nes cDNA ORF clone, Danio rerio(zebrafish)

The following nes gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the nes cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa21583 XM_001919887.7
Latest version!
Danio rerio nestin (nes), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
View Old Accession Versions >>
ODa21583 XM_001919887.6 Danio rerio nestin (nes), mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa21583
    Clone ID Related Accession (Same CDS sequence) XM_001919887.6 , XM_001919887.7
    Accession Version XM_001919887.6 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3798bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product nestin
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001919887.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGCTTC TGGGTGTCCG ACAGCCATTT GGCCCTCAGT TTCAGGAGGA AAAGTACCAG 
    ATGCTAGAGC TGAACCACCG CCTTGAATCA TACTTGGGCC GTGTGAAACT TCTGGAGGAA
    GAGAACAAGC TACTTCGAGA GGAGATCCAC ACTCTCAAGA GCAGCAGGGA CCCAGCAGGC
    CAACGTAAAG CCCAGGAGGA GGCTCTCAGT CAGGCCAGGA GGATGATGGA GGAGGCCTGG
    AGAAAGAAAG ACTGTGTGGA GCTGGAGGTA GAGAATCTGA TGGAGGATAT GGAGAAGGTG
    AGCTTTCAAA GAAAGAAGGT GAAGACTGCA CAGGCTGAAG CTCAGAGGAA GCTCACAGAG
    AGCAGAAAGG AGCTGGAAGA GGAGCGCAGG GCTCAGATTT GGCTTAGAGA GAAGGTCGGT
    CAACTCGAGA AAGACCTCAT GCTGCAGATG CAAGTGCACC AGGAGAACAT GGAAACCATG
    CAGGCCTCAC TGAAACAGAC AAAGCAGGTC CTAATGGCTC CACAGCGTGT GCAAGCTACC
    AGCATCCCAG ACCTGGGGCA GGAGTATGGC TACAAGGCAG CTCAAGCCTG GCAGGAGGCA
    GCCAACAACT ATCAGAAACA GGTTGGACGT CTGGAGGAGA GTCTGAACCA GACAAAAGCT
    AACATGACAA AGATTTACCA GGAGAAGAGA GAGAGCCAGC GTCAGGTACA GCATCTGGCC
    AAAGAGCTGG AGAGCACGAG GATCAAGAGG CAGATGCTGG AGAAACATGC CATGCAGCAA
    AGAGAAGAAC AGAAAGAGGA GCTTCAACAT CTTCAGGCCC AAGTAAACAC CCTGGAGTTG
    GAGAAAGACT GTCTGGGGCA GCAGATTGAC AGCCTGATGT TGGATAGACA ACATCTGCTG
    CAGGTGAAGA TGTCTCTTGG ACTGGAGGTG GCAACATACA GAGCTTTGCT GGACAGCGAG
    GGCCTGAGAA TCGACAGACC AACACCAAAC AAGACCAGCT CTACTGTCTT TTTAGATGCA
    CTCTCAAAGC CCACTGGGAT CCAGTCAACT TCTCAAACCA CTGCTGCTTC CTGCCTTCTC
    AGCAGTTCTG TGTCTACAAG TCACAGATCA ATCGCCTCAT CCCGCAGTCT GCTGACCAGT
    GCGGCTCCGT CATGGACACT GACACGAGGG ACTCCACAGA GACCACCAAG CAGTACCTCG
    ATGACAGAAA AGACAGAGGA TCACATATCT GAGGAGACTA AGAGATCTGC TGAGGAGTCT
    GTGGATCAAT TACAGCAAGA GAAAGTCCAG CAAGACTGGA CTCTCGAATC CACTCTTCCC
    AAAACATCTG CAGAACCAAA ACCAGAGCTT CAACCAGAAG AAATTAAGGT GGAAGACGAA
    GCAAATGAAC CCCAACAAGC TCATATGGTT GAATCTAAAA CTGATGAATC TGAACTGACT
