCAC CAGCGGGGTG AAGCCTGGCA GTTTTGACAA AGTGGCAATT 
CCAGAGGTGA AGGAGATCAT TGAGGGTTGC ATTCGTCAGA ATAAAGATGA GAGGTACTGC 
ATCAAGGATC TCCTAAGCCA TGCCTTCTTC CAGGAGGAGA CTGGAGTTCG TGTAGAACTG 
GCTGAGGAGG ATGATGGAGA GCTTGTGGCT ATAAAATTGT TGCTGCGCAT TGAGGATGTT 
AAGAAACTTA AAGGCAAATA CAAAGAGAAT GAAGCCATCG AGTTTTCCTT CGACTTGGAT 
AAAGACGTCC CAGATGATGT GGCTCAAGAA ATGGTTGAAT CAGGCTACAT TTGTGAGGGT 
GACCACAAAA CCATTGCAAA GGCCATAAAG GACAGAGTGT CCCTGATCTC CCGTAAGAGA 
GAGCAGAGAA AACTGGTGAG AGAAGAACAG GAAAAAAGGA AGATGGAGCA GGAGAATGAC 
GCCGCCCAGC AAACTAACCC CACAGTCAAG TCCCCTGGTT CTGCTCTGGC CTCAGTCATA 
ATGGAGTCTG AAGAAGCAGA AACAGACCAG CACCAGCAGC CTGGAGCCTC TGCTTCTGCT 
GTAGGGTTTG TGGATCCTCA AAGTTCAGTG ACCATCCTCG AGGCTCATCC TGTCCAGCCA 
AACACATCTT TCAGCACCAC ACAGCCTGAT CCGCAGCCAC AGCACACAAT TATACGTCCA 
CAAAGCATGT CGCAGCACCC AGTCCAGGCC CCTCTGCAGC AGCCGAGCAG TACTTTGACC 
CTGGGCCCCG TCCCCCAGAC TTCTCCACCT CAGGTGATCG GTCAAACAGC CTCTCTGCCC 
TCTACTGTTC AATCTATTGT CCCGCTGCAG TACCGTCAGC CACCTCAGGA TGGAATCACA 
CTACCTCAAT CCCTGCCCAG CGCTCAAGTC TTACAGATAT GGGCATGTCA ACTCAGCCCT 
CCTGTTTTCT CACCACCTCC AAATGCCGAA CAGCTCTATT TTCTATATCA GGCCTGTCAG 
GCACCACTAA GTGTGTCCCA ATCACAACCA GTAGAGCAGA CACAACCTCA AACATCTCTG 
GTTCCTGCCT CCACAGAAAG TGGACACTCT GATCCGGCAT CTGGTCTAAG CGATGGGAAT 
GAGGGGAGAC ACGAGGGTCG CTCTATGAAA CGACCCCAAC GCCGTTCTGT ACGCAGCCGC 
TCGCGTCATG AAAAAACAGC TAAGGTCAAG CTCAATGTGC TAAATATTTC TAATATTGGA 
GACAGAGTCG CTGAATGTCA GCTGGAAACC CACAACAGAA AGATGGTCAC ATTCAAGTTT 
GATCTGGATG GGGATAATCC TGTAGAAATC GCTGAAATTA TGGTCAAAAG TGATTTTATT 
TTGGAGAGTG AACGAGAGTC CTTCATAGAA CAGGTTCAGC TGGTCATTCA GAATGCAGAT 
CAGAAAGGAA AAGCTCCCCA AAATAGCCAA ACTCAGGTGA TCAGTAATTC GAGCCAACAA 
GTACCTGAAA TTTTAACATC CAGTGTGCCT GGTATGCCAC CTAGCTTAGC AGCACAGGTT 
GTACATTCAG CAGGCAGGCG CTTCATAGTC AGCCCCGTGC CTGAAGCACG ACTTCGGGAG 
CCGTTGTTTG GTACTCCCTC AGCAAACACA TCATTTGAAG ATACGGCACC AGCACCTGAT 
CCAACATCAG CCAGCAGTGC TCCGCAGGAC CAAGCCAACT TTGCCTCTGA TTCAAACCAA 
ATTTCTGTGG TACCACCGAC ACCTGGAGAT CTTGCCCCAG AGAGCTCCAT TCCACCTACT 
TCTCAATCAC CATACCAGAA TCCTGTCCCA TCATCCACTG CAACTTTGAC TGTGACCACC 
ACTCAAGTAA GCACTCCAAC ACCAACTACC ACTCCGCAAC CTATAACAGG GAGGACGTCT 
CCTTCACCAG CCCCAACAGA ACCACCAGCA GCACGTCGTA TGTCACTTCC CAGCCCATCC 
CAGACACCTC AAATTGCTCC AGGAGAGAGT AATGGCTTGG ATCAAGCACA AGCCTCAAAT 
CAGCAAGTAA CTCAACCGAG TGCAACCGGA GATAGTGAGA GCGACGTACA GGGGAAACCA 
CCTGGAATTG AAGACATTCA TGCTCTAGAC CAAAAGCTGC GCTCCCTTTT CAAGGACTCA 
TCCCAGAACT CTTGCAGCCA ACCTGACGGG TCTGGAGAGA CTTCCACAAC CTCTCCACCC 
AACACCATAA GCACTAACAC TCCTGGCTTT GTCTCTGGTG TGACGCCTTC TAGTTTGTCG 
CTCAATTCCA GCGGGAACTT TGCACCTGGT GCTTTCGCCT CCATTGGTAC TCCTACAAGC 
TACACCCAGA CACCATCTAC CGACCAGTCC CCTCCAAGGC CACAGATTGC TGTTGGTGTA 
ACTCAACCAG ATGGACACAA CAGTACTGAG GAGAAAGAGC CACAACTTGA GAAAGCTTTC 
CAACTTGGCC GATTTCAGGT GTCTGTGGCC AGTGATCAAG ACCCACCAAC TGCTCTAGAC 
CCAGGAGTGG TGGTTTCATC TTCCTCTGCT ACCCCATCTT CAACATCATC ATCATCATCA 
TCTTCTTCAT CACCATCATC TCTCTCTAGC CCCGAAAACA CAATTCATAA ATCTCAGACT 
CCTCCACCCA GAGTCAGCCC TGAACCCGTA CCACCTACCA CTACAGTTGG CCGCTTTCAG 
GTGACTTGCA GCACTGACAT CAAACTTGGT CGCTTCTCAG TGACACCAGC AGAAAGAAAG 
TCTTCCTCAG TTGAGGGACC AGACACCGCC AAAGAGCCTA AATTGACCCC TTCCAGTGTG 
TCCATTACCA ATCATTATCT CAGTAGCGAC AACGACTCAG AACCAGAAGA TGAGGGCTTA 
AGACGAGAGA TGAACAGGCT CAAGGAGAGA CATAAGGTTG AGATCATGGC ATTGCAGAAT 
AAACAGAAAA AGGAAATTGA GGAGCTCTTC ACTCGTCTGG GCAAAACACC ATCTGTGGTT 
GTGCCACCCA CTGTGGCCAT GACGCCTGGT AGACGGAGAC CTGCTAAGGG CAAGGGGAAC 
AGGCCAAGAA GTGGTAGTGC CACTCAGGCT GCATCTTTTC AACAAGGGAA CAAACAAGCT 
GTGCAGAGTG GTTCACTCAG TCAGTCAGCC AGTGAGAAGA CTTCACCTTC AGAAACAGCA 
CCAAGCTCAA ACAATGATGT GCAAGATAAT ACAAGTCCAG TGAGAAAAGA AAACGGACAG 
AGCCATGACC AGGACCAAAC ACCAACAACT AATCTGCCAC GCAAGGGCAC ATTCACAGAT 
GACCTCCATC AGCTGGTTGA TAACTGGGCC AGAGATGCCA TGAACATGAT GCCACAGGTC 
AAGAAAGGAT CCAAAAAGAA TGTTCATGAA ATGCCACGTA AATACTCCGC ACCAGGTCAG 
CTTTGTTCCA CTGGTGGCCA CATCCCAGCG ACCATCAACC CCACAGCTGG ACGGAAGGGT 
TCAATAGGTG TGGCCAATCA GCAATTTGGC TACACTTGTC CCAACTACAA CATGCCTCAA 
TGGAATCAGG TGGGGACAAT ATCATCCACC GCTCCACCTG CAGGACTACA GCAGGGTTTC 
CTTATCCCAT CAGGAGCCCA CCAAACCGGA AATAACTGCG GATCAAGCAA TCTGCGCACC 
ACTCAGGCAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1a ( XM_021474570.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021330245.1 Danio rerio WNK lysine deficient protein kinase 1a (wnk1a), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa146841
Clone ID Related Accession (Same CDS sequence)	XM_021474570.1
Accession Version	XM_021474570.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3792bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395370.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGCCTG AGATGTATGA AGAGAAGTAT GACGAGTCGG TGGATGTGTA TGCCTTTGGG 
ATGTGTATGC TGGAGATGGC TACTTCTGAG TACCCGTACT CCGAGTGTCA GAATGCAGCT 
CAGATCTATC GCAGAGTCAC CAGCGGGGTG AAGCCTGGCA GTTTTGACAA AGTGGCTATT 
CCAGAGGTGA AGGAGATCAT TGAGGGTTGC ATTCGTCAGA ATAAAGATGA GAGGTACTGC 
ATCAAGGATC TCCTAAGCCA TGCCTTCTTC CAGGAGGAGA CTGGAGTTCG TGTAGAACTG 
GCTGAGGAGG ATGATGGAGA GCTTGTGGCT ATAAAGTTGT TGCTGCGCAT TGAGGATGTT 
AAGAAACTTA AAGGCAAATA CAAAGAGAAT GAAGCCATCG AGTTTTCCTT CGACTTGGAT 
AAAGACAACC CAGATGATGT GGCTCAAGAA ATGGTTGAAT CAGGCTACAT TTGTGAGGGT 
GACCACAAAA CCATTGCAAA GGCCATAAAG GACAGAGTGT CCCTGATCTC CCGTAAGAGA 
GAGCAGAGAA AACTGGTGAG AGAAGAACAG GAAAAAAGGA AGATGGAGCA GGAGAATGAC 
GCCGCCCAGC AAACTAACCC CACAGTCAAG TCCCCTGGTT CTGCTCTGGC CTCGGTCATA 
ATGGAGTCTG AAGAAGCAGA AACAGACCAG CACCAGCAGC CTGGAGCCTC TGCTTCTGCT 
GTAGGGTTTG TGGATCCTCA AAGTTCAGTG ACCATCCTCG AGGCTCATCC TGTCCAGCCA 
AACACATCTT TCAGCACCAC ACAGCCTGAT CCGCAGCCAC AGCACACAAT TATACGTCCA 
CAAAGCATGT CGCAGCACCC AGTCCAGGCC CCTCTGCAGC AGCCGAGCAG TACTTTGACC 
CTGGGCCCCG TCCCCCAGAC TTCTCCACCT CAGGTGATTG GTCAAACAGC CTCTCTGCCC 
TCTACTGTTC AACCTATTGT CCCGCTGCAG TACCGTCAGC CACCTCAGGA TGGAATCACA 
CTACCTCAAT CCCTGCCCAG CGCTCAAGTC TTACAGATAT GGGCATGTCA ACTCAGCCCT 
CCTGTTTTCT CACCACCTCC AAATGCCGAA CAGCTCTATT TTCTATATCA GGCCTGTCAG 
GCACCACTAA GTGTGTCCCA ATCACAACCA GTAGAGCAGA CACAACCTCA AACATCTCTG 
GTTCCTGCCT CCACAGAAAG TGGACACTCT GATCCGGCAT CTGGTCTAAG CGATGGGAAT 
GAGGGGAGAC ACGAGGGTCG CTCTATGAAA CGACCCCAAC GCCGTTCTGT ACGCAGCCGC 
TCGCGTCATG AAAAAACAGC TAAGGTCAAG CTCAATGTGC TAAATATTTC TAATATTGGA 
GACAGAGTCG CTGAATGTCA GCTGGAAACC CACAACAGAA AGATGGTCAC ATTCAAGTTT 
GATCTGGATG GGGATAATCC TGTAGAAATC GCTGAAATTA TGGTCAAAAG TGATTTTATT 
TTGGAGAGTG AACGAGAGTC CTTCATAGAA CAGGTTCAAC TGGTCATTCA GAATGCAGAT 
CAGAAAGGAA AAGCTCCCCA AAATAGCCAA ACTCAGGTGA TCAGTAATTC GAGCCAACAA 
GTACCTGAAA TTTTAACATC CAGTGTGCCT GGTATGCCAC CTAGCTTAGC AGCACAGGTT 
GTACATTCAG CTGGCAGGCG CTTCATAGTC AGCCCCGTGC CTGAAGCACG ACTTCGGGAG 
CCGTTGTTTG GTACTCCCTC AGCAAACACA TCATTTGAAG ATACAGCACC AGCACCTGAT 
CCAACATCAG CCAGCAGTGC TCCGCAGGAC CAAGCCAACT TTGCCTCTGA TTCAAACCAA 
ATTTCTGTGG TACCACCGAC ACCTGGAGGT CTTGCCCCAG AGAGCTCCAT TCCACCTACT 
TCTCAATCAC CATACCAGAA TCCTGTCCCA TCATCCACTG CAACTTTGAC TGTGACCACC 
ACTCAAGTAA CCACTCCAAC ACCAACTACC ACTCCGCAAC CTATAACAGG GAGGACGTCT 
CCTTCACCAG CCCCAACAGA ACCACCAGCA GCACGTCGTA TGTCACTTCC CAGCCCATCC 
CAGACACCTC AAATTGCTCC AGGAGAGAGT AATGGCTTGG ATCAAGCACA AGCCTCAAAT 
CAGCAAGTAA CTCAACCGAG TGCAACCGGA GATAGTGAGA GCGATGTACA GGGGAAACCA 
CCTGGAATTG AAGACATTCA TGCTCTAGAC CAAAAGCTGC GCTCCCTTTT CAAGGACTCA 
TCCCAGAACT CTTGCAGCCA ACCTGACGGG TCTGGAGAGA CTTCCACAAC CTCTCCACCC 
AACACCATAA GCACTAACAC TCCTGGCTTT GTCTCTGGTG TGACGCCTTC AAGTTTGTCG 
CTCAATTCCA GCGGGAACTT TGCACCTGGT GCTTTCGCCT CCATTGGTAC TCCTACAAGC 
TACACCCAGA CACCATCTAC CGACCAGTCC CCTCCAAGGC CACAGATTTC TGTAGGTGTA 
ACTCAACCAG ATGGACACAA CAGTACTGAG GAGAAAGAAC CACAACTTGA GAAAGCTTTC 
CAACTTGGCC GATTTCAGGT GTCTGTGGCC AGTGATCAAG ACCCACCAAC TGCTCTAGAC 
CCAGGAGTGG TGGTTTCATC TTCCTCTGCT ACCCCATCTT CAACATCATC ATCATCTTCT 
TCTTCATCAC CATCATCTCT CTCTAGCCCC GAAAACACAA TTCATAAATC TCAGACTCCT 
CCACCCAGGG TCAGCCCTGA ACCCGTACCA CCTACCACTA CAGTTGGCCG CTTTCAGGTG 
ACCTCCAGCA CTGACATCAA ACTTGGTCGC TTCTCAGTGA CACCAGCAGA AAGAAAGTCT 
TCCTCAGTTG AGGGACCAGA CACCGCCAAA GAGCCTAAAT TGACCCCTTC CAGTGTGTCC 
ATTACCAATC ACTATCTCAG TAGCGACAAC GACTCAGAAC CAGAAGATGA GGGCTTAAGA 
CGAGAGATGA ACAGGCTCAA GGAGAGACAT AAGGTTGAGA TCATGGCATT GCAGAATAAA 
CAGAAAAAGG AAATCGAGGA GCTCTTCACT CGTCTGGGCA AAACACCATC TGTGGTTGTG 
CCACCCACTG TGGCCATGAC GCCTGGTAGA CGGAGACCTG CTAAGGGCAA GGGGAACAGG 
CCAAGAAGTG GTAGTGCCAC TCAGGCTGCA TCTTTTCAAC AAGGGAACAA ACAAGCTGTG 
CAGAGTGGTT CACTCAGTCA GTCAGCCAGT GAGAAGACTT CACCTTCAGA AACAGCACCA 
AGCTCAAACA ATGATGTGCA AGATAATACA AGTCCAGTGA GAAAAGAAAA CGGACAGAGC 
CATGACCAGG ACCAAACACC GACAACTAAT CTGCCACGCA AGGGCACATT CACAGATGAC 
CTCCATCAGC TGGTTGATAA CTGGGCCAGA GATGCCATGA ACATGATGCC ACAGGTCAAG 
AAAGGATCCA AAAAGAATGT TCATGAAATG CCACGAAAAT ACTCCGCACC AGGTCAGCTT 
TGTTCCACTG GTGGCCACAT CCCAGCGACC ATCAACCCCA CAGCTGGACG GAAGGGTTCA 
ATAGGTGTGG CCAATCAGCA ATTTGGCTAC ACTTGTCCCA ACTACAACAT GCCTCAATGG 
AATCAGGTGG GGACAATATC ATCCACCGCT CCACCTGCAG GACTACAGCA GGGTTTCCTT 
ATCCCATCAG GAGCCCACCA AACCGGAAAT AACTGCGGAT CAAGCAATCT GCGCACCACT 
CAGGCAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021330245.1
CDS	255..4046
Translation	MAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYCIKDLLSHAFFQEETGVRVELAEEDDGELVAIKLLLRIEDVKKLKGKYKENEAIEFSFDLDKDNPDDVAQEMVESGYICEGDHKTIAKAIKDRVSLISRKREQRKLVREEQEKRKMEQENDAAQQTNPTVKSPGSALASVIMESEEAETDQHQQPGASASAVGFVDPQSSVTILEAHPVQPNTSFSTTQPDPQPQHTIIRPQSMSQHPVQAPLQQPSSTLTLGPVPQTSPPQVIGQTASLPSTVQPIVPLQYRQPPQDGITLPQSLPSAQVLQIWACQLSPPVFSPPPNAEQLYFLYQACQAPLSVSQSQPVEQTQPQTSLVPASTESGHSDPASGLSDGNEGRHEGRSMKRPQRRSVRSRSRHEKTAKVKLNVLNISNIGDRVAECQLETHNRKMVTFKFDLDGDNPVEIAEIMVKSDFILESERESFIEQVQLVIQNADQKGKAPQNSQTQVISNSSQQVPEILTSSVPGMPPSLAAQVVHSAGRRFIVSPVPEARLREPLFGTPSANTSFEDTAPAPDPTSASSAPQDQANFASDSNQISVVPPTPGGLAPESSIPPTSQSPYQNPVPSSTATLTVTTTQVTTPTPTTTPQPITGRTSPSPAPTEPPAARRMSLPSPSQTPQIAPGESNGLDQAQASNQQVTQPSATGDSESDVQGKPPGIEDIHALDQKLRSLFKDSSQNSCSQPDGSGETSTTSPPNTISTNTPGFVSGVTPSSLSLNSSGNFAPGAFASIGTPTSYTQTPSTDQSPPRPQISVGVTQPDGHNSTEEKEPQLEKAFQLGRFQVSVASDQDPPTALDPGVVVSSSSATPSSTSSSSSSSSPSSLSSPENTIHKSQTPPPRVSPEPVPPTTTVGRFQVTSSTDIKLGRFSVTPAERKSSSVEGPDTAKEPKLTPSSVSITNHYLSSDNDSEPEDEGLRREMNRLKERHKVEIMALQNKQKKEIEELFTRLGKTPSVVVPPTVAMTPGRRRPAKGKGNRPRSGSATQAASFQQGNKQAVQSGSLSQSASEKTSPSETAPSSNNDVQDNTSPVRKENGQSHDQDQTPTTNLPRKGTFTDDLHQLVDNWARDAMNMMPQVKKGSKKNVHEMPRKYSAPGQLCSTGGHIPATINPTAGRKGSIGVANQQFGYTCPNYNMPQWNQVGTISSTAPPAGLQQGFLIPSGAHQTGNNCGSSNLRTTQAS

Target ORF information:

RefSeq Version	XM_021474570.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1a (wnk1a), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021474570.1

ORF Insert Sequence:

