dgkzb cDNA ORF clone, Danio rerio(zebrafish)

The following dgkzb gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the dgkzb cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1+/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID Accession No. Definition **Vector *Turnaround time Price (USD) Select
ODa141418 XM_021470621.1
Latest version!
Danio rerio diacylglycerol kinase, zeta b (dgkzb), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 19-21 $713.30
$1019.00
ODa29152 XM_003201397.5
Latest version!
Danio rerio diacylglycerol kinase, zeta b (dgkzb), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa29153 XM_005174288.4
Latest version!
Danio rerio diacylglycerol kinase, zeta b (dgkzb), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa29154 XM_005174289.4
Latest version!
Danio rerio diacylglycerol kinase, zeta b (dgkzb), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa29155 XM_005174290.4
Latest version!
Danio rerio diacylglycerol kinase, zeta b (dgkzb), transcript variant X5, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
View Old Accession Versions >>
ODa29155 XM_005174290.3 Danio rerio diacylglycerol kinase, zeta b (dgkzb), transcript variant X4, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $629.30
$899.00
ODa29152 XM_003201397.4 Danio rerio diacylglycerol kinase, zeta b (dgkzb), transcript variant X1, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa29154 XM_005174289.3 Danio rerio diacylglycerol kinase, zeta b (dgkzb), transcript variant X3, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
ODa29153 XM_005174288.3 Danio rerio diacylglycerol kinase, zeta b (dgkzb), transcript variant X2, mRNA. pcDNA3.1-C-(k)DYK or customized vector 20 $713.30
$1019.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

  • Reference Sequences (Refseq)
    CloneID ODa141418
    Clone ID Related Accession (Same CDS sequence) XM_021470621.1
    Accession Version XM_021470621.1 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3624bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product diacylglycerol kinase zeta isoform X4
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGACACTT TTTTCCGACG GCATTTTAAG AGAAAGGAAG CAGGGGACGG TGTGCATGAT 
    GACTCCTGTC GACACCGGAG ACCGAGCATT GCAGTGCCAA CCAGCCGTGC CCGACGGCGC
    TCCAGTGTCG GCCTCCCATC CAGTGCATTA ACCCAGCGGC GCCGATCCAG CGTCCAACTT
    CAGGCATTGC CCCGAATCAA CAGCAGTCAA CCGGTACATC AAAAGAGCAG CGGTAGACGG
    AGGTCAAGTA CCACCACCCT GAAAGCCAAT CCTCGCTTTG CTGTGCGCCG TAAGAAAGTA
    GGCAAGCTTC GCAATATAGA CACACACCTC CTAGGCCCAT CCATGCTGCT AGCCAGTCTG
    ATCCAAATGA CTCAAGAAGA GGAAGACGAT GAAGAAGGTC GCAGTCCGCT CCCAGGGCAA
    GTGGAGCTCA AGGGAAGCAG CTCGAAGTTC AGGAGTCACA CAGATGTGCT GCTTTCTGAA
    GGTGACAGTG ATAGTGATGA CTCCAGCGAG CATTCAGAGG AACGTCAATA CTCAAGGATA
    CGCAAAGAGT GTCTGGAGGA GGCTGATGGG TCAGACTGGG GGGTCAAGGA GTCTGGTGTT
    TTATGCCAGC AGGACTGCCT TCCTCTGTCT GTGGTGGCGG AGCGAATCCA GGCCCATCCG
    CTGATTCGAG TCCCGCGCTG CCTCAGGAGG AACTCGTCCC ATTATGGCCA TGCGGATCCA
    GGACCGCATG TGCGCTACTG CCGCAGCAGC CAAAGGAGGC GTCGTAGGAG GGTCTCCACA
    ATCTCCAAAG CAGGAAGCCC ATGGCCTGGA AGAGGCCTGC CGCTGCCTAA CCGAAGAAGC
    TCCTGTTACA GGGGTCCGGC GACTTTCAAT CCTCTCCTGG GGGCTTATTA TGACCCCCGA
    TTGGAGTCCT GGAGTGGATT CTTGTCGAAA GCCATCGCCA AAGCTGGTGT GCAGGATAAC
    GCTGCTCAAT CCGGCACAAT AACACCTGCT AAAAGTGAAC CCAACAAAAA GATATGCAAC
    ACCGTGGATT GGACCGAAAA TGCCCAGTTG GGTCAACACG TGTGGTTTGA GACCAGTCCG
    GCAGGAGATT TCTGTTATGT GGGGGAGTCA AACTGTATCG CAAAGTCCCA GCAAAAGTCT
    CTTCCCCGCC ACAAATGTGC AGCTTGCAAA ATATCAGTTC ACACAGAGTG CATGAATCAG
    CTGGACAAGA TTAACTTCAG GTGTAAGCCA ACTTTTAAAG ATCCAGGGAC GAAAAATGTC
    AGAGAAACTC CAAATATGAA CCATCACTGG GTCCATAGGA GAAGGCAAGA TGGAAAATGC
    CGGCAATGTG GAAAGGGTTT CCAGCAGAAA TTCTCCTTCC ACAGTAAAGA CATTGTGGCC
    ATCAGTTGCT CATGGTGTAA ACAGGCTTAT CACAACAAGG TGAACTGTTT CATGATGCAG
    AGAATCGATG AGCCGTGTTC CCTTGGCGCT CACGCAGCTG TGATCATCCC TCCAACCTGG
    ATTATCAGAA TGCGGAGGCA GCAGGCATCA CTGAAGAGCA GTAAAAGAAG GAAGAGAACG
    TCTCTGAAGG CCAAACCCAG CAAGAAATTA GAGCAGGACG GCAAATGGAG AAACTTCGTG
    GTGAGAGCGA TTCCTTCCCA GCACATGAAG CCTCTGCTGG TGTTCGTCAA TCCGAAGAGT
    GGTGGAAACC AGGGAACGAA AATCATCCGT ACTTTCATGT GGTACCTGAA CCCCCGGCAA
    GTGTTCGACC TGACTCAGGG AGGTCCGAGA GAGGGGTTAG AAATGTACAG CAAAGTGCCC
    AACCTGCGCA TCCTTGTTTG TGGTGGCGAT GGAACGGTTG GCTGGATTCT GTCAGTGCTG
    GATGAGCTTC AGTTGAACCC TCAACCTGCT GTGGCCGTTC TTCCCCTTGG GACAGGAAAT
    GACCTGGCCA GGACCCTCAA CTGGGGCGGG GGCTACACAG ATGAACCAGT ATCCAAGATC
    CTTTCCCATG TTGAAGACGG CATCATCGTC CAGCTGGATC GCTGGAACCT GAGTGTGGAA
    GCTAATTTAG AGGCCAGCGA TGAGGACAAA GATGAACAAC AGACCGACAA GCTTCCCATT
    GACGTCTTCA ACAATTACTT CAGCCTCGGG TTTGACGCAC ATGTGACTTT GGAGTTTCAC
    GAATCCCGAG AGGCTAAACC GGAACGCTTC AACAGTCGAC TTCGCAATAA GATGTTTTAC
    GCAGGGACAG CATTCTCAGA CTTCCTGATG GGCAGCTCTA AAGATCTGTC CAAACACATC
    AAGGTTGTGT GTGACGGTAC TGACCTCACC AGTAAAATTC AAGATATGAA AGTGCTGTGC
    CTGCTGTTCC TCAACATCCC CAGATATTGC GCTGGTACCA TGCCATGGGG GAACCCAAGT
    GAGAACAATG ACTTCGGGCC ACAGAAACAC GACGACGGGC TTATTGAGGT CATCGGCTTC
    ACCATGACCT CTATGGCAAC TCTTCAGGTT GGAGGACATG GCGAGAGGCT TCATCAGTGT
    AAGGAGGTGA CCCTGACTAC TTTTAAGCCC ATTCCTGTAC AGGTGGATGG GGAACCCTGT
    AGACTGGCTC CATCCGTCAT ACACATCACT CGAAGGAACC AAGCCAACAT GCTGCAGAAA
    ACCAAGAGGA GGATCTCTCT ACCACAACTG TATGATCAGC AGCCCATCGC AGAGACAGTG
    CAGATCCAAG TGAACCGTAT CAGTATGCAT GATTATGAAG CTCTCCATTA TGATAAGGAG
    CAGCTCAAAG AAGCCGCGAT TCCCATGGGG ATAATTACAG TTCCTGGGAA CAGTGATTTG
    GAGACGTGTC GGCAGCATAT CGAGAGGTTG CATGAGGAGG GGGATGGGAT GAATACAGAC
    ACACTGCACA TGCCAAAGCT TTCACCAAAA TGGTGCTTCC TGGACTGTAC AACTGCAGAT
    CGTTTCTACC GAATAGATCG TGGTCAGGAG CATCTAAACT ATGTGTCGGA GATCTCCCAG
    GATGAGCTTT TCATCTTGGA CCCGGAGCTG GTCACCAAGG AGACGGTGGG CACGTCGCCC
    GGAATGCCGG GGGCGATGGA AACGGTTGTT GGGGGATGTG GATGTTCCAA CAGCACAGAC
    CAGTTCGCTT TTCCTGCCTG CTCTCCAGGG CCTTCTCCAC TCTGCAGGTT AGACACGGTC
    TGGAATAAGA GTGATCAAGA TTCAACCAAT TTGCTCTTTG ATGCTGTGAA GTCTGGCAAC
    TTGGAAAAGT TGGAACAGAT GCATGAAGAA GGAGCGGATC TCTCGGTCCA GGACCATTTA
    GGTTGCACCA TCCTCCATCA GGCAGTCATT TTAGGTCACA AAGAGATTGT GAAGTTTATC
    ACTGAAAACG CTCCTGCCTG CATACTGGAT GTGACGGAGA AATCAACAGG AGAGACAGCT
    CTGCATAAGG CAGCTGCGTT GTGCCATAGA ACAATTTGCT GTTATCTGGT GGATGCTGGT
    GCATCGCTCA TGAAGACAGA TCTACAGGGT GAAACTCCAA AGATGTACGC AGAGAGAACT
    CGAGACTACG AGCTAGCTGA ATATTTGGAA AACCAACAAC ACCATCAAAT GATCCAGAGA
    GATGACCATG AGACGGCAGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_021326296.1
    CDS309..3932
    Translation

    Target ORF information:

    RefSeq Version XM_021470621.1
    Organism Danio rerio(zebrafish)
    Definition Danio rerio diacylglycerol kinase, zeta b (dgkzb), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_021470621.1

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    ATGGACACTT TTTTCCGACG GCATTTTAAG AGAAAGGAAG CAGGGGACGG TGTGCATGAT 
    GACTCCTGTC GACACCGGAG ACCGAGCATT GCAGTGCCAA CCAGCCGTGC CCGACGGCGC
    TCCAGTGTCG GCCTCCCATC CAGTGCATTA ACCCAGCGGC GCCGATCCAG CGTCCAACTT
    CAGGCATTGC CCCGAATCAA CAGCAGTCAA CCGGTACATC AAAAGAGCAG CGGTAGACGG
    AGGTCAAGTA CCACCACCCT GAAAGCCAAT CCTCGCTTTG CTGTGCGCCG TAAGAAAGTA
    GGCAAGCTTC GCAATATAGA CACACACCTC CTAGGCCCAT CCATGCTGCT AGCCAGTCTG
    ATCCAAATGA CTCAAGAAGA GGAAGACGAT GAAGAAGGTC GCAGTCCGCT CCCAGGGCAA
    GTGGAGCTCA AGGGAAGCAG CTCGAAGTTC AGGAGTCACA CAGATGTGCT GCTTTCTGAA
    GGTGACAGTG ATAGTGATGA CTCCAGCGAG CATTCAGAGG AACGTCAATA CTCAAGGATA
    CGCAAAGAGT GTCTGGAGGA GGCTGATGGG TCAGACTGGG GGGTCAAGGA GTCTGGTGTT
    TTATGCCAGC AGGACTGCCT TCCTCTGTCT GTGGTGGCGG AGCGAATCCA GGCCCATCCG
    CTGATTCGAG TCCCGCGCTG CCTCAGGAGG AACTCGTCCC ATTATGGCCA TGCGGATCCA
    GGACCGCATG TGCGCTACTG CCGCAGCAGC CAAAGGAGGC GTCGTAGGAG GGTCTCCACA
    ATCTCCAAAG CAGGAAGCCC ATGGCCTGGA AGAGGCCTGC CGCTGCCTAA CCGAAGAAGC
    TCCTGTTACA GGGGTCCGGC GACTTTCAAT CCTCTCCTGG GGGCTTATTA TGACCCCCGA
    TTGGAGTCCT GGAGTGGATT CTTGTCGAAA GCCATCGCCA AAGCTGGTGT GCAGGATAAC
    GCTGCTCAAT CCGGCACAAT AACACCTGCT AAAAGTGAAC CCAACAAAAA GATATGCAAC
    ACCGTGGATT GGACCGAAAA TGCCCAGTTG GGTCAACACG TGTGGTTTGA GACCAGTCCG
    GCAGGAGATT TCTGTTATGT GGGGGAGTCA AACTGTATCG CAAAGTCCCA GCAAAAGTCT
    CTTCCCCGCC ACAAATGTGC AGCTTGCAAA ATATCAGTTC ACACAGAGTG CATGAATCAG
    CTGGACAAGA TTAACTTCAG GTGTAAGCCA ACTTTTAAAG ATCCAGGGAC GAAAAATGTC
    AGAGAAACTC CAAATATGAA CCATCACTGG GTCCATAGGA GAAGGCAAGA TGGAAAATGC
    CGGCAATGTG GAAAGGGTTT CCAGCAGAAA TTCTCCTTCC ACAGTAAAGA CATTGTGGCC
    ATCAGTTGCT CATGGTGTAA ACAGGCTTAT CACAACAAGG TGAACTGTTT CATGATGCAG
    AGAATCGATG AGCCGTGTTC CCTTGGCGCT CACGCAGCTG TGATCATCCC TCCAACCTGG
    ATTATCAGAA TGCGGAGGCA GCAGGCATCA CTGAAGAGCA GTAAAAGAAG GAAGAGAACG
    TCTCTGAAGG CCAAACCCAG CAAGAAATTA GAGCAGGACG GCAAATGGAG AAACTTCGTG
    GTGAGAGCGA TTCCTTCCCA GCACATGAAG CCTCTGCTGG TGTTCGTCAA TCCGAAGAGT
    GGTGGAAACC AGGGAACGAA AATCATCCGT ACTTTCATGT GGTACCTGAA CCCCCGGCAA
    GTGTTCGACC TGACTCAGGG AGGTCCGAGA GAGGGGTTAG AAATGTACAG CAAAGTGCCC
    AACCTGCGCA TCCTTGTTTG TGGTGGCGAT GGAACGGTTG GCTGGATTCT GTCAGTGCTG
    GATGAGCTTC AGTTGAACCC TCAACCTGCT GTGGCCGTTC TTCCCCTTGG GACAGGAAAT
    GACCTGGCCA GGACCCTCAA CTGGGGCGGG GGCTACACAG ATGAACCAGT ATCCAAGATC
    CTTTCCCATG TTGAAGACGG CATCATCGTC CAGCTGGATC GCTGGAACCT GAGTGTGGAA
    GCTAATTTAG AGGCCAGCGA TGAGGACAAA GATGAACAAC AGACCGACAA GCTTCCCATT
    GACGTCTTCA ACAATTACTT CAGCCTCGGG TTTGACGCAC ATGTGACTTT GGAGTTTCAC
    GAATCCCGAG AGGCTAAACC GGAACGCTTC AACAGTCGAC TTCGCAATAA GATGTTTTAC
    GCAGGGACAG CATTCTCAGA CTTCCTGATG GGCAGCTCTA AAGATCTGTC CAAACACATC
    AAGGTTGTGT GTGACGGTAC TGACCTCACC AGTAAAATTC AAGATATGAA AGTGCTGTGC
    CTGCTGTTCC TCAACATCCC CAGATATTGC GCTGGTACCA TGCCATGGGG GAACCCAAGT
    GAGAACAATG ACTTCGGGCC ACAGAAACAC GACGACGGGC TTATTGAGGT CATCGGCTTC
    ACCATGACCT CTATGGCAAC TCTTCAGGTT GGAGGACATG GCGAGAGGCT TCATCAGTGT
    AAGGAGGTGA CCCTGACTAC TTTTAAGCCC ATTCCTGTAC AGGTGGATGG GGAACCCTGT
    AGACTGGCTC CATCCGTCAT ACACATCACT CGAAGGAACC AAGCCAACAT GCTGCAGAAA
    ACCAAGAGGA GGATCTCTCT ACCACAACTG TATGATCAGC AGCCCATCGC AGAGACAGTG
    CAGATCCAAG TGAACCGTAT CAGTATGCAT GATTATGAAG CTCTCCATTA TGATAAGGAG
    CAGCTCAAAG AAGCCGCGAT TCCCATGGGG ATAATTACAG TTCCTGGGAA CAGTGATTTG
    GAGACGTGTC GGCAGCATAT CGAGAGGTTG CATGAGGAGG GGGATGGGAT GAATACAGAC
    ACACTGCACA TGCCAAAGCT TTCACCAAAA TGGTGCTTCC TGGACTGTAC AACTGCAGAT
    CGTTTCTACC GAATAGATCG TGGTCAGGAG CATCTAAACT ATGTGTCGGA GATCTCCCAG
    GATGAGCTTT TCATCTTGGA CCCGGAGCTG GTCACCAAGG AGACGGTGGG CACGTCGCCC
    GGAATGCCGG GGGCGATGGA AACGGTTGTT GGGGGATGTG GATGTTCCAA CAGCACAGAC
    CAGTTCGCTT TTCCTGCCTG CTCTCCAGGG CCTTCTCCAC TCTGCAGGTT AGACACGGTC
    TGGAATAAGA GTGATCAAGA TTCAACCAAT TTGCTCTTTG ATGCTGTGAA GTCTGGCAAC
    TTGGAAAAGT TGGAACAGAT GCATGAAGAA GGAGCGGATC TCTCGGTCCA GGACCATTTA
    GGTTGCACCA TCCTCCATCA GGCAGTCATT TTAGGTCACA AAGAGATTGT GAAGTTTATC
    ACTGAAAACG CTCCTGCCTG CATACTGGAT GTGACGGAGA AATCAACAGG AGAGACAGCT
    CTGCATAAGG CAGCTGCGTT GTGCCATAGA ACAATTTGCT GTTATCTGGT GGATGCTGGT
    GCATCGCTCA TGAAGACAGA TCTACAGGGT GAAACTCCAA AGATGTACGC AGAGAGAACT
    CGAGACTACG AGCTAGCTGA ATATTTGGAA AACCAACAAC ACCATCAAAT GATCCAGAGA
    GATGACCATG AGACGGCAGT GTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa29152
    Clone ID Related Accession (Same CDS sequence) XM_003201397.5 , XM_003201397.4
    Accession Version XM_003201397.5 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3903bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product diacylglycerol kinase zeta isoform X1
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_003201397.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGACACTT TTTTCCGACG GCATTTTAAG AGAAAGGAAG CAGGGGACGG TGTGCATGAT 
    GACTCCTGTC GACACCGGAG ACCGAGCATT GCAGTGCCAA CCAGCCGTGC CCGACGGCGC
    TCCAGTGTCG GCCTCCCATC CAGTGCATTA ACCCAGCGGC GCCGATCCAG CGTCCAACTT
    CAGGCATTGC CCCGAATCAA CAGCAGTCAA CCGGTACATC AAAAGAGCAG CGGTAGACGG
    AGGTCAAGTA CCACCACCCT GAAAGCCAAT CCTCGCTTTG CTGTGCGCCG TAAGAAAGTA
    GGCAAGCTTC GCAATATAGA CACACACCTC CTAGGCCCAT CCATGCTGCT AGCCAGTCTG
    ATCCAAATGA CTCAAGAAGA GGAAGACGAT GAAGAAGGTC GCAGTCCGCT CCCAGGGCAA
    GTGGAGCTCA AGGGAAGCAG CTCGAAGTTC AGGAGTCACA CAGATGTGCT GCTTTCTGAA
    GGTGACAGTG ATAGTGATGA CTCCAGCGAG CATTCAGAGG AACGTCAATA CTCAAGGATA
    CGCAAAGAGT GTCTGGAGGA GGCTGATGGG TCAGACTGGG GGGTCAAGGA GTCTGGTGTT
    TTATGCCAGC AGGACTGCCT TCCTCTGTCT GTGGTGGCGG AGCGAATCCA GGCCCATCCG
    CTGATTCGAG TCCCGCGCTG CCTCAGGAGG AACTCGTCCC ATTATGGCCA TGCGGATCCA
    GGACCGCATG TGCGCTACTG CCGCAGCAGC CAAAGGAGGC GTCGTAGGAG GGTCTCCACA
    ATCTCCAAAG CAGGAAGCCC ATGGCCTGGA AGAGGCCTGC CGCTGCCTAA CCGAAGAAGC
    TCCTGTTACA GGGGTCCGGC GACTTTCAAT CCTCTCCTGG GGGCTTATTA TGACCCCCGA
    TTGGAGTCCT GGAGTGGATT CTTGTCGAAA GCCATCGCCA AAGCTGGTGT GCAGGATAAC
    GCTGCTCAAT CCGGCACAAT AACACCTGCT AAAAGTGAAC CCAACAAAAA GATATGCAAC
    ACCGTGGATT GGACCGAAAA TGCCCAGTTG GGTCAACACG TGTGGTTTGA GACCAGTCCG
    GCAGGAGATT TCTGTTATGT GGGGGAGTCA AACTGTATCG CAAAGTCCCA GCAAAAGTCT
    CTTCCCCGCC ACAAATGTGC AGCTTGCAAA ATATCAGTTC ACACAGAGTG CATGAATCAG
    CTGGACAAGA TTAACTTCAG GTGTAAGCCA ACTTTTAAAG ATCCAGGGAC GAAAAATGTC
    AGAGAAACTC CAAATATGAA CCATCACTGG GTCCATAGGA GAAGGCAAGA TGGAAAATGC
    CGGCAATGTG GAAAGGGTTT CCAGCAGAAA TTCTCCTTCC ACAGTAAAGA CATTGTGGCC
    ATCAGTTGCT CATGGTGTAA ACAGGCTTAT CACAACAAGG TGAACTGTTT CATGATGCAG
    AGAATCGATG AGCCGTGTTC CCTTGGCGCT CACGCAGCTG TGATCATCCC TCCAACCTGG
    ATTATCAGAA TGCGGAGGCA GCAGGCATCA CTGAAGAGCA GTAAAAGAAG GAAGAGAACG
    TCTCTGAAGG CCAAACCCAG CAAGAAATTA GAGCAGGACG GCAAATGGAG AAACTTCGTG
    GTGAGAGCGA TTCCTTCCCA GCACATGAAG CCTCTGCTGG TGTTCGTCAA TCCGAAGAGT
    GGTGGAAACC AGGGAACGAA AATCATCCGT ACTTTCATGT GGTACCTGAA CCCCCGGCAA
    GTGTTCGACC TGACTCAGGG AGGTCCGAGA GAGGGGTTAG AAATGTACAG CAAAGTGCCC
    AACCTGCGCA TCCTTGTTTG TGGTGGCGAT GGAACGGTTG GCTGGATTCT GTCAGTGCTG
    GATGAGCTTC AGTTGAACCC TCAACCTGCT GTGGCCGTTC TTCCCCTTGG GACAGGAAAT
    GACCTGGCCA GGACCCTCAA CTGGGGCGGG GGCTACACAG ATGAACCAGT ATCCAAGATC
    CTTTCCCATG TTGAAGACGG CATCATCGTC CAGCTGGATC GCTGGAACCT GAGTGTGGAA
    GCTAATTTAG AGGCCAGCGA TGAGGACAAA GATGAACAAC AGACCGACAA GCTTCCCATT
    GACGTCTTCA ACAATTACTT CAGCCTCGGG TTTGACGCAC ATGTGACTTT GGAGTTTCAC
    GAATCCCGAG AGGCTAAACC GGAACGCTTC AACAGTCGAC TTCGCAATAA GATGTTTTAC
    GCAGGGACAG CATTCTCAGA CTTCCTGATG GGCAGCTCTA AAGATCTGTC CAAACACATC
    AAGGTTGTGT GTGACGGTAC TGACCTCACC AGTAAAATTC AAGATATGAA AGTGCTGTGC
    CTGCTGTTCC TCAACATCCC CAGATATTGC GCTGGTACCA TGCCATGGGG GAACCCAAGT
    GAGAACAATG ACTTCGGGCC ACAGAAACAC GACGACGGGC TTATTGAGGT CATCGGCTTC
    ACCATGACCT CTATGGCAAC TCTTCAGGTT GGAGGACATG GCGAGAGGCT TCATCAGTGT
    AAGGAGGTGA CCCTGACTAC TTTTAAGCCC ATTCCTGTAC AGGTGGATGG GGAACCCTGT
    AGACTGGCTC CATCCGTCAT ACACATCACT CGAAGGAACC AAGCCAACAT GCTGCAGAAA
    ACCAAGAGGA GGATCTCTCT ACCACAACTG TATGATCAGC AGCCCATCGC AGAGACAGTG
    CAGATCCAAG TGAACCGTAT CAGTATGCAT GATTATGAAG CTCTCCATTA TGATAAGGAG
    CAGCTCAAAG AAGCCGCGAT TCCCATGGGG ATAATTACAG TTCCTGGGAA CAGTGATTTG
    GAGACGTGTC GGCAGCATAT CGAGAGGTTG CATGAGGAGG GGGATGGGAT GAATACAGAC
    ACACTGCACA TGCCAAAGCT TTCACCAAAA TGGTGCTTCC TGGACTGTAC AACTGCAGAT
    CGTTTCTACC GAATAGATCG TGGTCAGGAG CATCTAAACT ATGTGTCGGA GATCTCCCAG
    GATGAGCTTT TCATCTTGGA CCCGGAGCTG GTCACCAAGG AGACGGTGGG CACGTCGCCC
    GGAATGCCGG GGGCGATGGA AACGGTTGTT GGGGGATGTG GATGTTCCAA CAGCACAGAC
    CAGTTCGCTT TTCCTGCCTG CTCTCCAGGG CCTTCTCCAC TCTGCAGGGT TAATGAAACA
    CATGCTGAAA GCCAACCGGA AAGTGTGAGT GAAGAAATCG ACATGCATGT GCAAAGTGAT
    CCAGAAAGTC CAACAAATCT AGGCGTGTAC AGGGTTCATG ATACACATGC TGAAAGCCAA
    CTGGAAAGCT TGAGTCCAGA AAGTGACTCT CATGTGCAAA GTGATCCAGG AAGTCCCACA
    AAATCAGGCC TGTACAGGAG GGGAGCAAAA AATAAGATTT CCAGTCGTTC AAACACACTT
    GAGGAGGACT CAGTGAATGT GCAGAGGTTA GACACGGTCT GGAATAAGAG TGATCAAGAT
    TCAACCAATT TGCTCTTTGA TGCTGTGAAG TCTGGCAACT TGGAAAAGTT GGAACAGATG
    CATGAAGAAG GAGCGGATCT CTCGGTCCAG GACCATTTAG GTTGCACCAT CCTCCATCAG
    GCAGTCATTT TAGGTCACAA AGAGATTGTG AAGTTTATCA CTGAAAACGC TCCTGCCTGC
    ATACTGGATG TGACGGAGAA ATCAACAGGA GAGACAGCTC TGCATAAGGC AGCTGCGTTG
    TGCCATAGAA CAATTTGCTG TTATCTGGTG GATGCTGGTG CATCGCTCAT GAAGACAGAT
    CTACAGGGTG AAACTCCAAA GATGTACGCA GAGAGAACTC GAGACTACGA GCTAGCTGAA
    TATTTGGAAA ACCAACAACA CCATCAAATG ATCCAGAGAG ATGACCATGA GACGGCAGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003201445.1
    CDS308..4210
    Translation