    AGTGTACCAG CTGAGCAACA AGGAAGTATG TCCCAAACTC CAGAGACAAA GAGCTGGGCT
    GGTCATTTCA ATGATCCTGC AGAAGTTTCA GAAGACGGAA AGGATGAAGA CACTGAAGTG
    TCTGTCGAAA TGGCTCGTAT CTCACATGCA CCCAAGGTTG CTTGGGAAGA AATCAAATCT
    GCAGTAGAGG ATGAAAAAGA TGATGCTTCT GAGATGGACG TAAGATCAGA GATCATCAGT
    GAAAGCCACA CATTTGCATA TGGAGATGCA GAGAATGATA GTAATACATT AACATCAAGC
    CATATCAGTC AAAACACCAA TGCACTGGCT TCATCTTTCC TTGAGCAAGG AACTCTAGAT
    TCAGCTAGTT ATTTTGGCTA TGAGGCAACA AATGAAAATC TTATGAAGGA AGAGGAACTG
    GACAATGTGA GCAATATTAG CGAGGAGGTG ACAGAACAAT TGAACAGTGA AACTGAGGCA
    GCTATTGATT CAATCAATGA GTGGGACAGA CAGGAAGAAA GTGAACCAGA GAATGAGACC
    AAAGTGATGA CTTCTTACTT TGAGGTAGAA GAGGGAGAAA TGAGCATCAA TACTGACACG
    AAAGATAAAA CTGAAGATGA CATAGAAAGG GAAGAGTTAG AAGTGACAGA AAGAGAAATT
    CTAGTGCAAA GTGCAGGTGG AATGCTGGGT GTAGATTTAC CCAATGACAC TGATCATCAG
    GATGAGCACA ATCTACCTCA GATAGAGCTG AACGAAGACC AATCTGAGGC AGAAGAAGAA
    AATCAAAAAG AAAAGCAATG TGATGAAGAT GACTCCCCAA ACATATCAGC CTCATTGAAA
    ACTGACCCAG GAGAAGGAGA CAGTTACAGC CAGGAGCACA CGCTGGCAGA CACACGGCCC
    TTGATCCGTT ATAAGAGCGA TGAGGAAACC TCACACCACA TCGGTGAGAC CAGTGACAGT
    GAGGATGAGA AAGAAAGAAT TGATCAAGGC CACTTGAATG AGAAACGTTT TAACACTATG
    GAGGATCTCA CTGAAGAACC AGACATGGAG GTCACCGGAA AGATGAGGCT GGAGGATCTT
    GTTTCTAATG AAGAAGCTGC AGCTGACGAT GTGGCATCTG TGATGTTTCA GAAAGTCTCT
    GGAGACCATG AAAGTTTGGA TGTGGTGGTG CAAGAAAGTG AACGCAAGGG TAATCTGGAG
    AAGGAAGATA GTGGTGATGC TGATAATATG AGAGACAATA AGGAAGAAGA TGTCAGTGTT
    TTTGAGCAAA ATGAGAACCA GCAACTGACT GAACACCAGC ATGTTCACAC AGAACAGGTT
    GAGGATAAAC CAGTCCATTC ACATGAAACT CAAGAACACG TCAACACGGA GTTTTCATCC
    ACATTTTCTG AAAGATCTCA GCAAGAGCAA CTGGAAGAAT CAAGTCTGAC TATGTTTGAA
    GATGAAGCTG CAACCAAAGA AGCAGAAGAT TCGGATGTCT CTATGCACAC CAACATAGAT
    CTGATTGACA GTCGCTCTTT AGAGAGCGAG ATAAACGGTC AGCCAGACAT TATGACCTCA
    GACATGGCCA ACTCTGAGTG CAATAGCTCA GAAGACGAGT CTCCAAATGC CAGCCAGTGC
    TTTCAGAACA CAAGCCTTTT AGCAGCGGCG ACTCCTAATG AACAGCCATT AACCTTTACT
    AATGAAGTCT CCAAGGCTGA TTATGTGTCT GACAACAACG ACGTGCCTGA AGAAGTGCTC
    AGTGAGGAGA AGAGCACTGA TGAGCCCAAG GCCTCTCAGA TTGATGACTT GGAGAATTTT
    AACATTTGGG GGGAAAGCAC GAGCATTTCA GATGCAGCCC AGACCACACA TGCAAGCATG
    GATGAACATT CAAGCATTTC CCCAGTGAAT GAGAATCTGG AAAGCTCAAA CAAAATACCA
    AGTGTGGCGG AAAACATCTT TGGACATTTT GAAGAACATC TCGAAAGGTC AGTGGAGAGC
    TTTCCAGAAA AAGAAGTGAC TGTGATGGAC TTTGAGAACA ACGATTTAGG AGAGGAATTT
    CAGTCGAAGC AAATGAAAAG TGAGATTCAC AGCTTCTTCA GCACTAGTTT GAAGCAAGAT
