SEMA5BB cDNA ORF clone, Danio rerio(Zebrafish)

Gene
Clones
gRNAs

Summary

Gene Symbol	SEMA5BB
Entrez Gene ID	100000009
Full Name	sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5Bb
General protein information	Preferred Names sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5Bb Names semaphorin-5B zmp:0000000775
Gene Type	protein-coding
Organism	Danio rerio(Zebrafish)

mRNA and Protein(s)

mRNA	Protein	Name
XM_017356553.1	XP_017212042.1	semaphorin-5B isoform X1
XM_017356554.1	XP_017212043.1	semaphorin-5B isoform X2
XM_001340262.8	XP_001340298.5	semaphorin-5B
XM_017356553.2	XP_017212042.1	semaphorin-5B
XM_001340262.7	XP_001340298.5	semaphorin-5B isoform X1

Pathways from BioSystems
Gene Ontology

The following SEMA5BB gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SEMA5BB cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
ODa59411	XM_017356554.1 Latest version!	Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5Bb (sema5bb), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
ODa11406	XM_017356553.2 Latest version!	Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5Bb (sema5bb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa11406	XM_001340262.8 Latest version!	Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5Bb (sema5bb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
View Old Accession Versions >>
ODa11406	XM_017356553.1	Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5Bb (sema5bb), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
ODa11406	XM_001340262.7	Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5Bb (sema5bb), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$559.30 $799.00
Hide Old Accession Versions >>

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** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SEMA5BB ( XM_017356554.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017212043.1 Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5Bb (sema5bb), transcript variant X3, mRNA.

Price & Availability↑

CloneID	ODa59411
Clone ID Related Accession (Same CDS sequence)	XM_017356554.1
Accession Version	XM_017356554.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2778bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	semaphorin-5B isoform X2
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGA TGGTGTTCTG GGCCGGATCA CTGTCTTTCG TCGGACTGGT CATCTTTCAC 
CTCCTCTTTC TGCCTGTGGT CACGTCACAG ACACCATCAT CACCCCCAGA CACCCCTGAC 
CCGCAGCCTG AAGAGTGCCG GAGAAAAATG CACCCTGTTA CCTCCTACAC AGCGTTGAGT 
CCGTGGCTCT TCTCGTTCTC TGTGCCTGGA GTGAACGACT ACTCACCACT CACACTCGAC 
CTCAGCAGGA GCCAGCTGAT TGTGGGAGCC AGGAATCATC TCTTCCGGCT GAGTTTGAGT 
AACATCTCTC TTCTGCAGGC CACAGAATGG GGTGTTGACG AAACTACCAG GAGATCCTGC 
CAGAGCAAAG GAAAGACCGA GGACGAGTGC CAGAATTATG TGCGAGTCCT CCTGCTGAAC 
GGCAGTCGTT TGTTCACCTG CGGTACGAAC GCCTTCATGC CCGTCTGTGT GACGCGTCCC 
GTGACGGACA TCAGCAGCGT TCTGGACTCC ATCAGCGGTG TGGCTCGCTG TCCCTACGAT 
CCTCGACACA ACTCCACAGC CATGATCACA GAGAGCGGAG AGGTGTACGC CGCCACTGTG 
ACCGATTTCT CTAGCCGGGA CCCCATCATT TACCGCAGCC TGGGGAACAT GCCGCCTCTG 
CGCACCGCAC AGTACAACTC TAAATGGCTC AACGATCCTC ACTTCATCTC GGCGTATGAG 
GTGAGCCGTT TCACATACGT GTTTCTGCGA GAGAACGCAG TGGAGCAGGA TTGTGGGAAG 
ACGGTTTTCT CTCGCGTGGC TCGCGTGTGT CAGAACGATA TCGGCGGTCG CTTCCTTCTG 
GAAGACACGT GGACCACATT CATGAAGGCC CGGCTAAACT GCTCACGATC TGGAGACGTT 
CCCTTCTACT ACCATGAGCT GCAGAGCACT TTCTATTTGC CCGAACAAGA CCTCATCTAC 
GGGGTCTTCA CCACCAATGT GAACAGTATT GCTGCGTCAG CCGTGTGTGC GTTTAACCTC 
AGTGCCATCA CTCAGGCCTT TAACGGGCCT TTCCGCTATC AGGAGAACCC TCGCACCAGC 
TGGCTGTCCA CTCCAAACCC CGTCCCAAAC TTCCAGTGCG GGACGGTGAA TGATTCGGGG 
CCGGGTGCTA ATCTGACAGA GCGCAGTCTT CAAGACGCCC AGCGGCTCTT TCTGATGAGC 
GACGTGGTTC AGCCCATCTC CACAGACCCG CTGGTCATGC AGGACAACAT CCGCTTCTCC 
AAACTGGCGG TGGACATCGT TCAGGGCAGA GACACGCTTT ACCACGTCAT GTACATCGGC 
ACAGAATATG GCACCATCAT CAAGGCCTTG TCCACTAATA ATAAGAGTCT GCGTGGATGT 
TATTTGGAGG AGATGAACAT TCTGCCGGGA AACAAGCAGG AGCCCATCCT GAACCTGCAG 
ATTCTGCACA GCGATAGATC TCTGTTTGTG GGGCTTCCCA GCAGAGTGTT GAAGATCCCT 
TTGGAAAGAT GCTCTAACTA CATCACTGAA CAGGATTGTC TGGGTGCCAG GGATCCGTAC 
TGCGGCTGGG ACCGTAAAGA GAAGCGCTGC ACCACCATTG AGGACAGCTC CAACATGAGC 
CAATGGAGCC AGGACATCAC AAAGTGCCCT GAGAGGAACC TCACTCAAGA TGGCGGCTTT 
AGTGATTGGT CAACATGGCA GGCTTGTAAC CATGATGACG GAGGGGAAGG CATCAGCACA 
TGCCAGTGTC GCACCCGGGC CTGCGATCAC CCTCGACCCC AGTGTGGAGG ACTAAAGTGT 
GTCGGGGCTA ATATAGAGGT GGCCAACTGC TCCAGGAATG GAGGCTGGAC ACCCTGGTCA 
TCCTGGGCCG AGTGCAGCAC CAGCTGTGGG ATTGGGTTTG AGGTCCGTCA GCGATCCTGT 
AACAATCCTT CACCTCGACA CGGAGGACGC GTGTGTGTAG GCCAGGCCCG AGAGGAGAGA 
CTGTGTAATG AGAAGAATCC ATGTCCTGTC CCGATGTCCT GGGTCTCCTG GAGCGCCTGG 
TCCAAATGTA GCGTGGCCTG CGGTGGTGGA GTCCAATCCC GCGTTCGAAC CTGCGAGAAT 
GGCAACACGT GTCCTGGATG CCCTCTGGAG TACAGAGCGT GTAATTTGGA CTCGTGTGCT 
GAAGTGAGGC GAACGACACC GTGGACCCCC TGGTATCCTG TTAATGTGAC CCAGCTGGGA 
GCGCGGCAGG AGCAGAGGGT CCGATATACC TGTAGAGCTC TGCTGGCAGA CCCTCATGAC 
TTACAGCTCG GCAAACGCAA GATAGAGACA CGCCTCTGTC CCACCACTGA CGGAGCTGCT 
GCCTGCGAGA CTGATGGTCT GGTTGAGGAT TTGTTGAGAA TGGGTCGACC TGTAACACGA 
GTGCAAGGAG CCGCCTGGGC ATTATGGGAA ACCTGGTCTA CATGTTCAAA AGAATGTTCC 
AAAGGCTTCC GCACACGCAA ACGCTCCTGT GCCGCACCGG ATGGCAAGAG CACACCGTTC 
GCCTGCAGCG GAGCCCCGGT CGAGTATCAG GACTGCAACA CTCAACCCTG CCCAGTGAAG 
GGGGCGTGGT CATGCTGGTC TTCCTGGTCT CAGTGTTCTA CTTCCTGTGG TGGCGGTCAT 
CACCAGCGCT CCCGTTCCTG CTCGAACCCT GCGCCGGCTT ATTCTGGAGA CATCTGCATC 
GGCCTGCACA CAGAGGAGGC GCTCTGTAAC ACACACGAGT GTGAAGGTGA GGAAACACAC 
AACAGTGATG AAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212043.1
CDS	222..2999
Translation	MAAMVFWAGSLSFVGLVIFHLLFLPVVTSQTPSSPPDTPDPQPEECRRKMHPVTSYTALSPWLFSFSVPGVNDYSPLTLDLSRSQLIVGARNHLFRLSLSNISLLQATEWGVDETTRRSCQSKGKTEDECQNYVRVLLLNGSRLFTCGTNAFMPVCVTRPVTDISSVLDSISGVARCPYDPRHNSTAMITESGEVYAATVTDFSSRDPIIYRSLGNMPPLRTAQYNSKWLNDPHFISAYEVSRFTYVFLRENAVEQDCGKTVFSRVARVCQNDIGGRFLLEDTWTTFMKARLNCSRSGDVPFYYHELQSTFYLPEQDLIYGVFTTNVNSIAASAVCAFNLSAITQAFNGPFRYQENPRTSWLSTPNPVPNFQCGTVNDSGPGANLTERSLQDAQRLFLMSDVVQPISTDPLVMQDNIRFSKLAVDIVQGRDTLYHVMYIGTEYGTIIKALSTNNKSLRGCYLEEMNILPGNKQEPILNLQILHSDRSLFVGLPSRVLKIPLERCSNYITEQDCLGARDPYCGWDRKEKRCTTIEDSSNMSQWSQDITKCPERNLTQDGGFSDWSTWQACNHDDGGEGISTCQCRTRACDHPRPQCGGLKCVGANIEVANCSRNGGWTPWSSWAECSTSCGIGFEVRQRSCNNPSPRHGGRVCVGQAREERLCNEKNPCPVPMSWVSWSAWSKCSVACGGGVQSRVRTCENGNTCPGCPLEYRACNLDSCAEVRRTTPWTPWYPVNVTQLGARQEQRVRYTCRALLADPHDLQLGKRKIETRLCPTTDGAAACETDGLVEDLLRMGRPVTRVQGAAWALWETWSTCSKECSKGFRTRKRSCAAPDGKSTPFACSGAPVEYQDCNTQPCPVKGAWSCWSSWSQCSTSCGGGHHQRSRSCSNPAPAYSGDICIGLHTEEALCNTHECEGEETHNSDEI

Target ORF information:

RefSeq Version	XM_017356554.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5Bb (sema5bb), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356554.1

ORF Insert Sequence:

