kalrna gene cDNA ORF clone, Danio rerio(zebrafish)

mRNA	Protein	Name
XM_021478826.1	XP_021334501.1	kalirin isoform X3
XM_009304451.3	XP_009302726.2	kalirin isoform X4
XM_009304455.2	XP_009302730.1	kalirin isoform X5
XM_017357741.2	XP_017213230.1	kalirin isoform X1
XM_009304455.3	XP_009302730.1	kalirin isoform X6
XM_009304450.2	XP_009302725.2	kalirin isoform X2
XM_009304454.3	XP_009302729.1	kalirin isoform X5
XM_009304451.2	XP_009302726.2	kalirin isoform X3
XM_009304454.2	XP_009302729.1	kalirin isoform X4
XM_017357741.1	XP_017213230.1	kalirin isoform X1
XM_009304450.3	XP_009302725.2	kalirin isoform X2

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa48698	XM_009304454.3 Latest version!	Danio rerio kalirin RhoGEF kinase a (kalrna), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa48699	XM_009304455.3 Latest version!	Danio rerio kalirin RhoGEF kinase a (kalrna), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa144040	XM_021478826.1 Latest version!	Danio rerio kalirin RhoGEF kinase a (kalrna), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa60287	XM_017357741.2 Latest version!	Danio rerio kalirin RhoGEF kinase a (kalrna), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa60288	XM_009304450.3 Latest version!	Danio rerio kalirin RhoGEF kinase a (kalrna), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa60289	XM_009304451.3 Latest version!	Danio rerio kalirin RhoGEF kinase a (kalrna), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
View Old Accession Versions >>
ODa48699	XM_009304455.2	Danio rerio kalirin, RhoGEF kinase a (kalrna), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$643.30 $919.00
ODa60289	XM_009304451.2	Danio rerio kalirin, RhoGEF kinase a (kalrna), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa48698	XM_009304454.2	Danio rerio kalirin, RhoGEF kinase a (kalrna), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$727.30 $1039.00
ODa60287	XM_017357741.1	Danio rerio kalirin, RhoGEF kinase a (kalrna), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
ODa60288	XM_009304450.2	Danio rerio kalirin, RhoGEF kinase a (kalrna), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	TBD	Quote Price
Hide Old Accession Versions >>

kalrna ( XM_009304454.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302729.1 Danio rerio kalirin RhoGEF kinase a (kalrna), transcript variant X5, mRNA.

Price & Availability↑

CloneID	ODa48698
Clone ID Related Accession (Same CDS sequence)	XM_009304454.3 , XM_009304454.2
Accession Version	XM_009304454.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kalirin isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304454.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAAGT GGATGAGTTT TAAACTTTTC AAAGAGAGCA GTCGTCTCTC TGGAGGGTGT 
GAGCTGACGG TGGTCCTGCA GGACTTTACA GCAAGCAGCT CATCAGAGCT CAGCATCCAG 
ACGGGTCAGA CAGTGGAGCT GCTGGAGCGG CCCAGTGACC GTCCAGGATG GTGTCTGGTA 
CGGACCACCG AAAAGACGCC TCCTCAAGAG GGACTGGTGC CCAGCAGTGC TCTGTGTATC 
TCGCACTCAC GCAGCAGTGT GGAGATGGAC TGCTTTTTCC CAGCAGGCAA AGAAAACTAT 
ACAGTTAGTG GCCCAGAAGG AAAGACAGAG TCAGTGGCCA CACTGCAGCC ACAGCCATCC 
CTGAACTCTC TGCAGAGTAG CTCTCCTGGA CCCAAGCGCT CTGGGAATAC ACTGAAGAAA 
TGGCTGACCA GTCCTGTCCG ACGTCTAAGT CATGGCAGTA ACATCAAGAA GATCCCAAGT 
AAACAGAAAC AGAAACGTGA TGGTCGCAAG AGTATTGATA TGGGGCCGCC CGAACTACAG 
GATGACACCT TGGAAGAGCG AGTGCAAAAA GAAGAGGGCA CACTCTCCAA GTCTTCGTCT 
GGGATGCACA GTGGAGGAGA GGAAGAGCCT GAGGACGAGT CTCACACTCC TCTACCTCCA 
CCAATGGAGA TCATTAAAGA CCCGTCGGCT CAGGAAGAGA AGTCGTCTGT GCTTCTGGCT 
TCCCGGCAGA GCTCGGCTGA TGTCCCCAGT GCTGCTGAGC TTGTCAGTGC TATAGAAAAA 
CTGGTCAAAA CTAAAATGAC ACTGGAGCCA GGCTCATACC CGGGATTCAG CTCAGTCAAT 
CCAGCAGAGC AGAGCCCGAT TCCACCCAGA AACCCAGAAC TAGAGCAGAA AGCCAAAGCG 
CTACGAGGAC GAATGTTTGT AGTCAAGGAA TTAATCCAAA CGGAAAAAGA CTATGTTAAA 
GATCTGGGAA TTGTGGTTGA GGGGTTTATG AAGAGAATCG AGGAGAAAGG AGTTCCTGAT 
AACATGAAAG GAAAAGATAA GATCGTCTTC GGAAACATTC ACCAAATCTA TGACTGGCAC 
AAAGATTTTT TTGTTGGAGA ACTGGAGAAA TGCCTGGAAG ATCACGAAAA ACTGCCTGAA 
CTGTTCTGTA AACATGAGAG ACGGTTACAC ATGTATGTAG TTTACTGTCA GAACAAACCC 
AAATCCGAGT TCATCGTTGC AGAATATGAT TCATACTTTG AGGGGATTCA GCAAGACATT 
AACTCCAGAC TGAGCATCAG CGATTTTCTC ATTAAACCCA TTCAGAGAAT TACTAAATAC 
CAGCTTTTAT TAAAGGATGT TTTGAAGTAC AGTACAAAAG CAGGACTGGA TTGTCAGGAT 
ATAGAGAATG CAGTTGACCT GATGTCCCAG GTTCCCAAGC TTTGCAATGA CATGATGAAT 
CTGGGAAGAC TGCAGGGATA TGAGGGGAAG TTGACGAGTC AAGGAAAACT CTTGCAGCAG 
GAGACGTTCT TTGTGACCGA GCAGGACTCT GGCGTTCTGT CCCGCAGTAA AGAGCGCAGG 
GTTTTCCTGT TCGAACAAAT TGTCATCTTC AGCGAGCTGT TACGTAAAGG CTCTTCCACT 
CCAGGGTACC AGTTCAAGAA GAGCATAAAG GTGAGCTTCC TTGACTTGGA AGAGCATGTT 
GAAAATGATC CATGCAAGTT TGTGCTGTCG TGTCGTGGGT CGGCAGAACG TTTTACCCTT 
CAAGCAGCAA ACACGGACAT TAAACAAGTC TGGGTTCAGC ATATCACCCA ACTACTAGAT 
TTACAAAGCA ACTTCTTGTC AGCTCTGCAG TCTCCTATTG AGTATCAGAA GGAGCGTAGT 
GGCTCTCAAT CTCTGACCCG GAACTCTTCT GGGCGGATGG GTCAGTCCAA CAGCAGGCCC 
TGCTCCACGG TTTCACTCGG AGCAGAGAAA CCCTCTCTGT CTGGACGGAG CTCCACCCCG 
CTAAAGCTAT CTACCTCCAA TGGTGGTCCA TGCTCTGAAA GATACCGCAG GCAGTTTGAA 
GGCTTGGGCT GCAACGGTAC ATCGTCCTCC TTGATGGTGA CTCAAGATTA TACTGCACTG 
AAAGAGAATG AGATCTGTGT CACACAGGGT GAGGCCGTGC AGGTACTGGC CACTAACCAG 
CAGAACATGT ACCTTGTGTA CCGACCCGCA AATAGCCAAT CACCACCTGC AGAGGGCTGG 
GTGCCGGGAC ACATCCTAGG CCCCGTCACA AAACCTCTCA TAGACACTAC TGCAGACGCA 
AGCATCAAGA AATCTCTTTC GTGGAACACA CTTCGTGCTC GTAAGCGTGC AGAAAAGGAC 
AGCACGCCCC TCAAAAGTGA CTTTAAAGTT GAGAATGGAC TGCGTAAGCC AAAGGAAATT 
CTCAGCAGCA AAGTCAAAGT GAAAGAGTCC CGCAGTTCTG ATGAGTCTGA ATGTGAGGAT 
GAACTAGACC CCAAAACTAG TATGGAGTTA CTGAACCCTA ATTTCATCCA AGAGGTCGCT 
CCAGAGTTTC TGACGCCTCT AACAGACGTG ACGTGTGCTC TGGGAGAGAC GGTGGTTCTG 
TGCTGTAAAG TGTGTGCTCG ACCCAAACCC ACAATCACCT TGAAAGGACC TGATCAAAGT 
CTGCTGGCCA ACAATAACCG CTTCACTATT AATATCAGAG AAACCGGTGA TATAGTGTTG 
AAAATCTGCA ATCTAATGCC GCAGGACACT GGCATCTACA CGTGTGTTGC TGTGAATGAC 
CATGGTACAG CCTCCTCATC TGCATCCATT AAAGTACAAG GTATCCCAGC AGCCCCTGCT 
CGACCGGTGG CACAGGAGGC AAGCAGCACT GCAGTGATTG TCCACTGGCT TCCTCCAGCC 
AGCTCAGCAA ATTCTGCCAT CTCCAGCTAC ACTGTGGAAT ATAGGCAGGA AGATTCTCTG 
GTATGGCAGC AGTTGGTGGT CTCCACGGCA GATGTGTTTG TGAAGATTGA CAATCTGATT 
CCTGGTGGGC ACTACCAGTT CAGAGTCAGT GCCAGTAATC CCTGGGGCAC CAGCCCGCCT 
AGTGAACCCT CAAACATGGT GACATTATCC AGCACAGCAT CAACATATGA TGGCGCTGGA 
ATCCAGTGGA AAGACAACTT TGAATCTGCT TTTACAGAAT TATGTGAAAT TGGGCGGGGT 
CGTTTCTCTG TGGTGAGGAA GTGTCTCAGT AAAGCCACTA AGCAGGAGGT TGCTGTGAAG 
TTTGTGAATA AGAAGATGCA CAAGAAAGAA CAGGTGGCCC ATGAAGCTGA TATTCTACGC 
CATGTCCAGC ACCCCCAACT GGTGTCACTG ATTGATACAT ACGAGTCGCC TACAGCCTAC 
ATGCTGATTC TTGAACTTGT TGAGGATGGA CGTCTTCTTG ACTATCTGGT AGCTCATGAT 
GAGCTGATGG AGGAGAAAGT GGCATTCTTT ATTAAAGATA CACTGGAAGC TCTGCAGCAC 
TTGCACACCT GCAGAGTGGC TCATCTAGAC CTCAAGCCTG AAAACTTGTT GGTGGACCTC 
CACGTTCCGG TACCCTGCGT GAAGCTCTCA GATCTAGGTG ATGCTGTACA GGTTTCAAGT 
CACCGCTACG TACATCTCCT CCTGGGTAAC CCCGAGTTCG CTGCTCCAGA GCTCATCCAG 
GGCACTCCAG TGTCCCTCTC TACAGATGTG TGGAGTGTAG GGGTGCTGGC CTACGTCATG 
CTTAGTGGAG TCTCCCCATT CCTGGATGAG AGTCTGGAGG AGACCTGCGT CAACATCTGC 
AGGCTGGACT TCTGCTTTCC TCAAGAGTAC TTCTCCGATA TAAGCCAGGC AGCCAAAGAC 
TTCATTGTGT CCACGCTTAA CCAGGATCCC AGGAAGAGGC CAAGCTCGGC CTCGTGTCTT 
CAGCACCCAT GGGTCAGTGC TCATAGTGGA GATTACTCCA AAACACCTCT GGATACTGTC 
AGACTCGCTG CATTTATAGA CAGACGCAAA CAGCTACATG ATGTCAGGCC TGTCACAAAC 
ATTAAAGGAA TGGTCAGCAG CAGTATGGGA CACACCTTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302729.1
CDS	50..4111
Translation	MPKWMSFKLFKESSRLSGGCELTVVLQDFTASSSSELSIQTGQTVELLERPSDRPGWCLVRTTEKTPPQEGLVPSSALCISHSRSSVEMDCFFPAGKENYTVSGPEGKTESVATLQPQPSLNSLQSSSPGPKRSGNTLKKWLTSPVRRLSHGSNIKKIPSKQKQKRDGRKSIDMGPPELQDDTLEERVQKEEGTLSKSSSGMHSGGEEEPEDESHTPLPPPMEIIKDPSAQEEKSSVLLASRQSSADVPSAAELVSAIEKLVKTKMTLEPGSYPGFSSVNPAEQSPIPPRNPELEQKAKALRGRMFVVKELIQTEKDYVKDLGIVVEGFMKRIEEKGVPDNMKGKDKIVFGNIHQIYDWHKDFFVGELEKCLEDHEKLPELFCKHERRLHMYVVYCQNKPKSEFIVAEYDSYFEGIQQDINSRLSISDFLIKPIQRITKYQLLLKDVLKYSTKAGLDCQDIENAVDLMSQVPKLCNDMMNLGRLQGYEGKLTSQGKLLQQETFFVTEQDSGVLSRSKERRVFLFEQIVIFSELLRKGSSTPGYQFKKSIKVSFLDLEEHVENDPCKFVLSCRGSAERFTLQAANTDIKQVWVQHITQLLDLQSNFLSALQSPIEYQKERSGSQSLTRNSSGRMGQSNSRPCSTVSLGAEKPSLSGRSSTPLKLSTSNGGPCSERYRRQFEGLGCNGTSSSLMVTQDYTALKENEICVTQGEAVQVLATNQQNMYLVYRPANSQSPPAEGWVPGHILGPVTKPLIDTTADASIKKSLSWNTLRARKRAEKDSTPLKSDFKVENGLRKPKEILSSKVKVKESRSSDESECEDELDPKTSMELLNPNFIQEVAPEFLTPLTDVTCALGETVVLCCKVCARPKPTITLKGPDQSLLANNNRFTINIRETGDIVLKICNLMPQDTGIYTCVAVNDHGTASSSASIKVQGIPAAPARPVAQEASSTAVIVHWLPPASSANSAISSYTVEYRQEDSLVWQQLVVSTADVFVKIDNLIPGGHYQFRVSASNPWGTSPPSEPSNMVTLSSTASTYDGAGIQWKDNFESAFTELCEIGRGRFSVVRKCLSKATKQEVAVKFVNKKMHKKEQVAHEADILRHVQHPQLVSLIDTYESPTAYMLILELVEDGRLLDYLVAHDELMEEKVAFFIKDTLEALQHLHTCRVAHLDLKPENLLVDLHVPVPCVKLSDLGDAVQVSSHRYVHLLLGNPEFAAPELIQGTPVSLSTDVWSVGVLAYVMLSGVSPFLDESLEETCVNICRLDFCFPQEYFSDISQAAKDFIVSTLNQDPRKRPSSASCLQHPWVSAHSGDYSKTPLDTVRLAAFIDRRKQLHDVRPVTNIKGMVSSSMGHTL

Target ORF information:

RefSeq Version	XM_009304454.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kalirin RhoGEF kinase a (kalrna), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304454.3

ORF Insert Sequence:

ATGCCAAAGT GGATGAGTTT TAAACTTTTC AAAGAGAGCA GTCGTCTCTC TGGAGGGTGT 
GAGCTGACGG TGGTCCTGCA GGACTTTACA GCAAGCAGCT CATCAGAGCT CAGCATCCAG 
ACGGGTCAGA CAGTGGAGCT GCTGGAGCGG CCCAGTGACC GTCCAGGATG GTGTCTGGTA 
CGGACCACCG AAAAGACGCC TCCTCAAGAG GGACTGGTGC CCAGCAGTGC TCTGTGTATC 
TCGCACTCAC GCAGCAGTGT GGAGATGGAC TGCTTTTTCC CAGCAGGCAA AGAAAACTAT 
ACAGTTAGTG GCCCAGAAGG AAAGACAGAG TCAGTGGCCA CACTGCAGCC ACAGCCATCC 
CTGAACTCTC TGCAGAGTAG CTCTCCTGGA CCCAAGCGCT CTGGGAATAC ACTGAAGAAA 
TGGCTGACCA GTCCTGTCCG ACGTCTAAGT CATGGCAGTA ACATCAAGAA GATCCCAAGT 
AAACAGAAAC AGAAACGTGA TGGTCGCAAG AGTATTGATA TGGGGCCGCC CGAACTACAG 
GATGACACCT TGGAAGAGCG AGTGCAAAAA GAAGAGGGCA CACTCTCCAA GTCTTCGTCT 
GGGATGCACA GTGGAGGAGA GGAAGAGCCT GAGGACGAGT CTCACACTCC TCTACCTCCA 
CCAATGGAGA TCATTAAAGA CCCGTCGGCT CAGGAAGAGA AGTCGTCTGT GCTTCTGGCT 
TCCCGGCAGA GCTCGGCTGA TGTCCCCAGT GCTGCTGAGC TTGTCAGTGC TATAGAAAAA 
CTGGTCAAAA CTAAAATGAC ACTGGAGCCA GGCTCATACC CGGGATTCAG CTCAGTCAAT 
CCAGCAGAGC AGAGCCCGAT TCCACCCAGA AACCCAGAAC TAGAGCAGAA AGCCAAAGCG 
CTACGAGGAC GAATGTTTGT AGTCAAGGAA TTAATCCAAA CGGAAAAAGA CTATGTTAAA 
GATCTGGGAA TTGTGGTTGA GGGGTTTATG AAGAGAATCG AGGAGAAAGG AGTTCCTGAT 
AACATGAAAG GAAAAGATAA GATCGTCTTC GGAAACATTC ACCAAATCTA TGACTGGCAC 
AAAGATTTTT TTGTTGGAGA ACTGGAGAAA TGCCTGGAAG ATCACGAAAA ACTGCCTGAA 
CTGTTCTGTA AACATGAGAG ACGGTTACAC ATGTATGTAG TTTACTGTCA GAACAAACCC 
AAATCCGAGT TCATCGTTGC AGAATATGAT TCATACTTTG AGGGGATTCA GCAAGACATT 
AACTCCAGAC TGAGCATCAG CGATTTTCTC ATTAAACCCA TTCAGAGAAT TACTAAATAC 
CAGCTTTTAT TAAAGGATGT TTTGAAGTAC AGTACAAAAG CAGGACTGGA TTGTCAGGAT 
ATAGAGAATG CAGTTGACCT GATGTCCCAG GTTCCCAAGC TTTGCAATGA CATGATGAAT 
CTGGGAAGAC TGCAGGGATA TGAGGGGAAG TTGACGAGTC AAGGAAAACT CTTGCAGCAG 
GAGACGTTCT TTGTGACCGA GCAGGACTCT GGCGTTCTGT CCCGCAGTAA AGAGCGCAGG 
GTTTTCCTGT TCGAACAAAT TGTCATCTTC AGCGAGCTGT TACGTAAAGG CTCTTCCACT 
CCAGGGTACC AGTTCAAGAA GAGCATAAAG GTGAGCTTCC TTGACTTGGA AGAGCATGTT 
GAAAATGATC CATGCAAGTT TGTGCTGTCG TGTCGTGGGT CGGCAGAACG TTTTACCCTT 
CAAGCAGCAA ACACGGACAT TAAACAAGTC TGGGTTCAGC ATATCACCCA ACTACTAGAT 
TTACAAAGCA ACTTCTTGTC AGCTCTGCAG TCTCCTATTG AGTATCAGAA GGAGCGTAGT 
GGCTCTCAAT CTCTGACCCG GAACTCTTCT GGGCGGATGG GTCAGTCCAA CAGCAGGCCC 
TGCTCCACGG TTTCACTCGG AGCAGAGAAA CCCTCTCTGT CTGGACGGAG CTCCACCCCG 
CTAAAGCTAT CTACCTCCAA TGGTGGTCCA TGCTCTGAAA GATACCGCAG GCAGTTTGAA 
GGCTTGGGCT GCAACGGTAC ATCGTCCTCC TTGATGGTGA CTCAAGATTA TACTGCACTG 
AAAGAGAATG AGATCTGTGT CACACAGGGT GAGGCCGTGC AGGTACTGGC CACTAACCAG 
CAGAACATGT ACCTTGTGTA CCGACCCGCA AATAGCCAAT CACCACCTGC AGAGGGCTGG 
GTGCCGGGAC ACATCCTAGG CCCCGTCACA AAACCTCTCA TAGACACTAC TGCAGACGCA 
AGCATCAAGA AATCTCTTTC GTGGAACACA CTTCGTGCTC GTAAGCGTGC AGAAAAGGAC 
AGCACGCCCC TCAAAAGTGA CTTTAAAGTT GAGAATGGAC TGCGTAAGCC AAAGGAAATT 
CTCAGCAGCA AAGTCAAAGT GAAAGAGTCC CGCAGTTCTG ATGAGTCTGA ATGTGAGGAT 
GAACTAGACC CCAAAACTAG TATGGAGTTA CTGAACCCTA ATTTCATCCA AGAGGTCGCT 
CCAGAGTTTC TGACGCCTCT AACAGACGTG ACGTGTGCTC TGGGAGAGAC GGTGGTTCTG 
TGCTGTAAAG TGTGTGCTCG ACCCAAACCC ACAATCACCT TGAAAGGACC TGATCAAAGT 
CTGCTGGCCA ACAATAACCG CTTCACTATT AATATCAGAG AAACCGGTGA TATAGTGTTG 
AAAATCTGCA ATCTAATGCC GCAGGACACT GGCATCTACA CGTGTGTTGC TGTGAATGAC 
CATGGTACAG CCTCCTCATC TGCATCCATT AAAGTACAAG GTATCCCAGC AGCCCCTGCT 
CGACCGGTGG CACAGGAGGC AAGCAGCACT GCAGTGATTG TCCACTGGCT TCCTCCAGCC 
AGCTCAGCAA ATTCTGCCAT CTCCAGCTAC ACTGTGGAAT ATAGGCAGGA AGATTCTCTG 
GTATGGCAGC AGTTGGTGGT CTCCACGGCA GATGTGTTTG TGAAGATTGA CAATCTGATT 
CCTGGTGGGC ACTACCAGTT CAGAGTCAGT GCCAGTAATC CCTGGGGCAC CAGCCCGCCT 
AGTGAACCCT CAAACATGGT GACATTATCC AGCACAGCAT CAACATATGA TGGCGCTGGA 
ATCCAGTGGA AAGACAACTT TGAATCTGCT TTTACAGAAT TATGTGAAAT TGGGCGGGGT 
CGTTTCTCTG TGGTGAGGAA GTGTCTCAGT AAAGCCACTA AGCAGGAGGT TGCTGTGAAG 
TTTGTGAATA AGAAGATGCA CAAGAAAGAA CAGGTGGCCC ATGAAGCTGA TATTCTACGC 
CATGTCCAGC ACCCCCAACT GGTGTCACTG ATTGATACAT ACGAGTCGCC TACAGCCTAC 
ATGCTGATTC TTGAACTTGT TGAGGATGGA CGTCTTCTTG ACTATCTGGT AGCTCATGAT 
GAGCTGATGG AGGAGAAAGT GGCATTCTTT ATTAAAGATA CACTGGAAGC TCTGCAGCAC 
TTGCACACCT GCAGAGTGGC TCATCTAGAC CTCAAGCCTG AAAACTTGTT GGTGGACCTC 
CACGTTCCGG TACCCTGCGT GAAGCTCTCA GATCTAGGTG ATGCTGTACA GGTTTCAAGT 
CACCGCTACG TACATCTCCT CCTGGGTAAC CCCGAGTTCG CTGCTCCAGA GCTCATCCAG 
GGCACTCCAG TGTCCCTCTC TACAGATGTG TGGAGTGTAG GGGTGCTGGC CTACGTCATG 
CTTAGTGGAG TCTCCCCATT CCTGGATGAG AGTCTGGAGG AGACCTGCGT CAACATCTGC 
AGGCTGGACT TCTGCTTTCC TCAAGAGTAC TTCTCCGATA TAAGCCAGGC AGCCAAAGAC 
TTCATTGTGT CCACGCTTAA CCAGGATCCC AGGAAGAGGC CAAGCTCGGC CTCGTGTCTT 
CAGCACCCAT GGGTCAGTGC TCATAGTGGA GATTACTCCA AAACACCTCT GGATACTGTC 
AGACTCGCTG CATTTATAGA CAGACGCAAA CAGCTACATG ATGTCAGGCC TGTCACAAAC 
ATTAAAGGAA TGGTCAGCAG CAGTATGGGA CACACCTTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kalrna ( XM_009304454.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302729.1 Danio rerio kalirin, RhoGEF kinase a (kalrna), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa48698
Clone ID Related Accession (Same CDS sequence)	XM_009304454.3 , XM_009304454.2
Accession Version	XM_009304454.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4062bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kalirin isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304454.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCCAAAGT GGATGAGTTT TAAACTTTTC AAAGAGAGCA GTCGTCTCTC TGGAGGGTGT 
GAGCTGACGG TGGTCCTGCA GGACTTTACA GCAAGCAGCT CATCAGAGCT CAGCATCCAG 
ACGGGTCAGA CAGTGGAGCT GCTGGAGCGG CCCAGTGACC GTCCAGGATG GTGTCTGGTA 
CGGACCACCG AAAAGACGCC TCCTCAAGAG GGACTGGTGC CCAGCAGTGC TCTGTGTATC 
TCGCACTCAC GCAGCAGTGT GGAGATGGAC TGCTTTTTCC CAGCAGGCAA AGAAAACTAT 
ACAGTTAGTG GCCCAGAAGG AAAGACAGAG TCAGTGGCCA CACTGCAGCC ACAGCCATCC 
CTGAACTCTC TGCAGAGTAG CTCTCCTGGA CCCAAGCGCT CTGGGAATAC ACTGAAGAAA 
TGGCTGACCA GTCCTGTCCG ACGTCTAAGT CATGGCAGTA ACATCAAGAA GATCCCAAGT 
AAACAGAAAC AGAAACGTGA TGGTCGCAAG AGTATTGATA TGGGGCCGCC CGAACTACAG 
GATGACACCT TGGAAGAGCG AGTGCAAAAA GAAGAGGGCA CACTCTCCAA GTCTTCGTCT 
GGGATGCACA GTGGAGGAGA GGAAGAGCCT GAGGACGAGT CTCACACTCC TCTACCTCCA 
CCAATGGAGA TCATTAAAGA CCCGTCGGCT CAGGAAGAGA AGTCGTCTGT GCTTCTGGCT 
TCCCGGCAGA GCTCGGCTGA TGTCCCCAGT GCTGCTGAGC TTGTCAGTGC TATAGAAAAA 
CTGGTCAAAA CTAAAATGAC ACTGGAGCCA GGCTCATACC CGGGATTCAG CTCAGTCAAT 
CCAGCAGAGC AGAGCCCGAT TCCACCCAGA AACCCAGAAC TAGAGCAGAA AGCCAAAGCG 
CTACGAGGAC GAATGTTTGT AGTCAAGGAA TTAATCCAAA CGGAAAAAGA CTATGTTAAA 
GATCTGGGAA TTGTGGTTGA GGGGTTTATG AAGAGAATCG AGGAGAAAGG AGTTCCTGAT 
AACATGAAAG GAAAAGATAA GATCGTCTTC GGAAACATTC ACCAAATCTA TGACTGGCAC 
AAAGATTTTT TTGTTGGAGA ACTGGAGAAA TGCCTGGAAG ATCACGAAAA ACTGCCTGAA 
CTGTTCTGTA AACATGAGAG ACGGTTACAC ATGTATGTAG TTTACTGTCA GAACAAACCC 
AAATCCGAGT TCATCGTTGC AGAATATGAT TCATACTTTG AGGGGATTCA GCAAGACATT 
AACTCCAGAC TGAGCATCAG CGATTTTCTC ATTAAACCCA TTCAGAGAAT TACTAAATAC 
CAGCTTTTAT TAAAGGATGT TTTGAAGTAC AGTACAAAAG CAGGACTGGA TTGTCAGGAT 
ATAGAGAATG CAGTTGACCT GATGTCCCAG GTTCCCAAGC TTTGCAATGA CATGATGAAT 
CTGGGAAGAC TGCAGGGATA TGAGGGGAAG TTGACGAGTC AAGGAAAACT CTTGCAGCAG 
GAGACGTTCT TTGTGACCGA GCAGGACTCT GGCGTTCTGT CCCGCAGTAA AGAGCGCAGG 
GTTTTCCTGT TCGAACAAAT TGTCATCTTC AGCGAGCTGT TACGTAAAGG CTCTTCCACT 
CCAGGGTACC AGTTCAAGAA GAGCATAAAG GTGAGCTTCC TTGACTTGGA AGAGCATGTT 
GAAAATGATC CATGCAAGTT TGTGCTGTCG TGTCGTGGGT CGGCAGAACG TTTTACCCTT 
CAAGCAGCAA ACACGGACAT TAAACAAGTC TGGGTTCAGC ATATCACCCA ACTACTAGAT 
TTACAAAGCA ACTTCTTGTC AGCTCTGCAG TCTCCTATTG AGTATCAGAA GGAGCGTAGT 
GGCTCTCAAT CTCTGACCCG GAACTCTTCT GGGCGGATGG GTCAGTCCAA CAGCAGGCCC 
TGCTCCACGG TTTCACTCGG AGCAGAGAAA CCCTCTCTGT CTGGACGGAG CTCCACCCCG 
CTAAAGCTAT CTACCTCCAA TGGTGGTCCA TGCTCTGAAA GATACCGCAG GCAGTTTGAA 
GGCTTGGGCT GCAACGGTAC ATCGTCCTCC TTGATGGTGA CTCAAGATTA TACTGCACTG 
AAAGAGAATG AGATCTGTGT CACACAGGGT GAGGCCGTGC AGGTACTGGC CACTAACCAG 
CAGAACATGT ACCTTGTGTA CCGACCCGCA AATAGCCAAT CACCACCTGC AGAGGGCTGG 
GTGCCGGGAC ACATCCTAGG CCCCGTCACA AAACCTCTCA TAGACACTAC TGCAGACGCA 
AGCATCAAGA AATCTCTTTC GTGGAACACA CTTCGTGCTC GTAAGCGTGC AGAAAAGGAC 
AGCACGCCCC TCAAAAGTGA CTTTAAAGTT GAGAATGGAC TGCGTAAGCC AAAGGAAATT 
CTCAGCAGCA AAGTCAAAGT GAAAGAGTCC CGCAGTTCTG ATGAGTCTGA ATGTGAGGAT 
GAACTAGACC CCAAAACTAG TATGGAGTTA CTGAACCCTA ATTTCATCCA AGAGGTCGCT 
CCAGAGTTTC TGACGCCTCT AACAGACGTG ACGTGTGCTC TGGGAGAGAC GGTGGTTCTG 
TGCTGTAAAG TGTGTGCTCG ACCCAAACCC ACAATCACCT TGAAAGGACC TGATCAAAGT 
CTGCTGGCCA ACAATAACCG CTTCACTATT AATATCAGAG AAACCGGTGA TATAGTGTTG 
AAAATCTGCA ATCTAATGCC GCAGGACACT GGCATCTACA CGTGTGTTGC TGTGAATGAC 
CATGGTACAG CCTCCTCATC TGCATCCATT AAAGTACAAG GTATCCCAGC AGCCCCTGCT 
CGACCGGTGG CACAGGAGGC AAGCAGCACT GCAGTGATTG TCCACTGGCT TCCTCCAGCC 
AGCTCAGCAA ATTCTGCCAT CTCCAGCTAC ACTGTGGAAT ATAGGCAGGA AGATTCTCTG 
GTATGGCAGC AGTTGGTGGT CTCCACGGCA GATGTGTTTG TGAAGATTGA CAATCTGATT 
CCTGGTGGGC ACTACCAGTT CAGAGTCAGT GCCAGTAATC CCTGGGGCAC CAGCCCGCCT 
AGTGAACCCT CAAACATGGT GACATTATCC AGCACAGCAT CAACATATGA TGGCGCTGGA 
ATCCAGTGGA AAGACAACTT TGAATCTGCT TTTACAGAAT TATGTGAAAT TGGGCGGGGT 
CGTTTCTCTG TGGTGAGGAA GTGTCTCAGT AAAGCCACTA AGCAGGAGGT TGCTGTGAAG 
TTTGTGAATA AGAAGATGCA CAAGAAAGAA CAGGTGGCCC ATGAAGCTGA TATTCTACGC 
CATGTCCAGC ACCCCCAACT GGTGTCACTG ATTGATACAT ACGAGTCGCC TACAGCCTAC 
ATGCTGATTC TTGAACTTGT TGAGGATGGA CGTCTTCTTG ACTATCTGGT AGCTCATGAT 
GAGCTGATGG AGGAGAAAGT GGCATTCTTT ATTAAAGATA CACTGGAAGC TCTGCAGCAC 
TTGCACACCT GCAGAGTGGC TCATCTAGAC CTCAAGCCTG AAAACTTGTT GGTGGACCTC 
CACGTTCCGG TACCCTGCGT GAAGCTCTCA GATCTAGGTG ATGCTGTACA GGTTTCAAGT 
CACCGCTACG TACATCTCCT CCTGGGTAAC CCCGAGTTCG CTGCTCCAGA GCTCATCCAG 
GGCACTCCAG TGTCCCTCTC TACAGATGTG TGGAGTGTAG GGGTGCTGGC CTACGTCATG 
CTTAGTGGAG TCTCCCCATT CCTGGATGAG AGTCTGGAGG AGACCTGCGT CAACATCTGC 
AGGCTGGACT TCTGCTTTCC TCAAGAGTAC TTCTCCGATA TAAGCCAGGC AGCCAAAGAC 
TTCATTGTGT CCACGCTTAA CCAGGATCCC AGGAAGAGGC CAAGCTCGGC CTCGTGTCTT 
CAGCACCCAT GGGTCAGTGC TCATAGTGGA GATTACTCCA AAACACCTCT GGATACTGTC 
AGACTCGCTG CATTTATAGA CAGACGCAAA CAGCTACATG ATGTCAGGCC TGTCACAAAC 
ATTAAAGGAA TGGTCAGCAG CAGTATGGGA CACACCTTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302729.1
CDS	29..4090
Translation	MPKWMSFKLFKESSRLSGGCELTVVLQDFTASSSSELSIQTGQTVELLERPSDRPGWCLVRTTEKTPPQEGLVPSSALCISHSRSSVEMDCFFPAGKENYTVSGPEGKTESVATLQPQPSLNSLQSSSPGPKRSGNTLKKWLTSPVRRLSHGSNIKKIPSKQKQKRDGRKSIDMGPPELQDDTLEERVQKEEGTLSKSSSGMHSGGEEEPEDESHTPLPPPMEIIKDPSAQEEKSSVLLASRQSSADVPSAAELVSAIEKLVKTKMTLEPGSYPGFSSVNPAEQSPIPPRNPELEQKAKALRGRMFVVKELIQTEKDYVKDLGIVVEGFMKRIEEKGVPDNMKGKDKIVFGNIHQIYDWHKDFFVGELEKCLEDHEKLPELFCKHERRLHMYVVYCQNKPKSEFIVAEYDSYFEGIQQDINSRLSISDFLIKPIQRITKYQLLLKDVLKYSTKAGLDCQDIENAVDLMSQVPKLCNDMMNLGRLQGYEGKLTSQGKLLQQETFFVTEQDSGVLSRSKERRVFLFEQIVIFSELLRKGSSTPGYQFKKSIKVSFLDLEEHVENDPCKFVLSCRGSAERFTLQAANTDIKQVWVQHITQLLDLQSNFLSALQSPIEYQKERSGSQSLTRNSSGRMGQSNSRPCSTVSLGAEKPSLSGRSSTPLKLSTSNGGPCSERYRRQFEGLGCNGTSSSLMVTQDYTALKENEICVTQGEAVQVLATNQQNMYLVYRPANSQSPPAEGWVPGHILGPVTKPLIDTTADASIKKSLSWNTLRARKRAEKDSTPLKSDFKVENGLRKPKEILSSKVKVKESRSSDESECEDELDPKTSMELLNPNFIQEVAPEFLTPLTDVTCALGETVVLCCKVCARPKPTITLKGPDQSLLANNNRFTINIRETGDIVLKICNLMPQDTGIYTCVAVNDHGTASSSASIKVQGIPAAPARPVAQEASSTAVIVHWLPPASSANSAISSYTVEYRQEDSLVWQQLVVSTADVFVKIDNLIPGGHYQFRVSASNPWGTSPPSEPSNMVTLSSTASTYDGAGIQWKDNFESAFTELCEIGRGRFSVVRKCLSKATKQEVAVKFVNKKMHKKEQVAHEADILRHVQHPQLVSLIDTYESPTAYMLILELVEDGRLLDYLVAHDELMEEKVAFFIKDTLEALQHLHTCRVAHLDLKPENLLVDLHVPVPCVKLSDLGDAVQVSSHRYVHLLLGNPEFAAPELIQGTPVSLSTDVWSVGVLAYVMLSGVSPFLDESLEETCVNICRLDFCFPQEYFSDISQAAKDFIVSTLNQDPRKRPSSASCLQHPWVSAHSGDYSKTPLDTVRLAAFIDRRKQLHDVRPVTNIKGMVSSSMGHTL

Target ORF information:

RefSeq Version	XM_009304454.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kalirin, RhoGEF kinase a (kalrna), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304454.2

ORF Insert Sequence:

ATGCCAAAGT GGATGAGTTT TAAACTTTTC AAAGAGAGCA GTCGTCTCTC TGGAGGGTGT 
GAGCTGACGG TGGTCCTGCA GGACTTTACA GCAAGCAGCT CATCAGAGCT CAGCATCCAG 
ACGGGTCAGA CAGTGGAGCT GCTGGAGCGG CCCAGTGACC GTCCAGGATG GTGTCTGGTA 
CGGACCACCG AAAAGACGCC TCCTCAAGAG GGACTGGTGC CCAGCAGTGC TCTGTGTATC 
TCGCACTCAC GCAGCAGTGT GGAGATGGAC TGCTTTTTCC CAGCAGGCAA AGAAAACTAT 
ACAGTTAGTG GCCCAGAAGG AAAGACAGAG TCAGTGGCCA CACTGCAGCC ACAGCCATCC 
CTGAACTCTC TGCAGAGTAG CTCTCCTGGA CCCAAGCGCT CTGGGAATAC ACTGAAGAAA 
TGGCTGACCA GTCCTGTCCG ACGTCTAAGT CATGGCAGTA ACATCAAGAA GATCCCAAGT 
AAACAGAAAC AGAAACGTGA TGGTCGCAAG AGTATTGATA TGGGGCCGCC CGAACTACAG 
GATGACACCT TGGAAGAGCG AGTGCAAAAA GAAGAGGGCA CACTCTCCAA GTCTTCGTCT 
GGGATGCACA GTGGAGGAGA GGAAGAGCCT GAGGACGAGT CTCACACTCC TCTACCTCCA 
CCAATGGAGA TCATTAAAGA CCCGTCGGCT CAGGAAGAGA AGTCGTCTGT GCTTCTGGCT 
TCCCGGCAGA GCTCGGCTGA TGTCCCCAGT GCTGCTGAGC TTGTCAGTGC TATAGAAAAA 
CTGGTCAAAA CTAAAATGAC ACTGGAGCCA GGCTCATACC CGGGATTCAG CTCAGTCAAT 
CCAGCAGAGC AGAGCCCGAT TCCACCCAGA AACCCAGAAC TAGAGCAGAA AGCCAAAGCG 
CTACGAGGAC GAATGTTTGT AGTCAAGGAA TTAATCCAAA CGGAAAAAGA CTATGTTAAA 
GATCTGGGAA TTGTGGTTGA GGGGTTTATG AAGAGAATCG AGGAGAAAGG AGTTCCTGAT 
AACATGAAAG GAAAAGATAA GATCGTCTTC GGAAACATTC ACCAAATCTA TGACTGGCAC 
AAAGATTTTT TTGTTGGAGA ACTGGAGAAA TGCCTGGAAG ATCACGAAAA ACTGCCTGAA 
CTGTTCTGTA AACATGAGAG ACGGTTACAC ATGTATGTAG TTTACTGTCA GAACAAACCC 
AAATCCGAGT TCATCGTTGC AGAATATGAT TCATACTTTG AGGGGATTCA GCAAGACATT 
AACTCCAGAC TGAGCATCAG CGATTTTCTC ATTAAACCCA TTCAGAGAAT TACTAAATAC 
CAGCTTTTAT TAAAGGATGT TTTGAAGTAC AGTACAAAAG CAGGACTGGA TTGTCAGGAT 
ATAGAGAATG CAGTTGACCT GATGTCCCAG GTTCCCAAGC TTTGCAATGA CATGATGAAT 
CTGGGAAGAC TGCAGGGATA TGAGGGGAAG TTGACGAGTC AAGGAAAACT CTTGCAGCAG 
GAGACGTTCT TTGTGACCGA GCAGGACTCT GGCGTTCTGT CCCGCAGTAA AGAGCGCAGG 
GTTTTCCTGT TCGAACAAAT TGTCATCTTC AGCGAGCTGT TACGTAAAGG CTCTTCCACT 
CCAGGGTACC AGTTCAAGAA GAGCATAAAG GTGAGCTTCC TTGACTTGGA AGAGCATGTT 
GAAAATGATC CATGCAAGTT TGTGCTGTCG TGTCGTGGGT CGGCAGAACG TTTTACCCTT 
CAAGCAGCAA ACACGGACAT TAAACAAGTC TGGGTTCAGC ATATCACCCA ACTACTAGAT 
TTACAAAGCA ACTTCTTGTC AGCTCTGCAG TCTCCTATTG AGTATCAGAA GGAGCGTAGT 
GGCTCTCAAT CTCTGACCCG GAACTCTTCT GGGCGGATGG GTCAGTCCAA CAGCAGGCCC 
TGCTCCACGG TTTCACTCGG AGCAGAGAAA CCCTCTCTGT CTGGACGGAG CTCCACCCCG 
CTAAAGCTAT CTACCTCCAA TGGTGGTCCA TGCTCTGAAA GATACCGCAG GCAGTTTGAA 
GGCTTGGGCT GCAACGGTAC ATCGTCCTCC TTGATGGTGA CTCAAGATTA TACTGCACTG 
AAAGAGAATG AGATCTGTGT CACACAGGGT GAGGCCGTGC AGGTACTGGC CACTAACCAG 
CAGAACATGT ACCTTGTGTA CCGACCCGCA AATAGCCAAT CACCACCTGC AGAGGGCTGG 
GTGCCGGGAC ACATCCTAGG CCCCGTCACA AAACCTCTCA TAGACACTAC TGCAGACGCA 
AGCATCAAGA AATCTCTTTC GTGGAACACA CTTCGTGCTC GTAAGCGTGC AGAAAAGGAC 
AGCACGCCCC TCAAAAGTGA CTTTAAAGTT GAGAATGGAC TGCGTAAGCC AAAGGAAATT 
CTCAGCAGCA AAGTCAAAGT GAAAGAGTCC CGCAGTTCTG ATGAGTCTGA ATGTGAGGAT 
GAACTAGACC CCAAAACTAG TATGGAGTTA CTGAACCCTA ATTTCATCCA AGAGGTCGCT 
CCAGAGTTTC TGACGCCTCT AACAGACGTG ACGTGTGCTC TGGGAGAGAC GGTGGTTCTG 
TGCTGTAAAG TGTGTGCTCG ACCCAAACCC ACAATCACCT TGAAAGGACC TGATCAAAGT 
CTGCTGGCCA ACAATAACCG CTTCACTATT AATATCAGAG AAACCGGTGA TATAGTGTTG 
AAAATCTGCA ATCTAATGCC GCAGGACACT GGCATCTACA CGTGTGTTGC TGTGAATGAC 
CATGGTACAG CCTCCTCATC TGCATCCATT AAAGTACAAG GTATCCCAGC AGCCCCTGCT 
CGACCGGTGG CACAGGAGGC AAGCAGCACT GCAGTGATTG TCCACTGGCT TCCTCCAGCC 
AGCTCAGCAA ATTCTGCCAT CTCCAGCTAC ACTGTGGAAT ATAGGCAGGA AGATTCTCTG 
GTATGGCAGC AGTTGGTGGT CTCCACGGCA GATGTGTTTG TGAAGATTGA CAATCTGATT 
CCTGGTGGGC ACTACCAGTT CAGAGTCAGT GCCAGTAATC CCTGGGGCAC CAGCCCGCCT 
AGTGAACCCT CAAACATGGT GACATTATCC AGCACAGCAT CAACATATGA TGGCGCTGGA 
ATCCAGTGGA AAGACAACTT TGAATCTGCT TTTACAGAAT TATGTGAAAT TGGGCGGGGT 
CGTTTCTCTG TGGTGAGGAA GTGTCTCAGT AAAGCCACTA AGCAGGAGGT TGCTGTGAAG 
TTTGTGAATA AGAAGATGCA CAAGAAAGAA CAGGTGGCCC ATGAAGCTGA TATTCTACGC 
CATGTCCAGC ACCCCCAACT GGTGTCACTG ATTGATACAT ACGAGTCGCC TACAGCCTAC 
ATGCTGATTC TTGAACTTGT TGAGGATGGA CGTCTTCTTG ACTATCTGGT AGCTCATGAT 
GAGCTGATGG AGGAGAAAGT GGCATTCTTT ATTAAAGATA CACTGGAAGC TCTGCAGCAC 
TTGCACACCT GCAGAGTGGC TCATCTAGAC CTCAAGCCTG AAAACTTGTT GGTGGACCTC 
CACGTTCCGG TACCCTGCGT GAAGCTCTCA GATCTAGGTG ATGCTGTACA GGTTTCAAGT 
CACCGCTACG TACATCTCCT CCTGGGTAAC CCCGAGTTCG CTGCTCCAGA GCTCATCCAG 
GGCACTCCAG TGTCCCTCTC TACAGATGTG TGGAGTGTAG GGGTGCTGGC CTACGTCATG 
CTTAGTGGAG TCTCCCCATT CCTGGATGAG AGTCTGGAGG AGACCTGCGT CAACATCTGC 
AGGCTGGACT TCTGCTTTCC TCAAGAGTAC TTCTCCGATA TAAGCCAGGC AGCCAAAGAC 
TTCATTGTGT CCACGCTTAA CCAGGATCCC AGGAAGAGGC CAAGCTCGGC CTCGTGTCTT 
CAGCACCCAT GGGTCAGTGC TCATAGTGGA GATTACTCCA AAACACCTCT GGATACTGTC 
AGACTCGCTG CATTTATAGA CAGACGCAAA CAGCTACATG ATGTCAGGCC TGTCACAAAC 
ATTAAAGGAA TGGTCAGCAG CAGTATGGGA CACACCTTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kalrna ( XM_009304455.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302730.1 Danio rerio kalirin, RhoGEF kinase a (kalrna), transcript variant X5, mRNA.