    TTCTGGAGCG AAGGGAAAAT GGAGATGGCA GCTACTTATG ATCCCGCCAA AACTGAAGAC
    TTAAATCAGG CCATGGTCTT TGGAGAGGAG TGGAGAGAAA TAGGAGTGCA ATCAGCGAAT
    GGAGATACTA AAGAAGAGAT GGAGATCCTC AAAATCAGGG ATGATAAGCA AAAAGATGGA
    CAGCCAGCGC AGAGCAAGAT AGTCCAGTCA GATGATTCTG CTGATGAGGG GGATTCCTGG
    TCATCTGGTG ATGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001919922.1
    CDS142..3939
    Translation

    Target ORF information:

    RefSeq Version XM_001919887.6
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio nestin (nes), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001919887.6

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGCTTC TGGGTGTCCG ACAGCCATTT GGCCCTCAGT TTCAGGAGGA AAAGTACCAG 
    ATGCTAGAGC TGAACCACCG CCTTGAATCA TACTTGGGCC GTGTGAAACT TCTGGAGGAA
    GAGAACAAGC TACTTCGAGA GGAGATCCAC ACTCTCAAGA GCAGCAGGGA CCCAGCAGGC
    CAACGTAAAG CCCAGGAGGA GGCTCTCAGT CAGGCCAGGA GGATGATGGA GGAGGCCTGG
    AGAAAGAAAG ACTGTGTGGA GCTGGAGGTA GAGAATCTGA TGGAGGATAT GGAGAAGGTG
    AGCTTTCAAA GAAAGAAGGT GAAGACTGCA CAGGCTGAAG CTCAGAGGAA GCTCACAGAG
    AGCAGAAAGG AGCTGGAAGA GGAGCGCAGG GCTCAGATTT GGCTTAGAGA GAAGGTCGGT
    CAACTCGAGA AAGACCTCAT GCTGCAGATG CAAGTGCACC AGGAGAACAT GGAAACCATG
    CAGGCCTCAC TGAAACAGAC AAAGCAGGTC CTAATGGCTC CACAGCGTGT GCAAGCTACC
    AGCATCCCAG ACCTGGGGCA GGAGTATGGC TACAAGGCAG CTCAAGCCTG GCAGGAGGCA
    GCCAACAACT ATCAGAAACA GGTTGGACGT CTGGAGGAGA GTCTGAACCA GACAAAAGCT
    AACATGACAA AGATTTACCA GGAGAAGAGA GAGAGCCAGC GTCAGGTACA GCATCTGGCC
    AAAGAGCTGG AGAGCACGAG GATCAAGAGG CAGATGCTGG AGAAACATGC CATGCAGCAA
    AGAGAAGAAC AGAAAGAGGA GCTTCAACAT CTTCAGGCCC AAGTAAACAC CCTGGAGTTG
    GAGAAAGACT GTCTGGGGCA GCAGATTGAC AGCCTGATGT TGGATAGACA ACATCTGCTG
    CAGGTGAAGA TGTCTCTTGG ACTGGAGGTG GCAACATACA GAGCTTTGCT GGACAGCGAG
    GGCCTGAGAA TCGACAGACC AACACCAAAC AAGACCAGCT CTACTGTCTT TTTAGATGCA
    CTCTCAAAGC CCACTGGGAT CCAGTCAACT TCTCAAACCA CTGCTGCTTC CTGCCTTCTC
    AGCAGTTCTG TGTCTACAAG TCACAGATCA ATCGCCTCAT CCCGCAGTCT GCTGACCAGT
    GCGGCTCCGT CATGGACACT GACACGAGGG ACTCCACAGA GACCACCAAG CAGTACCTCG
    ATGACAGAAA AGACAGAGGA TCACATATCT GAGGAGACTA AGAGATCTGC TGAGGAGTCT
    GTGGATCAAT TACAGCAAGA GAAAGTCCAG CAAGACTGGA CTCTCGAATC CACTCTTCCC
    AAAACATCTG CAGAACCAAA ACCAGAGCTT CAACCAGAAG AAATTAAGGT GGAAGACGAA
    GCAAATGAAC CCCAACAAGC TCATATGGTT GAATCTAAAA CTGATGAATC TGAACTGACT
    AGTGTACCAG CTGAGCAACA AGGAAGTATG TCCCAAACTC CAGAGACAAA GAGCTGGGCT
    GGTCATTTCA ATGATCCTGC AGAAGTTTCA GAAGACGGAA AGGATGAAGA CACTGAAGTG