ATGGCAGCGA TGGTGTTCTG GGCCGGATCA CTGTCTTTCG TCGGACTGGT CATCTTTCAC 
CTCCTCTTTC TGCCTGTGGT CACGTCACAG ACACCATCAT CACCCCCAGA CACCCCTGAC 
CCGCAGCCTG AAGAGTGCCG GAGAAAAATG CACCCTGTTA CCTCCTACAC AGCGTTGAGT 
CCGTGGCTCT TCTCGTTCTC TGTGCCTGGA GTGAACGACT ACTCACCACT CACACTCGAC 
CTCAGCAGGA GCCAGCTGAT TGTGGGAGCC AGGAATCATC TCTTCCGGCT GAGTTTGAGT 
AACATCTCTC TTCTGCAGGC CACAGAATGG GGTGTTGACG AAACTACCAG GAGATCCTGC 
CAGAGCAAAG GAAAGACCGA GGACGAGTGC CAGAATTATG TGCGAGTCCT CCTGCTGAAC 
GGCAGTCGTT TGTTCACCTG CGGTACGAAC GCCTTCATGC CCGTCTGTGT GACGCGTCCC 
GTGACGGACA TCAGCAGCGT TCTGGACTCC ATCAGCGGTG TGGCTCGCTG TCCCTACGAT 
CCTCGACACA ACTCCACAGC CATGATCACA GAGAGCGGAG AGGTGTACGC CGCCACTGTG 
ACCGATTTCT CTAGCCGGGA CCCCATCATT TACCGCAGCC TGGGGAACAT GCCGCCTCTG 
CGCACCGCAC AGTACAACTC TAAATGGCTC AACGATCCTC ACTTCATCTC GGCGTATGAG 
GTGAGCCGTT TCACATACGT GTTTCTGCGA GAGAACGCAG TGGAGCAGGA TTGTGGGAAG 
ACGGTTTTCT CTCGCGTGGC TCGCGTGTGT CAGAACGATA TCGGCGGTCG CTTCCTTCTG 
GAAGACACGT GGACCACATT CATGAAGGCC CGGCTAAACT GCTCACGATC TGGAGACGTT 
CCCTTCTACT ACCATGAGCT GCAGAGCACT TTCTATTTGC CCGAACAAGA CCTCATCTAC 
GGGGTCTTCA CCACCAATGT GAACAGTATT GCTGCGTCAG CCGTGTGTGC GTTTAACCTC 
AGTGCCATCA CTCAGGCCTT TAACGGGCCT TTCCGCTATC AGGAGAACCC TCGCACCAGC 
TGGCTGTCCA CTCCAAACCC CGTCCCAAAC TTCCAGTGCG GGACGGTGAA TGATTCGGGG 
CCGGGTGCTA ATCTGACAGA GCGCAGTCTT CAAGACGCCC AGCGGCTCTT TCTGATGAGC 
GACGTGGTTC AGCCCATCTC CACAGACCCG CTGGTCATGC AGGACAACAT CCGCTTCTCC 
AAACTGGCGG TGGACATCGT TCAGGGCAGA GACACGCTTT ACCACGTCAT GTACATCGGC 
ACAGAATATG GCACCATCAT CAAGGCCTTG TCCACTAATA ATAAGAGTCT GCGTGGATGT 
TATTTGGAGG AGATGAACAT TCTGCCGGGA AACAAGCAGG AGCCCATCCT GAACCTGCAG 
ATTCTGCACA GCGATAGATC TCTGTTTGTG GGGCTTCCCA GCAGAGTGTT GAAGATCCCT 
TTGGAAAGAT GCTCTAACTA CATCACTGAA CAGGATTGTC TGGGTGCCAG GGATCCGTAC 
TGCGGCTGGG ACCGTAAAGA GAAGCGCTGC ACCACCATTG AGGACAGCTC CAACATGAGC 
CAATGGAGCC AGGACATCAC AAAGTGCCCT GAGAGGAACC TCACTCAAGA TGGCGGCTTT 
AGTGATTGGT CAACATGGCA GGCTTGTAAC CATGATGACG GAGGGGAAGG CATCAGCACA 
TGCCAGTGTC GCACCCGGGC CTGCGATCAC CCTCGACCCC AGTGTGGAGG ACTAAAGTGT 
GTCGGGGCTA ATATAGAGGT GGCCAACTGC TCCAGGAATG GAGGCTGGAC ACCCTGGTCA 
TCCTGGGCCG AGTGCAGCAC CAGCTGTGGG ATTGGGTTTG AGGTCCGTCA GCGATCCTGT 
AACAATCCTT CACCTCGACA CGGAGGACGC GTGTGTGTAG GCCAGGCCCG AGAGGAGAGA 
CTGTGTAATG AGAAGAATCC ATGTCCTGTC CCGATGTCCT GGGTCTCCTG GAGCGCCTGG 
TCCAAATGTA GCGTGGCCTG CGGTGGTGGA GTCCAATCCC GCGTTCGAAC CTGCGAGAAT 
GGCAACACGT GTCCTGGATG CCCTCTGGAG TACAGAGCGT GTAATTTGGA CTCGTGTGCT 
GAAGTGAGGC GAACGACACC GTGGACCCCC TGGTATCCTG TTAATGTGAC CCAGCTGGGA 
GCGCGGCAGG AGCAGAGGGT CCGATATACC TGTAGAGCTC TGCTGGCAGA CCCTCATGAC 
TTACAGCTCG GCAAACGCAA GATAGAGACA CGCCTCTGTC CCACCACTGA CGGAGCTGCT 
GCCTGCGAGA CTGATGGTCT GGTTGAGGAT TTGTTGAGAA TGGGTCGACC TGTAACACGA 
GTGCAAGGAG CCGCCTGGGC ATTATGGGAA ACCTGGTCTA CATGTTCAAA AGAATGTTCC 
AAAGGCTTCC GCACACGCAA ACGCTCCTGT GCCGCACCGG ATGGCAAGAG CACACCGTTC 
GCCTGCAGCG GAGCCCCGGT CGAGTATCAG GACTGCAACA CTCAACCCTG CCCAGTGAAG 
GGGGCGTGGT CATGCTGGTC TTCCTGGTCT CAGTGTTCTA CTTCCTGTGG TGGCGGTCAT 
CACCAGCGCT CCCGTTCCTG CTCGAACCCT GCGCCGGCTT ATTCTGGAGA CATCTGCATC 
GGCCTGCACA CAGAGGAGGC GCTCTGTAAC ACACACGAGT GTGAAGGTGA GGAAACACAC 
AACAGTGATG AAATTTAA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA5BB ( XM_017356553.1 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017212042.1 Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5Bb (sema5bb), transcript variant X2, mRNA.

Price & Availability↑

CloneID	ODa11406
Clone ID Related Accession (Same CDS sequence)	XM_017356553.1 , XM_001340262.8 , XM_017356553.2 , XM_001340262.7
Accession Version	XM_017356553.1	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	semaphorin-5B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGA TGGTGTTCTG GGCCGGATCA CTGTCTTTCG TCGGACTGGT CATCTTTCAC 
CTCCTCTTTC TGCCTGTGGT CACGTCACAG ACACCATCAT CACCCCCAGA CACCCCTGAC 
CCGCAGCCTG AAGAGTGCCG GAGAAAAATG CACCCTGTTA CCTCCTACAC AGCGTTGAGT 
CCGTGGCTCT TCTCGTTCTC TGTGCCTGGA GTGAACGACT ACTCACCACT CACACTCGAC 
CTCAGCAGGA GCCAGCTGAT TGTGGGAGCC AGGAATCATC TCTTCCGGCT GAGTTTGAGT 
AACATCTCTC TTCTGCAGGC CACAGAATGG GGTGTTGACG AAACTACCAG GAGATCCTGC 
CAGAGCAAAG GAAAGACCGA GGACGAGTGC CAGAATTATG TGCGAGTCCT CCTGCTGAAC 
GGCAGTCGTT TGTTCACCTG CGGTACGAAC GCCTTCATGC CCGTCTGTGT GACGCGTCCC 
GTGACGGACA TCAGCAGCGT TCTGGACTCC ATCAGCGGTG TGGCTCGCTG TCCCTACGAT 
CCTCGACACA ACTCCACAGC CATGATCACA GAGAGCGGAG AGGTGTACGC CGCCACTGTG 
ACCGATTTCT CTAGCCGGGA CCCCATCATT TACCGCAGCC TGGGGAACAT GCCGCCTCTG 
CGCACCGCAC AGTACAACTC TAAATGGCTC AACGATCCTC ACTTCATCTC GGCGTATGAG 
GTGAGCCGTT TCACATACGT GTTTCTGCGA GAGAACGCAG TGGAGCAGGA TTGTGGGAAG 
ACGGTTTTCT CTCGCGTGGC TCGCGTGTGT CAGAACGATA TCGGCGGTCG CTTCCTTCTG 
GAAGACACGT GGACCACATT CATGAAGGCC CGGCTAAACT GCTCACGATC TGGAGACGTT 
CCCTTCTACT ACCATGAGCT GCAGAGCACT TTCTATTTGC CCGAACAAGA CCTCATCTAC 
GGGGTCTTCA CCACCAATGT GAACAGTATT GCTGCGTCAG CCGTGTGTGC GTTTAACCTC 
AGTGCCATCA CTCAGGCCTT TAACGGGCCT TTCCGCTATC AGGAGAACCC TCGCACCAGC 
TGGCTGTCCA CTCCAAACCC CGTCCCAAAC TTCCAGTGCG GGACGGTGAA TGATTCGGGG 
CCGGGTGCTA ATCTGACAGA GCGCAGTCTT CAAGACGCCC AGCGGCTCTT TCTGATGAGC 
GACGTGGTTC AGCCCATCTC CACAGACCCG CTGGTCATGC AGGACAACAT CCGCTTCTCC 
AAACTGGCGG TGGACATCGT TCAGGGCAGA GACACGCTTT ACCACGTCAT GTACATCGGC 
ACAGAATATG GCACCATCAT CAAGGCCTTG TCCACTAATA ATAAGAGTCT GCGTGGATGT 
TATTTGGAGG AGATGAACAT TCTGCCGGGA AACAAGCAGG AGCCCATCCT GAACCTGCAG 
ATTCTGCACA GCGATAGATC TCTGTTTGTG GGGCTTCCCA GCAGAGTGTT GAAGATCCCT 
TTGGAAAGAT GCTCTAACTA CATCACTGAA CAGGATTGTC TGGGTGCCAG GGATCCGTAC 
TGCGGCTGGG ACCGTAAAGA GAAGCGCTGC ACCACCATTG AGGACAGCTC CAACATGAGC 
CAATGGAGCC AGGACATCAC AAAGTGCCCT GAGAGGAACC TCACTCAAGA TGGCGGCTTT 
AGTGATTGGT CAACATGGCA GGCTTGTAAC CATGATGACG GAGGGGAAGG CATCAGCACA 
TGCCAGTGTC GCACCCGGGC CTGCGATCAC CCTCGACCCC AGTGTGGAGG ACTAAAGTGT 
GTCGGGGCTA ATATAGAGGT GGCCAACTGC TCCAGGAATG GAGGCTGGAC ACCCTGGTCA 
TCCTGGGCCG AGTGCAGCAC CAGCTGTGGG ATTGGGTTTG AGGTCCGTCA GCGATCCTGT 
AACAATCCTT CACCTCGACA CGGAGGACGC GTGTGTGTAG GCCAGGCCCG AGAGGAGAGA 
CTGTGTAATG AGAAGAATCC ATGTCCTGTC CCGATGTCCT GGGTCTCCTG GAGCGCCTGG 
TCCAAATGTA GCGTGGCCTG CGGTGGTGGA GTCCAATCCC GCGTTCGAAC CTGCGAGAAT 
GGCAACACGT GTCCTGGATG CCCTCTGGAG TACAGAGCGT GTAATTTGGA CTCGTGTGCT 
GAAGTGAGGC GAACGACACC GTGGACCCCC TGGTATCCTG TTAATGTGAC CCAGCTGGGA 
GCGCGGCAGG AGCAGAGGGT CCGATATACC TGTAGAGCTC TGCTGGCAGA CCCTCATGAC 
TTACAGCTCG GCAAACGCAA GATAGAGACA CGCCTCTGTC CCACCACTGA CGGAGCTGCT 
GCCTGCGAGA CTGATGGTCT GGTTGAGGAT TTGTTGAGAA TGGGTCGACC TGTAACACGA 
GTGCAAGGAG CCGCCTGGGC ATTATGGGAA ACCTGGTCTA CATGTTCAAA AGAATGTTCC 
AAAGGCTTCC GCACACGCAA ACGCTCCTGT GCCGCACCGG ATGGCAAGAG CACACCGTTC 
GCCTGCAGCG GAGCCCCGGT CGAGTATCAG GACTGCAACA CTCAACCCTG CCCAGTGAAG 
GGGGCGTGGT CATGCTGGTC TTCCTGGTCT CAGTGTTCTA CTTCCTGTGG TGGCGGTCAT 
CACCAGCGCT CCCGTTCCTG CTCGAACCCT GCGCCGGCTT ATTCTGGAGA CATCTGCATC 
GGCCTGCACA CAGAGGAGGC GCTCTGTAAC ACACACGAGT GTGAAGGTGG GTGGGGGGCG 
TGGTCAGAGT GGGCAGAGTG TAATGGGGAG GGGCTTCAGC TCCGGGCTCG TACATGTGAA 
ATAAGCCCCG CCTCCTGTCT TGGAAACTCC ACAGATCAGA GACAGTGTCG GCCCAATGAG 
AGCGCAGTGC TGTCAAAAGG ACAGAAGAGG AGCACAAACT GTGGAGGGTT TTCTCTGTTC 
CACCTGGTTC TGACTGGTGC ATGTTGTTTT CTGGGAGCCG TTTTACTCTC CATCCTGGTC 
TATGTGTACT GTCAGAGACT CCGCAAACCT CCGCAAGAAT CTGCAGTCAT CCATCCCAGC 
ACCCCAAACC ATCTCCACTA TAAAGACAAC AGCTGCGCGC CCAAGAACGA TTTATACACA 
CCCATGGAGT TCAAGACTCT CAATAAGAAC AACCTGCTTC CACCAGATGA AAGCTGCAAC 
TTCTTCCCAT CGCCCTTACC ACAGACCAAC GTCTACACCA CCACATATTA CCCGTCTCCG 
CTGGGCAAAT ACGACTTCCC TTCAATGGAT TCCCCCTGCA GCTTCATGCA CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212042.1
CDS	181..3477
Translation	MAAMVFWAGSLSFVGLVIFHLLFLPVVTSQTPSSPPDTPDPQPEECRRKMHPVTSYTALSPWLFSFSVPGVNDYSPLTLDLSRSQLIVGARNHLFRLSLSNISLLQATEWGVDETTRRSCQSKGKTEDECQNYVRVLLLNGSRLFTCGTNAFMPVCVTRPVTDISSVLDSISGVARCPYDPRHNSTAMITESGEVYAATVTDFSSRDPIIYRSLGNMPPLRTAQYNSKWLNDPHFISAYEVSRFTYVFLRENAVEQDCGKTVFSRVARVCQNDIGGRFLLEDTWTTFMKARLNCSRSGDVPFYYHELQSTFYLPEQDLIYGVFTTNVNSIAASAVCAFNLSAITQAFNGPFRYQENPRTSWLSTPNPVPNFQCGTVNDSGPGANLTERSLQDAQRLFLMSDVVQPISTDPLVMQDNIRFSKLAVDIVQGRDTLYHVMYIGTEYGTIIKALSTNNKSLRGCYLEEMNILPGNKQEPILNLQILHSDRSLFVGLPSRVLKIPLERCSNYITEQDCLGARDPYCGWDRKEKRCTTIEDSSNMSQWSQDITKCPERNLTQDGGFSDWSTWQACNHDDGGEGISTCQCRTRACDHPRPQCGGLKCVGANIEVANCSRNGGWTPWSSWAECSTSCGIGFEVRQRSCNNPSPRHGGRVCVGQAREERLCNEKNPCPVPMSWVSWSAWSKCSVACGGGVQSRVRTCENGNTCPGCPLEYRACNLDSCAEVRRTTPWTPWYPVNVTQLGARQEQRVRYTCRALLADPHDLQLGKRKIETRLCPTTDGAAACETDGLVEDLLRMGRPVTRVQGAAWALWETWSTCSKECSKGFRTRKRSCAAPDGKSTPFACSGAPVEYQDCNTQPCPVKGAWSCWSSWSQCSTSCGGGHHQRSRSCSNPAPAYSGDICIGLHTEEALCNTHECEGGWGAWSEWAECNGEGLQLRARTCEISPASCLGNSTDQRQCRPNESAVLSKGQKRSTNCGGFSLFHLVLTGACCFLGAVLLSILVYVYCQRLRKPPQESAVIHPSTPNHLHYKDNSCAPKNDLYTPMEFKTLNKNNLLPPDESCNFFPSPLPQTNVYTTTYYPSPLGKYDFPSMDSPCSFMHS

