cacna2d3 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	cacna2d3
Entrez Gene ID	100148645
Full Name	calcium channel, voltage dependent, alpha2/delta subunit 3
Synonyms	csi:busm1-136d19.1,si:busm1-136d19.1,si:dz136d19.1
General protein information	Preferred Names calcium channel, voltage dependent, alpha2/delta subunit 3 Names calcium channel, voltage dependent, alpha2/delta subunit 3 voltage-dependent calcium channel subunit alpha-2/delta-3
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017358363.1	XP_017213852.1	voltage-dependent calcium channel subunit alpha-2/delta-3 isoform X1
XM_005172923.3	XP_005172980.1	voltage-dependent calcium channel subunit alpha-2/delta-3 isoform X3
XM_017358363.2	XP_017213852.1	voltage-dependent calcium channel subunit alpha-2/delta-3 isoform X1
XM_002663814.5	XP_002663860.2	voltage-dependent calcium channel subunit alpha-2/delta-3 isoform X2
XM_005172924.3	XP_005172981.1	voltage-dependent calcium channel subunit alpha-2/delta-3 isoform X4
XM_005172923.4	XP_005172980.1	voltage-dependent calcium channel subunit alpha-2/delta-3 isoform X3
XM_002663814.6	XP_002663860.2	voltage-dependent calcium channel subunit alpha-2/delta-3 isoform X2
XM_005172924.4	XP_005172981.1	voltage-dependent calcium channel subunit alpha-2/delta-3 isoform X4

Pathways from BioSystems
Gene Ontology

The following cacna2d3 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the cacna2d3 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa54381	XM_017358363.2 Latest version!	Danio rerio calcium channel, voltage dependent, alpha2/delta subunit 3 (cacna2d3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa17276	XM_002663814.6 Latest version!	Danio rerio calcium channel, voltage dependent, alpha2/delta subunit 3 (cacna2d3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa17277	XM_005172923.4 Latest version!	Danio rerio calcium channel, voltage dependent, alpha2/delta subunit 3 (cacna2d3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa17278	XM_005172924.4 Latest version!	Danio rerio calcium channel, voltage dependent, alpha2/delta subunit 3 (cacna2d3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
View Old Accession Versions >>
ODa54381	XM_017358363.1	Danio rerio calcium channel, voltage dependent, alpha2/delta subunit 3 (cacna2d3), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa17277	XM_005172923.3	Danio rerio calcium channel, voltage dependent, alpha2/delta subunit 3 (cacna2d3), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa17276	XM_002663814.5	Danio rerio calcium channel, voltage dependent, alpha2/delta subunit 3 (cacna2d3), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa17278	XM_005172924.3	Danio rerio calcium channel, voltage dependent, alpha2/delta subunit 3 (cacna2d3), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
Hide Old Accession Versions >>

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

cacna2d3 ( XM_017358363.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213852.1 Danio rerio calcium channel, voltage dependent, alpha2/delta subunit 3 (cacna2d3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa54381
Clone ID Related Accession (Same CDS sequence)	XM_017358363.1 , XM_017358363.2
Accession Version	XM_017358363.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent calcium channel subunit alpha-2/delta-3 isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGGA ACCGGAGAAA TCCTGCTCTG CTCTGGTGTG TGATGCTGAG CGCGTGCTGG 
ACGCCGCTGG AGGTGCGGGG GTCCCAACCG GAGATTCCCC TATCAGTGGT GAAACTCTGG 
GCTTCAGCGT TTGGTGGTGA AATAAAATCA ATAGCTTCAA AATACTCAGG ATCTCAGCTC 
TTACAGAAGA AATACAAGGA GTATGAGAAG TCGGTGCGAA TTGAGGAGAT CGACGGCGCA 
AAATTGGTGA AAAATCTGGC GCAGAACATG GAGGAGATGT TTCGGAAAAA AGCGGAGGCC 
ACACGAAGGC TCGTCGAAGC GGCAGAAGAA GCTCATCTTC AGCATGAAGA AAACCCAGAC 
CTACAGTATG AGTATTTCAA CGCAGTGCTG ATAAACGAGG TGGATGAGGA TGGGAATAGC 
GTGGAGCTGG GAGGAGAGTT TCTCCTGGAA CCCAACGATC ATTTTAATAA CCTATCCGTC 
AACCTCAGCC TCAGCGTGGT GCAGGTGCCA ACCAACATGT ACAATAAAGA TCCTGACATA 
GTCAATGGAG TTTACTGGTC GGAAGCCTTG AACAAAGTTT TCGTGGATAA CTTCAGGAGA 
GACCCAACCC TCATCTGGCA GTACTTTGGA AGCGCTAAAG GTTTTTTCCG ACAATACCCA 
GGCGTGAAAT GGTATCCTGA CGAACACGGC GTCATTGCAT TTGATTGCAG AAACAGAAAA 
TGGTATATCC AAGCAGCCAC TTCACCTAAA GATGTTGTGA TTCTAGTGGA CGTCAGTGGC 
AGTATGAAGG GTTTAAGACT GACTATAGCC AGACAGACGG TTGCTTCAAT ACTGGACACA 
CTCGGCGATG ATGACTTCTT CAACATTATT GCATACAACC AAGAGATCCA TTACGTGGAG 
CCCTGTCTAA ACGGCACTCT GGTTCAAGCT GACAGTACTA ATAAGGATCA CTTTAAAGAG 
CATCTGGACA AGTTGTTCGC CAAAGGAATC GGACTCCTGG GCAATGCACT GAGCGAGGCT 
TTCACTATCT TAAATGAGAT TAATCAGACG GGCCGTGGAA GTTCATGTAG TCAGGCGATC 
ATGCTGATAA CAGATGGAGC CACTGAAATG TACGATGATG TTTTCGCCAA ATATAACTGG 
CCTGAACGTA AGGTCCGTAT ATTCCCATAC CTGATTGGCC GTGAATCTGC ATTTGCTGAT 
AACCTAAAAT GGATGGCGTG TGCCAATAAA GGTTACTTTA GTCAGATCTC CACACTGGCT 
GACGTTCAGG AGAATGTGAT GCGGTATTTA CATGTGATGA GCAGACCTAA AGTTATCGAC 
CACGAACATG ACACAGTGTG GACTGAGGCC TACGTCGACA GCGCACTCGC ACAGGCGCAC 
AAGCAGCTTG GAGAGAATAA AGGTCCAAAC CCGATGACCA CAGTGGCCAT GCCTGTGTTT 
AGCACCAAGA ATGAAACGAA AAACCAAGGC ATCCTGCTGG GCGTCGCGGG CACTGATATC 
CCCATTCAGG AGCTAATGAA AGTCATCCCA AAACACAAGC TAGGAATACA CGGATATGCT 
TTTGCCATCA CCAGTAATGG ATATCTCCTG TTGCACCCTG ACCTGAGGCC CCTGTACCAG 
GAGGGCCAGA AGAGGAAGAA ACCCAGTTAC AGCAGTGTGG ACCTGTCCGA GGTGGAGTGG 
GAGGACACAG AGGATGTTTT GCGTACAGCG ATGGTGAACA GGAAAACTGG CACTTTCTCA 
ATGGAAGTAA AAAGAACAGT GGACAAAGGG AAACGAGTCT TGACGATGCA CAATGATTAT 
TACTACACAG ATATTAAAGG AACTCCATTT AGTGTAGGAG TGGCACTCTC TCGAGGACAT 
GGAAAATACT TCTTTAAAGG GAATGTTTCA CTAGAAGCTG GTCTACATGA TCTGGAGCAG 
CCGGATGTAG CCCTTGCTGA TGAATGGACG TACTGTACCA CAGAAGAGCA TCATGAGCAT 
CGACACCTGA CTCAAGTTCA GGCCATCAAA ATCTACATGA CCAGCCGGAA ACCACATTTC 
AAGTGTGACA GAGAGCTGAT TCAGCAGGTT CTGTTTGATG CTGTGGTCAC CGCTCCTGTA 
GAAGCCTACT GGACTGGACT GGCCCTCAAT AAATCTGAGA ACTCAGATAA AGGGGTTGAG 
ATTGCTTTTC TCGGCACCCG GACTGGCCTT TCACGTACCA ACTTGTTTGT AGTTCCTGAG 
CAACTGACCA ATCGAGATTT CCTCACTGCT GAAGATAAGG AGGGCGTCTT TAACGCTGAT 
CACTTTCCTC TGTGGTATAA AAGAGCAGCA GAGCAGGTGC CCGGCACATT TGTGTACTCC 
ATTCCCTTCA GTTCAGGTGG TGAAAATAAG AGTGTGGTTT TGGCAAGCAC TGCTATCCAG 
CTTCTCGATG ACAGAAAGTC TCCTATTGTG GCAGCTGTGG GAATTCAAAT GAAGCTGGAA 
TTCTTTCAGA GAAAATTCTG GACCGCTAGT CGGCAGTGTA ATGCTCTGGA TGGGAAATGC 
ACCATCAGTT GTGATAATGA GGACATTAAA TGCTATCTCA TAGACAACAA TGGCTTCATC 
CTGGTCTCTG AAGACACTTC TCAGACAAAC CTCTTCTTTG GAAAAGTGGA AGGAGCCGTC 
ATGAATAAAC TGCTTCTGAT GGGCTCGTTT AAAAAGATTA ATTTGTATGA CTATCAGGCT 
CTGTGTAAGG AGTACGCAGG CAGCAGTGAC AGCGCACGCA CTTTACTGGA TCCCTTCACC 
ACTGTGAAAT GGCTCTTGAC AGAACTTGTC ATATTTCTGC TGGAGTTTAA CCTGTATAGC 
TGGTGGAACT GTGACTCCAC AGTCAAAGCT CAGAGATCTC AGAGGACAAT GATGGTGCCC 
TGTGACACAG AATACCCAGC CTTCGTCTCA GAGCGTACCA TCAAAGAGAC AACTGGCAAC 
ATTGACTGCA ATGGCTGTAT ACGGACATTT GTGATTCAGC AGATTCCCAG CAGTAACCTG 
TTTATGGTGG TGGTTGAGAA TAAATGTGAC TGTGGCTCTG CTCTTCCAGT TACCATGGAG 
CCCATTGAGA TCATTTATAA TGAGTCACTG AAGTGTGACA GATTGAAGTT TCAGAAGGAT 
CGCAGACGAC CACAGTCCTG CCATCCATTT CATCCAGAGG AGAATGCTGT GGAATGTGGA 
TCAGCGAATG CTTTGAGTTT CCCACTGGTG GCCATTTTAA TCCCAGCATT GTCATTAATG 
ATCAGCAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213852.1
CDS	447..3698
Translation	MQRNRRNPALLWCVMLSACWTPLEVRGSQPEIPLSVVKLWASAFGGEIKSIASKYSGSQLLQKKYKEYEKSVRIEEIDGAKLVKNLAQNMEEMFRKKAEATRRLVEAAEEAHLQHEENPDLQYEYFNAVLINEVDEDGNSVELGGEFLLEPNDHFNNLSVNLSLSVVQVPTNMYNKDPDIVNGVYWSEALNKVFVDNFRRDPTLIWQYFGSAKGFFRQYPGVKWYPDEHGVIAFDCRNRKWYIQAATSPKDVVILVDVSGSMKGLRLTIARQTVASILDTLGDDDFFNIIAYNQEIHYVEPCLNGTLVQADSTNKDHFKEHLDKLFAKGIGLLGNALSEAFTILNEINQTGRGSSCSQAIMLITDGATEMYDDVFAKYNWPERKVRIFPYLIGRESAFADNLKWMACANKGYFSQISTLADVQENVMRYLHVMSRPKVIDHEHDTVWTEAYVDSALAQAHKQLGENKGPNPMTTVAMPVFSTKNETKNQGILLGVAGTDIPIQELMKVIPKHKLGIHGYAFAITSNGYLLLHPDLRPLYQEGQKRKKPSYSSVDLSEVEWEDTEDVLRTAMVNRKTGTFSMEVKRTVDKGKRVLTMHNDYYYTDIKGTPFSVGVALSRGHGKYFFKGNVSLEAGLHDLEQPDVALADEWTYCTTEEHHEHRHLTQVQAIKIYMTSRKPHFKCDRELIQQVLFDAVVTAPVEAYWTGLALNKSENSDKGVEIAFLGTRTGLSRTNLFVVPEQLTNRDFLTAEDKEGVFNADHFPLWYKRAAEQVPGTFVYSIPFSSGGENKSVVLASTAIQLLDDRKSPIVAAVGIQMKLEFFQRKFWTASRQCNALDGKCTISCDNEDIKCYLIDNNGFILVSEDTSQTNLFFGKVEGAVMNKLLLMGSFKKINLYDYQALCKEYAGSSDSARTLLDPFTTVKWLLTELVIFLLEFNLYSWWNCDSTVKAQRSQRTMMVPCDTEYPAFVSERTIKETTGNIDCNGCIRTFVIQQIPSSNLFMVVVENKCDCGSALPVTMEPIEIIYNESLKCDRLKFQKDRRRPQSCHPFHPEENAVECGSANALSFPLVAILIPALSLMISR

Target ORF information:

RefSeq Version	XM_017358363.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage dependent, alpha2/delta subunit 3 (cacna2d3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358363.1

ORF Insert Sequence:

ATGCAGCGGA ACCGGAGAAA TCCTGCTCTG CTCTGGTGTG TGATGCTGAG CGCGTGCTGG 
ACGCCGCTGG AGGTGCGGGG GTCCCAACCG GAGATTCCCC TATCAGTGGT GAAACTCTGG 
GCTTCAGCGT TTGGTGGTGA AATAAAATCA ATAGCTTCAA AATACTCAGG ATCTCAGCTC 
TTACAGAAGA AATACAAGGA GTATGAGAAG TCGGTGCGAA TTGAGGAGAT CGACGGCGCA 
AAATTGGTGA AAAATCTGGC GCAGAACATG GAGGAGATGT TTCGGAAAAA AGCGGAGGCC 
ACACGAAGGC TCGTCGAAGC GGCAGAAGAA GCTCATCTTC AGCATGAAGA AAACCCAGAC 
CTACAGTATG AGTATTTCAA CGCAGTGCTG ATAAACGAGG TGGATGAGGA TGGGAATAGC 
GTGGAGCTGG GAGGAGAGTT TCTCCTGGAA CCCAACGATC ATTTTAATAA CCTATCCGTC 
AACCTCAGCC TCAGCGTGGT GCAGGTGCCA ACCAACATGT ACAATAAAGA TCCTGACATA 
GTCAATGGAG TTTACTGGTC GGAAGCCTTG AACAAAGTTT TCGTGGATAA CTTCAGGAGA 
GACCCAACCC TCATCTGGCA GTACTTTGGA AGCGCTAAAG GTTTTTTCCG ACAATACCCA 
GGCGTGAAAT GGTATCCTGA CGAACACGGC GTCATTGCAT TTGATTGCAG AAACAGAAAA 
TGGTATATCC AAGCAGCCAC TTCACCTAAA GATGTTGTGA TTCTAGTGGA CGTCAGTGGC 
AGTATGAAGG GTTTAAGACT GACTATAGCC AGACAGACGG TTGCTTCAAT ACTGGACACA 
CTCGGCGATG ATGACTTCTT CAACATTATT GCATACAACC AAGAGATCCA TTACGTGGAG 
CCCTGTCTAA ACGGCACTCT GGTTCAAGCT GACAGTACTA ATAAGGATCA CTTTAAAGAG 
CATCTGGACA AGTTGTTCGC CAAAGGAATC GGACTCCTGG GCAATGCACT GAGCGAGGCT 
TTCACTATCT TAAATGAGAT TAATCAGACG GGCCGTGGAA GTTCATGTAG TCAGGCGATC 
ATGCTGATAA CAGATGGAGC CACTGAAATG TACGATGATG TTTTCGCCAA ATATAACTGG 
CCTGAACGTA AGGTCCGTAT ATTCCCATAC CTGATTGGCC GTGAATCTGC ATTTGCTGAT 
AACCTAAAAT GGATGGCGTG TGCCAATAAA GGTTACTTTA GTCAGATCTC CACACTGGCT 
GACGTTCAGG AGAATGTGAT GCGGTATTTA CATGTGATGA GCAGACCTAA AGTTATCGAC 
CACGAACATG ACACAGTGTG GACTGAGGCC TACGTCGACA GCGCACTCGC ACAGGCGCAC 
AAGCAGCTTG GAGAGAATAA AGGTCCAAAC CCGATGACCA CAGTGGCCAT GCCTGTGTTT 
AGCACCAAGA ATGAAACGAA AAACCAAGGC ATCCTGCTGG GCGTCGCGGG CACTGATATC 
CCCATTCAGG AGCTAATGAA AGTCATCCCA AAACACAAGC TAGGAATACA CGGATATGCT 
TTTGCCATCA CCAGTAATGG ATATCTCCTG TTGCACCCTG ACCTGAGGCC CCTGTACCAG 
GAGGGCCAGA AGAGGAAGAA ACCCAGTTAC AGCAGTGTGG ACCTGTCCGA GGTGGAGTGG 
GAGGACACAG AGGATGTTTT GCGTACAGCG ATGGTGAACA GGAAAACTGG CACTTTCTCA 
ATGGAAGTAA AAAGAACAGT GGACAAAGGG AAACGAGTCT TGACGATGCA CAATGATTAT 
TACTACACAG ATATTAAAGG AACTCCATTT AGTGTAGGAG TGGCACTCTC TCGAGGACAT 
GGAAAATACT TCTTTAAAGG GAATGTTTCA CTAGAAGCTG GTCTACATGA TCTGGAGCAG 
CCGGATGTAG CCCTTGCTGA TGAATGGACG TACTGTACCA CAGAAGAGCA TCATGAGCAT 
CGACACCTGA CTCAAGTTCA GGCCATCAAA ATCTACATGA CCAGCCGGAA ACCACATTTC 
AAGTGTGACA GAGAGCTGAT TCAGCAGGTT CTGTTTGATG CTGTGGTCAC CGCTCCTGTA 
GAAGCCTACT GGACTGGACT GGCCCTCAAT AAATCTGAGA ACTCAGATAA AGGGGTTGAG 
ATTGCTTTTC TCGGCACCCG GACTGGCCTT TCACGTACCA ACTTGTTTGT AGTTCCTGAG 
CAACTGACCA ATCGAGATTT CCTCACTGCT GAAGATAAGG AGGGCGTCTT TAACGCTGAT 
CACTTTCCTC TGTGGTATAA AAGAGCAGCA GAGCAGGTGC CCGGCACATT TGTGTACTCC 
ATTCCCTTCA GTTCAGGTGG TGAAAATAAG AGTGTGGTTT TGGCAAGCAC TGCTATCCAG 
CTTCTCGATG ACAGAAAGTC TCCTATTGTG GCAGCTGTGG GAATTCAAAT GAAGCTGGAA 
TTCTTTCAGA GAAAATTCTG GACCGCTAGT CGGCAGTGTA ATGCTCTGGA TGGGAAATGC 
ACCATCAGTT GTGATAATGA GGACATTAAA TGCTATCTCA TAGACAACAA TGGCTTCATC 
CTGGTCTCTG AAGACACTTC TCAGACAAAC CTCTTCTTTG GAAAAGTGGA AGGAGCCGTC 
ATGAATAAAC TGCTTCTGAT GGGCTCGTTT AAAAAGATTA ATTTGTATGA CTATCAGGCT 
CTGTGTAAGG AGTACGCAGG CAGCAGTGAC AGCGCACGCA CTTTACTGGA TCCCTTCACC 
ACTGTGAAAT GGCTCTTGAC AGAACTTGTC ATATTTCTGC TGGAGTTTAA CCTGTATAGC 
TGGTGGAACT GTGACTCCAC AGTCAAAGCT CAGAGATCTC AGAGGACAAT GATGGTGCCC 
TGTGACACAG AATACCCAGC CTTCGTCTCA GAGCGTACCA TCAAAGAGAC AACTGGCAAC 
ATTGACTGCA ATGGCTGTAT ACGGACATTT GTGATTCAGC AGATTCCCAG CAGTAACCTG 
TTTATGGTGG TGGTTGAGAA TAAATGTGAC TGTGGCTCTG CTCTTCCAGT TACCATGGAG 
CCCATTGAGA TCATTTATAA TGAGTCACTG AAGTGTGACA GATTGAAGTT TCAGAAGGAT 
CGCAGACGAC CACAGTCCTG CCATCCATTT CATCCAGAGG AGAATGCTGT GGAATGTGGA 
TCAGCGAATG CTTTGAGTTT CCCACTGGTG GCCATTTTAA TCCCAGCATT GTCATTAATG 
ATCAGCAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna2d3 ( XM_017358363.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_017213852.1 Danio rerio calcium channel, voltage dependent, alpha2/delta subunit 3 (cacna2d3), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa54381
Clone ID Related Accession (Same CDS sequence)	XM_017358363.1 , XM_017358363.2
Accession Version	XM_017358363.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3252bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent calcium channel subunit alpha-2/delta-3 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017358363.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGGA ACCGGAGAAA TCCTGCTCTG CTCTGGTGTG TGATGCTGAG CGCGTGCTGG 
ACGCCGCTGG AGGTGCGGGG GTCCCAACCG GAGATTCCCC TATCAGTGGT GAAACTCTGG 
GCTTCAGCGT TTGGTGGTGA AATAAAATCA ATAGCTTCAA AATACTCAGG ATCTCAGCTC 
TTACAGAAGA AATACAAGGA GTATGAGAAG TCGGTGCGAA TTGAGGAGAT CGACGGCGCA 
AAATTGGTGA AAAATCTGGC GCAGAACATG GAGGAGATGT TTCGGAAAAA AGCGGAGGCC 
ACACGAAGGC TCGTCGAAGC GGCAGAAGAA GCTCATCTTC AGCATGAAGA AAACCCAGAC 
CTACAGTATG AGTATTTCAA CGCAGTGCTG ATAAACGAGG TGGATGAGGA TGGGAATAGC 
GTGGAGCTGG GAGGAGAGTT TCTCCTGGAA CCCAACGATC ATTTTAATAA CCTATCCGTC 
AACCTCAGCC TCAGCGTGGT GCAGGTGCCA ACCAACATGT ACAATAAAGA TCCTGACATA 
GTCAATGGAG TTTACTGGTC GGAAGCCTTG AACAAAGTTT TCGTGGATAA CTTCAGGAGA 
GACCCAACCC TCATCTGGCA GTACTTTGGA AGCGCTAAAG GTTTTTTCCG ACAATACCCA 
GGCGTGAAAT GGTATCCTGA CGAACACGGC GTCATTGCAT TTGATTGCAG AAACAGAAAA 
TGGTATATCC AAGCAGCCAC TTCACCTAAA GATGTTGTGA TTCTAGTGGA CGTCAGTGGC 
AGTATGAAGG GTTTAAGACT GACTATAGCC AGACAGACGG TTGCTTCAAT ACTGGACACA 
CTCGGCGATG ATGACTTCTT CAACATTATT GCATACAACC AAGAGATCCA TTACGTGGAG 
CCCTGTCTAA ACGGCACTCT GGTTCAAGCT GACAGTACTA ATAAGGATCA CTTTAAAGAG 
CATCTGGACA AGTTGTTCGC CAAAGGAATC GGACTCCTGG GCAATGCACT GAGCGAGGCT 
TTCACTATCT TAAATGAGAT TAATCAGACG GGCCGTGGAA GTTCATGTAG TCAGGCGATC 
ATGCTGATAA CAGATGGAGC CACTGAAATG TACGATGATG TTTTCGCCAA ATATAACTGG 
CCTGAACGTA AGGTCCGTAT ATTCCCATAC CTGATTGGCC GTGAATCTGC ATTTGCTGAT 
AACCTAAAAT GGATGGCGTG TGCCAATAAA GGTTACTTTA GTCAGATCTC CACACTGGCT 
GACGTTCAGG AGAATGTGAT GCGGTATTTA CATGTGATGA GCAGACCTAA AGTTATCGAC 
CACGAACATG ACACAGTGTG GACTGAGGCC TACGTCGACA GCGCACTCGC ACAGGCGCAC 
AAGCAGCTTG GAGAGAATAA AGGTCCAAAC CCGATGACCA CAGTGGCCAT GCCTGTGTTT 
AGCACCAAGA ATGAAACGAA AAACCAAGGC ATCCTGCTGG GCGTCGCGGG CACTGATATC 
CCCATTCAGG AGCTAATGAA AGTCATCCCA AAACACAAGC TAGGAATACA CGGATATGCT 
TTTGCCATCA CCAGTAATGG ATATCTCCTG TTGCACCCTG ACCTGAGGCC CCTGTACCAG 
GAGGGCCAGA AGAGGAAGAA ACCCAGTTAC AGCAGTGTGG ACCTGTCCGA GGTGGAGTGG 
GAGGACACAG AGGATGTTTT GCGTACAGCG ATGGTGAACA GGAAAACTGG CACTTTCTCA 
ATGGAAGTAA AAAGAACAGT GGACAAAGGG AAACGAGTCT TGACGATGCA CAATGATTAT 
TACTACACAG ATATTAAAGG AACTCCATTT AGTGTAGGAG TGGCACTCTC TCGAGGACAT 
GGAAAATACT TCTTTAAAGG GAATGTTTCA CTAGAAGCTG GTCTACATGA TCTGGAGCAG 
CCGGATGTAG CCCTTGCTGA TGAATGGACG TACTGTACCA CAGAAGAGCA TCATGAGCAT 
CGACACCTGA CTCAAGTTCA GGCCATCAAA ATCTACATGA CCAGCCGGAA ACCACATTTC 
AAGTGTGACA GAGAGCTGAT TCAGCAGGTT CTGTTTGATG CTGTGGTCAC CGCTCCTGTA 
GAAGCCTACT GGACTGGACT GGCCCTCAAT AAATCTGAGA ACTCAGATAA AGGGGTTGAG 
ATTGCTTTTC TCGGCACCCG GACTGGCCTT TCACGTACCA ACTTGTTTGT AGTTCCTGAG 
CAACTGACCA ATCGAGATTT CCTCACTGCT GAAGATAAGG AGGGCGTCTT TAACGCTGAT 
CACTTTCCTC TGTGGTATAA AAGAGCAGCA GAGCAGGTGC CCGGCACATT TGTGTACTCC 
ATTCCCTTCA GTTCAGGTGG TGAAAATAAG AGTGTGGTTT TGGCAAGCAC TGCTATCCAG 
CTTCTCGATG ACAGAAAGTC TCCTATTGTG GCAGCTGTGG GAATTCAAAT GAAGCTGGAA 
TTCTTTCAGA GAAAATTCTG GACCGCTAGT CGGCAGTGTA ATGCTCTGGA TGGGAAATGC 
ACCATCAGTT GTGATAATGA GGACATTAAA TGCTATCTCA TAGACAACAA TGGCTTCATC 
CTGGTCTCTG AAGACACTTC TCAGACAAAC CTCTTCTTTG GAAAAGTGGA AGGAGCCGTC 
ATGAATAAAC TGCTTCTGAT GGGCTCGTTT AAAAAGATTA ATTTGTATGA CTATCAGGCT 
CTGTGTAAGG AGTACGCAGG CAGCAGTGAC AGCGCACGCA CTTTACTGGA TCCCTTCACC 
ACTGTGAAAT GGCTCTTGAC AGAACTTGTC ATATTTCTGC TGGAGTTTAA CCTGTATAGC 
TGGTGGAACT GTGACTCCAC AGTCAAAGCT CAGAGATCTC AGAGGACAAT GATGGTGCCC 
TGTGACACAG AATACCCAGC CTTCGTCTCA GAGCGTACCA TCAAAGAGAC AACTGGCAAC 
ATTGACTGCA ATGGCTGTAT ACGGACATTT GTGATTCAGC AGATTCCCAG CAGTAACCTG 
TTTATGGTGG TGGTTGAGAA TAAATGTGAC TGTGGCTCTG CTCTTCCAGT TACCATGGAG 
CCCATTGAGA TCATTTATAA TGAGTCACTG AAGTGTGACA GATTGAAGTT TCAGAAGGAT 
CGCAGACGAC CACAGTCCTG CCATCCATTT CATCCAGAGG AGAATGCTGT GGAATGTGGA 
TCAGCGAATG CTTTGAGTTT CCCACTGGTG GCCATTTTAA TCCCAGCATT GTCATTAATG 
ATCAGCAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017213852.1
CDS	544..3795
Translation	MQRNRRNPALLWCVMLSACWTPLEVRGSQPEIPLSVVKLWASAFGGEIKSIASKYSGSQLLQKKYKEYEKSVRIEEIDGAKLVKNLAQNMEEMFRKKAEATRRLVEAAEEAHLQHEENPDLQYEYFNAVLINEVDEDGNSVELGGEFLLEPNDHFNNLSVNLSLSVVQVPTNMYNKDPDIVNGVYWSEALNKVFVDNFRRDPTLIWQYFGSAKGFFRQYPGVKWYPDEHGVIAFDCRNRKWYIQAATSPKDVVILVDVSGSMKGLRLTIARQTVASILDTLGDDDFFNIIAYNQEIHYVEPCLNGTLVQADSTNKDHFKEHLDKLFAKGIGLLGNALSEAFTILNEINQTGRGSSCSQAIMLITDGATEMYDDVFAKYNWPERKVRIFPYLIGRESAFADNLKWMACANKGYFSQISTLADVQENVMRYLHVMSRPKVIDHEHDTVWTEAYVDSALAQAHKQLGENKGPNPMTTVAMPVFSTKNETKNQGILLGVAGTDIPIQELMKVIPKHKLGIHGYAFAITSNGYLLLHPDLRPLYQEGQKRKKPSYSSVDLSEVEWEDTEDVLRTAMVNRKTGTFSMEVKRTVDKGKRVLTMHNDYYYTDIKGTPFSVGVALSRGHGKYFFKGNVSLEAGLHDLEQPDVALADEWTYCTTEEHHEHRHLTQVQAIKIYMTSRKPHFKCDRELIQQVLFDAVVTAPVEAYWTGLALNKSENSDKGVEIAFLGTRTGLSRTNLFVVPEQLTNRDFLTAEDKEGVFNADHFPLWYKRAAEQVPGTFVYSIPFSSGGENKSVVLASTAIQLLDDRKSPIVAAVGIQMKLEFFQRKFWTASRQCNALDGKCTISCDNEDIKCYLIDNNGFILVSEDTSQTNLFFGKVEGAVMNKLLLMGSFKKINLYDYQALCKEYAGSSDSARTLLDPFTTVKWLLTELVIFLLEFNLYSWWNCDSTVKAQRSQRTMMVPCDTEYPAFVSERTIKETTGNIDCNGCIRTFVIQQIPSSNLFMVVVENKCDCGSALPVTMEPIEIIYNESLKCDRLKFQKDRRRPQSCHPFHPEENAVECGSANALSFPLVAILIPALSLMISR

Target ORF information:

RefSeq Version	XM_017358363.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage dependent, alpha2/delta subunit 3 (cacna2d3), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017358363.2

ORF Insert Sequence:

ATGCAGCGGA ACCGGAGAAA TCCTGCTCTG CTCTGGTGTG TGATGCTGAG CGCGTGCTGG 
ACGCCGCTGG AGGTGCGGGG GTCCCAACCG GAGATTCCCC TATCAGTGGT GAAACTCTGG 
GCTTCAGCGT TTGGTGGTGA AATAAAATCA ATAGCTTCAA AATACTCAGG ATCTCAGCTC 
TTACAGAAGA AATACAAGGA GTATGAGAAG TCGGTGCGAA TTGAGGAGAT CGACGGCGCA 
AAATTGGTGA AAAATCTGGC GCAGAACATG GAGGAGATGT TTCGGAAAAA AGCGGAGGCC 
ACACGAAGGC TCGTCGAAGC GGCAGAAGAA GCTCATCTTC AGCATGAAGA AAACCCAGAC 
CTACAGTATG AGTATTTCAA CGCAGTGCTG ATAAACGAGG TGGATGAGGA TGGGAATAGC 
GTGGAGCTGG GAGGAGAGTT TCTCCTGGAA CCCAACGATC ATTTTAATAA CCTATCCGTC 
AACCTCAGCC TCAGCGTGGT GCAGGTGCCA ACCAACATGT ACAATAAAGA TCCTGACATA 
GTCAATGGAG TTTACTGGTC GGAAGCCTTG AACAAAGTTT TCGTGGATAA CTTCAGGAGA 
GACCCAACCC TCATCTGGCA GTACTTTGGA AGCGCTAAAG GTTTTTTCCG ACAATACCCA 
GGCGTGAAAT GGTATCCTGA CGAACACGGC GTCATTGCAT TTGATTGCAG AAACAGAAAA 
TGGTATATCC AAGCAGCCAC TTCACCTAAA GATGTTGTGA TTCTAGTGGA CGTCAGTGGC 
AGTATGAAGG GTTTAAGACT GACTATAGCC AGACAGACGG TTGCTTCAAT ACTGGACACA 
CTCGGCGATG ATGACTTCTT CAACATTATT GCATACAACC AAGAGATCCA TTACGTGGAG 
CCCTGTCTAA ACGGCACTCT GGTTCAAGCT GACAGTACTA ATAAGGATCA CTTTAAAGAG 
CATCTGGACA AGTTGTTCGC CAAAGGAATC GGACTCCTGG GCAATGCACT GAGCGAGGCT 
TTCACTATCT TAAATGAGAT TAATCAGACG GGCCGTGGAA GTTCATGTAG TCAGGCGATC 
ATGCTGATAA CAGATGGAGC CACTGAAATG TACGATGATG TTTTCGCCAA ATATAACTGG 
CCTGAACGTA AGGTCCGTAT ATTCCCATAC CTGATTGGCC GTGAATCTGC ATTTGCTGAT 
AACCTAAAAT GGATGGCGTG TGCCAATAAA GGTTACTTTA GTCAGATCTC CACACTGGCT 
GACGTTCAGG AGAATGTGAT GCGGTATTTA CATGTGATGA GCAGACCTAA AGTTATCGAC 
CACGAACATG ACACAGTGTG GACTGAGGCC TACGTCGACA GCGCACTCGC ACAGGCGCAC 
AAGCAGCTTG GAGAGAATAA AGGTCCAAAC CCGATGACCA CAGTGGCCAT GCCTGTGTTT 
AGCACCAAGA ATGAAACGAA AAACCAAGGC ATCCTGCTGG GCGTCGCGGG CACTGATATC 
CCCATTCAGG AGCTAATGAA AGTCATCCCA AAACACAAGC TAGGAATACA CGGATATGCT 
TTTGCCATCA CCAGTAATGG ATATCTCCTG TTGCACCCTG ACCTGAGGCC CCTGTACCAG 
GAGGGCCAGA AGAGGAAGAA ACCCAGTTAC AGCAGTGTGG ACCTGTCCGA GGTGGAGTGG 
GAGGACACAG AGGATGTTTT GCGTACAGCG ATGGTGAACA GGAAAACTGG CACTTTCTCA 
ATGGAAGTAA AAAGAACAGT GGACAAAGGG AAACGAGTCT TGACGATGCA CAATGATTAT 
TACTACACAG ATATTAAAGG AACTCCATTT AGTGTAGGAG TGGCACTCTC TCGAGGACAT 
GGAAAATACT TCTTTAAAGG GAATGTTTCA CTAGAAGCTG GTCTACATGA TCTGGAGCAG 
CCGGATGTAG CCCTTGCTGA TGAATGGACG TACTGTACCA CAGAAGAGCA TCATGAGCAT 
CGACACCTGA CTCAAGTTCA GGCCATCAAA ATCTACATGA CCAGCCGGAA ACCACATTTC 
AAGTGTGACA GAGAGCTGAT TCAGCAGGTT CTGTTTGATG CTGTGGTCAC CGCTCCTGTA 
GAAGCCTACT GGACTGGACT GGCCCTCAAT AAATCTGAGA ACTCAGATAA AGGGGTTGAG 
ATTGCTTTTC TCGGCACCCG GACTGGCCTT TCACGTACCA ACTTGTTTGT AGTTCCTGAG 
CAACTGACCA ATCGAGATTT CCTCACTGCT GAAGATAAGG AGGGCGTCTT TAACGCTGAT 
CACTTTCCTC TGTGGTATAA AAGAGCAGCA GAGCAGGTGC CCGGCACATT TGTGTACTCC 
ATTCCCTTCA GTTCAGGTGG TGAAAATAAG AGTGTGGTTT TGGCAAGCAC TGCTATCCAG 
CTTCTCGATG ACAGAAAGTC TCCTATTGTG GCAGCTGTGG GAATTCAAAT GAAGCTGGAA 
TTCTTTCAGA GAAAATTCTG GACCGCTAGT CGGCAGTGTA ATGCTCTGGA TGGGAAATGC 
ACCATCAGTT GTGATAATGA GGACATTAAA TGCTATCTCA TAGACAACAA TGGCTTCATC 
CTGGTCTCTG AAGACACTTC TCAGACAAAC CTCTTCTTTG GAAAAGTGGA AGGAGCCGTC 
ATGAATAAAC TGCTTCTGAT GGGCTCGTTT AAAAAGATTA ATTTGTATGA CTATCAGGCT 
CTGTGTAAGG AGTACGCAGG CAGCAGTGAC AGCGCACGCA CTTTACTGGA TCCCTTCACC 
ACTGTGAAAT GGCTCTTGAC AGAACTTGTC ATATTTCTGC TGGAGTTTAA CCTGTATAGC 
TGGTGGAACT GTGACTCCAC AGTCAAAGCT CAGAGATCTC AGAGGACAAT GATGGTGCCC 
TGTGACACAG AATACCCAGC CTTCGTCTCA GAGCGTACCA TCAAAGAGAC AACTGGCAAC 
ATTGACTGCA ATGGCTGTAT ACGGACATTT GTGATTCAGC AGATTCCCAG CAGTAACCTG 
TTTATGGTGG TGGTTGAGAA TAAATGTGAC TGTGGCTCTG CTCTTCCAGT TACCATGGAG 
CCCATTGAGA TCATTTATAA TGAGTCACTG AAGTGTGACA GATTGAAGTT TCAGAAGGAT 
CGCAGACGAC CACAGTCCTG CCATCCATTT CATCCAGAGG AGAATGCTGT GGAATGTGGA 
TCAGCGAATG CTTTGAGTTT CCCACTGGTG GCCATTTTAA TCCCAGCATT GTCATTAATG 
ATCAGCAGGT GA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna2d3 ( XM_002663814.5 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_002663860.2 Danio rerio calcium channel, voltage dependent, alpha2/delta subunit 3 (cacna2d3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa17276
Clone ID Related Accession (Same CDS sequence)	XM_002663814.5 , XM_002663814.6
Accession Version	XM_002663814.5	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent calcium channel subunit alpha-2/delta-3 isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_002663814.4. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGGA ACCGGAGAAA TCCTGCTCTG CTCTGGTGTG TGATGCTGAG CGCGTGCTGG 
ACGCCGCTGG AGGTGCGGGG GTCCCAACCG GAGATTCCCC TATCAGTGGT GAAACTCTGG 
GCTTCAGCGT TTGGTGGTGA AATAAAATCA ATAGCTTCAA AATACTCAGG ATCTCAGCTC 
TTACAGAAGA AATACAAGGA GTATGAGAAG TCGGTGCGAA TTGAGGAGAT CGACGGCGCA 
AAATTGGTGA AAAATCTGGC GCAGAACATG GAGGAGATGT TTCGGAAAAA AGCGGAGGCC 
ACACGAAGGC TCGTCGAAGC GGCAGAAGAA GCTCATCTTC AGCATGAAGA AAACCCAGAC 
CTACAGTATG AGTATTTCAA CGCAGTGCTG ATAAACGAGG TGGATGAGGA TGGGAATAGC 
GTGGAGCTGG GAGGAGAGTT TCTCCTGGAA CCCAACGATC ATTTTAATAA CCTATCCGTC 
AACCTCAGCC TCAGCGTGGT GCAGGTGCCA ACCAACATGT ACAATAAAGA TCCTGACATA 
GTCAATGGAG TTTACTGGTC GGAAGCCTTG AACAAAGTTT TCGTGGATAA CTTCAGGAGA 
GACCCAACCC TCATCTGGCA GTACTTTGGA AGCGCTAAAG GTTTTTTCCG ACAATACCCA 
GGCGTGAAAT GGTATCCTGA CGAACACGGC GTCATTGCAT TTGATTGCAG AAACAGAAAA 
TGGTATATCC AAGCAGCCAC TTCACCTAAA GATGTTGTGA TTCTAGTGGA CGTCAGTGGC 
AGTATGAAGG GTTTAAGACT GACTATAGCC AGACAGACGG TTGCTTCAAT ACTGGACACA 
CTCGGCGATG ATGACTTCTT CAACATTATT GCATACAACC AAGAGATCCA TTACGTGGAG 
CCCTGTCTAA ACGGCACTCT GGTTCAAGCT GACAGTACTA ATAAGGATCA CTTTAAAGAG 
CATCTGGACA AGTTGTTCGC CAAAGGAATC GGACTCCTGG GCAATGCACT GAGCGAGGCT 
TTCACTATCT TAAATGAGAT TAATCAGACG GGCCGTGGAA GTTCATGTAG TCAGGCGATC 
ATGCTGATAA CAGATGGAGC CACTGAAATG TACGATGATG TTTTCGCCAA ATATAACTGG 
CCTGAACGTA AGGTCCGTAT ATTCCCATAC CTGATTGGCC GTGAATCTGC ATTTGCTGAT 
AACCTAAAAT GGATGGCGTG TGCCAATAAA GGTTACTTTA GTCAGATCTC CACACTGGCT 
GACGTTCAGG AGAATGTGAT GCGGTATTTA CATGTGATGA GCAGACCTAA AGTTATCGAC 
CACGAACATG ACACAGTGTG GACTGAGGCC TACGTCGACA GCGCACTCGC ACAGGCGCAC 
AAGCTTGGAG AGAATAAAGG TCCAAACCCG ATGACCACAG TGGCCATGCC TGTGTTTAGC 
ACCAAGAATG AAACGAAAAA CCAAGGCATC CTGCTGGGCG TCGCGGGCAC TGATATCCCC 
ATTCAGGAGC TAATGAAAGT CATCCCAAAA CACAAGCTAG GAATACACGG ATATGCTTTT 
GCCATCACCA GTAATGGATA TCTCCTGTTG CACCCTGACC TGAGGCCCCT GTACCAGGAG 
GGCCAGAAGA GGAAGAAACC CAGTTACAGC AGTGTGGACC TGTCCGAGGT GGAGTGGGAG 
GACACAGAGG ATGTTTTGCG TACAGCGATG GTGAACAGGA AAACTGGCAC TTTCTCAATG 
GAAGTAAAAA GAACAGTGGA CAAAGGGAAA CGAGTCTTGA CGATGCACAA TGATTATTAC 
TACACAGATA TTAAAGGAAC TCCATTTAGT GTAGGAGTGG CACTCTCTCG AGGACATGGA 
AAATACTTCT TTAAAGGGAA TGTTTCACTA GAAGCTGGTC TACATGATCT GGAGCAGCCG 
GATGTAGCCC TTGCTGATGA ATGGACGTAC TGTACCACAG AAGAGCATCA TGAGCATCGA 
CACCTGACTC AAGTTCAGGC CATCAAAATC TACATGACCA GCCGGAAACC ACATTTCAAG 
TGTGACAGAG AGCTGATTCA GCAGGTTCTG TTTGATGCTG TGGTCACCGC TCCTGTAGAA 
GCCTACTGGA CTGGACTGGC CCTCAATAAA TCTGAGAACT CAGATAAAGG GGTTGAGATT 
GCTTTTCTCG GCACCCGGAC TGGCCTTTCA CGTACCAACT TGTTTGTAGT TCCTGAGCAA 
CTGACCAATC GAGATTTCCT CACTGCTGAA GATAAGGAGG GCGTCTTTAA CGCTGATCAC 
TTTCCTCTGT GGTATAAAAG AGCAGCAGAG CAGGTGCCCG GCACATTTGT GTACTCCATT 
CCCTTCAGTT CAGGTGGTGA AAATAAGAGT GTGGTTTTGG CAAGCACTGC TATCCAGCTT 
CTCGATGACA GAAAGTCTCC TATTGTGGCA GCTGTGGGAA TTCAAATGAA GCTGGAATTC 
TTTCAGAGAA AATTCTGGAC CGCTAGTCGG CAGTGTAATG CTCTGGATGG GAAATGCACC 
ATCAGTTGTG ATAATGAGGA CATTAAATGC TATCTCATAG ACAACAATGG CTTCATCCTG 
GTCTCTGAAG ACACTTCTCA GACAAACCTC TTCTTTGGAA AAGTGGAAGG AGCCGTCATG 
AATAAACTGC TTCTGATGGG CTCGTTTAAA AAGATTAATT TGTATGACTA TCAGGCTCTG 
TGTAAGGAGT ACGCAGGCAG CAGTGACAGC GCACGCACTT TACTGGATCC CTTCACCACT 
GTGAAATGGC TCTTGACAGA ACTTGTCATA TTTCTGCTGG AGTTTAACCT GTATAGCTGG 
TGGAACTGTG ACTCCACAGT CAAAGCTCAG AGATCTCAGA GGACAATGAT GGTGCCCTGT 
GACACAGAAT ACCCAGCCTT CGTCTCAGAG CGTACCATCA AAGAGACAAC TGGCAACATT 
GACTGCAATG GCTGTATACG GACATTTGTG ATTCAGCAGA TTCCCAGCAG TAACCTGTTT 
ATGGTGGTGG TTGAGAATAA ATGTGACTGT GGCTCTGCTC TTCCAGTTAC CATGGAGCCC 
ATTGAGATCA TTTATAATGA GTCACTGAAG TGTGACAGAT TGAAGTTTCA GAAGGATCGC 
AGACGACCAC AGTCCTGCCA TCCATTTCAT CCAGAGGAGA ATGCTGTGGA ATGTGGATCA 
GCGAATGCTT TGAGTTTCCC ACTGGTGGCC ATTTTAATCC CAGCATTGTC ATTAATGATC 
AGCAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002663860.2
CDS	447..3695
Translation	MQRNRRNPALLWCVMLSACWTPLEVRGSQPEIPLSVVKLWASAFGGEIKSIASKYSGSQLLQKKYKEYEKSVRIEEIDGAKLVKNLAQNMEEMFRKKAEATRRLVEAAEEAHLQHEENPDLQYEYFNAVLINEVDEDGNSVELGGEFLLEPNDHFNNLSVNLSLSVVQVPTNMYNKDPDIVNGVYWSEALNKVFVDNFRRDPTLIWQYFGSAKGFFRQYPGVKWYPDEHGVIAFDCRNRKWYIQAATSPKDVVILVDVSGSMKGLRLTIARQTVASILDTLGDDDFFNIIAYNQEIHYVEPCLNGTLVQADSTNKDHFKEHLDKLFAKGIGLLGNALSEAFTILNEINQTGRGSSCSQAIMLITDGATEMYDDVFAKYNWPERKVRIFPYLIGRESAFADNLKWMACANKGYFSQISTLADVQENVMRYLHVMSRPKVIDHEHDTVWTEAYVDSALAQAHKLGENKGPNPMTTVAMPVFSTKNETKNQGILLGVAGTDIPIQELMKVIPKHKLGIHGYAFAITSNGYLLLHPDLRPLYQEGQKRKKPSYSSVDLSEVEWEDTEDVLRTAMVNRKTGTFSMEVKRTVDKGKRVLTMHNDYYYTDIKGTPFSVGVALSRGHGKYFFKGNVSLEAGLHDLEQPDVALADEWTYCTTEEHHEHRHLTQVQAIKIYMTSRKPHFKCDRELIQQVLFDAVVTAPVEAYWTGLALNKSENSDKGVEIAFLGTRTGLSRTNLFVVPEQLTNRDFLTAEDKEGVFNADHFPLWYKRAAEQVPGTFVYSIPFSSGGENKSVVLASTAIQLLDDRKSPIVAAVGIQMKLEFFQRKFWTASRQCNALDGKCTISCDNEDIKCYLIDNNGFILVSEDTSQTNLFFGKVEGAVMNKLLLMGSFKKINLYDYQALCKEYAGSSDSARTLLDPFTTVKWLLTELVIFLLEFNLYSWWNCDSTVKAQRSQRTMMVPCDTEYPAFVSERTIKETTGNIDCNGCIRTFVIQQIPSSNLFMVVVENKCDCGSALPVTMEPIEIIYNESLKCDRLKFQKDRRRPQSCHPFHPEENAVECGSANALSFPLVAILIPALSLMISR

Target ORF information:

RefSeq Version	XM_002663814.5
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage dependent, alpha2/delta subunit 3 (cacna2d3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002663814.5

ORF Insert Sequence:

ATGCAGCGGA ACCGGAGAAA TCCTGCTCTG CTCTGGTGTG TGATGCTGAG CGCGTGCTGG 
ACGCCGCTGG AGGTGCGGGG GTCCCAACCG GAGATTCCCC TATCAGTGGT GAAACTCTGG 
GCTTCAGCGT TTGGTGGTGA AATAAAATCA ATAGCTTCAA AATACTCAGG ATCTCAGCTC 
TTACAGAAGA AATACAAGGA GTATGAGAAG TCGGTGCGAA TTGAGGAGAT CGACGGCGCA 
AAATTGGTGA AAAATCTGGC GCAGAACATG GAGGAGATGT TTCGGAAAAA AGCGGAGGCC 
ACACGAAGGC TCGTCGAAGC GGCAGAAGAA GCTCATCTTC AGCATGAAGA AAACCCAGAC 
CTACAGTATG AGTATTTCAA CGCAGTGCTG ATAAACGAGG TGGATGAGGA TGGGAATAGC 
GTGGAGCTGG GAGGAGAGTT TCTCCTGGAA CCCAACGATC ATTTTAATAA CCTATCCGTC 
AACCTCAGCC TCAGCGTGGT GCAGGTGCCA ACCAACATGT ACAATAAAGA TCCTGACATA 
GTCAATGGAG TTTACTGGTC GGAAGCCTTG AACAAAGTTT TCGTGGATAA CTTCAGGAGA 
GACCCAACCC TCATCTGGCA GTACTTTGGA AGCGCTAAAG GTTTTTTCCG ACAATACCCA 
GGCGTGAAAT GGTATCCTGA CGAACACGGC GTCATTGCAT TTGATTGCAG AAACAGAAAA 
TGGTATATCC AAGCAGCCAC TTCACCTAAA GATGTTGTGA TTCTAGTGGA CGTCAGTGGC 
AGTATGAAGG GTTTAAGACT GACTATAGCC AGACAGACGG TTGCTTCAAT ACTGGACACA 
CTCGGCGATG ATGACTTCTT CAACATTATT GCATACAACC AAGAGATCCA TTACGTGGAG 
CCCTGTCTAA ACGGCACTCT GGTTCAAGCT GACAGTACTA ATAAGGATCA CTTTAAAGAG 
CATCTGGACA AGTTGTTCGC CAAAGGAATC GGACTCCTGG GCAATGCACT GAGCGAGGCT 
TTCACTATCT TAAATGAGAT TAATCAGACG GGCCGTGGAA GTTCATGTAG TCAGGCGATC 
ATGCTGATAA CAGATGGAGC CACTGAAATG TACGATGATG TTTTCGCCAA ATATAACTGG 
CCTGAACGTA AGGTCCGTAT ATTCCCATAC CTGATTGGCC GTGAATCTGC ATTTGCTGAT 
AACCTAAAAT GGATGGCGTG TGCCAATAAA GGTTACTTTA GTCAGATCTC CACACTGGCT 
GACGTTCAGG AGAATGTGAT GCGGTATTTA CATGTGATGA GCAGACCTAA AGTTATCGAC 
CACGAACATG ACACAGTGTG GACTGAGGCC TACGTCGACA GCGCACTCGC ACAGGCGCAC 
AAGCTTGGAG AGAATAAAGG TCCAAACCCG ATGACCACAG TGGCCATGCC TGTGTTTAGC 
ACCAAGAATG AAACGAAAAA CCAAGGCATC CTGCTGGGCG TCGCGGGCAC TGATATCCCC 
ATTCAGGAGC TAATGAAAGT CATCCCAAAA CACAAGCTAG GAATACACGG ATATGCTTTT 
GCCATCACCA GTAATGGATA TCTCCTGTTG CACCCTGACC TGAGGCCCCT GTACCAGGAG 
GGCCAGAAGA GGAAGAAACC CAGTTACAGC AGTGTGGACC TGTCCGAGGT GGAGTGGGAG 
GACACAGAGG ATGTTTTGCG TACAGCGATG GTGAACAGGA AAACTGGCAC TTTCTCAATG 
GAAGTAAAAA GAACAGTGGA CAAAGGGAAA CGAGTCTTGA CGATGCACAA TGATTATTAC 
TACACAGATA TTAAAGGAAC TCCATTTAGT GTAGGAGTGG CACTCTCTCG AGGACATGGA 
AAATACTTCT TTAAAGGGAA TGTTTCACTA GAAGCTGGTC TACATGATCT GGAGCAGCCG 
GATGTAGCCC TTGCTGATGA ATGGACGTAC TGTACCACAG AAGAGCATCA TGAGCATCGA 
CACCTGACTC AAGTTCAGGC CATCAAAATC TACATGACCA GCCGGAAACC ACATTTCAAG 
TGTGACAGAG AGCTGATTCA GCAGGTTCTG TTTGATGCTG TGGTCACCGC TCCTGTAGAA 
GCCTACTGGA CTGGACTGGC CCTCAATAAA TCTGAGAACT CAGATAAAGG GGTTGAGATT 
GCTTTTCTCG GCACCCGGAC TGGCCTTTCA CGTACCAACT TGTTTGTAGT TCCTGAGCAA 
CTGACCAATC GAGATTTCCT CACTGCTGAA GATAAGGAGG GCGTCTTTAA CGCTGATCAC 
TTTCCTCTGT GGTATAAAAG AGCAGCAGAG CAGGTGCCCG GCACATTTGT GTACTCCATT 
CCCTTCAGTT CAGGTGGTGA AAATAAGAGT GTGGTTTTGG CAAGCACTGC TATCCAGCTT 
CTCGATGACA GAAAGTCTCC TATTGTGGCA GCTGTGGGAA TTCAAATGAA GCTGGAATTC 
TTTCAGAGAA AATTCTGGAC CGCTAGTCGG CAGTGTAATG CTCTGGATGG GAAATGCACC 
ATCAGTTGTG ATAATGAGGA CATTAAATGC TATCTCATAG ACAACAATGG CTTCATCCTG 
GTCTCTGAAG ACACTTCTCA GACAAACCTC TTCTTTGGAA AAGTGGAAGG AGCCGTCATG 
AATAAACTGC TTCTGATGGG CTCGTTTAAA AAGATTAATT TGTATGACTA TCAGGCTCTG 
TGTAAGGAGT ACGCAGGCAG CAGTGACAGC GCACGCACTT TACTGGATCC CTTCACCACT 
GTGAAATGGC TCTTGACAGA ACTTGTCATA TTTCTGCTGG AGTTTAACCT GTATAGCTGG 
TGGAACTGTG ACTCCACAGT CAAAGCTCAG AGATCTCAGA GGACAATGAT GGTGCCCTGT 
GACACAGAAT ACCCAGCCTT CGTCTCAGAG CGTACCATCA AAGAGACAAC TGGCAACATT 
GACTGCAATG GCTGTATACG GACATTTGTG ATTCAGCAGA TTCCCAGCAG TAACCTGTTT 
ATGGTGGTGG TTGAGAATAA ATGTGACTGT GGCTCTGCTC TTCCAGTTAC CATGGAGCCC 
ATTGAGATCA TTTATAATGA GTCACTGAAG TGTGACAGAT TGAAGTTTCA GAAGGATCGC 
AGACGACCAC AGTCCTGCCA TCCATTTCAT CCAGAGGAGA ATGCTGTGGA ATGTGGATCA 
GCGAATGCTT TGAGTTTCCC ACTGGTGGCC ATTTTAATCC CAGCATTGTC ATTAATGATC 
AGCAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna2d3 ( XM_002663814.6 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_002663860.2 Danio rerio calcium channel, voltage dependent, alpha2/delta subunit 3 (cacna2d3), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa17276
Clone ID Related Accession (Same CDS sequence)	XM_002663814.5 , XM_002663814.6
Accession Version	XM_002663814.6 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent calcium channel subunit alpha-2/delta-3 isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_002663814.5. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGGA ACCGGAGAAA TCCTGCTCTG CTCTGGTGTG TGATGCTGAG CGCGTGCTGG 
ACGCCGCTGG AGGTGCGGGG GTCCCAACCG GAGATTCCCC TATCAGTGGT GAAACTCTGG 
GCTTCAGCGT TTGGTGGTGA AATAAAATCA ATAGCTTCAA AATACTCAGG ATCTCAGCTC 
TTACAGAAGA AATACAAGGA GTATGAGAAG TCGGTGCGAA TTGAGGAGAT CGACGGCGCA 
AAATTGGTGA AAAATCTGGC GCAGAACATG GAGGAGATGT TTCGGAAAAA AGCGGAGGCC 
ACACGAAGGC TCGTCGAAGC GGCAGAAGAA GCTCATCTTC AGCATGAAGA AAACCCAGAC 
CTACAGTATG AGTATTTCAA CGCAGTGCTG ATAAACGAGG TGGATGAGGA TGGGAATAGC 
GTGGAGCTGG GAGGAGAGTT TCTCCTGGAA CCCAACGATC ATTTTAATAA CCTATCCGTC 
AACCTCAGCC TCAGCGTGGT GCAGGTGCCA ACCAACATGT ACAATAAAGA TCCTGACATA 
GTCAATGGAG TTTACTGGTC GGAAGCCTTG AACAAAGTTT TCGTGGATAA CTTCAGGAGA 
GACCCAACCC TCATCTGGCA GTACTTTGGA AGCGCTAAAG GTTTTTTCCG ACAATACCCA 
GGCGTGAAAT GGTATCCTGA CGAACACGGC GTCATTGCAT TTGATTGCAG AAACAGAAAA 
TGGTATATCC AAGCAGCCAC TTCACCTAAA GATGTTGTGA TTCTAGTGGA CGTCAGTGGC 
AGTATGAAGG GTTTAAGACT GACTATAGCC AGACAGACGG TTGCTTCAAT ACTGGACACA 
CTCGGCGATG ATGACTTCTT CAACATTATT GCATACAACC AAGAGATCCA TTACGTGGAG 
CCCTGTCTAA ACGGCACTCT GGTTCAAGCT GACAGTACTA ATAAGGATCA CTTTAAAGAG 
CATCTGGACA AGTTGTTCGC CAAAGGAATC GGACTCCTGG GCAATGCACT GAGCGAGGCT 
TTCACTATCT TAAATGAGAT TAATCAGACG GGCCGTGGAA GTTCATGTAG TCAGGCGATC 
ATGCTGATAA CAGATGGAGC CACTGAAATG TACGATGATG TTTTCGCCAA ATATAACTGG 
CCTGAACGTA AGGTCCGTAT ATTCCCATAC CTGATTGGCC GTGAATCTGC ATTTGCTGAT 
AACCTAAAAT GGATGGCGTG TGCCAATAAA GGTTACTTTA GTCAGATCTC CACACTGGCT 
GACGTTCAGG AGAATGTGAT GCGGTATTTA CATGTGATGA GCAGACCTAA AGTTATCGAC 
CACGAACATG ACACAGTGTG GACTGAGGCC TACGTCGACA GCGCACTCGC ACAGGCGCAC 
AAGCTTGGAG AGAATAAAGG TCCAAACCCG ATGACCACAG TGGCCATGCC TGTGTTTAGC 
ACCAAGAATG AAACGAAAAA CCAAGGCATC CTGCTGGGCG TCGCGGGCAC TGATATCCCC 
ATTCAGGAGC TAATGAAAGT CATCCCAAAA CACAAGCTAG GAATACACGG ATATGCTTTT 
GCCATCACCA GTAATGGATA TCTCCTGTTG CACCCTGACC TGAGGCCCCT GTACCAGGAG 
GGCCAGAAGA GGAAGAAACC CAGTTACAGC AGTGTGGACC TGTCCGAGGT GGAGTGGGAG 
GACACAGAGG ATGTTTTGCG TACAGCGATG GTGAACAGGA AAACTGGCAC TTTCTCAATG 
GAAGTAAAAA GAACAGTGGA CAAAGGGAAA CGAGTCTTGA CGATGCACAA TGATTATTAC 
TACACAGATA TTAAAGGAAC TCCATTTAGT GTAGGAGTGG CACTCTCTCG AGGACATGGA 
AAATACTTCT TTAAAGGGAA TGTTTCACTA GAAGCTGGTC TACATGATCT GGAGCAGCCG 
GATGTAGCCC TTGCTGATGA ATGGACGTAC TGTACCACAG AAGAGCATCA TGAGCATCGA 
CACCTGACTC AAGTTCAGGC CATCAAAATC TACATGACCA GCCGGAAACC ACATTTCAAG 
TGTGACAGAG AGCTGATTCA GCAGGTTCTG TTTGATGCTG TGGTCACCGC TCCTGTAGAA 
GCCTACTGGA CTGGACTGGC CCTCAATAAA TCTGAGAACT CAGATAAAGG GGTTGAGATT 
GCTTTTCTCG GCACCCGGAC TGGCCTTTCA CGTACCAACT TGTTTGTAGT TCCTGAGCAA 
CTGACCAATC GAGATTTCCT CACTGCTGAA GATAAGGAGG GCGTCTTTAA CGCTGATCAC 
TTTCCTCTGT GGTATAAAAG AGCAGCAGAG CAGGTGCCCG GCACATTTGT GTACTCCATT 
CCCTTCAGTT CAGGTGGTGA AAATAAGAGT GTGGTTTTGG CAAGCACTGC TATCCAGCTT 
CTCGATGACA GAAAGTCTCC TATTGTGGCA GCTGTGGGAA TTCAAATGAA GCTGGAATTC 
TTTCAGAGAA AATTCTGGAC CGCTAGTCGG CAGTGTAATG CTCTGGATGG GAAATGCACC 
ATCAGTTGTG ATAATGAGGA CATTAAATGC TATCTCATAG ACAACAATGG CTTCATCCTG 
GTCTCTGAAG ACACTTCTCA GACAAACCTC TTCTTTGGAA AAGTGGAAGG AGCCGTCATG 
AATAAACTGC TTCTGATGGG CTCGTTTAAA AAGATTAATT TGTATGACTA TCAGGCTCTG 
TGTAAGGAGT ACGCAGGCAG CAGTGACAGC GCACGCACTT TACTGGATCC CTTCACCACT 
GTGAAATGGC TCTTGACAGA ACTTGTCATA TTTCTGCTGG AGTTTAACCT GTATAGCTGG 
TGGAACTGTG ACTCCACAGT CAAAGCTCAG AGATCTCAGA GGACAATGAT GGTGCCCTGT 
GACACAGAAT ACCCAGCCTT CGTCTCAGAG CGTACCATCA AAGAGACAAC TGGCAACATT 
GACTGCAATG GCTGTATACG GACATTTGTG ATTCAGCAGA TTCCCAGCAG TAACCTGTTT 
ATGGTGGTGG TTGAGAATAA ATGTGACTGT GGCTCTGCTC TTCCAGTTAC CATGGAGCCC 
ATTGAGATCA TTTATAATGA GTCACTGAAG TGTGACAGAT TGAAGTTTCA GAAGGATCGC 
AGACGACCAC AGTCCTGCCA TCCATTTCAT CCAGAGGAGA ATGCTGTGGA ATGTGGATCA 
GCGAATGCTT TGAGTTTCCC ACTGGTGGCC ATTTTAATCC CAGCATTGTC ATTAATGATC 
AGCAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_002663860.2
CDS	546..3794
Translation	MQRNRRNPALLWCVMLSACWTPLEVRGSQPEIPLSVVKLWASAFGGEIKSIASKYSGSQLLQKKYKEYEKSVRIEEIDGAKLVKNLAQNMEEMFRKKAEATRRLVEAAEEAHLQHEENPDLQYEYFNAVLINEVDEDGNSVELGGEFLLEPNDHFNNLSVNLSLSVVQVPTNMYNKDPDIVNGVYWSEALNKVFVDNFRRDPTLIWQYFGSAKGFFRQYPGVKWYPDEHGVIAFDCRNRKWYIQAATSPKDVVILVDVSGSMKGLRLTIARQTVASILDTLGDDDFFNIIAYNQEIHYVEPCLNGTLVQADSTNKDHFKEHLDKLFAKGIGLLGNALSEAFTILNEINQTGRGSSCSQAIMLITDGATEMYDDVFAKYNWPERKVRIFPYLIGRESAFADNLKWMACANKGYFSQISTLADVQENVMRYLHVMSRPKVIDHEHDTVWTEAYVDSALAQAHKLGENKGPNPMTTVAMPVFSTKNETKNQGILLGVAGTDIPIQELMKVIPKHKLGIHGYAFAITSNGYLLLHPDLRPLYQEGQKRKKPSYSSVDLSEVEWEDTEDVLRTAMVNRKTGTFSMEVKRTVDKGKRVLTMHNDYYYTDIKGTPFSVGVALSRGHGKYFFKGNVSLEAGLHDLEQPDVALADEWTYCTTEEHHEHRHLTQVQAIKIYMTSRKPHFKCDRELIQQVLFDAVVTAPVEAYWTGLALNKSENSDKGVEIAFLGTRTGLSRTNLFVVPEQLTNRDFLTAEDKEGVFNADHFPLWYKRAAEQVPGTFVYSIPFSSGGENKSVVLASTAIQLLDDRKSPIVAAVGIQMKLEFFQRKFWTASRQCNALDGKCTISCDNEDIKCYLIDNNGFILVSEDTSQTNLFFGKVEGAVMNKLLLMGSFKKINLYDYQALCKEYAGSSDSARTLLDPFTTVKWLLTELVIFLLEFNLYSWWNCDSTVKAQRSQRTMMVPCDTEYPAFVSERTIKETTGNIDCNGCIRTFVIQQIPSSNLFMVVVENKCDCGSALPVTMEPIEIIYNESLKCDRLKFQKDRRRPQSCHPFHPEENAVECGSANALSFPLVAILIPALSLMISR

Target ORF information:

RefSeq Version	XM_002663814.6
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage dependent, alpha2/delta subunit 3 (cacna2d3), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_002663814.6

ORF Insert Sequence:

ATGCAGCGGA ACCGGAGAAA TCCTGCTCTG CTCTGGTGTG TGATGCTGAG CGCGTGCTGG 
ACGCCGCTGG AGGTGCGGGG GTCCCAACCG GAGATTCCCC TATCAGTGGT GAAACTCTGG 
GCTTCAGCGT TTGGTGGTGA AATAAAATCA ATAGCTTCAA AATACTCAGG ATCTCAGCTC 
TTACAGAAGA AATACAAGGA GTATGAGAAG TCGGTGCGAA TTGAGGAGAT CGACGGCGCA 
AAATTGGTGA AAAATCTGGC GCAGAACATG GAGGAGATGT TTCGGAAAAA AGCGGAGGCC 
ACACGAAGGC TCGTCGAAGC GGCAGAAGAA GCTCATCTTC AGCATGAAGA AAACCCAGAC 
CTACAGTATG AGTATTTCAA CGCAGTGCTG ATAAACGAGG TGGATGAGGA TGGGAATAGC 
GTGGAGCTGG GAGGAGAGTT TCTCCTGGAA CCCAACGATC ATTTTAATAA CCTATCCGTC 
AACCTCAGCC TCAGCGTGGT GCAGGTGCCA ACCAACATGT ACAATAAAGA TCCTGACATA 
GTCAATGGAG TTTACTGGTC GGAAGCCTTG AACAAAGTTT TCGTGGATAA CTTCAGGAGA 
GACCCAACCC TCATCTGGCA GTACTTTGGA AGCGCTAAAG GTTTTTTCCG ACAATACCCA 
GGCGTGAAAT GGTATCCTGA CGAACACGGC GTCATTGCAT TTGATTGCAG AAACAGAAAA 
TGGTATATCC AAGCAGCCAC TTCACCTAAA GATGTTGTGA TTCTAGTGGA CGTCAGTGGC 
AGTATGAAGG GTTTAAGACT GACTATAGCC AGACAGACGG TTGCTTCAAT ACTGGACACA 
CTCGGCGATG ATGACTTCTT CAACATTATT GCATACAACC AAGAGATCCA TTACGTGGAG 
CCCTGTCTAA ACGGCACTCT GGTTCAAGCT GACAGTACTA ATAAGGATCA CTTTAAAGAG 
CATCTGGACA AGTTGTTCGC CAAAGGAATC GGACTCCTGG GCAATGCACT GAGCGAGGCT 
TTCACTATCT TAAATGAGAT TAATCAGACG GGCCGTGGAA GTTCATGTAG TCAGGCGATC 
ATGCTGATAA CAGATGGAGC CACTGAAATG TACGATGATG TTTTCGCCAA ATATAACTGG 
CCTGAACGTA AGGTCCGTAT ATTCCCATAC CTGATTGGCC GTGAATCTGC ATTTGCTGAT 
AACCTAAAAT GGATGGCGTG TGCCAATAAA GGTTACTTTA GTCAGATCTC CACACTGGCT 
GACGTTCAGG AGAATGTGAT GCGGTATTTA CATGTGATGA GCAGACCTAA AGTTATCGAC 
CACGAACATG ACACAGTGTG GACTGAGGCC TACGTCGACA GCGCACTCGC ACAGGCGCAC 
AAGCTTGGAG AGAATAAAGG TCCAAACCCG ATGACCACAG TGGCCATGCC TGTGTTTAGC 
ACCAAGAATG AAACGAAAAA CCAAGGCATC CTGCTGGGCG TCGCGGGCAC TGATATCCCC 
ATTCAGGAGC TAATGAAAGT CATCCCAAAA CACAAGCTAG GAATACACGG ATATGCTTTT 
GCCATCACCA GTAATGGATA TCTCCTGTTG CACCCTGACC TGAGGCCCCT GTACCAGGAG 
GGCCAGAAGA GGAAGAAACC CAGTTACAGC AGTGTGGACC TGTCCGAGGT GGAGTGGGAG 
GACACAGAGG ATGTTTTGCG TACAGCGATG GTGAACAGGA AAACTGGCAC TTTCTCAATG 
GAAGTAAAAA GAACAGTGGA CAAAGGGAAA CGAGTCTTGA CGATGCACAA TGATTATTAC 
TACACAGATA TTAAAGGAAC TCCATTTAGT GTAGGAGTGG CACTCTCTCG AGGACATGGA 
AAATACTTCT TTAAAGGGAA TGTTTCACTA GAAGCTGGTC TACATGATCT GGAGCAGCCG 
GATGTAGCCC TTGCTGATGA ATGGACGTAC TGTACCACAG AAGAGCATCA TGAGCATCGA 
CACCTGACTC AAGTTCAGGC CATCAAAATC TACATGACCA GCCGGAAACC ACATTTCAAG 
TGTGACAGAG AGCTGATTCA GCAGGTTCTG TTTGATGCTG TGGTCACCGC TCCTGTAGAA 
GCCTACTGGA CTGGACTGGC CCTCAATAAA TCTGAGAACT CAGATAAAGG GGTTGAGATT 
GCTTTTCTCG GCACCCGGAC TGGCCTTTCA CGTACCAACT TGTTTGTAGT TCCTGAGCAA 
CTGACCAATC GAGATTTCCT CACTGCTGAA GATAAGGAGG GCGTCTTTAA CGCTGATCAC 
TTTCCTCTGT GGTATAAAAG AGCAGCAGAG CAGGTGCCCG GCACATTTGT GTACTCCATT 
CCCTTCAGTT CAGGTGGTGA AAATAAGAGT GTGGTTTTGG CAAGCACTGC TATCCAGCTT 
CTCGATGACA GAAAGTCTCC TATTGTGGCA GCTGTGGGAA TTCAAATGAA GCTGGAATTC 
TTTCAGAGAA AATTCTGGAC CGCTAGTCGG CAGTGTAATG CTCTGGATGG GAAATGCACC 
ATCAGTTGTG ATAATGAGGA CATTAAATGC TATCTCATAG ACAACAATGG CTTCATCCTG 
GTCTCTGAAG ACACTTCTCA GACAAACCTC TTCTTTGGAA AAGTGGAAGG AGCCGTCATG 
AATAAACTGC TTCTGATGGG CTCGTTTAAA AAGATTAATT TGTATGACTA TCAGGCTCTG 
TGTAAGGAGT ACGCAGGCAG CAGTGACAGC GCACGCACTT TACTGGATCC CTTCACCACT 
GTGAAATGGC TCTTGACAGA ACTTGTCATA TTTCTGCTGG AGTTTAACCT GTATAGCTGG 
TGGAACTGTG ACTCCACAGT CAAAGCTCAG AGATCTCAGA GGACAATGAT GGTGCCCTGT 
GACACAGAAT ACCCAGCCTT CGTCTCAGAG CGTACCATCA AAGAGACAAC TGGCAACATT 
GACTGCAATG GCTGTATACG GACATTTGTG ATTCAGCAGA TTCCCAGCAG TAACCTGTTT 
ATGGTGGTGG TTGAGAATAA ATGTGACTGT GGCTCTGCTC TTCCAGTTAC CATGGAGCCC 
ATTGAGATCA TTTATAATGA GTCACTGAAG TGTGACAGAT TGAAGTTTCA GAAGGATCGC 
AGACGACCAC AGTCCTGCCA TCCATTTCAT CCAGAGGAGA ATGCTGTGGA ATGTGGATCA 
GCGAATGCTT TGAGTTTCCC ACTGGTGGCC ATTTTAATCC CAGCATTGTC ATTAATGATC 
AGCAGGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna2d3 ( XM_005172923.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172980.1 Danio rerio calcium channel, voltage dependent, alpha2/delta subunit 3 (cacna2d3), transcript variant X3, mRNA.