    TCTGTCGAAA TGGCTCGTAT CTCACATGCA CCCAAGGTTG CTTGGGAAGA AATCAAATCT
    GCAGTAGAGG ATGAAAAAGA TGATGCTTCT GAGATGGACG TAAGATCAGA GATCATCAGT
    GAAAGCCACA CATTTGCATA TGGAGATGCA GAGAATGATA GTAATACATT AACATCAAGC
    CATATCAGTC AAAACACCAA TGCACTGGCT TCATCTTTCC TTGAGCAAGG AACTCTAGAT
    TCAGCTAGTT ATTTTGGCTA TGAGGCAACA AATGAAAATC TTATGAAGGA AGAGGAACTG
    GACAATGTGA GCAATATTAG CGAGGAGGTG ACAGAACAAT TGAACAGTGA AACTGAGGCA
    GCTATTGATT CAATCAATGA GTGGGACAGA CAGGAAGAAA GTGAACCAGA GAATGAGACC
    AAAGTGATGA CTTCTTACTT TGAGGTAGAA GAGGGAGAAA TGAGCATCAA TACTGACACG
    AAAGATAAAA CTGAAGATGA CATAGAAAGG GAAGAGTTAG AAGTGACAGA AAGAGAAATT
    CTAGTGCAAA GTGCAGGTGG AATGCTGGGT GTAGATTTAC CCAATGACAC TGATCATCAG
    GATGAGCACA ATCTACCTCA GATAGAGCTG AACGAAGACC AATCTGAGGC AGAAGAAGAA
    AATCAAAAAG AAAAGCAATG TGATGAAGAT GACTCCCCAA ACATATCAGC CTCATTGAAA
    ACTGACCCAG GAGAAGGAGA CAGTTACAGC CAGGAGCACA CGCTGGCAGA CACACGGCCC
    TTGATCCGTT ATAAGAGCGA TGAGGAAACC TCACACCACA TCGGTGAGAC CAGTGACAGT
    GAGGATGAGA AAGAAAGAAT TGATCAAGGC CACTTGAATG AGAAACGTTT TAACACTATG
    GAGGATCTCA CTGAAGAACC AGACATGGAG GTCACCGGAA AGATGAGGCT GGAGGATCTT
    GTTTCTAATG AAGAAGCTGC AGCTGACGAT GTGGCATCTG TGATGTTTCA GAAAGTCTCT
    GGAGACCATG AAAGTTTGGA TGTGGTGGTG CAAGAAAGTG AACGCAAGGG TAATCTGGAG
    AAGGAAGATA GTGGTGATGC TGATAATATG AGAGACAATA AGGAAGAAGA TGTCAGTGTT
    TTTGAGCAAA ATGAGAACCA GCAACTGACT GAACACCAGC ATGTTCACAC AGAACAGGTT
    GAGGATAAAC CAGTCCATTC ACATGAAACT CAAGAACACG TCAACACGGA GTTTTCATCC
    ACATTTTCTG AAAGATCTCA GCAAGAGCAA CTGGAAGAAT CAAGTCTGAC TATGTTTGAA
    GATGAAGCTG CAACCAAAGA AGCAGAAGAT TCGGATGTCT CTATGCACAC CAACATAGAT
    CTGATTGACA GTCGCTCTTT AGAGAGCGAG ATAAACGGTC AGCCAGACAT TATGACCTCA
    GACATGGCCA ACTCTGAGTG CAATAGCTCA GAAGACGAGT CTCCAAATGC CAGCCAGTGC
    TTTCAGAACA CAAGCCTTTT AGCAGCGGCG ACTCCTAATG AACAGCCATT AACCTTTACT
    AATGAAGTCT CCAAGGCTGA TTATGTGTCT GACAACAACG ACGTGCCTGA AGAAGTGCTC
    AGTGAGGAGA AGAGCACTGA TGAGCCCAAG GCCTCTCAGA TTGATGACTT GGAGAATTTT
    AACATTTGGG GGGAAAGCAC GAGCATTTCA GATGCAGCCC AGACCACACA TGCAAGCATG
    GATGAACATT CAAGCATTTC CCCAGTGAAT GAGAATCTGG AAAGCTCAAA CAAAATACCA
    AGTGTGGCGG AAAACATCTT TGGACATTTT GAAGAACATC TCGAAAGGTC AGTGGAGAGC
    TTTCCAGAAA AAGAAGTGAC TGTGATGGAC TTTGAGAACA ACGATTTAGG AGAGGAATTT
    CAGTCGAAGC AAATGAAAAG TGAGATTCAC AGCTTCTTCA GCACTAGTTT GAAGCAAGAT
    TTCTGGAGCG AAGGGAAAAT GGAGATGGCA GCTACTTATG ATCCCGCCAA AACTGAAGAC
    TTAAATCAGG CCATGGTCTT TGGAGAGGAG TGGAGAGAAA TAGGAGTGCA ATCAGCGAAT
    GGAGATACTA AAGAAGAGAT GGAGATCCTC AAAATCAGGG ATGATAAGCA AAAAGATGGA