Target ORF information:

RefSeq Version	XM_017356553.1
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5Bb (sema5bb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356553.1

ORF Insert Sequence:

ATGGCAGCGA TGGTGTTCTG GGCCGGATCA CTGTCTTTCG TCGGACTGGT CATCTTTCAC 
CTCCTCTTTC TGCCTGTGGT CACGTCACAG ACACCATCAT CACCCCCAGA CACCCCTGAC 
CCGCAGCCTG AAGAGTGCCG GAGAAAAATG CACCCTGTTA CCTCCTACAC AGCGTTGAGT 
CCGTGGCTCT TCTCGTTCTC TGTGCCTGGA GTGAACGACT ACTCACCACT CACACTCGAC 
CTCAGCAGGA GCCAGCTGAT TGTGGGAGCC AGGAATCATC TCTTCCGGCT GAGTTTGAGT 
AACATCTCTC TTCTGCAGGC CACAGAATGG GGTGTTGACG AAACTACCAG GAGATCCTGC 
CAGAGCAAAG GAAAGACCGA GGACGAGTGC CAGAATTATG TGCGAGTCCT CCTGCTGAAC 
GGCAGTCGTT TGTTCACCTG CGGTACGAAC GCCTTCATGC CCGTCTGTGT GACGCGTCCC 
GTGACGGACA TCAGCAGCGT TCTGGACTCC ATCAGCGGTG TGGCTCGCTG TCCCTACGAT 
CCTCGACACA ACTCCACAGC CATGATCACA GAGAGCGGAG AGGTGTACGC CGCCACTGTG 
ACCGATTTCT CTAGCCGGGA CCCCATCATT TACCGCAGCC TGGGGAACAT GCCGCCTCTG 
CGCACCGCAC AGTACAACTC TAAATGGCTC AACGATCCTC ACTTCATCTC GGCGTATGAG 
GTGAGCCGTT TCACATACGT GTTTCTGCGA GAGAACGCAG TGGAGCAGGA TTGTGGGAAG 
ACGGTTTTCT CTCGCGTGGC TCGCGTGTGT CAGAACGATA TCGGCGGTCG CTTCCTTCTG 
GAAGACACGT GGACCACATT CATGAAGGCC CGGCTAAACT GCTCACGATC TGGAGACGTT 
CCCTTCTACT ACCATGAGCT GCAGAGCACT TTCTATTTGC CCGAACAAGA CCTCATCTAC 
GGGGTCTTCA CCACCAATGT GAACAGTATT GCTGCGTCAG CCGTGTGTGC GTTTAACCTC 
AGTGCCATCA CTCAGGCCTT TAACGGGCCT TTCCGCTATC AGGAGAACCC TCGCACCAGC 
TGGCTGTCCA CTCCAAACCC CGTCCCAAAC TTCCAGTGCG GGACGGTGAA TGATTCGGGG 
CCGGGTGCTA ATCTGACAGA GCGCAGTCTT CAAGACGCCC AGCGGCTCTT TCTGATGAGC 
GACGTGGTTC AGCCCATCTC CACAGACCCG CTGGTCATGC AGGACAACAT CCGCTTCTCC 
AAACTGGCGG TGGACATCGT TCAGGGCAGA GACACGCTTT ACCACGTCAT GTACATCGGC 
ACAGAATATG GCACCATCAT CAAGGCCTTG TCCACTAATA ATAAGAGTCT GCGTGGATGT 
TATTTGGAGG AGATGAACAT TCTGCCGGGA AACAAGCAGG AGCCCATCCT GAACCTGCAG 
ATTCTGCACA GCGATAGATC TCTGTTTGTG GGGCTTCCCA GCAGAGTGTT GAAGATCCCT 
TTGGAAAGAT GCTCTAACTA CATCACTGAA CAGGATTGTC TGGGTGCCAG GGATCCGTAC 
TGCGGCTGGG ACCGTAAAGA GAAGCGCTGC ACCACCATTG AGGACAGCTC CAACATGAGC 
CAATGGAGCC AGGACATCAC AAAGTGCCCT GAGAGGAACC TCACTCAAGA TGGCGGCTTT 
AGTGATTGGT CAACATGGCA GGCTTGTAAC CATGATGACG GAGGGGAAGG CATCAGCACA 
TGCCAGTGTC GCACCCGGGC CTGCGATCAC CCTCGACCCC AGTGTGGAGG ACTAAAGTGT 
GTCGGGGCTA ATATAGAGGT GGCCAACTGC TCCAGGAATG GAGGCTGGAC ACCCTGGTCA 
TCCTGGGCCG AGTGCAGCAC CAGCTGTGGG ATTGGGTTTG AGGTCCGTCA GCGATCCTGT 
AACAATCCTT CACCTCGACA CGGAGGACGC GTGTGTGTAG GCCAGGCCCG AGAGGAGAGA 
CTGTGTAATG AGAAGAATCC ATGTCCTGTC CCGATGTCCT GGGTCTCCTG GAGCGCCTGG 
TCCAAATGTA GCGTGGCCTG CGGTGGTGGA GTCCAATCCC GCGTTCGAAC CTGCGAGAAT 
GGCAACACGT GTCCTGGATG CCCTCTGGAG TACAGAGCGT GTAATTTGGA CTCGTGTGCT 
GAAGTGAGGC GAACGACACC GTGGACCCCC TGGTATCCTG TTAATGTGAC CCAGCTGGGA 
GCGCGGCAGG AGCAGAGGGT CCGATATACC TGTAGAGCTC TGCTGGCAGA CCCTCATGAC 
TTACAGCTCG GCAAACGCAA GATAGAGACA CGCCTCTGTC CCACCACTGA CGGAGCTGCT 
GCCTGCGAGA CTGATGGTCT GGTTGAGGAT TTGTTGAGAA TGGGTCGACC TGTAACACGA 
GTGCAAGGAG CCGCCTGGGC ATTATGGGAA ACCTGGTCTA CATGTTCAAA AGAATGTTCC 
AAAGGCTTCC GCACACGCAA ACGCTCCTGT GCCGCACCGG ATGGCAAGAG CACACCGTTC 
GCCTGCAGCG GAGCCCCGGT CGAGTATCAG GACTGCAACA CTCAACCCTG CCCAGTGAAG 
GGGGCGTGGT CATGCTGGTC TTCCTGGTCT CAGTGTTCTA CTTCCTGTGG TGGCGGTCAT 
CACCAGCGCT CCCGTTCCTG CTCGAACCCT GCGCCGGCTT ATTCTGGAGA CATCTGCATC 
GGCCTGCACA CAGAGGAGGC GCTCTGTAAC ACACACGAGT GTGAAGGTGG GTGGGGGGCG 
TGGTCAGAGT GGGCAGAGTG TAATGGGGAG GGGCTTCAGC TCCGGGCTCG TACATGTGAA 
ATAAGCCCCG CCTCCTGTCT TGGAAACTCC ACAGATCAGA GACAGTGTCG GCCCAATGAG 
AGCGCAGTGC TGTCAAAAGG ACAGAAGAGG AGCACAAACT GTGGAGGGTT TTCTCTGTTC 
CACCTGGTTC TGACTGGTGC ATGTTGTTTT CTGGGAGCCG TTTTACTCTC CATCCTGGTC 
TATGTGTACT GTCAGAGACT CCGCAAACCT CCGCAAGAAT CTGCAGTCAT CCATCCCAGC 
ACCCCAAACC ATCTCCACTA TAAAGACAAC AGCTGCGCGC CCAAGAACGA TTTATACACA 
CCCATGGAGT TCAAGACTCT CAATAAGAAC AACCTGCTTC CACCAGATGA AAGCTGCAAC 
TTCTTCCCAT CGCCCTTACC ACAGACCAAC GTCTACACCA CCACATATTA CCCGTCTCCG 
CTGGGCAAAT ACGACTTCCC TTCAATGGAT TCCCCCTGCA GCTTCATGCA CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA5BB ( XM_001340262.8 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_001340298.5 Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5Bb (sema5bb), transcript variant X1, mRNA.

