LOC107090390 gene cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow)

mRNA	Protein	Name
XM_015383728.1	XP_015239214.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X2
XM_015383740.1	XP_015239226.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X12
XM_015383733.1	XP_015239219.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X6
XM_015383736.1	XP_015239222.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X9
XM_015383737.1	XP_015239223.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X10
XM_015383731.1	XP_015239217.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X5
XM_015383734.1	XP_015239220.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X7
XM_015383742.1	XP_015239228.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X14
XM_015383729.1	XP_015239215.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X3
XM_015383739.1	XP_015239225.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X11
XM_015383741.1	XP_015239227.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X13
XM_015383727.1	XP_015239213.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X1
XM_015383730.1	XP_015239216.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X4
XM_015383735.1	XP_015239221.1	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X8

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)
OCy14735	XM_015383740.1 Latest version!	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X12, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$797.30 $1139.00
OCy14736	XM_015383741.1 Latest version!	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X13, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCy14737	XM_015383742.1 Latest version!	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X14, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$713.30 $1019.00
OCy14724	XM_015383727.1 Latest version!	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCy14725	XM_015383728.1 Latest version!	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X2, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCy14726	XM_015383729.1 Latest version!	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X3, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCy14727	XM_015383730.1 Latest version!	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X4, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCy14728	XM_015383731.1 Latest version!	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X5, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCy14729	XM_015383733.1 Latest version!	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X6, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCy14730	XM_015383734.1 Latest version!	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X7, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCy14731	XM_015383735.1 Latest version!	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X8, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCy14732	XM_015383736.1 Latest version!	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X9, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCy14733	XM_015383737.1 Latest version!	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X10, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00
OCy14734	XM_015383739.1 Latest version!	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X11, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	24-26	$881.30 $1259.00

LOC107090390 ( XM_015383740.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015239226.1 Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X12, mRNA.

Price & Availability↑

CloneID	OCy14735
Clone ID Related Accession (Same CDS sequence)	XM_015383740.1
Accession Version	XM_015383740.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4215bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X12
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150746.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGCAAAAAG TCCGCGTAGT GCCTGCTGAC AACGACGATG ACGACCAGGC CACGGAAATG 
AACAACAGTT TTACAGGATC CTTAGTGCGT AGTCTCTTCC CCTCTCCTTT GCTGTGCACA 
GGTAACCCTA ACGACCAGGA CGAGAGCCGC AGCGGCATCC TCCGGCCGTA TCCCGTGCGT 
GATAATGCTC TACCCTGTGG TCTGAACAAT GCAGCCATTT CCACTGCAGA GAGCGGGCAG 
CAGACTCTTG CAGAGCCCCA GGTCTCACCA GAGGACCCAC TGGGACCGCT GCCTGAAAAC 
TGGGAGATGG CCTACACAGA GAACGGGGAA CTGTACTTCA TAGACACGAC AAAGACTCTC 
CACCAGGACA TGGCTGTGTG TTTTCAGAGC TTCCACAACA CAAAGACCAC TTCATGGCTG 
GATCCCCGGT GTAGAGACAA AGTCCCCAGG CCTCTGGAAG AGTGTGATGA TGATGAAGAA 
GGGATACATG CGGAAGACCT GAAAAGTGAT CTAGAACTGC CTCCAGGATG GGAGAGAATA 
GATGACCCAG TGTACGGAGT GTATTATGTG GACCATATAA ACAGGAAGAC CCAGTATGAG 
AACCCAGTTT TGGAGGCGCG GCATCAAAAA ATCTTGGAGC AGCAGCAACC GCCGCAGCCT 
CCAGAAGGTG AGCGGTATAT CCGAGAGTGG ATAGAGGAGC ATTCGTCTGC TGCAGCTCCA 
TTGGCAAACT ATGTTCCAAA CCACCTTGAG ACATACAGGG ACCCACAAGT CCCACCAGCT 
CTACCTCCTG GCCCAGTCGG GGTTAAAAGG GGGAAGCCTT TCTTCACCAG AAATCCTGCA 
GAACTGAAAG GAACCTTTAT CAACACCAAG CTAAAGAAAA GTCGCCGTGG ATTTGGCTTC 
ACAGTCGTTG GTGGCGATGA GCCTGATGAA TTCCTGCAGA TCAAAAGTCT GGTGCTGGAC 
GGGCCTGCAG CTGTCGATGG CAAAATGGAG ACAGGGGATG TGATTGTCAG TGTAAATGAC 
ACCATCGTTC TGGGCTACAC ACATGCACAA GTGGTGAAGA TTTTCCAGTC CATCCCTATC 
GGCTCAATGG TGGACCTGGC CTTATGTCGA GGATATCCGC TGCCATTTGA CCCAGATGAC 
CCAAACACCA GCCTAGTGAC CTCTGTGGCG ATCTTGGACA AGGAGCCTAT CATTGTCAAT 
GGACAAGAGA CTTTTGATGT ACCCGCCAAC CAGGCTGGAC CCATCAATGG CATGCGGGAG 
GCACGGTCGC ACAGCCCCTC AGCGGAGGTG GCATCAAACG GTTCACATGG GTACTCCGCT 
GACGTGGTCA CCCTGGCCTC AGCCATCGCC ACCCAGCCAG AGCTGATCAC CATACACATG 
GAGAAGGGAG ACAAAGGGTT TGGCTTCACC ATTGCTGACA GCCTGACAGG AGGCGGGCAG 
AGAGTCAAGC AGATAGTGGA CTACCCACGG TGCCGTGGCC TGAAAGAGGG GGACATTCTG 
ATGGAGGTTA ACAAGAGAAA CGTCCAGAAT ATGAGCCACA ACCAGGTGGT TGATCTGCTC 
AGCAAATGTC CGAGAGGCAG CGAAGTCACC ATGTTGGTGC AAAGAGGCGT GGTGCCAGCC 
AAGAGAAGCC CAAAGCTGGT GCACCAGTTG GAGAGAAAGG ACAGTCAGAC CAGCTCCCAG 
CACAGCGTTT GCAGCCATCG AAGTGCTCAC ACCGACTCTC CAAGCCACCC ACCCTCTGCC 
ATGCCAAGTG AGGCAGTGGT CCCCACTCCT GCTGCTCCCA CACAGCCTCT GCCGGTCTTG 
CCCCCCCAAG ACCCAGCAGA TGGCACTCTC ACCCTCCAGA AGAAGCCAGA CCCATTCAAG 
ATCTGGGCCC AGTCCAGAAG CATGTATGAA AGTCGATTGC CAGATTGCCA GGAGCAAGAC 
ATTTTCCTTT GGAGGAAGGA TACTGGCTTT GGCTTCCGCA TTTTAGGAGG AAATGAGCCT 
GGAGAACCTA TTTACATTGG TCATATAGTG AAATATGGTG CTGCGGATGA AGATGGCCGC 
CTGCGGTCTG GTGATGAGCT TATTTGTGTG GACGGCACAG CGGTAGTGGG CAAGTCCCAC 
CAGCTGGTGG TTCAGCTGAT GCAGCAGGCT GCCAAACAGG GTCATGTAAA CCTCACCGTC 
AGGCGCAAGA CCCCCGGATA TGGAGTGTCA AAGGGAGAGG GTGATGTTCC GCCATCGCCG 
GCCTCTTCCC ATCACAGCAG CACTCAGGCG CCAAGCCTCA CAGAAGGCAA GCGGACCCCT 
CAGGGAAGCC AAAACTCCCT GAATACTGTT AGCTCTGGCA GTGGATCTAC CAGCGGTATA 
GGCAGTGGTG GTGGAGGTGG CAGTGGAAGC GCTGTGGTGC CCGCCTCTCT CCAGCCATAC 
GACGTAGAGA TCCAGCGGGG AGAAAATGAG GGATTTGGTT TCGTCATCGT ATCATCCGTC 
TCCCGGCCTG ATGCTGGAAC CACATTGGCT GGAAATGCAT GCGTTGCGAT GCCCCACAAA 
ATAGGTCGTA TCATAGAGGG CAGCCCGGCT GACCGCTGCG GGAAGCTGAA GGTTGGAGAC 
CGGATTCTGG CTGTCAATGG CTGCTCCATC ACAAACAAGT CACATTCGGA CATCGTCAAC 
CTGATCAAGG AGGCCGGGAA CACTGTCTCA CTCAGGATCA TTCCAGGCGA TGAATCTTCC 
AATGCTTCTC TCCTGACTAA TGCAGAGAAA ATCGCTACCA TCACCACCAC ACACACACCT 
CAAACCACAG AGTTAAGGAA TCATTCAAAG TCAAAAGGAG TTCCTCCTCC ACCTCCTTCG 
CAAACTCATC TGCAGGATGC AGAATACTAT TCTGTGGACC TTGAACGGGA CAGTAAAGGC 
TTCGGCTTCA GCCTAAGAGG AGGACGGGAG TACAACATGG ACCTTTATGT GCTGAGACTA 
GCCGAGGATG GGGCTGCTGT CAGAAATGGC AAAATGAGGG TGGGAGATGA GATCCTGGAG 
ATTAATGGTG AGAGCACAAA GAACATGAAG CATTCACGAG CCATTGAACT GATCAAGAAT 
GGAGGTCGGA GGGCAAGGCT TGTCCTGAAG AGAGGAGATG GATCTGTACC CGAGTATGAC 
GGCAGCAGTG ACGGGTACCC TTCCCCATCC GGGGCACAAA ACGCTCCGGA AGTGAGAAAG 
CTGCCGCCCA ACAGCCGATA TCACACCTCA TTTGAGTCCA GTTATCCACC AGACTTTCAC 
AAACCATCAC GCCTGGAGGA GCCTTCGCGT GGCCGAAGTA TGGAGAAGAA CAGGGACTGG 
GCCGGCTCAG ATCTGATGGA CTTCCATGGC CGGCAGTTTA ACCCCCACCT TCATCACACC 
TGGAATAACA GTCACAGTCA AAGTATCCCC CGACAGCAGT CTCCCGACAA CAGTAATGGC 
AGCACCGTTG GCAACAAGAA GAGCCGAGGA CGCAAAGTCA AGAAGTCTGA ATCAGAGAGT 
GGCATCTCCA AGCTCCTGAA AAGTCTAAGG AAAGACAGCT CAGGGAAGAA GGCTGCTGCG 
GCCGAAAAAC TGCGAGGCAG AGAGCTCTCG AGCAGCAGTT CTACTCTGGA CAGTAAACTC 
CGTCCACAGC GTCGTGGCTC CCTTGATCGC TCACCAACCA GGAAGCGTAA CTCATCTCCG 
GATCGTCGGC GGGCAAAGTC CATGGACCGC AGAGCACATC GGGACCACAC ACCTGAGAGG 
GAATATGATG ACGGAGGGTT TCTCTCTGTA ACACCTGAGA GAAAGCTCTA TGAACGGAAG 
CTCCTCAAGG AGTCACCGTT AGCCGGTCGA GTCAGTGAGG TCTCCACCCC TGAGCGAGTC 
AACAACAGTG CAGATTCTCT CACCAGAGGC CGGGCATACG ATAAAGCCAC TAAGAACGGA 
AACCTTTTGA GAGACTCTTC CTCAGAAAGG AGGGAGGAGA GGCACAGAGG GAATGGGACA 
ATGGAGAGGC GGTACAGACT GGAGCGGGAC TCTTCCCCTG ACAGCAACTA CAGTCGGGAC 
GAGCTGAACT ATGCAGCAGC AGCAGCACCC AGACTGAAGG ACTACTACAG CATGATGAAG 
AACAATGCTG CACACAACAA CGCTACCTAC AACAAGACAG GACACAACAA TACTGCTGGA 
AGACGCAGCT CAAACCAGAT CCCTGAGCCC AAGAGAAGGC TGTACAAAGA AAACCCAAAA 
GACCTCAGTA TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015239226.1
CDS	478..4692
Translation	MQKVRVVPADNDDDDQATEMNNSFTGSLVRSLFPSPLLCTGNPNDQDESRSGILRPYPVRDNALPCGLNNAAISTAESGQQTLAEPQVSPEDPLGPLPENWEMAYTENGELYFIDTTKTLHQDMAVCFQSFHNTKTTSWLDPRCRDKVPRPLEECDDDEEGIHAEDLKSDLELPPGWERIDDPVYGVYYVDHINRKTQYENPVLEARHQKILEQQQPPQPPEGERYIREWIEEHSSAAAPLANYVPNHLETYRDPQVPPALPPGPVGVKRGKPFFTRNPAELKGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAAVDGKMETGDVIVSVNDTIVLGYTHAQVVKIFQSIPIGSMVDLALCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETFDVPANQAGPINGMREARSHSPSAEVASNGSHGYSADVVTLASAIATQPELITIHMEKGDKGFGFTIADSLTGGGQRVKQIVDYPRCRGLKEGDILMEVNKRNVQNMSHNQVVDLLSKCPRGSEVTMLVQRGVVPAKRSPKLVHQLERKDSQTSSQHSVCSHRSAHTDSPSHPPSAMPSEAVVPTPAAPTQPLPVLPPQDPADGTLTLQKKPDPFKIWAQSRSMYESRLPDCQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTPGYGVSKGEGDVPPSPASSHHSSTQAPSLTEGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGSAVVPASLQPYDVEIQRGENEGFGFVIVSSVSRPDAGTTLAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVSLRIIPGDESSNASLLTNAEKIATITTTHTPQTTELRNHSKSKGVPPPPPSQTHLQDAEYYSVDLERDSKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKNMKHSRAIELIKNGGRRARLVLKRGDGSVPEYDGSSDGYPSPSGAQNAPEVRKLPPNSRYHTSFESSYPPDFHKPSRLEEPSRGRSMEKNRDWAGSDLMDFHGRQFNPHLHHTWNNSHSQSIPRQQSPDNSNGSTVGNKKSRGRKVKKSESESGISKLLKSLRKDSSGKKAAAAEKLRGRELSSSSSTLDSKLRPQRRGSLDRSPTRKRNSSPDRRRAKSMDRRAHRDHTPEREYDDGGFLSVTPERKLYERKLLKESPLAGRVSEVSTPERVNNSADSLTRGRAYDKATKNGNLLRDSSSERREERHRGNGTMERRYRLERDSSPDSNYSRDELNYAAAAAPRLKDYYSMMKNNAAHNNATYNKTGHNNTAGRRSSNQIPEPKRRLYKENPKDLSI

Target ORF information:

RefSeq Version	XM_015383740.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X12, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015383740.1

ORF Insert Sequence:

ATGCAAAAAG TCCGCGTAGT GCCTGCTGAC AACGACGATG ACGACCAGGC CACGGAAATG 
AACAACAGTT TTACAGGATC CTTAGTGCGT AGTCTCTTCC CCTCTCCTTT GCTGTGCACA 
GGTAACCCTA ACGACCAGGA CGAGAGCCGC AGCGGCATCC TCCGGCCGTA TCCCGTGCGT 
GATAATGCTC TACCCTGTGG TCTGAACAAT GCAGCCATTT CCACTGCAGA GAGCGGGCAG 
CAGACTCTTG CAGAGCCCCA GGTCTCACCA GAGGACCCAC TGGGACCGCT GCCTGAAAAC 
TGGGAGATGG CCTACACAGA GAACGGGGAA CTGTACTTCA TAGACACGAC AAAGACTCTC 
CACCAGGACA TGGCTGTGTG TTTTCAGAGC TTCCACAACA CAAAGACCAC TTCATGGCTG 
GATCCCCGGT GTAGAGACAA AGTCCCCAGG CCTCTGGAAG AGTGTGATGA TGATGAAGAA 
GGGATACATG CGGAAGACCT GAAAAGTGAT CTAGAACTGC CTCCAGGATG GGAGAGAATA 
GATGACCCAG TGTACGGAGT GTATTATGTG GACCATATAA ACAGGAAGAC CCAGTATGAG 
AACCCAGTTT TGGAGGCGCG GCATCAAAAA ATCTTGGAGC AGCAGCAACC GCCGCAGCCT 
CCAGAAGGTG AGCGGTATAT CCGAGAGTGG ATAGAGGAGC ATTCGTCTGC TGCAGCTCCA 
TTGGCAAACT ATGTTCCAAA CCACCTTGAG ACATACAGGG ACCCACAAGT CCCACCAGCT 
CTACCTCCTG GCCCAGTCGG GGTTAAAAGG GGGAAGCCTT TCTTCACCAG AAATCCTGCA 
GAACTGAAAG GAACCTTTAT CAACACCAAG CTAAAGAAAA GTCGCCGTGG ATTTGGCTTC 
ACAGTCGTTG GTGGCGATGA GCCTGATGAA TTCCTGCAGA TCAAAAGTCT GGTGCTGGAC 
GGGCCTGCAG CTGTCGATGG CAAAATGGAG ACAGGGGATG TGATTGTCAG TGTAAATGAC 
ACCATCGTTC TGGGCTACAC ACATGCACAA GTGGTGAAGA TTTTCCAGTC CATCCCTATC 
GGCTCAATGG TGGACCTGGC CTTATGTCGA GGATATCCGC TGCCATTTGA CCCAGATGAC 
CCAAACACCA GCCTAGTGAC CTCTGTGGCG ATCTTGGACA AGGAGCCTAT CATTGTCAAT 
GGACAAGAGA CTTTTGATGT ACCCGCCAAC CAGGCTGGAC CCATCAATGG CATGCGGGAG 
GCACGGTCGC ACAGCCCCTC AGCGGAGGTG GCATCAAACG GTTCACATGG GTACTCCGCT 
GACGTGGTCA CCCTGGCCTC AGCCATCGCC ACCCAGCCAG AGCTGATCAC CATACACATG 
GAGAAGGGAG ACAAAGGGTT TGGCTTCACC ATTGCTGACA GCCTGACAGG AGGCGGGCAG 
AGAGTCAAGC AGATAGTGGA CTACCCACGG TGCCGTGGCC TGAAAGAGGG GGACATTCTG 
ATGGAGGTTA ACAAGAGAAA CGTCCAGAAT ATGAGCCACA ACCAGGTGGT TGATCTGCTC 
AGCAAATGTC CGAGAGGCAG CGAAGTCACC ATGTTGGTGC AAAGAGGCGT GGTGCCAGCC 
AAGAGAAGCC CAAAGCTGGT GCACCAGTTG GAGAGAAAGG ACAGTCAGAC CAGCTCCCAG 
CACAGCGTTT GCAGCCATCG AAGTGCTCAC ACCGACTCTC CAAGCCACCC ACCCTCTGCC 
ATGCCAAGTG AGGCAGTGGT CCCCACTCCT GCTGCTCCCA CACAGCCTCT GCCGGTCTTG 
CCCCCCCAAG ACCCAGCAGA TGGCACTCTC ACCCTCCAGA AGAAGCCAGA CCCATTCAAG 
ATCTGGGCCC AGTCCAGAAG CATGTATGAA AGTCGATTGC CAGATTGCCA GGAGCAAGAC 
ATTTTCCTTT GGAGGAAGGA TACTGGCTTT GGCTTCCGCA TTTTAGGAGG AAATGAGCCT 
GGAGAACCTA TTTACATTGG TCATATAGTG AAATATGGTG CTGCGGATGA AGATGGCCGC 
CTGCGGTCTG GTGATGAGCT TATTTGTGTG GACGGCACAG CGGTAGTGGG CAAGTCCCAC 
CAGCTGGTGG TTCAGCTGAT GCAGCAGGCT GCCAAACAGG GTCATGTAAA CCTCACCGTC 
AGGCGCAAGA CCCCCGGATA TGGAGTGTCA AAGGGAGAGG GTGATGTTCC GCCATCGCCG 
GCCTCTTCCC ATCACAGCAG CACTCAGGCG CCAAGCCTCA CAGAAGGCAA GCGGACCCCT 
CAGGGAAGCC AAAACTCCCT GAATACTGTT AGCTCTGGCA GTGGATCTAC CAGCGGTATA 
GGCAGTGGTG GTGGAGGTGG CAGTGGAAGC GCTGTGGTGC CCGCCTCTCT CCAGCCATAC 
GACGTAGAGA TCCAGCGGGG AGAAAATGAG GGATTTGGTT TCGTCATCGT ATCATCCGTC 
TCCCGGCCTG ATGCTGGAAC CACATTGGCT GGAAATGCAT GCGTTGCGAT GCCCCACAAA 
ATAGGTCGTA TCATAGAGGG CAGCCCGGCT GACCGCTGCG GGAAGCTGAA GGTTGGAGAC 
CGGATTCTGG CTGTCAATGG CTGCTCCATC ACAAACAAGT CACATTCGGA CATCGTCAAC 
CTGATCAAGG AGGCCGGGAA CACTGTCTCA CTCAGGATCA TTCCAGGCGA TGAATCTTCC 
AATGCTTCTC TCCTGACTAA TGCAGAGAAA ATCGCTACCA TCACCACCAC ACACACACCT 
CAAACCACAG AGTTAAGGAA TCATTCAAAG TCAAAAGGAG TTCCTCCTCC ACCTCCTTCG 
CAAACTCATC TGCAGGATGC AGAATACTAT TCTGTGGACC TTGAACGGGA CAGTAAAGGC 
TTCGGCTTCA GCCTAAGAGG AGGACGGGAG TACAACATGG ACCTTTATGT GCTGAGACTA 
GCCGAGGATG GGGCTGCTGT CAGAAATGGC AAAATGAGGG TGGGAGATGA GATCCTGGAG 
ATTAATGGTG AGAGCACAAA GAACATGAAG CATTCACGAG CCATTGAACT GATCAAGAAT 
GGAGGTCGGA GGGCAAGGCT TGTCCTGAAG AGAGGAGATG GATCTGTACC CGAGTATGAC 
GGCAGCAGTG ACGGGTACCC TTCCCCATCC GGGGCACAAA ACGCTCCGGA AGTGAGAAAG 
CTGCCGCCCA ACAGCCGATA TCACACCTCA TTTGAGTCCA GTTATCCACC AGACTTTCAC 
AAACCATCAC GCCTGGAGGA GCCTTCGCGT GGCCGAAGTA TGGAGAAGAA CAGGGACTGG 
GCCGGCTCAG ATCTGATGGA CTTCCATGGC CGGCAGTTTA ACCCCCACCT TCATCACACC 
TGGAATAACA GTCACAGTCA AAGTATCCCC CGACAGCAGT CTCCCGACAA CAGTAATGGC 
AGCACCGTTG GCAACAAGAA GAGCCGAGGA CGCAAAGTCA AGAAGTCTGA ATCAGAGAGT 
GGCATCTCCA AGCTCCTGAA AAGTCTAAGG AAAGACAGCT CAGGGAAGAA GGCTGCTGCG 
GCCGAAAAAC TGCGAGGCAG AGAGCTCTCG AGCAGCAGTT CTACTCTGGA CAGTAAACTC 
CGTCCACAGC GTCGTGGCTC CCTTGATCGC TCACCAACCA GGAAGCGTAA CTCATCTCCG 
GATCGTCGGC GGGCAAAGTC CATGGACCGC AGAGCACATC GGGACCACAC ACCTGAGAGG 
GAATATGATG ACGGAGGGTT TCTCTCTGTA ACACCTGAGA GAAAGCTCTA TGAACGGAAG 
CTCCTCAAGG AGTCACCGTT AGCCGGTCGA GTCAGTGAGG TCTCCACCCC TGAGCGAGTC 
AACAACAGTG CAGATTCTCT CACCAGAGGC CGGGCATACG ATAAAGCCAC TAAGAACGGA 
AACCTTTTGA GAGACTCTTC CTCAGAAAGG AGGGAGGAGA GGCACAGAGG GAATGGGACA 
ATGGAGAGGC GGTACAGACT GGAGCGGGAC TCTTCCCCTG ACAGCAACTA CAGTCGGGAC 
GAGCTGAACT ATGCAGCAGC AGCAGCACCC AGACTGAAGG ACTACTACAG CATGATGAAG 
AACAATGCTG CACACAACAA CGCTACCTAC AACAAGACAG GACACAACAA TACTGCTGGA 
AGACGCAGCT CAAACCAGAT CCCTGAGCCC AAGAGAAGGC TGTACAAAGA AAACCCAAAA 
GACCTCAGTA TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107090390 ( XM_015383741.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015239227.1 Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X13, mRNA.

Price & Availability↑

CloneID	OCy14736
Clone ID Related Accession (Same CDS sequence)	XM_015383741.1
Accession Version	XM_015383741.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3915bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X13
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150746.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACAA AAGTAAATGA GTGCACGATT 
ATCCGGGACA CGAGTGGGGA GTTGAACGTG GAGCTGCACG GTGGAGCGGA GAATGGAGAG 
TTTACATACA TTGGAGATAT CAGGGAGGAT GCTGTGGTTT ATCAGGACGG AAAGCTGTCC 
AAGGGAGAAC TACTGCTAGA CGTGGAGGGT CTGTCGGTGT CTGGATTACC CCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAGGGA CCTGTCAGGT TTAAAACTGT TCGACAAGGC 
AACAAGCTCA ACAAGGACCT AAAGCACTTC TTGAGCTTAA GGTTTCAGAA AACATCACCT 
GACCACGAGT TGCAGAAAAC CATCCGAGCC AATCTATATC GCCATGCTGT GCCTTGTACG 
ACCCGCCCCC CTCGAGACGG AGAGGTCCCA GGGTTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTGGAGGA CAGTGGCACA CTACTGGAAG TAGGAACATA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC TCCAAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
GCTGGCTCCC TTCCGTCCAC ACCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAAAGTC 
CGCGTAGTGC CTGCTGACAA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGATCCT TAGTGCGTAG TCTCTTCCCC TCTCCTTTGC TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGCAG CGGCATCCTC CGGCCGTATC CCGTGCGTGA TAATGCTCTA 
CCCTGTGGTC TGAACAATGC AGCCATTTCC ACTGCAGAGA GCGGGCAGCA GACTCTTGCA 
GAGCCCCAGG TCTCACCAGA GGACCCACTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACAGAGA ACGGGGAACT GTACTTCATA GACACGACAA AGACTCTCCA CCAGGACATG 
GCTGTGTGTT TTCAGAGCTT CCACAACACA AAGACCACTT CATGGCTGGA TCCCCGGTGT 
AGAGACAAAG TCCCCAGGCC TCTGGAAGAG TGTGATGATG ATGAAGAAGG GATACATGCG 
GAAGACCTGA AAAGTGATCT AGAACTGCCT CCAGGATGGG AGAGAATAGA TGACCCAGTG 
TACGGAGTGT ATTATGTGGA CCATATAAAC AGGAAGACCC AGTATGAGAA CCCAGTTTTG 
GAGGCGCGGC ATCAAAAAAT CTTGGAGCAG CAGCAACCGC CGCAGCCTCC AGAAGGTGAG 
CGGTATATCC GAGAGTGGAT AGAGGAGCAT TCGTCTGCTG CAGCTCCATT GGCAAACTAT 
GTTCCAAACC ACCTTGAGAC ATACAGGGAC CCACAAGTCC CACCAGCTCT ACCTCCTGGC 
CCAGTCGGGG TTAAAAGGGG GAAGCCTTTC TTCACCAGAA ATCCTGCAGA ACTGAAAGGA 
ACCTTTATCA ACACCAAGCT AAAGAAAAGT CGCCGTGGAT TTGGCTTCAC AGTCGTTGGT 
GGCGATGAGC CTGATGAATT CCTGCAGATC AAAAGTCTGG TGCTGGACGG GCCTGCAGCT 
GTCGATGGCA AAATGGAGAC AGGGGATGTG ATTGTCAGTG TAAATGACAC CATCGTTCTG 
GGCTACACAC ATGCACAAGT GGTGAAGATT TTCCAGTCCA TCCCTATCGG CTCAATGGTG 
GACCTGGCCT TATGTCGAGG ATATCCGCTG CCATTTGACC CAGATGACCC AAACACCAGC 
CTAGTGACCT CTGTGGCGAT CTTGGACAAG GAGCCTATCA TTGTCAATGG ACAAGAGACT 
TTTGATGTAC CCGCCAACCA GGCTGGACCC ATCAATGGCA TGCGGGAGGC ACGGTCGCAC 
AGCCCCTCAG CGGAGGTGGC ATCAAACGGT TCACATGGGT ACTCCGCTGA CGTGGTCACC 
CTGGCCTCAG CCATCGCCAC CCAGCCAGAG CTGATCACCA TACACATGGA GAAGGGAGAC 
AAAGGGTTTG GCTTCACCAT TGCTGACAGC CTGACAGGAG GCGGGCAGAG AGTCAAGCAG 
ATAGTGGACT ACCCACGGTG CCGTGGCCTG AAAGAGGGGG ACATTCTGAT GGAGGTTAAC 
AAGAGAAACG TCCAGAATAT GAGCCACAAC CAGGTGGTTG ATCTGCTCAG CAAATGTCCG 
AGAGGCAGCG AAGTCACCAT GTTGGTGCAA AGAGGCGTGG TGCCAGCCAA GAGAAGCCCA 
AAGCTGGTGC ACCAGTTGGA GAGAAAGGAC AGTCAGACCA GCTCCCAGCA CAGCGTTTGC 
AGCCATCGAA GTGCTCACAC CGACTCTCCA AGCCACCCAC CCTCTGCCAT GCCAAGTGAG 
GCAGTGGTCC CCACTCCTGC TGCTCCCACA CAGCCTCTGC CGGTCTTGCC CCCCCAAGAC 
CCAGCAGATG GCACTCTCAC CCTCCAGAAG AAGCCAGACC CATTCAAGAT CTGGGCCCAG 
TCCAGAAGCA TGTATGAAAG TCGATTGCCA GATTGCCAGG AGCAAGACAT TTTCCTTTGG 
AGGAAGGATA CTGGCTTTGG CTTCCGCATT TTAGGAGGAA ATGAGCCTGG AGAACCTATT 
TACATTGGTC ATATAGTGAA ATATGGTGCT GCGGATGAAG ATGGCCGCCT GCGGTCTGGT 
GATGAGCTTA TTTGTGTGGA CGGCACAGCG GTAGTGGGCA AGTCCCACCA GCTGGTGGTT 
CAGCTGATGC AGCAGGCTGC CAAACAGGGT CATGTAAACC TCACCGTCAG GCGCAAGACC 
CCCGGATATG GAGTGTCAAA GGGAGAGGGT GATGTTCCGC CATCGCCGGC CTCTTCCCAT 
CACAGCAGCA CTCAGGCGCC AAGCCTCACA GAAGGCAAGC GGACCCCTCA GGGAAGCCAA 
AACTCCCTGA ATACTGTTAG CTCTGGCAGT GGATCTACCA GCGGTATAGG CAGTGGTGGT 
GGAGGTGGCA GTGGAAGCGC TGTGGTGCCC GCCTCTCTCC AGCCATACGA CGTAGAGATC 
CAGCGGGGAG AAAATGAGGG ATTTGGTTTC GTCATCGTAT CATCCGTCTC CCGGCCTGAT 
GCTGGAACCA CATTGGCTGG AAATGCATGC GTTGCGATGC CCCACAAAAT AGGTCGTATC 
ATAGAGGGCA GCCCGGCTGA CCGCTGCGGG AAGCTGAAGG TTGGAGACCG GATTCTGGCT 
GTCAATGGCT GCTCCATCAC AAACAAGTCA CATTCGGACA TCGTCAACCT GATCAAGGAG 
GCCGGGAACA CTGTCTCACT CAGGATCATT CCAGGCGATG AATCTTCCAA TGCTTCTCTC 
CTGACTAATG CAGAGAAAAT CGCTACCATC ACCACCACAC ACACACCTCA AACCACAGAG 
TTAAGGAATC ATTCAAAGTC AAAAGGAGTT CCTCCTCCAC CTCCTTCGCA AACTCATCTG 
CAGGATGCAG AATACTATTC TGTGGACCTT GAACGGGACA GTAAAGGCTT CGGCTTCAGC 
CTAAGAGGAG GACGGGAGTA CAACATGGAC CTTTATGTGC TGAGACTAGC CGAGGATGGG 
GCTGCTGTCA GAAATGGCAA AATGAGGGTG GGAGATGAGA TCCTGGAGAT TAATGGTGAG 
AGCACAAAGA ACATGAAGCA TTCACGAGCC ATTGAACTGA TCAAGAATGG AGGTCGGAGG 
GCAAGGCTTG TCCTGAAGAG AGGAGATGGA TCTGTACCCG AGTATGCGAT GATGGCTCCT 
CATCTCGCTG TTGGTACACT AAGGAATGAC AAGATGGGAG AGCCCTGTTT CTACCTAATG 
GGCCAAAATC AGACAACGAC GGCAGCAGTG ACGGGTACCC TTCCCCATCC GGGGCACAAA 
ACGCTCCGGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015239227.1
CDS	578..4492
Translation	MAKVIQKKNHWITKVNECTIIRDTSGELNVELHGGAENGEFTYIGDIREDAVVYQDGKLSKGELLLDVEGLSVSGLPLYDIYTVINCCKGPVRFKTVRQGNKLNKDLKHFLSLRFQKTSPDHELQKTIRANLYRHAVPCTTRPPRDGEVPGLDYNFLSVEDFLQLEDSGTLLEVGTYEGNYYGTPKPPRQPIIGTVTLSDAGSLPSTPKRTKSYNDMQKVRVVPADNDDDDQATEMNNSFTGSLVRSLFPSPLLCTGNPNDQDESRSGILRPYPVRDNALPCGLNNAAISTAESGQQTLAEPQVSPEDPLGPLPENWEMAYTENGELYFIDTTKTLHQDMAVCFQSFHNTKTTSWLDPRCRDKVPRPLEECDDDEEGIHAEDLKSDLELPPGWERIDDPVYGVYYVDHINRKTQYENPVLEARHQKILEQQQPPQPPEGERYIREWIEEHSSAAAPLANYVPNHLETYRDPQVPPALPPGPVGVKRGKPFFTRNPAELKGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAAVDGKMETGDVIVSVNDTIVLGYTHAQVVKIFQSIPIGSMVDLALCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETFDVPANQAGPINGMREARSHSPSAEVASNGSHGYSADVVTLASAIATQPELITIHMEKGDKGFGFTIADSLTGGGQRVKQIVDYPRCRGLKEGDILMEVNKRNVQNMSHNQVVDLLSKCPRGSEVTMLVQRGVVPAKRSPKLVHQLERKDSQTSSQHSVCSHRSAHTDSPSHPPSAMPSEAVVPTPAAPTQPLPVLPPQDPADGTLTLQKKPDPFKIWAQSRSMYESRLPDCQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTPGYGVSKGEGDVPPSPASSHHSSTQAPSLTEGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGSAVVPASLQPYDVEIQRGENEGFGFVIVSSVSRPDAGTTLAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVSLRIIPGDESSNASLLTNAEKIATITTTHTPQTTELRNHSKSKGVPPPPPSQTHLQDAEYYSVDLERDSKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKNMKHSRAIELIKNGGRRARLVLKRGDGSVPEYAMMAPHLAVGTLRNDKMGEPCFYLMGQNQTTTAAVTGTLPHPGHKTLRK