    CAGCCAGCGC AGAGCAAGAT AGTCCAGTCA GATGATTCTG CTGATGAGGG GGATTCCTGG
    TCATCTGGTG ATGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa21583
    Clone ID Related Accession (Same CDS sequence) XM_001919887.6 , XM_001919887.7
    Accession Version XM_001919887.7 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3798bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product nestin
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007127.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001919887.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGCTTC TGGGTGTCCG ACAGCCATTT GGCCCTCAGT TTCAGGAGGA AAAGTACCAG 
    ATGCTAGAGC TGAACCACCG CCTTGAATCA TACTTGGGCC GTGTGAAACT TCTGGAGGAA
    GAGAACAAGC TACTTCGAGA GGAGATCCAC ACTCTCAAGA GCAGCAGGGA CCCAGCAGGC
    CAACGTAAAG CCCAGGAGGA GGCTCTCAGT CAGGCCAGGA GGATGATGGA GGAGGCCTGG
    AGAAAGAAAG ACTGTGTGGA GCTGGAGGTA GAGAATCTGA TGGAGGATAT GGAGAAGGTG
    AGCTTTCAAA GAAAGAAGGT GAAGACTGCA CAGGCTGAAG CTCAGAGGAA GCTCACAGAG
    AGCAGAAAGG AGCTGGAAGA GGAGCGCAGG GCTCAGATTT GGCTTAGAGA GAAGGTCGGT
    CAACTCGAGA AAGACCTCAT GCTGCAGATG CAAGTGCACC AGGAGAACAT GGAAACCATG
    CAGGCCTCAC TGAAACAGAC AAAGCAGGTC CTAATGGCTC CACAGCGTGT GCAAGCTACC
    AGCATCCCAG ACCTGGGGCA GGAGTATGGC TACAAGGCAG CTCAAGCCTG GCAGGAGGCA
    GCCAACAACT ATCAGAAACA GGTTGGACGT CTGGAGGAGA GTCTGAACCA GACAAAAGCT
    AACATGACAA AGATTTACCA GGAGAAGAGA GAGAGCCAGC GTCAGGTACA GCATCTGGCC
    AAAGAGCTGG AGAGCACGAG GATCAAGAGG CAGATGCTGG AGAAACATGC CATGCAGCAA
    AGAGAAGAAC AGAAAGAGGA GCTTCAACAT CTTCAGGCCC AAGTAAACAC CCTGGAGTTG
    GAGAAAGACT GTCTGGGGCA GCAGATTGAC AGCCTGATGT TGGATAGACA ACATCTGCTG
    CAGGTGAAGA TGTCTCTTGG ACTGGAGGTG GCAACATACA GAGCTTTGCT GGACAGCGAG
    GGCCTGAGAA TCGACAGACC AACACCAAAC AAGACCAGCT CTACTGTCTT TTTAGATGCA
    CTCTCAAAGC CCACTGGGAT CCAGTCAACT TCTCAAACCA CTGCTGCTTC CTGCCTTCTC
    AGCAGTTCTG TGTCTACAAG TCACAGATCA ATCGCCTCAT CCCGCAGTCT GCTGACCAGT
    GCGGCTCCGT CATGGACACT GACACGAGGG ACTCCACAGA GACCACCAAG CAGTACCTCG
    ATGACAGAAA AGACAGAGGA TCACATATCT GAGGAGACTA AGAGATCTGC TGAGGAGTCT
    GTGGATCAAT TACAGCAAGA GAAAGTCCAG CAAGACTGGA CTCTCGAATC CACTCTTCCC
    AAAACATCTG CAGAACCAAA ACCAGAGCTT CAACCAGAAG AAATTAAGGT GGAAGACGAA
    GCAAATGAAC CCCAACAAGC TCATATGGTT GAATCTAAAA CTGATGAATC TGAACTGACT
    AGTGTACCAG CTGAGCAACA AGGAAGTATG TCCCAAACTC CAGAGACAAA GAGCTGGGCT
    GGTCATTTCA ATGATCCTGC AGAAGTTTCA GAAGACGGAA AGGATGAAGA CACTGAAGTG
    TCTGTCGAAA TGGCTCGTAT CTCACATGCA CCCAAGGTTG CTTGGGAAGA AATCAAATCT