    Target ORF information:

    RefSeq Version XM_003201397.5
    Organism Danio rerio(zebrafish)
    Definition Danio rerio diacylglycerol kinase, zeta b (dgkzb), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003201397.5

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGACACTT TTTTCCGACG GCATTTTAAG AGAAAGGAAG CAGGGGACGG TGTGCATGAT 
    GACTCCTGTC GACACCGGAG ACCGAGCATT GCAGTGCCAA CCAGCCGTGC CCGACGGCGC
    TCCAGTGTCG GCCTCCCATC CAGTGCATTA ACCCAGCGGC GCCGATCCAG CGTCCAACTT
    CAGGCATTGC CCCGAATCAA CAGCAGTCAA CCGGTACATC AAAAGAGCAG CGGTAGACGG
    AGGTCAAGTA CCACCACCCT GAAAGCCAAT CCTCGCTTTG CTGTGCGCCG TAAGAAAGTA
    GGCAAGCTTC GCAATATAGA CACACACCTC CTAGGCCCAT CCATGCTGCT AGCCAGTCTG
    ATCCAAATGA CTCAAGAAGA GGAAGACGAT GAAGAAGGTC GCAGTCCGCT CCCAGGGCAA
    GTGGAGCTCA AGGGAAGCAG CTCGAAGTTC AGGAGTCACA CAGATGTGCT GCTTTCTGAA
    GGTGACAGTG ATAGTGATGA CTCCAGCGAG CATTCAGAGG AACGTCAATA CTCAAGGATA
    CGCAAAGAGT GTCTGGAGGA GGCTGATGGG TCAGACTGGG GGGTCAAGGA GTCTGGTGTT
    TTATGCCAGC AGGACTGCCT TCCTCTGTCT GTGGTGGCGG AGCGAATCCA GGCCCATCCG
    CTGATTCGAG TCCCGCGCTG CCTCAGGAGG AACTCGTCCC ATTATGGCCA TGCGGATCCA
    GGACCGCATG TGCGCTACTG CCGCAGCAGC CAAAGGAGGC GTCGTAGGAG GGTCTCCACA
    ATCTCCAAAG CAGGAAGCCC ATGGCCTGGA AGAGGCCTGC CGCTGCCTAA CCGAAGAAGC
    TCCTGTTACA GGGGTCCGGC GACTTTCAAT CCTCTCCTGG GGGCTTATTA TGACCCCCGA
    TTGGAGTCCT GGAGTGGATT CTTGTCGAAA GCCATCGCCA AAGCTGGTGT GCAGGATAAC
    GCTGCTCAAT CCGGCACAAT AACACCTGCT AAAAGTGAAC CCAACAAAAA GATATGCAAC
    ACCGTGGATT GGACCGAAAA TGCCCAGTTG GGTCAACACG TGTGGTTTGA GACCAGTCCG
    GCAGGAGATT TCTGTTATGT GGGGGAGTCA AACTGTATCG CAAAGTCCCA GCAAAAGTCT
    CTTCCCCGCC ACAAATGTGC AGCTTGCAAA ATATCAGTTC ACACAGAGTG CATGAATCAG
    CTGGACAAGA TTAACTTCAG GTGTAAGCCA ACTTTTAAAG ATCCAGGGAC GAAAAATGTC
    AGAGAAACTC CAAATATGAA CCATCACTGG GTCCATAGGA GAAGGCAAGA TGGAAAATGC
    CGGCAATGTG GAAAGGGTTT CCAGCAGAAA TTCTCCTTCC ACAGTAAAGA CATTGTGGCC
    ATCAGTTGCT CATGGTGTAA ACAGGCTTAT CACAACAAGG TGAACTGTTT CATGATGCAG
    AGAATCGATG AGCCGTGTTC CCTTGGCGCT CACGCAGCTG TGATCATCCC TCCAACCTGG
    ATTATCAGAA TGCGGAGGCA GCAGGCATCA CTGAAGAGCA GTAAAAGAAG GAAGAGAACG
    TCTCTGAAGG CCAAACCCAG CAAGAAATTA GAGCAGGACG GCAAATGGAG AAACTTCGTG
    GTGAGAGCGA TTCCTTCCCA GCACATGAAG CCTCTGCTGG TGTTCGTCAA TCCGAAGAGT
    GGTGGAAACC AGGGAACGAA AATCATCCGT ACTTTCATGT GGTACCTGAA CCCCCGGCAA
    GTGTTCGACC TGACTCAGGG AGGTCCGAGA GAGGGGTTAG AAATGTACAG CAAAGTGCCC
    AACCTGCGCA TCCTTGTTTG TGGTGGCGAT GGAACGGTTG GCTGGATTCT GTCAGTGCTG
    GATGAGCTTC AGTTGAACCC TCAACCTGCT GTGGCCGTTC TTCCCCTTGG GACAGGAAAT
    GACCTGGCCA GGACCCTCAA CTGGGGCGGG GGCTACACAG ATGAACCAGT ATCCAAGATC
    CTTTCCCATG TTGAAGACGG CATCATCGTC CAGCTGGATC GCTGGAACCT GAGTGTGGAA
    GCTAATTTAG AGGCCAGCGA TGAGGACAAA GATGAACAAC AGACCGACAA GCTTCCCATT
    GACGTCTTCA ACAATTACTT CAGCCTCGGG TTTGACGCAC ATGTGACTTT GGAGTTTCAC
    GAATCCCGAG AGGCTAAACC GGAACGCTTC AACAGTCGAC TTCGCAATAA GATGTTTTAC
    GCAGGGACAG CATTCTCAGA CTTCCTGATG GGCAGCTCTA AAGATCTGTC CAAACACATC
    AAGGTTGTGT GTGACGGTAC TGACCTCACC AGTAAAATTC AAGATATGAA AGTGCTGTGC
    CTGCTGTTCC TCAACATCCC CAGATATTGC GCTGGTACCA TGCCATGGGG GAACCCAAGT
    GAGAACAATG ACTTCGGGCC ACAGAAACAC GACGACGGGC TTATTGAGGT CATCGGCTTC
    ACCATGACCT CTATGGCAAC TCTTCAGGTT GGAGGACATG GCGAGAGGCT TCATCAGTGT
    AAGGAGGTGA CCCTGACTAC TTTTAAGCCC ATTCCTGTAC AGGTGGATGG GGAACCCTGT
    AGACTGGCTC CATCCGTCAT ACACATCACT CGAAGGAACC AAGCCAACAT GCTGCAGAAA
    ACCAAGAGGA GGATCTCTCT ACCACAACTG TATGATCAGC AGCCCATCGC AGAGACAGTG
    CAGATCCAAG TGAACCGTAT CAGTATGCAT GATTATGAAG CTCTCCATTA TGATAAGGAG
    CAGCTCAAAG AAGCCGCGAT TCCCATGGGG ATAATTACAG TTCCTGGGAA CAGTGATTTG
    GAGACGTGTC GGCAGCATAT CGAGAGGTTG CATGAGGAGG GGGATGGGAT GAATACAGAC
    ACACTGCACA TGCCAAAGCT TTCACCAAAA TGGTGCTTCC TGGACTGTAC AACTGCAGAT
    CGTTTCTACC GAATAGATCG TGGTCAGGAG CATCTAAACT ATGTGTCGGA GATCTCCCAG
    GATGAGCTTT TCATCTTGGA CCCGGAGCTG GTCACCAAGG AGACGGTGGG CACGTCGCCC
    GGAATGCCGG GGGCGATGGA AACGGTTGTT GGGGGATGTG GATGTTCCAA CAGCACAGAC
    CAGTTCGCTT TTCCTGCCTG CTCTCCAGGG CCTTCTCCAC TCTGCAGGGT TAATGAAACA
    CATGCTGAAA GCCAACCGGA AAGTGTGAGT GAAGAAATCG ACATGCATGT GCAAAGTGAT
    CCAGAAAGTC CAACAAATCT AGGCGTGTAC AGGGTTCATG ATACACATGC TGAAAGCCAA
    CTGGAAAGCT TGAGTCCAGA AAGTGACTCT CATGTGCAAA GTGATCCAGG AAGTCCCACA
    AAATCAGGCC TGTACAGGAG GGGAGCAAAA AATAAGATTT CCAGTCGTTC AAACACACTT
    GAGGAGGACT CAGTGAATGT GCAGAGGTTA GACACGGTCT GGAATAAGAG TGATCAAGAT
    TCAACCAATT TGCTCTTTGA TGCTGTGAAG TCTGGCAACT TGGAAAAGTT GGAACAGATG
    CATGAAGAAG GAGCGGATCT CTCGGTCCAG GACCATTTAG GTTGCACCAT CCTCCATCAG
    GCAGTCATTT TAGGTCACAA AGAGATTGTG AAGTTTATCA CTGAAAACGC TCCTGCCTGC
    ATACTGGATG TGACGGAGAA ATCAACAGGA GAGACAGCTC TGCATAAGGC AGCTGCGTTG
    TGCCATAGAA CAATTTGCTG TTATCTGGTG GATGCTGGTG CATCGCTCAT GAAGACAGAT
    CTACAGGGTG AAACTCCAAA GATGTACGCA GAGAGAACTC GAGACTACGA GCTAGCTGAA
    TATTTGGAAA ACCAACAACA CCATCAAATG ATCCAGAGAG ATGACCATGA GACGGCAGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa29152
    Clone ID Related Accession (Same CDS sequence) XM_003201397.5 , XM_003201397.4
    Accession Version XM_003201397.4 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3903bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product diacylglycerol kinase zeta isoform X1
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_003201397.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGACACTT TTTTCCGACG GCATTTTAAG AGAAAGGAAG CAGGGGACGG TGTGCATGAT 
    GACTCCTGTC GACACCGGAG ACCGAGCATT GCAGTGCCAA CCAGCCGTGC CCGACGGCGC
    TCCAGTGTCG GCCTCCCATC CAGTGCATTA ACCCAGCGGC GCCGATCCAG CGTCCAACTT
    CAGGCATTGC CCCGAATCAA CAGCAGTCAA CCGGTACATC AAAAGAGCAG CGGTAGACGG
    AGGTCAAGTA CCACCACCCT GAAAGCCAAT CCTCGCTTTG CTGTGCGCCG TAAGAAAGTA
    GGCAAGCTTC GCAATATAGA CACACACCTC CTAGGCCCAT CCATGCTGCT AGCCAGTCTG
    ATCCAAATGA CTCAAGAAGA GGAAGACGAT GAAGAAGGTC GCAGTCCGCT CCCAGGGCAA
    GTGGAGCTCA AGGGAAGCAG CTCGAAGTTC AGGAGTCACA CAGATGTGCT GCTTTCTGAA
    GGTGACAGTG ATAGTGATGA CTCCAGCGAG CATTCAGAGG AACGTCAATA CTCAAGGATA
    CGCAAAGAGT GTCTGGAGGA GGCTGATGGG TCAGACTGGG GGGTCAAGGA GTCTGGTGTT
    TTATGCCAGC AGGACTGCCT TCCTCTGTCT GTGGTGGCGG AGCGAATCCA GGCCCATCCG
    CTGATTCGAG TCCCGCGCTG CCTCAGGAGG AACTCGTCCC ATTATGGCCA TGCGGATCCA
    GGACCGCATG TGCGCTACTG CCGCAGCAGC CAAAGGAGGC GTCGTAGGAG GGTCTCCACA
    ATCTCCAAAG CAGGAAGCCC ATGGCCTGGA AGAGGCCTGC CGCTGCCTAA CCGAAGAAGC
    TCCTGTTACA GGGGTCCGGC GACTTTCAAT CCTCTCCTGG GGGCTTATTA TGACCCCCGA
    TTGGAGTCCT GGAGTGGATT CTTGTCGAAA GCCATCGCCA AAGCTGGTGT GCAGGATAAC
    GCTGCTCAAT CCGGCACAAT AACACCTGCT AAAAGTGAAC CCAACAAAAA GATATGCAAC
    ACCGTGGATT GGACCGAAAA TGCCCAGTTG GGTCAACACG TGTGGTTTGA GACCAGTCCG
    GCAGGAGATT TCTGTTATGT GGGGGAGTCA AACTGTATCG CAAAGTCCCA GCAAAAGTCT
    CTTCCCCGCC ACAAATGTGC AGCTTGCAAA ATATCAGTTC ACACAGAGTG CATGAATCAG
    CTGGACAAGA TTAACTTCAG GTGTAAGCCA ACTTTTAAAG ATCCAGGGAC GAAAAATGTC
    AGAGAAACTC CAAATATGAA CCATCACTGG GTCCATAGGA GAAGGCAAGA TGGAAAATGC
    CGGCAATGTG GAAAGGGTTT CCAGCAGAAA TTCTCCTTCC ACAGTAAAGA CATTGTGGCC
    ATCAGTTGCT CATGGTGTAA ACAGGCTTAT CACAACAAGG TGAACTGTTT CATGATGCAG
    AGAATCGATG AGCCGTGTTC CCTTGGCGCT CACGCAGCTG TGATCATCCC TCCAACCTGG
    ATTATCAGAA TGCGGAGGCA GCAGGCATCA CTGAAGAGCA GTAAAAGAAG GAAGAGAACG
    TCTCTGAAGG CCAAACCCAG CAAGAAATTA GAGCAGGACG GCAAATGGAG AAACTTCGTG
    GTGAGAGCGA TTCCTTCCCA GCACATGAAG CCTCTGCTGG TGTTCGTCAA TCCGAAGAGT
    GGTGGAAACC AGGGAACGAA AATCATCCGT ACTTTCATGT GGTACCTGAA CCCCCGGCAA
    GTGTTCGACC TGACTCAGGG AGGTCCGAGA GAGGGGTTAG AAATGTACAG CAAAGTGCCC
    AACCTGCGCA TCCTTGTTTG TGGTGGCGAT GGAACGGTTG GCTGGATTCT GTCAGTGCTG
    GATGAGCTTC AGTTGAACCC TCAACCTGCT GTGGCCGTTC TTCCCCTTGG GACAGGAAAT
    GACCTGGCCA GGACCCTCAA CTGGGGCGGG GGCTACACAG ATGAACCAGT ATCCAAGATC
    CTTTCCCATG TTGAAGACGG CATCATCGTC CAGCTGGATC GCTGGAACCT GAGTGTGGAA
    GCTAATTTAG AGGCCAGCGA TGAGGACAAA GATGAACAAC AGACCGACAA GCTTCCCATT
    GACGTCTTCA ACAATTACTT CAGCCTCGGG TTTGACGCAC ATGTGACTTT GGAGTTTCAC
    GAATCCCGAG AGGCTAAACC GGAACGCTTC AACAGTCGAC TTCGCAATAA GATGTTTTAC
    GCAGGGACAG CATTCTCAGA CTTCCTGATG GGCAGCTCTA AAGATCTGTC CAAACACATC
    AAGGTTGTGT GTGACGGTAC TGACCTCACC AGTAAAATTC AAGATATGAA AGTGCTGTGC
    CTGCTGTTCC TCAACATCCC CAGATATTGC GCTGGTACCA TGCCATGGGG GAACCCAAGT
    GAGAACAATG ACTTCGGGCC ACAGAAACAC GACGACGGGC TTATTGAGGT CATCGGCTTC
    ACCATGACCT CTATGGCAAC TCTTCAGGTT GGAGGACATG GCGAGAGGCT TCATCAGTGT
    AAGGAGGTGA CCCTGACTAC TTTTAAGCCC ATTCCTGTAC AGGTGGATGG GGAACCCTGT
    AGACTGGCTC CATCCGTCAT ACACATCACT CGAAGGAACC AAGCCAACAT GCTGCAGAAA
    ACCAAGAGGA GGATCTCTCT ACCACAACTG TATGATCAGC AGCCCATCGC AGAGACAGTG
    CAGATCCAAG TGAACCGTAT CAGTATGCAT GATTATGAAG CTCTCCATTA TGATAAGGAG
    CAGCTCAAAG AAGCCGCGAT TCCCATGGGG ATAATTACAG TTCCTGGGAA CAGTGATTTG
    GAGACGTGTC GGCAGCATAT CGAGAGGTTG CATGAGGAGG GGGATGGGAT GAATACAGAC
    ACACTGCACA TGCCAAAGCT TTCACCAAAA TGGTGCTTCC TGGACTGTAC AACTGCAGAT
    CGTTTCTACC GAATAGATCG TGGTCAGGAG CATCTAAACT ATGTGTCGGA GATCTCCCAG
    GATGAGCTTT TCATCTTGGA CCCGGAGCTG GTCACCAAGG AGACGGTGGG CACGTCGCCC
    GGAATGCCGG GGGCGATGGA AACGGTTGTT GGGGGATGTG GATGTTCCAA CAGCACAGAC
    CAGTTCGCTT TTCCTGCCTG CTCTCCAGGG CCTTCTCCAC TCTGCAGGGT TAATGAAACA
    CATGCTGAAA GCCAACCGGA AAGTGTGAGT GAAGAAATCG ACATGCATGT GCAAAGTGAT
    CCAGAAAGTC CAACAAATCT AGGCGTGTAC AGGGTTCATG ATACACATGC TGAAAGCCAA
    CTGGAAAGCT TGAGTCCAGA AAGTGACTCT CATGTGCAAA GTGATCCAGG AAGTCCCACA
    AAATCAGGCC TGTACAGGAG GGGAGCAAAA AATAAGATTT CCAGTCGTTC AAACACACTT
    GAGGAGGACT CAGTGAATGT GCAGAGGTTA GACACGGTCT GGAATAAGAG TGATCAAGAT
    TCAACCAATT TGCTCTTTGA TGCTGTGAAG TCTGGCAACT TGGAAAAGTT GGAACAGATG
    CATGAAGAAG GAGCGGATCT CTCGGTCCAG GACCATTTAG GTTGCACCAT CCTCCATCAG
    GCAGTCATTT TAGGTCACAA AGAGATTGTG AAGTTTATCA CTGAAAACGC TCCTGCCTGC
    ATACTGGATG TGACGGAGAA ATCAACAGGA GAGACAGCTC TGCATAAGGC AGCTGCGTTG
    TGCCATAGAA CAATTTGCTG TTATCTGGTG GATGCTGGTG CATCGCTCAT GAAGACAGAT
    CTACAGGGTG AAACTCCAAA GATGTACGCA GAGAGAACTC GAGACTACGA GCTAGCTGAA
    TATTTGGAAA ACCAACAACA CCATCAAATG ATCCAGAGAG ATGACCATGA GACGGCAGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_003201445.1
    CDS300..4202
    Translation