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CloneID	ODa11406
Clone ID Related Accession (Same CDS sequence)	XM_017356553.1 , XM_001340262.8 , XM_017356553.2 , XM_001340262.7
Accession Version	XM_001340262.8 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	semaphorin-5B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_001340262.7. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGA TGGTGTTCTG GGCCGGATCA CTGTCTTTCG TCGGACTGGT CATCTTTCAC 
CTCCTCTTTC TGCCTGTGGT CACGTCACAG ACACCATCAT CACCCCCAGA CACCCCTGAC 
CCGCAGCCTG AAGAGTGCCG GAGAAAAATG CACCCTGTTA CCTCCTACAC AGCGTTGAGT 
CCGTGGCTCT TCTCGTTCTC TGTGCCTGGA GTGAACGACT ACTCACCACT CACACTCGAC 
CTCAGCAGGA GCCAGCTGAT TGTGGGAGCC AGGAATCATC TCTTCCGGCT GAGTTTGAGT 
AACATCTCTC TTCTGCAGGC CACAGAATGG GGTGTTGACG AAACTACCAG GAGATCCTGC 
CAGAGCAAAG GAAAGACCGA GGACGAGTGC CAGAATTATG TGCGAGTCCT CCTGCTGAAC 
GGCAGTCGTT TGTTCACCTG CGGTACGAAC GCCTTCATGC CCGTCTGTGT GACGCGTCCC 
GTGACGGACA TCAGCAGCGT TCTGGACTCC ATCAGCGGTG TGGCTCGCTG TCCCTACGAT 
CCTCGACACA ACTCCACAGC CATGATCACA GAGAGCGGAG AGGTGTACGC CGCCACTGTG 
ACCGATTTCT CTAGCCGGGA CCCCATCATT TACCGCAGCC TGGGGAACAT GCCGCCTCTG 
CGCACCGCAC AGTACAACTC TAAATGGCTC AACGATCCTC ACTTCATCTC GGCGTATGAG 
GTGAGCCGTT TCACATACGT GTTTCTGCGA GAGAACGCAG TGGAGCAGGA TTGTGGGAAG 
ACGGTTTTCT CTCGCGTGGC TCGCGTGTGT CAGAACGATA TCGGCGGTCG CTTCCTTCTG 
GAAGACACGT GGACCACATT CATGAAGGCC CGGCTAAACT GCTCACGATC TGGAGACGTT 
CCCTTCTACT ACCATGAGCT GCAGAGCACT TTCTATTTGC CCGAACAAGA CCTCATCTAC 
GGGGTCTTCA CCACCAATGT GAACAGTATT GCTGCGTCAG CCGTGTGTGC GTTTAACCTC 
AGTGCCATCA CTCAGGCCTT TAACGGGCCT TTCCGCTATC AGGAGAACCC TCGCACCAGC 
TGGCTGTCCA CTCCAAACCC CGTCCCAAAC TTCCAGTGCG GGACGGTGAA TGATTCGGGG 
CCGGGTGCTA ATCTGACAGA GCGCAGTCTT CAAGACGCCC AGCGGCTCTT TCTGATGAGC 
GACGTGGTTC AGCCCATCTC CACAGACCCG CTGGTCATGC AGGACAACAT CCGCTTCTCC 
AAACTGGCGG TGGACATCGT TCAGGGCAGA GACACGCTTT ACCACGTCAT GTACATCGGC 
ACAGAATATG GCACCATCAT CAAGGCCTTG TCCACTAATA ATAAGAGTCT GCGTGGATGT 
TATTTGGAGG AGATGAACAT TCTGCCGGGA AACAAGCAGG AGCCCATCCT GAACCTGCAG 
ATTCTGCACA GCGATAGATC TCTGTTTGTG GGGCTTCCCA GCAGAGTGTT GAAGATCCCT 
TTGGAAAGAT GCTCTAACTA CATCACTGAA CAGGATTGTC TGGGTGCCAG GGATCCGTAC 
TGCGGCTGGG ACCGTAAAGA GAAGCGCTGC ACCACCATTG AGGACAGCTC CAACATGAGC 
CAATGGAGCC AGGACATCAC AAAGTGCCCT GAGAGGAACC TCACTCAAGA TGGCGGCTTT 
AGTGATTGGT CAACATGGCA GGCTTGTAAC CATGATGACG GAGGGGAAGG CATCAGCACA 
TGCCAGTGTC GCACCCGGGC CTGCGATCAC CCTCGACCCC AGTGTGGAGG ACTAAAGTGT 
GTCGGGGCTA ATATAGAGGT GGCCAACTGC TCCAGGAATG GAGGCTGGAC ACCCTGGTCA 
TCCTGGGCCG AGTGCAGCAC CAGCTGTGGG ATTGGGTTTG AGGTCCGTCA GCGATCCTGT 
AACAATCCTT CACCTCGACA CGGAGGACGC GTGTGTGTAG GCCAGGCCCG AGAGGAGAGA 
CTGTGTAATG AGAAGAATCC ATGTCCTGTC CCGATGTCCT GGGTCTCCTG GAGCGCCTGG 
TCCAAATGTA GCGTGGCCTG CGGTGGTGGA GTCCAATCCC GCGTTCGAAC CTGCGAGAAT 
GGCAACACGT GTCCTGGATG CCCTCTGGAG TACAGAGCGT GTAATTTGGA CTCGTGTGCT 
GAAGTGAGGC GAACGACACC GTGGACCCCC TGGTATCCTG TTAATGTGAC CCAGCTGGGA 
GCGCGGCAGG AGCAGAGGGT CCGATATACC TGTAGAGCTC TGCTGGCAGA CCCTCATGAC 
TTACAGCTCG GCAAACGCAA GATAGAGACA CGCCTCTGTC CCACCACTGA CGGAGCTGCT 
GCCTGCGAGA CTGATGGTCT GGTTGAGGAT TTGTTGAGAA TGGGTCGACC TGTAACACGA 
GTGCAAGGAG CCGCCTGGGC ATTATGGGAA ACCTGGTCTA CATGTTCAAA AGAATGTTCC 
AAAGGCTTCC GCACACGCAA ACGCTCCTGT GCCGCACCGG ATGGCAAGAG CACACCGTTC 
GCCTGCAGCG GAGCCCCGGT CGAGTATCAG GACTGCAACA CTCAACCCTG CCCAGTGAAG 
GGGGCGTGGT CATGCTGGTC TTCCTGGTCT CAGTGTTCTA CTTCCTGTGG TGGCGGTCAT 
CACCAGCGCT CCCGTTCCTG CTCGAACCCT GCGCCGGCTT ATTCTGGAGA CATCTGCATC 
GGCCTGCACA CAGAGGAGGC GCTCTGTAAC ACACACGAGT GTGAAGGTGG GTGGGGGGCG 
TGGTCAGAGT GGGCAGAGTG TAATGGGGAG GGGCTTCAGC TCCGGGCTCG TACATGTGAA 
ATAAGCCCCG CCTCCTGTCT TGGAAACTCC ACAGATCAGA GACAGTGTCG GCCCAATGAG 
AGCGCAGTGC TGTCAAAAGG ACAGAAGAGG AGCACAAACT GTGGAGGGTT TTCTCTGTTC 
CACCTGGTTC TGACTGGTGC ATGTTGTTTT CTGGGAGCCG TTTTACTCTC CATCCTGGTC 
TATGTGTACT GTCAGAGACT CCGCAAACCT CCGCAAGAAT CTGCAGTCAT CCATCCCAGC 
ACCCCAAACC ATCTCCACTA TAAAGACAAC AGCTGCGCGC CCAAGAACGA TTTATACACA 
CCCATGGAGT TCAAGACTCT CAATAAGAAC AACCTGCTTC CACCAGATGA AAGCTGCAAC 
TTCTTCCCAT CGCCCTTACC ACAGACCAAC GTCTACACCA CCACATATTA CCCGTCTCCG 
CTGGGCAAAT ACGACTTCCC TTCAATGGAT TCCCCCTGCA GCTTCATGCA CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001340298.5
CDS	229..3525
Translation	MAAMVFWAGSLSFVGLVIFHLLFLPVVTSQTPSSPPDTPDPQPEECRRKMHPVTSYTALSPWLFSFSVPGVNDYSPLTLDLSRSQLIVGARNHLFRLSLSNISLLQATEWGVDETTRRSCQSKGKTEDECQNYVRVLLLNGSRLFTCGTNAFMPVCVTRPVTDISSVLDSISGVARCPYDPRHNSTAMITESGEVYAATVTDFSSRDPIIYRSLGNMPPLRTAQYNSKWLNDPHFISAYEVSRFTYVFLRENAVEQDCGKTVFSRVARVCQNDIGGRFLLEDTWTTFMKARLNCSRSGDVPFYYHELQSTFYLPEQDLIYGVFTTNVNSIAASAVCAFNLSAITQAFNGPFRYQENPRTSWLSTPNPVPNFQCGTVNDSGPGANLTERSLQDAQRLFLMSDVVQPISTDPLVMQDNIRFSKLAVDIVQGRDTLYHVMYIGTEYGTIIKALSTNNKSLRGCYLEEMNILPGNKQEPILNLQILHSDRSLFVGLPSRVLKIPLERCSNYITEQDCLGARDPYCGWDRKEKRCTTIEDSSNMSQWSQDITKCPERNLTQDGGFSDWSTWQACNHDDGGEGISTCQCRTRACDHPRPQCGGLKCVGANIEVANCSRNGGWTPWSSWAECSTSCGIGFEVRQRSCNNPSPRHGGRVCVGQAREERLCNEKNPCPVPMSWVSWSAWSKCSVACGGGVQSRVRTCENGNTCPGCPLEYRACNLDSCAEVRRTTPWTPWYPVNVTQLGARQEQRVRYTCRALLADPHDLQLGKRKIETRLCPTTDGAAACETDGLVEDLLRMGRPVTRVQGAAWALWETWSTCSKECSKGFRTRKRSCAAPDGKSTPFACSGAPVEYQDCNTQPCPVKGAWSCWSSWSQCSTSCGGGHHQRSRSCSNPAPAYSGDICIGLHTEEALCNTHECEGGWGAWSEWAECNGEGLQLRARTCEISPASCLGNSTDQRQCRPNESAVLSKGQKRSTNCGGFSLFHLVLTGACCFLGAVLLSILVYVYCQRLRKPPQESAVIHPSTPNHLHYKDNSCAPKNDLYTPMEFKTLNKNNLLPPDESCNFFPSPLPQTNVYTTTYYPSPLGKYDFPSMDSPCSFMHS