    GCAGTAGAGG ATGAAAAAGA TGATGCTTCT GAGATGGACG TAAGATCAGA GATCATCAGT
    GAAAGCCACA CATTTGCATA TGGAGATGCA GAGAATGATA GTAATACATT AACATCAAGC
    CATATCAGTC AAAACACCAA TGCACTGGCT TCATCTTTCC TTGAGCAAGG AACTCTAGAT
    TCAGCTAGTT ATTTTGGCTA TGAGGCAACA AATGAAAATC TTATGAAGGA AGAGGAACTG
    GACAATGTGA GCAATATTAG CGAGGAGGTG ACAGAACAAT TGAACAGTGA AACTGAGGCA
    GCTATTGATT CAATCAATGA GTGGGACAGA CAGGAAGAAA GTGAACCAGA GAATGAGACC
    AAAGTGATGA CTTCTTACTT TGAGGTAGAA GAGGGAGAAA TGAGCATCAA TACTGACACG
    AAAGATAAAA CTGAAGATGA CATAGAAAGG GAAGAGTTAG AAGTGACAGA AAGAGAAATT
    CTAGTGCAAA GTGCAGGTGG AATGCTGGGT GTAGATTTAC CCAATGACAC TGATCATCAG
    GATGAGCACA ATCTACCTCA GATAGAGCTG AACGAAGACC AATCTGAGGC AGAAGAAGAA
    AATCAAAAAG AAAAGCAATG TGATGAAGAT GACTCCCCAA ACATATCAGC CTCATTGAAA
    ACTGACCCAG GAGAAGGAGA CAGTTACAGC CAGGAGCACA CGCTGGCAGA CACACGGCCC
    TTGATCCGTT ATAAGAGCGA TGAGGAAACC TCACACCACA TCGGTGAGAC CAGTGACAGT
    GAGGATGAGA AAGAAAGAAT TGATCAAGGC CACTTGAATG AGAAACGTTT TAACACTATG
    GAGGATCTCA CTGAAGAACC AGACATGGAG GTCACCGGAA AGATGAGGCT GGAGGATCTT
    GTTTCTAATG AAGAAGCTGC AGCTGACGAT GTGGCATCTG TGATGTTTCA GAAAGTCTCT
    GGAGACCATG AAAGTTTGGA TGTGGTGGTG CAAGAAAGTG AACGCAAGGG TAATCTGGAG
    AAGGAAGATA GTGGTGATGC TGATAATATG AGAGACAATA AGGAAGAAGA TGTCAGTGTT
    TTTGAGCAAA ATGAGAACCA GCAACTGACT GAACACCAGC ATGTTCACAC AGAACAGGTT
    GAGGATAAAC CAGTCCATTC ACATGAAACT CAAGAACACG TCAACACGGA GTTTTCATCC
    ACATTTTCTG AAAGATCTCA GCAAGAGCAA CTGGAAGAAT CAAGTCTGAC TATGTTTGAA
    GATGAAGCTG CAACCAAAGA AGCAGAAGAT TCGGATGTCT CTATGCACAC CAACATAGAT
    CTGATTGACA GTCGCTCTTT AGAGAGCGAG ATAAACGGTC AGCCAGACAT TATGACCTCA
    GACATGGCCA ACTCTGAGTG CAATAGCTCA GAAGACGAGT CTCCAAATGC CAGCCAGTGC
    TTTCAGAACA CAAGCCTTTT AGCAGCGGCG ACTCCTAATG AACAGCCATT AACCTTTACT
    AATGAAGTCT CCAAGGCTGA TTATGTGTCT GACAACAACG ACGTGCCTGA AGAAGTGCTC
    AGTGAGGAGA AGAGCACTGA TGAGCCCAAG GCCTCTCAGA TTGATGACTT GGAGAATTTT
    AACATTTGGG GGGAAAGCAC GAGCATTTCA GATGCAGCCC AGACCACACA TGCAAGCATG
    GATGAACATT CAAGCATTTC CCCAGTGAAT GAGAATCTGG AAAGCTCAAA CAAAATACCA
    AGTGTGGCGG AAAACATCTT TGGACATTTT GAAGAACATC TCGAAAGGTC AGTGGAGAGC
    TTTCCAGAAA AAGAAGTGAC TGTGATGGAC TTTGAGAACA ACGATTTAGG AGAGGAATTT
    CAGTCGAAGC AAATGAAAAG TGAGATTCAC AGCTTCTTCA GCACTAGTTT GAAGCAAGAT
    TTCTGGAGCG AAGGGAAAAT GGAGATGGCA GCTACTTATG ATCCCGCCAA AACTGAAGAC
    TTAAATCAGG CCATGGTCTT TGGAGAGGAG TGGAGAGAAA TAGGAGTGCA ATCAGCGAAT
    GGAGATACTA AAGAAGAGAT GGAGATCCTC AAAATCAGGG ATGATAAGCA AAAAGATGGA