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CloneID	ODa17277
Clone ID Related Accession (Same CDS sequence)	XM_005172923.3 , XM_005172923.4
Accession Version	XM_005172923.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent calcium channel subunit alpha-2/delta-3 isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172923.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGGA ACCGGAGAAA TCCTGCTCTG CTCTGGTGTG TGATGCTGAG CGCGTGCTGG 
ACGCCGCTGG AGGTGCGGGG GTCCCAACCG GAGATTCCCC TATCAGTGGT GAAACTCTGG 
GCTTCAGCGT TTGGTGGTGA AATAAAATCA ATAGCTTCAA AATACTCAGG ATCTCAGCTC 
TTACAGAAGA AATACAAGGA GTATGAGAAG TCGGTGCGAA TTGAGGAGAT CGACGGCGCA 
AAATTGGTGA AAAATCTGGC GCAGAACATG GAGGAGATGT TTCGGAAAAA AGCGGAGGCC 
ACACGAAGGC TCGTCGAAGC GGCAGAAGAA GCTCATCTTC AGCATGAAGA AAACCCAGAC 
CTACAGTATG AGTATTTCAA CGCAGTGCTG ATAAACGAGG TGGATGAGGA TGGGAATAGC 
GTGGAGCTGG GAGGAGAGTT TCTCCTGGAA CCCAACGATC ATTTTAATAA CCTATCCGTC 
AACCTCAGCC TCAGCGTGGT GCAGGTGCCA ACCAACATGT ACAATAAAGA TCCTGACATA 
GTCAATGGAG TTTACTGGTC GGAAGCCTTG AACAAAGTTT TCGTGGATAA CTTCAGGAGA 
GACCCAACCC TCATCTGGCA GTACTTTGGA AGCGCTAAAG GTTTTTTCCG ACAATACCCA 
GGCGTGAAAT GGTATCCTGA CGAACACGGC GTCATTGCAT TTGATTGCAG AAACAGAAAA 
TGGTATATCC AAGCAGCCAC TTCACCTAAA GATGTTGTGA TTCTAGTGGA CGTCAGTGGC 
AGTATGAAGG GTTTAAGACT GACTATAGCC AGACAGACGG TTGCTTCAAT ACTGGACACA 
CTCGGCGATG ATGACTTCTT CAACATTATT GCATACAACC AAGAGATCCA TTACGTGGAG 
CCCTGTCTAA ACGGCACTCT GGTTCAAGCT GACAGTACTA ATAAGGATCA CTTTAAAGAG 
CATCTGGACA AGTTGTTCGC CAAAGGAATC GGACTCCTGG GCAATGCACT GAGCGAGGCT 
TTCACTATCT TAAATGAGAT TAATCAGACG GGCCGTGGAA GTTCATGTAG TCAGGCGATC 
ATGCTGATAA CAGATGGAGC CACTGAAATG TACGATGATG TTTTCGCCAA ATATAACTGG 
CCTGAACGTA AGGTCCGTAT ATTCCCATAC CTGATTGGCC GTGAATCTGC ATTTGCTGAT 
AACCTAAAAT GGATGGCGTG TGCCAATAAA GGTTACTTTA GTCAGATCTC CACACTGGCT 
GACGTTCAGG AGAATGTGAT GCGGTATTTA CATGTGATGA GCAGACCTAA AGTTATCGAC 
CACGAACATG ACACAGTGTG GACTGAGGCC TACGTCGACA GCGCACAGCT TGGAGAGAAT 
AAAGGTCCAA ACCCGATGAC CACAGTGGCC ATGCCTGTGT TTAGCACCAA GAATGAAACG 
AAAAACCAAG GCATCCTGCT GGGCGTCGCG GGCACTGATA TCCCCATTCA GGAGCTAATG 
AAAGTCATCC CAAAACACAA GCTAGGAATA CACGGATATG CTTTTGCCAT CACCAGTAAT 
GGATATCTCC TGTTGCACCC TGACCTGAGG CCCCTGTACC AGGAGGGCCA GAAGAGGAAG 
AAACCCAGTT ACAGCAGTGT GGACCTGTCC GAGGTGGAGT GGGAGGACAC AGAGGATGTT 
TTGCGTACAG CGATGGTGAA CAGGAAAACT GGCACTTTCT CAATGGAAGT AAAAAGAACA 
GTGGACAAAG GGAAACGAGT CTTGACGATG CACAATGATT ATTACTACAC AGATATTAAA 
GGAACTCCAT TTAGTGTAGG AGTGGCACTC TCTCGAGGAC ATGGAAAATA CTTCTTTAAA 
GGGAATGTTT CACTAGAAGC TGGTCTACAT GATCTGGAGC AGCCGGATGT AGCCCTTGCT 
GATGAATGGA CGTACTGTAC CACAGAAGAG CATCATGAGC ATCGACACCT GACTCAAGTT 
CAGGCCATCA AAATCTACAT GACCAGCCGG AAACCACATT TCAAGTGTGA CAGAGAGCTG 
ATTCAGCAGG TTCTGTTTGA TGCTGTGGTC ACCGCTCCTG TAGAAGCCTA CTGGACTGGA 
CTGGCCCTCA ATAAATCTGA GAACTCAGAT AAAGGGGTTG AGATTGCTTT TCTCGGCACC 
CGGACTGGCC TTTCACGTAC CAACTTGTTT GTAGTTCCTG AGCAACTGAC CAATCGAGAT 
TTCCTCACTG CTGAAGATAA GGAGGGCGTC TTTAACGCTG ATCACTTTCC TCTGTGGTAT 
AAAAGAGCAG CAGAGCAGGT GCCCGGCACA TTTGTGTACT CCATTCCCTT CAGTTCAGGT 
GGTGAAAATA AGAGTGTGGT TTTGGCAAGC ACTGCTATCC AGCTTCTCGA TGACAGAAAG 
TCTCCTATTG TGGCAGCTGT GGGAATTCAA ATGAAGCTGG AATTCTTTCA GAGAAAATTC 
TGGACCGCTA GTCGGCAGTG TAATGCTCTG GATGGGAAAT GCACCATCAG TTGTGATAAT 
GAGGACATTA AATGCTATCT CATAGACAAC AATGGCTTCA TCCTGGTCTC TGAAGACACT 
TCTCAGACAA ACCTCTTCTT TGGAAAAGTG GAAGGAGCCG TCATGAATAA ACTGCTTCTG 
ATGGGCTCGT TTAAAAAGAT TAATTTGTAT GACTATCAGG CTCTGTGTAA GGAGTACGCA 
GGCAGCAGTG ACAGCGCACG CACTTTACTG GATCCCTTCA CCACTGTGAA ATGGCTCTTG 
ACAGAACTTG TCATATTTCT GCTGGAGTTT AACCTGTATA GCTGGTGGAA CTGTGACTCC 
ACAGTCAAAG CTCAGAGATC TCAGAGGACA ATGATGGTGC CCTGTGACAC AGAATACCCA 
GCCTTCGTCT CAGAGCGTAC CATCAAAGAG ACAACTGGCA ACATTGACTG CAATGGCTGT 
ATACGGACAT TTGTGATTCA GCAGATTCCC AGCAGTAACC TGTTTATGGT GGTGGTTGAG 
AATAAATGTG ACTGTGGCTC TGCTCTTCCA GTTACCATGG AGCCCATTGA GATCATTTAT 
AATGAGTCAC TGAAGTGTGA CAGATTGAAG TTTCAGAAGG ATCGCAGACG ACCACAGTCC 
TGCCATCCAT TTCATCCAGA GGAGAATGCT GTGGAATGTG GATCAGCGAA TGCTTTGAGT 
TTCCCACTGG TGGCCATTTT AATCCCAGCA TTGTCATTAA TGATCAGCAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172980.1
CDS	446..3679
Translation	MQRNRRNPALLWCVMLSACWTPLEVRGSQPEIPLSVVKLWASAFGGEIKSIASKYSGSQLLQKKYKEYEKSVRIEEIDGAKLVKNLAQNMEEMFRKKAEATRRLVEAAEEAHLQHEENPDLQYEYFNAVLINEVDEDGNSVELGGEFLLEPNDHFNNLSVNLSLSVVQVPTNMYNKDPDIVNGVYWSEALNKVFVDNFRRDPTLIWQYFGSAKGFFRQYPGVKWYPDEHGVIAFDCRNRKWYIQAATSPKDVVILVDVSGSMKGLRLTIARQTVASILDTLGDDDFFNIIAYNQEIHYVEPCLNGTLVQADSTNKDHFKEHLDKLFAKGIGLLGNALSEAFTILNEINQTGRGSSCSQAIMLITDGATEMYDDVFAKYNWPERKVRIFPYLIGRESAFADNLKWMACANKGYFSQISTLADVQENVMRYLHVMSRPKVIDHEHDTVWTEAYVDSAQLGENKGPNPMTTVAMPVFSTKNETKNQGILLGVAGTDIPIQELMKVIPKHKLGIHGYAFAITSNGYLLLHPDLRPLYQEGQKRKKPSYSSVDLSEVEWEDTEDVLRTAMVNRKTGTFSMEVKRTVDKGKRVLTMHNDYYYTDIKGTPFSVGVALSRGHGKYFFKGNVSLEAGLHDLEQPDVALADEWTYCTTEEHHEHRHLTQVQAIKIYMTSRKPHFKCDRELIQQVLFDAVVTAPVEAYWTGLALNKSENSDKGVEIAFLGTRTGLSRTNLFVVPEQLTNRDFLTAEDKEGVFNADHFPLWYKRAAEQVPGTFVYSIPFSSGGENKSVVLASTAIQLLDDRKSPIVAAVGIQMKLEFFQRKFWTASRQCNALDGKCTISCDNEDIKCYLIDNNGFILVSEDTSQTNLFFGKVEGAVMNKLLLMGSFKKINLYDYQALCKEYAGSSDSARTLLDPFTTVKWLLTELVIFLLEFNLYSWWNCDSTVKAQRSQRTMMVPCDTEYPAFVSERTIKETTGNIDCNGCIRTFVIQQIPSSNLFMVVVENKCDCGSALPVTMEPIEIIYNESLKCDRLKFQKDRRRPQSCHPFHPEENAVECGSANALSFPLVAILIPALSLMISR

Target ORF information:

RefSeq Version	XM_005172923.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage dependent, alpha2/delta subunit 3 (cacna2d3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172923.3

ORF Insert Sequence:

ATGCAGCGGA ACCGGAGAAA TCCTGCTCTG CTCTGGTGTG TGATGCTGAG CGCGTGCTGG 
ACGCCGCTGG AGGTGCGGGG GTCCCAACCG GAGATTCCCC TATCAGTGGT GAAACTCTGG 
GCTTCAGCGT TTGGTGGTGA AATAAAATCA ATAGCTTCAA AATACTCAGG ATCTCAGCTC 
TTACAGAAGA AATACAAGGA GTATGAGAAG TCGGTGCGAA TTGAGGAGAT CGACGGCGCA 
AAATTGGTGA AAAATCTGGC GCAGAACATG GAGGAGATGT TTCGGAAAAA AGCGGAGGCC 
ACACGAAGGC TCGTCGAAGC GGCAGAAGAA GCTCATCTTC AGCATGAAGA AAACCCAGAC 
CTACAGTATG AGTATTTCAA CGCAGTGCTG ATAAACGAGG TGGATGAGGA TGGGAATAGC 
GTGGAGCTGG GAGGAGAGTT TCTCCTGGAA CCCAACGATC ATTTTAATAA CCTATCCGTC 
AACCTCAGCC TCAGCGTGGT GCAGGTGCCA ACCAACATGT ACAATAAAGA TCCTGACATA 
GTCAATGGAG TTTACTGGTC GGAAGCCTTG AACAAAGTTT TCGTGGATAA CTTCAGGAGA 
GACCCAACCC TCATCTGGCA GTACTTTGGA AGCGCTAAAG GTTTTTTCCG ACAATACCCA 
GGCGTGAAAT GGTATCCTGA CGAACACGGC GTCATTGCAT TTGATTGCAG AAACAGAAAA 
TGGTATATCC AAGCAGCCAC TTCACCTAAA GATGTTGTGA TTCTAGTGGA CGTCAGTGGC 
AGTATGAAGG GTTTAAGACT GACTATAGCC AGACAGACGG TTGCTTCAAT ACTGGACACA 
CTCGGCGATG ATGACTTCTT CAACATTATT GCATACAACC AAGAGATCCA TTACGTGGAG 
CCCTGTCTAA ACGGCACTCT GGTTCAAGCT GACAGTACTA ATAAGGATCA CTTTAAAGAG 
CATCTGGACA AGTTGTTCGC CAAAGGAATC GGACTCCTGG GCAATGCACT GAGCGAGGCT 
TTCACTATCT TAAATGAGAT TAATCAGACG GGCCGTGGAA GTTCATGTAG TCAGGCGATC 
ATGCTGATAA CAGATGGAGC CACTGAAATG TACGATGATG TTTTCGCCAA ATATAACTGG 
CCTGAACGTA AGGTCCGTAT ATTCCCATAC CTGATTGGCC GTGAATCTGC ATTTGCTGAT 
AACCTAAAAT GGATGGCGTG TGCCAATAAA GGTTACTTTA GTCAGATCTC CACACTGGCT 
GACGTTCAGG AGAATGTGAT GCGGTATTTA CATGTGATGA GCAGACCTAA AGTTATCGAC 
CACGAACATG ACACAGTGTG GACTGAGGCC TACGTCGACA GCGCACAGCT TGGAGAGAAT 
AAAGGTCCAA ACCCGATGAC CACAGTGGCC ATGCCTGTGT TTAGCACCAA GAATGAAACG 
AAAAACCAAG GCATCCTGCT GGGCGTCGCG GGCACTGATA TCCCCATTCA GGAGCTAATG 
AAAGTCATCC CAAAACACAA GCTAGGAATA CACGGATATG CTTTTGCCAT CACCAGTAAT 
GGATATCTCC TGTTGCACCC TGACCTGAGG CCCCTGTACC AGGAGGGCCA GAAGAGGAAG 
AAACCCAGTT ACAGCAGTGT GGACCTGTCC GAGGTGGAGT GGGAGGACAC AGAGGATGTT 
TTGCGTACAG CGATGGTGAA CAGGAAAACT GGCACTTTCT CAATGGAAGT AAAAAGAACA 
GTGGACAAAG GGAAACGAGT CTTGACGATG CACAATGATT ATTACTACAC AGATATTAAA 
GGAACTCCAT TTAGTGTAGG AGTGGCACTC TCTCGAGGAC ATGGAAAATA CTTCTTTAAA 
GGGAATGTTT CACTAGAAGC TGGTCTACAT GATCTGGAGC AGCCGGATGT AGCCCTTGCT 
GATGAATGGA CGTACTGTAC CACAGAAGAG CATCATGAGC ATCGACACCT GACTCAAGTT 
CAGGCCATCA AAATCTACAT GACCAGCCGG AAACCACATT TCAAGTGTGA CAGAGAGCTG 
ATTCAGCAGG TTCTGTTTGA TGCTGTGGTC ACCGCTCCTG TAGAAGCCTA CTGGACTGGA 
CTGGCCCTCA ATAAATCTGA GAACTCAGAT AAAGGGGTTG AGATTGCTTT TCTCGGCACC 
CGGACTGGCC TTTCACGTAC CAACTTGTTT GTAGTTCCTG AGCAACTGAC CAATCGAGAT 
TTCCTCACTG CTGAAGATAA GGAGGGCGTC TTTAACGCTG ATCACTTTCC TCTGTGGTAT 
AAAAGAGCAG CAGAGCAGGT GCCCGGCACA TTTGTGTACT CCATTCCCTT CAGTTCAGGT 
GGTGAAAATA AGAGTGTGGT TTTGGCAAGC ACTGCTATCC AGCTTCTCGA TGACAGAAAG 
TCTCCTATTG TGGCAGCTGT GGGAATTCAA ATGAAGCTGG AATTCTTTCA GAGAAAATTC 
TGGACCGCTA GTCGGCAGTG TAATGCTCTG GATGGGAAAT GCACCATCAG TTGTGATAAT 
GAGGACATTA AATGCTATCT CATAGACAAC AATGGCTTCA TCCTGGTCTC TGAAGACACT 
TCTCAGACAA ACCTCTTCTT TGGAAAAGTG GAAGGAGCCG TCATGAATAA ACTGCTTCTG 
ATGGGCTCGT TTAAAAAGAT TAATTTGTAT GACTATCAGG CTCTGTGTAA GGAGTACGCA 
GGCAGCAGTG ACAGCGCACG CACTTTACTG GATCCCTTCA CCACTGTGAA ATGGCTCTTG 
ACAGAACTTG TCATATTTCT GCTGGAGTTT AACCTGTATA GCTGGTGGAA CTGTGACTCC 
ACAGTCAAAG CTCAGAGATC TCAGAGGACA ATGATGGTGC CCTGTGACAC AGAATACCCA 
GCCTTCGTCT CAGAGCGTAC CATCAAAGAG ACAACTGGCA ACATTGACTG CAATGGCTGT 
ATACGGACAT TTGTGATTCA GCAGATTCCC AGCAGTAACC TGTTTATGGT GGTGGTTGAG 
AATAAATGTG ACTGTGGCTC TGCTCTTCCA GTTACCATGG AGCCCATTGA GATCATTTAT 
AATGAGTCAC TGAAGTGTGA CAGATTGAAG TTTCAGAAGG ATCGCAGACG ACCACAGTCC 
TGCCATCCAT TTCATCCAGA GGAGAATGCT GTGGAATGTG GATCAGCGAA TGCTTTGAGT 
TTCCCACTGG TGGCCATTTT AATCCCAGCA TTGTCATTAA TGATCAGCAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna2d3 ( XM_005172923.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172980.1 Danio rerio calcium channel, voltage dependent, alpha2/delta subunit 3 (cacna2d3), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa17277
Clone ID Related Accession (Same CDS sequence)	XM_005172923.3 , XM_005172923.4
Accession Version	XM_005172923.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3234bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent calcium channel subunit alpha-2/delta-3 isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172923.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGGA ACCGGAGAAA TCCTGCTCTG CTCTGGTGTG TGATGCTGAG CGCGTGCTGG 
ACGCCGCTGG AGGTGCGGGG GTCCCAACCG GAGATTCCCC TATCAGTGGT GAAACTCTGG 
GCTTCAGCGT TTGGTGGTGA AATAAAATCA ATAGCTTCAA AATACTCAGG ATCTCAGCTC 
TTACAGAAGA AATACAAGGA GTATGAGAAG TCGGTGCGAA TTGAGGAGAT CGACGGCGCA 
AAATTGGTGA AAAATCTGGC GCAGAACATG GAGGAGATGT TTCGGAAAAA AGCGGAGGCC 
ACACGAAGGC TCGTCGAAGC GGCAGAAGAA GCTCATCTTC AGCATGAAGA AAACCCAGAC 
CTACAGTATG AGTATTTCAA CGCAGTGCTG ATAAACGAGG TGGATGAGGA TGGGAATAGC 
GTGGAGCTGG GAGGAGAGTT TCTCCTGGAA CCCAACGATC ATTTTAATAA CCTATCCGTC 
AACCTCAGCC TCAGCGTGGT GCAGGTGCCA ACCAACATGT ACAATAAAGA TCCTGACATA 
GTCAATGGAG TTTACTGGTC GGAAGCCTTG AACAAAGTTT TCGTGGATAA CTTCAGGAGA 
GACCCAACCC TCATCTGGCA GTACTTTGGA AGCGCTAAAG GTTTTTTCCG ACAATACCCA 
GGCGTGAAAT GGTATCCTGA CGAACACGGC GTCATTGCAT TTGATTGCAG AAACAGAAAA 
TGGTATATCC AAGCAGCCAC TTCACCTAAA GATGTTGTGA TTCTAGTGGA CGTCAGTGGC 
AGTATGAAGG GTTTAAGACT GACTATAGCC AGACAGACGG TTGCTTCAAT ACTGGACACA 
CTCGGCGATG ATGACTTCTT CAACATTATT GCATACAACC AAGAGATCCA TTACGTGGAG 
CCCTGTCTAA ACGGCACTCT GGTTCAAGCT GACAGTACTA ATAAGGATCA CTTTAAAGAG 
CATCTGGACA AGTTGTTCGC CAAAGGAATC GGACTCCTGG GCAATGCACT GAGCGAGGCT 
TTCACTATCT TAAATGAGAT TAATCAGACG GGCCGTGGAA GTTCATGTAG TCAGGCGATC 
ATGCTGATAA CAGATGGAGC CACTGAAATG TACGATGATG TTTTCGCCAA ATATAACTGG 
CCTGAACGTA AGGTCCGTAT ATTCCCATAC CTGATTGGCC GTGAATCTGC ATTTGCTGAT 
AACCTAAAAT GGATGGCGTG TGCCAATAAA GGTTACTTTA GTCAGATCTC CACACTGGCT 
GACGTTCAGG AGAATGTGAT GCGGTATTTA CATGTGATGA GCAGACCTAA AGTTATCGAC 
CACGAACATG ACACAGTGTG GACTGAGGCC TACGTCGACA GCGCACAGCT TGGAGAGAAT 
AAAGGTCCAA ACCCGATGAC CACAGTGGCC ATGCCTGTGT TTAGCACCAA GAATGAAACG 
AAAAACCAAG GCATCCTGCT GGGCGTCGCG GGCACTGATA TCCCCATTCA GGAGCTAATG 
AAAGTCATCC CAAAACACAA GCTAGGAATA CACGGATATG CTTTTGCCAT CACCAGTAAT 
GGATATCTCC TGTTGCACCC TGACCTGAGG CCCCTGTACC AGGAGGGCCA GAAGAGGAAG 
AAACCCAGTT ACAGCAGTGT GGACCTGTCC GAGGTGGAGT GGGAGGACAC AGAGGATGTT 
TTGCGTACAG CGATGGTGAA CAGGAAAACT GGCACTTTCT CAATGGAAGT AAAAAGAACA 
GTGGACAAAG GGAAACGAGT CTTGACGATG CACAATGATT ATTACTACAC AGATATTAAA 
GGAACTCCAT TTAGTGTAGG AGTGGCACTC TCTCGAGGAC ATGGAAAATA CTTCTTTAAA 
GGGAATGTTT CACTAGAAGC TGGTCTACAT GATCTGGAGC AGCCGGATGT AGCCCTTGCT 
GATGAATGGA CGTACTGTAC CACAGAAGAG CATCATGAGC ATCGACACCT GACTCAAGTT 
CAGGCCATCA AAATCTACAT GACCAGCCGG AAACCACATT TCAAGTGTGA CAGAGAGCTG 
ATTCAGCAGG TTCTGTTTGA TGCTGTGGTC ACCGCTCCTG TAGAAGCCTA CTGGACTGGA 
CTGGCCCTCA ATAAATCTGA GAACTCAGAT AAAGGGGTTG AGATTGCTTT TCTCGGCACC 
CGGACTGGCC TTTCACGTAC CAACTTGTTT GTAGTTCCTG AGCAACTGAC CAATCGAGAT 
TTCCTCACTG CTGAAGATAA GGAGGGCGTC TTTAACGCTG ATCACTTTCC TCTGTGGTAT 
AAAAGAGCAG CAGAGCAGGT GCCCGGCACA TTTGTGTACT CCATTCCCTT CAGTTCAGGT 
GGTGAAAATA AGAGTGTGGT TTTGGCAAGC ACTGCTATCC AGCTTCTCGA TGACAGAAAG 
TCTCCTATTG TGGCAGCTGT GGGAATTCAA ATGAAGCTGG AATTCTTTCA GAGAAAATTC 
TGGACCGCTA GTCGGCAGTG TAATGCTCTG GATGGGAAAT GCACCATCAG TTGTGATAAT 
GAGGACATTA AATGCTATCT CATAGACAAC AATGGCTTCA TCCTGGTCTC TGAAGACACT 
TCTCAGACAA ACCTCTTCTT TGGAAAAGTG GAAGGAGCCG TCATGAATAA ACTGCTTCTG 
ATGGGCTCGT TTAAAAAGAT TAATTTGTAT GACTATCAGG CTCTGTGTAA GGAGTACGCA 
GGCAGCAGTG ACAGCGCACG CACTTTACTG GATCCCTTCA CCACTGTGAA ATGGCTCTTG 
ACAGAACTTG TCATATTTCT GCTGGAGTTT AACCTGTATA GCTGGTGGAA CTGTGACTCC 
ACAGTCAAAG CTCAGAGATC TCAGAGGACA ATGATGGTGC CCTGTGACAC AGAATACCCA 
GCCTTCGTCT CAGAGCGTAC CATCAAAGAG ACAACTGGCA ACATTGACTG CAATGGCTGT 
ATACGGACAT TTGTGATTCA GCAGATTCCC AGCAGTAACC TGTTTATGGT GGTGGTTGAG 
AATAAATGTG ACTGTGGCTC TGCTCTTCCA GTTACCATGG AGCCCATTGA GATCATTTAT 
AATGAGTCAC TGAAGTGTGA CAGATTGAAG TTTCAGAAGG ATCGCAGACG ACCACAGTCC 
TGCCATCCAT TTCATCCAGA GGAGAATGCT GTGGAATGTG GATCAGCGAA TGCTTTGAGT 
TTCCCACTGG TGGCCATTTT AATCCCAGCA TTGTCATTAA TGATCAGCAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172980.1
CDS	543..3776
Translation	MQRNRRNPALLWCVMLSACWTPLEVRGSQPEIPLSVVKLWASAFGGEIKSIASKYSGSQLLQKKYKEYEKSVRIEEIDGAKLVKNLAQNMEEMFRKKAEATRRLVEAAEEAHLQHEENPDLQYEYFNAVLINEVDEDGNSVELGGEFLLEPNDHFNNLSVNLSLSVVQVPTNMYNKDPDIVNGVYWSEALNKVFVDNFRRDPTLIWQYFGSAKGFFRQYPGVKWYPDEHGVIAFDCRNRKWYIQAATSPKDVVILVDVSGSMKGLRLTIARQTVASILDTLGDDDFFNIIAYNQEIHYVEPCLNGTLVQADSTNKDHFKEHLDKLFAKGIGLLGNALSEAFTILNEINQTGRGSSCSQAIMLITDGATEMYDDVFAKYNWPERKVRIFPYLIGRESAFADNLKWMACANKGYFSQISTLADVQENVMRYLHVMSRPKVIDHEHDTVWTEAYVDSAQLGENKGPNPMTTVAMPVFSTKNETKNQGILLGVAGTDIPIQELMKVIPKHKLGIHGYAFAITSNGYLLLHPDLRPLYQEGQKRKKPSYSSVDLSEVEWEDTEDVLRTAMVNRKTGTFSMEVKRTVDKGKRVLTMHNDYYYTDIKGTPFSVGVALSRGHGKYFFKGNVSLEAGLHDLEQPDVALADEWTYCTTEEHHEHRHLTQVQAIKIYMTSRKPHFKCDRELIQQVLFDAVVTAPVEAYWTGLALNKSENSDKGVEIAFLGTRTGLSRTNLFVVPEQLTNRDFLTAEDKEGVFNADHFPLWYKRAAEQVPGTFVYSIPFSSGGENKSVVLASTAIQLLDDRKSPIVAAVGIQMKLEFFQRKFWTASRQCNALDGKCTISCDNEDIKCYLIDNNGFILVSEDTSQTNLFFGKVEGAVMNKLLLMGSFKKINLYDYQALCKEYAGSSDSARTLLDPFTTVKWLLTELVIFLLEFNLYSWWNCDSTVKAQRSQRTMMVPCDTEYPAFVSERTIKETTGNIDCNGCIRTFVIQQIPSSNLFMVVVENKCDCGSALPVTMEPIEIIYNESLKCDRLKFQKDRRRPQSCHPFHPEENAVECGSANALSFPLVAILIPALSLMISR

Target ORF information:

RefSeq Version	XM_005172923.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage dependent, alpha2/delta subunit 3 (cacna2d3), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172923.4

ORF Insert Sequence:

ATGCAGCGGA ACCGGAGAAA TCCTGCTCTG CTCTGGTGTG TGATGCTGAG CGCGTGCTGG 
ACGCCGCTGG AGGTGCGGGG GTCCCAACCG GAGATTCCCC TATCAGTGGT GAAACTCTGG 
GCTTCAGCGT TTGGTGGTGA AATAAAATCA ATAGCTTCAA AATACTCAGG ATCTCAGCTC 
TTACAGAAGA AATACAAGGA GTATGAGAAG TCGGTGCGAA TTGAGGAGAT CGACGGCGCA 
AAATTGGTGA AAAATCTGGC GCAGAACATG GAGGAGATGT TTCGGAAAAA AGCGGAGGCC 
ACACGAAGGC TCGTCGAAGC GGCAGAAGAA GCTCATCTTC AGCATGAAGA AAACCCAGAC 
CTACAGTATG AGTATTTCAA CGCAGTGCTG ATAAACGAGG TGGATGAGGA TGGGAATAGC 
GTGGAGCTGG GAGGAGAGTT TCTCCTGGAA CCCAACGATC ATTTTAATAA CCTATCCGTC 
AACCTCAGCC TCAGCGTGGT GCAGGTGCCA ACCAACATGT ACAATAAAGA TCCTGACATA 
GTCAATGGAG TTTACTGGTC GGAAGCCTTG AACAAAGTTT TCGTGGATAA CTTCAGGAGA 
GACCCAACCC TCATCTGGCA GTACTTTGGA AGCGCTAAAG GTTTTTTCCG ACAATACCCA 
GGCGTGAAAT GGTATCCTGA CGAACACGGC GTCATTGCAT TTGATTGCAG AAACAGAAAA 
TGGTATATCC AAGCAGCCAC TTCACCTAAA GATGTTGTGA TTCTAGTGGA CGTCAGTGGC 
AGTATGAAGG GTTTAAGACT GACTATAGCC AGACAGACGG TTGCTTCAAT ACTGGACACA 
CTCGGCGATG ATGACTTCTT CAACATTATT GCATACAACC AAGAGATCCA TTACGTGGAG 
CCCTGTCTAA ACGGCACTCT GGTTCAAGCT GACAGTACTA ATAAGGATCA CTTTAAAGAG 
CATCTGGACA AGTTGTTCGC CAAAGGAATC GGACTCCTGG GCAATGCACT GAGCGAGGCT 
TTCACTATCT TAAATGAGAT TAATCAGACG GGCCGTGGAA GTTCATGTAG TCAGGCGATC 
ATGCTGATAA CAGATGGAGC CACTGAAATG TACGATGATG TTTTCGCCAA ATATAACTGG 
CCTGAACGTA AGGTCCGTAT ATTCCCATAC CTGATTGGCC GTGAATCTGC ATTTGCTGAT 
AACCTAAAAT GGATGGCGTG TGCCAATAAA GGTTACTTTA GTCAGATCTC CACACTGGCT 
GACGTTCAGG AGAATGTGAT GCGGTATTTA CATGTGATGA GCAGACCTAA AGTTATCGAC 
CACGAACATG ACACAGTGTG GACTGAGGCC TACGTCGACA GCGCACAGCT TGGAGAGAAT 
AAAGGTCCAA ACCCGATGAC CACAGTGGCC ATGCCTGTGT TTAGCACCAA GAATGAAACG 
AAAAACCAAG GCATCCTGCT GGGCGTCGCG GGCACTGATA TCCCCATTCA GGAGCTAATG 
AAAGTCATCC CAAAACACAA GCTAGGAATA CACGGATATG CTTTTGCCAT CACCAGTAAT 
GGATATCTCC TGTTGCACCC TGACCTGAGG CCCCTGTACC AGGAGGGCCA GAAGAGGAAG 
AAACCCAGTT ACAGCAGTGT GGACCTGTCC GAGGTGGAGT GGGAGGACAC AGAGGATGTT 
TTGCGTACAG CGATGGTGAA CAGGAAAACT GGCACTTTCT CAATGGAAGT AAAAAGAACA 
GTGGACAAAG GGAAACGAGT CTTGACGATG CACAATGATT ATTACTACAC AGATATTAAA 
GGAACTCCAT TTAGTGTAGG AGTGGCACTC TCTCGAGGAC ATGGAAAATA CTTCTTTAAA 
GGGAATGTTT CACTAGAAGC TGGTCTACAT GATCTGGAGC AGCCGGATGT AGCCCTTGCT 
GATGAATGGA CGTACTGTAC CACAGAAGAG CATCATGAGC ATCGACACCT GACTCAAGTT 
CAGGCCATCA AAATCTACAT GACCAGCCGG AAACCACATT TCAAGTGTGA CAGAGAGCTG 
ATTCAGCAGG TTCTGTTTGA TGCTGTGGTC ACCGCTCCTG TAGAAGCCTA CTGGACTGGA 
CTGGCCCTCA ATAAATCTGA GAACTCAGAT AAAGGGGTTG AGATTGCTTT TCTCGGCACC 
CGGACTGGCC TTTCACGTAC CAACTTGTTT GTAGTTCCTG AGCAACTGAC CAATCGAGAT 
TTCCTCACTG CTGAAGATAA GGAGGGCGTC TTTAACGCTG ATCACTTTCC TCTGTGGTAT 
AAAAGAGCAG CAGAGCAGGT GCCCGGCACA TTTGTGTACT CCATTCCCTT CAGTTCAGGT 
GGTGAAAATA AGAGTGTGGT TTTGGCAAGC ACTGCTATCC AGCTTCTCGA TGACAGAAAG 
TCTCCTATTG TGGCAGCTGT GGGAATTCAA ATGAAGCTGG AATTCTTTCA GAGAAAATTC 
TGGACCGCTA GTCGGCAGTG TAATGCTCTG GATGGGAAAT GCACCATCAG TTGTGATAAT 
GAGGACATTA AATGCTATCT CATAGACAAC AATGGCTTCA TCCTGGTCTC TGAAGACACT 
TCTCAGACAA ACCTCTTCTT TGGAAAAGTG GAAGGAGCCG TCATGAATAA ACTGCTTCTG 
ATGGGCTCGT TTAAAAAGAT TAATTTGTAT GACTATCAGG CTCTGTGTAA GGAGTACGCA 
GGCAGCAGTG ACAGCGCACG CACTTTACTG GATCCCTTCA CCACTGTGAA ATGGCTCTTG 
ACAGAACTTG TCATATTTCT GCTGGAGTTT AACCTGTATA GCTGGTGGAA CTGTGACTCC 
ACAGTCAAAG CTCAGAGATC TCAGAGGACA ATGATGGTGC CCTGTGACAC AGAATACCCA 
GCCTTCGTCT CAGAGCGTAC CATCAAAGAG ACAACTGGCA ACATTGACTG CAATGGCTGT 
ATACGGACAT TTGTGATTCA GCAGATTCCC AGCAGTAACC TGTTTATGGT GGTGGTTGAG 
AATAAATGTG ACTGTGGCTC TGCTCTTCCA GTTACCATGG AGCCCATTGA GATCATTTAT 
AATGAGTCAC TGAAGTGTGA CAGATTGAAG TTTCAGAAGG ATCGCAGACG ACCACAGTCC 
TGCCATCCAT TTCATCCAGA GGAGAATGCT GTGGAATGTG GATCAGCGAA TGCTTTGAGT 
TTCCCACTGG TGGCCATTTT AATCCCAGCA TTGTCATTAA TGATCAGCAG GTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna2d3 ( XM_005172924.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172981.1 Danio rerio calcium channel, voltage dependent, alpha2/delta subunit 3 (cacna2d3), transcript variant X4, mRNA.