ATGGCGCCTG AGATGTATGA AGAGAAGTAT GACGAGTCGG TGGATGTGTA TGCCTTTGGG 
ATGTGTATGC TGGAGATGGC TACTTCTGAG TACCCGTACT CCGAGTGTCA GAATGCAGCT 
CAGATCTATC GCAGAGTCAC CAGCGGGGTG AAGCCTGGCA GTTTTGACAA AGTGGCTATT 
CCAGAGGTGA AGGAGATCAT TGAGGGTTGC ATTCGTCAGA ATAAAGATGA GAGGTACTGC 
ATCAAGGATC TCCTAAGCCA TGCCTTCTTC CAGGAGGAGA CTGGAGTTCG TGTAGAACTG 
GCTGAGGAGG ATGATGGAGA GCTTGTGGCT ATAAAGTTGT TGCTGCGCAT TGAGGATGTT 
AAGAAACTTA AAGGCAAATA CAAAGAGAAT GAAGCCATCG AGTTTTCCTT CGACTTGGAT 
AAAGACAACC CAGATGATGT GGCTCAAGAA ATGGTTGAAT CAGGCTACAT TTGTGAGGGT 
GACCACAAAA CCATTGCAAA GGCCATAAAG GACAGAGTGT CCCTGATCTC CCGTAAGAGA 
GAGCAGAGAA AACTGGTGAG AGAAGAACAG GAAAAAAGGA AGATGGAGCA GGAGAATGAC 
GCCGCCCAGC AAACTAACCC CACAGTCAAG TCCCCTGGTT CTGCTCTGGC CTCGGTCATA 
ATGGAGTCTG AAGAAGCAGA AACAGACCAG CACCAGCAGC CTGGAGCCTC TGCTTCTGCT 
GTAGGGTTTG TGGATCCTCA AAGTTCAGTG ACCATCCTCG AGGCTCATCC TGTCCAGCCA 
AACACATCTT TCAGCACCAC ACAGCCTGAT CCGCAGCCAC AGCACACAAT TATACGTCCA 
CAAAGCATGT CGCAGCACCC AGTCCAGGCC CCTCTGCAGC AGCCGAGCAG TACTTTGACC 
CTGGGCCCCG TCCCCCAGAC TTCTCCACCT CAGGTGATTG GTCAAACAGC CTCTCTGCCC 
TCTACTGTTC AACCTATTGT CCCGCTGCAG TACCGTCAGC CACCTCAGGA TGGAATCACA 
CTACCTCAAT CCCTGCCCAG CGCTCAAGTC TTACAGATAT GGGCATGTCA ACTCAGCCCT 
CCTGTTTTCT CACCACCTCC AAATGCCGAA CAGCTCTATT TTCTATATCA GGCCTGTCAG 
GCACCACTAA GTGTGTCCCA ATCACAACCA GTAGAGCAGA CACAACCTCA AACATCTCTG 
GTTCCTGCCT CCACAGAAAG TGGACACTCT GATCCGGCAT CTGGTCTAAG CGATGGGAAT 
GAGGGGAGAC ACGAGGGTCG CTCTATGAAA CGACCCCAAC GCCGTTCTGT ACGCAGCCGC 
TCGCGTCATG AAAAAACAGC TAAGGTCAAG CTCAATGTGC TAAATATTTC TAATATTGGA 
GACAGAGTCG CTGAATGTCA GCTGGAAACC CACAACAGAA AGATGGTCAC ATTCAAGTTT 
GATCTGGATG GGGATAATCC TGTAGAAATC GCTGAAATTA TGGTCAAAAG TGATTTTATT 
TTGGAGAGTG AACGAGAGTC CTTCATAGAA CAGGTTCAAC TGGTCATTCA GAATGCAGAT 
CAGAAAGGAA AAGCTCCCCA AAATAGCCAA ACTCAGGTGA TCAGTAATTC GAGCCAACAA 
GTACCTGAAA TTTTAACATC CAGTGTGCCT GGTATGCCAC CTAGCTTAGC AGCACAGGTT 
GTACATTCAG CTGGCAGGCG CTTCATAGTC AGCCCCGTGC CTGAAGCACG ACTTCGGGAG 
CCGTTGTTTG GTACTCCCTC AGCAAACACA TCATTTGAAG ATACAGCACC AGCACCTGAT 
CCAACATCAG CCAGCAGTGC TCCGCAGGAC CAAGCCAACT TTGCCTCTGA TTCAAACCAA 
ATTTCTGTGG TACCACCGAC ACCTGGAGGT CTTGCCCCAG AGAGCTCCAT TCCACCTACT 
TCTCAATCAC CATACCAGAA TCCTGTCCCA TCATCCACTG CAACTTTGAC TGTGACCACC 
ACTCAAGTAA CCACTCCAAC ACCAACTACC ACTCCGCAAC CTATAACAGG GAGGACGTCT 
CCTTCACCAG CCCCAACAGA ACCACCAGCA GCACGTCGTA TGTCACTTCC CAGCCCATCC 
CAGACACCTC AAATTGCTCC AGGAGAGAGT AATGGCTTGG ATCAAGCACA AGCCTCAAAT 
CAGCAAGTAA CTCAACCGAG TGCAACCGGA GATAGTGAGA GCGATGTACA GGGGAAACCA 
CCTGGAATTG AAGACATTCA TGCTCTAGAC CAAAAGCTGC GCTCCCTTTT CAAGGACTCA 
TCCCAGAACT CTTGCAGCCA ACCTGACGGG TCTGGAGAGA CTTCCACAAC CTCTCCACCC 
AACACCATAA GCACTAACAC TCCTGGCTTT GTCTCTGGTG TGACGCCTTC AAGTTTGTCG 
CTCAATTCCA GCGGGAACTT TGCACCTGGT GCTTTCGCCT CCATTGGTAC TCCTACAAGC 
TACACCCAGA CACCATCTAC CGACCAGTCC CCTCCAAGGC CACAGATTTC TGTAGGTGTA 
ACTCAACCAG ATGGACACAA CAGTACTGAG GAGAAAGAAC CACAACTTGA GAAAGCTTTC 
CAACTTGGCC GATTTCAGGT GTCTGTGGCC AGTGATCAAG ACCCACCAAC TGCTCTAGAC 
CCAGGAGTGG TGGTTTCATC TTCCTCTGCT ACCCCATCTT CAACATCATC ATCATCTTCT 
TCTTCATCAC CATCATCTCT CTCTAGCCCC GAAAACACAA TTCATAAATC TCAGACTCCT 
CCACCCAGGG TCAGCCCTGA ACCCGTACCA CCTACCACTA CAGTTGGCCG CTTTCAGGTG 
ACCTCCAGCA CTGACATCAA ACTTGGTCGC TTCTCAGTGA CACCAGCAGA AAGAAAGTCT 
TCCTCAGTTG AGGGACCAGA CACCGCCAAA GAGCCTAAAT TGACCCCTTC CAGTGTGTCC 
ATTACCAATC ACTATCTCAG TAGCGACAAC GACTCAGAAC CAGAAGATGA GGGCTTAAGA 
CGAGAGATGA ACAGGCTCAA GGAGAGACAT AAGGTTGAGA TCATGGCATT GCAGAATAAA 
CAGAAAAAGG AAATCGAGGA GCTCTTCACT CGTCTGGGCA AAACACCATC TGTGGTTGTG 
CCACCCACTG TGGCCATGAC GCCTGGTAGA CGGAGACCTG CTAAGGGCAA GGGGAACAGG 
CCAAGAAGTG GTAGTGCCAC TCAGGCTGCA TCTTTTCAAC AAGGGAACAA ACAAGCTGTG 
CAGAGTGGTT CACTCAGTCA GTCAGCCAGT GAGAAGACTT CACCTTCAGA AACAGCACCA 
AGCTCAAACA ATGATGTGCA AGATAATACA AGTCCAGTGA GAAAAGAAAA CGGACAGAGC 
CATGACCAGG ACCAAACACC GACAACTAAT CTGCCACGCA AGGGCACATT CACAGATGAC 
CTCCATCAGC TGGTTGATAA CTGGGCCAGA GATGCCATGA ACATGATGCC ACAGGTCAAG 
AAAGGATCCA AAAAGAATGT TCATGAAATG CCACGAAAAT ACTCCGCACC AGGTCAGCTT 
TGTTCCACTG GTGGCCACAT CCCAGCGACC ATCAACCCCA CAGCTGGACG GAAGGGTTCA 
ATAGGTGTGG CCAATCAGCA ATTTGGCTAC ACTTGTCCCA ACTACAACAT GCCTCAATGG 
AATCAGGTGG GGACAATATC ATCCACCGCT CCACCTGCAG GACTACAGCA GGGTTTCCTT 
ATCCCATCAG GAGCCCACCA AACCGGAAAT AACTGCGGAT CAAGCAATCT GCGCACCACT 
CAGGCAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1a ( XM_009298027.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296302.2 Danio rerio WNK lysine deficient protein kinase 1a (wnk1a), transcript variant X1, mRNA.

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CloneID	ODa146839
Clone ID Related Accession (Same CDS sequence)	XM_009298027.3
Accession Version	XM_009298027.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4902bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395370.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009298027.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAAA ATCTCAACGA CAAGATGGTC AAGTTCCTTT CCCCCCCTTC GAAGAACACC 
AACGGCTCCA GCTCAGACAC TCTGGTGGGT GAGCATCTAG GTGTCGATGT CCGTCGTCGC 
CACCACACCA TGGATCGAGA ACTGCTGAAG GCAGAGCACC GTTTCTTTCG CCGCAGCGTC 
ATCAACGACT CCAATGCTAC AGCGCTTGAG CTGCCCAGCA AGAATGCAAT CCTAACCCAC 
TCCACTGACT CCCATGCCCC TGTCTGTGAA CCTCCTGCCC TAGAAACCAA AGCCGCTTCA 
GTGATCACAC AATCTGCACC AGAAAAAAAA GTGGCTGATG TTGTTGAGAT GGTTATTGAG 
TCAACACCAG TGTTGATTAC AAACGCTGCA CTGCATCTTT CTGCAGAAGC ACCTAGTTTG 
GTTTCAGAGG TGAAGTCTGG AGATAGCATT GATGGAAAAC GAGAGGTTGA GAAAGAGGAG 
GATGAAGATA GTAAAGAAAA GGCGGCACGT GCTGAGGCTG AGTTGCGGGA TGCTAAGAAG 
GAGGAGGAAG ACAATGAGGA AATTGAGACT AACGCTGTTG GGACATCACC GGATGGCCGC 
TTCTTGAAGT TTGACATCGA GATAGGACGT GGCTCTTTTA AGACGGTCTA CAAGGGACTG 
GATACGGAAA CTACAGTGGA GGTTGCATGG TGTGAGTTAC AGGATCGCAA GTTGTCCAAG 
TCAGAGCGGC AGCGTTTTAA AGAGGAAGCT GGAATGTTGA AGGGTCTTCA GCATCCCAAC 
ATAGTACGCT TTTATGACTC CTGGGAATCA TCCTTGAAAG GGAGGAAGTG TATTGTCTTG 
GTGACTGAAC TCATGACATC TGGGACACTC AAGACGTATC TAAAACGGTT TAAGGAGATG 
AAGATCAAAG TCTTGCGCAG CTGGTGCCGG CAGATTTTGA AGGGCCTACA CTTCCTTCAT 
ACCAGGTCAC CACCTATCAT CCACCGGGAT CTGAAGTGTG ACAACATCTT CATCACTGGG 
CCTACTGGAT CAGTCAAGAT TGGAGATTTG GGCCTTGCGA CCCTCAAGAG GGCCTCCTTT 
GCTAAGAGCG TCATAGGTAC CCCTGAGTTC ATGGCGCCTG AGATGTATGA AGAGAAGTAT 
GACGAGTCGG TGGATGTGTA TGCCTTTGGG ATGTGTATGC TGGAGATGGC TACTTCTGAG 
TACCCGTACT CCGAGTGTCA GAATGCAGCT CAGATCTATC GCAGAGTCAC CAGCGGGGTG 
AAGCCTGGCA GTTTTGACAA AGTGGCTATT CCAGAGGTGA AGGAGATCAT TGAGGGTTGC 
ATTCGTCAGA ATAAAGATGA GAGGTACTGC ATCAAGGATC TCCTAAGCCA TGCCTTCTTC 
CAGGAGGAGA CTGGAGTTCG TGTAGAACTG GCTGAGGAGG ATGATGGAGA GCTTGTGGCT 
ATAAAGTTGT TGCTGCGCAT TGAGGATGTT AAGAAACTTA AAGGCAAATA CAAAGAGAAT 
GAAGCCATCG AGTTTTCCTT CGACTTGGAT AAAGACAACC CAGATGATGT GGCTCAAGAA 
ATGGTTGAAT CAGGCTACAT TTGTGAGGGT GACCACAAAA CCATTGCAAA GGCCATAAAG 
GACAGAGTGT CCCTGATCTC CCGTAAGAGA GAGCAGAGAA AACTGGTGAG AGAAGAACAG 
GAAAAAAGGA AGATGGAGCA GGAGAATGAC GCCGCCCAGC AAACTAACCC CACAGTCAAG 
TCCCCTGGTT CTGCTCTGGC CTCGGTCATA ATGGAGTCTG AAGAAGCAGA AACAGACCAG 
CACCAGCAGC CTGGAGCCTC TGCTTCTGCT GTAGGGTTTG TGGATCCTCA AAGTTCAGTG 
ACCATCCTCG AGGCTCATCC TGTCCAGCCA AACACATCTT TCAGCACCAC ACAGCCTGAT 
CCGCAGCCAC AGCACACAAT TATACGTCCA CAAAGCATGT CGCAGCACCC AGTCCAGGCC 
CCTCTGCAGC AGCCGAGCAG TACTTTGACC CTGGGCCCCG TCCCCCAGAC TTCTCCACCT 
CAGGTGATTG GTCAAACAGC CTCTCTGCCC TCTACTGTTC AACCTATTGT CCCGCTGCAG 
TACCGTCAGC CACCTCAGGA TGGAATCACA CTACCTCAAT CCCTGCCCAG CGCTCAAGTC 
TTACAGATAT GGGCATGTCA ACTCAGCCCT CCTGTTTTCT CACCACCTCC AAATGCCGAA 
CAGCTCTATT TTCTATATCA GGCCTGTCAG GCACCACTAA GTGTGTCCCA ATCACAACCA 
GTAGAGCAGA CACAACCTCA AACATCTCTG GTTCCTGCCT CCACAGAAAG TGGACACTCT 
GATCCGGCAT CTGGTCTAAG CGATGGGAAT GAGGGGAGAC ACGAGGGTCG CTCTATGAAA 
CGACCCCAAC GCCGTTCTGT ACGCAGCCGC TCGCGTCATG AAAAAACAGC TAAGGTCAAG 
CTCAATGTGC TAAATATTTC TAATATTGGA GACAGAGTCG CTGAATGTCA GCTGGAAACC 
CACAACAGAA AGATGGTCAC ATTCAAGTTT GATCTGGATG GGGATAATCC TGTAGAAATC 
GCTGAAATTA TGGTCAAAAG TGATTTTATT TTGGAGAGTG AACGAGAGTC CTTCATAGAA 
CAGGTTCAAC TGGTCATTCA GAATGCAGAT CAGAAAGGAA AAGCTCCCCA AAATAGCCAA 
ACTCAGGTGA TCAGTAATTC GAGCCAACAA GTACCTGAAA TTTTAACATC CAGTGTGCCT 
GGTATGCCAC CTAGCTTAGC AGCACAGGTT GTACATTCAG CTGGCAGGCG CTTCATAGTC 
AGCCCCGTGC CTGAAGCACG ACTTCGGGAG CCGTTGTTTG GTACTCCCTC AGCAAACACA 
TCATTTGAAG ATACAGCACC AGCACCTGAT CCAACATCAG CCAGCAGTGC TCCGCAGGAC 
CAAGCCAACT TTGCCTCTGA TTCAAACCAA ATTTCTGTGG TACCACCGAC ACCTGGAGGT 
CTTGCCCCAG AGAGCTCCAT TCCACCTACT TCTCAATCAC CATACCAGAA TCCTGTCCCA 
TCATCCACTG CAACTTTGAC TGTGACCACC ACTCAAGTAA CCACTCCAAC ACCAACTACC 
ACTCCGCAAC CTATAACAGG GAGGACGTCT CCTTCACCAG CCCCAACAGA ACCACCAGCA 
GCACGTCGTA TGTCACTTCC CAGCCCATCC CAGACACCTC AAATTGCTCC AGGAGAGAGT 
AATGGCTTGG ATCAAGCACA AGCCTCAAAT CAGCAAGTAA CTCAACCGAG TGCAACCGGA 
GATAGTGAGA GCGATGTACA GGGGAAACCA CCTGGAATTG AAGACATTCA TGCTCTAGAC 
CAAAAGCTGC GCTCCCTTTT CAAGGACTCA TCCCAGAACT CTTGCAGCCA ACCTGACGGG 
TCTGGAGAGA CTTCCACAAC CTCTCCACCC AACACCATAA GCACTAACAC TCCTGGCTTT 
GTCTCTGGTG TGACGCCTTC AAGTTTGTCG CTCAATTCCA GCGGGAACTT TGCACCTGGT 
GCTTTCGCCT CCATTGGTAC TCCTACAAGC TACACCCAGA CACCATCTAC CGACCAGTCC 
CCTCCAAGGC CACAGATTTC TGTAGGTGTA ACTCAACCAG ATGGACACAA CAGTACTGAG 
GAGAAAGAAC CACAACTTGA GAAAGCTTTC CAACTTGGCC GATTTCAGGT GTCTGTGGCC 
AGTGATCAAG ACCCACCAAC TGCTCTAGAC CCAGGAGTGG TGGTTTCATC TTCCTCTGCT 
ACCCCATCTT CAACATCATC ATCATCTTCT TCTTCATCAC CATCATCTCT CTCTAGCCCC 
GAAAACACAA TTCATAAATC TCAGACTCCT CCACCCAGGG TCAGCCCTGA ACCCGTACCA 
CCTACCACTA CAGTTGGCCG CTTTCAGGTG ACCTCCAGCA CTGACATCAA ACTTGGTCGC 
TTCTCAGTGA CACCAGCAGA AAGAAAGTCT TCCTCAGTTG AGGGACCAGA CACCGCCAAA 
GAGCCTAAAT TGACCCCTTC CAGTGTGTCC ATTACCAATC ACTATCTCAG TAGCGACAAC 
GACTCAGAAC CAGAAGATGA GGGCTTAAGA CGAGAGATGA ACAGGCTCAA GGAGAGACAT 
AAGGTTGAGA TCATGGCATT GCAGAATAAA CAGAAAAAGG AAATCGAGGA GCTCTTCACT 
CGTCTGGGCA AAACACCATC TGTGGTTGTG CCACCCACTG TGGCCATGAC GCCTGGTAGA 
CGGAGACCTG CTAAGGGCAA GGGGAACAGG CCAAGAAGTG GTAGTGCCAC TCAGGCTGCA 
TCTTTTCAAC AAGGGAACAA ACAAGCTGTG CAGAGTGGTT CACTCAGTCA GTCAGCCAGT 
GAGAAGACTT CACCTTCAGA AACAGCACCA AGCTCAAACA ATGATGTGCA AGATAATACA 
AGTCCAGTGA GAAAAGAAAA CGGACAGAGC CATGACCAGG ACCAAACACC GACAACTAAT 
CTGCCACGCA AGGGCACATT CACAGATGAC CTCCATCAGC TGGTTGATAA CTGGGCCAGA 
GATGCCATGA ACATGATGCC ACAGGTCAAG AAAGGATCCA AAAAGAATGT TCATGAAATG 
CCACGAAAAT ACTCCGCACC AGGTCAGCTT TGTTCCACTG GTGGCCACAT CCCAGCGACC 
ATCAACCCCA CAGCTGGACG GAAGGGTTCA ATAGGTGTGG CCAATCAGCA ATTTGGCTAC 
ACTTGTCCCA ACTACAACAT GCCTCAATGG AATCAGGTGG GGACAATATC ATCCACCGCT 
CCACCTGCAG GACTACAGCA GGGTTTCCTT ATCCCATCAG GAGCCCACCA AACCGGAAAT 
AACTGCGGAT CAAGCAATCT GCGCACCACT CAGGCAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296302.2
CDS	1190..6091
Translation	MSENLNDKMVKFLSPPSKNTNGSSSDTLVGEHLGVDVRRRHHTMDRELLKAEHRFFRRSVINDSNATALELPSKNAILTHSTDSHAPVCEPPALETKAASVITQSAPEKKVADVVEMVIESTPVLITNAALHLSAEAPSLVSEVKSGDSIDGKREVEKEEDEDSKEKAARAEAELRDAKKEEEDNEEIETNAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSKSERQRFKEEAGMLKGLQHPNIVRFYDSWESSLKGRKCIVLVTELMTSGTLKTYLKRFKEMKIKVLRSWCRQILKGLHFLHTRSPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYCIKDLLSHAFFQEETGVRVELAEEDDGELVAIKLLLRIEDVKKLKGKYKENEAIEFSFDLDKDNPDDVAQEMVESGYICEGDHKTIAKAIKDRVSLISRKREQRKLVREEQEKRKMEQENDAAQQTNPTVKSPGSALASVIMESEEAETDQHQQPGASASAVGFVDPQSSVTILEAHPVQPNTSFSTTQPDPQPQHTIIRPQSMSQHPVQAPLQQPSSTLTLGPVPQTSPPQVIGQTASLPSTVQPIVPLQYRQPPQDGITLPQSLPSAQVLQIWACQLSPPVFSPPPNAEQLYFLYQACQAPLSVSQSQPVEQTQPQTSLVPASTESGHSDPASGLSDGNEGRHEGRSMKRPQRRSVRSRSRHEKTAKVKLNVLNISNIGDRVAECQLETHNRKMVTFKFDLDGDNPVEIAEIMVKSDFILESERESFIEQVQLVIQNADQKGKAPQNSQTQVISNSSQQVPEILTSSVPGMPPSLAAQVVHSAGRRFIVSPVPEARLREPLFGTPSANTSFEDTAPAPDPTSASSAPQDQANFASDSNQISVVPPTPGGLAPESSIPPTSQSPYQNPVPSSTATLTVTTTQVTTPTPTTTPQPITGRTSPSPAPTEPPAARRMSLPSPSQTPQIAPGESNGLDQAQASNQQVTQPSATGDSESDVQGKPPGIEDIHALDQKLRSLFKDSSQNSCSQPDGSGETSTTSPPNTISTNTPGFVSGVTPSSLSLNSSGNFAPGAFASIGTPTSYTQTPSTDQSPPRPQISVGVTQPDGHNSTEEKEPQLEKAFQLGRFQVSVASDQDPPTALDPGVVVSSSSATPSSTSSSSSSSSPSSLSSPENTIHKSQTPPPRVSPEPVPPTTTVGRFQVTSSTDIKLGRFSVTPAERKSSSVEGPDTAKEPKLTPSSVSITNHYLSSDNDSEPEDEGLRREMNRLKERHKVEIMALQNKQKKEIEELFTRLGKTPSVVVPPTVAMTPGRRRPAKGKGNRPRSGSATQAASFQQGNKQAVQSGSLSQSASEKTSPSETAPSSNNDVQDNTSPVRKENGQSHDQDQTPTTNLPRKGTFTDDLHQLVDNWARDAMNMMPQVKKGSKKNVHEMPRKYSAPGQLCSTGGHIPATINPTAGRKGSIGVANQQFGYTCPNYNMPQWNQVGTISSTAPPAGLQQGFLIPSGAHQTGNNCGSSNLRTTQAS

Target ORF information:

RefSeq Version	XM_009298027.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1a (wnk1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298027.3

ORF Insert Sequence:

ATGTCAGAAA ATCTCAACGA CAAGATGGTC AAGTTCCTTT CCCCCCCTTC GAAGAACACC 
AACGGCTCCA GCTCAGACAC TCTGGTGGGT GAGCATCTAG GTGTCGATGT CCGTCGTCGC 
CACCACACCA TGGATCGAGA ACTGCTGAAG GCAGAGCACC GTTTCTTTCG CCGCAGCGTC 
ATCAACGACT CCAATGCTAC AGCGCTTGAG CTGCCCAGCA AGAATGCAAT CCTAACCCAC 
TCCACTGACT CCCATGCCCC TGTCTGTGAA CCTCCTGCCC TAGAAACCAA AGCCGCTTCA 
GTGATCACAC AATCTGCACC AGAAAAAAAA GTGGCTGATG TTGTTGAGAT GGTTATTGAG 
TCAACACCAG TGTTGATTAC AAACGCTGCA CTGCATCTTT CTGCAGAAGC ACCTAGTTTG 
GTTTCAGAGG TGAAGTCTGG AGATAGCATT GATGGAAAAC GAGAGGTTGA GAAAGAGGAG 
GATGAAGATA GTAAAGAAAA GGCGGCACGT GCTGAGGCTG AGTTGCGGGA TGCTAAGAAG 
GAGGAGGAAG ACAATGAGGA AATTGAGACT AACGCTGTTG GGACATCACC GGATGGCCGC 
TTCTTGAAGT TTGACATCGA GATAGGACGT GGCTCTTTTA AGACGGTCTA CAAGGGACTG 
GATACGGAAA CTACAGTGGA GGTTGCATGG TGTGAGTTAC AGGATCGCAA GTTGTCCAAG 
TCAGAGCGGC AGCGTTTTAA AGAGGAAGCT GGAATGTTGA AGGGTCTTCA GCATCCCAAC 
ATAGTACGCT TTTATGACTC CTGGGAATCA TCCTTGAAAG GGAGGAAGTG TATTGTCTTG 
GTGACTGAAC TCATGACATC TGGGACACTC AAGACGTATC TAAAACGGTT TAAGGAGATG 
AAGATCAAAG TCTTGCGCAG CTGGTGCCGG CAGATTTTGA AGGGCCTACA CTTCCTTCAT 
ACCAGGTCAC CACCTATCAT CCACCGGGAT CTGAAGTGTG ACAACATCTT CATCACTGGG 
CCTACTGGAT CAGTCAAGAT TGGAGATTTG GGCCTTGCGA CCCTCAAGAG GGCCTCCTTT 
GCTAAGAGCG TCATAGGTAC CCCTGAGTTC ATGGCGCCTG AGATGTATGA AGAGAAGTAT 
GACGAGTCGG TGGATGTGTA TGCCTTTGGG ATGTGTATGC TGGAGATGGC TACTTCTGAG 
TACCCGTACT CCGAGTGTCA GAATGCAGCT CAGATCTATC GCAGAGTCAC CAGCGGGGTG 
AAGCCTGGCA GTTTTGACAA AGTGGCTATT CCAGAGGTGA AGGAGATCAT TGAGGGTTGC 
ATTCGTCAGA ATAAAGATGA GAGGTACTGC ATCAAGGATC TCCTAAGCCA TGCCTTCTTC 
CAGGAGGAGA CTGGAGTTCG TGTAGAACTG GCTGAGGAGG ATGATGGAGA GCTTGTGGCT 
ATAAAGTTGT TGCTGCGCAT TGAGGATGTT AAGAAACTTA AAGGCAAATA CAAAGAGAAT 
GAAGCCATCG AGTTTTCCTT CGACTTGGAT AAAGACAACC CAGATGATGT GGCTCAAGAA 
ATGGTTGAAT CAGGCTACAT TTGTGAGGGT GACCACAAAA CCATTGCAAA GGCCATAAAG 
GACAGAGTGT CCCTGATCTC CCGTAAGAGA GAGCAGAGAA AACTGGTGAG AGAAGAACAG 
GAAAAAAGGA AGATGGAGCA GGAGAATGAC GCCGCCCAGC AAACTAACCC CACAGTCAAG 
TCCCCTGGTT CTGCTCTGGC CTCGGTCATA ATGGAGTCTG AAGAAGCAGA AACAGACCAG 
CACCAGCAGC CTGGAGCCTC TGCTTCTGCT GTAGGGTTTG TGGATCCTCA AAGTTCAGTG 
ACCATCCTCG AGGCTCATCC TGTCCAGCCA AACACATCTT TCAGCACCAC ACAGCCTGAT 
CCGCAGCCAC AGCACACAAT TATACGTCCA CAAAGCATGT CGCAGCACCC AGTCCAGGCC 
CCTCTGCAGC AGCCGAGCAG TACTTTGACC CTGGGCCCCG TCCCCCAGAC TTCTCCACCT 
CAGGTGATTG GTCAAACAGC CTCTCTGCCC TCTACTGTTC AACCTATTGT CCCGCTGCAG 
TACCGTCAGC CACCTCAGGA TGGAATCACA CTACCTCAAT CCCTGCCCAG CGCTCAAGTC 
TTACAGATAT GGGCATGTCA ACTCAGCCCT CCTGTTTTCT CACCACCTCC AAATGCCGAA 
CAGCTCTATT TTCTATATCA GGCCTGTCAG GCACCACTAA GTGTGTCCCA ATCACAACCA 
GTAGAGCAGA CACAACCTCA AACATCTCTG GTTCCTGCCT CCACAGAAAG TGGACACTCT 
GATCCGGCAT CTGGTCTAAG CGATGGGAAT GAGGGGAGAC ACGAGGGTCG CTCTATGAAA 
CGACCCCAAC GCCGTTCTGT ACGCAGCCGC TCGCGTCATG AAAAAACAGC TAAGGTCAAG 
CTCAATGTGC TAAATATTTC TAATATTGGA GACAGAGTCG CTGAATGTCA GCTGGAAACC 
CACAACAGAA AGATGGTCAC ATTCAAGTTT GATCTGGATG GGGATAATCC TGTAGAAATC 
GCTGAAATTA TGGTCAAAAG TGATTTTATT TTGGAGAGTG AACGAGAGTC CTTCATAGAA 
CAGGTTCAAC TGGTCATTCA GAATGCAGAT CAGAAAGGAA AAGCTCCCCA AAATAGCCAA 
ACTCAGGTGA TCAGTAATTC GAGCCAACAA GTACCTGAAA TTTTAACATC CAGTGTGCCT 
GGTATGCCAC CTAGCTTAGC AGCACAGGTT GTACATTCAG CTGGCAGGCG CTTCATAGTC 
AGCCCCGTGC CTGAAGCACG ACTTCGGGAG CCGTTGTTTG GTACTCCCTC AGCAAACACA 
TCATTTGAAG ATACAGCACC AGCACCTGAT CCAACATCAG CCAGCAGTGC TCCGCAGGAC 
CAAGCCAACT TTGCCTCTGA TTCAAACCAA ATTTCTGTGG TACCACCGAC ACCTGGAGGT 
CTTGCCCCAG AGAGCTCCAT TCCACCTACT TCTCAATCAC CATACCAGAA TCCTGTCCCA 
TCATCCACTG CAACTTTGAC TGTGACCACC ACTCAAGTAA CCACTCCAAC ACCAACTACC 
ACTCCGCAAC CTATAACAGG GAGGACGTCT CCTTCACCAG CCCCAACAGA ACCACCAGCA 
GCACGTCGTA TGTCACTTCC CAGCCCATCC CAGACACCTC AAATTGCTCC AGGAGAGAGT 
AATGGCTTGG ATCAAGCACA AGCCTCAAAT CAGCAAGTAA CTCAACCGAG TGCAACCGGA 
GATAGTGAGA GCGATGTACA GGGGAAACCA CCTGGAATTG AAGACATTCA TGCTCTAGAC 
CAAAAGCTGC GCTCCCTTTT CAAGGACTCA TCCCAGAACT CTTGCAGCCA ACCTGACGGG 
TCTGGAGAGA CTTCCACAAC CTCTCCACCC AACACCATAA GCACTAACAC TCCTGGCTTT 
GTCTCTGGTG TGACGCCTTC AAGTTTGTCG CTCAATTCCA GCGGGAACTT TGCACCTGGT 
GCTTTCGCCT CCATTGGTAC TCCTACAAGC TACACCCAGA CACCATCTAC CGACCAGTCC 
CCTCCAAGGC CACAGATTTC TGTAGGTGTA ACTCAACCAG ATGGACACAA CAGTACTGAG 
GAGAAAGAAC CACAACTTGA GAAAGCTTTC CAACTTGGCC GATTTCAGGT GTCTGTGGCC 
AGTGATCAAG ACCCACCAAC TGCTCTAGAC CCAGGAGTGG TGGTTTCATC TTCCTCTGCT 
ACCCCATCTT CAACATCATC ATCATCTTCT TCTTCATCAC CATCATCTCT CTCTAGCCCC 
GAAAACACAA TTCATAAATC TCAGACTCCT CCACCCAGGG TCAGCCCTGA ACCCGTACCA 
CCTACCACTA CAGTTGGCCG CTTTCAGGTG ACCTCCAGCA CTGACATCAA ACTTGGTCGC 
TTCTCAGTGA CACCAGCAGA AAGAAAGTCT TCCTCAGTTG AGGGACCAGA CACCGCCAAA 
GAGCCTAAAT TGACCCCTTC CAGTGTGTCC ATTACCAATC ACTATCTCAG TAGCGACAAC 
GACTCAGAAC CAGAAGATGA GGGCTTAAGA CGAGAGATGA ACAGGCTCAA GGAGAGACAT 
AAGGTTGAGA TCATGGCATT GCAGAATAAA CAGAAAAAGG AAATCGAGGA GCTCTTCACT 
CGTCTGGGCA AAACACCATC TGTGGTTGTG CCACCCACTG TGGCCATGAC GCCTGGTAGA 
CGGAGACCTG CTAAGGGCAA GGGGAACAGG CCAAGAAGTG GTAGTGCCAC TCAGGCTGCA 
TCTTTTCAAC AAGGGAACAA ACAAGCTGTG CAGAGTGGTT CACTCAGTCA GTCAGCCAGT 
GAGAAGACTT CACCTTCAGA AACAGCACCA AGCTCAAACA ATGATGTGCA AGATAATACA 
AGTCCAGTGA GAAAAGAAAA CGGACAGAGC CATGACCAGG ACCAAACACC GACAACTAAT 
CTGCCACGCA AGGGCACATT CACAGATGAC CTCCATCAGC TGGTTGATAA CTGGGCCAGA 
GATGCCATGA ACATGATGCC ACAGGTCAAG AAAGGATCCA AAAAGAATGT TCATGAAATG 
CCACGAAAAT ACTCCGCACC AGGTCAGCTT TGTTCCACTG GTGGCCACAT CCCAGCGACC 
ATCAACCCCA CAGCTGGACG GAAGGGTTCA ATAGGTGTGG CCAATCAGCA ATTTGGCTAC 
ACTTGTCCCA ACTACAACAT GCCTCAATGG AATCAGGTGG GGACAATATC ATCCACCGCT 
CCACCTGCAG GACTACAGCA GGGTTTCCTT ATCCCATCAG GAGCCCACCA AACCGGAAAT 
AACTGCGGAT CAAGCAATCT GCGCACCACT CAGGCAAGCT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1a ( XM_002666846.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_002666892.4 Danio rerio WNK lysine deficient protein kinase 1a (wnk1a), transcript variant X2, mRNA.

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CloneID	ODa146840
Clone ID Related Accession (Same CDS sequence)	XM_002666846.6
Accession Version	XM_002666846.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395370.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_002666846.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAAA ATCTCAACGA CAAGATGGTC AAGTTCCTTT CCCCCCCTTC GAAGAACACC 
AACGGCTCCA GCTCAGACAC TCTGGTGGGT GAGCATCTAG GTGTCGATGT CCGTCGTCGC 
CACCACACCA TGGATCGAGA ACTGCTGAAG GCAGAGCACC GTTTCTTTCG CCGCAGCGTC 
ATCAACGACT CCAATGCTAC AGCGCTTGAG CTGCCCAGCA AGAATGCAAT CCTAACCCAC 
TCCACTGACT CCCATGCCCC TGTCTGTGAA CCTCCTGCCC TAGAAACCAA AGCCGCTTCA 
GTGATCACAC AATCTGCACC AGAAAAAAAA GTGGCTGATG TTGTTGAGAT GGTTATTGAG 
TCAACACCAG TGTTGATTAC AAACGCTGCA CTGCATCTTT CTGCAGAAGC ACCTAGTTTG 
GTTTCAGAGG TGAAGTCTGG AGATAGCATT GATGGAAAAC GAGAGGTTGA GAAAGAGGAG 
GATGAAGATA GTAAAGAAAA GGCGGCACGT GCTGAGGCTG AGTTGCGGGA TGCTAAGAAG 
GAGGAGGAAG ACAATGAGGA AATTGAGACT AACGCTGTTG GGACATCACC GGATGGCCGC 
TTCTTGAAGT TTGACATCGA GATAGGACGT GGCTCTTTTA AGACGGTCTA CAAGGGACTG 
GATACGGAAA CTACAGTGGA GGTTGCATGG TGTGAGTTAC AGGATCGCAA GTTGTCCAAG 
TCAGAGCGGC AGCGTTTTAA AGAGGAAGCT GGAATGTTGA AGGGTCTTCA GCATCCCAAC 
ATAGTACGCT TTTATGACTC CTGGGAATCA TCCTTGAAAG GGAGGAAGTG TATTGTCTTG 
GTGACTGAAC TCATGACATC TGGGACACTC AAGACGTATC TAAAACGGTT TAAGGAGATG 
AAGATCAAAG TCTTGCGCAG CTGGTGCCGG CAGATTTTGA AGGGCCTACA CTTCCTTCAT 
ACCAGGTCAC CACCTATCAT CCACCGGGAT CTGAAGTGTG ACAACATCTT CATCACTGGG 
CCTACTGGAT CAGTCAAGAT TGGAGATTTG GGCCTTGCGA CCCTCAAGAG GGCCTCCTTT 
GCTAAGAGCG TCATAGGTAC CCCTGAGTTC ATGGCGCCTG AGATGTATGA AGAGAAGTAT 
GACGAGTCGG TGGATGTGTA TGCCTTTGGG ATGTGTATGC TGGAGATGGC TACTTCTGAG 
TACCCGTACT CCGAGTGTCA GAATGCAGCT CAGATCTATC GCAGAGTCAC CAGCGGGGTG 
AAGCCTGGCA GTTTTGACAA AGTGGCTATT CCAGAGGTGA AGGAGATCAT TGAGGGTTGC 
ATTCGTCAGA ATAAAGATGA GAGGTACTGC ATCAAGGATC TCCTAAGCCA TGCCTTCTTC 
CAGGAGGAGA CTGGAGTTCG TGTAGAACTG GCTGAGGAGG ATGATGGAGA GCTTGTGGCT 
ATAAAGTTGT TGCTGCGCAT TGAGGATGTT AAGAAACTTA AAGGCAAATA CAAAGAGAAT 
GAAGCCATCG AGTTTTCCTT CGACTTGGAT AAAGACAACC CAGATGATGT GGCTCAAGAA 
ATGGTTGAAT CAGGCTACAT TTGTGAGGGT GACCACAAAA CCATTGCAAA GGCCATAAAG 
GACAGAGTGT CCCTGATCTC CCGTAAGAGA GAGCAGAGAA AACTGGTGAG AGAAGAACAG 
GAAAAAAGGA AGATGGAGCA GGAGAATGAC GCCGCCCAGC AAACTAACCC CACAGTCAAG 
TCCCCTGGTT CTGCTCTGGC CTCGGTCATA ATGGAGTCTG AAGAAGCAGA AACAGACCAG 
CACCAGCAGC CTGGAGCCTC TGCTTCTGCT GTAGGGTTTG TGGATCCTCA AAGTTCAGTG 
ACCATCCTCG AGGCTCATCC TGTCCAGCCA AACACATCTT TCAGCACCAC ACAGCCTGAT 
CCGCAGCCAC AGCACACAAT TATACGTCCA CAAAGCATGT CGCAGCACCC AGTCCAGGCC 
CCTCTGCAGC AGCCGAGCAG TACTTTGACC CTGGGCCCCG TCCCCCAGAC TTCTCCACCT 
CAGGTGATTG GTCAAACAGC CTCTCTGCCC TCTACTGTTC AACCTATTGT CCCGCTGCAG 
TACCGTCAGC CACCTCAGGA TGGAATCACA CTACCTCAAT CCCTGCCCAG CGCTCAAGTC 
TTACAGGCAC CACTAAGTGT GTCCCAATCA CAACCAGTAG AGCAGACACA ACCTCAAACA 
TCTCTGGTTC CTGCCTCCAC AGAAAGTGGA CACTCTGATC CGGCATCTGG TCTAAGCGAT 
GGGAATGAGG GGAGACACGA GGGTCGCTCT ATGAAACGAC CCCAACGCCG TTCTGTACGC 
AGCCGCTCGC GTCATGAAAA AACAGCTAAG GTCAAGCTCA ATGTGCTAAA TATTTCTAAT 
ATTGGAGACA GAGTCGCTGA ATGTCAGCTG GAAACCCACA ACAGAAAGAT GGTCACATTC 
AAGTTTGATC TGGATGGGGA TAATCCTGTA GAAATCGCTG AAATTATGGT CAAAAGTGAT 
TTTATTTTGG AGAGTGAACG AGAGTCCTTC ATAGAACAGG TTCAACTGGT CATTCAGAAT 
GCAGATCAGA AAGGAAAAGC TCCCCAAAAT AGCCAAACTC AGGTGATCAG TAATTCGAGC 
CAACAAGTAC CTGAAATTTT AACATCCAGT GTGCCTGGTA TGCCACCTAG CTTAGCAGCA 
CAGGTTGTAC ATTCAGCTGG CAGGCGCTTC ATAGTCAGCC CCGTGCCTGA AGCACGACTT 
CGGGAGCCGT TGTTTGGTAC TCCCTCAGCA AACACATCAT TTGAAGATAC AGCACCAGCA 
CCTGATCCAA CATCAGCCAG CAGTGCTCCG CAGGACCAAG CCAACTTTGC CTCTGATTCA 
AACCAAATTT CTGTGGTACC ACCGACACCT GGAGGTCTTG CCCCAGAGAG CTCCATTCCA 
CCTACTTCTC AATCACCATA CCAGAATCCT GTCCCATCAT CCACTGCAAC TTTGACTGTG 
ACCACCACTC AAGTAACCAC TCCAACACCA ACTACCACTC CGCAACCTAT AACAGGGAGG 
ACGTCTCCTT CACCAGCCCC AACAGAACCA CCAGCAGCAC GTCGTATGTC ACTTCCCAGC 
CCATCCCAGA CACCTCAAAT TGCTCCAGGA GAGAGTAATG GCTTGGATCA AGCACAAGCC 
TCAAATCAGC AAGTAACTCA ACCGAGTGCA ACCGGAGATA GTGAGAGCGA TGTACAGGGG 
AAACCACCTG GAATTGAAGA CATTCATGCT CTAGACCAAA AGCTGCGCTC CCTTTTCAAG 
GACTCATCCC AGAACTCTTG CAGCCAACCT GACGGGTCTG GAGAGACTTC CACAACCTCT 
CCACCCAACA CCATAAGCAC TAACACTCCT GGCTTTGTCT CTGGTGTGAC GCCTTCAAGT 
TTGTCGCTCA ATTCCAGCGG GAACTTTGCA CCTGGTGCTT TCGCCTCCAT TGGTACTCCT 
ACAAGCTACA CCCAGACACC ATCTACCGAC CAGTCCCCTC CAAGGCCACA GATTTCTGTA 
GGTGTAACTC AACCAGATGG ACACAACAGT ACTGAGGAGA AAGAACCACA ACTTGAGAAA 
GCTTTCCAAC TTGGCCGATT TCAGGTGTCT GTGGCCAGTG ATCAAGACCC ACCAACTGCT 
CTAGACCCAG GAGTGGTGGT TTCATCTTCC TCTGCTACCC CATCTTCAAC ATCATCATCA 
TCTTCTTCTT CATCACCATC ATCTCTCTCT AGCCCCGAAA ACACAATTCA TAAATCTCAG 
ACTCCTCCAC CCAGGGTCAG CCCTGAACCC GTACCACCTA CCACTACAGT TGGCCGCTTT 
CAGGTGACCT CCAGCACTGA CATCAAACTT GGTCGCTTCT CAGTGACACC AGCAGAAAGA 
AAGTCTTCCT CAGTTGAGGG ACCAGACACC GCCAAAGAGC CTAAATTGAC CCCTTCCAGT 
GTGTCCATTA CCAATCACTA TCTCAGTAGC GACAACGACT CAGAACCAGA AGATGAGGGC 
TTAAGACGAG AGATGAACAG GCTCAAGGAG AGACATAAGG TTGAGATCAT GGCATTGCAG 
AATAAACAGA AAAAGGAAAT CGAGGAGCTC TTCACTCGTC TGGGCAAAAC ACCATCTGTG 
GTTGTGCCAC CCACTGTGGC CATGACGCCT GGTAGACGGA GACCTGCTAA GGGCAAGGGG 
AACAGGCCAA GAAGTGGTAG TGCCACTCAG GCTGCATCTT TTCAACAAGG GAACAAACAA 
GCTGTGCAGA GTGGTTCACT CAGTCAGTCA GCCAGTGAGA AGACTTCACC TTCAGAAACA 
GCACCAAGCT CAAACAATGA TGTGCAAGAT AATACAAGTC CAGTGAGAAA AGAAAACGGA 
CAGAGCCATG ACCAGGACCA AACACCGACA ACTAATCTGC CACGCAAGGG CACATTCACA 
GATGACCTCC ATCAGCTGGT TGATAACTGG GCCAGAGATG CCATGAACAT GATGCCACAG 
GTCAAGAAAG GATCCAAAAA GAATGTTCAT GAAATGCCAC GAAAATACTC CGCACCAGGT 
CAGCTTTGTT CCACTGGTGG CCACATCCCA GCGACCATCA ACCCCACAGC TGGACGGAAG 
GGTTCAATAG GTGTGGCCAA TCAGCAATTT GGCTACACTT GTCCCAACTA CAACATGCCT 
CAATGGAATC AGGTGGGGAC AATATCATCC ACCGCTCCAC CTGCAGGACT ACAGCAGGGT 
TTCCTTATCC CATCAGGAGC CCACCAAACC GGAAATAACT GCGGATCAAG CAATCTGCGC 
ACCACTCAGG CAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002666892.4
CDS	1189..6006
Translation	MSENLNDKMVKFLSPPSKNTNGSSSDTLVGEHLGVDVRRRHHTMDRELLKAEHRFFRRSVINDSNATALELPSKNAILTHSTDSHAPVCEPPALETKAASVITQSAPEKKVADVVEMVIESTPVLITNAALHLSAEAPSLVSEVKSGDSIDGKREVEKEEDEDSKEKAARAEAELRDAKKEEEDNEEIETNAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSKSERQRFKEEAGMLKGLQHPNIVRFYDSWESSLKGRKCIVLVTELMTSGTLKTYLKRFKEMKIKVLRSWCRQILKGLHFLHTRSPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYCIKDLLSHAFFQEETGVRVELAEEDDGELVAIKLLLRIEDVKKLKGKYKENEAIEFSFDLDKDNPDDVAQEMVESGYICEGDHKTIAKAIKDRVSLISRKREQRKLVREEQEKRKMEQENDAAQQTNPTVKSPGSALASVIMESEEAETDQHQQPGASASAVGFVDPQSSVTILEAHPVQPNTSFSTTQPDPQPQHTIIRPQSMSQHPVQAPLQQPSSTLTLGPVPQTSPPQVIGQTASLPSTVQPIVPLQYRQPPQDGITLPQSLPSAQVLQAPLSVSQSQPVEQTQPQTSLVPASTESGHSDPASGLSDGNEGRHEGRSMKRPQRRSVRSRSRHEKTAKVKLNVLNISNIGDRVAECQLETHNRKMVTFKFDLDGDNPVEIAEIMVKSDFILESERESFIEQVQLVIQNADQKGKAPQNSQTQVISNSSQQVPEILTSSVPGMPPSLAAQVVHSAGRRFIVSPVPEARLREPLFGTPSANTSFEDTAPAPDPTSASSAPQDQANFASDSNQISVVPPTPGGLAPESSIPPTSQSPYQNPVPSSTATLTVTTTQVTTPTPTTTPQPITGRTSPSPAPTEPPAARRMSLPSPSQTPQIAPGESNGLDQAQASNQQVTQPSATGDSESDVQGKPPGIEDIHALDQKLRSLFKDSSQNSCSQPDGSGETSTTSPPNTISTNTPGFVSGVTPSSLSLNSSGNFAPGAFASIGTPTSYTQTPSTDQSPPRPQISVGVTQPDGHNSTEEKEPQLEKAFQLGRFQVSVASDQDPPTALDPGVVVSSSSATPSSTSSSSSSSSPSSLSSPENTIHKSQTPPPRVSPEPVPPTTTVGRFQVTSSTDIKLGRFSVTPAERKSSSVEGPDTAKEPKLTPSSVSITNHYLSSDNDSEPEDEGLRREMNRLKERHKVEIMALQNKQKKEIEELFTRLGKTPSVVVPPTVAMTPGRRRPAKGKGNRPRSGSATQAASFQQGNKQAVQSGSLSQSASEKTSPSETAPSSNNDVQDNTSPVRKENGQSHDQDQTPTTNLPRKGTFTDDLHQLVDNWARDAMNMMPQVKKGSKKNVHEMPRKYSAPGQLCSTGGHIPATINPTAGRKGSIGVANQQFGYTCPNYNMPQWNQVGTISSTAPPAGLQQGFLIPSGAHQTGNNCGSSNLRTTQAS