    Target ORF information:

    RefSeq Version XM_003201397.4
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio diacylglycerol kinase, zeta b (dgkzb), transcript variant X1, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_003201397.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    3901
    ATGGACACTT TTTTCCGACG GCATTTTAAG AGAAAGGAAG CAGGGGACGG TGTGCATGAT 
    GACTCCTGTC GACACCGGAG ACCGAGCATT GCAGTGCCAA CCAGCCGTGC CCGACGGCGC
    TCCAGTGTCG GCCTCCCATC CAGTGCATTA ACCCAGCGGC GCCGATCCAG CGTCCAACTT
    CAGGCATTGC CCCGAATCAA CAGCAGTCAA CCGGTACATC AAAAGAGCAG CGGTAGACGG
    AGGTCAAGTA CCACCACCCT GAAAGCCAAT CCTCGCTTTG CTGTGCGCCG TAAGAAAGTA
    GGCAAGCTTC GCAATATAGA CACACACCTC CTAGGCCCAT CCATGCTGCT AGCCAGTCTG
    ATCCAAATGA CTCAAGAAGA GGAAGACGAT GAAGAAGGTC GCAGTCCGCT CCCAGGGCAA
    GTGGAGCTCA AGGGAAGCAG CTCGAAGTTC AGGAGTCACA CAGATGTGCT GCTTTCTGAA
    GGTGACAGTG ATAGTGATGA CTCCAGCGAG CATTCAGAGG AACGTCAATA CTCAAGGATA
    CGCAAAGAGT GTCTGGAGGA GGCTGATGGG TCAGACTGGG GGGTCAAGGA GTCTGGTGTT
    TTATGCCAGC AGGACTGCCT TCCTCTGTCT GTGGTGGCGG AGCGAATCCA GGCCCATCCG
    CTGATTCGAG TCCCGCGCTG CCTCAGGAGG AACTCGTCCC ATTATGGCCA TGCGGATCCA
    GGACCGCATG TGCGCTACTG CCGCAGCAGC CAAAGGAGGC GTCGTAGGAG GGTCTCCACA
    ATCTCCAAAG CAGGAAGCCC ATGGCCTGGA AGAGGCCTGC CGCTGCCTAA CCGAAGAAGC
    TCCTGTTACA GGGGTCCGGC GACTTTCAAT CCTCTCCTGG GGGCTTATTA TGACCCCCGA
    TTGGAGTCCT GGAGTGGATT CTTGTCGAAA GCCATCGCCA AAGCTGGTGT GCAGGATAAC
    GCTGCTCAAT CCGGCACAAT AACACCTGCT AAAAGTGAAC CCAACAAAAA GATATGCAAC
    ACCGTGGATT GGACCGAAAA TGCCCAGTTG GGTCAACACG TGTGGTTTGA GACCAGTCCG
    GCAGGAGATT TCTGTTATGT GGGGGAGTCA AACTGTATCG CAAAGTCCCA GCAAAAGTCT
    CTTCCCCGCC ACAAATGTGC AGCTTGCAAA ATATCAGTTC ACACAGAGTG CATGAATCAG
    CTGGACAAGA TTAACTTCAG GTGTAAGCCA ACTTTTAAAG ATCCAGGGAC GAAAAATGTC
    AGAGAAACTC CAAATATGAA CCATCACTGG GTCCATAGGA GAAGGCAAGA TGGAAAATGC
    CGGCAATGTG GAAAGGGTTT CCAGCAGAAA TTCTCCTTCC ACAGTAAAGA CATTGTGGCC
    ATCAGTTGCT CATGGTGTAA ACAGGCTTAT CACAACAAGG TGAACTGTTT CATGATGCAG
    AGAATCGATG AGCCGTGTTC CCTTGGCGCT CACGCAGCTG TGATCATCCC TCCAACCTGG
    ATTATCAGAA TGCGGAGGCA GCAGGCATCA CTGAAGAGCA GTAAAAGAAG GAAGAGAACG
    TCTCTGAAGG CCAAACCCAG CAAGAAATTA GAGCAGGACG GCAAATGGAG AAACTTCGTG
    GTGAGAGCGA TTCCTTCCCA GCACATGAAG CCTCTGCTGG TGTTCGTCAA TCCGAAGAGT
    GGTGGAAACC AGGGAACGAA AATCATCCGT ACTTTCATGT GGTACCTGAA CCCCCGGCAA
    GTGTTCGACC TGACTCAGGG AGGTCCGAGA GAGGGGTTAG AAATGTACAG CAAAGTGCCC
    AACCTGCGCA TCCTTGTTTG TGGTGGCGAT GGAACGGTTG GCTGGATTCT GTCAGTGCTG
    GATGAGCTTC AGTTGAACCC TCAACCTGCT GTGGCCGTTC TTCCCCTTGG GACAGGAAAT
    GACCTGGCCA GGACCCTCAA CTGGGGCGGG GGCTACACAG ATGAACCAGT ATCCAAGATC
    CTTTCCCATG TTGAAGACGG CATCATCGTC CAGCTGGATC GCTGGAACCT GAGTGTGGAA
    GCTAATTTAG AGGCCAGCGA TGAGGACAAA GATGAACAAC AGACCGACAA GCTTCCCATT
    GACGTCTTCA ACAATTACTT CAGCCTCGGG TTTGACGCAC ATGTGACTTT GGAGTTTCAC
    GAATCCCGAG AGGCTAAACC GGAACGCTTC AACAGTCGAC TTCGCAATAA GATGTTTTAC
    GCAGGGACAG CATTCTCAGA CTTCCTGATG GGCAGCTCTA AAGATCTGTC CAAACACATC
    AAGGTTGTGT GTGACGGTAC TGACCTCACC AGTAAAATTC AAGATATGAA AGTGCTGTGC
    CTGCTGTTCC TCAACATCCC CAGATATTGC GCTGGTACCA TGCCATGGGG GAACCCAAGT
    GAGAACAATG ACTTCGGGCC ACAGAAACAC GACGACGGGC TTATTGAGGT CATCGGCTTC
    ACCATGACCT CTATGGCAAC TCTTCAGGTT GGAGGACATG GCGAGAGGCT TCATCAGTGT
    AAGGAGGTGA CCCTGACTAC TTTTAAGCCC ATTCCTGTAC AGGTGGATGG GGAACCCTGT
    AGACTGGCTC CATCCGTCAT ACACATCACT CGAAGGAACC AAGCCAACAT GCTGCAGAAA
    ACCAAGAGGA GGATCTCTCT ACCACAACTG TATGATCAGC AGCCCATCGC AGAGACAGTG
    CAGATCCAAG TGAACCGTAT CAGTATGCAT GATTATGAAG CTCTCCATTA TGATAAGGAG
    CAGCTCAAAG AAGCCGCGAT TCCCATGGGG ATAATTACAG TTCCTGGGAA CAGTGATTTG
    GAGACGTGTC GGCAGCATAT CGAGAGGTTG CATGAGGAGG GGGATGGGAT GAATACAGAC
    ACACTGCACA TGCCAAAGCT TTCACCAAAA TGGTGCTTCC TGGACTGTAC AACTGCAGAT
    CGTTTCTACC GAATAGATCG TGGTCAGGAG CATCTAAACT ATGTGTCGGA GATCTCCCAG
    GATGAGCTTT TCATCTTGGA CCCGGAGCTG GTCACCAAGG AGACGGTGGG CACGTCGCCC
    GGAATGCCGG GGGCGATGGA AACGGTTGTT GGGGGATGTG GATGTTCCAA CAGCACAGAC
    CAGTTCGCTT TTCCTGCCTG CTCTCCAGGG CCTTCTCCAC TCTGCAGGGT TAATGAAACA
    CATGCTGAAA GCCAACCGGA AAGTGTGAGT GAAGAAATCG ACATGCATGT GCAAAGTGAT
    CCAGAAAGTC CAACAAATCT AGGCGTGTAC AGGGTTCATG ATACACATGC TGAAAGCCAA
    CTGGAAAGCT TGAGTCCAGA AAGTGACTCT CATGTGCAAA GTGATCCAGG AAGTCCCACA
    AAATCAGGCC TGTACAGGAG GGGAGCAAAA AATAAGATTT CCAGTCGTTC AAACACACTT
    GAGGAGGACT CAGTGAATGT GCAGAGGTTA GACACGGTCT GGAATAAGAG TGATCAAGAT
    TCAACCAATT TGCTCTTTGA TGCTGTGAAG TCTGGCAACT TGGAAAAGTT GGAACAGATG
    CATGAAGAAG GAGCGGATCT CTCGGTCCAG GACCATTTAG GTTGCACCAT CCTCCATCAG
    GCAGTCATTT TAGGTCACAA AGAGATTGTG AAGTTTATCA CTGAAAACGC TCCTGCCTGC
    ATACTGGATG TGACGGAGAA ATCAACAGGA GAGACAGCTC TGCATAAGGC AGCTGCGTTG
    TGCCATAGAA CAATTTGCTG TTATCTGGTG GATGCTGGTG CATCGCTCAT GAAGACAGAT
    CTACAGGGTG AAACTCCAAA GATGTACGCA GAGAGAACTC GAGACTACGA GCTAGCTGAA
    TATTTGGAAA ACCAACAACA CCATCAAATG ATCCAGAGAG ATGACCATGA GACGGCAGTG
    TGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa29153
    Clone ID Related Accession (Same CDS sequence) XM_005174288.3 , XM_005174288.4
    Accession Version XM_005174288.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3900bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product diacylglycerol kinase zeta isoform X2
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005174288.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGACACTT TTTTCCGACG GCATTTTAAG AGAAAGGAAG CAGGGGACGG TGTGCATGAT 
    GACTCCTGTC GACACCGGAG ACCGAGCATT GCAGTGCCAA CCAGCCGTGC CCGACGGCGC
    TCCAGTGTCG GCCTCCCATC CAGTGCATTA ACCCAGCGGC GCCGATCCAG CGTCCAACTT
    CAGGCATTGC CCCGAATCAA CAGCAGTCAA CCGGTACATC AAAAGAGCAG CGGTAGACGG
    AGGTCAAGTA CCACCACCCT GAAAGCCAAT CCTCGCTTTG CTGTGCGCCG TAAGAAAGTA
    GGCAAGCTTC GCAATATAGA CACACACCTC CTAGGCCCAT CCATGCTGCT AGCCAGTCTG
    ATCCAAATGA CTCAAGAAGA GGAAGACGAT GAAGAAGGTC GCAGTCCGCT CCCAGGGCAA
    GTGGAGCTCA AGGGAAGCAG CTCGAAGTTC AGGAGTCACA CAGATGTGCT GCTTTCTGAA
    GGTGACAGTG ATAGTGATGA CTCCAGCGAG CATTCAGAGG AACGTCAATA CTCAAGGATA
    CGCAAAGAGT GTCTGGAGGA GGCTGATGGG TCAGACTGGG GGGTCAAGGA GTCTGGTGTT
    TTATGCCAGC AGGACTGCCT TCCTCTGTCT GTGGTGGCGG AGCGAATCCA GGCCCATCCG
    CTGATTCGAG TCCCGCGCTG CCTCAGGAGG AACTCGTCCC ATTATGGCCA TGCGGATCCA
    GGACCGCATG TGCGCTACTG CCGCAGCAGC CAAAGGAGGC GTCGTAGGAG GGTCTCCACA
    ATCTCCAAAG CAGGAAGCCC ATGGCCTGGA AGAGGCCTGC CGCTGCCTAA CCGAAGAAGC
    TCCTGTTACA GGGGTCCGGC GACTTTCAAT CCTCTCCTGG GGGCTTATTA TGACCCCCGA
    TTGGAGTCCT GGAGTGGATT CTTGTCGAAA GCCATCGCCA AAGCTGGTGT GCAGGATAAC
    GCTGCTCAAT CCGGCACAAT AACACCTGCT AAAAGTGAAC CCAACAAAAA GATATGCAAC
    ACCGTGGATT GGACCGAAAA TGCCCAGTTG GGTCAACACG TGTGGTTTGA GACCAGTCCG
    GCAGGAGATT TCTGTTATGT GGGGGAGTCA AACTGTATCG CAAAGTCCCA GCAAAAGTCT
    CTTCCCCGCC ACAAATGTGC AGCTTGCAAA ATATCAGTTC ACACAGAGTG CATGAATCAG
    CTGGACAAGA TTAACTTCAG GTGTAAGCCA ACTTTTAAAG ATCCAGGGAC GAAAAATGTC
    AGAGAAACTC CAAATATGAA CCATCACTGG GTCCATAGGA GAAGGCAAGA TGGAAAATGC
    CGGCAATGTG GAAAGGGTTT CCAGCAGAAA TTCTCCTTCC ACAGTAAAGA CATTGTGGCC
    ATCAGTTGCT CATGGTGTAA ACAGGCTTAT CACAACAAGG TGAACTGTTT CATGATGCAG
    AGAATCGATG AGCCGTGTTC CCTTGGCGCT CACGCAGCTG TGATCATCCC TCCAACCTGG
    ATTATCAGAA TGCGGAGGCA GCAGGCATCA CTGAAGAGCA GTAAAAGAAG GAAGAGAACG
    TCTCTGAAGG CCAAACCCAG CAAGAAATTA GAGGACGGCA AATGGAGAAA CTTCGTGGTG
    AGAGCGATTC CTTCCCAGCA CATGAAGCCT CTGCTGGTGT TCGTCAATCC GAAGAGTGGT
    GGAAACCAGG GAACGAAAAT CATCCGTACT TTCATGTGGT ACCTGAACCC CCGGCAAGTG
    TTCGACCTGA CTCAGGGAGG TCCGAGAGAG GGGTTAGAAA TGTACAGCAA AGTGCCCAAC
    CTGCGCATCC TTGTTTGTGG TGGCGATGGA ACGGTTGGCT GGATTCTGTC AGTGCTGGAT
    GAGCTTCAGT TGAACCCTCA ACCTGCTGTG GCCGTTCTTC CCCTTGGGAC AGGAAATGAC
    CTGGCCAGGA CCCTCAACTG GGGCGGGGGC TACACAGATG AACCAGTATC CAAGATCCTT
    TCCCATGTTG AAGACGGCAT CATCGTCCAG CTGGATCGCT GGAACCTGAG TGTGGAAGCT
    AATTTAGAGG CCAGCGATGA GGACAAAGAT GAACAACAGA CCGACAAGCT TCCCATTGAC
    GTCTTCAACA ATTACTTCAG CCTCGGGTTT GACGCACATG TGACTTTGGA GTTTCACGAA
    TCCCGAGAGG CTAAACCGGA ACGCTTCAAC AGTCGACTTC GCAATAAGAT GTTTTACGCA
    GGGACAGCAT TCTCAGACTT CCTGATGGGC AGCTCTAAAG ATCTGTCCAA ACACATCAAG
    GTTGTGTGTG ACGGTACTGA CCTCACCAGT AAAATTCAAG ATATGAAAGT GCTGTGCCTG
    CTGTTCCTCA ACATCCCCAG ATATTGCGCT GGTACCATGC CATGGGGGAA CCCAAGTGAG
    AACAATGACT TCGGGCCACA GAAACACGAC GACGGGCTTA TTGAGGTCAT CGGCTTCACC
    ATGACCTCTA TGGCAACTCT TCAGGTTGGA GGACATGGCG AGAGGCTTCA TCAGTGTAAG
    GAGGTGACCC TGACTACTTT TAAGCCCATT CCTGTACAGG TGGATGGGGA ACCCTGTAGA
    CTGGCTCCAT CCGTCATACA CATCACTCGA AGGAACCAAG CCAACATGCT GCAGAAAACC
    AAGAGGAGGA TCTCTCTACC ACAACTGTAT GATCAGCAGC CCATCGCAGA GACAGTGCAG
    ATCCAAGTGA ACCGTATCAG TATGCATGAT TATGAAGCTC TCCATTATGA TAAGGAGCAG
    CTCAAAGAAG CCGCGATTCC CATGGGGATA ATTACAGTTC CTGGGAACAG TGATTTGGAG
    ACGTGTCGGC AGCATATCGA GAGGTTGCAT GAGGAGGGGG ATGGGATGAA TACAGACACA
    CTGCACATGC CAAAGCTTTC ACCAAAATGG TGCTTCCTGG ACTGTACAAC TGCAGATCGT
    TTCTACCGAA TAGATCGTGG TCAGGAGCAT CTAAACTATG TGTCGGAGAT CTCCCAGGAT
    GAGCTTTTCA TCTTGGACCC GGAGCTGGTC ACCAAGGAGA CGGTGGGCAC GTCGCCCGGA
    ATGCCGGGGG CGATGGAAAC GGTTGTTGGG GGATGTGGAT GTTCCAACAG CACAGACCAG
    TTCGCTTTTC CTGCCTGCTC TCCAGGGCCT TCTCCACTCT GCAGGGTTAA TGAAACACAT
    GCTGAAAGCC AACCGGAAAG TGTGAGTGAA GAAATCGACA TGCATGTGCA AAGTGATCCA
    GAAAGTCCAA CAAATCTAGG CGTGTACAGG GTTCATGATA CACATGCTGA AAGCCAACTG
    GAAAGCTTGA GTCCAGAAAG TGACTCTCAT GTGCAAAGTG ATCCAGGAAG TCCCACAAAA
    TCAGGCCTGT ACAGGAGGGG AGCAAAAAAT AAGATTTCCA GTCGTTCAAA CACACTTGAG
    GAGGACTCAG TGAATGTGCA GAGGTTAGAC ACGGTCTGGA ATAAGAGTGA TCAAGATTCA
    ACCAATTTGC TCTTTGATGC TGTGAAGTCT GGCAACTTGG AAAAGTTGGA ACAGATGCAT
    GAAGAAGGAG CGGATCTCTC GGTCCAGGAC CATTTAGGTT GCACCATCCT CCATCAGGCA
    GTCATTTTAG GTCACAAAGA GATTGTGAAG TTTATCACTG AAAACGCTCC TGCCTGCATA
    CTGGATGTGA CGGAGAAATC AACAGGAGAG ACAGCTCTGC ATAAGGCAGC TGCGTTGTGC
    CATAGAACAA TTTGCTGTTA TCTGGTGGAT GCTGGTGCAT CGCTCATGAA GACAGATCTA
    CAGGGTGAAA CTCCAAAGAT GTACGCAGAG AGAACTCGAG ACTACGAGCT AGCTGAATAT
    TTGGAAAACC AACAACACCA TCAAATGATC CAGAGAGATG ACCATGAGAC GGCAGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005174345.1
    CDS300..4199
    Translation