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CloneID	ODa48699
Clone ID Related Accession (Same CDS sequence)	XM_009304455.2 , XM_009304455.3
Accession Version	XM_009304455.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kalirin isoform X5
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304455.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCATGA GGGGTGGTAA GAGTGTGTGC TCCTGCTGTG ACTGGATCTT CATTAAATGC 
TGCTGCTGCT GTCATGTTGG AGAAAACTAT ACAGTTAGTG GCCCAGAAGG AAAGACAGAG 
TCAGTGGCCA CACTGCAGCC ACAGCCATCC CTGAACTCTC TGCAGAGTAG CTCTCCTGGA 
CCCAAGCGCT CTGGGAATAC ACTGAAGAAA TGGCTGACCA GTCCTGTCCG ACGTCTAAGT 
CATGGCAGTA ACATCAAGAA GATCCCAAGT AAACAGAAAC AGAAACGTGA TGGTCGCAAG 
AGTATTGATA TGGGGCCGCC CGAACTACAG GATGACACCT TGGAAGAGCG AGTGCAAAAA 
GAAGAGGGCA CACTCTCCAA GTCTTCGTCT GGGATGCACA GTGGAGGAGA GGAAGAGCCT 
GAGGACGAGT CTCACACTCC TCTACCTCCA CCAATGGAGA TCATTAAAGA CCCGTCGGCT 
CAGGAAGAGA AGTCGTCTGT GCTTCTGGCT TCCCGGCAGA GCTCGGCTGA TGTCCCCAGT 
GCTGCTGAGC TTGTCAGTGC TATAGAAAAA CTGGTCAAAA CTAAAATGAC ACTGGAGCCA 
GGCTCATACC CGGGATTCAG CTCAGTCAAT CCAGCAGAGC AGAGCCCGAT TCCACCCAGA 
AACCCAGAAC TAGAGCAGAA AGCCAAAGCG CTACGAGGAC GAATGTTTGT AGTCAAGGAA 
TTAATCCAAA CGGAAAAAGA CTATGTTAAA GATCTGGGAA TTGTGGTTGA GGGGTTTATG 
AAGAGAATCG AGGAGAAAGG AGTTCCTGAT AACATGAAAG GAAAAGATAA GATCGTCTTC 
GGAAACATTC ACCAAATCTA TGACTGGCAC AAAGATTTTT TTGTTGGAGA ACTGGAGAAA 
TGCCTGGAAG ATCACGAAAA ACTGCCTGAA CTGTTCTGTA AACATGAGAG ACGGTTACAC 
ATGTATGTAG TTTACTGTCA GAACAAACCC AAATCCGAGT TCATCGTTGC AGAATATGAT 
TCATACTTTG AGGGGATTCA GCAAGACATT AACTCCAGAC TGAGCATCAG CGATTTTCTC 
ATTAAACCCA TTCAGAGAAT TACTAAATAC CAGCTTTTAT TAAAGGATGT TTTGAAGTAC 
AGTACAAAAG CAGGACTGGA TTGTCAGGAT ATAGAGAATG CAGTTGACCT GATGTCCCAG 
GTTCCCAAGC TTTGCAATGA CATGATGAAT CTGGGAAGAC TGCAGGGATA TGAGGGGAAG 
TTGACGAGTC AAGGAAAACT CTTGCAGCAG GAGACGTTCT TTGTGACCGA GCAGGACTCT 
GGCGTTCTGT CCCGCAGTAA AGAGCGCAGG GTTTTCCTGT TCGAACAAAT TGTCATCTTC 
AGCGAGCTGT TACGTAAAGG CTCTTCCACT CCAGGGTACC AGTTCAAGAA GAGCATAAAG 
GTGAGCTTCC TTGACTTGGA AGAGCATGTT GAAAATGATC CATGCAAGTT TGTGCTGTCG 
TGTCGTGGGT CGGCAGAACG TTTTACCCTT CAAGCAGCAA ACACGGACAT TAAACAAGTC 
TGGGTTCAGC ATATCACCCA ACTACTAGAT TTACAAAGCA ACTTCTTGTC AGCTCTGCAG 
TCTCCTATTG AGTATCAGAA GGAGCGTAGT GGCTCTCAAT CTCTGACCCG GAACTCTTCT 
GGGCGGATGG GTCAGTCCAA CAGCAGGCCC TGCTCCACGG TTTCACTCGG AGCAGAGAAA 
CCCTCTCTGT CTGGACGGAG CTCCACCCCG CTAAAGCTAT CTACCTCCAA TGGTGGTCCA 
TGCTCTGAAA GATACCGCAG GCAGTTTGAA GGCTTGGGCT GCAACGGTAC ATCGTCCTCC 
TTGATGGTGA CTCAAGATTA TACTGCACTG AAAGAGAATG AGATCTGTGT CACACAGGGT 
GAGGCCGTGC AGGTACTGGC CACTAACCAG CAGAACATGT ACCTTGTGTA CCGACCCGCA 
AATAGCCAAT CACCACCTGC AGAGGGCTGG GTGCCGGGAC ACATCCTAGG CCCCGTCACA 
AAACCTCTCA TAGACACTAC TGCAGACGCA AGCATCAAGA AATCTCTTTC GTGGAACACA 
CTTCGTGCTC GTAAGCGTGC AGAAAAGGAC AGCACGCCCC TCAAAAGTGA CTTTAAAGTT 
GAGAATGGAC TGCGTAAGCC AAAGGAAATT CTCAGCAGCA AAGTCAAAGT GAAAGAGTCC 
CGCAGTTCTG ATGAGTCTGA ATGTGAGGAT GAACTAGACC CCAAAACTAG TATGGAGTTA 
CTGAACCCTA ATTTCATCCA AGAGGTCGCT CCAGAGTTTC TGACGCCTCT AACAGACGTG 
ACGTGTGCTC TGGGAGAGAC GGTGGTTCTG TGCTGTAAAG TGTGTGCTCG ACCCAAACCC 
ACAATCACCT TGAAAGGACC TGATCAAAGT CTGCTGGCCA ACAATAACCG CTTCACTATT 
AATATCAGAG AAACCGGTGA TATAGTGTTG AAAATCTGCA ATCTAATGCC GCAGGACACT 
GGCATCTACA CGTGTGTTGC TGTGAATGAC CATGGTACAG CCTCCTCATC TGCATCCATT 
AAAGTACAAG GTATCCCAGC AGCCCCTGCT CGACCGGTGG CACAGGAGGC AAGCAGCACT 
GCAGTGATTG TCCACTGGCT TCCTCCAGCC AGCTCAGCAA ATTCTGCCAT CTCCAGCTAC 
ACTGTGGAAT ATAGGCAGGA AGATTCTCTG GTATGGCAGC AGTTGGTGGT CTCCACGGCA 
GATGTGTTTG TGAAGATTGA CAATCTGATT CCTGGTGGGC ACTACCAGTT CAGAGTCAGT 
GCCAGTAATC CCTGGGGCAC CAGCCCGCCT AGTGAACCCT CAAACATGGT GACATTATCC 
AGCACAGCAT CAACATATGA TGGCGCTGGA ATCCAGTGGA AAGACAACTT TGAATCTGCT 
TTTACAGAAT TATGTGAAAT TGGGCGGGGT CGTTTCTCTG TGGTGAGGAA GTGTCTCAGT 
AAAGCCACTA AGCAGGAGGT TGCTGTGAAG TTTGTGAATA AGAAGATGCA CAAGAAAGAA 
CAGGTGGCCC ATGAAGCTGA TATTCTACGC CATGTCCAGC ACCCCCAACT GGTGTCACTG 
ATTGATACAT ACGAGTCGCC TACAGCCTAC ATGCTGATTC TTGAACTTGT TGAGGATGGA 
CGTCTTCTTG ACTATCTGGT AGCTCATGAT GAGCTGATGG AGGAGAAAGT GGCATTCTTT 
ATTAAAGATA CACTGGAAGC TCTGCAGCAC TTGCACACCT GCAGAGTGGC TCATCTAGAC 
CTCAAGCCTG AAAACTTGTT GGTGGACCTC CACGTTCCGG TACCCTGCGT GAAGCTCTCA 
GATCTAGGTG ATGCTGTACA GGTTTCAAGT CACCGCTACG TACATCTCCT CCTGGGTAAC 
CCCGAGTTCG CTGCTCCAGA GCTCATCCAG GGCACTCCAG TGTCCCTCTC TACAGATGTG 
TGGAGTGTAG GGGTGCTGGC CTACGTCATG CTTAGTGGAG TCTCCCCATT CCTGGATGAG 
AGTCTGGAGG AGACCTGCGT CAACATCTGC AGGCTGGACT TCTGCTTTCC TCAAGAGTAC 
TTCTCCGATA TAAGCCAGGC AGCCAAAGAC TTCATTGTGT CCACGCTTAA CCAGGATCCC 
AGGAAGAGGC CAAGCTCGGC CTCGTGTCTT CAGCACCCAT GGGTCAGTGC TCATAGTGGA 
GATTACTCCA AAACACCTCT GGATACTGTC AGACTCGCTG CATTTATAGA CAGACGCAAA 
CAGCTACATG ATGTCAGGCC TGTCACAAAC ATTAAAGGAA TGGTCAGCAG CAGTATGGGA 
CACACCTTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302730.1
CDS	55..3906
Translation	MVMRGGKSVCSCCDWIFIKCCCCCHVGENYTVSGPEGKTESVATLQPQPSLNSLQSSSPGPKRSGNTLKKWLTSPVRRLSHGSNIKKIPSKQKQKRDGRKSIDMGPPELQDDTLEERVQKEEGTLSKSSSGMHSGGEEEPEDESHTPLPPPMEIIKDPSAQEEKSSVLLASRQSSADVPSAAELVSAIEKLVKTKMTLEPGSYPGFSSVNPAEQSPIPPRNPELEQKAKALRGRMFVVKELIQTEKDYVKDLGIVVEGFMKRIEEKGVPDNMKGKDKIVFGNIHQIYDWHKDFFVGELEKCLEDHEKLPELFCKHERRLHMYVVYCQNKPKSEFIVAEYDSYFEGIQQDINSRLSISDFLIKPIQRITKYQLLLKDVLKYSTKAGLDCQDIENAVDLMSQVPKLCNDMMNLGRLQGYEGKLTSQGKLLQQETFFVTEQDSGVLSRSKERRVFLFEQIVIFSELLRKGSSTPGYQFKKSIKVSFLDLEEHVENDPCKFVLSCRGSAERFTLQAANTDIKQVWVQHITQLLDLQSNFLSALQSPIEYQKERSGSQSLTRNSSGRMGQSNSRPCSTVSLGAEKPSLSGRSSTPLKLSTSNGGPCSERYRRQFEGLGCNGTSSSLMVTQDYTALKENEICVTQGEAVQVLATNQQNMYLVYRPANSQSPPAEGWVPGHILGPVTKPLIDTTADASIKKSLSWNTLRARKRAEKDSTPLKSDFKVENGLRKPKEILSSKVKVKESRSSDESECEDELDPKTSMELLNPNFIQEVAPEFLTPLTDVTCALGETVVLCCKVCARPKPTITLKGPDQSLLANNNRFTINIRETGDIVLKICNLMPQDTGIYTCVAVNDHGTASSSASIKVQGIPAAPARPVAQEASSTAVIVHWLPPASSANSAISSYTVEYRQEDSLVWQQLVVSTADVFVKIDNLIPGGHYQFRVSASNPWGTSPPSEPSNMVTLSSTASTYDGAGIQWKDNFESAFTELCEIGRGRFSVVRKCLSKATKQEVAVKFVNKKMHKKEQVAHEADILRHVQHPQLVSLIDTYESPTAYMLILELVEDGRLLDYLVAHDELMEEKVAFFIKDTLEALQHLHTCRVAHLDLKPENLLVDLHVPVPCVKLSDLGDAVQVSSHRYVHLLLGNPEFAAPELIQGTPVSLSTDVWSVGVLAYVMLSGVSPFLDESLEETCVNICRLDFCFPQEYFSDISQAAKDFIVSTLNQDPRKRPSSASCLQHPWVSAHSGDYSKTPLDTVRLAAFIDRRKQLHDVRPVTNIKGMVSSSMGHTL

Target ORF information:

RefSeq Version	XM_009304455.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kalirin, RhoGEF kinase a (kalrna), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304455.2

ORF Insert Sequence:

ATGGTCATGA GGGGTGGTAA GAGTGTGTGC TCCTGCTGTG ACTGGATCTT CATTAAATGC 
TGCTGCTGCT GTCATGTTGG AGAAAACTAT ACAGTTAGTG GCCCAGAAGG AAAGACAGAG 
TCAGTGGCCA CACTGCAGCC ACAGCCATCC CTGAACTCTC TGCAGAGTAG CTCTCCTGGA 
CCCAAGCGCT CTGGGAATAC ACTGAAGAAA TGGCTGACCA GTCCTGTCCG ACGTCTAAGT 
CATGGCAGTA ACATCAAGAA GATCCCAAGT AAACAGAAAC AGAAACGTGA TGGTCGCAAG 
AGTATTGATA TGGGGCCGCC CGAACTACAG GATGACACCT TGGAAGAGCG AGTGCAAAAA 
GAAGAGGGCA CACTCTCCAA GTCTTCGTCT GGGATGCACA GTGGAGGAGA GGAAGAGCCT 
GAGGACGAGT CTCACACTCC TCTACCTCCA CCAATGGAGA TCATTAAAGA CCCGTCGGCT 
CAGGAAGAGA AGTCGTCTGT GCTTCTGGCT TCCCGGCAGA GCTCGGCTGA TGTCCCCAGT 
GCTGCTGAGC TTGTCAGTGC TATAGAAAAA CTGGTCAAAA CTAAAATGAC ACTGGAGCCA 
GGCTCATACC CGGGATTCAG CTCAGTCAAT CCAGCAGAGC AGAGCCCGAT TCCACCCAGA 
AACCCAGAAC TAGAGCAGAA AGCCAAAGCG CTACGAGGAC GAATGTTTGT AGTCAAGGAA 
TTAATCCAAA CGGAAAAAGA CTATGTTAAA GATCTGGGAA TTGTGGTTGA GGGGTTTATG 
AAGAGAATCG AGGAGAAAGG AGTTCCTGAT AACATGAAAG GAAAAGATAA GATCGTCTTC 
GGAAACATTC ACCAAATCTA TGACTGGCAC AAAGATTTTT TTGTTGGAGA ACTGGAGAAA 
TGCCTGGAAG ATCACGAAAA ACTGCCTGAA CTGTTCTGTA AACATGAGAG ACGGTTACAC 
ATGTATGTAG TTTACTGTCA GAACAAACCC AAATCCGAGT TCATCGTTGC AGAATATGAT 
TCATACTTTG AGGGGATTCA GCAAGACATT AACTCCAGAC TGAGCATCAG CGATTTTCTC 
ATTAAACCCA TTCAGAGAAT TACTAAATAC CAGCTTTTAT TAAAGGATGT TTTGAAGTAC 
AGTACAAAAG CAGGACTGGA TTGTCAGGAT ATAGAGAATG CAGTTGACCT GATGTCCCAG 
GTTCCCAAGC TTTGCAATGA CATGATGAAT CTGGGAAGAC TGCAGGGATA TGAGGGGAAG 
TTGACGAGTC AAGGAAAACT CTTGCAGCAG GAGACGTTCT TTGTGACCGA GCAGGACTCT 
GGCGTTCTGT CCCGCAGTAA AGAGCGCAGG GTTTTCCTGT TCGAACAAAT TGTCATCTTC 
AGCGAGCTGT TACGTAAAGG CTCTTCCACT CCAGGGTACC AGTTCAAGAA GAGCATAAAG 
GTGAGCTTCC TTGACTTGGA AGAGCATGTT GAAAATGATC CATGCAAGTT TGTGCTGTCG 
TGTCGTGGGT CGGCAGAACG TTTTACCCTT CAAGCAGCAA ACACGGACAT TAAACAAGTC 
TGGGTTCAGC ATATCACCCA ACTACTAGAT TTACAAAGCA ACTTCTTGTC AGCTCTGCAG 
TCTCCTATTG AGTATCAGAA GGAGCGTAGT GGCTCTCAAT CTCTGACCCG GAACTCTTCT 
GGGCGGATGG GTCAGTCCAA CAGCAGGCCC TGCTCCACGG TTTCACTCGG AGCAGAGAAA 
CCCTCTCTGT CTGGACGGAG CTCCACCCCG CTAAAGCTAT CTACCTCCAA TGGTGGTCCA 
TGCTCTGAAA GATACCGCAG GCAGTTTGAA GGCTTGGGCT GCAACGGTAC ATCGTCCTCC 
TTGATGGTGA CTCAAGATTA TACTGCACTG AAAGAGAATG AGATCTGTGT CACACAGGGT 
GAGGCCGTGC AGGTACTGGC CACTAACCAG CAGAACATGT ACCTTGTGTA CCGACCCGCA 
AATAGCCAAT CACCACCTGC AGAGGGCTGG GTGCCGGGAC ACATCCTAGG CCCCGTCACA 
AAACCTCTCA TAGACACTAC TGCAGACGCA AGCATCAAGA AATCTCTTTC GTGGAACACA 
CTTCGTGCTC GTAAGCGTGC AGAAAAGGAC AGCACGCCCC TCAAAAGTGA CTTTAAAGTT 
GAGAATGGAC TGCGTAAGCC AAAGGAAATT CTCAGCAGCA AAGTCAAAGT GAAAGAGTCC 
CGCAGTTCTG ATGAGTCTGA ATGTGAGGAT GAACTAGACC CCAAAACTAG TATGGAGTTA 
CTGAACCCTA ATTTCATCCA AGAGGTCGCT CCAGAGTTTC TGACGCCTCT AACAGACGTG 
ACGTGTGCTC TGGGAGAGAC GGTGGTTCTG TGCTGTAAAG TGTGTGCTCG ACCCAAACCC 
ACAATCACCT TGAAAGGACC TGATCAAAGT CTGCTGGCCA ACAATAACCG CTTCACTATT 
AATATCAGAG AAACCGGTGA TATAGTGTTG AAAATCTGCA ATCTAATGCC GCAGGACACT 
GGCATCTACA CGTGTGTTGC TGTGAATGAC CATGGTACAG CCTCCTCATC TGCATCCATT 
AAAGTACAAG GTATCCCAGC AGCCCCTGCT CGACCGGTGG CACAGGAGGC AAGCAGCACT 
GCAGTGATTG TCCACTGGCT TCCTCCAGCC AGCTCAGCAA ATTCTGCCAT CTCCAGCTAC 
ACTGTGGAAT ATAGGCAGGA AGATTCTCTG GTATGGCAGC AGTTGGTGGT CTCCACGGCA 
GATGTGTTTG TGAAGATTGA CAATCTGATT CCTGGTGGGC ACTACCAGTT CAGAGTCAGT 
GCCAGTAATC CCTGGGGCAC CAGCCCGCCT AGTGAACCCT CAAACATGGT GACATTATCC 
AGCACAGCAT CAACATATGA TGGCGCTGGA ATCCAGTGGA AAGACAACTT TGAATCTGCT 
TTTACAGAAT TATGTGAAAT TGGGCGGGGT CGTTTCTCTG TGGTGAGGAA GTGTCTCAGT 
AAAGCCACTA AGCAGGAGGT TGCTGTGAAG TTTGTGAATA AGAAGATGCA CAAGAAAGAA 
CAGGTGGCCC ATGAAGCTGA TATTCTACGC CATGTCCAGC ACCCCCAACT GGTGTCACTG 
ATTGATACAT ACGAGTCGCC TACAGCCTAC ATGCTGATTC TTGAACTTGT TGAGGATGGA 
CGTCTTCTTG ACTATCTGGT AGCTCATGAT GAGCTGATGG AGGAGAAAGT GGCATTCTTT 
ATTAAAGATA CACTGGAAGC TCTGCAGCAC TTGCACACCT GCAGAGTGGC TCATCTAGAC 
CTCAAGCCTG AAAACTTGTT GGTGGACCTC CACGTTCCGG TACCCTGCGT GAAGCTCTCA 
GATCTAGGTG ATGCTGTACA GGTTTCAAGT CACCGCTACG TACATCTCCT CCTGGGTAAC 
CCCGAGTTCG CTGCTCCAGA GCTCATCCAG GGCACTCCAG TGTCCCTCTC TACAGATGTG 
TGGAGTGTAG GGGTGCTGGC CTACGTCATG CTTAGTGGAG TCTCCCCATT CCTGGATGAG 
AGTCTGGAGG AGACCTGCGT CAACATCTGC AGGCTGGACT TCTGCTTTCC TCAAGAGTAC 
TTCTCCGATA TAAGCCAGGC AGCCAAAGAC TTCATTGTGT CCACGCTTAA CCAGGATCCC 
AGGAAGAGGC CAAGCTCGGC CTCGTGTCTT CAGCACCCAT GGGTCAGTGC TCATAGTGGA 
GATTACTCCA AAACACCTCT GGATACTGTC AGACTCGCTG CATTTATAGA CAGACGCAAA 
CAGCTACATG ATGTCAGGCC TGTCACAAAC ATTAAAGGAA TGGTCAGCAG CAGTATGGGA 
CACACCTTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kalrna ( XM_009304455.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302730.1 Danio rerio kalirin RhoGEF kinase a (kalrna), transcript variant X6, mRNA.

Price & Availability↑

CloneID	ODa48699
Clone ID Related Accession (Same CDS sequence)	XM_009304455.2 , XM_009304455.3
Accession Version	XM_009304455.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3852bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kalirin isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304455.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGTCATGA GGGGTGGTAA GAGTGTGTGC TCCTGCTGTG ACTGGATCTT CATTAAATGC 
TGCTGCTGCT GTCATGTTGG AGAAAACTAT ACAGTTAGTG GCCCAGAAGG AAAGACAGAG 
TCAGTGGCCA CACTGCAGCC ACAGCCATCC CTGAACTCTC TGCAGAGTAG CTCTCCTGGA 
CCCAAGCGCT CTGGGAATAC ACTGAAGAAA TGGCTGACCA GTCCTGTCCG ACGTCTAAGT 
CATGGCAGTA ACATCAAGAA GATCCCAAGT AAACAGAAAC AGAAACGTGA TGGTCGCAAG 
AGTATTGATA TGGGGCCGCC CGAACTACAG GATGACACCT TGGAAGAGCG AGTGCAAAAA 
GAAGAGGGCA CACTCTCCAA GTCTTCGTCT GGGATGCACA GTGGAGGAGA GGAAGAGCCT 
GAGGACGAGT CTCACACTCC TCTACCTCCA CCAATGGAGA TCATTAAAGA CCCGTCGGCT 
CAGGAAGAGA AGTCGTCTGT GCTTCTGGCT TCCCGGCAGA GCTCGGCTGA TGTCCCCAGT 
GCTGCTGAGC TTGTCAGTGC TATAGAAAAA CTGGTCAAAA CTAAAATGAC ACTGGAGCCA 
GGCTCATACC CGGGATTCAG CTCAGTCAAT CCAGCAGAGC AGAGCCCGAT TCCACCCAGA 
AACCCAGAAC TAGAGCAGAA AGCCAAAGCG CTACGAGGAC GAATGTTTGT AGTCAAGGAA 
TTAATCCAAA CGGAAAAAGA CTATGTTAAA GATCTGGGAA TTGTGGTTGA GGGGTTTATG 
AAGAGAATCG AGGAGAAAGG AGTTCCTGAT AACATGAAAG GAAAAGATAA GATCGTCTTC 
GGAAACATTC ACCAAATCTA TGACTGGCAC AAAGATTTTT TTGTTGGAGA ACTGGAGAAA 
TGCCTGGAAG ATCACGAAAA ACTGCCTGAA CTGTTCTGTA AACATGAGAG ACGGTTACAC 
ATGTATGTAG TTTACTGTCA GAACAAACCC AAATCCGAGT TCATCGTTGC AGAATATGAT 
TCATACTTTG AGGGGATTCA GCAAGACATT AACTCCAGAC TGAGCATCAG CGATTTTCTC 
ATTAAACCCA TTCAGAGAAT TACTAAATAC CAGCTTTTAT TAAAGGATGT TTTGAAGTAC 
AGTACAAAAG CAGGACTGGA TTGTCAGGAT ATAGAGAATG CAGTTGACCT GATGTCCCAG 
GTTCCCAAGC TTTGCAATGA CATGATGAAT CTGGGAAGAC TGCAGGGATA TGAGGGGAAG 
TTGACGAGTC AAGGAAAACT CTTGCAGCAG GAGACGTTCT TTGTGACCGA GCAGGACTCT 
GGCGTTCTGT CCCGCAGTAA AGAGCGCAGG GTTTTCCTGT TCGAACAAAT TGTCATCTTC 
AGCGAGCTGT TACGTAAAGG CTCTTCCACT CCAGGGTACC AGTTCAAGAA GAGCATAAAG 
GTGAGCTTCC TTGACTTGGA AGAGCATGTT GAAAATGATC CATGCAAGTT TGTGCTGTCG 
TGTCGTGGGT CGGCAGAACG TTTTACCCTT CAAGCAGCAA ACACGGACAT TAAACAAGTC 
TGGGTTCAGC ATATCACCCA ACTACTAGAT TTACAAAGCA ACTTCTTGTC AGCTCTGCAG 
TCTCCTATTG AGTATCAGAA GGAGCGTAGT GGCTCTCAAT CTCTGACCCG GAACTCTTCT 
GGGCGGATGG GTCAGTCCAA CAGCAGGCCC TGCTCCACGG TTTCACTCGG AGCAGAGAAA 
CCCTCTCTGT CTGGACGGAG CTCCACCCCG CTAAAGCTAT CTACCTCCAA TGGTGGTCCA 
TGCTCTGAAA GATACCGCAG GCAGTTTGAA GGCTTGGGCT GCAACGGTAC ATCGTCCTCC 
TTGATGGTGA CTCAAGATTA TACTGCACTG AAAGAGAATG AGATCTGTGT CACACAGGGT 
GAGGCCGTGC AGGTACTGGC CACTAACCAG CAGAACATGT ACCTTGTGTA CCGACCCGCA 
AATAGCCAAT CACCACCTGC AGAGGGCTGG GTGCCGGGAC ACATCCTAGG CCCCGTCACA 
AAACCTCTCA TAGACACTAC TGCAGACGCA AGCATCAAGA AATCTCTTTC GTGGAACACA 
CTTCGTGCTC GTAAGCGTGC AGAAAAGGAC AGCACGCCCC TCAAAAGTGA CTTTAAAGTT 
GAGAATGGAC TGCGTAAGCC AAAGGAAATT CTCAGCAGCA AAGTCAAAGT GAAAGAGTCC 
CGCAGTTCTG ATGAGTCTGA ATGTGAGGAT GAACTAGACC CCAAAACTAG TATGGAGTTA 
CTGAACCCTA ATTTCATCCA AGAGGTCGCT CCAGAGTTTC TGACGCCTCT AACAGACGTG 
ACGTGTGCTC TGGGAGAGAC GGTGGTTCTG TGCTGTAAAG TGTGTGCTCG ACCCAAACCC 
ACAATCACCT TGAAAGGACC TGATCAAAGT CTGCTGGCCA ACAATAACCG CTTCACTATT 
AATATCAGAG AAACCGGTGA TATAGTGTTG AAAATCTGCA ATCTAATGCC GCAGGACACT 
GGCATCTACA CGTGTGTTGC TGTGAATGAC CATGGTACAG CCTCCTCATC TGCATCCATT 
AAAGTACAAG GTATCCCAGC AGCCCCTGCT CGACCGGTGG CACAGGAGGC AAGCAGCACT 
GCAGTGATTG TCCACTGGCT TCCTCCAGCC AGCTCAGCAA ATTCTGCCAT CTCCAGCTAC 
ACTGTGGAAT ATAGGCAGGA AGATTCTCTG GTATGGCAGC AGTTGGTGGT CTCCACGGCA 
GATGTGTTTG TGAAGATTGA CAATCTGATT CCTGGTGGGC ACTACCAGTT CAGAGTCAGT 
GCCAGTAATC CCTGGGGCAC CAGCCCGCCT AGTGAACCCT CAAACATGGT GACATTATCC 
AGCACAGCAT CAACATATGA TGGCGCTGGA ATCCAGTGGA AAGACAACTT TGAATCTGCT 
TTTACAGAAT TATGTGAAAT TGGGCGGGGT CGTTTCTCTG TGGTGAGGAA GTGTCTCAGT 
AAAGCCACTA AGCAGGAGGT TGCTGTGAAG TTTGTGAATA AGAAGATGCA CAAGAAAGAA 
CAGGTGGCCC ATGAAGCTGA TATTCTACGC CATGTCCAGC ACCCCCAACT GGTGTCACTG 
ATTGATACAT ACGAGTCGCC TACAGCCTAC ATGCTGATTC TTGAACTTGT TGAGGATGGA 
CGTCTTCTTG ACTATCTGGT AGCTCATGAT GAGCTGATGG AGGAGAAAGT GGCATTCTTT 
ATTAAAGATA CACTGGAAGC TCTGCAGCAC TTGCACACCT GCAGAGTGGC TCATCTAGAC 
CTCAAGCCTG AAAACTTGTT GGTGGACCTC CACGTTCCGG TACCCTGCGT GAAGCTCTCA 
GATCTAGGTG ATGCTGTACA GGTTTCAAGT CACCGCTACG TACATCTCCT CCTGGGTAAC 
CCCGAGTTCG CTGCTCCAGA GCTCATCCAG GGCACTCCAG TGTCCCTCTC TACAGATGTG 
TGGAGTGTAG GGGTGCTGGC CTACGTCATG CTTAGTGGAG TCTCCCCATT CCTGGATGAG 
AGTCTGGAGG AGACCTGCGT CAACATCTGC AGGCTGGACT TCTGCTTTCC TCAAGAGTAC 
TTCTCCGATA TAAGCCAGGC AGCCAAAGAC TTCATTGTGT CCACGCTTAA CCAGGATCCC 
AGGAAGAGGC CAAGCTCGGC CTCGTGTCTT CAGCACCCAT GGGTCAGTGC TCATAGTGGA 
GATTACTCCA AAACACCTCT GGATACTGTC AGACTCGCTG CATTTATAGA CAGACGCAAA 
CAGCTACATG ATGTCAGGCC TGTCACAAAC ATTAAAGGAA TGGTCAGCAG CAGTATGGGA 
CACACCTTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302730.1
CDS	57..3908
Translation	MVMRGGKSVCSCCDWIFIKCCCCCHVGENYTVSGPEGKTESVATLQPQPSLNSLQSSSPGPKRSGNTLKKWLTSPVRRLSHGSNIKKIPSKQKQKRDGRKSIDMGPPELQDDTLEERVQKEEGTLSKSSSGMHSGGEEEPEDESHTPLPPPMEIIKDPSAQEEKSSVLLASRQSSADVPSAAELVSAIEKLVKTKMTLEPGSYPGFSSVNPAEQSPIPPRNPELEQKAKALRGRMFVVKELIQTEKDYVKDLGIVVEGFMKRIEEKGVPDNMKGKDKIVFGNIHQIYDWHKDFFVGELEKCLEDHEKLPELFCKHERRLHMYVVYCQNKPKSEFIVAEYDSYFEGIQQDINSRLSISDFLIKPIQRITKYQLLLKDVLKYSTKAGLDCQDIENAVDLMSQVPKLCNDMMNLGRLQGYEGKLTSQGKLLQQETFFVTEQDSGVLSRSKERRVFLFEQIVIFSELLRKGSSTPGYQFKKSIKVSFLDLEEHVENDPCKFVLSCRGSAERFTLQAANTDIKQVWVQHITQLLDLQSNFLSALQSPIEYQKERSGSQSLTRNSSGRMGQSNSRPCSTVSLGAEKPSLSGRSSTPLKLSTSNGGPCSERYRRQFEGLGCNGTSSSLMVTQDYTALKENEICVTQGEAVQVLATNQQNMYLVYRPANSQSPPAEGWVPGHILGPVTKPLIDTTADASIKKSLSWNTLRARKRAEKDSTPLKSDFKVENGLRKPKEILSSKVKVKESRSSDESECEDELDPKTSMELLNPNFIQEVAPEFLTPLTDVTCALGETVVLCCKVCARPKPTITLKGPDQSLLANNNRFTINIRETGDIVLKICNLMPQDTGIYTCVAVNDHGTASSSASIKVQGIPAAPARPVAQEASSTAVIVHWLPPASSANSAISSYTVEYRQEDSLVWQQLVVSTADVFVKIDNLIPGGHYQFRVSASNPWGTSPPSEPSNMVTLSSTASTYDGAGIQWKDNFESAFTELCEIGRGRFSVVRKCLSKATKQEVAVKFVNKKMHKKEQVAHEADILRHVQHPQLVSLIDTYESPTAYMLILELVEDGRLLDYLVAHDELMEEKVAFFIKDTLEALQHLHTCRVAHLDLKPENLLVDLHVPVPCVKLSDLGDAVQVSSHRYVHLLLGNPEFAAPELIQGTPVSLSTDVWSVGVLAYVMLSGVSPFLDESLEETCVNICRLDFCFPQEYFSDISQAAKDFIVSTLNQDPRKRPSSASCLQHPWVSAHSGDYSKTPLDTVRLAAFIDRRKQLHDVRPVTNIKGMVSSSMGHTL

Target ORF information:

RefSeq Version	XM_009304455.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kalirin RhoGEF kinase a (kalrna), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304455.3

ORF Insert Sequence:

ATGGTCATGA GGGGTGGTAA GAGTGTGTGC TCCTGCTGTG ACTGGATCTT CATTAAATGC 
TGCTGCTGCT GTCATGTTGG AGAAAACTAT ACAGTTAGTG GCCCAGAAGG AAAGACAGAG 
TCAGTGGCCA CACTGCAGCC ACAGCCATCC CTGAACTCTC TGCAGAGTAG CTCTCCTGGA 
CCCAAGCGCT CTGGGAATAC ACTGAAGAAA TGGCTGACCA GTCCTGTCCG ACGTCTAAGT 
CATGGCAGTA ACATCAAGAA GATCCCAAGT AAACAGAAAC AGAAACGTGA TGGTCGCAAG 
AGTATTGATA TGGGGCCGCC CGAACTACAG GATGACACCT TGGAAGAGCG AGTGCAAAAA 
GAAGAGGGCA CACTCTCCAA GTCTTCGTCT GGGATGCACA GTGGAGGAGA GGAAGAGCCT 
GAGGACGAGT CTCACACTCC TCTACCTCCA CCAATGGAGA TCATTAAAGA CCCGTCGGCT 
CAGGAAGAGA AGTCGTCTGT GCTTCTGGCT TCCCGGCAGA GCTCGGCTGA TGTCCCCAGT 
GCTGCTGAGC TTGTCAGTGC TATAGAAAAA CTGGTCAAAA CTAAAATGAC ACTGGAGCCA 
GGCTCATACC CGGGATTCAG CTCAGTCAAT CCAGCAGAGC AGAGCCCGAT TCCACCCAGA 
AACCCAGAAC TAGAGCAGAA AGCCAAAGCG CTACGAGGAC GAATGTTTGT AGTCAAGGAA 
TTAATCCAAA CGGAAAAAGA CTATGTTAAA GATCTGGGAA TTGTGGTTGA GGGGTTTATG 
AAGAGAATCG AGGAGAAAGG AGTTCCTGAT AACATGAAAG GAAAAGATAA GATCGTCTTC 
GGAAACATTC ACCAAATCTA TGACTGGCAC AAAGATTTTT TTGTTGGAGA ACTGGAGAAA 
TGCCTGGAAG ATCACGAAAA ACTGCCTGAA CTGTTCTGTA AACATGAGAG ACGGTTACAC 
ATGTATGTAG TTTACTGTCA GAACAAACCC AAATCCGAGT TCATCGTTGC AGAATATGAT 
TCATACTTTG AGGGGATTCA GCAAGACATT AACTCCAGAC TGAGCATCAG CGATTTTCTC 
ATTAAACCCA TTCAGAGAAT TACTAAATAC CAGCTTTTAT TAAAGGATGT TTTGAAGTAC 
AGTACAAAAG CAGGACTGGA TTGTCAGGAT ATAGAGAATG CAGTTGACCT GATGTCCCAG 
GTTCCCAAGC TTTGCAATGA CATGATGAAT CTGGGAAGAC TGCAGGGATA TGAGGGGAAG 
TTGACGAGTC AAGGAAAACT CTTGCAGCAG GAGACGTTCT TTGTGACCGA GCAGGACTCT 
GGCGTTCTGT CCCGCAGTAA AGAGCGCAGG GTTTTCCTGT TCGAACAAAT TGTCATCTTC 
AGCGAGCTGT TACGTAAAGG CTCTTCCACT CCAGGGTACC AGTTCAAGAA GAGCATAAAG 
GTGAGCTTCC TTGACTTGGA AGAGCATGTT GAAAATGATC CATGCAAGTT TGTGCTGTCG 
TGTCGTGGGT CGGCAGAACG TTTTACCCTT CAAGCAGCAA ACACGGACAT TAAACAAGTC 
TGGGTTCAGC ATATCACCCA ACTACTAGAT TTACAAAGCA ACTTCTTGTC AGCTCTGCAG 
TCTCCTATTG AGTATCAGAA GGAGCGTAGT GGCTCTCAAT CTCTGACCCG GAACTCTTCT 
GGGCGGATGG GTCAGTCCAA CAGCAGGCCC TGCTCCACGG TTTCACTCGG AGCAGAGAAA 
CCCTCTCTGT CTGGACGGAG CTCCACCCCG CTAAAGCTAT CTACCTCCAA TGGTGGTCCA 
TGCTCTGAAA GATACCGCAG GCAGTTTGAA GGCTTGGGCT GCAACGGTAC ATCGTCCTCC 
TTGATGGTGA CTCAAGATTA TACTGCACTG AAAGAGAATG AGATCTGTGT CACACAGGGT 
GAGGCCGTGC AGGTACTGGC CACTAACCAG CAGAACATGT ACCTTGTGTA CCGACCCGCA 
AATAGCCAAT CACCACCTGC AGAGGGCTGG GTGCCGGGAC ACATCCTAGG CCCCGTCACA 
AAACCTCTCA TAGACACTAC TGCAGACGCA AGCATCAAGA AATCTCTTTC GTGGAACACA 
CTTCGTGCTC GTAAGCGTGC AGAAAAGGAC AGCACGCCCC TCAAAAGTGA CTTTAAAGTT 
GAGAATGGAC TGCGTAAGCC AAAGGAAATT CTCAGCAGCA AAGTCAAAGT GAAAGAGTCC 
CGCAGTTCTG ATGAGTCTGA ATGTGAGGAT GAACTAGACC CCAAAACTAG TATGGAGTTA 
CTGAACCCTA ATTTCATCCA AGAGGTCGCT CCAGAGTTTC TGACGCCTCT AACAGACGTG 
ACGTGTGCTC TGGGAGAGAC GGTGGTTCTG TGCTGTAAAG TGTGTGCTCG ACCCAAACCC 
ACAATCACCT TGAAAGGACC TGATCAAAGT CTGCTGGCCA ACAATAACCG CTTCACTATT 
AATATCAGAG AAACCGGTGA TATAGTGTTG AAAATCTGCA ATCTAATGCC GCAGGACACT 
GGCATCTACA CGTGTGTTGC TGTGAATGAC CATGGTACAG CCTCCTCATC TGCATCCATT 
AAAGTACAAG GTATCCCAGC AGCCCCTGCT CGACCGGTGG CACAGGAGGC AAGCAGCACT 
GCAGTGATTG TCCACTGGCT TCCTCCAGCC AGCTCAGCAA ATTCTGCCAT CTCCAGCTAC 
ACTGTGGAAT ATAGGCAGGA AGATTCTCTG GTATGGCAGC AGTTGGTGGT CTCCACGGCA 
GATGTGTTTG TGAAGATTGA CAATCTGATT CCTGGTGGGC ACTACCAGTT CAGAGTCAGT 
GCCAGTAATC CCTGGGGCAC CAGCCCGCCT AGTGAACCCT CAAACATGGT GACATTATCC 
AGCACAGCAT CAACATATGA TGGCGCTGGA ATCCAGTGGA AAGACAACTT TGAATCTGCT 
TTTACAGAAT TATGTGAAAT TGGGCGGGGT CGTTTCTCTG TGGTGAGGAA GTGTCTCAGT 
AAAGCCACTA AGCAGGAGGT TGCTGTGAAG TTTGTGAATA AGAAGATGCA CAAGAAAGAA 
CAGGTGGCCC ATGAAGCTGA TATTCTACGC CATGTCCAGC ACCCCCAACT GGTGTCACTG 
ATTGATACAT ACGAGTCGCC TACAGCCTAC ATGCTGATTC TTGAACTTGT TGAGGATGGA 
CGTCTTCTTG ACTATCTGGT AGCTCATGAT GAGCTGATGG AGGAGAAAGT GGCATTCTTT 
ATTAAAGATA CACTGGAAGC TCTGCAGCAC TTGCACACCT GCAGAGTGGC TCATCTAGAC 
CTCAAGCCTG AAAACTTGTT GGTGGACCTC CACGTTCCGG TACCCTGCGT GAAGCTCTCA 
GATCTAGGTG ATGCTGTACA GGTTTCAAGT CACCGCTACG TACATCTCCT CCTGGGTAAC 
CCCGAGTTCG CTGCTCCAGA GCTCATCCAG GGCACTCCAG TGTCCCTCTC TACAGATGTG 
TGGAGTGTAG GGGTGCTGGC CTACGTCATG CTTAGTGGAG TCTCCCCATT CCTGGATGAG 
AGTCTGGAGG AGACCTGCGT CAACATCTGC AGGCTGGACT TCTGCTTTCC TCAAGAGTAC 
TTCTCCGATA TAAGCCAGGC AGCCAAAGAC TTCATTGTGT CCACGCTTAA CCAGGATCCC 
AGGAAGAGGC CAAGCTCGGC CTCGTGTCTT CAGCACCCAT GGGTCAGTGC TCATAGTGGA 
GATTACTCCA AAACACCTCT GGATACTGTC AGACTCGCTG CATTTATAGA CAGACGCAAA 
CAGCTACATG ATGTCAGGCC TGTCACAAAC ATTAAAGGAA TGGTCAGCAG CAGTATGGGA 
CACACCTTAT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kalrna ( XM_021478826.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021334501.1 Danio rerio kalirin RhoGEF kinase a (kalrna), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa144040
Clone ID Related Accession (Same CDS sequence)	XM_021478826.1
Accession Version	XM_021478826.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8901bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kalirin isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGTATTTAT GGAGATACGG ATGTGGCATG CTGTCTGCCT CTCTCATCAG AAGAAAGACA 
GGGTCTTTCA GAAGTGAAGG AATTAAAGCC TCTGATGTCC TTCCTGTGCT GAGAGAAAAA 
ATAGCATTTG TGTCAGGCGG TCGTGATAAG CGAGGTGGCC CTATACTGAC CTTTCCTGCA 
AGGAGTAACC ATGACCGAAT AAAACAAGAG GACCTGAGAC GGCTCGTAAC ATACCTGTCC 
ACTGTGCCCA GTGAGGACGT TTGTAAGAGA GGCTTCACTG TGATTATTGA TATGCGTGGC 
TCTAAATGGG AGCTAATAAA GCCACTGCTG AAGACGCTAC AGGAAGCATT TCCAGCTGAG 
ATCTGCGTCG CTCTCATCAT CAAACCAGAC AACTTCTGGC AGAAGCAGAA GACTAACTTT 
GGTAGCGCCA AGTTCACCTT TGAGACTAGC ATGGTATCAG TGGAGGGCTT GACCAAACTA 
GTGGACCCAT CCCAGCTAAC GGACGACTTT GAAGGTTCTC TGGAATACAA TCATGAGGAA 
TGGCTTGAGC TCCGGGTGGC TCTGGAGGAG TTCCTGGCCA GCGCCGTTCA CCTCCTCTCT 
CGCCTGGAAG ATCTACAGGA GACTCTGGCT AAGAAGGAGT TCCCGGTGGA TGTGGAAGCC 
TCTCGTAGAC TCATCGATGA GCACACACAG CTGAAGAAGA AGGTGATGAA GGCTCCGGTG 
GAAGAGCTGG ACCACGAAGG CCAGAGACTC TTGCAGTGCA TCCGCTCCAG CGACGGCTTC 
TCAGGGAGGA ACTGCATTTC TGGAAGTGCC GACTTCCAGA GCGTGGTTCC GAAGATCGCC 
AGTCTGCTGG ATAAACTCCA CAGCACTCGA CAGCACTTGC ACCAGATGTG GCACCTACGA 
AAACTCAAGC TGGATCAGTG CTTTCAGCTC CGACTGTTTG AACAGGACGC AGAGAAGATG 
TTTGACTGGA TCAGTCACAA TAAGGAATTG TTTCTTCAGA GCCACACGGA GATCGGTGTG 
AGCTACCAGC ATGCTGTGGA GCTACAGACG CAACACAACC ACTTTGCCAT GAACTCAATG 
AATGCCTATG TGAACATAAA CCGCATCATG TCGGTGGCGT CGCGGCTGTC GGAGGCGGGC 
CACTACGCCG CTCAGCAGAT CAAACAGATC AGCACTCAGC TGGATCAGGA GTGGAAGAGC 
TTCGCGGCTG CTCTCGATGA ACGCAGCACC ATCCTTGCCA TGTCTGCCGT CTTCCATCAG 
AAATCTGAAC AGTTTTTGTC CGGTGTGGAA ACATGGTGTA AGATGTGCAG TGAAGGCGGC 
TTGCCATCAG AGATGCAGGA TCTGGAATCG GCCATTCATC ACCATCAGAC GCTCTATGAA 
CAAGTCACAC AAGCATACAC CGAGGTGAGT CAGGACGGTA AGGCTTTGCT GGACGTTCTT 
CAGCGGCCGT TGAGTCCTGG GAACGCAGAG TCACTGACTG CGACAGCGAA CTACTCTAAA 
GCCGTGCACT GTATACTGGA CGTGGTCCAC GAGGTCCTGC ATCATCAGAG ACGTCTGGAG 
AACATCTGGC AGCACCGTAA AGTCCGTCTG CATCAGAGAC TGCAGCTCTG TGTCTTCCAG 
CAGGACGTCC AACAGGTGAT TGACTGGATA GAGAATCACG GGGAGGCTTT CCTCAGCAAA 
CACACTGGTG TGGGCAAATC CCTGCACAGA GCCAGAGCCC TCCAGAAACG CCATGATGAC 
TTTGAGGAGG TGGCACAGAA TACATACACT AATGCAGATA AGCTGCTGGA AGCTGCTGAG 
CAGTTGGCTC AGACGGGCGA GTGTGACCCT GAGGAGATCT ACAAGGCAGC GCGACACCTG 
GAAGTCAGGA TCCAGGACTT TGTTAGGCGA GTGGAGCAAA GAAAACTACT GCTGGACATG 
TCTGTCTCTT TCCACACGCA CACTAAAGAG TTGTGGACGT GGATGGAGGA CCTGCAGAAA 
GAGCTGCTGG AGGAAGTTTG TTCTGACTCA GTCGATTCAG TTCAGGAGCT GATAAAACAG 
TTTCAGCAGC AGCAGACGGC CACGCTGGAG GCAACACTCA ATGTCATTAA AGAGGGAGAA 
GATCTTATGC AACAACTCCG AGATTCAGCA ATGAGCAGTA ATAAGATGCC CCACACCAGC 
TCCATCGCTC ACATTGAGGG CGTCCTGCAG CAGCTAGAGG AGGCGCAGGG CCACATGGAG 
GAACTCTTCC ATGAGAGAAA GATCAAACTC GACATCTTCC TGCAACTGCG CATCTTTGAG 
CAGTACACCA TTGAGGTAAC GGCAGAGTTA GACGCGTGGA ATGAAGACTT GGTTCGTCAG 
ATGAATGAGT ATACTGGAGA AGAACCCAGT CTGGCTGAGC AGAGACTCCA GCGGCACGCA 
GAGAGGAAAC TGGCCATGAC TAACATGACC TACGAAGTTA TGCAGCAGGG TCAGGACCTG 
CATCAGTACA TCATGGAAGT CCAAGCTTCA GGTATTGAGT TGACCGGAGA GAAGGAGGTG 
GATCTGGCAA CACAAGTTCA GGAGCTGCTG GAGTTCCTTC ATGAGAAACA GCACGAGTTG 
GACATGAGCG CCGAACACAC ACAGAAGCGC CTGGAGCAGT GTGTGCAGCT CCGCCACCTG 
CAGGCTGAGG TTAAACAGGT TCTGGGGTGG ATTCGTAATG GTGAATCTAT GTTGGATGCC 
AGTATGGTGA ATGCAAGTTC ACTGTCAGAG GCCGAACAAC TACAGAGAGA ACATGAACAG 
TTCCAGCTGG CCATAGAGTC TTTGTTCCAC GCTAACTCTC TCCAGAAAAC CCATCAGAGT 
GCACTACAGG TCCAGCAGAA GGCAGAAATC ATGTTACAGG CTGGACACTT TGACCCCGAG 
GCCATCCGCA GCTGTGCAGA GAAGGTGGCC GTGCACTGGC AGCAGCTCAT GCTCAAGATG 
GAGGACAGAC TCAAACTGGT TAATGCCTCG GTGGCCTTCT ACAAGACGTC AGAACAGGTG 
TGTAGTGTCC TGGAGAGTCT GGAGCAGGAG TACCGCAGAG ATGAAGACTG GTGTGGAGCT 
CATGATAAAC TAGGATCTTC ATCAGAGACT GATCACGTTC TGCCTCTCAT CAGCAAACAC 
CTGGAGCAGA AAGAAGCTTT TCTCAAGGCA TGCACTCTGG CCAGGCGCAA TGCTGAGGTT 
TTTCTGAAAT ACATCCACCG GAACAATGTC AGCTCTCAGG GATCATCTAG CAACACTCGT 
GGCCCTGAGC AACAGGTCAA AGCCATCCTG AATGAACTGC TGCAGAGGGA AAACAGAGTT 
TTGCATTTCT GGACCATGAA GAAGCGGCGT TTGGATCAGT GTCAGCAGTA TGTGGTGTTT 
GAACGCAATG CCAAACAGGC ATTAGAGTGG ATTGAGGAGG CAGGAGAGGT CTATCTGGCC 
ACTCACACCT GCATCGGAGA CTCTATGGAG AAAACTCAGG GACTCCTCAA AGAATACGAA 
GAATTCTGCA TCTCCGCAAA GCAAACTAAG GAGAAAGTGA AGATGCTGAT CCAGCTGGCG 
GATAACTTTG TTGAGAAAGG ACACATGCAT GTCACCGACT TGAAGAAGTG TCTGACGGCA 
GTGGACAAGC GCTACAGGGA CTTCTCCATG CGCATGGGCA AATACCGCTG TGACCTGGAA 
AATGTGCTGG GCATCAGCTC TGAGGATAAT AAGGATCTGG AGCTGGATAT CATCCCAGCC 
AGTCTGACTG GCCCAGAGGT TAAACTCCGT GACCCCAACC ACGAGGTGAA TGAAGAAAAG 
AGGAAATCAG CCCGGAAGAA AGAGTTTATT ATAGCAGAGC TGTTGCAGAC AGAAAAGGCT 
TATGTGAGGG ACTTACATGA ATGCTTAGAG ACATATTTGT GGGAGATGAC CAATGGTGTG 
GAGGAAATTC CTCCAGGAAT CGCCAACAAA GAGCACATCA TCTTTGGCAA CATTCAGGAG 
ATTTACGACT TCCACAATAA TATATTCCTG AAGGAGCTGA TCAATTATGA GCAACTGCCT 
GAGGATGTGG GACATTGCTT TGTGACATGG GCCGATAAGT TTAACATTTA TGTGACATAC 
TGCAAAAACA AACCAGATTC CAGTCAGCTC ATCCTAGACC ATGCTGGGAG TTTTTTTGAT 
GAGATTCAGA AGAGACACAG TCTGACCAAC TCTATATCAT CATACCTCAT CAAACCTGTA 
CAGAGGATCA CCAAATATCA GCTGCTGCTC AAGGAGCTGC TGTCCTGCTG TGAAGAAGGC 
AAAGGTGAAA TTAAAGATGG CCTGGAGGTC ATGCTGAGTG TGCCAAAGAG AGCCAATGAC 
GCCATGCACG TCAGCATGCT GGAGGGTTTT GATGCAAATT TGGATGTTCA GGGTGAACTC 
ATCCTCCAAG ACACCTTCCA GGTGTGGGAC CCCAAATCAC TAATCCGGAA GGGGCGAGAC 
CGCCACCTCT TTCTCTTCGA GATCTCCCTG GTCTTCAGTA AAGAGATCAA AGACTCAGCA 
GGGCGATCCA AATACATCTT TAAAAACAAA CTACTGACTT CTGAATTGGG GGTGACAGAG 
CATGTAGAGG GGGACCCGTG CAAATTTGCT CTGTGGGTGG GACGCACACC ATCGTCTGAC 
AACAAGACTG TCCTCAAGGC ATCTAGTATT GAGGTGAAGC AAGAATGGAT TAAAAACATC 
CGAGAGGTCA TTCAGGAGAG AATAATTCAC CTGAAGGGGG CGCTAAAGGA ACCTATCCAC 
ATTCCCAAAA CTCCAGCCAA ACCTCGCAAT AACAGCAAGA GGGACGCTGG TGAGGATGCT 
GACAGCCAGG GAGACGGCAG CAGTCAGCCG GACACCATCT CCATAGCATC CAGAACCTCT 
CAGAACACTG TAGACAGTGA CAAGCTCTCT GGAGGGTGTG AGCTGACGGT GGTCCTGCAG 
GACTTTACAG CAAGCAGCTC ATCAGAGCTC AGCATCCAGA CGGGTCAGAC AGTGGAGCTG 
CTGGAGCGGC CCAGTGACCG TCCAGGATGG TGTCTGGTAC GGACCACCGA AAAGACGCCT 
CCTCAAGAGG GACTGGTGCC CAGCAGTGCT CTGTGTATCT CGCACTCACG CAGCAGTGTG 
GAGATGGACT GCTTTTTCCC AGCAGGCAAA GAAAACTATA CAGTTAGTGG CCCAGAAGGA 
AAGACAGAGT CAGTGGCCAC ACTGCAGCCA CAGCCATCCC TGAACTCTCT GCAGAGTAGC 
TCTCCTGGAC CCAAGCGCTC TGGGAATACA CTGAAGAAAT GGCTGACCAG TCCTGTCCGA 
CGTCTAAGTC ATGGCAGTAA CATCAAGAAG ATCCCAAGTA AACAGAAACA GAAACGTGAT 
GGTCGCAAGA GTATTGATAT GGGGCCGCCC GAACTACAGG ATGACACCTT GGAAGAGCGA 
GTGCAAAAAG AAGAGGGCAC ACTCTCCAAG TCTTCGTCTG GGATGCACAG TGGAGGAGAG 
GAAGAGCCTG AGGACGAGTC TCACACTCCT CTACCTCCAC CAATGGAGAT CATTAAAGAC 
CCGTCGGCTC AGGAAGAGAA GTCGTCTGTG CTTCTGGCTT CCCGGCAGAG CTCGGCTGAT 
GTCCCCAGTG CTGCTGAGCT TGTCAGTGCT ATAGAAAAAC TGGTCAAAAC TAAAATGACA 
CTGGAGCCAG GCTCATACCC GGGATTCAGC TCAGTCAATC CAGCAGAGCA GAGCCCGATT 
CCACCCAGAA ACCCAGAACT AGAGCAGAAA GCCAAAGCGC TACGAGGACG AATGTTTGTA 
GTCAAGGAAT TAATCCAAAC GGAAAAAGAC TATGTTAAAG ATCTGGGAAT TGTGGTTGAG 
GGGTTTATGA AGAGAATCGA GGAGAAAGGA GTTCCTGATA ACATGAAAGG AAAAGATAAG 
ATCGTCTTCG GAAACATTCA CCAAATCTAT GACTGGCACA AAGATTTTTT TGTTGGAGAA 
CTGGAGAAAT GCCTGGAAGA TCACGAAAAA CTGCCTGAAC TGTTCTGTAA ACATGAGAGA 
CGGTTACACA TGTATGTAGT TTACTGTCAG AACAAACCCA AATCCGAGTT CATCGTTGCA 
GAATATGATT CATACTTTGA GGGGATTCAG CAAGACATTA ACTCCAGACT GAGCATCAGC 
GATTTTCTCA TTAAACCCAT TCAGAGAATT ACTAAATACC AGCTTTTATT AAAGGATGTT 
TTGAAGTACA GTACAAAAGC AGGACTGGAT TGTCAGGATA TAGAGAATGC AGTTGACCTG 
ATGTCCCAGG TTCCCAAGCT TTGCAATGAC ATGATGAATC TGGGAAGACT GCAGGGATAT 
GAGGGGAAGT TGACGAGTCA AGGAAAACTC TTGCAGCAGG AGACGTTCTT TGTGACCGAG 
CAGGACTCTG GCGTTCTGTC CCGCAGTAAA GAGCGCAGGG TTTTCCTGTT CGAACAAATT 
GTCATCTTCA GCGAGCTGTT ACGTAAAGGC TCTTCCACTC CAGGGTACCA GTTCAAGAAG 
AGCATAAAGG TGAGCTTCCT TGACTTGGAA GAGCATGTTG AAAATGATCC ATGCAAGTTT 
GTGCTGTCGT GTCGTGGGTC GGCAGAACGT TTTACCCTTC AAGCAGCAAA CACGGACATT 
AAACAAGTCT GGGTTCAGCA TATCACCCAA CTACTAGATT TACAAAGCAA CTTCTTGTCA 
GCTCTGCAGT CTCCTATTGA GTATCAGAAG GAGCGTAGTG GCTCTCAATC TCTGACCCGG 
AACTCTTCTG GGCGGATGGG TCAGTCCAAC AGCAGGCCCT GCTCCACGGT TTCACTCGGA 
GCAGAGAAAC CCTCTCTGTC TGGACGGAGC TCCACCCCGC TAAAGCTATC TACCTCCAAT 
GGTGGTCCAT GCTCTGAAAG ATACCGCAGG CAGTTTGAAG GCTTGGGCTG CAACGGTACA 
TCGTCCTCCT TGATGGTGAC TCAAGATTAT ACTGCACTGA AAGAGAATGA GATCTGTGTC 
ACACAGGGTG AGGCCGTGCA GGTACTGGCC ACTAACCAGC AGAACATGTA CCTTGTGTAC 
CGACCCGCAA ATAGCCAATC ACCACCTGCA GAGGGCTGGG TGCCGGGACA CATCCTAGGC 
CCCGTCACAA AACCTCTCAT AGACACTACT GCAGACGCAA GCATCAAGAA ATCTCTTTCG 
TGGAACACAC TTCGTGCTCG TAAGCGTGCA GAAAAGGACA GCACGCCCCT CAAAAGTGAC 
TTTAAAGTTG AGAATGGACT GCGTAAGCCA AAGGAAATTC TCAGCAGCAA AGTCAAAGTG 
AAAGAGTCCC GCAGTTCTGA TGAGTCTGAA TGTGAGGATG AACTAGACCC CAAAACTAGT 
ATGGAGTTAC TGAACCCTAA TTTCATCCAA GAGGTCGCTC CAGAGTTTCT GACGCCTCTA 
ACAGACGTGA CGTGTGCTCT GGGAGAGACG GTGGTTCTGT GCTGTAAAGT GTGTGCTCGA 
CCCAAACCCA CAATCACCTT GAAAGGACCT GATCAAAGTC TGCTGGCCAA CAATAACCGC 
TTCACTATTA ATATCAGAGA AACCGGTGAT ATAGTGTTGA AAATCTGCAA TCTAATGCCG 
CAGGACACTG GCATCTACAC GTGTGTTGCT GTGAATGACC ATGGTACAGC CTCCTCATCT 
GCATCCATTA AAGTACAAGG TATCCCAGCA GCCCCTGCTC GACCGGTGGC ACAGGAGGCA 
AGCAGCACTG CAGTGATTGT CCACTGGCTT CCTCCAGCCA GCTCAGCAAA TTCTGCCATC 
TCCAGCTACA CTGTGGAATA TAGGCAGGAA GATTCTCTGG TATGGCAGCA GTTGGTGGTC 
TCCACGGCAG ATGTGTTTGT GAAGATTGAC AATCTGATTC CTGGTGGGCA CTACCAGTTC 
AGAGTCAGTG CCAGTAATCC CTGGGGCACC AGCCCGCCTA GTGAACCCTC AAACATGGTG 
ACATTATCCA GCACAGCATC AACATATGAT GGCGCTGGAA TCCAGTGGAA AGACAACTTT 
GAATCTGCTT TTACAGAATT ATGTGAAATT GGGCGGGGTC GTTTCTCTGT GGTGAGGAAG 
TGTCTCAGTA AAGCCACTAA GCAGGAGGTT GCTGTGAAGT TTGTGAATAA GAAGATGCAC 
AAGAAAGAAC AGGTGGCCCA TGAAGCTGAT ATTCTACGCC ATGTCCAGCA CCCCCAACTG 
GTGTCACTGA TTGATACATA CGAGTCGCCT ACAGCCTACA TGCTGATTCT TGAACTTGTT 
GAGGATGGAC GTCTTCTTGA CTATCTGGTA GCTCATGATG AGCTGATGGA GGAGAAAGTG 
GCATTCTTTA TTAAAGATAC ACTGGAAGCT CTGCAGCACT TGCACACCTG CAGAGTGGCT 
CATCTAGACC TCAAGCCTGA AAACTTGTTG GTGGACCTCC ACGTTCCGGT ACCCTGCGTG 
AAGCTCTCAG ATCTAGGTGA TGCTGTACAG GTTTCAAGTC ACCGCTACGT ACATCTCCTC 
CTGGGTAACC CCGAGTTCGC TGCTCCAGAG CTCATCCAGG GCACTCCAGT GTCCCTCTCT 
ACAGATGTGT GGAGTGTAGG GGTGCTGGCC TACGTCATGC TTAGTGGAGT CTCCCCATTC 
CTGGATGAGA GTCTGGAGGA GACCTGCGTC AACATCTGCA GGCTGGACTT CTGCTTTCCT 
CAAGAGTACT TCTCCGATAT AAGCCAGGCA GCCAAAGACT TCATTGTGTC CACGCTTAAC 
CAGGATCCCA GGAAGAGGCC AAGCTCGGCC TCGTGTCTTC AGCACCCATG GGTCAGTGCT 
CATAGTGGAG ATTACTCCAA AACACCTCTG GATACTGTCA GACTCGCTGC ATTTATAGAC 
AGACGCAAAC AGCTACATGA TGTCAGGCCT GTCACAAACA TTAAAGGAAT GGTCAGCAGC 
AGTATGGGAC ACACCTTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021334501.1
CDS	120..9020
Translation	MYLWRYGCGMLSASLIRRKTGSFRSEGIKASDVLPVLREKIAFVSGGRDKRGGPILTFPARSNHDRIKQEDLRRLVTYLSTVPSEDVCKRGFTVIIDMRGSKWELIKPLLKTLQEAFPAEICVALIIKPDNFWQKQKTNFGSAKFTFETSMVSVEGLTKLVDPSQLTDDFEGSLEYNHEEWLELRVALEEFLASAVHLLSRLEDLQETLAKKEFPVDVEASRRLIDEHTQLKKKVMKAPVEELDHEGQRLLQCIRSSDGFSGRNCISGSADFQSVVPKIASLLDKLHSTRQHLHQMWHLRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHAVELQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYAAQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKSEQFLSGVETWCKMCSEGGLPSEMQDLESAIHHHQTLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNAESLTATANYSKAVHCILDVVHEVLHHQRRLENIWQHRKVRLHQRLQLCVFQQDVQQVIDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKELLEEVCSDSVDSVQELIKQFQQQQTATLEATLNVIKEGEDLMQQLRDSAMSSNKMPHTSSIAHIEGVLQQLEEAQGHMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLVRQMNEYTGEEPSLAEQRLQRHAERKLAMTNMTYEVMQQGQDLHQYIMEVQASGIELTGEKEVDLATQVQELLEFLHEKQHELDMSAEHTQKRLEQCVQLRHLQAEVKQVLGWIRNGESMLDASMVNASSLSEAEQLQREHEQFQLAIESLFHANSLQKTHQSALQVQQKAEIMLQAGHFDPEAIRSCAEKVAVHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGAHDKLGSSSETDHVLPLISKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSSQGSSSNTRGPEQQVKAILNELLQRENRVLHFWTMKKRRLDQCQQYVVFERNAKQALEWIEEAGEVYLATHTCIGDSMEKTQGLLKEYEEFCISAKQTKEKVKMLIQLADNFVEKGHMHVTDLKKCLTAVDKRYRDFSMRMGKYRCDLENVLGISSEDNKDLELDIIPASLTGPEVKLRDPNHEVNEEKRKSARKKEFIIAELLQTEKAYVRDLHECLETYLWEMTNGVEEIPPGIANKEHIIFGNIQEIYDFHNNIFLKELINYEQLPEDVGHCFVTWADKFNIYVTYCKNKPDSSQLILDHAGSFFDEIQKRHSLTNSISSYLIKPVQRITKYQLLLKELLSCCEEGKGEIKDGLEVMLSVPKRANDAMHVSMLEGFDANLDVQGELILQDTFQVWDPKSLIRKGRDRHLFLFEISLVFSKEIKDSAGRSKYIFKNKLLTSELGVTEHVEGDPCKFALWVGRTPSSDNKTVLKASSIEVKQEWIKNIREVIQERIIHLKGALKEPIHIPKTPAKPRNNSKRDAGEDADSQGDGSSQPDTISIASRTSQNTVDSDKLSGGCELTVVLQDFTASSSSELSIQTGQTVELLERPSDRPGWCLVRTTEKTPPQEGLVPSSALCISHSRSSVEMDCFFPAGKENYTVSGPEGKTESVATLQPQPSLNSLQSSSPGPKRSGNTLKKWLTSPVRRLSHGSNIKKIPSKQKQKRDGRKSIDMGPPELQDDTLEERVQKEEGTLSKSSSGMHSGGEEEPEDESHTPLPPPMEIIKDPSAQEEKSSVLLASRQSSADVPSAAELVSAIEKLVKTKMTLEPGSYPGFSSVNPAEQSPIPPRNPELEQKAKALRGRMFVVKELIQTEKDYVKDLGIVVEGFMKRIEEKGVPDNMKGKDKIVFGNIHQIYDWHKDFFVGELEKCLEDHEKLPELFCKHERRLHMYVVYCQNKPKSEFIVAEYDSYFEGIQQDINSRLSISDFLIKPIQRITKYQLLLKDVLKYSTKAGLDCQDIENAVDLMSQVPKLCNDMMNLGRLQGYEGKLTSQGKLLQQETFFVTEQDSGVLSRSKERRVFLFEQIVIFSELLRKGSSTPGYQFKKSIKVSFLDLEEHVENDPCKFVLSCRGSAERFTLQAANTDIKQVWVQHITQLLDLQSNFLSALQSPIEYQKERSGSQSLTRNSSGRMGQSNSRPCSTVSLGAEKPSLSGRSSTPLKLSTSNGGPCSERYRRQFEGLGCNGTSSSLMVTQDYTALKENEICVTQGEAVQVLATNQQNMYLVYRPANSQSPPAEGWVPGHILGPVTKPLIDTTADASIKKSLSWNTLRARKRAEKDSTPLKSDFKVENGLRKPKEILSSKVKVKESRSSDESECEDELDPKTSMELLNPNFIQEVAPEFLTPLTDVTCALGETVVLCCKVCARPKPTITLKGPDQSLLANNNRFTINIRETGDIVLKICNLMPQDTGIYTCVAVNDHGTASSSASIKVQGIPAAPARPVAQEASSTAVIVHWLPPASSANSAISSYTVEYRQEDSLVWQQLVVSTADVFVKIDNLIPGGHYQFRVSASNPWGTSPPSEPSNMVTLSSTASTYDGAGIQWKDNFESAFTELCEIGRGRFSVVRKCLSKATKQEVAVKFVNKKMHKKEQVAHEADILRHVQHPQLVSLIDTYESPTAYMLILELVEDGRLLDYLVAHDELMEEKVAFFIKDTLEALQHLHTCRVAHLDLKPENLLVDLHVPVPCVKLSDLGDAVQVSSHRYVHLLLGNPEFAAPELIQGTPVSLSTDVWSVGVLAYVMLSGVSPFLDESLEETCVNICRLDFCFPQEYFSDISQAAKDFIVSTLNQDPRKRPSSASCLQHPWVSAHSGDYSKTPLDTVRLAAFIDRRKQLHDVRPVTNIKGMVSSSMGHTL