Target ORF information:

RefSeq Version	XM_002666846.6
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1a (wnk1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002666846.6

ORF Insert Sequence:

ATGTCAGAAA ATCTCAACGA CAAGATGGTC AAGTTCCTTT CCCCCCCTTC GAAGAACACC 
AACGGCTCCA GCTCAGACAC TCTGGTGGGT GAGCATCTAG GTGTCGATGT CCGTCGTCGC 
CACCACACCA TGGATCGAGA ACTGCTGAAG GCAGAGCACC GTTTCTTTCG CCGCAGCGTC 
ATCAACGACT CCAATGCTAC AGCGCTTGAG CTGCCCAGCA AGAATGCAAT CCTAACCCAC 
TCCACTGACT CCCATGCCCC TGTCTGTGAA CCTCCTGCCC TAGAAACCAA AGCCGCTTCA 
GTGATCACAC AATCTGCACC AGAAAAAAAA GTGGCTGATG TTGTTGAGAT GGTTATTGAG 
TCAACACCAG TGTTGATTAC AAACGCTGCA CTGCATCTTT CTGCAGAAGC ACCTAGTTTG 
GTTTCAGAGG TGAAGTCTGG AGATAGCATT GATGGAAAAC GAGAGGTTGA GAAAGAGGAG 
GATGAAGATA GTAAAGAAAA GGCGGCACGT GCTGAGGCTG AGTTGCGGGA TGCTAAGAAG 
GAGGAGGAAG ACAATGAGGA AATTGAGACT AACGCTGTTG GGACATCACC GGATGGCCGC 
TTCTTGAAGT TTGACATCGA GATAGGACGT GGCTCTTTTA AGACGGTCTA CAAGGGACTG 
GATACGGAAA CTACAGTGGA GGTTGCATGG TGTGAGTTAC AGGATCGCAA GTTGTCCAAG 
TCAGAGCGGC AGCGTTTTAA AGAGGAAGCT GGAATGTTGA AGGGTCTTCA GCATCCCAAC 
ATAGTACGCT TTTATGACTC CTGGGAATCA TCCTTGAAAG GGAGGAAGTG TATTGTCTTG 
GTGACTGAAC TCATGACATC TGGGACACTC AAGACGTATC TAAAACGGTT TAAGGAGATG 
AAGATCAAAG TCTTGCGCAG CTGGTGCCGG CAGATTTTGA AGGGCCTACA CTTCCTTCAT 
ACCAGGTCAC CACCTATCAT CCACCGGGAT CTGAAGTGTG ACAACATCTT CATCACTGGG 
CCTACTGGAT CAGTCAAGAT TGGAGATTTG GGCCTTGCGA CCCTCAAGAG GGCCTCCTTT 
GCTAAGAGCG TCATAGGTAC CCCTGAGTTC ATGGCGCCTG AGATGTATGA AGAGAAGTAT 
GACGAGTCGG TGGATGTGTA TGCCTTTGGG ATGTGTATGC TGGAGATGGC TACTTCTGAG 
TACCCGTACT CCGAGTGTCA GAATGCAGCT CAGATCTATC GCAGAGTCAC CAGCGGGGTG 
AAGCCTGGCA GTTTTGACAA AGTGGCTATT CCAGAGGTGA AGGAGATCAT TGAGGGTTGC 
ATTCGTCAGA ATAAAGATGA GAGGTACTGC ATCAAGGATC TCCTAAGCCA TGCCTTCTTC 
CAGGAGGAGA CTGGAGTTCG TGTAGAACTG GCTGAGGAGG ATGATGGAGA GCTTGTGGCT 
ATAAAGTTGT TGCTGCGCAT TGAGGATGTT AAGAAACTTA AAGGCAAATA CAAAGAGAAT 
GAAGCCATCG AGTTTTCCTT CGACTTGGAT AAAGACAACC CAGATGATGT GGCTCAAGAA 
ATGGTTGAAT CAGGCTACAT TTGTGAGGGT GACCACAAAA CCATTGCAAA GGCCATAAAG 
GACAGAGTGT CCCTGATCTC CCGTAAGAGA GAGCAGAGAA AACTGGTGAG AGAAGAACAG 
GAAAAAAGGA AGATGGAGCA GGAGAATGAC GCCGCCCAGC AAACTAACCC CACAGTCAAG 
TCCCCTGGTT CTGCTCTGGC CTCGGTCATA ATGGAGTCTG AAGAAGCAGA AACAGACCAG 
CACCAGCAGC CTGGAGCCTC TGCTTCTGCT GTAGGGTTTG TGGATCCTCA AAGTTCAGTG 
ACCATCCTCG AGGCTCATCC TGTCCAGCCA AACACATCTT TCAGCACCAC ACAGCCTGAT 
CCGCAGCCAC AGCACACAAT TATACGTCCA CAAAGCATGT CGCAGCACCC AGTCCAGGCC 
CCTCTGCAGC AGCCGAGCAG TACTTTGACC CTGGGCCCCG TCCCCCAGAC TTCTCCACCT 
CAGGTGATTG GTCAAACAGC CTCTCTGCCC TCTACTGTTC AACCTATTGT CCCGCTGCAG 
TACCGTCAGC CACCTCAGGA TGGAATCACA CTACCTCAAT CCCTGCCCAG CGCTCAAGTC 
TTACAGGCAC CACTAAGTGT GTCCCAATCA CAACCAGTAG AGCAGACACA ACCTCAAACA 
TCTCTGGTTC CTGCCTCCAC AGAAAGTGGA CACTCTGATC CGGCATCTGG TCTAAGCGAT 
GGGAATGAGG GGAGACACGA GGGTCGCTCT ATGAAACGAC CCCAACGCCG TTCTGTACGC 
AGCCGCTCGC GTCATGAAAA AACAGCTAAG GTCAAGCTCA ATGTGCTAAA TATTTCTAAT 
ATTGGAGACA GAGTCGCTGA ATGTCAGCTG GAAACCCACA ACAGAAAGAT GGTCACATTC 
AAGTTTGATC TGGATGGGGA TAATCCTGTA GAAATCGCTG AAATTATGGT CAAAAGTGAT 
TTTATTTTGG AGAGTGAACG AGAGTCCTTC ATAGAACAGG TTCAACTGGT CATTCAGAAT 
GCAGATCAGA AAGGAAAAGC TCCCCAAAAT AGCCAAACTC AGGTGATCAG TAATTCGAGC 
CAACAAGTAC CTGAAATTTT AACATCCAGT GTGCCTGGTA TGCCACCTAG CTTAGCAGCA 
CAGGTTGTAC ATTCAGCTGG CAGGCGCTTC ATAGTCAGCC CCGTGCCTGA AGCACGACTT 
CGGGAGCCGT TGTTTGGTAC TCCCTCAGCA AACACATCAT TTGAAGATAC AGCACCAGCA 
CCTGATCCAA CATCAGCCAG CAGTGCTCCG CAGGACCAAG CCAACTTTGC CTCTGATTCA 
AACCAAATTT CTGTGGTACC ACCGACACCT GGAGGTCTTG CCCCAGAGAG CTCCATTCCA 
CCTACTTCTC AATCACCATA CCAGAATCCT GTCCCATCAT CCACTGCAAC TTTGACTGTG 
ACCACCACTC AAGTAACCAC TCCAACACCA ACTACCACTC CGCAACCTAT AACAGGGAGG 
ACGTCTCCTT CACCAGCCCC AACAGAACCA CCAGCAGCAC GTCGTATGTC ACTTCCCAGC 
CCATCCCAGA CACCTCAAAT TGCTCCAGGA GAGAGTAATG GCTTGGATCA AGCACAAGCC 
TCAAATCAGC AAGTAACTCA ACCGAGTGCA ACCGGAGATA GTGAGAGCGA TGTACAGGGG 
AAACCACCTG GAATTGAAGA CATTCATGCT CTAGACCAAA AGCTGCGCTC CCTTTTCAAG 
GACTCATCCC AGAACTCTTG CAGCCAACCT GACGGGTCTG GAGAGACTTC CACAACCTCT 
CCACCCAACA CCATAAGCAC TAACACTCCT GGCTTTGTCT CTGGTGTGAC GCCTTCAAGT 
TTGTCGCTCA ATTCCAGCGG GAACTTTGCA CCTGGTGCTT TCGCCTCCAT TGGTACTCCT 
ACAAGCTACA CCCAGACACC ATCTACCGAC CAGTCCCCTC CAAGGCCACA GATTTCTGTA 
GGTGTAACTC AACCAGATGG ACACAACAGT ACTGAGGAGA AAGAACCACA ACTTGAGAAA 
GCTTTCCAAC TTGGCCGATT TCAGGTGTCT GTGGCCAGTG ATCAAGACCC ACCAACTGCT 
CTAGACCCAG GAGTGGTGGT TTCATCTTCC TCTGCTACCC CATCTTCAAC ATCATCATCA 
TCTTCTTCTT CATCACCATC ATCTCTCTCT AGCCCCGAAA ACACAATTCA TAAATCTCAG 
ACTCCTCCAC CCAGGGTCAG CCCTGAACCC GTACCACCTA CCACTACAGT TGGCCGCTTT 
CAGGTGACCT CCAGCACTGA CATCAAACTT GGTCGCTTCT CAGTGACACC AGCAGAAAGA 
AAGTCTTCCT CAGTTGAGGG ACCAGACACC GCCAAAGAGC CTAAATTGAC CCCTTCCAGT 
GTGTCCATTA CCAATCACTA TCTCAGTAGC GACAACGACT CAGAACCAGA AGATGAGGGC 
TTAAGACGAG AGATGAACAG GCTCAAGGAG AGACATAAGG TTGAGATCAT GGCATTGCAG 
AATAAACAGA AAAAGGAAAT CGAGGAGCTC TTCACTCGTC TGGGCAAAAC ACCATCTGTG 
GTTGTGCCAC CCACTGTGGC CATGACGCCT GGTAGACGGA GACCTGCTAA GGGCAAGGGG 
AACAGGCCAA GAAGTGGTAG TGCCACTCAG GCTGCATCTT TTCAACAAGG GAACAAACAA 
GCTGTGCAGA GTGGTTCACT CAGTCAGTCA GCCAGTGAGA AGACTTCACC TTCAGAAACA 
GCACCAAGCT CAAACAATGA TGTGCAAGAT AATACAAGTC CAGTGAGAAA AGAAAACGGA 
CAGAGCCATG ACCAGGACCA AACACCGACA ACTAATCTGC CACGCAAGGG CACATTCACA 
GATGACCTCC ATCAGCTGGT TGATAACTGG GCCAGAGATG CCATGAACAT GATGCCACAG 
GTCAAGAAAG GATCCAAAAA GAATGTTCAT GAAATGCCAC GAAAATACTC CGCACCAGGT 
CAGCTTTGTT CCACTGGTGG CCACATCCCA GCGACCATCA ACCCCACAGC TGGACGGAAG 
GGTTCAATAG GTGTGGCCAA TCAGCAATTT GGCTACACTT GTCCCAACTA CAACATGCCT 
CAATGGAATC AGGTGGGGAC AATATCATCC ACCGCTCCAC CTGCAGGACT ACAGCAGGGT 
TTCCTTATCC CATCAGGAGC CCACCAAACC GGAAATAACT GCGGATCAAG CAATCTGCGC 
ACCACTCAGG CAAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1a ( XM_002666846.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_002666892.3 Danio rerio WNK lysine deficient protein kinase 1a (wnk1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa29433
Clone ID Related Accession (Same CDS sequence)	XM_002666846.5 , XM_021470687.1
Accession Version	XM_002666846.5 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_002666846.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAAA ATCTCAACGA CAAGATGGTC AAGTTCCTTT CCCCCCCTTC GAAGAACACC 
AACGGCTCCA GCTCAGACAC TTTGGTGGGT GAGCATCTAG GTGTCGATGT CCGTCGTCGC 
CACCACACCA TGGATCGAGA ACTGCTGAAG GCAGAGCACC GTTTCTTTCG CCGCAGCGTC 
ATCAACGACT CTAATGCTAC AGCGCTTGAG CTGCCCAGCA AGAATGCAAT CCTAACCCAC 
TCCACTGACT CCCATGCCCC TGTCTGTGAA CCTCCTGCCC TAGAAACCAA AGCCGCTTCA 
GTGATCACAC AATCTGCACC AGAAAAAAAA GTGGCTGATG TTGTTGAGAT GGTTATTGAG 
TCAACACCAG TGTTGATTAC AAACGCTGCA CTGCATCTTT CTGCAGAAGC ACCTAGTTTG 
GTTTCAGAGG TAAAGTCTGG AGATGGCATT GATGGAAAAC GAGAGGCTGA GAAAGAGGAG 
GATGAAGATA GTAAAGAAAA GGCGGCACGT GCTGAGGCTG AGTTGCGGGA TGCTAAGAAG 
GAGGAGGAAG ACAATGAGGA AATTGAGACT AATGCTGTTG GGACGTCACC GGATGGCCGC 
TTCTTGAAGT TTGACATCGA GATAGGACGT GGCTCTTTTA AGACGGTCTA CAAGGGACTG 
GATACGGAAA CTACAGTGGA GGTTGCATGG TGTGAGTTAC AGGATCGCAA GTTGTCCAAG 
TCAGAGCGGC AGCGTTTTAA AGAGGAAGCT GGAATGTTGA AGGGTCTTCA GCATCCCAAC 
ATAGTACGCT TTTATGACTC CTGGGAATCA TCCTTGAAAG GGAGGAAGTG TATTGTCTTG 
GTGACTGAAC TCATGACATC TGGGACACTC AAGACGTATC TAAAACGGTT TAAGGAGATG 
AAGATCAAAG TCTTGCGCAG CTGGTGCCGG CAGATTTTGA AGGGCCTACA CTTCCTTCAT 
ACCAGGTCAC CACCTATCAT CCACCGGGAT CTGAAGTGTG ACAACATCTT CATCACTGGG 
CCTACTGGAT CAGTCAAGAT TGGAGATTTG GGCCTTGCGA CCCTCAAGAG GGCCTCCTTT 
GCTAAGAGCG TCATAGGTAC CCCTGAGTTC ATGGCGCCTG AGATGTATGA AGAGAAGTAT 
GACGAGTCGG TGGATGTGTA TGCCTTTGGG ATGTGTATGC TGGAGATGGC TACTTCTGAG 
TACCCGTACT CCGAGTGTCA GAATGCAGCT CAGATCTATC GCAGAGTCAC CAGCGGGGTG 
AAGCCTGGCA GTTTTGACAA AGTGGCAATT CCAGAGGTGA AGGAGATCAT TGAGGGTTGC 
ATTCGTCAGA ATAAAGATGA GAGGTACTGC ATCAAGGATC TCCTAAGCCA TGCCTTCTTC 
CAGGAGGAGA CTGGAGTTCG TGTAGAACTG GCTGAGGAGG ATGATGGAGA GCTTGTGGCT 
ATAAAATTGT TGCTGCGCAT TGAGGATGTT AAGAAACTTA AAGGCAAATA CAAAGAGAAT 
GAAGCCATCG AGTTTTCCTT CGACTTGGAT AAAGACGTCC CAGATGATGT GGCTCAAGAA 
ATGGTTGAAT CAGGCTACAT TTGTGAGGGT GACCACAAAA CCATTGCAAA GGCCATAAAG 
GACAGAGTGT CCCTGATCTC CCGTAAGAGA GAGCAGAGAA AACTGGTGAG AGAAGAACAG 
GAAAAAAGGA AGATGGAGCA GGAGAATGAC GCCGCCCAGC AAACTAACCC CACAGTCAAG 
TCCCCTGGTT CTGCTCTGGC CTCAGTCATA ATGGAGTCTG AAGAAGCAGA AACAGACCAG 
CACCAGCAGC CTGGAGCCTC TGCTTCTGCT GTAGGGTTTG TGGATCCTCA AAGTTCAGTG 
ACCATCCTCG AGGCTCATCC TGTCCAGCCA AACACATCTT TCAGCACCAC ACAGCCTGAT 
CCGCAGCCAC AGCACACAAT TATACGTCCA CAAAGCATGT CGCAGCACCC AGTCCAGGCC 
CCTCTGCAGC AGCCGAGCAG TACTTTGACC CTGGGCCCCG TCCCCCAGAC TTCTCCACCT 
CAGGTGATCG GTCAAACAGC CTCTCTGCCC TCTACTGTTC AATCTATTGT CCCGCTGCAG 
TACCGTCAGC CACCTCAGGA TGGAATCACA CTACCTCAAT CCCTGCCCAG CGCTCAAGTC 
TTACAGGCAC CACTAAGTGT GTCCCAATCA CAACCAGTAG AGCAGACACA ACCTCAAACA 
TCTCTGGTTC CTGCCTCCAC AGAAAGTGGA CACTCTGATC CGGCATCTGG TCTAAGCGAT 
GGGAATGAGG GGAGACACGA GGGTCGCTCT ATGAAACGAC CCCAACGCCG TTCTGTACGC 
AGCCGCTCGC GTCATGAAAA AACAGCTAAG GTCAAGCTCA ATGTGCTAAA TATTTCTAAT 
ATTGGAGACA GAGTCGCTGA ATGTCAGCTG GAAACCCACA ACAGAAAGAT GGTCACATTC 
AAGTTTGATC TGGATGGGGA TAATCCTGTA GAAATCGCTG AAATTATGGT CAAAAGTGAT 
TTTATTTTGG AGAGTGAACG AGAGTCCTTC ATAGAACAGG TTCAGCTGGT CATTCAGAAT 
GCAGATCAGA AAGGAAAAGC TCCCCAAAAT AGCCAAACTC AGGTGATCAG TAATTCGAGC 
CAACAAGTAC CTGAAATTTT AACATCCAGT GTGCCTGGTA TGCCACCTAG CTTAGCAGCA 
CAGGTTGTAC ATTCAGCAGG CAGGCGCTTC ATAGTCAGCC CCGTGCCTGA AGCACGACTT 
CGGGAGCCGT TGTTTGGTAC TCCCTCAGCA AACACATCAT TTGAAGATAC GGCACCAGCA 
CCTGATCCAA CATCAGCCAG CAGTGCTCCG CAGGACCAAG CCAACTTTGC CTCTGATTCA 
AACCAAATTT CTGTGGTACC ACCGACACCT GGAGATCTTG CCCCAGAGAG CTCCATTCCA 
CCTACTTCTC AATCACCATA CCAGAATCCT GTCCCATCAT CCACTGCAAC TTTGACTGTG 
ACCACCACTC AAGTAAGCAC TCCAACACCA ACTACCACTC CGCAACCTAT AACAGGGAGG 
ACGTCTCCTT CACCAGCCCC AACAGAACCA CCAGCAGCAC GTCGTATGTC ACTTCCCAGC 
CCATCCCAGA CACCTCAAAT TGCTCCAGGA GAGAGTAATG GCTTGGATCA AGCACAAGCC 
TCAAATCAGC AAGTAACTCA ACCGAGTGCA ACCGGAGATA GTGAGAGCGA CGTACAGGGG 
AAACCACCTG GAATTGAAGA CATTCATGCT CTAGACCAAA AGCTGCGCTC CCTTTTCAAG 
GACTCATCCC AGAACTCTTG CAGCCAACCT GACGGGTCTG GAGAGACTTC CACAACCTCT 
CCACCCAACA CCATAAGCAC TAACACTCCT GGCTTTGTCT CTGGTGTGAC GCCTTCTAGT 
TTGTCGCTCA ATTCCAGCGG GAACTTTGCA CCTGGTGCTT TCGCCTCCAT TGGTACTCCT 
ACAAGCTACA CCCAGACACC ATCTACCGAC CAGTCCCCTC CAAGGCCACA GATTGCTGTT 
GGTGTAACTC AACCAGATGG ACACAACAGT ACTGAGGAGA AAGAGCCACA ACTTGAGAAA 
GCTTTCCAAC TTGGCCGATT TCAGGTGTCT GTGGCCAGTG ATCAAGACCC ACCAACTGCT 
CTAGACCCAG GAGTGGTGGT TTCATCTTCC TCTGCTACCC CATCTTCAAC ATCATCATCA 
TCATCATCTT CTTCATCACC ATCATCTCTC TCTAGCCCCG AAAACACAAT TCATAAATCT 
CAGACTCCTC CACCCAGAGT CAGCCCTGAA CCCGTACCAC CTACCACTAC AGTTGGCCGC 
TTTCAGGTGA CTTGCAGCAC TGACATCAAA CTTGGTCGCT TCTCAGTGAC ACCAGCAGAA 
AGAAAGTCTT CCTCAGTTGA GGGACCAGAC ACCGCCAAAG AGCCTAAATT GACCCCTTCC 
AGTGTGTCCA TTACCAATCA TTATCTCAGT AGCGACAACG ACTCAGAACC AGAAGATGAG 
GGCTTAAGAC GAGAGATGAA CAGGCTCAAG GAGAGACATA AGGTTGAGAT CATGGCATTG 
CAGAATAAAC AGAAAAAGGA AATTGAGGAG CTCTTCACTC GTCTGGGCAA AACACCATCT 
GTGGTTGTGC CACCCACTGT GGCCATGACG CCTGGTAGAC GGAGACCTGC TAAGGGCAAG 
GGGAACAGGC CAAGAAGTGG TAGTGCCACT CAGGCTGCAT CTTTTCAACA AGGGAACAAA 
CAAGCTGTGC AGAGTGGTTC ACTCAGTCAG TCAGCCAGTG AGAAGACTTC ACCTTCAGAA 
ACAGCACCAA GCTCAAACAA TGATGTGCAA GATAATACAA GTCCAGTGAG AAAAGAAAAC 
GGACAGAGCC ATGACCAGGA CCAAACACCA ACAACTAATC TGCCACGCAA GGGCACATTC 
ACAGATGACC TCCATCAGCT GGTTGATAAC TGGGCCAGAG ATGCCATGAA CATGATGCCA 
CAGGTCAAGA AAGGATCCAA AAAGAATGTT CATGAAATGC CACGTAAATA CTCCGCACCA 
GGTCAGCTTT GTTCCACTGG TGGCCACATC CCAGCGACCA TCAACCCCAC AGCTGGACGG 
AAGGGTTCAA TAGGTGTGGC CAATCAGCAA TTTGGCTACA CTTGTCCCAA CTACAACATG 
CCTCAATGGA ATCAGGTGGG GACAATATCA TCCACCGCTC CACCTGCAGG ACTACAGCAG 
GGTTTCCTTA TCCCATCAGG AGCCCACCAA ACCGGAAATA ACTGCGGATC AAGCAATCTG 
CGCACCACTC AGGCAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002666892.3
CDS	1196..6016
Translation	MSENLNDKMVKFLSPPSKNTNGSSSDTLVGEHLGVDVRRRHHTMDRELLKAEHRFFRRSVINDSNATALELPSKNAILTHSTDSHAPVCEPPALETKAASVITQSAPEKKVADVVEMVIESTPVLITNAALHLSAEAPSLVSEVKSGDGIDGKREAEKEEDEDSKEKAARAEAELRDAKKEEEDNEEIETNAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSKSERQRFKEEAGMLKGLQHPNIVRFYDSWESSLKGRKCIVLVTELMTSGTLKTYLKRFKEMKIKVLRSWCRQILKGLHFLHTRSPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYCIKDLLSHAFFQEETGVRVELAEEDDGELVAIKLLLRIEDVKKLKGKYKENEAIEFSFDLDKDVPDDVAQEMVESGYICEGDHKTIAKAIKDRVSLISRKREQRKLVREEQEKRKMEQENDAAQQTNPTVKSPGSALASVIMESEEAETDQHQQPGASASAVGFVDPQSSVTILEAHPVQPNTSFSTTQPDPQPQHTIIRPQSMSQHPVQAPLQQPSSTLTLGPVPQTSPPQVIGQTASLPSTVQSIVPLQYRQPPQDGITLPQSLPSAQVLQAPLSVSQSQPVEQTQPQTSLVPASTESGHSDPASGLSDGNEGRHEGRSMKRPQRRSVRSRSRHEKTAKVKLNVLNISNIGDRVAECQLETHNRKMVTFKFDLDGDNPVEIAEIMVKSDFILESERESFIEQVQLVIQNADQKGKAPQNSQTQVISNSSQQVPEILTSSVPGMPPSLAAQVVHSAGRRFIVSPVPEARLREPLFGTPSANTSFEDTAPAPDPTSASSAPQDQANFASDSNQISVVPPTPGDLAPESSIPPTSQSPYQNPVPSSTATLTVTTTQVSTPTPTTTPQPITGRTSPSPAPTEPPAARRMSLPSPSQTPQIAPGESNGLDQAQASNQQVTQPSATGDSESDVQGKPPGIEDIHALDQKLRSLFKDSSQNSCSQPDGSGETSTTSPPNTISTNTPGFVSGVTPSSLSLNSSGNFAPGAFASIGTPTSYTQTPSTDQSPPRPQIAVGVTQPDGHNSTEEKEPQLEKAFQLGRFQVSVASDQDPPTALDPGVVVSSSSATPSSTSSSSSSSSSPSSLSSPENTIHKSQTPPPRVSPEPVPPTTTVGRFQVTCSTDIKLGRFSVTPAERKSSSVEGPDTAKEPKLTPSSVSITNHYLSSDNDSEPEDEGLRREMNRLKERHKVEIMALQNKQKKEIEELFTRLGKTPSVVVPPTVAMTPGRRRPAKGKGNRPRSGSATQAASFQQGNKQAVQSGSLSQSASEKTSPSETAPSSNNDVQDNTSPVRKENGQSHDQDQTPTTNLPRKGTFTDDLHQLVDNWARDAMNMMPQVKKGSKKNVHEMPRKYSAPGQLCSTGGHIPATINPTAGRKGSIGVANQQFGYTCPNYNMPQWNQVGTISSTAPPAGLQQGFLIPSGAHQTGNNCGSSNLRTTQAS