    Target ORF information:

    RefSeq Version XM_005174288.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio diacylglycerol kinase, zeta b (dgkzb), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005174288.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGACACTT TTTTCCGACG GCATTTTAAG AGAAAGGAAG CAGGGGACGG TGTGCATGAT 
    GACTCCTGTC GACACCGGAG ACCGAGCATT GCAGTGCCAA CCAGCCGTGC CCGACGGCGC
    TCCAGTGTCG GCCTCCCATC CAGTGCATTA ACCCAGCGGC GCCGATCCAG CGTCCAACTT
    CAGGCATTGC CCCGAATCAA CAGCAGTCAA CCGGTACATC AAAAGAGCAG CGGTAGACGG
    AGGTCAAGTA CCACCACCCT GAAAGCCAAT CCTCGCTTTG CTGTGCGCCG TAAGAAAGTA
    GGCAAGCTTC GCAATATAGA CACACACCTC CTAGGCCCAT CCATGCTGCT AGCCAGTCTG
    ATCCAAATGA CTCAAGAAGA GGAAGACGAT GAAGAAGGTC GCAGTCCGCT CCCAGGGCAA
    GTGGAGCTCA AGGGAAGCAG CTCGAAGTTC AGGAGTCACA CAGATGTGCT GCTTTCTGAA
    GGTGACAGTG ATAGTGATGA CTCCAGCGAG CATTCAGAGG AACGTCAATA CTCAAGGATA
    CGCAAAGAGT GTCTGGAGGA GGCTGATGGG TCAGACTGGG GGGTCAAGGA GTCTGGTGTT
    TTATGCCAGC AGGACTGCCT TCCTCTGTCT GTGGTGGCGG AGCGAATCCA GGCCCATCCG
    CTGATTCGAG TCCCGCGCTG CCTCAGGAGG AACTCGTCCC ATTATGGCCA TGCGGATCCA
    GGACCGCATG TGCGCTACTG CCGCAGCAGC CAAAGGAGGC GTCGTAGGAG GGTCTCCACA
    ATCTCCAAAG CAGGAAGCCC ATGGCCTGGA AGAGGCCTGC CGCTGCCTAA CCGAAGAAGC
    TCCTGTTACA GGGGTCCGGC GACTTTCAAT CCTCTCCTGG GGGCTTATTA TGACCCCCGA
    TTGGAGTCCT GGAGTGGATT CTTGTCGAAA GCCATCGCCA AAGCTGGTGT GCAGGATAAC
    GCTGCTCAAT CCGGCACAAT AACACCTGCT AAAAGTGAAC CCAACAAAAA GATATGCAAC
    ACCGTGGATT GGACCGAAAA TGCCCAGTTG GGTCAACACG TGTGGTTTGA GACCAGTCCG
    GCAGGAGATT TCTGTTATGT GGGGGAGTCA AACTGTATCG CAAAGTCCCA GCAAAAGTCT
    CTTCCCCGCC ACAAATGTGC AGCTTGCAAA ATATCAGTTC ACACAGAGTG CATGAATCAG
    CTGGACAAGA TTAACTTCAG GTGTAAGCCA ACTTTTAAAG ATCCAGGGAC GAAAAATGTC
    AGAGAAACTC CAAATATGAA CCATCACTGG GTCCATAGGA GAAGGCAAGA TGGAAAATGC
    CGGCAATGTG GAAAGGGTTT CCAGCAGAAA TTCTCCTTCC ACAGTAAAGA CATTGTGGCC
    ATCAGTTGCT CATGGTGTAA ACAGGCTTAT CACAACAAGG TGAACTGTTT CATGATGCAG
    AGAATCGATG AGCCGTGTTC CCTTGGCGCT CACGCAGCTG TGATCATCCC TCCAACCTGG
    ATTATCAGAA TGCGGAGGCA GCAGGCATCA CTGAAGAGCA GTAAAAGAAG GAAGAGAACG
    TCTCTGAAGG CCAAACCCAG CAAGAAATTA GAGGACGGCA AATGGAGAAA CTTCGTGGTG
    AGAGCGATTC CTTCCCAGCA CATGAAGCCT CTGCTGGTGT TCGTCAATCC GAAGAGTGGT
    GGAAACCAGG GAACGAAAAT CATCCGTACT TTCATGTGGT ACCTGAACCC CCGGCAAGTG
    TTCGACCTGA CTCAGGGAGG TCCGAGAGAG GGGTTAGAAA TGTACAGCAA AGTGCCCAAC
    CTGCGCATCC TTGTTTGTGG TGGCGATGGA ACGGTTGGCT GGATTCTGTC AGTGCTGGAT
    GAGCTTCAGT TGAACCCTCA ACCTGCTGTG GCCGTTCTTC CCCTTGGGAC AGGAAATGAC
    CTGGCCAGGA CCCTCAACTG GGGCGGGGGC TACACAGATG AACCAGTATC CAAGATCCTT
    TCCCATGTTG AAGACGGCAT CATCGTCCAG CTGGATCGCT GGAACCTGAG TGTGGAAGCT
    AATTTAGAGG CCAGCGATGA GGACAAAGAT GAACAACAGA CCGACAAGCT TCCCATTGAC
    GTCTTCAACA ATTACTTCAG CCTCGGGTTT GACGCACATG TGACTTTGGA GTTTCACGAA
    TCCCGAGAGG CTAAACCGGA ACGCTTCAAC AGTCGACTTC GCAATAAGAT GTTTTACGCA
    GGGACAGCAT TCTCAGACTT CCTGATGGGC AGCTCTAAAG ATCTGTCCAA ACACATCAAG
    GTTGTGTGTG ACGGTACTGA CCTCACCAGT AAAATTCAAG ATATGAAAGT GCTGTGCCTG
    CTGTTCCTCA ACATCCCCAG ATATTGCGCT GGTACCATGC CATGGGGGAA CCCAAGTGAG
    AACAATGACT TCGGGCCACA GAAACACGAC GACGGGCTTA TTGAGGTCAT CGGCTTCACC
    ATGACCTCTA TGGCAACTCT TCAGGTTGGA GGACATGGCG AGAGGCTTCA TCAGTGTAAG
    GAGGTGACCC TGACTACTTT TAAGCCCATT CCTGTACAGG TGGATGGGGA ACCCTGTAGA
    CTGGCTCCAT CCGTCATACA CATCACTCGA AGGAACCAAG CCAACATGCT GCAGAAAACC
    AAGAGGAGGA TCTCTCTACC ACAACTGTAT GATCAGCAGC CCATCGCAGA GACAGTGCAG
    ATCCAAGTGA ACCGTATCAG TATGCATGAT TATGAAGCTC TCCATTATGA TAAGGAGCAG
    CTCAAAGAAG CCGCGATTCC CATGGGGATA ATTACAGTTC CTGGGAACAG TGATTTGGAG
    ACGTGTCGGC AGCATATCGA GAGGTTGCAT GAGGAGGGGG ATGGGATGAA TACAGACACA
    CTGCACATGC CAAAGCTTTC ACCAAAATGG TGCTTCCTGG ACTGTACAAC TGCAGATCGT
    TTCTACCGAA TAGATCGTGG TCAGGAGCAT CTAAACTATG TGTCGGAGAT CTCCCAGGAT
    GAGCTTTTCA TCTTGGACCC GGAGCTGGTC ACCAAGGAGA CGGTGGGCAC GTCGCCCGGA
    ATGCCGGGGG CGATGGAAAC GGTTGTTGGG GGATGTGGAT GTTCCAACAG CACAGACCAG
    TTCGCTTTTC CTGCCTGCTC TCCAGGGCCT TCTCCACTCT GCAGGGTTAA TGAAACACAT
    GCTGAAAGCC AACCGGAAAG TGTGAGTGAA GAAATCGACA TGCATGTGCA AAGTGATCCA
    GAAAGTCCAA CAAATCTAGG CGTGTACAGG GTTCATGATA CACATGCTGA AAGCCAACTG
    GAAAGCTTGA GTCCAGAAAG TGACTCTCAT GTGCAAAGTG ATCCAGGAAG TCCCACAAAA
    TCAGGCCTGT ACAGGAGGGG AGCAAAAAAT AAGATTTCCA GTCGTTCAAA CACACTTGAG
    GAGGACTCAG TGAATGTGCA GAGGTTAGAC ACGGTCTGGA ATAAGAGTGA TCAAGATTCA
    ACCAATTTGC TCTTTGATGC TGTGAAGTCT GGCAACTTGG AAAAGTTGGA ACAGATGCAT
    GAAGAAGGAG CGGATCTCTC GGTCCAGGAC CATTTAGGTT GCACCATCCT CCATCAGGCA
    GTCATTTTAG GTCACAAAGA GATTGTGAAG TTTATCACTG AAAACGCTCC TGCCTGCATA
    CTGGATGTGA CGGAGAAATC AACAGGAGAG ACAGCTCTGC ATAAGGCAGC TGCGTTGTGC
    CATAGAACAA TTTGCTGTTA TCTGGTGGAT GCTGGTGCAT CGCTCATGAA GACAGATCTA
    CAGGGTGAAA CTCCAAAGAT GTACGCAGAG AGAACTCGAG ACTACGAGCT AGCTGAATAT
    TTGGAAAACC AACAACACCA TCAAATGATC CAGAGAGATG ACCATGAGAC GGCAGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa29153
    Clone ID Related Accession (Same CDS sequence) XM_005174288.3 , XM_005174288.4
    Accession Version XM_005174288.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3900bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product diacylglycerol kinase zeta isoform X2
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005174288.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGACACTT TTTTCCGACG GCATTTTAAG AGAAAGGAAG CAGGGGACGG TGTGCATGAT 
    GACTCCTGTC GACACCGGAG ACCGAGCATT GCAGTGCCAA CCAGCCGTGC CCGACGGCGC
    TCCAGTGTCG GCCTCCCATC CAGTGCATTA ACCCAGCGGC GCCGATCCAG CGTCCAACTT
    CAGGCATTGC CCCGAATCAA CAGCAGTCAA CCGGTACATC AAAAGAGCAG CGGTAGACGG
    AGGTCAAGTA CCACCACCCT GAAAGCCAAT CCTCGCTTTG CTGTGCGCCG TAAGAAAGTA
    GGCAAGCTTC GCAATATAGA CACACACCTC CTAGGCCCAT CCATGCTGCT AGCCAGTCTG
    ATCCAAATGA CTCAAGAAGA GGAAGACGAT GAAGAAGGTC GCAGTCCGCT CCCAGGGCAA
    GTGGAGCTCA AGGGAAGCAG CTCGAAGTTC AGGAGTCACA CAGATGTGCT GCTTTCTGAA
    GGTGACAGTG ATAGTGATGA CTCCAGCGAG CATTCAGAGG AACGTCAATA CTCAAGGATA
    CGCAAAGAGT GTCTGGAGGA GGCTGATGGG TCAGACTGGG GGGTCAAGGA GTCTGGTGTT
    TTATGCCAGC AGGACTGCCT TCCTCTGTCT GTGGTGGCGG AGCGAATCCA GGCCCATCCG
    CTGATTCGAG TCCCGCGCTG CCTCAGGAGG AACTCGTCCC ATTATGGCCA TGCGGATCCA
    GGACCGCATG TGCGCTACTG CCGCAGCAGC CAAAGGAGGC GTCGTAGGAG GGTCTCCACA
    ATCTCCAAAG CAGGAAGCCC ATGGCCTGGA AGAGGCCTGC CGCTGCCTAA CCGAAGAAGC
    TCCTGTTACA GGGGTCCGGC GACTTTCAAT CCTCTCCTGG GGGCTTATTA TGACCCCCGA
    TTGGAGTCCT GGAGTGGATT CTTGTCGAAA GCCATCGCCA AAGCTGGTGT GCAGGATAAC
    GCTGCTCAAT CCGGCACAAT AACACCTGCT AAAAGTGAAC CCAACAAAAA GATATGCAAC
    ACCGTGGATT GGACCGAAAA TGCCCAGTTG GGTCAACACG TGTGGTTTGA GACCAGTCCG
    GCAGGAGATT TCTGTTATGT GGGGGAGTCA AACTGTATCG CAAAGTCCCA GCAAAAGTCT
    CTTCCCCGCC ACAAATGTGC AGCTTGCAAA ATATCAGTTC ACACAGAGTG CATGAATCAG
    CTGGACAAGA TTAACTTCAG GTGTAAGCCA ACTTTTAAAG ATCCAGGGAC GAAAAATGTC
    AGAGAAACTC CAAATATGAA CCATCACTGG GTCCATAGGA GAAGGCAAGA TGGAAAATGC
    CGGCAATGTG GAAAGGGTTT CCAGCAGAAA TTCTCCTTCC ACAGTAAAGA CATTGTGGCC
    ATCAGTTGCT CATGGTGTAA ACAGGCTTAT CACAACAAGG TGAACTGTTT CATGATGCAG
    AGAATCGATG AGCCGTGTTC CCTTGGCGCT CACGCAGCTG TGATCATCCC TCCAACCTGG
    ATTATCAGAA TGCGGAGGCA GCAGGCATCA CTGAAGAGCA GTAAAAGAAG GAAGAGAACG
    TCTCTGAAGG CCAAACCCAG CAAGAAATTA GAGGACGGCA AATGGAGAAA CTTCGTGGTG
    AGAGCGATTC CTTCCCAGCA CATGAAGCCT CTGCTGGTGT TCGTCAATCC GAAGAGTGGT
    GGAAACCAGG GAACGAAAAT CATCCGTACT TTCATGTGGT ACCTGAACCC CCGGCAAGTG
    TTCGACCTGA CTCAGGGAGG TCCGAGAGAG GGGTTAGAAA TGTACAGCAA AGTGCCCAAC
    CTGCGCATCC TTGTTTGTGG TGGCGATGGA ACGGTTGGCT GGATTCTGTC AGTGCTGGAT
    GAGCTTCAGT TGAACCCTCA ACCTGCTGTG GCCGTTCTTC CCCTTGGGAC AGGAAATGAC
    CTGGCCAGGA CCCTCAACTG GGGCGGGGGC TACACAGATG AACCAGTATC CAAGATCCTT
    TCCCATGTTG AAGACGGCAT CATCGTCCAG CTGGATCGCT GGAACCTGAG TGTGGAAGCT
    AATTTAGAGG CCAGCGATGA GGACAAAGAT GAACAACAGA CCGACAAGCT TCCCATTGAC
    GTCTTCAACA ATTACTTCAG CCTCGGGTTT GACGCACATG TGACTTTGGA GTTTCACGAA
    TCCCGAGAGG CTAAACCGGA ACGCTTCAAC AGTCGACTTC GCAATAAGAT GTTTTACGCA
    GGGACAGCAT TCTCAGACTT CCTGATGGGC AGCTCTAAAG ATCTGTCCAA ACACATCAAG
    GTTGTGTGTG ACGGTACTGA CCTCACCAGT AAAATTCAAG ATATGAAAGT GCTGTGCCTG
    CTGTTCCTCA ACATCCCCAG ATATTGCGCT GGTACCATGC CATGGGGGAA CCCAAGTGAG
    AACAATGACT TCGGGCCACA GAAACACGAC GACGGGCTTA TTGAGGTCAT CGGCTTCACC
    ATGACCTCTA TGGCAACTCT TCAGGTTGGA GGACATGGCG AGAGGCTTCA TCAGTGTAAG
    GAGGTGACCC TGACTACTTT TAAGCCCATT CCTGTACAGG TGGATGGGGA ACCCTGTAGA
    CTGGCTCCAT CCGTCATACA CATCACTCGA AGGAACCAAG CCAACATGCT GCAGAAAACC
    AAGAGGAGGA TCTCTCTACC ACAACTGTAT GATCAGCAGC CCATCGCAGA GACAGTGCAG
    ATCCAAGTGA ACCGTATCAG TATGCATGAT TATGAAGCTC TCCATTATGA TAAGGAGCAG
    CTCAAAGAAG CCGCGATTCC CATGGGGATA ATTACAGTTC CTGGGAACAG TGATTTGGAG
    ACGTGTCGGC AGCATATCGA GAGGTTGCAT GAGGAGGGGG ATGGGATGAA TACAGACACA
    CTGCACATGC CAAAGCTTTC ACCAAAATGG TGCTTCCTGG ACTGTACAAC TGCAGATCGT
    TTCTACCGAA TAGATCGTGG TCAGGAGCAT CTAAACTATG TGTCGGAGAT CTCCCAGGAT
    GAGCTTTTCA TCTTGGACCC GGAGCTGGTC ACCAAGGAGA CGGTGGGCAC GTCGCCCGGA
    ATGCCGGGGG CGATGGAAAC GGTTGTTGGG GGATGTGGAT GTTCCAACAG CACAGACCAG
    TTCGCTTTTC CTGCCTGCTC TCCAGGGCCT TCTCCACTCT GCAGGGTTAA TGAAACACAT
    GCTGAAAGCC AACCGGAAAG TGTGAGTGAA GAAATCGACA TGCATGTGCA AAGTGATCCA
    GAAAGTCCAA CAAATCTAGG CGTGTACAGG GTTCATGATA CACATGCTGA AAGCCAACTG
    GAAAGCTTGA GTCCAGAAAG TGACTCTCAT GTGCAAAGTG ATCCAGGAAG TCCCACAAAA
    TCAGGCCTGT ACAGGAGGGG AGCAAAAAAT AAGATTTCCA GTCGTTCAAA CACACTTGAG
    GAGGACTCAG TGAATGTGCA GAGGTTAGAC ACGGTCTGGA ATAAGAGTGA TCAAGATTCA
    ACCAATTTGC TCTTTGATGC TGTGAAGTCT GGCAACTTGG AAAAGTTGGA ACAGATGCAT
    GAAGAAGGAG CGGATCTCTC GGTCCAGGAC CATTTAGGTT GCACCATCCT CCATCAGGCA
    GTCATTTTAG GTCACAAAGA GATTGTGAAG TTTATCACTG AAAACGCTCC TGCCTGCATA
    CTGGATGTGA CGGAGAAATC AACAGGAGAG ACAGCTCTGC ATAAGGCAGC TGCGTTGTGC
    CATAGAACAA TTTGCTGTTA TCTGGTGGAT GCTGGTGCAT CGCTCATGAA GACAGATCTA
    CAGGGTGAAA CTCCAAAGAT GTACGCAGAG AGAACTCGAG ACTACGAGCT AGCTGAATAT
    TTGGAAAACC AACAACACCA TCAAATGATC CAGAGAGATG ACCATGAGAC GGCAGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005174345.1
    CDS308..4207
    Translation