Target ORF information:

RefSeq Version	XM_021478826.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kalirin RhoGEF kinase a (kalrna), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021478826.1

ORF Insert Sequence:

ATGTATTTAT GGAGATACGG ATGTGGCATG CTGTCTGCCT CTCTCATCAG AAGAAAGACA 
GGGTCTTTCA GAAGTGAAGG AATTAAAGCC TCTGATGTCC TTCCTGTGCT GAGAGAAAAA 
ATAGCATTTG TGTCAGGCGG TCGTGATAAG CGAGGTGGCC CTATACTGAC CTTTCCTGCA 
AGGAGTAACC ATGACCGAAT AAAACAAGAG GACCTGAGAC GGCTCGTAAC ATACCTGTCC 
ACTGTGCCCA GTGAGGACGT TTGTAAGAGA GGCTTCACTG TGATTATTGA TATGCGTGGC 
TCTAAATGGG AGCTAATAAA GCCACTGCTG AAGACGCTAC AGGAAGCATT TCCAGCTGAG 
ATCTGCGTCG CTCTCATCAT CAAACCAGAC AACTTCTGGC AGAAGCAGAA GACTAACTTT 
GGTAGCGCCA AGTTCACCTT TGAGACTAGC ATGGTATCAG TGGAGGGCTT GACCAAACTA 
GTGGACCCAT CCCAGCTAAC GGACGACTTT GAAGGTTCTC TGGAATACAA TCATGAGGAA 
TGGCTTGAGC TCCGGGTGGC TCTGGAGGAG TTCCTGGCCA GCGCCGTTCA CCTCCTCTCT 
CGCCTGGAAG ATCTACAGGA GACTCTGGCT AAGAAGGAGT TCCCGGTGGA TGTGGAAGCC 
TCTCGTAGAC TCATCGATGA GCACACACAG CTGAAGAAGA AGGTGATGAA GGCTCCGGTG 
GAAGAGCTGG ACCACGAAGG CCAGAGACTC TTGCAGTGCA TCCGCTCCAG CGACGGCTTC 
TCAGGGAGGA ACTGCATTTC TGGAAGTGCC GACTTCCAGA GCGTGGTTCC GAAGATCGCC 
AGTCTGCTGG ATAAACTCCA CAGCACTCGA CAGCACTTGC ACCAGATGTG GCACCTACGA 
AAACTCAAGC TGGATCAGTG CTTTCAGCTC CGACTGTTTG AACAGGACGC AGAGAAGATG 
TTTGACTGGA TCAGTCACAA TAAGGAATTG TTTCTTCAGA GCCACACGGA GATCGGTGTG 
AGCTACCAGC ATGCTGTGGA GCTACAGACG CAACACAACC ACTTTGCCAT GAACTCAATG 
AATGCCTATG TGAACATAAA CCGCATCATG TCGGTGGCGT CGCGGCTGTC GGAGGCGGGC 
CACTACGCCG CTCAGCAGAT CAAACAGATC AGCACTCAGC TGGATCAGGA GTGGAAGAGC 
TTCGCGGCTG CTCTCGATGA ACGCAGCACC ATCCTTGCCA TGTCTGCCGT CTTCCATCAG 
AAATCTGAAC AGTTTTTGTC CGGTGTGGAA ACATGGTGTA AGATGTGCAG TGAAGGCGGC 
TTGCCATCAG AGATGCAGGA TCTGGAATCG GCCATTCATC ACCATCAGAC GCTCTATGAA 
CAAGTCACAC AAGCATACAC CGAGGTGAGT CAGGACGGTA AGGCTTTGCT GGACGTTCTT 
CAGCGGCCGT TGAGTCCTGG GAACGCAGAG TCACTGACTG CGACAGCGAA CTACTCTAAA 
GCCGTGCACT GTATACTGGA CGTGGTCCAC GAGGTCCTGC ATCATCAGAG ACGTCTGGAG 
AACATCTGGC AGCACCGTAA AGTCCGTCTG CATCAGAGAC TGCAGCTCTG TGTCTTCCAG 
CAGGACGTCC AACAGGTGAT TGACTGGATA GAGAATCACG GGGAGGCTTT CCTCAGCAAA 
CACACTGGTG TGGGCAAATC CCTGCACAGA GCCAGAGCCC TCCAGAAACG CCATGATGAC 
TTTGAGGAGG TGGCACAGAA TACATACACT AATGCAGATA AGCTGCTGGA AGCTGCTGAG 
CAGTTGGCTC AGACGGGCGA GTGTGACCCT GAGGAGATCT ACAAGGCAGC GCGACACCTG 
GAAGTCAGGA TCCAGGACTT TGTTAGGCGA GTGGAGCAAA GAAAACTACT GCTGGACATG 
TCTGTCTCTT TCCACACGCA CACTAAAGAG TTGTGGACGT GGATGGAGGA CCTGCAGAAA 
GAGCTGCTGG AGGAAGTTTG TTCTGACTCA GTCGATTCAG TTCAGGAGCT GATAAAACAG 
TTTCAGCAGC AGCAGACGGC CACGCTGGAG GCAACACTCA ATGTCATTAA AGAGGGAGAA 
GATCTTATGC AACAACTCCG AGATTCAGCA ATGAGCAGTA ATAAGATGCC CCACACCAGC 
TCCATCGCTC ACATTGAGGG CGTCCTGCAG CAGCTAGAGG AGGCGCAGGG CCACATGGAG 
GAACTCTTCC ATGAGAGAAA GATCAAACTC GACATCTTCC TGCAACTGCG CATCTTTGAG 
CAGTACACCA TTGAGGTAAC GGCAGAGTTA GACGCGTGGA ATGAAGACTT GGTTCGTCAG 
ATGAATGAGT ATACTGGAGA AGAACCCAGT CTGGCTGAGC AGAGACTCCA GCGGCACGCA 
GAGAGGAAAC TGGCCATGAC TAACATGACC TACGAAGTTA TGCAGCAGGG TCAGGACCTG 
CATCAGTACA TCATGGAAGT CCAAGCTTCA GGTATTGAGT TGACCGGAGA GAAGGAGGTG 
GATCTGGCAA CACAAGTTCA GGAGCTGCTG GAGTTCCTTC ATGAGAAACA GCACGAGTTG 
GACATGAGCG CCGAACACAC ACAGAAGCGC CTGGAGCAGT GTGTGCAGCT CCGCCACCTG 
CAGGCTGAGG TTAAACAGGT TCTGGGGTGG ATTCGTAATG GTGAATCTAT GTTGGATGCC 
AGTATGGTGA ATGCAAGTTC ACTGTCAGAG GCCGAACAAC TACAGAGAGA ACATGAACAG 
TTCCAGCTGG CCATAGAGTC TTTGTTCCAC GCTAACTCTC TCCAGAAAAC CCATCAGAGT 
GCACTACAGG TCCAGCAGAA GGCAGAAATC ATGTTACAGG CTGGACACTT TGACCCCGAG 
GCCATCCGCA GCTGTGCAGA GAAGGTGGCC GTGCACTGGC AGCAGCTCAT GCTCAAGATG 
GAGGACAGAC TCAAACTGGT TAATGCCTCG GTGGCCTTCT ACAAGACGTC AGAACAGGTG 
TGTAGTGTCC TGGAGAGTCT GGAGCAGGAG TACCGCAGAG ATGAAGACTG GTGTGGAGCT 
CATGATAAAC TAGGATCTTC ATCAGAGACT GATCACGTTC TGCCTCTCAT CAGCAAACAC 
CTGGAGCAGA AAGAAGCTTT TCTCAAGGCA TGCACTCTGG CCAGGCGCAA TGCTGAGGTT 
TTTCTGAAAT ACATCCACCG GAACAATGTC AGCTCTCAGG GATCATCTAG CAACACTCGT 
GGCCCTGAGC AACAGGTCAA AGCCATCCTG AATGAACTGC TGCAGAGGGA AAACAGAGTT 
TTGCATTTCT GGACCATGAA GAAGCGGCGT TTGGATCAGT GTCAGCAGTA TGTGGTGTTT 
GAACGCAATG CCAAACAGGC ATTAGAGTGG ATTGAGGAGG CAGGAGAGGT CTATCTGGCC 
ACTCACACCT GCATCGGAGA CTCTATGGAG AAAACTCAGG GACTCCTCAA AGAATACGAA 
GAATTCTGCA TCTCCGCAAA GCAAACTAAG GAGAAAGTGA AGATGCTGAT CCAGCTGGCG 
GATAACTTTG TTGAGAAAGG ACACATGCAT GTCACCGACT TGAAGAAGTG TCTGACGGCA 
GTGGACAAGC GCTACAGGGA CTTCTCCATG CGCATGGGCA AATACCGCTG TGACCTGGAA 
AATGTGCTGG GCATCAGCTC TGAGGATAAT AAGGATCTGG AGCTGGATAT CATCCCAGCC 
AGTCTGACTG GCCCAGAGGT TAAACTCCGT GACCCCAACC ACGAGGTGAA TGAAGAAAAG 
AGGAAATCAG CCCGGAAGAA AGAGTTTATT ATAGCAGAGC TGTTGCAGAC AGAAAAGGCT 
TATGTGAGGG ACTTACATGA ATGCTTAGAG ACATATTTGT GGGAGATGAC CAATGGTGTG 
GAGGAAATTC CTCCAGGAAT CGCCAACAAA GAGCACATCA TCTTTGGCAA CATTCAGGAG 
ATTTACGACT TCCACAATAA TATATTCCTG AAGGAGCTGA TCAATTATGA GCAACTGCCT 
GAGGATGTGG GACATTGCTT TGTGACATGG GCCGATAAGT TTAACATTTA TGTGACATAC 
TGCAAAAACA AACCAGATTC CAGTCAGCTC ATCCTAGACC ATGCTGGGAG TTTTTTTGAT 
GAGATTCAGA AGAGACACAG TCTGACCAAC TCTATATCAT CATACCTCAT CAAACCTGTA 
CAGAGGATCA CCAAATATCA GCTGCTGCTC AAGGAGCTGC TGTCCTGCTG TGAAGAAGGC 
AAAGGTGAAA TTAAAGATGG CCTGGAGGTC ATGCTGAGTG TGCCAAAGAG AGCCAATGAC 
GCCATGCACG TCAGCATGCT GGAGGGTTTT GATGCAAATT TGGATGTTCA GGGTGAACTC 
ATCCTCCAAG ACACCTTCCA GGTGTGGGAC CCCAAATCAC TAATCCGGAA GGGGCGAGAC 
CGCCACCTCT TTCTCTTCGA GATCTCCCTG GTCTTCAGTA AAGAGATCAA AGACTCAGCA 
GGGCGATCCA AATACATCTT TAAAAACAAA CTACTGACTT CTGAATTGGG GGTGACAGAG 
CATGTAGAGG GGGACCCGTG CAAATTTGCT CTGTGGGTGG GACGCACACC ATCGTCTGAC 
AACAAGACTG TCCTCAAGGC ATCTAGTATT GAGGTGAAGC AAGAATGGAT TAAAAACATC 
CGAGAGGTCA TTCAGGAGAG AATAATTCAC CTGAAGGGGG CGCTAAAGGA ACCTATCCAC 
ATTCCCAAAA CTCCAGCCAA ACCTCGCAAT AACAGCAAGA GGGACGCTGG TGAGGATGCT 
GACAGCCAGG GAGACGGCAG CAGTCAGCCG GACACCATCT CCATAGCATC CAGAACCTCT 
CAGAACACTG TAGACAGTGA CAAGCTCTCT GGAGGGTGTG AGCTGACGGT GGTCCTGCAG 
GACTTTACAG CAAGCAGCTC ATCAGAGCTC AGCATCCAGA CGGGTCAGAC AGTGGAGCTG 
CTGGAGCGGC CCAGTGACCG TCCAGGATGG TGTCTGGTAC GGACCACCGA AAAGACGCCT 
CCTCAAGAGG GACTGGTGCC CAGCAGTGCT CTGTGTATCT CGCACTCACG CAGCAGTGTG 
GAGATGGACT GCTTTTTCCC AGCAGGCAAA GAAAACTATA CAGTTAGTGG CCCAGAAGGA 
AAGACAGAGT CAGTGGCCAC ACTGCAGCCA CAGCCATCCC TGAACTCTCT GCAGAGTAGC 
TCTCCTGGAC CCAAGCGCTC TGGGAATACA CTGAAGAAAT GGCTGACCAG TCCTGTCCGA 
CGTCTAAGTC ATGGCAGTAA CATCAAGAAG ATCCCAAGTA AACAGAAACA GAAACGTGAT 
GGTCGCAAGA GTATTGATAT GGGGCCGCCC GAACTACAGG ATGACACCTT GGAAGAGCGA 
GTGCAAAAAG AAGAGGGCAC ACTCTCCAAG TCTTCGTCTG GGATGCACAG TGGAGGAGAG 
GAAGAGCCTG AGGACGAGTC TCACACTCCT CTACCTCCAC CAATGGAGAT CATTAAAGAC 
CCGTCGGCTC AGGAAGAGAA GTCGTCTGTG CTTCTGGCTT CCCGGCAGAG CTCGGCTGAT 
GTCCCCAGTG CTGCTGAGCT TGTCAGTGCT ATAGAAAAAC TGGTCAAAAC TAAAATGACA 
CTGGAGCCAG GCTCATACCC GGGATTCAGC TCAGTCAATC CAGCAGAGCA GAGCCCGATT 
CCACCCAGAA ACCCAGAACT AGAGCAGAAA GCCAAAGCGC TACGAGGACG AATGTTTGTA 
GTCAAGGAAT TAATCCAAAC GGAAAAAGAC TATGTTAAAG ATCTGGGAAT TGTGGTTGAG 
GGGTTTATGA AGAGAATCGA GGAGAAAGGA GTTCCTGATA ACATGAAAGG AAAAGATAAG 
ATCGTCTTCG GAAACATTCA CCAAATCTAT GACTGGCACA AAGATTTTTT TGTTGGAGAA 
CTGGAGAAAT GCCTGGAAGA TCACGAAAAA CTGCCTGAAC TGTTCTGTAA ACATGAGAGA 
CGGTTACACA TGTATGTAGT TTACTGTCAG AACAAACCCA AATCCGAGTT CATCGTTGCA 
GAATATGATT CATACTTTGA GGGGATTCAG CAAGACATTA ACTCCAGACT GAGCATCAGC 
GATTTTCTCA TTAAACCCAT TCAGAGAATT ACTAAATACC AGCTTTTATT AAAGGATGTT 
TTGAAGTACA GTACAAAAGC AGGACTGGAT TGTCAGGATA TAGAGAATGC AGTTGACCTG 
ATGTCCCAGG TTCCCAAGCT TTGCAATGAC ATGATGAATC TGGGAAGACT GCAGGGATAT 
GAGGGGAAGT TGACGAGTCA AGGAAAACTC TTGCAGCAGG AGACGTTCTT TGTGACCGAG 
CAGGACTCTG GCGTTCTGTC CCGCAGTAAA GAGCGCAGGG TTTTCCTGTT CGAACAAATT 
GTCATCTTCA GCGAGCTGTT ACGTAAAGGC TCTTCCACTC CAGGGTACCA GTTCAAGAAG 
AGCATAAAGG TGAGCTTCCT TGACTTGGAA GAGCATGTTG AAAATGATCC ATGCAAGTTT 
GTGCTGTCGT GTCGTGGGTC GGCAGAACGT TTTACCCTTC AAGCAGCAAA CACGGACATT 
AAACAAGTCT GGGTTCAGCA TATCACCCAA CTACTAGATT TACAAAGCAA CTTCTTGTCA 
GCTCTGCAGT CTCCTATTGA GTATCAGAAG GAGCGTAGTG GCTCTCAATC TCTGACCCGG 
AACTCTTCTG GGCGGATGGG TCAGTCCAAC AGCAGGCCCT GCTCCACGGT TTCACTCGGA 
GCAGAGAAAC CCTCTCTGTC TGGACGGAGC TCCACCCCGC TAAAGCTATC TACCTCCAAT 
GGTGGTCCAT GCTCTGAAAG ATACCGCAGG CAGTTTGAAG GCTTGGGCTG CAACGGTACA 
TCGTCCTCCT TGATGGTGAC TCAAGATTAT ACTGCACTGA AAGAGAATGA GATCTGTGTC 
ACACAGGGTG AGGCCGTGCA GGTACTGGCC ACTAACCAGC AGAACATGTA CCTTGTGTAC 
CGACCCGCAA ATAGCCAATC ACCACCTGCA GAGGGCTGGG TGCCGGGACA CATCCTAGGC 
CCCGTCACAA AACCTCTCAT AGACACTACT GCAGACGCAA GCATCAAGAA ATCTCTTTCG 
TGGAACACAC TTCGTGCTCG TAAGCGTGCA GAAAAGGACA GCACGCCCCT CAAAAGTGAC 
TTTAAAGTTG AGAATGGACT GCGTAAGCCA AAGGAAATTC TCAGCAGCAA AGTCAAAGTG 
AAAGAGTCCC GCAGTTCTGA TGAGTCTGAA TGTGAGGATG AACTAGACCC CAAAACTAGT 
ATGGAGTTAC TGAACCCTAA TTTCATCCAA GAGGTCGCTC CAGAGTTTCT GACGCCTCTA 
ACAGACGTGA CGTGTGCTCT GGGAGAGACG GTGGTTCTGT GCTGTAAAGT GTGTGCTCGA 
CCCAAACCCA CAATCACCTT GAAAGGACCT GATCAAAGTC TGCTGGCCAA CAATAACCGC 
TTCACTATTA ATATCAGAGA AACCGGTGAT ATAGTGTTGA AAATCTGCAA TCTAATGCCG 
CAGGACACTG GCATCTACAC GTGTGTTGCT GTGAATGACC ATGGTACAGC CTCCTCATCT 
GCATCCATTA AAGTACAAGG TATCCCAGCA GCCCCTGCTC GACCGGTGGC ACAGGAGGCA 
AGCAGCACTG CAGTGATTGT CCACTGGCTT CCTCCAGCCA GCTCAGCAAA TTCTGCCATC 
TCCAGCTACA CTGTGGAATA TAGGCAGGAA GATTCTCTGG TATGGCAGCA GTTGGTGGTC 
TCCACGGCAG ATGTGTTTGT GAAGATTGAC AATCTGATTC CTGGTGGGCA CTACCAGTTC 
AGAGTCAGTG CCAGTAATCC CTGGGGCACC AGCCCGCCTA GTGAACCCTC AAACATGGTG 
ACATTATCCA GCACAGCATC AACATATGAT GGCGCTGGAA TCCAGTGGAA AGACAACTTT 
GAATCTGCTT TTACAGAATT ATGTGAAATT GGGCGGGGTC GTTTCTCTGT GGTGAGGAAG 
TGTCTCAGTA AAGCCACTAA GCAGGAGGTT GCTGTGAAGT TTGTGAATAA GAAGATGCAC 
AAGAAAGAAC AGGTGGCCCA TGAAGCTGAT ATTCTACGCC ATGTCCAGCA CCCCCAACTG 
GTGTCACTGA TTGATACATA CGAGTCGCCT ACAGCCTACA TGCTGATTCT TGAACTTGTT 
GAGGATGGAC GTCTTCTTGA CTATCTGGTA GCTCATGATG AGCTGATGGA GGAGAAAGTG 
GCATTCTTTA TTAAAGATAC ACTGGAAGCT CTGCAGCACT TGCACACCTG CAGAGTGGCT 
CATCTAGACC TCAAGCCTGA AAACTTGTTG GTGGACCTCC ACGTTCCGGT ACCCTGCGTG 
AAGCTCTCAG ATCTAGGTGA TGCTGTACAG GTTTCAAGTC ACCGCTACGT ACATCTCCTC 
CTGGGTAACC CCGAGTTCGC TGCTCCAGAG CTCATCCAGG GCACTCCAGT GTCCCTCTCT 
ACAGATGTGT GGAGTGTAGG GGTGCTGGCC TACGTCATGC TTAGTGGAGT CTCCCCATTC 
CTGGATGAGA GTCTGGAGGA GACCTGCGTC AACATCTGCA GGCTGGACTT CTGCTTTCCT 
CAAGAGTACT TCTCCGATAT AAGCCAGGCA GCCAAAGACT TCATTGTGTC CACGCTTAAC 
CAGGATCCCA GGAAGAGGCC AAGCTCGGCC TCGTGTCTTC AGCACCCATG GGTCAGTGCT 
CATAGTGGAG ATTACTCCAA AACACCTCTG GATACTGTCA GACTCGCTGC ATTTATAGAC 
AGACGCAAAC AGCTACATGA TGTCAGGCCT GTCACAAACA TTAAAGGAAT GGTCAGCAGC 
AGTATGGGAC ACACCTTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kalrna ( XM_017357741.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213230.1 Danio rerio kalirin RhoGEF kinase a (kalrna), transcript variant X1, mRNA.

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CloneID	ODa60287
Clone ID Related Accession (Same CDS sequence)	XM_017357741.2 , XM_017357741.1
Accession Version	XM_017357741.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kalirin isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017357741.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTG CTGAAGCGCC GGAGGACGGA CCCAATGATT CGGACACGGA TGCATTCTTC 
AAAACAGGGT CTTTCAGAAG TGAAGGAATT AAAGCCTCTG ATGTCCTTCC TGTGCTGAGA 
GAAAAAATAG CATTTGTGTC AGGCGGTCGT GATAAGCGAG GTGGCCCTAT ACTGACCTTT 
CCTGCAAGGA GTAACCATGA CCGAATAAAA CAAGAGGACC TGAGACGGCT CGTAACATAC 
CTGTCCACTG TGCCCAGTGA GGACGTTTGT AAGAGAGGCT TCACTGTGAT TATTGATATG 
CGTGGCTCTA AATGGGAGCT AATAAAGCCA CTGCTGAAGA CGCTACAGGA AGCATTTCCA 
GCTGAGATCT GCGTCGCTCT CATCATCAAA CCAGACAACT TCTGGCAGAA GCAGAAGACT 
AACTTTGGTA GCGCCAAGTT CACCTTTGAG ACTAGCATGG TATCAGTGGA GGGCTTGACC 
AAACTAGTGG ACCCATCCCA GCTAACGGAC GACTTTGAAG GTTCTCTGGA ATACAATCAT 
GAGGAATGGC TTGAGCTCCG GGTGGCTCTG GAGGAGTTCC TGGCCAGCGC CGTTCACCTC 
CTCTCTCGCC TGGAAGATCT ACAGGAGACT CTGGCTAAGA AGGAGTTCCC GGTGGATGTG 
GAAGCCTCTC GTAGACTCAT CGATGAGCAC ACACAGCTGA AGAAGAAGGT GATGAAGGCT 
CCGGTGGAAG AGCTGGACCA CGAAGGCCAG AGACTCTTGC AGTGCATCCG CTCCAGCGAC 
GGCTTCTCAG GGAGGAACTG CATTTCTGGA AGTGCCGACT TCCAGAGCGT GGTTCCGAAG 
ATCGCCAGTC TGCTGGATAA ACTCCACAGC ACTCGACAGC ACTTGCACCA GATGTGGCAC 
CTACGAAAAC TCAAGCTGGA TCAGTGCTTT CAGCTCCGAC TGTTTGAACA GGACGCAGAG 
AAGATGTTTG ACTGGATCAG TCACAATAAG GAATTGTTTC TTCAGAGCCA CACGGAGATC 
GGTGTGAGCT ACCAGCATGC TGTGGAGCTA CAGACGCAAC ACAACCACTT TGCCATGAAC 
TCAATGAATG CCTATGTGAA CATAAACCGC ATCATGTCGG TGGCGTCGCG GCTGTCGGAG 
GCGGGCCACT ACGCCGCTCA GCAGATCAAA CAGATCAGCA CTCAGCTGGA TCAGGAGTGG 
AAGAGCTTCG CGGCTGCTCT CGATGAACGC AGCACCATCC TTGCCATGTC TGCCGTCTTC 
CATCAGAAAT CTGAACAGTT TTTGTCCGGT GTGGAAACAT GGTGTAAGAT GTGCAGTGAA 
GGCGGCTTGC CATCAGAGAT GCAGGATCTG GAATCGGCCA TTCATCACCA TCAGACGCTC 
TATGAACAAG TCACACAAGC ATACACCGAG GTGAGTCAGG ACGGTAAGGC TTTGCTGGAC 
GTTCTTCAGC GGCCGTTGAG TCCTGGGAAC GCAGAGTCAC TGACTGCGAC AGCGAACTAC 
TCTAAAGCCG TGCACTGTAT ACTGGACGTG GTCCACGAGG TCCTGCATCA TCAGAGACGT 
CTGGAGAACA TCTGGCAGCA CCGTAAAGTC CGTCTGCATC AGAGACTGCA GCTCTGTGTC 
TTCCAGCAGG ACGTCCAACA GGTGATTGAC TGGATAGAGA ATCACGGGGA GGCTTTCCTC 
AGCAAACACA CTGGTGTGGG CAAATCCCTG CACAGAGCCA GAGCCCTCCA GAAACGCCAT 
GATGACTTTG AGGAGGTGGC ACAGAATACA TACACTAATG CAGATAAGCT GCTGGAAGCT 
GCTGAGCAGT TGGCTCAGAC GGGCGAGTGT GACCCTGAGG AGATCTACAA GGCAGCGCGA 
CACCTGGAAG TCAGGATCCA GGACTTTGTT AGGCGAGTGG AGCAAAGAAA ACTACTGCTG 
GACATGTCTG TCTCTTTCCA CACGCACACT AAAGAGTTGT GGACGTGGAT GGAGGACCTG 
CAGAAAGAGC TGCTGGAGGA AGTTTGTTCT GACTCAGTCG ATTCAGTTCA GGAGCTGATA 
AAACAGTTTC AGCAGCAGCA GACGGCCACG CTGGAGGCAA CACTCAATGT CATTAAAGAG 
GGAGAAGATC TTATGCAACA ACTCCGAGAT TCAGCAATGA GCAGTAATAA GATGCCCCAC 
ACCAGCTCCA TCGCTCACAT TGAGGGCGTC CTGCAGCAGC TAGAGGAGGC GCAGGGCCAC 
ATGGAGGAAC TCTTCCATGA GAGAAAGATC AAACTCGACA TCTTCCTGCA ACTGCGCATC 
TTTGAGCAGT ACACCATTGA GGTAACGGCA GAGTTAGACG CGTGGAATGA AGACTTGGTT 
CGTCAGATGA ATGAGTATAC TGGAGAAGAA CCCAGTCTGG CTGAGCAGAG ACTCCAGCGG 
CACGCAGAGA GGAAACTGGC CATGACTAAC ATGACCTACG AAGTTATGCA GCAGGGTCAG 
GACCTGCATC AGTACATCAT GGAAGTCCAA GCTTCAGGTA TTGAGTTGAC CGGAGAGAAG 
GAGGTGGATC TGGCAACACA AGTTCAGGAG CTGCTGGAGT TCCTTCATGA GAAACAGCAC 
GAGTTGGACA TGAGCGCCGA ACACACACAG AAGCGCCTGG AGCAGTGTGT GCAGCTCCGC 
CACCTGCAGG CTGAGGTTAA ACAGGTTCTG GGGTGGATTC GTAATGGTGA ATCTATGTTG 
GATGCCAGTA TGGTGAATGC AAGTTCACTG TCAGAGGCCG AACAACTACA GAGAGAACAT 
GAACAGTTCC AGCTGGCCAT AGAGTCTTTG TTCCACGCTA ACTCTCTCCA GAAAACCCAT 
CAGAGTGCAC TACAGGTCCA GCAGAAGGCA GAAATCATGT TACAGGCTGG ACACTTTGAC 
CCCGAGGCCA TCCGCAGCTG TGCAGAGAAG GTGGCCGTGC ACTGGCAGCA GCTCATGCTC 
AAGATGGAGG ACAGACTCAA ACTGGTTAAT GCCTCGGTGG CCTTCTACAA GACGTCAGAA 
CAGGTGTGTA GTGTCCTGGA GAGTCTGGAG CAGGAGTACC GCAGAGATGA AGACTGGTGT 
GGAGCTCATG ATAAACTAGG ATCTTCATCA GAGACTGATC ACGTTCTGCC TCTCATCAGC 
AAACACCTGG AGCAGAAAGA AGCTTTTCTC AAGGCATGCA CTCTGGCCAG GCGCAATGCT 
GAGGTTTTTC TGAAATACAT CCACCGGAAC AATGTCAGCT CTCAGGGATC ATCTAGCAAC 
ACTCGTGGCC CTGAGCAACA GGTCAAAGCC ATCCTGAATG AACTGCTGCA GAGGGAAAAC 
AGAGTTTTGC ATTTCTGGAC CATGAAGAAG CGGCGTTTGG ATCAGTGTCA GCAGTATGTG 
GTGTTTGAAC GCAATGCCAA ACAGGCATTA GAGTGGATTG AGGAGGCAGG AGAGGTCTAT 
CTGGCCACTC ACACCTGCAT CGGAGACTCT ATGGAGAAAA CTCAGGGACT CCTCAAAGAA 
TACGAAGAAT TCTGCATCTC CGCAAAGCAA ACTAAGGAGA AAGTGAAGAT GCTGATCCAG 
CTGGCGGATA ACTTTGTTGA GAAAGGACAC ATGCATGTCA CCGACTTGAA GAAGTGTCTG 
ACGGCAGTGG ACAAGCGCTA CAGGGACTTC TCCATGCGCA TGGGCAAATA CCGCTGTGAC 
CTGGAAAATG TGCTGGGCAT CAGCTCTGAG GATAATAAGG ATCTGGAGCT GGATATCATC 
CCAGCCAGTC TGACTGGCCC AGAGGTTAAA CTCCGTGACC CCAACCACGA GGTGAATGAA 
GAAAAGAGGA AATCAGCCCG GAAGAAAGAG TTTATTATAG CAGAGCTGTT GCAGACAGAA 
AAGGCTTATG TGAGGGACTT ACATGAATGC TTAGAGACAT ATTTGTGGGA GATGACCAAT 
GGTGTGGAGG AAATTCCTCC AGGAATCGCC AACAAAGAGC ACATCATCTT TGGCAACATT 
CAGGAGATTT ACGACTTCCA CAATAATATA TTCCTGAAGG AGCTGATCAA TTATGAGCAA 
CTGCCTGAGG ATGTGGGACA TTGCTTTGTG ACATGGGCCG ATAAGTTTAA CATTTATGTG 
ACATACTGCA AAAACAAACC AGATTCCAGT CAGCTCATCC TAGACCATGC TGGGAGTTTT 
TTTGATGAGA TTCAGAAGAG ACACAGTCTG ACCAACTCTA TATCATCATA CCTCATCAAA 
CCTGTACAGA GGATCACCAA ATATCAGCTG CTGCTCAAGG AGCTGCTGTC CTGCTGTGAA 
GAAGGCAAAG GTGAAATTAA AGATGGCCTG GAGGTCATGC TGAGTGTGCC AAAGAGAGCC 
AATGACGCCA TGCACGTCAG CATGCTGGAG GGTTTTGATG CAAATTTGGA TGTTCAGGGT 
GAACTCATCC TCCAAGACAC CTTCCAGGTG TGGGACCCCA AATCACTAAT CCGGAAGGGG 
CGAGACCGCC ACCTCTTTCT CTTCGAGATC TCCCTGGTCT TCAGTAAAGA GATCAAAGAC 
TCAGCAGGGC GATCCAAATA CATCTTTAAA AACAAACTAC TGACTTCTGA ATTGGGGGTG 
ACAGAGCATG TAGAGGGGGA CCCGTGCAAA TTTGCTCTGT GGGTGGGACG CACACCATCG 
TCTGACAACA AGACTGTCCT CAAGGCATCT AGTATTGAGG TGAAGCAAGA ATGGATTAAA 
AACATCCGAG AGGTCATTCA GGAGAGAATA ATTCACCTGA AGGGGGCGCT AAAGGAACCT 
ATCCACATTC CCAAAACTCC AGCCAAACCT CGCAATAACA GCAAGAGGGA CGCTGGTGAG 
GATGCTGACA GCCAGGGAGA CGGCAGCAGT CAGCCGGACA CCATCTCCAT AGCATCCAGA 
ACCTCTCAGA ACACTGTAGA CAGTGACAAG CTCTCTGGAG GGTGTGAGCT GACGGTGGTC 
CTGCAGGACT TTACAGCAAG CAGCTCATCA GAGCTCAGCA TCCAGACGGG TCAGACAGTG 
GAGCTGCTGG AGCGGCCCAG TGACCGTCCA GGATGGTGTC TGGTACGGAC CACCGAAAAG 
ACGCCTCCTC AAGAGGGACT GGTGCCCAGC AGTGCTCTGT GTATCTCGCA CTCACGCAGC 
AGTGTGGAGA TGGACTGCTT TTTCCCAGCA GGCAAAGAAA ACTATACAGT TAGTGGCCCA 
GAAGGAAAGA CAGAGTCAGT GGCCACACTG CAGCCACAGC CATCCCTGAA CTCTCTGCAG 
AGTAGCTCTC CTGGACCCAA GCGCTCTGGG AATACACTGA AGAAATGGCT GACCAGTCCT 
GTCCGACGTC TAAGTCATGG CAGTAACATC AAGAAGATCC CAAGTAAACA GAAACAGAAA 
CGTGATGGTC GCAAGAGTAT TGATATGGGG CCGCCCGAAC TACAGGATGA CACCTTGGAA 
GAGCGAGTGC AAAAAGAAGA GGGCACACTC TCCAAGTCTT CGTCTGGGAT GCACAGTGGA 
GGAGAGGAAG AGCCTGAGGA CGAGTCTCAC ACTCCTCTAC CTCCACCAAT GGAGATCATT 
AAAGACCCGT CGGCTCAGGA AGAGAAGTCG TCTGTGCTTC TGGCTTCCCG GCAGAGCTCG 
GCTGATGTCC CCAGTGCTGC TGAGCTTGTC AGTGCTATAG AAAAACTGGT CAAAACTAAA 
ATGACACTGG AGCCAGGCTC ATACCCGGGA TTCAGCTCAG TCAATCCAGC AGAGCAGAGC 
CCGATTCCAC CCAGAAACCC AGAACTAGAG CAGAAAGCCA AAGCGCTACG AGGACGAATG 
TTTGTAGTCA AGGAATTAAT CCAAACGGAA AAAGACTATG TTAAAGATCT GGGAATTGTG 
GTTGAGGGGT TTATGAAGAG AATCGAGGAG AAAGGAGTTC CTGATAACAT GAAAGGAAAA 
GATAAGATCG TCTTCGGAAA CATTCACCAA ATCTATGACT GGCACAAAGA TTTTTTTGTT 
GGAGAACTGG AGAAATGCCT GGAAGATCAC GAAAAACTGC CTGAACTGTT CTGTAAACAT 
GAGAGACGGT TACACATGTA TGTAGTTTAC TGTCAGAACA AACCCAAATC CGAGTTCATC 
GTTGCAGAAT ATGATTCATA CTTTGAGGGG ATTCAGCAAG ACATTAACTC CAGACTGAGC 
ATCAGCGATT TTCTCATTAA ACCCATTCAG AGAATTACTA AATACCAGCT TTTATTAAAG 
GATGTTTTGA AGTACAGTAC AAAAGCAGGA CTGGATTGTC AGGATATAGA GAATGCAGTT 
GACCTGATGT CCCAGGTTCC CAAGCTTTGC AATGACATGA TGAATCTGGG AAGACTGCAG 
GGATATGAGG GGAAGTTGAC GAGTCAAGGA AAACTCTTGC AGCAGGAGAC GTTCTTTGTG 
ACCGAGCAGG ACTCTGGCGT TCTGTCCCGC AGTAAAGAGC GCAGGGTTTT CCTGTTCGAA 
CAAATTGTCA TCTTCAGCGA GCTGTTACGT AAAGGCTCTT CCACTCCAGG GTACCAGTTC 
AAGAAGAGCA TAAAGGTGAG CTTCCTTGAC TTGGAAGAGC ATGTTGAAAA TGATCCATGC 
AAGTTTGTGC TGTCGTGTCG TGGGTCGGCA GAACGTTTTA CCCTTCAAGC AGCAAACACG 
GACATTAAAC AAGTCTGGGT TCAGCATATC ACCCAACTAC TAGATTTACA AAGCAACTTC 
TTGTCAGCTC TGCAGTCTCC TATTGAGTAT CAGAAGGAGC GTAGTGGCTC TCAATCTCTG 
ACCCGGAACT CTTCTGGGCG GATGGGTCAG TCCAACAGCA GGCCCTGCTC CACGGTTTCA 
CTCGGAGCAG AGAAACCCTC TCTGTCTGGA CGGAGCTCCA CCCCGCTAAA GCTATCTACC 
TCCAATGGTG GTCCATGCTC TGAAAGATAC CGCAGGCAGT TTGAAGGCTT GGGCTGCAAC 
GGTACATCGT CCTCCTTGAT GGTGACTCAA GATTATACTG CACTGAAAGA GAATGAGATC 
TGTGTCACAC AGGGTGAGGC CGTGCAGGTA CTGGCCACTA ACCAGCAGAA CATGTACCTT 
GTGTACCGAC CCGCAAATAG CCAATCACCA CCTGCAGAGG GCTGGGTGCC GGGACACATC 
CTAGGCCCCG TCACAAAACC TCTCATAGAC ACTACTGCAG ACGCAAGCAT CAAGAAATCT 
CTTTCGTGGA ACACACTTCG TGCTCGTAAG CGTGCAGAAA AGGACAGCAC GCCCCTCAAA 
AGTGACTTTA AAGTTGAGAA TGGACTGCGT AAGCCAAAGG AAATTCTCAG CAGCAAAGTC 
AAAGTGAAAG AGTCCCGCAG TTCTGATGAG TCTGAATGTG AGGATGAACT AGACCCCAAA 
ACTAGTATGG AGTTACTGAA CCCTAATTTC ATCCAAGAGG TCGCTCCAGA GTTTCTGACG 
CCTCTAACAG ACGTGACGTG TGCTCTGGGA GAGACGGTGG TTCTGTGCTG TAAAGTGTGT 
GCTCGACCCA AACCCACAAT CACCTTGAAA GGACCTGATC AAAGTCTGCT GGCCAACAAT 
AACCGCTTCA CTATTAATAT CAGAGAAACC GGTGATATAG TGTTGAAAAT CTGCAATCTA 
ATGCCGCAGG ACACTGGCAT CTACACGTGT GTTGCTGTGA ATGACCATGG TACAGCCTCC 
TCATCTGCAT CCATTAAAGT ACAAGGTATC CCAGCAGCCC CTGCTCGACC GGTGGCACAG 
GAGGCAAGCA GCACTGCAGT GATTGTCCAC TGGCTTCCTC CAGCCAGCTC AGCAAATTCT 
GCCATCTCCA GCTACACTGT GGAATATAGG CAGGAAGATT CTCTGGTATG GCAGCAGTTG 
GTGGTCTCCA CGGCAGATGT GTTTGTGAAG ATTGACAATC TGATTCCTGG TGGGCACTAC 
CAGTTCAGAG TCAGTGCCAG TAATCCCTGG GGCACCAGCC CGCCTAGTGA ACCCTCAAAC 
ATGGTGACAT TATCCAGCAC AGCATCAACA TATGATGGCG CTGGAATCCA GTGGAAAGAC 
AACTTTGAAT CTGCTTTTAC AGAATTATGT GAAATTGGGC GGGGTCGTTT CTCTGTGGTG 
AGGAAGTGTC TCAGTAAAGC CACTAAGCAG GAGGTTGCTG TGAAGTTTGT GAATAAGAAG 
ATGCACAAGA AAGAACAGGT GGCCCATGAA GCTGATATTC TACGCCATGT CCAGCACCCC 
CAACTGGTGT CACTGATTGA TACATACGAG TCGCCTACAG CCTACATGCT GATTCTTGAA 
CTTGTTGAGG ATGGACGTCT TCTTGACTAT CTGGTAGCTC ATGATGAGCT GATGGAGGAG 
AAAGTGGCAT TCTTTATTAA AGATACACTG GAAGCTCTGC AGCACTTGCA CACCTGCAGA 
GTGGCTCATC TAGACCTCAA GCCTGAAAAC TTGTTGGTGG ACCTCCACGT TCCGGTACCC 
TGCGTGAAGC TCTCAGATCT AGGTGATGCT GTACAGGTTT CAAGTCACCG CTACGTACAT 
CTCCTCCTGG GTAACCCCGA GTTCGCTGCT CCAGAGCTCA TCCAGGGCAC TCCAGTGTCC 
CTCTCTACAG ATGTGTGGAG TGTAGGGGTG CTGGCCTACG TCATGCTTAG TGGAGTCTCC 
CCATTCCTGG ATGAGAGTCT GGAGGAGACC TGCGTCAACA TCTGCAGGCT GGACTTCTGC 
TTTCCTCAAG AGTACTTCTC CGATATAAGC CAGGCAGCCA AAGACTTCAT TGTGTCCACG 
CTTAACCAGG ATCCCAGGAA GAGGCCAAGC TCGGCCTCGT GTCTTCAGCA CCCATGGGTC 
AGTGCTCATA GTGGAGATTA CTCCAAAACA CCTCTGGATA CTGTCAGACT CGCTGCATTT 
ATAGACAGAC GCAAACAGCT ACATGATGTC AGGCCTGTCA CAAACATTAA AGGAATGGTC 
AGCAGCAGTA TGGGACACAC CTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213230.1
CDS	552..9458
Translation	MNSAEAPEDGPNDSDTDAFFKTGSFRSEGIKASDVLPVLREKIAFVSGGRDKRGGPILTFPARSNHDRIKQEDLRRLVTYLSTVPSEDVCKRGFTVIIDMRGSKWELIKPLLKTLQEAFPAEICVALIIKPDNFWQKQKTNFGSAKFTFETSMVSVEGLTKLVDPSQLTDDFEGSLEYNHEEWLELRVALEEFLASAVHLLSRLEDLQETLAKKEFPVDVEASRRLIDEHTQLKKKVMKAPVEELDHEGQRLLQCIRSSDGFSGRNCISGSADFQSVVPKIASLLDKLHSTRQHLHQMWHLRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHAVELQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYAAQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKSEQFLSGVETWCKMCSEGGLPSEMQDLESAIHHHQTLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNAESLTATANYSKAVHCILDVVHEVLHHQRRLENIWQHRKVRLHQRLQLCVFQQDVQQVIDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKELLEEVCSDSVDSVQELIKQFQQQQTATLEATLNVIKEGEDLMQQLRDSAMSSNKMPHTSSIAHIEGVLQQLEEAQGHMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLVRQMNEYTGEEPSLAEQRLQRHAERKLAMTNMTYEVMQQGQDLHQYIMEVQASGIELTGEKEVDLATQVQELLEFLHEKQHELDMSAEHTQKRLEQCVQLRHLQAEVKQVLGWIRNGESMLDASMVNASSLSEAEQLQREHEQFQLAIESLFHANSLQKTHQSALQVQQKAEIMLQAGHFDPEAIRSCAEKVAVHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGAHDKLGSSSETDHVLPLISKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSSQGSSSNTRGPEQQVKAILNELLQRENRVLHFWTMKKRRLDQCQQYVVFERNAKQALEWIEEAGEVYLATHTCIGDSMEKTQGLLKEYEEFCISAKQTKEKVKMLIQLADNFVEKGHMHVTDLKKCLTAVDKRYRDFSMRMGKYRCDLENVLGISSEDNKDLELDIIPASLTGPEVKLRDPNHEVNEEKRKSARKKEFIIAELLQTEKAYVRDLHECLETYLWEMTNGVEEIPPGIANKEHIIFGNIQEIYDFHNNIFLKELINYEQLPEDVGHCFVTWADKFNIYVTYCKNKPDSSQLILDHAGSFFDEIQKRHSLTNSISSYLIKPVQRITKYQLLLKELLSCCEEGKGEIKDGLEVMLSVPKRANDAMHVSMLEGFDANLDVQGELILQDTFQVWDPKSLIRKGRDRHLFLFEISLVFSKEIKDSAGRSKYIFKNKLLTSELGVTEHVEGDPCKFALWVGRTPSSDNKTVLKASSIEVKQEWIKNIREVIQERIIHLKGALKEPIHIPKTPAKPRNNSKRDAGEDADSQGDGSSQPDTISIASRTSQNTVDSDKLSGGCELTVVLQDFTASSSSELSIQTGQTVELLERPSDRPGWCLVRTTEKTPPQEGLVPSSALCISHSRSSVEMDCFFPAGKENYTVSGPEGKTESVATLQPQPSLNSLQSSSPGPKRSGNTLKKWLTSPVRRLSHGSNIKKIPSKQKQKRDGRKSIDMGPPELQDDTLEERVQKEEGTLSKSSSGMHSGGEEEPEDESHTPLPPPMEIIKDPSAQEEKSSVLLASRQSSADVPSAAELVSAIEKLVKTKMTLEPGSYPGFSSVNPAEQSPIPPRNPELEQKAKALRGRMFVVKELIQTEKDYVKDLGIVVEGFMKRIEEKGVPDNMKGKDKIVFGNIHQIYDWHKDFFVGELEKCLEDHEKLPELFCKHERRLHMYVVYCQNKPKSEFIVAEYDSYFEGIQQDINSRLSISDFLIKPIQRITKYQLLLKDVLKYSTKAGLDCQDIENAVDLMSQVPKLCNDMMNLGRLQGYEGKLTSQGKLLQQETFFVTEQDSGVLSRSKERRVFLFEQIVIFSELLRKGSSTPGYQFKKSIKVSFLDLEEHVENDPCKFVLSCRGSAERFTLQAANTDIKQVWVQHITQLLDLQSNFLSALQSPIEYQKERSGSQSLTRNSSGRMGQSNSRPCSTVSLGAEKPSLSGRSSTPLKLSTSNGGPCSERYRRQFEGLGCNGTSSSLMVTQDYTALKENEICVTQGEAVQVLATNQQNMYLVYRPANSQSPPAEGWVPGHILGPVTKPLIDTTADASIKKSLSWNTLRARKRAEKDSTPLKSDFKVENGLRKPKEILSSKVKVKESRSSDESECEDELDPKTSMELLNPNFIQEVAPEFLTPLTDVTCALGETVVLCCKVCARPKPTITLKGPDQSLLANNNRFTINIRETGDIVLKICNLMPQDTGIYTCVAVNDHGTASSSASIKVQGIPAAPARPVAQEASSTAVIVHWLPPASSANSAISSYTVEYRQEDSLVWQQLVVSTADVFVKIDNLIPGGHYQFRVSASNPWGTSPPSEPSNMVTLSSTASTYDGAGIQWKDNFESAFTELCEIGRGRFSVVRKCLSKATKQEVAVKFVNKKMHKKEQVAHEADILRHVQHPQLVSLIDTYESPTAYMLILELVEDGRLLDYLVAHDELMEEKVAFFIKDTLEALQHLHTCRVAHLDLKPENLLVDLHVPVPCVKLSDLGDAVQVSSHRYVHLLLGNPEFAAPELIQGTPVSLSTDVWSVGVLAYVMLSGVSPFLDESLEETCVNICRLDFCFPQEYFSDISQAAKDFIVSTLNQDPRKRPSSASCLQHPWVSAHSGDYSKTPLDTVRLAAFIDRRKQLHDVRPVTNIKGMVSSSMGHTL

