LOC100148041 cDNA ORF clone, Danio rerio(zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	LOC100148041
Entrez Gene ID	100148041
Full Name	activated CDC42 kinase 1-like
Gene Type	protein-coding
Organism	Danio rerio(zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_009297485.3	XP_009295760.2	activated CDC42 kinase 1-like isoform X2
XM_021470344.1	XP_021326019.1	activated CDC42 kinase 1-like isoform X1
XM_009297483.2	XP_009295758.1	activated CDC42 kinase 1-like isoform X1
XM_021470345.1	XP_021326020.1	activated CDC42 kinase 1-like isoform X2
XM_009297484.2	XP_009295759.1	activated CDC42 kinase 1-like isoform X2
XM_009297483.3	XP_009295758.2	activated CDC42 kinase 1-like isoform X1
XM_009297485.2	XP_009295760.1	activated CDC42 kinase 1-like isoform X3
XM_009297484.3	XP_009295759.2	activated CDC42 kinase 1-like isoform X3

Pathways from BioSystems

Homologies

Danio rerio (zebrafish)	LOC562887	XP_005162844.1
Danio rerio (zebrafish)	LOC100148041	XP_005162839.1

Gene Ontology

The following LOC100148041 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the LOC100148041 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa146350	XM_009297483.3 Latest version!	Danio rerio activated CDC42 kinase 1-like (LOC100148041), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa146351	XM_009297484.3 Latest version!	Danio rerio activated CDC42 kinase 1-like (LOC100148041), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa146352	XM_009297485.3 Latest version!	Danio rerio activated CDC42 kinase 1-like (LOC100148041), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00
ODa28667	XM_009297484.2 Latest version!	Danio rerio activated CDC42 kinase 1-like (LOC100148041), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa48613	XM_009297483.2 Latest version!	Danio rerio activated CDC42 kinase 1-like (LOC100148041), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa48613	XM_021470344.1 Latest version!	Danio rerio activated CDC42 kinase 1-like (LOC100148041), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa48614	XM_009297485.2 Latest version!	Danio rerio activated CDC42 kinase 1-like (LOC100148041), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00
ODa48614	XM_021470345.1 Latest version!	Danio rerio activated CDC42 kinase 1-like (LOC100148041), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	20	$629.30 $899.00

ORF Online Only Promotion

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

LOC100148041 ( XM_009297483.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295758.2 Danio rerio activated CDC42 kinase 1-like (LOC100148041), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa146350
Clone ID Related Accession (Same CDS sequence)	XM_009297483.3
Accession Version	XM_009297483.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	activated CDC42 kinase 1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395177.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297483.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC TCTCAGATTA CCAACGACAC TATAATGTAA AAGACGAGCC GGACATGCAG 
TCTGATGAAG GTACAGAGTG GCTCTTGGAG CTGCTCACAG ATGTGCAGCT TCAGCAATAT 
TTCCAGCGTA TCCGTGATGA GCTCAATGTC ACGCGTCTCT CACACTTTGA CTATGTCAAA 
ACAGAGGACC TCGAAAAGAT TGGCATGGGC CGACCAGGTC AAAGGCGGCT GTGGGAGGCA 
GTCAAAAGAA GGAGGGCAAT GTGCAAACGG AAGTCATGGA TGAGCAAGTC TCTGTGTTTG 
GGTCAGCAGG TGTTTACCGG GAAGCGCCCT GACTCCGATT CGCAGACTCC AGAGGTTTTC 
CGCAGCTCGT CCCCTTCATC GGCTCCTCCG GATGGCCAGC CGACGGATCT CACCTGCCTC 
ATCAGCGAAC GAGACCTCAC TCTCTATGAA AAACTGGGGG ACGGATCTTT CGGAGTGGTG 
AAGCGTGGAG AATGGCAGGC CCCCTCTGGA AGAGTGCTAA GCGTGGCTGT GAAGTGCCTG 
AAGACGGACC TGTTGGACCA GGGTGAAGGT CTAGATGACT TCATACGAGA AGTTAATGCC 
ATGCACTCCT TGAACCACCA CAATCTCATT CGTCTGTATG GGTGCGTCCT CACACACCCC 
ATGAAGATGG TGACAGAACT TGCTCCATTG GGTTCTCTGT TGGACCGCCT GAGGAAACGT 
CAGGGTCATA TACTGATATC AGTGCTGTGC CACTACACTG TTCAGATCGC CTGTGGGATG 
GCATACCTGG AGTCTCGACG CTTCATTCAC AGAGACCTGG CTGCACGCAA CATCCTTTTA 
GCCTCCAATG ACCTCATCAA GATCGGTGAC TTCGGCCTGA TGAGGGCCTT GCCTAAAAAT 
GATGATCATT ACGTTATGCA GGAGCATCAC AAAGTCCCTT TTGCATGGTG TGCTCCAGAG 
AGCCTGAAGA CCCGTACCTT CTCCCATGCC AGTGATACCT GGATGTTTGG AGTAACATTG 
TGGGAAATGT TCACTCACGG GCAGGAACCA TGGGTTGGAC TCAATGGTAG TCAGATCCTC 
CATAAGATCG ATAGAGAGGG AGAAAGGCTT ATCAAACCAG AGGACTGTCC TCAGGACATT 
TATAACGTCA TGCTGCAGTG CTGGTCACCC AAACCTGAGG ACAGGCCGAC ATTTGTGTCC 
CTTAGGGACT TCCTTGTGGA GACAATGCCA ACAGATATGA GGGCACTGCA GGACTTTGAT 
GAGCCAGACA AACTTAAAAT CCACGCCAAT GACATCATCA CCATCATTGA GGGCAGAGCA 
GAGAACTACT GGTGGAGGGG TCAGAACAGA CAGACCATGA AGGTGGGACA GTTCCCCAGG 
AATGTAGTGA CCTCTGTGGC CGGTCTATCT GCTCAGGACA TCAGCCGACC TCTCAAGCAC 
AGTTTCATCC ACACAGGCCA TGGAGACACT GACCCACACC GTAGCTGGGG TGCCCCTGAT 
AAGATTGATG ATCTGTACCT TGGTAATCCC ATGGATCCTC CAGATGTTCT TGGGTTGGAT 
TCAAATACAG CCAAGCCCAC TAAGCTACCA AACCGGGCTA AAAAACAACC TCCTCCTAGA 
CCTCCTAAAC CAGCTGTTCT TCTTAAGAAA CCATTTTATG ACTCTGTCCT GGATGATGAT 
GATGATGATG ATCTGATAGT GACAGGTGTG AAGAAGCTCT CACTTAAGAC TCCTACATAC 
CTGGGTTTAC GCCTTCGCCC ATGGGAAAGC ACTAGTCCGA GTGACCGCCT TTGTGAAGTC 
TCCCTTATTG ACTTTGGGGA TGATAGTTTC AGCTCAACAT CGCCCTCTCC CATTACCGAG 
ATTCCCAATC CTAGCACAGC GAAACCACCT TTGTCCTATG CCGCTTCCAT CTTGGATATG 
GCACCACCCC AGAGCCCAAA TCAAGCCTTA CCCAACCCCA TGCACCCTAC TCCTGTGGTA 
GACTGGGACA TGCGGCCCCT GCCTCCTCTC CCGGCATATG ATGAGGTGGC CATTGAGTGT 
GCAGAGCAGG AAGACATAGA AGTGAGCTCC ATAAATGCAT CTGCAGCCCA GGAAGACGCT 
GTATATGAAC AGACCCCATC AGAAGAAGAC ATTAATAATG ATGGTAAACG GAACGTGGAG 
GATAACCTCT TTCTTCCATC AAAGCGTCCT GAGGAATCCC ACTCGTTTTC ACAATCAGCA 
GATATCTTCA AGGAACTTCA AAGAGAGGTG ATGGTAAAGC TTCGGGTCCC TCCAAGTGGG 
CGTTCTCTAC CTTCTTCACC CCTCCCGACT CCTTCAGTTC TCACCCCACA CCGTCAGATA 
ATCCTTCCAT CCTCTTATGA GGACAAGCCT CAGATCCCTC CAAGAATCCC TATTCCACCT 
ATGCGGCCTT CAAAGAGGAC AGGAATATAT GGCAGCAGAC TGTCTGTTTC TCTTGGAGAA 
AATGAAGATG GGAGCCGTTG TCCACCCCAA ATACCCCCGC GGGATCATGC TCTATCTCAG 
CCCAATTCTC GGGCACCAAG TCCTATGGTG CCTCAAAGCG GCTCTCCTCA ACAAAGATCC 
AGCCTGTGCT GTGTTGGATC ATTGGGCTCC TGCTTGTCTC CATCATCATA TTCTTCTGCA 
CCATCCAGCT CGTCCACCAC ATCTTCCACA TCCACCACTA CTAGTTCTCA ATATGGATCT 
ACTGAAGTGA GCCAAAATCC AGCCAAGAGT CCTTGCATCC TGCCAATTGT TCACGGTGGT 
GTAAAGGCCA GCAGCACTCA TTACTACTTG CTACCTGAGA AACCGGCATA CTTGGACAAG 
TATGAGAAGT TCTTTAATTC GGAGGGAAAC GATGAGAAAA GGACTACAAA TATGGCTACT 
GTGAGACCCA TGGTTCAGCA GCAAGGCAAC AAATCCAATG CTTCCCCTAG CCACACCAGT 
AACAGTGGGA CCTCCAGCAG TCTGGCTTGG CCAAGCAGCT CCCAAGCAGG TTCTTACAGC 
CGTGTCACGC TGCAACAAGT ACAGGAAGCA GTACATGGGG TTACTGTAGA GGAGTGTCAA 
ACAGCCTTGC AGGCACACAA CTGGAATGCT CAGGAGGCCG TGAAGTATCT AAAGGTAGAA 
CAGTTATTCC GACTGGGTTT GAAGACTCGG CCTGAATGCA TGGAAACTCT GCAGAGATGT 
GGCTGGAATC TGGAACAGGC CAGCACCCAA ATGCTGGATT CCTATGGTCC ATCCAGGCAT 
AGGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295758.2
CDS	624..3872
Translation	MAALSDYQRHYNVKDEPDMQSDEGTEWLLELLTDVQLQQYFQRIRDELNVTRLSHFDYVKTEDLEKIGMGRPGQRRLWEAVKRRRAMCKRKSWMSKSLCLGQQVFTGKRPDSDSQTPEVFRSSSPSSAPPDGQPTDLTCLISERDLTLYEKLGDGSFGVVKRGEWQAPSGRVLSVAVKCLKTDLLDQGEGLDDFIREVNAMHSLNHHNLIRLYGCVLTHPMKMVTELAPLGSLLDRLRKRQGHILISVLCHYTVQIACGMAYLESRRFIHRDLAARNILLASNDLIKIGDFGLMRALPKNDDHYVMQEHHKVPFAWCAPESLKTRTFSHASDTWMFGVTLWEMFTHGQEPWVGLNGSQILHKIDREGERLIKPEDCPQDIYNVMLQCWSPKPEDRPTFVSLRDFLVETMPTDMRALQDFDEPDKLKIHANDIITIIEGRAENYWWRGQNRQTMKVGQFPRNVVTSVAGLSAQDISRPLKHSFIHTGHGDTDPHRSWGAPDKIDDLYLGNPMDPPDVLGLDSNTAKPTKLPNRAKKQPPPRPPKPAVLLKKPFYDSVLDDDDDDDLIVTGVKKLSLKTPTYLGLRLRPWESTSPSDRLCEVSLIDFGDDSFSSTSPSPITEIPNPSTAKPPLSYAASILDMAPPQSPNQALPNPMHPTPVVDWDMRPLPPLPAYDEVAIECAEQEDIEVSSINASAAQEDAVYEQTPSEEDINNDGKRNVEDNLFLPSKRPEESHSFSQSADIFKELQREVMVKLRVPPSGRSLPSSPLPTPSVLTPHRQIILPSSYEDKPQIPPRIPIPPMRPSKRTGIYGSRLSVSLGENEDGSRCPPQIPPRDHALSQPNSRAPSPMVPQSGSPQQRSSLCCVGSLGSCLSPSSYSSAPSSSSTTSSTSTTTSSQYGSTEVSQNPAKSPCILPIVHGGVKASSTHYYLLPEKPAYLDKYEKFFNSEGNDEKRTTNMATVRPMVQQQGNKSNASPSHTSNSGTSSSLAWPSSSQAGSYSRVTLQQVQEAVHGVTVEECQTALQAHNWNAQEAVKYLKVEQLFRLGLKTRPECMETLQRCGWNLEQASTQMLDSYGPSRHRF

Target ORF information:

RefSeq Version	XM_009297483.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio activated CDC42 kinase 1-like (LOC100148041), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297483.3

ORF Insert Sequence:

ATGGCGGCGC TCTCAGATTA CCAACGACAC TATAATGTAA AAGACGAGCC GGACATGCAG 
TCTGATGAAG GTACAGAGTG GCTCTTGGAG CTGCTCACAG ATGTGCAGCT TCAGCAATAT 
TTCCAGCGTA TCCGTGATGA GCTCAATGTC ACGCGTCTCT CACACTTTGA CTATGTCAAA 
ACAGAGGACC TCGAAAAGAT TGGCATGGGC CGACCAGGTC AAAGGCGGCT GTGGGAGGCA 
GTCAAAAGAA GGAGGGCAAT GTGCAAACGG AAGTCATGGA TGAGCAAGTC TCTGTGTTTG 
GGTCAGCAGG TGTTTACCGG GAAGCGCCCT GACTCCGATT CGCAGACTCC AGAGGTTTTC 
CGCAGCTCGT CCCCTTCATC GGCTCCTCCG GATGGCCAGC CGACGGATCT CACCTGCCTC 
ATCAGCGAAC GAGACCTCAC TCTCTATGAA AAACTGGGGG ACGGATCTTT CGGAGTGGTG 
AAGCGTGGAG AATGGCAGGC CCCCTCTGGA AGAGTGCTAA GCGTGGCTGT GAAGTGCCTG 
AAGACGGACC TGTTGGACCA GGGTGAAGGT CTAGATGACT TCATACGAGA AGTTAATGCC 
ATGCACTCCT TGAACCACCA CAATCTCATT CGTCTGTATG GGTGCGTCCT CACACACCCC 
ATGAAGATGG TGACAGAACT TGCTCCATTG GGTTCTCTGT TGGACCGCCT GAGGAAACGT 
CAGGGTCATA TACTGATATC AGTGCTGTGC CACTACACTG TTCAGATCGC CTGTGGGATG 
GCATACCTGG AGTCTCGACG CTTCATTCAC AGAGACCTGG CTGCACGCAA CATCCTTTTA 
GCCTCCAATG ACCTCATCAA GATCGGTGAC TTCGGCCTGA TGAGGGCCTT GCCTAAAAAT 
GATGATCATT ACGTTATGCA GGAGCATCAC AAAGTCCCTT TTGCATGGTG TGCTCCAGAG 
AGCCTGAAGA CCCGTACCTT CTCCCATGCC AGTGATACCT GGATGTTTGG AGTAACATTG 
TGGGAAATGT TCACTCACGG GCAGGAACCA TGGGTTGGAC TCAATGGTAG TCAGATCCTC 
CATAAGATCG ATAGAGAGGG AGAAAGGCTT ATCAAACCAG AGGACTGTCC TCAGGACATT 
TATAACGTCA TGCTGCAGTG CTGGTCACCC AAACCTGAGG ACAGGCCGAC ATTTGTGTCC 
CTTAGGGACT TCCTTGTGGA GACAATGCCA ACAGATATGA GGGCACTGCA GGACTTTGAT 
GAGCCAGACA AACTTAAAAT CCACGCCAAT GACATCATCA CCATCATTGA GGGCAGAGCA 
GAGAACTACT GGTGGAGGGG TCAGAACAGA CAGACCATGA AGGTGGGACA GTTCCCCAGG 
AATGTAGTGA CCTCTGTGGC CGGTCTATCT GCTCAGGACA TCAGCCGACC TCTCAAGCAC 
AGTTTCATCC ACACAGGCCA TGGAGACACT GACCCACACC GTAGCTGGGG TGCCCCTGAT 
AAGATTGATG ATCTGTACCT TGGTAATCCC ATGGATCCTC CAGATGTTCT TGGGTTGGAT 
TCAAATACAG CCAAGCCCAC TAAGCTACCA AACCGGGCTA AAAAACAACC TCCTCCTAGA 
CCTCCTAAAC CAGCTGTTCT TCTTAAGAAA CCATTTTATG ACTCTGTCCT GGATGATGAT 
GATGATGATG ATCTGATAGT GACAGGTGTG AAGAAGCTCT CACTTAAGAC TCCTACATAC 
CTGGGTTTAC GCCTTCGCCC ATGGGAAAGC ACTAGTCCGA GTGACCGCCT TTGTGAAGTC 
TCCCTTATTG ACTTTGGGGA TGATAGTTTC AGCTCAACAT CGCCCTCTCC CATTACCGAG 
ATTCCCAATC CTAGCACAGC GAAACCACCT TTGTCCTATG CCGCTTCCAT CTTGGATATG 
GCACCACCCC AGAGCCCAAA TCAAGCCTTA CCCAACCCCA TGCACCCTAC TCCTGTGGTA 
GACTGGGACA TGCGGCCCCT GCCTCCTCTC CCGGCATATG ATGAGGTGGC CATTGAGTGT 
GCAGAGCAGG AAGACATAGA AGTGAGCTCC ATAAATGCAT CTGCAGCCCA GGAAGACGCT 
GTATATGAAC AGACCCCATC AGAAGAAGAC ATTAATAATG ATGGTAAACG GAACGTGGAG 
GATAACCTCT TTCTTCCATC AAAGCGTCCT GAGGAATCCC ACTCGTTTTC ACAATCAGCA 
GATATCTTCA AGGAACTTCA AAGAGAGGTG ATGGTAAAGC TTCGGGTCCC TCCAAGTGGG 
CGTTCTCTAC CTTCTTCACC CCTCCCGACT CCTTCAGTTC TCACCCCACA CCGTCAGATA 
ATCCTTCCAT CCTCTTATGA GGACAAGCCT CAGATCCCTC CAAGAATCCC TATTCCACCT 
ATGCGGCCTT CAAAGAGGAC AGGAATATAT GGCAGCAGAC TGTCTGTTTC TCTTGGAGAA 
AATGAAGATG GGAGCCGTTG TCCACCCCAA ATACCCCCGC GGGATCATGC TCTATCTCAG 
CCCAATTCTC GGGCACCAAG TCCTATGGTG CCTCAAAGCG GCTCTCCTCA ACAAAGATCC 
AGCCTGTGCT GTGTTGGATC ATTGGGCTCC TGCTTGTCTC CATCATCATA TTCTTCTGCA 
CCATCCAGCT CGTCCACCAC ATCTTCCACA TCCACCACTA CTAGTTCTCA ATATGGATCT 
ACTGAAGTGA GCCAAAATCC AGCCAAGAGT CCTTGCATCC TGCCAATTGT TCACGGTGGT 
GTAAAGGCCA GCAGCACTCA TTACTACTTG CTACCTGAGA AACCGGCATA CTTGGACAAG 
TATGAGAAGT TCTTTAATTC GGAGGGAAAC GATGAGAAAA GGACTACAAA TATGGCTACT 
GTGAGACCCA TGGTTCAGCA GCAAGGCAAC AAATCCAATG CTTCCCCTAG CCACACCAGT 
AACAGTGGGA CCTCCAGCAG TCTGGCTTGG CCAAGCAGCT CCCAAGCAGG TTCTTACAGC 
CGTGTCACGC TGCAACAAGT ACAGGAAGCA GTACATGGGG TTACTGTAGA GGAGTGTCAA 
ACAGCCTTGC AGGCACACAA CTGGAATGCT CAGGAGGCCG TGAAGTATCT AAAGGTAGAA 
CAGTTATTCC GACTGGGTTT GAAGACTCGG CCTGAATGCA TGGAAACTCT GCAGAGATGT 
GGCTGGAATC TGGAACAGGC CAGCACCCAA ATGCTGGATT CCTATGGTCC ATCCAGGCAT 
AGGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100148041 ( XM_009297484.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295759.2 Danio rerio activated CDC42 kinase 1-like (LOC100148041), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa146351
Clone ID Related Accession (Same CDS sequence)	XM_009297484.3
Accession Version	XM_009297484.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	activated CDC42 kinase 1-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395177.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297484.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC TCTCAGATTA CCAACGACAC TATAATGTAA AAGACGAGCC GGACATGCAG 
TCTGATGAAG GTACAGAGTG GCTCTTGGAG CTGCTCACAG ATGTGCAGCT TCAGCAATAT 
TTCCAGCGTA TCCGTGATGA GCTCAATGTC ACGCGTCTCT CACACTTTGA CTATGTCAAA 
ACAGAGGACC TCGAAAAGAT TGGCATGGGC CGACCAGGTC AAAGGCGGCT GTGGGAGGCA 
GTCAAAAGAA GGAGGGCAAT GTGCAAACGG AAGTCATGGA TGAGCAAGGT GTTTACCGGG 
AAGCGCCCTG ACTCCGATTC GCAGACTCCA GAGGTTTTCC GCAGCTCGTC CCCTTCATCG 
GCTCCTCCGG ATGGCCAGCC GACGGATCTC ACCTGCCTCA TCAGCGAACG AGACCTCACT 
CTCTATGAAA AACTGGGGGA CGGATCTTTC GGAGTGGTGA AGCGTGGAGA ATGGCAGGCC 
CCCTCTGGAA GAGTGCTAAG CGTGGCTGTG AAGTGCCTGA AGACGGACCT GTTGGACCAG 
GGTGAAGGTC TAGATGACTT CATACGAGAA GTTAATGCCA TGCACTCCTT GAACCACCAC 
AATCTCATTC GTCTGTATGG GTGCGTCCTC ACACACCCCA TGAAGATGGT GACAGAACTT 
GCTCCATTGG GTTCTCTGTT GGACCGCCTG AGGAAACGTC AGGGTCATAT ACTGATATCA 
GTGCTGTGCC ACTACACTGT TCAGATCGCC TGTGGGATGG CATACCTGGA GTCTCGACGC 
TTCATTCACA GAGACCTGGC TGCACGCAAC ATCCTTTTAG CCTCCAATGA CCTCATCAAG 
ATCGGTGACT TCGGCCTGAT GAGGGCCTTG CCTAAAAATG ATGATCATTA CGTTATGCAG 
GAGCATCACA AAGTCCCTTT TGCATGGTGT GCTCCAGAGA GCCTGAAGAC CCGTACCTTC 
TCCCATGCCA GTGATACCTG GATGTTTGGA GTAACATTGT GGGAAATGTT CACTCACGGG 
CAGGAACCAT GGGTTGGACT CAATGGTAGT CAGATCCTCC ATAAGATCGA TAGAGAGGGA 
GAAAGGCTTA TCAAACCAGA GGACTGTCCT CAGGACATTT ATAACGTCAT GCTGCAGTGC 
TGGTCACCCA AACCTGAGGA CAGGCCGACA TTTGTGTCCC TTAGGGACTT CCTTGTGGAG 
ACAATGCCAA CAGATATGAG GGCACTGCAG GACTTTGATG AGCCAGACAA ACTTAAAATC 
CACGCCAATG ACATCATCAC CATCATTGAG GGCAGAGCAG AGAACTACTG GTGGAGGGGT 
CAGAACAGAC AGACCATGAA GGTGGGACAG TTCCCCAGGA ATGTAGTGAC CTCTGTGGCC 
GGTCTATCTG CTCAGGACAT CAGCCGACCT CTCAAGCACA GTTTCATCCA CACAGGCCAT 
GGAGACACTG ACCCACACCG TAGCTGGGGT GCCCCTGATA AGATTGATGA TCTGTACCTT 
GGTAATCCCA TGGATCCTCC AGATGTTCTT GGGTTGGATT CAAATACAGC CAAGCCCACT 
AAGCTACCAA ACCGGGCTAA AAAACAACCT CCTCCTAGAC CTCCTAAACC AGCTGTTCTT 
CTTAAGAAAC CATTTTATGA CTCTGTCCTG GATGATGATG ATGATGATGA TCTGATAGTG 
ACAGGTGTGA AGAAGCTCTC ACTTAAGACT CCTACATACC TGGGTTTACG CCTTCGCCCA 
TGGGAAAGCA CTAGTCCGAG TGACCGCCTT TGTGAAGTCT CCCTTATTGA CTTTGGGGAT 
GATAGTTTCA GCTCAACATC GCCCTCTCCC ATTACCGAGA TTCCCAATCC TAGCACAGCG 
AAACCACCTT TGTCCTATGC CGCTTCCATC TTGGATATGG CACCACCCCA GAGCCCAAAT 
CAAGCCTTAC CCAACCCCAT GCACCCTACT CCTGTGGTAG ACTGGGACAT GCGGCCCCTG 
CCTCCTCTCC CGGCATATGA TGAGGTGGCC ATTGAGTGTG CAGAGCAGGA AGACATAGAA 
GTGAGCTCCA TAAATGCATC TGCAGCCCAG GAAGACGCTG TATATGAACA GACCCCATCA 
GAAGAAGACA TTAATAATGA TGGTAAACGG AACGTGGAGG ATAACCTCTT TCTTCCATCA 
AAGCGTCCTG AGGAATCCCA CTCGTTTTCA CAATCAGCAG ATATCTTCAA GGAACTTCAA 
AGAGAGGTGA TGGTAAAGCT TCGGGTCCCT CCAAGTGGGC GTTCTCTACC TTCTTCACCC 
CTCCCGACTC CTTCAGTTCT CACCCCACAC CGTCAGATAA TCCTTCCATC CTCTTATGAG 
GACAAGCCTC AGATCCCTCC AAGAATCCCT ATTCCACCTA TGCGGCCTTC AAAGAGGACA 
GGAATATATG GCAGCAGACT GTCTGTTTCT CTTGGAGAAA ATGAAGATGG GAGCCGTTGT 
CCACCCCAAA TACCCCCGCG GGATCATGCT CTATCTCAGC CCAATTCTCG GGCACCAAGT 
CCTATGGTGC CTCAAAGCGG CTCTCCTCAA CAAAGATCCA GCCTGTGCTG TGTTGGATCA 
TTGGGCTCCT GCTTGTCTCC ATCATCATAT TCTTCTGCAC CATCCAGCTC GTCCACCACA 
TCTTCCACAT CCACCACTAC TAGTTCTCAA TATGGATCTA CTGAAGTGAG CCAAAATCCA 
GCCAAGAGTC CTTGCATCCT GCCAATTGTT CACGGTGGTG TAAAGGCCAG CAGCACTCAT 
TACTACTTGC TACCTGAGAA ACCGGCATAC TTGGACAAGT ATGAGAAGTT CTTTAATTCG 
GAGGGAAACG ATGAGAAAAG GACTACAAAT ATGGCTACTG TGAGACCCAT GGTTCAGCAG 
CAAGGCAACA AATCCAATGC TTCCCCTAGC CACACCAGTA ACAGTGGGAC CTCCAGCAGT 
CTGGCTTGGC CAAGCAGCTC CCAAGCAGGT TCTTACAGCC GTGTCACGCT GCAACAAGTA 
CAGGAAGCAG TACATGGGGT TACTGTAGAG GAGTGTCAAA CAGCCTTGCA GGCACACAAC 
TGGAATGCTC AGGAGGCCGT GAAGTATCTA AAGGTAGAAC AGTTATTCCG ACTGGGTTTG 
AAGACTCGGC CTGAATGCAT GGAAACTCTG CAGAGATGTG GCTGGAATCT GGAACAGGCC 
AGCACCCAAA TGCTGGATTC CTATGGTCCA TCCAGGCATA GGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295759.2
CDS	624..3851
Translation	MAALSDYQRHYNVKDEPDMQSDEGTEWLLELLTDVQLQQYFQRIRDELNVTRLSHFDYVKTEDLEKIGMGRPGQRRLWEAVKRRRAMCKRKSWMSKVFTGKRPDSDSQTPEVFRSSSPSSAPPDGQPTDLTCLISERDLTLYEKLGDGSFGVVKRGEWQAPSGRVLSVAVKCLKTDLLDQGEGLDDFIREVNAMHSLNHHNLIRLYGCVLTHPMKMVTELAPLGSLLDRLRKRQGHILISVLCHYTVQIACGMAYLESRRFIHRDLAARNILLASNDLIKIGDFGLMRALPKNDDHYVMQEHHKVPFAWCAPESLKTRTFSHASDTWMFGVTLWEMFTHGQEPWVGLNGSQILHKIDREGERLIKPEDCPQDIYNVMLQCWSPKPEDRPTFVSLRDFLVETMPTDMRALQDFDEPDKLKIHANDIITIIEGRAENYWWRGQNRQTMKVGQFPRNVVTSVAGLSAQDISRPLKHSFIHTGHGDTDPHRSWGAPDKIDDLYLGNPMDPPDVLGLDSNTAKPTKLPNRAKKQPPPRPPKPAVLLKKPFYDSVLDDDDDDDLIVTGVKKLSLKTPTYLGLRLRPWESTSPSDRLCEVSLIDFGDDSFSSTSPSPITEIPNPSTAKPPLSYAASILDMAPPQSPNQALPNPMHPTPVVDWDMRPLPPLPAYDEVAIECAEQEDIEVSSINASAAQEDAVYEQTPSEEDINNDGKRNVEDNLFLPSKRPEESHSFSQSADIFKELQREVMVKLRVPPSGRSLPSSPLPTPSVLTPHRQIILPSSYEDKPQIPPRIPIPPMRPSKRTGIYGSRLSVSLGENEDGSRCPPQIPPRDHALSQPNSRAPSPMVPQSGSPQQRSSLCCVGSLGSCLSPSSYSSAPSSSSTTSSTSTTTSSQYGSTEVSQNPAKSPCILPIVHGGVKASSTHYYLLPEKPAYLDKYEKFFNSEGNDEKRTTNMATVRPMVQQQGNKSNASPSHTSNSGTSSSLAWPSSSQAGSYSRVTLQQVQEAVHGVTVEECQTALQAHNWNAQEAVKYLKVEQLFRLGLKTRPECMETLQRCGWNLEQASTQMLDSYGPSRHRF

Target ORF information:

RefSeq Version	XM_009297484.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio activated CDC42 kinase 1-like (LOC100148041), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297484.3

ORF Insert Sequence:

ATGGCGGCGC TCTCAGATTA CCAACGACAC TATAATGTAA AAGACGAGCC GGACATGCAG 
TCTGATGAAG GTACAGAGTG GCTCTTGGAG CTGCTCACAG ATGTGCAGCT TCAGCAATAT 
TTCCAGCGTA TCCGTGATGA GCTCAATGTC ACGCGTCTCT CACACTTTGA CTATGTCAAA 
ACAGAGGACC TCGAAAAGAT TGGCATGGGC CGACCAGGTC AAAGGCGGCT GTGGGAGGCA 
GTCAAAAGAA GGAGGGCAAT GTGCAAACGG AAGTCATGGA TGAGCAAGGT GTTTACCGGG 
AAGCGCCCTG ACTCCGATTC GCAGACTCCA GAGGTTTTCC GCAGCTCGTC CCCTTCATCG 
GCTCCTCCGG ATGGCCAGCC GACGGATCTC ACCTGCCTCA TCAGCGAACG AGACCTCACT 
CTCTATGAAA AACTGGGGGA CGGATCTTTC GGAGTGGTGA AGCGTGGAGA ATGGCAGGCC 
CCCTCTGGAA GAGTGCTAAG CGTGGCTGTG AAGTGCCTGA AGACGGACCT GTTGGACCAG 
GGTGAAGGTC TAGATGACTT CATACGAGAA GTTAATGCCA TGCACTCCTT GAACCACCAC 
AATCTCATTC GTCTGTATGG GTGCGTCCTC ACACACCCCA TGAAGATGGT GACAGAACTT 
GCTCCATTGG GTTCTCTGTT GGACCGCCTG AGGAAACGTC AGGGTCATAT ACTGATATCA 
GTGCTGTGCC ACTACACTGT TCAGATCGCC TGTGGGATGG CATACCTGGA GTCTCGACGC 
TTCATTCACA GAGACCTGGC TGCACGCAAC ATCCTTTTAG CCTCCAATGA CCTCATCAAG 
ATCGGTGACT TCGGCCTGAT GAGGGCCTTG CCTAAAAATG ATGATCATTA CGTTATGCAG 
GAGCATCACA AAGTCCCTTT TGCATGGTGT GCTCCAGAGA GCCTGAAGAC CCGTACCTTC 
TCCCATGCCA GTGATACCTG GATGTTTGGA GTAACATTGT GGGAAATGTT CACTCACGGG 
CAGGAACCAT GGGTTGGACT CAATGGTAGT CAGATCCTCC ATAAGATCGA TAGAGAGGGA 
GAAAGGCTTA TCAAACCAGA GGACTGTCCT CAGGACATTT ATAACGTCAT GCTGCAGTGC 
TGGTCACCCA AACCTGAGGA CAGGCCGACA TTTGTGTCCC TTAGGGACTT CCTTGTGGAG 
ACAATGCCAA CAGATATGAG GGCACTGCAG GACTTTGATG AGCCAGACAA ACTTAAAATC 
CACGCCAATG ACATCATCAC CATCATTGAG GGCAGAGCAG AGAACTACTG GTGGAGGGGT 
CAGAACAGAC AGACCATGAA GGTGGGACAG TTCCCCAGGA ATGTAGTGAC CTCTGTGGCC 
GGTCTATCTG CTCAGGACAT CAGCCGACCT CTCAAGCACA GTTTCATCCA CACAGGCCAT 
GGAGACACTG ACCCACACCG TAGCTGGGGT GCCCCTGATA AGATTGATGA TCTGTACCTT 
GGTAATCCCA TGGATCCTCC AGATGTTCTT GGGTTGGATT CAAATACAGC CAAGCCCACT 
AAGCTACCAA ACCGGGCTAA AAAACAACCT CCTCCTAGAC CTCCTAAACC AGCTGTTCTT 
CTTAAGAAAC CATTTTATGA CTCTGTCCTG GATGATGATG ATGATGATGA TCTGATAGTG 
ACAGGTGTGA AGAAGCTCTC ACTTAAGACT CCTACATACC TGGGTTTACG CCTTCGCCCA 
TGGGAAAGCA CTAGTCCGAG TGACCGCCTT TGTGAAGTCT CCCTTATTGA CTTTGGGGAT 
GATAGTTTCA GCTCAACATC GCCCTCTCCC ATTACCGAGA TTCCCAATCC TAGCACAGCG 
AAACCACCTT TGTCCTATGC CGCTTCCATC TTGGATATGG CACCACCCCA GAGCCCAAAT 
CAAGCCTTAC CCAACCCCAT GCACCCTACT CCTGTGGTAG ACTGGGACAT GCGGCCCCTG 
CCTCCTCTCC CGGCATATGA TGAGGTGGCC ATTGAGTGTG CAGAGCAGGA AGACATAGAA 
GTGAGCTCCA TAAATGCATC TGCAGCCCAG GAAGACGCTG TATATGAACA GACCCCATCA 
GAAGAAGACA TTAATAATGA TGGTAAACGG AACGTGGAGG ATAACCTCTT TCTTCCATCA 
AAGCGTCCTG AGGAATCCCA CTCGTTTTCA CAATCAGCAG ATATCTTCAA GGAACTTCAA 
AGAGAGGTGA TGGTAAAGCT TCGGGTCCCT CCAAGTGGGC GTTCTCTACC TTCTTCACCC 
CTCCCGACTC CTTCAGTTCT CACCCCACAC CGTCAGATAA TCCTTCCATC CTCTTATGAG 
GACAAGCCTC AGATCCCTCC AAGAATCCCT ATTCCACCTA TGCGGCCTTC AAAGAGGACA 
GGAATATATG GCAGCAGACT GTCTGTTTCT CTTGGAGAAA ATGAAGATGG GAGCCGTTGT 
CCACCCCAAA TACCCCCGCG GGATCATGCT CTATCTCAGC CCAATTCTCG GGCACCAAGT 
CCTATGGTGC CTCAAAGCGG CTCTCCTCAA CAAAGATCCA GCCTGTGCTG TGTTGGATCA 
TTGGGCTCCT GCTTGTCTCC ATCATCATAT TCTTCTGCAC CATCCAGCTC GTCCACCACA 
TCTTCCACAT CCACCACTAC TAGTTCTCAA TATGGATCTA CTGAAGTGAG CCAAAATCCA 
GCCAAGAGTC CTTGCATCCT GCCAATTGTT CACGGTGGTG TAAAGGCCAG CAGCACTCAT 
TACTACTTGC TACCTGAGAA ACCGGCATAC TTGGACAAGT ATGAGAAGTT CTTTAATTCG 
GAGGGAAACG ATGAGAAAAG GACTACAAAT ATGGCTACTG TGAGACCCAT GGTTCAGCAG 
CAAGGCAACA AATCCAATGC TTCCCCTAGC CACACCAGTA ACAGTGGGAC CTCCAGCAGT 
CTGGCTTGGC CAAGCAGCTC CCAAGCAGGT TCTTACAGCC GTGTCACGCT GCAACAAGTA 
CAGGAAGCAG TACATGGGGT TACTGTAGAG GAGTGTCAAA CAGCCTTGCA GGCACACAAC 
TGGAATGCTC AGGAGGCCGT GAAGTATCTA AAGGTAGAAC AGTTATTCCG ACTGGGTTTG 
AAGACTCGGC CTGAATGCAT GGAAACTCTG CAGAGATGTG GCTGGAATCT GGAACAGGCC 
AGCACCCAAA TGCTGGATTC CTATGGTCCA TCCAGGCATA GGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100148041 ( XM_009297485.3 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295760.2 Danio rerio activated CDC42 kinase 1-like (LOC100148041), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa146352
Clone ID Related Accession (Same CDS sequence)	XM_009297485.3
Accession Version	XM_009297485.3 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3195bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	activated CDC42 kinase 1-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_018395177.1) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_009297485.2. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCTG ATGAAGGTAC AGAGTGGCTC TTGGAGCTGC TCACAGATGT GCAGCTTCAG 
CAATATTTCC AGCGTATCCG TGATGAGCTC AATGTCACGC GTCTCTCACA CTTTGACTAT 
GTCAAAACAG AGGACCTCGA AAAGATTGGC ATGGGCCGAC CAGGTCAAAG GCGGCTGTGG 
GAGGCAGTCA AAAGAAGGAG GGCAATGTGC AAACGGAAGT CATGGATGAG CAAGTCTCTG 
TGTTTGGGTC AGCAGGTGTT TACCGGGAAG CGCCCTGACT CCGATTCGCA GACTCCAGAG 
GTTTTCCGCA GCTCGTCCCC TTCATCGGCT CCTCCGGATG GCCAGCCGAC GGATCTCACC 
TGCCTCATCA GCGAACGAGA CCTCACTCTC TATGAAAAAC TGGGGGACGG ATCTTTCGGA 
GTGGTGAAGC GTGGAGAATG GCAGGCCCCC TCTGGAAGAG TGCTAAGCGT GGCTGTGAAG 
TGCCTGAAGA CGGACCTGTT GGACCAGGGT GAAGGTCTAG ATGACTTCAT ACGAGAAGTT 
AATGCCATGC ACTCCTTGAA CCACCACAAT CTCATTCGTC TGTATGGGTG CGTCCTCACA 
CACCCCATGA AGATGGTGAC AGAACTTGCT CCATTGGGTT CTCTGTTGGA CCGCCTGAGG 
AAACGTCAGG GTCATATACT GATATCAGTG CTGTGCCACT ACACTGTTCA GATCGCCTGT 
GGGATGGCAT ACCTGGAGTC TCGACGCTTC ATTCACAGAG ACCTGGCTGC ACGCAACATC 
CTTTTAGCCT CCAATGACCT CATCAAGATC GGTGACTTCG GCCTGATGAG GGCCTTGCCT 
AAAAATGATG ATCATTACGT TATGCAGGAG CATCACAAAG TCCCTTTTGC ATGGTGTGCT 
CCAGAGAGCC TGAAGACCCG TACCTTCTCC CATGCCAGTG ATACCTGGAT GTTTGGAGTA 
ACATTGTGGG AAATGTTCAC TCACGGGCAG GAACCATGGG TTGGACTCAA TGGTAGTCAG 
ATCCTCCATA AGATCGATAG AGAGGGAGAA AGGCTTATCA AACCAGAGGA CTGTCCTCAG 
GACATTTATA ACGTCATGCT GCAGTGCTGG TCACCCAAAC CTGAGGACAG GCCGACATTT 
GTGTCCCTTA GGGACTTCCT TGTGGAGACA ATGCCAACAG ATATGAGGGC ACTGCAGGAC 
TTTGATGAGC CAGACAAACT TAAAATCCAC GCCAATGACA TCATCACCAT CATTGAGGGC 
AGAGCAGAGA ACTACTGGTG GAGGGGTCAG AACAGACAGA CCATGAAGGT GGGACAGTTC 
CCCAGGAATG TAGTGACCTC TGTGGCCGGT CTATCTGCTC AGGACATCAG CCGACCTCTC 
AAGCACAGTT TCATCCACAC AGGCCATGGA GACACTGACC CACACCGTAG CTGGGGTGCC 
CCTGATAAGA TTGATGATCT GTACCTTGGT AATCCCATGG ATCCTCCAGA TGTTCTTGGG 
TTGGATTCAA ATACAGCCAA GCCCACTAAG CTACCAAACC GGGCTAAAAA ACAACCTCCT 
CCTAGACCTC CTAAACCAGC TGTTCTTCTT AAGAAACCAT TTTATGACTC TGTCCTGGAT 
GATGATGATG ATGATGATCT GATAGTGACA GGTGTGAAGA AGCTCTCACT TAAGACTCCT 
ACATACCTGG GTTTACGCCT TCGCCCATGG GAAAGCACTA GTCCGAGTGA CCGCCTTTGT 
GAAGTCTCCC TTATTGACTT TGGGGATGAT AGTTTCAGCT CAACATCGCC CTCTCCCATT 
ACCGAGATTC CCAATCCTAG CACAGCGAAA CCACCTTTGT CCTATGCCGC TTCCATCTTG 
GATATGGCAC CACCCCAGAG CCCAAATCAA GCCTTACCCA ACCCCATGCA CCCTACTCCT 
GTGGTAGACT GGGACATGCG GCCCCTGCCT CCTCTCCCGG CATATGATGA GGTGGCCATT 
GAGTGTGCAG AGCAGGAAGA CATAGAAGTG AGCTCCATAA ATGCATCTGC AGCCCAGGAA 
GACGCTGTAT ATGAACAGAC CCCATCAGAA GAAGACATTA ATAATGATGG TAAACGGAAC 
GTGGAGGATA ACCTCTTTCT TCCATCAAAG CGTCCTGAGG AATCCCACTC GTTTTCACAA 
TCAGCAGATA TCTTCAAGGA ACTTCAAAGA GAGGTGATGG TAAAGCTTCG GGTCCCTCCA 
AGTGGGCGTT CTCTACCTTC TTCACCCCTC CCGACTCCTT CAGTTCTCAC CCCACACCGT 
CAGATAATCC TTCCATCCTC TTATGAGGAC AAGCCTCAGA TCCCTCCAAG AATCCCTATT 
CCACCTATGC GGCCTTCAAA GAGGACAGGA ATATATGGCA GCAGACTGTC TGTTTCTCTT 
GGAGAAAATG AAGATGGGAG CCGTTGTCCA CCCCAAATAC CCCCGCGGGA TCATGCTCTA 
TCTCAGCCCA ATTCTCGGGC ACCAAGTCCT ATGGTGCCTC AAAGCGGCTC TCCTCAACAA 
AGATCCAGCC TGTGCTGTGT TGGATCATTG GGCTCCTGCT TGTCTCCATC ATCATATTCT 
TCTGCACCAT CCAGCTCGTC CACCACATCT TCCACATCCA CCACTACTAG TTCTCAATAT 
GGATCTACTG AAGTGAGCCA AAATCCAGCC AAGAGTCCTT GCATCCTGCC AATTGTTCAC 
GGTGGTGTAA AGGCCAGCAG CACTCATTAC TACTTGCTAC CTGAGAAACC GGCATACTTG 
GACAAGTATG AGAAGTTCTT TAATTCGGAG GGAAACGATG AGAAAAGGAC TACAAATATG 
GCTACTGTGA GACCCATGGT TCAGCAGCAA GGCAACAAAT CCAATGCTTC CCCTAGCCAC 
ACCAGTAACA GTGGGACCTC CAGCAGTCTG GCTTGGCCAA GCAGCTCCCA AGCAGGTTCT 
TACAGCCGTG TCACGCTGCA ACAAGTACAG GAAGCAGTAC ATGGGGTTAC TGTAGAGGAG 
TGTCAAACAG CCTTGCAGGC ACACAACTGG AATGCTCAGG AGGCCGTGAA GTATCTAAAG 
GTAGAACAGT TATTCCGACT GGGTTTGAAG ACTCGGCCTG AATGCATGGA AACTCTGCAG 
AGATGTGGCT GGAATCTGGA ACAGGCCAGC ACCCAAATGC TGGATTCCTA TGGTCCATCC 
AGGCATAGGT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295760.2
CDS	254..3448
Translation	MQSDEGTEWLLELLTDVQLQQYFQRIRDELNVTRLSHFDYVKTEDLEKIGMGRPGQRRLWEAVKRRRAMCKRKSWMSKSLCLGQQVFTGKRPDSDSQTPEVFRSSSPSSAPPDGQPTDLTCLISERDLTLYEKLGDGSFGVVKRGEWQAPSGRVLSVAVKCLKTDLLDQGEGLDDFIREVNAMHSLNHHNLIRLYGCVLTHPMKMVTELAPLGSLLDRLRKRQGHILISVLCHYTVQIACGMAYLESRRFIHRDLAARNILLASNDLIKIGDFGLMRALPKNDDHYVMQEHHKVPFAWCAPESLKTRTFSHASDTWMFGVTLWEMFTHGQEPWVGLNGSQILHKIDREGERLIKPEDCPQDIYNVMLQCWSPKPEDRPTFVSLRDFLVETMPTDMRALQDFDEPDKLKIHANDIITIIEGRAENYWWRGQNRQTMKVGQFPRNVVTSVAGLSAQDISRPLKHSFIHTGHGDTDPHRSWGAPDKIDDLYLGNPMDPPDVLGLDSNTAKPTKLPNRAKKQPPPRPPKPAVLLKKPFYDSVLDDDDDDDLIVTGVKKLSLKTPTYLGLRLRPWESTSPSDRLCEVSLIDFGDDSFSSTSPSPITEIPNPSTAKPPLSYAASILDMAPPQSPNQALPNPMHPTPVVDWDMRPLPPLPAYDEVAIECAEQEDIEVSSINASAAQEDAVYEQTPSEEDINNDGKRNVEDNLFLPSKRPEESHSFSQSADIFKELQREVMVKLRVPPSGRSLPSSPLPTPSVLTPHRQIILPSSYEDKPQIPPRIPIPPMRPSKRTGIYGSRLSVSLGENEDGSRCPPQIPPRDHALSQPNSRAPSPMVPQSGSPQQRSSLCCVGSLGSCLSPSSYSSAPSSSSTTSSTSTTTSSQYGSTEVSQNPAKSPCILPIVHGGVKASSTHYYLLPEKPAYLDKYEKFFNSEGNDEKRTTNMATVRPMVQQQGNKSNASPSHTSNSGTSSSLAWPSSSQAGSYSRVTLQQVQEAVHGVTVEECQTALQAHNWNAQEAVKYLKVEQLFRLGLKTRPECMETLQRCGWNLEQASTQMLDSYGPSRHRF

Target ORF information:

RefSeq Version	XM_009297485.3
Organism	Danio rerio(zebrafish)
Definition	Danio rerio activated CDC42 kinase 1-like (LOC100148041), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297485.3

ORF Insert Sequence:

ATGCAGTCTG ATGAAGGTAC AGAGTGGCTC TTGGAGCTGC TCACAGATGT GCAGCTTCAG 
CAATATTTCC AGCGTATCCG TGATGAGCTC AATGTCACGC GTCTCTCACA CTTTGACTAT 
GTCAAAACAG AGGACCTCGA AAAGATTGGC ATGGGCCGAC CAGGTCAAAG GCGGCTGTGG 
GAGGCAGTCA AAAGAAGGAG GGCAATGTGC AAACGGAAGT CATGGATGAG CAAGTCTCTG 
TGTTTGGGTC AGCAGGTGTT TACCGGGAAG CGCCCTGACT CCGATTCGCA GACTCCAGAG 
GTTTTCCGCA GCTCGTCCCC TTCATCGGCT CCTCCGGATG GCCAGCCGAC GGATCTCACC 
TGCCTCATCA GCGAACGAGA CCTCACTCTC TATGAAAAAC TGGGGGACGG ATCTTTCGGA 
GTGGTGAAGC GTGGAGAATG GCAGGCCCCC TCTGGAAGAG TGCTAAGCGT GGCTGTGAAG 
TGCCTGAAGA CGGACCTGTT GGACCAGGGT GAAGGTCTAG ATGACTTCAT ACGAGAAGTT 
AATGCCATGC ACTCCTTGAA CCACCACAAT CTCATTCGTC TGTATGGGTG CGTCCTCACA 
CACCCCATGA AGATGGTGAC AGAACTTGCT CCATTGGGTT CTCTGTTGGA CCGCCTGAGG 
AAACGTCAGG GTCATATACT GATATCAGTG CTGTGCCACT ACACTGTTCA GATCGCCTGT 
GGGATGGCAT ACCTGGAGTC TCGACGCTTC ATTCACAGAG ACCTGGCTGC ACGCAACATC 
CTTTTAGCCT CCAATGACCT CATCAAGATC GGTGACTTCG GCCTGATGAG GGCCTTGCCT 
AAAAATGATG ATCATTACGT TATGCAGGAG CATCACAAAG TCCCTTTTGC ATGGTGTGCT 
CCAGAGAGCC TGAAGACCCG TACCTTCTCC CATGCCAGTG ATACCTGGAT GTTTGGAGTA 
ACATTGTGGG AAATGTTCAC TCACGGGCAG GAACCATGGG TTGGACTCAA TGGTAGTCAG 
ATCCTCCATA AGATCGATAG AGAGGGAGAA AGGCTTATCA AACCAGAGGA CTGTCCTCAG 
GACATTTATA ACGTCATGCT GCAGTGCTGG TCACCCAAAC CTGAGGACAG GCCGACATTT 
GTGTCCCTTA GGGACTTCCT TGTGGAGACA ATGCCAACAG ATATGAGGGC ACTGCAGGAC 
TTTGATGAGC CAGACAAACT TAAAATCCAC GCCAATGACA TCATCACCAT CATTGAGGGC 
AGAGCAGAGA ACTACTGGTG GAGGGGTCAG AACAGACAGA CCATGAAGGT GGGACAGTTC 
CCCAGGAATG TAGTGACCTC TGTGGCCGGT CTATCTGCTC AGGACATCAG CCGACCTCTC 
AAGCACAGTT TCATCCACAC AGGCCATGGA GACACTGACC CACACCGTAG CTGGGGTGCC 
CCTGATAAGA TTGATGATCT GTACCTTGGT AATCCCATGG ATCCTCCAGA TGTTCTTGGG 
TTGGATTCAA ATACAGCCAA GCCCACTAAG CTACCAAACC GGGCTAAAAA ACAACCTCCT 
CCTAGACCTC CTAAACCAGC TGTTCTTCTT AAGAAACCAT TTTATGACTC TGTCCTGGAT 
GATGATGATG ATGATGATCT GATAGTGACA GGTGTGAAGA AGCTCTCACT TAAGACTCCT 
ACATACCTGG GTTTACGCCT TCGCCCATGG GAAAGCACTA GTCCGAGTGA CCGCCTTTGT 
GAAGTCTCCC TTATTGACTT TGGGGATGAT AGTTTCAGCT CAACATCGCC CTCTCCCATT 
ACCGAGATTC CCAATCCTAG CACAGCGAAA CCACCTTTGT CCTATGCCGC TTCCATCTTG 
GATATGGCAC CACCCCAGAG CCCAAATCAA GCCTTACCCA ACCCCATGCA CCCTACTCCT 
GTGGTAGACT GGGACATGCG GCCCCTGCCT CCTCTCCCGG CATATGATGA GGTGGCCATT 
GAGTGTGCAG AGCAGGAAGA CATAGAAGTG AGCTCCATAA ATGCATCTGC AGCCCAGGAA 
GACGCTGTAT ATGAACAGAC CCCATCAGAA GAAGACATTA ATAATGATGG TAAACGGAAC 
GTGGAGGATA ACCTCTTTCT TCCATCAAAG CGTCCTGAGG AATCCCACTC GTTTTCACAA 
TCAGCAGATA TCTTCAAGGA ACTTCAAAGA GAGGTGATGG TAAAGCTTCG GGTCCCTCCA 
AGTGGGCGTT CTCTACCTTC TTCACCCCTC CCGACTCCTT CAGTTCTCAC CCCACACCGT 
CAGATAATCC TTCCATCCTC TTATGAGGAC AAGCCTCAGA TCCCTCCAAG AATCCCTATT 
CCACCTATGC GGCCTTCAAA GAGGACAGGA ATATATGGCA GCAGACTGTC TGTTTCTCTT 
GGAGAAAATG AAGATGGGAG CCGTTGTCCA CCCCAAATAC CCCCGCGGGA TCATGCTCTA 
TCTCAGCCCA ATTCTCGGGC ACCAAGTCCT ATGGTGCCTC AAAGCGGCTC TCCTCAACAA 
AGATCCAGCC TGTGCTGTGT TGGATCATTG GGCTCCTGCT TGTCTCCATC ATCATATTCT 
TCTGCACCAT CCAGCTCGTC CACCACATCT TCCACATCCA CCACTACTAG TTCTCAATAT 
GGATCTACTG AAGTGAGCCA AAATCCAGCC AAGAGTCCTT GCATCCTGCC AATTGTTCAC 
GGTGGTGTAA AGGCCAGCAG CACTCATTAC TACTTGCTAC CTGAGAAACC GGCATACTTG 
GACAAGTATG AGAAGTTCTT TAATTCGGAG GGAAACGATG AGAAAAGGAC TACAAATATG 
GCTACTGTGA GACCCATGGT TCAGCAGCAA GGCAACAAAT CCAATGCTTC CCCTAGCCAC 
ACCAGTAACA GTGGGACCTC CAGCAGTCTG GCTTGGCCAA GCAGCTCCCA AGCAGGTTCT 
TACAGCCGTG TCACGCTGCA ACAAGTACAG GAAGCAGTAC ATGGGGTTAC TGTAGAGGAG 
TGTCAAACAG CCTTGCAGGC ACACAACTGG AATGCTCAGG AGGCCGTGAA GTATCTAAAG 
GTAGAACAGT TATTCCGACT GGGTTTGAAG ACTCGGCCTG AATGCATGGA AACTCTGCAG 
AGATGTGGCT GGAATCTGGA ACAGGCCAGC ACCCAAATGC TGGATTCCTA TGGTCCATCC 
AGGCATAGGT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100148041 ( XM_009297484.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295759.1 Danio rerio activated CDC42 kinase 1-like (LOC100148041), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa28667
Clone ID Related Accession (Same CDS sequence)	XM_009297484.2
Accession Version	XM_009297484.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3228bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	activated CDC42 kinase 1-like isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297484.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC TCTCAGATTA CCAACGACAC TATAATGTAA AAGACGAGCC GGACATGCAG 
TCTGATGAAG GTACAGAGTG GCTCTTGGAG CTGCTCACAG ATGTGCAGCT TCAGCAATAT 
TTCCAGCGTA TCCGTGATGA GCTCAATGTC ACGCGTCTCT CACACTTTGA CTATGTCAAA 
ACAGAGGACC TCGAAAAAAT TGGCATGGGC CGACCAGGTC AAAGGCGGCT GTGGGAGGCA 
GTCAAAAGAA GAAGGGCAAT GTGCAAACGG AAGTCATGGA TGAGCAAGGT GTTTACCGGG 
AAGCGCCCTG ACTCTGATTC GCAGACTCCA GAGGTTTTCC GCAGCTCGTC CCCTTCATCG 
GCTCCTCCGG ATGGCCAGCC GACGGATCTC ACCTGCCTCA TCAGCGAACG AGACCTCACT 
CTCTATGAAA AACTGGGGGA CGGATCTTTC GGAGTGGTGA AGCGTGGAGA ATGGCAGGCC 
CCCTCTGGAA GAGTGCTAAG CGTGGCTGTG AAGTGCCTGA AGACGGACCT GTTGGACCAG 
GGTGAAGGTC TAGATGACTT CATACGAGAA GTTAATGCCA TGCACTCCTT GAACCACCAC 
AATCTCATTC GTCTGTATGG GTGCGTCCTC ACACACCCCA TGAAGATGGT GACAGAACTT 
GCTCCATTGG GTTCTCTGTT GGACCGCCTG AGGAAACGTC AGGGTCATAT ACTGATATCA 
GTGCTGTGCC ACTACACTGT TCAGATCGCC TGTGGGATGG CATACCTGGA GTCTCGACGC 
TTCATTCACA GAGACCTGGC TGCACGCAAC ATCCTTTTAG CCTCCAATGA CCTCATCAAG 
ATCGGTGACT TCGGCCTGAT GAGGGCCTTG CCTAAAAATG ATGATCATTA CGTTATGCAG 
GAGCATCACA AAGTCCCTTT TGCATGGTGT GCTCCAGAGA GCCTGAAGAC CCGTACCTTC 
TCCCATGCCA GTGATACCTG GATGTTTGGA GTAACATTGT GGGAAATGTT CACTCACGGG 
CAGGAACCAT GGGTTGGACT CAATGGTAGT CAGATCCTCC ATAAGATCGA TAGAGAGGGA 
GAAAGGCTTA TCAAACCAGA GGACTGTCCT CAGGACATTT ATAACGTCAT GCTGCAGTGC 
TGGTCACCCA AACCTGAGGA CAGGCCAACA TTTGTGTCCC TTAGGGACTT CCTTGTGGAG 
ACAATGCCAA CAGATATGAG GGCACTGCAG GACTTTGATG AGCCAGACAA ACTTAAAATC 
CACACTAATG ACATCATCAC CATCATTGAG GGCAGAGCAG AGAACTACTG GTGGAGGGGT 
CAGAACAGAC AGACCATGAA GGTGGGACAG TTCCCCAGGA ATGTAGTGAC CTCTGTGGCC 
GGTCTATCTG CTCAGGACAT CAGCCGACCT CTCAAGCACA GTTTCATCCA CACAGGCCAT 
GGAGACACTG ACCCACACCG TAGCTGGGGT GCCCCTGATA AGATTGATGA TCTGTACCTT 
GGTAATCCCA TGGATCCTCC AGATGTTCTT GGGTTGGATT CAAATACAGC CAAGCCCACT 
AAGCTACCAA ACCGGGCTAA AAAACAACCT CCTCCTAGAC CTCCTAAACC AGCTGTTCTT 
CTTAAGAAAC CATTTTATGA CTCTGTCCTG GATGATGATG ATGATGATGA TCTGATAGTG 
ACAGGTGTGA AGAAGCTCTC ACTTAAGACT CCTACATACC TGGGTTTACG CCTTCGCCCA 
TGGGAAAGCA CTAGTCCGAG TGACCGCCTT TGTGAAGTCT CCCTTATTGA CTTTGGGGAT 
GATAGTTTCA GCTCAACATC GCCCTCTCCC ATTACCGAGA TTCCCAATCC TAGCACAGCG 
AAACCACCTT TGTCCTATGC CGCTTCCATC TTGGATATGG CACCACCCCA GAGCCCAAAT 
CAAGCCTTAC CCAACCCCAT GCACCCTACT CCAGTGGTAG ACTGGGACAT GCGGCCCCTG 
CCTCCTCTCC CGGCATATGA TGAGGTGGCC GTTGAGTGTG CAGAGCAGGA AGACATAGAA 
GTGAGCTCCA TAAATGCATC TGCAGCCCAG GAAGACACTG TATATGAACA GACCCCATCA 
GAAGAAGACA TTAATAATGA TGGTAAACGG AACGTGGAGG ATAACCTCTT TCTTCCATCA 
AAGCGTCCTG AGGAATCCCA CTCGTTTTCA CAATCAGCAG ATATCTTCAA GGAACTGCAA 
AGAGAGGTGA TGGTAAAGCT TCGGGTCCCT CCAAGTGGGC GTTCTCTACC TTCTTCACCC 
CTCCCGACTC CTTCAGTTCT CACCCCACAC CGTCAGATAA TCCTTCCATC CTCTTATGAG 
GACAAGCCTC AGATCCCTCC AAGAATCCCT ATTCCACCTA TGCGGCCTTC AAAGAGGACA 
GGAATATACG GCAGCAGACT GTCTGTTTCT CTTGGAGAAA ATGAAGATGG GAGCCGTTGT 
CCACCCCAAA TACCCCCGCG CGATCATGCT CTATCTCAGC CCAATTCTCG GGCACCAAGT 
CCTATGGTGC CTCAAAGCGG CTCTCCTCAA CAAAGATCCA GCCTGTGCTG TGTTGGATCA 
TTGGGCTCCT GCTTGTCTCC ATCATCATAT TCTTCTGCAC CATCCAGCTC GTCCACCACA 
TCTTCCACAT CCACCACTAC TAGTTTTCAA TATGGATCTA CTGAAGTGAG CCAAAATCCA 
GCCAAGAGTC CTTGCATCCT GCCAATTGTT TACGGTGGTG TAAAGGCCAG CAGCACTCAT 
TACTACTTGC TACCTGAGAA ACCGGCATAC TTGGACAAGT ATGAGAAGTT CTTTAATTCG 
GAGGGAAACG ATGAGAAAAG GACTACAAAT ATGGCTACTG TGAGACCCAT GGTTCAGCAG 
CAAGGCAACA AATCCAATGC TTCCCCAAGC CACACCAGTA ACAGTGGGAC CTCCAGCAGT 
CTGGCTTGGC CAAGCAGCTC CCAAGCAGGT TCTTACAGCC GTGTCACGCT GCAACAAGTA 
CAGGAAGCAG TACATGGGGT GACTGTAGAG GAGTGTCAAA CAGCCTTGCA GGCACACAAC 
TGGAATGCTC AGGAGGCCGT GAAGTATCTA AAGGTAGAAC AGTTATTCCG ACTGGGTTTG 
AAGACTCGGC CTGAATGCAT GGAAACTCTG CAGAGATGTG GCTGGAATCT GGAACAGGCC 
AGCACCCAAA TGCTGGATTC CTATGGTCCA TCTAGGCATA GGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295759.1
CDS	619..3846
Translation	MAALSDYQRHYNVKDEPDMQSDEGTEWLLELLTDVQLQQYFQRIRDELNVTRLSHFDYVKTEDLEKIGMGRPGQRRLWEAVKRRRAMCKRKSWMSKVFTGKRPDSDSQTPEVFRSSSPSSAPPDGQPTDLTCLISERDLTLYEKLGDGSFGVVKRGEWQAPSGRVLSVAVKCLKTDLLDQGEGLDDFIREVNAMHSLNHHNLIRLYGCVLTHPMKMVTELAPLGSLLDRLRKRQGHILISVLCHYTVQIACGMAYLESRRFIHRDLAARNILLASNDLIKIGDFGLMRALPKNDDHYVMQEHHKVPFAWCAPESLKTRTFSHASDTWMFGVTLWEMFTHGQEPWVGLNGSQILHKIDREGERLIKPEDCPQDIYNVMLQCWSPKPEDRPTFVSLRDFLVETMPTDMRALQDFDEPDKLKIHTNDIITIIEGRAENYWWRGQNRQTMKVGQFPRNVVTSVAGLSAQDISRPLKHSFIHTGHGDTDPHRSWGAPDKIDDLYLGNPMDPPDVLGLDSNTAKPTKLPNRAKKQPPPRPPKPAVLLKKPFYDSVLDDDDDDDLIVTGVKKLSLKTPTYLGLRLRPWESTSPSDRLCEVSLIDFGDDSFSSTSPSPITEIPNPSTAKPPLSYAASILDMAPPQSPNQALPNPMHPTPVVDWDMRPLPPLPAYDEVAVECAEQEDIEVSSINASAAQEDTVYEQTPSEEDINNDGKRNVEDNLFLPSKRPEESHSFSQSADIFKELQREVMVKLRVPPSGRSLPSSPLPTPSVLTPHRQIILPSSYEDKPQIPPRIPIPPMRPSKRTGIYGSRLSVSLGENEDGSRCPPQIPPRDHALSQPNSRAPSPMVPQSGSPQQRSSLCCVGSLGSCLSPSSYSSAPSSSSTTSSTSTTTSFQYGSTEVSQNPAKSPCILPIVYGGVKASSTHYYLLPEKPAYLDKYEKFFNSEGNDEKRTTNMATVRPMVQQQGNKSNASPSHTSNSGTSSSLAWPSSSQAGSYSRVTLQQVQEAVHGVTVEECQTALQAHNWNAQEAVKYLKVEQLFRLGLKTRPECMETLQRCGWNLEQASTQMLDSYGPSRHRF

Target ORF information:

RefSeq Version	XM_009297484.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio activated CDC42 kinase 1-like (LOC100148041), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297484.2

ORF Insert Sequence:

ATGGCGGCGC TCTCAGATTA CCAACGACAC TATAATGTAA AAGACGAGCC GGACATGCAG 
TCTGATGAAG GTACAGAGTG GCTCTTGGAG CTGCTCACAG ATGTGCAGCT TCAGCAATAT 
TTCCAGCGTA TCCGTGATGA GCTCAATGTC ACGCGTCTCT CACACTTTGA CTATGTCAAA 
ACAGAGGACC TCGAAAAAAT TGGCATGGGC CGACCAGGTC AAAGGCGGCT GTGGGAGGCA 
GTCAAAAGAA GAAGGGCAAT GTGCAAACGG AAGTCATGGA TGAGCAAGGT GTTTACCGGG 
AAGCGCCCTG ACTCTGATTC GCAGACTCCA GAGGTTTTCC GCAGCTCGTC CCCTTCATCG 
GCTCCTCCGG ATGGCCAGCC GACGGATCTC ACCTGCCTCA TCAGCGAACG AGACCTCACT 
CTCTATGAAA AACTGGGGGA CGGATCTTTC GGAGTGGTGA AGCGTGGAGA ATGGCAGGCC 
CCCTCTGGAA GAGTGCTAAG CGTGGCTGTG AAGTGCCTGA AGACGGACCT GTTGGACCAG 
GGTGAAGGTC TAGATGACTT CATACGAGAA GTTAATGCCA TGCACTCCTT GAACCACCAC 
AATCTCATTC GTCTGTATGG GTGCGTCCTC ACACACCCCA TGAAGATGGT GACAGAACTT 
GCTCCATTGG GTTCTCTGTT GGACCGCCTG AGGAAACGTC AGGGTCATAT ACTGATATCA 
GTGCTGTGCC ACTACACTGT TCAGATCGCC TGTGGGATGG CATACCTGGA GTCTCGACGC 
TTCATTCACA GAGACCTGGC TGCACGCAAC ATCCTTTTAG CCTCCAATGA CCTCATCAAG 
ATCGGTGACT TCGGCCTGAT GAGGGCCTTG CCTAAAAATG ATGATCATTA CGTTATGCAG 
GAGCATCACA AAGTCCCTTT TGCATGGTGT GCTCCAGAGA GCCTGAAGAC CCGTACCTTC 
TCCCATGCCA GTGATACCTG GATGTTTGGA GTAACATTGT GGGAAATGTT CACTCACGGG 
CAGGAACCAT GGGTTGGACT CAATGGTAGT CAGATCCTCC ATAAGATCGA TAGAGAGGGA 
GAAAGGCTTA TCAAACCAGA GGACTGTCCT CAGGACATTT ATAACGTCAT GCTGCAGTGC 
TGGTCACCCA AACCTGAGGA CAGGCCAACA TTTGTGTCCC TTAGGGACTT CCTTGTGGAG 
ACAATGCCAA CAGATATGAG GGCACTGCAG GACTTTGATG AGCCAGACAA ACTTAAAATC 
CACACTAATG ACATCATCAC CATCATTGAG GGCAGAGCAG AGAACTACTG GTGGAGGGGT 
CAGAACAGAC AGACCATGAA GGTGGGACAG TTCCCCAGGA ATGTAGTGAC CTCTGTGGCC 
GGTCTATCTG CTCAGGACAT CAGCCGACCT CTCAAGCACA GTTTCATCCA CACAGGCCAT 
GGAGACACTG ACCCACACCG TAGCTGGGGT GCCCCTGATA AGATTGATGA TCTGTACCTT 
GGTAATCCCA TGGATCCTCC AGATGTTCTT GGGTTGGATT CAAATACAGC CAAGCCCACT 
AAGCTACCAA ACCGGGCTAA AAAACAACCT CCTCCTAGAC CTCCTAAACC AGCTGTTCTT 
CTTAAGAAAC CATTTTATGA CTCTGTCCTG GATGATGATG ATGATGATGA TCTGATAGTG 
ACAGGTGTGA AGAAGCTCTC ACTTAAGACT CCTACATACC TGGGTTTACG CCTTCGCCCA 
TGGGAAAGCA CTAGTCCGAG TGACCGCCTT TGTGAAGTCT CCCTTATTGA CTTTGGGGAT 
GATAGTTTCA GCTCAACATC GCCCTCTCCC ATTACCGAGA TTCCCAATCC TAGCACAGCG 
AAACCACCTT TGTCCTATGC CGCTTCCATC TTGGATATGG CACCACCCCA GAGCCCAAAT 
CAAGCCTTAC CCAACCCCAT GCACCCTACT CCAGTGGTAG ACTGGGACAT GCGGCCCCTG 
CCTCCTCTCC CGGCATATGA TGAGGTGGCC GTTGAGTGTG CAGAGCAGGA AGACATAGAA 
GTGAGCTCCA TAAATGCATC TGCAGCCCAG GAAGACACTG TATATGAACA GACCCCATCA 
GAAGAAGACA TTAATAATGA TGGTAAACGG AACGTGGAGG ATAACCTCTT TCTTCCATCA 
AAGCGTCCTG AGGAATCCCA CTCGTTTTCA CAATCAGCAG ATATCTTCAA GGAACTGCAA 
AGAGAGGTGA TGGTAAAGCT TCGGGTCCCT CCAAGTGGGC GTTCTCTACC TTCTTCACCC 
CTCCCGACTC CTTCAGTTCT CACCCCACAC CGTCAGATAA TCCTTCCATC CTCTTATGAG 
GACAAGCCTC AGATCCCTCC AAGAATCCCT ATTCCACCTA TGCGGCCTTC AAAGAGGACA 
GGAATATACG GCAGCAGACT GTCTGTTTCT CTTGGAGAAA ATGAAGATGG GAGCCGTTGT 
CCACCCCAAA TACCCCCGCG CGATCATGCT CTATCTCAGC CCAATTCTCG GGCACCAAGT 
CCTATGGTGC CTCAAAGCGG CTCTCCTCAA CAAAGATCCA GCCTGTGCTG TGTTGGATCA 
TTGGGCTCCT GCTTGTCTCC ATCATCATAT TCTTCTGCAC CATCCAGCTC GTCCACCACA 
TCTTCCACAT CCACCACTAC TAGTTTTCAA TATGGATCTA CTGAAGTGAG CCAAAATCCA 
GCCAAGAGTC CTTGCATCCT GCCAATTGTT TACGGTGGTG TAAAGGCCAG CAGCACTCAT 
TACTACTTGC TACCTGAGAA ACCGGCATAC TTGGACAAGT ATGAGAAGTT CTTTAATTCG 
GAGGGAAACG ATGAGAAAAG GACTACAAAT ATGGCTACTG TGAGACCCAT GGTTCAGCAG 
CAAGGCAACA AATCCAATGC TTCCCCAAGC CACACCAGTA ACAGTGGGAC CTCCAGCAGT 
CTGGCTTGGC CAAGCAGCTC CCAAGCAGGT TCTTACAGCC GTGTCACGCT GCAACAAGTA 
CAGGAAGCAG TACATGGGGT GACTGTAGAG GAGTGTCAAA CAGCCTTGCA GGCACACAAC 
TGGAATGCTC AGGAGGCCGT GAAGTATCTA AAGGTAGAAC AGTTATTCCG ACTGGGTTTG 
AAGACTCGGC CTGAATGCAT GGAAACTCTG CAGAGATGTG GCTGGAATCT GGAACAGGCC 
AGCACCCAAA TGCTGGATTC CTATGGTCCA TCTAGGCATA GGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100148041 ( XM_021470344.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326019.1 Danio rerio activated CDC42 kinase 1-like (LOC100148041), transcript variant X1, mRNA.