Target ORF information:

RefSeq Version	XM_015383741.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X13, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015383741.1

ORF Insert Sequence:

ATGGCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACAA AAGTAAATGA GTGCACGATT 
ATCCGGGACA CGAGTGGGGA GTTGAACGTG GAGCTGCACG GTGGAGCGGA GAATGGAGAG 
TTTACATACA TTGGAGATAT CAGGGAGGAT GCTGTGGTTT ATCAGGACGG AAAGCTGTCC 
AAGGGAGAAC TACTGCTAGA CGTGGAGGGT CTGTCGGTGT CTGGATTACC CCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAGGGA CCTGTCAGGT TTAAAACTGT TCGACAAGGC 
AACAAGCTCA ACAAGGACCT AAAGCACTTC TTGAGCTTAA GGTTTCAGAA AACATCACCT 
GACCACGAGT TGCAGAAAAC CATCCGAGCC AATCTATATC GCCATGCTGT GCCTTGTACG 
ACCCGCCCCC CTCGAGACGG AGAGGTCCCA GGGTTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTGGAGGA CAGTGGCACA CTACTGGAAG TAGGAACATA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC TCCAAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
GCTGGCTCCC TTCCGTCCAC ACCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAAAGTC 
CGCGTAGTGC CTGCTGACAA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGATCCT TAGTGCGTAG TCTCTTCCCC TCTCCTTTGC TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGCAG CGGCATCCTC CGGCCGTATC CCGTGCGTGA TAATGCTCTA 
CCCTGTGGTC TGAACAATGC AGCCATTTCC ACTGCAGAGA GCGGGCAGCA GACTCTTGCA 
GAGCCCCAGG TCTCACCAGA GGACCCACTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACAGAGA ACGGGGAACT GTACTTCATA GACACGACAA AGACTCTCCA CCAGGACATG 
GCTGTGTGTT TTCAGAGCTT CCACAACACA AAGACCACTT CATGGCTGGA TCCCCGGTGT 
AGAGACAAAG TCCCCAGGCC TCTGGAAGAG TGTGATGATG ATGAAGAAGG GATACATGCG 
GAAGACCTGA AAAGTGATCT AGAACTGCCT CCAGGATGGG AGAGAATAGA TGACCCAGTG 
TACGGAGTGT ATTATGTGGA CCATATAAAC AGGAAGACCC AGTATGAGAA CCCAGTTTTG 
GAGGCGCGGC ATCAAAAAAT CTTGGAGCAG CAGCAACCGC CGCAGCCTCC AGAAGGTGAG 
CGGTATATCC GAGAGTGGAT AGAGGAGCAT TCGTCTGCTG CAGCTCCATT GGCAAACTAT 
GTTCCAAACC ACCTTGAGAC ATACAGGGAC CCACAAGTCC CACCAGCTCT ACCTCCTGGC 
CCAGTCGGGG TTAAAAGGGG GAAGCCTTTC TTCACCAGAA ATCCTGCAGA ACTGAAAGGA 
ACCTTTATCA ACACCAAGCT AAAGAAAAGT CGCCGTGGAT TTGGCTTCAC AGTCGTTGGT 
GGCGATGAGC CTGATGAATT CCTGCAGATC AAAAGTCTGG TGCTGGACGG GCCTGCAGCT 
GTCGATGGCA AAATGGAGAC AGGGGATGTG ATTGTCAGTG TAAATGACAC CATCGTTCTG 
GGCTACACAC ATGCACAAGT GGTGAAGATT TTCCAGTCCA TCCCTATCGG CTCAATGGTG 
GACCTGGCCT TATGTCGAGG ATATCCGCTG CCATTTGACC CAGATGACCC AAACACCAGC 
CTAGTGACCT CTGTGGCGAT CTTGGACAAG GAGCCTATCA TTGTCAATGG ACAAGAGACT 
TTTGATGTAC CCGCCAACCA GGCTGGACCC ATCAATGGCA TGCGGGAGGC ACGGTCGCAC 
AGCCCCTCAG CGGAGGTGGC ATCAAACGGT TCACATGGGT ACTCCGCTGA CGTGGTCACC 
CTGGCCTCAG CCATCGCCAC CCAGCCAGAG CTGATCACCA TACACATGGA GAAGGGAGAC 
AAAGGGTTTG GCTTCACCAT TGCTGACAGC CTGACAGGAG GCGGGCAGAG AGTCAAGCAG 
ATAGTGGACT ACCCACGGTG CCGTGGCCTG AAAGAGGGGG ACATTCTGAT GGAGGTTAAC 
AAGAGAAACG TCCAGAATAT GAGCCACAAC CAGGTGGTTG ATCTGCTCAG CAAATGTCCG 
AGAGGCAGCG AAGTCACCAT GTTGGTGCAA AGAGGCGTGG TGCCAGCCAA GAGAAGCCCA 
AAGCTGGTGC ACCAGTTGGA GAGAAAGGAC AGTCAGACCA GCTCCCAGCA CAGCGTTTGC 
AGCCATCGAA GTGCTCACAC CGACTCTCCA AGCCACCCAC CCTCTGCCAT GCCAAGTGAG 
GCAGTGGTCC CCACTCCTGC TGCTCCCACA CAGCCTCTGC CGGTCTTGCC CCCCCAAGAC 
CCAGCAGATG GCACTCTCAC CCTCCAGAAG AAGCCAGACC CATTCAAGAT CTGGGCCCAG 
TCCAGAAGCA TGTATGAAAG TCGATTGCCA GATTGCCAGG AGCAAGACAT TTTCCTTTGG 
AGGAAGGATA CTGGCTTTGG CTTCCGCATT TTAGGAGGAA ATGAGCCTGG AGAACCTATT 
TACATTGGTC ATATAGTGAA ATATGGTGCT GCGGATGAAG ATGGCCGCCT GCGGTCTGGT 
GATGAGCTTA TTTGTGTGGA CGGCACAGCG GTAGTGGGCA AGTCCCACCA GCTGGTGGTT 
CAGCTGATGC AGCAGGCTGC CAAACAGGGT CATGTAAACC TCACCGTCAG GCGCAAGACC 
CCCGGATATG GAGTGTCAAA GGGAGAGGGT GATGTTCCGC CATCGCCGGC CTCTTCCCAT 
CACAGCAGCA CTCAGGCGCC AAGCCTCACA GAAGGCAAGC GGACCCCTCA GGGAAGCCAA 
AACTCCCTGA ATACTGTTAG CTCTGGCAGT GGATCTACCA GCGGTATAGG CAGTGGTGGT 
GGAGGTGGCA GTGGAAGCGC TGTGGTGCCC GCCTCTCTCC AGCCATACGA CGTAGAGATC 
CAGCGGGGAG AAAATGAGGG ATTTGGTTTC GTCATCGTAT CATCCGTCTC CCGGCCTGAT 
GCTGGAACCA CATTGGCTGG AAATGCATGC GTTGCGATGC CCCACAAAAT AGGTCGTATC 
ATAGAGGGCA GCCCGGCTGA CCGCTGCGGG AAGCTGAAGG TTGGAGACCG GATTCTGGCT 
GTCAATGGCT GCTCCATCAC AAACAAGTCA CATTCGGACA TCGTCAACCT GATCAAGGAG 
GCCGGGAACA CTGTCTCACT CAGGATCATT CCAGGCGATG AATCTTCCAA TGCTTCTCTC 
CTGACTAATG CAGAGAAAAT CGCTACCATC ACCACCACAC ACACACCTCA AACCACAGAG 
TTAAGGAATC ATTCAAAGTC AAAAGGAGTT CCTCCTCCAC CTCCTTCGCA AACTCATCTG 
CAGGATGCAG AATACTATTC TGTGGACCTT GAACGGGACA GTAAAGGCTT CGGCTTCAGC 
CTAAGAGGAG GACGGGAGTA CAACATGGAC CTTTATGTGC TGAGACTAGC CGAGGATGGG 
GCTGCTGTCA GAAATGGCAA AATGAGGGTG GGAGATGAGA TCCTGGAGAT TAATGGTGAG 
AGCACAAAGA ACATGAAGCA TTCACGAGCC ATTGAACTGA TCAAGAATGG AGGTCGGAGG 
GCAAGGCTTG TCCTGAAGAG AGGAGATGGA TCTGTACCCG AGTATGCGAT GATGGCTCCT 
CATCTCGCTG TTGGTACACT AAGGAATGAC AAGATGGGAG AGCCCTGTTT CTACCTAATG 
GGCCAAAATC AGACAACGAC GGCAGCAGTG ACGGGTACCC TTCCCCATCC GGGGCACAAA 
ACGCTCCGGA AGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107090390 ( XM_015383742.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015239228.1 Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X14, mRNA.

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CloneID	OCy14737
Clone ID Related Accession (Same CDS sequence)	XM_015383742.1
Accession Version	XM_015383742.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3819bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X14
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150746.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACAA AAGTAAATGA GTGCACGATT 
ATCCGGGACA CGAGTGGGGA GTTGAACGTG GAGCTGCACG GTGGAGCGGA GAATGGAGAG 
TTTACATACA TTGGAGATAT CAGGGAGGAT GCTGTGGTTT ATCAGGACGG AAAGCTGTCC 
AAGGGAGAAC TACTGCTAGA CGTGGAGGGT CTGTCGGTGT CTGGATTACC CCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAGGGA CCTGTCAGGT TTAAAACTGT TCGACAAGGC 
AACAAGCTCA ACAAGGACCT AAAGCACTTC TTGAGCTTAA GGTTTCAGAA AACATCACCT 
GACCACGAGT TGCAGAAAAC CATCCGAGCC AATCTATATC GCCATGCTGT GCCTTGTACG 
ACCCGCCCCC CTCGAGACGG AGAGGTCCCA GGGTTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTGGAGGA CAGTGGCACA CTACTGGAAG TAGGAACATA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC TCCAAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
GCTGGCTCCC TTCCGTCCAC ACCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAAAGTC 
CGCGTAGTGC CTGCTGACAA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGTAACC CTAACGACCA GGACGAGAGC CGCAGCGGCA TCCTCCGGCC GTATCCCGTG 
CGTGATAATG CTCTACCCTG TGGTCTGAAC AATGCAGCCA TTTCCACTGC AGAGAGCGGG 
CAGCAGACTC TTGCAGAGCC CCAGGTCTCA CCAGAGGACC CACTGGGACC GCTGCCTGAA 
AACTGGGAGA TGGCCTACAC AGAGAACGGG GAACTGTACT TCATAGACCA CAACACAAAG 
ACCACTTCAT GGCTGGATCC CCGGTGTAGA GACAAAGTCC CCAGGCCTCT GGAAGAGTGT 
GATGATGATG AAGGGATACA TGCGGAAGAC CTGAAAAGTG ATCTAGAACT GCCTCCAGGA 
TGGGAGAGAA TAGATGACCC AGTGTACGGA GTGTATTATG TGGACCATAT AAACAGGAAG 
ACCCAGTATG AGAACCCAGT TTTGGAGGCG CGGCATCAAA AAATCTTGGA GCAGCAGCAA 
CCGCCGCAGC CTCCAGAAGG TGAGCGGTAT ATCCGAGAGT GGATAGAGGA GCATTCGTCT 
GCTGCAGCTC CATTGGCAAA CTATGTTCCA AACCACCTTG AGACATACAG GGACCCACAA 
GTCCCACCAG CTCTACCTCC TGGCCCAGTC GGGGTTAAAA GGGGGAAGCC TTTCTTCACC 
AGAAATCCTG CAGAACTGAA AGGAACCTTT ATCAACACCA AGCTAAAGAA AAGTCGCCGT 
GGATTTGGCT TCACAGTCGT TGGTGGCGAT GAGCCTGATG AATTCCTGCA GATCAAAAGT 
CTGGTGCTGG ACGGGCCTGC AGCTGTCGAT GGCAAAATGG AGACAGGGGA TGTGATTGTC 
AGTGTAAATG ACACCATCGT TCTGGGCTAC ACACATGCAC AAGTGGTGAA GATTTTCCAG 
TCCATCCCTA TCGGCTCAAT GGTGGACCTG GCCTTATGTC GAGGATATCC GCTGCCATTT 
GACCCAGATG ACCCAAACAC CAGCCTAGTG ACCTCTGTGG CGATCTTGGA CAAGGAGCCT 
ATCATTGTCA ATGGACAAGA GACTTTTGAT GTACCCGCCA ACCAGGCTGG ACCCATCAAT 
GGCATGCGGG AGGCACGGTC GCACAGCCCC TCAGCGGAGG TGGCATCAAA CGGTTCACAT 
GGGTACTCCG CTGACGTGGT CACCCTGGCC TCAGCCATCG CCACCCAGCC AGAGCTGATC 
ACCATACACA TGGAGAAGGG AGACAAAGGG TTTGGCTTCA CCATTGCTGA CAGCCTGACA 
GGAGGCGGGC AGAGAGTCAA GCAGATAGTG GACTACCCAC GGTGCCGTGG CCTGAAAGAG 
GGGGACATTC TGATGGAGGT TAACAAGAGA AACGTCCAGA ATATGAGCCA CAACCAGGTG 
GTTGATCTGC TCAGCAAATG TCCGAGAGGC AGCGAAGTCA CCATGTTGGT GCAAAGAGGC 
GTGGTGCCAG CCAAGAGAAG CCCAAAGCTG GTGCACCAGT TGGAGAGAAA GGACAGTCAG 
ACCAGCTCCC AGCACAGCGT TTGCAGCCAT CGAAGTGCTC ACACCGACTC TCCAAGCCAC 
CCACCCTCTG CCATGCCAAG TGAGGCAGTG GTCCCCACTC CTGCTGCTCC CACACAGCCT 
CTGCCGGTCT TGCCCCCCCA AGACCCAGCA GATGGCACTC TCACCCTCCA GAAGAAGCCA 
GACCCATTCA AGATCTGGGC CCAGTCCAGA AGCATGTATG AAAGTCGATT GCCAGATTGC 
CAGGAGCAAG ACATTTTCCT TTGGAGGAAG GATACTGGCT TTGGCTTCCG CATTTTAGGA 
GGAAATGAGC CTGGAGAACC TATTTACATT GGTCATATAG TGAAATATGG TGCTGCGGAT 
GAAGATGGCC GCCTGCGGTC TGGTGATGAG CTTATTTGTG TGGACGGCAC AGCGGTAGTG 
GGCAAGTCCC ACCAGCTGGT GGTTCAGCTG ATGCAGCAGG CTGCCAAACA GGGTCATGTA 
AACCTCACCG TCAGGCGCAA GACCCCCGGA TATGGAGTGT CAAAGGGAGA GGGTGATGTT 
CCGCCATCGC CGGCCTCTTC CCATCACAGC AGCACTCAGG CGCCAAGCCT CACAGAAGGC 
AAGCGGACCC CTCAGGGAAG CCAAAACTCC CTGAATACTG TTAGCTCTGG CAGTGGATCT 
ACCAGCGGTA TAGGCAGTGG TGGTGGAGGT GGCAGTGGAA GCGCTGTGGT GCCCGCCTCT 
CTCCAGCCAT ACGACGTAGA GATCCAGCGG GGAGAAAATG AGGGATTTGG TTTCGTCATC 
GTATCATCCG TCTCCCGGCC TGATGCTGGA ACCACATTGG CTGGAAATGC ATGCGTTGCG 
ATGCCCCACA AAATAGGTCG TATCATAGAG GGCAGCCCGG CTGACCGCTG CGGGAAGCTG 
AAGGTTGGAG ACCGGATTCT GGCTGTCAAT GGCTGCTCCA TCACAAACAA GTCACATTCG 
GACATCGTCA ACCTGATCAA GGAGGCCGGG AACACTGTCT CACTCAGGAT CATTCCAGGC 
GATGAATCTT CCAATGCTTC TCTCCTGACT AATGCAGAGA AAATCGCTAC CATCACCACC 
ACACACACAC CTCAAACCAC AGAGTTAAGG AATCATTCAA AGTCAAAAGG AGTTCCTCCT 
CCACCTCCTT CGCAAACTCA TCTGCAGGAT GCAGAATACT ATTCTGTGGA CCTTGAACGG 
GACAGTAAAG GCTTCGGCTT CAGCCTAAGA GGAGGACGGG AGTACAACAT GGACCTTTAT 
GTGCTGAGAC TAGCCGAGGA TGGGGCTGCT GTCAGAAATG GCAAAATGAG GGTGGGAGAT 
GAGATCCTGG AGATTAATGG TGAGAGCACA AAGAACATGA AGCATTCACG AGCCATTGAA 
CTGATCAAGA ATGGAGGTCG GAGGGCAAGG CTTGTCCTGA AGAGAGGAGA TGGATCTGTA 
CCCGAGTATG CGATGATGGC TCCTCATCTC GCTGTTGGTA CACTAAGGAA TGACAAGATG 
GGAGAGCCCT GTTTCTACCT AATGGGCCAA AATCAGACAA CGACGGCAGC AGTGACGGGT 
ACCCTTCCCC ATCCGGGGCA CAAAACGCTC CGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015239228.1
CDS	575..4393
Translation	MAKVIQKKNHWITKVNECTIIRDTSGELNVELHGGAENGEFTYIGDIREDAVVYQDGKLSKGELLLDVEGLSVSGLPLYDIYTVINCCKGPVRFKTVRQGNKLNKDLKHFLSLRFQKTSPDHELQKTIRANLYRHAVPCTTRPPRDGEVPGLDYNFLSVEDFLQLEDSGTLLEVGTYEGNYYGTPKPPRQPIIGTVTLSDAGSLPSTPKRTKSYNDMQKVRVVPADNDDDDQATEMNNSFTGNPNDQDESRSGILRPYPVRDNALPCGLNNAAISTAESGQQTLAEPQVSPEDPLGPLPENWEMAYTENGELYFIDHNTKTTSWLDPRCRDKVPRPLEECDDDEGIHAEDLKSDLELPPGWERIDDPVYGVYYVDHINRKTQYENPVLEARHQKILEQQQPPQPPEGERYIREWIEEHSSAAAPLANYVPNHLETYRDPQVPPALPPGPVGVKRGKPFFTRNPAELKGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAAVDGKMETGDVIVSVNDTIVLGYTHAQVVKIFQSIPIGSMVDLALCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETFDVPANQAGPINGMREARSHSPSAEVASNGSHGYSADVVTLASAIATQPELITIHMEKGDKGFGFTIADSLTGGGQRVKQIVDYPRCRGLKEGDILMEVNKRNVQNMSHNQVVDLLSKCPRGSEVTMLVQRGVVPAKRSPKLVHQLERKDSQTSSQHSVCSHRSAHTDSPSHPPSAMPSEAVVPTPAAPTQPLPVLPPQDPADGTLTLQKKPDPFKIWAQSRSMYESRLPDCQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTPGYGVSKGEGDVPPSPASSHHSSTQAPSLTEGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGSAVVPASLQPYDVEIQRGENEGFGFVIVSSVSRPDAGTTLAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVSLRIIPGDESSNASLLTNAEKIATITTTHTPQTTELRNHSKSKGVPPPPPSQTHLQDAEYYSVDLERDSKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKNMKHSRAIELIKNGGRRARLVLKRGDGSVPEYAMMAPHLAVGTLRNDKMGEPCFYLMGQNQTTTAAVTGTLPHPGHKTLRK

Target ORF information:

RefSeq Version	XM_015383742.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X14, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015383742.1

ORF Insert Sequence:

ATGGCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACAA AAGTAAATGA GTGCACGATT 
ATCCGGGACA CGAGTGGGGA GTTGAACGTG GAGCTGCACG GTGGAGCGGA GAATGGAGAG 
TTTACATACA TTGGAGATAT CAGGGAGGAT GCTGTGGTTT ATCAGGACGG AAAGCTGTCC 
AAGGGAGAAC TACTGCTAGA CGTGGAGGGT CTGTCGGTGT CTGGATTACC CCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAGGGA CCTGTCAGGT TTAAAACTGT TCGACAAGGC 
AACAAGCTCA ACAAGGACCT AAAGCACTTC TTGAGCTTAA GGTTTCAGAA AACATCACCT 
GACCACGAGT TGCAGAAAAC CATCCGAGCC AATCTATATC GCCATGCTGT GCCTTGTACG 
ACCCGCCCCC CTCGAGACGG AGAGGTCCCA GGGTTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTGGAGGA CAGTGGCACA CTACTGGAAG TAGGAACATA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC TCCAAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
GCTGGCTCCC TTCCGTCCAC ACCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAAAGTC 
CGCGTAGTGC CTGCTGACAA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGTAACC CTAACGACCA GGACGAGAGC CGCAGCGGCA TCCTCCGGCC GTATCCCGTG 
CGTGATAATG CTCTACCCTG TGGTCTGAAC AATGCAGCCA TTTCCACTGC AGAGAGCGGG 
CAGCAGACTC TTGCAGAGCC CCAGGTCTCA CCAGAGGACC CACTGGGACC GCTGCCTGAA 
AACTGGGAGA TGGCCTACAC AGAGAACGGG GAACTGTACT TCATAGACCA CAACACAAAG 
ACCACTTCAT GGCTGGATCC CCGGTGTAGA GACAAAGTCC CCAGGCCTCT GGAAGAGTGT 
GATGATGATG AAGGGATACA TGCGGAAGAC CTGAAAAGTG ATCTAGAACT GCCTCCAGGA 
TGGGAGAGAA TAGATGACCC AGTGTACGGA GTGTATTATG TGGACCATAT AAACAGGAAG 
ACCCAGTATG AGAACCCAGT TTTGGAGGCG CGGCATCAAA AAATCTTGGA GCAGCAGCAA 
CCGCCGCAGC CTCCAGAAGG TGAGCGGTAT ATCCGAGAGT GGATAGAGGA GCATTCGTCT 
GCTGCAGCTC CATTGGCAAA CTATGTTCCA AACCACCTTG AGACATACAG GGACCCACAA 
GTCCCACCAG CTCTACCTCC TGGCCCAGTC GGGGTTAAAA GGGGGAAGCC TTTCTTCACC 
AGAAATCCTG CAGAACTGAA AGGAACCTTT ATCAACACCA AGCTAAAGAA AAGTCGCCGT 
GGATTTGGCT TCACAGTCGT TGGTGGCGAT GAGCCTGATG AATTCCTGCA GATCAAAAGT 
CTGGTGCTGG ACGGGCCTGC AGCTGTCGAT GGCAAAATGG AGACAGGGGA TGTGATTGTC 
AGTGTAAATG ACACCATCGT TCTGGGCTAC ACACATGCAC AAGTGGTGAA GATTTTCCAG 
TCCATCCCTA TCGGCTCAAT GGTGGACCTG GCCTTATGTC GAGGATATCC GCTGCCATTT 
GACCCAGATG ACCCAAACAC CAGCCTAGTG ACCTCTGTGG CGATCTTGGA CAAGGAGCCT 
ATCATTGTCA ATGGACAAGA GACTTTTGAT GTACCCGCCA ACCAGGCTGG ACCCATCAAT 
GGCATGCGGG AGGCACGGTC GCACAGCCCC TCAGCGGAGG TGGCATCAAA CGGTTCACAT 
GGGTACTCCG CTGACGTGGT CACCCTGGCC TCAGCCATCG CCACCCAGCC AGAGCTGATC 
ACCATACACA TGGAGAAGGG AGACAAAGGG TTTGGCTTCA CCATTGCTGA CAGCCTGACA 
GGAGGCGGGC AGAGAGTCAA GCAGATAGTG GACTACCCAC GGTGCCGTGG CCTGAAAGAG 
GGGGACATTC TGATGGAGGT TAACAAGAGA AACGTCCAGA ATATGAGCCA CAACCAGGTG 
GTTGATCTGC TCAGCAAATG TCCGAGAGGC AGCGAAGTCA CCATGTTGGT GCAAAGAGGC 
GTGGTGCCAG CCAAGAGAAG CCCAAAGCTG GTGCACCAGT TGGAGAGAAA GGACAGTCAG 
ACCAGCTCCC AGCACAGCGT TTGCAGCCAT CGAAGTGCTC ACACCGACTC TCCAAGCCAC 
CCACCCTCTG CCATGCCAAG TGAGGCAGTG GTCCCCACTC CTGCTGCTCC CACACAGCCT 
CTGCCGGTCT TGCCCCCCCA AGACCCAGCA GATGGCACTC TCACCCTCCA GAAGAAGCCA 
GACCCATTCA AGATCTGGGC CCAGTCCAGA AGCATGTATG AAAGTCGATT GCCAGATTGC 
CAGGAGCAAG ACATTTTCCT TTGGAGGAAG GATACTGGCT TTGGCTTCCG CATTTTAGGA 
GGAAATGAGC CTGGAGAACC TATTTACATT GGTCATATAG TGAAATATGG TGCTGCGGAT 
GAAGATGGCC GCCTGCGGTC TGGTGATGAG CTTATTTGTG TGGACGGCAC AGCGGTAGTG 
GGCAAGTCCC ACCAGCTGGT GGTTCAGCTG ATGCAGCAGG CTGCCAAACA GGGTCATGTA 
AACCTCACCG TCAGGCGCAA GACCCCCGGA TATGGAGTGT CAAAGGGAGA GGGTGATGTT 
CCGCCATCGC CGGCCTCTTC CCATCACAGC AGCACTCAGG CGCCAAGCCT CACAGAAGGC 
AAGCGGACCC CTCAGGGAAG CCAAAACTCC CTGAATACTG TTAGCTCTGG CAGTGGATCT 
ACCAGCGGTA TAGGCAGTGG TGGTGGAGGT GGCAGTGGAA GCGCTGTGGT GCCCGCCTCT 
CTCCAGCCAT ACGACGTAGA GATCCAGCGG GGAGAAAATG AGGGATTTGG TTTCGTCATC 
GTATCATCCG TCTCCCGGCC TGATGCTGGA ACCACATTGG CTGGAAATGC ATGCGTTGCG 
ATGCCCCACA AAATAGGTCG TATCATAGAG GGCAGCCCGG CTGACCGCTG CGGGAAGCTG 
AAGGTTGGAG ACCGGATTCT GGCTGTCAAT GGCTGCTCCA TCACAAACAA GTCACATTCG 
GACATCGTCA ACCTGATCAA GGAGGCCGGG AACACTGTCT CACTCAGGAT CATTCCAGGC 
GATGAATCTT CCAATGCTTC TCTCCTGACT AATGCAGAGA AAATCGCTAC CATCACCACC 
ACACACACAC CTCAAACCAC AGAGTTAAGG AATCATTCAA AGTCAAAAGG AGTTCCTCCT 
CCACCTCCTT CGCAAACTCA TCTGCAGGAT GCAGAATACT ATTCTGTGGA CCTTGAACGG 
GACAGTAAAG GCTTCGGCTT CAGCCTAAGA GGAGGACGGG AGTACAACAT GGACCTTTAT 
GTGCTGAGAC TAGCCGAGGA TGGGGCTGCT GTCAGAAATG GCAAAATGAG GGTGGGAGAT 
GAGATCCTGG AGATTAATGG TGAGAGCACA AAGAACATGA AGCATTCACG AGCCATTGAA 
CTGATCAAGA ATGGAGGTCG GAGGGCAAGG CTTGTCCTGA AGAGAGGAGA TGGATCTGTA 
CCCGAGTATG CGATGATGGC TCCTCATCTC GCTGTTGGTA CACTAAGGAA TGACAAGATG 
GGAGAGCCCT GTTTCTACCT AATGGGCCAA AATCAGACAA CGACGGCAGC AGTGACGGGT 
ACCCTTCCCC ATCCGGGGCA CAAAACGCTC CGGAAGTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107090390 ( XM_015383727.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015239213.1 Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X1, mRNA.