Target ORF information:

RefSeq Version	XM_017357741.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kalirin RhoGEF kinase a (kalrna), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357741.2

ORF Insert Sequence:

ATGAATTCTG CTGAAGCGCC GGAGGACGGA CCCAATGATT CGGACACGGA TGCATTCTTC 
AAAACAGGGT CTTTCAGAAG TGAAGGAATT AAAGCCTCTG ATGTCCTTCC TGTGCTGAGA 
GAAAAAATAG CATTTGTGTC AGGCGGTCGT GATAAGCGAG GTGGCCCTAT ACTGACCTTT 
CCTGCAAGGA GTAACCATGA CCGAATAAAA CAAGAGGACC TGAGACGGCT CGTAACATAC 
CTGTCCACTG TGCCCAGTGA GGACGTTTGT AAGAGAGGCT TCACTGTGAT TATTGATATG 
CGTGGCTCTA AATGGGAGCT AATAAAGCCA CTGCTGAAGA CGCTACAGGA AGCATTTCCA 
GCTGAGATCT GCGTCGCTCT CATCATCAAA CCAGACAACT TCTGGCAGAA GCAGAAGACT 
AACTTTGGTA GCGCCAAGTT CACCTTTGAG ACTAGCATGG TATCAGTGGA GGGCTTGACC 
AAACTAGTGG ACCCATCCCA GCTAACGGAC GACTTTGAAG GTTCTCTGGA ATACAATCAT 
GAGGAATGGC TTGAGCTCCG GGTGGCTCTG GAGGAGTTCC TGGCCAGCGC CGTTCACCTC 
CTCTCTCGCC TGGAAGATCT ACAGGAGACT CTGGCTAAGA AGGAGTTCCC GGTGGATGTG 
GAAGCCTCTC GTAGACTCAT CGATGAGCAC ACACAGCTGA AGAAGAAGGT GATGAAGGCT 
CCGGTGGAAG AGCTGGACCA CGAAGGCCAG AGACTCTTGC AGTGCATCCG CTCCAGCGAC 
GGCTTCTCAG GGAGGAACTG CATTTCTGGA AGTGCCGACT TCCAGAGCGT GGTTCCGAAG 
ATCGCCAGTC TGCTGGATAA ACTCCACAGC ACTCGACAGC ACTTGCACCA GATGTGGCAC 
CTACGAAAAC TCAAGCTGGA TCAGTGCTTT CAGCTCCGAC TGTTTGAACA GGACGCAGAG 
AAGATGTTTG ACTGGATCAG TCACAATAAG GAATTGTTTC TTCAGAGCCA CACGGAGATC 
GGTGTGAGCT ACCAGCATGC TGTGGAGCTA CAGACGCAAC ACAACCACTT TGCCATGAAC 
TCAATGAATG CCTATGTGAA CATAAACCGC ATCATGTCGG TGGCGTCGCG GCTGTCGGAG 
GCGGGCCACT ACGCCGCTCA GCAGATCAAA CAGATCAGCA CTCAGCTGGA TCAGGAGTGG 
AAGAGCTTCG CGGCTGCTCT CGATGAACGC AGCACCATCC TTGCCATGTC TGCCGTCTTC 
CATCAGAAAT CTGAACAGTT TTTGTCCGGT GTGGAAACAT GGTGTAAGAT GTGCAGTGAA 
GGCGGCTTGC CATCAGAGAT GCAGGATCTG GAATCGGCCA TTCATCACCA TCAGACGCTC 
TATGAACAAG TCACACAAGC ATACACCGAG GTGAGTCAGG ACGGTAAGGC TTTGCTGGAC 
GTTCTTCAGC GGCCGTTGAG TCCTGGGAAC GCAGAGTCAC TGACTGCGAC AGCGAACTAC 
TCTAAAGCCG TGCACTGTAT ACTGGACGTG GTCCACGAGG TCCTGCATCA TCAGAGACGT 
CTGGAGAACA TCTGGCAGCA CCGTAAAGTC CGTCTGCATC AGAGACTGCA GCTCTGTGTC 
TTCCAGCAGG ACGTCCAACA GGTGATTGAC TGGATAGAGA ATCACGGGGA GGCTTTCCTC 
AGCAAACACA CTGGTGTGGG CAAATCCCTG CACAGAGCCA GAGCCCTCCA GAAACGCCAT 
GATGACTTTG AGGAGGTGGC ACAGAATACA TACACTAATG CAGATAAGCT GCTGGAAGCT 
GCTGAGCAGT TGGCTCAGAC GGGCGAGTGT GACCCTGAGG AGATCTACAA GGCAGCGCGA 
CACCTGGAAG TCAGGATCCA GGACTTTGTT AGGCGAGTGG AGCAAAGAAA ACTACTGCTG 
GACATGTCTG TCTCTTTCCA CACGCACACT AAAGAGTTGT GGACGTGGAT GGAGGACCTG 
CAGAAAGAGC TGCTGGAGGA AGTTTGTTCT GACTCAGTCG ATTCAGTTCA GGAGCTGATA 
AAACAGTTTC AGCAGCAGCA GACGGCCACG CTGGAGGCAA CACTCAATGT CATTAAAGAG 
GGAGAAGATC TTATGCAACA ACTCCGAGAT TCAGCAATGA GCAGTAATAA GATGCCCCAC 
ACCAGCTCCA TCGCTCACAT TGAGGGCGTC CTGCAGCAGC TAGAGGAGGC GCAGGGCCAC 
ATGGAGGAAC TCTTCCATGA GAGAAAGATC AAACTCGACA TCTTCCTGCA ACTGCGCATC 
TTTGAGCAGT ACACCATTGA GGTAACGGCA GAGTTAGACG CGTGGAATGA AGACTTGGTT 
CGTCAGATGA ATGAGTATAC TGGAGAAGAA CCCAGTCTGG CTGAGCAGAG ACTCCAGCGG 
CACGCAGAGA GGAAACTGGC CATGACTAAC ATGACCTACG AAGTTATGCA GCAGGGTCAG 
GACCTGCATC AGTACATCAT GGAAGTCCAA GCTTCAGGTA TTGAGTTGAC CGGAGAGAAG 
GAGGTGGATC TGGCAACACA AGTTCAGGAG CTGCTGGAGT TCCTTCATGA GAAACAGCAC 
GAGTTGGACA TGAGCGCCGA ACACACACAG AAGCGCCTGG AGCAGTGTGT GCAGCTCCGC 
CACCTGCAGG CTGAGGTTAA ACAGGTTCTG GGGTGGATTC GTAATGGTGA ATCTATGTTG 
GATGCCAGTA TGGTGAATGC AAGTTCACTG TCAGAGGCCG AACAACTACA GAGAGAACAT 
GAACAGTTCC AGCTGGCCAT AGAGTCTTTG TTCCACGCTA ACTCTCTCCA GAAAACCCAT 
CAGAGTGCAC TACAGGTCCA GCAGAAGGCA GAAATCATGT TACAGGCTGG ACACTTTGAC 
CCCGAGGCCA TCCGCAGCTG TGCAGAGAAG GTGGCCGTGC ACTGGCAGCA GCTCATGCTC 
AAGATGGAGG ACAGACTCAA ACTGGTTAAT GCCTCGGTGG CCTTCTACAA GACGTCAGAA 
CAGGTGTGTA GTGTCCTGGA GAGTCTGGAG CAGGAGTACC GCAGAGATGA AGACTGGTGT 
GGAGCTCATG ATAAACTAGG ATCTTCATCA GAGACTGATC ACGTTCTGCC TCTCATCAGC 
AAACACCTGG AGCAGAAAGA AGCTTTTCTC AAGGCATGCA CTCTGGCCAG GCGCAATGCT 
GAGGTTTTTC TGAAATACAT CCACCGGAAC AATGTCAGCT CTCAGGGATC ATCTAGCAAC 
ACTCGTGGCC CTGAGCAACA GGTCAAAGCC ATCCTGAATG AACTGCTGCA GAGGGAAAAC 
AGAGTTTTGC ATTTCTGGAC CATGAAGAAG CGGCGTTTGG ATCAGTGTCA GCAGTATGTG 
GTGTTTGAAC GCAATGCCAA ACAGGCATTA GAGTGGATTG AGGAGGCAGG AGAGGTCTAT 
CTGGCCACTC ACACCTGCAT CGGAGACTCT ATGGAGAAAA CTCAGGGACT CCTCAAAGAA 
TACGAAGAAT TCTGCATCTC CGCAAAGCAA ACTAAGGAGA AAGTGAAGAT GCTGATCCAG 
CTGGCGGATA ACTTTGTTGA GAAAGGACAC ATGCATGTCA CCGACTTGAA GAAGTGTCTG 
ACGGCAGTGG ACAAGCGCTA CAGGGACTTC TCCATGCGCA TGGGCAAATA CCGCTGTGAC 
CTGGAAAATG TGCTGGGCAT CAGCTCTGAG GATAATAAGG ATCTGGAGCT GGATATCATC 
CCAGCCAGTC TGACTGGCCC AGAGGTTAAA CTCCGTGACC CCAACCACGA GGTGAATGAA 
GAAAAGAGGA AATCAGCCCG GAAGAAAGAG TTTATTATAG CAGAGCTGTT GCAGACAGAA 
AAGGCTTATG TGAGGGACTT ACATGAATGC TTAGAGACAT ATTTGTGGGA GATGACCAAT 
GGTGTGGAGG AAATTCCTCC AGGAATCGCC AACAAAGAGC ACATCATCTT TGGCAACATT 
CAGGAGATTT ACGACTTCCA CAATAATATA TTCCTGAAGG AGCTGATCAA TTATGAGCAA 
CTGCCTGAGG ATGTGGGACA TTGCTTTGTG ACATGGGCCG ATAAGTTTAA CATTTATGTG 
ACATACTGCA AAAACAAACC AGATTCCAGT CAGCTCATCC TAGACCATGC TGGGAGTTTT 
TTTGATGAGA TTCAGAAGAG ACACAGTCTG ACCAACTCTA TATCATCATA CCTCATCAAA 
CCTGTACAGA GGATCACCAA ATATCAGCTG CTGCTCAAGG AGCTGCTGTC CTGCTGTGAA 
GAAGGCAAAG GTGAAATTAA AGATGGCCTG GAGGTCATGC TGAGTGTGCC AAAGAGAGCC 
AATGACGCCA TGCACGTCAG CATGCTGGAG GGTTTTGATG CAAATTTGGA TGTTCAGGGT 
GAACTCATCC TCCAAGACAC CTTCCAGGTG TGGGACCCCA AATCACTAAT CCGGAAGGGG 
CGAGACCGCC ACCTCTTTCT CTTCGAGATC TCCCTGGTCT TCAGTAAAGA GATCAAAGAC 
TCAGCAGGGC GATCCAAATA CATCTTTAAA AACAAACTAC TGACTTCTGA ATTGGGGGTG 
ACAGAGCATG TAGAGGGGGA CCCGTGCAAA TTTGCTCTGT GGGTGGGACG CACACCATCG 
TCTGACAACA AGACTGTCCT CAAGGCATCT AGTATTGAGG TGAAGCAAGA ATGGATTAAA 
AACATCCGAG AGGTCATTCA GGAGAGAATA ATTCACCTGA AGGGGGCGCT AAAGGAACCT 
ATCCACATTC CCAAAACTCC AGCCAAACCT CGCAATAACA GCAAGAGGGA CGCTGGTGAG 
GATGCTGACA GCCAGGGAGA CGGCAGCAGT CAGCCGGACA CCATCTCCAT AGCATCCAGA 
ACCTCTCAGA ACACTGTAGA CAGTGACAAG CTCTCTGGAG GGTGTGAGCT GACGGTGGTC 
CTGCAGGACT TTACAGCAAG CAGCTCATCA GAGCTCAGCA TCCAGACGGG TCAGACAGTG 
GAGCTGCTGG AGCGGCCCAG TGACCGTCCA GGATGGTGTC TGGTACGGAC CACCGAAAAG 
ACGCCTCCTC AAGAGGGACT GGTGCCCAGC AGTGCTCTGT GTATCTCGCA CTCACGCAGC 
AGTGTGGAGA TGGACTGCTT TTTCCCAGCA GGCAAAGAAA ACTATACAGT TAGTGGCCCA 
GAAGGAAAGA CAGAGTCAGT GGCCACACTG CAGCCACAGC CATCCCTGAA CTCTCTGCAG 
AGTAGCTCTC CTGGACCCAA GCGCTCTGGG AATACACTGA AGAAATGGCT GACCAGTCCT 
GTCCGACGTC TAAGTCATGG CAGTAACATC AAGAAGATCC CAAGTAAACA GAAACAGAAA 
CGTGATGGTC GCAAGAGTAT TGATATGGGG CCGCCCGAAC TACAGGATGA CACCTTGGAA 
GAGCGAGTGC AAAAAGAAGA GGGCACACTC TCCAAGTCTT CGTCTGGGAT GCACAGTGGA 
GGAGAGGAAG AGCCTGAGGA CGAGTCTCAC ACTCCTCTAC CTCCACCAAT GGAGATCATT 
AAAGACCCGT CGGCTCAGGA AGAGAAGTCG TCTGTGCTTC TGGCTTCCCG GCAGAGCTCG 
GCTGATGTCC CCAGTGCTGC TGAGCTTGTC AGTGCTATAG AAAAACTGGT CAAAACTAAA 
ATGACACTGG AGCCAGGCTC ATACCCGGGA TTCAGCTCAG TCAATCCAGC AGAGCAGAGC 
CCGATTCCAC CCAGAAACCC AGAACTAGAG CAGAAAGCCA AAGCGCTACG AGGACGAATG 
TTTGTAGTCA AGGAATTAAT CCAAACGGAA AAAGACTATG TTAAAGATCT GGGAATTGTG 
GTTGAGGGGT TTATGAAGAG AATCGAGGAG AAAGGAGTTC CTGATAACAT GAAAGGAAAA 
GATAAGATCG TCTTCGGAAA CATTCACCAA ATCTATGACT GGCACAAAGA TTTTTTTGTT 
GGAGAACTGG AGAAATGCCT GGAAGATCAC GAAAAACTGC CTGAACTGTT CTGTAAACAT 
GAGAGACGGT TACACATGTA TGTAGTTTAC TGTCAGAACA AACCCAAATC CGAGTTCATC 
GTTGCAGAAT ATGATTCATA CTTTGAGGGG ATTCAGCAAG ACATTAACTC CAGACTGAGC 
ATCAGCGATT TTCTCATTAA ACCCATTCAG AGAATTACTA AATACCAGCT TTTATTAAAG 
GATGTTTTGA AGTACAGTAC AAAAGCAGGA CTGGATTGTC AGGATATAGA GAATGCAGTT 
GACCTGATGT CCCAGGTTCC CAAGCTTTGC AATGACATGA TGAATCTGGG AAGACTGCAG 
GGATATGAGG GGAAGTTGAC GAGTCAAGGA AAACTCTTGC AGCAGGAGAC GTTCTTTGTG 
ACCGAGCAGG ACTCTGGCGT TCTGTCCCGC AGTAAAGAGC GCAGGGTTTT CCTGTTCGAA 
CAAATTGTCA TCTTCAGCGA GCTGTTACGT AAAGGCTCTT CCACTCCAGG GTACCAGTTC 
AAGAAGAGCA TAAAGGTGAG CTTCCTTGAC TTGGAAGAGC ATGTTGAAAA TGATCCATGC 
AAGTTTGTGC TGTCGTGTCG TGGGTCGGCA GAACGTTTTA CCCTTCAAGC AGCAAACACG 
GACATTAAAC AAGTCTGGGT TCAGCATATC ACCCAACTAC TAGATTTACA AAGCAACTTC 
TTGTCAGCTC TGCAGTCTCC TATTGAGTAT CAGAAGGAGC GTAGTGGCTC TCAATCTCTG 
ACCCGGAACT CTTCTGGGCG GATGGGTCAG TCCAACAGCA GGCCCTGCTC CACGGTTTCA 
CTCGGAGCAG AGAAACCCTC TCTGTCTGGA CGGAGCTCCA CCCCGCTAAA GCTATCTACC 
TCCAATGGTG GTCCATGCTC TGAAAGATAC CGCAGGCAGT TTGAAGGCTT GGGCTGCAAC 
GGTACATCGT CCTCCTTGAT GGTGACTCAA GATTATACTG CACTGAAAGA GAATGAGATC 
TGTGTCACAC AGGGTGAGGC CGTGCAGGTA CTGGCCACTA ACCAGCAGAA CATGTACCTT 
GTGTACCGAC CCGCAAATAG CCAATCACCA CCTGCAGAGG GCTGGGTGCC GGGACACATC 
CTAGGCCCCG TCACAAAACC TCTCATAGAC ACTACTGCAG ACGCAAGCAT CAAGAAATCT 
CTTTCGTGGA ACACACTTCG TGCTCGTAAG CGTGCAGAAA AGGACAGCAC GCCCCTCAAA 
AGTGACTTTA AAGTTGAGAA TGGACTGCGT AAGCCAAAGG AAATTCTCAG CAGCAAAGTC 
AAAGTGAAAG AGTCCCGCAG TTCTGATGAG TCTGAATGTG AGGATGAACT AGACCCCAAA 
ACTAGTATGG AGTTACTGAA CCCTAATTTC ATCCAAGAGG TCGCTCCAGA GTTTCTGACG 
CCTCTAACAG ACGTGACGTG TGCTCTGGGA GAGACGGTGG TTCTGTGCTG TAAAGTGTGT 
GCTCGACCCA AACCCACAAT CACCTTGAAA GGACCTGATC AAAGTCTGCT GGCCAACAAT 
AACCGCTTCA CTATTAATAT CAGAGAAACC GGTGATATAG TGTTGAAAAT CTGCAATCTA 
ATGCCGCAGG ACACTGGCAT CTACACGTGT GTTGCTGTGA ATGACCATGG TACAGCCTCC 
TCATCTGCAT CCATTAAAGT ACAAGGTATC CCAGCAGCCC CTGCTCGACC GGTGGCACAG 
GAGGCAAGCA GCACTGCAGT GATTGTCCAC TGGCTTCCTC CAGCCAGCTC AGCAAATTCT 
GCCATCTCCA GCTACACTGT GGAATATAGG CAGGAAGATT CTCTGGTATG GCAGCAGTTG 
GTGGTCTCCA CGGCAGATGT GTTTGTGAAG ATTGACAATC TGATTCCTGG TGGGCACTAC 
CAGTTCAGAG TCAGTGCCAG TAATCCCTGG GGCACCAGCC CGCCTAGTGA ACCCTCAAAC 
ATGGTGACAT TATCCAGCAC AGCATCAACA TATGATGGCG CTGGAATCCA GTGGAAAGAC 
AACTTTGAAT CTGCTTTTAC AGAATTATGT GAAATTGGGC GGGGTCGTTT CTCTGTGGTG 
AGGAAGTGTC TCAGTAAAGC CACTAAGCAG GAGGTTGCTG TGAAGTTTGT GAATAAGAAG 
ATGCACAAGA AAGAACAGGT GGCCCATGAA GCTGATATTC TACGCCATGT CCAGCACCCC 
CAACTGGTGT CACTGATTGA TACATACGAG TCGCCTACAG CCTACATGCT GATTCTTGAA 
CTTGTTGAGG ATGGACGTCT TCTTGACTAT CTGGTAGCTC ATGATGAGCT GATGGAGGAG 
AAAGTGGCAT TCTTTATTAA AGATACACTG GAAGCTCTGC AGCACTTGCA CACCTGCAGA 
GTGGCTCATC TAGACCTCAA GCCTGAAAAC TTGTTGGTGG ACCTCCACGT TCCGGTACCC 
TGCGTGAAGC TCTCAGATCT AGGTGATGCT GTACAGGTTT CAAGTCACCG CTACGTACAT 
CTCCTCCTGG GTAACCCCGA GTTCGCTGCT CCAGAGCTCA TCCAGGGCAC TCCAGTGTCC 
CTCTCTACAG ATGTGTGGAG TGTAGGGGTG CTGGCCTACG TCATGCTTAG TGGAGTCTCC 
CCATTCCTGG ATGAGAGTCT GGAGGAGACC TGCGTCAACA TCTGCAGGCT GGACTTCTGC 
TTTCCTCAAG AGTACTTCTC CGATATAAGC CAGGCAGCCA AAGACTTCAT TGTGTCCACG 
CTTAACCAGG ATCCCAGGAA GAGGCCAAGC TCGGCCTCGT GTCTTCAGCA CCCATGGGTC 
AGTGCTCATA GTGGAGATTA CTCCAAAACA CCTCTGGATA CTGTCAGACT CGCTGCATTT 
ATAGACAGAC GCAAACAGCT ACATGATGTC AGGCCTGTCA CAAACATTAA AGGAATGGTC 
AGCAGCAGTA TGGGACACAC CTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kalrna ( XM_017357741.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213230.1 Danio rerio kalirin, RhoGEF kinase a (kalrna), transcript variant X1, mRNA.

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CloneID	ODa60287
Clone ID Related Accession (Same CDS sequence)	XM_017357741.2 , XM_017357741.1
Accession Version	XM_017357741.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8907bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kalirin isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTG CTGAAGCGCC GGAGGACGGA CCCAATGATT CGGACACGGA TGCATTCTTC 
AAAACAGGGT CTTTCAGAAG TGAAGGAATT AAAGCCTCTG ATGTCCTTCC TGTGCTGAGA 
GAAAAAATAG CATTTGTGTC AGGCGGTCGT GATAAGCGAG GTGGCCCTAT ACTGACCTTT 
CCTGCAAGGA GTAACCATGA CCGAATAAAA CAAGAGGACC TGAGACGGCT CGTAACATAC 
CTGTCCACTG TGCCCAGTGA GGACGTTTGT AAGAGAGGCT TCACTGTGAT TATTGATATG 
CGTGGCTCTA AATGGGAGCT AATAAAGCCA CTGCTGAAGA CGCTACAGGA AGCATTTCCA 
GCTGAGATCT GCGTCGCTCT CATCATCAAA CCAGACAACT TCTGGCAGAA GCAGAAGACT 
AACTTTGGTA GCGCCAAGTT CACCTTTGAG ACTAGCATGG TATCAGTGGA GGGCTTGACC 
AAACTAGTGG ACCCATCCCA GCTAACGGAC GACTTTGAAG GTTCTCTGGA ATACAATCAT 
GAGGAATGGC TTGAGCTCCG GGTGGCTCTG GAGGAGTTCC TGGCCAGCGC CGTTCACCTC 
CTCTCTCGCC TGGAAGATCT ACAGGAGACT CTGGCTAAGA AGGAGTTCCC GGTGGATGTG 
GAAGCCTCTC GTAGACTCAT CGATGAGCAC ACACAGCTGA AGAAGAAGGT GATGAAGGCT 
CCGGTGGAAG AGCTGGACCA CGAAGGCCAG AGACTCTTGC AGTGCATCCG CTCCAGCGAC 
GGCTTCTCAG GGAGGAACTG CATTTCTGGA AGTGCCGACT TCCAGAGCGT GGTTCCGAAG 
ATCGCCAGTC TGCTGGATAA ACTCCACAGC ACTCGACAGC ACTTGCACCA GATGTGGCAC 
CTACGAAAAC TCAAGCTGGA TCAGTGCTTT CAGCTCCGAC TGTTTGAACA GGACGCAGAG 
AAGATGTTTG ACTGGATCAG TCACAATAAG GAATTGTTTC TTCAGAGCCA CACGGAGATC 
GGTGTGAGCT ACCAGCATGC TGTGGAGCTA CAGACGCAAC ACAACCACTT TGCCATGAAC 
TCAATGAATG CCTATGTGAA CATAAACCGC ATCATGTCGG TGGCGTCGCG GCTGTCGGAG 
GCGGGCCACT ACGCCGCTCA GCAGATCAAA CAGATCAGCA CTCAGCTGGA TCAGGAGTGG 
AAGAGCTTCG CGGCTGCTCT CGATGAACGC AGCACCATCC TTGCCATGTC TGCCGTCTTC 
CATCAGAAAT CTGAACAGTT TTTGTCCGGT GTGGAAACAT GGTGTAAGAT GTGCAGTGAA 
GGCGGCTTGC CATCAGAGAT GCAGGATCTG GAATCGGCCA TTCATCACCA TCAGACGCTC 
TATGAACAAG TCACACAAGC ATACACCGAG GTGAGTCAGG ACGGTAAGGC TTTGCTGGAC 
GTTCTTCAGC GGCCGTTGAG TCCTGGGAAC GCAGAGTCAC TGACTGCGAC AGCGAACTAC 
TCTAAAGCCG TGCACTGTAT ACTGGACGTG GTCCACGAGG TCCTGCATCA TCAGAGACGT 
CTGGAGAACA TCTGGCAGCA CCGTAAAGTC CGTCTGCATC AGAGACTGCA GCTCTGTGTC 
TTCCAGCAGG ACGTCCAACA GGTGATTGAC TGGATAGAGA ATCACGGGGA GGCTTTCCTC 
AGCAAACACA CTGGTGTGGG CAAATCCCTG CACAGAGCCA GAGCCCTCCA GAAACGCCAT 
GATGACTTTG AGGAGGTGGC ACAGAATACA TACACTAATG CAGATAAGCT GCTGGAAGCT 
GCTGAGCAGT TGGCTCAGAC GGGCGAGTGT GACCCTGAGG AGATCTACAA GGCAGCGCGA 
CACCTGGAAG TCAGGATCCA GGACTTTGTT AGGCGAGTGG AGCAAAGAAA ACTACTGCTG 
GACATGTCTG TCTCTTTCCA CACGCACACT AAAGAGTTGT GGACGTGGAT GGAGGACCTG 
CAGAAAGAGC TGCTGGAGGA AGTTTGTTCT GACTCAGTCG ATTCAGTTCA GGAGCTGATA 
AAACAGTTTC AGCAGCAGCA GACGGCCACG CTGGAGGCAA CACTCAATGT CATTAAAGAG 
GGAGAAGATC TTATGCAACA ACTCCGAGAT TCAGCAATGA GCAGTAATAA GATGCCCCAC 
ACCAGCTCCA TCGCTCACAT TGAGGGCGTC CTGCAGCAGC TAGAGGAGGC GCAGGGCCAC 
ATGGAGGAAC TCTTCCATGA GAGAAAGATC AAACTCGACA TCTTCCTGCA ACTGCGCATC 
TTTGAGCAGT ACACCATTGA GGTAACGGCA GAGTTAGACG CGTGGAATGA AGACTTGGTT 
CGTCAGATGA ATGAGTATAC TGGAGAAGAA CCCAGTCTGG CTGAGCAGAG ACTCCAGCGG 
CACGCAGAGA GGAAACTGGC CATGACTAAC ATGACCTACG AAGTTATGCA GCAGGGTCAG 
GACCTGCATC AGTACATCAT GGAAGTCCAA GCTTCAGGTA TTGAGTTGAC CGGAGAGAAG 
GAGGTGGATC TGGCAACACA AGTTCAGGAG CTGCTGGAGT TCCTTCATGA GAAACAGCAC 
GAGTTGGACA TGAGCGCCGA ACACACACAG AAGCGCCTGG AGCAGTGTGT GCAGCTCCGC 
CACCTGCAGG CTGAGGTTAA ACAGGTTCTG GGGTGGATTC GTAATGGTGA ATCTATGTTG 
GATGCCAGTA TGGTGAATGC AAGTTCACTG TCAGAGGCCG AACAACTACA GAGAGAACAT 
GAACAGTTCC AGCTGGCCAT AGAGTCTTTG TTCCACGCTA ACTCTCTCCA GAAAACCCAT 
CAGAGTGCAC TACAGGTCCA GCAGAAGGCA GAAATCATGT TACAGGCTGG ACACTTTGAC 
CCCGAGGCCA TCCGCAGCTG TGCAGAGAAG GTGGCCGTGC ACTGGCAGCA GCTCATGCTC 
AAGATGGAGG ACAGACTCAA ACTGGTTAAT GCCTCGGTGG CCTTCTACAA GACGTCAGAA 
CAGGTGTGTA GTGTCCTGGA GAGTCTGGAG CAGGAGTACC GCAGAGATGA AGACTGGTGT 
GGAGCTCATG ATAAACTAGG ATCTTCATCA GAGACTGATC ACGTTCTGCC TCTCATCAGC 
AAACACCTGG AGCAGAAAGA AGCTTTTCTC AAGGCATGCA CTCTGGCCAG GCGCAATGCT 
GAGGTTTTTC TGAAATACAT CCACCGGAAC AATGTCAGCT CTCAGGGATC ATCTAGCAAC 
ACTCGTGGCC CTGAGCAACA GGTCAAAGCC ATCCTGAATG AACTGCTGCA GAGGGAAAAC 
AGAGTTTTGC ATTTCTGGAC CATGAAGAAG CGGCGTTTGG ATCAGTGTCA GCAGTATGTG 
GTGTTTGAAC GCAATGCCAA ACAGGCATTA GAGTGGATTG AGGAGGCAGG AGAGGTCTAT 
CTGGCCACTC ACACCTGCAT CGGAGACTCT ATGGAGAAAA CTCAGGGACT CCTCAAAGAA 
TACGAAGAAT TCTGCATCTC CGCAAAGCAA ACTAAGGAGA AAGTGAAGAT GCTGATCCAG 
CTGGCGGATA ACTTTGTTGA GAAAGGACAC ATGCATGTCA CCGACTTGAA GAAGTGTCTG 
ACGGCAGTGG ACAAGCGCTA CAGGGACTTC TCCATGCGCA TGGGCAAATA CCGCTGTGAC 
CTGGAAAATG TGCTGGGCAT CAGCTCTGAG GATAATAAGG ATCTGGAGCT GGATATCATC 
CCAGCCAGTC TGACTGGCCC AGAGGTTAAA CTCCGTGACC CCAACCACGA GGTGAATGAA 
GAAAAGAGGA AATCAGCCCG GAAGAAAGAG TTTATTATAG CAGAGCTGTT GCAGACAGAA 
AAGGCTTATG TGAGGGACTT ACATGAATGC TTAGAGACAT ATTTGTGGGA GATGACCAAT 
GGTGTGGAGG AAATTCCTCC AGGAATCGCC AACAAAGAGC ACATCATCTT TGGCAACATT 
CAGGAGATTT ACGACTTCCA CAATAATATA TTCCTGAAGG AGCTGATCAA TTATGAGCAA 
CTGCCTGAGG ATGTGGGACA TTGCTTTGTG ACATGGGCCG ATAAGTTTAA CATTTATGTG 
ACATACTGCA AAAACAAACC AGATTCCAGT CAGCTCATCC TAGACCATGC TGGGAGTTTT 
TTTGATGAGA TTCAGAAGAG ACACAGTCTG ACCAACTCTA TATCATCATA CCTCATCAAA 
CCTGTACAGA GGATCACCAA ATATCAGCTG CTGCTCAAGG AGCTGCTGTC CTGCTGTGAA 
GAAGGCAAAG GTGAAATTAA AGATGGCCTG GAGGTCATGC TGAGTGTGCC AAAGAGAGCC 
AATGACGCCA TGCACGTCAG CATGCTGGAG GGTTTTGATG CAAATTTGGA TGTTCAGGGT 
GAACTCATCC TCCAAGACAC CTTCCAGGTG TGGGACCCCA AATCACTAAT CCGGAAGGGG 
CGAGACCGCC ACCTCTTTCT CTTCGAGATC TCCCTGGTCT TCAGTAAAGA GATCAAAGAC 
TCAGCAGGGC GATCCAAATA CATCTTTAAA AACAAACTAC TGACTTCTGA ATTGGGGGTG 
ACAGAGCATG TAGAGGGGGA CCCGTGCAAA TTTGCTCTGT GGGTGGGACG CACACCATCG 
TCTGACAACA AGACTGTCCT CAAGGCATCT AGTATTGAGG TGAAGCAAGA ATGGATTAAA 
AACATCCGAG AGGTCATTCA GGAGAGAATA ATTCACCTGA AGGGGGCGCT AAAGGAACCT 
ATCCACATTC CCAAAACTCC AGCCAAACCT CGCAATAACA GCAAGAGGGA CGCTGGTGAG 
GATGCTGACA GCCAGGGAGA CGGCAGCAGT CAGCCGGACA CCATCTCCAT AGCATCCAGA 
ACCTCTCAGA ACACTGTAGA CAGTGACAAG CTCTCTGGAG GGTGTGAGCT GACGGTGGTC 
CTGCAGGACT TTACAGCAAG CAGCTCATCA GAGCTCAGCA TCCAGACGGG TCAGACAGTG 
GAGCTGCTGG AGCGGCCCAG TGACCGTCCA GGATGGTGTC TGGTACGGAC CACCGAAAAG 
ACGCCTCCTC AAGAGGGACT GGTGCCCAGC AGTGCTCTGT GTATCTCGCA CTCACGCAGC 
AGTGTGGAGA TGGACTGCTT TTTCCCAGCA GGCAAAGAAA ACTATACAGT TAGTGGCCCA 
GAAGGAAAGA CAGAGTCAGT GGCCACACTG CAGCCACAGC CATCCCTGAA CTCTCTGCAG 
AGTAGCTCTC CTGGACCCAA GCGCTCTGGG AATACACTGA AGAAATGGCT GACCAGTCCT 
GTCCGACGTC TAAGTCATGG CAGTAACATC AAGAAGATCC CAAGTAAACA GAAACAGAAA 
CGTGATGGTC GCAAGAGTAT TGATATGGGG CCGCCCGAAC TACAGGATGA CACCTTGGAA 
GAGCGAGTGC AAAAAGAAGA GGGCACACTC TCCAAGTCTT CGTCTGGGAT GCACAGTGGA 
GGAGAGGAAG AGCCTGAGGA CGAGTCTCAC ACTCCTCTAC CTCCACCAAT GGAGATCATT 
AAAGACCCGT CGGCTCAGGA AGAGAAGTCG TCTGTGCTTC TGGCTTCCCG GCAGAGCTCG 
GCTGATGTCC CCAGTGCTGC TGAGCTTGTC AGTGCTATAG AAAAACTGGT CAAAACTAAA 
ATGACACTGG AGCCAGGCTC ATACCCGGGA TTCAGCTCAG TCAATCCAGC AGAGCAGAGC 
CCGATTCCAC CCAGAAACCC AGAACTAGAG CAGAAAGCCA AAGCGCTACG AGGACGAATG 
TTTGTAGTCA AGGAATTAAT CCAAACGGAA AAAGACTATG TTAAAGATCT GGGAATTGTG 
GTTGAGGGGT TTATGAAGAG AATCGAGGAG AAAGGAGTTC CTGATAACAT GAAAGGAAAA 
GATAAGATCG TCTTCGGAAA CATTCACCAA ATCTATGACT GGCACAAAGA TTTTTTTGTT 
GGAGAACTGG AGAAATGCCT GGAAGATCAC GAAAAACTGC CTGAACTGTT CTGTAAACAT 
GAGAGACGGT TACACATGTA TGTAGTTTAC TGTCAGAACA AACCCAAATC CGAGTTCATC 
GTTGCAGAAT ATGATTCATA CTTTGAGGGG ATTCAGCAAG ACATTAACTC CAGACTGAGC 
ATCAGCGATT TTCTCATTAA ACCCATTCAG AGAATTACTA AATACCAGCT TTTATTAAAG 
GATGTTTTGA AGTACAGTAC AAAAGCAGGA CTGGATTGTC AGGATATAGA GAATGCAGTT 
GACCTGATGT CCCAGGTTCC CAAGCTTTGC AATGACATGA TGAATCTGGG AAGACTGCAG 
GGATATGAGG GGAAGTTGAC GAGTCAAGGA AAACTCTTGC AGCAGGAGAC GTTCTTTGTG 
ACCGAGCAGG ACTCTGGCGT TCTGTCCCGC AGTAAAGAGC GCAGGGTTTT CCTGTTCGAA 
CAAATTGTCA TCTTCAGCGA GCTGTTACGT AAAGGCTCTT CCACTCCAGG GTACCAGTTC 
AAGAAGAGCA TAAAGGTGAG CTTCCTTGAC TTGGAAGAGC ATGTTGAAAA TGATCCATGC 
AAGTTTGTGC TGTCGTGTCG TGGGTCGGCA GAACGTTTTA CCCTTCAAGC AGCAAACACG 
GACATTAAAC AAGTCTGGGT TCAGCATATC ACCCAACTAC TAGATTTACA AAGCAACTTC 
TTGTCAGCTC TGCAGTCTCC TATTGAGTAT CAGAAGGAGC GTAGTGGCTC TCAATCTCTG 
ACCCGGAACT CTTCTGGGCG GATGGGTCAG TCCAACAGCA GGCCCTGCTC CACGGTTTCA 
CTCGGAGCAG AGAAACCCTC TCTGTCTGGA CGGAGCTCCA CCCCGCTAAA GCTATCTACC 
TCCAATGGTG GTCCATGCTC TGAAAGATAC CGCAGGCAGT TTGAAGGCTT GGGCTGCAAC 
GGTACATCGT CCTCCTTGAT GGTGACTCAA GATTATACTG CACTGAAAGA GAATGAGATC 
TGTGTCACAC AGGGTGAGGC CGTGCAGGTA CTGGCCACTA ACCAGCAGAA CATGTACCTT 
GTGTACCGAC CCGCAAATAG CCAATCACCA CCTGCAGAGG GCTGGGTGCC GGGACACATC 
CTAGGCCCCG TCACAAAACC TCTCATAGAC ACTACTGCAG ACGCAAGCAT CAAGAAATCT 
CTTTCGTGGA ACACACTTCG TGCTCGTAAG CGTGCAGAAA AGGACAGCAC GCCCCTCAAA 
AGTGACTTTA AAGTTGAGAA TGGACTGCGT AAGCCAAAGG AAATTCTCAG CAGCAAAGTC 
AAAGTGAAAG AGTCCCGCAG TTCTGATGAG TCTGAATGTG AGGATGAACT AGACCCCAAA 
ACTAGTATGG AGTTACTGAA CCCTAATTTC ATCCAAGAGG TCGCTCCAGA GTTTCTGACG 
CCTCTAACAG ACGTGACGTG TGCTCTGGGA GAGACGGTGG TTCTGTGCTG TAAAGTGTGT 
GCTCGACCCA AACCCACAAT CACCTTGAAA GGACCTGATC AAAGTCTGCT GGCCAACAAT 
AACCGCTTCA CTATTAATAT CAGAGAAACC GGTGATATAG TGTTGAAAAT CTGCAATCTA 
ATGCCGCAGG ACACTGGCAT CTACACGTGT GTTGCTGTGA ATGACCATGG TACAGCCTCC 
TCATCTGCAT CCATTAAAGT ACAAGGTATC CCAGCAGCCC CTGCTCGACC GGTGGCACAG 
GAGGCAAGCA GCACTGCAGT GATTGTCCAC TGGCTTCCTC CAGCCAGCTC AGCAAATTCT 
GCCATCTCCA GCTACACTGT GGAATATAGG CAGGAAGATT CTCTGGTATG GCAGCAGTTG 
GTGGTCTCCA CGGCAGATGT GTTTGTGAAG ATTGACAATC TGATTCCTGG TGGGCACTAC 
CAGTTCAGAG TCAGTGCCAG TAATCCCTGG GGCACCAGCC CGCCTAGTGA ACCCTCAAAC 
ATGGTGACAT TATCCAGCAC AGCATCAACA TATGATGGCG CTGGAATCCA GTGGAAAGAC 
AACTTTGAAT CTGCTTTTAC AGAATTATGT GAAATTGGGC GGGGTCGTTT CTCTGTGGTG 
AGGAAGTGTC TCAGTAAAGC CACTAAGCAG GAGGTTGCTG TGAAGTTTGT GAATAAGAAG 
ATGCACAAGA AAGAACAGGT GGCCCATGAA GCTGATATTC TACGCCATGT CCAGCACCCC 
CAACTGGTGT CACTGATTGA TACATACGAG TCGCCTACAG CCTACATGCT GATTCTTGAA 
CTTGTTGAGG ATGGACGTCT TCTTGACTAT CTGGTAGCTC ATGATGAGCT GATGGAGGAG 
AAAGTGGCAT TCTTTATTAA AGATACACTG GAAGCTCTGC AGCACTTGCA CACCTGCAGA 
GTGGCTCATC TAGACCTCAA GCCTGAAAAC TTGTTGGTGG ACCTCCACGT TCCGGTACCC 
TGCGTGAAGC TCTCAGATCT AGGTGATGCT GTACAGGTTT CAAGTCACCG CTACGTACAT 
CTCCTCCTGG GTAACCCCGA GTTCGCTGCT CCAGAGCTCA TCCAGGGCAC TCCAGTGTCC 
CTCTCTACAG ATGTGTGGAG TGTAGGGGTG CTGGCCTACG TCATGCTTAG TGGAGTCTCC 
CCATTCCTGG ATGAGAGTCT GGAGGAGACC TGCGTCAACA TCTGCAGGCT GGACTTCTGC 
TTTCCTCAAG AGTACTTCTC CGATATAAGC CAGGCAGCCA AAGACTTCAT TGTGTCCACG 
CTTAACCAGG ATCCCAGGAA GAGGCCAAGC TCGGCCTCGT GTCTTCAGCA CCCATGGGTC 
AGTGCTCATA GTGGAGATTA CTCCAAAACA CCTCTGGATA CTGTCAGACT CGCTGCATTT 
ATAGACAGAC GCAAACAGCT ACATGATGTC AGGCCTGTCA CAAACATTAA AGGAATGGTC 
AGCAGCAGTA TGGGACACAC CTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213230.1
CDS	237..9143
Translation	MNSAEAPEDGPNDSDTDAFFKTGSFRSEGIKASDVLPVLREKIAFVSGGRDKRGGPILTFPARSNHDRIKQEDLRRLVTYLSTVPSEDVCKRGFTVIIDMRGSKWELIKPLLKTLQEAFPAEICVALIIKPDNFWQKQKTNFGSAKFTFETSMVSVEGLTKLVDPSQLTDDFEGSLEYNHEEWLELRVALEEFLASAVHLLSRLEDLQETLAKKEFPVDVEASRRLIDEHTQLKKKVMKAPVEELDHEGQRLLQCIRSSDGFSGRNCISGSADFQSVVPKIASLLDKLHSTRQHLHQMWHLRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHAVELQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYAAQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKSEQFLSGVETWCKMCSEGGLPSEMQDLESAIHHHQTLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNAESLTATANYSKAVHCILDVVHEVLHHQRRLENIWQHRKVRLHQRLQLCVFQQDVQQVIDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKELLEEVCSDSVDSVQELIKQFQQQQTATLEATLNVIKEGEDLMQQLRDSAMSSNKMPHTSSIAHIEGVLQQLEEAQGHMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLVRQMNEYTGEEPSLAEQRLQRHAERKLAMTNMTYEVMQQGQDLHQYIMEVQASGIELTGEKEVDLATQVQELLEFLHEKQHELDMSAEHTQKRLEQCVQLRHLQAEVKQVLGWIRNGESMLDASMVNASSLSEAEQLQREHEQFQLAIESLFHANSLQKTHQSALQVQQKAEIMLQAGHFDPEAIRSCAEKVAVHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGAHDKLGSSSETDHVLPLISKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSSQGSSSNTRGPEQQVKAILNELLQRENRVLHFWTMKKRRLDQCQQYVVFERNAKQALEWIEEAGEVYLATHTCIGDSMEKTQGLLKEYEEFCISAKQTKEKVKMLIQLADNFVEKGHMHVTDLKKCLTAVDKRYRDFSMRMGKYRCDLENVLGISSEDNKDLELDIIPASLTGPEVKLRDPNHEVNEEKRKSARKKEFIIAELLQTEKAYVRDLHECLETYLWEMTNGVEEIPPGIANKEHIIFGNIQEIYDFHNNIFLKELINYEQLPEDVGHCFVTWADKFNIYVTYCKNKPDSSQLILDHAGSFFDEIQKRHSLTNSISSYLIKPVQRITKYQLLLKELLSCCEEGKGEIKDGLEVMLSVPKRANDAMHVSMLEGFDANLDVQGELILQDTFQVWDPKSLIRKGRDRHLFLFEISLVFSKEIKDSAGRSKYIFKNKLLTSELGVTEHVEGDPCKFALWVGRTPSSDNKTVLKASSIEVKQEWIKNIREVIQERIIHLKGALKEPIHIPKTPAKPRNNSKRDAGEDADSQGDGSSQPDTISIASRTSQNTVDSDKLSGGCELTVVLQDFTASSSSELSIQTGQTVELLERPSDRPGWCLVRTTEKTPPQEGLVPSSALCISHSRSSVEMDCFFPAGKENYTVSGPEGKTESVATLQPQPSLNSLQSSSPGPKRSGNTLKKWLTSPVRRLSHGSNIKKIPSKQKQKRDGRKSIDMGPPELQDDTLEERVQKEEGTLSKSSSGMHSGGEEEPEDESHTPLPPPMEIIKDPSAQEEKSSVLLASRQSSADVPSAAELVSAIEKLVKTKMTLEPGSYPGFSSVNPAEQSPIPPRNPELEQKAKALRGRMFVVKELIQTEKDYVKDLGIVVEGFMKRIEEKGVPDNMKGKDKIVFGNIHQIYDWHKDFFVGELEKCLEDHEKLPELFCKHERRLHMYVVYCQNKPKSEFIVAEYDSYFEGIQQDINSRLSISDFLIKPIQRITKYQLLLKDVLKYSTKAGLDCQDIENAVDLMSQVPKLCNDMMNLGRLQGYEGKLTSQGKLLQQETFFVTEQDSGVLSRSKERRVFLFEQIVIFSELLRKGSSTPGYQFKKSIKVSFLDLEEHVENDPCKFVLSCRGSAERFTLQAANTDIKQVWVQHITQLLDLQSNFLSALQSPIEYQKERSGSQSLTRNSSGRMGQSNSRPCSTVSLGAEKPSLSGRSSTPLKLSTSNGGPCSERYRRQFEGLGCNGTSSSLMVTQDYTALKENEICVTQGEAVQVLATNQQNMYLVYRPANSQSPPAEGWVPGHILGPVTKPLIDTTADASIKKSLSWNTLRARKRAEKDSTPLKSDFKVENGLRKPKEILSSKVKVKESRSSDESECEDELDPKTSMELLNPNFIQEVAPEFLTPLTDVTCALGETVVLCCKVCARPKPTITLKGPDQSLLANNNRFTINIRETGDIVLKICNLMPQDTGIYTCVAVNDHGTASSSASIKVQGIPAAPARPVAQEASSTAVIVHWLPPASSANSAISSYTVEYRQEDSLVWQQLVVSTADVFVKIDNLIPGGHYQFRVSASNPWGTSPPSEPSNMVTLSSTASTYDGAGIQWKDNFESAFTELCEIGRGRFSVVRKCLSKATKQEVAVKFVNKKMHKKEQVAHEADILRHVQHPQLVSLIDTYESPTAYMLILELVEDGRLLDYLVAHDELMEEKVAFFIKDTLEALQHLHTCRVAHLDLKPENLLVDLHVPVPCVKLSDLGDAVQVSSHRYVHLLLGNPEFAAPELIQGTPVSLSTDVWSVGVLAYVMLSGVSPFLDESLEETCVNICRLDFCFPQEYFSDISQAAKDFIVSTLNQDPRKRPSSASCLQHPWVSAHSGDYSKTPLDTVRLAAFIDRRKQLHDVRPVTNIKGMVSSSMGHTL