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CloneID	ODa48613
Clone ID Related Accession (Same CDS sequence)	XM_021470344.1 , XM_009297483.2
Accession Version	XM_021470344.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	activated CDC42 kinase 1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC TCTCAGATTA CCAACGACAC TATAATGTAA AAGACGAGCC GGACATGCAG 
TCTGATGAAG GTACAGAGTG GCTCTTGGAG CTGCTCACAG ATGTGCAGCT TCAGCAATAT 
TTCCAGCGTA TCCGTGATGA GCTCAATGTC ACGCGTCTCT CACACTTTGA CTATGTCAAA 
ACAGAGGACC TCGAAAAAAT TGGCATGGGC CGACCAGGTC AAAGGCGGCT GTGGGAGGCA 
GTCAAAAGAA GAAGGGCAAT GTGCAAACGG AAGTCATGGA TGAGCAAGTC TCTGTGTTTG 
GGTCAGCAGG TGTTTACCGG GAAGCGCCCT GACTCTGATT CGCAGACTCC AGAGGTTTTC 
CGCAGCTCGT CCCCTTCATC GGCTCCTCCG GATGGCCAGC CGACGGATCT CACCTGCCTC 
ATCAGCGAAC GAGACCTCAC TCTCTATGAA AAACTGGGGG ACGGATCTTT CGGAGTGGTG 
AAGCGTGGAG AATGGCAGGC CCCCTCTGGA AGAGTGCTAA GCGTGGCTGT GAAGTGCCTG 
AAGACGGACC TGTTGGACCA GGGTGAAGGT CTAGATGACT TCATACGAGA AGTTAATGCC 
ATGCACTCCT TGAACCACCA CAATCTCATT CGTCTGTATG GGTGCGTCCT CACACACCCC 
ATGAAGATGG TGACAGAACT TGCTCCATTG GGTTCTCTGT TGGACCGCCT GAGGAAACGT 
CAGGGTCATA TACTGATATC AGTGCTGTGC CACTACACTG TTCAGATCGC CTGTGGGATG 
GCATACCTGG AGTCTCGACG CTTCATTCAC AGAGACCTGG CTGCACGCAA CATCCTTTTA 
GCCTCCAATG ACCTCATCAA GATCGGTGAC TTCGGCCTGA TGAGGGCCTT GCCTAAAAAT 
GATGATCATT ACGTTATGCA GGAGCATCAC AAAGTCCCTT TTGCATGGTG TGCTCCAGAG 
AGCCTGAAGA CCCGTACCTT CTCCCATGCC AGTGATACCT GGATGTTTGG AGTAACATTG 
TGGGAAATGT TCACTCACGG GCAGGAACCA TGGGTTGGAC TCAATGGTAG TCAGATCCTC 
CATAAGATCG ATAGAGAGGG AGAAAGGCTT ATCAAACCAG AGGACTGTCC TCAGGACATT 
TATAACGTCA TGCTGCAGTG CTGGTCACCC AAACCTGAGG ACAGGCCAAC ATTTGTGTCC 
CTTAGGGACT TCCTTGTGGA GACAATGCCA ACAGATATGA GGGCACTGCA GGACTTTGAT 
GAGCCAGACA AACTTAAAAT CCACACTAAT GACATCATCA CCATCATTGA GGGCAGAGCA 
GAGAACTACT GGTGGAGGGG TCAGAACAGA CAGACCATGA AGGTGGGACA GTTCCCCAGG 
AATGTAGTGA CCTCTGTGGC CGGTCTATCT GCTCAGGACA TCAGCCGACC TCTCAAGCAC 
AGTTTCATCC ACACAGGCCA TGGAGACACT GACCCACACC GTAGCTGGGG TGCCCCTGAT 
AAGATTGATG ATCTGTACCT TGGTAATCCC ATGGATCCTC CAGATGTTCT TGGGTTGGAT 
TCAAATACAG CCAAGCCCAC TAAGCTACCA AACCGGGCTA AAAAACAACC TCCTCCTAGA 
CCTCCTAAAC CAGCTGTTCT TCTTAAGAAA CCATTTTATG ACTCTGTCCT GGATGATGAT 
GATGATGATG ATCTGATAGT GACAGGTGTG AAGAAGCTCT CACTTAAGAC TCCTACATAC 
CTGGGTTTAC GCCTTCGCCC ATGGGAAAGC ACTAGTCCGA GTGACCGCCT TTGTGAAGTC 
TCCCTTATTG ACTTTGGGGA TGATAGTTTC AGCTCAACAT CGCCCTCTCC CATTACCGAG 
ATTCCCAATC CTAGCACAGC GAAACCACCT TTGTCCTATG CCGCTTCCAT CTTGGATATG 
GCACCACCCC AGAGCCCAAA TCAAGCCTTA CCCAACCCCA TGCACCCTAC TCCAGTGGTA 
GACTGGGACA TGCGGCCCCT GCCTCCTCTC CCGGCATATG ATGAGGTGGC CGTTGAGTGT 
GCAGAGCAGG AAGACATAGA AGTGAGCTCC ATAAATGCAT CTGCAGCCCA GGAAGACACT 
GTATATGAAC AGACCCCATC AGAAGAAGAC ATTAATAATG ATGGTAAACG GAACGTGGAG 
GATAACCTCT TTCTTCCATC AAAGCGTCCT GAGGAATCCC ACTCGTTTTC ACAATCAGCA 
GATATCTTCA AGGAACTGCA AAGAGAGGTG ATGGTAAAGC TTCGGGTCCC TCCAAGTGGG 
CGTTCTCTAC CTTCTTCACC CCTCCCGACT CCTTCAGTTC TCACCCCACA CCGTCAGATA 
ATCCTTCCAT CCTCTTATGA GGACAAGCCT CAGATCCCTC CAAGAATCCC TATTCCACCT 
ATGCGGCCTT CAAAGAGGAC AGGAATATAC GGCAGCAGAC TGTCTGTTTC TCTTGGAGAA 
AATGAAGATG GGAGCCGTTG TCCACCCCAA ATACCCCCGC GCGATCATGC TCTATCTCAG 
CCCAATTCTC GGGCACCAAG TCCTATGGTG CCTCAAAGCG GCTCTCCTCA ACAAAGATCC 
AGCCTGTGCT GTGTTGGATC ATTGGGCTCC TGCTTGTCTC CATCATCATA TTCTTCTGCA 
CCATCCAGCT CGTCCACCAC ATCTTCCACA TCCACCACTA CTAGTTTTCA ATATGGATCT 
ACTGAAGTGA GCCAAAATCC AGCCAAGAGT CCTTGCATCC TGCCAATTGT TTACGGTGGT 
GTAAAGGCCA GCAGCACTCA TTACTACTTG CTACCTGAGA AACCGGCATA CTTGGACAAG 
TATGAGAAGT TCTTTAATTC GGAGGGAAAC GATGAGAAAA GGACTACAAA TATGGCTACT 
GTGAGACCCA TGGTTCAGCA GCAAGGCAAC AAATCCAATG CTTCCCCAAG CCACACCAGT 
AACAGTGGGA CCTCCAGCAG TCTGGCTTGG CCAAGCAGCT CCCAAGCAGG TTCTTACAGC 
CGTGTCACGC TGCAACAAGT ACAGGAAGCA GTACATGGGG TGACTGTAGA GGAGTGTCAA 
ACAGCCTTGC AGGCACACAA CTGGAATGCT CAGGAGGCCG TGAAGTATCT AAAGGTAGAA 
CAGTTATTCC GACTGGGTTT GAAGACTCGG CCTGAATGCA TGGAAACTCT GCAGAGATGT 
GGCTGGAATC TGGAACAGGC CAGCACCCAA ATGCTGGATT CCTATGGTCC ATCTAGGCAT 
AGGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326019.1
CDS	630..3878
Translation	MAALSDYQRHYNVKDEPDMQSDEGTEWLLELLTDVQLQQYFQRIRDELNVTRLSHFDYVKTEDLEKIGMGRPGQRRLWEAVKRRRAMCKRKSWMSKSLCLGQQVFTGKRPDSDSQTPEVFRSSSPSSAPPDGQPTDLTCLISERDLTLYEKLGDGSFGVVKRGEWQAPSGRVLSVAVKCLKTDLLDQGEGLDDFIREVNAMHSLNHHNLIRLYGCVLTHPMKMVTELAPLGSLLDRLRKRQGHILISVLCHYTVQIACGMAYLESRRFIHRDLAARNILLASNDLIKIGDFGLMRALPKNDDHYVMQEHHKVPFAWCAPESLKTRTFSHASDTWMFGVTLWEMFTHGQEPWVGLNGSQILHKIDREGERLIKPEDCPQDIYNVMLQCWSPKPEDRPTFVSLRDFLVETMPTDMRALQDFDEPDKLKIHTNDIITIIEGRAENYWWRGQNRQTMKVGQFPRNVVTSVAGLSAQDISRPLKHSFIHTGHGDTDPHRSWGAPDKIDDLYLGNPMDPPDVLGLDSNTAKPTKLPNRAKKQPPPRPPKPAVLLKKPFYDSVLDDDDDDDLIVTGVKKLSLKTPTYLGLRLRPWESTSPSDRLCEVSLIDFGDDSFSSTSPSPITEIPNPSTAKPPLSYAASILDMAPPQSPNQALPNPMHPTPVVDWDMRPLPPLPAYDEVAVECAEQEDIEVSSINASAAQEDTVYEQTPSEEDINNDGKRNVEDNLFLPSKRPEESHSFSQSADIFKELQREVMVKLRVPPSGRSLPSSPLPTPSVLTPHRQIILPSSYEDKPQIPPRIPIPPMRPSKRTGIYGSRLSVSLGENEDGSRCPPQIPPRDHALSQPNSRAPSPMVPQSGSPQQRSSLCCVGSLGSCLSPSSYSSAPSSSSTTSSTSTTTSFQYGSTEVSQNPAKSPCILPIVYGGVKASSTHYYLLPEKPAYLDKYEKFFNSEGNDEKRTTNMATVRPMVQQQGNKSNASPSHTSNSGTSSSLAWPSSSQAGSYSRVTLQQVQEAVHGVTVEECQTALQAHNWNAQEAVKYLKVEQLFRLGLKTRPECMETLQRCGWNLEQASTQMLDSYGPSRHRF

Target ORF information:

RefSeq Version	XM_021470344.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio activated CDC42 kinase 1-like (LOC100148041), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470344.1

ORF Insert Sequence:

ATGGCGGCGC TCTCAGATTA CCAACGACAC TATAATGTAA AAGACGAGCC GGACATGCAG 
TCTGATGAAG GTACAGAGTG GCTCTTGGAG CTGCTCACAG ATGTGCAGCT TCAGCAATAT 
TTCCAGCGTA TCCGTGATGA GCTCAATGTC ACGCGTCTCT CACACTTTGA CTATGTCAAA 
ACAGAGGACC TCGAAAAAAT TGGCATGGGC CGACCAGGTC AAAGGCGGCT GTGGGAGGCA 
GTCAAAAGAA GAAGGGCAAT GTGCAAACGG AAGTCATGGA TGAGCAAGTC TCTGTGTTTG 
GGTCAGCAGG TGTTTACCGG GAAGCGCCCT GACTCTGATT CGCAGACTCC AGAGGTTTTC 
CGCAGCTCGT CCCCTTCATC GGCTCCTCCG GATGGCCAGC CGACGGATCT CACCTGCCTC 
ATCAGCGAAC GAGACCTCAC TCTCTATGAA AAACTGGGGG ACGGATCTTT CGGAGTGGTG 
AAGCGTGGAG AATGGCAGGC CCCCTCTGGA AGAGTGCTAA GCGTGGCTGT GAAGTGCCTG 
AAGACGGACC TGTTGGACCA GGGTGAAGGT CTAGATGACT TCATACGAGA AGTTAATGCC 
ATGCACTCCT TGAACCACCA CAATCTCATT CGTCTGTATG GGTGCGTCCT CACACACCCC 
ATGAAGATGG TGACAGAACT TGCTCCATTG GGTTCTCTGT TGGACCGCCT GAGGAAACGT 
CAGGGTCATA TACTGATATC AGTGCTGTGC CACTACACTG TTCAGATCGC CTGTGGGATG 
GCATACCTGG AGTCTCGACG CTTCATTCAC AGAGACCTGG CTGCACGCAA CATCCTTTTA 
GCCTCCAATG ACCTCATCAA GATCGGTGAC TTCGGCCTGA TGAGGGCCTT GCCTAAAAAT 
GATGATCATT ACGTTATGCA GGAGCATCAC AAAGTCCCTT TTGCATGGTG TGCTCCAGAG 
AGCCTGAAGA CCCGTACCTT CTCCCATGCC AGTGATACCT GGATGTTTGG AGTAACATTG 
TGGGAAATGT TCACTCACGG GCAGGAACCA TGGGTTGGAC TCAATGGTAG TCAGATCCTC 
CATAAGATCG ATAGAGAGGG AGAAAGGCTT ATCAAACCAG AGGACTGTCC TCAGGACATT 
TATAACGTCA TGCTGCAGTG CTGGTCACCC AAACCTGAGG ACAGGCCAAC ATTTGTGTCC 
CTTAGGGACT TCCTTGTGGA GACAATGCCA ACAGATATGA GGGCACTGCA GGACTTTGAT 
GAGCCAGACA AACTTAAAAT CCACACTAAT GACATCATCA CCATCATTGA GGGCAGAGCA 
GAGAACTACT GGTGGAGGGG TCAGAACAGA CAGACCATGA AGGTGGGACA GTTCCCCAGG 
AATGTAGTGA CCTCTGTGGC CGGTCTATCT GCTCAGGACA TCAGCCGACC TCTCAAGCAC 
AGTTTCATCC ACACAGGCCA TGGAGACACT GACCCACACC GTAGCTGGGG TGCCCCTGAT 
AAGATTGATG ATCTGTACCT TGGTAATCCC ATGGATCCTC CAGATGTTCT TGGGTTGGAT 
TCAAATACAG CCAAGCCCAC TAAGCTACCA AACCGGGCTA AAAAACAACC TCCTCCTAGA 
CCTCCTAAAC CAGCTGTTCT TCTTAAGAAA CCATTTTATG ACTCTGTCCT GGATGATGAT 
GATGATGATG ATCTGATAGT GACAGGTGTG AAGAAGCTCT CACTTAAGAC TCCTACATAC 
CTGGGTTTAC GCCTTCGCCC ATGGGAAAGC ACTAGTCCGA GTGACCGCCT TTGTGAAGTC 
TCCCTTATTG ACTTTGGGGA TGATAGTTTC AGCTCAACAT CGCCCTCTCC CATTACCGAG 
ATTCCCAATC CTAGCACAGC GAAACCACCT TTGTCCTATG CCGCTTCCAT CTTGGATATG 
GCACCACCCC AGAGCCCAAA TCAAGCCTTA CCCAACCCCA TGCACCCTAC TCCAGTGGTA 
GACTGGGACA TGCGGCCCCT GCCTCCTCTC CCGGCATATG ATGAGGTGGC CGTTGAGTGT 
GCAGAGCAGG AAGACATAGA AGTGAGCTCC ATAAATGCAT CTGCAGCCCA GGAAGACACT 
GTATATGAAC AGACCCCATC AGAAGAAGAC ATTAATAATG ATGGTAAACG GAACGTGGAG 
GATAACCTCT TTCTTCCATC AAAGCGTCCT GAGGAATCCC ACTCGTTTTC ACAATCAGCA 
GATATCTTCA AGGAACTGCA AAGAGAGGTG ATGGTAAAGC TTCGGGTCCC TCCAAGTGGG 
CGTTCTCTAC CTTCTTCACC CCTCCCGACT CCTTCAGTTC TCACCCCACA CCGTCAGATA 
ATCCTTCCAT CCTCTTATGA GGACAAGCCT CAGATCCCTC CAAGAATCCC TATTCCACCT 
ATGCGGCCTT CAAAGAGGAC AGGAATATAC GGCAGCAGAC TGTCTGTTTC TCTTGGAGAA 
AATGAAGATG GGAGCCGTTG TCCACCCCAA ATACCCCCGC GCGATCATGC TCTATCTCAG 
CCCAATTCTC GGGCACCAAG TCCTATGGTG CCTCAAAGCG GCTCTCCTCA ACAAAGATCC 
AGCCTGTGCT GTGTTGGATC ATTGGGCTCC TGCTTGTCTC CATCATCATA TTCTTCTGCA 
CCATCCAGCT CGTCCACCAC ATCTTCCACA TCCACCACTA CTAGTTTTCA ATATGGATCT 
ACTGAAGTGA GCCAAAATCC AGCCAAGAGT CCTTGCATCC TGCCAATTGT TTACGGTGGT 
GTAAAGGCCA GCAGCACTCA TTACTACTTG CTACCTGAGA AACCGGCATA CTTGGACAAG 
TATGAGAAGT TCTTTAATTC GGAGGGAAAC GATGAGAAAA GGACTACAAA TATGGCTACT 
GTGAGACCCA TGGTTCAGCA GCAAGGCAAC AAATCCAATG CTTCCCCAAG CCACACCAGT 
AACAGTGGGA CCTCCAGCAG TCTGGCTTGG CCAAGCAGCT CCCAAGCAGG TTCTTACAGC 
CGTGTCACGC TGCAACAAGT ACAGGAAGCA GTACATGGGG TGACTGTAGA GGAGTGTCAA 
ACAGCCTTGC AGGCACACAA CTGGAATGCT CAGGAGGCCG TGAAGTATCT AAAGGTAGAA 
CAGTTATTCC GACTGGGTTT GAAGACTCGG CCTGAATGCA TGGAAACTCT GCAGAGATGT 
GGCTGGAATC TGGAACAGGC CAGCACCCAA ATGCTGGATT CCTATGGTCC ATCTAGGCAT 
AGGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100148041 ( XM_009297483.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295758.1 Danio rerio activated CDC42 kinase 1-like (LOC100148041), transcript variant X1, mRNA.