    CAGCCAGCGC AGAGCAAGAT AGTCCAGTCA GATGATTCTG CTGATGAGGG GGATTCCTGG
    TCATCTGGTG ATGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_001919922.1
    CDS183..3980
    Translation

    Target ORF information:

    RefSeq Version XM_001919887.7
    Organism Danio rerio(zebrafish)
    Definition Danio rerio nestin (nes), mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_001919887.7

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    ATGGAGCTTC TGGGTGTCCG ACAGCCATTT GGCCCTCAGT TTCAGGAGGA AAAGTACCAG 
    ATGCTAGAGC TGAACCACCG CCTTGAATCA TACTTGGGCC GTGTGAAACT TCTGGAGGAA
    GAGAACAAGC TACTTCGAGA GGAGATCCAC ACTCTCAAGA GCAGCAGGGA CCCAGCAGGC
    CAACGTAAAG CCCAGGAGGA GGCTCTCAGT CAGGCCAGGA GGATGATGGA GGAGGCCTGG
    AGAAAGAAAG ACTGTGTGGA GCTGGAGGTA GAGAATCTGA TGGAGGATAT GGAGAAGGTG
    AGCTTTCAAA GAAAGAAGGT GAAGACTGCA CAGGCTGAAG CTCAGAGGAA GCTCACAGAG
    AGCAGAAAGG AGCTGGAAGA GGAGCGCAGG GCTCAGATTT GGCTTAGAGA GAAGGTCGGT
    CAACTCGAGA AAGACCTCAT GCTGCAGATG CAAGTGCACC AGGAGAACAT GGAAACCATG
    CAGGCCTCAC TGAAACAGAC AAAGCAGGTC CTAATGGCTC CACAGCGTGT GCAAGCTACC
    AGCATCCCAG ACCTGGGGCA GGAGTATGGC TACAAGGCAG CTCAAGCCTG GCAGGAGGCA
    GCCAACAACT ATCAGAAACA GGTTGGACGT CTGGAGGAGA GTCTGAACCA GACAAAAGCT
    AACATGACAA AGATTTACCA GGAGAAGAGA GAGAGCCAGC GTCAGGTACA GCATCTGGCC
    AAAGAGCTGG AGAGCACGAG GATCAAGAGG CAGATGCTGG AGAAACATGC CATGCAGCAA
    AGAGAAGAAC AGAAAGAGGA GCTTCAACAT CTTCAGGCCC AAGTAAACAC CCTGGAGTTG
    GAGAAAGACT GTCTGGGGCA GCAGATTGAC AGCCTGATGT TGGATAGACA ACATCTGCTG
    CAGGTGAAGA TGTCTCTTGG ACTGGAGGTG GCAACATACA GAGCTTTGCT GGACAGCGAG
    GGCCTGAGAA TCGACAGACC AACACCAAAC AAGACCAGCT CTACTGTCTT TTTAGATGCA
    CTCTCAAAGC CCACTGGGAT CCAGTCAACT TCTCAAACCA CTGCTGCTTC CTGCCTTCTC
    AGCAGTTCTG TGTCTACAAG TCACAGATCA ATCGCCTCAT CCCGCAGTCT GCTGACCAGT
    GCGGCTCCGT CATGGACACT GACACGAGGG ACTCCACAGA GACCACCAAG CAGTACCTCG
    ATGACAGAAA AGACAGAGGA TCACATATCT GAGGAGACTA AGAGATCTGC TGAGGAGTCT
    GTGGATCAAT TACAGCAAGA GAAAGTCCAG CAAGACTGGA CTCTCGAATC CACTCTTCCC
    AAAACATCTG CAGAACCAAA ACCAGAGCTT CAACCAGAAG AAATTAAGGT GGAAGACGAA
    GCAAATGAAC CCCAACAAGC TCATATGGTT GAATCTAAAA CTGATGAATC TGAACTGACT
    AGTGTACCAG CTGAGCAACA AGGAAGTATG TCCCAAACTC CAGAGACAAA GAGCTGGGCT
    GGTCATTTCA ATGATCCTGC AGAAGTTTCA GAAGACGGAA AGGATGAAGA CACTGAAGTG
    TCTGTCGAAA TGGCTCGTAT CTCACATGCA CCCAAGGTTG CTTGGGAAGA AATCAAATCT
    GCAGTAGAGG ATGAAAAAGA TGATGCTTCT GAGATGGACG TAAGATCAGA GATCATCAGT
    GAAAGCCACA CATTTGCATA TGGAGATGCA GAGAATGATA GTAATACATT AACATCAAGC