Target ORF information:

RefSeq Version	XM_001340262.8
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5Bb (sema5bb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001340262.8

ORF Insert Sequence:

ATGGCAGCGA TGGTGTTCTG GGCCGGATCA CTGTCTTTCG TCGGACTGGT CATCTTTCAC 
CTCCTCTTTC TGCCTGTGGT CACGTCACAG ACACCATCAT CACCCCCAGA CACCCCTGAC 
CCGCAGCCTG AAGAGTGCCG GAGAAAAATG CACCCTGTTA CCTCCTACAC AGCGTTGAGT 
CCGTGGCTCT TCTCGTTCTC TGTGCCTGGA GTGAACGACT ACTCACCACT CACACTCGAC 
CTCAGCAGGA GCCAGCTGAT TGTGGGAGCC AGGAATCATC TCTTCCGGCT GAGTTTGAGT 
AACATCTCTC TTCTGCAGGC CACAGAATGG GGTGTTGACG AAACTACCAG GAGATCCTGC 
CAGAGCAAAG GAAAGACCGA GGACGAGTGC CAGAATTATG TGCGAGTCCT CCTGCTGAAC 
GGCAGTCGTT TGTTCACCTG CGGTACGAAC GCCTTCATGC CCGTCTGTGT GACGCGTCCC 
GTGACGGACA TCAGCAGCGT TCTGGACTCC ATCAGCGGTG TGGCTCGCTG TCCCTACGAT 
CCTCGACACA ACTCCACAGC CATGATCACA GAGAGCGGAG AGGTGTACGC CGCCACTGTG 
ACCGATTTCT CTAGCCGGGA CCCCATCATT TACCGCAGCC TGGGGAACAT GCCGCCTCTG 
CGCACCGCAC AGTACAACTC TAAATGGCTC AACGATCCTC ACTTCATCTC GGCGTATGAG 
GTGAGCCGTT TCACATACGT GTTTCTGCGA GAGAACGCAG TGGAGCAGGA TTGTGGGAAG 
ACGGTTTTCT CTCGCGTGGC TCGCGTGTGT CAGAACGATA TCGGCGGTCG CTTCCTTCTG 
GAAGACACGT GGACCACATT CATGAAGGCC CGGCTAAACT GCTCACGATC TGGAGACGTT 
CCCTTCTACT ACCATGAGCT GCAGAGCACT TTCTATTTGC CCGAACAAGA CCTCATCTAC 
GGGGTCTTCA CCACCAATGT GAACAGTATT GCTGCGTCAG CCGTGTGTGC GTTTAACCTC 
AGTGCCATCA CTCAGGCCTT TAACGGGCCT TTCCGCTATC AGGAGAACCC TCGCACCAGC 
TGGCTGTCCA CTCCAAACCC CGTCCCAAAC TTCCAGTGCG GGACGGTGAA TGATTCGGGG 
CCGGGTGCTA ATCTGACAGA GCGCAGTCTT CAAGACGCCC AGCGGCTCTT TCTGATGAGC 
GACGTGGTTC AGCCCATCTC CACAGACCCG CTGGTCATGC AGGACAACAT CCGCTTCTCC 
AAACTGGCGG TGGACATCGT TCAGGGCAGA GACACGCTTT ACCACGTCAT GTACATCGGC 
ACAGAATATG GCACCATCAT CAAGGCCTTG TCCACTAATA ATAAGAGTCT GCGTGGATGT 
TATTTGGAGG AGATGAACAT TCTGCCGGGA AACAAGCAGG AGCCCATCCT GAACCTGCAG 
ATTCTGCACA GCGATAGATC TCTGTTTGTG GGGCTTCCCA GCAGAGTGTT GAAGATCCCT 
TTGGAAAGAT GCTCTAACTA CATCACTGAA CAGGATTGTC TGGGTGCCAG GGATCCGTAC 
TGCGGCTGGG ACCGTAAAGA GAAGCGCTGC ACCACCATTG AGGACAGCTC CAACATGAGC 
CAATGGAGCC AGGACATCAC AAAGTGCCCT GAGAGGAACC TCACTCAAGA TGGCGGCTTT 
AGTGATTGGT CAACATGGCA GGCTTGTAAC CATGATGACG GAGGGGAAGG CATCAGCACA 
TGCCAGTGTC GCACCCGGGC CTGCGATCAC CCTCGACCCC AGTGTGGAGG ACTAAAGTGT 
GTCGGGGCTA ATATAGAGGT GGCCAACTGC TCCAGGAATG GAGGCTGGAC ACCCTGGTCA 
TCCTGGGCCG AGTGCAGCAC CAGCTGTGGG ATTGGGTTTG AGGTCCGTCA GCGATCCTGT 
AACAATCCTT CACCTCGACA CGGAGGACGC GTGTGTGTAG GCCAGGCCCG AGAGGAGAGA 
CTGTGTAATG AGAAGAATCC ATGTCCTGTC CCGATGTCCT GGGTCTCCTG GAGCGCCTGG 
TCCAAATGTA GCGTGGCCTG CGGTGGTGGA GTCCAATCCC GCGTTCGAAC CTGCGAGAAT 
GGCAACACGT GTCCTGGATG CCCTCTGGAG TACAGAGCGT GTAATTTGGA CTCGTGTGCT 
GAAGTGAGGC GAACGACACC GTGGACCCCC TGGTATCCTG TTAATGTGAC CCAGCTGGGA 
GCGCGGCAGG AGCAGAGGGT CCGATATACC TGTAGAGCTC TGCTGGCAGA CCCTCATGAC 
TTACAGCTCG GCAAACGCAA GATAGAGACA CGCCTCTGTC CCACCACTGA CGGAGCTGCT 
GCCTGCGAGA CTGATGGTCT GGTTGAGGAT TTGTTGAGAA TGGGTCGACC TGTAACACGA 
GTGCAAGGAG CCGCCTGGGC ATTATGGGAA ACCTGGTCTA CATGTTCAAA AGAATGTTCC 
AAAGGCTTCC GCACACGCAA ACGCTCCTGT GCCGCACCGG ATGGCAAGAG CACACCGTTC 
GCCTGCAGCG GAGCCCCGGT CGAGTATCAG GACTGCAACA CTCAACCCTG CCCAGTGAAG 
GGGGCGTGGT CATGCTGGTC TTCCTGGTCT CAGTGTTCTA CTTCCTGTGG TGGCGGTCAT 
CACCAGCGCT CCCGTTCCTG CTCGAACCCT GCGCCGGCTT ATTCTGGAGA CATCTGCATC 
GGCCTGCACA CAGAGGAGGC GCTCTGTAAC ACACACGAGT GTGAAGGTGG GTGGGGGGCG 
TGGTCAGAGT GGGCAGAGTG TAATGGGGAG GGGCTTCAGC TCCGGGCTCG TACATGTGAA 
ATAAGCCCCG CCTCCTGTCT TGGAAACTCC ACAGATCAGA GACAGTGTCG GCCCAATGAG 
AGCGCAGTGC TGTCAAAAGG ACAGAAGAGG AGCACAAACT GTGGAGGGTT TTCTCTGTTC 
CACCTGGTTC TGACTGGTGC ATGTTGTTTT CTGGGAGCCG TTTTACTCTC CATCCTGGTC 
TATGTGTACT GTCAGAGACT CCGCAAACCT CCGCAAGAAT CTGCAGTCAT CCATCCCAGC 
ACCCCAAACC ATCTCCACTA TAAAGACAAC AGCTGCGCGC CCAAGAACGA TTTATACACA 
CCCATGGAGT TCAAGACTCT CAATAAGAAC AACCTGCTTC CACCAGATGA AAGCTGCAAC 
TTCTTCCCAT CGCCCTTACC ACAGACCAAC GTCTACACCA CCACATATTA CCCGTCTCCG 
CTGGGCAAAT ACGACTTCCC TTCAATGGAT TCCCCCTGCA GCTTCATGCA CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA5BB ( XM_017356553.2 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_017212042.1 Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5Bb (sema5bb), transcript variant X2, mRNA.