    Target ORF information:

    RefSeq Version XM_005174288.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio diacylglycerol kinase, zeta b (dgkzb), transcript variant X2, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005174288.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGACACTT TTTTCCGACG GCATTTTAAG AGAAAGGAAG CAGGGGACGG TGTGCATGAT 
    GACTCCTGTC GACACCGGAG ACCGAGCATT GCAGTGCCAA CCAGCCGTGC CCGACGGCGC
    TCCAGTGTCG GCCTCCCATC CAGTGCATTA ACCCAGCGGC GCCGATCCAG CGTCCAACTT
    CAGGCATTGC CCCGAATCAA CAGCAGTCAA CCGGTACATC AAAAGAGCAG CGGTAGACGG
    AGGTCAAGTA CCACCACCCT GAAAGCCAAT CCTCGCTTTG CTGTGCGCCG TAAGAAAGTA
    GGCAAGCTTC GCAATATAGA CACACACCTC CTAGGCCCAT CCATGCTGCT AGCCAGTCTG
    ATCCAAATGA CTCAAGAAGA GGAAGACGAT GAAGAAGGTC GCAGTCCGCT CCCAGGGCAA
    GTGGAGCTCA AGGGAAGCAG CTCGAAGTTC AGGAGTCACA CAGATGTGCT GCTTTCTGAA
    GGTGACAGTG ATAGTGATGA CTCCAGCGAG CATTCAGAGG AACGTCAATA CTCAAGGATA
    CGCAAAGAGT GTCTGGAGGA GGCTGATGGG TCAGACTGGG GGGTCAAGGA GTCTGGTGTT
    TTATGCCAGC AGGACTGCCT TCCTCTGTCT GTGGTGGCGG AGCGAATCCA GGCCCATCCG
    CTGATTCGAG TCCCGCGCTG CCTCAGGAGG AACTCGTCCC ATTATGGCCA TGCGGATCCA
    GGACCGCATG TGCGCTACTG CCGCAGCAGC CAAAGGAGGC GTCGTAGGAG GGTCTCCACA
    ATCTCCAAAG CAGGAAGCCC ATGGCCTGGA AGAGGCCTGC CGCTGCCTAA CCGAAGAAGC
    TCCTGTTACA GGGGTCCGGC GACTTTCAAT CCTCTCCTGG GGGCTTATTA TGACCCCCGA
    TTGGAGTCCT GGAGTGGATT CTTGTCGAAA GCCATCGCCA AAGCTGGTGT GCAGGATAAC
    GCTGCTCAAT CCGGCACAAT AACACCTGCT AAAAGTGAAC CCAACAAAAA GATATGCAAC
    ACCGTGGATT GGACCGAAAA TGCCCAGTTG GGTCAACACG TGTGGTTTGA GACCAGTCCG
    GCAGGAGATT TCTGTTATGT GGGGGAGTCA AACTGTATCG CAAAGTCCCA GCAAAAGTCT
    CTTCCCCGCC ACAAATGTGC AGCTTGCAAA ATATCAGTTC ACACAGAGTG CATGAATCAG
    CTGGACAAGA TTAACTTCAG GTGTAAGCCA ACTTTTAAAG ATCCAGGGAC GAAAAATGTC
    AGAGAAACTC CAAATATGAA CCATCACTGG GTCCATAGGA GAAGGCAAGA TGGAAAATGC
    CGGCAATGTG GAAAGGGTTT CCAGCAGAAA TTCTCCTTCC ACAGTAAAGA CATTGTGGCC
    ATCAGTTGCT CATGGTGTAA ACAGGCTTAT CACAACAAGG TGAACTGTTT CATGATGCAG
    AGAATCGATG AGCCGTGTTC CCTTGGCGCT CACGCAGCTG TGATCATCCC TCCAACCTGG
    ATTATCAGAA TGCGGAGGCA GCAGGCATCA CTGAAGAGCA GTAAAAGAAG GAAGAGAACG
    TCTCTGAAGG CCAAACCCAG CAAGAAATTA GAGGACGGCA AATGGAGAAA CTTCGTGGTG
    AGAGCGATTC CTTCCCAGCA CATGAAGCCT CTGCTGGTGT TCGTCAATCC GAAGAGTGGT
    GGAAACCAGG GAACGAAAAT CATCCGTACT TTCATGTGGT ACCTGAACCC CCGGCAAGTG
    TTCGACCTGA CTCAGGGAGG TCCGAGAGAG GGGTTAGAAA TGTACAGCAA AGTGCCCAAC
    CTGCGCATCC TTGTTTGTGG TGGCGATGGA ACGGTTGGCT GGATTCTGTC AGTGCTGGAT
    GAGCTTCAGT TGAACCCTCA ACCTGCTGTG GCCGTTCTTC CCCTTGGGAC AGGAAATGAC
    CTGGCCAGGA CCCTCAACTG GGGCGGGGGC TACACAGATG AACCAGTATC CAAGATCCTT
    TCCCATGTTG AAGACGGCAT CATCGTCCAG CTGGATCGCT GGAACCTGAG TGTGGAAGCT
    AATTTAGAGG CCAGCGATGA GGACAAAGAT GAACAACAGA CCGACAAGCT TCCCATTGAC
    GTCTTCAACA ATTACTTCAG CCTCGGGTTT GACGCACATG TGACTTTGGA GTTTCACGAA
    TCCCGAGAGG CTAAACCGGA ACGCTTCAAC AGTCGACTTC GCAATAAGAT GTTTTACGCA
    GGGACAGCAT TCTCAGACTT CCTGATGGGC AGCTCTAAAG ATCTGTCCAA ACACATCAAG
    GTTGTGTGTG ACGGTACTGA CCTCACCAGT AAAATTCAAG ATATGAAAGT GCTGTGCCTG
    CTGTTCCTCA ACATCCCCAG ATATTGCGCT GGTACCATGC CATGGGGGAA CCCAAGTGAG
    AACAATGACT TCGGGCCACA GAAACACGAC GACGGGCTTA TTGAGGTCAT CGGCTTCACC
    ATGACCTCTA TGGCAACTCT TCAGGTTGGA GGACATGGCG AGAGGCTTCA TCAGTGTAAG
    GAGGTGACCC TGACTACTTT TAAGCCCATT CCTGTACAGG TGGATGGGGA ACCCTGTAGA
    CTGGCTCCAT CCGTCATACA CATCACTCGA AGGAACCAAG CCAACATGCT GCAGAAAACC
    AAGAGGAGGA TCTCTCTACC ACAACTGTAT GATCAGCAGC CCATCGCAGA GACAGTGCAG
    ATCCAAGTGA ACCGTATCAG TATGCATGAT TATGAAGCTC TCCATTATGA TAAGGAGCAG
    CTCAAAGAAG CCGCGATTCC CATGGGGATA ATTACAGTTC CTGGGAACAG TGATTTGGAG
    ACGTGTCGGC AGCATATCGA GAGGTTGCAT GAGGAGGGGG ATGGGATGAA TACAGACACA
    CTGCACATGC CAAAGCTTTC ACCAAAATGG TGCTTCCTGG ACTGTACAAC TGCAGATCGT
    TTCTACCGAA TAGATCGTGG TCAGGAGCAT CTAAACTATG TGTCGGAGAT CTCCCAGGAT
    GAGCTTTTCA TCTTGGACCC GGAGCTGGTC ACCAAGGAGA CGGTGGGCAC GTCGCCCGGA
    ATGCCGGGGG CGATGGAAAC GGTTGTTGGG GGATGTGGAT GTTCCAACAG CACAGACCAG
    TTCGCTTTTC CTGCCTGCTC TCCAGGGCCT TCTCCACTCT GCAGGGTTAA TGAAACACAT
    GCTGAAAGCC AACCGGAAAG TGTGAGTGAA GAAATCGACA TGCATGTGCA AAGTGATCCA
    GAAAGTCCAA CAAATCTAGG CGTGTACAGG GTTCATGATA CACATGCTGA AAGCCAACTG
    GAAAGCTTGA GTCCAGAAAG TGACTCTCAT GTGCAAAGTG ATCCAGGAAG TCCCACAAAA
    TCAGGCCTGT ACAGGAGGGG AGCAAAAAAT AAGATTTCCA GTCGTTCAAA CACACTTGAG
    GAGGACTCAG TGAATGTGCA GAGGTTAGAC ACGGTCTGGA ATAAGAGTGA TCAAGATTCA
    ACCAATTTGC TCTTTGATGC TGTGAAGTCT GGCAACTTGG AAAAGTTGGA ACAGATGCAT
    GAAGAAGGAG CGGATCTCTC GGTCCAGGAC CATTTAGGTT GCACCATCCT CCATCAGGCA
    GTCATTTTAG GTCACAAAGA GATTGTGAAG TTTATCACTG AAAACGCTCC TGCCTGCATA
    CTGGATGTGA CGGAGAAATC AACAGGAGAG ACAGCTCTGC ATAAGGCAGC TGCGTTGTGC
    CATAGAACAA TTTGCTGTTA TCTGGTGGAT GCTGGTGCAT CGCTCATGAA GACAGATCTA
    CAGGGTGAAA CTCCAAAGAT GTACGCAGAG AGAACTCGAG ACTACGAGCT AGCTGAATAT
    TTGGAAAACC AACAACACCA TCAAATGATC CAGAGAGATG ACCATGAGAC GGCAGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa29154
    Clone ID Related Accession (Same CDS sequence) XM_005174289.4 , XM_005174289.3
    Accession Version XM_005174289.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3897bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product diacylglycerol kinase zeta isoform X3
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005174289.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGACACTT TTTTCCGACG GCATTTTAAG AGAAAGGAAG CAGGGGACGG TGTGCATGAT 
    GACTCCTGTC GACACCGGAG ACCGAGCATT GCAGTGCCAA CCAGCCGTGC CCGACGGCGC
    TCCAGTGTCG GCCTCCCATC CAGTGCATTA ACCCAGCGGC GCCGATCCAG CGTCCAACTT
    CAGGCATTGC CCCGAATCAA CAGCAGTCAA CCGGTACATC AAAAGAGCAG CGGTAGACGG
    AGGTCAAGTA CCACCACCCT GAAAGCCAAT CCTCGCTTTG CTGTGCGCCG TAAGAAAGTA
    GGCAAGCTTC GCAATATAGA CACACACCTC CTAGGCCCAT CCATGCTGCT AGCCAGTCTG
    ATCCAAATGA CTCAAGAAGA GGAAGACGAT GAAGAAGGTC GCAGTCCGCT CCCAGGGCAA
    GTGGAGCTCA AGGGAAGCAG CTCGAAGTTC AGGAGTCACA CAGATGTGCT GCTTTCTGAA
    GGTGACAGTG ATAGTGATGA CTCCAGCGAG CATTCAGAGG AACGTCAATA CTCAAGGATA
    CGCAAAGAGT GTCTGGAGGA GGCTGATGGG TCAGACTGGG GGGTCAAGGA GTCTGGTGTT
    TTATGCCAGC AGGACTGCCT TCCTCTGTCT GTGGTGGCGG AGCGAATCCA GGCCCATCCG
    CTGATTCGAG TCCCGCGCTG CCTCAGGAGG AACTCGTCCC ATTATGGCCA TGCGGATCCA
    GGACCGCATG TGCGCTACTG CCGCAGCAGC CAAAGGAGGC GTCGTAGGAG GGTCTCCACA
    ATCTCCAAAG CAGGAAGCCC ATGGCCTGGA AGAGGCCTGC CGCTGCCTAA CCGAAGAAGC
    TCCTGTTACA GGGGTCCGGC GACTTTCAAT CCTCTCCTGG GGGCTTATTA TGACCCCCGA
    TTGGAGTCCT GGAGTGGATT CTTGTCGAAA GCCATCGCCA AAGCTGGTGT GCAGGATAAC
    GCTGCTCAAT CCGGCACAAT AACACCTGCT AAAAGTGAAC CCAACAAAAA GATATGCAAC
    ACCGTGGATT GGACCGAAAA TGCCCAGTTG GGTCAACACG TGTGGTTTGA GACCAGTCCG
    GCAGGAGATT TCTGTTATGT GGGGGAGTCA AACTGTATCG CAAAGTCCCA GCAAAAGTCT
    CTTCCCCGCC ACAAATGTGC AGCTTGCAAA ATATCAGTTC ACACAGAGTG CATGAATCAG
    CTGGACAAGA TTAACTTCAG GTGTAAGCCA ACTTTTAAAG ATCCAGGGAC GAAAAATGTC
    AGAGAAACTC CAAATATGAA CCATCACTGG GTCCATAGGA GAAGGCAAGA TGGAAAATGC
    CGGCAATGTG GAAAGGGTTT CCAGCAGAAA TTCTCCTTCC ACAGTAAAGA CATTGTGGCC
    ATCAGTTGCT CATGGTGTAA ACAGGCTTAT CACAACAAGG TGAACTGTTT CATGATGCAG
    AGAATCGATG AGCCGTGTTC CCTTGGCGCT CACGCAGCTG TGATCATCCC TCCAACCTGG
    ATTATCAGAA TGCGGAGGCA GCAGGCATCA CTGAAGAGCA GTAAAAGAAG GAAGAGAACG
    TCTCTGAAGG CCAAACCCAG CAAGAAATTA GAGCAGGACG GCAAATGGAG AAACTTCGTG
    GTGAGAGCGA TTCCTTCCCA GCACATGAAG CCTCTGCTGG TGTTCGTCAA TCCGAAGAGT
    GGTGGAAACC AGGGAACGAA AATCATCCGT ACTTTCATGT GGTACCTGAA CCCCCGGCAA
    GTGTTCGACC TGACTCAGGG AGGTCCGAGA GAGGGGTTAG AAATGTACAG CAAAGTGCCC
    AACCTGCGCA TCCTTGTTTG TGGTGGCGAT GGAACGGTTG GCTGGATTCT GTCAGTGCTG
    GATGAGCTTC AGTTGAACCC TCAACCTGCT GTGGCCGTTC TTCCCCTTGG GACAGGAAAT
    GACCTGGCCA GGACCCTCAA CTGGGGCGGG GGCTACACAG ATGAACCAGT ATCCAAGATC
    CTTTCCCATG TTGAAGACGG CATCATCGTC CAGCTGGATC GCTGGAACCT GAGTGTGGAA
    GCTAATTTAG AGGCCAGCGA TGAGGACAAA GATGAACAAC AGACCGACAA GCTTCCCATT
    GACGTCTTCA ACAATTACTT CAGCCTCGGG TTTGACGCAC ATGTGACTTT GGAGTTTCAC
    GAATCCCGAG AGGCTAAACC GGAACGCTTC AACAGTCGAC TTCGCAATAA GATGTTTTAC
    GCAGGGACAG CATTCTCAGA CTTCCTGATG GGCAGCTCTA AAGATCTGTC CAAACACATC
    AAGGTTGTGT GTGACGGTAC TGACCTCACC AGTAAAATTC AAGATATGAA AGTGCTGTGC
    CTGCTGTTCC TCAACATCCC CAGATATTGC GCTGGTACCA TGCCATGGGG GAACCCAAGT
    GAGAACAATG ACTTCGGGCC ACAGAAACAC GACGACGGGC TTATTGAGGT CATCGGCTTC
    ACCATGACCT CTATGGCAAC TCTTCAGGTT GGAGGACATG GCGAGAGGCT TCATCAGTGT
    AAGGAGGTGA CCCTGACTAC TTTTAAGCCC ATTCCTGTAC AGGTGGATGG GGAACCCTGT
    AGACTGGCTC CATCCGTCAT ACACATCACT CGAAGGAACC AAGCCAACAT GCTGCAGAAA
    ACCAAGAGGA GGATCTCTCT ACCACAACTT CAGCAGCCCA TCGCAGAGAC AGTGCAGATC
    CAAGTGAACC GTATCAGTAT GCATGATTAT GAAGCTCTCC ATTATGATAA GGAGCAGCTC
    AAAGAAGCCG CGATTCCCAT GGGGATAATT ACAGTTCCTG GGAACAGTGA TTTGGAGACG
    TGTCGGCAGC ATATCGAGAG GTTGCATGAG GAGGGGGATG GGATGAATAC AGACACACTG
    CACATGCCAA AGCTTTCACC AAAATGGTGC TTCCTGGACT GTACAACTGC AGATCGTTTC
    TACCGAATAG ATCGTGGTCA GGAGCATCTA AACTATGTGT CGGAGATCTC CCAGGATGAG
    CTTTTCATCT TGGACCCGGA GCTGGTCACC AAGGAGACGG TGGGCACGTC GCCCGGAATG
    CCGGGGGCGA TGGAAACGGT TGTTGGGGGA TGTGGATGTT CCAACAGCAC AGACCAGTTC
    GCTTTTCCTG CCTGCTCTCC AGGGCCTTCT CCACTCTGCA GGGTTAATGA AACACATGCT
    GAAAGCCAAC CGGAAAGTGT GAGTGAAGAA ATCGACATGC ATGTGCAAAG TGATCCAGAA
    AGTCCAACAA ATCTAGGCGT GTACAGGGTT CATGATACAC ATGCTGAAAG CCAACTGGAA
    AGCTTGAGTC CAGAAAGTGA CTCTCATGTG CAAAGTGATC CAGGAAGTCC CACAAAATCA
    GGCCTGTACA GGAGGGGAGC AAAAAATAAG ATTTCCAGTC GTTCAAACAC ACTTGAGGAG
    GACTCAGTGA ATGTGCAGAG GTTAGACACG GTCTGGAATA AGAGTGATCA AGATTCAACC
    AATTTGCTCT TTGATGCTGT GAAGTCTGGC AACTTGGAAA AGTTGGAACA GATGCATGAA
    GAAGGAGCGG ATCTCTCGGT CCAGGACCAT TTAGGTTGCA CCATCCTCCA TCAGGCAGTC
    ATTTTAGGTC ACAAAGAGAT TGTGAAGTTT ATCACTGAAA ACGCTCCTGC CTGCATACTG
    GATGTGACGG AGAAATCAAC AGGAGAGACA GCTCTGCATA AGGCAGCTGC GTTGTGCCAT
    AGAACAATTT GCTGTTATCT GGTGGATGCT GGTGCATCGC TCATGAAGAC AGATCTACAG
    GGTGAAACTC CAAAGATGTA CGCAGAGAGA ACTCGAGACT ACGAGCTAGC TGAATATTTG
    GAAAACCAAC AACACCATCA AATGATCCAG AGAGATGACC ATGAGACGGC AGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005174346.1
    CDS308..4204
    Translation