Price & Availability↑

CloneID	ODa17278
Clone ID Related Accession (Same CDS sequence)	XM_005172924.3 , XM_005172924.4
Accession Version	XM_005172924.3	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	voltage-dependent calcium channel subunit alpha-2/delta-3 isoform X4
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_005172924.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGGA ACCGGAGAAA TCCTGCTCTG CTCTGGTGTG TGATGCTGAG CGCGTGCTGG 
ACGCCGCTGG AGGTGCGGGG GTCCCAACCG GAGATTCCCC TATCAGTGGT GAAACTCTGG 
GCTTCAGCGT TTGGTGGTGA AATAAAATCA ATAGCTTCAA AATACTCAGG ATCTCAGCTC 
TTACAGAAGA AATACAAGGA GTATGAGAAG TCGGTGCGAA TTGAGGAGAT CGACGGCGCA 
AAATTGGTGA AAAATCTGGC GCAGAACATG GAGGAGATGT TTCGGAAAAA AGCGGAGGCC 
ACACGAAGGC TCGTCGAAGC GGCAGAAGAA GCTCATCTTC AGCATGAAGA AAACCCAGAC 
CTACAGTATG AGTATTTCAA CGCAGTGCTG ATAAACGAGG TGGATGAGGA TGGGAATAGC 
GTGGAGCTGG GAGGAGAGTT TCTCCTGGAA CCCAACGATC ATTTTAATAA CCTATCCGTC 
AACCTCAGCC TCAGCGTGGT GCAGGTGCCA ACCAACATGT ACAATAAAGA TCCTGACATA 
GTCAATGGAG TTTACTGGTC GGAAGCCTTG AACAAAGTTT TCGTGGATAA CTTCAGGAGA 
GACCCAACCC TCATCTGGCA GTACTTTGGA AGCGCTAAAG GTTTTTTCCG ACAATACCCA 
GGCGTGAAAT GGTATCCTGA CGAACACGGC GTCATTGCAT TTGATTGCAG AAACAGAAAA 
TGGTATATCC AAGCAGCCAC TTCACCTAAA GATGTTGTGA TTCTAGTGGA CGTCAGTGGC 
AGTATGAAGG GTTTAAGACT GACTATAGCC AGACAGACGG TTGCTTCAAT ACTGGACACA 
CTCGGCGATG ATGACTTCTT CAACATTATT GCATACAACC AAGAGATCCA TTACGTGGAG 
CCCTGTCTAA ACGGCACTCT GGTTCAAGCT GACAGTACTA ATAAGGATCA CTTTAAAGAG 
CATCTGGACA AGTTGTTCGC CAAAGGAATC GGACTCCTGG GCAATGCACT GAGCGAGGCT 
TTCACTATCT TAAATGAGAT TAATCAGACG GGCCGTGGAA GTTCATGTAG TCAGGCGATC 
ATGCTGATAA CAGATGGAGC CACTGAAATG TACGATGATG TTTTCGCCAA ATATAACTGG 
CCTGAACGTA AGGTCCGTAT ATTCCCATAC CTGATTGGCC GTGAATCTGC ATTTGCTGAT 
AACCTAAAAT GGATGGCGTG TGCCAATAAA GGTTACTTTA GTCAGATCTC CACACTGGCT 
GACGTTCAGG AGAATGTGAT GCGGTATTTA CATGTGATGA GCAGACCTAA AGTTATCGAC 
CACGAACATG ACACAGTGTG GACTGAGGCC TACGTCGACA GCGCACTTGG AGAGAATAAA 
GGTCCAAACC CGATGACCAC AGTGGCCATG CCTGTGTTTA GCACCAAGAA TGAAACGAAA 
AACCAAGGCA TCCTGCTGGG CGTCGCGGGC ACTGATATCC CCATTCAGGA GCTAATGAAA 
GTCATCCCAA AACACAAGCT AGGAATACAC GGATATGCTT TTGCCATCAC CAGTAATGGA 
TATCTCCTGT TGCACCCTGA CCTGAGGCCC CTGTACCAGG AGGGCCAGAA GAGGAAGAAA 
CCCAGTTACA GCAGTGTGGA CCTGTCCGAG GTGGAGTGGG AGGACACAGA GGATGTTTTG 
CGTACAGCGA TGGTGAACAG GAAAACTGGC ACTTTCTCAA TGGAAGTAAA AAGAACAGTG 
GACAAAGGGA AACGAGTCTT GACGATGCAC AATGATTATT ACTACACAGA TATTAAAGGA 
ACTCCATTTA GTGTAGGAGT GGCACTCTCT CGAGGACATG GAAAATACTT CTTTAAAGGG 
AATGTTTCAC TAGAAGCTGG TCTACATGAT CTGGAGCAGC CGGATGTAGC CCTTGCTGAT 
GAATGGACGT ACTGTACCAC AGAAGAGCAT CATGAGCATC GACACCTGAC TCAAGTTCAG 
GCCATCAAAA TCTACATGAC CAGCCGGAAA CCACATTTCA AGTGTGACAG AGAGCTGATT 
CAGCAGGTTC TGTTTGATGC TGTGGTCACC GCTCCTGTAG AAGCCTACTG GACTGGACTG 
GCCCTCAATA AATCTGAGAA CTCAGATAAA GGGGTTGAGA TTGCTTTTCT CGGCACCCGG 
ACTGGCCTTT CACGTACCAA CTTGTTTGTA GTTCCTGAGC AACTGACCAA TCGAGATTTC 
CTCACTGCTG AAGATAAGGA GGGCGTCTTT AACGCTGATC ACTTTCCTCT GTGGTATAAA 
AGAGCAGCAG AGCAGGTGCC CGGCACATTT GTGTACTCCA TTCCCTTCAG TTCAGGTGGT 
GAAAATAAGA GTGTGGTTTT GGCAAGCACT GCTATCCAGC TTCTCGATGA CAGAAAGTCT 
CCTATTGTGG CAGCTGTGGG AATTCAAATG AAGCTGGAAT TCTTTCAGAG AAAATTCTGG 
ACCGCTAGTC GGCAGTGTAA TGCTCTGGAT GGGAAATGCA CCATCAGTTG TGATAATGAG 
GACATTAAAT GCTATCTCAT AGACAACAAT GGCTTCATCC TGGTCTCTGA AGACACTTCT 
CAGACAAACC TCTTCTTTGG AAAAGTGGAA GGAGCCGTCA TGAATAAACT GCTTCTGATG 
GGCTCGTTTA AAAAGATTAA TTTGTATGAC TATCAGGCTC TGTGTAAGGA GTACGCAGGC 
AGCAGTGACA GCGCACGCAC TTTACTGGAT CCCTTCACCA CTGTGAAATG GCTCTTGACA 
GAACTTGTCA TATTTCTGCT GGAGTTTAAC CTGTATAGCT GGTGGAACTG TGACTCCACA 
GTCAAAGCTC AGAGATCTCA GAGGACAATG ATGGTGCCCT GTGACACAGA ATACCCAGCC 
TTCGTCTCAG AGCGTACCAT CAAAGAGACA ACTGGCAACA TTGACTGCAA TGGCTGTATA 
CGGACATTTG TGATTCAGCA GATTCCCAGC AGTAACCTGT TTATGGTGGT GGTTGAGAAT 
AAATGTGACT GTGGCTCTGC TCTTCCAGTT ACCATGGAGC CCATTGAGAT CATTTATAAT 
GAGTCACTGA AGTGTGACAG ATTGAAGTTT CAGAAGGATC GCAGACGACC ACAGTCCTGC 
CATCCATTTC ATCCAGAGGA GAATGCTGTG GAATGTGGAT CAGCGAATGC TTTGAGTTTC 
CCACTGGTGG CCATTTTAAT CCCAGCATTG TCATTAATGA TCAGCAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172981.1
CDS	447..3677
Translation	MQRNRRNPALLWCVMLSACWTPLEVRGSQPEIPLSVVKLWASAFGGEIKSIASKYSGSQLLQKKYKEYEKSVRIEEIDGAKLVKNLAQNMEEMFRKKAEATRRLVEAAEEAHLQHEENPDLQYEYFNAVLINEVDEDGNSVELGGEFLLEPNDHFNNLSVNLSLSVVQVPTNMYNKDPDIVNGVYWSEALNKVFVDNFRRDPTLIWQYFGSAKGFFRQYPGVKWYPDEHGVIAFDCRNRKWYIQAATSPKDVVILVDVSGSMKGLRLTIARQTVASILDTLGDDDFFNIIAYNQEIHYVEPCLNGTLVQADSTNKDHFKEHLDKLFAKGIGLLGNALSEAFTILNEINQTGRGSSCSQAIMLITDGATEMYDDVFAKYNWPERKVRIFPYLIGRESAFADNLKWMACANKGYFSQISTLADVQENVMRYLHVMSRPKVIDHEHDTVWTEAYVDSALGENKGPNPMTTVAMPVFSTKNETKNQGILLGVAGTDIPIQELMKVIPKHKLGIHGYAFAITSNGYLLLHPDLRPLYQEGQKRKKPSYSSVDLSEVEWEDTEDVLRTAMVNRKTGTFSMEVKRTVDKGKRVLTMHNDYYYTDIKGTPFSVGVALSRGHGKYFFKGNVSLEAGLHDLEQPDVALADEWTYCTTEEHHEHRHLTQVQAIKIYMTSRKPHFKCDRELIQQVLFDAVVTAPVEAYWTGLALNKSENSDKGVEIAFLGTRTGLSRTNLFVVPEQLTNRDFLTAEDKEGVFNADHFPLWYKRAAEQVPGTFVYSIPFSSGGENKSVVLASTAIQLLDDRKSPIVAAVGIQMKLEFFQRKFWTASRQCNALDGKCTISCDNEDIKCYLIDNNGFILVSEDTSQTNLFFGKVEGAVMNKLLLMGSFKKINLYDYQALCKEYAGSSDSARTLLDPFTTVKWLLTELVIFLLEFNLYSWWNCDSTVKAQRSQRTMMVPCDTEYPAFVSERTIKETTGNIDCNGCIRTFVIQQIPSSNLFMVVVENKCDCGSALPVTMEPIEIIYNESLKCDRLKFQKDRRRPQSCHPFHPEENAVECGSANALSFPLVAILIPALSLMISR

Target ORF information:

RefSeq Version	XM_005172924.3
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio calcium channel, voltage dependent, alpha2/delta subunit 3 (cacna2d3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172924.3

ORF Insert Sequence:

ATGCAGCGGA ACCGGAGAAA TCCTGCTCTG CTCTGGTGTG TGATGCTGAG CGCGTGCTGG 
ACGCCGCTGG AGGTGCGGGG GTCCCAACCG GAGATTCCCC TATCAGTGGT GAAACTCTGG 
GCTTCAGCGT TTGGTGGTGA AATAAAATCA ATAGCTTCAA AATACTCAGG ATCTCAGCTC 
TTACAGAAGA AATACAAGGA GTATGAGAAG TCGGTGCGAA TTGAGGAGAT CGACGGCGCA 
AAATTGGTGA AAAATCTGGC GCAGAACATG GAGGAGATGT TTCGGAAAAA AGCGGAGGCC 
ACACGAAGGC TCGTCGAAGC GGCAGAAGAA GCTCATCTTC AGCATGAAGA AAACCCAGAC 
CTACAGTATG AGTATTTCAA CGCAGTGCTG ATAAACGAGG TGGATGAGGA TGGGAATAGC 
GTGGAGCTGG GAGGAGAGTT TCTCCTGGAA CCCAACGATC ATTTTAATAA CCTATCCGTC 
AACCTCAGCC TCAGCGTGGT GCAGGTGCCA ACCAACATGT ACAATAAAGA TCCTGACATA 
GTCAATGGAG TTTACTGGTC GGAAGCCTTG AACAAAGTTT TCGTGGATAA CTTCAGGAGA 
GACCCAACCC TCATCTGGCA GTACTTTGGA AGCGCTAAAG GTTTTTTCCG ACAATACCCA 
GGCGTGAAAT GGTATCCTGA CGAACACGGC GTCATTGCAT TTGATTGCAG AAACAGAAAA 
TGGTATATCC AAGCAGCCAC TTCACCTAAA GATGTTGTGA TTCTAGTGGA CGTCAGTGGC 
AGTATGAAGG GTTTAAGACT GACTATAGCC AGACAGACGG TTGCTTCAAT ACTGGACACA 
CTCGGCGATG ATGACTTCTT CAACATTATT GCATACAACC AAGAGATCCA TTACGTGGAG 
CCCTGTCTAA ACGGCACTCT GGTTCAAGCT GACAGTACTA ATAAGGATCA CTTTAAAGAG 
CATCTGGACA AGTTGTTCGC CAAAGGAATC GGACTCCTGG GCAATGCACT GAGCGAGGCT 
TTCACTATCT TAAATGAGAT TAATCAGACG GGCCGTGGAA GTTCATGTAG TCAGGCGATC 
ATGCTGATAA CAGATGGAGC CACTGAAATG TACGATGATG TTTTCGCCAA ATATAACTGG 
CCTGAACGTA AGGTCCGTAT ATTCCCATAC CTGATTGGCC GTGAATCTGC ATTTGCTGAT 
AACCTAAAAT GGATGGCGTG TGCCAATAAA GGTTACTTTA GTCAGATCTC CACACTGGCT 
GACGTTCAGG AGAATGTGAT GCGGTATTTA CATGTGATGA GCAGACCTAA AGTTATCGAC 
CACGAACATG ACACAGTGTG GACTGAGGCC TACGTCGACA GCGCACTTGG AGAGAATAAA 
GGTCCAAACC CGATGACCAC AGTGGCCATG CCTGTGTTTA GCACCAAGAA TGAAACGAAA 
AACCAAGGCA TCCTGCTGGG CGTCGCGGGC ACTGATATCC CCATTCAGGA GCTAATGAAA 
GTCATCCCAA AACACAAGCT AGGAATACAC GGATATGCTT TTGCCATCAC CAGTAATGGA 
TATCTCCTGT TGCACCCTGA CCTGAGGCCC CTGTACCAGG AGGGCCAGAA GAGGAAGAAA 
CCCAGTTACA GCAGTGTGGA CCTGTCCGAG GTGGAGTGGG AGGACACAGA GGATGTTTTG 
CGTACAGCGA TGGTGAACAG GAAAACTGGC ACTTTCTCAA TGGAAGTAAA AAGAACAGTG 
GACAAAGGGA AACGAGTCTT GACGATGCAC AATGATTATT ACTACACAGA TATTAAAGGA 
ACTCCATTTA GTGTAGGAGT GGCACTCTCT CGAGGACATG GAAAATACTT CTTTAAAGGG 
AATGTTTCAC TAGAAGCTGG TCTACATGAT CTGGAGCAGC CGGATGTAGC CCTTGCTGAT 
GAATGGACGT ACTGTACCAC AGAAGAGCAT CATGAGCATC GACACCTGAC TCAAGTTCAG 
GCCATCAAAA TCTACATGAC CAGCCGGAAA CCACATTTCA AGTGTGACAG AGAGCTGATT 
CAGCAGGTTC TGTTTGATGC TGTGGTCACC GCTCCTGTAG AAGCCTACTG GACTGGACTG 
GCCCTCAATA AATCTGAGAA CTCAGATAAA GGGGTTGAGA TTGCTTTTCT CGGCACCCGG 
ACTGGCCTTT CACGTACCAA CTTGTTTGTA GTTCCTGAGC AACTGACCAA TCGAGATTTC 
CTCACTGCTG AAGATAAGGA GGGCGTCTTT AACGCTGATC ACTTTCCTCT GTGGTATAAA 
AGAGCAGCAG AGCAGGTGCC CGGCACATTT GTGTACTCCA TTCCCTTCAG TTCAGGTGGT 
GAAAATAAGA GTGTGGTTTT GGCAAGCACT GCTATCCAGC TTCTCGATGA CAGAAAGTCT 
CCTATTGTGG CAGCTGTGGG AATTCAAATG AAGCTGGAAT TCTTTCAGAG AAAATTCTGG 
ACCGCTAGTC GGCAGTGTAA TGCTCTGGAT GGGAAATGCA CCATCAGTTG TGATAATGAG 
GACATTAAAT GCTATCTCAT AGACAACAAT GGCTTCATCC TGGTCTCTGA AGACACTTCT 
CAGACAAACC TCTTCTTTGG AAAAGTGGAA GGAGCCGTCA TGAATAAACT GCTTCTGATG 
GGCTCGTTTA AAAAGATTAA TTTGTATGAC TATCAGGCTC TGTGTAAGGA GTACGCAGGC 
AGCAGTGACA GCGCACGCAC TTTACTGGAT CCCTTCACCA CTGTGAAATG GCTCTTGACA 
GAACTTGTCA TATTTCTGCT GGAGTTTAAC CTGTATAGCT GGTGGAACTG TGACTCCACA 
GTCAAAGCTC AGAGATCTCA GAGGACAATG ATGGTGCCCT GTGACACAGA ATACCCAGCC 
TTCGTCTCAG AGCGTACCAT CAAAGAGACA ACTGGCAACA TTGACTGCAA TGGCTGTATA 
CGGACATTTG TGATTCAGCA GATTCCCAGC AGTAACCTGT TTATGGTGGT GGTTGAGAAT 
AAATGTGACT GTGGCTCTGC TCTTCCAGTT ACCATGGAGC CCATTGAGAT CATTTATAAT 
GAGTCACTGA AGTGTGACAG ATTGAAGTTT CAGAAGGATC GCAGACGACC ACAGTCCTGC 
CATCCATTTC ATCCAGAGGA GAATGCTGTG GAATGTGGAT CAGCGAATGC TTTGAGTTTC 
CCACTGGTGG CCATTTTAAT CCCAGCATTG TCATTAATGA TCAGCAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

cacna2d3 ( XM_005172924.4 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_005172981.1 Danio rerio calcium channel, voltage dependent, alpha2/delta subunit 3 (cacna2d3), transcript variant X4, mRNA.