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CloneID	ODa11406
Clone ID Related Accession (Same CDS sequence)	XM_017356553.1 , XM_001340262.8 , XM_017356553.2 , XM_001340262.7
Accession Version	XM_017356553.2 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2017-06-14
Organism	Danio rerio(zebrafish)
Product	semaphorin-5B
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.7) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 15, 2017 this sequence version replaced XM_017356553.1. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 106 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.4 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGA TGGTGTTCTG GGCCGGATCA CTGTCTTTCG TCGGACTGGT CATCTTTCAC 
CTCCTCTTTC TGCCTGTGGT CACGTCACAG ACACCATCAT CACCCCCAGA CACCCCTGAC 
CCGCAGCCTG AAGAGTGCCG GAGAAAAATG CACCCTGTTA CCTCCTACAC AGCGTTGAGT 
CCGTGGCTCT TCTCGTTCTC TGTGCCTGGA GTGAACGACT ACTCACCACT CACACTCGAC 
CTCAGCAGGA GCCAGCTGAT TGTGGGAGCC AGGAATCATC TCTTCCGGCT GAGTTTGAGT 
AACATCTCTC TTCTGCAGGC CACAGAATGG GGTGTTGACG AAACTACCAG GAGATCCTGC 
CAGAGCAAAG GAAAGACCGA GGACGAGTGC CAGAATTATG TGCGAGTCCT CCTGCTGAAC 
GGCAGTCGTT TGTTCACCTG CGGTACGAAC GCCTTCATGC CCGTCTGTGT GACGCGTCCC 
GTGACGGACA TCAGCAGCGT TCTGGACTCC ATCAGCGGTG TGGCTCGCTG TCCCTACGAT 
CCTCGACACA ACTCCACAGC CATGATCACA GAGAGCGGAG AGGTGTACGC CGCCACTGTG 
ACCGATTTCT CTAGCCGGGA CCCCATCATT TACCGCAGCC TGGGGAACAT GCCGCCTCTG 
CGCACCGCAC AGTACAACTC TAAATGGCTC AACGATCCTC ACTTCATCTC GGCGTATGAG 
GTGAGCCGTT TCACATACGT GTTTCTGCGA GAGAACGCAG TGGAGCAGGA TTGTGGGAAG 
ACGGTTTTCT CTCGCGTGGC TCGCGTGTGT CAGAACGATA TCGGCGGTCG CTTCCTTCTG 
GAAGACACGT GGACCACATT CATGAAGGCC CGGCTAAACT GCTCACGATC TGGAGACGTT 
CCCTTCTACT ACCATGAGCT GCAGAGCACT TTCTATTTGC CCGAACAAGA CCTCATCTAC 
GGGGTCTTCA CCACCAATGT GAACAGTATT GCTGCGTCAG CCGTGTGTGC GTTTAACCTC 
AGTGCCATCA CTCAGGCCTT TAACGGGCCT TTCCGCTATC AGGAGAACCC TCGCACCAGC 
TGGCTGTCCA CTCCAAACCC CGTCCCAAAC TTCCAGTGCG GGACGGTGAA TGATTCGGGG 
CCGGGTGCTA ATCTGACAGA GCGCAGTCTT CAAGACGCCC AGCGGCTCTT TCTGATGAGC 
GACGTGGTTC AGCCCATCTC CACAGACCCG CTGGTCATGC AGGACAACAT CCGCTTCTCC 
AAACTGGCGG TGGACATCGT TCAGGGCAGA GACACGCTTT ACCACGTCAT GTACATCGGC 
ACAGAATATG GCACCATCAT CAAGGCCTTG TCCACTAATA ATAAGAGTCT GCGTGGATGT 
TATTTGGAGG AGATGAACAT TCTGCCGGGA AACAAGCAGG AGCCCATCCT GAACCTGCAG 
ATTCTGCACA GCGATAGATC TCTGTTTGTG GGGCTTCCCA GCAGAGTGTT GAAGATCCCT 
TTGGAAAGAT GCTCTAACTA CATCACTGAA CAGGATTGTC TGGGTGCCAG GGATCCGTAC 
TGCGGCTGGG ACCGTAAAGA GAAGCGCTGC ACCACCATTG AGGACAGCTC CAACATGAGC 
CAATGGAGCC AGGACATCAC AAAGTGCCCT GAGAGGAACC TCACTCAAGA TGGCGGCTTT 
AGTGATTGGT CAACATGGCA GGCTTGTAAC CATGATGACG GAGGGGAAGG CATCAGCACA 
TGCCAGTGTC GCACCCGGGC CTGCGATCAC CCTCGACCCC AGTGTGGAGG ACTAAAGTGT 
GTCGGGGCTA ATATAGAGGT GGCCAACTGC TCCAGGAATG GAGGCTGGAC ACCCTGGTCA 
TCCTGGGCCG AGTGCAGCAC CAGCTGTGGG ATTGGGTTTG AGGTCCGTCA GCGATCCTGT 
AACAATCCTT CACCTCGACA CGGAGGACGC GTGTGTGTAG GCCAGGCCCG AGAGGAGAGA 
CTGTGTAATG AGAAGAATCC ATGTCCTGTC CCGATGTCCT GGGTCTCCTG GAGCGCCTGG 
TCCAAATGTA GCGTGGCCTG CGGTGGTGGA GTCCAATCCC GCGTTCGAAC CTGCGAGAAT 
GGCAACACGT GTCCTGGATG CCCTCTGGAG TACAGAGCGT GTAATTTGGA CTCGTGTGCT 
GAAGTGAGGC GAACGACACC GTGGACCCCC TGGTATCCTG TTAATGTGAC CCAGCTGGGA 
GCGCGGCAGG AGCAGAGGGT CCGATATACC TGTAGAGCTC TGCTGGCAGA CCCTCATGAC 
TTACAGCTCG GCAAACGCAA GATAGAGACA CGCCTCTGTC CCACCACTGA CGGAGCTGCT 
GCCTGCGAGA CTGATGGTCT GGTTGAGGAT TTGTTGAGAA TGGGTCGACC TGTAACACGA 
GTGCAAGGAG CCGCCTGGGC ATTATGGGAA ACCTGGTCTA CATGTTCAAA AGAATGTTCC 
AAAGGCTTCC GCACACGCAA ACGCTCCTGT GCCGCACCGG ATGGCAAGAG CACACCGTTC 
GCCTGCAGCG GAGCCCCGGT CGAGTATCAG GACTGCAACA CTCAACCCTG CCCAGTGAAG 
GGGGCGTGGT CATGCTGGTC TTCCTGGTCT CAGTGTTCTA CTTCCTGTGG TGGCGGTCAT 
CACCAGCGCT CCCGTTCCTG CTCGAACCCT GCGCCGGCTT ATTCTGGAGA CATCTGCATC 
GGCCTGCACA CAGAGGAGGC GCTCTGTAAC ACACACGAGT GTGAAGGTGG GTGGGGGGCG 
TGGTCAGAGT GGGCAGAGTG TAATGGGGAG GGGCTTCAGC TCCGGGCTCG TACATGTGAA 
ATAAGCCCCG CCTCCTGTCT TGGAAACTCC ACAGATCAGA GACAGTGTCG GCCCAATGAG 
AGCGCAGTGC TGTCAAAAGG ACAGAAGAGG AGCACAAACT GTGGAGGGTT TTCTCTGTTC 
CACCTGGTTC TGACTGGTGC ATGTTGTTTT CTGGGAGCCG TTTTACTCTC CATCCTGGTC 
TATGTGTACT GTCAGAGACT CCGCAAACCT CCGCAAGAAT CTGCAGTCAT CCATCCCAGC 
ACCCCAAACC ATCTCCACTA TAAAGACAAC AGCTGCGCGC CCAAGAACGA TTTATACACA 
CCCATGGAGT TCAAGACTCT CAATAAGAAC AACCTGCTTC CACCAGATGA AAGCTGCAAC 
TTCTTCCCAT CGCCCTTACC ACAGACCAAC GTCTACACCA CCACATATTA CCCGTCTCCG 
CTGGGCAAAT ACGACTTCCC TTCAATGGAT TCCCCCTGCA GCTTCATGCA CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_017212042.1
CDS	550..3846
Translation	MAAMVFWAGSLSFVGLVIFHLLFLPVVTSQTPSSPPDTPDPQPEECRRKMHPVTSYTALSPWLFSFSVPGVNDYSPLTLDLSRSQLIVGARNHLFRLSLSNISLLQATEWGVDETTRRSCQSKGKTEDECQNYVRVLLLNGSRLFTCGTNAFMPVCVTRPVTDISSVLDSISGVARCPYDPRHNSTAMITESGEVYAATVTDFSSRDPIIYRSLGNMPPLRTAQYNSKWLNDPHFISAYEVSRFTYVFLRENAVEQDCGKTVFSRVARVCQNDIGGRFLLEDTWTTFMKARLNCSRSGDVPFYYHELQSTFYLPEQDLIYGVFTTNVNSIAASAVCAFNLSAITQAFNGPFRYQENPRTSWLSTPNPVPNFQCGTVNDSGPGANLTERSLQDAQRLFLMSDVVQPISTDPLVMQDNIRFSKLAVDIVQGRDTLYHVMYIGTEYGTIIKALSTNNKSLRGCYLEEMNILPGNKQEPILNLQILHSDRSLFVGLPSRVLKIPLERCSNYITEQDCLGARDPYCGWDRKEKRCTTIEDSSNMSQWSQDITKCPERNLTQDGGFSDWSTWQACNHDDGGEGISTCQCRTRACDHPRPQCGGLKCVGANIEVANCSRNGGWTPWSSWAECSTSCGIGFEVRQRSCNNPSPRHGGRVCVGQAREERLCNEKNPCPVPMSWVSWSAWSKCSVACGGGVQSRVRTCENGNTCPGCPLEYRACNLDSCAEVRRTTPWTPWYPVNVTQLGARQEQRVRYTCRALLADPHDLQLGKRKIETRLCPTTDGAAACETDGLVEDLLRMGRPVTRVQGAAWALWETWSTCSKECSKGFRTRKRSCAAPDGKSTPFACSGAPVEYQDCNTQPCPVKGAWSCWSSWSQCSTSCGGGHHQRSRSCSNPAPAYSGDICIGLHTEEALCNTHECEGGWGAWSEWAECNGEGLQLRARTCEISPASCLGNSTDQRQCRPNESAVLSKGQKRSTNCGGFSLFHLVLTGACCFLGAVLLSILVYVYCQRLRKPPQESAVIHPSTPNHLHYKDNSCAPKNDLYTPMEFKTLNKNNLLPPDESCNFFPSPLPQTNVYTTTYYPSPLGKYDFPSMDSPCSFMHS

Target ORF information:

RefSeq Version	XM_017356553.2
Organism	Danio rerio(zebrafish)
Definition	Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5Bb (sema5bb), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_017356553.2

ORF Insert Sequence:

ATGGCAGCGA TGGTGTTCTG GGCCGGATCA CTGTCTTTCG TCGGACTGGT CATCTTTCAC 
CTCCTCTTTC TGCCTGTGGT CACGTCACAG ACACCATCAT CACCCCCAGA CACCCCTGAC 
CCGCAGCCTG AAGAGTGCCG GAGAAAAATG CACCCTGTTA CCTCCTACAC AGCGTTGAGT 
CCGTGGCTCT TCTCGTTCTC TGTGCCTGGA GTGAACGACT ACTCACCACT CACACTCGAC 
CTCAGCAGGA GCCAGCTGAT TGTGGGAGCC AGGAATCATC TCTTCCGGCT GAGTTTGAGT 
AACATCTCTC TTCTGCAGGC CACAGAATGG GGTGTTGACG AAACTACCAG GAGATCCTGC 
CAGAGCAAAG GAAAGACCGA GGACGAGTGC CAGAATTATG TGCGAGTCCT CCTGCTGAAC 
GGCAGTCGTT TGTTCACCTG CGGTACGAAC GCCTTCATGC CCGTCTGTGT GACGCGTCCC 
GTGACGGACA TCAGCAGCGT TCTGGACTCC ATCAGCGGTG TGGCTCGCTG TCCCTACGAT 
CCTCGACACA ACTCCACAGC CATGATCACA GAGAGCGGAG AGGTGTACGC CGCCACTGTG 
ACCGATTTCT CTAGCCGGGA CCCCATCATT TACCGCAGCC TGGGGAACAT GCCGCCTCTG 
CGCACCGCAC AGTACAACTC TAAATGGCTC AACGATCCTC ACTTCATCTC GGCGTATGAG 
GTGAGCCGTT TCACATACGT GTTTCTGCGA GAGAACGCAG TGGAGCAGGA TTGTGGGAAG 
ACGGTTTTCT CTCGCGTGGC TCGCGTGTGT CAGAACGATA TCGGCGGTCG CTTCCTTCTG 
GAAGACACGT GGACCACATT CATGAAGGCC CGGCTAAACT GCTCACGATC TGGAGACGTT 
CCCTTCTACT ACCATGAGCT GCAGAGCACT TTCTATTTGC CCGAACAAGA CCTCATCTAC 
GGGGTCTTCA CCACCAATGT GAACAGTATT GCTGCGTCAG CCGTGTGTGC GTTTAACCTC 
AGTGCCATCA CTCAGGCCTT TAACGGGCCT TTCCGCTATC AGGAGAACCC TCGCACCAGC 
TGGCTGTCCA CTCCAAACCC CGTCCCAAAC TTCCAGTGCG GGACGGTGAA TGATTCGGGG 
CCGGGTGCTA ATCTGACAGA GCGCAGTCTT CAAGACGCCC AGCGGCTCTT TCTGATGAGC 
GACGTGGTTC AGCCCATCTC CACAGACCCG CTGGTCATGC AGGACAACAT CCGCTTCTCC 
AAACTGGCGG TGGACATCGT TCAGGGCAGA GACACGCTTT ACCACGTCAT GTACATCGGC 
ACAGAATATG GCACCATCAT CAAGGCCTTG TCCACTAATA ATAAGAGTCT GCGTGGATGT 
TATTTGGAGG AGATGAACAT TCTGCCGGGA AACAAGCAGG AGCCCATCCT GAACCTGCAG 
ATTCTGCACA GCGATAGATC TCTGTTTGTG GGGCTTCCCA GCAGAGTGTT GAAGATCCCT 
TTGGAAAGAT GCTCTAACTA CATCACTGAA CAGGATTGTC TGGGTGCCAG GGATCCGTAC 
TGCGGCTGGG ACCGTAAAGA GAAGCGCTGC ACCACCATTG AGGACAGCTC CAACATGAGC 
CAATGGAGCC AGGACATCAC AAAGTGCCCT GAGAGGAACC TCACTCAAGA TGGCGGCTTT 
AGTGATTGGT CAACATGGCA GGCTTGTAAC CATGATGACG GAGGGGAAGG CATCAGCACA 
TGCCAGTGTC GCACCCGGGC CTGCGATCAC CCTCGACCCC AGTGTGGAGG ACTAAAGTGT 
GTCGGGGCTA ATATAGAGGT GGCCAACTGC TCCAGGAATG GAGGCTGGAC ACCCTGGTCA 
TCCTGGGCCG AGTGCAGCAC CAGCTGTGGG ATTGGGTTTG AGGTCCGTCA GCGATCCTGT 
AACAATCCTT CACCTCGACA CGGAGGACGC GTGTGTGTAG GCCAGGCCCG AGAGGAGAGA 
CTGTGTAATG AGAAGAATCC ATGTCCTGTC CCGATGTCCT GGGTCTCCTG GAGCGCCTGG 
TCCAAATGTA GCGTGGCCTG CGGTGGTGGA GTCCAATCCC GCGTTCGAAC CTGCGAGAAT 
GGCAACACGT GTCCTGGATG CCCTCTGGAG TACAGAGCGT GTAATTTGGA CTCGTGTGCT 
GAAGTGAGGC GAACGACACC GTGGACCCCC TGGTATCCTG TTAATGTGAC CCAGCTGGGA 
GCGCGGCAGG AGCAGAGGGT CCGATATACC TGTAGAGCTC TGCTGGCAGA CCCTCATGAC 
TTACAGCTCG GCAAACGCAA GATAGAGACA CGCCTCTGTC CCACCACTGA CGGAGCTGCT 
GCCTGCGAGA CTGATGGTCT GGTTGAGGAT TTGTTGAGAA TGGGTCGACC TGTAACACGA 
GTGCAAGGAG CCGCCTGGGC ATTATGGGAA ACCTGGTCTA CATGTTCAAA AGAATGTTCC 
AAAGGCTTCC GCACACGCAA ACGCTCCTGT GCCGCACCGG ATGGCAAGAG CACACCGTTC 
GCCTGCAGCG GAGCCCCGGT CGAGTATCAG GACTGCAACA CTCAACCCTG CCCAGTGAAG 
GGGGCGTGGT CATGCTGGTC TTCCTGGTCT CAGTGTTCTA CTTCCTGTGG TGGCGGTCAT 
CACCAGCGCT CCCGTTCCTG CTCGAACCCT GCGCCGGCTT ATTCTGGAGA CATCTGCATC 
GGCCTGCACA CAGAGGAGGC GCTCTGTAAC ACACACGAGT GTGAAGGTGG GTGGGGGGCG 
TGGTCAGAGT GGGCAGAGTG TAATGGGGAG GGGCTTCAGC TCCGGGCTCG TACATGTGAA 
ATAAGCCCCG CCTCCTGTCT TGGAAACTCC ACAGATCAGA GACAGTGTCG GCCCAATGAG 
AGCGCAGTGC TGTCAAAAGG ACAGAAGAGG AGCACAAACT GTGGAGGGTT TTCTCTGTTC 
CACCTGGTTC TGACTGGTGC ATGTTGTTTT CTGGGAGCCG TTTTACTCTC CATCCTGGTC 
TATGTGTACT GTCAGAGACT CCGCAAACCT CCGCAAGAAT CTGCAGTCAT CCATCCCAGC 
ACCCCAAACC ATCTCCACTA TAAAGACAAC AGCTGCGCGC CCAAGAACGA TTTATACACA 
CCCATGGAGT TCAAGACTCT CAATAAGAAC AACCTGCTTC CACCAGATGA AAGCTGCAAC 
TTCTTCCCAT CGCCCTTACC ACAGACCAAC GTCTACACCA CCACATATTA CCCGTCTCCG 
CTGGGCAAAT ACGACTTCCC TTCAATGGAT TCCCCCTGCA GCTTCATGCA CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SEMA5BB ( XM_001340262.7 ) cDNA ORF clone, Danio rerio(Zebrafish) -> XP_001340298.5 Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5Bb (sema5bb), transcript variant X1, mRNA.

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CloneID	ODa11406
Clone ID Related Accession (Same CDS sequence)	XM_017356553.1 , XM_001340262.8 , XM_017356553.2 , XM_001340262.7
Accession Version	XM_001340262.7	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3297bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-06-23
Organism	Danio rerio(Zebrafish)
Product	semaphorin-5B isoform X1
Comment	MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_007117.6) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process On Jun 24, 2016 this sequence version replaced XM_001340262.6. ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Danio rerio Annotation Release 105 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 7.1 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAGCGA TGGTGTTCTG GGCCGGATCA CTGTCTTTCG TCGGACTGGT CATCTTTCAC 
CTCCTCTTTC TGCCTGTGGT CACGTCACAG ACACCATCAT CACCCCCAGA CACCCCTGAC 
CCGCAGCCTG AAGAGTGCCG GAGAAAAATG CACCCTGTTA CCTCCTACAC AGCGTTGAGT 
CCGTGGCTCT TCTCGTTCTC TGTGCCTGGA GTGAACGACT ACTCACCACT CACACTCGAC 
CTCAGCAGGA GCCAGCTGAT TGTGGGAGCC AGGAATCATC TCTTCCGGCT GAGTTTGAGT 
AACATCTCTC TTCTGCAGGC CACAGAATGG GGTGTTGACG AAACTACCAG GAGATCCTGC 
CAGAGCAAAG GAAAGACCGA GGACGAGTGC CAGAATTATG TGCGAGTCCT CCTGCTGAAC 
GGCAGTCGTT TGTTCACCTG CGGTACGAAC GCCTTCATGC CCGTCTGTGT GACGCGTCCC 
GTGACGGACA TCAGCAGCGT TCTGGACTCC ATCAGCGGTG TGGCTCGCTG TCCCTACGAT 
CCTCGACACA ACTCCACAGC CATGATCACA GAGAGCGGAG AGGTGTACGC CGCCACTGTG 
ACCGATTTCT CTAGCCGGGA CCCCATCATT TACCGCAGCC TGGGGAACAT GCCGCCTCTG 
CGCACCGCAC AGTACAACTC TAAATGGCTC AACGATCCTC ACTTCATCTC GGCGTATGAG 
GTGAGCCGTT TCACATACGT GTTTCTGCGA GAGAACGCAG TGGAGCAGGA TTGTGGGAAG 
ACGGTTTTCT CTCGCGTGGC TCGCGTGTGT CAGAACGATA TCGGCGGTCG CTTCCTTCTG 
GAAGACACGT GGACCACATT CATGAAGGCC CGGCTAAACT GCTCACGATC TGGAGACGTT 
CCCTTCTACT ACCATGAGCT GCAGAGCACT TTCTATTTGC CCGAACAAGA CCTCATCTAC 
GGGGTCTTCA CCACCAATGT GAACAGTATT GCTGCGTCAG CCGTGTGTGC GTTTAACCTC 
AGTGCCATCA CTCAGGCCTT TAACGGGCCT TTCCGCTATC AGGAGAACCC TCGCACCAGC 
TGGCTGTCCA CTCCAAACCC CGTCCCAAAC TTCCAGTGCG GGACGGTGAA TGATTCGGGG 
CCGGGTGCTA ATCTGACAGA GCGCAGTCTT CAAGACGCCC AGCGGCTCTT TCTGATGAGC 
GACGTGGTTC AGCCCATCTC CACAGACCCG CTGGTCATGC AGGACAACAT CCGCTTCTCC 
AAACTGGCGG TGGACATCGT TCAGGGCAGA GACACGCTTT ACCACGTCAT GTACATCGGC 
ACAGAATATG GCACCATCAT CAAGGCCTTG TCCACTAATA ATAAGAGTCT GCGTGGATGT 
TATTTGGAGG AGATGAACAT TCTGCCGGGA AACAAGCAGG AGCCCATCCT GAACCTGCAG 
ATTCTGCACA GCGATAGATC TCTGTTTGTG GGGCTTCCCA GCAGAGTGTT GAAGATCCCT 
TTGGAAAGAT GCTCTAACTA CATCACTGAA CAGGATTGTC TGGGTGCCAG GGATCCGTAC 
TGCGGCTGGG ACCGTAAAGA GAAGCGCTGC ACCACCATTG AGGACAGCTC CAACATGAGC 
CAATGGAGCC AGGACATCAC AAAGTGCCCT GAGAGGAACC TCACTCAAGA TGGCGGCTTT 
AGTGATTGGT CAACATGGCA GGCTTGTAAC CATGATGACG GAGGGGAAGG CATCAGCACA 
TGCCAGTGTC GCACCCGGGC CTGCGATCAC CCTCGACCCC AGTGTGGAGG ACTAAAGTGT 
GTCGGGGCTA ATATAGAGGT GGCCAACTGC TCCAGGAATG GAGGCTGGAC ACCCTGGTCA 
TCCTGGGCCG AGTGCAGCAC CAGCTGTGGG ATTGGGTTTG AGGTCCGTCA GCGATCCTGT 
AACAATCCTT CACCTCGACA CGGAGGACGC GTGTGTGTAG GCCAGGCCCG AGAGGAGAGA 
CTGTGTAATG AGAAGAATCC ATGTCCTGTC CCGATGTCCT GGGTCTCCTG GAGCGCCTGG 
TCCAAATGTA GCGTGGCCTG CGGTGGTGGA GTCCAATCCC GCGTTCGAAC CTGCGAGAAT 
GGCAACACGT GTCCTGGATG CCCTCTGGAG TACAGAGCGT GTAATTTGGA CTCGTGTGCT 
GAAGTGAGGC GAACGACACC GTGGACCCCC TGGTATCCTG TTAATGTGAC CCAGCTGGGA 
GCGCGGCAGG AGCAGAGGGT CCGATATACC TGTAGAGCTC TGCTGGCAGA CCCTCATGAC 
TTACAGCTCG GCAAACGCAA GATAGAGACA CGCCTCTGTC CCACCACTGA CGGAGCTGCT 
GCCTGCGAGA CTGATGGTCT GGTTGAGGAT TTGTTGAGAA TGGGTCGACC TGTAACACGA 
GTGCAAGGAG CCGCCTGGGC ATTATGGGAA ACCTGGTCTA CATGTTCAAA AGAATGTTCC 
AAAGGCTTCC GCACACGCAA ACGCTCCTGT GCCGCACCGG ATGGCAAGAG CACACCGTTC 
GCCTGCAGCG GAGCCCCGGT CGAGTATCAG GACTGCAACA CTCAACCCTG CCCAGTGAAG 
GGGGCGTGGT CATGCTGGTC TTCCTGGTCT CAGTGTTCTA CTTCCTGTGG TGGCGGTCAT 
CACCAGCGCT CCCGTTCCTG CTCGAACCCT GCGCCGGCTT ATTCTGGAGA CATCTGCATC 
GGCCTGCACA CAGAGGAGGC GCTCTGTAAC ACACACGAGT GTGAAGGTGG GTGGGGGGCG 
TGGTCAGAGT GGGCAGAGTG TAATGGGGAG GGGCTTCAGC TCCGGGCTCG TACATGTGAA 
ATAAGCCCCG CCTCCTGTCT TGGAAACTCC ACAGATCAGA GACAGTGTCG GCCCAATGAG 
AGCGCAGTGC TGTCAAAAGG ACAGAAGAGG AGCACAAACT GTGGAGGGTT TTCTCTGTTC 
CACCTGGTTC TGACTGGTGC ATGTTGTTTT CTGGGAGCCG TTTTACTCTC CATCCTGGTC 
TATGTGTACT GTCAGAGACT CCGCAAACCT CCGCAAGAAT CTGCAGTCAT CCATCCCAGC 
ACCCCAAACC ATCTCCACTA TAAAGACAAC AGCTGCGCGC CCAAGAACGA TTTATACACA 
CCCATGGAGT TCAAGACTCT CAATAAGAAC AACCTGCTTC CACCAGATGA AAGCTGCAAC 
TTCTTCCCAT CGCCCTTACC ACAGACCAAC GTCTACACCA CCACATATTA CCCGTCTCCG 
CTGGGCAAAT ACGACTTCCC TTCAATGGAT TCCCCCTGCA GCTTCATGCA CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_001340298.5
CDS	222..3518
Translation	MAAMVFWAGSLSFVGLVIFHLLFLPVVTSQTPSSPPDTPDPQPEECRRKMHPVTSYTALSPWLFSFSVPGVNDYSPLTLDLSRSQLIVGARNHLFRLSLSNISLLQATEWGVDETTRRSCQSKGKTEDECQNYVRVLLLNGSRLFTCGTNAFMPVCVTRPVTDISSVLDSISGVARCPYDPRHNSTAMITESGEVYAATVTDFSSRDPIIYRSLGNMPPLRTAQYNSKWLNDPHFISAYEVSRFTYVFLRENAVEQDCGKTVFSRVARVCQNDIGGRFLLEDTWTTFMKARLNCSRSGDVPFYYHELQSTFYLPEQDLIYGVFTTNVNSIAASAVCAFNLSAITQAFNGPFRYQENPRTSWLSTPNPVPNFQCGTVNDSGPGANLTERSLQDAQRLFLMSDVVQPISTDPLVMQDNIRFSKLAVDIVQGRDTLYHVMYIGTEYGTIIKALSTNNKSLRGCYLEEMNILPGNKQEPILNLQILHSDRSLFVGLPSRVLKIPLERCSNYITEQDCLGARDPYCGWDRKEKRCTTIEDSSNMSQWSQDITKCPERNLTQDGGFSDWSTWQACNHDDGGEGISTCQCRTRACDHPRPQCGGLKCVGANIEVANCSRNGGWTPWSSWAECSTSCGIGFEVRQRSCNNPSPRHGGRVCVGQAREERLCNEKNPCPVPMSWVSWSAWSKCSVACGGGVQSRVRTCENGNTCPGCPLEYRACNLDSCAEVRRTTPWTPWYPVNVTQLGARQEQRVRYTCRALLADPHDLQLGKRKIETRLCPTTDGAAACETDGLVEDLLRMGRPVTRVQGAAWALWETWSTCSKECSKGFRTRKRSCAAPDGKSTPFACSGAPVEYQDCNTQPCPVKGAWSCWSSWSQCSTSCGGGHHQRSRSCSNPAPAYSGDICIGLHTEEALCNTHECEGGWGAWSEWAECNGEGLQLRARTCEISPASCLGNSTDQRQCRPNESAVLSKGQKRSTNCGGFSLFHLVLTGACCFLGAVLLSILVYVYCQRLRKPPQESAVIHPSTPNHLHYKDNSCAPKNDLYTPMEFKTLNKNNLLPPDESCNFFPSPLPQTNVYTTTYYPSPLGKYDFPSMDSPCSFMHS