    Target ORF information:

    RefSeq Version XM_005174289.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio diacylglycerol kinase, zeta b (dgkzb), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005174289.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGACACTT TTTTCCGACG GCATTTTAAG AGAAAGGAAG CAGGGGACGG TGTGCATGAT 
    GACTCCTGTC GACACCGGAG ACCGAGCATT GCAGTGCCAA CCAGCCGTGC CCGACGGCGC
    TCCAGTGTCG GCCTCCCATC CAGTGCATTA ACCCAGCGGC GCCGATCCAG CGTCCAACTT
    CAGGCATTGC CCCGAATCAA CAGCAGTCAA CCGGTACATC AAAAGAGCAG CGGTAGACGG
    AGGTCAAGTA CCACCACCCT GAAAGCCAAT CCTCGCTTTG CTGTGCGCCG TAAGAAAGTA
    GGCAAGCTTC GCAATATAGA CACACACCTC CTAGGCCCAT CCATGCTGCT AGCCAGTCTG
    ATCCAAATGA CTCAAGAAGA GGAAGACGAT GAAGAAGGTC GCAGTCCGCT CCCAGGGCAA
    GTGGAGCTCA AGGGAAGCAG CTCGAAGTTC AGGAGTCACA CAGATGTGCT GCTTTCTGAA
    GGTGACAGTG ATAGTGATGA CTCCAGCGAG CATTCAGAGG AACGTCAATA CTCAAGGATA
    CGCAAAGAGT GTCTGGAGGA GGCTGATGGG TCAGACTGGG GGGTCAAGGA GTCTGGTGTT
    TTATGCCAGC AGGACTGCCT TCCTCTGTCT GTGGTGGCGG AGCGAATCCA GGCCCATCCG
    CTGATTCGAG TCCCGCGCTG CCTCAGGAGG AACTCGTCCC ATTATGGCCA TGCGGATCCA
    GGACCGCATG TGCGCTACTG CCGCAGCAGC CAAAGGAGGC GTCGTAGGAG GGTCTCCACA
    ATCTCCAAAG CAGGAAGCCC ATGGCCTGGA AGAGGCCTGC CGCTGCCTAA CCGAAGAAGC
    TCCTGTTACA GGGGTCCGGC GACTTTCAAT CCTCTCCTGG GGGCTTATTA TGACCCCCGA
    TTGGAGTCCT GGAGTGGATT CTTGTCGAAA GCCATCGCCA AAGCTGGTGT GCAGGATAAC
    GCTGCTCAAT CCGGCACAAT AACACCTGCT AAAAGTGAAC CCAACAAAAA GATATGCAAC
    ACCGTGGATT GGACCGAAAA TGCCCAGTTG GGTCAACACG TGTGGTTTGA GACCAGTCCG
    GCAGGAGATT TCTGTTATGT GGGGGAGTCA AACTGTATCG CAAAGTCCCA GCAAAAGTCT
    CTTCCCCGCC ACAAATGTGC AGCTTGCAAA ATATCAGTTC ACACAGAGTG CATGAATCAG
    CTGGACAAGA TTAACTTCAG GTGTAAGCCA ACTTTTAAAG ATCCAGGGAC GAAAAATGTC
    AGAGAAACTC CAAATATGAA CCATCACTGG GTCCATAGGA GAAGGCAAGA TGGAAAATGC
    CGGCAATGTG GAAAGGGTTT CCAGCAGAAA TTCTCCTTCC ACAGTAAAGA CATTGTGGCC
    ATCAGTTGCT CATGGTGTAA ACAGGCTTAT CACAACAAGG TGAACTGTTT CATGATGCAG
    AGAATCGATG AGCCGTGTTC CCTTGGCGCT CACGCAGCTG TGATCATCCC TCCAACCTGG
    ATTATCAGAA TGCGGAGGCA GCAGGCATCA CTGAAGAGCA GTAAAAGAAG GAAGAGAACG
    TCTCTGAAGG CCAAACCCAG CAAGAAATTA GAGCAGGACG GCAAATGGAG AAACTTCGTG
    GTGAGAGCGA TTCCTTCCCA GCACATGAAG CCTCTGCTGG TGTTCGTCAA TCCGAAGAGT
    GGTGGAAACC AGGGAACGAA AATCATCCGT ACTTTCATGT GGTACCTGAA CCCCCGGCAA
    GTGTTCGACC TGACTCAGGG AGGTCCGAGA GAGGGGTTAG AAATGTACAG CAAAGTGCCC
    AACCTGCGCA TCCTTGTTTG TGGTGGCGAT GGAACGGTTG GCTGGATTCT GTCAGTGCTG
    GATGAGCTTC AGTTGAACCC TCAACCTGCT GTGGCCGTTC TTCCCCTTGG GACAGGAAAT
    GACCTGGCCA GGACCCTCAA CTGGGGCGGG GGCTACACAG ATGAACCAGT ATCCAAGATC
    CTTTCCCATG TTGAAGACGG CATCATCGTC CAGCTGGATC GCTGGAACCT GAGTGTGGAA
    GCTAATTTAG AGGCCAGCGA TGAGGACAAA GATGAACAAC AGACCGACAA GCTTCCCATT
    GACGTCTTCA ACAATTACTT CAGCCTCGGG TTTGACGCAC ATGTGACTTT GGAGTTTCAC
    GAATCCCGAG AGGCTAAACC GGAACGCTTC AACAGTCGAC TTCGCAATAA GATGTTTTAC
    GCAGGGACAG CATTCTCAGA CTTCCTGATG GGCAGCTCTA AAGATCTGTC CAAACACATC
    AAGGTTGTGT GTGACGGTAC TGACCTCACC AGTAAAATTC AAGATATGAA AGTGCTGTGC
    CTGCTGTTCC TCAACATCCC CAGATATTGC GCTGGTACCA TGCCATGGGG GAACCCAAGT
    GAGAACAATG ACTTCGGGCC ACAGAAACAC GACGACGGGC TTATTGAGGT CATCGGCTTC
    ACCATGACCT CTATGGCAAC TCTTCAGGTT GGAGGACATG GCGAGAGGCT TCATCAGTGT
    AAGGAGGTGA CCCTGACTAC TTTTAAGCCC ATTCCTGTAC AGGTGGATGG GGAACCCTGT
    AGACTGGCTC CATCCGTCAT ACACATCACT CGAAGGAACC AAGCCAACAT GCTGCAGAAA
    ACCAAGAGGA GGATCTCTCT ACCACAACTT CAGCAGCCCA TCGCAGAGAC AGTGCAGATC
    CAAGTGAACC GTATCAGTAT GCATGATTAT GAAGCTCTCC ATTATGATAA GGAGCAGCTC
    AAAGAAGCCG CGATTCCCAT GGGGATAATT ACAGTTCCTG GGAACAGTGA TTTGGAGACG
    TGTCGGCAGC ATATCGAGAG GTTGCATGAG GAGGGGGATG GGATGAATAC AGACACACTG
    CACATGCCAA AGCTTTCACC AAAATGGTGC TTCCTGGACT GTACAACTGC AGATCGTTTC
    TACCGAATAG ATCGTGGTCA GGAGCATCTA AACTATGTGT CGGAGATCTC CCAGGATGAG
    CTTTTCATCT TGGACCCGGA GCTGGTCACC AAGGAGACGG TGGGCACGTC GCCCGGAATG
    CCGGGGGCGA TGGAAACGGT TGTTGGGGGA TGTGGATGTT CCAACAGCAC AGACCAGTTC
    GCTTTTCCTG CCTGCTCTCC AGGGCCTTCT CCACTCTGCA GGGTTAATGA AACACATGCT
    GAAAGCCAAC CGGAAAGTGT GAGTGAAGAA ATCGACATGC ATGTGCAAAG TGATCCAGAA
    AGTCCAACAA ATCTAGGCGT GTACAGGGTT CATGATACAC ATGCTGAAAG CCAACTGGAA
    AGCTTGAGTC CAGAAAGTGA CTCTCATGTG CAAAGTGATC CAGGAAGTCC CACAAAATCA
    GGCCTGTACA GGAGGGGAGC AAAAAATAAG ATTTCCAGTC GTTCAAACAC ACTTGAGGAG
    GACTCAGTGA ATGTGCAGAG GTTAGACACG GTCTGGAATA AGAGTGATCA AGATTCAACC
    AATTTGCTCT TTGATGCTGT GAAGTCTGGC AACTTGGAAA AGTTGGAACA GATGCATGAA
    GAAGGAGCGG ATCTCTCGGT CCAGGACCAT TTAGGTTGCA CCATCCTCCA TCAGGCAGTC
    ATTTTAGGTC ACAAAGAGAT TGTGAAGTTT ATCACTGAAA ACGCTCCTGC CTGCATACTG
    GATGTGACGG AGAAATCAAC AGGAGAGACA GCTCTGCATA AGGCAGCTGC GTTGTGCCAT
    AGAACAATTT GCTGTTATCT GGTGGATGCT GGTGCATCGC TCATGAAGAC AGATCTACAG
    GGTGAAACTC CAAAGATGTA CGCAGAGAGA ACTCGAGACT ACGAGCTAGC TGAATATTTG
    GAAAACCAAC AACACCATCA AATGATCCAG AGAGATGACC ATGAGACGGC AGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa29154
    Clone ID Related Accession (Same CDS sequence) XM_005174289.4 , XM_005174289.3
    Accession Version XM_005174289.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3897bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product diacylglycerol kinase zeta isoform X3
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005174289.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGACACTT TTTTCCGACG GCATTTTAAG AGAAAGGAAG CAGGGGACGG TGTGCATGAT 
    GACTCCTGTC GACACCGGAG ACCGAGCATT GCAGTGCCAA CCAGCCGTGC CCGACGGCGC
    TCCAGTGTCG GCCTCCCATC CAGTGCATTA ACCCAGCGGC GCCGATCCAG CGTCCAACTT
    CAGGCATTGC CCCGAATCAA CAGCAGTCAA CCGGTACATC AAAAGAGCAG CGGTAGACGG
    AGGTCAAGTA CCACCACCCT GAAAGCCAAT CCTCGCTTTG CTGTGCGCCG TAAGAAAGTA
    GGCAAGCTTC GCAATATAGA CACACACCTC CTAGGCCCAT CCATGCTGCT AGCCAGTCTG
    ATCCAAATGA CTCAAGAAGA GGAAGACGAT GAAGAAGGTC GCAGTCCGCT CCCAGGGCAA
    GTGGAGCTCA AGGGAAGCAG CTCGAAGTTC AGGAGTCACA CAGATGTGCT GCTTTCTGAA
    GGTGACAGTG ATAGTGATGA CTCCAGCGAG CATTCAGAGG AACGTCAATA CTCAAGGATA
    CGCAAAGAGT GTCTGGAGGA GGCTGATGGG TCAGACTGGG GGGTCAAGGA GTCTGGTGTT
    TTATGCCAGC AGGACTGCCT TCCTCTGTCT GTGGTGGCGG AGCGAATCCA GGCCCATCCG
    CTGATTCGAG TCCCGCGCTG CCTCAGGAGG AACTCGTCCC ATTATGGCCA TGCGGATCCA
    GGACCGCATG TGCGCTACTG CCGCAGCAGC CAAAGGAGGC GTCGTAGGAG GGTCTCCACA
    ATCTCCAAAG CAGGAAGCCC ATGGCCTGGA AGAGGCCTGC CGCTGCCTAA CCGAAGAAGC
    TCCTGTTACA GGGGTCCGGC GACTTTCAAT CCTCTCCTGG GGGCTTATTA TGACCCCCGA
    TTGGAGTCCT GGAGTGGATT CTTGTCGAAA GCCATCGCCA AAGCTGGTGT GCAGGATAAC
    GCTGCTCAAT CCGGCACAAT AACACCTGCT AAAAGTGAAC CCAACAAAAA GATATGCAAC
    ACCGTGGATT GGACCGAAAA TGCCCAGTTG GGTCAACACG TGTGGTTTGA GACCAGTCCG
    GCAGGAGATT TCTGTTATGT GGGGGAGTCA AACTGTATCG CAAAGTCCCA GCAAAAGTCT
    CTTCCCCGCC ACAAATGTGC AGCTTGCAAA ATATCAGTTC ACACAGAGTG CATGAATCAG
    CTGGACAAGA TTAACTTCAG GTGTAAGCCA ACTTTTAAAG ATCCAGGGAC GAAAAATGTC
    AGAGAAACTC CAAATATGAA CCATCACTGG GTCCATAGGA GAAGGCAAGA TGGAAAATGC
    CGGCAATGTG GAAAGGGTTT CCAGCAGAAA TTCTCCTTCC ACAGTAAAGA CATTGTGGCC
    ATCAGTTGCT CATGGTGTAA ACAGGCTTAT CACAACAAGG TGAACTGTTT CATGATGCAG
    AGAATCGATG AGCCGTGTTC CCTTGGCGCT CACGCAGCTG TGATCATCCC TCCAACCTGG
    ATTATCAGAA TGCGGAGGCA GCAGGCATCA CTGAAGAGCA GTAAAAGAAG GAAGAGAACG
    TCTCTGAAGG CCAAACCCAG CAAGAAATTA GAGCAGGACG GCAAATGGAG AAACTTCGTG
    GTGAGAGCGA TTCCTTCCCA GCACATGAAG CCTCTGCTGG TGTTCGTCAA TCCGAAGAGT
    GGTGGAAACC AGGGAACGAA AATCATCCGT ACTTTCATGT GGTACCTGAA CCCCCGGCAA
    GTGTTCGACC TGACTCAGGG AGGTCCGAGA GAGGGGTTAG AAATGTACAG CAAAGTGCCC
    AACCTGCGCA TCCTTGTTTG TGGTGGCGAT GGAACGGTTG GCTGGATTCT GTCAGTGCTG
    GATGAGCTTC AGTTGAACCC TCAACCTGCT GTGGCCGTTC TTCCCCTTGG GACAGGAAAT
    GACCTGGCCA GGACCCTCAA CTGGGGCGGG GGCTACACAG ATGAACCAGT ATCCAAGATC
    CTTTCCCATG TTGAAGACGG CATCATCGTC CAGCTGGATC GCTGGAACCT GAGTGTGGAA
    GCTAATTTAG AGGCCAGCGA TGAGGACAAA GATGAACAAC AGACCGACAA GCTTCCCATT
    GACGTCTTCA ACAATTACTT CAGCCTCGGG TTTGACGCAC ATGTGACTTT GGAGTTTCAC
    GAATCCCGAG AGGCTAAACC GGAACGCTTC AACAGTCGAC TTCGCAATAA GATGTTTTAC
    GCAGGGACAG CATTCTCAGA CTTCCTGATG GGCAGCTCTA AAGATCTGTC CAAACACATC
    AAGGTTGTGT GTGACGGTAC TGACCTCACC AGTAAAATTC AAGATATGAA AGTGCTGTGC
    CTGCTGTTCC TCAACATCCC CAGATATTGC GCTGGTACCA TGCCATGGGG GAACCCAAGT
    GAGAACAATG ACTTCGGGCC ACAGAAACAC GACGACGGGC TTATTGAGGT CATCGGCTTC
    ACCATGACCT CTATGGCAAC TCTTCAGGTT GGAGGACATG GCGAGAGGCT TCATCAGTGT
    AAGGAGGTGA CCCTGACTAC TTTTAAGCCC ATTCCTGTAC AGGTGGATGG GGAACCCTGT
    AGACTGGCTC CATCCGTCAT ACACATCACT CGAAGGAACC AAGCCAACAT GCTGCAGAAA
    ACCAAGAGGA GGATCTCTCT ACCACAACTT CAGCAGCCCA TCGCAGAGAC AGTGCAGATC
    CAAGTGAACC GTATCAGTAT GCATGATTAT GAAGCTCTCC ATTATGATAA GGAGCAGCTC
    AAAGAAGCCG CGATTCCCAT GGGGATAATT ACAGTTCCTG GGAACAGTGA TTTGGAGACG
    TGTCGGCAGC ATATCGAGAG GTTGCATGAG GAGGGGGATG GGATGAATAC AGACACACTG
    CACATGCCAA AGCTTTCACC AAAATGGTGC TTCCTGGACT GTACAACTGC AGATCGTTTC
    TACCGAATAG ATCGTGGTCA GGAGCATCTA AACTATGTGT CGGAGATCTC CCAGGATGAG
    CTTTTCATCT TGGACCCGGA GCTGGTCACC AAGGAGACGG TGGGCACGTC GCCCGGAATG
    CCGGGGGCGA TGGAAACGGT TGTTGGGGGA TGTGGATGTT CCAACAGCAC AGACCAGTTC
    GCTTTTCCTG CCTGCTCTCC AGGGCCTTCT CCACTCTGCA GGGTTAATGA AACACATGCT
    GAAAGCCAAC CGGAAAGTGT GAGTGAAGAA ATCGACATGC ATGTGCAAAG TGATCCAGAA
    AGTCCAACAA ATCTAGGCGT GTACAGGGTT CATGATACAC ATGCTGAAAG CCAACTGGAA
    AGCTTGAGTC CAGAAAGTGA CTCTCATGTG CAAAGTGATC CAGGAAGTCC CACAAAATCA
    GGCCTGTACA GGAGGGGAGC AAAAAATAAG ATTTCCAGTC GTTCAAACAC ACTTGAGGAG
    GACTCAGTGA ATGTGCAGAG GTTAGACACG GTCTGGAATA AGAGTGATCA AGATTCAACC
    AATTTGCTCT TTGATGCTGT GAAGTCTGGC AACTTGGAAA AGTTGGAACA GATGCATGAA
    GAAGGAGCGG ATCTCTCGGT CCAGGACCAT TTAGGTTGCA CCATCCTCCA TCAGGCAGTC
    ATTTTAGGTC ACAAAGAGAT TGTGAAGTTT ATCACTGAAA ACGCTCCTGC CTGCATACTG
    GATGTGACGG AGAAATCAAC AGGAGAGACA GCTCTGCATA AGGCAGCTGC GTTGTGCCAT
    AGAACAATTT GCTGTTATCT GGTGGATGCT GGTGCATCGC TCATGAAGAC AGATCTACAG
    GGTGAAACTC CAAAGATGTA CGCAGAGAGA ACTCGAGACT ACGAGCTAGC TGAATATTTG
    GAAAACCAAC AACACCATCA AATGATCCAG AGAGATGACC ATGAGACGGC AGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005174346.1
    CDS300..4196
    Translation

    Target ORF information:

    RefSeq Version XM_005174289.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio diacylglycerol kinase, zeta b (dgkzb), transcript variant X3, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005174289.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    3181
    3241
    3301
    3361
    3421
    3481
    3541
    3601
    3661
    3721
    3781
    3841
    ATGGACACTT TTTTCCGACG GCATTTTAAG AGAAAGGAAG CAGGGGACGG TGTGCATGAT 
    GACTCCTGTC GACACCGGAG ACCGAGCATT GCAGTGCCAA CCAGCCGTGC CCGACGGCGC
    TCCAGTGTCG GCCTCCCATC CAGTGCATTA ACCCAGCGGC GCCGATCCAG CGTCCAACTT
    CAGGCATTGC CCCGAATCAA CAGCAGTCAA CCGGTACATC AAAAGAGCAG CGGTAGACGG
    AGGTCAAGTA CCACCACCCT GAAAGCCAAT CCTCGCTTTG CTGTGCGCCG TAAGAAAGTA
    GGCAAGCTTC GCAATATAGA CACACACCTC CTAGGCCCAT CCATGCTGCT AGCCAGTCTG
    ATCCAAATGA CTCAAGAAGA GGAAGACGAT GAAGAAGGTC GCAGTCCGCT CCCAGGGCAA
    GTGGAGCTCA AGGGAAGCAG CTCGAAGTTC AGGAGTCACA CAGATGTGCT GCTTTCTGAA
    GGTGACAGTG ATAGTGATGA CTCCAGCGAG CATTCAGAGG AACGTCAATA CTCAAGGATA
    CGCAAAGAGT GTCTGGAGGA GGCTGATGGG TCAGACTGGG GGGTCAAGGA GTCTGGTGTT
    TTATGCCAGC AGGACTGCCT TCCTCTGTCT GTGGTGGCGG AGCGAATCCA GGCCCATCCG
    CTGATTCGAG TCCCGCGCTG CCTCAGGAGG AACTCGTCCC ATTATGGCCA TGCGGATCCA
    GGACCGCATG TGCGCTACTG CCGCAGCAGC CAAAGGAGGC GTCGTAGGAG GGTCTCCACA
    ATCTCCAAAG CAGGAAGCCC ATGGCCTGGA AGAGGCCTGC CGCTGCCTAA CCGAAGAAGC
    TCCTGTTACA GGGGTCCGGC GACTTTCAAT CCTCTCCTGG GGGCTTATTA TGACCCCCGA
    TTGGAGTCCT GGAGTGGATT CTTGTCGAAA GCCATCGCCA AAGCTGGTGT GCAGGATAAC
    GCTGCTCAAT CCGGCACAAT AACACCTGCT AAAAGTGAAC CCAACAAAAA GATATGCAAC
    ACCGTGGATT GGACCGAAAA TGCCCAGTTG GGTCAACACG TGTGGTTTGA GACCAGTCCG
    GCAGGAGATT TCTGTTATGT GGGGGAGTCA AACTGTATCG CAAAGTCCCA GCAAAAGTCT
    CTTCCCCGCC ACAAATGTGC AGCTTGCAAA ATATCAGTTC ACACAGAGTG CATGAATCAG
    CTGGACAAGA TTAACTTCAG GTGTAAGCCA ACTTTTAAAG ATCCAGGGAC GAAAAATGTC
    AGAGAAACTC CAAATATGAA CCATCACTGG GTCCATAGGA GAAGGCAAGA TGGAAAATGC
    CGGCAATGTG GAAAGGGTTT CCAGCAGAAA TTCTCCTTCC ACAGTAAAGA CATTGTGGCC
    ATCAGTTGCT CATGGTGTAA ACAGGCTTAT CACAACAAGG TGAACTGTTT CATGATGCAG
    AGAATCGATG AGCCGTGTTC CCTTGGCGCT CACGCAGCTG TGATCATCCC TCCAACCTGG
    ATTATCAGAA TGCGGAGGCA GCAGGCATCA CTGAAGAGCA GTAAAAGAAG GAAGAGAACG
    TCTCTGAAGG CCAAACCCAG CAAGAAATTA GAGCAGGACG GCAAATGGAG AAACTTCGTG
    GTGAGAGCGA TTCCTTCCCA GCACATGAAG CCTCTGCTGG TGTTCGTCAA TCCGAAGAGT
    GGTGGAAACC AGGGAACGAA AATCATCCGT ACTTTCATGT GGTACCTGAA CCCCCGGCAA
    GTGTTCGACC TGACTCAGGG AGGTCCGAGA GAGGGGTTAG AAATGTACAG CAAAGTGCCC
    AACCTGCGCA TCCTTGTTTG TGGTGGCGAT GGAACGGTTG GCTGGATTCT GTCAGTGCTG
    GATGAGCTTC AGTTGAACCC TCAACCTGCT GTGGCCGTTC TTCCCCTTGG GACAGGAAAT
    GACCTGGCCA GGACCCTCAA CTGGGGCGGG GGCTACACAG ATGAACCAGT ATCCAAGATC
    CTTTCCCATG TTGAAGACGG CATCATCGTC CAGCTGGATC GCTGGAACCT GAGTGTGGAA
    GCTAATTTAG AGGCCAGCGA TGAGGACAAA GATGAACAAC AGACCGACAA GCTTCCCATT
    GACGTCTTCA ACAATTACTT CAGCCTCGGG TTTGACGCAC ATGTGACTTT GGAGTTTCAC
    GAATCCCGAG AGGCTAAACC GGAACGCTTC AACAGTCGAC TTCGCAATAA GATGTTTTAC
    GCAGGGACAG CATTCTCAGA CTTCCTGATG GGCAGCTCTA AAGATCTGTC CAAACACATC
    AAGGTTGTGT GTGACGGTAC TGACCTCACC AGTAAAATTC AAGATATGAA AGTGCTGTGC
    CTGCTGTTCC TCAACATCCC CAGATATTGC GCTGGTACCA TGCCATGGGG GAACCCAAGT
    GAGAACAATG ACTTCGGGCC ACAGAAACAC GACGACGGGC TTATTGAGGT CATCGGCTTC
    ACCATGACCT CTATGGCAAC TCTTCAGGTT GGAGGACATG GCGAGAGGCT TCATCAGTGT
    AAGGAGGTGA CCCTGACTAC TTTTAAGCCC ATTCCTGTAC AGGTGGATGG GGAACCCTGT
    AGACTGGCTC CATCCGTCAT ACACATCACT CGAAGGAACC AAGCCAACAT GCTGCAGAAA
    ACCAAGAGGA GGATCTCTCT ACCACAACTT CAGCAGCCCA TCGCAGAGAC AGTGCAGATC
    CAAGTGAACC GTATCAGTAT GCATGATTAT GAAGCTCTCC ATTATGATAA GGAGCAGCTC
    AAAGAAGCCG CGATTCCCAT GGGGATAATT ACAGTTCCTG GGAACAGTGA TTTGGAGACG
    TGTCGGCAGC ATATCGAGAG GTTGCATGAG GAGGGGGATG GGATGAATAC AGACACACTG
    CACATGCCAA AGCTTTCACC AAAATGGTGC TTCCTGGACT GTACAACTGC AGATCGTTTC
    TACCGAATAG ATCGTGGTCA GGAGCATCTA AACTATGTGT CGGAGATCTC CCAGGATGAG
    CTTTTCATCT TGGACCCGGA GCTGGTCACC AAGGAGACGG TGGGCACGTC GCCCGGAATG
    CCGGGGGCGA TGGAAACGGT TGTTGGGGGA TGTGGATGTT CCAACAGCAC AGACCAGTTC
    GCTTTTCCTG CCTGCTCTCC AGGGCCTTCT CCACTCTGCA GGGTTAATGA AACACATGCT
    GAAAGCCAAC CGGAAAGTGT GAGTGAAGAA ATCGACATGC ATGTGCAAAG TGATCCAGAA
    AGTCCAACAA ATCTAGGCGT GTACAGGGTT CATGATACAC ATGCTGAAAG CCAACTGGAA
    AGCTTGAGTC CAGAAAGTGA CTCTCATGTG CAAAGTGATC CAGGAAGTCC CACAAAATCA
    GGCCTGTACA GGAGGGGAGC AAAAAATAAG ATTTCCAGTC GTTCAAACAC ACTTGAGGAG
    GACTCAGTGA ATGTGCAGAG GTTAGACACG GTCTGGAATA AGAGTGATCA AGATTCAACC
    AATTTGCTCT TTGATGCTGT GAAGTCTGGC AACTTGGAAA AGTTGGAACA GATGCATGAA
    GAAGGAGCGG ATCTCTCGGT CCAGGACCAT TTAGGTTGCA CCATCCTCCA TCAGGCAGTC
    ATTTTAGGTC ACAAAGAGAT TGTGAAGTTT ATCACTGAAA ACGCTCCTGC CTGCATACTG
    GATGTGACGG AGAAATCAAC AGGAGAGACA GCTCTGCATA AGGCAGCTGC GTTGTGCCAT
    AGAACAATTT GCTGTTATCT GGTGGATGCT GGTGCATCGC TCATGAAGAC AGATCTACAG
    GGTGAAACTC CAAAGATGTA CGCAGAGAGA ACTCGAGACT ACGAGCTAGC TGAATATTTG
    GAAAACCAAC AACACCATCA AATGATCCAG AGAGATGACC ATGAGACGGC AGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa29155
    Clone ID Related Accession (Same CDS sequence) XM_005174290.3 , XM_005174290.4
    Accession Version XM_005174290.3 Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3177bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2016-06-23
    Organism Danio rerio(Zebrafish)
    Product diacylglycerol kinase zeta isoform X4
    Comment MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005174290.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTTTGAGA TGGAGCGCCT TGAGGGAGAG GTGGAGGAGC TGTCCCGACC GTTGGAGGCT 
    CTGCTGGATG GTGAAGAGCC TGGAGGTCCT GATTCTGCCT CCTCGTCCTG TTCTGACCTG
    TCCAACACAA AGCCTGTGGT TCAGCCCGGT CCCAACAACA ACAGCAAGAG CTTCCCTGGA
    CTGCGGCTCT TTGGTAGAAG GAAAGCCATC GCCAAAGCTG GTGTGCAGGA TAACGCTGCT
    CAATCCGGCA CAATAACACC TGCTAAAAGT GAACCCAACA AAAAGATATG CAACACCGTG
    GATTGGACCG AAAATGCCCA GTTGGGTCAA CACGTGTGGT TTGAGACCAG TCCGGCAGGA
    GATTTCTGTT ATGTGGGGGA GTCAAACTGT ATCGCAAAGT CCCAGCAAAA GTCTCTTCCC
    CGCCACAAAT GTGCAGCTTG CAAAATATCA GTTCACACAG AGTGCATGAA TCAGCTGGAC
    AAGATTAACT TCAGGTGTAA GCCAACTTTT AAAGATCCAG GGACGAAAAA TGTCAGAGAA
    ACTCCAAATA TGAACCATCA CTGGGTCCAT AGGAGAAGGC AAGATGGAAA ATGCCGGCAA
    TGTGGAAAGG GTTTCCAGCA GAAATTCTCC TTCCACAGTA AAGACATTGT GGCCATCAGT
    TGCTCATGGT GTAAACAGGC TTATCACAAC AAGGTGAACT GTTTCATGAT GCAGAGAATC
    GATGAGCCGT GTTCCCTTGG CGCTCACGCA GCTGTGATCA TCCCTCCAAC CTGGATTATC
    AGAATGCGGA GGCAGCAGGC ATCACTGAAG AGCAGTAAAA GAAGGAAGAG AACGTCTCTG
    AAGGCCAAAC CCAGCAAGAA ATTAGAGCAG GACGGCAAAT GGAGAAACTT CGTGGTGAGA
    GCGATTCCTT CCCAGCACAT GAAGCCTCTG CTGGTGTTCG TCAATCCGAA GAGTGGTGGA
    AACCAGGGAA CGAAAATCAT CCGTACTTTC ATGTGGTACC TGAACCCCCG GCAAGTGTTC
    GACCTGACTC AGGGAGGTCC GAGAGAGGGG TTAGAAATGT ACAGCAAAGT GCCCAACCTG
    CGCATCCTTG TTTGTGGTGG CGATGGAACG GTTGGCTGGA TTCTGTCAGT GCTGGATGAG
    CTTCAGTTGA ACCCTCAACC TGCTGTGGCC GTTCTTCCCC TTGGGACAGG AAATGACCTG
    GCCAGGACCC TCAACTGGGG CGGGGGCTAC ACAGATGAAC CAGTATCCAA GATCCTTTCC
    CATGTTGAAG ACGGCATCAT CGTCCAGCTG GATCGCTGGA ACCTGAGTGT GGAAGCTAAT
    TTAGAGGCCA GCGATGAGGA CAAAGATGAA CAACAGACCG ACAAGCTTCC CATTGACGTC
    TTCAACAATT ACTTCAGCCT CGGGTTTGAC GCACATGTGA CTTTGGAGTT TCACGAATCC
    CGAGAGGCTA AACCGGAACG CTTCAACAGT CGACTTCGCA ATAAGATGTT TTACGCAGGG
    ACAGCATTCT CAGACTTCCT GATGGGCAGC TCTAAAGATC TGTCCAAACA CATCAAGGTT
    GTGTGTGACG GTACTGACCT CACCAGTAAA ATTCAAGATA TGAAAGTGCT GTGCCTGCTG
    TTCCTCAACA TCCCCAGATA TTGCGCTGGT ACCATGCCAT GGGGGAACCC AAGTGAGAAC
    AATGACTTCG GGCCACAGAA ACACGACGAC GGGCTTATTG AGGTCATCGG CTTCACCATG
    ACCTCTATGG CAACTCTTCA GGTTGGAGGA CATGGCGAGA GGCTTCATCA GTGTAAGGAG
    GTGACCCTGA CTACTTTTAA GCCCATTCCT GTACAGGTGG ATGGGGAACC CTGTAGACTG
    GCTCCATCCG TCATACACAT CACTCGAAGG AACCAAGCCA ACATGCTGCA GAAAACCAAG
    AGGAGGATCT CTCTACCACA ACTGTATGAT CAGCAGCCCA TCGCAGAGAC AGTGCAGATC
    CAAGTGAACC GTATCAGTAT GCATGATTAT GAAGCTCTCC ATTATGATAA GGAGCAGCTC
    AAAGAAGCCG CGATTCCCAT GGGGATAATT ACAGTTCCTG GGAACAGTGA TTTGGAGACG
    TGTCGGCAGC ATATCGAGAG GTTGCATGAG GAGGGGGATG GGATGAATAC AGACACACTG
    CACATGCCAA AGCTTTCACC AAAATGGTGC TTCCTGGACT GTACAACTGC AGATCGTTTC
    TACCGAATAG ATCGTGGTCA GGAGCATCTA AACTATGTGT CGGAGATCTC CCAGGATGAG
    CTTTTCATCT TGGACCCGGA GCTGGTCACC AAGGAGACGG TGGGCACGTC GCCCGGAATG
    CCGGGGGCGA TGGAAACGGT TGTTGGGGGA TGTGGATGTT CCAACAGCAC AGACCAGTTC
    GCTTTTCCTG CCTGCTCTCC AGGGCCTTCT CCACTCTGCA GGGTTAATGA AACACATGCT
    GAAAGCCAAC CGGAAAGTGT GAGTGAAGAA ATCGACATGC ATGTGCAAAG TGATCCAGAA
    AGTCCAACAA ATCTAGGCGT GTACAGGGTT CATGATACAC ATGCTGAAAG CCAACTGGAA
    AGCTTGAGTC CAGAAAGTGA CTCTCATGTG CAAAGTGATC CAGGAAGTCC CACAAAATCA
    GGCCTGTACA GGAGGGGAGC AAAAAATAAG ATTTCCAGTC GTTCAAACAC ACTTGAGGAG
    GACTCAGTGA ATGTGCAGAG GTTAGACACG GTCTGGAATA AGAGTGATCA AGATTCAACC
    AATTTGCTCT TTGATGCTGT GAAGTCTGGC AACTTGGAAA AGTTGGAACA GATGCATGAA
    GAAGGAGCGG ATCTCTCGGT CCAGGACCAT TTAGGTTGCA CCATCCTCCA TCAGGCAGTC
    ATTTTAGGTC ACAAAGAGAT TGTGAAGTTT ATCACTGAAA ACGCTCCTGC CTGCATACTG
    GATGTGACGG AGAAATCAAC AGGAGAGACA GCTCTGCATA AGGCAGCTGC GTTGTGCCAT
    AGAACAATTT GCTGTTATCT GGTGGATGCT GGTGCATCGC TCATGAAGAC AGATCTACAG
    GGTGAAACTC CAAAGATGTA CGCAGAGAGA ACTCGAGACT ACGAGCTAGC TGAATATTTG
    GAAAACCAAC AACACCATCA AATGATCCAG AGAGATGACC ATGAGACGGC AGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005174347.1
    CDS362..3538
    Translation

    Target ORF information:

    RefSeq Version XM_005174290.3
    Organism Danio rerio(Zebrafish)
    Definition Danio rerio diacylglycerol kinase, zeta b (dgkzb), transcript variant X4, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005174290.3