    CATATCAGTC AAAACACCAA TGCACTGGCT TCATCTTTCC TTGAGCAAGG AACTCTAGAT
    TCAGCTAGTT ATTTTGGCTA TGAGGCAACA AATGAAAATC TTATGAAGGA AGAGGAACTG
    GACAATGTGA GCAATATTAG CGAGGAGGTG ACAGAACAAT TGAACAGTGA AACTGAGGCA
    GCTATTGATT CAATCAATGA GTGGGACAGA CAGGAAGAAA GTGAACCAGA GAATGAGACC
    AAAGTGATGA CTTCTTACTT TGAGGTAGAA GAGGGAGAAA TGAGCATCAA TACTGACACG
    AAAGATAAAA CTGAAGATGA CATAGAAAGG GAAGAGTTAG AAGTGACAGA AAGAGAAATT
    CTAGTGCAAA GTGCAGGTGG AATGCTGGGT GTAGATTTAC CCAATGACAC TGATCATCAG
    GATGAGCACA ATCTACCTCA GATAGAGCTG AACGAAGACC AATCTGAGGC AGAAGAAGAA
    AATCAAAAAG AAAAGCAATG TGATGAAGAT GACTCCCCAA ACATATCAGC CTCATTGAAA
    ACTGACCCAG GAGAAGGAGA CAGTTACAGC CAGGAGCACA CGCTGGCAGA CACACGGCCC
    TTGATCCGTT ATAAGAGCGA TGAGGAAACC TCACACCACA TCGGTGAGAC CAGTGACAGT
    GAGGATGAGA AAGAAAGAAT TGATCAAGGC CACTTGAATG AGAAACGTTT TAACACTATG
    GAGGATCTCA CTGAAGAACC AGACATGGAG GTCACCGGAA AGATGAGGCT GGAGGATCTT
    GTTTCTAATG AAGAAGCTGC AGCTGACGAT GTGGCATCTG TGATGTTTCA GAAAGTCTCT
    GGAGACCATG AAAGTTTGGA TGTGGTGGTG CAAGAAAGTG AACGCAAGGG TAATCTGGAG
    AAGGAAGATA GTGGTGATGC TGATAATATG AGAGACAATA AGGAAGAAGA TGTCAGTGTT
    TTTGAGCAAA ATGAGAACCA GCAACTGACT GAACACCAGC ATGTTCACAC AGAACAGGTT
    GAGGATAAAC CAGTCCATTC ACATGAAACT CAAGAACACG TCAACACGGA GTTTTCATCC
    ACATTTTCTG AAAGATCTCA GCAAGAGCAA CTGGAAGAAT CAAGTCTGAC TATGTTTGAA
    GATGAAGCTG CAACCAAAGA AGCAGAAGAT TCGGATGTCT CTATGCACAC CAACATAGAT
    CTGATTGACA GTCGCTCTTT AGAGAGCGAG ATAAACGGTC AGCCAGACAT TATGACCTCA
    GACATGGCCA ACTCTGAGTG CAATAGCTCA GAAGACGAGT CTCCAAATGC CAGCCAGTGC
    TTTCAGAACA CAAGCCTTTT AGCAGCGGCG ACTCCTAATG AACAGCCATT AACCTTTACT
    AATGAAGTCT CCAAGGCTGA TTATGTGTCT GACAACAACG ACGTGCCTGA AGAAGTGCTC
    AGTGAGGAGA AGAGCACTGA TGAGCCCAAG GCCTCTCAGA TTGATGACTT GGAGAATTTT
    AACATTTGGG GGGAAAGCAC GAGCATTTCA GATGCAGCCC AGACCACACA TGCAAGCATG
    GATGAACATT CAAGCATTTC CCCAGTGAAT GAGAATCTGG AAAGCTCAAA CAAAATACCA
    AGTGTGGCGG AAAACATCTT TGGACATTTT GAAGAACATC TCGAAAGGTC AGTGGAGAGC
    TTTCCAGAAA AAGAAGTGAC TGTGATGGAC TTTGAGAACA ACGATTTAGG AGAGGAATTT
    CAGTCGAAGC AAATGAAAAG TGAGATTCAC AGCTTCTTCA GCACTAGTTT GAAGCAAGAT
    TTCTGGAGCG AAGGGAAAAT GGAGATGGCA GCTACTTATG ATCCCGCCAA AACTGAAGAC
    TTAAATCAGG CCATGGTCTT TGGAGAGGAG TGGAGAGAAA TAGGAGTGCA ATCAGCGAAT
    GGAGATACTA AAGAAGAGAT GGAGATCCTC AAAATCAGGG ATGATAAGCA AAAAGATGGA
    CAGCCAGCGC AGAGCAAGAT AGTCCAGTCA GATGATTCTG CTGATGAGGG GGATTCCTGG
    TCATCTGGTG ATGAGTAA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.