Target ORF information:

RefSeq Version	XM_001340262.7
Organism	Danio rerio(Zebrafish)
Definition	Danio rerio sema domain, seven thrombospondin repeats (type 1 and type 1-like), transmembrane domain (TM) and short cytoplasmic domain, (semaphorin) 5Bb (sema5bb), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_001340262.7

ORF Insert Sequence:

ATGGCAGCGA TGGTGTTCTG GGCCGGATCA CTGTCTTTCG TCGGACTGGT CATCTTTCAC 
CTCCTCTTTC TGCCTGTGGT CACGTCACAG ACACCATCAT CACCCCCAGA CACCCCTGAC 
CCGCAGCCTG AAGAGTGCCG GAGAAAAATG CACCCTGTTA CCTCCTACAC AGCGTTGAGT 
CCGTGGCTCT TCTCGTTCTC TGTGCCTGGA GTGAACGACT ACTCACCACT CACACTCGAC 
CTCAGCAGGA GCCAGCTGAT TGTGGGAGCC AGGAATCATC TCTTCCGGCT GAGTTTGAGT 
AACATCTCTC TTCTGCAGGC CACAGAATGG GGTGTTGACG AAACTACCAG GAGATCCTGC 
CAGAGCAAAG GAAAGACCGA GGACGAGTGC CAGAATTATG TGCGAGTCCT CCTGCTGAAC 
GGCAGTCGTT TGTTCACCTG CGGTACGAAC GCCTTCATGC CCGTCTGTGT GACGCGTCCC 
GTGACGGACA TCAGCAGCGT TCTGGACTCC ATCAGCGGTG TGGCTCGCTG TCCCTACGAT 
CCTCGACACA ACTCCACAGC CATGATCACA GAGAGCGGAG AGGTGTACGC CGCCACTGTG 
ACCGATTTCT CTAGCCGGGA CCCCATCATT TACCGCAGCC TGGGGAACAT GCCGCCTCTG 
CGCACCGCAC AGTACAACTC TAAATGGCTC AACGATCCTC ACTTCATCTC GGCGTATGAG 
GTGAGCCGTT TCACATACGT GTTTCTGCGA GAGAACGCAG TGGAGCAGGA TTGTGGGAAG 
ACGGTTTTCT CTCGCGTGGC TCGCGTGTGT CAGAACGATA TCGGCGGTCG CTTCCTTCTG 
GAAGACACGT GGACCACATT CATGAAGGCC CGGCTAAACT GCTCACGATC TGGAGACGTT 
CCCTTCTACT ACCATGAGCT GCAGAGCACT TTCTATTTGC CCGAACAAGA CCTCATCTAC 
GGGGTCTTCA CCACCAATGT GAACAGTATT GCTGCGTCAG CCGTGTGTGC GTTTAACCTC 
AGTGCCATCA CTCAGGCCTT TAACGGGCCT TTCCGCTATC AGGAGAACCC TCGCACCAGC 
TGGCTGTCCA CTCCAAACCC CGTCCCAAAC TTCCAGTGCG GGACGGTGAA TGATTCGGGG 
CCGGGTGCTA ATCTGACAGA GCGCAGTCTT CAAGACGCCC AGCGGCTCTT TCTGATGAGC 
GACGTGGTTC AGCCCATCTC CACAGACCCG CTGGTCATGC AGGACAACAT CCGCTTCTCC 
AAACTGGCGG TGGACATCGT TCAGGGCAGA GACACGCTTT ACCACGTCAT GTACATCGGC 
ACAGAATATG GCACCATCAT CAAGGCCTTG TCCACTAATA ATAAGAGTCT GCGTGGATGT 
TATTTGGAGG AGATGAACAT TCTGCCGGGA AACAAGCAGG AGCCCATCCT GAACCTGCAG 
ATTCTGCACA GCGATAGATC TCTGTTTGTG GGGCTTCCCA GCAGAGTGTT GAAGATCCCT 
TTGGAAAGAT GCTCTAACTA CATCACTGAA CAGGATTGTC TGGGTGCCAG GGATCCGTAC 
TGCGGCTGGG ACCGTAAAGA GAAGCGCTGC ACCACCATTG AGGACAGCTC CAACATGAGC 
CAATGGAGCC AGGACATCAC AAAGTGCCCT GAGAGGAACC TCACTCAAGA TGGCGGCTTT 
AGTGATTGGT CAACATGGCA GGCTTGTAAC CATGATGACG GAGGGGAAGG CATCAGCACA 
TGCCAGTGTC GCACCCGGGC CTGCGATCAC CCTCGACCCC AGTGTGGAGG ACTAAAGTGT 
GTCGGGGCTA ATATAGAGGT GGCCAACTGC TCCAGGAATG GAGGCTGGAC ACCCTGGTCA 
TCCTGGGCCG AGTGCAGCAC CAGCTGTGGG ATTGGGTTTG AGGTCCGTCA GCGATCCTGT 
AACAATCCTT CACCTCGACA CGGAGGACGC GTGTGTGTAG GCCAGGCCCG AGAGGAGAGA 
CTGTGTAATG AGAAGAATCC ATGTCCTGTC CCGATGTCCT GGGTCTCCTG GAGCGCCTGG 
TCCAAATGTA GCGTGGCCTG CGGTGGTGGA GTCCAATCCC GCGTTCGAAC CTGCGAGAAT 
GGCAACACGT GTCCTGGATG CCCTCTGGAG TACAGAGCGT GTAATTTGGA CTCGTGTGCT 
GAAGTGAGGC GAACGACACC GTGGACCCCC TGGTATCCTG TTAATGTGAC CCAGCTGGGA 
GCGCGGCAGG AGCAGAGGGT CCGATATACC TGTAGAGCTC TGCTGGCAGA CCCTCATGAC 
TTACAGCTCG GCAAACGCAA GATAGAGACA CGCCTCTGTC CCACCACTGA CGGAGCTGCT 
GCCTGCGAGA CTGATGGTCT GGTTGAGGAT TTGTTGAGAA TGGGTCGACC TGTAACACGA 
GTGCAAGGAG CCGCCTGGGC ATTATGGGAA ACCTGGTCTA CATGTTCAAA AGAATGTTCC 
AAAGGCTTCC GCACACGCAA ACGCTCCTGT GCCGCACCGG ATGGCAAGAG CACACCGTTC 
GCCTGCAGCG GAGCCCCGGT CGAGTATCAG GACTGCAACA CTCAACCCTG CCCAGTGAAG 
GGGGCGTGGT CATGCTGGTC TTCCTGGTCT CAGTGTTCTA CTTCCTGTGG TGGCGGTCAT 
CACCAGCGCT CCCGTTCCTG CTCGAACCCT GCGCCGGCTT ATTCTGGAGA CATCTGCATC 
GGCCTGCACA CAGAGGAGGC GCTCTGTAAC ACACACGAGT GTGAAGGTGG GTGGGGGGCG 
TGGTCAGAGT GGGCAGAGTG TAATGGGGAG GGGCTTCAGC TCCGGGCTCG TACATGTGAA 
ATAAGCCCCG CCTCCTGTCT TGGAAACTCC ACAGATCAGA GACAGTGTCG GCCCAATGAG 
AGCGCAGTGC TGTCAAAAGG ACAGAAGAGG AGCACAAACT GTGGAGGGTT TTCTCTGTTC 
CACCTGGTTC TGACTGGTGC ATGTTGTTTT CTGGGAGCCG TTTTACTCTC CATCCTGGTC 
TATGTGTACT GTCAGAGACT CCGCAAACCT CCGCAAGAAT CTGCAGTCAT CCATCCCAGC 
ACCCCAAACC ATCTCCACTA TAAAGACAAC AGCTGCGCGC CCAAGAACGA TTTATACACA 
CCCATGGAGT TCAAGACTCT CAATAAGAAC AACCTGCTTC CACCAGATGA AAGCTGCAAC 
TTCTTCCCAT CGCCCTTACC ACAGACCAAC GTCTACACCA CCACATATTA CCCGTCTCCG 
CTGGGCAAAT ACGACTTCCC TTCAATGGAT TCCCCCTGCA GCTTCATGCA CAGCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

PubMed

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SEMA5BB cDNA ORF clone, Danio rerio(Zebrafish)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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FAX

オンラインフォーム

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