Price & Availability↑

CloneID	ODa48613
Clone ID Related Accession (Same CDS sequence)	XM_021470344.1 , XM_009297483.2
Accession Version	XM_009297483.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3249bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	activated CDC42 kinase 1-like isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297483.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCGGCGC TCTCAGATTA CCAACGACAC TATAATGTAA AAGACGAGCC GGACATGCAG 
TCTGATGAAG GTACAGAGTG GCTCTTGGAG CTGCTCACAG ATGTGCAGCT TCAGCAATAT 
TTCCAGCGTA TCCGTGATGA GCTCAATGTC ACGCGTCTCT CACACTTTGA CTATGTCAAA 
ACAGAGGACC TCGAAAAAAT TGGCATGGGC CGACCAGGTC AAAGGCGGCT GTGGGAGGCA 
GTCAAAAGAA GAAGGGCAAT GTGCAAACGG AAGTCATGGA TGAGCAAGTC TCTGTGTTTG 
GGTCAGCAGG TGTTTACCGG GAAGCGCCCT GACTCTGATT CGCAGACTCC AGAGGTTTTC 
CGCAGCTCGT CCCCTTCATC GGCTCCTCCG GATGGCCAGC CGACGGATCT CACCTGCCTC 
ATCAGCGAAC GAGACCTCAC TCTCTATGAA AAACTGGGGG ACGGATCTTT CGGAGTGGTG 
AAGCGTGGAG AATGGCAGGC CCCCTCTGGA AGAGTGCTAA GCGTGGCTGT GAAGTGCCTG 
AAGACGGACC TGTTGGACCA GGGTGAAGGT CTAGATGACT TCATACGAGA AGTTAATGCC 
ATGCACTCCT TGAACCACCA CAATCTCATT CGTCTGTATG GGTGCGTCCT CACACACCCC 
ATGAAGATGG TGACAGAACT TGCTCCATTG GGTTCTCTGT TGGACCGCCT GAGGAAACGT 
CAGGGTCATA TACTGATATC AGTGCTGTGC CACTACACTG TTCAGATCGC CTGTGGGATG 
GCATACCTGG AGTCTCGACG CTTCATTCAC AGAGACCTGG CTGCACGCAA CATCCTTTTA 
GCCTCCAATG ACCTCATCAA GATCGGTGAC TTCGGCCTGA TGAGGGCCTT GCCTAAAAAT 
GATGATCATT ACGTTATGCA GGAGCATCAC AAAGTCCCTT TTGCATGGTG TGCTCCAGAG 
AGCCTGAAGA CCCGTACCTT CTCCCATGCC AGTGATACCT GGATGTTTGG AGTAACATTG 
TGGGAAATGT TCACTCACGG GCAGGAACCA TGGGTTGGAC TCAATGGTAG TCAGATCCTC 
CATAAGATCG ATAGAGAGGG AGAAAGGCTT ATCAAACCAG AGGACTGTCC TCAGGACATT 
TATAACGTCA TGCTGCAGTG CTGGTCACCC AAACCTGAGG ACAGGCCAAC ATTTGTGTCC 
CTTAGGGACT TCCTTGTGGA GACAATGCCA ACAGATATGA GGGCACTGCA GGACTTTGAT 
GAGCCAGACA AACTTAAAAT CCACACTAAT GACATCATCA CCATCATTGA GGGCAGAGCA 
GAGAACTACT GGTGGAGGGG TCAGAACAGA CAGACCATGA AGGTGGGACA GTTCCCCAGG 
AATGTAGTGA CCTCTGTGGC CGGTCTATCT GCTCAGGACA TCAGCCGACC TCTCAAGCAC 
AGTTTCATCC ACACAGGCCA TGGAGACACT GACCCACACC GTAGCTGGGG TGCCCCTGAT 
AAGATTGATG ATCTGTACCT TGGTAATCCC ATGGATCCTC CAGATGTTCT TGGGTTGGAT 
TCAAATACAG CCAAGCCCAC TAAGCTACCA AACCGGGCTA AAAAACAACC TCCTCCTAGA 
CCTCCTAAAC CAGCTGTTCT TCTTAAGAAA CCATTTTATG ACTCTGTCCT GGATGATGAT 
GATGATGATG ATCTGATAGT GACAGGTGTG AAGAAGCTCT CACTTAAGAC TCCTACATAC 
CTGGGTTTAC GCCTTCGCCC ATGGGAAAGC ACTAGTCCGA GTGACCGCCT TTGTGAAGTC 
TCCCTTATTG ACTTTGGGGA TGATAGTTTC AGCTCAACAT CGCCCTCTCC CATTACCGAG 
ATTCCCAATC CTAGCACAGC GAAACCACCT TTGTCCTATG CCGCTTCCAT CTTGGATATG 
GCACCACCCC AGAGCCCAAA TCAAGCCTTA CCCAACCCCA TGCACCCTAC TCCAGTGGTA 
GACTGGGACA TGCGGCCCCT GCCTCCTCTC CCGGCATATG ATGAGGTGGC CGTTGAGTGT 
GCAGAGCAGG AAGACATAGA AGTGAGCTCC ATAAATGCAT CTGCAGCCCA GGAAGACACT 
GTATATGAAC AGACCCCATC AGAAGAAGAC ATTAATAATG ATGGTAAACG GAACGTGGAG 
GATAACCTCT TTCTTCCATC AAAGCGTCCT GAGGAATCCC ACTCGTTTTC ACAATCAGCA 
GATATCTTCA AGGAACTGCA AAGAGAGGTG ATGGTAAAGC TTCGGGTCCC TCCAAGTGGG 
CGTTCTCTAC CTTCTTCACC CCTCCCGACT CCTTCAGTTC TCACCCCACA CCGTCAGATA 
ATCCTTCCAT CCTCTTATGA GGACAAGCCT CAGATCCCTC CAAGAATCCC TATTCCACCT 
ATGCGGCCTT CAAAGAGGAC AGGAATATAC GGCAGCAGAC TGTCTGTTTC TCTTGGAGAA 
AATGAAGATG GGAGCCGTTG TCCACCCCAA ATACCCCCGC GCGATCATGC TCTATCTCAG 
CCCAATTCTC GGGCACCAAG TCCTATGGTG CCTCAAAGCG GCTCTCCTCA ACAAAGATCC 
AGCCTGTGCT GTGTTGGATC ATTGGGCTCC TGCTTGTCTC CATCATCATA TTCTTCTGCA 
CCATCCAGCT CGTCCACCAC ATCTTCCACA TCCACCACTA CTAGTTTTCA ATATGGATCT 
ACTGAAGTGA GCCAAAATCC AGCCAAGAGT CCTTGCATCC TGCCAATTGT TTACGGTGGT 
GTAAAGGCCA GCAGCACTCA TTACTACTTG CTACCTGAGA AACCGGCATA CTTGGACAAG 
TATGAGAAGT TCTTTAATTC GGAGGGAAAC GATGAGAAAA GGACTACAAA TATGGCTACT 
GTGAGACCCA TGGTTCAGCA GCAAGGCAAC AAATCCAATG CTTCCCCAAG CCACACCAGT 
AACAGTGGGA CCTCCAGCAG TCTGGCTTGG CCAAGCAGCT CCCAAGCAGG TTCTTACAGC 
CGTGTCACGC TGCAACAAGT ACAGGAAGCA GTACATGGGG TGACTGTAGA GGAGTGTCAA 
ACAGCCTTGC AGGCACACAA CTGGAATGCT CAGGAGGCCG TGAAGTATCT AAAGGTAGAA 
CAGTTATTCC GACTGGGTTT GAAGACTCGG CCTGAATGCA TGGAAACTCT GCAGAGATGT 
GGCTGGAATC TGGAACAGGC CAGCACCCAA ATGCTGGATT CCTATGGTCC ATCTAGGCAT 
AGGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295758.1
CDS	619..3867
Translation	MAALSDYQRHYNVKDEPDMQSDEGTEWLLELLTDVQLQQYFQRIRDELNVTRLSHFDYVKTEDLEKIGMGRPGQRRLWEAVKRRRAMCKRKSWMSKSLCLGQQVFTGKRPDSDSQTPEVFRSSSPSSAPPDGQPTDLTCLISERDLTLYEKLGDGSFGVVKRGEWQAPSGRVLSVAVKCLKTDLLDQGEGLDDFIREVNAMHSLNHHNLIRLYGCVLTHPMKMVTELAPLGSLLDRLRKRQGHILISVLCHYTVQIACGMAYLESRRFIHRDLAARNILLASNDLIKIGDFGLMRALPKNDDHYVMQEHHKVPFAWCAPESLKTRTFSHASDTWMFGVTLWEMFTHGQEPWVGLNGSQILHKIDREGERLIKPEDCPQDIYNVMLQCWSPKPEDRPTFVSLRDFLVETMPTDMRALQDFDEPDKLKIHTNDIITIIEGRAENYWWRGQNRQTMKVGQFPRNVVTSVAGLSAQDISRPLKHSFIHTGHGDTDPHRSWGAPDKIDDLYLGNPMDPPDVLGLDSNTAKPTKLPNRAKKQPPPRPPKPAVLLKKPFYDSVLDDDDDDDLIVTGVKKLSLKTPTYLGLRLRPWESTSPSDRLCEVSLIDFGDDSFSSTSPSPITEIPNPSTAKPPLSYAASILDMAPPQSPNQALPNPMHPTPVVDWDMRPLPPLPAYDEVAVECAEQEDIEVSSINASAAQEDTVYEQTPSEEDINNDGKRNVEDNLFLPSKRPEESHSFSQSADIFKELQREVMVKLRVPPSGRSLPSSPLPTPSVLTPHRQIILPSSYEDKPQIPPRIPIPPMRPSKRTGIYGSRLSVSLGENEDGSRCPPQIPPRDHALSQPNSRAPSPMVPQSGSPQQRSSLCCVGSLGSCLSPSSYSSAPSSSSTTSSTSTTTSFQYGSTEVSQNPAKSPCILPIVYGGVKASSTHYYLLPEKPAYLDKYEKFFNSEGNDEKRTTNMATVRPMVQQQGNKSNASPSHTSNSGTSSSLAWPSSSQAGSYSRVTLQQVQEAVHGVTVEECQTALQAHNWNAQEAVKYLKVEQLFRLGLKTRPECMETLQRCGWNLEQASTQMLDSYGPSRHRF

Target ORF information:

RefSeq Version	XM_009297483.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio activated CDC42 kinase 1-like (LOC100148041), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297483.2

ORF Insert Sequence:

ATGGCGGCGC TCTCAGATTA CCAACGACAC TATAATGTAA AAGACGAGCC GGACATGCAG 
TCTGATGAAG GTACAGAGTG GCTCTTGGAG CTGCTCACAG ATGTGCAGCT TCAGCAATAT 
TTCCAGCGTA TCCGTGATGA GCTCAATGTC ACGCGTCTCT CACACTTTGA CTATGTCAAA 
ACAGAGGACC TCGAAAAAAT TGGCATGGGC CGACCAGGTC AAAGGCGGCT GTGGGAGGCA 
GTCAAAAGAA GAAGGGCAAT GTGCAAACGG AAGTCATGGA TGAGCAAGTC TCTGTGTTTG 
GGTCAGCAGG TGTTTACCGG GAAGCGCCCT GACTCTGATT CGCAGACTCC AGAGGTTTTC 
CGCAGCTCGT CCCCTTCATC GGCTCCTCCG GATGGCCAGC CGACGGATCT CACCTGCCTC 
ATCAGCGAAC GAGACCTCAC TCTCTATGAA AAACTGGGGG ACGGATCTTT CGGAGTGGTG 
AAGCGTGGAG AATGGCAGGC CCCCTCTGGA AGAGTGCTAA GCGTGGCTGT GAAGTGCCTG 
AAGACGGACC TGTTGGACCA GGGTGAAGGT CTAGATGACT TCATACGAGA AGTTAATGCC 
ATGCACTCCT TGAACCACCA CAATCTCATT CGTCTGTATG GGTGCGTCCT CACACACCCC 
ATGAAGATGG TGACAGAACT TGCTCCATTG GGTTCTCTGT TGGACCGCCT GAGGAAACGT 
CAGGGTCATA TACTGATATC AGTGCTGTGC CACTACACTG TTCAGATCGC CTGTGGGATG 
GCATACCTGG AGTCTCGACG CTTCATTCAC AGAGACCTGG CTGCACGCAA CATCCTTTTA 
GCCTCCAATG ACCTCATCAA GATCGGTGAC TTCGGCCTGA TGAGGGCCTT GCCTAAAAAT 
GATGATCATT ACGTTATGCA GGAGCATCAC AAAGTCCCTT TTGCATGGTG TGCTCCAGAG 
AGCCTGAAGA CCCGTACCTT CTCCCATGCC AGTGATACCT GGATGTTTGG AGTAACATTG 
TGGGAAATGT TCACTCACGG GCAGGAACCA TGGGTTGGAC TCAATGGTAG TCAGATCCTC 
CATAAGATCG ATAGAGAGGG AGAAAGGCTT ATCAAACCAG AGGACTGTCC TCAGGACATT 
TATAACGTCA TGCTGCAGTG CTGGTCACCC AAACCTGAGG ACAGGCCAAC ATTTGTGTCC 
CTTAGGGACT TCCTTGTGGA GACAATGCCA ACAGATATGA GGGCACTGCA GGACTTTGAT 
GAGCCAGACA AACTTAAAAT CCACACTAAT GACATCATCA CCATCATTGA GGGCAGAGCA 
GAGAACTACT GGTGGAGGGG TCAGAACAGA CAGACCATGA AGGTGGGACA GTTCCCCAGG 
AATGTAGTGA CCTCTGTGGC CGGTCTATCT GCTCAGGACA TCAGCCGACC TCTCAAGCAC 
AGTTTCATCC ACACAGGCCA TGGAGACACT GACCCACACC GTAGCTGGGG TGCCCCTGAT 
AAGATTGATG ATCTGTACCT TGGTAATCCC ATGGATCCTC CAGATGTTCT TGGGTTGGAT 
TCAAATACAG CCAAGCCCAC TAAGCTACCA AACCGGGCTA AAAAACAACC TCCTCCTAGA 
CCTCCTAAAC CAGCTGTTCT TCTTAAGAAA CCATTTTATG ACTCTGTCCT GGATGATGAT 
GATGATGATG ATCTGATAGT GACAGGTGTG AAGAAGCTCT CACTTAAGAC TCCTACATAC 
CTGGGTTTAC GCCTTCGCCC ATGGGAAAGC ACTAGTCCGA GTGACCGCCT TTGTGAAGTC 
TCCCTTATTG ACTTTGGGGA TGATAGTTTC AGCTCAACAT CGCCCTCTCC CATTACCGAG 
ATTCCCAATC CTAGCACAGC GAAACCACCT TTGTCCTATG CCGCTTCCAT CTTGGATATG 
GCACCACCCC AGAGCCCAAA TCAAGCCTTA CCCAACCCCA TGCACCCTAC TCCAGTGGTA 
GACTGGGACA TGCGGCCCCT GCCTCCTCTC CCGGCATATG ATGAGGTGGC CGTTGAGTGT 
GCAGAGCAGG AAGACATAGA AGTGAGCTCC ATAAATGCAT CTGCAGCCCA GGAAGACACT 
GTATATGAAC AGACCCCATC AGAAGAAGAC ATTAATAATG ATGGTAAACG GAACGTGGAG 
GATAACCTCT TTCTTCCATC AAAGCGTCCT GAGGAATCCC ACTCGTTTTC ACAATCAGCA 
GATATCTTCA AGGAACTGCA AAGAGAGGTG ATGGTAAAGC TTCGGGTCCC TCCAAGTGGG 
CGTTCTCTAC CTTCTTCACC CCTCCCGACT CCTTCAGTTC TCACCCCACA CCGTCAGATA 
ATCCTTCCAT CCTCTTATGA GGACAAGCCT CAGATCCCTC CAAGAATCCC TATTCCACCT 
ATGCGGCCTT CAAAGAGGAC AGGAATATAC GGCAGCAGAC TGTCTGTTTC TCTTGGAGAA 
AATGAAGATG GGAGCCGTTG TCCACCCCAA ATACCCCCGC GCGATCATGC TCTATCTCAG 
CCCAATTCTC GGGCACCAAG TCCTATGGTG CCTCAAAGCG GCTCTCCTCA ACAAAGATCC 
AGCCTGTGCT GTGTTGGATC ATTGGGCTCC TGCTTGTCTC CATCATCATA TTCTTCTGCA 
CCATCCAGCT CGTCCACCAC ATCTTCCACA TCCACCACTA CTAGTTTTCA ATATGGATCT 
ACTGAAGTGA GCCAAAATCC AGCCAAGAGT CCTTGCATCC TGCCAATTGT TTACGGTGGT 
GTAAAGGCCA GCAGCACTCA TTACTACTTG CTACCTGAGA AACCGGCATA CTTGGACAAG 
TATGAGAAGT TCTTTAATTC GGAGGGAAAC GATGAGAAAA GGACTACAAA TATGGCTACT 
GTGAGACCCA TGGTTCAGCA GCAAGGCAAC AAATCCAATG CTTCCCCAAG CCACACCAGT 
AACAGTGGGA CCTCCAGCAG TCTGGCTTGG CCAAGCAGCT CCCAAGCAGG TTCTTACAGC 
CGTGTCACGC TGCAACAAGT ACAGGAAGCA GTACATGGGG TGACTGTAGA GGAGTGTCAA 
ACAGCCTTGC AGGCACACAA CTGGAATGCT CAGGAGGCCG TGAAGTATCT AAAGGTAGAA 
CAGTTATTCC GACTGGGTTT GAAGACTCGG CCTGAATGCA TGGAAACTCT GCAGAGATGT 
GGCTGGAATC TGGAACAGGC CAGCACCCAA ATGCTGGATT CCTATGGTCC ATCTAGGCAT 
AGGTTCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100148041 ( XM_021470345.1 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_021326020.1 Danio rerio activated CDC42 kinase 1-like (LOC100148041), transcript variant X2, mRNA.