Target ORF information:

RefSeq Version	XM_002666846.5
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1a (wnk1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002666846.5

ORF Insert Sequence:

ATGTCAGAAA ATCTCAACGA CAAGATGGTC AAGTTCCTTT CCCCCCCTTC GAAGAACACC 
AACGGCTCCA GCTCAGACAC TTTGGTGGGT GAGCATCTAG GTGTCGATGT CCGTCGTCGC 
CACCACACCA TGGATCGAGA ACTGCTGAAG GCAGAGCACC GTTTCTTTCG CCGCAGCGTC 
ATCAACGACT CTAATGCTAC AGCGCTTGAG CTGCCCAGCA AGAATGCAAT CCTAACCCAC 
TCCACTGACT CCCATGCCCC TGTCTGTGAA CCTCCTGCCC TAGAAACCAA AGCCGCTTCA 
GTGATCACAC AATCTGCACC AGAAAAAAAA GTGGCTGATG TTGTTGAGAT GGTTATTGAG 
TCAACACCAG TGTTGATTAC AAACGCTGCA CTGCATCTTT CTGCAGAAGC ACCTAGTTTG 
GTTTCAGAGG TAAAGTCTGG AGATGGCATT GATGGAAAAC GAGAGGCTGA GAAAGAGGAG 
GATGAAGATA GTAAAGAAAA GGCGGCACGT GCTGAGGCTG AGTTGCGGGA TGCTAAGAAG 
GAGGAGGAAG ACAATGAGGA AATTGAGACT AATGCTGTTG GGACGTCACC GGATGGCCGC 
TTCTTGAAGT TTGACATCGA GATAGGACGT GGCTCTTTTA AGACGGTCTA CAAGGGACTG 
GATACGGAAA CTACAGTGGA GGTTGCATGG TGTGAGTTAC AGGATCGCAA GTTGTCCAAG 
TCAGAGCGGC AGCGTTTTAA AGAGGAAGCT GGAATGTTGA AGGGTCTTCA GCATCCCAAC 
ATAGTACGCT TTTATGACTC CTGGGAATCA TCCTTGAAAG GGAGGAAGTG TATTGTCTTG 
GTGACTGAAC TCATGACATC TGGGACACTC AAGACGTATC TAAAACGGTT TAAGGAGATG 
AAGATCAAAG TCTTGCGCAG CTGGTGCCGG CAGATTTTGA AGGGCCTACA CTTCCTTCAT 
ACCAGGTCAC CACCTATCAT CCACCGGGAT CTGAAGTGTG ACAACATCTT CATCACTGGG 
CCTACTGGAT CAGTCAAGAT TGGAGATTTG GGCCTTGCGA CCCTCAAGAG GGCCTCCTTT 
GCTAAGAGCG TCATAGGTAC CCCTGAGTTC ATGGCGCCTG AGATGTATGA AGAGAAGTAT 
GACGAGTCGG TGGATGTGTA TGCCTTTGGG ATGTGTATGC TGGAGATGGC TACTTCTGAG 
TACCCGTACT CCGAGTGTCA GAATGCAGCT CAGATCTATC GCAGAGTCAC CAGCGGGGTG 
AAGCCTGGCA GTTTTGACAA AGTGGCAATT CCAGAGGTGA AGGAGATCAT TGAGGGTTGC 
ATTCGTCAGA ATAAAGATGA GAGGTACTGC ATCAAGGATC TCCTAAGCCA TGCCTTCTTC 
CAGGAGGAGA CTGGAGTTCG TGTAGAACTG GCTGAGGAGG ATGATGGAGA GCTTGTGGCT 
ATAAAATTGT TGCTGCGCAT TGAGGATGTT AAGAAACTTA AAGGCAAATA CAAAGAGAAT 
GAAGCCATCG AGTTTTCCTT CGACTTGGAT AAAGACGTCC CAGATGATGT GGCTCAAGAA 
ATGGTTGAAT CAGGCTACAT TTGTGAGGGT GACCACAAAA CCATTGCAAA GGCCATAAAG 
GACAGAGTGT CCCTGATCTC CCGTAAGAGA GAGCAGAGAA AACTGGTGAG AGAAGAACAG 
GAAAAAAGGA AGATGGAGCA GGAGAATGAC GCCGCCCAGC AAACTAACCC CACAGTCAAG 
TCCCCTGGTT CTGCTCTGGC CTCAGTCATA ATGGAGTCTG AAGAAGCAGA AACAGACCAG 
CACCAGCAGC CTGGAGCCTC TGCTTCTGCT GTAGGGTTTG TGGATCCTCA AAGTTCAGTG 
ACCATCCTCG AGGCTCATCC TGTCCAGCCA AACACATCTT TCAGCACCAC ACAGCCTGAT 
CCGCAGCCAC AGCACACAAT TATACGTCCA CAAAGCATGT CGCAGCACCC AGTCCAGGCC 
CCTCTGCAGC AGCCGAGCAG TACTTTGACC CTGGGCCCCG TCCCCCAGAC TTCTCCACCT 
CAGGTGATCG GTCAAACAGC CTCTCTGCCC TCTACTGTTC AATCTATTGT CCCGCTGCAG 
TACCGTCAGC CACCTCAGGA TGGAATCACA CTACCTCAAT CCCTGCCCAG CGCTCAAGTC 
TTACAGGCAC CACTAAGTGT GTCCCAATCA CAACCAGTAG AGCAGACACA ACCTCAAACA 
TCTCTGGTTC CTGCCTCCAC AGAAAGTGGA CACTCTGATC CGGCATCTGG TCTAAGCGAT 
GGGAATGAGG GGAGACACGA GGGTCGCTCT ATGAAACGAC CCCAACGCCG TTCTGTACGC 
AGCCGCTCGC GTCATGAAAA AACAGCTAAG GTCAAGCTCA ATGTGCTAAA TATTTCTAAT 
ATTGGAGACA GAGTCGCTGA ATGTCAGCTG GAAACCCACA ACAGAAAGAT GGTCACATTC 
AAGTTTGATC TGGATGGGGA TAATCCTGTA GAAATCGCTG AAATTATGGT CAAAAGTGAT 
TTTATTTTGG AGAGTGAACG AGAGTCCTTC ATAGAACAGG TTCAGCTGGT CATTCAGAAT 
GCAGATCAGA AAGGAAAAGC TCCCCAAAAT AGCCAAACTC AGGTGATCAG TAATTCGAGC 
CAACAAGTAC CTGAAATTTT AACATCCAGT GTGCCTGGTA TGCCACCTAG CTTAGCAGCA 
CAGGTTGTAC ATTCAGCAGG CAGGCGCTTC ATAGTCAGCC CCGTGCCTGA AGCACGACTT 
CGGGAGCCGT TGTTTGGTAC TCCCTCAGCA AACACATCAT TTGAAGATAC GGCACCAGCA 
CCTGATCCAA CATCAGCCAG CAGTGCTCCG CAGGACCAAG CCAACTTTGC CTCTGATTCA 
AACCAAATTT CTGTGGTACC ACCGACACCT GGAGATCTTG CCCCAGAGAG CTCCATTCCA 
CCTACTTCTC AATCACCATA CCAGAATCCT GTCCCATCAT CCACTGCAAC TTTGACTGTG 
ACCACCACTC AAGTAAGCAC TCCAACACCA ACTACCACTC CGCAACCTAT AACAGGGAGG 
ACGTCTCCTT CACCAGCCCC AACAGAACCA CCAGCAGCAC GTCGTATGTC ACTTCCCAGC 
CCATCCCAGA CACCTCAAAT TGCTCCAGGA GAGAGTAATG GCTTGGATCA AGCACAAGCC 
TCAAATCAGC AAGTAACTCA ACCGAGTGCA ACCGGAGATA GTGAGAGCGA CGTACAGGGG 
AAACCACCTG GAATTGAAGA CATTCATGCT CTAGACCAAA AGCTGCGCTC CCTTTTCAAG 
GACTCATCCC AGAACTCTTG CAGCCAACCT GACGGGTCTG GAGAGACTTC CACAACCTCT 
CCACCCAACA CCATAAGCAC TAACACTCCT GGCTTTGTCT CTGGTGTGAC GCCTTCTAGT 
TTGTCGCTCA ATTCCAGCGG GAACTTTGCA CCTGGTGCTT TCGCCTCCAT TGGTACTCCT 
ACAAGCTACA CCCAGACACC ATCTACCGAC CAGTCCCCTC CAAGGCCACA GATTGCTGTT 
GGTGTAACTC AACCAGATGG ACACAACAGT ACTGAGGAGA AAGAGCCACA ACTTGAGAAA 
GCTTTCCAAC TTGGCCGATT TCAGGTGTCT GTGGCCAGTG ATCAAGACCC ACCAACTGCT 
CTAGACCCAG GAGTGGTGGT TTCATCTTCC TCTGCTACCC CATCTTCAAC ATCATCATCA 
TCATCATCTT CTTCATCACC ATCATCTCTC TCTAGCCCCG AAAACACAAT TCATAAATCT 
CAGACTCCTC CACCCAGAGT CAGCCCTGAA CCCGTACCAC CTACCACTAC AGTTGGCCGC 
TTTCAGGTGA CTTGCAGCAC TGACATCAAA CTTGGTCGCT TCTCAGTGAC ACCAGCAGAA 
AGAAAGTCTT CCTCAGTTGA GGGACCAGAC ACCGCCAAAG AGCCTAAATT GACCCCTTCC 
AGTGTGTCCA TTACCAATCA TTATCTCAGT AGCGACAACG ACTCAGAACC AGAAGATGAG 
GGCTTAAGAC GAGAGATGAA CAGGCTCAAG GAGAGACATA AGGTTGAGAT CATGGCATTG 
CAGAATAAAC AGAAAAAGGA AATTGAGGAG CTCTTCACTC GTCTGGGCAA AACACCATCT 
GTGGTTGTGC CACCCACTGT GGCCATGACG CCTGGTAGAC GGAGACCTGC TAAGGGCAAG 
GGGAACAGGC CAAGAAGTGG TAGTGCCACT CAGGCTGCAT CTTTTCAACA AGGGAACAAA 
CAAGCTGTGC AGAGTGGTTC ACTCAGTCAG TCAGCCAGTG AGAAGACTTC ACCTTCAGAA 
ACAGCACCAA GCTCAAACAA TGATGTGCAA GATAATACAA GTCCAGTGAG AAAAGAAAAC 
GGACAGAGCC ATGACCAGGA CCAAACACCA ACAACTAATC TGCCACGCAA GGGCACATTC 
ACAGATGACC TCCATCAGCT GGTTGATAAC TGGGCCAGAG ATGCCATGAA CATGATGCCA 
CAGGTCAAGA AAGGATCCAA AAAGAATGTT CATGAAATGC CACGTAAATA CTCCGCACCA 
GGTCAGCTTT GTTCCACTGG TGGCCACATC CCAGCGACCA TCAACCCCAC AGCTGGACGG 
AAGGGTTCAA TAGGTGTGGC CAATCAGCAA TTTGGCTACA CTTGTCCCAA CTACAACATG 
CCTCAATGGA ATCAGGTGGG GACAATATCA TCCACCGCTC CACCTGCAGG ACTACAGCAG 
GGTTTCCTTA TCCCATCAGG AGCCCACCAA ACCGGAAATA ACTGCGGATC AAGCAATCTG 
CGCACCACTC AGGCAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1a ( XM_021470687.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326362.1 Danio rerio WNK lysine deficient protein kinase 1a (wnk1a), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa29433
Clone ID Related Accession (Same CDS sequence)	XM_002666846.5 , XM_021470687.1
Accession Version	XM_021470687.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4821bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAAA ATCTCAACGA CAAGATGGTC AAGTTCCTTT CCCCCCCTTC GAAGAACACC 
AACGGCTCCA GCTCAGACAC TTTGGTGGGT GAGCATCTAG GTGTCGATGT CCGTCGTCGC 
CACCACACCA TGGATCGAGA ACTGCTGAAG GCAGAGCACC GTTTCTTTCG CCGCAGCGTC 
ATCAACGACT CTAATGCTAC AGCGCTTGAG CTGCCCAGCA AGAATGCAAT CCTAACCCAC 
TCCACTGACT CCCATGCCCC TGTCTGTGAA CCTCCTGCCC TAGAAACCAA AGCCGCTTCA 
GTGATCACAC AATCTGCACC AGAAAAAAAA GTGGCTGATG TTGTTGAGAT GGTTATTGAG 
TCAACACCAG TGTTGATTAC AAACGCTGCA CTGCATCTTT CTGCAGAAGC ACCTAGTTTG 
GTTTCAGAGG TAAAGTCTGG AGATGGCATT GATGGAAAAC GAGAGGCTGA GAAAGAGGAG 
GATGAAGATA GTAAAGAAAA GGCGGCACGT GCTGAGGCTG AGTTGCGGGA TGCTAAGAAG 
GAGGAGGAAG ACAATGAGGA AATTGAGACT AATGCTGTTG GGACGTCACC GGATGGCCGC 
TTCTTGAAGT TTGACATCGA GATAGGACGT GGCTCTTTTA AGACGGTCTA CAAGGGACTG 
GATACGGAAA CTACAGTGGA GGTTGCATGG TGTGAGTTAC AGGATCGCAA GTTGTCCAAG 
TCAGAGCGGC AGCGTTTTAA AGAGGAAGCT GGAATGTTGA AGGGTCTTCA GCATCCCAAC 
ATAGTACGCT TTTATGACTC CTGGGAATCA TCCTTGAAAG GGAGGAAGTG TATTGTCTTG 
GTGACTGAAC TCATGACATC TGGGACACTC AAGACGTATC TAAAACGGTT TAAGGAGATG 
AAGATCAAAG TCTTGCGCAG CTGGTGCCGG CAGATTTTGA AGGGCCTACA CTTCCTTCAT 
ACCAGGTCAC CACCTATCAT CCACCGGGAT CTGAAGTGTG ACAACATCTT CATCACTGGG 
CCTACTGGAT CAGTCAAGAT TGGAGATTTG GGCCTTGCGA CCCTCAAGAG GGCCTCCTTT 
GCTAAGAGCG TCATAGGTAC CCCTGAGTTC ATGGCGCCTG AGATGTATGA AGAGAAGTAT 
GACGAGTCGG TGGATGTGTA TGCCTTTGGG ATGTGTATGC TGGAGATGGC TACTTCTGAG 
TACCCGTACT CCGAGTGTCA GAATGCAGCT CAGATCTATC GCAGAGTCAC CAGCGGGGTG 
AAGCCTGGCA GTTTTGACAA AGTGGCAATT CCAGAGGTGA AGGAGATCAT TGAGGGTTGC 
ATTCGTCAGA ATAAAGATGA GAGGTACTGC ATCAAGGATC TCCTAAGCCA TGCCTTCTTC 
CAGGAGGAGA CTGGAGTTCG TGTAGAACTG GCTGAGGAGG ATGATGGAGA GCTTGTGGCT 
ATAAAATTGT TGCTGCGCAT TGAGGATGTT AAGAAACTTA AAGGCAAATA CAAAGAGAAT 
GAAGCCATCG AGTTTTCCTT CGACTTGGAT AAAGACGTCC CAGATGATGT GGCTCAAGAA 
ATGGTTGAAT CAGGCTACAT TTGTGAGGGT GACCACAAAA CCATTGCAAA GGCCATAAAG 
GACAGAGTGT CCCTGATCTC CCGTAAGAGA GAGCAGAGAA AACTGGTGAG AGAAGAACAG 
GAAAAAAGGA AGATGGAGCA GGAGAATGAC GCCGCCCAGC AAACTAACCC CACAGTCAAG 
TCCCCTGGTT CTGCTCTGGC CTCAGTCATA ATGGAGTCTG AAGAAGCAGA AACAGACCAG 
CACCAGCAGC CTGGAGCCTC TGCTTCTGCT GTAGGGTTTG TGGATCCTCA AAGTTCAGTG 
ACCATCCTCG AGGCTCATCC TGTCCAGCCA AACACATCTT TCAGCACCAC ACAGCCTGAT 
CCGCAGCCAC AGCACACAAT TATACGTCCA CAAAGCATGT CGCAGCACCC AGTCCAGGCC 
CCTCTGCAGC AGCCGAGCAG TACTTTGACC CTGGGCCCCG TCCCCCAGAC TTCTCCACCT 
CAGGTGATCG GTCAAACAGC CTCTCTGCCC TCTACTGTTC AATCTATTGT CCCGCTGCAG 
TACCGTCAGC CACCTCAGGA TGGAATCACA CTACCTCAAT CCCTGCCCAG CGCTCAAGTC 
TTACAGGCAC CACTAAGTGT GTCCCAATCA CAACCAGTAG AGCAGACACA ACCTCAAACA 
TCTCTGGTTC CTGCCTCCAC AGAAAGTGGA CACTCTGATC CGGCATCTGG TCTAAGCGAT 
GGGAATGAGG GGAGACACGA GGGTCGCTCT ATGAAACGAC CCCAACGCCG TTCTGTACGC 
AGCCGCTCGC GTCATGAAAA AACAGCTAAG GTCAAGCTCA ATGTGCTAAA TATTTCTAAT 
ATTGGAGACA GAGTCGCTGA ATGTCAGCTG GAAACCCACA ACAGAAAGAT GGTCACATTC 
AAGTTTGATC TGGATGGGGA TAATCCTGTA GAAATCGCTG AAATTATGGT CAAAAGTGAT 
TTTATTTTGG AGAGTGAACG AGAGTCCTTC ATAGAACAGG TTCAGCTGGT CATTCAGAAT 
GCAGATCAGA AAGGAAAAGC TCCCCAAAAT AGCCAAACTC AGGTGATCAG TAATTCGAGC 
CAACAAGTAC CTGAAATTTT AACATCCAGT GTGCCTGGTA TGCCACCTAG CTTAGCAGCA 
CAGGTTGTAC ATTCAGCAGG CAGGCGCTTC ATAGTCAGCC CCGTGCCTGA AGCACGACTT 
CGGGAGCCGT TGTTTGGTAC TCCCTCAGCA AACACATCAT TTGAAGATAC GGCACCAGCA 
CCTGATCCAA CATCAGCCAG CAGTGCTCCG CAGGACCAAG CCAACTTTGC CTCTGATTCA 
AACCAAATTT CTGTGGTACC ACCGACACCT GGAGATCTTG CCCCAGAGAG CTCCATTCCA 
CCTACTTCTC AATCACCATA CCAGAATCCT GTCCCATCAT CCACTGCAAC TTTGACTGTG 
ACCACCACTC AAGTAAGCAC TCCAACACCA ACTACCACTC CGCAACCTAT AACAGGGAGG 
ACGTCTCCTT CACCAGCCCC AACAGAACCA CCAGCAGCAC GTCGTATGTC ACTTCCCAGC 
CCATCCCAGA CACCTCAAAT TGCTCCAGGA GAGAGTAATG GCTTGGATCA AGCACAAGCC 
TCAAATCAGC AAGTAACTCA ACCGAGTGCA ACCGGAGATA GTGAGAGCGA CGTACAGGGG 
AAACCACCTG GAATTGAAGA CATTCATGCT CTAGACCAAA AGCTGCGCTC CCTTTTCAAG 
GACTCATCCC AGAACTCTTG CAGCCAACCT GACGGGTCTG GAGAGACTTC CACAACCTCT 
CCACCCAACA CCATAAGCAC TAACACTCCT GGCTTTGTCT CTGGTGTGAC GCCTTCTAGT 
TTGTCGCTCA ATTCCAGCGG GAACTTTGCA CCTGGTGCTT TCGCCTCCAT TGGTACTCCT 
ACAAGCTACA CCCAGACACC ATCTACCGAC CAGTCCCCTC CAAGGCCACA GATTGCTGTT 
GGTGTAACTC AACCAGATGG ACACAACAGT ACTGAGGAGA AAGAGCCACA ACTTGAGAAA 
GCTTTCCAAC TTGGCCGATT TCAGGTGTCT GTGGCCAGTG ATCAAGACCC ACCAACTGCT 
CTAGACCCAG GAGTGGTGGT TTCATCTTCC TCTGCTACCC CATCTTCAAC ATCATCATCA 
TCATCATCTT CTTCATCACC ATCATCTCTC TCTAGCCCCG AAAACACAAT TCATAAATCT 
CAGACTCCTC CACCCAGAGT CAGCCCTGAA CCCGTACCAC CTACCACTAC AGTTGGCCGC 
TTTCAGGTGA CTTGCAGCAC TGACATCAAA CTTGGTCGCT TCTCAGTGAC ACCAGCAGAA 
AGAAAGTCTT CCTCAGTTGA GGGACCAGAC ACCGCCAAAG AGCCTAAATT GACCCCTTCC 
AGTGTGTCCA TTACCAATCA TTATCTCAGT AGCGACAACG ACTCAGAACC AGAAGATGAG 
GGCTTAAGAC GAGAGATGAA CAGGCTCAAG GAGAGACATA AGGTTGAGAT CATGGCATTG 
CAGAATAAAC AGAAAAAGGA AATTGAGGAG CTCTTCACTC GTCTGGGCAA AACACCATCT 
GTGGTTGTGC CACCCACTGT GGCCATGACG CCTGGTAGAC GGAGACCTGC TAAGGGCAAG 
GGGAACAGGC CAAGAAGTGG TAGTGCCACT CAGGCTGCAT CTTTTCAACA AGGGAACAAA 
CAAGCTGTGC AGAGTGGTTC ACTCAGTCAG TCAGCCAGTG AGAAGACTTC ACCTTCAGAA 
ACAGCACCAA GCTCAAACAA TGATGTGCAA GATAATACAA GTCCAGTGAG AAAAGAAAAC 
GGACAGAGCC ATGACCAGGA CCAAACACCA ACAACTAATC TGCCACGCAA GGGCACATTC 
ACAGATGACC TCCATCAGCT GGTTGATAAC TGGGCCAGAG ATGCCATGAA CATGATGCCA 
CAGGTCAAGA AAGGATCCAA AAAGAATGTT CATGAAATGC CACGTAAATA CTCCGCACCA 
GGTCAGCTTT GTTCCACTGG TGGCCACATC CCAGCGACCA TCAACCCCAC AGCTGGACGG 
AAGGGTTCAA TAGGTGTGGC CAATCAGCAA TTTGGCTACA CTTGTCCCAA CTACAACATG 
CCTCAATGGA ATCAGGTGGG GACAATATCA TCCACCGCTC CACCTGCAGG ACTACAGCAG 
GGTTTCCTTA TCCCATCAGG AGCCCACCAA ACCGGAAATA ACTGCGGATC AAGCAATCTG 
CGCACCACTC AGGCAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326362.1
CDS	1193..6013
Translation	MSENLNDKMVKFLSPPSKNTNGSSSDTLVGEHLGVDVRRRHHTMDRELLKAEHRFFRRSVINDSNATALELPSKNAILTHSTDSHAPVCEPPALETKAASVITQSAPEKKVADVVEMVIESTPVLITNAALHLSAEAPSLVSEVKSGDGIDGKREAEKEEDEDSKEKAARAEAELRDAKKEEEDNEEIETNAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSKSERQRFKEEAGMLKGLQHPNIVRFYDSWESSLKGRKCIVLVTELMTSGTLKTYLKRFKEMKIKVLRSWCRQILKGLHFLHTRSPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYCIKDLLSHAFFQEETGVRVELAEEDDGELVAIKLLLRIEDVKKLKGKYKENEAIEFSFDLDKDVPDDVAQEMVESGYICEGDHKTIAKAIKDRVSLISRKREQRKLVREEQEKRKMEQENDAAQQTNPTVKSPGSALASVIMESEEAETDQHQQPGASASAVGFVDPQSSVTILEAHPVQPNTSFSTTQPDPQPQHTIIRPQSMSQHPVQAPLQQPSSTLTLGPVPQTSPPQVIGQTASLPSTVQSIVPLQYRQPPQDGITLPQSLPSAQVLQAPLSVSQSQPVEQTQPQTSLVPASTESGHSDPASGLSDGNEGRHEGRSMKRPQRRSVRSRSRHEKTAKVKLNVLNISNIGDRVAECQLETHNRKMVTFKFDLDGDNPVEIAEIMVKSDFILESERESFIEQVQLVIQNADQKGKAPQNSQTQVISNSSQQVPEILTSSVPGMPPSLAAQVVHSAGRRFIVSPVPEARLREPLFGTPSANTSFEDTAPAPDPTSASSAPQDQANFASDSNQISVVPPTPGDLAPESSIPPTSQSPYQNPVPSSTATLTVTTTQVSTPTPTTTPQPITGRTSPSPAPTEPPAARRMSLPSPSQTPQIAPGESNGLDQAQASNQQVTQPSATGDSESDVQGKPPGIEDIHALDQKLRSLFKDSSQNSCSQPDGSGETSTTSPPNTISTNTPGFVSGVTPSSLSLNSSGNFAPGAFASIGTPTSYTQTPSTDQSPPRPQIAVGVTQPDGHNSTEEKEPQLEKAFQLGRFQVSVASDQDPPTALDPGVVVSSSSATPSSTSSSSSSSSSPSSLSSPENTIHKSQTPPPRVSPEPVPPTTTVGRFQVTCSTDIKLGRFSVTPAERKSSSVEGPDTAKEPKLTPSSVSITNHYLSSDNDSEPEDEGLRREMNRLKERHKVEIMALQNKQKKEIEELFTRLGKTPSVVVPPTVAMTPGRRRPAKGKGNRPRSGSATQAASFQQGNKQAVQSGSLSQSASEKTSPSETAPSSNNDVQDNTSPVRKENGQSHDQDQTPTTNLPRKGTFTDDLHQLVDNWARDAMNMMPQVKKGSKKNVHEMPRKYSAPGQLCSTGGHIPATINPTAGRKGSIGVANQQFGYTCPNYNMPQWNQVGTISSTAPPAGLQQGFLIPSGAHQTGNNCGSSNLRTTQAS