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTTTGAGA TGGAGCGCCT TGAGGGAGAG GTGGAGGAGC TGTCCCGACC GTTGGAGGCT 
    CTGCTGGATG GTGAAGAGCC TGGAGGTCCT GATTCTGCCT CCTCGTCCTG TTCTGACCTG
    TCCAACACAA AGCCTGTGGT TCAGCCCGGT CCCAACAACA ACAGCAAGAG CTTCCCTGGA
    CTGCGGCTCT TTGGTAGAAG GAAAGCCATC GCCAAAGCTG GTGTGCAGGA TAACGCTGCT
    CAATCCGGCA CAATAACACC TGCTAAAAGT GAACCCAACA AAAAGATATG CAACACCGTG
    GATTGGACCG AAAATGCCCA GTTGGGTCAA CACGTGTGGT TTGAGACCAG TCCGGCAGGA
    GATTTCTGTT ATGTGGGGGA GTCAAACTGT ATCGCAAAGT CCCAGCAAAA GTCTCTTCCC
    CGCCACAAAT GTGCAGCTTG CAAAATATCA GTTCACACAG AGTGCATGAA TCAGCTGGAC
    AAGATTAACT TCAGGTGTAA GCCAACTTTT AAAGATCCAG GGACGAAAAA TGTCAGAGAA
    ACTCCAAATA TGAACCATCA CTGGGTCCAT AGGAGAAGGC AAGATGGAAA ATGCCGGCAA
    TGTGGAAAGG GTTTCCAGCA GAAATTCTCC TTCCACAGTA AAGACATTGT GGCCATCAGT
    TGCTCATGGT GTAAACAGGC TTATCACAAC AAGGTGAACT GTTTCATGAT GCAGAGAATC
    GATGAGCCGT GTTCCCTTGG CGCTCACGCA GCTGTGATCA TCCCTCCAAC CTGGATTATC
    AGAATGCGGA GGCAGCAGGC ATCACTGAAG AGCAGTAAAA GAAGGAAGAG AACGTCTCTG
    AAGGCCAAAC CCAGCAAGAA ATTAGAGCAG GACGGCAAAT GGAGAAACTT CGTGGTGAGA
    GCGATTCCTT CCCAGCACAT GAAGCCTCTG CTGGTGTTCG TCAATCCGAA GAGTGGTGGA
    AACCAGGGAA CGAAAATCAT CCGTACTTTC ATGTGGTACC TGAACCCCCG GCAAGTGTTC
    GACCTGACTC AGGGAGGTCC GAGAGAGGGG TTAGAAATGT ACAGCAAAGT GCCCAACCTG
    CGCATCCTTG TTTGTGGTGG CGATGGAACG GTTGGCTGGA TTCTGTCAGT GCTGGATGAG
    CTTCAGTTGA ACCCTCAACC TGCTGTGGCC GTTCTTCCCC TTGGGACAGG AAATGACCTG
    GCCAGGACCC TCAACTGGGG CGGGGGCTAC ACAGATGAAC CAGTATCCAA GATCCTTTCC
    CATGTTGAAG ACGGCATCAT CGTCCAGCTG GATCGCTGGA ACCTGAGTGT GGAAGCTAAT
    TTAGAGGCCA GCGATGAGGA CAAAGATGAA CAACAGACCG ACAAGCTTCC CATTGACGTC
    TTCAACAATT ACTTCAGCCT CGGGTTTGAC GCACATGTGA CTTTGGAGTT TCACGAATCC
    CGAGAGGCTA AACCGGAACG CTTCAACAGT CGACTTCGCA ATAAGATGTT TTACGCAGGG
    ACAGCATTCT CAGACTTCCT GATGGGCAGC TCTAAAGATC TGTCCAAACA CATCAAGGTT
    GTGTGTGACG GTACTGACCT CACCAGTAAA ATTCAAGATA TGAAAGTGCT GTGCCTGCTG
    TTCCTCAACA TCCCCAGATA TTGCGCTGGT ACCATGCCAT GGGGGAACCC AAGTGAGAAC
    AATGACTTCG GGCCACAGAA ACACGACGAC GGGCTTATTG AGGTCATCGG CTTCACCATG
    ACCTCTATGG CAACTCTTCA GGTTGGAGGA CATGGCGAGA GGCTTCATCA GTGTAAGGAG
    GTGACCCTGA CTACTTTTAA GCCCATTCCT GTACAGGTGG ATGGGGAACC CTGTAGACTG
    GCTCCATCCG TCATACACAT CACTCGAAGG AACCAAGCCA ACATGCTGCA GAAAACCAAG
    AGGAGGATCT CTCTACCACA ACTGTATGAT CAGCAGCCCA TCGCAGAGAC AGTGCAGATC
    CAAGTGAACC GTATCAGTAT GCATGATTAT GAAGCTCTCC ATTATGATAA GGAGCAGCTC
    AAAGAAGCCG CGATTCCCAT GGGGATAATT ACAGTTCCTG GGAACAGTGA TTTGGAGACG
    TGTCGGCAGC ATATCGAGAG GTTGCATGAG GAGGGGGATG GGATGAATAC AGACACACTG
    CACATGCCAA AGCTTTCACC AAAATGGTGC TTCCTGGACT GTACAACTGC AGATCGTTTC
    TACCGAATAG ATCGTGGTCA GGAGCATCTA AACTATGTGT CGGAGATCTC CCAGGATGAG
    CTTTTCATCT TGGACCCGGA GCTGGTCACC AAGGAGACGG TGGGCACGTC GCCCGGAATG
    CCGGGGGCGA TGGAAACGGT TGTTGGGGGA TGTGGATGTT CCAACAGCAC AGACCAGTTC
    GCTTTTCCTG CCTGCTCTCC AGGGCCTTCT CCACTCTGCA GGGTTAATGA AACACATGCT
    GAAAGCCAAC CGGAAAGTGT GAGTGAAGAA ATCGACATGC ATGTGCAAAG TGATCCAGAA
    AGTCCAACAA ATCTAGGCGT GTACAGGGTT CATGATACAC ATGCTGAAAG CCAACTGGAA
    AGCTTGAGTC CAGAAAGTGA CTCTCATGTG CAAAGTGATC CAGGAAGTCC CACAAAATCA
    GGCCTGTACA GGAGGGGAGC AAAAAATAAG ATTTCCAGTC GTTCAAACAC ACTTGAGGAG
    GACTCAGTGA ATGTGCAGAG GTTAGACACG GTCTGGAATA AGAGTGATCA AGATTCAACC
    AATTTGCTCT TTGATGCTGT GAAGTCTGGC AACTTGGAAA AGTTGGAACA GATGCATGAA
    GAAGGAGCGG ATCTCTCGGT CCAGGACCAT TTAGGTTGCA CCATCCTCCA TCAGGCAGTC
    ATTTTAGGTC ACAAAGAGAT TGTGAAGTTT ATCACTGAAA ACGCTCCTGC CTGCATACTG
    GATGTGACGG AGAAATCAAC AGGAGAGACA GCTCTGCATA AGGCAGCTGC GTTGTGCCAT
    AGAACAATTT GCTGTTATCT GGTGGATGCT GGTGCATCGC TCATGAAGAC AGATCTACAG
    GGTGAAACTC CAAAGATGTA CGCAGAGAGA ACTCGAGACT ACGAGCTAGC TGAATATTTG
    GAAAACCAAC AACACCATCA AATGATCCAG AGAGATGACC ATGAGACGGC AGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    CloneID ODa29155
    Clone ID Related Accession (Same CDS sequence) XM_005174290.3 , XM_005174290.4
    Accession Version XM_005174290.4 Latest version! Documents for ORF clone product in default vector
    Sequence Information ORF Nucleotide Sequence (Length: 3177bp)
    Protein sequence
    SNP
    Vector pcDNA3.1-C-(k)DYK or customized vector User Manual
    Clone information Clone Map MSDS
    Tag on pcDNA3.1+/C-(K)DYK C terminal DYKDDDDK tags
    ORF Insert Method CloneEZ™ Seamless cloning technology
    Insert Structure linear
    Update Date 2017-06-14
    Organism Danio rerio(zebrafish)
    Product diacylglycerol kinase zeta isoform X5
    Comment Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007136.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005174290.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTTTGAGA TGGAGCGCCT TGAGGGAGAG GTGGAGGAGC TGTCCCGACC GTTGGAGGCT 
    CTGCTGGATG GTGAAGAGCC TGGAGGTCCT GATTCTGCCT CCTCGTCCTG TTCTGACCTG
    TCCAACACAA AGCCTGTGGT TCAGCCCGGT CCCAACAACA ACAGCAAGAG CTTCCCTGGA
    CTGCGGCTCT TTGGTAGAAG GAAAGCCATC GCCAAAGCTG GTGTGCAGGA TAACGCTGCT
    CAATCCGGCA CAATAACACC TGCTAAAAGT GAACCCAACA AAAAGATATG CAACACCGTG
    GATTGGACCG AAAATGCCCA GTTGGGTCAA CACGTGTGGT TTGAGACCAG TCCGGCAGGA
    GATTTCTGTT ATGTGGGGGA GTCAAACTGT ATCGCAAAGT CCCAGCAAAA GTCTCTTCCC
    CGCCACAAAT GTGCAGCTTG CAAAATATCA GTTCACACAG AGTGCATGAA TCAGCTGGAC
    AAGATTAACT TCAGGTGTAA GCCAACTTTT AAAGATCCAG GGACGAAAAA TGTCAGAGAA
    ACTCCAAATA TGAACCATCA CTGGGTCCAT AGGAGAAGGC AAGATGGAAA ATGCCGGCAA
    TGTGGAAAGG GTTTCCAGCA GAAATTCTCC TTCCACAGTA AAGACATTGT GGCCATCAGT
    TGCTCATGGT GTAAACAGGC TTATCACAAC AAGGTGAACT GTTTCATGAT GCAGAGAATC
    GATGAGCCGT GTTCCCTTGG CGCTCACGCA GCTGTGATCA TCCCTCCAAC CTGGATTATC
    AGAATGCGGA GGCAGCAGGC ATCACTGAAG AGCAGTAAAA GAAGGAAGAG AACGTCTCTG
    AAGGCCAAAC CCAGCAAGAA ATTAGAGCAG GACGGCAAAT GGAGAAACTT CGTGGTGAGA
    GCGATTCCTT CCCAGCACAT GAAGCCTCTG CTGGTGTTCG TCAATCCGAA GAGTGGTGGA
    AACCAGGGAA CGAAAATCAT CCGTACTTTC ATGTGGTACC TGAACCCCCG GCAAGTGTTC
    GACCTGACTC AGGGAGGTCC GAGAGAGGGG TTAGAAATGT ACAGCAAAGT GCCCAACCTG
    CGCATCCTTG TTTGTGGTGG CGATGGAACG GTTGGCTGGA TTCTGTCAGT GCTGGATGAG
    CTTCAGTTGA ACCCTCAACC TGCTGTGGCC GTTCTTCCCC TTGGGACAGG AAATGACCTG
    GCCAGGACCC TCAACTGGGG CGGGGGCTAC ACAGATGAAC CAGTATCCAA GATCCTTTCC
    CATGTTGAAG ACGGCATCAT CGTCCAGCTG GATCGCTGGA ACCTGAGTGT GGAAGCTAAT
    TTAGAGGCCA GCGATGAGGA CAAAGATGAA CAACAGACCG ACAAGCTTCC CATTGACGTC
    TTCAACAATT ACTTCAGCCT CGGGTTTGAC GCACATGTGA CTTTGGAGTT TCACGAATCC
    CGAGAGGCTA AACCGGAACG CTTCAACAGT CGACTTCGCA ATAAGATGTT TTACGCAGGG
    ACAGCATTCT CAGACTTCCT GATGGGCAGC TCTAAAGATC TGTCCAAACA CATCAAGGTT
    GTGTGTGACG GTACTGACCT CACCAGTAAA ATTCAAGATA TGAAAGTGCT GTGCCTGCTG
    TTCCTCAACA TCCCCAGATA TTGCGCTGGT ACCATGCCAT GGGGGAACCC AAGTGAGAAC
    AATGACTTCG GGCCACAGAA ACACGACGAC GGGCTTATTG AGGTCATCGG CTTCACCATG
    ACCTCTATGG CAACTCTTCA GGTTGGAGGA CATGGCGAGA GGCTTCATCA GTGTAAGGAG
    GTGACCCTGA CTACTTTTAA GCCCATTCCT GTACAGGTGG ATGGGGAACC CTGTAGACTG
    GCTCCATCCG TCATACACAT CACTCGAAGG AACCAAGCCA ACATGCTGCA GAAAACCAAG
    AGGAGGATCT CTCTACCACA ACTGTATGAT CAGCAGCCCA TCGCAGAGAC AGTGCAGATC
    CAAGTGAACC GTATCAGTAT GCATGATTAT GAAGCTCTCC ATTATGATAA GGAGCAGCTC
    AAAGAAGCCG CGATTCCCAT GGGGATAATT ACAGTTCCTG GGAACAGTGA TTTGGAGACG
    TGTCGGCAGC ATATCGAGAG GTTGCATGAG GAGGGGGATG GGATGAATAC AGACACACTG
    CACATGCCAA AGCTTTCACC AAAATGGTGC TTCCTGGACT GTACAACTGC AGATCGTTTC
    TACCGAATAG ATCGTGGTCA GGAGCATCTA AACTATGTGT CGGAGATCTC CCAGGATGAG
    CTTTTCATCT TGGACCCGGA GCTGGTCACC AAGGAGACGG TGGGCACGTC GCCCGGAATG
    CCGGGGGCGA TGGAAACGGT TGTTGGGGGA TGTGGATGTT CCAACAGCAC AGACCAGTTC
    GCTTTTCCTG CCTGCTCTCC AGGGCCTTCT CCACTCTGCA GGGTTAATGA AACACATGCT
    GAAAGCCAAC CGGAAAGTGT GAGTGAAGAA ATCGACATGC ATGTGCAAAG TGATCCAGAA
    AGTCCAACAA ATCTAGGCGT GTACAGGGTT CATGATACAC ATGCTGAAAG CCAACTGGAA
    AGCTTGAGTC CAGAAAGTGA CTCTCATGTG CAAAGTGATC CAGGAAGTCC CACAAAATCA
    GGCCTGTACA GGAGGGGAGC AAAAAATAAG ATTTCCAGTC GTTCAAACAC ACTTGAGGAG
    GACTCAGTGA ATGTGCAGAG GTTAGACACG GTCTGGAATA AGAGTGATCA AGATTCAACC
    AATTTGCTCT TTGATGCTGT GAAGTCTGGC AACTTGGAAA AGTTGGAACA GATGCATGAA
    GAAGGAGCGG ATCTCTCGGT CCAGGACCAT TTAGGTTGCA CCATCCTCCA TCAGGCAGTC
    ATTTTAGGTC ACAAAGAGAT TGTGAAGTTT ATCACTGAAA ACGCTCCTGC CTGCATACTG
    GATGTGACGG AGAAATCAAC AGGAGAGACA GCTCTGCATA AGGCAGCTGC GTTGTGCCAT
    AGAACAATTT GCTGTTATCT GGTGGATGCT GGTGCATCGC TCATGAAGAC AGATCTACAG
    GGTGAAACTC CAAAGATGTA CGCAGAGAGA ACTCGAGACT ACGAGCTAGC TGAATATTTG
    GAAAACCAAC AACACCATCA AATGATCCAG AGAGATGACC ATGAGACGGC AGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.

    RefSeq XP_005174347.1
    CDS362..3538
    Translation

    Target ORF information:

    RefSeq Version XM_005174290.4
    Organism Danio rerio(zebrafish)
    Definition Danio rerio diacylglycerol kinase, zeta b (dgkzb), transcript variant X5, mRNA.

    Target ORF information:

    Epitope DYKDDDDK
    Bacterial selection AMPR
    Mammalian selection NeoR
    Vector pcDNA3.1+/C-(K)DYK
    XM_005174290.4

    ORF Insert Sequence:

    1
    61
    121
    181
    241
    301
    361
    421
    481
    541
    601
    661
    721
    781
    841
    901
    961
    1021
    1081
    1141
    1201
    1261
    1321
    1381
    1441
    1501
    1561
    1621
    1681
    1741
    1801
    1861
    1921
    1981
    2041
    2101
    2161
    2221
    2281
    2341
    2401
    2461
    2521
    2581
    2641
    2701
    2761
    2821
    2881
    2941
    3001
    3061
    3121
    ATGTTTGAGA TGGAGCGCCT TGAGGGAGAG GTGGAGGAGC TGTCCCGACC GTTGGAGGCT 
    CTGCTGGATG GTGAAGAGCC TGGAGGTCCT GATTCTGCCT CCTCGTCCTG TTCTGACCTG
    TCCAACACAA AGCCTGTGGT TCAGCCCGGT CCCAACAACA ACAGCAAGAG CTTCCCTGGA
    CTGCGGCTCT TTGGTAGAAG GAAAGCCATC GCCAAAGCTG GTGTGCAGGA TAACGCTGCT
    CAATCCGGCA CAATAACACC TGCTAAAAGT GAACCCAACA AAAAGATATG CAACACCGTG
    GATTGGACCG AAAATGCCCA GTTGGGTCAA CACGTGTGGT TTGAGACCAG TCCGGCAGGA
    GATTTCTGTT ATGTGGGGGA GTCAAACTGT ATCGCAAAGT CCCAGCAAAA GTCTCTTCCC
    CGCCACAAAT GTGCAGCTTG CAAAATATCA GTTCACACAG AGTGCATGAA TCAGCTGGAC
    AAGATTAACT TCAGGTGTAA GCCAACTTTT AAAGATCCAG GGACGAAAAA TGTCAGAGAA
    ACTCCAAATA TGAACCATCA CTGGGTCCAT AGGAGAAGGC AAGATGGAAA ATGCCGGCAA
    TGTGGAAAGG GTTTCCAGCA GAAATTCTCC TTCCACAGTA AAGACATTGT GGCCATCAGT
    TGCTCATGGT GTAAACAGGC TTATCACAAC AAGGTGAACT GTTTCATGAT GCAGAGAATC
    GATGAGCCGT GTTCCCTTGG CGCTCACGCA GCTGTGATCA TCCCTCCAAC CTGGATTATC
    AGAATGCGGA GGCAGCAGGC ATCACTGAAG AGCAGTAAAA GAAGGAAGAG AACGTCTCTG
    AAGGCCAAAC CCAGCAAGAA ATTAGAGCAG GACGGCAAAT GGAGAAACTT CGTGGTGAGA
    GCGATTCCTT CCCAGCACAT GAAGCCTCTG CTGGTGTTCG TCAATCCGAA GAGTGGTGGA
    AACCAGGGAA CGAAAATCAT CCGTACTTTC ATGTGGTACC TGAACCCCCG GCAAGTGTTC
    GACCTGACTC AGGGAGGTCC GAGAGAGGGG TTAGAAATGT ACAGCAAAGT GCCCAACCTG
    CGCATCCTTG TTTGTGGTGG CGATGGAACG GTTGGCTGGA TTCTGTCAGT GCTGGATGAG
    CTTCAGTTGA ACCCTCAACC TGCTGTGGCC GTTCTTCCCC TTGGGACAGG AAATGACCTG
    GCCAGGACCC TCAACTGGGG CGGGGGCTAC ACAGATGAAC CAGTATCCAA GATCCTTTCC
    CATGTTGAAG ACGGCATCAT CGTCCAGCTG GATCGCTGGA ACCTGAGTGT GGAAGCTAAT
    TTAGAGGCCA GCGATGAGGA CAAAGATGAA CAACAGACCG ACAAGCTTCC CATTGACGTC
    TTCAACAATT ACTTCAGCCT CGGGTTTGAC GCACATGTGA CTTTGGAGTT TCACGAATCC
    CGAGAGGCTA AACCGGAACG CTTCAACAGT CGACTTCGCA ATAAGATGTT TTACGCAGGG
    ACAGCATTCT CAGACTTCCT GATGGGCAGC TCTAAAGATC TGTCCAAACA CATCAAGGTT
    GTGTGTGACG GTACTGACCT CACCAGTAAA ATTCAAGATA TGAAAGTGCT GTGCCTGCTG
    TTCCTCAACA TCCCCAGATA TTGCGCTGGT ACCATGCCAT GGGGGAACCC AAGTGAGAAC
    AATGACTTCG GGCCACAGAA ACACGACGAC GGGCTTATTG AGGTCATCGG CTTCACCATG
    ACCTCTATGG CAACTCTTCA GGTTGGAGGA CATGGCGAGA GGCTTCATCA GTGTAAGGAG
    GTGACCCTGA CTACTTTTAA GCCCATTCCT GTACAGGTGG ATGGGGAACC CTGTAGACTG
    GCTCCATCCG TCATACACAT CACTCGAAGG AACCAAGCCA ACATGCTGCA GAAAACCAAG
    AGGAGGATCT CTCTACCACA ACTGTATGAT CAGCAGCCCA TCGCAGAGAC AGTGCAGATC
    CAAGTGAACC GTATCAGTAT GCATGATTAT GAAGCTCTCC ATTATGATAA GGAGCAGCTC
    AAAGAAGCCG CGATTCCCAT GGGGATAATT ACAGTTCCTG GGAACAGTGA TTTGGAGACG
    TGTCGGCAGC ATATCGAGAG GTTGCATGAG GAGGGGGATG GGATGAATAC AGACACACTG
    CACATGCCAA AGCTTTCACC AAAATGGTGC TTCCTGGACT GTACAACTGC AGATCGTTTC
    TACCGAATAG ATCGTGGTCA GGAGCATCTA AACTATGTGT CGGAGATCTC CCAGGATGAG
    CTTTTCATCT TGGACCCGGA GCTGGTCACC AAGGAGACGG TGGGCACGTC GCCCGGAATG
    CCGGGGGCGA TGGAAACGGT TGTTGGGGGA TGTGGATGTT CCAACAGCAC AGACCAGTTC
    GCTTTTCCTG CCTGCTCTCC AGGGCCTTCT CCACTCTGCA GGGTTAATGA AACACATGCT
    GAAAGCCAAC CGGAAAGTGT GAGTGAAGAA ATCGACATGC ATGTGCAAAG TGATCCAGAA
    AGTCCAACAA ATCTAGGCGT GTACAGGGTT CATGATACAC ATGCTGAAAG CCAACTGGAA
    AGCTTGAGTC CAGAAAGTGA CTCTCATGTG CAAAGTGATC CAGGAAGTCC CACAAAATCA
    GGCCTGTACA GGAGGGGAGC AAAAAATAAG ATTTCCAGTC GTTCAAACAC ACTTGAGGAG
    GACTCAGTGA ATGTGCAGAG GTTAGACACG GTCTGGAATA AGAGTGATCA AGATTCAACC
    AATTTGCTCT TTGATGCTGT GAAGTCTGGC AACTTGGAAA AGTTGGAACA GATGCATGAA
    GAAGGAGCGG ATCTCTCGGT CCAGGACCAT TTAGGTTGCA CCATCCTCCA TCAGGCAGTC
    ATTTTAGGTC ACAAAGAGAT TGTGAAGTTT ATCACTGAAA ACGCTCCTGC CTGCATACTG
    GATGTGACGG AGAAATCAAC AGGAGAGACA GCTCTGCATA AGGCAGCTGC GTTGTGCCAT
    AGAACAATTT GCTGTTATCT GGTGGATGCT GGTGCATCGC TCATGAAGAC AGATCTACAG
    GGTGAAACTC CAAAGATGTA CGCAGAGAGA ACTCGAGACT ACGAGCTAGC TGAATATTTG
    GAAAACCAAC AACACCATCA AATGATCCAG AGAGATGACC ATGAGACGGC AGTGTGA

    The stop codons will be deleted if pcDNA3.1+/C-(K)DYK vector is selected.