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CloneID	OCy14724
Clone ID Related Accession (Same CDS sequence)	XM_015383727.1
Accession Version	XM_015383727.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4863bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150746.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACAA AAGTAAATGA GTGCACGATT 
ATCCGGGACA CGAGTGGGGA GTTGAACGTG GAGCTGCACG GTGGAGCGGA GAATGGAGAG 
TTTACATACA TTGGAGATAT CAGGGAGGAT GCTGTGGTTT ATCAGGACGG AAAGCTGTCC 
AAGGGAGAAC TACTGCTAGA CGTGGAGGGT CTGTCGGTGT CTGGATTACC CCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAGGGA CCTGTCAGGT TTAAAACTGT TCGACAAGGC 
AACAAGCTCA ACAAGGACCT AAAGCACTTC TTGAGCTTAA GGTTTCAGAA AACATCACCT 
GACCACGAGT TGCAGAAAAC CATCCGAGCC AATCTATATC GCCATGCTGT GCCTTGTACG 
ACCCGCCCCC CTCGAGACGG AGAGGTCCCA GGGTTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTGGAGGA CAGTGGCACA CTACTGGAAG TAGGAACATA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC TCCAAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
GCTGGCTCCC TTCCGTCCAC ACCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAAAGTC 
CGCGTAGTGC CTGCTGACAA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGATCCT TAGTGCGTAG TCTCTTCCCC TCTCCTTTGC TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGCAG CGGCATCCTC CGGCCGTATC CCGTGCGTGA TAATGCTCTA 
CCCTGTGGTC TGAACAATGC AGCCATTTCC ACTGCAGAGA GCGGGCAGCA GACTCTTGCA 
GAGCCCCAGG TCTCACCAGA GGACCCACTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACAGAGA ACGGGGAACT GTACTTCATA GACACGACAA AGACTCTCCA CCAGGACATG 
GCTGTGTGTT TTCAGAGCTT CCACAACACA AAGACCACTT CATGGCTGGA TCCCCGGTGT 
AGAGACAAAG TCCCCAGGCC TCTGGAAGAG TGTGATGATG ATGAAGAAGG GATACATGCG 
GAAGACCTGA AAAGTGATCT AGAACTGCCT CCAGGATGGG AGAGAATAGA TGACCCAGTG 
TACGGAGTGT ATTATGTGGA CCATATAAAC AGGAAGACCC AGTATGAGAA CCCAGTTTTG 
GAGGCGCGGC ATCAAAAAAT CTTGGAGCAG CAGCAACCGC CGCAGCCTCC AGAAGGTGAG 
CGGTATATCC GAGAGTGGAT AGAGGAGCAT TCGTCTGCTG CAGCTCCATT GGCAAACTAT 
GTTCCAAACC ACCTTGAGAC ATACAGGGAC CCACAAGTCC CACCAGCTCT ACCTCCTGGC 
CCAGTCGGGG TTAAAAGGGG GAAGCCTTTC TTCACCAGAA ATCCTGCAGA ACTGAAAGGA 
ACCTTTATCA ACACCAAGCT AAAGAAAAGT CGCCGTGGAT TTGGCTTCAC AGTCGTTGGT 
GGCGATGAGC CTGATGAATT CCTGCAGATC AAAAGTCTGG TGCTGGACGG GCCTGCAGCT 
GTCGATGGCA AAATGGAGAC AGGGGATGTG ATTGTCAGTG TAAATGACAC CATCGTTCTG 
GGCTACACAC ATGCACAAGT GGTGAAGATT TTCCAGTCCA TCCCTATCGG CTCAATGGTG 
GACCTGGCCT TATGTCGAGG ATATCCGCTG CCATTTGACC CAGATGACCC AAACACCAGC 
CTAGTGACCT CTGTGGCGAT CTTGGACAAG GAGCCTATCA TTGTCAATGG ACAAGAGACT 
TTTGATGTAC CCGCCAACCA GGCTGGACCC ATCAATGGCA TGCGGGAGGC ACGGTCGCAC 
AGCCCCTCAG CGGAGGTGGC ATCAAACGGT TCACATGGGT ACTCCGCTGA CGTGGTCACC 
CTGGCCTCAG CCATCGCCAC CCAGCCAGAG CTGATCACCA TACACATGGA GAAGGGAGAC 
AAAGGGTTTG GCTTCACCAT TGCTGACAGC CTGACAGGAG GCGGGCAGAG AGTCAAGCAG 
ATAGTGGACT ACCCACGGTG CCGTGGCCTG AAAGAGGGGG ACATTCTGAT GGAGGTTAAC 
AAGAGAAACG TCCAGAATAT GAGCCACAAC CAGGTGGTTG ATCTGCTCAG CAAATGTCCG 
AGAGGCAGCG AAGTCACCAT GTTGGTGCAA AGAGGCGTGG TGCCAGCCAA GAGAAGCCCA 
AAGCTGGTGC ACCAGTTGGA GAGAAAGGAC AGTCAGACCA GCTCCCAGCA CAGCGTTTGC 
AGCCATCGAA GTGCTCACAC CGACTCTCCA AGCCACCCAC CCTCTGCCAT GCCAAGTGAG 
GCAGTGGTCC CCACTCCTGC TGCTCCCACA CAGCCTCTGC CGGTCTTGCC CCCCCAAGAC 
CCAGCAGATG GCACTCTCAC CCTCCAGAAG AAGCCAGACC CATTCAAGAT CTGGGCCCAG 
TCCAGAAGCA TGTATGAAAG TCGATTGCCA GATTGCCAGG AGCAAGACAT TTTCCTTTGG 
AGGAAGGATA CTGGCTTTGG CTTCCGCATT TTAGGAGGAA ATGAGCCTGG AGAACCTATT 
TACATTGGTC ATATAGTGAA ATATGGTGCT GCGGATGAAG ATGGCCGCCT GCGGTCTGGT 
GATGAGCTTA TTTGTGTGGA CGGCACAGCG GTAGTGGGCA AGTCCCACCA GCTGGTGGTT 
CAGCTGATGC AGCAGGCTGC CAAACAGGGT CATGTAAACC TCACCGTCAG GCGCAAGACC 
CCCGGATATG GAGTGTCAAA GGGAGAGGGT GATGTTCCGC CATCGCCGGC CTCTTCCCAT 
CACAGCAGCA CTCAGGCGCC AAGCCTCACA GAAGGCAAGC GGACCCCTCA GGGAAGCCAA 
AACTCCCTGA ATACTGTTAG CTCTGGCAGT GGATCTACCA GCGGTATAGG CAGTGGTGGT 
GGAGGTGGCA GTGGAAGCGC TGTGGTGCCC GCCTCTCTCC AGCCATACGA CGTAGAGATC 
CAGCGGGGAG AAAATGAGGG ATTTGGTTTC GTCATCGTAT CATCCGTCTC CCGGCCTGAT 
GCTGGAACCA CATTGGCTGG AAATGCATGC GTTGCGATGC CCCACAAAAT AGGTCGTATC 
ATAGAGGGCA GCCCGGCTGA CCGCTGCGGG AAGCTGAAGG TTGGAGACCG GATTCTGGCT 
GTCAATGGCT GCTCCATCAC AAACAAGTCA CATTCGGACA TCGTCAACCT GATCAAGGAG 
GCCGGGAACA CTGTCTCACT CAGGATCATT CCAGGCGATG AATCTTCCAA TGCTTCTCTC 
CTGACTAATG CAGAGAAAAT CGCTACCATC ACCACCACAC ACACACCTCA AACCACAGAG 
TTAAGGAATC ATTCAAAGTC AAAAGGAGTT CCTCCTCCAC CTCCTTCGCA AACTCATCTG 
CAGGATGCAG AATACTATTC TGTGGACCTT GAACGGGACA GTAAAGGCTT CGGCTTCAGC 
CTAAGAGGAG GACGGGAGTA CAACATGGAC CTTTATGTGC TGAGACTAGC CGAGGATGGG 
GCTGCTGTCA GAAATGGCAA AATGAGGGTG GGAGATGAGA TCCTGGAGAT TAATGGTGAG 
AGCACAAAGA ACATGAAGCA TTCACGAGCC ATTGAACTGA TCAAGAATGG AGGTCGGAGG 
GCAAGGCTTG TCCTGAAGAG AGGAGATGGA TCTGTACCCG AGTATGACGG CAGCAGTGAC 
GGGTACCCTT CCCCATCCGG GGCACAAAAC GCTCCGGAAG TGAGAAAGCT GCCGCCCAAC 
AGCCGATATC ACACCTCATT TGAGTCCAGT TATCCACCAG ACTTTCACAA ACCATCACGC 
CTGGAGGAGC CTTCGCGTGG CCGAAGTATG GAGAAGAACA GGGACTGGGC CGGCTCAGAT 
CTGATGGACT TCCATGGCCG GCAGTTTAAC CCCCACCTTC ATCACACCTG GAATAACAGT 
CACAGTCAAA GTATCCCCCG ACAGCAGTCT CCCGACAACA GTAATGGCAG CACCGTTGGC 
AACAAGAAGA GCCGAGGACG CAAAGTCAAG AAGTCTGAAT CAGAGAGTGG CATCTCCAAG 
CTCCTGAAAA GTCTAAGGAA AGACAGCTCA GGGAAGAAGG CTGCTGCGGC CGAAAAACTG 
CGAGGCAGAG AGCTCTCGAG CAGCAGTTCT ACTCTGGACA GTAAACTCCG TCCACAGCGT 
CGTGGCTCCC TTGATCGCTC ACCAACCAGG AAGCGTAACT CATCTCCGGA TCGTCGGCGG 
GCAAAGTCCA TGGACCGCAG AGCACATCGG GACCACACAC CTGAGAGGGA ATATGATGAC 
GGAGGGTTTC TCTCTGTAAC ACCTGAGAGA AAGCTCTATG AACGGAAGCT CCTCAAGGAG 
TCACCGTTAG CCGGTCGAGT CAGTGAGGTC TCCACCCCTG AGCGAGTCAA CAACAGTGCA 
GATTCTCTCA CCAGAGGCCG GGCATACGAT AAAGCCACTA AGAACGGAAA CCTTTTGAGA 
GACTCTTCCT CAGAAAGGAG GGAGGAGAGG CACAGAGGGA ATGGGACAAT GGAGAGGCGG 
TACAGACTGG AGCGGGACTC TTCCCCTGAC AGCAACTACA GTCGGGACGA GCTGAACTAT 
GCAGCAGCAG CAGCACCCAG ACTGAAGGAC TACTACAGCA TGATGAAGAA CAATGCTGCA 
CACAACAACG CTACCTACAA CAAGACAGGA CACAACAATA CTGCTGGAAG ACGCAGCTCA 
AACCAGATCC CTGAGCCCAA GAGAAGGCTG TACAAAGAAA ACCCAAAAGA CCTCAGTATC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015239213.1
CDS	575..5437
Translation	MAKVIQKKNHWITKVNECTIIRDTSGELNVELHGGAENGEFTYIGDIREDAVVYQDGKLSKGELLLDVEGLSVSGLPLYDIYTVINCCKGPVRFKTVRQGNKLNKDLKHFLSLRFQKTSPDHELQKTIRANLYRHAVPCTTRPPRDGEVPGLDYNFLSVEDFLQLEDSGTLLEVGTYEGNYYGTPKPPRQPIIGTVTLSDAGSLPSTPKRTKSYNDMQKVRVVPADNDDDDQATEMNNSFTGSLVRSLFPSPLLCTGNPNDQDESRSGILRPYPVRDNALPCGLNNAAISTAESGQQTLAEPQVSPEDPLGPLPENWEMAYTENGELYFIDTTKTLHQDMAVCFQSFHNTKTTSWLDPRCRDKVPRPLEECDDDEEGIHAEDLKSDLELPPGWERIDDPVYGVYYVDHINRKTQYENPVLEARHQKILEQQQPPQPPEGERYIREWIEEHSSAAAPLANYVPNHLETYRDPQVPPALPPGPVGVKRGKPFFTRNPAELKGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAAVDGKMETGDVIVSVNDTIVLGYTHAQVVKIFQSIPIGSMVDLALCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETFDVPANQAGPINGMREARSHSPSAEVASNGSHGYSADVVTLASAIATQPELITIHMEKGDKGFGFTIADSLTGGGQRVKQIVDYPRCRGLKEGDILMEVNKRNVQNMSHNQVVDLLSKCPRGSEVTMLVQRGVVPAKRSPKLVHQLERKDSQTSSQHSVCSHRSAHTDSPSHPPSAMPSEAVVPTPAAPTQPLPVLPPQDPADGTLTLQKKPDPFKIWAQSRSMYESRLPDCQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTPGYGVSKGEGDVPPSPASSHHSSTQAPSLTEGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGSAVVPASLQPYDVEIQRGENEGFGFVIVSSVSRPDAGTTLAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVSLRIIPGDESSNASLLTNAEKIATITTTHTPQTTELRNHSKSKGVPPPPPSQTHLQDAEYYSVDLERDSKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKNMKHSRAIELIKNGGRRARLVLKRGDGSVPEYDGSSDGYPSPSGAQNAPEVRKLPPNSRYHTSFESSYPPDFHKPSRLEEPSRGRSMEKNRDWAGSDLMDFHGRQFNPHLHHTWNNSHSQSIPRQQSPDNSNGSTVGNKKSRGRKVKKSESESGISKLLKSLRKDSSGKKAAAAEKLRGRELSSSSSTLDSKLRPQRRGSLDRSPTRKRNSSPDRRRAKSMDRRAHRDHTPEREYDDGGFLSVTPERKLYERKLLKESPLAGRVSEVSTPERVNNSADSLTRGRAYDKATKNGNLLRDSSSERREERHRGNGTMERRYRLERDSSPDSNYSRDELNYAAAAAPRLKDYYSMMKNNAAHNNATYNKTGHNNTAGRRSSNQIPEPKRRLYKENPKDLSI

Target ORF information:

RefSeq Version	XM_015383727.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015383727.1

ORF Insert Sequence:

ATGGCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACAA AAGTAAATGA GTGCACGATT 
ATCCGGGACA CGAGTGGGGA GTTGAACGTG GAGCTGCACG GTGGAGCGGA GAATGGAGAG 
TTTACATACA TTGGAGATAT CAGGGAGGAT GCTGTGGTTT ATCAGGACGG AAAGCTGTCC 
AAGGGAGAAC TACTGCTAGA CGTGGAGGGT CTGTCGGTGT CTGGATTACC CCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAGGGA CCTGTCAGGT TTAAAACTGT TCGACAAGGC 
AACAAGCTCA ACAAGGACCT AAAGCACTTC TTGAGCTTAA GGTTTCAGAA AACATCACCT 
GACCACGAGT TGCAGAAAAC CATCCGAGCC AATCTATATC GCCATGCTGT GCCTTGTACG 
ACCCGCCCCC CTCGAGACGG AGAGGTCCCA GGGTTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTGGAGGA CAGTGGCACA CTACTGGAAG TAGGAACATA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC TCCAAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
GCTGGCTCCC TTCCGTCCAC ACCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAAAGTC 
CGCGTAGTGC CTGCTGACAA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGATCCT TAGTGCGTAG TCTCTTCCCC TCTCCTTTGC TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGCAG CGGCATCCTC CGGCCGTATC CCGTGCGTGA TAATGCTCTA 
CCCTGTGGTC TGAACAATGC AGCCATTTCC ACTGCAGAGA GCGGGCAGCA GACTCTTGCA 
GAGCCCCAGG TCTCACCAGA GGACCCACTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACAGAGA ACGGGGAACT GTACTTCATA GACACGACAA AGACTCTCCA CCAGGACATG 
GCTGTGTGTT TTCAGAGCTT CCACAACACA AAGACCACTT CATGGCTGGA TCCCCGGTGT 
AGAGACAAAG TCCCCAGGCC TCTGGAAGAG TGTGATGATG ATGAAGAAGG GATACATGCG 
GAAGACCTGA AAAGTGATCT AGAACTGCCT CCAGGATGGG AGAGAATAGA TGACCCAGTG 
TACGGAGTGT ATTATGTGGA CCATATAAAC AGGAAGACCC AGTATGAGAA CCCAGTTTTG 
GAGGCGCGGC ATCAAAAAAT CTTGGAGCAG CAGCAACCGC CGCAGCCTCC AGAAGGTGAG 
CGGTATATCC GAGAGTGGAT AGAGGAGCAT TCGTCTGCTG CAGCTCCATT GGCAAACTAT 
GTTCCAAACC ACCTTGAGAC ATACAGGGAC CCACAAGTCC CACCAGCTCT ACCTCCTGGC 
CCAGTCGGGG TTAAAAGGGG GAAGCCTTTC TTCACCAGAA ATCCTGCAGA ACTGAAAGGA 
ACCTTTATCA ACACCAAGCT AAAGAAAAGT CGCCGTGGAT TTGGCTTCAC AGTCGTTGGT 
GGCGATGAGC CTGATGAATT CCTGCAGATC AAAAGTCTGG TGCTGGACGG GCCTGCAGCT 
GTCGATGGCA AAATGGAGAC AGGGGATGTG ATTGTCAGTG TAAATGACAC CATCGTTCTG 
GGCTACACAC ATGCACAAGT GGTGAAGATT TTCCAGTCCA TCCCTATCGG CTCAATGGTG 
GACCTGGCCT TATGTCGAGG ATATCCGCTG CCATTTGACC CAGATGACCC AAACACCAGC 
CTAGTGACCT CTGTGGCGAT CTTGGACAAG GAGCCTATCA TTGTCAATGG ACAAGAGACT 
TTTGATGTAC CCGCCAACCA GGCTGGACCC ATCAATGGCA TGCGGGAGGC ACGGTCGCAC 
AGCCCCTCAG CGGAGGTGGC ATCAAACGGT TCACATGGGT ACTCCGCTGA CGTGGTCACC 
CTGGCCTCAG CCATCGCCAC CCAGCCAGAG CTGATCACCA TACACATGGA GAAGGGAGAC 
AAAGGGTTTG GCTTCACCAT TGCTGACAGC CTGACAGGAG GCGGGCAGAG AGTCAAGCAG 
ATAGTGGACT ACCCACGGTG CCGTGGCCTG AAAGAGGGGG ACATTCTGAT GGAGGTTAAC 
AAGAGAAACG TCCAGAATAT GAGCCACAAC CAGGTGGTTG ATCTGCTCAG CAAATGTCCG 
AGAGGCAGCG AAGTCACCAT GTTGGTGCAA AGAGGCGTGG TGCCAGCCAA GAGAAGCCCA 
AAGCTGGTGC ACCAGTTGGA GAGAAAGGAC AGTCAGACCA GCTCCCAGCA CAGCGTTTGC 
AGCCATCGAA GTGCTCACAC CGACTCTCCA AGCCACCCAC CCTCTGCCAT GCCAAGTGAG 
GCAGTGGTCC CCACTCCTGC TGCTCCCACA CAGCCTCTGC CGGTCTTGCC CCCCCAAGAC 
CCAGCAGATG GCACTCTCAC CCTCCAGAAG AAGCCAGACC CATTCAAGAT CTGGGCCCAG 
TCCAGAAGCA TGTATGAAAG TCGATTGCCA GATTGCCAGG AGCAAGACAT TTTCCTTTGG 
AGGAAGGATA CTGGCTTTGG CTTCCGCATT TTAGGAGGAA ATGAGCCTGG AGAACCTATT 
TACATTGGTC ATATAGTGAA ATATGGTGCT GCGGATGAAG ATGGCCGCCT GCGGTCTGGT 
GATGAGCTTA TTTGTGTGGA CGGCACAGCG GTAGTGGGCA AGTCCCACCA GCTGGTGGTT 
CAGCTGATGC AGCAGGCTGC CAAACAGGGT CATGTAAACC TCACCGTCAG GCGCAAGACC 
CCCGGATATG GAGTGTCAAA GGGAGAGGGT GATGTTCCGC CATCGCCGGC CTCTTCCCAT 
CACAGCAGCA CTCAGGCGCC AAGCCTCACA GAAGGCAAGC GGACCCCTCA GGGAAGCCAA 
AACTCCCTGA ATACTGTTAG CTCTGGCAGT GGATCTACCA GCGGTATAGG CAGTGGTGGT 
GGAGGTGGCA GTGGAAGCGC TGTGGTGCCC GCCTCTCTCC AGCCATACGA CGTAGAGATC 
CAGCGGGGAG AAAATGAGGG ATTTGGTTTC GTCATCGTAT CATCCGTCTC CCGGCCTGAT 
GCTGGAACCA CATTGGCTGG AAATGCATGC GTTGCGATGC CCCACAAAAT AGGTCGTATC 
ATAGAGGGCA GCCCGGCTGA CCGCTGCGGG AAGCTGAAGG TTGGAGACCG GATTCTGGCT 
GTCAATGGCT GCTCCATCAC AAACAAGTCA CATTCGGACA TCGTCAACCT GATCAAGGAG 
GCCGGGAACA CTGTCTCACT CAGGATCATT CCAGGCGATG AATCTTCCAA TGCTTCTCTC 
CTGACTAATG CAGAGAAAAT CGCTACCATC ACCACCACAC ACACACCTCA AACCACAGAG 
TTAAGGAATC ATTCAAAGTC AAAAGGAGTT CCTCCTCCAC CTCCTTCGCA AACTCATCTG 
CAGGATGCAG AATACTATTC TGTGGACCTT GAACGGGACA GTAAAGGCTT CGGCTTCAGC 
CTAAGAGGAG GACGGGAGTA CAACATGGAC CTTTATGTGC TGAGACTAGC CGAGGATGGG 
GCTGCTGTCA GAAATGGCAA AATGAGGGTG GGAGATGAGA TCCTGGAGAT TAATGGTGAG 
AGCACAAAGA ACATGAAGCA TTCACGAGCC ATTGAACTGA TCAAGAATGG AGGTCGGAGG 
GCAAGGCTTG TCCTGAAGAG AGGAGATGGA TCTGTACCCG AGTATGACGG CAGCAGTGAC 
GGGTACCCTT CCCCATCCGG GGCACAAAAC GCTCCGGAAG TGAGAAAGCT GCCGCCCAAC 
AGCCGATATC ACACCTCATT TGAGTCCAGT TATCCACCAG ACTTTCACAA ACCATCACGC 
CTGGAGGAGC CTTCGCGTGG CCGAAGTATG GAGAAGAACA GGGACTGGGC CGGCTCAGAT 
CTGATGGACT TCCATGGCCG GCAGTTTAAC CCCCACCTTC ATCACACCTG GAATAACAGT 
CACAGTCAAA GTATCCCCCG ACAGCAGTCT CCCGACAACA GTAATGGCAG CACCGTTGGC 
AACAAGAAGA GCCGAGGACG CAAAGTCAAG AAGTCTGAAT CAGAGAGTGG CATCTCCAAG 
CTCCTGAAAA GTCTAAGGAA AGACAGCTCA GGGAAGAAGG CTGCTGCGGC CGAAAAACTG 
CGAGGCAGAG AGCTCTCGAG CAGCAGTTCT ACTCTGGACA GTAAACTCCG TCCACAGCGT 
CGTGGCTCCC TTGATCGCTC ACCAACCAGG AAGCGTAACT CATCTCCGGA TCGTCGGCGG 
GCAAAGTCCA TGGACCGCAG AGCACATCGG GACCACACAC CTGAGAGGGA ATATGATGAC 
GGAGGGTTTC TCTCTGTAAC ACCTGAGAGA AAGCTCTATG AACGGAAGCT CCTCAAGGAG 
TCACCGTTAG CCGGTCGAGT CAGTGAGGTC TCCACCCCTG AGCGAGTCAA CAACAGTGCA 
GATTCTCTCA CCAGAGGCCG GGCATACGAT AAAGCCACTA AGAACGGAAA CCTTTTGAGA 
GACTCTTCCT CAGAAAGGAG GGAGGAGAGG CACAGAGGGA ATGGGACAAT GGAGAGGCGG 
TACAGACTGG AGCGGGACTC TTCCCCTGAC AGCAACTACA GTCGGGACGA GCTGAACTAT 
GCAGCAGCAG CAGCACCCAG ACTGAAGGAC TACTACAGCA TGATGAAGAA CAATGCTGCA 
CACAACAACG CTACCTACAA CAAGACAGGA CACAACAATA CTGCTGGAAG ACGCAGCTCA 
AACCAGATCC CTGAGCCCAA GAGAAGGCTG TACAAAGAAA ACCCAAAAGA CCTCAGTATC 
TAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107090390 ( XM_015383728.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015239214.1 Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X2, mRNA.