Target ORF information:

RefSeq Version	XM_021470687.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1a (wnk1a), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470687.1

ORF Insert Sequence:

ATGTCAGAAA ATCTCAACGA CAAGATGGTC AAGTTCCTTT CCCCCCCTTC GAAGAACACC 
AACGGCTCCA GCTCAGACAC TTTGGTGGGT GAGCATCTAG GTGTCGATGT CCGTCGTCGC 
CACCACACCA TGGATCGAGA ACTGCTGAAG GCAGAGCACC GTTTCTTTCG CCGCAGCGTC 
ATCAACGACT CTAATGCTAC AGCGCTTGAG CTGCCCAGCA AGAATGCAAT CCTAACCCAC 
TCCACTGACT CCCATGCCCC TGTCTGTGAA CCTCCTGCCC TAGAAACCAA AGCCGCTTCA 
GTGATCACAC AATCTGCACC AGAAAAAAAA GTGGCTGATG TTGTTGAGAT GGTTATTGAG 
TCAACACCAG TGTTGATTAC AAACGCTGCA CTGCATCTTT CTGCAGAAGC ACCTAGTTTG 
GTTTCAGAGG TAAAGTCTGG AGATGGCATT GATGGAAAAC GAGAGGCTGA GAAAGAGGAG 
GATGAAGATA GTAAAGAAAA GGCGGCACGT GCTGAGGCTG AGTTGCGGGA TGCTAAGAAG 
GAGGAGGAAG ACAATGAGGA AATTGAGACT AATGCTGTTG GGACGTCACC GGATGGCCGC 
TTCTTGAAGT TTGACATCGA GATAGGACGT GGCTCTTTTA AGACGGTCTA CAAGGGACTG 
GATACGGAAA CTACAGTGGA GGTTGCATGG TGTGAGTTAC AGGATCGCAA GTTGTCCAAG 
TCAGAGCGGC AGCGTTTTAA AGAGGAAGCT GGAATGTTGA AGGGTCTTCA GCATCCCAAC 
ATAGTACGCT TTTATGACTC CTGGGAATCA TCCTTGAAAG GGAGGAAGTG TATTGTCTTG 
GTGACTGAAC TCATGACATC TGGGACACTC AAGACGTATC TAAAACGGTT TAAGGAGATG 
AAGATCAAAG TCTTGCGCAG CTGGTGCCGG CAGATTTTGA AGGGCCTACA CTTCCTTCAT 
ACCAGGTCAC CACCTATCAT CCACCGGGAT CTGAAGTGTG ACAACATCTT CATCACTGGG 
CCTACTGGAT CAGTCAAGAT TGGAGATTTG GGCCTTGCGA CCCTCAAGAG GGCCTCCTTT 
GCTAAGAGCG TCATAGGTAC CCCTGAGTTC ATGGCGCCTG AGATGTATGA AGAGAAGTAT 
GACGAGTCGG TGGATGTGTA TGCCTTTGGG ATGTGTATGC TGGAGATGGC TACTTCTGAG 
TACCCGTACT CCGAGTGTCA GAATGCAGCT CAGATCTATC GCAGAGTCAC CAGCGGGGTG 
AAGCCTGGCA GTTTTGACAA AGTGGCAATT CCAGAGGTGA AGGAGATCAT TGAGGGTTGC 
ATTCGTCAGA ATAAAGATGA GAGGTACTGC ATCAAGGATC TCCTAAGCCA TGCCTTCTTC 
CAGGAGGAGA CTGGAGTTCG TGTAGAACTG GCTGAGGAGG ATGATGGAGA GCTTGTGGCT 
ATAAAATTGT TGCTGCGCAT TGAGGATGTT AAGAAACTTA AAGGCAAATA CAAAGAGAAT 
GAAGCCATCG AGTTTTCCTT CGACTTGGAT AAAGACGTCC CAGATGATGT GGCTCAAGAA 
ATGGTTGAAT CAGGCTACAT TTGTGAGGGT GACCACAAAA CCATTGCAAA GGCCATAAAG 
GACAGAGTGT CCCTGATCTC CCGTAAGAGA GAGCAGAGAA AACTGGTGAG AGAAGAACAG 
GAAAAAAGGA AGATGGAGCA GGAGAATGAC GCCGCCCAGC AAACTAACCC CACAGTCAAG 
TCCCCTGGTT CTGCTCTGGC CTCAGTCATA ATGGAGTCTG AAGAAGCAGA AACAGACCAG 
CACCAGCAGC CTGGAGCCTC TGCTTCTGCT GTAGGGTTTG TGGATCCTCA AAGTTCAGTG 
ACCATCCTCG AGGCTCATCC TGTCCAGCCA AACACATCTT TCAGCACCAC ACAGCCTGAT 
CCGCAGCCAC AGCACACAAT TATACGTCCA CAAAGCATGT CGCAGCACCC AGTCCAGGCC 
CCTCTGCAGC AGCCGAGCAG TACTTTGACC CTGGGCCCCG TCCCCCAGAC TTCTCCACCT 
CAGGTGATCG GTCAAACAGC CTCTCTGCCC TCTACTGTTC AATCTATTGT CCCGCTGCAG 
TACCGTCAGC CACCTCAGGA TGGAATCACA CTACCTCAAT CCCTGCCCAG CGCTCAAGTC 
TTACAGGCAC CACTAAGTGT GTCCCAATCA CAACCAGTAG AGCAGACACA ACCTCAAACA 
TCTCTGGTTC CTGCCTCCAC AGAAAGTGGA CACTCTGATC CGGCATCTGG TCTAAGCGAT 
GGGAATGAGG GGAGACACGA GGGTCGCTCT ATGAAACGAC CCCAACGCCG TTCTGTACGC 
AGCCGCTCGC GTCATGAAAA AACAGCTAAG GTCAAGCTCA ATGTGCTAAA TATTTCTAAT 
ATTGGAGACA GAGTCGCTGA ATGTCAGCTG GAAACCCACA ACAGAAAGAT GGTCACATTC 
AAGTTTGATC TGGATGGGGA TAATCCTGTA GAAATCGCTG AAATTATGGT CAAAAGTGAT 
TTTATTTTGG AGAGTGAACG AGAGTCCTTC ATAGAACAGG TTCAGCTGGT CATTCAGAAT 
GCAGATCAGA AAGGAAAAGC TCCCCAAAAT AGCCAAACTC AGGTGATCAG TAATTCGAGC 
CAACAAGTAC CTGAAATTTT AACATCCAGT GTGCCTGGTA TGCCACCTAG CTTAGCAGCA 
CAGGTTGTAC ATTCAGCAGG CAGGCGCTTC ATAGTCAGCC CCGTGCCTGA AGCACGACTT 
CGGGAGCCGT TGTTTGGTAC TCCCTCAGCA AACACATCAT TTGAAGATAC GGCACCAGCA 
CCTGATCCAA CATCAGCCAG CAGTGCTCCG CAGGACCAAG CCAACTTTGC CTCTGATTCA 
AACCAAATTT CTGTGGTACC ACCGACACCT GGAGATCTTG CCCCAGAGAG CTCCATTCCA 
CCTACTTCTC AATCACCATA CCAGAATCCT GTCCCATCAT CCACTGCAAC TTTGACTGTG 
ACCACCACTC AAGTAAGCAC TCCAACACCA ACTACCACTC CGCAACCTAT AACAGGGAGG 
ACGTCTCCTT CACCAGCCCC AACAGAACCA CCAGCAGCAC GTCGTATGTC ACTTCCCAGC 
CCATCCCAGA CACCTCAAAT TGCTCCAGGA GAGAGTAATG GCTTGGATCA AGCACAAGCC 
TCAAATCAGC AAGTAACTCA ACCGAGTGCA ACCGGAGATA GTGAGAGCGA CGTACAGGGG 
AAACCACCTG GAATTGAAGA CATTCATGCT CTAGACCAAA AGCTGCGCTC CCTTTTCAAG 
GACTCATCCC AGAACTCTTG CAGCCAACCT GACGGGTCTG GAGAGACTTC CACAACCTCT 
CCACCCAACA CCATAAGCAC TAACACTCCT GGCTTTGTCT CTGGTGTGAC GCCTTCTAGT 
TTGTCGCTCA ATTCCAGCGG GAACTTTGCA CCTGGTGCTT TCGCCTCCAT TGGTACTCCT 
ACAAGCTACA CCCAGACACC ATCTACCGAC CAGTCCCCTC CAAGGCCACA GATTGCTGTT 
GGTGTAACTC AACCAGATGG ACACAACAGT ACTGAGGAGA AAGAGCCACA ACTTGAGAAA 
GCTTTCCAAC TTGGCCGATT TCAGGTGTCT GTGGCCAGTG ATCAAGACCC ACCAACTGCT 
CTAGACCCAG GAGTGGTGGT TTCATCTTCC TCTGCTACCC CATCTTCAAC ATCATCATCA 
TCATCATCTT CTTCATCACC ATCATCTCTC TCTAGCCCCG AAAACACAAT TCATAAATCT 
CAGACTCCTC CACCCAGAGT CAGCCCTGAA CCCGTACCAC CTACCACTAC AGTTGGCCGC 
TTTCAGGTGA CTTGCAGCAC TGACATCAAA CTTGGTCGCT TCTCAGTGAC ACCAGCAGAA 
AGAAAGTCTT CCTCAGTTGA GGGACCAGAC ACCGCCAAAG AGCCTAAATT GACCCCTTCC 
AGTGTGTCCA TTACCAATCA TTATCTCAGT AGCGACAACG ACTCAGAACC AGAAGATGAG 
GGCTTAAGAC GAGAGATGAA CAGGCTCAAG GAGAGACATA AGGTTGAGAT CATGGCATTG 
CAGAATAAAC AGAAAAAGGA AATTGAGGAG CTCTTCACTC GTCTGGGCAA AACACCATCT 
GTGGTTGTGC CACCCACTGT GGCCATGACG CCTGGTAGAC GGAGACCTGC TAAGGGCAAG 
GGGAACAGGC CAAGAAGTGG TAGTGCCACT CAGGCTGCAT CTTTTCAACA AGGGAACAAA 
CAAGCTGTGC AGAGTGGTTC ACTCAGTCAG TCAGCCAGTG AGAAGACTTC ACCTTCAGAA 
ACAGCACCAA GCTCAAACAA TGATGTGCAA GATAATACAA GTCCAGTGAG AAAAGAAAAC 
GGACAGAGCC ATGACCAGGA CCAAACACCA ACAACTAATC TGCCACGCAA GGGCACATTC 
ACAGATGACC TCCATCAGCT GGTTGATAAC TGGGCCAGAG ATGCCATGAA CATGATGCCA 
CAGGTCAAGA AAGGATCCAA AAAGAATGTT CATGAAATGC CACGTAAATA CTCCGCACCA 
GGTCAGCTTT GTTCCACTGG TGGCCACATC CCAGCGACCA TCAACCCCAC AGCTGGACGG 
AAGGGTTCAA TAGGTGTGGC CAATCAGCAA TTTGGCTACA CTTGTCCCAA CTACAACATG 
CCTCAATGGA ATCAGGTGGG GACAATATCA TCCACCGCTC CACCTGCAGG ACTACAGCAG 
GGTTTCCTTA TCCCATCAGG AGCCCACCAA ACCGGAAATA ACTGCGGATC AAGCAATCTG 
CGCACCACTC AGGCAAGCTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1a ( XM_009298027.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009296302.1 Danio rerio WNK lysine deficient protein kinase 1a (wnk1a), transcript variant X1, mRNA.