Target ORF information:

RefSeq Version	XM_017357741.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kalirin, RhoGEF kinase a (kalrna), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017357741.1

ORF Insert Sequence:

ATGAATTCTG CTGAAGCGCC GGAGGACGGA CCCAATGATT CGGACACGGA TGCATTCTTC 
AAAACAGGGT CTTTCAGAAG TGAAGGAATT AAAGCCTCTG ATGTCCTTCC TGTGCTGAGA 
GAAAAAATAG CATTTGTGTC AGGCGGTCGT GATAAGCGAG GTGGCCCTAT ACTGACCTTT 
CCTGCAAGGA GTAACCATGA CCGAATAAAA CAAGAGGACC TGAGACGGCT CGTAACATAC 
CTGTCCACTG TGCCCAGTGA GGACGTTTGT AAGAGAGGCT TCACTGTGAT TATTGATATG 
CGTGGCTCTA AATGGGAGCT AATAAAGCCA CTGCTGAAGA CGCTACAGGA AGCATTTCCA 
GCTGAGATCT GCGTCGCTCT CATCATCAAA CCAGACAACT TCTGGCAGAA GCAGAAGACT 
AACTTTGGTA GCGCCAAGTT CACCTTTGAG ACTAGCATGG TATCAGTGGA GGGCTTGACC 
AAACTAGTGG ACCCATCCCA GCTAACGGAC GACTTTGAAG GTTCTCTGGA ATACAATCAT 
GAGGAATGGC TTGAGCTCCG GGTGGCTCTG GAGGAGTTCC TGGCCAGCGC CGTTCACCTC 
CTCTCTCGCC TGGAAGATCT ACAGGAGACT CTGGCTAAGA AGGAGTTCCC GGTGGATGTG 
GAAGCCTCTC GTAGACTCAT CGATGAGCAC ACACAGCTGA AGAAGAAGGT GATGAAGGCT 
CCGGTGGAAG AGCTGGACCA CGAAGGCCAG AGACTCTTGC AGTGCATCCG CTCCAGCGAC 
GGCTTCTCAG GGAGGAACTG CATTTCTGGA AGTGCCGACT TCCAGAGCGT GGTTCCGAAG 
ATCGCCAGTC TGCTGGATAA ACTCCACAGC ACTCGACAGC ACTTGCACCA GATGTGGCAC 
CTACGAAAAC TCAAGCTGGA TCAGTGCTTT CAGCTCCGAC TGTTTGAACA GGACGCAGAG 
AAGATGTTTG ACTGGATCAG TCACAATAAG GAATTGTTTC TTCAGAGCCA CACGGAGATC 
GGTGTGAGCT ACCAGCATGC TGTGGAGCTA CAGACGCAAC ACAACCACTT TGCCATGAAC 
TCAATGAATG CCTATGTGAA CATAAACCGC ATCATGTCGG TGGCGTCGCG GCTGTCGGAG 
GCGGGCCACT ACGCCGCTCA GCAGATCAAA CAGATCAGCA CTCAGCTGGA TCAGGAGTGG 
AAGAGCTTCG CGGCTGCTCT CGATGAACGC AGCACCATCC TTGCCATGTC TGCCGTCTTC 
CATCAGAAAT CTGAACAGTT TTTGTCCGGT GTGGAAACAT GGTGTAAGAT GTGCAGTGAA 
GGCGGCTTGC CATCAGAGAT GCAGGATCTG GAATCGGCCA TTCATCACCA TCAGACGCTC 
TATGAACAAG TCACACAAGC ATACACCGAG GTGAGTCAGG ACGGTAAGGC TTTGCTGGAC 
GTTCTTCAGC GGCCGTTGAG TCCTGGGAAC GCAGAGTCAC TGACTGCGAC AGCGAACTAC 
TCTAAAGCCG TGCACTGTAT ACTGGACGTG GTCCACGAGG TCCTGCATCA TCAGAGACGT 
CTGGAGAACA TCTGGCAGCA CCGTAAAGTC CGTCTGCATC AGAGACTGCA GCTCTGTGTC 
TTCCAGCAGG ACGTCCAACA GGTGATTGAC TGGATAGAGA ATCACGGGGA GGCTTTCCTC 
AGCAAACACA CTGGTGTGGG CAAATCCCTG CACAGAGCCA GAGCCCTCCA GAAACGCCAT 
GATGACTTTG AGGAGGTGGC ACAGAATACA TACACTAATG CAGATAAGCT GCTGGAAGCT 
GCTGAGCAGT TGGCTCAGAC GGGCGAGTGT GACCCTGAGG AGATCTACAA GGCAGCGCGA 
CACCTGGAAG TCAGGATCCA GGACTTTGTT AGGCGAGTGG AGCAAAGAAA ACTACTGCTG 
GACATGTCTG TCTCTTTCCA CACGCACACT AAAGAGTTGT GGACGTGGAT GGAGGACCTG 
CAGAAAGAGC TGCTGGAGGA AGTTTGTTCT GACTCAGTCG ATTCAGTTCA GGAGCTGATA 
AAACAGTTTC AGCAGCAGCA GACGGCCACG CTGGAGGCAA CACTCAATGT CATTAAAGAG 
GGAGAAGATC TTATGCAACA ACTCCGAGAT TCAGCAATGA GCAGTAATAA GATGCCCCAC 
ACCAGCTCCA TCGCTCACAT TGAGGGCGTC CTGCAGCAGC TAGAGGAGGC GCAGGGCCAC 
ATGGAGGAAC TCTTCCATGA GAGAAAGATC AAACTCGACA TCTTCCTGCA ACTGCGCATC 
TTTGAGCAGT ACACCATTGA GGTAACGGCA GAGTTAGACG CGTGGAATGA AGACTTGGTT 
CGTCAGATGA ATGAGTATAC TGGAGAAGAA CCCAGTCTGG CTGAGCAGAG ACTCCAGCGG 
CACGCAGAGA GGAAACTGGC CATGACTAAC ATGACCTACG AAGTTATGCA GCAGGGTCAG 
GACCTGCATC AGTACATCAT GGAAGTCCAA GCTTCAGGTA TTGAGTTGAC CGGAGAGAAG 
GAGGTGGATC TGGCAACACA AGTTCAGGAG CTGCTGGAGT TCCTTCATGA GAAACAGCAC 
GAGTTGGACA TGAGCGCCGA ACACACACAG AAGCGCCTGG AGCAGTGTGT GCAGCTCCGC 
CACCTGCAGG CTGAGGTTAA ACAGGTTCTG GGGTGGATTC GTAATGGTGA ATCTATGTTG 
GATGCCAGTA TGGTGAATGC AAGTTCACTG TCAGAGGCCG AACAACTACA GAGAGAACAT 
GAACAGTTCC AGCTGGCCAT AGAGTCTTTG TTCCACGCTA ACTCTCTCCA GAAAACCCAT 
CAGAGTGCAC TACAGGTCCA GCAGAAGGCA GAAATCATGT TACAGGCTGG ACACTTTGAC 
CCCGAGGCCA TCCGCAGCTG TGCAGAGAAG GTGGCCGTGC ACTGGCAGCA GCTCATGCTC 
AAGATGGAGG ACAGACTCAA ACTGGTTAAT GCCTCGGTGG CCTTCTACAA GACGTCAGAA 
CAGGTGTGTA GTGTCCTGGA GAGTCTGGAG CAGGAGTACC GCAGAGATGA AGACTGGTGT 
GGAGCTCATG ATAAACTAGG ATCTTCATCA GAGACTGATC ACGTTCTGCC TCTCATCAGC 
AAACACCTGG AGCAGAAAGA AGCTTTTCTC AAGGCATGCA CTCTGGCCAG GCGCAATGCT 
GAGGTTTTTC TGAAATACAT CCACCGGAAC AATGTCAGCT CTCAGGGATC ATCTAGCAAC 
ACTCGTGGCC CTGAGCAACA GGTCAAAGCC ATCCTGAATG AACTGCTGCA GAGGGAAAAC 
AGAGTTTTGC ATTTCTGGAC CATGAAGAAG CGGCGTTTGG ATCAGTGTCA GCAGTATGTG 
GTGTTTGAAC GCAATGCCAA ACAGGCATTA GAGTGGATTG AGGAGGCAGG AGAGGTCTAT 
CTGGCCACTC ACACCTGCAT CGGAGACTCT ATGGAGAAAA CTCAGGGACT CCTCAAAGAA 
TACGAAGAAT TCTGCATCTC CGCAAAGCAA ACTAAGGAGA AAGTGAAGAT GCTGATCCAG 
CTGGCGGATA ACTTTGTTGA GAAAGGACAC ATGCATGTCA CCGACTTGAA GAAGTGTCTG 
ACGGCAGTGG ACAAGCGCTA CAGGGACTTC TCCATGCGCA TGGGCAAATA CCGCTGTGAC 
CTGGAAAATG TGCTGGGCAT CAGCTCTGAG GATAATAAGG ATCTGGAGCT GGATATCATC 
CCAGCCAGTC TGACTGGCCC AGAGGTTAAA CTCCGTGACC CCAACCACGA GGTGAATGAA 
GAAAAGAGGA AATCAGCCCG GAAGAAAGAG TTTATTATAG CAGAGCTGTT GCAGACAGAA 
AAGGCTTATG TGAGGGACTT ACATGAATGC TTAGAGACAT ATTTGTGGGA GATGACCAAT 
GGTGTGGAGG AAATTCCTCC AGGAATCGCC AACAAAGAGC ACATCATCTT TGGCAACATT 
CAGGAGATTT ACGACTTCCA CAATAATATA TTCCTGAAGG AGCTGATCAA TTATGAGCAA 
CTGCCTGAGG ATGTGGGACA TTGCTTTGTG ACATGGGCCG ATAAGTTTAA CATTTATGTG 
ACATACTGCA AAAACAAACC AGATTCCAGT CAGCTCATCC TAGACCATGC TGGGAGTTTT 
TTTGATGAGA TTCAGAAGAG ACACAGTCTG ACCAACTCTA TATCATCATA CCTCATCAAA 
CCTGTACAGA GGATCACCAA ATATCAGCTG CTGCTCAAGG AGCTGCTGTC CTGCTGTGAA 
GAAGGCAAAG GTGAAATTAA AGATGGCCTG GAGGTCATGC TGAGTGTGCC AAAGAGAGCC 
AATGACGCCA TGCACGTCAG CATGCTGGAG GGTTTTGATG CAAATTTGGA TGTTCAGGGT 
GAACTCATCC TCCAAGACAC CTTCCAGGTG TGGGACCCCA AATCACTAAT CCGGAAGGGG 
CGAGACCGCC ACCTCTTTCT CTTCGAGATC TCCCTGGTCT TCAGTAAAGA GATCAAAGAC 
TCAGCAGGGC GATCCAAATA CATCTTTAAA AACAAACTAC TGACTTCTGA ATTGGGGGTG 
ACAGAGCATG TAGAGGGGGA CCCGTGCAAA TTTGCTCTGT GGGTGGGACG CACACCATCG 
TCTGACAACA AGACTGTCCT CAAGGCATCT AGTATTGAGG TGAAGCAAGA ATGGATTAAA 
AACATCCGAG AGGTCATTCA GGAGAGAATA ATTCACCTGA AGGGGGCGCT AAAGGAACCT 
ATCCACATTC CCAAAACTCC AGCCAAACCT CGCAATAACA GCAAGAGGGA CGCTGGTGAG 
GATGCTGACA GCCAGGGAGA CGGCAGCAGT CAGCCGGACA CCATCTCCAT AGCATCCAGA 
ACCTCTCAGA ACACTGTAGA CAGTGACAAG CTCTCTGGAG GGTGTGAGCT GACGGTGGTC 
CTGCAGGACT TTACAGCAAG CAGCTCATCA GAGCTCAGCA TCCAGACGGG TCAGACAGTG 
GAGCTGCTGG AGCGGCCCAG TGACCGTCCA GGATGGTGTC TGGTACGGAC CACCGAAAAG 
ACGCCTCCTC AAGAGGGACT GGTGCCCAGC AGTGCTCTGT GTATCTCGCA CTCACGCAGC 
AGTGTGGAGA TGGACTGCTT TTTCCCAGCA GGCAAAGAAA ACTATACAGT TAGTGGCCCA 
GAAGGAAAGA CAGAGTCAGT GGCCACACTG CAGCCACAGC CATCCCTGAA CTCTCTGCAG 
AGTAGCTCTC CTGGACCCAA GCGCTCTGGG AATACACTGA AGAAATGGCT GACCAGTCCT 
GTCCGACGTC TAAGTCATGG CAGTAACATC AAGAAGATCC CAAGTAAACA GAAACAGAAA 
CGTGATGGTC GCAAGAGTAT TGATATGGGG CCGCCCGAAC TACAGGATGA CACCTTGGAA 
GAGCGAGTGC AAAAAGAAGA GGGCACACTC TCCAAGTCTT CGTCTGGGAT GCACAGTGGA 
GGAGAGGAAG AGCCTGAGGA CGAGTCTCAC ACTCCTCTAC CTCCACCAAT GGAGATCATT 
AAAGACCCGT CGGCTCAGGA AGAGAAGTCG TCTGTGCTTC TGGCTTCCCG GCAGAGCTCG 
GCTGATGTCC CCAGTGCTGC TGAGCTTGTC AGTGCTATAG AAAAACTGGT CAAAACTAAA 
ATGACACTGG AGCCAGGCTC ATACCCGGGA TTCAGCTCAG TCAATCCAGC AGAGCAGAGC 
CCGATTCCAC CCAGAAACCC AGAACTAGAG CAGAAAGCCA AAGCGCTACG AGGACGAATG 
TTTGTAGTCA AGGAATTAAT CCAAACGGAA AAAGACTATG TTAAAGATCT GGGAATTGTG 
GTTGAGGGGT TTATGAAGAG AATCGAGGAG AAAGGAGTTC CTGATAACAT GAAAGGAAAA 
GATAAGATCG TCTTCGGAAA CATTCACCAA ATCTATGACT GGCACAAAGA TTTTTTTGTT 
GGAGAACTGG AGAAATGCCT GGAAGATCAC GAAAAACTGC CTGAACTGTT CTGTAAACAT 
GAGAGACGGT TACACATGTA TGTAGTTTAC TGTCAGAACA AACCCAAATC CGAGTTCATC 
GTTGCAGAAT ATGATTCATA CTTTGAGGGG ATTCAGCAAG ACATTAACTC CAGACTGAGC 
ATCAGCGATT TTCTCATTAA ACCCATTCAG AGAATTACTA AATACCAGCT TTTATTAAAG 
GATGTTTTGA AGTACAGTAC AAAAGCAGGA CTGGATTGTC AGGATATAGA GAATGCAGTT 
GACCTGATGT CCCAGGTTCC CAAGCTTTGC AATGACATGA TGAATCTGGG AAGACTGCAG 
GGATATGAGG GGAAGTTGAC GAGTCAAGGA AAACTCTTGC AGCAGGAGAC GTTCTTTGTG 
ACCGAGCAGG ACTCTGGCGT TCTGTCCCGC AGTAAAGAGC GCAGGGTTTT CCTGTTCGAA 
CAAATTGTCA TCTTCAGCGA GCTGTTACGT AAAGGCTCTT CCACTCCAGG GTACCAGTTC 
AAGAAGAGCA TAAAGGTGAG CTTCCTTGAC TTGGAAGAGC ATGTTGAAAA TGATCCATGC 
AAGTTTGTGC TGTCGTGTCG TGGGTCGGCA GAACGTTTTA CCCTTCAAGC AGCAAACACG 
GACATTAAAC AAGTCTGGGT TCAGCATATC ACCCAACTAC TAGATTTACA AAGCAACTTC 
TTGTCAGCTC TGCAGTCTCC TATTGAGTAT CAGAAGGAGC GTAGTGGCTC TCAATCTCTG 
ACCCGGAACT CTTCTGGGCG GATGGGTCAG TCCAACAGCA GGCCCTGCTC CACGGTTTCA 
CTCGGAGCAG AGAAACCCTC TCTGTCTGGA CGGAGCTCCA CCCCGCTAAA GCTATCTACC 
TCCAATGGTG GTCCATGCTC TGAAAGATAC CGCAGGCAGT TTGAAGGCTT GGGCTGCAAC 
GGTACATCGT CCTCCTTGAT GGTGACTCAA GATTATACTG CACTGAAAGA GAATGAGATC 
TGTGTCACAC AGGGTGAGGC CGTGCAGGTA CTGGCCACTA ACCAGCAGAA CATGTACCTT 
GTGTACCGAC CCGCAAATAG CCAATCACCA CCTGCAGAGG GCTGGGTGCC GGGACACATC 
CTAGGCCCCG TCACAAAACC TCTCATAGAC ACTACTGCAG ACGCAAGCAT CAAGAAATCT 
CTTTCGTGGA ACACACTTCG TGCTCGTAAG CGTGCAGAAA AGGACAGCAC GCCCCTCAAA 
AGTGACTTTA AAGTTGAGAA TGGACTGCGT AAGCCAAAGG AAATTCTCAG CAGCAAAGTC 
AAAGTGAAAG AGTCCCGCAG TTCTGATGAG TCTGAATGTG AGGATGAACT AGACCCCAAA 
ACTAGTATGG AGTTACTGAA CCCTAATTTC ATCCAAGAGG TCGCTCCAGA GTTTCTGACG 
CCTCTAACAG ACGTGACGTG TGCTCTGGGA GAGACGGTGG TTCTGTGCTG TAAAGTGTGT 
GCTCGACCCA AACCCACAAT CACCTTGAAA GGACCTGATC AAAGTCTGCT GGCCAACAAT 
AACCGCTTCA CTATTAATAT CAGAGAAACC GGTGATATAG TGTTGAAAAT CTGCAATCTA 
ATGCCGCAGG ACACTGGCAT CTACACGTGT GTTGCTGTGA ATGACCATGG TACAGCCTCC 
TCATCTGCAT CCATTAAAGT ACAAGGTATC CCAGCAGCCC CTGCTCGACC GGTGGCACAG 
GAGGCAAGCA GCACTGCAGT GATTGTCCAC TGGCTTCCTC CAGCCAGCTC AGCAAATTCT 
GCCATCTCCA GCTACACTGT GGAATATAGG CAGGAAGATT CTCTGGTATG GCAGCAGTTG 
GTGGTCTCCA CGGCAGATGT GTTTGTGAAG ATTGACAATC TGATTCCTGG TGGGCACTAC 
CAGTTCAGAG TCAGTGCCAG TAATCCCTGG GGCACCAGCC CGCCTAGTGA ACCCTCAAAC 
ATGGTGACAT TATCCAGCAC AGCATCAACA TATGATGGCG CTGGAATCCA GTGGAAAGAC 
AACTTTGAAT CTGCTTTTAC AGAATTATGT GAAATTGGGC GGGGTCGTTT CTCTGTGGTG 
AGGAAGTGTC TCAGTAAAGC CACTAAGCAG GAGGTTGCTG TGAAGTTTGT GAATAAGAAG 
ATGCACAAGA AAGAACAGGT GGCCCATGAA GCTGATATTC TACGCCATGT CCAGCACCCC 
CAACTGGTGT CACTGATTGA TACATACGAG TCGCCTACAG CCTACATGCT GATTCTTGAA 
CTTGTTGAGG ATGGACGTCT TCTTGACTAT CTGGTAGCTC ATGATGAGCT GATGGAGGAG 
AAAGTGGCAT TCTTTATTAA AGATACACTG GAAGCTCTGC AGCACTTGCA CACCTGCAGA 
GTGGCTCATC TAGACCTCAA GCCTGAAAAC TTGTTGGTGG ACCTCCACGT TCCGGTACCC 
TGCGTGAAGC TCTCAGATCT AGGTGATGCT GTACAGGTTT CAAGTCACCG CTACGTACAT 
CTCCTCCTGG GTAACCCCGA GTTCGCTGCT CCAGAGCTCA TCCAGGGCAC TCCAGTGTCC 
CTCTCTACAG ATGTGTGGAG TGTAGGGGTG CTGGCCTACG TCATGCTTAG TGGAGTCTCC 
CCATTCCTGG ATGAGAGTCT GGAGGAGACC TGCGTCAACA TCTGCAGGCT GGACTTCTGC 
TTTCCTCAAG AGTACTTCTC CGATATAAGC CAGGCAGCCA AAGACTTCAT TGTGTCCACG 
CTTAACCAGG ATCCCAGGAA GAGGCCAAGC TCGGCCTCGT GTCTTCAGCA CCCATGGGTC 
AGTGCTCATA GTGGAGATTA CTCCAAAACA CCTCTGGATA CTGTCAGACT CGCTGCATTT 
ATAGACAGAC GCAAACAGCT ACATGATGTC AGGCCTGTCA CAAACATTAA AGGAATGGTC 
AGCAGCAGTA TGGGACACAC CTTATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kalrna ( XM_009304450.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302725.2 Danio rerio kalirin, RhoGEF kinase a (kalrna), transcript variant X2, mRNA.

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CloneID	ODa60288
Clone ID Related Accession (Same CDS sequence)	XM_009304450.2 , XM_009304450.3
Accession Version	XM_009304450.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kalirin isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304450.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTG CTGAAGCGCC GGAGGACGGA CCCAATGATT CGGACACGGA TGCATTCTTC 
AAAACAGGGT CTTTCAGAAG TGAAGGAATT AAAGCCTCTG ATGTCCTTCC TGTGCTGAGA 
GAAAAAATAG CATTTGTGTC AGGCGGTCGT GATAAGCGAG GTGGCCCTAT ACTGACCTTT 
CCTGCAAGGA GTAACCATGA CCGAATAAAA CAAGAGGACC TGAGACGGCT CGTAACATAC 
CTGTCCACTG TGCCCAGTGA GGACGTTTGT AAGAGAGGCT TCACTGTGAT TATTGATATG 
CGTGGCTCTA AATGGGAGCT AATAAAGCCA CTGCTGAAGA CGCTACAGGA AGCATTTCCA 
GCTGAGATCT GCGTCGCTCT CATCATCAAA CCAGACAACT TCTGGCAGAA GCAGAAGACT 
AACTTTGGTA GCGCCAAGTT CACCTTTGAG ACTAGCATGG TATCAGTGGA GGGCTTGACC 
AAACTAGTGG ACCCATCCCA GCTAACGGAC GACTTTGAAG GTTCTCTGGA ATACAATCAT 
GAGGAATGGC TTGAGCTCCG GGTGGCTCTG GAGGAGTTCC TGGCCAGCGC CGTTCACCTC 
CTCTCTCGCC TGGAAGATCT ACAGGAGACT CTGGCTAAGA AGGAGTTCCC GGTGGATGTG 
GAAGCCTCTC GTAGACTCAT CGATGAGCAC ACACAGCTGA AGAAGAAGGT GATGAAGGCT 
CCGGTGGAAG AGCTGGACCA CGAAGGCCAG AGACTCTTGC AGTGCATCCG CTCCAGCGAC 
GGCTTCTCAG GGAGGAACTG CATTTCTGGA AGTGCCGACT TCCAGAGCGT GGTTCCGAAG 
ATCGCCAGTC TGCTGGATAA ACTCCACAGC ACTCGACAGC ACTTGCACCA GATGTGGCAC 
CTACGAAAAC TCAAGCTGGA TCAGTGCTTT CAGCTCCGAC TGTTTGAACA GGACGCAGAG 
AAGATGTTTG ACTGGATCAG TCACAATAAG GAATTGTTTC TTCAGAGCCA CACGGAGATC 
GGTGTGAGCT ACCAGCATGC TGTGGAGCTA CAGACGCAAC ACAACCACTT TGCCATGAAC 
TCAATGAATG CCTATGTGAA CATAAACCGC ATCATGTCGG TGGCGTCGCG GCTGTCGGAG 
GCGGGCCACT ACGCCGCTCA GCAGATCAAA CAGATCAGCA CTCAGCTGGA TCAGGAGTGG 
AAGAGCTTCG CGGCTGCTCT CGATGAACGC AGCACCATCC TTGCCATGTC TGCCGTCTTC 
CATCAGAAAT CTGAACAGTT TTTGTCCGGT GTGGAAACAT GGTGTAAGAT GTGCAGTGAA 
GGCGGCTTGC CATCAGAGAT GCAGGATCTG GAATCGGCCA TTCATCACCA TCAGACGCTC 
TATGAACAAG TCACACAAGC ATACACCGAG GTGAGTCAGG ACGGTAAGGC TTTGCTGGAC 
GTTCTTCAGC GGCCGTTGAG TCCTGGGAAC GCAGAGTCAC TGACTGCGAC AGCGAACTAC 
TCTAAAGCCG TGCACTGTAT ACTGGACGTG GTCCACGAGG TCCTGCATCA TCAGAGACGT 
CTGGAGAACA TCTGGCAGCA CCGTAAAGTC CGTCTGCATC AGAGACTGCA GCTCTGTGTC 
TTCCAGCAGG ACGTCCAACA GGTGATTGAC TGGATAGAGA ATCACGGGGA GGCTTTCCTC 
AGCAAACACA CTGGTGTGGG CAAATCCCTG CACAGAGCCA GAGCCCTCCA GAAACGCCAT 
GATGACTTTG AGGAGGTGGC ACAGAATACA TACACTAATG CAGATAAGCT GCTGGAAGCT 
GCTGAGCAGT TGGCTCAGAC GGGCGAGTGT GACCCTGAGG AGATCTACAA GGCAGCGCGA 
CACCTGGAAG TCAGGATCCA GGACTTTGTT AGGCGAGTGG AGCAAAGAAA ACTACTGCTG 
GACATGTCTG TCTCTTTCCA CACGCACACT AAAGAGTTGT GGACGTGGAT GGAGGACCTG 
CAGAAAGAGC TGCTGGAGGA AGTTTGTTCT GACTCAGTCG ATTCAGTTCA GGAGCTGATA 
AAACAGTTTC AGCAGCAGCA GACGGCCACG CTGGAGGCAA CACTCAATGT CATTAAAGAG 
GGAGAAGATC TTATGCAACA ACTCCGAGAT TCAGCAATGA GCAGTAATAA GATGCCCCAC 
ACCAGCTCCA TCGCTCACAT TGAGGGCGTC CTGCAGCAGC TAGAGGAGGC GCAGGGCCAC 
ATGGAGGAAC TCTTCCATGA GAGAAAGATC AAACTCGACA TCTTCCTGCA ACTGCGCATC 
TTTGAGCAGT ACACCATTGA GGTAACGGCA GAGTTAGACG CGTGGAATGA AGACTTGGTT 
CGTCAGATGA ATGAGTATAC TGGAGAAGAA CCCAGTCTGG CTGAGCAGAG ACTCCAGCGG 
CACGCAGAGA GGAAACTGGC CATGACTAAC ATGACCTACG AAGTTATGCA GCAGGGTCAG 
GACCTGCATC AGTACATCAT GGAAGTCCAA GCTTCAGGTA TTGAGTTGAC CGGAGAGAAG 
GAGGTGGATC TGGCAACACA AGTTCAGGAG CTGCTGGAGT TCCTTCATGA GAAACAGCAC 
GAGTTGGACA TGAGCGCCGA ACACACACAG AAGCGCCTGG AGCAGTGTGT GCAGCTCCGC 
CACCTGCAGG CTGAGGTTAA ACAGGTTCTG GGGTGGATTC GTAATGGTGA ATCTATGTTG 
GATGCCAGTA TGGTGAATGC AAGTTCACTG TCAGAGGCCG AACAACTACA GAGAGAACAT 
GAACAGTTCC AGCTGGCCAT AGAGTCTTTG TTCCACGCTA ACTCTCTCCA GAAAACCCAT 
CAGAGTGCAC TACAGGTCCA GCAGAAGGCA GAAATCATGT TACAGGCTGG ACACTTTGAC 
CCCGAGGCCA TCCGCAGCTG TGCAGAGAAG GTGGCCGTGC ACTGGCAGCA GCTCATGCTC 
AAGATGGAGG ACAGACTCAA ACTGGTTAAT GCCTCGGTGG CCTTCTACAA GACGTCAGAA 
CAGGTGTGTA GTGTCCTGGA GAGTCTGGAG CAGGAGTACC GCAGAGATGA AGACTGGTGT 
GGAGCTCATG ATAAACTAGG ATCTTCATCA GAGACTGATC ACGTTCTGCC TCTCATCAGC 
AAACACCTGG AGCAGAAAGA AGCTTTTCTC AAGGCATGCA CTCTGGCCAG GCGCAATGCT 
GAGGTTTTTC TGAAATACAT CCACCGGAAC AATGTCAGCT CTCAGGGATC ATCTAGCAAC 
ACTCGTGGCC CTGAGCAACA GGTCAAAGCC ATCCTGAATG AACTGCTGCA GAGGGAAAAC 
AGAGTTTTGC ATTTCTGGAC CATGAAGAAG CGGCGTTTGG ATCAGTGTCA GCAGTATGTG 
GTGTTTGAAC GCAATGCCAA ACAGGCATTA GAGTGGATTG AGGAGGCAGG AGAGGTCTAT 
CTGGCCACTC ACACCTGCAT CGGAGACTCT ATGGAGAAAA CTCAGGGACT CCTCAAAGAA 
TACGAAGAAT TCTGCATCTC CGCAAAGCAA ACTAAGGAGA AAGTGAAGAT GCTGATCCAG 
CTGGCGGATA ACTTTGTTGA GAAAGGACAC ATGCATGTCA CCGACTTGAA GAAGTGTCTG 
ACGGCAGTGG ACAAGCGCTA CAGGGACTTC TCCATGCGCA TGGGCAAATA CCGCTGTGAC 
CTGGAAAATG TGCTGGGCAT CAGCTCTGAG GATAATAAGG ATCTGGAGCT GGATATCATC 
CCAGCCAGTC TGACTGGCCC AGAGGTTAAA CTCCGTGACC CCAACCACGA GGTGAATGAA 
GAAAAGAGGA AATCAGCCCG GAAGAAAGAG TTTATTATAG CAGAGCTGTT GCAGACAGAA 
AAGGCTTATG TGAGGGACTT ACATGAATGC TTAGAGACAT ATTTGTGGGA GATGACCAAT 
GGTGTGGAGG AAATTCCTCC AGGAATCGCC AACAAAGAGC ACATCATCTT TGGCAACATT 
CAGGAGATTT ACGACTTCCA CAATAATATA TTCCTGAAGG AGCTGATCAA TTATGAGCAA 
CTGCCTGAGG ATGTGGGACA TTGCTTTGTG ACATGGGCCG ATAAGTTTAA CATTTATGTG 
ACATACTGCA AAAACAAACC AGATTCCAGT CAGCTCATCC TAGACCATGC TGGGAGTTTT 
TTTGATGAGA TTCAGAAGAG ACACAGTCTG ACCAACTCTA TATCATCATA CCTCATCAAA 
CCTGTACAGA GGATCACCAA ATATCAGCTG CTGCTCAAGG AGCTGCTGTC CTGCTGTGAA 
GAAGGCAAAG GTGAAATTAA AGATGGCCTG GAGGTCATGC TGAGTGTGCC AAAGAGAGCC 
AATGACGCCA TGCACGTCAG CATGCTGGAG GGTTTTGATG CAAATTTGGA TGTTCAGGGT 
GAACTCATCC TCCAAGACAC CTTCCAGGTG TGGGACCCCA AATCACTAAT CCGGAAGGGG 
CGAGACCGCC ACCTCTTTCT CTTCGAGATC TCCCTGGTCT TCAGTAAAGA GATCAAAGAC 
TCAGCAGGGC GATCCAAATA CATCTTTAAA AACAAACTAC TGACTTCTGA ATTGGGGGTG 
ACAGAGCATG TAGAGGGGGA CCCGTGCAAA TTTGCTCTGT GGGTGGGACG CACACCATCG 
TCTGACAACA AGACTGTCCT CAAGGCATCT AGTATTGAGG TGAAGCAAGA ATGGATTAAA 
AACATCCGAG AGGTCATTCA GGAGAGAATA ATTCACCTGA AGGGGGCGCT AAAGGAACCT 
ATCCACATTC CCAAAACTCC AGCCAAACCT CGCAATAACA GCAAGAGGGA CGCTGGTGAG 
GATGCTGACA GCCAGGGAGA CGGCAGCAGT CAGCCGGACA CCATCTCCAT AGCATCCAGA 
ACCTCTCAGA ACACTGTAGA CAGTGACAAG CTCTCTGGAG GGTGTGAGCT GACGGTGGTC 
CTGCAGGACT TTACAGCAAG CAGCTCATCA GAGCTCAGCA TCCAGACGGG TCAGACAGTG 
GAGCTGCTGG AGCGGCCCAG TGACCGTCCA GGATGGTGTC TGGTACGGAC CACCGAAAAG 
ACGCCTCCTC AAGAGGGACT GGTGCCCAGC AGTGCTCTGT GTATCTCGCA CTCACGCAGC 
AGTGTGGAGA TGGACTGCTT TTTCCCAGCA GGCAAAGAAA ACTATACAGT TAGTGGCCCA 
GAAGGAAAGA CAGAGTCAGT GGCCACACTG CAGCCACAGC CATCCCTGAA CTCTCTGCAG 
AGTAGCTCTC CTGGACCCAA GCGCTCTGGG AATACACTGA AGAAATGGCT GACCAGTCCT 
GTCCGACGTC TAAGTCATGG CAGTAACATC AAGAAGATCC CAAGTAAACA GAAACAGAAA 
CGTGATGGTC GCAAGAGTAT TGATATGGGG CCGCCCGAAC TACAGGATGA CACCTTGGAA 
GAGCGAGTGC AAAAAGAAGA GGGCACACTC TCCAAGTCTT CGTCTGGGAT GCACAGTGGA 
GGAGAGGAAG AGCCTGAGGA CGAGTCTCAC ACTCCTCTAC CTCCACCAAT GGAGATCATT 
AAAGACCCGT CGGCTCAGGA AGAGAAGTCG TCTGTGCTTC TGGCTTCCCG GCAGAGCTCG 
GCTGATGTCC CCAGTGCTGC TGAGCTTGTC AGTGCTATAG AAAAACTGGT CAAAACTAAA 
ATGACACTGG AGCCAGGCTC ATACCCGGGA TTCAGCTCAG TCAATCCAGC AGAGCAGAGC 
CCGATTCCAC CCAGAAACCC AGAACTAGAG CAGAAAGCCA AAGCGCTACG AGGACGAATG 
TTTGTAGTCA AGGAATTAAT CCAAACGGAA AAAGACTATG TTAAAGATCT GGGAATTGTG 
GTTGAGGGGT TTATGAAGAG AATCGAGGAG AAAGGAGTTC CTGATAACAT GAAAGGAAAA 
GATAAGATCG TCTTCGGAAA CATTCACCAA ATCTATGACT GGCACAAAGA TTTTTTTGTT 
GGAGAACTGG AGAAATGCCT GGAAGATCAC GAAAAACTGC CTGAACTGTT CTGTAAACAT 
GAGAGACGGT TACACATGTA TGTAGTTTAC TGTCAGAACA AACCCAAATC CGAGTTCATC 
GTTGCAGAAT ATGATTCATA CTTTGAGGGG ATTCAGCAAG ACATTAACTC CAGACTGAGC 
ATCAGCGATT TTCTCATTAA ACCCATTCAG AGAATTACTA AATACCAGCT TTTATTAAAG 
GATGTTTTGA AGTACAGTAC AAAAGCAGGA CTGGATTGTC AGGATATAGA GAATGCAGTT 
GACCTGATGT CCCAGGTTCC CAAGCTTTGC AATGACATGA TGAATCTGGG AAGACTGCAG 
GGATATGAGG GGAAGTTGAC GAGTCAAGGA AAACTCTTGC AGCAGGAGAC GTTCTTTGTG 
ACCGAGCAGG ACTCTGGCGT TCTGTCCCGC AGTAAAGAGC GCAGGGTTTT CCTGTTCGAA 
CAAATTGTCA TCTTCAGCGA GCTGTTACGT AAAGGCTCTT CCACTCCAGG GTACCAGTTC 
AAGAAGAGCA TAAAGGTGAG CTTCCTTGAC TTGGAAGAGC ATGTTGAAAA TGATCCATGC 
AAGTTTGTGC TGTCGTGTCG TGGGTCGGCA GAACGTTTTA CCCTTCAAGC AGCAAACACG 
GACATTAAAC AAGTCTGGGT TCAGCATATC ACCCAACTAC TAGATTTACA AAGCAACTTC 
TTGTCAGCTC TGCAGTCTCC TATTGAGTAT CAGAAGGAGC GTAGTGGCTC TCAATCTCTG 
ACCCGGAACT CTTCTGGGCG GATGGGTCAG TCCAACAGCA GGCCCTGCTC CACGGTTTCA 
CTCGGAGCAG AGAAACCCTC TCTGTCTGGA CGGAGCTCCA CCCCGCTAAA GCTATCTACC 
TCCAATGGTG GTCCATGCTC TGAAAGATAC CGCAGGTTTG AAGGCTTGGG CTGCAACGGT 
ACATCGTCCT CCTTGATGGT GACTCAAGAT TATACTGCAC TGAAAGAGAA TGAGATCTGT 
GTCACACAGG GTGAGGCCGT GCAGGTACTG GCCACTAACC AGCAGAACAT GTACCTTGTG 
TACCGACCCG CAAATAGCCA ATCACCACCT GCAGAGGGCT GGGTGCCGGG ACACATCCTA 
GGCCCCGTCA CAAAACCTCT CATAGACACT ACTGCAGACG CAAGCATCAA GAAATCTCTT 
TCGTGGAACA CACTTCGTGC TCGTAAGCGT GCAGAAAAGG ACAGCACGCC CCTCAAAAGT 
GACTTTAAAG TTGAGAATGG ACTGCGTAAG CCAAAGGAAA TTCTCAGCAG CAAAGTCAAA 
GTGAAAGAGT CCCGCAGTTC TGATGAGTCT GAATGTGAGG ATGAACTAGA CCCCAAAACT 
AGTATGGAGT TACTGAACCC TAATTTCATC CAAGAGGTCG CTCCAGAGTT TCTGACGCCT 
CTAACAGACG TGACGTGTGC TCTGGGAGAG ACGGTGGTTC TGTGCTGTAA AGTGTGTGCT 
CGACCCAAAC CCACAATCAC CTTGAAAGGA CCTGATCAAA GTCTGCTGGC CAACAATAAC 
CGCTTCACTA TTAATATCAG AGAAACCGGT GATATAGTGT TGAAAATCTG CAATCTAATG 
CCGCAGGACA CTGGCATCTA CACGTGTGTT GCTGTGAATG ACCATGGTAC AGCCTCCTCA 
TCTGCATCCA TTAAAGTACA AGGTATCCCA GCAGCCCCTG CTCGACCGGT GGCACAGGAG 
GCAAGCAGCA CTGCAGTGAT TGTCCACTGG CTTCCTCCAG CCAGCTCAGC AAATTCTGCC 
ATCTCCAGCT ACACTGTGGA ATATAGGCAG GAAGATTCTC TGGTATGGCA GCAGTTGGTG 
GTCTCCACGG CAGATGTGTT TGTGAAGATT GACAATCTGA TTCCTGGTGG GCACTACCAG 
TTCAGAGTCA GTGCCAGTAA TCCCTGGGGC ACCAGCCCGC CTAGTGAACC CTCAAACATG 
GTGACATTAT CCAGCACAGC ATCAACATAT GATGGCGCTG GAATCCAGTG GAAAGACAAC 
TTTGAATCTG CTTTTACAGA ATTATGTGAA ATTGGGCGGG GTCGTTTCTC TGTGGTGAGG 
AAGTGTCTCA GTAAAGCCAC TAAGCAGGAG GTTGCTGTGA AGTTTGTGAA TAAGAAGATG 
CACAAGAAAG AACAGGTGGC CCATGAAGCT GATATTCTAC GCCATGTCCA GCACCCCCAA 
CTGGTGTCAC TGATTGATAC ATACGAGTCG CCTACAGCCT ACATGCTGAT TCTTGAACTT 
GTTGAGGATG GACGTCTTCT TGACTATCTG GTAGCTCATG ATGAGCTGAT GGAGGAGAAA 
GTGGCATTCT TTATTAAAGA TACACTGGAA GCTCTGCAGC ACTTGCACAC CTGCAGAGTG 
GCTCATCTAG ACCTCAAGCC TGAAAACTTG TTGGTGGACC TCCACGTTCC GGTACCCTGC 
GTGAAGCTCT CAGATCTAGG TGATGCTGTA CAGGTTTCAA GTCACCGCTA CGTACATCTC 
CTCCTGGGTA ACCCCGAGTT CGCTGCTCCA GAGCTCATCC AGGGCACTCC AGTGTCCCTC 
TCTACAGATG TGTGGAGTGT AGGGGTGCTG GCCTACGTCA TGCTTAGTGG AGTCTCCCCA 
TTCCTGGATG AGAGTCTGGA GGAGACCTGC GTCAACATCT GCAGGCTGGA CTTCTGCTTT 
CCTCAAGAGT ACTTCTCCGA TATAAGCCAG GCAGCCAAAG ACTTCATTGT GTCCACGCTT 
AACCAGGATC CCAGGAAGAG GCCAAGCTCG GCCTCGTGTC TTCAGCACCC ATGGGTCAGT 
GCTCATAGTG GAGATTACTC CAAAACACCT CTGGATACTG TCAGACTCGC TGCATTTATA 
GACAGACGCA AACAGCTACA TGATGTCAGG CCTGTCACAA ACATTAAAGG AATGGTCAGC 
AGCAGTATGG GACACACCTT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302725.2
CDS	237..9140
Translation	MNSAEAPEDGPNDSDTDAFFKTGSFRSEGIKASDVLPVLREKIAFVSGGRDKRGGPILTFPARSNHDRIKQEDLRRLVTYLSTVPSEDVCKRGFTVIIDMRGSKWELIKPLLKTLQEAFPAEICVALIIKPDNFWQKQKTNFGSAKFTFETSMVSVEGLTKLVDPSQLTDDFEGSLEYNHEEWLELRVALEEFLASAVHLLSRLEDLQETLAKKEFPVDVEASRRLIDEHTQLKKKVMKAPVEELDHEGQRLLQCIRSSDGFSGRNCISGSADFQSVVPKIASLLDKLHSTRQHLHQMWHLRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHAVELQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYAAQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKSEQFLSGVETWCKMCSEGGLPSEMQDLESAIHHHQTLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNAESLTATANYSKAVHCILDVVHEVLHHQRRLENIWQHRKVRLHQRLQLCVFQQDVQQVIDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKELLEEVCSDSVDSVQELIKQFQQQQTATLEATLNVIKEGEDLMQQLRDSAMSSNKMPHTSSIAHIEGVLQQLEEAQGHMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLVRQMNEYTGEEPSLAEQRLQRHAERKLAMTNMTYEVMQQGQDLHQYIMEVQASGIELTGEKEVDLATQVQELLEFLHEKQHELDMSAEHTQKRLEQCVQLRHLQAEVKQVLGWIRNGESMLDASMVNASSLSEAEQLQREHEQFQLAIESLFHANSLQKTHQSALQVQQKAEIMLQAGHFDPEAIRSCAEKVAVHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGAHDKLGSSSETDHVLPLISKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSSQGSSSNTRGPEQQVKAILNELLQRENRVLHFWTMKKRRLDQCQQYVVFERNAKQALEWIEEAGEVYLATHTCIGDSMEKTQGLLKEYEEFCISAKQTKEKVKMLIQLADNFVEKGHMHVTDLKKCLTAVDKRYRDFSMRMGKYRCDLENVLGISSEDNKDLELDIIPASLTGPEVKLRDPNHEVNEEKRKSARKKEFIIAELLQTEKAYVRDLHECLETYLWEMTNGVEEIPPGIANKEHIIFGNIQEIYDFHNNIFLKELINYEQLPEDVGHCFVTWADKFNIYVTYCKNKPDSSQLILDHAGSFFDEIQKRHSLTNSISSYLIKPVQRITKYQLLLKELLSCCEEGKGEIKDGLEVMLSVPKRANDAMHVSMLEGFDANLDVQGELILQDTFQVWDPKSLIRKGRDRHLFLFEISLVFSKEIKDSAGRSKYIFKNKLLTSELGVTEHVEGDPCKFALWVGRTPSSDNKTVLKASSIEVKQEWIKNIREVIQERIIHLKGALKEPIHIPKTPAKPRNNSKRDAGEDADSQGDGSSQPDTISIASRTSQNTVDSDKLSGGCELTVVLQDFTASSSSELSIQTGQTVELLERPSDRPGWCLVRTTEKTPPQEGLVPSSALCISHSRSSVEMDCFFPAGKENYTVSGPEGKTESVATLQPQPSLNSLQSSSPGPKRSGNTLKKWLTSPVRRLSHGSNIKKIPSKQKQKRDGRKSIDMGPPELQDDTLEERVQKEEGTLSKSSSGMHSGGEEEPEDESHTPLPPPMEIIKDPSAQEEKSSVLLASRQSSADVPSAAELVSAIEKLVKTKMTLEPGSYPGFSSVNPAEQSPIPPRNPELEQKAKALRGRMFVVKELIQTEKDYVKDLGIVVEGFMKRIEEKGVPDNMKGKDKIVFGNIHQIYDWHKDFFVGELEKCLEDHEKLPELFCKHERRLHMYVVYCQNKPKSEFIVAEYDSYFEGIQQDINSRLSISDFLIKPIQRITKYQLLLKDVLKYSTKAGLDCQDIENAVDLMSQVPKLCNDMMNLGRLQGYEGKLTSQGKLLQQETFFVTEQDSGVLSRSKERRVFLFEQIVIFSELLRKGSSTPGYQFKKSIKVSFLDLEEHVENDPCKFVLSCRGSAERFTLQAANTDIKQVWVQHITQLLDLQSNFLSALQSPIEYQKERSGSQSLTRNSSGRMGQSNSRPCSTVSLGAEKPSLSGRSSTPLKLSTSNGGPCSERYRRFEGLGCNGTSSSLMVTQDYTALKENEICVTQGEAVQVLATNQQNMYLVYRPANSQSPPAEGWVPGHILGPVTKPLIDTTADASIKKSLSWNTLRARKRAEKDSTPLKSDFKVENGLRKPKEILSSKVKVKESRSSDESECEDELDPKTSMELLNPNFIQEVAPEFLTPLTDVTCALGETVVLCCKVCARPKPTITLKGPDQSLLANNNRFTINIRETGDIVLKICNLMPQDTGIYTCVAVNDHGTASSSASIKVQGIPAAPARPVAQEASSTAVIVHWLPPASSANSAISSYTVEYRQEDSLVWQQLVVSTADVFVKIDNLIPGGHYQFRVSASNPWGTSPPSEPSNMVTLSSTASTYDGAGIQWKDNFESAFTELCEIGRGRFSVVRKCLSKATKQEVAVKFVNKKMHKKEQVAHEADILRHVQHPQLVSLIDTYESPTAYMLILELVEDGRLLDYLVAHDELMEEKVAFFIKDTLEALQHLHTCRVAHLDLKPENLLVDLHVPVPCVKLSDLGDAVQVSSHRYVHLLLGNPEFAAPELIQGTPVSLSTDVWSVGVLAYVMLSGVSPFLDESLEETCVNICRLDFCFPQEYFSDISQAAKDFIVSTLNQDPRKRPSSASCLQHPWVSAHSGDYSKTPLDTVRLAAFIDRRKQLHDVRPVTNIKGMVSSSMGHTL

Target ORF information:

RefSeq Version	XM_009304450.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kalirin, RhoGEF kinase a (kalrna), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304450.2

ORF Insert Sequence:

ATGAATTCTG CTGAAGCGCC GGAGGACGGA CCCAATGATT CGGACACGGA TGCATTCTTC 
AAAACAGGGT CTTTCAGAAG TGAAGGAATT AAAGCCTCTG ATGTCCTTCC TGTGCTGAGA 
GAAAAAATAG CATTTGTGTC AGGCGGTCGT GATAAGCGAG GTGGCCCTAT ACTGACCTTT 
CCTGCAAGGA GTAACCATGA CCGAATAAAA CAAGAGGACC TGAGACGGCT CGTAACATAC 
CTGTCCACTG TGCCCAGTGA GGACGTTTGT AAGAGAGGCT TCACTGTGAT TATTGATATG 
CGTGGCTCTA AATGGGAGCT AATAAAGCCA CTGCTGAAGA CGCTACAGGA AGCATTTCCA 
GCTGAGATCT GCGTCGCTCT CATCATCAAA CCAGACAACT TCTGGCAGAA GCAGAAGACT 
AACTTTGGTA GCGCCAAGTT CACCTTTGAG ACTAGCATGG TATCAGTGGA GGGCTTGACC 
AAACTAGTGG ACCCATCCCA GCTAACGGAC GACTTTGAAG GTTCTCTGGA ATACAATCAT 
GAGGAATGGC TTGAGCTCCG GGTGGCTCTG GAGGAGTTCC TGGCCAGCGC CGTTCACCTC 
CTCTCTCGCC TGGAAGATCT ACAGGAGACT CTGGCTAAGA AGGAGTTCCC GGTGGATGTG 
GAAGCCTCTC GTAGACTCAT CGATGAGCAC ACACAGCTGA AGAAGAAGGT GATGAAGGCT 
CCGGTGGAAG AGCTGGACCA CGAAGGCCAG AGACTCTTGC AGTGCATCCG CTCCAGCGAC 
GGCTTCTCAG GGAGGAACTG CATTTCTGGA AGTGCCGACT TCCAGAGCGT GGTTCCGAAG 
ATCGCCAGTC TGCTGGATAA ACTCCACAGC ACTCGACAGC ACTTGCACCA GATGTGGCAC 
CTACGAAAAC TCAAGCTGGA TCAGTGCTTT CAGCTCCGAC TGTTTGAACA GGACGCAGAG 
AAGATGTTTG ACTGGATCAG TCACAATAAG GAATTGTTTC TTCAGAGCCA CACGGAGATC 
GGTGTGAGCT ACCAGCATGC TGTGGAGCTA CAGACGCAAC ACAACCACTT TGCCATGAAC 
TCAATGAATG CCTATGTGAA CATAAACCGC ATCATGTCGG TGGCGTCGCG GCTGTCGGAG 
GCGGGCCACT ACGCCGCTCA GCAGATCAAA CAGATCAGCA CTCAGCTGGA TCAGGAGTGG 
AAGAGCTTCG CGGCTGCTCT CGATGAACGC AGCACCATCC TTGCCATGTC TGCCGTCTTC 
CATCAGAAAT CTGAACAGTT TTTGTCCGGT GTGGAAACAT GGTGTAAGAT GTGCAGTGAA 
GGCGGCTTGC CATCAGAGAT GCAGGATCTG GAATCGGCCA TTCATCACCA TCAGACGCTC 
TATGAACAAG TCACACAAGC ATACACCGAG GTGAGTCAGG ACGGTAAGGC TTTGCTGGAC 
GTTCTTCAGC GGCCGTTGAG TCCTGGGAAC GCAGAGTCAC TGACTGCGAC AGCGAACTAC 
TCTAAAGCCG TGCACTGTAT ACTGGACGTG GTCCACGAGG TCCTGCATCA TCAGAGACGT 
CTGGAGAACA TCTGGCAGCA CCGTAAAGTC CGTCTGCATC AGAGACTGCA GCTCTGTGTC 
TTCCAGCAGG ACGTCCAACA GGTGATTGAC TGGATAGAGA ATCACGGGGA GGCTTTCCTC 
AGCAAACACA CTGGTGTGGG CAAATCCCTG CACAGAGCCA GAGCCCTCCA GAAACGCCAT 
GATGACTTTG AGGAGGTGGC ACAGAATACA TACACTAATG CAGATAAGCT GCTGGAAGCT 
GCTGAGCAGT TGGCTCAGAC GGGCGAGTGT GACCCTGAGG AGATCTACAA GGCAGCGCGA 
CACCTGGAAG TCAGGATCCA GGACTTTGTT AGGCGAGTGG AGCAAAGAAA ACTACTGCTG 
GACATGTCTG TCTCTTTCCA CACGCACACT AAAGAGTTGT GGACGTGGAT GGAGGACCTG 
CAGAAAGAGC TGCTGGAGGA AGTTTGTTCT GACTCAGTCG ATTCAGTTCA GGAGCTGATA 
AAACAGTTTC AGCAGCAGCA GACGGCCACG CTGGAGGCAA CACTCAATGT CATTAAAGAG 
GGAGAAGATC TTATGCAACA ACTCCGAGAT TCAGCAATGA GCAGTAATAA GATGCCCCAC 
ACCAGCTCCA TCGCTCACAT TGAGGGCGTC CTGCAGCAGC TAGAGGAGGC GCAGGGCCAC 
ATGGAGGAAC TCTTCCATGA GAGAAAGATC AAACTCGACA TCTTCCTGCA ACTGCGCATC 
TTTGAGCAGT ACACCATTGA GGTAACGGCA GAGTTAGACG CGTGGAATGA AGACTTGGTT 
CGTCAGATGA ATGAGTATAC TGGAGAAGAA CCCAGTCTGG CTGAGCAGAG ACTCCAGCGG 
CACGCAGAGA GGAAACTGGC CATGACTAAC ATGACCTACG AAGTTATGCA GCAGGGTCAG 
GACCTGCATC AGTACATCAT GGAAGTCCAA GCTTCAGGTA TTGAGTTGAC CGGAGAGAAG 
GAGGTGGATC TGGCAACACA AGTTCAGGAG CTGCTGGAGT TCCTTCATGA GAAACAGCAC 
GAGTTGGACA TGAGCGCCGA ACACACACAG AAGCGCCTGG AGCAGTGTGT GCAGCTCCGC 
CACCTGCAGG CTGAGGTTAA ACAGGTTCTG GGGTGGATTC GTAATGGTGA ATCTATGTTG 
GATGCCAGTA TGGTGAATGC AAGTTCACTG TCAGAGGCCG AACAACTACA GAGAGAACAT 
GAACAGTTCC AGCTGGCCAT AGAGTCTTTG TTCCACGCTA ACTCTCTCCA GAAAACCCAT 
CAGAGTGCAC TACAGGTCCA GCAGAAGGCA GAAATCATGT TACAGGCTGG ACACTTTGAC 
CCCGAGGCCA TCCGCAGCTG TGCAGAGAAG GTGGCCGTGC ACTGGCAGCA GCTCATGCTC 
AAGATGGAGG ACAGACTCAA ACTGGTTAAT GCCTCGGTGG CCTTCTACAA GACGTCAGAA 
CAGGTGTGTA GTGTCCTGGA GAGTCTGGAG CAGGAGTACC GCAGAGATGA AGACTGGTGT 
GGAGCTCATG ATAAACTAGG ATCTTCATCA GAGACTGATC ACGTTCTGCC TCTCATCAGC 
AAACACCTGG AGCAGAAAGA AGCTTTTCTC AAGGCATGCA CTCTGGCCAG GCGCAATGCT 
GAGGTTTTTC TGAAATACAT CCACCGGAAC AATGTCAGCT CTCAGGGATC ATCTAGCAAC 
ACTCGTGGCC CTGAGCAACA GGTCAAAGCC ATCCTGAATG AACTGCTGCA GAGGGAAAAC 
AGAGTTTTGC ATTTCTGGAC CATGAAGAAG CGGCGTTTGG ATCAGTGTCA GCAGTATGTG 
GTGTTTGAAC GCAATGCCAA ACAGGCATTA GAGTGGATTG AGGAGGCAGG AGAGGTCTAT 
CTGGCCACTC ACACCTGCAT CGGAGACTCT ATGGAGAAAA CTCAGGGACT CCTCAAAGAA 
TACGAAGAAT TCTGCATCTC CGCAAAGCAA ACTAAGGAGA AAGTGAAGAT GCTGATCCAG 
CTGGCGGATA ACTTTGTTGA GAAAGGACAC ATGCATGTCA CCGACTTGAA GAAGTGTCTG 
ACGGCAGTGG ACAAGCGCTA CAGGGACTTC TCCATGCGCA TGGGCAAATA CCGCTGTGAC 
CTGGAAAATG TGCTGGGCAT CAGCTCTGAG GATAATAAGG ATCTGGAGCT GGATATCATC 
CCAGCCAGTC TGACTGGCCC AGAGGTTAAA CTCCGTGACC CCAACCACGA GGTGAATGAA 
GAAAAGAGGA AATCAGCCCG GAAGAAAGAG TTTATTATAG CAGAGCTGTT GCAGACAGAA 
AAGGCTTATG TGAGGGACTT ACATGAATGC TTAGAGACAT ATTTGTGGGA GATGACCAAT 
GGTGTGGAGG AAATTCCTCC AGGAATCGCC AACAAAGAGC ACATCATCTT TGGCAACATT 
CAGGAGATTT ACGACTTCCA CAATAATATA TTCCTGAAGG AGCTGATCAA TTATGAGCAA 
CTGCCTGAGG ATGTGGGACA TTGCTTTGTG ACATGGGCCG ATAAGTTTAA CATTTATGTG 
ACATACTGCA AAAACAAACC AGATTCCAGT CAGCTCATCC TAGACCATGC TGGGAGTTTT 
TTTGATGAGA TTCAGAAGAG ACACAGTCTG ACCAACTCTA TATCATCATA CCTCATCAAA 
CCTGTACAGA GGATCACCAA ATATCAGCTG CTGCTCAAGG AGCTGCTGTC CTGCTGTGAA 
GAAGGCAAAG GTGAAATTAA AGATGGCCTG GAGGTCATGC TGAGTGTGCC AAAGAGAGCC 
AATGACGCCA TGCACGTCAG CATGCTGGAG GGTTTTGATG CAAATTTGGA TGTTCAGGGT 
GAACTCATCC TCCAAGACAC CTTCCAGGTG TGGGACCCCA AATCACTAAT CCGGAAGGGG 
CGAGACCGCC ACCTCTTTCT CTTCGAGATC TCCCTGGTCT TCAGTAAAGA GATCAAAGAC 
TCAGCAGGGC GATCCAAATA CATCTTTAAA AACAAACTAC TGACTTCTGA ATTGGGGGTG 
ACAGAGCATG TAGAGGGGGA CCCGTGCAAA TTTGCTCTGT GGGTGGGACG CACACCATCG 
TCTGACAACA AGACTGTCCT CAAGGCATCT AGTATTGAGG TGAAGCAAGA ATGGATTAAA 
AACATCCGAG AGGTCATTCA GGAGAGAATA ATTCACCTGA AGGGGGCGCT AAAGGAACCT 
ATCCACATTC CCAAAACTCC AGCCAAACCT CGCAATAACA GCAAGAGGGA CGCTGGTGAG 
GATGCTGACA GCCAGGGAGA CGGCAGCAGT CAGCCGGACA CCATCTCCAT AGCATCCAGA 
ACCTCTCAGA ACACTGTAGA CAGTGACAAG CTCTCTGGAG GGTGTGAGCT GACGGTGGTC 
CTGCAGGACT TTACAGCAAG CAGCTCATCA GAGCTCAGCA TCCAGACGGG TCAGACAGTG 
GAGCTGCTGG AGCGGCCCAG TGACCGTCCA GGATGGTGTC TGGTACGGAC CACCGAAAAG 
ACGCCTCCTC AAGAGGGACT GGTGCCCAGC AGTGCTCTGT GTATCTCGCA CTCACGCAGC 
AGTGTGGAGA TGGACTGCTT TTTCCCAGCA GGCAAAGAAA ACTATACAGT TAGTGGCCCA 
GAAGGAAAGA CAGAGTCAGT GGCCACACTG CAGCCACAGC CATCCCTGAA CTCTCTGCAG 
AGTAGCTCTC CTGGACCCAA GCGCTCTGGG AATACACTGA AGAAATGGCT GACCAGTCCT 
GTCCGACGTC TAAGTCATGG CAGTAACATC AAGAAGATCC CAAGTAAACA GAAACAGAAA 
CGTGATGGTC GCAAGAGTAT TGATATGGGG CCGCCCGAAC TACAGGATGA CACCTTGGAA 
GAGCGAGTGC AAAAAGAAGA GGGCACACTC TCCAAGTCTT CGTCTGGGAT GCACAGTGGA 
GGAGAGGAAG AGCCTGAGGA CGAGTCTCAC ACTCCTCTAC CTCCACCAAT GGAGATCATT 
AAAGACCCGT CGGCTCAGGA AGAGAAGTCG TCTGTGCTTC TGGCTTCCCG GCAGAGCTCG 
GCTGATGTCC CCAGTGCTGC TGAGCTTGTC AGTGCTATAG AAAAACTGGT CAAAACTAAA 
ATGACACTGG AGCCAGGCTC ATACCCGGGA TTCAGCTCAG TCAATCCAGC AGAGCAGAGC 
CCGATTCCAC CCAGAAACCC AGAACTAGAG CAGAAAGCCA AAGCGCTACG AGGACGAATG 
TTTGTAGTCA AGGAATTAAT CCAAACGGAA AAAGACTATG TTAAAGATCT GGGAATTGTG 
GTTGAGGGGT TTATGAAGAG AATCGAGGAG AAAGGAGTTC CTGATAACAT GAAAGGAAAA 
GATAAGATCG TCTTCGGAAA CATTCACCAA ATCTATGACT GGCACAAAGA TTTTTTTGTT 
GGAGAACTGG AGAAATGCCT GGAAGATCAC GAAAAACTGC CTGAACTGTT CTGTAAACAT 
GAGAGACGGT TACACATGTA TGTAGTTTAC TGTCAGAACA AACCCAAATC CGAGTTCATC 
GTTGCAGAAT ATGATTCATA CTTTGAGGGG ATTCAGCAAG ACATTAACTC CAGACTGAGC 
ATCAGCGATT TTCTCATTAA ACCCATTCAG AGAATTACTA AATACCAGCT TTTATTAAAG 
GATGTTTTGA AGTACAGTAC AAAAGCAGGA CTGGATTGTC AGGATATAGA GAATGCAGTT 
GACCTGATGT CCCAGGTTCC CAAGCTTTGC AATGACATGA TGAATCTGGG AAGACTGCAG 
GGATATGAGG GGAAGTTGAC GAGTCAAGGA AAACTCTTGC AGCAGGAGAC GTTCTTTGTG 
ACCGAGCAGG ACTCTGGCGT TCTGTCCCGC AGTAAAGAGC GCAGGGTTTT CCTGTTCGAA 
CAAATTGTCA TCTTCAGCGA GCTGTTACGT AAAGGCTCTT CCACTCCAGG GTACCAGTTC 
AAGAAGAGCA TAAAGGTGAG CTTCCTTGAC TTGGAAGAGC ATGTTGAAAA TGATCCATGC 
AAGTTTGTGC TGTCGTGTCG TGGGTCGGCA GAACGTTTTA CCCTTCAAGC AGCAAACACG 
GACATTAAAC AAGTCTGGGT TCAGCATATC ACCCAACTAC TAGATTTACA AAGCAACTTC 
TTGTCAGCTC TGCAGTCTCC TATTGAGTAT CAGAAGGAGC GTAGTGGCTC TCAATCTCTG 
ACCCGGAACT CTTCTGGGCG GATGGGTCAG TCCAACAGCA GGCCCTGCTC CACGGTTTCA 
CTCGGAGCAG AGAAACCCTC TCTGTCTGGA CGGAGCTCCA CCCCGCTAAA GCTATCTACC 
TCCAATGGTG GTCCATGCTC TGAAAGATAC CGCAGGTTTG AAGGCTTGGG CTGCAACGGT 
ACATCGTCCT CCTTGATGGT GACTCAAGAT TATACTGCAC TGAAAGAGAA TGAGATCTGT 
GTCACACAGG GTGAGGCCGT GCAGGTACTG GCCACTAACC AGCAGAACAT GTACCTTGTG 
TACCGACCCG CAAATAGCCA ATCACCACCT GCAGAGGGCT GGGTGCCGGG ACACATCCTA 
GGCCCCGTCA CAAAACCTCT CATAGACACT ACTGCAGACG CAAGCATCAA GAAATCTCTT 
TCGTGGAACA CACTTCGTGC TCGTAAGCGT GCAGAAAAGG ACAGCACGCC CCTCAAAAGT 
GACTTTAAAG TTGAGAATGG ACTGCGTAAG CCAAAGGAAA TTCTCAGCAG CAAAGTCAAA 
GTGAAAGAGT CCCGCAGTTC TGATGAGTCT GAATGTGAGG ATGAACTAGA CCCCAAAACT 
AGTATGGAGT TACTGAACCC TAATTTCATC CAAGAGGTCG CTCCAGAGTT TCTGACGCCT 
CTAACAGACG TGACGTGTGC TCTGGGAGAG ACGGTGGTTC TGTGCTGTAA AGTGTGTGCT 
CGACCCAAAC CCACAATCAC CTTGAAAGGA CCTGATCAAA GTCTGCTGGC CAACAATAAC 
CGCTTCACTA TTAATATCAG AGAAACCGGT GATATAGTGT TGAAAATCTG CAATCTAATG 
CCGCAGGACA CTGGCATCTA CACGTGTGTT GCTGTGAATG ACCATGGTAC AGCCTCCTCA 
TCTGCATCCA TTAAAGTACA AGGTATCCCA GCAGCCCCTG CTCGACCGGT GGCACAGGAG 
GCAAGCAGCA CTGCAGTGAT TGTCCACTGG CTTCCTCCAG CCAGCTCAGC AAATTCTGCC 
ATCTCCAGCT ACACTGTGGA ATATAGGCAG GAAGATTCTC TGGTATGGCA GCAGTTGGTG 
GTCTCCACGG CAGATGTGTT TGTGAAGATT GACAATCTGA TTCCTGGTGG GCACTACCAG 
TTCAGAGTCA GTGCCAGTAA TCCCTGGGGC ACCAGCCCGC CTAGTGAACC CTCAAACATG 
GTGACATTAT CCAGCACAGC ATCAACATAT GATGGCGCTG GAATCCAGTG GAAAGACAAC 
TTTGAATCTG CTTTTACAGA ATTATGTGAA ATTGGGCGGG GTCGTTTCTC TGTGGTGAGG 
AAGTGTCTCA GTAAAGCCAC TAAGCAGGAG GTTGCTGTGA AGTTTGTGAA TAAGAAGATG 
CACAAGAAAG AACAGGTGGC CCATGAAGCT GATATTCTAC GCCATGTCCA GCACCCCCAA 
CTGGTGTCAC TGATTGATAC ATACGAGTCG CCTACAGCCT ACATGCTGAT TCTTGAACTT 
GTTGAGGATG GACGTCTTCT TGACTATCTG GTAGCTCATG ATGAGCTGAT GGAGGAGAAA 
GTGGCATTCT TTATTAAAGA TACACTGGAA GCTCTGCAGC ACTTGCACAC CTGCAGAGTG 
GCTCATCTAG ACCTCAAGCC TGAAAACTTG TTGGTGGACC TCCACGTTCC GGTACCCTGC 
GTGAAGCTCT CAGATCTAGG TGATGCTGTA CAGGTTTCAA GTCACCGCTA CGTACATCTC 
CTCCTGGGTA ACCCCGAGTT CGCTGCTCCA GAGCTCATCC AGGGCACTCC AGTGTCCCTC 
TCTACAGATG TGTGGAGTGT AGGGGTGCTG GCCTACGTCA TGCTTAGTGG AGTCTCCCCA 
TTCCTGGATG AGAGTCTGGA GGAGACCTGC GTCAACATCT GCAGGCTGGA CTTCTGCTTT 
CCTCAAGAGT ACTTCTCCGA TATAAGCCAG GCAGCCAAAG ACTTCATTGT GTCCACGCTT 
AACCAGGATC CCAGGAAGAG GCCAAGCTCG GCCTCGTGTC TTCAGCACCC ATGGGTCAGT 
GCTCATAGTG GAGATTACTC CAAAACACCT CTGGATACTG TCAGACTCGC TGCATTTATA 
GACAGACGCA AACAGCTACA TGATGTCAGG CCTGTCACAA ACATTAAAGG AATGGTCAGC 
AGCAGTATGG GACACACCTT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kalrna ( XM_009304450.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302725.2 Danio rerio kalirin RhoGEF kinase a (kalrna), transcript variant X2, mRNA.

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CloneID	ODa60288
Clone ID Related Accession (Same CDS sequence)	XM_009304450.2 , XM_009304450.3
Accession Version	XM_009304450.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8904bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kalirin isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304450.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTG CTGAAGCGCC GGAGGACGGA CCCAATGATT CGGACACGGA TGCATTCTTC 
AAAACAGGGT CTTTCAGAAG TGAAGGAATT AAAGCCTCTG ATGTCCTTCC TGTGCTGAGA 
GAAAAAATAG CATTTGTGTC AGGCGGTCGT GATAAGCGAG GTGGCCCTAT ACTGACCTTT 
CCTGCAAGGA GTAACCATGA CCGAATAAAA CAAGAGGACC TGAGACGGCT CGTAACATAC 
CTGTCCACTG TGCCCAGTGA GGACGTTTGT AAGAGAGGCT TCACTGTGAT TATTGATATG 
CGTGGCTCTA AATGGGAGCT AATAAAGCCA CTGCTGAAGA CGCTACAGGA AGCATTTCCA 
GCTGAGATCT GCGTCGCTCT CATCATCAAA CCAGACAACT TCTGGCAGAA GCAGAAGACT 
AACTTTGGTA GCGCCAAGTT CACCTTTGAG ACTAGCATGG TATCAGTGGA GGGCTTGACC 
AAACTAGTGG ACCCATCCCA GCTAACGGAC GACTTTGAAG GTTCTCTGGA ATACAATCAT 
GAGGAATGGC TTGAGCTCCG GGTGGCTCTG GAGGAGTTCC TGGCCAGCGC CGTTCACCTC 
CTCTCTCGCC TGGAAGATCT ACAGGAGACT CTGGCTAAGA AGGAGTTCCC GGTGGATGTG 
GAAGCCTCTC GTAGACTCAT CGATGAGCAC ACACAGCTGA AGAAGAAGGT GATGAAGGCT 
CCGGTGGAAG AGCTGGACCA CGAAGGCCAG AGACTCTTGC AGTGCATCCG CTCCAGCGAC 
GGCTTCTCAG GGAGGAACTG CATTTCTGGA AGTGCCGACT TCCAGAGCGT GGTTCCGAAG 
ATCGCCAGTC TGCTGGATAA ACTCCACAGC ACTCGACAGC ACTTGCACCA GATGTGGCAC 
CTACGAAAAC TCAAGCTGGA TCAGTGCTTT CAGCTCCGAC TGTTTGAACA GGACGCAGAG 
AAGATGTTTG ACTGGATCAG TCACAATAAG GAATTGTTTC TTCAGAGCCA CACGGAGATC 
GGTGTGAGCT ACCAGCATGC TGTGGAGCTA CAGACGCAAC ACAACCACTT TGCCATGAAC 
TCAATGAATG CCTATGTGAA CATAAACCGC ATCATGTCGG TGGCGTCGCG GCTGTCGGAG 
GCGGGCCACT ACGCCGCTCA GCAGATCAAA CAGATCAGCA CTCAGCTGGA TCAGGAGTGG 
AAGAGCTTCG CGGCTGCTCT CGATGAACGC AGCACCATCC TTGCCATGTC TGCCGTCTTC 
CATCAGAAAT CTGAACAGTT TTTGTCCGGT GTGGAAACAT GGTGTAAGAT GTGCAGTGAA 
GGCGGCTTGC CATCAGAGAT GCAGGATCTG GAATCGGCCA TTCATCACCA TCAGACGCTC 
TATGAACAAG TCACACAAGC ATACACCGAG GTGAGTCAGG ACGGTAAGGC TTTGCTGGAC 
GTTCTTCAGC GGCCGTTGAG TCCTGGGAAC GCAGAGTCAC TGACTGCGAC AGCGAACTAC 
TCTAAAGCCG TGCACTGTAT ACTGGACGTG GTCCACGAGG TCCTGCATCA TCAGAGACGT 
CTGGAGAACA TCTGGCAGCA CCGTAAAGTC CGTCTGCATC AGAGACTGCA GCTCTGTGTC 
TTCCAGCAGG ACGTCCAACA GGTGATTGAC TGGATAGAGA ATCACGGGGA GGCTTTCCTC 
AGCAAACACA CTGGTGTGGG CAAATCCCTG CACAGAGCCA GAGCCCTCCA GAAACGCCAT 
GATGACTTTG AGGAGGTGGC ACAGAATACA TACACTAATG CAGATAAGCT GCTGGAAGCT 
GCTGAGCAGT TGGCTCAGAC GGGCGAGTGT GACCCTGAGG AGATCTACAA GGCAGCGCGA 
CACCTGGAAG TCAGGATCCA GGACTTTGTT AGGCGAGTGG AGCAAAGAAA ACTACTGCTG 
GACATGTCTG TCTCTTTCCA CACGCACACT AAAGAGTTGT GGACGTGGAT GGAGGACCTG 
CAGAAAGAGC TGCTGGAGGA AGTTTGTTCT GACTCAGTCG ATTCAGTTCA GGAGCTGATA 
AAACAGTTTC AGCAGCAGCA GACGGCCACG CTGGAGGCAA CACTCAATGT CATTAAAGAG 
GGAGAAGATC TTATGCAACA ACTCCGAGAT TCAGCAATGA GCAGTAATAA GATGCCCCAC 
ACCAGCTCCA TCGCTCACAT TGAGGGCGTC CTGCAGCAGC TAGAGGAGGC GCAGGGCCAC 
ATGGAGGAAC TCTTCCATGA GAGAAAGATC AAACTCGACA TCTTCCTGCA ACTGCGCATC 
TTTGAGCAGT ACACCATTGA GGTAACGGCA GAGTTAGACG CGTGGAATGA AGACTTGGTT 
CGTCAGATGA ATGAGTATAC TGGAGAAGAA CCCAGTCTGG CTGAGCAGAG ACTCCAGCGG 
CACGCAGAGA GGAAACTGGC CATGACTAAC ATGACCTACG AAGTTATGCA GCAGGGTCAG 
GACCTGCATC AGTACATCAT GGAAGTCCAA GCTTCAGGTA TTGAGTTGAC CGGAGAGAAG 
GAGGTGGATC TGGCAACACA AGTTCAGGAG CTGCTGGAGT TCCTTCATGA GAAACAGCAC 
GAGTTGGACA TGAGCGCCGA ACACACACAG AAGCGCCTGG AGCAGTGTGT GCAGCTCCGC 
CACCTGCAGG CTGAGGTTAA ACAGGTTCTG GGGTGGATTC GTAATGGTGA ATCTATGTTG 
GATGCCAGTA TGGTGAATGC AAGTTCACTG TCAGAGGCCG AACAACTACA GAGAGAACAT 
GAACAGTTCC AGCTGGCCAT AGAGTCTTTG TTCCACGCTA ACTCTCTCCA GAAAACCCAT 
CAGAGTGCAC TACAGGTCCA GCAGAAGGCA GAAATCATGT TACAGGCTGG ACACTTTGAC 
CCCGAGGCCA TCCGCAGCTG TGCAGAGAAG GTGGCCGTGC ACTGGCAGCA GCTCATGCTC 
AAGATGGAGG ACAGACTCAA ACTGGTTAAT GCCTCGGTGG CCTTCTACAA GACGTCAGAA 
CAGGTGTGTA GTGTCCTGGA GAGTCTGGAG CAGGAGTACC GCAGAGATGA AGACTGGTGT 
GGAGCTCATG ATAAACTAGG ATCTTCATCA GAGACTGATC ACGTTCTGCC TCTCATCAGC 
AAACACCTGG AGCAGAAAGA AGCTTTTCTC AAGGCATGCA CTCTGGCCAG GCGCAATGCT 
GAGGTTTTTC TGAAATACAT CCACCGGAAC AATGTCAGCT CTCAGGGATC ATCTAGCAAC 
ACTCGTGGCC CTGAGCAACA GGTCAAAGCC ATCCTGAATG AACTGCTGCA GAGGGAAAAC 
AGAGTTTTGC ATTTCTGGAC CATGAAGAAG CGGCGTTTGG ATCAGTGTCA GCAGTATGTG 
GTGTTTGAAC GCAATGCCAA ACAGGCATTA GAGTGGATTG AGGAGGCAGG AGAGGTCTAT 
CTGGCCACTC ACACCTGCAT CGGAGACTCT ATGGAGAAAA CTCAGGGACT CCTCAAAGAA 
TACGAAGAAT TCTGCATCTC CGCAAAGCAA ACTAAGGAGA AAGTGAAGAT GCTGATCCAG 
CTGGCGGATA ACTTTGTTGA GAAAGGACAC ATGCATGTCA CCGACTTGAA GAAGTGTCTG 
ACGGCAGTGG ACAAGCGCTA CAGGGACTTC TCCATGCGCA TGGGCAAATA CCGCTGTGAC 
CTGGAAAATG TGCTGGGCAT CAGCTCTGAG GATAATAAGG ATCTGGAGCT GGATATCATC 
CCAGCCAGTC TGACTGGCCC AGAGGTTAAA CTCCGTGACC CCAACCACGA GGTGAATGAA 
GAAAAGAGGA AATCAGCCCG GAAGAAAGAG TTTATTATAG CAGAGCTGTT GCAGACAGAA 
AAGGCTTATG TGAGGGACTT ACATGAATGC TTAGAGACAT ATTTGTGGGA GATGACCAAT 
GGTGTGGAGG AAATTCCTCC AGGAATCGCC AACAAAGAGC ACATCATCTT TGGCAACATT 
CAGGAGATTT ACGACTTCCA CAATAATATA TTCCTGAAGG AGCTGATCAA TTATGAGCAA 
CTGCCTGAGG ATGTGGGACA TTGCTTTGTG ACATGGGCCG ATAAGTTTAA CATTTATGTG 
ACATACTGCA AAAACAAACC AGATTCCAGT CAGCTCATCC TAGACCATGC TGGGAGTTTT 
TTTGATGAGA TTCAGAAGAG ACACAGTCTG ACCAACTCTA TATCATCATA CCTCATCAAA 
CCTGTACAGA GGATCACCAA ATATCAGCTG CTGCTCAAGG AGCTGCTGTC CTGCTGTGAA 
GAAGGCAAAG GTGAAATTAA AGATGGCCTG GAGGTCATGC TGAGTGTGCC AAAGAGAGCC 
AATGACGCCA TGCACGTCAG CATGCTGGAG GGTTTTGATG CAAATTTGGA TGTTCAGGGT 
GAACTCATCC TCCAAGACAC CTTCCAGGTG TGGGACCCCA AATCACTAAT CCGGAAGGGG 
CGAGACCGCC ACCTCTTTCT CTTCGAGATC TCCCTGGTCT TCAGTAAAGA GATCAAAGAC 
TCAGCAGGGC GATCCAAATA CATCTTTAAA AACAAACTAC TGACTTCTGA ATTGGGGGTG 
ACAGAGCATG TAGAGGGGGA CCCGTGCAAA TTTGCTCTGT GGGTGGGACG CACACCATCG 
TCTGACAACA AGACTGTCCT CAAGGCATCT AGTATTGAGG TGAAGCAAGA ATGGATTAAA 
AACATCCGAG AGGTCATTCA GGAGAGAATA ATTCACCTGA AGGGGGCGCT AAAGGAACCT 
ATCCACATTC CCAAAACTCC AGCCAAACCT CGCAATAACA GCAAGAGGGA CGCTGGTGAG 
GATGCTGACA GCCAGGGAGA CGGCAGCAGT CAGCCGGACA CCATCTCCAT AGCATCCAGA 
ACCTCTCAGA ACACTGTAGA CAGTGACAAG CTCTCTGGAG GGTGTGAGCT GACGGTGGTC 
CTGCAGGACT TTACAGCAAG CAGCTCATCA GAGCTCAGCA TCCAGACGGG TCAGACAGTG 
GAGCTGCTGG AGCGGCCCAG TGACCGTCCA GGATGGTGTC TGGTACGGAC CACCGAAAAG 
ACGCCTCCTC AAGAGGGACT GGTGCCCAGC AGTGCTCTGT GTATCTCGCA CTCACGCAGC 
AGTGTGGAGA TGGACTGCTT TTTCCCAGCA GGCAAAGAAA ACTATACAGT TAGTGGCCCA 
GAAGGAAAGA CAGAGTCAGT GGCCACACTG CAGCCACAGC CATCCCTGAA CTCTCTGCAG 
AGTAGCTCTC CTGGACCCAA GCGCTCTGGG AATACACTGA AGAAATGGCT GACCAGTCCT 
GTCCGACGTC TAAGTCATGG CAGTAACATC AAGAAGATCC CAAGTAAACA GAAACAGAAA 
CGTGATGGTC GCAAGAGTAT TGATATGGGG CCGCCCGAAC TACAGGATGA CACCTTGGAA 
GAGCGAGTGC AAAAAGAAGA GGGCACACTC TCCAAGTCTT CGTCTGGGAT GCACAGTGGA 
GGAGAGGAAG AGCCTGAGGA CGAGTCTCAC ACTCCTCTAC CTCCACCAAT GGAGATCATT 
AAAGACCCGT CGGCTCAGGA AGAGAAGTCG TCTGTGCTTC TGGCTTCCCG GCAGAGCTCG 
GCTGATGTCC CCAGTGCTGC TGAGCTTGTC AGTGCTATAG AAAAACTGGT CAAAACTAAA 
ATGACACTGG AGCCAGGCTC ATACCCGGGA TTCAGCTCAG TCAATCCAGC AGAGCAGAGC 
CCGATTCCAC CCAGAAACCC AGAACTAGAG CAGAAAGCCA AAGCGCTACG AGGACGAATG 
TTTGTAGTCA AGGAATTAAT CCAAACGGAA AAAGACTATG TTAAAGATCT GGGAATTGTG 
GTTGAGGGGT TTATGAAGAG AATCGAGGAG AAAGGAGTTC CTGATAACAT GAAAGGAAAA 
GATAAGATCG TCTTCGGAAA CATTCACCAA ATCTATGACT GGCACAAAGA TTTTTTTGTT 
GGAGAACTGG AGAAATGCCT GGAAGATCAC GAAAAACTGC CTGAACTGTT CTGTAAACAT 
GAGAGACGGT TACACATGTA TGTAGTTTAC TGTCAGAACA AACCCAAATC CGAGTTCATC 
GTTGCAGAAT ATGATTCATA CTTTGAGGGG ATTCAGCAAG ACATTAACTC CAGACTGAGC 
ATCAGCGATT TTCTCATTAA ACCCATTCAG AGAATTACTA AATACCAGCT TTTATTAAAG 
GATGTTTTGA AGTACAGTAC AAAAGCAGGA CTGGATTGTC AGGATATAGA GAATGCAGTT 
GACCTGATGT CCCAGGTTCC CAAGCTTTGC AATGACATGA TGAATCTGGG AAGACTGCAG 
GGATATGAGG GGAAGTTGAC GAGTCAAGGA AAACTCTTGC AGCAGGAGAC GTTCTTTGTG 
ACCGAGCAGG ACTCTGGCGT TCTGTCCCGC AGTAAAGAGC GCAGGGTTTT CCTGTTCGAA 
CAAATTGTCA TCTTCAGCGA GCTGTTACGT AAAGGCTCTT CCACTCCAGG GTACCAGTTC 
AAGAAGAGCA TAAAGGTGAG CTTCCTTGAC TTGGAAGAGC ATGTTGAAAA TGATCCATGC 
AAGTTTGTGC TGTCGTGTCG TGGGTCGGCA GAACGTTTTA CCCTTCAAGC AGCAAACACG 
GACATTAAAC AAGTCTGGGT TCAGCATATC ACCCAACTAC TAGATTTACA AAGCAACTTC 
TTGTCAGCTC TGCAGTCTCC TATTGAGTAT CAGAAGGAGC GTAGTGGCTC TCAATCTCTG 
ACCCGGAACT CTTCTGGGCG GATGGGTCAG TCCAACAGCA GGCCCTGCTC CACGGTTTCA 
CTCGGAGCAG AGAAACCCTC TCTGTCTGGA CGGAGCTCCA CCCCGCTAAA GCTATCTACC 
TCCAATGGTG GTCCATGCTC TGAAAGATAC CGCAGGTTTG AAGGCTTGGG CTGCAACGGT 
ACATCGTCCT CCTTGATGGT GACTCAAGAT TATACTGCAC TGAAAGAGAA TGAGATCTGT 
GTCACACAGG GTGAGGCCGT GCAGGTACTG GCCACTAACC AGCAGAACAT GTACCTTGTG 
TACCGACCCG CAAATAGCCA ATCACCACCT GCAGAGGGCT GGGTGCCGGG ACACATCCTA 
GGCCCCGTCA CAAAACCTCT CATAGACACT ACTGCAGACG CAAGCATCAA GAAATCTCTT 
TCGTGGAACA CACTTCGTGC TCGTAAGCGT GCAGAAAAGG ACAGCACGCC CCTCAAAAGT 
GACTTTAAAG TTGAGAATGG ACTGCGTAAG CCAAAGGAAA TTCTCAGCAG CAAAGTCAAA 
GTGAAAGAGT CCCGCAGTTC TGATGAGTCT GAATGTGAGG ATGAACTAGA CCCCAAAACT 
AGTATGGAGT TACTGAACCC TAATTTCATC CAAGAGGTCG CTCCAGAGTT TCTGACGCCT 
CTAACAGACG TGACGTGTGC TCTGGGAGAG ACGGTGGTTC TGTGCTGTAA AGTGTGTGCT 
CGACCCAAAC CCACAATCAC CTTGAAAGGA CCTGATCAAA GTCTGCTGGC CAACAATAAC 
CGCTTCACTA TTAATATCAG AGAAACCGGT GATATAGTGT TGAAAATCTG CAATCTAATG 
CCGCAGGACA CTGGCATCTA CACGTGTGTT GCTGTGAATG ACCATGGTAC AGCCTCCTCA 
TCTGCATCCA TTAAAGTACA AGGTATCCCA GCAGCCCCTG CTCGACCGGT GGCACAGGAG 
GCAAGCAGCA CTGCAGTGAT TGTCCACTGG CTTCCTCCAG CCAGCTCAGC AAATTCTGCC 
ATCTCCAGCT ACACTGTGGA ATATAGGCAG GAAGATTCTC TGGTATGGCA GCAGTTGGTG 
GTCTCCACGG CAGATGTGTT TGTGAAGATT GACAATCTGA TTCCTGGTGG GCACTACCAG 
TTCAGAGTCA GTGCCAGTAA TCCCTGGGGC ACCAGCCCGC CTAGTGAACC CTCAAACATG 
GTGACATTAT CCAGCACAGC ATCAACATAT GATGGCGCTG GAATCCAGTG GAAAGACAAC 
TTTGAATCTG CTTTTACAGA ATTATGTGAA ATTGGGCGGG GTCGTTTCTC TGTGGTGAGG 
AAGTGTCTCA GTAAAGCCAC TAAGCAGGAG GTTGCTGTGA AGTTTGTGAA TAAGAAGATG 
CACAAGAAAG AACAGGTGGC CCATGAAGCT GATATTCTAC GCCATGTCCA GCACCCCCAA 
CTGGTGTCAC TGATTGATAC ATACGAGTCG CCTACAGCCT ACATGCTGAT TCTTGAACTT 
GTTGAGGATG GACGTCTTCT TGACTATCTG GTAGCTCATG ATGAGCTGAT GGAGGAGAAA 
GTGGCATTCT TTATTAAAGA TACACTGGAA GCTCTGCAGC ACTTGCACAC CTGCAGAGTG 
GCTCATCTAG ACCTCAAGCC TGAAAACTTG TTGGTGGACC TCCACGTTCC GGTACCCTGC 
GTGAAGCTCT CAGATCTAGG TGATGCTGTA CAGGTTTCAA GTCACCGCTA CGTACATCTC 
CTCCTGGGTA ACCCCGAGTT CGCTGCTCCA GAGCTCATCC AGGGCACTCC AGTGTCCCTC 
TCTACAGATG TGTGGAGTGT AGGGGTGCTG GCCTACGTCA TGCTTAGTGG AGTCTCCCCA 
TTCCTGGATG AGAGTCTGGA GGAGACCTGC GTCAACATCT GCAGGCTGGA CTTCTGCTTT 
CCTCAAGAGT ACTTCTCCGA TATAAGCCAG GCAGCCAAAG ACTTCATTGT GTCCACGCTT 
AACCAGGATC CCAGGAAGAG GCCAAGCTCG GCCTCGTGTC TTCAGCACCC ATGGGTCAGT 
GCTCATAGTG GAGATTACTC CAAAACACCT CTGGATACTG TCAGACTCGC TGCATTTATA 
GACAGACGCA AACAGCTACA TGATGTCAGG CCTGTCACAA ACATTAAAGG AATGGTCAGC 
AGCAGTATGG GACACACCTT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302725.2
CDS	554..9457
Translation	MNSAEAPEDGPNDSDTDAFFKTGSFRSEGIKASDVLPVLREKIAFVSGGRDKRGGPILTFPARSNHDRIKQEDLRRLVTYLSTVPSEDVCKRGFTVIIDMRGSKWELIKPLLKTLQEAFPAEICVALIIKPDNFWQKQKTNFGSAKFTFETSMVSVEGLTKLVDPSQLTDDFEGSLEYNHEEWLELRVALEEFLASAVHLLSRLEDLQETLAKKEFPVDVEASRRLIDEHTQLKKKVMKAPVEELDHEGQRLLQCIRSSDGFSGRNCISGSADFQSVVPKIASLLDKLHSTRQHLHQMWHLRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHAVELQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYAAQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKSEQFLSGVETWCKMCSEGGLPSEMQDLESAIHHHQTLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNAESLTATANYSKAVHCILDVVHEVLHHQRRLENIWQHRKVRLHQRLQLCVFQQDVQQVIDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKELLEEVCSDSVDSVQELIKQFQQQQTATLEATLNVIKEGEDLMQQLRDSAMSSNKMPHTSSIAHIEGVLQQLEEAQGHMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLVRQMNEYTGEEPSLAEQRLQRHAERKLAMTNMTYEVMQQGQDLHQYIMEVQASGIELTGEKEVDLATQVQELLEFLHEKQHELDMSAEHTQKRLEQCVQLRHLQAEVKQVLGWIRNGESMLDASMVNASSLSEAEQLQREHEQFQLAIESLFHANSLQKTHQSALQVQQKAEIMLQAGHFDPEAIRSCAEKVAVHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGAHDKLGSSSETDHVLPLISKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSSQGSSSNTRGPEQQVKAILNELLQRENRVLHFWTMKKRRLDQCQQYVVFERNAKQALEWIEEAGEVYLATHTCIGDSMEKTQGLLKEYEEFCISAKQTKEKVKMLIQLADNFVEKGHMHVTDLKKCLTAVDKRYRDFSMRMGKYRCDLENVLGISSEDNKDLELDIIPASLTGPEVKLRDPNHEVNEEKRKSARKKEFIIAELLQTEKAYVRDLHECLETYLWEMTNGVEEIPPGIANKEHIIFGNIQEIYDFHNNIFLKELINYEQLPEDVGHCFVTWADKFNIYVTYCKNKPDSSQLILDHAGSFFDEIQKRHSLTNSISSYLIKPVQRITKYQLLLKELLSCCEEGKGEIKDGLEVMLSVPKRANDAMHVSMLEGFDANLDVQGELILQDTFQVWDPKSLIRKGRDRHLFLFEISLVFSKEIKDSAGRSKYIFKNKLLTSELGVTEHVEGDPCKFALWVGRTPSSDNKTVLKASSIEVKQEWIKNIREVIQERIIHLKGALKEPIHIPKTPAKPRNNSKRDAGEDADSQGDGSSQPDTISIASRTSQNTVDSDKLSGGCELTVVLQDFTASSSSELSIQTGQTVELLERPSDRPGWCLVRTTEKTPPQEGLVPSSALCISHSRSSVEMDCFFPAGKENYTVSGPEGKTESVATLQPQPSLNSLQSSSPGPKRSGNTLKKWLTSPVRRLSHGSNIKKIPSKQKQKRDGRKSIDMGPPELQDDTLEERVQKEEGTLSKSSSGMHSGGEEEPEDESHTPLPPPMEIIKDPSAQEEKSSVLLASRQSSADVPSAAELVSAIEKLVKTKMTLEPGSYPGFSSVNPAEQSPIPPRNPELEQKAKALRGRMFVVKELIQTEKDYVKDLGIVVEGFMKRIEEKGVPDNMKGKDKIVFGNIHQIYDWHKDFFVGELEKCLEDHEKLPELFCKHERRLHMYVVYCQNKPKSEFIVAEYDSYFEGIQQDINSRLSISDFLIKPIQRITKYQLLLKDVLKYSTKAGLDCQDIENAVDLMSQVPKLCNDMMNLGRLQGYEGKLTSQGKLLQQETFFVTEQDSGVLSRSKERRVFLFEQIVIFSELLRKGSSTPGYQFKKSIKVSFLDLEEHVENDPCKFVLSCRGSAERFTLQAANTDIKQVWVQHITQLLDLQSNFLSALQSPIEYQKERSGSQSLTRNSSGRMGQSNSRPCSTVSLGAEKPSLSGRSSTPLKLSTSNGGPCSERYRRFEGLGCNGTSSSLMVTQDYTALKENEICVTQGEAVQVLATNQQNMYLVYRPANSQSPPAEGWVPGHILGPVTKPLIDTTADASIKKSLSWNTLRARKRAEKDSTPLKSDFKVENGLRKPKEILSSKVKVKESRSSDESECEDELDPKTSMELLNPNFIQEVAPEFLTPLTDVTCALGETVVLCCKVCARPKPTITLKGPDQSLLANNNRFTINIRETGDIVLKICNLMPQDTGIYTCVAVNDHGTASSSASIKVQGIPAAPARPVAQEASSTAVIVHWLPPASSANSAISSYTVEYRQEDSLVWQQLVVSTADVFVKIDNLIPGGHYQFRVSASNPWGTSPPSEPSNMVTLSSTASTYDGAGIQWKDNFESAFTELCEIGRGRFSVVRKCLSKATKQEVAVKFVNKKMHKKEQVAHEADILRHVQHPQLVSLIDTYESPTAYMLILELVEDGRLLDYLVAHDELMEEKVAFFIKDTLEALQHLHTCRVAHLDLKPENLLVDLHVPVPCVKLSDLGDAVQVSSHRYVHLLLGNPEFAAPELIQGTPVSLSTDVWSVGVLAYVMLSGVSPFLDESLEETCVNICRLDFCFPQEYFSDISQAAKDFIVSTLNQDPRKRPSSASCLQHPWVSAHSGDYSKTPLDTVRLAAFIDRRKQLHDVRPVTNIKGMVSSSMGHTL

Target ORF information:

RefSeq Version	XM_009304450.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kalirin RhoGEF kinase a (kalrna), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304450.3

ORF Insert Sequence:

ATGAATTCTG CTGAAGCGCC GGAGGACGGA CCCAATGATT CGGACACGGA TGCATTCTTC 
AAAACAGGGT CTTTCAGAAG TGAAGGAATT AAAGCCTCTG ATGTCCTTCC TGTGCTGAGA 
GAAAAAATAG CATTTGTGTC AGGCGGTCGT GATAAGCGAG GTGGCCCTAT ACTGACCTTT 
CCTGCAAGGA GTAACCATGA CCGAATAAAA CAAGAGGACC TGAGACGGCT CGTAACATAC 
CTGTCCACTG TGCCCAGTGA GGACGTTTGT AAGAGAGGCT TCACTGTGAT TATTGATATG 
CGTGGCTCTA AATGGGAGCT AATAAAGCCA CTGCTGAAGA CGCTACAGGA AGCATTTCCA 
GCTGAGATCT GCGTCGCTCT CATCATCAAA CCAGACAACT TCTGGCAGAA GCAGAAGACT 
AACTTTGGTA GCGCCAAGTT CACCTTTGAG ACTAGCATGG TATCAGTGGA GGGCTTGACC 
AAACTAGTGG ACCCATCCCA GCTAACGGAC GACTTTGAAG GTTCTCTGGA ATACAATCAT 
GAGGAATGGC TTGAGCTCCG GGTGGCTCTG GAGGAGTTCC TGGCCAGCGC CGTTCACCTC 
CTCTCTCGCC TGGAAGATCT ACAGGAGACT CTGGCTAAGA AGGAGTTCCC GGTGGATGTG 
GAAGCCTCTC GTAGACTCAT CGATGAGCAC ACACAGCTGA AGAAGAAGGT GATGAAGGCT 
CCGGTGGAAG AGCTGGACCA CGAAGGCCAG AGACTCTTGC AGTGCATCCG CTCCAGCGAC 
GGCTTCTCAG GGAGGAACTG CATTTCTGGA AGTGCCGACT TCCAGAGCGT GGTTCCGAAG 
ATCGCCAGTC TGCTGGATAA ACTCCACAGC ACTCGACAGC ACTTGCACCA GATGTGGCAC 
CTACGAAAAC TCAAGCTGGA TCAGTGCTTT CAGCTCCGAC TGTTTGAACA GGACGCAGAG 
AAGATGTTTG ACTGGATCAG TCACAATAAG GAATTGTTTC TTCAGAGCCA CACGGAGATC 
GGTGTGAGCT ACCAGCATGC TGTGGAGCTA CAGACGCAAC ACAACCACTT TGCCATGAAC 
TCAATGAATG CCTATGTGAA CATAAACCGC ATCATGTCGG TGGCGTCGCG GCTGTCGGAG 
GCGGGCCACT ACGCCGCTCA GCAGATCAAA CAGATCAGCA CTCAGCTGGA TCAGGAGTGG 
AAGAGCTTCG CGGCTGCTCT CGATGAACGC AGCACCATCC TTGCCATGTC TGCCGTCTTC 
CATCAGAAAT CTGAACAGTT TTTGTCCGGT GTGGAAACAT GGTGTAAGAT GTGCAGTGAA 
GGCGGCTTGC CATCAGAGAT GCAGGATCTG GAATCGGCCA TTCATCACCA TCAGACGCTC 
TATGAACAAG TCACACAAGC ATACACCGAG GTGAGTCAGG ACGGTAAGGC TTTGCTGGAC 
GTTCTTCAGC GGCCGTTGAG TCCTGGGAAC GCAGAGTCAC TGACTGCGAC AGCGAACTAC 
TCTAAAGCCG TGCACTGTAT ACTGGACGTG GTCCACGAGG TCCTGCATCA TCAGAGACGT 
CTGGAGAACA TCTGGCAGCA CCGTAAAGTC CGTCTGCATC AGAGACTGCA GCTCTGTGTC 
TTCCAGCAGG ACGTCCAACA GGTGATTGAC TGGATAGAGA ATCACGGGGA GGCTTTCCTC 
AGCAAACACA CTGGTGTGGG CAAATCCCTG CACAGAGCCA GAGCCCTCCA GAAACGCCAT 
GATGACTTTG AGGAGGTGGC ACAGAATACA TACACTAATG CAGATAAGCT GCTGGAAGCT 
GCTGAGCAGT TGGCTCAGAC GGGCGAGTGT GACCCTGAGG AGATCTACAA GGCAGCGCGA 
CACCTGGAAG TCAGGATCCA GGACTTTGTT AGGCGAGTGG AGCAAAGAAA ACTACTGCTG 
GACATGTCTG TCTCTTTCCA CACGCACACT AAAGAGTTGT GGACGTGGAT GGAGGACCTG 
CAGAAAGAGC TGCTGGAGGA AGTTTGTTCT GACTCAGTCG ATTCAGTTCA GGAGCTGATA 
AAACAGTTTC AGCAGCAGCA GACGGCCACG CTGGAGGCAA CACTCAATGT CATTAAAGAG 
GGAGAAGATC TTATGCAACA ACTCCGAGAT TCAGCAATGA GCAGTAATAA GATGCCCCAC 
ACCAGCTCCA TCGCTCACAT TGAGGGCGTC CTGCAGCAGC TAGAGGAGGC GCAGGGCCAC 
ATGGAGGAAC TCTTCCATGA GAGAAAGATC AAACTCGACA TCTTCCTGCA ACTGCGCATC 
TTTGAGCAGT ACACCATTGA GGTAACGGCA GAGTTAGACG CGTGGAATGA AGACTTGGTT 
CGTCAGATGA ATGAGTATAC TGGAGAAGAA CCCAGTCTGG CTGAGCAGAG ACTCCAGCGG 
CACGCAGAGA GGAAACTGGC CATGACTAAC ATGACCTACG AAGTTATGCA GCAGGGTCAG 
GACCTGCATC AGTACATCAT GGAAGTCCAA GCTTCAGGTA TTGAGTTGAC CGGAGAGAAG 
GAGGTGGATC TGGCAACACA AGTTCAGGAG CTGCTGGAGT TCCTTCATGA GAAACAGCAC 
GAGTTGGACA TGAGCGCCGA ACACACACAG AAGCGCCTGG AGCAGTGTGT GCAGCTCCGC 
CACCTGCAGG CTGAGGTTAA ACAGGTTCTG GGGTGGATTC GTAATGGTGA ATCTATGTTG 
GATGCCAGTA TGGTGAATGC AAGTTCACTG TCAGAGGCCG AACAACTACA GAGAGAACAT 
GAACAGTTCC AGCTGGCCAT AGAGTCTTTG TTCCACGCTA ACTCTCTCCA GAAAACCCAT 
CAGAGTGCAC TACAGGTCCA GCAGAAGGCA GAAATCATGT TACAGGCTGG ACACTTTGAC 
CCCGAGGCCA TCCGCAGCTG TGCAGAGAAG GTGGCCGTGC ACTGGCAGCA GCTCATGCTC 
AAGATGGAGG ACAGACTCAA ACTGGTTAAT GCCTCGGTGG CCTTCTACAA GACGTCAGAA 
CAGGTGTGTA GTGTCCTGGA GAGTCTGGAG CAGGAGTACC GCAGAGATGA AGACTGGTGT 
GGAGCTCATG ATAAACTAGG ATCTTCATCA GAGACTGATC ACGTTCTGCC TCTCATCAGC 
AAACACCTGG AGCAGAAAGA AGCTTTTCTC AAGGCATGCA CTCTGGCCAG GCGCAATGCT 
GAGGTTTTTC TGAAATACAT CCACCGGAAC AATGTCAGCT CTCAGGGATC ATCTAGCAAC 
ACTCGTGGCC CTGAGCAACA GGTCAAAGCC ATCCTGAATG AACTGCTGCA GAGGGAAAAC 
AGAGTTTTGC ATTTCTGGAC CATGAAGAAG CGGCGTTTGG ATCAGTGTCA GCAGTATGTG 
GTGTTTGAAC GCAATGCCAA ACAGGCATTA GAGTGGATTG AGGAGGCAGG AGAGGTCTAT 
CTGGCCACTC ACACCTGCAT CGGAGACTCT ATGGAGAAAA CTCAGGGACT CCTCAAAGAA 
TACGAAGAAT TCTGCATCTC CGCAAAGCAA ACTAAGGAGA AAGTGAAGAT GCTGATCCAG 
CTGGCGGATA ACTTTGTTGA GAAAGGACAC ATGCATGTCA CCGACTTGAA GAAGTGTCTG 
ACGGCAGTGG ACAAGCGCTA CAGGGACTTC TCCATGCGCA TGGGCAAATA CCGCTGTGAC 
CTGGAAAATG TGCTGGGCAT CAGCTCTGAG GATAATAAGG ATCTGGAGCT GGATATCATC 
CCAGCCAGTC TGACTGGCCC AGAGGTTAAA CTCCGTGACC CCAACCACGA GGTGAATGAA 
GAAAAGAGGA AATCAGCCCG GAAGAAAGAG TTTATTATAG CAGAGCTGTT GCAGACAGAA 
AAGGCTTATG TGAGGGACTT ACATGAATGC TTAGAGACAT ATTTGTGGGA GATGACCAAT 
GGTGTGGAGG AAATTCCTCC AGGAATCGCC AACAAAGAGC ACATCATCTT TGGCAACATT 
CAGGAGATTT ACGACTTCCA CAATAATATA TTCCTGAAGG AGCTGATCAA TTATGAGCAA 
CTGCCTGAGG ATGTGGGACA TTGCTTTGTG ACATGGGCCG ATAAGTTTAA CATTTATGTG 
ACATACTGCA AAAACAAACC AGATTCCAGT CAGCTCATCC TAGACCATGC TGGGAGTTTT 
TTTGATGAGA TTCAGAAGAG ACACAGTCTG ACCAACTCTA TATCATCATA CCTCATCAAA 
CCTGTACAGA GGATCACCAA ATATCAGCTG CTGCTCAAGG AGCTGCTGTC CTGCTGTGAA 
GAAGGCAAAG GTGAAATTAA AGATGGCCTG GAGGTCATGC TGAGTGTGCC AAAGAGAGCC 
AATGACGCCA TGCACGTCAG CATGCTGGAG GGTTTTGATG CAAATTTGGA TGTTCAGGGT 
GAACTCATCC TCCAAGACAC CTTCCAGGTG TGGGACCCCA AATCACTAAT CCGGAAGGGG 
CGAGACCGCC ACCTCTTTCT CTTCGAGATC TCCCTGGTCT TCAGTAAAGA GATCAAAGAC 
TCAGCAGGGC GATCCAAATA CATCTTTAAA AACAAACTAC TGACTTCTGA ATTGGGGGTG 
ACAGAGCATG TAGAGGGGGA CCCGTGCAAA TTTGCTCTGT GGGTGGGACG CACACCATCG 
TCTGACAACA AGACTGTCCT CAAGGCATCT AGTATTGAGG TGAAGCAAGA ATGGATTAAA 
AACATCCGAG AGGTCATTCA GGAGAGAATA ATTCACCTGA AGGGGGCGCT AAAGGAACCT 
ATCCACATTC CCAAAACTCC AGCCAAACCT CGCAATAACA GCAAGAGGGA CGCTGGTGAG 
GATGCTGACA GCCAGGGAGA CGGCAGCAGT CAGCCGGACA CCATCTCCAT AGCATCCAGA 
ACCTCTCAGA ACACTGTAGA CAGTGACAAG CTCTCTGGAG GGTGTGAGCT GACGGTGGTC 
CTGCAGGACT TTACAGCAAG CAGCTCATCA GAGCTCAGCA TCCAGACGGG TCAGACAGTG 
GAGCTGCTGG AGCGGCCCAG TGACCGTCCA GGATGGTGTC TGGTACGGAC CACCGAAAAG 
ACGCCTCCTC AAGAGGGACT GGTGCCCAGC AGTGCTCTGT GTATCTCGCA CTCACGCAGC 
AGTGTGGAGA TGGACTGCTT TTTCCCAGCA GGCAAAGAAA ACTATACAGT TAGTGGCCCA 
GAAGGAAAGA CAGAGTCAGT GGCCACACTG CAGCCACAGC CATCCCTGAA CTCTCTGCAG 
AGTAGCTCTC CTGGACCCAA GCGCTCTGGG AATACACTGA AGAAATGGCT GACCAGTCCT 
GTCCGACGTC TAAGTCATGG CAGTAACATC AAGAAGATCC CAAGTAAACA GAAACAGAAA 
CGTGATGGTC GCAAGAGTAT TGATATGGGG CCGCCCGAAC TACAGGATGA CACCTTGGAA 
GAGCGAGTGC AAAAAGAAGA GGGCACACTC TCCAAGTCTT CGTCTGGGAT GCACAGTGGA 
GGAGAGGAAG AGCCTGAGGA CGAGTCTCAC ACTCCTCTAC CTCCACCAAT GGAGATCATT 
AAAGACCCGT CGGCTCAGGA AGAGAAGTCG TCTGTGCTTC TGGCTTCCCG GCAGAGCTCG 
GCTGATGTCC CCAGTGCTGC TGAGCTTGTC AGTGCTATAG AAAAACTGGT CAAAACTAAA 
ATGACACTGG AGCCAGGCTC ATACCCGGGA TTCAGCTCAG TCAATCCAGC AGAGCAGAGC 
CCGATTCCAC CCAGAAACCC AGAACTAGAG CAGAAAGCCA AAGCGCTACG AGGACGAATG 
TTTGTAGTCA AGGAATTAAT CCAAACGGAA AAAGACTATG TTAAAGATCT GGGAATTGTG 
GTTGAGGGGT TTATGAAGAG AATCGAGGAG AAAGGAGTTC CTGATAACAT GAAAGGAAAA 
GATAAGATCG TCTTCGGAAA CATTCACCAA ATCTATGACT GGCACAAAGA TTTTTTTGTT 
GGAGAACTGG AGAAATGCCT GGAAGATCAC GAAAAACTGC CTGAACTGTT CTGTAAACAT 
GAGAGACGGT TACACATGTA TGTAGTTTAC TGTCAGAACA AACCCAAATC CGAGTTCATC 
GTTGCAGAAT ATGATTCATA CTTTGAGGGG ATTCAGCAAG ACATTAACTC CAGACTGAGC 
ATCAGCGATT TTCTCATTAA ACCCATTCAG AGAATTACTA AATACCAGCT TTTATTAAAG 
GATGTTTTGA AGTACAGTAC AAAAGCAGGA CTGGATTGTC AGGATATAGA GAATGCAGTT 
GACCTGATGT CCCAGGTTCC CAAGCTTTGC AATGACATGA TGAATCTGGG AAGACTGCAG 
GGATATGAGG GGAAGTTGAC GAGTCAAGGA AAACTCTTGC AGCAGGAGAC GTTCTTTGTG 
ACCGAGCAGG ACTCTGGCGT TCTGTCCCGC AGTAAAGAGC GCAGGGTTTT CCTGTTCGAA 
CAAATTGTCA TCTTCAGCGA GCTGTTACGT AAAGGCTCTT CCACTCCAGG GTACCAGTTC 
AAGAAGAGCA TAAAGGTGAG CTTCCTTGAC TTGGAAGAGC ATGTTGAAAA TGATCCATGC 
AAGTTTGTGC TGTCGTGTCG TGGGTCGGCA GAACGTTTTA CCCTTCAAGC AGCAAACACG 
GACATTAAAC AAGTCTGGGT TCAGCATATC ACCCAACTAC TAGATTTACA AAGCAACTTC 
TTGTCAGCTC TGCAGTCTCC TATTGAGTAT CAGAAGGAGC GTAGTGGCTC TCAATCTCTG 
ACCCGGAACT CTTCTGGGCG GATGGGTCAG TCCAACAGCA GGCCCTGCTC CACGGTTTCA 
CTCGGAGCAG AGAAACCCTC TCTGTCTGGA CGGAGCTCCA CCCCGCTAAA GCTATCTACC 
TCCAATGGTG GTCCATGCTC TGAAAGATAC CGCAGGTTTG AAGGCTTGGG CTGCAACGGT 
ACATCGTCCT CCTTGATGGT GACTCAAGAT TATACTGCAC TGAAAGAGAA TGAGATCTGT 
GTCACACAGG GTGAGGCCGT GCAGGTACTG GCCACTAACC AGCAGAACAT GTACCTTGTG 
TACCGACCCG CAAATAGCCA ATCACCACCT GCAGAGGGCT GGGTGCCGGG ACACATCCTA 
GGCCCCGTCA CAAAACCTCT CATAGACACT ACTGCAGACG CAAGCATCAA GAAATCTCTT 
TCGTGGAACA CACTTCGTGC TCGTAAGCGT GCAGAAAAGG ACAGCACGCC CCTCAAAAGT 
GACTTTAAAG TTGAGAATGG ACTGCGTAAG CCAAAGGAAA TTCTCAGCAG CAAAGTCAAA 
GTGAAAGAGT CCCGCAGTTC TGATGAGTCT GAATGTGAGG ATGAACTAGA CCCCAAAACT 
AGTATGGAGT TACTGAACCC TAATTTCATC CAAGAGGTCG CTCCAGAGTT TCTGACGCCT 
CTAACAGACG TGACGTGTGC TCTGGGAGAG ACGGTGGTTC TGTGCTGTAA AGTGTGTGCT 
CGACCCAAAC CCACAATCAC CTTGAAAGGA CCTGATCAAA GTCTGCTGGC CAACAATAAC 
CGCTTCACTA TTAATATCAG AGAAACCGGT GATATAGTGT TGAAAATCTG CAATCTAATG 
CCGCAGGACA CTGGCATCTA CACGTGTGTT GCTGTGAATG ACCATGGTAC AGCCTCCTCA 
TCTGCATCCA TTAAAGTACA AGGTATCCCA GCAGCCCCTG CTCGACCGGT GGCACAGGAG 
GCAAGCAGCA CTGCAGTGAT TGTCCACTGG CTTCCTCCAG CCAGCTCAGC AAATTCTGCC 
ATCTCCAGCT ACACTGTGGA ATATAGGCAG GAAGATTCTC TGGTATGGCA GCAGTTGGTG 
GTCTCCACGG CAGATGTGTT TGTGAAGATT GACAATCTGA TTCCTGGTGG GCACTACCAG 
TTCAGAGTCA GTGCCAGTAA TCCCTGGGGC ACCAGCCCGC CTAGTGAACC CTCAAACATG 
GTGACATTAT CCAGCACAGC ATCAACATAT GATGGCGCTG GAATCCAGTG GAAAGACAAC 
TTTGAATCTG CTTTTACAGA ATTATGTGAA ATTGGGCGGG GTCGTTTCTC TGTGGTGAGG 
AAGTGTCTCA GTAAAGCCAC TAAGCAGGAG GTTGCTGTGA AGTTTGTGAA TAAGAAGATG 
CACAAGAAAG AACAGGTGGC CCATGAAGCT GATATTCTAC GCCATGTCCA GCACCCCCAA 
CTGGTGTCAC TGATTGATAC ATACGAGTCG CCTACAGCCT ACATGCTGAT TCTTGAACTT 
GTTGAGGATG GACGTCTTCT TGACTATCTG GTAGCTCATG ATGAGCTGAT GGAGGAGAAA 
GTGGCATTCT TTATTAAAGA TACACTGGAA GCTCTGCAGC ACTTGCACAC CTGCAGAGTG 
GCTCATCTAG ACCTCAAGCC TGAAAACTTG TTGGTGGACC TCCACGTTCC GGTACCCTGC 
GTGAAGCTCT CAGATCTAGG TGATGCTGTA CAGGTTTCAA GTCACCGCTA CGTACATCTC 
CTCCTGGGTA ACCCCGAGTT CGCTGCTCCA GAGCTCATCC AGGGCACTCC AGTGTCCCTC 
TCTACAGATG TGTGGAGTGT AGGGGTGCTG GCCTACGTCA TGCTTAGTGG AGTCTCCCCA 
TTCCTGGATG AGAGTCTGGA GGAGACCTGC GTCAACATCT GCAGGCTGGA CTTCTGCTTT 
CCTCAAGAGT ACTTCTCCGA TATAAGCCAG GCAGCCAAAG ACTTCATTGT GTCCACGCTT 
AACCAGGATC CCAGGAAGAG GCCAAGCTCG GCCTCGTGTC TTCAGCACCC ATGGGTCAGT 
GCTCATAGTG GAGATTACTC CAAAACACCT CTGGATACTG TCAGACTCGC TGCATTTATA 
GACAGACGCA AACAGCTACA TGATGTCAGG CCTGTCACAA ACATTAAAGG AATGGTCAGC 
AGCAGTATGG GACACACCTT ATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kalrna ( XM_009304451.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302726.2 Danio rerio kalirin RhoGEF kinase a (kalrna), transcript variant X4, mRNA.

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CloneID	ODa60289
Clone ID Related Accession (Same CDS sequence)	XM_009304451.3 , XM_009304451.2
Accession Version	XM_009304451.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	kalirin isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009304451.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTG CTGAAGCGCC GGAGGACGGA CCCAATGATT CGGACACGGA TGCATTCTTC 
AAAACAGGGT CTTTCAGAAG TGAAGGAATT AAAGCCTCTG ATGTCCTTCC TGTGCTGAGA 
GAAAAAATAG CATTTGTGTC AGGCGGTCGT GATAAGCGAG GTGGCCCTAT ACTGACCTTT 
CCTGCAAGGA GTAACCATGA CCGAATAAAA CAAGAGGACC TGAGACGGCT CGTAACATAC 
CTGTCCACTG TGCCCAGTGA GGACGTTTGT AAGAGAGGCT TCACTGTGAT TATTGATATG 
CGTGGCTCTA AATGGGAGCT AATAAAGCCA CTGCTGAAGA CGCTACAGGA AGCATTTCCA 
GCTGAGATCT GCGTCGCTCT CATCATCAAA CCAGACAACT TCTGGCAGAA GCAGAAGACT 
AACTTTGGTA GCGCCAAGTT CACCTTTGAG ACTAGCATGG TATCAGTGGA GGGCTTGACC 
AAACTAGTGG ACCCATCCCA GCTAACGGAC GACTTTGAAG GTTCTCTGGA ATACAATCAT 
GAGGAATGGC TTGAGCTCCG GGTGGCTCTG GAGGAGTTCC TGGCCAGCGC CGTTCACCTC 
CTCTCTCGCC TGGAAGATCT ACAGGAGACT CTGGCTAAGA AGGAGTTCCC GGTGGATGTG 
GAAGCCTCTC GTAGACTCAT CGATGAGCAC ACACAGCTGA AGAAGAAGGT GATGAAGGCT 
CCGGTGGAAG AGCTGGACCA CGAAGGCCAG AGACTCTTGC AGTGCATCCG CTCCAGCGAC 
GGCTTCTCAG GGAGGAACTG CATTTCTGGA AGTGCCGACT TCCAGAGCGT GGTTCCGAAG 
ATCGCCAGTC TGCTGGATAA ACTCCACAGC ACTCGACAGC ACTTGCACCA GATGTGGCAC 
CTACGAAAAC TCAAGCTGGA TCAGTGCTTT CAGCTCCGAC TGTTTGAACA GGACGCAGAG 
AAGATGTTTG ACTGGATCAG TCACAATAAG GAATTGTTTC TTCAGAGCCA CACGGAGATC 
GGTGTGAGCT ACCAGCATGC TGTGGAGCTA CAGACGCAAC ACAACCACTT TGCCATGAAC 
TCAATGAATG CCTATGTGAA CATAAACCGC ATCATGTCGG TGGCGTCGCG GCTGTCGGAG 
GCGGGCCACT ACGCCGCTCA GCAGATCAAA CAGATCAGCA CTCAGCTGGA TCAGGAGTGG 
AAGAGCTTCG CGGCTGCTCT CGATGAACGC AGCACCATCC TTGCCATGTC TGCCGTCTTC 
CATCAGAAAT CTGAACAGTT TTTGTCCGGT GTGGAAACAT GGTGTAAGAT GTGCAGTGAA 
GGCGGCTTGC CATCAGAGAT GCAGGATCTG GAATCGGCCA TTCATCACCA TCAGACGCTC 
TATGAACAAG TCACACAAGC ATACACCGAG GTGAGTCAGG ACGGTAAGGC TTTGCTGGAC 
GTTCTTCAGC GGCCGTTGAG TCCTGGGAAC GCAGAGTCAC TGACTGCGAC AGCGAACTAC 
TCTAAAGCCG TGCACTGTAT ACTGGACGTG GTCCACGAGG TCCTGCATCA TCAGAGACGT 
CTGGAGAACA TCTGGCAGCA CCGTAAAGTC CGTCTGCATC AGAGACTGCA GCTCTGTGTC 
TTCCAGCAGG ACGTCCAACA GGTGATTGAC TGGATAGAGA ATCACGGGGA GGCTTTCCTC 
AGCAAACACA CTGGTGTGGG CAAATCCCTG CACAGAGCCA GAGCCCTCCA GAAACGCCAT 
GATGACTTTG AGGAGGTGGC ACAGAATACA TACACTAATG CAGATAAGCT GCTGGAAGCT 
GCTGAGCAGT TGGCTCAGAC GGGCGAGTGT GACCCTGAGG AGATCTACAA GGCAGCGCGA 
CACCTGGAAG TCAGGATCCA GGACTTTGTT AGGCGAGTGG AGCAAAGAAA ACTACTGCTG 
GACATGTCTG TCTCTTTCCA CACGCACACT AAAGAGTTGT GGACGTGGAT GGAGGACCTG 
CAGAAAGAGC TGCTGGAGGA AGTTTGTTCT GACTCAGTCG ATTCAGTTCA GGAGCTGATA 
AAACAGTTTC AGCAGCAGCA GACGGCCACG CTGGAGGCAA CACTCAATGT CATTAAAGAG 
GGAGAAGATC TTATGCAACA ACTCCGAGAT TCAGCAATGA GCAGTAATAA GATGCCCCAC 
ACCAGCTCCA TCGCTCACAT TGAGGGCGTC CTGCAGCAGC TAGAGGAGGC GCAGGGCCAC 
ATGGAGGAAC TCTTCCATGA GAGAAAGATC AAACTCGACA TCTTCCTGCA ACTGCGCATC 
TTTGAGCAGT ACACCATTGA GGTAACGGCA GAGTTAGACG CGTGGAATGA AGACTTGGTT 
CGTCAGATGA ATGAGTATAC TGGAGAAGAA CCCAGTCTGG CTGAGCAGAG ACTCCAGCGG 
CACGCAGAGA GGAAACTGGC CATGACTAAC ATGACCTACG AAGTTATGCA GCAGGGTCAG 
GACCTGCATC AGTACATCAT GGAAGTCCAA GCTTCAGGTA TTGAGTTGAC CGGAGAGAAG 
GAGGTGGATC TGGCAACACA AGTTCAGGAG CTGCTGGAGT TCCTTCATGA GAAACAGCAC 
GAGTTGGACA TGAGCGCCGA ACACACACAG AAGCGCCTGG AGCAGTGTGT GCAGCTCCGC 
CACCTGCAGG CTGAGGTTAA ACAGGTTCTG GGGTGGATTC GTAATGGTGA ATCTATGTTG 
GATGCCAGTA TGGTGAATGC AAGTTCACTG TCAGAGGCCG AACAACTACA GAGAGAACAT 
GAACAGTTCC AGCTGGCCAT AGAGTCTTTG TTCCACGCTA ACTCTCTCCA GAAAACCCAT 
CAGAGTGCAC TACAGGTCCA GCAGAAGGCA GAAATCATGT TACAGGCTGG ACACTTTGAC 
CCCGAGGCCA TCCGCAGCTG TGCAGAGAAG GTGGCCGTGC ACTGGCAGCA GCTCATGCTC 
AAGATGGAGG ACAGACTCAA ACTGGTTAAT GCCTCGGTGG CCTTCTACAA GACGTCAGAA 
CAGGTGTGTA GTGTCCTGGA GAGTCTGGAG CAGGAGTACC GCAGAGATGA AGACTGGTGT 
GGAGCTCATG ATAAACTAGG ATCTTCATCA GAGACTGATC ACGTTCTGCC TCTCATCAGC 
AAACACCTGG AGCAGAAAGA AGCTTTTCTC AAGGCATGCA CTCTGGCCAG GCGCAATGCT 
GAGGTTTTTC TGAAATACAT CCACCGGAAC AATGTCAGCT CTCAGGGATC ATCTAGCAAC 
ACTCGTGGCC CTGAGCAACA GGTCAAAGCC ATCCTGAATG AACTGCTGCA GAGGGAAAAC 
AGAGTTTTGC ATTTCTGGAC CATGAAGAAG CGGCGTTTGG ATCAGTGTCA GCAGTATGTG 
GTGTTTGAAC GCAATGCCAA ACAGGCATTA GAGTGGATTG AGGAGGCAGG AGAGGTCTAT 
CTGGCCACTC ACACCTGCAT CGGAGACTCT ATGGAGAAAA CTCAGGGACT CCTCAAAGAA 
TACGAAGAAT TCTGCATCTC CGCAAAGCAA ACTAAGGAGA AAGTGAAGAT GCTGATCCAG 
CTGGCGGATA ACTTTGTTGA GAAAGGACAC ATGCATGTCA CCGACTTGAA GAAGTGTCTG 
ACGGCAGTGG ACAAGCGCTA CAGGGACTTC TCCATGCGCA TGGGCAAATA CCGCTGTGAC 
CTGGAAAATG TGCTGGGCAT CAGCTCTGAG GATAATAAGG ATCTGGAGCT GGATATCATC 
CCAGCCAGTC TGACTGGCCC AGAGGTTAAA CTCCGTGACC CCAACCACGA GGTGAATGAA 
GAAAAGAGGA AATCAGCCCG GAAGAAAGAG TTTATTATAG CAGAGCTGTT GCAGACAGAA 
AAGGCTTATG TGAGGGACTT ACATGAATGC TTAGAGACAT ATTTGTGGGA GATGACCAAT 
GGTGTGGAGG AAATTCCTCC AGGAATCGCC AACAAAGAGC ACATCATCTT TGGCAACATT 
CAGGAGATTT ACGACTTCCA CAATAATATA TTCCTGAAGG AGCTGATCAA TTATGAGCAA 
CTGCCTGAGG ATGTGGGACA TTGCTTTGTG ACATGGGCCG ATAAGTTTAA CATTTATGTG 
ACATACTGCA AAAACAAACC AGATTCCAGT CAGCTCATCC TAGACCATGC TGGGAGTTTT 
TTTGATGAGA TTCAGAAGAG ACACAGTCTG ACCAACTCTA TATCATCATA CCTCATCAAA 
CCTGTACAGA GGATCACCAA ATATCAGCTG CTGCTCAAGG AGCTGCTGTC CTGCTGTGAA 
GAAGGCAAAG GTGAAATTAA AGATGGCCTG GAGGTCATGC TGAGTGTGCC AAAGAGAGCC 
AATGACGCCA TGCACGTCAG CATGCTGGAG GGTTTTGATG CAAATTTGGA TGTTCAGGGT 
GAACTCATCC TCCAAGACAC CTTCCAGGTG TGGGACCCCA AATCACTAAT CCGGAAGGGG 
CGAGACCGCC ACCTCTTTCT CTTCGAGATC TCCCTGGTCT TCAGTAAAGA GATCAAAGAC 
TCAGCAGGGC GATCCAAATA CATCTTTAAA AACAAACTAC TGACTTCTGA ATTGGGGGTG 
ACAGAGCATG TAGAGGGGGA CCCGTGCAAA TTTGCTCTGT GGGTGGGACG CACACCATCG 
TCTGACAACA AGACTGTCCT CAAGGCATCT AGTATTGAGG TGAAGCAAGA ATGGATTAAA 
AACATCCGAG AGGTCATTCA GGAGAGAATA ATTCACCTGA AGGGGGCGCT AAAGGAACCT 
ATCCACATTC CCAAAACTCC AGCCAAACCT CGCAATAACA GCAAGAGGGA CGCTGGTGAG 
GATGCTGACA GCCAGGGAGA CGGCAGCAGT CAGCCGGACA CCATCTCCAT AGCATCCAGA 
ACCTCTCAGA ACACTGTAGA CAGTGACAAG CTCTCTGGAG GGTGTGAGCT GACGGTGGTC 
CTGCAGGACT TTACAGCAAG CAGCTCATCA GAGCTCAGCA TCCAGACGGG TCAGACAGTG 
GAGCTGCTGG AGCGGCCCAG TGACCGTCCA GGATGGTGTC TGGTACGGAC CACCGAAAAG 
ACGCCTCCTC AAGAGGGACT GGTGCCCAGC AGTGCTCTGT GTATCTCGCA CTCACGCAGC 
AGTGTGGAGA TGGACTGCTT TTTCCCAGCA GGCAAAGAAA ACTATACAGT TAGTGGCCCA 
GAAGGAAAGA CAGAGTCAGT GGCCACACTG CAGCCACAGC CATCCCTGAA CTCTCTGCAG 
AGTAGCTCTC CTGGACCCAA GCGCTCTGGG AATACACTGA AGAAATGGCT GACCAGTCCT 
GTCCGACGTC TAAGTCATGG CAGTAACATC AAGAAGATCC CAAGTAAACA GAAACAGAAA 
CGTGATGGTC GCAAGAGTAT TGATATGGGG CCGCCCGAAC TACAGGATGA CACCTTGGAA 
GAGCGAGTGC AAAAAGAAGA GGGCACACTC TCCAAGTCTT CGTCTGGGAT GCACAGTGGA 
GGAGAGGAAG AGCCTGAGGA CGAGTCTCAC ACTCCTCTAC CTCCACCAAT GGAGATCATT 
AAAGACCCGT CGGCTCAGGA AGAGAAGACA CTGGAGCCAG GCTCATACCC GGGATTCAGC 
TCAGTCAATC CAGCAGAGCA GAGCCCGATT CCACCCAGAA ACCCAGAACT AGAGCAGAAA 
GCCAAAGCGC TACGAGGACG AATGTTTGTA GTCAAGGAAT TAATCCAAAC GGAAAAAGAC 
TATGTTAAAG ATCTGGGAAT TGTGGTTGAG GGGTTTATGA AGAGAATCGA GGAGAAAGGA 
GTTCCTGATA ACATGAAAGG AAAAGATAAG ATCGTCTTCG GAAACATTCA CCAAATCTAT 
GACTGGCACA AAGATTTTTT TGTTGGAGAA CTGGAGAAAT GCCTGGAAGA TCACGAAAAA 
CTGCCTGAAC TGTTCTGTAA ACATGAGAGA CGGTTACACA TGTATGTAGT TTACTGTCAG 
AACAAACCCA AATCCGAGTT CATCGTTGCA GAATATGATT CATACTTTGA GGGGATTCAG 
CAAGACATTA ACTCCAGACT GAGCATCAGC GATTTTCTCA TTAAACCCAT TCAGAGAATT 
ACTAAATACC AGCTTTTATT AAAGGATGTT TTGAAGTACA GTACAAAAGC AGGACTGGAT 
TGTCAGGATA TAGAGAATGC AGTTGACCTG ATGTCCCAGG TTCCCAAGCT TTGCAATGAC 
ATGATGAATC TGGGAAGACT GCAGGGATAT GAGGGGAAGT TGACGAGTCA AGGAAAACTC 
TTGCAGCAGG AGACGTTCTT TGTGACCGAG CAGGACTCTG GCGTTCTGTC CCGCAGTAAA 
GAGCGCAGGG TTTTCCTGTT CGAACAAATT GTCATCTTCA GCGAGCTGTT ACGTAAAGGC 
TCTTCCACTC CAGGGTACCA GTTCAAGAAG AGCATAAAGG TGAGCTTCCT TGACTTGGAA 
GAGCATGTTG AAAATGATCC ATGCAAGTTT GTGCTGTCGT GTCGTGGGTC GGCAGAACGT 
TTTACCCTTC AAGCAGCAAA CACGGACATT AAACAAGTCT GGGTTCAGCA TATCACCCAA 
CTACTAGATT TACAAAGCAA CTTCTTGTCA GCTCTGCAGT CTCCTATTGA GTATCAGAAG 
GAGCGTAGTG GCTCTCAATC TCTGACCCGG AACTCTTCTG GGCGGATGGG TCAGTCCAAC 
AGCAGGCCCT GCTCCACGGT TTCACTCGGA GCAGAGAAAC CCTCTCTGTC TGGACGGAGC 
TCCACCCCGC TAAAGCTATC TACCTCCAAT GGTGGTCCAT GCTCTGAAAG ATACCGCAGG 
CAGTTTGAAG GCTTGGGCTG CAACGGTACA TCGTCCTCCT TGATGGTGAC TCAAGATTAT 
ACTGCACTGA AAGAGAATGA GATCTGTGTC ACACAGGGTG AGGCCGTGCA GGTACTGGCC 
ACTAACCAGC AGAACATGTA CCTTGTGTAC CGACCCGCAA ATAGCCAATC ACCACCTGCA 
GAGGGCTGGG TGCCGGGACA CATCCTAGGC CCCGTCACAA AACCTCTCAT AGACACTACT 
GCAGACGCAA GCATCAAGAA ATCTCTTTCG TGGAACACAC TTCGTGCTCG TAAGCGTGCA 
GAAAAGGACA GCACGCCCCT CAAAAGTGAC TTTAAAGTTG AGAATGGACT GCGTAAGCCA 
AAGGAAATTC TCAGCAGCAA AGTCAAAGTG AAAGAGTCCC GCAGTTCTGA TGAGTCTGAA 
TGTGAGGATG AACTAGACCC CAAAACTAGT ATGGAGTTAC TGAACCCTAA TTTCATCCAA 
GAGGTCGCTC CAGAGTTTCT GACGCCTCTA ACAGACGTGA CGTGTGCTCT GGGAGAGACG 
GTGGTTCTGT GCTGTAAAGT GTGTGCTCGA CCCAAACCCA CAATCACCTT GAAAGGACCT 
GATCAAAGTC TGCTGGCCAA CAATAACCGC TTCACTATTA ATATCAGAGA AACCGGTGAT 
ATAGTGTTGA AAATCTGCAA TCTAATGCCG CAGGACACTG GCATCTACAC GTGTGTTGCT 
GTGAATGACC ATGGTACAGC CTCCTCATCT GCATCCATTA AAGTACAAGG TATCCCAGCA 
GCCCCTGCTC GACCGGTGGC ACAGGAGGCA AGCAGCACTG CAGTGATTGT CCACTGGCTT 
CCTCCAGCCA GCTCAGCAAA TTCTGCCATC TCCAGCTACA CTGTGGAATA TAGGCAGGAA 
GATTCTCTGG TATGGCAGCA GTTGGTGGTC TCCACGGCAG ATGTGTTTGT GAAGATTGAC 
AATCTGATTC CTGGTGGGCA CTACCAGTTC AGAGTCAGTG CCAGTAATCC CTGGGGCACC 
AGCCCGCCTA GTGAACCCTC AAACATGGTG ACATTATCCA GCACAGCATC AACATATGAT 
GGCGCTGGAA TCCAGTGGAA AGACAACTTT GAATCTGCTT TTACAGAATT ATGTGAAATT 
GGGCGGGGTC GTTTCTCTGT GGTGAGGAAG TGTCTCAGTA AAGCCACTAA GCAGGAGGTT 
GCTGTGAAGT TTGTGAATAA GAAGATGCAC AAGAAAGAAC AGGTGGCCCA TGAAGCTGAT 
ATTCTACGCC ATGTCCAGCA CCCCCAACTG GTGTCACTGA TTGATACATA CGAGTCGCCT 
ACAGCCTACA TGCTGATTCT TGAACTTGTT GAGGATGGAC GTCTTCTTGA CTATCTGGTA 
GCTCATGATG AGCTGATGGA GGAGAAAGTG GCATTCTTTA TTAAAGATAC ACTGGAAGCT 
CTGCAGCACT TGCACACCTG CAGAGTGGCT CATCTAGACC TCAAGCCTGA AAACTTGTTG 
GTGGACCTCC ACGTTCCGGT ACCCTGCGTG AAGCTCTCAG ATCTAGGTGA TGCTGTACAG 
GTTTCAAGTC ACCGCTACGT ACATCTCCTC CTGGGTAACC CCGAGTTCGC TGCTCCAGAG 
CTCATCCAGG GCACTCCAGT GTCCCTCTCT ACAGATGTGT GGAGTGTAGG GGTGCTGGCC 
TACGTCATGC TTAGTGGAGT CTCCCCATTC CTGGATGAGA GTCTGGAGGA GACCTGCGTC 
AACATCTGCA GGCTGGACTT CTGCTTTCCT CAAGAGTACT TCTCCGATAT AAGCCAGGCA 
GCCAAAGACT TCATTGTGTC CACGCTTAAC CAGGATCCCA GGAAGAGGCC AAGCTCGGCC 
TCGTGTCTTC AGCACCCATG GGTCAGTGCT CATAGTGGAG ATTACTCCAA AACACCTCTG 
GATACTGTCA GACTCGCTGC ATTTATAGAC AGACGCAAAC AGCTACATGA TGTCAGGCCT 
GTCACAAACA TTAAAGGAAT GGTCAGCAGC AGTATGGGAC ACACCTTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302726.2
CDS	551..9361
Translation	MNSAEAPEDGPNDSDTDAFFKTGSFRSEGIKASDVLPVLREKIAFVSGGRDKRGGPILTFPARSNHDRIKQEDLRRLVTYLSTVPSEDVCKRGFTVIIDMRGSKWELIKPLLKTLQEAFPAEICVALIIKPDNFWQKQKTNFGSAKFTFETSMVSVEGLTKLVDPSQLTDDFEGSLEYNHEEWLELRVALEEFLASAVHLLSRLEDLQETLAKKEFPVDVEASRRLIDEHTQLKKKVMKAPVEELDHEGQRLLQCIRSSDGFSGRNCISGSADFQSVVPKIASLLDKLHSTRQHLHQMWHLRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHAVELQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYAAQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKSEQFLSGVETWCKMCSEGGLPSEMQDLESAIHHHQTLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNAESLTATANYSKAVHCILDVVHEVLHHQRRLENIWQHRKVRLHQRLQLCVFQQDVQQVIDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKELLEEVCSDSVDSVQELIKQFQQQQTATLEATLNVIKEGEDLMQQLRDSAMSSNKMPHTSSIAHIEGVLQQLEEAQGHMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLVRQMNEYTGEEPSLAEQRLQRHAERKLAMTNMTYEVMQQGQDLHQYIMEVQASGIELTGEKEVDLATQVQELLEFLHEKQHELDMSAEHTQKRLEQCVQLRHLQAEVKQVLGWIRNGESMLDASMVNASSLSEAEQLQREHEQFQLAIESLFHANSLQKTHQSALQVQQKAEIMLQAGHFDPEAIRSCAEKVAVHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGAHDKLGSSSETDHVLPLISKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSSQGSSSNTRGPEQQVKAILNELLQRENRVLHFWTMKKRRLDQCQQYVVFERNAKQALEWIEEAGEVYLATHTCIGDSMEKTQGLLKEYEEFCISAKQTKEKVKMLIQLADNFVEKGHMHVTDLKKCLTAVDKRYRDFSMRMGKYRCDLENVLGISSEDNKDLELDIIPASLTGPEVKLRDPNHEVNEEKRKSARKKEFIIAELLQTEKAYVRDLHECLETYLWEMTNGVEEIPPGIANKEHIIFGNIQEIYDFHNNIFLKELINYEQLPEDVGHCFVTWADKFNIYVTYCKNKPDSSQLILDHAGSFFDEIQKRHSLTNSISSYLIKPVQRITKYQLLLKELLSCCEEGKGEIKDGLEVMLSVPKRANDAMHVSMLEGFDANLDVQGELILQDTFQVWDPKSLIRKGRDRHLFLFEISLVFSKEIKDSAGRSKYIFKNKLLTSELGVTEHVEGDPCKFALWVGRTPSSDNKTVLKASSIEVKQEWIKNIREVIQERIIHLKGALKEPIHIPKTPAKPRNNSKRDAGEDADSQGDGSSQPDTISIASRTSQNTVDSDKLSGGCELTVVLQDFTASSSSELSIQTGQTVELLERPSDRPGWCLVRTTEKTPPQEGLVPSSALCISHSRSSVEMDCFFPAGKENYTVSGPEGKTESVATLQPQPSLNSLQSSSPGPKRSGNTLKKWLTSPVRRLSHGSNIKKIPSKQKQKRDGRKSIDMGPPELQDDTLEERVQKEEGTLSKSSSGMHSGGEEEPEDESHTPLPPPMEIIKDPSAQEEKTLEPGSYPGFSSVNPAEQSPIPPRNPELEQKAKALRGRMFVVKELIQTEKDYVKDLGIVVEGFMKRIEEKGVPDNMKGKDKIVFGNIHQIYDWHKDFFVGELEKCLEDHEKLPELFCKHERRLHMYVVYCQNKPKSEFIVAEYDSYFEGIQQDINSRLSISDFLIKPIQRITKYQLLLKDVLKYSTKAGLDCQDIENAVDLMSQVPKLCNDMMNLGRLQGYEGKLTSQGKLLQQETFFVTEQDSGVLSRSKERRVFLFEQIVIFSELLRKGSSTPGYQFKKSIKVSFLDLEEHVENDPCKFVLSCRGSAERFTLQAANTDIKQVWVQHITQLLDLQSNFLSALQSPIEYQKERSGSQSLTRNSSGRMGQSNSRPCSTVSLGAEKPSLSGRSSTPLKLSTSNGGPCSERYRRQFEGLGCNGTSSSLMVTQDYTALKENEICVTQGEAVQVLATNQQNMYLVYRPANSQSPPAEGWVPGHILGPVTKPLIDTTADASIKKSLSWNTLRARKRAEKDSTPLKSDFKVENGLRKPKEILSSKVKVKESRSSDESECEDELDPKTSMELLNPNFIQEVAPEFLTPLTDVTCALGETVVLCCKVCARPKPTITLKGPDQSLLANNNRFTINIRETGDIVLKICNLMPQDTGIYTCVAVNDHGTASSSASIKVQGIPAAPARPVAQEASSTAVIVHWLPPASSANSAISSYTVEYRQEDSLVWQQLVVSTADVFVKIDNLIPGGHYQFRVSASNPWGTSPPSEPSNMVTLSSTASTYDGAGIQWKDNFESAFTELCEIGRGRFSVVRKCLSKATKQEVAVKFVNKKMHKKEQVAHEADILRHVQHPQLVSLIDTYESPTAYMLILELVEDGRLLDYLVAHDELMEEKVAFFIKDTLEALQHLHTCRVAHLDLKPENLLVDLHVPVPCVKLSDLGDAVQVSSHRYVHLLLGNPEFAAPELIQGTPVSLSTDVWSVGVLAYVMLSGVSPFLDESLEETCVNICRLDFCFPQEYFSDISQAAKDFIVSTLNQDPRKRPSSASCLQHPWVSAHSGDYSKTPLDTVRLAAFIDRRKQLHDVRPVTNIKGMVSSSMGHTL

Target ORF information:

RefSeq Version	XM_009304451.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio kalirin RhoGEF kinase a (kalrna), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304451.3

ORF Insert Sequence:

ATGAATTCTG CTGAAGCGCC GGAGGACGGA CCCAATGATT CGGACACGGA TGCATTCTTC 
AAAACAGGGT CTTTCAGAAG TGAAGGAATT AAAGCCTCTG ATGTCCTTCC TGTGCTGAGA 
GAAAAAATAG CATTTGTGTC AGGCGGTCGT GATAAGCGAG GTGGCCCTAT ACTGACCTTT 
CCTGCAAGGA GTAACCATGA CCGAATAAAA CAAGAGGACC TGAGACGGCT CGTAACATAC 
CTGTCCACTG TGCCCAGTGA GGACGTTTGT AAGAGAGGCT TCACTGTGAT TATTGATATG 
CGTGGCTCTA AATGGGAGCT AATAAAGCCA CTGCTGAAGA CGCTACAGGA AGCATTTCCA 
GCTGAGATCT GCGTCGCTCT CATCATCAAA CCAGACAACT TCTGGCAGAA GCAGAAGACT 
AACTTTGGTA GCGCCAAGTT CACCTTTGAG ACTAGCATGG TATCAGTGGA GGGCTTGACC 
AAACTAGTGG ACCCATCCCA GCTAACGGAC GACTTTGAAG GTTCTCTGGA ATACAATCAT 
GAGGAATGGC TTGAGCTCCG GGTGGCTCTG GAGGAGTTCC TGGCCAGCGC CGTTCACCTC 
CTCTCTCGCC TGGAAGATCT ACAGGAGACT CTGGCTAAGA AGGAGTTCCC GGTGGATGTG 
GAAGCCTCTC GTAGACTCAT CGATGAGCAC ACACAGCTGA AGAAGAAGGT GATGAAGGCT 
CCGGTGGAAG AGCTGGACCA CGAAGGCCAG AGACTCTTGC AGTGCATCCG CTCCAGCGAC 
GGCTTCTCAG GGAGGAACTG CATTTCTGGA AGTGCCGACT TCCAGAGCGT GGTTCCGAAG 
ATCGCCAGTC TGCTGGATAA ACTCCACAGC ACTCGACAGC ACTTGCACCA GATGTGGCAC 
CTACGAAAAC TCAAGCTGGA TCAGTGCTTT CAGCTCCGAC TGTTTGAACA GGACGCAGAG 
AAGATGTTTG ACTGGATCAG TCACAATAAG GAATTGTTTC TTCAGAGCCA CACGGAGATC 
GGTGTGAGCT ACCAGCATGC TGTGGAGCTA CAGACGCAAC ACAACCACTT TGCCATGAAC 
TCAATGAATG CCTATGTGAA CATAAACCGC ATCATGTCGG TGGCGTCGCG GCTGTCGGAG 
GCGGGCCACT ACGCCGCTCA GCAGATCAAA CAGATCAGCA CTCAGCTGGA TCAGGAGTGG 
AAGAGCTTCG CGGCTGCTCT CGATGAACGC AGCACCATCC TTGCCATGTC TGCCGTCTTC 
CATCAGAAAT CTGAACAGTT TTTGTCCGGT GTGGAAACAT GGTGTAAGAT GTGCAGTGAA 
GGCGGCTTGC CATCAGAGAT GCAGGATCTG GAATCGGCCA TTCATCACCA TCAGACGCTC 
TATGAACAAG TCACACAAGC ATACACCGAG GTGAGTCAGG ACGGTAAGGC TTTGCTGGAC 
GTTCTTCAGC GGCCGTTGAG TCCTGGGAAC GCAGAGTCAC TGACTGCGAC AGCGAACTAC 
TCTAAAGCCG TGCACTGTAT ACTGGACGTG GTCCACGAGG TCCTGCATCA TCAGAGACGT 
CTGGAGAACA TCTGGCAGCA CCGTAAAGTC CGTCTGCATC AGAGACTGCA GCTCTGTGTC 
TTCCAGCAGG ACGTCCAACA GGTGATTGAC TGGATAGAGA ATCACGGGGA GGCTTTCCTC 
AGCAAACACA CTGGTGTGGG CAAATCCCTG CACAGAGCCA GAGCCCTCCA GAAACGCCAT 
GATGACTTTG AGGAGGTGGC ACAGAATACA TACACTAATG CAGATAAGCT GCTGGAAGCT 
GCTGAGCAGT TGGCTCAGAC GGGCGAGTGT GACCCTGAGG AGATCTACAA GGCAGCGCGA 
CACCTGGAAG TCAGGATCCA GGACTTTGTT AGGCGAGTGG AGCAAAGAAA ACTACTGCTG 
GACATGTCTG TCTCTTTCCA CACGCACACT AAAGAGTTGT GGACGTGGAT GGAGGACCTG 
CAGAAAGAGC TGCTGGAGGA AGTTTGTTCT GACTCAGTCG ATTCAGTTCA GGAGCTGATA 
AAACAGTTTC AGCAGCAGCA GACGGCCACG CTGGAGGCAA CACTCAATGT CATTAAAGAG 
GGAGAAGATC TTATGCAACA ACTCCGAGAT TCAGCAATGA GCAGTAATAA GATGCCCCAC 
ACCAGCTCCA TCGCTCACAT TGAGGGCGTC CTGCAGCAGC TAGAGGAGGC GCAGGGCCAC 
ATGGAGGAAC TCTTCCATGA GAGAAAGATC AAACTCGACA TCTTCCTGCA ACTGCGCATC 
TTTGAGCAGT ACACCATTGA GGTAACGGCA GAGTTAGACG CGTGGAATGA AGACTTGGTT 
CGTCAGATGA ATGAGTATAC TGGAGAAGAA CCCAGTCTGG CTGAGCAGAG ACTCCAGCGG 
CACGCAGAGA GGAAACTGGC CATGACTAAC ATGACCTACG AAGTTATGCA GCAGGGTCAG 
GACCTGCATC AGTACATCAT GGAAGTCCAA GCTTCAGGTA TTGAGTTGAC CGGAGAGAAG 
GAGGTGGATC TGGCAACACA AGTTCAGGAG CTGCTGGAGT TCCTTCATGA GAAACAGCAC 
GAGTTGGACA TGAGCGCCGA ACACACACAG AAGCGCCTGG AGCAGTGTGT GCAGCTCCGC 
CACCTGCAGG CTGAGGTTAA ACAGGTTCTG GGGTGGATTC GTAATGGTGA ATCTATGTTG 
GATGCCAGTA TGGTGAATGC AAGTTCACTG TCAGAGGCCG AACAACTACA GAGAGAACAT 
GAACAGTTCC AGCTGGCCAT AGAGTCTTTG TTCCACGCTA ACTCTCTCCA GAAAACCCAT 
CAGAGTGCAC TACAGGTCCA GCAGAAGGCA GAAATCATGT TACAGGCTGG ACACTTTGAC 
CCCGAGGCCA TCCGCAGCTG TGCAGAGAAG GTGGCCGTGC ACTGGCAGCA GCTCATGCTC 
AAGATGGAGG ACAGACTCAA ACTGGTTAAT GCCTCGGTGG CCTTCTACAA GACGTCAGAA 
CAGGTGTGTA GTGTCCTGGA GAGTCTGGAG CAGGAGTACC GCAGAGATGA AGACTGGTGT 
GGAGCTCATG ATAAACTAGG ATCTTCATCA GAGACTGATC ACGTTCTGCC TCTCATCAGC 
AAACACCTGG AGCAGAAAGA AGCTTTTCTC AAGGCATGCA CTCTGGCCAG GCGCAATGCT 
GAGGTTTTTC TGAAATACAT CCACCGGAAC AATGTCAGCT CTCAGGGATC ATCTAGCAAC 
ACTCGTGGCC CTGAGCAACA GGTCAAAGCC ATCCTGAATG AACTGCTGCA GAGGGAAAAC 
AGAGTTTTGC ATTTCTGGAC CATGAAGAAG CGGCGTTTGG ATCAGTGTCA GCAGTATGTG 
GTGTTTGAAC GCAATGCCAA ACAGGCATTA GAGTGGATTG AGGAGGCAGG AGAGGTCTAT 
CTGGCCACTC ACACCTGCAT CGGAGACTCT ATGGAGAAAA CTCAGGGACT CCTCAAAGAA 
TACGAAGAAT TCTGCATCTC CGCAAAGCAA ACTAAGGAGA AAGTGAAGAT GCTGATCCAG 
CTGGCGGATA ACTTTGTTGA GAAAGGACAC ATGCATGTCA CCGACTTGAA GAAGTGTCTG 
ACGGCAGTGG ACAAGCGCTA CAGGGACTTC TCCATGCGCA TGGGCAAATA CCGCTGTGAC 
CTGGAAAATG TGCTGGGCAT CAGCTCTGAG GATAATAAGG ATCTGGAGCT GGATATCATC 
CCAGCCAGTC TGACTGGCCC AGAGGTTAAA CTCCGTGACC CCAACCACGA GGTGAATGAA 
GAAAAGAGGA AATCAGCCCG GAAGAAAGAG TTTATTATAG CAGAGCTGTT GCAGACAGAA 
AAGGCTTATG TGAGGGACTT ACATGAATGC TTAGAGACAT ATTTGTGGGA GATGACCAAT 
GGTGTGGAGG AAATTCCTCC AGGAATCGCC AACAAAGAGC ACATCATCTT TGGCAACATT 
CAGGAGATTT ACGACTTCCA CAATAATATA TTCCTGAAGG AGCTGATCAA TTATGAGCAA 
CTGCCTGAGG ATGTGGGACA TTGCTTTGTG ACATGGGCCG ATAAGTTTAA CATTTATGTG 
ACATACTGCA AAAACAAACC AGATTCCAGT CAGCTCATCC TAGACCATGC TGGGAGTTTT 
TTTGATGAGA TTCAGAAGAG ACACAGTCTG ACCAACTCTA TATCATCATA CCTCATCAAA 
CCTGTACAGA GGATCACCAA ATATCAGCTG CTGCTCAAGG AGCTGCTGTC CTGCTGTGAA 
GAAGGCAAAG GTGAAATTAA AGATGGCCTG GAGGTCATGC TGAGTGTGCC AAAGAGAGCC 
AATGACGCCA TGCACGTCAG CATGCTGGAG GGTTTTGATG CAAATTTGGA TGTTCAGGGT 
GAACTCATCC TCCAAGACAC CTTCCAGGTG TGGGACCCCA AATCACTAAT CCGGAAGGGG 
CGAGACCGCC ACCTCTTTCT CTTCGAGATC TCCCTGGTCT TCAGTAAAGA GATCAAAGAC 
TCAGCAGGGC GATCCAAATA CATCTTTAAA AACAAACTAC TGACTTCTGA ATTGGGGGTG 
ACAGAGCATG TAGAGGGGGA CCCGTGCAAA TTTGCTCTGT GGGTGGGACG CACACCATCG 
TCTGACAACA AGACTGTCCT CAAGGCATCT AGTATTGAGG TGAAGCAAGA ATGGATTAAA 
AACATCCGAG AGGTCATTCA GGAGAGAATA ATTCACCTGA AGGGGGCGCT AAAGGAACCT 
ATCCACATTC CCAAAACTCC AGCCAAACCT CGCAATAACA GCAAGAGGGA CGCTGGTGAG 
GATGCTGACA GCCAGGGAGA CGGCAGCAGT CAGCCGGACA CCATCTCCAT AGCATCCAGA 
ACCTCTCAGA ACACTGTAGA CAGTGACAAG CTCTCTGGAG GGTGTGAGCT GACGGTGGTC 
CTGCAGGACT TTACAGCAAG CAGCTCATCA GAGCTCAGCA TCCAGACGGG TCAGACAGTG 
GAGCTGCTGG AGCGGCCCAG TGACCGTCCA GGATGGTGTC TGGTACGGAC CACCGAAAAG 
ACGCCTCCTC AAGAGGGACT GGTGCCCAGC AGTGCTCTGT GTATCTCGCA CTCACGCAGC 
AGTGTGGAGA TGGACTGCTT TTTCCCAGCA GGCAAAGAAA ACTATACAGT TAGTGGCCCA 
GAAGGAAAGA CAGAGTCAGT GGCCACACTG CAGCCACAGC CATCCCTGAA CTCTCTGCAG 
AGTAGCTCTC CTGGACCCAA GCGCTCTGGG AATACACTGA AGAAATGGCT GACCAGTCCT 
GTCCGACGTC TAAGTCATGG CAGTAACATC AAGAAGATCC CAAGTAAACA GAAACAGAAA 
CGTGATGGTC GCAAGAGTAT TGATATGGGG CCGCCCGAAC TACAGGATGA CACCTTGGAA 
GAGCGAGTGC AAAAAGAAGA GGGCACACTC TCCAAGTCTT CGTCTGGGAT GCACAGTGGA 
GGAGAGGAAG AGCCTGAGGA CGAGTCTCAC ACTCCTCTAC CTCCACCAAT GGAGATCATT 
AAAGACCCGT CGGCTCAGGA AGAGAAGACA CTGGAGCCAG GCTCATACCC GGGATTCAGC 
TCAGTCAATC CAGCAGAGCA GAGCCCGATT CCACCCAGAA ACCCAGAACT AGAGCAGAAA 
GCCAAAGCGC TACGAGGACG AATGTTTGTA GTCAAGGAAT TAATCCAAAC GGAAAAAGAC 
TATGTTAAAG ATCTGGGAAT TGTGGTTGAG GGGTTTATGA AGAGAATCGA GGAGAAAGGA 
GTTCCTGATA ACATGAAAGG AAAAGATAAG ATCGTCTTCG GAAACATTCA CCAAATCTAT 
GACTGGCACA AAGATTTTTT TGTTGGAGAA CTGGAGAAAT GCCTGGAAGA TCACGAAAAA 
CTGCCTGAAC TGTTCTGTAA ACATGAGAGA CGGTTACACA TGTATGTAGT TTACTGTCAG 
AACAAACCCA AATCCGAGTT CATCGTTGCA GAATATGATT CATACTTTGA GGGGATTCAG 
CAAGACATTA ACTCCAGACT GAGCATCAGC GATTTTCTCA TTAAACCCAT TCAGAGAATT 
ACTAAATACC AGCTTTTATT AAAGGATGTT TTGAAGTACA GTACAAAAGC AGGACTGGAT 
TGTCAGGATA TAGAGAATGC AGTTGACCTG ATGTCCCAGG TTCCCAAGCT TTGCAATGAC 
ATGATGAATC TGGGAAGACT GCAGGGATAT GAGGGGAAGT TGACGAGTCA AGGAAAACTC 
TTGCAGCAGG AGACGTTCTT TGTGACCGAG CAGGACTCTG GCGTTCTGTC CCGCAGTAAA 
GAGCGCAGGG TTTTCCTGTT CGAACAAATT GTCATCTTCA GCGAGCTGTT ACGTAAAGGC 
TCTTCCACTC CAGGGTACCA GTTCAAGAAG AGCATAAAGG TGAGCTTCCT TGACTTGGAA 
GAGCATGTTG AAAATGATCC ATGCAAGTTT GTGCTGTCGT GTCGTGGGTC GGCAGAACGT 
TTTACCCTTC AAGCAGCAAA CACGGACATT AAACAAGTCT GGGTTCAGCA TATCACCCAA 
CTACTAGATT TACAAAGCAA CTTCTTGTCA GCTCTGCAGT CTCCTATTGA GTATCAGAAG 
GAGCGTAGTG GCTCTCAATC TCTGACCCGG AACTCTTCTG GGCGGATGGG TCAGTCCAAC 
AGCAGGCCCT GCTCCACGGT TTCACTCGGA GCAGAGAAAC CCTCTCTGTC TGGACGGAGC 
TCCACCCCGC TAAAGCTATC TACCTCCAAT GGTGGTCCAT GCTCTGAAAG ATACCGCAGG 
CAGTTTGAAG GCTTGGGCTG CAACGGTACA TCGTCCTCCT TGATGGTGAC TCAAGATTAT 
ACTGCACTGA AAGAGAATGA GATCTGTGTC ACACAGGGTG AGGCCGTGCA GGTACTGGCC 
ACTAACCAGC AGAACATGTA CCTTGTGTAC CGACCCGCAA ATAGCCAATC ACCACCTGCA 
GAGGGCTGGG TGCCGGGACA CATCCTAGGC CCCGTCACAA AACCTCTCAT AGACACTACT 
GCAGACGCAA GCATCAAGAA ATCTCTTTCG TGGAACACAC TTCGTGCTCG TAAGCGTGCA 
GAAAAGGACA GCACGCCCCT CAAAAGTGAC TTTAAAGTTG AGAATGGACT GCGTAAGCCA 
AAGGAAATTC TCAGCAGCAA AGTCAAAGTG AAAGAGTCCC GCAGTTCTGA TGAGTCTGAA 
TGTGAGGATG AACTAGACCC CAAAACTAGT ATGGAGTTAC TGAACCCTAA TTTCATCCAA 
GAGGTCGCTC CAGAGTTTCT GACGCCTCTA ACAGACGTGA CGTGTGCTCT GGGAGAGACG 
GTGGTTCTGT GCTGTAAAGT GTGTGCTCGA CCCAAACCCA CAATCACCTT GAAAGGACCT 
GATCAAAGTC TGCTGGCCAA CAATAACCGC TTCACTATTA ATATCAGAGA AACCGGTGAT 
ATAGTGTTGA AAATCTGCAA TCTAATGCCG CAGGACACTG GCATCTACAC GTGTGTTGCT 
GTGAATGACC ATGGTACAGC CTCCTCATCT GCATCCATTA AAGTACAAGG TATCCCAGCA 
GCCCCTGCTC GACCGGTGGC ACAGGAGGCA AGCAGCACTG CAGTGATTGT CCACTGGCTT 
CCTCCAGCCA GCTCAGCAAA TTCTGCCATC TCCAGCTACA CTGTGGAATA TAGGCAGGAA 
GATTCTCTGG TATGGCAGCA GTTGGTGGTC TCCACGGCAG ATGTGTTTGT GAAGATTGAC 
AATCTGATTC CTGGTGGGCA CTACCAGTTC AGAGTCAGTG CCAGTAATCC CTGGGGCACC 
AGCCCGCCTA GTGAACCCTC AAACATGGTG ACATTATCCA GCACAGCATC AACATATGAT 
GGCGCTGGAA TCCAGTGGAA AGACAACTTT GAATCTGCTT TTACAGAATT ATGTGAAATT 
GGGCGGGGTC GTTTCTCTGT GGTGAGGAAG TGTCTCAGTA AAGCCACTAA GCAGGAGGTT 
GCTGTGAAGT TTGTGAATAA GAAGATGCAC AAGAAAGAAC AGGTGGCCCA TGAAGCTGAT 
ATTCTACGCC ATGTCCAGCA CCCCCAACTG GTGTCACTGA TTGATACATA CGAGTCGCCT 
ACAGCCTACA TGCTGATTCT TGAACTTGTT GAGGATGGAC GTCTTCTTGA CTATCTGGTA 
GCTCATGATG AGCTGATGGA GGAGAAAGTG GCATTCTTTA TTAAAGATAC ACTGGAAGCT 
CTGCAGCACT TGCACACCTG CAGAGTGGCT CATCTAGACC TCAAGCCTGA AAACTTGTTG 
GTGGACCTCC ACGTTCCGGT ACCCTGCGTG AAGCTCTCAG ATCTAGGTGA TGCTGTACAG 
GTTTCAAGTC ACCGCTACGT ACATCTCCTC CTGGGTAACC CCGAGTTCGC TGCTCCAGAG 
CTCATCCAGG GCACTCCAGT GTCCCTCTCT ACAGATGTGT GGAGTGTAGG GGTGCTGGCC 
TACGTCATGC TTAGTGGAGT CTCCCCATTC CTGGATGAGA GTCTGGAGGA GACCTGCGTC 
AACATCTGCA GGCTGGACTT CTGCTTTCCT CAAGAGTACT TCTCCGATAT AAGCCAGGCA 
GCCAAAGACT TCATTGTGTC CACGCTTAAC CAGGATCCCA GGAAGAGGCC AAGCTCGGCC 
TCGTGTCTTC AGCACCCATG GGTCAGTGCT CATAGTGGAG ATTACTCCAA AACACCTCTG 
GATACTGTCA GACTCGCTGC ATTTATAGAC AGACGCAAAC AGCTACATGA TGTCAGGCCT 
GTCACAAACA TTAAAGGAAT GGTCAGCAGC AGTATGGGAC ACACCTTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

kalrna ( XM_009304451.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009302726.2 Danio rerio kalirin, RhoGEF kinase a (kalrna), transcript variant X3, mRNA.