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CloneID	ODa48614
Clone ID Related Accession (Same CDS sequence)	XM_021470345.1 , XM_009297485.2
Accession Version	XM_021470345.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3195bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	activated CDC42 kinase 1-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCTG ATGAAGGTAC AGAGTGGCTC TTGGAGCTGC TCACAGATGT GCAGCTTCAG 
CAATATTTCC AGCGTATCCG TGATGAGCTC AATGTCACGC GTCTCTCACA CTTTGACTAT 
GTCAAAACAG AGGACCTCGA AAAAATTGGC ATGGGCCGAC CAGGTCAAAG GCGGCTGTGG 
GAGGCAGTCA AAAGAAGAAG GGCAATGTGC AAACGGAAGT CATGGATGAG CAAGTCTCTG 
TGTTTGGGTC AGCAGGTGTT TACCGGGAAG CGCCCTGACT CTGATTCGCA GACTCCAGAG 
GTTTTCCGCA GCTCGTCCCC TTCATCGGCT CCTCCGGATG GCCAGCCGAC GGATCTCACC 
TGCCTCATCA GCGAACGAGA CCTCACTCTC TATGAAAAAC TGGGGGACGG ATCTTTCGGA 
GTGGTGAAGC GTGGAGAATG GCAGGCCCCC TCTGGAAGAG TGCTAAGCGT GGCTGTGAAG 
TGCCTGAAGA CGGACCTGTT GGACCAGGGT GAAGGTCTAG ATGACTTCAT ACGAGAAGTT 
AATGCCATGC ACTCCTTGAA CCACCACAAT CTCATTCGTC TGTATGGGTG CGTCCTCACA 
CACCCCATGA AGATGGTGAC AGAACTTGCT CCATTGGGTT CTCTGTTGGA CCGCCTGAGG 
AAACGTCAGG GTCATATACT GATATCAGTG CTGTGCCACT ACACTGTTCA GATCGCCTGT 
GGGATGGCAT ACCTGGAGTC TCGACGCTTC ATTCACAGAG ACCTGGCTGC ACGCAACATC 
CTTTTAGCCT CCAATGACCT CATCAAGATC GGTGACTTCG GCCTGATGAG GGCCTTGCCT 
AAAAATGATG ATCATTACGT TATGCAGGAG CATCACAAAG TCCCTTTTGC ATGGTGTGCT 
CCAGAGAGCC TGAAGACCCG TACCTTCTCC CATGCCAGTG ATACCTGGAT GTTTGGAGTA 
ACATTGTGGG AAATGTTCAC TCACGGGCAG GAACCATGGG TTGGACTCAA TGGTAGTCAG 
ATCCTCCATA AGATCGATAG AGAGGGAGAA AGGCTTATCA AACCAGAGGA CTGTCCTCAG 
GACATTTATA ACGTCATGCT GCAGTGCTGG TCACCCAAAC CTGAGGACAG GCCAACATTT 
GTGTCCCTTA GGGACTTCCT TGTGGAGACA ATGCCAACAG ATATGAGGGC ACTGCAGGAC 
TTTGATGAGC CAGACAAACT TAAAATCCAC ACTAATGACA TCATCACCAT CATTGAGGGC 
AGAGCAGAGA ACTACTGGTG GAGGGGTCAG AACAGACAGA CCATGAAGGT GGGACAGTTC 
CCCAGGAATG TAGTGACCTC TGTGGCCGGT CTATCTGCTC AGGACATCAG CCGACCTCTC 
AAGCACAGTT TCATCCACAC AGGCCATGGA GACACTGACC CACACCGTAG CTGGGGTGCC 
CCTGATAAGA TTGATGATCT GTACCTTGGT AATCCCATGG ATCCTCCAGA TGTTCTTGGG 
TTGGATTCAA ATACAGCCAA GCCCACTAAG CTACCAAACC GGGCTAAAAA ACAACCTCCT 
CCTAGACCTC CTAAACCAGC TGTTCTTCTT AAGAAACCAT TTTATGACTC TGTCCTGGAT 
GATGATGATG ATGATGATCT GATAGTGACA GGTGTGAAGA AGCTCTCACT TAAGACTCCT 
ACATACCTGG GTTTACGCCT TCGCCCATGG GAAAGCACTA GTCCGAGTGA CCGCCTTTGT 
GAAGTCTCCC TTATTGACTT TGGGGATGAT AGTTTCAGCT CAACATCGCC CTCTCCCATT 
ACCGAGATTC CCAATCCTAG CACAGCGAAA CCACCTTTGT CCTATGCCGC TTCCATCTTG 
GATATGGCAC CACCCCAGAG CCCAAATCAA GCCTTACCCA ACCCCATGCA CCCTACTCCA 
GTGGTAGACT GGGACATGCG GCCCCTGCCT CCTCTCCCGG CATATGATGA GGTGGCCGTT 
GAGTGTGCAG AGCAGGAAGA CATAGAAGTG AGCTCCATAA ATGCATCTGC AGCCCAGGAA 
GACACTGTAT ATGAACAGAC CCCATCAGAA GAAGACATTA ATAATGATGG TAAACGGAAC 
GTGGAGGATA ACCTCTTTCT TCCATCAAAG CGTCCTGAGG AATCCCACTC GTTTTCACAA 
TCAGCAGATA TCTTCAAGGA ACTGCAAAGA GAGGTGATGG TAAAGCTTCG GGTCCCTCCA 
AGTGGGCGTT CTCTACCTTC TTCACCCCTC CCGACTCCTT CAGTTCTCAC CCCACACCGT 
CAGATAATCC TTCCATCCTC TTATGAGGAC AAGCCTCAGA TCCCTCCAAG AATCCCTATT 
CCACCTATGC GGCCTTCAAA GAGGACAGGA ATATACGGCA GCAGACTGTC TGTTTCTCTT 
GGAGAAAATG AAGATGGGAG CCGTTGTCCA CCCCAAATAC CCCCGCGCGA TCATGCTCTA 
TCTCAGCCCA ATTCTCGGGC ACCAAGTCCT ATGGTGCCTC AAAGCGGCTC TCCTCAACAA 
AGATCCAGCC TGTGCTGTGT TGGATCATTG GGCTCCTGCT TGTCTCCATC ATCATATTCT 
TCTGCACCAT CCAGCTCGTC CACCACATCT TCCACATCCA CCACTACTAG TTTTCAATAT 
GGATCTACTG AAGTGAGCCA AAATCCAGCC AAGAGTCCTT GCATCCTGCC AATTGTTTAC 
GGTGGTGTAA AGGCCAGCAG CACTCATTAC TACTTGCTAC CTGAGAAACC GGCATACTTG 
GACAAGTATG AGAAGTTCTT TAATTCGGAG GGAAACGATG AGAAAAGGAC TACAAATATG 
GCTACTGTGA GACCCATGGT TCAGCAGCAA GGCAACAAAT CCAATGCTTC CCCAAGCCAC 
ACCAGTAACA GTGGGACCTC CAGCAGTCTG GCTTGGCCAA GCAGCTCCCA AGCAGGTTCT 
TACAGCCGTG TCACGCTGCA ACAAGTACAG GAAGCAGTAC ATGGGGTGAC TGTAGAGGAG 
TGTCAAACAG CCTTGCAGGC ACACAACTGG AATGCTCAGG AGGCCGTGAA GTATCTAAAG 
GTAGAACAGT TATTCCGACT GGGTTTGAAG ACTCGGCCTG AATGCATGGA AACTCTGCAG 
AGATGTGGCT GGAATCTGGA ACAGGCCAGC ACCCAAATGC TGGATTCCTA TGGTCCATCT 
AGGCATAGGT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_021326020.1
CDS	260..3454
Translation	MQSDEGTEWLLELLTDVQLQQYFQRIRDELNVTRLSHFDYVKTEDLEKIGMGRPGQRRLWEAVKRRRAMCKRKSWMSKSLCLGQQVFTGKRPDSDSQTPEVFRSSSPSSAPPDGQPTDLTCLISERDLTLYEKLGDGSFGVVKRGEWQAPSGRVLSVAVKCLKTDLLDQGEGLDDFIREVNAMHSLNHHNLIRLYGCVLTHPMKMVTELAPLGSLLDRLRKRQGHILISVLCHYTVQIACGMAYLESRRFIHRDLAARNILLASNDLIKIGDFGLMRALPKNDDHYVMQEHHKVPFAWCAPESLKTRTFSHASDTWMFGVTLWEMFTHGQEPWVGLNGSQILHKIDREGERLIKPEDCPQDIYNVMLQCWSPKPEDRPTFVSLRDFLVETMPTDMRALQDFDEPDKLKIHTNDIITIIEGRAENYWWRGQNRQTMKVGQFPRNVVTSVAGLSAQDISRPLKHSFIHTGHGDTDPHRSWGAPDKIDDLYLGNPMDPPDVLGLDSNTAKPTKLPNRAKKQPPPRPPKPAVLLKKPFYDSVLDDDDDDDLIVTGVKKLSLKTPTYLGLRLRPWESTSPSDRLCEVSLIDFGDDSFSSTSPSPITEIPNPSTAKPPLSYAASILDMAPPQSPNQALPNPMHPTPVVDWDMRPLPPLPAYDEVAVECAEQEDIEVSSINASAAQEDTVYEQTPSEEDINNDGKRNVEDNLFLPSKRPEESHSFSQSADIFKELQREVMVKLRVPPSGRSLPSSPLPTPSVLTPHRQIILPSSYEDKPQIPPRIPIPPMRPSKRTGIYGSRLSVSLGENEDGSRCPPQIPPRDHALSQPNSRAPSPMVPQSGSPQQRSSLCCVGSLGSCLSPSSYSSAPSSSSTTSSTSTTTSFQYGSTEVSQNPAKSPCILPIVYGGVKASSTHYYLLPEKPAYLDKYEKFFNSEGNDEKRTTNMATVRPMVQQQGNKSNASPSHTSNSGTSSSLAWPSSSQAGSYSRVTLQQVQEAVHGVTVEECQTALQAHNWNAQEAVKYLKVEQLFRLGLKTRPECMETLQRCGWNLEQASTQMLDSYGPSRHRF

Target ORF information:

RefSeq Version	XM_021470345.1
Organism	Danio rerio(zebrafish)
Definition	Danio rerio activated CDC42 kinase 1-like (LOC100148041), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_021470345.1

ORF Insert Sequence:

ATGCAGTCTG ATGAAGGTAC AGAGTGGCTC TTGGAGCTGC TCACAGATGT GCAGCTTCAG 
CAATATTTCC AGCGTATCCG TGATGAGCTC AATGTCACGC GTCTCTCACA CTTTGACTAT 
GTCAAAACAG AGGACCTCGA AAAAATTGGC ATGGGCCGAC CAGGTCAAAG GCGGCTGTGG 
GAGGCAGTCA AAAGAAGAAG GGCAATGTGC AAACGGAAGT CATGGATGAG CAAGTCTCTG 
TGTTTGGGTC AGCAGGTGTT TACCGGGAAG CGCCCTGACT CTGATTCGCA GACTCCAGAG 
GTTTTCCGCA GCTCGTCCCC TTCATCGGCT CCTCCGGATG GCCAGCCGAC GGATCTCACC 
TGCCTCATCA GCGAACGAGA CCTCACTCTC TATGAAAAAC TGGGGGACGG ATCTTTCGGA 
GTGGTGAAGC GTGGAGAATG GCAGGCCCCC TCTGGAAGAG TGCTAAGCGT GGCTGTGAAG 
TGCCTGAAGA CGGACCTGTT GGACCAGGGT GAAGGTCTAG ATGACTTCAT ACGAGAAGTT 
AATGCCATGC ACTCCTTGAA CCACCACAAT CTCATTCGTC TGTATGGGTG CGTCCTCACA 
CACCCCATGA AGATGGTGAC AGAACTTGCT CCATTGGGTT CTCTGTTGGA CCGCCTGAGG 
AAACGTCAGG GTCATATACT GATATCAGTG CTGTGCCACT ACACTGTTCA GATCGCCTGT 
GGGATGGCAT ACCTGGAGTC TCGACGCTTC ATTCACAGAG ACCTGGCTGC ACGCAACATC 
CTTTTAGCCT CCAATGACCT CATCAAGATC GGTGACTTCG GCCTGATGAG GGCCTTGCCT 
AAAAATGATG ATCATTACGT TATGCAGGAG CATCACAAAG TCCCTTTTGC ATGGTGTGCT 
CCAGAGAGCC TGAAGACCCG TACCTTCTCC CATGCCAGTG ATACCTGGAT GTTTGGAGTA 
ACATTGTGGG AAATGTTCAC TCACGGGCAG GAACCATGGG TTGGACTCAA TGGTAGTCAG 
ATCCTCCATA AGATCGATAG AGAGGGAGAA AGGCTTATCA AACCAGAGGA CTGTCCTCAG 
GACATTTATA ACGTCATGCT GCAGTGCTGG TCACCCAAAC CTGAGGACAG GCCAACATTT 
GTGTCCCTTA GGGACTTCCT TGTGGAGACA ATGCCAACAG ATATGAGGGC ACTGCAGGAC 
TTTGATGAGC CAGACAAACT TAAAATCCAC ACTAATGACA TCATCACCAT CATTGAGGGC 
AGAGCAGAGA ACTACTGGTG GAGGGGTCAG AACAGACAGA CCATGAAGGT GGGACAGTTC 
CCCAGGAATG TAGTGACCTC TGTGGCCGGT CTATCTGCTC AGGACATCAG CCGACCTCTC 
AAGCACAGTT TCATCCACAC AGGCCATGGA GACACTGACC CACACCGTAG CTGGGGTGCC 
CCTGATAAGA TTGATGATCT GTACCTTGGT AATCCCATGG ATCCTCCAGA TGTTCTTGGG 
TTGGATTCAA ATACAGCCAA GCCCACTAAG CTACCAAACC GGGCTAAAAA ACAACCTCCT 
CCTAGACCTC CTAAACCAGC TGTTCTTCTT AAGAAACCAT TTTATGACTC TGTCCTGGAT 
GATGATGATG ATGATGATCT GATAGTGACA GGTGTGAAGA AGCTCTCACT TAAGACTCCT 
ACATACCTGG GTTTACGCCT TCGCCCATGG GAAAGCACTA GTCCGAGTGA CCGCCTTTGT 
GAAGTCTCCC TTATTGACTT TGGGGATGAT AGTTTCAGCT CAACATCGCC CTCTCCCATT 
ACCGAGATTC CCAATCCTAG CACAGCGAAA CCACCTTTGT CCTATGCCGC TTCCATCTTG 
GATATGGCAC CACCCCAGAG CCCAAATCAA GCCTTACCCA ACCCCATGCA CCCTACTCCA 
GTGGTAGACT GGGACATGCG GCCCCTGCCT CCTCTCCCGG CATATGATGA GGTGGCCGTT 
GAGTGTGCAG AGCAGGAAGA CATAGAAGTG AGCTCCATAA ATGCATCTGC AGCCCAGGAA 
GACACTGTAT ATGAACAGAC CCCATCAGAA GAAGACATTA ATAATGATGG TAAACGGAAC 
GTGGAGGATA ACCTCTTTCT TCCATCAAAG CGTCCTGAGG AATCCCACTC GTTTTCACAA 
TCAGCAGATA TCTTCAAGGA ACTGCAAAGA GAGGTGATGG TAAAGCTTCG GGTCCCTCCA 
AGTGGGCGTT CTCTACCTTC TTCACCCCTC CCGACTCCTT CAGTTCTCAC CCCACACCGT 
CAGATAATCC TTCCATCCTC TTATGAGGAC AAGCCTCAGA TCCCTCCAAG AATCCCTATT 
CCACCTATGC GGCCTTCAAA GAGGACAGGA ATATACGGCA GCAGACTGTC TGTTTCTCTT 
GGAGAAAATG AAGATGGGAG CCGTTGTCCA CCCCAAATAC CCCCGCGCGA TCATGCTCTA 
TCTCAGCCCA ATTCTCGGGC ACCAAGTCCT ATGGTGCCTC AAAGCGGCTC TCCTCAACAA 
AGATCCAGCC TGTGCTGTGT TGGATCATTG GGCTCCTGCT TGTCTCCATC ATCATATTCT 
TCTGCACCAT CCAGCTCGTC CACCACATCT TCCACATCCA CCACTACTAG TTTTCAATAT 
GGATCTACTG AAGTGAGCCA AAATCCAGCC AAGAGTCCTT GCATCCTGCC AATTGTTTAC 
GGTGGTGTAA AGGCCAGCAG CACTCATTAC TACTTGCTAC CTGAGAAACC GGCATACTTG 
GACAAGTATG AGAAGTTCTT TAATTCGGAG GGAAACGATG AGAAAAGGAC TACAAATATG 
GCTACTGTGA GACCCATGGT TCAGCAGCAA GGCAACAAAT CCAATGCTTC CCCAAGCCAC 
ACCAGTAACA GTGGGACCTC CAGCAGTCTG GCTTGGCCAA GCAGCTCCCA AGCAGGTTCT 
TACAGCCGTG TCACGCTGCA ACAAGTACAG GAAGCAGTAC ATGGGGTGAC TGTAGAGGAG 
TGTCAAACAG CCTTGCAGGC ACACAACTGG AATGCTCAGG AGGCCGTGAA GTATCTAAAG 
GTAGAACAGT TATTCCGACT GGGTTTGAAG ACTCGGCCTG AATGCATGGA AACTCTGCAG 
AGATGTGGCT GGAATCTGGA ACAGGCCAGC ACCCAAATGC TGGATTCCTA TGGTCCATCT 
AGGCATAGGT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC100148041 ( XM_009297485.2 ) cDNA ORF clone, Danio rerio(zebrafish) -> XP_009295760.1 Danio rerio activated CDC42 kinase 1-like (LOC100148041), transcript variant X3, mRNA.