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CloneID	ODa17278
Clone ID Related Accession (Same CDS sequence)	XM_005172924.3 , XM_005172924.4
Accession Version	XM_005172924.4 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3231bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	voltage-dependent calcium channel subunit alpha-2/delta-3 isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007122.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_005172924.3. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGCGGA ACCGGAGAAA TCCTGCTCTG CTCTGGTGTG TGATGCTGAG CGCGTGCTGG 
ACGCCGCTGG AGGTGCGGGG GTCCCAACCG GAGATTCCCC TATCAGTGGT GAAACTCTGG 
GCTTCAGCGT TTGGTGGTGA AATAAAATCA ATAGCTTCAA AATACTCAGG ATCTCAGCTC 
TTACAGAAGA AATACAAGGA GTATGAGAAG TCGGTGCGAA TTGAGGAGAT CGACGGCGCA 
AAATTGGTGA AAAATCTGGC GCAGAACATG GAGGAGATGT TTCGGAAAAA AGCGGAGGCC 
ACACGAAGGC TCGTCGAAGC GGCAGAAGAA GCTCATCTTC AGCATGAAGA AAACCCAGAC 
CTACAGTATG AGTATTTCAA CGCAGTGCTG ATAAACGAGG TGGATGAGGA TGGGAATAGC 
GTGGAGCTGG GAGGAGAGTT TCTCCTGGAA CCCAACGATC ATTTTAATAA CCTATCCGTC 
AACCTCAGCC TCAGCGTGGT GCAGGTGCCA ACCAACATGT ACAATAAAGA TCCTGACATA 
GTCAATGGAG TTTACTGGTC GGAAGCCTTG AACAAAGTTT TCGTGGATAA CTTCAGGAGA 
GACCCAACCC TCATCTGGCA GTACTTTGGA AGCGCTAAAG GTTTTTTCCG ACAATACCCA 
GGCGTGAAAT GGTATCCTGA CGAACACGGC GTCATTGCAT TTGATTGCAG AAACAGAAAA 
TGGTATATCC AAGCAGCCAC TTCACCTAAA GATGTTGTGA TTCTAGTGGA CGTCAGTGGC 
AGTATGAAGG GTTTAAGACT GACTATAGCC AGACAGACGG TTGCTTCAAT ACTGGACACA 
CTCGGCGATG ATGACTTCTT CAACATTATT GCATACAACC AAGAGATCCA TTACGTGGAG 
CCCTGTCTAA ACGGCACTCT GGTTCAAGCT GACAGTACTA ATAAGGATCA CTTTAAAGAG 
CATCTGGACA AGTTGTTCGC CAAAGGAATC GGACTCCTGG GCAATGCACT GAGCGAGGCT 
TTCACTATCT TAAATGAGAT TAATCAGACG GGCCGTGGAA GTTCATGTAG TCAGGCGATC 
ATGCTGATAA CAGATGGAGC CACTGAAATG TACGATGATG TTTTCGCCAA ATATAACTGG 
CCTGAACGTA AGGTCCGTAT ATTCCCATAC CTGATTGGCC GTGAATCTGC ATTTGCTGAT 
AACCTAAAAT GGATGGCGTG TGCCAATAAA GGTTACTTTA GTCAGATCTC CACACTGGCT 
GACGTTCAGG AGAATGTGAT GCGGTATTTA CATGTGATGA GCAGACCTAA AGTTATCGAC 
CACGAACATG ACACAGTGTG GACTGAGGCC TACGTCGACA GCGCACTTGG AGAGAATAAA 
GGTCCAAACC CGATGACCAC AGTGGCCATG CCTGTGTTTA GCACCAAGAA TGAAACGAAA 
AACCAAGGCA TCCTGCTGGG CGTCGCGGGC ACTGATATCC CCATTCAGGA GCTAATGAAA 
GTCATCCCAA AACACAAGCT AGGAATACAC GGATATGCTT TTGCCATCAC CAGTAATGGA 
TATCTCCTGT TGCACCCTGA CCTGAGGCCC CTGTACCAGG AGGGCCAGAA GAGGAAGAAA 
CCCAGTTACA GCAGTGTGGA CCTGTCCGAG GTGGAGTGGG AGGACACAGA GGATGTTTTG 
CGTACAGCGA TGGTGAACAG GAAAACTGGC ACTTTCTCAA TGGAAGTAAA AAGAACAGTG 
GACAAAGGGA AACGAGTCTT GACGATGCAC AATGATTATT ACTACACAGA TATTAAAGGA 
ACTCCATTTA GTGTAGGAGT GGCACTCTCT CGAGGACATG GAAAATACTT CTTTAAAGGG 
AATGTTTCAC TAGAAGCTGG TCTACATGAT CTGGAGCAGC CGGATGTAGC CCTTGCTGAT 
GAATGGACGT ACTGTACCAC AGAAGAGCAT CATGAGCATC GACACCTGAC TCAAGTTCAG 
GCCATCAAAA TCTACATGAC CAGCCGGAAA CCACATTTCA AGTGTGACAG AGAGCTGATT 
CAGCAGGTTC TGTTTGATGC TGTGGTCACC GCTCCTGTAG AAGCCTACTG GACTGGACTG 
GCCCTCAATA AATCTGAGAA CTCAGATAAA GGGGTTGAGA TTGCTTTTCT CGGCACCCGG 
ACTGGCCTTT CACGTACCAA CTTGTTTGTA GTTCCTGAGC AACTGACCAA TCGAGATTTC 
CTCACTGCTG AAGATAAGGA GGGCGTCTTT AACGCTGATC ACTTTCCTCT GTGGTATAAA 
AGAGCAGCAG AGCAGGTGCC CGGCACATTT GTGTACTCCA TTCCCTTCAG TTCAGGTGGT 
GAAAATAAGA GTGTGGTTTT GGCAAGCACT GCTATCCAGC TTCTCGATGA CAGAAAGTCT 
CCTATTGTGG CAGCTGTGGG AATTCAAATG AAGCTGGAAT TCTTTCAGAG AAAATTCTGG 
ACCGCTAGTC GGCAGTGTAA TGCTCTGGAT GGGAAATGCA CCATCAGTTG TGATAATGAG 
GACATTAAAT GCTATCTCAT AGACAACAAT GGCTTCATCC TGGTCTCTGA AGACACTTCT 
CAGACAAACC TCTTCTTTGG AAAAGTGGAA GGAGCCGTCA TGAATAAACT GCTTCTGATG 
GGCTCGTTTA AAAAGATTAA TTTGTATGAC TATCAGGCTC TGTGTAAGGA GTACGCAGGC 
AGCAGTGACA GCGCACGCAC TTTACTGGAT CCCTTCACCA CTGTGAAATG GCTCTTGACA 
GAACTTGTCA TATTTCTGCT GGAGTTTAAC CTGTATAGCT GGTGGAACTG TGACTCCACA 
GTCAAAGCTC AGAGATCTCA GAGGACAATG ATGGTGCCCT GTGACACAGA ATACCCAGCC 
TTCGTCTCAG AGCGTACCAT CAAAGAGACA ACTGGCAACA TTGACTGCAA TGGCTGTATA 
CGGACATTTG TGATTCAGCA GATTCCCAGC AGTAACCTGT TTATGGTGGT GGTTGAGAAT 
AAATGTGACT GTGGCTCTGC TCTTCCAGTT ACCATGGAGC CCATTGAGAT CATTTATAAT 
GAGTCACTGA AGTGTGACAG ATTGAAGTTT CAGAAGGATC GCAGACGACC ACAGTCCTGC 
CATCCATTTC ATCCAGAGGA GAATGCTGTG GAATGTGGAT CAGCGAATGC TTTGAGTTTC 
CCACTGGTGG CCATTTTAAT CCCAGCATTG TCATTAATGA TCAGCAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_005172981.1
CDS	544..3774
Translation	MQRNRRNPALLWCVMLSACWTPLEVRGSQPEIPLSVVKLWASAFGGEIKSIASKYSGSQLLQKKYKEYEKSVRIEEIDGAKLVKNLAQNMEEMFRKKAEATRRLVEAAEEAHLQHEENPDLQYEYFNAVLINEVDEDGNSVELGGEFLLEPNDHFNNLSVNLSLSVVQVPTNMYNKDPDIVNGVYWSEALNKVFVDNFRRDPTLIWQYFGSAKGFFRQYPGVKWYPDEHGVIAFDCRNRKWYIQAATSPKDVVILVDVSGSMKGLRLTIARQTVASILDTLGDDDFFNIIAYNQEIHYVEPCLNGTLVQADSTNKDHFKEHLDKLFAKGIGLLGNALSEAFTILNEINQTGRGSSCSQAIMLITDGATEMYDDVFAKYNWPERKVRIFPYLIGRESAFADNLKWMACANKGYFSQISTLADVQENVMRYLHVMSRPKVIDHEHDTVWTEAYVDSALGENKGPNPMTTVAMPVFSTKNETKNQGILLGVAGTDIPIQELMKVIPKHKLGIHGYAFAITSNGYLLLHPDLRPLYQEGQKRKKPSYSSVDLSEVEWEDTEDVLRTAMVNRKTGTFSMEVKRTVDKGKRVLTMHNDYYYTDIKGTPFSVGVALSRGHGKYFFKGNVSLEAGLHDLEQPDVALADEWTYCTTEEHHEHRHLTQVQAIKIYMTSRKPHFKCDRELIQQVLFDAVVTAPVEAYWTGLALNKSENSDKGVEIAFLGTRTGLSRTNLFVVPEQLTNRDFLTAEDKEGVFNADHFPLWYKRAAEQVPGTFVYSIPFSSGGENKSVVLASTAIQLLDDRKSPIVAAVGIQMKLEFFQRKFWTASRQCNALDGKCTISCDNEDIKCYLIDNNGFILVSEDTSQTNLFFGKVEGAVMNKLLLMGSFKKINLYDYQALCKEYAGSSDSARTLLDPFTTVKWLLTELVIFLLEFNLYSWWNCDSTVKAQRSQRTMMVPCDTEYPAFVSERTIKETTGNIDCNGCIRTFVIQQIPSSNLFMVVVENKCDCGSALPVTMEPIEIIYNESLKCDRLKFQKDRRRPQSCHPFHPEENAVECGSANALSFPLVAILIPALSLMISR

Target ORF information:

RefSeq Version	XM_005172924.4
Organism	Danio rerio(zebrafish)
Definition	Danio rerio calcium channel, voltage dependent, alpha2/delta subunit 3 (cacna2d3), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_005172924.4

ORF Insert Sequence:

ATGCAGCGGA ACCGGAGAAA TCCTGCTCTG CTCTGGTGTG TGATGCTGAG CGCGTGCTGG 
ACGCCGCTGG AGGTGCGGGG GTCCCAACCG GAGATTCCCC TATCAGTGGT GAAACTCTGG 
GCTTCAGCGT TTGGTGGTGA AATAAAATCA ATAGCTTCAA AATACTCAGG ATCTCAGCTC 
TTACAGAAGA AATACAAGGA GTATGAGAAG TCGGTGCGAA TTGAGGAGAT CGACGGCGCA 
AAATTGGTGA AAAATCTGGC GCAGAACATG GAGGAGATGT TTCGGAAAAA AGCGGAGGCC 
ACACGAAGGC TCGTCGAAGC GGCAGAAGAA GCTCATCTTC AGCATGAAGA AAACCCAGAC 
CTACAGTATG AGTATTTCAA CGCAGTGCTG ATAAACGAGG TGGATGAGGA TGGGAATAGC 
GTGGAGCTGG GAGGAGAGTT TCTCCTGGAA CCCAACGATC ATTTTAATAA CCTATCCGTC 
AACCTCAGCC TCAGCGTGGT GCAGGTGCCA ACCAACATGT ACAATAAAGA TCCTGACATA 
GTCAATGGAG TTTACTGGTC GGAAGCCTTG AACAAAGTTT TCGTGGATAA CTTCAGGAGA 
GACCCAACCC TCATCTGGCA GTACTTTGGA AGCGCTAAAG GTTTTTTCCG ACAATACCCA 
GGCGTGAAAT GGTATCCTGA CGAACACGGC GTCATTGCAT TTGATTGCAG AAACAGAAAA 
TGGTATATCC AAGCAGCCAC TTCACCTAAA GATGTTGTGA TTCTAGTGGA CGTCAGTGGC 
AGTATGAAGG GTTTAAGACT GACTATAGCC AGACAGACGG TTGCTTCAAT ACTGGACACA 
CTCGGCGATG ATGACTTCTT CAACATTATT GCATACAACC AAGAGATCCA TTACGTGGAG 
CCCTGTCTAA ACGGCACTCT GGTTCAAGCT GACAGTACTA ATAAGGATCA CTTTAAAGAG 
CATCTGGACA AGTTGTTCGC CAAAGGAATC GGACTCCTGG GCAATGCACT GAGCGAGGCT 
TTCACTATCT TAAATGAGAT TAATCAGACG GGCCGTGGAA GTTCATGTAG TCAGGCGATC 
ATGCTGATAA CAGATGGAGC CACTGAAATG TACGATGATG TTTTCGCCAA ATATAACTGG 
CCTGAACGTA AGGTCCGTAT ATTCCCATAC CTGATTGGCC GTGAATCTGC ATTTGCTGAT 
AACCTAAAAT GGATGGCGTG TGCCAATAAA GGTTACTTTA GTCAGATCTC CACACTGGCT 
GACGTTCAGG AGAATGTGAT GCGGTATTTA CATGTGATGA GCAGACCTAA AGTTATCGAC 
CACGAACATG ACACAGTGTG GACTGAGGCC TACGTCGACA GCGCACTTGG AGAGAATAAA 
GGTCCAAACC CGATGACCAC AGTGGCCATG CCTGTGTTTA GCACCAAGAA TGAAACGAAA 
AACCAAGGCA TCCTGCTGGG CGTCGCGGGC ACTGATATCC CCATTCAGGA GCTAATGAAA 
GTCATCCCAA AACACAAGCT AGGAATACAC GGATATGCTT TTGCCATCAC CAGTAATGGA 
TATCTCCTGT TGCACCCTGA CCTGAGGCCC CTGTACCAGG AGGGCCAGAA GAGGAAGAAA 
CCCAGTTACA GCAGTGTGGA CCTGTCCGAG GTGGAGTGGG AGGACACAGA GGATGTTTTG 
CGTACAGCGA TGGTGAACAG GAAAACTGGC ACTTTCTCAA TGGAAGTAAA AAGAACAGTG 
GACAAAGGGA AACGAGTCTT GACGATGCAC AATGATTATT ACTACACAGA TATTAAAGGA 
ACTCCATTTA GTGTAGGAGT GGCACTCTCT CGAGGACATG GAAAATACTT CTTTAAAGGG 
AATGTTTCAC TAGAAGCTGG TCTACATGAT CTGGAGCAGC CGGATGTAGC CCTTGCTGAT 
GAATGGACGT ACTGTACCAC AGAAGAGCAT CATGAGCATC GACACCTGAC TCAAGTTCAG 
GCCATCAAAA TCTACATGAC CAGCCGGAAA CCACATTTCA AGTGTGACAG AGAGCTGATT 
CAGCAGGTTC TGTTTGATGC TGTGGTCACC GCTCCTGTAG AAGCCTACTG GACTGGACTG 
GCCCTCAATA AATCTGAGAA CTCAGATAAA GGGGTTGAGA TTGCTTTTCT CGGCACCCGG 
ACTGGCCTTT CACGTACCAA CTTGTTTGTA GTTCCTGAGC AACTGACCAA TCGAGATTTC 
CTCACTGCTG AAGATAAGGA GGGCGTCTTT AACGCTGATC ACTTTCCTCT GTGGTATAAA 
AGAGCAGCAG AGCAGGTGCC CGGCACATTT GTGTACTCCA TTCCCTTCAG TTCAGGTGGT 
GAAAATAAGA GTGTGGTTTT GGCAAGCACT GCTATCCAGC TTCTCGATGA CAGAAAGTCT 
CCTATTGTGG CAGCTGTGGG AATTCAAATG AAGCTGGAAT TCTTTCAGAG AAAATTCTGG 
ACCGCTAGTC GGCAGTGTAA TGCTCTGGAT GGGAAATGCA CCATCAGTTG TGATAATGAG 
GACATTAAAT GCTATCTCAT AGACAACAAT GGCTTCATCC TGGTCTCTGA AGACACTTCT 
CAGACAAACC TCTTCTTTGG AAAAGTGGAA GGAGCCGTCA TGAATAAACT GCTTCTGATG 
GGCTCGTTTA AAAAGATTAA TTTGTATGAC TATCAGGCTC TGTGTAAGGA GTACGCAGGC 
AGCAGTGACA GCGCACGCAC TTTACTGGAT CCCTTCACCA CTGTGAAATG GCTCTTGACA 
GAACTTGTCA TATTTCTGCT GGAGTTTAAC CTGTATAGCT GGTGGAACTG TGACTCCACA 
GTCAAAGCTC AGAGATCTCA GAGGACAATG ATGGTGCCCT GTGACACAGA ATACCCAGCC 
TTCGTCTCAG AGCGTACCAT CAAAGAGACA ACTGGCAACA TTGACTGCAA TGGCTGTATA 
CGGACATTTG TGATTCAGCA GATTCCCAGC AGTAACCTGT TTATGGTGGT GGTTGAGAAT 
AAATGTGACT GTGGCTCTGC TCTTCCAGTT ACCATGGAGC CCATTGAGAT CATTTATAAT 
GAGTCACTGA AGTGTGACAG ATTGAAGTTT CAGAAGGATC GCAGACGACC ACAGTCCTGC 
CATCCATTTC ATCCAGAGGA GAATGCTGTG GAATGTGGAT CAGCGAATGC TTTGAGTTTC 
CCACTGGTGG CCATTTTAAT CCCAGCATTG TCATTAATGA TCAGCAGGTG A

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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cacna2d3 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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電話

FAX

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