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CloneID	OCy14725
Clone ID Related Accession (Same CDS sequence)	XM_015383728.1
Accession Version	XM_015383728.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4860bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X2
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150746.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACAA AAGTAAATGA GTGCACGATT 
ATCCGGGACA CGAGTGGGGA GTTGAACGTG GAGCTGCACG GTGGAGCGGA GAATGGAGAG 
TTTACATACA TTGGAGATAT CAGGGAGGAT GCTGTGGTTT ATCAGGACGG AAAGCTGTCC 
AAGGGAGAAC TACTGCTAGA CGTGGAGGGT CTGTCGGTGT CTGGATTACC CCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAGGGA CCTGTCAGGT TTAAAACTGT TCGACAAGGC 
AACAAGCTCA ACAAGGACCT AAAGCACTTC TTGAGCTTAA GGTTTCAGAA AACATCACCT 
GACCACGAGT TGCAGAAAAC CATCCGAGCC AATCTATATC GCCATGCTGT GCCTTGTACG 
ACCCGCCCCC CTCGAGACGG AGAGGTCCCA GGGTTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTGGAGGA CAGTGGCACA CTACTGGAAG TAGGAACATA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC TCCAAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
GCTGGCTCCC TTCCGTCCAC ACCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAAAGTC 
CGCGTAGTGC CTGCTGACAA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGATCCT TAGTGCGTAG TCTCTTCCCC TCTCCTTTGC TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGCAG CGGCATCCTC CGGCCGTATC CCGTGCGTGA TAATGCTCTA 
CCCTGTGGTC TGAACAATGC AGCCATTTCC ACTGCAGAGA GCGGGCAGCA GACTCTTGCA 
GAGCCCCAGG TCTCACCAGA GGACCCACTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACAGAGA ACGGGGAACT GTACTTCATA GACACGACAA AGACTCTCCA CCAGGACATG 
GCTGTGTGTT TTCAGAGCTT CCACAACACA AAGACCACTT CATGGCTGGA TCCCCGGTGT 
AGAGACAAAG TCCCCAGGCC TCTGGAAGAG TGTGATGATG ATGAAGGGAT ACATGCGGAA 
GACCTGAAAA GTGATCTAGA ACTGCCTCCA GGATGGGAGA GAATAGATGA CCCAGTGTAC 
GGAGTGTATT ATGTGGACCA TATAAACAGG AAGACCCAGT ATGAGAACCC AGTTTTGGAG 
GCGCGGCATC AAAAAATCTT GGAGCAGCAG CAACCGCCGC AGCCTCCAGA AGGTGAGCGG 
TATATCCGAG AGTGGATAGA GGAGCATTCG TCTGCTGCAG CTCCATTGGC AAACTATGTT 
CCAAACCACC TTGAGACATA CAGGGACCCA CAAGTCCCAC CAGCTCTACC TCCTGGCCCA 
GTCGGGGTTA AAAGGGGGAA GCCTTTCTTC ACCAGAAATC CTGCAGAACT GAAAGGAACC 
TTTATCAACA CCAAGCTAAA GAAAAGTCGC CGTGGATTTG GCTTCACAGT CGTTGGTGGC 
GATGAGCCTG ATGAATTCCT GCAGATCAAA AGTCTGGTGC TGGACGGGCC TGCAGCTGTC 
GATGGCAAAA TGGAGACAGG GGATGTGATT GTCAGTGTAA ATGACACCAT CGTTCTGGGC 
TACACACATG CACAAGTGGT GAAGATTTTC CAGTCCATCC CTATCGGCTC AATGGTGGAC 
CTGGCCTTAT GTCGAGGATA TCCGCTGCCA TTTGACCCAG ATGACCCAAA CACCAGCCTA 
GTGACCTCTG TGGCGATCTT GGACAAGGAG CCTATCATTG TCAATGGACA AGAGACTTTT 
GATGTACCCG CCAACCAGGC TGGACCCATC AATGGCATGC GGGAGGCACG GTCGCACAGC 
CCCTCAGCGG AGGTGGCATC AAACGGTTCA CATGGGTACT CCGCTGACGT GGTCACCCTG 
GCCTCAGCCA TCGCCACCCA GCCAGAGCTG ATCACCATAC ACATGGAGAA GGGAGACAAA 
GGGTTTGGCT TCACCATTGC TGACAGCCTG ACAGGAGGCG GGCAGAGAGT CAAGCAGATA 
GTGGACTACC CACGGTGCCG TGGCCTGAAA GAGGGGGACA TTCTGATGGA GGTTAACAAG 
AGAAACGTCC AGAATATGAG CCACAACCAG GTGGTTGATC TGCTCAGCAA ATGTCCGAGA 
GGCAGCGAAG TCACCATGTT GGTGCAAAGA GGCGTGGTGC CAGCCAAGAG AAGCCCAAAG 
CTGGTGCACC AGTTGGAGAG AAAGGACAGT CAGACCAGCT CCCAGCACAG CGTTTGCAGC 
CATCGAAGTG CTCACACCGA CTCTCCAAGC CACCCACCCT CTGCCATGCC AAGTGAGGCA 
GTGGTCCCCA CTCCTGCTGC TCCCACACAG CCTCTGCCGG TCTTGCCCCC CCAAGACCCA 
GCAGATGGCA CTCTCACCCT CCAGAAGAAG CCAGACCCAT TCAAGATCTG GGCCCAGTCC 
AGAAGCATGT ATGAAAGTCG ATTGCCAGAT TGCCAGGAGC AAGACATTTT CCTTTGGAGG 
AAGGATACTG GCTTTGGCTT CCGCATTTTA GGAGGAAATG AGCCTGGAGA ACCTATTTAC 
ATTGGTCATA TAGTGAAATA TGGTGCTGCG GATGAAGATG GCCGCCTGCG GTCTGGTGAT 
GAGCTTATTT GTGTGGACGG CACAGCGGTA GTGGGCAAGT CCCACCAGCT GGTGGTTCAG 
CTGATGCAGC AGGCTGCCAA ACAGGGTCAT GTAAACCTCA CCGTCAGGCG CAAGACCCCC 
GGATATGGAG TGTCAAAGGG AGAGGGTGAT GTTCCGCCAT CGCCGGCCTC TTCCCATCAC 
AGCAGCACTC AGGCGCCAAG CCTCACAGAA GGCAAGCGGA CCCCTCAGGG AAGCCAAAAC 
TCCCTGAATA CTGTTAGCTC TGGCAGTGGA TCTACCAGCG GTATAGGCAG TGGTGGTGGA 
GGTGGCAGTG GAAGCGCTGT GGTGCCCGCC TCTCTCCAGC CATACGACGT AGAGATCCAG 
CGGGGAGAAA ATGAGGGATT TGGTTTCGTC ATCGTATCAT CCGTCTCCCG GCCTGATGCT 
GGAACCACAT TGGCTGGAAA TGCATGCGTT GCGATGCCCC ACAAAATAGG TCGTATCATA 
GAGGGCAGCC CGGCTGACCG CTGCGGGAAG CTGAAGGTTG GAGACCGGAT TCTGGCTGTC 
AATGGCTGCT CCATCACAAA CAAGTCACAT TCGGACATCG TCAACCTGAT CAAGGAGGCC 
GGGAACACTG TCTCACTCAG GATCATTCCA GGCGATGAAT CTTCCAATGC TTCTCTCCTG 
ACTAATGCAG AGAAAATCGC TACCATCACC ACCACACACA CACCTCAAAC CACAGAGTTA 
AGGAATCATT CAAAGTCAAA AGGAGTTCCT CCTCCACCTC CTTCGCAAAC TCATCTGCAG 
GATGCAGAAT ACTATTCTGT GGACCTTGAA CGGGACAGTA AAGGCTTCGG CTTCAGCCTA 
AGAGGAGGAC GGGAGTACAA CATGGACCTT TATGTGCTGA GACTAGCCGA GGATGGGGCT 
GCTGTCAGAA ATGGCAAAAT GAGGGTGGGA GATGAGATCC TGGAGATTAA TGGTGAGAGC 
ACAAAGAACA TGAAGCATTC ACGAGCCATT GAACTGATCA AGAATGGAGG TCGGAGGGCA 
AGGCTTGTCC TGAAGAGAGG AGATGGATCT GTACCCGAGT ATGACGGCAG CAGTGACGGG 
TACCCTTCCC CATCCGGGGC ACAAAACGCT CCGGAAGTGA GAAAGCTGCC GCCCAACAGC 
CGATATCACA CCTCATTTGA GTCCAGTTAT CCACCAGACT TTCACAAACC ATCACGCCTG 
GAGGAGCCTT CGCGTGGCCG AAGTATGGAG AAGAACAGGG ACTGGGCCGG CTCAGATCTG 
ATGGACTTCC ATGGCCGGCA GTTTAACCCC CACCTTCATC ACACCTGGAA TAACAGTCAC 
AGTCAAAGTA TCCCCCGACA GCAGTCTCCC GACAACAGTA ATGGCAGCAC CGTTGGCAAC 
AAGAAGAGCC GAGGACGCAA AGTCAAGAAG TCTGAATCAG AGAGTGGCAT CTCCAAGCTC 
CTGAAAAGTC TAAGGAAAGA CAGCTCAGGG AAGAAGGCTG CTGCGGCCGA AAAACTGCGA 
GGCAGAGAGC TCTCGAGCAG CAGTTCTACT CTGGACAGTA AACTCCGTCC ACAGCGTCGT 
GGCTCCCTTG ATCGCTCACC AACCAGGAAG CGTAACTCAT CTCCGGATCG TCGGCGGGCA 
AAGTCCATGG ACCGCAGAGC ACATCGGGAC CACACACCTG AGAGGGAATA TGATGACGGA 
GGGTTTCTCT CTGTAACACC TGAGAGAAAG CTCTATGAAC GGAAGCTCCT CAAGGAGTCA 
CCGTTAGCCG GTCGAGTCAG TGAGGTCTCC ACCCCTGAGC GAGTCAACAA CAGTGCAGAT 
TCTCTCACCA GAGGCCGGGC ATACGATAAA GCCACTAAGA ACGGAAACCT TTTGAGAGAC 
TCTTCCTCAG AAAGGAGGGA GGAGAGGCAC AGAGGGAATG GGACAATGGA GAGGCGGTAC 
AGACTGGAGC GGGACTCTTC CCCTGACAGC AACTACAGTC GGGACGAGCT GAACTATGCA 
GCAGCAGCAG CACCCAGACT GAAGGACTAC TACAGCATGA TGAAGAACAA TGCTGCACAC 
AACAACGCTA CCTACAACAA GACAGGACAC AACAATACTG CTGGAAGACG CAGCTCAAAC 
CAGATCCCTG AGCCCAAGAG AAGGCTGTAC AAAGAAAACC CAAAAGACCT CAGTATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015239214.1
CDS	575..5434
Translation	MAKVIQKKNHWITKVNECTIIRDTSGELNVELHGGAENGEFTYIGDIREDAVVYQDGKLSKGELLLDVEGLSVSGLPLYDIYTVINCCKGPVRFKTVRQGNKLNKDLKHFLSLRFQKTSPDHELQKTIRANLYRHAVPCTTRPPRDGEVPGLDYNFLSVEDFLQLEDSGTLLEVGTYEGNYYGTPKPPRQPIIGTVTLSDAGSLPSTPKRTKSYNDMQKVRVVPADNDDDDQATEMNNSFTGSLVRSLFPSPLLCTGNPNDQDESRSGILRPYPVRDNALPCGLNNAAISTAESGQQTLAEPQVSPEDPLGPLPENWEMAYTENGELYFIDTTKTLHQDMAVCFQSFHNTKTTSWLDPRCRDKVPRPLEECDDDEGIHAEDLKSDLELPPGWERIDDPVYGVYYVDHINRKTQYENPVLEARHQKILEQQQPPQPPEGERYIREWIEEHSSAAAPLANYVPNHLETYRDPQVPPALPPGPVGVKRGKPFFTRNPAELKGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAAVDGKMETGDVIVSVNDTIVLGYTHAQVVKIFQSIPIGSMVDLALCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETFDVPANQAGPINGMREARSHSPSAEVASNGSHGYSADVVTLASAIATQPELITIHMEKGDKGFGFTIADSLTGGGQRVKQIVDYPRCRGLKEGDILMEVNKRNVQNMSHNQVVDLLSKCPRGSEVTMLVQRGVVPAKRSPKLVHQLERKDSQTSSQHSVCSHRSAHTDSPSHPPSAMPSEAVVPTPAAPTQPLPVLPPQDPADGTLTLQKKPDPFKIWAQSRSMYESRLPDCQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTPGYGVSKGEGDVPPSPASSHHSSTQAPSLTEGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGSAVVPASLQPYDVEIQRGENEGFGFVIVSSVSRPDAGTTLAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVSLRIIPGDESSNASLLTNAEKIATITTTHTPQTTELRNHSKSKGVPPPPPSQTHLQDAEYYSVDLERDSKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKNMKHSRAIELIKNGGRRARLVLKRGDGSVPEYDGSSDGYPSPSGAQNAPEVRKLPPNSRYHTSFESSYPPDFHKPSRLEEPSRGRSMEKNRDWAGSDLMDFHGRQFNPHLHHTWNNSHSQSIPRQQSPDNSNGSTVGNKKSRGRKVKKSESESGISKLLKSLRKDSSGKKAAAAEKLRGRELSSSSSTLDSKLRPQRRGSLDRSPTRKRNSSPDRRRAKSMDRRAHRDHTPEREYDDGGFLSVTPERKLYERKLLKESPLAGRVSEVSTPERVNNSADSLTRGRAYDKATKNGNLLRDSSSERREERHRGNGTMERRYRLERDSSPDSNYSRDELNYAAAAAPRLKDYYSMMKNNAAHNNATYNKTGHNNTAGRRSSNQIPEPKRRLYKENPKDLSI

Target ORF information:

RefSeq Version	XM_015383728.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X2, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015383728.1

ORF Insert Sequence:

ATGGCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACAA AAGTAAATGA GTGCACGATT 
ATCCGGGACA CGAGTGGGGA GTTGAACGTG GAGCTGCACG GTGGAGCGGA GAATGGAGAG 
TTTACATACA TTGGAGATAT CAGGGAGGAT GCTGTGGTTT ATCAGGACGG AAAGCTGTCC 
AAGGGAGAAC TACTGCTAGA CGTGGAGGGT CTGTCGGTGT CTGGATTACC CCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAGGGA CCTGTCAGGT TTAAAACTGT TCGACAAGGC 
AACAAGCTCA ACAAGGACCT AAAGCACTTC TTGAGCTTAA GGTTTCAGAA AACATCACCT 
GACCACGAGT TGCAGAAAAC CATCCGAGCC AATCTATATC GCCATGCTGT GCCTTGTACG 
ACCCGCCCCC CTCGAGACGG AGAGGTCCCA GGGTTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTGGAGGA CAGTGGCACA CTACTGGAAG TAGGAACATA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC TCCAAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
GCTGGCTCCC TTCCGTCCAC ACCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAAAGTC 
CGCGTAGTGC CTGCTGACAA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGATCCT TAGTGCGTAG TCTCTTCCCC TCTCCTTTGC TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGCAG CGGCATCCTC CGGCCGTATC CCGTGCGTGA TAATGCTCTA 
CCCTGTGGTC TGAACAATGC AGCCATTTCC ACTGCAGAGA GCGGGCAGCA GACTCTTGCA 
GAGCCCCAGG TCTCACCAGA GGACCCACTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACAGAGA ACGGGGAACT GTACTTCATA GACACGACAA AGACTCTCCA CCAGGACATG 
GCTGTGTGTT TTCAGAGCTT CCACAACACA AAGACCACTT CATGGCTGGA TCCCCGGTGT 
AGAGACAAAG TCCCCAGGCC TCTGGAAGAG TGTGATGATG ATGAAGGGAT ACATGCGGAA 
GACCTGAAAA GTGATCTAGA ACTGCCTCCA GGATGGGAGA GAATAGATGA CCCAGTGTAC 
GGAGTGTATT ATGTGGACCA TATAAACAGG AAGACCCAGT ATGAGAACCC AGTTTTGGAG 
GCGCGGCATC AAAAAATCTT GGAGCAGCAG CAACCGCCGC AGCCTCCAGA AGGTGAGCGG 
TATATCCGAG AGTGGATAGA GGAGCATTCG TCTGCTGCAG CTCCATTGGC AAACTATGTT 
CCAAACCACC TTGAGACATA CAGGGACCCA CAAGTCCCAC CAGCTCTACC TCCTGGCCCA 
GTCGGGGTTA AAAGGGGGAA GCCTTTCTTC ACCAGAAATC CTGCAGAACT GAAAGGAACC 
TTTATCAACA CCAAGCTAAA GAAAAGTCGC CGTGGATTTG GCTTCACAGT CGTTGGTGGC 
GATGAGCCTG ATGAATTCCT GCAGATCAAA AGTCTGGTGC TGGACGGGCC TGCAGCTGTC 
GATGGCAAAA TGGAGACAGG GGATGTGATT GTCAGTGTAA ATGACACCAT CGTTCTGGGC 
TACACACATG CACAAGTGGT GAAGATTTTC CAGTCCATCC CTATCGGCTC AATGGTGGAC 
CTGGCCTTAT GTCGAGGATA TCCGCTGCCA TTTGACCCAG ATGACCCAAA CACCAGCCTA 
GTGACCTCTG TGGCGATCTT GGACAAGGAG CCTATCATTG TCAATGGACA AGAGACTTTT 
GATGTACCCG CCAACCAGGC TGGACCCATC AATGGCATGC GGGAGGCACG GTCGCACAGC 
CCCTCAGCGG AGGTGGCATC AAACGGTTCA CATGGGTACT CCGCTGACGT GGTCACCCTG 
GCCTCAGCCA TCGCCACCCA GCCAGAGCTG ATCACCATAC ACATGGAGAA GGGAGACAAA 
GGGTTTGGCT TCACCATTGC TGACAGCCTG ACAGGAGGCG GGCAGAGAGT CAAGCAGATA 
GTGGACTACC CACGGTGCCG TGGCCTGAAA GAGGGGGACA TTCTGATGGA GGTTAACAAG 
AGAAACGTCC AGAATATGAG CCACAACCAG GTGGTTGATC TGCTCAGCAA ATGTCCGAGA 
GGCAGCGAAG TCACCATGTT GGTGCAAAGA GGCGTGGTGC CAGCCAAGAG AAGCCCAAAG 
CTGGTGCACC AGTTGGAGAG AAAGGACAGT CAGACCAGCT CCCAGCACAG CGTTTGCAGC 
CATCGAAGTG CTCACACCGA CTCTCCAAGC CACCCACCCT CTGCCATGCC AAGTGAGGCA 
GTGGTCCCCA CTCCTGCTGC TCCCACACAG CCTCTGCCGG TCTTGCCCCC CCAAGACCCA 
GCAGATGGCA CTCTCACCCT CCAGAAGAAG CCAGACCCAT TCAAGATCTG GGCCCAGTCC 
AGAAGCATGT ATGAAAGTCG ATTGCCAGAT TGCCAGGAGC AAGACATTTT CCTTTGGAGG 
AAGGATACTG GCTTTGGCTT CCGCATTTTA GGAGGAAATG AGCCTGGAGA ACCTATTTAC 
ATTGGTCATA TAGTGAAATA TGGTGCTGCG GATGAAGATG GCCGCCTGCG GTCTGGTGAT 
GAGCTTATTT GTGTGGACGG CACAGCGGTA GTGGGCAAGT CCCACCAGCT GGTGGTTCAG 
CTGATGCAGC AGGCTGCCAA ACAGGGTCAT GTAAACCTCA CCGTCAGGCG CAAGACCCCC 
GGATATGGAG TGTCAAAGGG AGAGGGTGAT GTTCCGCCAT CGCCGGCCTC TTCCCATCAC 
AGCAGCACTC AGGCGCCAAG CCTCACAGAA GGCAAGCGGA CCCCTCAGGG AAGCCAAAAC 
TCCCTGAATA CTGTTAGCTC TGGCAGTGGA TCTACCAGCG GTATAGGCAG TGGTGGTGGA 
GGTGGCAGTG GAAGCGCTGT GGTGCCCGCC TCTCTCCAGC CATACGACGT AGAGATCCAG 
CGGGGAGAAA ATGAGGGATT TGGTTTCGTC ATCGTATCAT CCGTCTCCCG GCCTGATGCT 
GGAACCACAT TGGCTGGAAA TGCATGCGTT GCGATGCCCC ACAAAATAGG TCGTATCATA 
GAGGGCAGCC CGGCTGACCG CTGCGGGAAG CTGAAGGTTG GAGACCGGAT TCTGGCTGTC 
AATGGCTGCT CCATCACAAA CAAGTCACAT TCGGACATCG TCAACCTGAT CAAGGAGGCC 
GGGAACACTG TCTCACTCAG GATCATTCCA GGCGATGAAT CTTCCAATGC TTCTCTCCTG 
ACTAATGCAG AGAAAATCGC TACCATCACC ACCACACACA CACCTCAAAC CACAGAGTTA 
AGGAATCATT CAAAGTCAAA AGGAGTTCCT CCTCCACCTC CTTCGCAAAC TCATCTGCAG 
GATGCAGAAT ACTATTCTGT GGACCTTGAA CGGGACAGTA AAGGCTTCGG CTTCAGCCTA 
AGAGGAGGAC GGGAGTACAA CATGGACCTT TATGTGCTGA GACTAGCCGA GGATGGGGCT 
GCTGTCAGAA ATGGCAAAAT GAGGGTGGGA GATGAGATCC TGGAGATTAA TGGTGAGAGC 
ACAAAGAACA TGAAGCATTC ACGAGCCATT GAACTGATCA AGAATGGAGG TCGGAGGGCA 
AGGCTTGTCC TGAAGAGAGG AGATGGATCT GTACCCGAGT ATGACGGCAG CAGTGACGGG 
TACCCTTCCC CATCCGGGGC ACAAAACGCT CCGGAAGTGA GAAAGCTGCC GCCCAACAGC 
CGATATCACA CCTCATTTGA GTCCAGTTAT CCACCAGACT TTCACAAACC ATCACGCCTG 
GAGGAGCCTT CGCGTGGCCG AAGTATGGAG AAGAACAGGG ACTGGGCCGG CTCAGATCTG 
ATGGACTTCC ATGGCCGGCA GTTTAACCCC CACCTTCATC ACACCTGGAA TAACAGTCAC 
AGTCAAAGTA TCCCCCGACA GCAGTCTCCC GACAACAGTA ATGGCAGCAC CGTTGGCAAC 
AAGAAGAGCC GAGGACGCAA AGTCAAGAAG TCTGAATCAG AGAGTGGCAT CTCCAAGCTC 
CTGAAAAGTC TAAGGAAAGA CAGCTCAGGG AAGAAGGCTG CTGCGGCCGA AAAACTGCGA 
GGCAGAGAGC TCTCGAGCAG CAGTTCTACT CTGGACAGTA AACTCCGTCC ACAGCGTCGT 
GGCTCCCTTG ATCGCTCACC AACCAGGAAG CGTAACTCAT CTCCGGATCG TCGGCGGGCA 
AAGTCCATGG ACCGCAGAGC ACATCGGGAC CACACACCTG AGAGGGAATA TGATGACGGA 
GGGTTTCTCT CTGTAACACC TGAGAGAAAG CTCTATGAAC GGAAGCTCCT CAAGGAGTCA 
CCGTTAGCCG GTCGAGTCAG TGAGGTCTCC ACCCCTGAGC GAGTCAACAA CAGTGCAGAT 
TCTCTCACCA GAGGCCGGGC ATACGATAAA GCCACTAAGA ACGGAAACCT TTTGAGAGAC 
TCTTCCTCAG AAAGGAGGGA GGAGAGGCAC AGAGGGAATG GGACAATGGA GAGGCGGTAC 
AGACTGGAGC GGGACTCTTC CCCTGACAGC AACTACAGTC GGGACGAGCT GAACTATGCA 
GCAGCAGCAG CACCCAGACT GAAGGACTAC TACAGCATGA TGAAGAACAA TGCTGCACAC 
AACAACGCTA CCTACAACAA GACAGGACAC AACAATACTG CTGGAAGACG CAGCTCAAAC 
CAGATCCCTG AGCCCAAGAG AAGGCTGTAC AAAGAAAACC CAAAAGACCT CAGTATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107090390 ( XM_015383729.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015239215.1 Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X3, mRNA.

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CloneID	OCy14726
Clone ID Related Accession (Same CDS sequence)	XM_015383729.1
Accession Version	XM_015383729.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4845bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X3
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150746.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACAA AAGTAAATGA GTGCACGATT 
ATCCGGGACA CGAGTGGGGA GTTGAACGTG GAGCTGCACG GTGGAGCGGA GAATGGAGAG 
TTTACATACA TTGGAGATAT CAGGGAGGAT GCTGTGGTTT ATCAGGACGG AAAGCTGTCC 
AAGGGAGAAC TACTGCTAGA CGTGGAGGGT CTGTCGGTGT CTGGATTACC CCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAGGGA CCTGTCAGGT TTAAAACTGT TCGACAAGGC 
AACAAGCTCA ACAAGGACCT AAAGCACTTC TTGAGCTTAA GGTTTCAGAA AACATCACCT 
GACCACGAGT TGCAGAAAAC CATCCGAGCC AATCTATATC GCCATGCTGT GCCTTGTACG 
ACCCGCCCCC CTCGAGACGG AGAGGTCCCA GGGTTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTGGAGGA CAGTGGCACA CTACTGGAAG TAGGAACATA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC TCCAAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
GCTGGCTCCC TTCCGTCCAC ACCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAAAGTC 
CGCGTAGTGC CTGCTGACAA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGATCCT TAGTGCGTAG TCTCTTCCCC TCTCCTTTGC TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGCAG CGGCATCCTC CGGCCGTATC CCGTGCGTGA TAATGCTCTA 
CCCTGTGGTC TGAACAATGC AGCCATTTCC ACTGCAGAGA GCGGGCAGCA GACTCTTGCA 
GAGCCCCAGG TCTCACCAGA GGACCCACTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACAGAGA ACGGGGAACT GTACTTCATA GACACGACAA AGACTCTCCA CCAGGACATG 
GCTGTGTGTT TTCAGAGCTT CCACAACACA AAGACCACTT CATGGCTGGA TCCCCGGTGT 
AGAGACAAAG TCCCCAGGCC TCTGGAAGAG TGTGATGATG ATGAAGAAGG GATACATGCG 
GAAGACCTGA AAAGTGATCT AGAACTGCCT CCAGGATGGG AGAGAATAGA TGACCCAGTG 
TACGGAGTGT ATTATGTGGA CCATATAAAC AGGAAGACCC AGTATGAGAA CCCAGTTTTG 
GAGGCGCGGC ATCAAAAAAT CTTGGAGCAG CAGCAACCGC CGCAGCCTCC AGAAGAGTGG 
ATAGAGGAGC ATTCGTCTGC TGCAGCTCCA TTGGCAAACT ATGTTCCAAA CCACCTTGAG 
ACATACAGGG ACCCACAAGT CCCACCAGCT CTACCTCCTG GCCCAGTCGG GGTTAAAAGG 
GGGAAGCCTT TCTTCACCAG AAATCCTGCA GAACTGAAAG GAACCTTTAT CAACACCAAG 
CTAAAGAAAA GTCGCCGTGG ATTTGGCTTC ACAGTCGTTG GTGGCGATGA GCCTGATGAA 
TTCCTGCAGA TCAAAAGTCT GGTGCTGGAC GGGCCTGCAG CTGTCGATGG CAAAATGGAG 
ACAGGGGATG TGATTGTCAG TGTAAATGAC ACCATCGTTC TGGGCTACAC ACATGCACAA 
GTGGTGAAGA TTTTCCAGTC CATCCCTATC GGCTCAATGG TGGACCTGGC CTTATGTCGA 
GGATATCCGC TGCCATTTGA CCCAGATGAC CCAAACACCA GCCTAGTGAC CTCTGTGGCG 
ATCTTGGACA AGGAGCCTAT CATTGTCAAT GGACAAGAGA CTTTTGATGT ACCCGCCAAC 
CAGGCTGGAC CCATCAATGG CATGCGGGAG GCACGGTCGC ACAGCCCCTC AGCGGAGGTG 
GCATCAAACG GTTCACATGG GTACTCCGCT GACGTGGTCA CCCTGGCCTC AGCCATCGCC 
ACCCAGCCAG AGCTGATCAC CATACACATG GAGAAGGGAG ACAAAGGGTT TGGCTTCACC 
ATTGCTGACA GCCTGACAGG AGGCGGGCAG AGAGTCAAGC AGATAGTGGA CTACCCACGG 
TGCCGTGGCC TGAAAGAGGG GGACATTCTG ATGGAGGTTA ACAAGAGAAA CGTCCAGAAT 
ATGAGCCACA ACCAGGTGGT TGATCTGCTC AGCAAATGTC CGAGAGGCAG CGAAGTCACC 
ATGTTGGTGC AAAGAGGCGT GGTGCCAGCC AAGAGAAGCC CAAAGCTGGT GCACCAGTTG 
GAGAGAAAGG ACAGTCAGAC CAGCTCCCAG CACAGCGTTT GCAGCCATCG AAGTGCTCAC 
ACCGACTCTC CAAGCCACCC ACCCTCTGCC ATGCCAAGTG AGGCAGTGGT CCCCACTCCT 
GCTGCTCCCA CACAGCCTCT GCCGGTCTTG CCCCCCCAAG ACCCAGCAGA TGGCACTCTC 
ACCCTCCAGA AGAAGCCAGA CCCATTCAAG ATCTGGGCCC AGTCCAGAAG CATGTATGAA 
AGTCGATTGC CAGATTGCCA GGAGCAAGAC ATTTTCCTTT GGAGGAAGGA TACTGGCTTT 
GGCTTCCGCA TTTTAGGAGG AAATGAGCCT GGAGAACCTA TTTACATTGG TCATATAGTG 
AAATATGGTG CTGCGGATGA AGATGGCCGC CTGCGGTCTG GTGATGAGCT TATTTGTGTG 
GACGGCACAG CGGTAGTGGG CAAGTCCCAC CAGCTGGTGG TTCAGCTGAT GCAGCAGGCT 
GCCAAACAGG GTCATGTAAA CCTCACCGTC AGGCGCAAGA CCCCCGGATA TGGAGTGTCA 
AAGGGAGAGG GTGATGTTCC GCCATCGCCG GCCTCTTCCC ATCACAGCAG CACTCAGGCG 
CCAAGCCTCA CAGAAGGCAA GCGGACCCCT CAGGGAAGCC AAAACTCCCT GAATACTGTT 
AGCTCTGGCA GTGGATCTAC CAGCGGTATA GGCAGTGGTG GTGGAGGTGG CAGTGGAAGC 
GCTGTGGTGC CCGCCTCTCT CCAGCCATAC GACGTAGAGA TCCAGCGGGG AGAAAATGAG 
GGATTTGGTT TCGTCATCGT ATCATCCGTC TCCCGGCCTG ATGCTGGAAC CACATTGGCT 
GGAAATGCAT GCGTTGCGAT GCCCCACAAA ATAGGTCGTA TCATAGAGGG CAGCCCGGCT 
GACCGCTGCG GGAAGCTGAA GGTTGGAGAC CGGATTCTGG CTGTCAATGG CTGCTCCATC 
ACAAACAAGT CACATTCGGA CATCGTCAAC CTGATCAAGG AGGCCGGGAA CACTGTCTCA 
CTCAGGATCA TTCCAGGCGA TGAATCTTCC AATGCTTCTC TCCTGACTAA TGCAGAGAAA 
ATCGCTACCA TCACCACCAC ACACACACCT CAAACCACAG AGTTAAGGAA TCATTCAAAG 
TCAAAAGGAG TTCCTCCTCC ACCTCCTTCG CAAACTCATC TGCAGGATGC AGAATACTAT 
TCTGTGGACC TTGAACGGGA CAGTAAAGGC TTCGGCTTCA GCCTAAGAGG AGGACGGGAG 
TACAACATGG ACCTTTATGT GCTGAGACTA GCCGAGGATG GGGCTGCTGT CAGAAATGGC 
AAAATGAGGG TGGGAGATGA GATCCTGGAG ATTAATGGTG AGAGCACAAA GAACATGAAG 
CATTCACGAG CCATTGAACT GATCAAGAAT GGAGGTCGGA GGGCAAGGCT TGTCCTGAAG 
AGAGGAGATG GATCTGTACC CGAGTATGAC GGCAGCAGTG ACGGGTACCC TTCCCCATCC 
GGGGCACAAA ACGCTCCGGA AGTGAGAAAG CTGCCGCCCA ACAGCCGATA TCACACCTCA 
TTTGAGTCCA GTTATCCACC AGACTTTCAC AAACCATCAC GCCTGGAGGA GCCTTCGCGT 
GGCCGAAGTA TGGAGAAGAA CAGGGACTGG GCCGGCTCAG ATCTGATGGA CTTCCATGGC 
CGGCAGTTTA ACCCCCACCT TCATCACACC TGGAATAACA GTCACAGTCA AAGTATCCCC 
CGACAGCAGT CTCCCGACAA CAGTAATGGC AGCACCGTTG GCAACAAGAA GAGCCGAGGA 
CGCAAAGTCA AGAAGTCTGA ATCAGAGAGT GGCATCTCCA AGCTCCTGAA AAGTCTAAGG 
AAAGACAGCT CAGGGAAGAA GGCTGCTGCG GCCGAAAAAC TGCGAGGCAG AGAGCTCTCG 
AGCAGCAGTT CTACTCTGGA CAGTAAACTC CGTCCACAGC GTCGTGGCTC CCTTGATCGC 
TCACCAACCA GGAAGCGTAA CTCATCTCCG GATCGTCGGC GGGCAAAGTC CATGGACCGC 
AGAGCACATC GGGACCACAC ACCTGAGAGG GAATATGATG ACGGAGGGTT TCTCTCTGTA 
ACACCTGAGA GAAAGCTCTA TGAACGGAAG CTCCTCAAGG AGTCACCGTT AGCCGGTCGA 
GTCAGTGAGG TCTCCACCCC TGAGCGAGTC AACAACAGTG CAGATTCTCT CACCAGAGGC 
CGGGCATACG ATAAAGCCAC TAAGAACGGA AACCTTTTGA GAGACTCTTC CTCAGAAAGG 
AGGGAGGAGA GGCACAGAGG GAATGGGACA ATGGAGAGGC GGTACAGACT GGAGCGGGAC 
TCTTCCCCTG ACAGCAACTA CAGTCGGGAC GAGCTGAACT ATGCAGCAGC AGCAGCACCC 
AGACTGAAGG ACTACTACAG CATGATGAAG AACAATGCTG CACACAACAA CGCTACCTAC 
AACAAGACAG GACACAACAA TACTGCTGGA AGACGCAGCT CAAACCAGAT CCCTGAGCCC 
AAGAGAAGGC TGTACAAAGA AAACCCAAAA GACCTCAGTA TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015239215.1
CDS	575..5419
Translation	MAKVIQKKNHWITKVNECTIIRDTSGELNVELHGGAENGEFTYIGDIREDAVVYQDGKLSKGELLLDVEGLSVSGLPLYDIYTVINCCKGPVRFKTVRQGNKLNKDLKHFLSLRFQKTSPDHELQKTIRANLYRHAVPCTTRPPRDGEVPGLDYNFLSVEDFLQLEDSGTLLEVGTYEGNYYGTPKPPRQPIIGTVTLSDAGSLPSTPKRTKSYNDMQKVRVVPADNDDDDQATEMNNSFTGSLVRSLFPSPLLCTGNPNDQDESRSGILRPYPVRDNALPCGLNNAAISTAESGQQTLAEPQVSPEDPLGPLPENWEMAYTENGELYFIDTTKTLHQDMAVCFQSFHNTKTTSWLDPRCRDKVPRPLEECDDDEEGIHAEDLKSDLELPPGWERIDDPVYGVYYVDHINRKTQYENPVLEARHQKILEQQQPPQPPEEWIEEHSSAAAPLANYVPNHLETYRDPQVPPALPPGPVGVKRGKPFFTRNPAELKGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAAVDGKMETGDVIVSVNDTIVLGYTHAQVVKIFQSIPIGSMVDLALCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETFDVPANQAGPINGMREARSHSPSAEVASNGSHGYSADVVTLASAIATQPELITIHMEKGDKGFGFTIADSLTGGGQRVKQIVDYPRCRGLKEGDILMEVNKRNVQNMSHNQVVDLLSKCPRGSEVTMLVQRGVVPAKRSPKLVHQLERKDSQTSSQHSVCSHRSAHTDSPSHPPSAMPSEAVVPTPAAPTQPLPVLPPQDPADGTLTLQKKPDPFKIWAQSRSMYESRLPDCQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTPGYGVSKGEGDVPPSPASSHHSSTQAPSLTEGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGSAVVPASLQPYDVEIQRGENEGFGFVIVSSVSRPDAGTTLAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVSLRIIPGDESSNASLLTNAEKIATITTTHTPQTTELRNHSKSKGVPPPPPSQTHLQDAEYYSVDLERDSKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKNMKHSRAIELIKNGGRRARLVLKRGDGSVPEYDGSSDGYPSPSGAQNAPEVRKLPPNSRYHTSFESSYPPDFHKPSRLEEPSRGRSMEKNRDWAGSDLMDFHGRQFNPHLHHTWNNSHSQSIPRQQSPDNSNGSTVGNKKSRGRKVKKSESESGISKLLKSLRKDSSGKKAAAAEKLRGRELSSSSSTLDSKLRPQRRGSLDRSPTRKRNSSPDRRRAKSMDRRAHRDHTPEREYDDGGFLSVTPERKLYERKLLKESPLAGRVSEVSTPERVNNSADSLTRGRAYDKATKNGNLLRDSSSERREERHRGNGTMERRYRLERDSSPDSNYSRDELNYAAAAAPRLKDYYSMMKNNAAHNNATYNKTGHNNTAGRRSSNQIPEPKRRLYKENPKDLSI