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CloneID	ODa49115
Clone ID Related Accession (Same CDS sequence)	XM_009298027.2 , XM_021470686.1
Accession Version	XM_009298027.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009298027.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAAA ATCTCAACGA CAAGATGGTC AAGTTCCTTT CCCCCCCTTC GAAGAACACC 
AACGGCTCCA GCTCAGACAC TTTGGTGGGT GAGCATCTAG GTGTCGATGT CCGTCGTCGC 
CACCACACCA TGGATCGAGA ACTGCTGAAG GCAGAGCACC GTTTCTTTCG CCGCAGCGTC 
ATCAACGACT CTAATGCTAC AGCGCTTGAG CTGCCCAGCA AGAATGCAAT CCTAACCCAC 
TCCACTGACT CCCATGCCCC TGTCTGTGAA CCTCCTGCCC TAGAAACCAA AGCCGCTTCA 
GTGATCACAC AATCTGCACC AGAAAAAAAA GTGGCTGATG TTGTTGAGAT GGTTATTGAG 
TCAACACCAG TGTTGATTAC AAACGCTGCA CTGCATCTTT CTGCAGAAGC ACCTAGTTTG 
GTTTCAGAGG TAAAGTCTGG AGATGGCATT GATGGAAAAC GAGAGGCTGA GAAAGAGGAG 
GATGAAGATA GTAAAGAAAA GGCGGCACGT GCTGAGGCTG AGTTGCGGGA TGCTAAGAAG 
GAGGAGGAAG ACAATGAGGA AATTGAGACT AATGCTGTTG GGACGTCACC GGATGGCCGC 
TTCTTGAAGT TTGACATCGA GATAGGACGT GGCTCTTTTA AGACGGTCTA CAAGGGACTG 
GATACGGAAA CTACAGTGGA GGTTGCATGG TGTGAGTTAC AGGATCGCAA GTTGTCCAAG 
TCAGAGCGGC AGCGTTTTAA AGAGGAAGCT GGAATGTTGA AGGGTCTTCA GCATCCCAAC 
ATAGTACGCT TTTATGACTC CTGGGAATCA TCCTTGAAAG GGAGGAAGTG TATTGTCTTG 
GTGACTGAAC TCATGACATC TGGGACACTC AAGACGTATC TAAAACGGTT TAAGGAGATG 
AAGATCAAAG TCTTGCGCAG CTGGTGCCGG CAGATTTTGA AGGGCCTACA CTTCCTTCAT 
ACCAGGTCAC CACCTATCAT CCACCGGGAT CTGAAGTGTG ACAACATCTT CATCACTGGG 
CCTACTGGAT CAGTCAAGAT TGGAGATTTG GGCCTTGCGA CCCTCAAGAG GGCCTCCTTT 
GCTAAGAGCG TCATAGGTAC CCCTGAGTTC ATGGCGCCTG AGATGTATGA AGAGAAGTAT 
GACGAGTCGG TGGATGTGTA TGCCTTTGGG ATGTGTATGC TGGAGATGGC TACTTCTGAG 
TACCCGTACT CCGAGTGTCA GAATGCAGCT CAGATCTATC GCAGAGTCAC CAGCGGGGTG 
AAGCCTGGCA GTTTTGACAA AGTGGCAATT CCAGAGGTGA AGGAGATCAT TGAGGGTTGC 
ATTCGTCAGA ATAAAGATGA GAGGTACTGC ATCAAGGATC TCCTAAGCCA TGCCTTCTTC 
CAGGAGGAGA CTGGAGTTCG TGTAGAACTG GCTGAGGAGG ATGATGGAGA GCTTGTGGCT 
ATAAAATTGT TGCTGCGCAT TGAGGATGTT AAGAAACTTA AAGGCAAATA CAAAGAGAAT 
GAAGCCATCG AGTTTTCCTT CGACTTGGAT AAAGACGTCC CAGATGATGT GGCTCAAGAA 
ATGGTTGAAT CAGGCTACAT TTGTGAGGGT GACCACAAAA CCATTGCAAA GGCCATAAAG 
GACAGAGTGT CCCTGATCTC CCGTAAGAGA GAGCAGAGAA AACTGGTGAG AGAAGAACAG 
GAAAAAAGGA AGATGGAGCA GGAGAATGAC GCCGCCCAGC AAACTAACCC CACAGTCAAG 
TCCCCTGGTT CTGCTCTGGC CTCAGTCATA ATGGAGTCTG AAGAAGCAGA AACAGACCAG 
CACCAGCAGC CTGGAGCCTC TGCTTCTGCT GTAGGGTTTG TGGATCCTCA AAGTTCAGTG 
ACCATCCTCG AGGCTCATCC TGTCCAGCCA AACACATCTT TCAGCACCAC ACAGCCTGAT 
CCGCAGCCAC AGCACACAAT TATACGTCCA CAAAGCATGT CGCAGCACCC AGTCCAGGCC 
CCTCTGCAGC AGCCGAGCAG TACTTTGACC CTGGGCCCCG TCCCCCAGAC TTCTCCACCT 
CAGGTGATCG GTCAAACAGC CTCTCTGCCC TCTACTGTTC AATCTATTGT CCCGCTGCAG 
TACCGTCAGC CACCTCAGGA TGGAATCACA CTACCTCAAT CCCTGCCCAG CGCTCAAGTC 
TTACAGATAT GGGCATGTCA ACTCAGCCCT CCTGTTTTCT CACCACCTCC AAATGCCGAA 
CAGCTCTATT TTCTATATCA GGCCTGTCAG GCACCACTAA GTGTGTCCCA ATCACAACCA 
GTAGAGCAGA CACAACCTCA AACATCTCTG GTTCCTGCCT CCACAGAAAG TGGACACTCT 
GATCCGGCAT CTGGTCTAAG CGATGGGAAT GAGGGGAGAC ACGAGGGTCG CTCTATGAAA 
CGACCCCAAC GCCGTTCTGT ACGCAGCCGC TCGCGTCATG AAAAAACAGC TAAGGTCAAG 
CTCAATGTGC TAAATATTTC TAATATTGGA GACAGAGTCG CTGAATGTCA GCTGGAAACC 
CACAACAGAA AGATGGTCAC ATTCAAGTTT GATCTGGATG GGGATAATCC TGTAGAAATC 
GCTGAAATTA TGGTCAAAAG TGATTTTATT TTGGAGAGTG AACGAGAGTC CTTCATAGAA 
CAGGTTCAGC TGGTCATTCA GAATGCAGAT CAGAAAGGAA AAGCTCCCCA AAATAGCCAA 
ACTCAGGTGA TCAGTAATTC GAGCCAACAA GTACCTGAAA TTTTAACATC CAGTGTGCCT 
GGTATGCCAC CTAGCTTAGC AGCACAGGTT GTACATTCAG CAGGCAGGCG CTTCATAGTC 
AGCCCCGTGC CTGAAGCACG ACTTCGGGAG CCGTTGTTTG GTACTCCCTC AGCAAACACA 
TCATTTGAAG ATACGGCACC AGCACCTGAT CCAACATCAG CCAGCAGTGC TCCGCAGGAC 
CAAGCCAACT TTGCCTCTGA TTCAAACCAA ATTTCTGTGG TACCACCGAC ACCTGGAGAT 
CTTGCCCCAG AGAGCTCCAT TCCACCTACT TCTCAATCAC CATACCAGAA TCCTGTCCCA 
TCATCCACTG CAACTTTGAC TGTGACCACC ACTCAAGTAA GCACTCCAAC ACCAACTACC 
ACTCCGCAAC CTATAACAGG GAGGACGTCT CCTTCACCAG CCCCAACAGA ACCACCAGCA 
GCACGTCGTA TGTCACTTCC CAGCCCATCC CAGACACCTC AAATTGCTCC AGGAGAGAGT 
AATGGCTTGG ATCAAGCACA AGCCTCAAAT CAGCAAGTAA CTCAACCGAG TGCAACCGGA 
GATAGTGAGA GCGACGTACA GGGGAAACCA CCTGGAATTG AAGACATTCA TGCTCTAGAC 
CAAAAGCTGC GCTCCCTTTT CAAGGACTCA TCCCAGAACT CTTGCAGCCA ACCTGACGGG 
TCTGGAGAGA CTTCCACAAC CTCTCCACCC AACACCATAA GCACTAACAC TCCTGGCTTT 
GTCTCTGGTG TGACGCCTTC TAGTTTGTCG CTCAATTCCA GCGGGAACTT TGCACCTGGT 
GCTTTCGCCT CCATTGGTAC TCCTACAAGC TACACCCAGA CACCATCTAC CGACCAGTCC 
CCTCCAAGGC CACAGATTGC TGTTGGTGTA ACTCAACCAG ATGGACACAA CAGTACTGAG 
GAGAAAGAGC CACAACTTGA GAAAGCTTTC CAACTTGGCC GATTTCAGGT GTCTGTGGCC 
AGTGATCAAG ACCCACCAAC TGCTCTAGAC CCAGGAGTGG TGGTTTCATC TTCCTCTGCT 
ACCCCATCTT CAACATCATC ATCATCATCA TCTTCTTCAT CACCATCATC TCTCTCTAGC 
CCCGAAAACA CAATTCATAA ATCTCAGACT CCTCCACCCA GAGTCAGCCC TGAACCCGTA 
CCACCTACCA CTACAGTTGG CCGCTTTCAG GTGACTTGCA GCACTGACAT CAAACTTGGT 
CGCTTCTCAG TGACACCAGC AGAAAGAAAG TCTTCCTCAG TTGAGGGACC AGACACCGCC 
AAAGAGCCTA AATTGACCCC TTCCAGTGTG TCCATTACCA ATCATTATCT CAGTAGCGAC 
AACGACTCAG AACCAGAAGA TGAGGGCTTA AGACGAGAGA TGAACAGGCT CAAGGAGAGA 
CATAAGGTTG AGATCATGGC ATTGCAGAAT AAACAGAAAA AGGAAATTGA GGAGCTCTTC 
ACTCGTCTGG GCAAAACACC ATCTGTGGTT GTGCCACCCA CTGTGGCCAT GACGCCTGGT 
AGACGGAGAC CTGCTAAGGG CAAGGGGAAC AGGCCAAGAA GTGGTAGTGC CACTCAGGCT 
GCATCTTTTC AACAAGGGAA CAAACAAGCT GTGCAGAGTG GTTCACTCAG TCAGTCAGCC 
AGTGAGAAGA CTTCACCTTC AGAAACAGCA CCAAGCTCAA ACAATGATGT GCAAGATAAT 
ACAAGTCCAG TGAGAAAAGA AAACGGACAG AGCCATGACC AGGACCAAAC ACCAACAACT 
AATCTGCCAC GCAAGGGCAC ATTCACAGAT GACCTCCATC AGCTGGTTGA TAACTGGGCC 
AGAGATGCCA TGAACATGAT GCCACAGGTC AAGAAAGGAT CCAAAAAGAA TGTTCATGAA 
ATGCCACGTA AATACTCCGC ACCAGGTCAG CTTTGTTCCA CTGGTGGCCA CATCCCAGCG 
ACCATCAACC CCACAGCTGG ACGGAAGGGT TCAATAGGTG TGGCCAATCA GCAATTTGGC 
TACACTTGTC CCAACTACAA CATGCCTCAA TGGAATCAGG TGGGGACAAT ATCATCCACC 
GCTCCACCTG CAGGACTACA GCAGGGTTTC CTTATCCCAT CAGGAGCCCA CCAAACCGGA 
AATAACTGCG GATCAAGCAA TCTGCGCACC ACTCAGGCAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009296302.1
CDS	1196..6100
Translation	MSENLNDKMVKFLSPPSKNTNGSSSDTLVGEHLGVDVRRRHHTMDRELLKAEHRFFRRSVINDSNATALELPSKNAILTHSTDSHAPVCEPPALETKAASVITQSAPEKKVADVVEMVIESTPVLITNAALHLSAEAPSLVSEVKSGDGIDGKREAEKEEDEDSKEKAARAEAELRDAKKEEEDNEEIETNAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSKSERQRFKEEAGMLKGLQHPNIVRFYDSWESSLKGRKCIVLVTELMTSGTLKTYLKRFKEMKIKVLRSWCRQILKGLHFLHTRSPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYCIKDLLSHAFFQEETGVRVELAEEDDGELVAIKLLLRIEDVKKLKGKYKENEAIEFSFDLDKDVPDDVAQEMVESGYICEGDHKTIAKAIKDRVSLISRKREQRKLVREEQEKRKMEQENDAAQQTNPTVKSPGSALASVIMESEEAETDQHQQPGASASAVGFVDPQSSVTILEAHPVQPNTSFSTTQPDPQPQHTIIRPQSMSQHPVQAPLQQPSSTLTLGPVPQTSPPQVIGQTASLPSTVQSIVPLQYRQPPQDGITLPQSLPSAQVLQIWACQLSPPVFSPPPNAEQLYFLYQACQAPLSVSQSQPVEQTQPQTSLVPASTESGHSDPASGLSDGNEGRHEGRSMKRPQRRSVRSRSRHEKTAKVKLNVLNISNIGDRVAECQLETHNRKMVTFKFDLDGDNPVEIAEIMVKSDFILESERESFIEQVQLVIQNADQKGKAPQNSQTQVISNSSQQVPEILTSSVPGMPPSLAAQVVHSAGRRFIVSPVPEARLREPLFGTPSANTSFEDTAPAPDPTSASSAPQDQANFASDSNQISVVPPTPGDLAPESSIPPTSQSPYQNPVPSSTATLTVTTTQVSTPTPTTTPQPITGRTSPSPAPTEPPAARRMSLPSPSQTPQIAPGESNGLDQAQASNQQVTQPSATGDSESDVQGKPPGIEDIHALDQKLRSLFKDSSQNSCSQPDGSGETSTTSPPNTISTNTPGFVSGVTPSSLSLNSSGNFAPGAFASIGTPTSYTQTPSTDQSPPRPQIAVGVTQPDGHNSTEEKEPQLEKAFQLGRFQVSVASDQDPPTALDPGVVVSSSSATPSSTSSSSSSSSSPSSLSSPENTIHKSQTPPPRVSPEPVPPTTTVGRFQVTCSTDIKLGRFSVTPAERKSSSVEGPDTAKEPKLTPSSVSITNHYLSSDNDSEPEDEGLRREMNRLKERHKVEIMALQNKQKKEIEELFTRLGKTPSVVVPPTVAMTPGRRRPAKGKGNRPRSGSATQAASFQQGNKQAVQSGSLSQSASEKTSPSETAPSSNNDVQDNTSPVRKENGQSHDQDQTPTTNLPRKGTFTDDLHQLVDNWARDAMNMMPQVKKGSKKNVHEMPRKYSAPGQLCSTGGHIPATINPTAGRKGSIGVANQQFGYTCPNYNMPQWNQVGTISSTAPPAGLQQGFLIPSGAHQTGNNCGSSNLRTTQAS

Target ORF information:

RefSeq Version	XM_009298027.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1a (wnk1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009298027.2

ORF Insert Sequence:

ATGTCAGAAA ATCTCAACGA CAAGATGGTC AAGTTCCTTT CCCCCCCTTC GAAGAACACC 
AACGGCTCCA GCTCAGACAC TTTGGTGGGT GAGCATCTAG GTGTCGATGT CCGTCGTCGC 
CACCACACCA TGGATCGAGA ACTGCTGAAG GCAGAGCACC GTTTCTTTCG CCGCAGCGTC 
ATCAACGACT CTAATGCTAC AGCGCTTGAG CTGCCCAGCA AGAATGCAAT CCTAACCCAC 
TCCACTGACT CCCATGCCCC TGTCTGTGAA CCTCCTGCCC TAGAAACCAA AGCCGCTTCA 
GTGATCACAC AATCTGCACC AGAAAAAAAA GTGGCTGATG TTGTTGAGAT GGTTATTGAG 
TCAACACCAG TGTTGATTAC AAACGCTGCA CTGCATCTTT CTGCAGAAGC ACCTAGTTTG 
GTTTCAGAGG TAAAGTCTGG AGATGGCATT GATGGAAAAC GAGAGGCTGA GAAAGAGGAG 
GATGAAGATA GTAAAGAAAA GGCGGCACGT GCTGAGGCTG AGTTGCGGGA TGCTAAGAAG 
GAGGAGGAAG ACAATGAGGA AATTGAGACT AATGCTGTTG GGACGTCACC GGATGGCCGC 
TTCTTGAAGT TTGACATCGA GATAGGACGT GGCTCTTTTA AGACGGTCTA CAAGGGACTG 
GATACGGAAA CTACAGTGGA GGTTGCATGG TGTGAGTTAC AGGATCGCAA GTTGTCCAAG 
TCAGAGCGGC AGCGTTTTAA AGAGGAAGCT GGAATGTTGA AGGGTCTTCA GCATCCCAAC 
ATAGTACGCT TTTATGACTC CTGGGAATCA TCCTTGAAAG GGAGGAAGTG TATTGTCTTG 
GTGACTGAAC TCATGACATC TGGGACACTC AAGACGTATC TAAAACGGTT TAAGGAGATG 
AAGATCAAAG TCTTGCGCAG CTGGTGCCGG CAGATTTTGA AGGGCCTACA CTTCCTTCAT 
ACCAGGTCAC CACCTATCAT CCACCGGGAT CTGAAGTGTG ACAACATCTT CATCACTGGG 
CCTACTGGAT CAGTCAAGAT TGGAGATTTG GGCCTTGCGA CCCTCAAGAG GGCCTCCTTT 
GCTAAGAGCG TCATAGGTAC CCCTGAGTTC ATGGCGCCTG AGATGTATGA AGAGAAGTAT 
GACGAGTCGG TGGATGTGTA TGCCTTTGGG ATGTGTATGC TGGAGATGGC TACTTCTGAG 
TACCCGTACT CCGAGTGTCA GAATGCAGCT CAGATCTATC GCAGAGTCAC CAGCGGGGTG 
AAGCCTGGCA GTTTTGACAA AGTGGCAATT CCAGAGGTGA AGGAGATCAT TGAGGGTTGC 
ATTCGTCAGA ATAAAGATGA GAGGTACTGC ATCAAGGATC TCCTAAGCCA TGCCTTCTTC 
CAGGAGGAGA CTGGAGTTCG TGTAGAACTG GCTGAGGAGG ATGATGGAGA GCTTGTGGCT 
ATAAAATTGT TGCTGCGCAT TGAGGATGTT AAGAAACTTA AAGGCAAATA CAAAGAGAAT 
GAAGCCATCG AGTTTTCCTT CGACTTGGAT AAAGACGTCC CAGATGATGT GGCTCAAGAA 
ATGGTTGAAT CAGGCTACAT TTGTGAGGGT GACCACAAAA CCATTGCAAA GGCCATAAAG 
GACAGAGTGT CCCTGATCTC CCGTAAGAGA GAGCAGAGAA AACTGGTGAG AGAAGAACAG 
GAAAAAAGGA AGATGGAGCA GGAGAATGAC GCCGCCCAGC AAACTAACCC CACAGTCAAG 
TCCCCTGGTT CTGCTCTGGC CTCAGTCATA ATGGAGTCTG AAGAAGCAGA AACAGACCAG 
CACCAGCAGC CTGGAGCCTC TGCTTCTGCT GTAGGGTTTG TGGATCCTCA AAGTTCAGTG 
ACCATCCTCG AGGCTCATCC TGTCCAGCCA AACACATCTT TCAGCACCAC ACAGCCTGAT 
CCGCAGCCAC AGCACACAAT TATACGTCCA CAAAGCATGT CGCAGCACCC AGTCCAGGCC 
CCTCTGCAGC AGCCGAGCAG TACTTTGACC CTGGGCCCCG TCCCCCAGAC TTCTCCACCT 
CAGGTGATCG GTCAAACAGC CTCTCTGCCC TCTACTGTTC AATCTATTGT CCCGCTGCAG 
TACCGTCAGC CACCTCAGGA TGGAATCACA CTACCTCAAT CCCTGCCCAG CGCTCAAGTC 
TTACAGATAT GGGCATGTCA ACTCAGCCCT CCTGTTTTCT CACCACCTCC AAATGCCGAA 
CAGCTCTATT TTCTATATCA GGCCTGTCAG GCACCACTAA GTGTGTCCCA ATCACAACCA 
GTAGAGCAGA CACAACCTCA AACATCTCTG GTTCCTGCCT CCACAGAAAG TGGACACTCT 
GATCCGGCAT CTGGTCTAAG CGATGGGAAT GAGGGGAGAC ACGAGGGTCG CTCTATGAAA 
CGACCCCAAC GCCGTTCTGT ACGCAGCCGC TCGCGTCATG AAAAAACAGC TAAGGTCAAG 
CTCAATGTGC TAAATATTTC TAATATTGGA GACAGAGTCG CTGAATGTCA GCTGGAAACC 
CACAACAGAA AGATGGTCAC ATTCAAGTTT GATCTGGATG GGGATAATCC TGTAGAAATC 
GCTGAAATTA TGGTCAAAAG TGATTTTATT TTGGAGAGTG AACGAGAGTC CTTCATAGAA 
CAGGTTCAGC TGGTCATTCA GAATGCAGAT CAGAAAGGAA AAGCTCCCCA AAATAGCCAA 
ACTCAGGTGA TCAGTAATTC GAGCCAACAA GTACCTGAAA TTTTAACATC CAGTGTGCCT 
GGTATGCCAC CTAGCTTAGC AGCACAGGTT GTACATTCAG CAGGCAGGCG CTTCATAGTC 
AGCCCCGTGC CTGAAGCACG ACTTCGGGAG CCGTTGTTTG GTACTCCCTC AGCAAACACA 
TCATTTGAAG ATACGGCACC AGCACCTGAT CCAACATCAG CCAGCAGTGC TCCGCAGGAC 
CAAGCCAACT TTGCCTCTGA TTCAAACCAA ATTTCTGTGG TACCACCGAC ACCTGGAGAT 
CTTGCCCCAG AGAGCTCCAT TCCACCTACT TCTCAATCAC CATACCAGAA TCCTGTCCCA 
TCATCCACTG CAACTTTGAC TGTGACCACC ACTCAAGTAA GCACTCCAAC ACCAACTACC 
ACTCCGCAAC CTATAACAGG GAGGACGTCT CCTTCACCAG CCCCAACAGA ACCACCAGCA 
GCACGTCGTA TGTCACTTCC CAGCCCATCC CAGACACCTC AAATTGCTCC AGGAGAGAGT 
AATGGCTTGG ATCAAGCACA AGCCTCAAAT CAGCAAGTAA CTCAACCGAG TGCAACCGGA 
GATAGTGAGA GCGACGTACA GGGGAAACCA CCTGGAATTG AAGACATTCA TGCTCTAGAC 
CAAAAGCTGC GCTCCCTTTT CAAGGACTCA TCCCAGAACT CTTGCAGCCA ACCTGACGGG 
TCTGGAGAGA CTTCCACAAC CTCTCCACCC AACACCATAA GCACTAACAC TCCTGGCTTT 
GTCTCTGGTG TGACGCCTTC TAGTTTGTCG CTCAATTCCA GCGGGAACTT TGCACCTGGT 
GCTTTCGCCT CCATTGGTAC TCCTACAAGC TACACCCAGA CACCATCTAC CGACCAGTCC 
CCTCCAAGGC CACAGATTGC TGTTGGTGTA ACTCAACCAG ATGGACACAA CAGTACTGAG 
GAGAAAGAGC CACAACTTGA GAAAGCTTTC CAACTTGGCC GATTTCAGGT GTCTGTGGCC 
AGTGATCAAG ACCCACCAAC TGCTCTAGAC CCAGGAGTGG TGGTTTCATC TTCCTCTGCT 
ACCCCATCTT CAACATCATC ATCATCATCA TCTTCTTCAT CACCATCATC TCTCTCTAGC 
CCCGAAAACA CAATTCATAA ATCTCAGACT CCTCCACCCA GAGTCAGCCC TGAACCCGTA 
CCACCTACCA CTACAGTTGG CCGCTTTCAG GTGACTTGCA GCACTGACAT CAAACTTGGT 
CGCTTCTCAG TGACACCAGC AGAAAGAAAG TCTTCCTCAG TTGAGGGACC AGACACCGCC 
AAAGAGCCTA AATTGACCCC TTCCAGTGTG TCCATTACCA ATCATTATCT CAGTAGCGAC 
AACGACTCAG AACCAGAAGA TGAGGGCTTA AGACGAGAGA TGAACAGGCT CAAGGAGAGA 
CATAAGGTTG AGATCATGGC ATTGCAGAAT AAACAGAAAA AGGAAATTGA GGAGCTCTTC 
ACTCGTCTGG GCAAAACACC ATCTGTGGTT GTGCCACCCA CTGTGGCCAT GACGCCTGGT 
AGACGGAGAC CTGCTAAGGG CAAGGGGAAC AGGCCAAGAA GTGGTAGTGC CACTCAGGCT 
GCATCTTTTC AACAAGGGAA CAAACAAGCT GTGCAGAGTG GTTCACTCAG TCAGTCAGCC 
AGTGAGAAGA CTTCACCTTC AGAAACAGCA CCAAGCTCAA ACAATGATGT GCAAGATAAT 
ACAAGTCCAG TGAGAAAAGA AAACGGACAG AGCCATGACC AGGACCAAAC ACCAACAACT 
AATCTGCCAC GCAAGGGCAC ATTCACAGAT GACCTCCATC AGCTGGTTGA TAACTGGGCC 
AGAGATGCCA TGAACATGAT GCCACAGGTC AAGAAAGGAT CCAAAAAGAA TGTTCATGAA 
ATGCCACGTA AATACTCCGC ACCAGGTCAG CTTTGTTCCA CTGGTGGCCA CATCCCAGCG 
ACCATCAACC CCACAGCTGG ACGGAAGGGT TCAATAGGTG TGGCCAATCA GCAATTTGGC 
TACACTTGTC CCAACTACAA CATGCCTCAA TGGAATCAGG TGGGGACAAT ATCATCCACC 
GCTCCACCTG CAGGACTACA GCAGGGTTTC CTTATCCCAT CAGGAGCCCA CCAAACCGGA 
AATAACTGCG GATCAAGCAA TCTGCGCACC ACTCAGGCAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

wnk1a ( XM_021470686.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326361.1 Danio rerio WNK lysine deficient protein kinase 1a (wnk1a), transcript variant X1, mRNA.