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CloneID	ODa48614
Clone ID Related Accession (Same CDS sequence)	XM_021470345.1 , XM_009297485.2
Accession Version	XM_009297485.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3195bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	activated CDC42 kinase 1-like isoform X3
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007135.6) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_009297485.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAGTCTG ATGAAGGTAC AGAGTGGCTC TTGGAGCTGC TCACAGATGT GCAGCTTCAG 
CAATATTTCC AGCGTATCCG TGATGAGCTC AATGTCACGC GTCTCTCACA CTTTGACTAT 
GTCAAAACAG AGGACCTCGA AAAAATTGGC ATGGGCCGAC CAGGTCAAAG GCGGCTGTGG 
GAGGCAGTCA AAAGAAGAAG GGCAATGTGC AAACGGAAGT CATGGATGAG CAAGTCTCTG 
TGTTTGGGTC AGCAGGTGTT TACCGGGAAG CGCCCTGACT CTGATTCGCA GACTCCAGAG 
GTTTTCCGCA GCTCGTCCCC TTCATCGGCT CCTCCGGATG GCCAGCCGAC GGATCTCACC 
TGCCTCATCA GCGAACGAGA CCTCACTCTC TATGAAAAAC TGGGGGACGG ATCTTTCGGA 
GTGGTGAAGC GTGGAGAATG GCAGGCCCCC TCTGGAAGAG TGCTAAGCGT GGCTGTGAAG 
TGCCTGAAGA CGGACCTGTT GGACCAGGGT GAAGGTCTAG ATGACTTCAT ACGAGAAGTT 
AATGCCATGC ACTCCTTGAA CCACCACAAT CTCATTCGTC TGTATGGGTG CGTCCTCACA 
CACCCCATGA AGATGGTGAC AGAACTTGCT CCATTGGGTT CTCTGTTGGA CCGCCTGAGG 
AAACGTCAGG GTCATATACT GATATCAGTG CTGTGCCACT ACACTGTTCA GATCGCCTGT 
GGGATGGCAT ACCTGGAGTC TCGACGCTTC ATTCACAGAG ACCTGGCTGC ACGCAACATC 
CTTTTAGCCT CCAATGACCT CATCAAGATC GGTGACTTCG GCCTGATGAG GGCCTTGCCT 
AAAAATGATG ATCATTACGT TATGCAGGAG CATCACAAAG TCCCTTTTGC ATGGTGTGCT 
CCAGAGAGCC TGAAGACCCG TACCTTCTCC CATGCCAGTG ATACCTGGAT GTTTGGAGTA 
ACATTGTGGG AAATGTTCAC TCACGGGCAG GAACCATGGG TTGGACTCAA TGGTAGTCAG 
ATCCTCCATA AGATCGATAG AGAGGGAGAA AGGCTTATCA AACCAGAGGA CTGTCCTCAG 
GACATTTATA ACGTCATGCT GCAGTGCTGG TCACCCAAAC CTGAGGACAG GCCAACATTT 
GTGTCCCTTA GGGACTTCCT TGTGGAGACA ATGCCAACAG ATATGAGGGC ACTGCAGGAC 
TTTGATGAGC CAGACAAACT TAAAATCCAC ACTAATGACA TCATCACCAT CATTGAGGGC 
AGAGCAGAGA ACTACTGGTG GAGGGGTCAG AACAGACAGA CCATGAAGGT GGGACAGTTC 
CCCAGGAATG TAGTGACCTC TGTGGCCGGT CTATCTGCTC AGGACATCAG CCGACCTCTC 
AAGCACAGTT TCATCCACAC AGGCCATGGA GACACTGACC CACACCGTAG CTGGGGTGCC 
CCTGATAAGA TTGATGATCT GTACCTTGGT AATCCCATGG ATCCTCCAGA TGTTCTTGGG 
TTGGATTCAA ATACAGCCAA GCCCACTAAG CTACCAAACC GGGCTAAAAA ACAACCTCCT 
CCTAGACCTC CTAAACCAGC TGTTCTTCTT AAGAAACCAT TTTATGACTC TGTCCTGGAT 
GATGATGATG ATGATGATCT GATAGTGACA GGTGTGAAGA AGCTCTCACT TAAGACTCCT 
ACATACCTGG GTTTACGCCT TCGCCCATGG GAAAGCACTA GTCCGAGTGA CCGCCTTTGT 
GAAGTCTCCC TTATTGACTT TGGGGATGAT AGTTTCAGCT CAACATCGCC CTCTCCCATT 
ACCGAGATTC CCAATCCTAG CACAGCGAAA CCACCTTTGT CCTATGCCGC TTCCATCTTG 
GATATGGCAC CACCCCAGAG CCCAAATCAA GCCTTACCCA ACCCCATGCA CCCTACTCCA 
GTGGTAGACT GGGACATGCG GCCCCTGCCT CCTCTCCCGG CATATGATGA GGTGGCCGTT 
GAGTGTGCAG AGCAGGAAGA CATAGAAGTG AGCTCCATAA ATGCATCTGC AGCCCAGGAA 
GACACTGTAT ATGAACAGAC CCCATCAGAA GAAGACATTA ATAATGATGG TAAACGGAAC 
GTGGAGGATA ACCTCTTTCT TCCATCAAAG CGTCCTGAGG AATCCCACTC GTTTTCACAA 
TCAGCAGATA TCTTCAAGGA ACTGCAAAGA GAGGTGATGG TAAAGCTTCG GGTCCCTCCA 
AGTGGGCGTT CTCTACCTTC TTCACCCCTC CCGACTCCTT CAGTTCTCAC CCCACACCGT 
CAGATAATCC TTCCATCCTC TTATGAGGAC AAGCCTCAGA TCCCTCCAAG AATCCCTATT 
CCACCTATGC GGCCTTCAAA GAGGACAGGA ATATACGGCA GCAGACTGTC TGTTTCTCTT 
GGAGAAAATG AAGATGGGAG CCGTTGTCCA CCCCAAATAC CCCCGCGCGA TCATGCTCTA 
TCTCAGCCCA ATTCTCGGGC ACCAAGTCCT ATGGTGCCTC AAAGCGGCTC TCCTCAACAA 
AGATCCAGCC TGTGCTGTGT TGGATCATTG GGCTCCTGCT TGTCTCCATC ATCATATTCT 
TCTGCACCAT CCAGCTCGTC CACCACATCT TCCACATCCA CCACTACTAG TTTTCAATAT 
GGATCTACTG AAGTGAGCCA AAATCCAGCC AAGAGTCCTT GCATCCTGCC AATTGTTTAC 
GGTGGTGTAA AGGCCAGCAG CACTCATTAC TACTTGCTAC CTGAGAAACC GGCATACTTG 
GACAAGTATG AGAAGTTCTT TAATTCGGAG GGAAACGATG AGAAAAGGAC TACAAATATG 
GCTACTGTGA GACCCATGGT TCAGCAGCAA GGCAACAAAT CCAATGCTTC CCCAAGCCAC 
ACCAGTAACA GTGGGACCTC CAGCAGTCTG GCTTGGCCAA GCAGCTCCCA AGCAGGTTCT 
TACAGCCGTG TCACGCTGCA ACAAGTACAG GAAGCAGTAC ATGGGGTGAC TGTAGAGGAG 
TGTCAAACAG CCTTGCAGGC ACACAACTGG AATGCTCAGG AGGCCGTGAA GTATCTAAAG 
GTAGAACAGT TATTCCGACT GGGTTTGAAG ACTCGGCCTG AATGCATGGA AACTCTGCAG 
AGATGTGGCT GGAATCTGGA ACAGGCCAGC ACCCAAATGC TGGATTCCTA TGGTCCATCT 
AGGCATAGGT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_009295760.1
CDS	249..3443
Translation	MQSDEGTEWLLELLTDVQLQQYFQRIRDELNVTRLSHFDYVKTEDLEKIGMGRPGQRRLWEAVKRRRAMCKRKSWMSKSLCLGQQVFTGKRPDSDSQTPEVFRSSSPSSAPPDGQPTDLTCLISERDLTLYEKLGDGSFGVVKRGEWQAPSGRVLSVAVKCLKTDLLDQGEGLDDFIREVNAMHSLNHHNLIRLYGCVLTHPMKMVTELAPLGSLLDRLRKRQGHILISVLCHYTVQIACGMAYLESRRFIHRDLAARNILLASNDLIKIGDFGLMRALPKNDDHYVMQEHHKVPFAWCAPESLKTRTFSHASDTWMFGVTLWEMFTHGQEPWVGLNGSQILHKIDREGERLIKPEDCPQDIYNVMLQCWSPKPEDRPTFVSLRDFLVETMPTDMRALQDFDEPDKLKIHTNDIITIIEGRAENYWWRGQNRQTMKVGQFPRNVVTSVAGLSAQDISRPLKHSFIHTGHGDTDPHRSWGAPDKIDDLYLGNPMDPPDVLGLDSNTAKPTKLPNRAKKQPPPRPPKPAVLLKKPFYDSVLDDDDDDDLIVTGVKKLSLKTPTYLGLRLRPWESTSPSDRLCEVSLIDFGDDSFSSTSPSPITEIPNPSTAKPPLSYAASILDMAPPQSPNQALPNPMHPTPVVDWDMRPLPPLPAYDEVAVECAEQEDIEVSSINASAAQEDTVYEQTPSEEDINNDGKRNVEDNLFLPSKRPEESHSFSQSADIFKELQREVMVKLRVPPSGRSLPSSPLPTPSVLTPHRQIILPSSYEDKPQIPPRIPIPPMRPSKRTGIYGSRLSVSLGENEDGSRCPPQIPPRDHALSQPNSRAPSPMVPQSGSPQQRSSLCCVGSLGSCLSPSSYSSAPSSSSTTSSTSTTTSFQYGSTEVSQNPAKSPCILPIVYGGVKASSTHYYLLPEKPAYLDKYEKFFNSEGNDEKRTTNMATVRPMVQQQGNKSNASPSHTSNSGTSSSLAWPSSSQAGSYSRVTLQQVQEAVHGVTVEECQTALQAHNWNAQEAVKYLKVEQLFRLGLKTRPECMETLQRCGWNLEQASTQMLDSYGPSRHRF

Target ORF information:

RefSeq Version	XM_009297485.2
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio activated CDC42 kinase 1-like (LOC100148041), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_009297485.2

ORF Insert Sequence:

ATGCAGTCTG ATGAAGGTAC AGAGTGGCTC TTGGAGCTGC TCACAGATGT GCAGCTTCAG 
CAATATTTCC AGCGTATCCG TGATGAGCTC AATGTCACGC GTCTCTCACA CTTTGACTAT 
GTCAAAACAG AGGACCTCGA AAAAATTGGC ATGGGCCGAC CAGGTCAAAG GCGGCTGTGG 
GAGGCAGTCA AAAGAAGAAG GGCAATGTGC AAACGGAAGT CATGGATGAG CAAGTCTCTG 
TGTTTGGGTC AGCAGGTGTT TACCGGGAAG CGCCCTGACT CTGATTCGCA GACTCCAGAG 
GTTTTCCGCA GCTCGTCCCC TTCATCGGCT CCTCCGGATG GCCAGCCGAC GGATCTCACC 
TGCCTCATCA GCGAACGAGA CCTCACTCTC TATGAAAAAC TGGGGGACGG ATCTTTCGGA 
GTGGTGAAGC GTGGAGAATG GCAGGCCCCC TCTGGAAGAG TGCTAAGCGT GGCTGTGAAG 
TGCCTGAAGA CGGACCTGTT GGACCAGGGT GAAGGTCTAG ATGACTTCAT ACGAGAAGTT 
AATGCCATGC ACTCCTTGAA CCACCACAAT CTCATTCGTC TGTATGGGTG CGTCCTCACA 
CACCCCATGA AGATGGTGAC AGAACTTGCT CCATTGGGTT CTCTGTTGGA CCGCCTGAGG 
AAACGTCAGG GTCATATACT GATATCAGTG CTGTGCCACT ACACTGTTCA GATCGCCTGT 
GGGATGGCAT ACCTGGAGTC TCGACGCTTC ATTCACAGAG ACCTGGCTGC ACGCAACATC 
CTTTTAGCCT CCAATGACCT CATCAAGATC GGTGACTTCG GCCTGATGAG GGCCTTGCCT 
AAAAATGATG ATCATTACGT TATGCAGGAG CATCACAAAG TCCCTTTTGC ATGGTGTGCT 
CCAGAGAGCC TGAAGACCCG TACCTTCTCC CATGCCAGTG ATACCTGGAT GTTTGGAGTA 
ACATTGTGGG AAATGTTCAC TCACGGGCAG GAACCATGGG TTGGACTCAA TGGTAGTCAG 
ATCCTCCATA AGATCGATAG AGAGGGAGAA AGGCTTATCA AACCAGAGGA CTGTCCTCAG 
GACATTTATA ACGTCATGCT GCAGTGCTGG TCACCCAAAC CTGAGGACAG GCCAACATTT 
GTGTCCCTTA GGGACTTCCT TGTGGAGACA ATGCCAACAG ATATGAGGGC ACTGCAGGAC 
TTTGATGAGC CAGACAAACT TAAAATCCAC ACTAATGACA TCATCACCAT CATTGAGGGC 
AGAGCAGAGA ACTACTGGTG GAGGGGTCAG AACAGACAGA CCATGAAGGT GGGACAGTTC 
CCCAGGAATG TAGTGACCTC TGTGGCCGGT CTATCTGCTC AGGACATCAG CCGACCTCTC 
AAGCACAGTT TCATCCACAC AGGCCATGGA GACACTGACC CACACCGTAG CTGGGGTGCC 
CCTGATAAGA TTGATGATCT GTACCTTGGT AATCCCATGG ATCCTCCAGA TGTTCTTGGG 
TTGGATTCAA ATACAGCCAA GCCCACTAAG CTACCAAACC GGGCTAAAAA ACAACCTCCT 
CCTAGACCTC CTAAACCAGC TGTTCTTCTT AAGAAACCAT TTTATGACTC TGTCCTGGAT 
GATGATGATG ATGATGATCT GATAGTGACA GGTGTGAAGA AGCTCTCACT TAAGACTCCT 
ACATACCTGG GTTTACGCCT TCGCCCATGG GAAAGCACTA GTCCGAGTGA CCGCCTTTGT 
GAAGTCTCCC TTATTGACTT TGGGGATGAT AGTTTCAGCT CAACATCGCC CTCTCCCATT 
ACCGAGATTC CCAATCCTAG CACAGCGAAA CCACCTTTGT CCTATGCCGC TTCCATCTTG 
GATATGGCAC CACCCCAGAG CCCAAATCAA GCCTTACCCA ACCCCATGCA CCCTACTCCA 
GTGGTAGACT GGGACATGCG GCCCCTGCCT CCTCTCCCGG CATATGATGA GGTGGCCGTT 
GAGTGTGCAG AGCAGGAAGA CATAGAAGTG AGCTCCATAA ATGCATCTGC AGCCCAGGAA 
GACACTGTAT ATGAACAGAC CCCATCAGAA GAAGACATTA ATAATGATGG TAAACGGAAC 
GTGGAGGATA ACCTCTTTCT TCCATCAAAG CGTCCTGAGG AATCCCACTC GTTTTCACAA 
TCAGCAGATA TCTTCAAGGA ACTGCAAAGA GAGGTGATGG TAAAGCTTCG GGTCCCTCCA 
AGTGGGCGTT CTCTACCTTC TTCACCCCTC CCGACTCCTT CAGTTCTCAC CCCACACCGT 
CAGATAATCC TTCCATCCTC TTATGAGGAC AAGCCTCAGA TCCCTCCAAG AATCCCTATT 
CCACCTATGC GGCCTTCAAA GAGGACAGGA ATATACGGCA GCAGACTGTC TGTTTCTCTT 
GGAGAAAATG AAGATGGGAG CCGTTGTCCA CCCCAAATAC CCCCGCGCGA TCATGCTCTA 
TCTCAGCCCA ATTCTCGGGC ACCAAGTCCT ATGGTGCCTC AAAGCGGCTC TCCTCAACAA 
AGATCCAGCC TGTGCTGTGT TGGATCATTG GGCTCCTGCT TGTCTCCATC ATCATATTCT 
TCTGCACCAT CCAGCTCGTC CACCACATCT TCCACATCCA CCACTACTAG TTTTCAATAT 
GGATCTACTG AAGTGAGCCA AAATCCAGCC AAGAGTCCTT GCATCCTGCC AATTGTTTAC 
GGTGGTGTAA AGGCCAGCAG CACTCATTAC TACTTGCTAC CTGAGAAACC GGCATACTTG 
GACAAGTATG AGAAGTTCTT TAATTCGGAG GGAAACGATG AGAAAAGGAC TACAAATATG 
GCTACTGTGA GACCCATGGT TCAGCAGCAA GGCAACAAAT CCAATGCTTC CCCAAGCCAC 
ACCAGTAACA GTGGGACCTC CAGCAGTCTG GCTTGGCCAA GCAGCTCCCA AGCAGGTTCT 
TACAGCCGTG TCACGCTGCA ACAAGTACAG GAAGCAGTAC ATGGGGTGAC TGTAGAGGAG 
TGTCAAACAG CCTTGCAGGC ACACAACTGG AATGCTCAGG AGGCCGTGAA GTATCTAAAG 
GTAGAACAGT TATTCCGACT GGGTTTGAAG ACTCGGCCTG AATGCATGGA AACTCTGCAG 
AGATGTGGCT GGAATCTGGA ACAGGCCAGC ACCCAAATGC TGGATTCCTA TGGTCCATCT 
AGGCATAGGT TCTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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LOC100148041 cDNA ORF clone, Danio rerio(zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

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