Target ORF information:

RefSeq Version	XM_015383729.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X3, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015383729.1

ORF Insert Sequence:

ATGGCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACAA AAGTAAATGA GTGCACGATT 
ATCCGGGACA CGAGTGGGGA GTTGAACGTG GAGCTGCACG GTGGAGCGGA GAATGGAGAG 
TTTACATACA TTGGAGATAT CAGGGAGGAT GCTGTGGTTT ATCAGGACGG AAAGCTGTCC 
AAGGGAGAAC TACTGCTAGA CGTGGAGGGT CTGTCGGTGT CTGGATTACC CCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAGGGA CCTGTCAGGT TTAAAACTGT TCGACAAGGC 
AACAAGCTCA ACAAGGACCT AAAGCACTTC TTGAGCTTAA GGTTTCAGAA AACATCACCT 
GACCACGAGT TGCAGAAAAC CATCCGAGCC AATCTATATC GCCATGCTGT GCCTTGTACG 
ACCCGCCCCC CTCGAGACGG AGAGGTCCCA GGGTTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTGGAGGA CAGTGGCACA CTACTGGAAG TAGGAACATA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC TCCAAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
GCTGGCTCCC TTCCGTCCAC ACCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAAAGTC 
CGCGTAGTGC CTGCTGACAA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGATCCT TAGTGCGTAG TCTCTTCCCC TCTCCTTTGC TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGCAG CGGCATCCTC CGGCCGTATC CCGTGCGTGA TAATGCTCTA 
CCCTGTGGTC TGAACAATGC AGCCATTTCC ACTGCAGAGA GCGGGCAGCA GACTCTTGCA 
GAGCCCCAGG TCTCACCAGA GGACCCACTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACAGAGA ACGGGGAACT GTACTTCATA GACACGACAA AGACTCTCCA CCAGGACATG 
GCTGTGTGTT TTCAGAGCTT CCACAACACA AAGACCACTT CATGGCTGGA TCCCCGGTGT 
AGAGACAAAG TCCCCAGGCC TCTGGAAGAG TGTGATGATG ATGAAGAAGG GATACATGCG 
GAAGACCTGA AAAGTGATCT AGAACTGCCT CCAGGATGGG AGAGAATAGA TGACCCAGTG 
TACGGAGTGT ATTATGTGGA CCATATAAAC AGGAAGACCC AGTATGAGAA CCCAGTTTTG 
GAGGCGCGGC ATCAAAAAAT CTTGGAGCAG CAGCAACCGC CGCAGCCTCC AGAAGAGTGG 
ATAGAGGAGC ATTCGTCTGC TGCAGCTCCA TTGGCAAACT ATGTTCCAAA CCACCTTGAG 
ACATACAGGG ACCCACAAGT CCCACCAGCT CTACCTCCTG GCCCAGTCGG GGTTAAAAGG 
GGGAAGCCTT TCTTCACCAG AAATCCTGCA GAACTGAAAG GAACCTTTAT CAACACCAAG 
CTAAAGAAAA GTCGCCGTGG ATTTGGCTTC ACAGTCGTTG GTGGCGATGA GCCTGATGAA 
TTCCTGCAGA TCAAAAGTCT GGTGCTGGAC GGGCCTGCAG CTGTCGATGG CAAAATGGAG 
ACAGGGGATG TGATTGTCAG TGTAAATGAC ACCATCGTTC TGGGCTACAC ACATGCACAA 
GTGGTGAAGA TTTTCCAGTC CATCCCTATC GGCTCAATGG TGGACCTGGC CTTATGTCGA 
GGATATCCGC TGCCATTTGA CCCAGATGAC CCAAACACCA GCCTAGTGAC CTCTGTGGCG 
ATCTTGGACA AGGAGCCTAT CATTGTCAAT GGACAAGAGA CTTTTGATGT ACCCGCCAAC 
CAGGCTGGAC CCATCAATGG CATGCGGGAG GCACGGTCGC ACAGCCCCTC AGCGGAGGTG 
GCATCAAACG GTTCACATGG GTACTCCGCT GACGTGGTCA CCCTGGCCTC AGCCATCGCC 
ACCCAGCCAG AGCTGATCAC CATACACATG GAGAAGGGAG ACAAAGGGTT TGGCTTCACC 
ATTGCTGACA GCCTGACAGG AGGCGGGCAG AGAGTCAAGC AGATAGTGGA CTACCCACGG 
TGCCGTGGCC TGAAAGAGGG GGACATTCTG ATGGAGGTTA ACAAGAGAAA CGTCCAGAAT 
ATGAGCCACA ACCAGGTGGT TGATCTGCTC AGCAAATGTC CGAGAGGCAG CGAAGTCACC 
ATGTTGGTGC AAAGAGGCGT GGTGCCAGCC AAGAGAAGCC CAAAGCTGGT GCACCAGTTG 
GAGAGAAAGG ACAGTCAGAC CAGCTCCCAG CACAGCGTTT GCAGCCATCG AAGTGCTCAC 
ACCGACTCTC CAAGCCACCC ACCCTCTGCC ATGCCAAGTG AGGCAGTGGT CCCCACTCCT 
GCTGCTCCCA CACAGCCTCT GCCGGTCTTG CCCCCCCAAG ACCCAGCAGA TGGCACTCTC 
ACCCTCCAGA AGAAGCCAGA CCCATTCAAG ATCTGGGCCC AGTCCAGAAG CATGTATGAA 
AGTCGATTGC CAGATTGCCA GGAGCAAGAC ATTTTCCTTT GGAGGAAGGA TACTGGCTTT 
GGCTTCCGCA TTTTAGGAGG AAATGAGCCT GGAGAACCTA TTTACATTGG TCATATAGTG 
AAATATGGTG CTGCGGATGA AGATGGCCGC CTGCGGTCTG GTGATGAGCT TATTTGTGTG 
GACGGCACAG CGGTAGTGGG CAAGTCCCAC CAGCTGGTGG TTCAGCTGAT GCAGCAGGCT 
GCCAAACAGG GTCATGTAAA CCTCACCGTC AGGCGCAAGA CCCCCGGATA TGGAGTGTCA 
AAGGGAGAGG GTGATGTTCC GCCATCGCCG GCCTCTTCCC ATCACAGCAG CACTCAGGCG 
CCAAGCCTCA CAGAAGGCAA GCGGACCCCT CAGGGAAGCC AAAACTCCCT GAATACTGTT 
AGCTCTGGCA GTGGATCTAC CAGCGGTATA GGCAGTGGTG GTGGAGGTGG CAGTGGAAGC 
GCTGTGGTGC CCGCCTCTCT CCAGCCATAC GACGTAGAGA TCCAGCGGGG AGAAAATGAG 
GGATTTGGTT TCGTCATCGT ATCATCCGTC TCCCGGCCTG ATGCTGGAAC CACATTGGCT 
GGAAATGCAT GCGTTGCGAT GCCCCACAAA ATAGGTCGTA TCATAGAGGG CAGCCCGGCT 
GACCGCTGCG GGAAGCTGAA GGTTGGAGAC CGGATTCTGG CTGTCAATGG CTGCTCCATC 
ACAAACAAGT CACATTCGGA CATCGTCAAC CTGATCAAGG AGGCCGGGAA CACTGTCTCA 
CTCAGGATCA TTCCAGGCGA TGAATCTTCC AATGCTTCTC TCCTGACTAA TGCAGAGAAA 
ATCGCTACCA TCACCACCAC ACACACACCT CAAACCACAG AGTTAAGGAA TCATTCAAAG 
TCAAAAGGAG TTCCTCCTCC ACCTCCTTCG CAAACTCATC TGCAGGATGC AGAATACTAT 
TCTGTGGACC TTGAACGGGA CAGTAAAGGC TTCGGCTTCA GCCTAAGAGG AGGACGGGAG 
TACAACATGG ACCTTTATGT GCTGAGACTA GCCGAGGATG GGGCTGCTGT CAGAAATGGC 
AAAATGAGGG TGGGAGATGA GATCCTGGAG ATTAATGGTG AGAGCACAAA GAACATGAAG 
CATTCACGAG CCATTGAACT GATCAAGAAT GGAGGTCGGA GGGCAAGGCT TGTCCTGAAG 
AGAGGAGATG GATCTGTACC CGAGTATGAC GGCAGCAGTG ACGGGTACCC TTCCCCATCC 
GGGGCACAAA ACGCTCCGGA AGTGAGAAAG CTGCCGCCCA ACAGCCGATA TCACACCTCA 
TTTGAGTCCA GTTATCCACC AGACTTTCAC AAACCATCAC GCCTGGAGGA GCCTTCGCGT 
GGCCGAAGTA TGGAGAAGAA CAGGGACTGG GCCGGCTCAG ATCTGATGGA CTTCCATGGC 
CGGCAGTTTA ACCCCCACCT TCATCACACC TGGAATAACA GTCACAGTCA AAGTATCCCC 
CGACAGCAGT CTCCCGACAA CAGTAATGGC AGCACCGTTG GCAACAAGAA GAGCCGAGGA 
CGCAAAGTCA AGAAGTCTGA ATCAGAGAGT GGCATCTCCA AGCTCCTGAA AAGTCTAAGG 
AAAGACAGCT CAGGGAAGAA GGCTGCTGCG GCCGAAAAAC TGCGAGGCAG AGAGCTCTCG 
AGCAGCAGTT CTACTCTGGA CAGTAAACTC CGTCCACAGC GTCGTGGCTC CCTTGATCGC 
TCACCAACCA GGAAGCGTAA CTCATCTCCG GATCGTCGGC GGGCAAAGTC CATGGACCGC 
AGAGCACATC GGGACCACAC ACCTGAGAGG GAATATGATG ACGGAGGGTT TCTCTCTGTA 
ACACCTGAGA GAAAGCTCTA TGAACGGAAG CTCCTCAAGG AGTCACCGTT AGCCGGTCGA 
GTCAGTGAGG TCTCCACCCC TGAGCGAGTC AACAACAGTG CAGATTCTCT CACCAGAGGC 
CGGGCATACG ATAAAGCCAC TAAGAACGGA AACCTTTTGA GAGACTCTTC CTCAGAAAGG 
AGGGAGGAGA GGCACAGAGG GAATGGGACA ATGGAGAGGC GGTACAGACT GGAGCGGGAC 
TCTTCCCCTG ACAGCAACTA CAGTCGGGAC GAGCTGAACT ATGCAGCAGC AGCAGCACCC 
AGACTGAAGG ACTACTACAG CATGATGAAG AACAATGCTG CACACAACAA CGCTACCTAC 
AACAAGACAG GACACAACAA TACTGCTGGA AGACGCAGCT CAAACCAGAT CCCTGAGCCC 
AAGAGAAGGC TGTACAAAGA AAACCCAAAA GACCTCAGTA TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107090390 ( XM_015383730.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015239216.1 Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X4, mRNA.

Price & Availability↑

CloneID	OCy14727
Clone ID Related Accession (Same CDS sequence)	XM_015383730.1
Accession Version	XM_015383730.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4827bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X4
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150746.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACAA AAGTAAATGA GTGCACGATT 
ATCCGGGACA CGAGTGGGGA GTTGAACGTG GAGCTGCACG GTGGAGCGGA GAATGGAGAG 
TTTACATACA TTGGAGATAT CAGGGAGGAT GCTGTGGTTT ATCAGGACGG AAAGCTGTCC 
AAGGGAGAAC TACTGCTAGA CGTGGAGGGT CTGTCGGTGT CTGGATTACC CCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAGGGA CCTGTCAGGT TTAAAACTGT TCGACAAGGC 
AACAAGCTCA ACAAGGACCT AAAGCACTTC TTGAGCTTAA GGTTTCAGAA AACATCACCT 
GACCACGAGT TGCAGAAAAC CATCCGAGCC AATCTATATC GCCATGCTGT GCCTTGTACG 
ACCCGCCCCC CTCGAGACGG AGAGGTCCCA GGGTTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTGGAGGA CAGTGGCACA CTACTGGAAG TAGGAACATA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC TCCAAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
GCTGGCTCCC TTCCGTCCAC ACCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAAAGTC 
CGCGTAGTGC CTGCTGACAA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGATCCT TAGTGCGTAG TCTCTTCCCC TCTCCTTTGC TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGCAG CGGCATCCTC CGGCCGTATC CCGTGCGTGA TAATGCTCTA 
CCCTGTGGTC TGAACAATGC AGCCATTTCC ACTGCAGAGA GCGGGCAGCA GACTCTTGCA 
GAGCCCCAGG TCTCACCAGA GGACCCACTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACAGAGA ACGGGGAACT GTACTTCATA GACACGACAA AGACTCTCCA CCAGGACATG 
GCTGTGTGTT TTCAGAGCTT CCACAACACA AAGACCACTT CATGGCTGGA TCCCCGGTGT 
AGAGACAAAG TCCCCAGGCC TCTGGAAGAG TGTGATGATG ATGAAGAAGG GATACATGCG 
GAAGACCTGA AAAGTGATCT AGAACTGCCT CCAGGATGGG AGAGAATAGA TGACCCAGTG 
TACGGAGTGT ATTATGTGGA CCATATAAAC AGGAAGACCC AGTATGAGAA CCCAGTTTTG 
GAGGCGCGGC ATCAAAAAAT CTTGGAGCAG CAGCAACCGC CGCAGCCTCC AGAAGGTGAG 
CGGTATATCC GAGAGTGGAT AGAGGAGCAT TCGTCTGCTG CAGCTCCATT GGCAAACTAT 
GTTCCAAACC ACCTTGAGAC ATACAGGGAC CCACAAGTCC CACCAGCTCT ACCTCCTGGC 
CCAGTCGGGG TTAAAAGGGG GAAGCCTTTC TTCACCAGAA ATCCTGCAGA ACTGAAAGGA 
ACCTTTATCA ACACCAAGCT AAAGAAAAGT CGCCGTGGAT TTGGCTTCAC AGTCGTTGGT 
GGCGATGAGC CTGATGAATT CCTGCAGATC AAAAGTCTGG TGCTGGACGG GCCTGCAGCT 
GTCGATGGCA AAATGGAGAC AGGGGATGTG ATTGTCAGTG TAAATGACAC CATCGTTCTG 
GGCTACACAC ATGCACAAGT GGTGAAGATT TTCCAGTCCA TCCCTATCGG CTCAATGGTG 
GACCTGGCCT TATGTCGAGG ATATCCGCTG CCATTTGACC CAGATGACCC AAACACCAGC 
CTAGTGACCT CTGTGGCGAT CTTGGACAAG GAGCCTATCA TTGTCAATGG ACAAGAGACT 
TTTGATGTAC CCGCCAACCA GGCTGGACCC ATCAATGGCA TGCGGGAGGC ACGGTCGCAC 
AGCCCCTCAG CGGAGGTGGC ATCAAACGGT TCACATGGGT ACTCCGCTGA CGTGGTCACC 
CTGGCCTCAG CCATCGCCAC CCAGCCAGAG CTGATCACCA TACACATGGA GAAGGGAGAC 
AAAGGGTTTG GCTTCACCAT TGCTGACAGC CTGACAGGAG GCGGGCAGAG AGTCAAGCAG 
ATAGTGGACT ACCCACGGTG CCGTGGCCTG AAAGAGGGGG ACATTCTGAT GGAGGTTAAC 
AAGAGAAACG TCCAGAATAT GAGCCACAAC CAGGTGGTTG ATCTGCTCAG CAAATGTCCG 
AGAGGCAGCG AAGTCACCAT GTTGGTGCAA AGAGGCGTGG TGCCAGCCAA GAGAAGCCCA 
AAGCTGGTGC ACCAGTTGGA GAGAAAGGAC AGTCAGACCA GCTCCCAGCA CAGCGTTTGC 
AGCCATCGAA GTGCTCACAC CGACTCTCCA AGCCACCCAC CCTCTGCCAT GCCAAGTGAG 
GCAGTGGTCC CCACTCCTGC TGCTCCCACA CAGCCTCTGC CGGTCTTGCC CCCCCAAGAC 
CCAGCAGATG GCACTCTCAC CCTCCAGAAG AAGCCAGACC CATTCAAGAT CTGGGCCCAG 
TCCAGAAGCA TGTATGAAAG TCGATTGCCA GATTGCCAGG AGCAAGACAT TTTCCTTTGG 
AGGAAGGATA CTGGCTTTGG CTTCCGCATT TTAGGAGGAA ATGAGCCTGG AGAACCTATT 
TACATTGGTC ATATAGTGAA ATATGGTGCT GCGGATGAAG ATGGCCGCCT GCGGTCTGGT 
GATGAGCTTA TTTGTGTGGA CGGCACAGCG GTAGTGGGCA AGTCCCACCA GCTGGTGGTT 
CAGCTGATGC AGCAGGCTGC CAAACAGGGT CATGTAAACC TCACCGTCAG GCGCAAGACC 
CCCGGATATG GAGTGTCAAA GGGAGAGGGT GATGTTCCGC CATCGCCGGC CTCTTCCCAT 
CACAGCAGCA CTCAGGCGCC AAGCCTCACA GAAGGCAAGC GGACCCCTCA GGGAAGCCAA 
AACTCCCTGA ATACTGTTAG CTCTGGCAGT GGATCTACCA GCGGTATAGG CAGTGGTGGT 
GGAGGTGGCA GTGGAAGCGC TGTGGTGCCC GCCTCTCTCC AGCCATACGA CGTAGAGATC 
CAGCGGGGAG AAAATGAGGG ATTTGGTTTC GTCATCGTAT CATCCGTCTC CCGGCCTGAT 
GCTGGAACCA CATTGGGTCG TATCATAGAG GGCAGCCCGG CTGACCGCTG CGGGAAGCTG 
AAGGTTGGAG ACCGGATTCT GGCTGTCAAT GGCTGCTCCA TCACAAACAA GTCACATTCG 
GACATCGTCA ACCTGATCAA GGAGGCCGGG AACACTGTCT CACTCAGGAT CATTCCAGGC 
GATGAATCTT CCAATGCTTC TCTCCTGACT AATGCAGAGA AAATCGCTAC CATCACCACC 
ACACACACAC CTCAAACCAC AGAGTTAAGG AATCATTCAA AGTCAAAAGG AGTTCCTCCT 
CCACCTCCTT CGCAAACTCA TCTGCAGGAT GCAGAATACT ATTCTGTGGA CCTTGAACGG 
GACAGTAAAG GCTTCGGCTT CAGCCTAAGA GGAGGACGGG AGTACAACAT GGACCTTTAT 
GTGCTGAGAC TAGCCGAGGA TGGGGCTGCT GTCAGAAATG GCAAAATGAG GGTGGGAGAT 
GAGATCCTGG AGATTAATGG TGAGAGCACA AAGAACATGA AGCATTCACG AGCCATTGAA 
CTGATCAAGA ATGGAGGTCG GAGGGCAAGG CTTGTCCTGA AGAGAGGAGA TGGATCTGTA 
CCCGAGTATG ACGGCAGCAG TGACGGGTAC CCTTCCCCAT CCGGGGCACA AAACGCTCCG 
GAAGTGAGAA AGCTGCCGCC CAACAGCCGA TATCACACCT CATTTGAGTC CAGTTATCCA 
CCAGACTTTC ACAAACCATC ACGCCTGGAG GAGCCTTCGC GTGGCCGAAG TATGGAGAAG 
AACAGGGACT GGGCCGGCTC AGATCTGATG GACTTCCATG GCCGGCAGTT TAACCCCCAC 
CTTCATCACA CCTGGAATAA CAGTCACAGT CAAAGTATCC CCCGACAGCA GTCTCCCGAC 
AACAGTAATG GCAGCACCGT TGGCAACAAG AAGAGCCGAG GACGCAAAGT CAAGAAGTCT 
GAATCAGAGA GTGGCATCTC CAAGCTCCTG AAAAGTCTAA GGAAAGACAG CTCAGGGAAG 
AAGGCTGCTG CGGCCGAAAA ACTGCGAGGC AGAGAGCTCT CGAGCAGCAG TTCTACTCTG 
GACAGTAAAC TCCGTCCACA GCGTCGTGGC TCCCTTGATC GCTCACCAAC CAGGAAGCGT 
AACTCATCTC CGGATCGTCG GCGGGCAAAG TCCATGGACC GCAGAGCACA TCGGGACCAC 
ACACCTGAGA GGGAATATGA TGACGGAGGG TTTCTCTCTG TAACACCTGA GAGAAAGCTC 
TATGAACGGA AGCTCCTCAA GGAGTCACCG TTAGCCGGTC GAGTCAGTGA GGTCTCCACC 
CCTGAGCGAG TCAACAACAG TGCAGATTCT CTCACCAGAG GCCGGGCATA CGATAAAGCC 
ACTAAGAACG GAAACCTTTT GAGAGACTCT TCCTCAGAAA GGAGGGAGGA GAGGCACAGA 
GGGAATGGGA CAATGGAGAG GCGGTACAGA CTGGAGCGGG ACTCTTCCCC TGACAGCAAC 
TACAGTCGGG ACGAGCTGAA CTATGCAGCA GCAGCAGCAC CCAGACTGAA GGACTACTAC 
AGCATGATGA AGAACAATGC TGCACACAAC AACGCTACCT ACAACAAGAC AGGACACAAC 
AATACTGCTG GAAGACGCAG CTCAAACCAG ATCCCTGAGC CCAAGAGAAG GCTGTACAAA 
GAAAACCCAA AAGACCTCAG TATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015239216.1
CDS	578..5404
Translation	MAKVIQKKNHWITKVNECTIIRDTSGELNVELHGGAENGEFTYIGDIREDAVVYQDGKLSKGELLLDVEGLSVSGLPLYDIYTVINCCKGPVRFKTVRQGNKLNKDLKHFLSLRFQKTSPDHELQKTIRANLYRHAVPCTTRPPRDGEVPGLDYNFLSVEDFLQLEDSGTLLEVGTYEGNYYGTPKPPRQPIIGTVTLSDAGSLPSTPKRTKSYNDMQKVRVVPADNDDDDQATEMNNSFTGSLVRSLFPSPLLCTGNPNDQDESRSGILRPYPVRDNALPCGLNNAAISTAESGQQTLAEPQVSPEDPLGPLPENWEMAYTENGELYFIDTTKTLHQDMAVCFQSFHNTKTTSWLDPRCRDKVPRPLEECDDDEEGIHAEDLKSDLELPPGWERIDDPVYGVYYVDHINRKTQYENPVLEARHQKILEQQQPPQPPEGERYIREWIEEHSSAAAPLANYVPNHLETYRDPQVPPALPPGPVGVKRGKPFFTRNPAELKGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAAVDGKMETGDVIVSVNDTIVLGYTHAQVVKIFQSIPIGSMVDLALCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETFDVPANQAGPINGMREARSHSPSAEVASNGSHGYSADVVTLASAIATQPELITIHMEKGDKGFGFTIADSLTGGGQRVKQIVDYPRCRGLKEGDILMEVNKRNVQNMSHNQVVDLLSKCPRGSEVTMLVQRGVVPAKRSPKLVHQLERKDSQTSSQHSVCSHRSAHTDSPSHPPSAMPSEAVVPTPAAPTQPLPVLPPQDPADGTLTLQKKPDPFKIWAQSRSMYESRLPDCQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTPGYGVSKGEGDVPPSPASSHHSSTQAPSLTEGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGSAVVPASLQPYDVEIQRGENEGFGFVIVSSVSRPDAGTTLGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVSLRIIPGDESSNASLLTNAEKIATITTTHTPQTTELRNHSKSKGVPPPPPSQTHLQDAEYYSVDLERDSKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKNMKHSRAIELIKNGGRRARLVLKRGDGSVPEYDGSSDGYPSPSGAQNAPEVRKLPPNSRYHTSFESSYPPDFHKPSRLEEPSRGRSMEKNRDWAGSDLMDFHGRQFNPHLHHTWNNSHSQSIPRQQSPDNSNGSTVGNKKSRGRKVKKSESESGISKLLKSLRKDSSGKKAAAAEKLRGRELSSSSSTLDSKLRPQRRGSLDRSPTRKRNSSPDRRRAKSMDRRAHRDHTPEREYDDGGFLSVTPERKLYERKLLKESPLAGRVSEVSTPERVNNSADSLTRGRAYDKATKNGNLLRDSSSERREERHRGNGTMERRYRLERDSSPDSNYSRDELNYAAAAAPRLKDYYSMMKNNAAHNNATYNKTGHNNTAGRRSSNQIPEPKRRLYKENPKDLSI

Target ORF information:

RefSeq Version	XM_015383730.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X4, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015383730.1

ORF Insert Sequence:

ATGGCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACAA AAGTAAATGA GTGCACGATT 
ATCCGGGACA CGAGTGGGGA GTTGAACGTG GAGCTGCACG GTGGAGCGGA GAATGGAGAG 
TTTACATACA TTGGAGATAT CAGGGAGGAT GCTGTGGTTT ATCAGGACGG AAAGCTGTCC 
AAGGGAGAAC TACTGCTAGA CGTGGAGGGT CTGTCGGTGT CTGGATTACC CCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAGGGA CCTGTCAGGT TTAAAACTGT TCGACAAGGC 
AACAAGCTCA ACAAGGACCT AAAGCACTTC TTGAGCTTAA GGTTTCAGAA AACATCACCT 
GACCACGAGT TGCAGAAAAC CATCCGAGCC AATCTATATC GCCATGCTGT GCCTTGTACG 
ACCCGCCCCC CTCGAGACGG AGAGGTCCCA GGGTTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTGGAGGA CAGTGGCACA CTACTGGAAG TAGGAACATA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC TCCAAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
GCTGGCTCCC TTCCGTCCAC ACCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAAAGTC 
CGCGTAGTGC CTGCTGACAA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGATCCT TAGTGCGTAG TCTCTTCCCC TCTCCTTTGC TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGCAG CGGCATCCTC CGGCCGTATC CCGTGCGTGA TAATGCTCTA 
CCCTGTGGTC TGAACAATGC AGCCATTTCC ACTGCAGAGA GCGGGCAGCA GACTCTTGCA 
GAGCCCCAGG TCTCACCAGA GGACCCACTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACAGAGA ACGGGGAACT GTACTTCATA GACACGACAA AGACTCTCCA CCAGGACATG 
GCTGTGTGTT TTCAGAGCTT CCACAACACA AAGACCACTT CATGGCTGGA TCCCCGGTGT 
AGAGACAAAG TCCCCAGGCC TCTGGAAGAG TGTGATGATG ATGAAGAAGG GATACATGCG 
GAAGACCTGA AAAGTGATCT AGAACTGCCT CCAGGATGGG AGAGAATAGA TGACCCAGTG 
TACGGAGTGT ATTATGTGGA CCATATAAAC AGGAAGACCC AGTATGAGAA CCCAGTTTTG 
GAGGCGCGGC ATCAAAAAAT CTTGGAGCAG CAGCAACCGC CGCAGCCTCC AGAAGGTGAG 
CGGTATATCC GAGAGTGGAT AGAGGAGCAT TCGTCTGCTG CAGCTCCATT GGCAAACTAT 
GTTCCAAACC ACCTTGAGAC ATACAGGGAC CCACAAGTCC CACCAGCTCT ACCTCCTGGC 
CCAGTCGGGG TTAAAAGGGG GAAGCCTTTC TTCACCAGAA ATCCTGCAGA ACTGAAAGGA 
ACCTTTATCA ACACCAAGCT AAAGAAAAGT CGCCGTGGAT TTGGCTTCAC AGTCGTTGGT 
GGCGATGAGC CTGATGAATT CCTGCAGATC AAAAGTCTGG TGCTGGACGG GCCTGCAGCT 
GTCGATGGCA AAATGGAGAC AGGGGATGTG ATTGTCAGTG TAAATGACAC CATCGTTCTG 
GGCTACACAC ATGCACAAGT GGTGAAGATT TTCCAGTCCA TCCCTATCGG CTCAATGGTG 
GACCTGGCCT TATGTCGAGG ATATCCGCTG CCATTTGACC CAGATGACCC AAACACCAGC 
CTAGTGACCT CTGTGGCGAT CTTGGACAAG GAGCCTATCA TTGTCAATGG ACAAGAGACT 
TTTGATGTAC CCGCCAACCA GGCTGGACCC ATCAATGGCA TGCGGGAGGC ACGGTCGCAC 
AGCCCCTCAG CGGAGGTGGC ATCAAACGGT TCACATGGGT ACTCCGCTGA CGTGGTCACC 
CTGGCCTCAG CCATCGCCAC CCAGCCAGAG CTGATCACCA TACACATGGA GAAGGGAGAC 
AAAGGGTTTG GCTTCACCAT TGCTGACAGC CTGACAGGAG GCGGGCAGAG AGTCAAGCAG 
ATAGTGGACT ACCCACGGTG CCGTGGCCTG AAAGAGGGGG ACATTCTGAT GGAGGTTAAC 
AAGAGAAACG TCCAGAATAT GAGCCACAAC CAGGTGGTTG ATCTGCTCAG CAAATGTCCG 
AGAGGCAGCG AAGTCACCAT GTTGGTGCAA AGAGGCGTGG TGCCAGCCAA GAGAAGCCCA 
AAGCTGGTGC ACCAGTTGGA GAGAAAGGAC AGTCAGACCA GCTCCCAGCA CAGCGTTTGC 
AGCCATCGAA GTGCTCACAC CGACTCTCCA AGCCACCCAC CCTCTGCCAT GCCAAGTGAG 
GCAGTGGTCC CCACTCCTGC TGCTCCCACA CAGCCTCTGC CGGTCTTGCC CCCCCAAGAC 
CCAGCAGATG GCACTCTCAC CCTCCAGAAG AAGCCAGACC CATTCAAGAT CTGGGCCCAG 
TCCAGAAGCA TGTATGAAAG TCGATTGCCA GATTGCCAGG AGCAAGACAT TTTCCTTTGG 
AGGAAGGATA CTGGCTTTGG CTTCCGCATT TTAGGAGGAA ATGAGCCTGG AGAACCTATT 
TACATTGGTC ATATAGTGAA ATATGGTGCT GCGGATGAAG ATGGCCGCCT GCGGTCTGGT 
GATGAGCTTA TTTGTGTGGA CGGCACAGCG GTAGTGGGCA AGTCCCACCA GCTGGTGGTT 
CAGCTGATGC AGCAGGCTGC CAAACAGGGT CATGTAAACC TCACCGTCAG GCGCAAGACC 
CCCGGATATG GAGTGTCAAA GGGAGAGGGT GATGTTCCGC CATCGCCGGC CTCTTCCCAT 
CACAGCAGCA CTCAGGCGCC AAGCCTCACA GAAGGCAAGC GGACCCCTCA GGGAAGCCAA 
AACTCCCTGA ATACTGTTAG CTCTGGCAGT GGATCTACCA GCGGTATAGG CAGTGGTGGT 
GGAGGTGGCA GTGGAAGCGC TGTGGTGCCC GCCTCTCTCC AGCCATACGA CGTAGAGATC 
CAGCGGGGAG AAAATGAGGG ATTTGGTTTC GTCATCGTAT CATCCGTCTC CCGGCCTGAT 
GCTGGAACCA CATTGGGTCG TATCATAGAG GGCAGCCCGG CTGACCGCTG CGGGAAGCTG 
AAGGTTGGAG ACCGGATTCT GGCTGTCAAT GGCTGCTCCA TCACAAACAA GTCACATTCG 
GACATCGTCA ACCTGATCAA GGAGGCCGGG AACACTGTCT CACTCAGGAT CATTCCAGGC 
GATGAATCTT CCAATGCTTC TCTCCTGACT AATGCAGAGA AAATCGCTAC CATCACCACC 
ACACACACAC CTCAAACCAC AGAGTTAAGG AATCATTCAA AGTCAAAAGG AGTTCCTCCT 
CCACCTCCTT CGCAAACTCA TCTGCAGGAT GCAGAATACT ATTCTGTGGA CCTTGAACGG 
GACAGTAAAG GCTTCGGCTT CAGCCTAAGA GGAGGACGGG AGTACAACAT GGACCTTTAT 
GTGCTGAGAC TAGCCGAGGA TGGGGCTGCT GTCAGAAATG GCAAAATGAG GGTGGGAGAT 
GAGATCCTGG AGATTAATGG TGAGAGCACA AAGAACATGA AGCATTCACG AGCCATTGAA 
CTGATCAAGA ATGGAGGTCG GAGGGCAAGG CTTGTCCTGA AGAGAGGAGA TGGATCTGTA 
CCCGAGTATG ACGGCAGCAG TGACGGGTAC CCTTCCCCAT CCGGGGCACA AAACGCTCCG 
GAAGTGAGAA AGCTGCCGCC CAACAGCCGA TATCACACCT CATTTGAGTC CAGTTATCCA 
CCAGACTTTC ACAAACCATC ACGCCTGGAG GAGCCTTCGC GTGGCCGAAG TATGGAGAAG 
AACAGGGACT GGGCCGGCTC AGATCTGATG GACTTCCATG GCCGGCAGTT TAACCCCCAC 
CTTCATCACA CCTGGAATAA CAGTCACAGT CAAAGTATCC CCCGACAGCA GTCTCCCGAC 
AACAGTAATG GCAGCACCGT TGGCAACAAG AAGAGCCGAG GACGCAAAGT CAAGAAGTCT 
GAATCAGAGA GTGGCATCTC CAAGCTCCTG AAAAGTCTAA GGAAAGACAG CTCAGGGAAG 
AAGGCTGCTG CGGCCGAAAA ACTGCGAGGC AGAGAGCTCT CGAGCAGCAG TTCTACTCTG 
GACAGTAAAC TCCGTCCACA GCGTCGTGGC TCCCTTGATC GCTCACCAAC CAGGAAGCGT 
AACTCATCTC CGGATCGTCG GCGGGCAAAG TCCATGGACC GCAGAGCACA TCGGGACCAC 
ACACCTGAGA GGGAATATGA TGACGGAGGG TTTCTCTCTG TAACACCTGA GAGAAAGCTC 
TATGAACGGA AGCTCCTCAA GGAGTCACCG TTAGCCGGTC GAGTCAGTGA GGTCTCCACC 
CCTGAGCGAG TCAACAACAG TGCAGATTCT CTCACCAGAG GCCGGGCATA CGATAAAGCC 
ACTAAGAACG GAAACCTTTT GAGAGACTCT TCCTCAGAAA GGAGGGAGGA GAGGCACAGA 
GGGAATGGGA CAATGGAGAG GCGGTACAGA CTGGAGCGGG ACTCTTCCCC TGACAGCAAC 
TACAGTCGGG ACGAGCTGAA CTATGCAGCA GCAGCAGCAC CCAGACTGAA GGACTACTAC 
AGCATGATGA AGAACAATGC TGCACACAAC AACGCTACCT ACAACAAGAC AGGACACAAC 
AATACTGCTG GAAGACGCAG CTCAAACCAG ATCCCTGAGC CCAAGAGAAG GCTGTACAAA 
GAAAACCCAA AAGACCTCAG TATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107090390 ( XM_015383731.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015239217.1 Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X5, mRNA.

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CloneID	OCy14728
Clone ID Related Accession (Same CDS sequence)	XM_015383731.1
Accession Version	XM_015383731.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4824bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X5
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150746.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACAA AAGTAAATGA GTGCACGATT 
ATCCGGGACA CGAGTGGGGA GTTGAACGTG GAGCTGCACG GTGGAGCGGA GAATGGAGAG 
TTTACATACA TTGGAGATAT CAGGGAGGAT GCTGTGGTTT ATCAGGACGG AAAGCTGTCC 
AAGGGAGAAC TACTGCTAGA CGTGGAGGGT CTGTCGGTGT CTGGATTACC CCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAGGGA CCTGTCAGGT TTAAAACTGT TCGACAAGGC 
AACAAGCTCA ACAAGGACCT AAAGCACTTC TTGAGCTTAA GGTTTCAGAA AACATCACCT 
GACCACGAGT TGCAGAAAAC CATCCGAGCC AATCTATATC GCCATGCTGT GCCTTGTACG 
ACCCGCCCCC CTCGAGACGG AGAGGTCCCA GGGTTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTGGAGGA CAGTGGCACA CTACTGGAAG TAGGAACATA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC TCCAAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
GCTGGCTCCC TTCCGTCCAC ACCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAAAGTC 
CGCGTAGTGC CTGCTGACAA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGATCCT TAGTGCGTAG TCTCTTCCCC TCTCCTTTGC TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGCAG CGGCATCCTC CGGCCGTATC CCGTGCGTGA TAATGCTCTA 
CCCTGTGGTC TGAACAATGC AGCCATTTCC ACTGCAGAGA GCGGGCAGCA GACTCTTGCA 
GAGCCCCAGG TCTCACCAGA GGACCCACTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACAGAGA ACGGGGAACT GTACTTCATA GACACGACAA AGACTCTCCA CCAGGACATG 
GCTGTGTGTT TTCAGAGCTT CCACAACACA AAGACCACTT CATGGCTGGA TCCCCGGTGT 
AGAGACAAAG TCCCCAGGCC TCTGGAAGAG TGTGATGATG ATGAACTGCC TCCAGGATGG 
GAGAGAATAG ATGACCCAGT GTACGGAGTG TATTATGTGG ACCATATAAA CAGGAAGACC 
CAGTATGAGA ACCCAGTTTT GGAGGCGCGG CATCAAAAAA TCTTGGAGCA GCAGCAACCG 
CCGCAGCCTC CAGAAGGTGA GCGGTATATC CGAGAGTGGA TAGAGGAGCA TTCGTCTGCT 
GCAGCTCCAT TGGCAAACTA TGTTCCAAAC CACCTTGAGA CATACAGGGA CCCACAAGTC 
CCACCAGCTC TACCTCCTGG CCCAGTCGGG GTTAAAAGGG GGAAGCCTTT CTTCACCAGA 
AATCCTGCAG AACTGAAAGG AACCTTTATC AACACCAAGC TAAAGAAAAG TCGCCGTGGA 
TTTGGCTTCA CAGTCGTTGG TGGCGATGAG CCTGATGAAT TCCTGCAGAT CAAAAGTCTG 
GTGCTGGACG GGCCTGCAGC TGTCGATGGC AAAATGGAGA CAGGGGATGT GATTGTCAGT 
GTAAATGACA CCATCGTTCT GGGCTACACA CATGCACAAG TGGTGAAGAT TTTCCAGTCC 
ATCCCTATCG GCTCAATGGT GGACCTGGCC TTATGTCGAG GATATCCGCT GCCATTTGAC 
CCAGATGACC CAAACACCAG CCTAGTGACC TCTGTGGCGA TCTTGGACAA GGAGCCTATC 
ATTGTCAATG GACAAGAGAC TTTTGATGTA CCCGCCAACC AGGCTGGACC CATCAATGGC 
ATGCGGGAGG CACGGTCGCA CAGCCCCTCA GCGGAGGTGG CATCAAACGG TTCACATGGG 
TACTCCGCTG ACGTGGTCAC CCTGGCCTCA GCCATCGCCA CCCAGCCAGA GCTGATCACC 
ATACACATGG AGAAGGGAGA CAAAGGGTTT GGCTTCACCA TTGCTGACAG CCTGACAGGA 
GGCGGGCAGA GAGTCAAGCA GATAGTGGAC TACCCACGGT GCCGTGGCCT GAAAGAGGGG 
GACATTCTGA TGGAGGTTAA CAAGAGAAAC GTCCAGAATA TGAGCCACAA CCAGGTGGTT 
GATCTGCTCA GCAAATGTCC GAGAGGCAGC GAAGTCACCA TGTTGGTGCA AAGAGGCGTG 
GTGCCAGCCA AGAGAAGCCC AAAGCTGGTG CACCAGTTGG AGAGAAAGGA CAGTCAGACC 
AGCTCCCAGC ACAGCGTTTG CAGCCATCGA AGTGCTCACA CCGACTCTCC AAGCCACCCA 
CCCTCTGCCA TGCCAAGTGA GGCAGTGGTC CCCACTCCTG CTGCTCCCAC ACAGCCTCTG 
CCGGTCTTGC CCCCCCAAGA CCCAGCAGAT GGCACTCTCA CCCTCCAGAA GAAGCCAGAC 
CCATTCAAGA TCTGGGCCCA GTCCAGAAGC ATGTATGAAA GTCGATTGCC AGATTGCCAG 
GAGCAAGACA TTTTCCTTTG GAGGAAGGAT ACTGGCTTTG GCTTCCGCAT TTTAGGAGGA 
AATGAGCCTG GAGAACCTAT TTACATTGGT CATATAGTGA AATATGGTGC TGCGGATGAA 
GATGGCCGCC TGCGGTCTGG TGATGAGCTT ATTTGTGTGG ACGGCACAGC GGTAGTGGGC 
AAGTCCCACC AGCTGGTGGT TCAGCTGATG CAGCAGGCTG CCAAACAGGG TCATGTAAAC 
CTCACCGTCA GGCGCAAGAC CCCCGGATAT GGAGTGTCAA AGGGAGAGGG TGATGTTCCG 
CCATCGCCGG CCTCTTCCCA TCACAGCAGC ACTCAGGCGC CAAGCCTCAC AGAAGGCAAG 
CGGACCCCTC AGGGAAGCCA AAACTCCCTG AATACTGTTA GCTCTGGCAG TGGATCTACC 
AGCGGTATAG GCAGTGGTGG TGGAGGTGGC AGTGGAAGCG CTGTGGTGCC CGCCTCTCTC 
CAGCCATACG ACGTAGAGAT CCAGCGGGGA GAAAATGAGG GATTTGGTTT CGTCATCGTA 
TCATCCGTCT CCCGGCCTGA TGCTGGAACC ACATTGGCTG GAAATGCATG CGTTGCGATG 
CCCCACAAAA TAGGTCGTAT CATAGAGGGC AGCCCGGCTG ACCGCTGCGG GAAGCTGAAG 
GTTGGAGACC GGATTCTGGC TGTCAATGGC TGCTCCATCA CAAACAAGTC ACATTCGGAC 
ATCGTCAACC TGATCAAGGA GGCCGGGAAC ACTGTCTCAC TCAGGATCAT TCCAGGCGAT 
GAATCTTCCA ATGCTTCTCT CCTGACTAAT GCAGAGAAAA TCGCTACCAT CACCACCACA 
CACACACCTC AAACCACAGA GTTAAGGAAT CATTCAAAGT CAAAAGGAGT TCCTCCTCCA 
CCTCCTTCGC AAACTCATCT GCAGGATGCA GAATACTATT CTGTGGACCT TGAACGGGAC 
AGTAAAGGCT TCGGCTTCAG CCTAAGAGGA GGACGGGAGT ACAACATGGA CCTTTATGTG 
CTGAGACTAG CCGAGGATGG GGCTGCTGTC AGAAATGGCA AAATGAGGGT GGGAGATGAG 
ATCCTGGAGA TTAATGGTGA GAGCACAAAG AACATGAAGC ATTCACGAGC CATTGAACTG 
ATCAAGAATG GAGGTCGGAG GGCAAGGCTT GTCCTGAAGA GAGGAGATGG ATCTGTACCC 
GAGTATGACG GCAGCAGTGA CGGGTACCCT TCCCCATCCG GGGCACAAAA CGCTCCGGAA 
GTGAGAAAGC TGCCGCCCAA CAGCCGATAT CACACCTCAT TTGAGTCCAG TTATCCACCA 
GACTTTCACA AACCATCACG CCTGGAGGAG CCTTCGCGTG GCCGAAGTAT GGAGAAGAAC 
AGGGACTGGG CCGGCTCAGA TCTGATGGAC TTCCATGGCC GGCAGTTTAA CCCCCACCTT 
CATCACACCT GGAATAACAG TCACAGTCAA AGTATCCCCC GACAGCAGTC TCCCGACAAC 
AGTAATGGCA GCACCGTTGG CAACAAGAAG AGCCGAGGAC GCAAAGTCAA GAAGTCTGAA 
TCAGAGAGTG GCATCTCCAA GCTCCTGAAA AGTCTAAGGA AAGACAGCTC AGGGAAGAAG 
GCTGCTGCGG CCGAAAAACT GCGAGGCAGA GAGCTCTCGA GCAGCAGTTC TACTCTGGAC 
AGTAAACTCC GTCCACAGCG TCGTGGCTCC CTTGATCGCT CACCAACCAG GAAGCGTAAC 
TCATCTCCGG ATCGTCGGCG GGCAAAGTCC ATGGACCGCA GAGCACATCG GGACCACACA 
CCTGAGAGGG AATATGATGA CGGAGGGTTT CTCTCTGTAA CACCTGAGAG AAAGCTCTAT 
GAACGGAAGC TCCTCAAGGA GTCACCGTTA GCCGGTCGAG TCAGTGAGGT CTCCACCCCT 
GAGCGAGTCA ACAACAGTGC AGATTCTCTC ACCAGAGGCC GGGCATACGA TAAAGCCACT 
AAGAACGGAA ACCTTTTGAG AGACTCTTCC TCAGAAAGGA GGGAGGAGAG GCACAGAGGG 
AATGGGACAA TGGAGAGGCG GTACAGACTG GAGCGGGACT CTTCCCCTGA CAGCAACTAC 
AGTCGGGACG AGCTGAACTA TGCAGCAGCA GCAGCACCCA GACTGAAGGA CTACTACAGC 
ATGATGAAGA ACAATGCTGC ACACAACAAC GCTACCTACA ACAAGACAGG ACACAACAAT 
ACTGCTGGAA GACGCAGCTC AAACCAGATC CCTGAGCCCA AGAGAAGGCT GTACAAAGAA 
AACCCAAAAG ACCTCAGTAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015239217.1
CDS	576..5399
Translation	MAKVIQKKNHWITKVNECTIIRDTSGELNVELHGGAENGEFTYIGDIREDAVVYQDGKLSKGELLLDVEGLSVSGLPLYDIYTVINCCKGPVRFKTVRQGNKLNKDLKHFLSLRFQKTSPDHELQKTIRANLYRHAVPCTTRPPRDGEVPGLDYNFLSVEDFLQLEDSGTLLEVGTYEGNYYGTPKPPRQPIIGTVTLSDAGSLPSTPKRTKSYNDMQKVRVVPADNDDDDQATEMNNSFTGSLVRSLFPSPLLCTGNPNDQDESRSGILRPYPVRDNALPCGLNNAAISTAESGQQTLAEPQVSPEDPLGPLPENWEMAYTENGELYFIDTTKTLHQDMAVCFQSFHNTKTTSWLDPRCRDKVPRPLEECDDDELPPGWERIDDPVYGVYYVDHINRKTQYENPVLEARHQKILEQQQPPQPPEGERYIREWIEEHSSAAAPLANYVPNHLETYRDPQVPPALPPGPVGVKRGKPFFTRNPAELKGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAAVDGKMETGDVIVSVNDTIVLGYTHAQVVKIFQSIPIGSMVDLALCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETFDVPANQAGPINGMREARSHSPSAEVASNGSHGYSADVVTLASAIATQPELITIHMEKGDKGFGFTIADSLTGGGQRVKQIVDYPRCRGLKEGDILMEVNKRNVQNMSHNQVVDLLSKCPRGSEVTMLVQRGVVPAKRSPKLVHQLERKDSQTSSQHSVCSHRSAHTDSPSHPPSAMPSEAVVPTPAAPTQPLPVLPPQDPADGTLTLQKKPDPFKIWAQSRSMYESRLPDCQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTPGYGVSKGEGDVPPSPASSHHSSTQAPSLTEGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGSAVVPASLQPYDVEIQRGENEGFGFVIVSSVSRPDAGTTLAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVSLRIIPGDESSNASLLTNAEKIATITTTHTPQTTELRNHSKSKGVPPPPPSQTHLQDAEYYSVDLERDSKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKNMKHSRAIELIKNGGRRARLVLKRGDGSVPEYDGSSDGYPSPSGAQNAPEVRKLPPNSRYHTSFESSYPPDFHKPSRLEEPSRGRSMEKNRDWAGSDLMDFHGRQFNPHLHHTWNNSHSQSIPRQQSPDNSNGSTVGNKKSRGRKVKKSESESGISKLLKSLRKDSSGKKAAAAEKLRGRELSSSSSTLDSKLRPQRRGSLDRSPTRKRNSSPDRRRAKSMDRRAHRDHTPEREYDDGGFLSVTPERKLYERKLLKESPLAGRVSEVSTPERVNNSADSLTRGRAYDKATKNGNLLRDSSSERREERHRGNGTMERRYRLERDSSPDSNYSRDELNYAAAAAPRLKDYYSMMKNNAAHNNATYNKTGHNNTAGRRSSNQIPEPKRRLYKENPKDLSI

Target ORF information:

RefSeq Version	XM_015383731.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X5, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015383731.1

ORF Insert Sequence:

ATGGCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACAA AAGTAAATGA GTGCACGATT 
ATCCGGGACA CGAGTGGGGA GTTGAACGTG GAGCTGCACG GTGGAGCGGA GAATGGAGAG 
TTTACATACA TTGGAGATAT CAGGGAGGAT GCTGTGGTTT ATCAGGACGG AAAGCTGTCC 
AAGGGAGAAC TACTGCTAGA CGTGGAGGGT CTGTCGGTGT CTGGATTACC CCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAGGGA CCTGTCAGGT TTAAAACTGT TCGACAAGGC 
AACAAGCTCA ACAAGGACCT AAAGCACTTC TTGAGCTTAA GGTTTCAGAA AACATCACCT 
GACCACGAGT TGCAGAAAAC CATCCGAGCC AATCTATATC GCCATGCTGT GCCTTGTACG 
ACCCGCCCCC CTCGAGACGG AGAGGTCCCA GGGTTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTGGAGGA CAGTGGCACA CTACTGGAAG TAGGAACATA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC TCCAAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
GCTGGCTCCC TTCCGTCCAC ACCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAAAGTC 
CGCGTAGTGC CTGCTGACAA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGATCCT TAGTGCGTAG TCTCTTCCCC TCTCCTTTGC TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGCAG CGGCATCCTC CGGCCGTATC CCGTGCGTGA TAATGCTCTA 
CCCTGTGGTC TGAACAATGC AGCCATTTCC ACTGCAGAGA GCGGGCAGCA GACTCTTGCA 
GAGCCCCAGG TCTCACCAGA GGACCCACTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACAGAGA ACGGGGAACT GTACTTCATA GACACGACAA AGACTCTCCA CCAGGACATG 
GCTGTGTGTT TTCAGAGCTT CCACAACACA AAGACCACTT CATGGCTGGA TCCCCGGTGT 
AGAGACAAAG TCCCCAGGCC TCTGGAAGAG TGTGATGATG ATGAACTGCC TCCAGGATGG 
GAGAGAATAG ATGACCCAGT GTACGGAGTG TATTATGTGG ACCATATAAA CAGGAAGACC 
CAGTATGAGA ACCCAGTTTT GGAGGCGCGG CATCAAAAAA TCTTGGAGCA GCAGCAACCG 
CCGCAGCCTC CAGAAGGTGA GCGGTATATC CGAGAGTGGA TAGAGGAGCA TTCGTCTGCT 
GCAGCTCCAT TGGCAAACTA TGTTCCAAAC CACCTTGAGA CATACAGGGA CCCACAAGTC 
CCACCAGCTC TACCTCCTGG CCCAGTCGGG GTTAAAAGGG GGAAGCCTTT CTTCACCAGA 
AATCCTGCAG AACTGAAAGG AACCTTTATC AACACCAAGC TAAAGAAAAG TCGCCGTGGA 
TTTGGCTTCA CAGTCGTTGG TGGCGATGAG CCTGATGAAT TCCTGCAGAT CAAAAGTCTG 
GTGCTGGACG GGCCTGCAGC TGTCGATGGC AAAATGGAGA CAGGGGATGT GATTGTCAGT 
GTAAATGACA CCATCGTTCT GGGCTACACA CATGCACAAG TGGTGAAGAT TTTCCAGTCC 
ATCCCTATCG GCTCAATGGT GGACCTGGCC TTATGTCGAG GATATCCGCT GCCATTTGAC 
CCAGATGACC CAAACACCAG CCTAGTGACC TCTGTGGCGA TCTTGGACAA GGAGCCTATC 
ATTGTCAATG GACAAGAGAC TTTTGATGTA CCCGCCAACC AGGCTGGACC CATCAATGGC 
ATGCGGGAGG CACGGTCGCA CAGCCCCTCA GCGGAGGTGG CATCAAACGG TTCACATGGG 
TACTCCGCTG ACGTGGTCAC CCTGGCCTCA GCCATCGCCA CCCAGCCAGA GCTGATCACC 
ATACACATGG AGAAGGGAGA CAAAGGGTTT GGCTTCACCA TTGCTGACAG CCTGACAGGA 
GGCGGGCAGA GAGTCAAGCA GATAGTGGAC TACCCACGGT GCCGTGGCCT GAAAGAGGGG 
GACATTCTGA TGGAGGTTAA CAAGAGAAAC GTCCAGAATA TGAGCCACAA CCAGGTGGTT 
GATCTGCTCA GCAAATGTCC GAGAGGCAGC GAAGTCACCA TGTTGGTGCA AAGAGGCGTG 
GTGCCAGCCA AGAGAAGCCC AAAGCTGGTG CACCAGTTGG AGAGAAAGGA CAGTCAGACC 
AGCTCCCAGC ACAGCGTTTG CAGCCATCGA AGTGCTCACA CCGACTCTCC AAGCCACCCA 
CCCTCTGCCA TGCCAAGTGA GGCAGTGGTC CCCACTCCTG CTGCTCCCAC ACAGCCTCTG 
CCGGTCTTGC CCCCCCAAGA CCCAGCAGAT GGCACTCTCA CCCTCCAGAA GAAGCCAGAC 
CCATTCAAGA TCTGGGCCCA GTCCAGAAGC ATGTATGAAA GTCGATTGCC AGATTGCCAG 
GAGCAAGACA TTTTCCTTTG GAGGAAGGAT ACTGGCTTTG GCTTCCGCAT TTTAGGAGGA 
AATGAGCCTG GAGAACCTAT TTACATTGGT CATATAGTGA AATATGGTGC TGCGGATGAA 
GATGGCCGCC TGCGGTCTGG TGATGAGCTT ATTTGTGTGG ACGGCACAGC GGTAGTGGGC 
AAGTCCCACC AGCTGGTGGT TCAGCTGATG CAGCAGGCTG CCAAACAGGG TCATGTAAAC 
CTCACCGTCA GGCGCAAGAC CCCCGGATAT GGAGTGTCAA AGGGAGAGGG TGATGTTCCG 
CCATCGCCGG CCTCTTCCCA TCACAGCAGC ACTCAGGCGC CAAGCCTCAC AGAAGGCAAG 
CGGACCCCTC AGGGAAGCCA AAACTCCCTG AATACTGTTA GCTCTGGCAG TGGATCTACC 
AGCGGTATAG GCAGTGGTGG TGGAGGTGGC AGTGGAAGCG CTGTGGTGCC CGCCTCTCTC 
CAGCCATACG ACGTAGAGAT CCAGCGGGGA GAAAATGAGG GATTTGGTTT CGTCATCGTA 
TCATCCGTCT CCCGGCCTGA TGCTGGAACC ACATTGGCTG GAAATGCATG CGTTGCGATG 
CCCCACAAAA TAGGTCGTAT CATAGAGGGC AGCCCGGCTG ACCGCTGCGG GAAGCTGAAG 
GTTGGAGACC GGATTCTGGC TGTCAATGGC TGCTCCATCA CAAACAAGTC ACATTCGGAC 
ATCGTCAACC TGATCAAGGA GGCCGGGAAC ACTGTCTCAC TCAGGATCAT TCCAGGCGAT 
GAATCTTCCA ATGCTTCTCT CCTGACTAAT GCAGAGAAAA TCGCTACCAT CACCACCACA 
CACACACCTC AAACCACAGA GTTAAGGAAT CATTCAAAGT CAAAAGGAGT TCCTCCTCCA 
CCTCCTTCGC AAACTCATCT GCAGGATGCA GAATACTATT CTGTGGACCT TGAACGGGAC 
AGTAAAGGCT TCGGCTTCAG CCTAAGAGGA GGACGGGAGT ACAACATGGA CCTTTATGTG 
CTGAGACTAG CCGAGGATGG GGCTGCTGTC AGAAATGGCA AAATGAGGGT GGGAGATGAG 
ATCCTGGAGA TTAATGGTGA GAGCACAAAG AACATGAAGC ATTCACGAGC CATTGAACTG 
ATCAAGAATG GAGGTCGGAG GGCAAGGCTT GTCCTGAAGA GAGGAGATGG ATCTGTACCC 
GAGTATGACG GCAGCAGTGA CGGGTACCCT TCCCCATCCG GGGCACAAAA CGCTCCGGAA 
GTGAGAAAGC TGCCGCCCAA CAGCCGATAT CACACCTCAT TTGAGTCCAG TTATCCACCA 
GACTTTCACA AACCATCACG CCTGGAGGAG CCTTCGCGTG GCCGAAGTAT GGAGAAGAAC 
AGGGACTGGG CCGGCTCAGA TCTGATGGAC TTCCATGGCC GGCAGTTTAA CCCCCACCTT 
CATCACACCT GGAATAACAG TCACAGTCAA AGTATCCCCC GACAGCAGTC TCCCGACAAC 
AGTAATGGCA GCACCGTTGG CAACAAGAAG AGCCGAGGAC GCAAAGTCAA GAAGTCTGAA 
TCAGAGAGTG GCATCTCCAA GCTCCTGAAA AGTCTAAGGA AAGACAGCTC AGGGAAGAAG 
GCTGCTGCGG CCGAAAAACT GCGAGGCAGA GAGCTCTCGA GCAGCAGTTC TACTCTGGAC 
AGTAAACTCC GTCCACAGCG TCGTGGCTCC CTTGATCGCT CACCAACCAG GAAGCGTAAC 
TCATCTCCGG ATCGTCGGCG GGCAAAGTCC ATGGACCGCA GAGCACATCG GGACCACACA 
CCTGAGAGGG AATATGATGA CGGAGGGTTT CTCTCTGTAA CACCTGAGAG AAAGCTCTAT 
GAACGGAAGC TCCTCAAGGA GTCACCGTTA GCCGGTCGAG TCAGTGAGGT CTCCACCCCT 
GAGCGAGTCA ACAACAGTGC AGATTCTCTC ACCAGAGGCC GGGCATACGA TAAAGCCACT 
AAGAACGGAA ACCTTTTGAG AGACTCTTCC TCAGAAAGGA GGGAGGAGAG GCACAGAGGG 
AATGGGACAA TGGAGAGGCG GTACAGACTG GAGCGGGACT CTTCCCCTGA CAGCAACTAC 
AGTCGGGACG AGCTGAACTA TGCAGCAGCA GCAGCACCCA GACTGAAGGA CTACTACAGC 
ATGATGAAGA ACAATGCTGC ACACAACAAC GCTACCTACA ACAAGACAGG ACACAACAAT 
ACTGCTGGAA GACGCAGCTC AAACCAGATC CCTGAGCCCA AGAGAAGGCT GTACAAAGAA 
AACCCAAAAG ACCTCAGTAT CTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107090390 ( XM_015383733.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015239219.1 Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X6, mRNA.