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CloneID	ODa49115
Clone ID Related Accession (Same CDS sequence)	XM_009298027.2 , XM_021470686.1
Accession Version	XM_021470686.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4905bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	serine/threonine-protein kinase WNK1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTCAGAAA ATCTCAACGA CAAGATGGTC AAGTTCCTTT CCCCCCCTTC GAAGAACACC 
AACGGCTCCA GCTCAGACAC TTTGGTGGGT GAGCATCTAG GTGTCGATGT CCGTCGTCGC 
CACCACACCA TGGATCGAGA ACTGCTGAAG GCAGAGCACC GTTTCTTTCG CCGCAGCGTC 
ATCAACGACT CTAATGCTAC AGCGCTTGAG CTGCCCAGCA AGAATGCAAT CCTAACCCAC 
TCCACTGACT CCCATGCCCC TGTCTGTGAA CCTCCTGCCC TAGAAACCAA AGCCGCTTCA 
GTGATCACAC AATCTGCACC AGAAAAAAAA GTGGCTGATG TTGTTGAGAT GGTTATTGAG 
TCAACACCAG TGTTGATTAC AAACGCTGCA CTGCATCTTT CTGCAGAAGC ACCTAGTTTG 
GTTTCAGAGG TAAAGTCTGG AGATGGCATT GATGGAAAAC GAGAGGCTGA GAAAGAGGAG 
GATGAAGATA GTAAAGAAAA GGCGGCACGT GCTGAGGCTG AGTTGCGGGA TGCTAAGAAG 
GAGGAGGAAG ACAATGAGGA AATTGAGACT AATGCTGTTG GGACGTCACC GGATGGCCGC 
TTCTTGAAGT TTGACATCGA GATAGGACGT GGCTCTTTTA AGACGGTCTA CAAGGGACTG 
GATACGGAAA CTACAGTGGA GGTTGCATGG TGTGAGTTAC AGGATCGCAA GTTGTCCAAG 
TCAGAGCGGC AGCGTTTTAA AGAGGAAGCT GGAATGTTGA AGGGTCTTCA GCATCCCAAC 
ATAGTACGCT TTTATGACTC CTGGGAATCA TCCTTGAAAG GGAGGAAGTG TATTGTCTTG 
GTGACTGAAC TCATGACATC TGGGACACTC AAGACGTATC TAAAACGGTT TAAGGAGATG 
AAGATCAAAG TCTTGCGCAG CTGGTGCCGG CAGATTTTGA AGGGCCTACA CTTCCTTCAT 
ACCAGGTCAC CACCTATCAT CCACCGGGAT CTGAAGTGTG ACAACATCTT CATCACTGGG 
CCTACTGGAT CAGTCAAGAT TGGAGATTTG GGCCTTGCGA CCCTCAAGAG GGCCTCCTTT 
GCTAAGAGCG TCATAGGTAC CCCTGAGTTC ATGGCGCCTG AGATGTATGA AGAGAAGTAT 
GACGAGTCGG TGGATGTGTA TGCCTTTGGG ATGTGTATGC TGGAGATGGC TACTTCTGAG 
TACCCGTACT CCGAGTGTCA GAATGCAGCT CAGATCTATC GCAGAGTCAC CAGCGGGGTG 
AAGCCTGGCA GTTTTGACAA AGTGGCAATT CCAGAGGTGA AGGAGATCAT TGAGGGTTGC 
ATTCGTCAGA ATAAAGATGA GAGGTACTGC ATCAAGGATC TCCTAAGCCA TGCCTTCTTC 
CAGGAGGAGA CTGGAGTTCG TGTAGAACTG GCTGAGGAGG ATGATGGAGA GCTTGTGGCT 
ATAAAATTGT TGCTGCGCAT TGAGGATGTT AAGAAACTTA AAGGCAAATA CAAAGAGAAT 
GAAGCCATCG AGTTTTCCTT CGACTTGGAT AAAGACGTCC CAGATGATGT GGCTCAAGAA 
ATGGTTGAAT CAGGCTACAT TTGTGAGGGT GACCACAAAA CCATTGCAAA GGCCATAAAG 
GACAGAGTGT CCCTGATCTC CCGTAAGAGA GAGCAGAGAA AACTGGTGAG AGAAGAACAG 
GAAAAAAGGA AGATGGAGCA GGAGAATGAC GCCGCCCAGC AAACTAACCC CACAGTCAAG 
TCCCCTGGTT CTGCTCTGGC CTCAGTCATA ATGGAGTCTG AAGAAGCAGA AACAGACCAG 
CACCAGCAGC CTGGAGCCTC TGCTTCTGCT GTAGGGTTTG TGGATCCTCA AAGTTCAGTG 
ACCATCCTCG AGGCTCATCC TGTCCAGCCA AACACATCTT TCAGCACCAC ACAGCCTGAT 
CCGCAGCCAC AGCACACAAT TATACGTCCA CAAAGCATGT CGCAGCACCC AGTCCAGGCC 
CCTCTGCAGC AGCCGAGCAG TACTTTGACC CTGGGCCCCG TCCCCCAGAC TTCTCCACCT 
CAGGTGATCG GTCAAACAGC CTCTCTGCCC TCTACTGTTC AATCTATTGT CCCGCTGCAG 
TACCGTCAGC CACCTCAGGA TGGAATCACA CTACCTCAAT CCCTGCCCAG CGCTCAAGTC 
TTACAGATAT GGGCATGTCA ACTCAGCCCT CCTGTTTTCT CACCACCTCC AAATGCCGAA 
CAGCTCTATT TTCTATATCA GGCCTGTCAG GCACCACTAA GTGTGTCCCA ATCACAACCA 
GTAGAGCAGA CACAACCTCA AACATCTCTG GTTCCTGCCT CCACAGAAAG TGGACACTCT 
GATCCGGCAT CTGGTCTAAG CGATGGGAAT GAGGGGAGAC ACGAGGGTCG CTCTATGAAA 
CGACCCCAAC GCCGTTCTGT ACGCAGCCGC TCGCGTCATG AAAAAACAGC TAAGGTCAAG 
CTCAATGTGC TAAATATTTC TAATATTGGA GACAGAGTCG CTGAATGTCA GCTGGAAACC 
CACAACAGAA AGATGGTCAC ATTCAAGTTT GATCTGGATG GGGATAATCC TGTAGAAATC 
GCTGAAATTA TGGTCAAAAG TGATTTTATT TTGGAGAGTG AACGAGAGTC CTTCATAGAA 
CAGGTTCAGC TGGTCATTCA GAATGCAGAT CAGAAAGGAA AAGCTCCCCA AAATAGCCAA 
ACTCAGGTGA TCAGTAATTC GAGCCAACAA GTACCTGAAA TTTTAACATC CAGTGTGCCT 
GGTATGCCAC CTAGCTTAGC AGCACAGGTT GTACATTCAG CAGGCAGGCG CTTCATAGTC 
AGCCCCGTGC CTGAAGCACG ACTTCGGGAG CCGTTGTTTG GTACTCCCTC AGCAAACACA 
TCATTTGAAG ATACGGCACC AGCACCTGAT CCAACATCAG CCAGCAGTGC TCCGCAGGAC 
CAAGCCAACT TTGCCTCTGA TTCAAACCAA ATTTCTGTGG TACCACCGAC ACCTGGAGAT 
CTTGCCCCAG AGAGCTCCAT TCCACCTACT TCTCAATCAC CATACCAGAA TCCTGTCCCA 
TCATCCACTG CAACTTTGAC TGTGACCACC ACTCAAGTAA GCACTCCAAC ACCAACTACC 
ACTCCGCAAC CTATAACAGG GAGGACGTCT CCTTCACCAG CCCCAACAGA ACCACCAGCA 
GCACGTCGTA TGTCACTTCC CAGCCCATCC CAGACACCTC AAATTGCTCC AGGAGAGAGT 
AATGGCTTGG ATCAAGCACA AGCCTCAAAT CAGCAAGTAA CTCAACCGAG TGCAACCGGA 
GATAGTGAGA GCGACGTACA GGGGAAACCA CCTGGAATTG AAGACATTCA TGCTCTAGAC 
CAAAAGCTGC GCTCCCTTTT CAAGGACTCA TCCCAGAACT CTTGCAGCCA ACCTGACGGG 
TCTGGAGAGA CTTCCACAAC CTCTCCACCC AACACCATAA GCACTAACAC TCCTGGCTTT 
GTCTCTGGTG TGACGCCTTC TAGTTTGTCG CTCAATTCCA GCGGGAACTT TGCACCTGGT 
GCTTTCGCCT CCATTGGTAC TCCTACAAGC TACACCCAGA CACCATCTAC CGACCAGTCC 
CCTCCAAGGC CACAGATTGC TGTTGGTGTA ACTCAACCAG ATGGACACAA CAGTACTGAG 
GAGAAAGAGC CACAACTTGA GAAAGCTTTC CAACTTGGCC GATTTCAGGT GTCTGTGGCC 
AGTGATCAAG ACCCACCAAC TGCTCTAGAC CCAGGAGTGG TGGTTTCATC TTCCTCTGCT 
ACCCCATCTT CAACATCATC ATCATCATCA TCTTCTTCAT CACCATCATC TCTCTCTAGC 
CCCGAAAACA CAATTCATAA ATCTCAGACT CCTCCACCCA GAGTCAGCCC TGAACCCGTA 
CCACCTACCA CTACAGTTGG CCGCTTTCAG GTGACTTGCA GCACTGACAT CAAACTTGGT 
CGCTTCTCAG TGACACCAGC AGAAAGAAAG TCTTCCTCAG TTGAGGGACC AGACACCGCC 
AAAGAGCCTA AATTGACCCC TTCCAGTGTG TCCATTACCA ATCATTATCT CAGTAGCGAC 
AACGACTCAG AACCAGAAGA TGAGGGCTTA AGACGAGAGA TGAACAGGCT CAAGGAGAGA 
CATAAGGTTG AGATCATGGC ATTGCAGAAT AAACAGAAAA AGGAAATTGA GGAGCTCTTC 
ACTCGTCTGG GCAAAACACC ATCTGTGGTT GTGCCACCCA CTGTGGCCAT GACGCCTGGT 
AGACGGAGAC CTGCTAAGGG CAAGGGGAAC AGGCCAAGAA GTGGTAGTGC CACTCAGGCT 
GCATCTTTTC AACAAGGGAA CAAACAAGCT GTGCAGAGTG GTTCACTCAG TCAGTCAGCC 
AGTGAGAAGA CTTCACCTTC AGAAACAGCA CCAAGCTCAA ACAATGATGT GCAAGATAAT 
ACAAGTCCAG TGAGAAAAGA AAACGGACAG AGCCATGACC AGGACCAAAC ACCAACAACT 
AATCTGCCAC GCAAGGGCAC ATTCACAGAT GACCTCCATC AGCTGGTTGA TAACTGGGCC 
AGAGATGCCA TGAACATGAT GCCACAGGTC AAGAAAGGAT CCAAAAAGAA TGTTCATGAA 
ATGCCACGTA AATACTCCGC ACCAGGTCAG CTTTGTTCCA CTGGTGGCCA CATCCCAGCG 
ACCATCAACC CCACAGCTGG ACGGAAGGGT TCAATAGGTG TGGCCAATCA GCAATTTGGC 
TACACTTGTC CCAACTACAA CATGCCTCAA TGGAATCAGG TGGGGACAAT ATCATCCACC 
GCTCCACCTG CAGGACTACA GCAGGGTTTC CTTATCCCAT CAGGAGCCCA CCAAACCGGA 
AATAACTGCG GATCAAGCAA TCTGCGCACC ACTCAGGCAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326361.1
CDS	1193..6097
Translation	MSENLNDKMVKFLSPPSKNTNGSSSDTLVGEHLGVDVRRRHHTMDRELLKAEHRFFRRSVINDSNATALELPSKNAILTHSTDSHAPVCEPPALETKAASVITQSAPEKKVADVVEMVIESTPVLITNAALHLSAEAPSLVSEVKSGDGIDGKREAEKEEDEDSKEKAARAEAELRDAKKEEEDNEEIETNAVGTSPDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLSKSERQRFKEEAGMLKGLQHPNIVRFYDSWESSLKGRKCIVLVTELMTSGTLKTYLKRFKEMKIKVLRSWCRQILKGLHFLHTRSPPIIHRDLKCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEYPYSECQNAAQIYRRVTSGVKPGSFDKVAIPEVKEIIEGCIRQNKDERYCIKDLLSHAFFQEETGVRVELAEEDDGELVAIKLLLRIEDVKKLKGKYKENEAIEFSFDLDKDVPDDVAQEMVESGYICEGDHKTIAKAIKDRVSLISRKREQRKLVREEQEKRKMEQENDAAQQTNPTVKSPGSALASVIMESEEAETDQHQQPGASASAVGFVDPQSSVTILEAHPVQPNTSFSTTQPDPQPQHTIIRPQSMSQHPVQAPLQQPSSTLTLGPVPQTSPPQVIGQTASLPSTVQSIVPLQYRQPPQDGITLPQSLPSAQVLQIWACQLSPPVFSPPPNAEQLYFLYQACQAPLSVSQSQPVEQTQPQTSLVPASTESGHSDPASGLSDGNEGRHEGRSMKRPQRRSVRSRSRHEKTAKVKLNVLNISNIGDRVAECQLETHNRKMVTFKFDLDGDNPVEIAEIMVKSDFILESERESFIEQVQLVIQNADQKGKAPQNSQTQVISNSSQQVPEILTSSVPGMPPSLAAQVVHSAGRRFIVSPVPEARLREPLFGTPSANTSFEDTAPAPDPTSASSAPQDQANFASDSNQISVVPPTPGDLAPESSIPPTSQSPYQNPVPSSTATLTVTTTQVSTPTPTTTPQPITGRTSPSPAPTEPPAARRMSLPSPSQTPQIAPGESNGLDQAQASNQQVTQPSATGDSESDVQGKPPGIEDIHALDQKLRSLFKDSSQNSCSQPDGSGETSTTSPPNTISTNTPGFVSGVTPSSLSLNSSGNFAPGAFASIGTPTSYTQTPSTDQSPPRPQIAVGVTQPDGHNSTEEKEPQLEKAFQLGRFQVSVASDQDPPTALDPGVVVSSSSATPSSTSSSSSSSSSPSSLSSPENTIHKSQTPPPRVSPEPVPPTTTVGRFQVTCSTDIKLGRFSVTPAERKSSSVEGPDTAKEPKLTPSSVSITNHYLSSDNDSEPEDEGLRREMNRLKERHKVEIMALQNKQKKEIEELFTRLGKTPSVVVPPTVAMTPGRRRPAKGKGNRPRSGSATQAASFQQGNKQAVQSGSLSQSASEKTSPSETAPSSNNDVQDNTSPVRKENGQSHDQDQTPTTNLPRKGTFTDDLHQLVDNWARDAMNMMPQVKKGSKKNVHEMPRKYSAPGQLCSTGGHIPATINPTAGRKGSIGVANQQFGYTCPNYNMPQWNQVGTISSTAPPAGLQQGFLIPSGAHQTGNNCGSSNLRTTQAS

Target ORF information:

RefSeq Version	XM_021470686.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio WNK lysine deficient protein kinase 1a (wnk1a), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470686.1

ORF Insert Sequence:

ATGTCAGAAA ATCTCAACGA CAAGATGGTC AAGTTCCTTT CCCCCCCTTC GAAGAACACC 
AACGGCTCCA GCTCAGACAC TTTGGTGGGT GAGCATCTAG GTGTCGATGT CCGTCGTCGC 
CACCACACCA TGGATCGAGA ACTGCTGAAG GCAGAGCACC GTTTCTTTCG CCGCAGCGTC 
ATCAACGACT CTAATGCTAC AGCGCTTGAG CTGCCCAGCA AGAATGCAAT CCTAACCCAC 
TCCACTGACT CCCATGCCCC TGTCTGTGAA CCTCCTGCCC TAGAAACCAA AGCCGCTTCA 
GTGATCACAC AATCTGCACC AGAAAAAAAA GTGGCTGATG TTGTTGAGAT GGTTATTGAG 
TCAACACCAG TGTTGATTAC AAACGCTGCA CTGCATCTTT CTGCAGAAGC ACCTAGTTTG 
GTTTCAGAGG TAAAGTCTGG AGATGGCATT GATGGAAAAC GAGAGGCTGA GAAAGAGGAG 
GATGAAGATA GTAAAGAAAA GGCGGCACGT GCTGAGGCTG AGTTGCGGGA TGCTAAGAAG 
GAGGAGGAAG ACAATGAGGA AATTGAGACT AATGCTGTTG GGACGTCACC GGATGGCCGC 
TTCTTGAAGT TTGACATCGA GATAGGACGT GGCTCTTTTA AGACGGTCTA CAAGGGACTG 
GATACGGAAA CTACAGTGGA GGTTGCATGG TGTGAGTTAC AGGATCGCAA GTTGTCCAAG 
TCAGAGCGGC AGCGTTTTAA AGAGGAAGCT GGAATGTTGA AGGGTCTTCA GCATCCCAAC 
ATAGTACGCT TTTATGACTC CTGGGAATCA TCCTTGAAAG GGAGGAAGTG TATTGTCTTG 
GTGACTGAAC TCATGACATC TGGGACACTC AAGACGTATC TAAAACGGTT TAAGGAGATG 
AAGATCAAAG TCTTGCGCAG CTGGTGCCGG CAGATTTTGA AGGGCCTACA CTTCCTTCAT 
ACCAGGTCAC CACCTATCAT CCACCGGGAT CTGAAGTGTG ACAACATCTT CATCACTGGG 
CCTACTGGAT CAGTCAAGAT TGGAGATTTG GGCCTTGCGA CCCTCAAGAG GGCCTCCTTT 
GCTAAGAGCG TCATAGGTAC CCCTGAGTTC ATGGCGCCTG AGATGTATGA AGAGAAGTAT 
GACGAGTCGG TGGATGTGTA TGCCTTTGGG ATGTGTATGC TGGAGATGGC TACTTCTGAG 
TACCCGTACT CCGAGTGTCA GAATGCAGCT CAGATCTATC GCAGAGTCAC CAGCGGGGTG 
AAGCCTGGCA GTTTTGACAA AGTGGCAATT CCAGAGGTGA AGGAGATCAT TGAGGGTTGC 
ATTCGTCAGA ATAAAGATGA GAGGTACTGC ATCAAGGATC TCCTAAGCCA TGCCTTCTTC 
CAGGAGGAGA CTGGAGTTCG TGTAGAACTG GCTGAGGAGG ATGATGGAGA GCTTGTGGCT 
ATAAAATTGT TGCTGCGCAT TGAGGATGTT AAGAAACTTA AAGGCAAATA CAAAGAGAAT 
GAAGCCATCG AGTTTTCCTT CGACTTGGAT AAAGACGTCC CAGATGATGT GGCTCAAGAA 
ATGGTTGAAT CAGGCTACAT TTGTGAGGGT GACCACAAAA CCATTGCAAA GGCCATAAAG 
GACAGAGTGT CCCTGATCTC CCGTAAGAGA GAGCAGAGAA AACTGGTGAG AGAAGAACAG 
GAAAAAAGGA AGATGGAGCA GGAGAATGAC GCCGCCCAGC AAACTAACCC CACAGTCAAG 
TCCCCTGGTT CTGCTCTGGC CTCAGTCATA ATGGAGTCTG AAGAAGCAGA AACAGACCAG 
CACCAGCAGC CTGGAGCCTC TGCTTCTGCT GTAGGGTTTG TGGATCCTCA AAGTTCAGTG 
ACCATCCTCG AGGCTCATCC TGTCCAGCCA AACACATCTT TCAGCACCAC ACAGCCTGAT 
CCGCAGCCAC AGCACACAAT TATACGTCCA CAAAGCATGT CGCAGCACCC AGTCCAGGCC 
CCTCTGCAGC AGCCGAGCAG TACTTTGACC CTGGGCCCCG TCCCCCAGAC TTCTCCACCT 
CAGGTGATCG GTCAAACAGC CTCTCTGCCC TCTACTGTTC AATCTATTGT CCCGCTGCAG 
TACCGTCAGC CACCTCAGGA TGGAATCACA CTACCTCAAT CCCTGCCCAG CGCTCAAGTC 
TTACAGATAT GGGCATGTCA ACTCAGCCCT CCTGTTTTCT CACCACCTCC AAATGCCGAA 
CAGCTCTATT TTCTATATCA GGCCTGTCAG GCACCACTAA GTGTGTCCCA ATCACAACCA 
GTAGAGCAGA CACAACCTCA AACATCTCTG GTTCCTGCCT CCACAGAAAG TGGACACTCT 
GATCCGGCAT CTGGTCTAAG CGATGGGAAT GAGGGGAGAC ACGAGGGTCG CTCTATGAAA 
CGACCCCAAC GCCGTTCTGT ACGCAGCCGC TCGCGTCATG AAAAAACAGC TAAGGTCAAG 
CTCAATGTGC TAAATATTTC TAATATTGGA GACAGAGTCG CTGAATGTCA GCTGGAAACC 
CACAACAGAA AGATGGTCAC ATTCAAGTTT GATCTGGATG GGGATAATCC TGTAGAAATC 
GCTGAAATTA TGGTCAAAAG TGATTTTATT TTGGAGAGTG AACGAGAGTC CTTCATAGAA 
CAGGTTCAGC TGGTCATTCA GAATGCAGAT CAGAAAGGAA AAGCTCCCCA AAATAGCCAA 
ACTCAGGTGA TCAGTAATTC GAGCCAACAA GTACCTGAAA TTTTAACATC CAGTGTGCCT 
GGTATGCCAC CTAGCTTAGC AGCACAGGTT GTACATTCAG CAGGCAGGCG CTTCATAGTC 
AGCCCCGTGC CTGAAGCACG ACTTCGGGAG CCGTTGTTTG GTACTCCCTC AGCAAACACA 
TCATTTGAAG ATACGGCACC AGCACCTGAT CCAACATCAG CCAGCAGTGC TCCGCAGGAC 
CAAGCCAACT TTGCCTCTGA TTCAAACCAA ATTTCTGTGG TACCACCGAC ACCTGGAGAT 
CTTGCCCCAG AGAGCTCCAT TCCACCTACT TCTCAATCAC CATACCAGAA TCCTGTCCCA 
TCATCCACTG CAACTTTGAC TGTGACCACC ACTCAAGTAA GCACTCCAAC ACCAACTACC 
ACTCCGCAAC CTATAACAGG GAGGACGTCT CCTTCACCAG CCCCAACAGA ACCACCAGCA 
GCACGTCGTA TGTCACTTCC CAGCCCATCC CAGACACCTC AAATTGCTCC AGGAGAGAGT 
AATGGCTTGG ATCAAGCACA AGCCTCAAAT CAGCAAGTAA CTCAACCGAG TGCAACCGGA 
GATAGTGAGA GCGACGTACA GGGGAAACCA CCTGGAATTG AAGACATTCA TGCTCTAGAC 
CAAAAGCTGC GCTCCCTTTT CAAGGACTCA TCCCAGAACT CTTGCAGCCA ACCTGACGGG 
TCTGGAGAGA CTTCCACAAC CTCTCCACCC AACACCATAA GCACTAACAC TCCTGGCTTT 
GTCTCTGGTG TGACGCCTTC TAGTTTGTCG CTCAATTCCA GCGGGAACTT TGCACCTGGT 
GCTTTCGCCT CCATTGGTAC TCCTACAAGC TACACCCAGA CACCATCTAC CGACCAGTCC 
CCTCCAAGGC CACAGATTGC TGTTGGTGTA ACTCAACCAG ATGGACACAA CAGTACTGAG 
GAGAAAGAGC CACAACTTGA GAAAGCTTTC CAACTTGGCC GATTTCAGGT GTCTGTGGCC 
AGTGATCAAG ACCCACCAAC TGCTCTAGAC CCAGGAGTGG TGGTTTCATC TTCCTCTGCT 
ACCCCATCTT CAACATCATC ATCATCATCA TCTTCTTCAT CACCATCATC TCTCTCTAGC 
CCCGAAAACA CAATTCATAA ATCTCAGACT CCTCCACCCA GAGTCAGCCC TGAACCCGTA 
CCACCTACCA CTACAGTTGG CCGCTTTCAG GTGACTTGCA GCACTGACAT CAAACTTGGT 
CGCTTCTCAG TGACACCAGC AGAAAGAAAG TCTTCCTCAG TTGAGGGACC AGACACCGCC 
AAAGAGCCTA AATTGACCCC TTCCAGTGTG TCCATTACCA ATCATTATCT CAGTAGCGAC 
AACGACTCAG AACCAGAAGA TGAGGGCTTA AGACGAGAGA TGAACAGGCT CAAGGAGAGA 
CATAAGGTTG AGATCATGGC ATTGCAGAAT AAACAGAAAA AGGAAATTGA GGAGCTCTTC 
ACTCGTCTGG GCAAAACACC ATCTGTGGTT GTGCCACCCA CTGTGGCCAT GACGCCTGGT 
AGACGGAGAC CTGCTAAGGG CAAGGGGAAC AGGCCAAGAA GTGGTAGTGC CACTCAGGCT 
GCATCTTTTC AACAAGGGAA CAAACAAGCT GTGCAGAGTG GTTCACTCAG TCAGTCAGCC 
AGTGAGAAGA CTTCACCTTC AGAAACAGCA CCAAGCTCAA ACAATGATGT GCAAGATAAT 
ACAAGTCCAG TGAGAAAAGA AAACGGACAG AGCCATGACC AGGACCAAAC ACCAACAACT 
AATCTGCCAC GCAAGGGCAC ATTCACAGAT GACCTCCATC AGCTGGTTGA TAACTGGGCC 
AGAGATGCCA TGAACATGAT GCCACAGGTC AAGAAAGGAT CCAAAAAGAA TGTTCATGAA 
ATGCCACGTA AATACTCCGC ACCAGGTCAG CTTTGTTCCA CTGGTGGCCA CATCCCAGCG 
ACCATCAACC CCACAGCTGG ACGGAAGGGT TCAATAGGTG TGGCCAATCA GCAATTTGGC 
TACACTTGTC CCAACTACAA CATGCCTCAA TGGAATCAGG TGGGGACAAT ATCATCCACC 
GCTCCACCTG CAGGACTACA GCAGGGTTTC CTTATCCCAT CAGGAGCCCA CCAAACCGGA 
AATAACTGCG GATCAAGCAA TCTGCGCACC ACTCAGGCAA GCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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wnk1a cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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Eメール

電話

FAX

オンラインフォーム

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