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CloneID	ODa60289
Clone ID Related Accession (Same CDS sequence)	XM_009304451.3 , XM_009304451.2
Accession Version	XM_009304451.2	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 8811bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	kalirin isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007120.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009304451.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATTCTG CTGAAGCGCC GGAGGACGGA CCCAATGATT CGGACACGGA TGCATTCTTC 
AAAACAGGGT CTTTCAGAAG TGAAGGAATT AAAGCCTCTG ATGTCCTTCC TGTGCTGAGA 
GAAAAAATAG CATTTGTGTC AGGCGGTCGT GATAAGCGAG GTGGCCCTAT ACTGACCTTT 
CCTGCAAGGA GTAACCATGA CCGAATAAAA CAAGAGGACC TGAGACGGCT CGTAACATAC 
CTGTCCACTG TGCCCAGTGA GGACGTTTGT AAGAGAGGCT TCACTGTGAT TATTGATATG 
CGTGGCTCTA AATGGGAGCT AATAAAGCCA CTGCTGAAGA CGCTACAGGA AGCATTTCCA 
GCTGAGATCT GCGTCGCTCT CATCATCAAA CCAGACAACT TCTGGCAGAA GCAGAAGACT 
AACTTTGGTA GCGCCAAGTT CACCTTTGAG ACTAGCATGG TATCAGTGGA GGGCTTGACC 
AAACTAGTGG ACCCATCCCA GCTAACGGAC GACTTTGAAG GTTCTCTGGA ATACAATCAT 
GAGGAATGGC TTGAGCTCCG GGTGGCTCTG GAGGAGTTCC TGGCCAGCGC CGTTCACCTC 
CTCTCTCGCC TGGAAGATCT ACAGGAGACT CTGGCTAAGA AGGAGTTCCC GGTGGATGTG 
GAAGCCTCTC GTAGACTCAT CGATGAGCAC ACACAGCTGA AGAAGAAGGT GATGAAGGCT 
CCGGTGGAAG AGCTGGACCA CGAAGGCCAG AGACTCTTGC AGTGCATCCG CTCCAGCGAC 
GGCTTCTCAG GGAGGAACTG CATTTCTGGA AGTGCCGACT TCCAGAGCGT GGTTCCGAAG 
ATCGCCAGTC TGCTGGATAA ACTCCACAGC ACTCGACAGC ACTTGCACCA GATGTGGCAC 
CTACGAAAAC TCAAGCTGGA TCAGTGCTTT CAGCTCCGAC TGTTTGAACA GGACGCAGAG 
AAGATGTTTG ACTGGATCAG TCACAATAAG GAATTGTTTC TTCAGAGCCA CACGGAGATC 
GGTGTGAGCT ACCAGCATGC TGTGGAGCTA CAGACGCAAC ACAACCACTT TGCCATGAAC 
TCAATGAATG CCTATGTGAA CATAAACCGC ATCATGTCGG TGGCGTCGCG GCTGTCGGAG 
GCGGGCCACT ACGCCGCTCA GCAGATCAAA CAGATCAGCA CTCAGCTGGA TCAGGAGTGG 
AAGAGCTTCG CGGCTGCTCT CGATGAACGC AGCACCATCC TTGCCATGTC TGCCGTCTTC 
CATCAGAAAT CTGAACAGTT TTTGTCCGGT GTGGAAACAT GGTGTAAGAT GTGCAGTGAA 
GGCGGCTTGC CATCAGAGAT GCAGGATCTG GAATCGGCCA TTCATCACCA TCAGACGCTC 
TATGAACAAG TCACACAAGC ATACACCGAG GTGAGTCAGG ACGGTAAGGC TTTGCTGGAC 
GTTCTTCAGC GGCCGTTGAG TCCTGGGAAC GCAGAGTCAC TGACTGCGAC AGCGAACTAC 
TCTAAAGCCG TGCACTGTAT ACTGGACGTG GTCCACGAGG TCCTGCATCA TCAGAGACGT 
CTGGAGAACA TCTGGCAGCA CCGTAAAGTC CGTCTGCATC AGAGACTGCA GCTCTGTGTC 
TTCCAGCAGG ACGTCCAACA GGTGATTGAC TGGATAGAGA ATCACGGGGA GGCTTTCCTC 
AGCAAACACA CTGGTGTGGG CAAATCCCTG CACAGAGCCA GAGCCCTCCA GAAACGCCAT 
GATGACTTTG AGGAGGTGGC ACAGAATACA TACACTAATG CAGATAAGCT GCTGGAAGCT 
GCTGAGCAGT TGGCTCAGAC GGGCGAGTGT GACCCTGAGG AGATCTACAA GGCAGCGCGA 
CACCTGGAAG TCAGGATCCA GGACTTTGTT AGGCGAGTGG AGCAAAGAAA ACTACTGCTG 
GACATGTCTG TCTCTTTCCA CACGCACACT AAAGAGTTGT GGACGTGGAT GGAGGACCTG 
CAGAAAGAGC TGCTGGAGGA AGTTTGTTCT GACTCAGTCG ATTCAGTTCA GGAGCTGATA 
AAACAGTTTC AGCAGCAGCA GACGGCCACG CTGGAGGCAA CACTCAATGT CATTAAAGAG 
GGAGAAGATC TTATGCAACA ACTCCGAGAT TCAGCAATGA GCAGTAATAA GATGCCCCAC 
ACCAGCTCCA TCGCTCACAT TGAGGGCGTC CTGCAGCAGC TAGAGGAGGC GCAGGGCCAC 
ATGGAGGAAC TCTTCCATGA GAGAAAGATC AAACTCGACA TCTTCCTGCA ACTGCGCATC 
TTTGAGCAGT ACACCATTGA GGTAACGGCA GAGTTAGACG CGTGGAATGA AGACTTGGTT 
CGTCAGATGA ATGAGTATAC TGGAGAAGAA CCCAGTCTGG CTGAGCAGAG ACTCCAGCGG 
CACGCAGAGA GGAAACTGGC CATGACTAAC ATGACCTACG AAGTTATGCA GCAGGGTCAG 
GACCTGCATC AGTACATCAT GGAAGTCCAA GCTTCAGGTA TTGAGTTGAC CGGAGAGAAG 
GAGGTGGATC TGGCAACACA AGTTCAGGAG CTGCTGGAGT TCCTTCATGA GAAACAGCAC 
GAGTTGGACA TGAGCGCCGA ACACACACAG AAGCGCCTGG AGCAGTGTGT GCAGCTCCGC 
CACCTGCAGG CTGAGGTTAA ACAGGTTCTG GGGTGGATTC GTAATGGTGA ATCTATGTTG 
GATGCCAGTA TGGTGAATGC AAGTTCACTG TCAGAGGCCG AACAACTACA GAGAGAACAT 
GAACAGTTCC AGCTGGCCAT AGAGTCTTTG TTCCACGCTA ACTCTCTCCA GAAAACCCAT 
CAGAGTGCAC TACAGGTCCA GCAGAAGGCA GAAATCATGT TACAGGCTGG ACACTTTGAC 
CCCGAGGCCA TCCGCAGCTG TGCAGAGAAG GTGGCCGTGC ACTGGCAGCA GCTCATGCTC 
AAGATGGAGG ACAGACTCAA ACTGGTTAAT GCCTCGGTGG CCTTCTACAA GACGTCAGAA 
CAGGTGTGTA GTGTCCTGGA GAGTCTGGAG CAGGAGTACC GCAGAGATGA AGACTGGTGT 
GGAGCTCATG ATAAACTAGG ATCTTCATCA GAGACTGATC ACGTTCTGCC TCTCATCAGC 
AAACACCTGG AGCAGAAAGA AGCTTTTCTC AAGGCATGCA CTCTGGCCAG GCGCAATGCT 
GAGGTTTTTC TGAAATACAT CCACCGGAAC AATGTCAGCT CTCAGGGATC ATCTAGCAAC 
ACTCGTGGCC CTGAGCAACA GGTCAAAGCC ATCCTGAATG AACTGCTGCA GAGGGAAAAC 
AGAGTTTTGC ATTTCTGGAC CATGAAGAAG CGGCGTTTGG ATCAGTGTCA GCAGTATGTG 
GTGTTTGAAC GCAATGCCAA ACAGGCATTA GAGTGGATTG AGGAGGCAGG AGAGGTCTAT 
CTGGCCACTC ACACCTGCAT CGGAGACTCT ATGGAGAAAA CTCAGGGACT CCTCAAAGAA 
TACGAAGAAT TCTGCATCTC CGCAAAGCAA ACTAAGGAGA AAGTGAAGAT GCTGATCCAG 
CTGGCGGATA ACTTTGTTGA GAAAGGACAC ATGCATGTCA CCGACTTGAA GAAGTGTCTG 
ACGGCAGTGG ACAAGCGCTA CAGGGACTTC TCCATGCGCA TGGGCAAATA CCGCTGTGAC 
CTGGAAAATG TGCTGGGCAT CAGCTCTGAG GATAATAAGG ATCTGGAGCT GGATATCATC 
CCAGCCAGTC TGACTGGCCC AGAGGTTAAA CTCCGTGACC CCAACCACGA GGTGAATGAA 
GAAAAGAGGA AATCAGCCCG GAAGAAAGAG TTTATTATAG CAGAGCTGTT GCAGACAGAA 
AAGGCTTATG TGAGGGACTT ACATGAATGC TTAGAGACAT ATTTGTGGGA GATGACCAAT 
GGTGTGGAGG AAATTCCTCC AGGAATCGCC AACAAAGAGC ACATCATCTT TGGCAACATT 
CAGGAGATTT ACGACTTCCA CAATAATATA TTCCTGAAGG AGCTGATCAA TTATGAGCAA 
CTGCCTGAGG ATGTGGGACA TTGCTTTGTG ACATGGGCCG ATAAGTTTAA CATTTATGTG 
ACATACTGCA AAAACAAACC AGATTCCAGT CAGCTCATCC TAGACCATGC TGGGAGTTTT 
TTTGATGAGA TTCAGAAGAG ACACAGTCTG ACCAACTCTA TATCATCATA CCTCATCAAA 
CCTGTACAGA GGATCACCAA ATATCAGCTG CTGCTCAAGG AGCTGCTGTC CTGCTGTGAA 
GAAGGCAAAG GTGAAATTAA AGATGGCCTG GAGGTCATGC TGAGTGTGCC AAAGAGAGCC 
AATGACGCCA TGCACGTCAG CATGCTGGAG GGTTTTGATG CAAATTTGGA TGTTCAGGGT 
GAACTCATCC TCCAAGACAC CTTCCAGGTG TGGGACCCCA AATCACTAAT CCGGAAGGGG 
CGAGACCGCC ACCTCTTTCT CTTCGAGATC TCCCTGGTCT TCAGTAAAGA GATCAAAGAC 
TCAGCAGGGC GATCCAAATA CATCTTTAAA AACAAACTAC TGACTTCTGA ATTGGGGGTG 
ACAGAGCATG TAGAGGGGGA CCCGTGCAAA TTTGCTCTGT GGGTGGGACG CACACCATCG 
TCTGACAACA AGACTGTCCT CAAGGCATCT AGTATTGAGG TGAAGCAAGA ATGGATTAAA 
AACATCCGAG AGGTCATTCA GGAGAGAATA ATTCACCTGA AGGGGGCGCT AAAGGAACCT 
ATCCACATTC CCAAAACTCC AGCCAAACCT CGCAATAACA GCAAGAGGGA CGCTGGTGAG 
GATGCTGACA GCCAGGGAGA CGGCAGCAGT CAGCCGGACA CCATCTCCAT AGCATCCAGA 
ACCTCTCAGA ACACTGTAGA CAGTGACAAG CTCTCTGGAG GGTGTGAGCT GACGGTGGTC 
CTGCAGGACT TTACAGCAAG CAGCTCATCA GAGCTCAGCA TCCAGACGGG TCAGACAGTG 
GAGCTGCTGG AGCGGCCCAG TGACCGTCCA GGATGGTGTC TGGTACGGAC CACCGAAAAG 
ACGCCTCCTC AAGAGGGACT GGTGCCCAGC AGTGCTCTGT GTATCTCGCA CTCACGCAGC 
AGTGTGGAGA TGGACTGCTT TTTCCCAGCA GGCAAAGAAA ACTATACAGT TAGTGGCCCA 
GAAGGAAAGA CAGAGTCAGT GGCCACACTG CAGCCACAGC CATCCCTGAA CTCTCTGCAG 
AGTAGCTCTC CTGGACCCAA GCGCTCTGGG AATACACTGA AGAAATGGCT GACCAGTCCT 
GTCCGACGTC TAAGTCATGG CAGTAACATC AAGAAGATCC CAAGTAAACA GAAACAGAAA 
CGTGATGGTC GCAAGAGTAT TGATATGGGG CCGCCCGAAC TACAGGATGA CACCTTGGAA 
GAGCGAGTGC AAAAAGAAGA GGGCACACTC TCCAAGTCTT CGTCTGGGAT GCACAGTGGA 
GGAGAGGAAG AGCCTGAGGA CGAGTCTCAC ACTCCTCTAC CTCCACCAAT GGAGATCATT 
AAAGACCCGT CGGCTCAGGA AGAGAAGACA CTGGAGCCAG GCTCATACCC GGGATTCAGC 
TCAGTCAATC CAGCAGAGCA GAGCCCGATT CCACCCAGAA ACCCAGAACT AGAGCAGAAA 
GCCAAAGCGC TACGAGGACG AATGTTTGTA GTCAAGGAAT TAATCCAAAC GGAAAAAGAC 
TATGTTAAAG ATCTGGGAAT TGTGGTTGAG GGGTTTATGA AGAGAATCGA GGAGAAAGGA 
GTTCCTGATA ACATGAAAGG AAAAGATAAG ATCGTCTTCG GAAACATTCA CCAAATCTAT 
GACTGGCACA AAGATTTTTT TGTTGGAGAA CTGGAGAAAT GCCTGGAAGA TCACGAAAAA 
CTGCCTGAAC TGTTCTGTAA ACATGAGAGA CGGTTACACA TGTATGTAGT TTACTGTCAG 
AACAAACCCA AATCCGAGTT CATCGTTGCA GAATATGATT CATACTTTGA GGGGATTCAG 
CAAGACATTA ACTCCAGACT GAGCATCAGC GATTTTCTCA TTAAACCCAT TCAGAGAATT 
ACTAAATACC AGCTTTTATT AAAGGATGTT TTGAAGTACA GTACAAAAGC AGGACTGGAT 
TGTCAGGATA TAGAGAATGC AGTTGACCTG ATGTCCCAGG TTCCCAAGCT TTGCAATGAC 
ATGATGAATC TGGGAAGACT GCAGGGATAT GAGGGGAAGT TGACGAGTCA AGGAAAACTC 
TTGCAGCAGG AGACGTTCTT TGTGACCGAG CAGGACTCTG GCGTTCTGTC CCGCAGTAAA 
GAGCGCAGGG TTTTCCTGTT CGAACAAATT GTCATCTTCA GCGAGCTGTT ACGTAAAGGC 
TCTTCCACTC CAGGGTACCA GTTCAAGAAG AGCATAAAGG TGAGCTTCCT TGACTTGGAA 
GAGCATGTTG AAAATGATCC ATGCAAGTTT GTGCTGTCGT GTCGTGGGTC GGCAGAACGT 
TTTACCCTTC AAGCAGCAAA CACGGACATT AAACAAGTCT GGGTTCAGCA TATCACCCAA 
CTACTAGATT TACAAAGCAA CTTCTTGTCA GCTCTGCAGT CTCCTATTGA GTATCAGAAG 
GAGCGTAGTG GCTCTCAATC TCTGACCCGG AACTCTTCTG GGCGGATGGG TCAGTCCAAC 
AGCAGGCCCT GCTCCACGGT TTCACTCGGA GCAGAGAAAC CCTCTCTGTC TGGACGGAGC 
TCCACCCCGC TAAAGCTATC TACCTCCAAT GGTGGTCCAT GCTCTGAAAG ATACCGCAGG 
CAGTTTGAAG GCTTGGGCTG CAACGGTACA TCGTCCTCCT TGATGGTGAC TCAAGATTAT 
ACTGCACTGA AAGAGAATGA GATCTGTGTC ACACAGGGTG AGGCCGTGCA GGTACTGGCC 
ACTAACCAGC AGAACATGTA CCTTGTGTAC CGACCCGCAA ATAGCCAATC ACCACCTGCA 
GAGGGCTGGG TGCCGGGACA CATCCTAGGC CCCGTCACAA AACCTCTCAT AGACACTACT 
GCAGACGCAA GCATCAAGAA ATCTCTTTCG TGGAACACAC TTCGTGCTCG TAAGCGTGCA 
GAAAAGGACA GCACGCCCCT CAAAAGTGAC TTTAAAGTTG AGAATGGACT GCGTAAGCCA 
AAGGAAATTC TCAGCAGCAA AGTCAAAGTG AAAGAGTCCC GCAGTTCTGA TGAGTCTGAA 
TGTGAGGATG AACTAGACCC CAAAACTAGT ATGGAGTTAC TGAACCCTAA TTTCATCCAA 
GAGGTCGCTC CAGAGTTTCT GACGCCTCTA ACAGACGTGA CGTGTGCTCT GGGAGAGACG 
GTGGTTCTGT GCTGTAAAGT GTGTGCTCGA CCCAAACCCA CAATCACCTT GAAAGGACCT 
GATCAAAGTC TGCTGGCCAA CAATAACCGC TTCACTATTA ATATCAGAGA AACCGGTGAT 
ATAGTGTTGA AAATCTGCAA TCTAATGCCG CAGGACACTG GCATCTACAC GTGTGTTGCT 
GTGAATGACC ATGGTACAGC CTCCTCATCT GCATCCATTA AAGTACAAGG TATCCCAGCA 
GCCCCTGCTC GACCGGTGGC ACAGGAGGCA AGCAGCACTG CAGTGATTGT CCACTGGCTT 
CCTCCAGCCA GCTCAGCAAA TTCTGCCATC TCCAGCTACA CTGTGGAATA TAGGCAGGAA 
GATTCTCTGG TATGGCAGCA GTTGGTGGTC TCCACGGCAG ATGTGTTTGT GAAGATTGAC 
AATCTGATTC CTGGTGGGCA CTACCAGTTC AGAGTCAGTG CCAGTAATCC CTGGGGCACC 
AGCCCGCCTA GTGAACCCTC AAACATGGTG ACATTATCCA GCACAGCATC AACATATGAT 
GGCGCTGGAA TCCAGTGGAA AGACAACTTT GAATCTGCTT TTACAGAATT ATGTGAAATT 
GGGCGGGGTC GTTTCTCTGT GGTGAGGAAG TGTCTCAGTA AAGCCACTAA GCAGGAGGTT 
GCTGTGAAGT TTGTGAATAA GAAGATGCAC AAGAAAGAAC AGGTGGCCCA TGAAGCTGAT 
ATTCTACGCC ATGTCCAGCA CCCCCAACTG GTGTCACTGA TTGATACATA CGAGTCGCCT 
ACAGCCTACA TGCTGATTCT TGAACTTGTT GAGGATGGAC GTCTTCTTGA CTATCTGGTA 
GCTCATGATG AGCTGATGGA GGAGAAAGTG GCATTCTTTA TTAAAGATAC ACTGGAAGCT 
CTGCAGCACT TGCACACCTG CAGAGTGGCT CATCTAGACC TCAAGCCTGA AAACTTGTTG 
GTGGACCTCC ACGTTCCGGT ACCCTGCGTG AAGCTCTCAG ATCTAGGTGA TGCTGTACAG 
GTTTCAAGTC ACCGCTACGT ACATCTCCTC CTGGGTAACC CCGAGTTCGC TGCTCCAGAG 
CTCATCCAGG GCACTCCAGT GTCCCTCTCT ACAGATGTGT GGAGTGTAGG GGTGCTGGCC 
TACGTCATGC TTAGTGGAGT CTCCCCATTC CTGGATGAGA GTCTGGAGGA GACCTGCGTC 
AACATCTGCA GGCTGGACTT CTGCTTTCCT CAAGAGTACT TCTCCGATAT AAGCCAGGCA 
GCCAAAGACT TCATTGTGTC CACGCTTAAC CAGGATCCCA GGAAGAGGCC AAGCTCGGCC 
TCGTGTCTTC AGCACCCATG GGTCAGTGCT CATAGTGGAG ATTACTCCAA AACACCTCTG 
GATACTGTCA GACTCGCTGC ATTTATAGAC AGACGCAAAC AGCTACATGA TGTCAGGCCT 
GTCACAAACA TTAAAGGAAT GGTCAGCAGC AGTATGGGAC ACACCTTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009302726.2
CDS	236..9046
Translation	MNSAEAPEDGPNDSDTDAFFKTGSFRSEGIKASDVLPVLREKIAFVSGGRDKRGGPILTFPARSNHDRIKQEDLRRLVTYLSTVPSEDVCKRGFTVIIDMRGSKWELIKPLLKTLQEAFPAEICVALIIKPDNFWQKQKTNFGSAKFTFETSMVSVEGLTKLVDPSQLTDDFEGSLEYNHEEWLELRVALEEFLASAVHLLSRLEDLQETLAKKEFPVDVEASRRLIDEHTQLKKKVMKAPVEELDHEGQRLLQCIRSSDGFSGRNCISGSADFQSVVPKIASLLDKLHSTRQHLHQMWHLRKLKLDQCFQLRLFEQDAEKMFDWISHNKELFLQSHTEIGVSYQHAVELQTQHNHFAMNSMNAYVNINRIMSVASRLSEAGHYAAQQIKQISTQLDQEWKSFAAALDERSTILAMSAVFHQKSEQFLSGVETWCKMCSEGGLPSEMQDLESAIHHHQTLYEQVTQAYTEVSQDGKALLDVLQRPLSPGNAESLTATANYSKAVHCILDVVHEVLHHQRRLENIWQHRKVRLHQRLQLCVFQQDVQQVIDWIENHGEAFLSKHTGVGKSLHRARALQKRHDDFEEVAQNTYTNADKLLEAAEQLAQTGECDPEEIYKAARHLEVRIQDFVRRVEQRKLLLDMSVSFHTHTKELWTWMEDLQKELLEEVCSDSVDSVQELIKQFQQQQTATLEATLNVIKEGEDLMQQLRDSAMSSNKMPHTSSIAHIEGVLQQLEEAQGHMEELFHERKIKLDIFLQLRIFEQYTIEVTAELDAWNEDLVRQMNEYTGEEPSLAEQRLQRHAERKLAMTNMTYEVMQQGQDLHQYIMEVQASGIELTGEKEVDLATQVQELLEFLHEKQHELDMSAEHTQKRLEQCVQLRHLQAEVKQVLGWIRNGESMLDASMVNASSLSEAEQLQREHEQFQLAIESLFHANSLQKTHQSALQVQQKAEIMLQAGHFDPEAIRSCAEKVAVHWQQLMLKMEDRLKLVNASVAFYKTSEQVCSVLESLEQEYRRDEDWCGAHDKLGSSSETDHVLPLISKHLEQKEAFLKACTLARRNAEVFLKYIHRNNVSSQGSSSNTRGPEQQVKAILNELLQRENRVLHFWTMKKRRLDQCQQYVVFERNAKQALEWIEEAGEVYLATHTCIGDSMEKTQGLLKEYEEFCISAKQTKEKVKMLIQLADNFVEKGHMHVTDLKKCLTAVDKRYRDFSMRMGKYRCDLENVLGISSEDNKDLELDIIPASLTGPEVKLRDPNHEVNEEKRKSARKKEFIIAELLQTEKAYVRDLHECLETYLWEMTNGVEEIPPGIANKEHIIFGNIQEIYDFHNNIFLKELINYEQLPEDVGHCFVTWADKFNIYVTYCKNKPDSSQLILDHAGSFFDEIQKRHSLTNSISSYLIKPVQRITKYQLLLKELLSCCEEGKGEIKDGLEVMLSVPKRANDAMHVSMLEGFDANLDVQGELILQDTFQVWDPKSLIRKGRDRHLFLFEISLVFSKEIKDSAGRSKYIFKNKLLTSELGVTEHVEGDPCKFALWVGRTPSSDNKTVLKASSIEVKQEWIKNIREVIQERIIHLKGALKEPIHIPKTPAKPRNNSKRDAGEDADSQGDGSSQPDTISIASRTSQNTVDSDKLSGGCELTVVLQDFTASSSSELSIQTGQTVELLERPSDRPGWCLVRTTEKTPPQEGLVPSSALCISHSRSSVEMDCFFPAGKENYTVSGPEGKTESVATLQPQPSLNSLQSSSPGPKRSGNTLKKWLTSPVRRLSHGSNIKKIPSKQKQKRDGRKSIDMGPPELQDDTLEERVQKEEGTLSKSSSGMHSGGEEEPEDESHTPLPPPMEIIKDPSAQEEKTLEPGSYPGFSSVNPAEQSPIPPRNPELEQKAKALRGRMFVVKELIQTEKDYVKDLGIVVEGFMKRIEEKGVPDNMKGKDKIVFGNIHQIYDWHKDFFVGELEKCLEDHEKLPELFCKHERRLHMYVVYCQNKPKSEFIVAEYDSYFEGIQQDINSRLSISDFLIKPIQRITKYQLLLKDVLKYSTKAGLDCQDIENAVDLMSQVPKLCNDMMNLGRLQGYEGKLTSQGKLLQQETFFVTEQDSGVLSRSKERRVFLFEQIVIFSELLRKGSSTPGYQFKKSIKVSFLDLEEHVENDPCKFVLSCRGSAERFTLQAANTDIKQVWVQHITQLLDLQSNFLSALQSPIEYQKERSGSQSLTRNSSGRMGQSNSRPCSTVSLGAEKPSLSGRSSTPLKLSTSNGGPCSERYRRQFEGLGCNGTSSSLMVTQDYTALKENEICVTQGEAVQVLATNQQNMYLVYRPANSQSPPAEGWVPGHILGPVTKPLIDTTADASIKKSLSWNTLRARKRAEKDSTPLKSDFKVENGLRKPKEILSSKVKVKESRSSDESECEDELDPKTSMELLNPNFIQEVAPEFLTPLTDVTCALGETVVLCCKVCARPKPTITLKGPDQSLLANNNRFTINIRETGDIVLKICNLMPQDTGIYTCVAVNDHGTASSSASIKVQGIPAAPARPVAQEASSTAVIVHWLPPASSANSAISSYTVEYRQEDSLVWQQLVVSTADVFVKIDNLIPGGHYQFRVSASNPWGTSPPSEPSNMVTLSSTASTYDGAGIQWKDNFESAFTELCEIGRGRFSVVRKCLSKATKQEVAVKFVNKKMHKKEQVAHEADILRHVQHPQLVSLIDTYESPTAYMLILELVEDGRLLDYLVAHDELMEEKVAFFIKDTLEALQHLHTCRVAHLDLKPENLLVDLHVPVPCVKLSDLGDAVQVSSHRYVHLLLGNPEFAAPELIQGTPVSLSTDVWSVGVLAYVMLSGVSPFLDESLEETCVNICRLDFCFPQEYFSDISQAAKDFIVSTLNQDPRKRPSSASCLQHPWVSAHSGDYSKTPLDTVRLAAFIDRRKQLHDVRPVTNIKGMVSSSMGHTL

Target ORF information:

RefSeq Version	XM_009304451.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio kalirin, RhoGEF kinase a (kalrna), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009304451.2

ORF Insert Sequence:

ATGAATTCTG CTGAAGCGCC GGAGGACGGA CCCAATGATT CGGACACGGA TGCATTCTTC 
AAAACAGGGT CTTTCAGAAG TGAAGGAATT AAAGCCTCTG ATGTCCTTCC TGTGCTGAGA 
GAAAAAATAG CATTTGTGTC AGGCGGTCGT GATAAGCGAG GTGGCCCTAT ACTGACCTTT 
CCTGCAAGGA GTAACCATGA CCGAATAAAA CAAGAGGACC TGAGACGGCT CGTAACATAC 
CTGTCCACTG TGCCCAGTGA GGACGTTTGT AAGAGAGGCT TCACTGTGAT TATTGATATG 
CGTGGCTCTA AATGGGAGCT AATAAAGCCA CTGCTGAAGA CGCTACAGGA AGCATTTCCA 
GCTGAGATCT GCGTCGCTCT CATCATCAAA CCAGACAACT TCTGGCAGAA GCAGAAGACT 
AACTTTGGTA GCGCCAAGTT CACCTTTGAG ACTAGCATGG TATCAGTGGA GGGCTTGACC 
AAACTAGTGG ACCCATCCCA GCTAACGGAC GACTTTGAAG GTTCTCTGGA ATACAATCAT 
GAGGAATGGC TTGAGCTCCG GGTGGCTCTG GAGGAGTTCC TGGCCAGCGC CGTTCACCTC 
CTCTCTCGCC TGGAAGATCT ACAGGAGACT CTGGCTAAGA AGGAGTTCCC GGTGGATGTG 
GAAGCCTCTC GTAGACTCAT CGATGAGCAC ACACAGCTGA AGAAGAAGGT GATGAAGGCT 
CCGGTGGAAG AGCTGGACCA CGAAGGCCAG AGACTCTTGC AGTGCATCCG CTCCAGCGAC 
GGCTTCTCAG GGAGGAACTG CATTTCTGGA AGTGCCGACT TCCAGAGCGT GGTTCCGAAG 
ATCGCCAGTC TGCTGGATAA ACTCCACAGC ACTCGACAGC ACTTGCACCA GATGTGGCAC 
CTACGAAAAC TCAAGCTGGA TCAGTGCTTT CAGCTCCGAC TGTTTGAACA GGACGCAGAG 
AAGATGTTTG ACTGGATCAG TCACAATAAG GAATTGTTTC TTCAGAGCCA CACGGAGATC 
GGTGTGAGCT ACCAGCATGC TGTGGAGCTA CAGACGCAAC ACAACCACTT TGCCATGAAC 
TCAATGAATG CCTATGTGAA CATAAACCGC ATCATGTCGG TGGCGTCGCG GCTGTCGGAG 
GCGGGCCACT ACGCCGCTCA GCAGATCAAA CAGATCAGCA CTCAGCTGGA TCAGGAGTGG 
AAGAGCTTCG CGGCTGCTCT CGATGAACGC AGCACCATCC TTGCCATGTC TGCCGTCTTC 
CATCAGAAAT CTGAACAGTT TTTGTCCGGT GTGGAAACAT GGTGTAAGAT GTGCAGTGAA 
GGCGGCTTGC CATCAGAGAT GCAGGATCTG GAATCGGCCA TTCATCACCA TCAGACGCTC 
TATGAACAAG TCACACAAGC ATACACCGAG GTGAGTCAGG ACGGTAAGGC TTTGCTGGAC 
GTTCTTCAGC GGCCGTTGAG TCCTGGGAAC GCAGAGTCAC TGACTGCGAC AGCGAACTAC 
TCTAAAGCCG TGCACTGTAT ACTGGACGTG GTCCACGAGG TCCTGCATCA TCAGAGACGT 
CTGGAGAACA TCTGGCAGCA CCGTAAAGTC CGTCTGCATC AGAGACTGCA GCTCTGTGTC 
TTCCAGCAGG ACGTCCAACA GGTGATTGAC TGGATAGAGA ATCACGGGGA GGCTTTCCTC 
AGCAAACACA CTGGTGTGGG CAAATCCCTG CACAGAGCCA GAGCCCTCCA GAAACGCCAT 
GATGACTTTG AGGAGGTGGC ACAGAATACA TACACTAATG CAGATAAGCT GCTGGAAGCT 
GCTGAGCAGT TGGCTCAGAC GGGCGAGTGT GACCCTGAGG AGATCTACAA GGCAGCGCGA 
CACCTGGAAG TCAGGATCCA GGACTTTGTT AGGCGAGTGG AGCAAAGAAA ACTACTGCTG 
GACATGTCTG TCTCTTTCCA CACGCACACT AAAGAGTTGT GGACGTGGAT GGAGGACCTG 
CAGAAAGAGC TGCTGGAGGA AGTTTGTTCT GACTCAGTCG ATTCAGTTCA GGAGCTGATA 
AAACAGTTTC AGCAGCAGCA GACGGCCACG CTGGAGGCAA CACTCAATGT CATTAAAGAG 
GGAGAAGATC TTATGCAACA ACTCCGAGAT TCAGCAATGA GCAGTAATAA GATGCCCCAC 
ACCAGCTCCA TCGCTCACAT TGAGGGCGTC CTGCAGCAGC TAGAGGAGGC GCAGGGCCAC 
ATGGAGGAAC TCTTCCATGA GAGAAAGATC AAACTCGACA TCTTCCTGCA ACTGCGCATC 
TTTGAGCAGT ACACCATTGA GGTAACGGCA GAGTTAGACG CGTGGAATGA AGACTTGGTT 
CGTCAGATGA ATGAGTATAC TGGAGAAGAA CCCAGTCTGG CTGAGCAGAG ACTCCAGCGG 
CACGCAGAGA GGAAACTGGC CATGACTAAC ATGACCTACG AAGTTATGCA GCAGGGTCAG 
GACCTGCATC AGTACATCAT GGAAGTCCAA GCTTCAGGTA TTGAGTTGAC CGGAGAGAAG 
GAGGTGGATC TGGCAACACA AGTTCAGGAG CTGCTGGAGT TCCTTCATGA GAAACAGCAC 
GAGTTGGACA TGAGCGCCGA ACACACACAG AAGCGCCTGG AGCAGTGTGT GCAGCTCCGC 
CACCTGCAGG CTGAGGTTAA ACAGGTTCTG GGGTGGATTC GTAATGGTGA ATCTATGTTG 
GATGCCAGTA TGGTGAATGC AAGTTCACTG TCAGAGGCCG AACAACTACA GAGAGAACAT 
GAACAGTTCC AGCTGGCCAT AGAGTCTTTG TTCCACGCTA ACTCTCTCCA GAAAACCCAT 
CAGAGTGCAC TACAGGTCCA GCAGAAGGCA GAAATCATGT TACAGGCTGG ACACTTTGAC 
CCCGAGGCCA TCCGCAGCTG TGCAGAGAAG GTGGCCGTGC ACTGGCAGCA GCTCATGCTC 
AAGATGGAGG ACAGACTCAA ACTGGTTAAT GCCTCGGTGG CCTTCTACAA GACGTCAGAA 
CAGGTGTGTA GTGTCCTGGA GAGTCTGGAG CAGGAGTACC GCAGAGATGA AGACTGGTGT 
GGAGCTCATG ATAAACTAGG ATCTTCATCA GAGACTGATC ACGTTCTGCC TCTCATCAGC 
AAACACCTGG AGCAGAAAGA AGCTTTTCTC AAGGCATGCA CTCTGGCCAG GCGCAATGCT 
GAGGTTTTTC TGAAATACAT CCACCGGAAC AATGTCAGCT CTCAGGGATC ATCTAGCAAC 
ACTCGTGGCC CTGAGCAACA GGTCAAAGCC ATCCTGAATG AACTGCTGCA GAGGGAAAAC 
AGAGTTTTGC ATTTCTGGAC CATGAAGAAG CGGCGTTTGG ATCAGTGTCA GCAGTATGTG 
GTGTTTGAAC GCAATGCCAA ACAGGCATTA GAGTGGATTG AGGAGGCAGG AGAGGTCTAT 
CTGGCCACTC ACACCTGCAT CGGAGACTCT ATGGAGAAAA CTCAGGGACT CCTCAAAGAA 
TACGAAGAAT TCTGCATCTC CGCAAAGCAA ACTAAGGAGA AAGTGAAGAT GCTGATCCAG 
CTGGCGGATA ACTTTGTTGA GAAAGGACAC ATGCATGTCA CCGACTTGAA GAAGTGTCTG 
ACGGCAGTGG ACAAGCGCTA CAGGGACTTC TCCATGCGCA TGGGCAAATA CCGCTGTGAC 
CTGGAAAATG TGCTGGGCAT CAGCTCTGAG GATAATAAGG ATCTGGAGCT GGATATCATC 
CCAGCCAGTC TGACTGGCCC AGAGGTTAAA CTCCGTGACC CCAACCACGA GGTGAATGAA 
GAAAAGAGGA AATCAGCCCG GAAGAAAGAG TTTATTATAG CAGAGCTGTT GCAGACAGAA 
AAGGCTTATG TGAGGGACTT ACATGAATGC TTAGAGACAT ATTTGTGGGA GATGACCAAT 
GGTGTGGAGG AAATTCCTCC AGGAATCGCC AACAAAGAGC ACATCATCTT TGGCAACATT 
CAGGAGATTT ACGACTTCCA CAATAATATA TTCCTGAAGG AGCTGATCAA TTATGAGCAA 
CTGCCTGAGG ATGTGGGACA TTGCTTTGTG ACATGGGCCG ATAAGTTTAA CATTTATGTG 
ACATACTGCA AAAACAAACC AGATTCCAGT CAGCTCATCC TAGACCATGC TGGGAGTTTT 
TTTGATGAGA TTCAGAAGAG ACACAGTCTG ACCAACTCTA TATCATCATA CCTCATCAAA 
CCTGTACAGA GGATCACCAA ATATCAGCTG CTGCTCAAGG AGCTGCTGTC CTGCTGTGAA 
GAAGGCAAAG GTGAAATTAA AGATGGCCTG GAGGTCATGC TGAGTGTGCC AAAGAGAGCC 
AATGACGCCA TGCACGTCAG CATGCTGGAG GGTTTTGATG CAAATTTGGA TGTTCAGGGT 
GAACTCATCC TCCAAGACAC CTTCCAGGTG TGGGACCCCA AATCACTAAT CCGGAAGGGG 
CGAGACCGCC ACCTCTTTCT CTTCGAGATC TCCCTGGTCT TCAGTAAAGA GATCAAAGAC 
TCAGCAGGGC GATCCAAATA CATCTTTAAA AACAAACTAC TGACTTCTGA ATTGGGGGTG 
ACAGAGCATG TAGAGGGGGA CCCGTGCAAA TTTGCTCTGT GGGTGGGACG CACACCATCG 
TCTGACAACA AGACTGTCCT CAAGGCATCT AGTATTGAGG TGAAGCAAGA ATGGATTAAA 
AACATCCGAG AGGTCATTCA GGAGAGAATA ATTCACCTGA AGGGGGCGCT AAAGGAACCT 
ATCCACATTC CCAAAACTCC AGCCAAACCT CGCAATAACA GCAAGAGGGA CGCTGGTGAG 
GATGCTGACA GCCAGGGAGA CGGCAGCAGT CAGCCGGACA CCATCTCCAT AGCATCCAGA 
ACCTCTCAGA ACACTGTAGA CAGTGACAAG CTCTCTGGAG GGTGTGAGCT GACGGTGGTC 
CTGCAGGACT TTACAGCAAG CAGCTCATCA GAGCTCAGCA TCCAGACGGG TCAGACAGTG 
GAGCTGCTGG AGCGGCCCAG TGACCGTCCA GGATGGTGTC TGGTACGGAC CACCGAAAAG 
ACGCCTCCTC AAGAGGGACT GGTGCCCAGC AGTGCTCTGT GTATCTCGCA CTCACGCAGC 
AGTGTGGAGA TGGACTGCTT TTTCCCAGCA GGCAAAGAAA ACTATACAGT TAGTGGCCCA 
GAAGGAAAGA CAGAGTCAGT GGCCACACTG CAGCCACAGC CATCCCTGAA CTCTCTGCAG 
AGTAGCTCTC CTGGACCCAA GCGCTCTGGG AATACACTGA AGAAATGGCT GACCAGTCCT 
GTCCGACGTC TAAGTCATGG CAGTAACATC AAGAAGATCC CAAGTAAACA GAAACAGAAA 
CGTGATGGTC GCAAGAGTAT TGATATGGGG CCGCCCGAAC TACAGGATGA CACCTTGGAA 
GAGCGAGTGC AAAAAGAAGA GGGCACACTC TCCAAGTCTT CGTCTGGGAT GCACAGTGGA 
GGAGAGGAAG AGCCTGAGGA CGAGTCTCAC ACTCCTCTAC CTCCACCAAT GGAGATCATT 
AAAGACCCGT CGGCTCAGGA AGAGAAGACA CTGGAGCCAG GCTCATACCC GGGATTCAGC 
TCAGTCAATC CAGCAGAGCA GAGCCCGATT CCACCCAGAA ACCCAGAACT AGAGCAGAAA 
GCCAAAGCGC TACGAGGACG AATGTTTGTA GTCAAGGAAT TAATCCAAAC GGAAAAAGAC 
TATGTTAAAG ATCTGGGAAT TGTGGTTGAG GGGTTTATGA AGAGAATCGA GGAGAAAGGA 
GTTCCTGATA ACATGAAAGG AAAAGATAAG ATCGTCTTCG GAAACATTCA CCAAATCTAT 
GACTGGCACA AAGATTTTTT TGTTGGAGAA CTGGAGAAAT GCCTGGAAGA TCACGAAAAA 
CTGCCTGAAC TGTTCTGTAA ACATGAGAGA CGGTTACACA TGTATGTAGT TTACTGTCAG 
AACAAACCCA AATCCGAGTT CATCGTTGCA GAATATGATT CATACTTTGA GGGGATTCAG 
CAAGACATTA ACTCCAGACT GAGCATCAGC GATTTTCTCA TTAAACCCAT TCAGAGAATT 
ACTAAATACC AGCTTTTATT AAAGGATGTT TTGAAGTACA GTACAAAAGC AGGACTGGAT 
TGTCAGGATA TAGAGAATGC AGTTGACCTG ATGTCCCAGG TTCCCAAGCT TTGCAATGAC 
ATGATGAATC TGGGAAGACT GCAGGGATAT GAGGGGAAGT TGACGAGTCA AGGAAAACTC 
TTGCAGCAGG AGACGTTCTT TGTGACCGAG CAGGACTCTG GCGTTCTGTC CCGCAGTAAA 
GAGCGCAGGG TTTTCCTGTT CGAACAAATT GTCATCTTCA GCGAGCTGTT ACGTAAAGGC 
TCTTCCACTC CAGGGTACCA GTTCAAGAAG AGCATAAAGG TGAGCTTCCT TGACTTGGAA 
GAGCATGTTG AAAATGATCC ATGCAAGTTT GTGCTGTCGT GTCGTGGGTC GGCAGAACGT 
TTTACCCTTC AAGCAGCAAA CACGGACATT AAACAAGTCT GGGTTCAGCA TATCACCCAA 
CTACTAGATT TACAAAGCAA CTTCTTGTCA GCTCTGCAGT CTCCTATTGA GTATCAGAAG 
GAGCGTAGTG GCTCTCAATC TCTGACCCGG AACTCTTCTG GGCGGATGGG TCAGTCCAAC 
AGCAGGCCCT GCTCCACGGT TTCACTCGGA GCAGAGAAAC CCTCTCTGTC TGGACGGAGC 
TCCACCCCGC TAAAGCTATC TACCTCCAAT GGTGGTCCAT GCTCTGAAAG ATACCGCAGG 
CAGTTTGAAG GCTTGGGCTG CAACGGTACA TCGTCCTCCT TGATGGTGAC TCAAGATTAT 
ACTGCACTGA AAGAGAATGA GATCTGTGTC ACACAGGGTG AGGCCGTGCA GGTACTGGCC 
ACTAACCAGC AGAACATGTA CCTTGTGTAC CGACCCGCAA ATAGCCAATC ACCACCTGCA 
GAGGGCTGGG TGCCGGGACA CATCCTAGGC CCCGTCACAA AACCTCTCAT AGACACTACT 
GCAGACGCAA GCATCAAGAA ATCTCTTTCG TGGAACACAC TTCGTGCTCG TAAGCGTGCA 
GAAAAGGACA GCACGCCCCT CAAAAGTGAC TTTAAAGTTG AGAATGGACT GCGTAAGCCA 
AAGGAAATTC TCAGCAGCAA AGTCAAAGTG AAAGAGTCCC GCAGTTCTGA TGAGTCTGAA 
TGTGAGGATG AACTAGACCC CAAAACTAGT ATGGAGTTAC TGAACCCTAA TTTCATCCAA 
GAGGTCGCTC CAGAGTTTCT GACGCCTCTA ACAGACGTGA CGTGTGCTCT GGGAGAGACG 
GTGGTTCTGT GCTGTAAAGT GTGTGCTCGA CCCAAACCCA CAATCACCTT GAAAGGACCT 
GATCAAAGTC TGCTGGCCAA CAATAACCGC TTCACTATTA ATATCAGAGA AACCGGTGAT 
ATAGTGTTGA AAATCTGCAA TCTAATGCCG CAGGACACTG GCATCTACAC GTGTGTTGCT 
GTGAATGACC ATGGTACAGC CTCCTCATCT GCATCCATTA AAGTACAAGG TATCCCAGCA 
GCCCCTGCTC GACCGGTGGC ACAGGAGGCA AGCAGCACTG CAGTGATTGT CCACTGGCTT 
CCTCCAGCCA GCTCAGCAAA TTCTGCCATC TCCAGCTACA CTGTGGAATA TAGGCAGGAA 
GATTCTCTGG TATGGCAGCA GTTGGTGGTC TCCACGGCAG ATGTGTTTGT GAAGATTGAC 
AATCTGATTC CTGGTGGGCA CTACCAGTTC AGAGTCAGTG CCAGTAATCC CTGGGGCACC 
AGCCCGCCTA GTGAACCCTC AAACATGGTG ACATTATCCA GCACAGCATC AACATATGAT 
GGCGCTGGAA TCCAGTGGAA AGACAACTTT GAATCTGCTT TTACAGAATT ATGTGAAATT 
GGGCGGGGTC GTTTCTCTGT GGTGAGGAAG TGTCTCAGTA AAGCCACTAA GCAGGAGGTT 
GCTGTGAAGT TTGTGAATAA GAAGATGCAC AAGAAAGAAC AGGTGGCCCA TGAAGCTGAT 
ATTCTACGCC ATGTCCAGCA CCCCCAACTG GTGTCACTGA TTGATACATA CGAGTCGCCT 
ACAGCCTACA TGCTGATTCT TGAACTTGTT GAGGATGGAC GTCTTCTTGA CTATCTGGTA 
GCTCATGATG AGCTGATGGA GGAGAAAGTG GCATTCTTTA TTAAAGATAC ACTGGAAGCT 
CTGCAGCACT TGCACACCTG CAGAGTGGCT CATCTAGACC TCAAGCCTGA AAACTTGTTG 
GTGGACCTCC ACGTTCCGGT ACCCTGCGTG AAGCTCTCAG ATCTAGGTGA TGCTGTACAG 
GTTTCAAGTC ACCGCTACGT ACATCTCCTC CTGGGTAACC CCGAGTTCGC TGCTCCAGAG 
CTCATCCAGG GCACTCCAGT GTCCCTCTCT ACAGATGTGT GGAGTGTAGG GGTGCTGGCC 
TACGTCATGC TTAGTGGAGT CTCCCCATTC CTGGATGAGA GTCTGGAGGA GACCTGCGTC 
AACATCTGCA GGCTGGACTT CTGCTTTCCT CAAGAGTACT TCTCCGATAT AAGCCAGGCA 
GCCAAAGACT TCATTGTGTC CACGCTTAAC CAGGATCCCA GGAAGAGGCC AAGCTCGGCC 
TCGTGTCTTC AGCACCCATG GGTCAGTGCT CATAGTGGAG ATTACTCCAA AACACCTCTG 
GATACTGTCA GACTCGCTGC ATTTATAGAC AGACGCAAAC AGCTACATGA TGTCAGGCCT 
GTCACAAACA TTAAAGGAAT GGTCAGCAGC AGTATGGGAC ACACCTTATG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Gene Symbol	kalrna
Entrez Gene ID	100148988
Full Name	kalirin RhoGEF kinase a
Synonyms	si:dkey-275m12.1
General protein information	Preferred Names kalirin RhoGEF kinase a Names kalirin
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

Danio rerio (zebrafish)	LOC100148988	XP_003199221.2
Bos taurus (cattle)	LOC100847188	XP_005201508.1

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

kalrna cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

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