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CloneID	OCy14729
Clone ID Related Accession (Same CDS sequence)	XM_015383733.1
Accession Version	XM_015383733.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4818bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X6
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150746.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACAA AAGTAAATGA GTGCACGATT 
ATCCGGGACA CGAGTGGGGA GTTGAACGTG GAGCTGCACG GTGGAGCGGA GAATGGAGAG 
TTTACATACA TTGGAGATAT CAGGGAGGAT GCTGTGGTTT ATCAGGACGG AAAGCTGTCC 
AAGGGAGAAC TACTGCTAGA CGTGGAGGGT CTGTCGGTGT CTGGATTACC CCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAGGGA CCTGTCAGGT TTAAAACTGT TCGACAAGGC 
AACAAGCTCA ACAAGGACCT AAAGCACTTC TTGAGCTTAA GGTTTCAGAA AACATCACCT 
GACCACGAGT TGCAGAAAAC CATCCGAGCC AATCTATATC GCCATGCTGT GCCTTGTACG 
ACCCGCCCCC CTCGAGACGG AGAGGTCCCA GGGTTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTGGAGGA CAGTGGCACA CTACTGGAAG TAGGAACATA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC TCCAAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
GCTGGCTCCC TTCCGTCCAC ACCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAAAGTC 
CGCGTAGTGC CTGCTGACAA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGTAACC CTAACGACCA GGACGAGAGC CGCAGCGGCA TCCTCCGGCC GTATCCCGTG 
CGTGATAATG CTCTACCCTG TGGTCTGAAC AATGCAGCCA TTTCCACTGC AGAGAGCGGG 
CAGCAGACTC TTGCAGAGCC CCAGGTCTCA CCAGAGGACC CACTGGGACC GCTGCCTGAA 
AACTGGGAGA TGGCCTACAC AGAGAACGGG GAACTGTACT TCATAGACAC GACAAAGACT 
CTCCACCAGG ACATGGCTGT GTGTTTTCAG AGCTTCCACA ACACAAAGAC CACTTCATGG 
CTGGATCCCC GGTGTAGAGA CAAAGTCCCC AGGCCTCTGG AAGAGTGTGA TGATGATGAA 
GAAGGGATAC ATGCGGAAGA CCTGAAAAGT GATCTAGAAC TGCCTCCAGG ATGGGAGAGA 
ATAGATGACC CAGTGTACGG AGTGTATTAT GTGGACCATA TAAACAGGAA GACCCAGTAT 
GAGAACCCAG TTTTGGAGGC GCGGCATCAA AAAATCTTGG AGCAGCAGCA ACCGCCGCAG 
CCTCCAGAAG GTGAGCGGTA TATCCGAGAG TGGATAGAGG AGCATTCGTC TGCTGCAGCT 
CCATTGGCAA ACTATGTTCC AAACCACCTT GAGACATACA GGGACCCACA AGTCCCACCA 
GCTCTACCTC CTGGCCCAGT CGGGGTTAAA AGGGGGAAGC CTTTCTTCAC CAGAAATCCT 
GCAGAACTGA AAGGAACCTT TATCAACACC AAGCTAAAGA AAAGTCGCCG TGGATTTGGC 
TTCACAGTCG TTGGTGGCGA TGAGCCTGAT GAATTCCTGC AGATCAAAAG TCTGGTGCTG 
GACGGGCCTG CAGCTGTCGA TGGCAAAATG GAGACAGGGG ATGTGATTGT CAGTGTAAAT 
GACACCATCG TTCTGGGCTA CACACATGCA CAAGTGGTGA AGATTTTCCA GTCCATCCCT 
ATCGGCTCAA TGGTGGACCT GGCCTTATGT CGAGGATATC CGCTGCCATT TGACCCAGAT 
GACCCAAACA CCAGCCTAGT GACCTCTGTG GCGATCTTGG ACAAGGAGCC TATCATTGTC 
AATGGACAAG AGACTTTTGA TGTACCCGCC AACCAGGCTG GACCCATCAA TGGCATGCGG 
GAGGCACGGT CGCACAGCCC CTCAGCGGAG GTGGCATCAA ACGGTTCACA TGGGTACTCC 
GCTGACGTGG TCACCCTGGC CTCAGCCATC GCCACCCAGC CAGAGCTGAT CACCATACAC 
ATGGAGAAGG GAGACAAAGG GTTTGGCTTC ACCATTGCTG ACAGCCTGAC AGGAGGCGGG 
CAGAGAGTCA AGCAGATAGT GGACTACCCA CGGTGCCGTG GCCTGAAAGA GGGGGACATT 
CTGATGGAGG TTAACAAGAG AAACGTCCAG AATATGAGCC ACAACCAGGT GGTTGATCTG 
CTCAGCAAAT GTCCGAGAGG CAGCGAAGTC ACCATGTTGG TGCAAAGAGG CGTGGTGCCA 
GCCAAGAGAA GCCCAAAGCT GGTGCACCAG TTGGAGAGAA AGGACAGTCA GACCAGCTCC 
CAGCACAGCG TTTGCAGCCA TCGAAGTGCT CACACCGACT CTCCAAGCCA CCCACCCTCT 
GCCATGCCAA GTGAGGCAGT GGTCCCCACT CCTGCTGCTC CCACACAGCC TCTGCCGGTC 
TTGCCCCCCC AAGACCCAGC AGATGGCACT CTCACCCTCC AGAAGAAGCC AGACCCATTC 
AAGATCTGGG CCCAGTCCAG AAGCATGTAT GAAAGTCGAT TGCCAGATTG CCAGGAGCAA 
GACATTTTCC TTTGGAGGAA GGATACTGGC TTTGGCTTCC GCATTTTAGG AGGAAATGAG 
CCTGGAGAAC CTATTTACAT TGGTCATATA GTGAAATATG GTGCTGCGGA TGAAGATGGC 
CGCCTGCGGT CTGGTGATGA GCTTATTTGT GTGGACGGCA CAGCGGTAGT GGGCAAGTCC 
CACCAGCTGG TGGTTCAGCT GATGCAGCAG GCTGCCAAAC AGGGTCATGT AAACCTCACC 
GTCAGGCGCA AGACCCCCGG ATATGGAGTG TCAAAGGGAG AGGGTGATGT TCCGCCATCG 
CCGGCCTCTT CCCATCACAG CAGCACTCAG GCGCCAAGCC TCACAGAAGG CAAGCGGACC 
CCTCAGGGAA GCCAAAACTC CCTGAATACT GTTAGCTCTG GCAGTGGATC TACCAGCGGT 
ATAGGCAGTG GTGGTGGAGG TGGCAGTGGA AGCGCTGTGG TGCCCGCCTC TCTCCAGCCA 
TACGACGTAG AGATCCAGCG GGGAGAAAAT GAGGGATTTG GTTTCGTCAT CGTATCATCC 
GTCTCCCGGC CTGATGCTGG AACCACATTG GCTGGAAATG CATGCGTTGC GATGCCCCAC 
AAAATAGGTC GTATCATAGA GGGCAGCCCG GCTGACCGCT GCGGGAAGCT GAAGGTTGGA 
GACCGGATTC TGGCTGTCAA TGGCTGCTCC ATCACAAACA AGTCACATTC GGACATCGTC 
AACCTGATCA AGGAGGCCGG GAACACTGTC TCACTCAGGA TCATTCCAGG CGATGAATCT 
TCCAATGCTT CTCTCCTGAC TAATGCAGAG AAAATCGCTA CCATCACCAC CACACACACA 
CCTCAAACCA CAGAGTTAAG GAATCATTCA AAGTCAAAAG GAGTTCCTCC TCCACCTCCT 
TCGCAAACTC ATCTGCAGGA TGCAGAATAC TATTCTGTGG ACCTTGAACG GGACAGTAAA 
GGCTTCGGCT TCAGCCTAAG AGGAGGACGG GAGTACAACA TGGACCTTTA TGTGCTGAGA 
CTAGCCGAGG ATGGGGCTGC TGTCAGAAAT GGCAAAATGA GGGTGGGAGA TGAGATCCTG 
GAGATTAATG GTGAGAGCAC AAAGAACATG AAGCATTCAC GAGCCATTGA ACTGATCAAG 
AATGGAGGTC GGAGGGCAAG GCTTGTCCTG AAGAGAGGAG ATGGATCTGT ACCCGAGTAT 
GACGGCAGCA GTGACGGGTA CCCTTCCCCA TCCGGGGCAC AAAACGCTCC GGAAGTGAGA 
AAGCTGCCGC CCAACAGCCG ATATCACACC TCATTTGAGT CCAGTTATCC ACCAGACTTT 
CACAAACCAT CACGCCTGGA GGAGCCTTCG CGTGGCCGAA GTATGGAGAA GAACAGGGAC 
TGGGCCGGCT CAGATCTGAT GGACTTCCAT GGCCGGCAGT TTAACCCCCA CCTTCATCAC 
ACCTGGAATA ACAGTCACAG TCAAAGTATC CCCCGACAGC AGTCTCCCGA CAACAGTAAT 
GGCAGCACCG TTGGCAACAA GAAGAGCCGA GGACGCAAAG TCAAGAAGTC TGAATCAGAG 
AGTGGCATCT CCAAGCTCCT GAAAAGTCTA AGGAAAGACA GCTCAGGGAA GAAGGCTGCT 
GCGGCCGAAA AACTGCGAGG CAGAGAGCTC TCGAGCAGCA GTTCTACTCT GGACAGTAAA 
CTCCGTCCAC AGCGTCGTGG CTCCCTTGAT CGCTCACCAA CCAGGAAGCG TAACTCATCT 
CCGGATCGTC GGCGGGCAAA GTCCATGGAC CGCAGAGCAC ATCGGGACCA CACACCTGAG 
AGGGAATATG ATGACGGAGG GTTTCTCTCT GTAACACCTG AGAGAAAGCT CTATGAACGG 
AAGCTCCTCA AGGAGTCACC GTTAGCCGGT CGAGTCAGTG AGGTCTCCAC CCCTGAGCGA 
GTCAACAACA GTGCAGATTC TCTCACCAGA GGCCGGGCAT ACGATAAAGC CACTAAGAAC 
GGAAACCTTT TGAGAGACTC TTCCTCAGAA AGGAGGGAGG AGAGGCACAG AGGGAATGGG 
ACAATGGAGA GGCGGTACAG ACTGGAGCGG GACTCTTCCC CTGACAGCAA CTACAGTCGG 
GACGAGCTGA ACTATGCAGC AGCAGCAGCA CCCAGACTGA AGGACTACTA CAGCATGATG 
AAGAACAATG CTGCACACAA CAACGCTACC TACAACAAGA CAGGACACAA CAATACTGCT 
GGAAGACGCA GCTCAAACCA GATCCCTGAG CCCAAGAGAA GGCTGTACAA AGAAAACCCA 
AAAGACCTCA GTATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015239219.1
CDS	573..5390
Translation	MAKVIQKKNHWITKVNECTIIRDTSGELNVELHGGAENGEFTYIGDIREDAVVYQDGKLSKGELLLDVEGLSVSGLPLYDIYTVINCCKGPVRFKTVRQGNKLNKDLKHFLSLRFQKTSPDHELQKTIRANLYRHAVPCTTRPPRDGEVPGLDYNFLSVEDFLQLEDSGTLLEVGTYEGNYYGTPKPPRQPIIGTVTLSDAGSLPSTPKRTKSYNDMQKVRVVPADNDDDDQATEMNNSFTGNPNDQDESRSGILRPYPVRDNALPCGLNNAAISTAESGQQTLAEPQVSPEDPLGPLPENWEMAYTENGELYFIDTTKTLHQDMAVCFQSFHNTKTTSWLDPRCRDKVPRPLEECDDDEEGIHAEDLKSDLELPPGWERIDDPVYGVYYVDHINRKTQYENPVLEARHQKILEQQQPPQPPEGERYIREWIEEHSSAAAPLANYVPNHLETYRDPQVPPALPPGPVGVKRGKPFFTRNPAELKGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAAVDGKMETGDVIVSVNDTIVLGYTHAQVVKIFQSIPIGSMVDLALCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETFDVPANQAGPINGMREARSHSPSAEVASNGSHGYSADVVTLASAIATQPELITIHMEKGDKGFGFTIADSLTGGGQRVKQIVDYPRCRGLKEGDILMEVNKRNVQNMSHNQVVDLLSKCPRGSEVTMLVQRGVVPAKRSPKLVHQLERKDSQTSSQHSVCSHRSAHTDSPSHPPSAMPSEAVVPTPAAPTQPLPVLPPQDPADGTLTLQKKPDPFKIWAQSRSMYESRLPDCQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTPGYGVSKGEGDVPPSPASSHHSSTQAPSLTEGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGSAVVPASLQPYDVEIQRGENEGFGFVIVSSVSRPDAGTTLAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVSLRIIPGDESSNASLLTNAEKIATITTTHTPQTTELRNHSKSKGVPPPPPSQTHLQDAEYYSVDLERDSKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKNMKHSRAIELIKNGGRRARLVLKRGDGSVPEYDGSSDGYPSPSGAQNAPEVRKLPPNSRYHTSFESSYPPDFHKPSRLEEPSRGRSMEKNRDWAGSDLMDFHGRQFNPHLHHTWNNSHSQSIPRQQSPDNSNGSTVGNKKSRGRKVKKSESESGISKLLKSLRKDSSGKKAAAAEKLRGRELSSSSSTLDSKLRPQRRGSLDRSPTRKRNSSPDRRRAKSMDRRAHRDHTPEREYDDGGFLSVTPERKLYERKLLKESPLAGRVSEVSTPERVNNSADSLTRGRAYDKATKNGNLLRDSSSERREERHRGNGTMERRYRLERDSSPDSNYSRDELNYAAAAAPRLKDYYSMMKNNAAHNNATYNKTGHNNTAGRRSSNQIPEPKRRLYKENPKDLSI

Target ORF information:

RefSeq Version	XM_015383733.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X6, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015383733.1

ORF Insert Sequence:

ATGGCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACAA AAGTAAATGA GTGCACGATT 
ATCCGGGACA CGAGTGGGGA GTTGAACGTG GAGCTGCACG GTGGAGCGGA GAATGGAGAG 
TTTACATACA TTGGAGATAT CAGGGAGGAT GCTGTGGTTT ATCAGGACGG AAAGCTGTCC 
AAGGGAGAAC TACTGCTAGA CGTGGAGGGT CTGTCGGTGT CTGGATTACC CCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAGGGA CCTGTCAGGT TTAAAACTGT TCGACAAGGC 
AACAAGCTCA ACAAGGACCT AAAGCACTTC TTGAGCTTAA GGTTTCAGAA AACATCACCT 
GACCACGAGT TGCAGAAAAC CATCCGAGCC AATCTATATC GCCATGCTGT GCCTTGTACG 
ACCCGCCCCC CTCGAGACGG AGAGGTCCCA GGGTTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTGGAGGA CAGTGGCACA CTACTGGAAG TAGGAACATA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC TCCAAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
GCTGGCTCCC TTCCGTCCAC ACCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAAAGTC 
CGCGTAGTGC CTGCTGACAA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGTAACC CTAACGACCA GGACGAGAGC CGCAGCGGCA TCCTCCGGCC GTATCCCGTG 
CGTGATAATG CTCTACCCTG TGGTCTGAAC AATGCAGCCA TTTCCACTGC AGAGAGCGGG 
CAGCAGACTC TTGCAGAGCC CCAGGTCTCA CCAGAGGACC CACTGGGACC GCTGCCTGAA 
AACTGGGAGA TGGCCTACAC AGAGAACGGG GAACTGTACT TCATAGACAC GACAAAGACT 
CTCCACCAGG ACATGGCTGT GTGTTTTCAG AGCTTCCACA ACACAAAGAC CACTTCATGG 
CTGGATCCCC GGTGTAGAGA CAAAGTCCCC AGGCCTCTGG AAGAGTGTGA TGATGATGAA 
GAAGGGATAC ATGCGGAAGA CCTGAAAAGT GATCTAGAAC TGCCTCCAGG ATGGGAGAGA 
ATAGATGACC CAGTGTACGG AGTGTATTAT GTGGACCATA TAAACAGGAA GACCCAGTAT 
GAGAACCCAG TTTTGGAGGC GCGGCATCAA AAAATCTTGG AGCAGCAGCA ACCGCCGCAG 
CCTCCAGAAG GTGAGCGGTA TATCCGAGAG TGGATAGAGG AGCATTCGTC TGCTGCAGCT 
CCATTGGCAA ACTATGTTCC AAACCACCTT GAGACATACA GGGACCCACA AGTCCCACCA 
GCTCTACCTC CTGGCCCAGT CGGGGTTAAA AGGGGGAAGC CTTTCTTCAC CAGAAATCCT 
GCAGAACTGA AAGGAACCTT TATCAACACC AAGCTAAAGA AAAGTCGCCG TGGATTTGGC 
TTCACAGTCG TTGGTGGCGA TGAGCCTGAT GAATTCCTGC AGATCAAAAG TCTGGTGCTG 
GACGGGCCTG CAGCTGTCGA TGGCAAAATG GAGACAGGGG ATGTGATTGT CAGTGTAAAT 
GACACCATCG TTCTGGGCTA CACACATGCA CAAGTGGTGA AGATTTTCCA GTCCATCCCT 
ATCGGCTCAA TGGTGGACCT GGCCTTATGT CGAGGATATC CGCTGCCATT TGACCCAGAT 
GACCCAAACA CCAGCCTAGT GACCTCTGTG GCGATCTTGG ACAAGGAGCC TATCATTGTC 
AATGGACAAG AGACTTTTGA TGTACCCGCC AACCAGGCTG GACCCATCAA TGGCATGCGG 
GAGGCACGGT CGCACAGCCC CTCAGCGGAG GTGGCATCAA ACGGTTCACA TGGGTACTCC 
GCTGACGTGG TCACCCTGGC CTCAGCCATC GCCACCCAGC CAGAGCTGAT CACCATACAC 
ATGGAGAAGG GAGACAAAGG GTTTGGCTTC ACCATTGCTG ACAGCCTGAC AGGAGGCGGG 
CAGAGAGTCA AGCAGATAGT GGACTACCCA CGGTGCCGTG GCCTGAAAGA GGGGGACATT 
CTGATGGAGG TTAACAAGAG AAACGTCCAG AATATGAGCC ACAACCAGGT GGTTGATCTG 
CTCAGCAAAT GTCCGAGAGG CAGCGAAGTC ACCATGTTGG TGCAAAGAGG CGTGGTGCCA 
GCCAAGAGAA GCCCAAAGCT GGTGCACCAG TTGGAGAGAA AGGACAGTCA GACCAGCTCC 
CAGCACAGCG TTTGCAGCCA TCGAAGTGCT CACACCGACT CTCCAAGCCA CCCACCCTCT 
GCCATGCCAA GTGAGGCAGT GGTCCCCACT CCTGCTGCTC CCACACAGCC TCTGCCGGTC 
TTGCCCCCCC AAGACCCAGC AGATGGCACT CTCACCCTCC AGAAGAAGCC AGACCCATTC 
AAGATCTGGG CCCAGTCCAG AAGCATGTAT GAAAGTCGAT TGCCAGATTG CCAGGAGCAA 
GACATTTTCC TTTGGAGGAA GGATACTGGC TTTGGCTTCC GCATTTTAGG AGGAAATGAG 
CCTGGAGAAC CTATTTACAT TGGTCATATA GTGAAATATG GTGCTGCGGA TGAAGATGGC 
CGCCTGCGGT CTGGTGATGA GCTTATTTGT GTGGACGGCA CAGCGGTAGT GGGCAAGTCC 
CACCAGCTGG TGGTTCAGCT GATGCAGCAG GCTGCCAAAC AGGGTCATGT AAACCTCACC 
GTCAGGCGCA AGACCCCCGG ATATGGAGTG TCAAAGGGAG AGGGTGATGT TCCGCCATCG 
CCGGCCTCTT CCCATCACAG CAGCACTCAG GCGCCAAGCC TCACAGAAGG CAAGCGGACC 
CCTCAGGGAA GCCAAAACTC CCTGAATACT GTTAGCTCTG GCAGTGGATC TACCAGCGGT 
ATAGGCAGTG GTGGTGGAGG TGGCAGTGGA AGCGCTGTGG TGCCCGCCTC TCTCCAGCCA 
TACGACGTAG AGATCCAGCG GGGAGAAAAT GAGGGATTTG GTTTCGTCAT CGTATCATCC 
GTCTCCCGGC CTGATGCTGG AACCACATTG GCTGGAAATG CATGCGTTGC GATGCCCCAC 
AAAATAGGTC GTATCATAGA GGGCAGCCCG GCTGACCGCT GCGGGAAGCT GAAGGTTGGA 
GACCGGATTC TGGCTGTCAA TGGCTGCTCC ATCACAAACA AGTCACATTC GGACATCGTC 
AACCTGATCA AGGAGGCCGG GAACACTGTC TCACTCAGGA TCATTCCAGG CGATGAATCT 
TCCAATGCTT CTCTCCTGAC TAATGCAGAG AAAATCGCTA CCATCACCAC CACACACACA 
CCTCAAACCA CAGAGTTAAG GAATCATTCA AAGTCAAAAG GAGTTCCTCC TCCACCTCCT 
TCGCAAACTC ATCTGCAGGA TGCAGAATAC TATTCTGTGG ACCTTGAACG GGACAGTAAA 
GGCTTCGGCT TCAGCCTAAG AGGAGGACGG GAGTACAACA TGGACCTTTA TGTGCTGAGA 
CTAGCCGAGG ATGGGGCTGC TGTCAGAAAT GGCAAAATGA GGGTGGGAGA TGAGATCCTG 
GAGATTAATG GTGAGAGCAC AAAGAACATG AAGCATTCAC GAGCCATTGA ACTGATCAAG 
AATGGAGGTC GGAGGGCAAG GCTTGTCCTG AAGAGAGGAG ATGGATCTGT ACCCGAGTAT 
GACGGCAGCA GTGACGGGTA CCCTTCCCCA TCCGGGGCAC AAAACGCTCC GGAAGTGAGA 
AAGCTGCCGC CCAACAGCCG ATATCACACC TCATTTGAGT CCAGTTATCC ACCAGACTTT 
CACAAACCAT CACGCCTGGA GGAGCCTTCG CGTGGCCGAA GTATGGAGAA GAACAGGGAC 
TGGGCCGGCT CAGATCTGAT GGACTTCCAT GGCCGGCAGT TTAACCCCCA CCTTCATCAC 
ACCTGGAATA ACAGTCACAG TCAAAGTATC CCCCGACAGC AGTCTCCCGA CAACAGTAAT 
GGCAGCACCG TTGGCAACAA GAAGAGCCGA GGACGCAAAG TCAAGAAGTC TGAATCAGAG 
AGTGGCATCT CCAAGCTCCT GAAAAGTCTA AGGAAAGACA GCTCAGGGAA GAAGGCTGCT 
GCGGCCGAAA AACTGCGAGG CAGAGAGCTC TCGAGCAGCA GTTCTACTCT GGACAGTAAA 
CTCCGTCCAC AGCGTCGTGG CTCCCTTGAT CGCTCACCAA CCAGGAAGCG TAACTCATCT 
CCGGATCGTC GGCGGGCAAA GTCCATGGAC CGCAGAGCAC ATCGGGACCA CACACCTGAG 
AGGGAATATG ATGACGGAGG GTTTCTCTCT GTAACACCTG AGAGAAAGCT CTATGAACGG 
AAGCTCCTCA AGGAGTCACC GTTAGCCGGT CGAGTCAGTG AGGTCTCCAC CCCTGAGCGA 
GTCAACAACA GTGCAGATTC TCTCACCAGA GGCCGGGCAT ACGATAAAGC CACTAAGAAC 
GGAAACCTTT TGAGAGACTC TTCCTCAGAA AGGAGGGAGG AGAGGCACAG AGGGAATGGG 
ACAATGGAGA GGCGGTACAG ACTGGAGCGG GACTCTTCCC CTGACAGCAA CTACAGTCGG 
GACGAGCTGA ACTATGCAGC AGCAGCAGCA CCCAGACTGA AGGACTACTA CAGCATGATG 
AAGAACAATG CTGCACACAA CAACGCTACC TACAACAAGA CAGGACACAA CAATACTGCT 
GGAAGACGCA GCTCAAACCA GATCCCTGAG CCCAAGAGAA GGCTGTACAA AGAAAACCCA 
AAAGACCTCA GTATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107090390 ( XM_015383734.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015239220.1 Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X7, mRNA.

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CloneID	OCy14730
Clone ID Related Accession (Same CDS sequence)	XM_015383734.1
Accession Version	XM_015383734.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4815bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X7
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150746.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACAA AAGTAAATGA GTGCACGATT 
ATCCGGGACA CGAGTGGGGA GTTGAACGTG GAGCTGCACG GTGGAGCGGA GAATGGAGAG 
TTTACATACA TTGGAGATAT CAGGGAGGAT GCTGTGGTTT ATCAGGACGG AAAGCTGTCC 
AAGGGAGAAC TACTGCTAGA CGTGGAGGGT CTGTCGGTGT CTGGATTACC CCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAGGGA CCTGTCAGGT TTAAAACTGT TCGACAAGGC 
AACAAGCTCA ACAAGGACCT AAAGCACTTC TTGAGCTTAA GGTTTCAGAA AACATCACCT 
GACCACGAGT TGCAGAAAAC CATCCGAGCC AATCTATATC GCCATGCTGT GCCTTGTACG 
ACCCGCCCCC CTCGAGACGG AGAGGTCCCA GGGTTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTGGAGGA CAGTGGCACA CTACTGGAAG TAGGAACATA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC TCCAAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
GCTGGCTCCC TTCCGTCCAC ACCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAAAGTC 
CGCGTAGTGC CTGCTGACAA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGATCCT TAGTGCGTAG TCTCTTCCCC TCTCCTTTGC TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGCAG CGGCATCCTC CGGCCGTATC CCGTGCGTGA TAATGCTCTA 
CCCTGTGGTC TGAACAATGC AGCCATTTCC ACTGCAGAGA GCGGGCAGCA GACTCTTGCA 
GAGCCCCAGG TCTCACCAGA GGACCCACTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACAGAGA ACGGGGAACT GTACTTCATA GACCACAACA CAAAGACCAC TTCATGGCTG 
GATCCCCGGT GTAGAGACAA AGTCCCCAGG CCTCTGGAAG AGTGTGATGA TGATGAAGAA 
GGGATACATG CGGAAGACCT GAAAAGTGAT CTAGAACTGC CTCCAGGATG GGAGAGAATA 
GATGACCCAG TGTACGGAGT GTATTATGTG GACCATATAA ACAGGAAGAC CCAGTATGAG 
AACCCAGTTT TGGAGGCGCG GCATCAAAAA ATCTTGGAGC AGCAGCAACC GCCGCAGCCT 
CCAGAAGGTG AGCGGTATAT CCGAGAGTGG ATAGAGGAGC ATTCGTCTGC TGCAGCTCCA 
TTGGCAAACT ATGTTCCAAA CCACCTTGAG ACATACAGGG ACCCACAAGT CCCACCAGCT 
CTACCTCCTG GCCCAGTCGG GGTTAAAAGG GGGAAGCCTT TCTTCACCAG AAATCCTGCA 
GAACTGAAAG GAACCTTTAT CAACACCAAG CTAAAGAAAA GTCGCCGTGG ATTTGGCTTC 
ACAGTCGTTG GTGGCGATGA GCCTGATGAA TTCCTGCAGA TCAAAAGTCT GGTGCTGGAC 
GGGCCTGCAG CTGTCGATGG CAAAATGGAG ACAGGGGATG TGATTGTCAG TGTAAATGAC 
ACCATCGTTC TGGGCTACAC ACATGCACAA GTGGTGAAGA TTTTCCAGTC CATCCCTATC 
GGCTCAATGG TGGACCTGGC CTTATGTCGA GGATATCCGC TGCCATTTGA CCCAGATGAC 
CCAAACACCA GCCTAGTGAC CTCTGTGGCG ATCTTGGACA AGGAGCCTAT CATTGTCAAT 
GGACAAGAGA CTTTTGATGT ACCCGCCAAC CAGGCTGGAC CCATCAATGG CATGCGGGAG 
GCACGGTCGC ACAGCCCCTC AGCGGAGGTG GCATCAAACG GTTCACATGG GTACTCCGCT 
GACGTGGTCA CCCTGGCCTC AGCCATCGCC ACCCAGCCAG AGCTGATCAC CATACACATG 
GAGAAGGGAG ACAAAGGGTT TGGCTTCACC ATTGCTGACA GCCTGACAGG AGGCGGGCAG 
AGAGTCAAGC AGATAGTGGA CTACCCACGG TGCCGTGGCC TGAAAGAGGG GGACATTCTG 
ATGGAGGTTA ACAAGAGAAA CGTCCAGAAT ATGAGCCACA ACCAGGTGGT TGATCTGCTC 
AGCAAATGTC CGAGAGGCAG CGAAGTCACC ATGTTGGTGC AAAGAGGCGT GGTGCCAGCC 
AAGAGAAGCC CAAAGCTGGT GCACCAGTTG GAGAGAAAGG ACAGTCAGAC CAGCTCCCAG 
CACAGCGTTT GCAGCCATCG AAGTGCTCAC ACCGACTCTC CAAGCCACCC ACCCTCTGCC 
ATGCCAAGTG AGGCAGTGGT CCCCACTCCT GCTGCTCCCA CACAGCCTCT GCCGGTCTTG 
CCCCCCCAAG ACCCAGCAGA TGGCACTCTC ACCCTCCAGA AGAAGCCAGA CCCATTCAAG 
ATCTGGGCCC AGTCCAGAAG CATGTATGAA AGTCGATTGC CAGATTGCCA GGAGCAAGAC 
ATTTTCCTTT GGAGGAAGGA TACTGGCTTT GGCTTCCGCA TTTTAGGAGG AAATGAGCCT 
GGAGAACCTA TTTACATTGG TCATATAGTG AAATATGGTG CTGCGGATGA AGATGGCCGC 
CTGCGGTCTG GTGATGAGCT TATTTGTGTG GACGGCACAG CGGTAGTGGG CAAGTCCCAC 
CAGCTGGTGG TTCAGCTGAT GCAGCAGGCT GCCAAACAGG GTCATGTAAA CCTCACCGTC 
AGGCGCAAGA CCCCCGGATA TGGAGTGTCA AAGGGAGAGG GTGATGTTCC GCCATCGCCG 
GCCTCTTCCC ATCACAGCAG CACTCAGGCG CCAAGCCTCA CAGAAGGCAA GCGGACCCCT 
CAGGGAAGCC AAAACTCCCT GAATACTGTT AGCTCTGGCA GTGGATCTAC CAGCGGTATA 
GGCAGTGGTG GTGGAGGTGG CAGTGGAAGC GCTGTGGTGC CCGCCTCTCT CCAGCCATAC 
GACGTAGAGA TCCAGCGGGG AGAAAATGAG GGATTTGGTT TCGTCATCGT ATCATCCGTC 
TCCCGGCCTG ATGCTGGAAC CACATTGGCT GGAAATGCAT GCGTTGCGAT GCCCCACAAA 
ATAGGTCGTA TCATAGAGGG CAGCCCGGCT GACCGCTGCG GGAAGCTGAA GGTTGGAGAC 
CGGATTCTGG CTGTCAATGG CTGCTCCATC ACAAACAAGT CACATTCGGA CATCGTCAAC 
CTGATCAAGG AGGCCGGGAA CACTGTCTCA CTCAGGATCA TTCCAGGCGA TGAATCTTCC 
AATGCTTCTC TCCTGACTAA TGCAGAGAAA ATCGCTACCA TCACCACCAC ACACACACCT 
CAAACCACAG AGTTAAGGAA TCATTCAAAG TCAAAAGGAG TTCCTCCTCC ACCTCCTTCG 
CAAACTCATC TGCAGGATGC AGAATACTAT TCTGTGGACC TTGAACGGGA CAGTAAAGGC 
TTCGGCTTCA GCCTAAGAGG AGGACGGGAG TACAACATGG ACCTTTATGT GCTGAGACTA 
GCCGAGGATG GGGCTGCTGT CAGAAATGGC AAAATGAGGG TGGGAGATGA GATCCTGGAG 
ATTAATGGTG AGAGCACAAA GAACATGAAG CATTCACGAG CCATTGAACT GATCAAGAAT 
GGAGGTCGGA GGGCAAGGCT TGTCCTGAAG AGAGGAGATG GATCTGTACC CGAGTATGAC 
GGCAGCAGTG ACGGGTACCC TTCCCCATCC GGGGCACAAA ACGCTCCGGA AGTGAGAAAG 
CTGCCGCCCA ACAGCCGATA TCACACCTCA TTTGAGTCCA GTTATCCACC AGACTTTCAC 
AAACCATCAC GCCTGGAGGA GCCTTCGCGT GGCCGAAGTA TGGAGAAGAA CAGGGACTGG 
GCCGGCTCAG ATCTGATGGA CTTCCATGGC CGGCAGTTTA ACCCCCACCT TCATCACACC 
TGGAATAACA GTCACAGTCA AAGTATCCCC CGACAGCAGT CTCCCGACAA CAGTAATGGC 
AGCACCGTTG GCAACAAGAA GAGCCGAGGA CGCAAAGTCA AGAAGTCTGA ATCAGAGAGT 
GGCATCTCCA AGCTCCTGAA AAGTCTAAGG AAAGACAGCT CAGGGAAGAA GGCTGCTGCG 
GCCGAAAAAC TGCGAGGCAG AGAGCTCTCG AGCAGCAGTT CTACTCTGGA CAGTAAACTC 
CGTCCACAGC GTCGTGGCTC CCTTGATCGC TCACCAACCA GGAAGCGTAA CTCATCTCCG 
GATCGTCGGC GGGCAAAGTC CATGGACCGC AGAGCACATC GGGACCACAC ACCTGAGAGG 
GAATATGATG ACGGAGGGTT TCTCTCTGTA ACACCTGAGA GAAAGCTCTA TGAACGGAAG 
CTCCTCAAGG AGTCACCGTT AGCCGGTCGA GTCAGTGAGG TCTCCACCCC TGAGCGAGTC 
AACAACAGTG CAGATTCTCT CACCAGAGGC CGGGCATACG ATAAAGCCAC TAAGAACGGA 
AACCTTTTGA GAGACTCTTC CTCAGAAAGG AGGGAGGAGA GGCACAGAGG GAATGGGACA 
ATGGAGAGGC GGTACAGACT GGAGCGGGAC TCTTCCCCTG ACAGCAACTA CAGTCGGGAC 
GAGCTGAACT ATGCAGCAGC AGCAGCACCC AGACTGAAGG ACTACTACAG CATGATGAAG 
AACAATGCTG CACACAACAA CGCTACCTAC AACAAGACAG GACACAACAA TACTGCTGGA 
AGACGCAGCT CAAACCAGAT CCCTGAGCCC AAGAGAAGGC TGTACAAAGA AAACCCAAAA 
GACCTCAGTA TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015239220.1
CDS	574..5388
Translation	MAKVIQKKNHWITKVNECTIIRDTSGELNVELHGGAENGEFTYIGDIREDAVVYQDGKLSKGELLLDVEGLSVSGLPLYDIYTVINCCKGPVRFKTVRQGNKLNKDLKHFLSLRFQKTSPDHELQKTIRANLYRHAVPCTTRPPRDGEVPGLDYNFLSVEDFLQLEDSGTLLEVGTYEGNYYGTPKPPRQPIIGTVTLSDAGSLPSTPKRTKSYNDMQKVRVVPADNDDDDQATEMNNSFTGSLVRSLFPSPLLCTGNPNDQDESRSGILRPYPVRDNALPCGLNNAAISTAESGQQTLAEPQVSPEDPLGPLPENWEMAYTENGELYFIDHNTKTTSWLDPRCRDKVPRPLEECDDDEEGIHAEDLKSDLELPPGWERIDDPVYGVYYVDHINRKTQYENPVLEARHQKILEQQQPPQPPEGERYIREWIEEHSSAAAPLANYVPNHLETYRDPQVPPALPPGPVGVKRGKPFFTRNPAELKGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAAVDGKMETGDVIVSVNDTIVLGYTHAQVVKIFQSIPIGSMVDLALCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETFDVPANQAGPINGMREARSHSPSAEVASNGSHGYSADVVTLASAIATQPELITIHMEKGDKGFGFTIADSLTGGGQRVKQIVDYPRCRGLKEGDILMEVNKRNVQNMSHNQVVDLLSKCPRGSEVTMLVQRGVVPAKRSPKLVHQLERKDSQTSSQHSVCSHRSAHTDSPSHPPSAMPSEAVVPTPAAPTQPLPVLPPQDPADGTLTLQKKPDPFKIWAQSRSMYESRLPDCQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTPGYGVSKGEGDVPPSPASSHHSSTQAPSLTEGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGSAVVPASLQPYDVEIQRGENEGFGFVIVSSVSRPDAGTTLAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVSLRIIPGDESSNASLLTNAEKIATITTTHTPQTTELRNHSKSKGVPPPPPSQTHLQDAEYYSVDLERDSKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKNMKHSRAIELIKNGGRRARLVLKRGDGSVPEYDGSSDGYPSPSGAQNAPEVRKLPPNSRYHTSFESSYPPDFHKPSRLEEPSRGRSMEKNRDWAGSDLMDFHGRQFNPHLHHTWNNSHSQSIPRQQSPDNSNGSTVGNKKSRGRKVKKSESESGISKLLKSLRKDSSGKKAAAAEKLRGRELSSSSSTLDSKLRPQRRGSLDRSPTRKRNSSPDRRRAKSMDRRAHRDHTPEREYDDGGFLSVTPERKLYERKLLKESPLAGRVSEVSTPERVNNSADSLTRGRAYDKATKNGNLLRDSSSERREERHRGNGTMERRYRLERDSSPDSNYSRDELNYAAAAAPRLKDYYSMMKNNAAHNNATYNKTGHNNTAGRRSSNQIPEPKRRLYKENPKDLSI

Target ORF information:

RefSeq Version	XM_015383734.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X7, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015383734.1

ORF Insert Sequence:

ATGGCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACAA AAGTAAATGA GTGCACGATT 
ATCCGGGACA CGAGTGGGGA GTTGAACGTG GAGCTGCACG GTGGAGCGGA GAATGGAGAG 
TTTACATACA TTGGAGATAT CAGGGAGGAT GCTGTGGTTT ATCAGGACGG AAAGCTGTCC 
AAGGGAGAAC TACTGCTAGA CGTGGAGGGT CTGTCGGTGT CTGGATTACC CCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAGGGA CCTGTCAGGT TTAAAACTGT TCGACAAGGC 
AACAAGCTCA ACAAGGACCT AAAGCACTTC TTGAGCTTAA GGTTTCAGAA AACATCACCT 
GACCACGAGT TGCAGAAAAC CATCCGAGCC AATCTATATC GCCATGCTGT GCCTTGTACG 
ACCCGCCCCC CTCGAGACGG AGAGGTCCCA GGGTTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTGGAGGA CAGTGGCACA CTACTGGAAG TAGGAACATA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC TCCAAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
GCTGGCTCCC TTCCGTCCAC ACCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAAAGTC 
CGCGTAGTGC CTGCTGACAA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGATCCT TAGTGCGTAG TCTCTTCCCC TCTCCTTTGC TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGCAG CGGCATCCTC CGGCCGTATC CCGTGCGTGA TAATGCTCTA 
CCCTGTGGTC TGAACAATGC AGCCATTTCC ACTGCAGAGA GCGGGCAGCA GACTCTTGCA 
GAGCCCCAGG TCTCACCAGA GGACCCACTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACAGAGA ACGGGGAACT GTACTTCATA GACCACAACA CAAAGACCAC TTCATGGCTG 
GATCCCCGGT GTAGAGACAA AGTCCCCAGG CCTCTGGAAG AGTGTGATGA TGATGAAGAA 
GGGATACATG CGGAAGACCT GAAAAGTGAT CTAGAACTGC CTCCAGGATG GGAGAGAATA 
GATGACCCAG TGTACGGAGT GTATTATGTG GACCATATAA ACAGGAAGAC CCAGTATGAG 
AACCCAGTTT TGGAGGCGCG GCATCAAAAA ATCTTGGAGC AGCAGCAACC GCCGCAGCCT 
CCAGAAGGTG AGCGGTATAT CCGAGAGTGG ATAGAGGAGC ATTCGTCTGC TGCAGCTCCA 
TTGGCAAACT ATGTTCCAAA CCACCTTGAG ACATACAGGG ACCCACAAGT CCCACCAGCT 
CTACCTCCTG GCCCAGTCGG GGTTAAAAGG GGGAAGCCTT TCTTCACCAG AAATCCTGCA 
GAACTGAAAG GAACCTTTAT CAACACCAAG CTAAAGAAAA GTCGCCGTGG ATTTGGCTTC 
ACAGTCGTTG GTGGCGATGA GCCTGATGAA TTCCTGCAGA TCAAAAGTCT GGTGCTGGAC 
GGGCCTGCAG CTGTCGATGG CAAAATGGAG ACAGGGGATG TGATTGTCAG TGTAAATGAC 
ACCATCGTTC TGGGCTACAC ACATGCACAA GTGGTGAAGA TTTTCCAGTC CATCCCTATC 
GGCTCAATGG TGGACCTGGC CTTATGTCGA GGATATCCGC TGCCATTTGA CCCAGATGAC 
CCAAACACCA GCCTAGTGAC CTCTGTGGCG ATCTTGGACA AGGAGCCTAT CATTGTCAAT 
GGACAAGAGA CTTTTGATGT ACCCGCCAAC CAGGCTGGAC CCATCAATGG CATGCGGGAG 
GCACGGTCGC ACAGCCCCTC AGCGGAGGTG GCATCAAACG GTTCACATGG GTACTCCGCT 
GACGTGGTCA CCCTGGCCTC AGCCATCGCC ACCCAGCCAG AGCTGATCAC CATACACATG 
GAGAAGGGAG ACAAAGGGTT TGGCTTCACC ATTGCTGACA GCCTGACAGG AGGCGGGCAG 
AGAGTCAAGC AGATAGTGGA CTACCCACGG TGCCGTGGCC TGAAAGAGGG GGACATTCTG 
ATGGAGGTTA ACAAGAGAAA CGTCCAGAAT ATGAGCCACA ACCAGGTGGT TGATCTGCTC 
AGCAAATGTC CGAGAGGCAG CGAAGTCACC ATGTTGGTGC AAAGAGGCGT GGTGCCAGCC 
AAGAGAAGCC CAAAGCTGGT GCACCAGTTG GAGAGAAAGG ACAGTCAGAC CAGCTCCCAG 
CACAGCGTTT GCAGCCATCG AAGTGCTCAC ACCGACTCTC CAAGCCACCC ACCCTCTGCC 
ATGCCAAGTG AGGCAGTGGT CCCCACTCCT GCTGCTCCCA CACAGCCTCT GCCGGTCTTG 
CCCCCCCAAG ACCCAGCAGA TGGCACTCTC ACCCTCCAGA AGAAGCCAGA CCCATTCAAG 
ATCTGGGCCC AGTCCAGAAG CATGTATGAA AGTCGATTGC CAGATTGCCA GGAGCAAGAC 
ATTTTCCTTT GGAGGAAGGA TACTGGCTTT GGCTTCCGCA TTTTAGGAGG AAATGAGCCT 
GGAGAACCTA TTTACATTGG TCATATAGTG AAATATGGTG CTGCGGATGA AGATGGCCGC 
CTGCGGTCTG GTGATGAGCT TATTTGTGTG GACGGCACAG CGGTAGTGGG CAAGTCCCAC 
CAGCTGGTGG TTCAGCTGAT GCAGCAGGCT GCCAAACAGG GTCATGTAAA CCTCACCGTC 
AGGCGCAAGA CCCCCGGATA TGGAGTGTCA AAGGGAGAGG GTGATGTTCC GCCATCGCCG 
GCCTCTTCCC ATCACAGCAG CACTCAGGCG CCAAGCCTCA CAGAAGGCAA GCGGACCCCT 
CAGGGAAGCC AAAACTCCCT GAATACTGTT AGCTCTGGCA GTGGATCTAC CAGCGGTATA 
GGCAGTGGTG GTGGAGGTGG CAGTGGAAGC GCTGTGGTGC CCGCCTCTCT CCAGCCATAC 
GACGTAGAGA TCCAGCGGGG AGAAAATGAG GGATTTGGTT TCGTCATCGT ATCATCCGTC 
TCCCGGCCTG ATGCTGGAAC CACATTGGCT GGAAATGCAT GCGTTGCGAT GCCCCACAAA 
ATAGGTCGTA TCATAGAGGG CAGCCCGGCT GACCGCTGCG GGAAGCTGAA GGTTGGAGAC 
CGGATTCTGG CTGTCAATGG CTGCTCCATC ACAAACAAGT CACATTCGGA CATCGTCAAC 
CTGATCAAGG AGGCCGGGAA CACTGTCTCA CTCAGGATCA TTCCAGGCGA TGAATCTTCC 
AATGCTTCTC TCCTGACTAA TGCAGAGAAA ATCGCTACCA TCACCACCAC ACACACACCT 
CAAACCACAG AGTTAAGGAA TCATTCAAAG TCAAAAGGAG TTCCTCCTCC ACCTCCTTCG 
CAAACTCATC TGCAGGATGC AGAATACTAT TCTGTGGACC TTGAACGGGA CAGTAAAGGC 
TTCGGCTTCA GCCTAAGAGG AGGACGGGAG TACAACATGG ACCTTTATGT GCTGAGACTA 
GCCGAGGATG GGGCTGCTGT CAGAAATGGC AAAATGAGGG TGGGAGATGA GATCCTGGAG 
ATTAATGGTG AGAGCACAAA GAACATGAAG CATTCACGAG CCATTGAACT GATCAAGAAT 
GGAGGTCGGA GGGCAAGGCT TGTCCTGAAG AGAGGAGATG GATCTGTACC CGAGTATGAC 
GGCAGCAGTG ACGGGTACCC TTCCCCATCC GGGGCACAAA ACGCTCCGGA AGTGAGAAAG 
CTGCCGCCCA ACAGCCGATA TCACACCTCA TTTGAGTCCA GTTATCCACC AGACTTTCAC 
AAACCATCAC GCCTGGAGGA GCCTTCGCGT GGCCGAAGTA TGGAGAAGAA CAGGGACTGG 
GCCGGCTCAG ATCTGATGGA CTTCCATGGC CGGCAGTTTA ACCCCCACCT TCATCACACC 
TGGAATAACA GTCACAGTCA AAGTATCCCC CGACAGCAGT CTCCCGACAA CAGTAATGGC 
AGCACCGTTG GCAACAAGAA GAGCCGAGGA CGCAAAGTCA AGAAGTCTGA ATCAGAGAGT 
GGCATCTCCA AGCTCCTGAA AAGTCTAAGG AAAGACAGCT CAGGGAAGAA GGCTGCTGCG 
GCCGAAAAAC TGCGAGGCAG AGAGCTCTCG AGCAGCAGTT CTACTCTGGA CAGTAAACTC 
CGTCCACAGC GTCGTGGCTC CCTTGATCGC TCACCAACCA GGAAGCGTAA CTCATCTCCG 
GATCGTCGGC GGGCAAAGTC CATGGACCGC AGAGCACATC GGGACCACAC ACCTGAGAGG 
GAATATGATG ACGGAGGGTT TCTCTCTGTA ACACCTGAGA GAAAGCTCTA TGAACGGAAG 
CTCCTCAAGG AGTCACCGTT AGCCGGTCGA GTCAGTGAGG TCTCCACCCC TGAGCGAGTC 
AACAACAGTG CAGATTCTCT CACCAGAGGC CGGGCATACG ATAAAGCCAC TAAGAACGGA 
AACCTTTTGA GAGACTCTTC CTCAGAAAGG AGGGAGGAGA GGCACAGAGG GAATGGGACA 
ATGGAGAGGC GGTACAGACT GGAGCGGGAC TCTTCCCCTG ACAGCAACTA CAGTCGGGAC 
GAGCTGAACT ATGCAGCAGC AGCAGCACCC AGACTGAAGG ACTACTACAG CATGATGAAG 
AACAATGCTG CACACAACAA CGCTACCTAC AACAAGACAG GACACAACAA TACTGCTGGA 
AGACGCAGCT CAAACCAGAT CCCTGAGCCC AAGAGAAGGC TGTACAAAGA AAACCCAAAA 
GACCTCAGTA TCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107090390 ( XM_015383735.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015239221.1 Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X8, mRNA.

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CloneID	OCy14731
Clone ID Related Accession (Same CDS sequence)	XM_015383735.1
Accession Version	XM_015383735.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4812bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X8
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150746.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACAA AAGTAAATGA GTGCACGATT 
ATCCGGGACA CGAGTGGGGA GTTGAACGTG GAGCTGCACG GTGGAGCGGA GAATGGAGAG 
TTTACATACA TTGGAGATAT CAGGGAGGAT GCTGTGGTTT ATCAGGACGG AAAGCTGTCC 
AAGGGAGAAC TACTGCTAGA CGTGGAGGGT CTGTCGGTGT CTGGATTACC CCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAGGGA CCTGTCAGGT TTAAAACTGT TCGACAAGGC 
AACAAGCTCA ACAAGGACCT AAAGCACTTC TTGAGCTTAA GGTTTCAGAA AACATCACCT 
GACCACGAGT TGCAGAAAAC CATCCGAGCC AATCTATATC GCCATGCTGT GCCTTGTACG 
ACCCGCCCCC CTCGAGACGG AGAGGTCCCA GGGTTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTGGAGGA CAGTGGCACA CTACTGGAAG TAGGAACATA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC TCCAAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
GCTGGCTCCC TTCCGTCCAC ACCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAAAGTC 
CGCGTAGTGC CTGCTGACAA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGATCCT TAGTGCGTAG TCTCTTCCCC TCTCCTTTGC TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGCAG CGGCATCCTC CGGCCGTATC CCGTGCGTGA TAATGCTCTA 
CCCTGTGGTC TGAACAATGC AGCCATTTCC ACTGCAGAGA GCGGGCAGCA GACTCTTGCA 
GAGCCCCAGG TCTCACCAGA GGACCCACTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACAGAGA ACGGGGAACT GTACTTCATA GACCACAACA CAAAGACCAC TTCATGGCTG 
GATCCCCGGT GTAGAGACAA AGTCCCCAGG CCTCTGGAAG AGTGTGATGA TGATGAAGGG 
ATACATGCGG AAGACCTGAA AAGTGATCTA GAACTGCCTC CAGGATGGGA GAGAATAGAT 
GACCCAGTGT ACGGAGTGTA TTATGTGGAC CATATAAACA GGAAGACCCA GTATGAGAAC 
CCAGTTTTGG AGGCGCGGCA TCAAAAAATC TTGGAGCAGC AGCAACCGCC GCAGCCTCCA 
GAAGGTGAGC GGTATATCCG AGAGTGGATA GAGGAGCATT CGTCTGCTGC AGCTCCATTG 
GCAAACTATG TTCCAAACCA CCTTGAGACA TACAGGGACC CACAAGTCCC ACCAGCTCTA 
CCTCCTGGCC CAGTCGGGGT TAAAAGGGGG AAGCCTTTCT TCACCAGAAA TCCTGCAGAA 
CTGAAAGGAA CCTTTATCAA CACCAAGCTA AAGAAAAGTC GCCGTGGATT TGGCTTCACA 
GTCGTTGGTG GCGATGAGCC TGATGAATTC CTGCAGATCA AAAGTCTGGT GCTGGACGGG 
CCTGCAGCTG TCGATGGCAA AATGGAGACA GGGGATGTGA TTGTCAGTGT AAATGACACC 
ATCGTTCTGG GCTACACACA TGCACAAGTG GTGAAGATTT TCCAGTCCAT CCCTATCGGC 
TCAATGGTGG ACCTGGCCTT ATGTCGAGGA TATCCGCTGC CATTTGACCC AGATGACCCA 
AACACCAGCC TAGTGACCTC TGTGGCGATC TTGGACAAGG AGCCTATCAT TGTCAATGGA 
CAAGAGACTT TTGATGTACC CGCCAACCAG GCTGGACCCA TCAATGGCAT GCGGGAGGCA 
CGGTCGCACA GCCCCTCAGC GGAGGTGGCA TCAAACGGTT CACATGGGTA CTCCGCTGAC 
GTGGTCACCC TGGCCTCAGC CATCGCCACC CAGCCAGAGC TGATCACCAT ACACATGGAG 
AAGGGAGACA AAGGGTTTGG CTTCACCATT GCTGACAGCC TGACAGGAGG CGGGCAGAGA 
GTCAAGCAGA TAGTGGACTA CCCACGGTGC CGTGGCCTGA AAGAGGGGGA CATTCTGATG 
GAGGTTAACA AGAGAAACGT CCAGAATATG AGCCACAACC AGGTGGTTGA TCTGCTCAGC 
AAATGTCCGA GAGGCAGCGA AGTCACCATG TTGGTGCAAA GAGGCGTGGT GCCAGCCAAG 
AGAAGCCCAA AGCTGGTGCA CCAGTTGGAG AGAAAGGACA GTCAGACCAG CTCCCAGCAC 
AGCGTTTGCA GCCATCGAAG TGCTCACACC GACTCTCCAA GCCACCCACC CTCTGCCATG 
CCAAGTGAGG CAGTGGTCCC CACTCCTGCT GCTCCCACAC AGCCTCTGCC GGTCTTGCCC 
CCCCAAGACC CAGCAGATGG CACTCTCACC CTCCAGAAGA AGCCAGACCC ATTCAAGATC 
TGGGCCCAGT CCAGAAGCAT GTATGAAAGT CGATTGCCAG ATTGCCAGGA GCAAGACATT 
TTCCTTTGGA GGAAGGATAC TGGCTTTGGC TTCCGCATTT TAGGAGGAAA TGAGCCTGGA 
GAACCTATTT ACATTGGTCA TATAGTGAAA TATGGTGCTG CGGATGAAGA TGGCCGCCTG 
CGGTCTGGTG ATGAGCTTAT TTGTGTGGAC GGCACAGCGG TAGTGGGCAA GTCCCACCAG 
CTGGTGGTTC AGCTGATGCA GCAGGCTGCC AAACAGGGTC ATGTAAACCT CACCGTCAGG 
CGCAAGACCC CCGGATATGG AGTGTCAAAG GGAGAGGGTG ATGTTCCGCC ATCGCCGGCC 
TCTTCCCATC ACAGCAGCAC TCAGGCGCCA AGCCTCACAG AAGGCAAGCG GACCCCTCAG 
GGAAGCCAAA ACTCCCTGAA TACTGTTAGC TCTGGCAGTG GATCTACCAG CGGTATAGGC 
AGTGGTGGTG GAGGTGGCAG TGGAAGCGCT GTGGTGCCCG CCTCTCTCCA GCCATACGAC 
GTAGAGATCC AGCGGGGAGA AAATGAGGGA TTTGGTTTCG TCATCGTATC ATCCGTCTCC 
CGGCCTGATG CTGGAACCAC ATTGGCTGGA AATGCATGCG TTGCGATGCC CCACAAAATA 
GGTCGTATCA TAGAGGGCAG CCCGGCTGAC CGCTGCGGGA AGCTGAAGGT TGGAGACCGG 
ATTCTGGCTG TCAATGGCTG CTCCATCACA AACAAGTCAC ATTCGGACAT CGTCAACCTG 
ATCAAGGAGG CCGGGAACAC TGTCTCACTC AGGATCATTC CAGGCGATGA ATCTTCCAAT 
GCTTCTCTCC TGACTAATGC AGAGAAAATC GCTACCATCA CCACCACACA CACACCTCAA 
ACCACAGAGT TAAGGAATCA TTCAAAGTCA AAAGGAGTTC CTCCTCCACC TCCTTCGCAA 
ACTCATCTGC AGGATGCAGA ATACTATTCT GTGGACCTTG AACGGGACAG TAAAGGCTTC 
GGCTTCAGCC TAAGAGGAGG ACGGGAGTAC AACATGGACC TTTATGTGCT GAGACTAGCC 
GAGGATGGGG CTGCTGTCAG AAATGGCAAA ATGAGGGTGG GAGATGAGAT CCTGGAGATT 
AATGGTGAGA GCACAAAGAA CATGAAGCAT TCACGAGCCA TTGAACTGAT CAAGAATGGA 
GGTCGGAGGG CAAGGCTTGT CCTGAAGAGA GGAGATGGAT CTGTACCCGA GTATGACGGC 
AGCAGTGACG GGTACCCTTC CCCATCCGGG GCACAAAACG CTCCGGAAGT GAGAAAGCTG 
CCGCCCAACA GCCGATATCA CACCTCATTT GAGTCCAGTT ATCCACCAGA CTTTCACAAA 
CCATCACGCC TGGAGGAGCC TTCGCGTGGC CGAAGTATGG AGAAGAACAG GGACTGGGCC 
GGCTCAGATC TGATGGACTT CCATGGCCGG CAGTTTAACC CCCACCTTCA TCACACCTGG 
AATAACAGTC ACAGTCAAAG TATCCCCCGA CAGCAGTCTC CCGACAACAG TAATGGCAGC 
ACCGTTGGCA ACAAGAAGAG CCGAGGACGC AAAGTCAAGA AGTCTGAATC AGAGAGTGGC 
ATCTCCAAGC TCCTGAAAAG TCTAAGGAAA GACAGCTCAG GGAAGAAGGC TGCTGCGGCC 
GAAAAACTGC GAGGCAGAGA GCTCTCGAGC AGCAGTTCTA CTCTGGACAG TAAACTCCGT 
CCACAGCGTC GTGGCTCCCT TGATCGCTCA CCAACCAGGA AGCGTAACTC ATCTCCGGAT 
CGTCGGCGGG CAAAGTCCAT GGACCGCAGA GCACATCGGG ACCACACACC TGAGAGGGAA 
TATGATGACG GAGGGTTTCT CTCTGTAACA CCTGAGAGAA AGCTCTATGA ACGGAAGCTC 
CTCAAGGAGT CACCGTTAGC CGGTCGAGTC AGTGAGGTCT CCACCCCTGA GCGAGTCAAC 
AACAGTGCAG ATTCTCTCAC CAGAGGCCGG GCATACGATA AAGCCACTAA GAACGGAAAC 
CTTTTGAGAG ACTCTTCCTC AGAAAGGAGG GAGGAGAGGC ACAGAGGGAA TGGGACAATG 
GAGAGGCGGT ACAGACTGGA GCGGGACTCT TCCCCTGACA GCAACTACAG TCGGGACGAG 
CTGAACTATG CAGCAGCAGC AGCACCCAGA CTGAAGGACT ACTACAGCAT GATGAAGAAC 
AATGCTGCAC ACAACAACGC TACCTACAAC AAGACAGGAC ACAACAATAC TGCTGGAAGA 
CGCAGCTCAA ACCAGATCCC TGAGCCCAAG AGAAGGCTGT ACAAAGAAAA CCCAAAAGAC 
CTCAGTATCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015239221.1
CDS	574..5385
Translation	MAKVIQKKNHWITKVNECTIIRDTSGELNVELHGGAENGEFTYIGDIREDAVVYQDGKLSKGELLLDVEGLSVSGLPLYDIYTVINCCKGPVRFKTVRQGNKLNKDLKHFLSLRFQKTSPDHELQKTIRANLYRHAVPCTTRPPRDGEVPGLDYNFLSVEDFLQLEDSGTLLEVGTYEGNYYGTPKPPRQPIIGTVTLSDAGSLPSTPKRTKSYNDMQKVRVVPADNDDDDQATEMNNSFTGSLVRSLFPSPLLCTGNPNDQDESRSGILRPYPVRDNALPCGLNNAAISTAESGQQTLAEPQVSPEDPLGPLPENWEMAYTENGELYFIDHNTKTTSWLDPRCRDKVPRPLEECDDDEGIHAEDLKSDLELPPGWERIDDPVYGVYYVDHINRKTQYENPVLEARHQKILEQQQPPQPPEGERYIREWIEEHSSAAAPLANYVPNHLETYRDPQVPPALPPGPVGVKRGKPFFTRNPAELKGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAAVDGKMETGDVIVSVNDTIVLGYTHAQVVKIFQSIPIGSMVDLALCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETFDVPANQAGPINGMREARSHSPSAEVASNGSHGYSADVVTLASAIATQPELITIHMEKGDKGFGFTIADSLTGGGQRVKQIVDYPRCRGLKEGDILMEVNKRNVQNMSHNQVVDLLSKCPRGSEVTMLVQRGVVPAKRSPKLVHQLERKDSQTSSQHSVCSHRSAHTDSPSHPPSAMPSEAVVPTPAAPTQPLPVLPPQDPADGTLTLQKKPDPFKIWAQSRSMYESRLPDCQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTPGYGVSKGEGDVPPSPASSHHSSTQAPSLTEGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGSAVVPASLQPYDVEIQRGENEGFGFVIVSSVSRPDAGTTLAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVSLRIIPGDESSNASLLTNAEKIATITTTHTPQTTELRNHSKSKGVPPPPPSQTHLQDAEYYSVDLERDSKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKNMKHSRAIELIKNGGRRARLVLKRGDGSVPEYDGSSDGYPSPSGAQNAPEVRKLPPNSRYHTSFESSYPPDFHKPSRLEEPSRGRSMEKNRDWAGSDLMDFHGRQFNPHLHHTWNNSHSQSIPRQQSPDNSNGSTVGNKKSRGRKVKKSESESGISKLLKSLRKDSSGKKAAAAEKLRGRELSSSSSTLDSKLRPQRRGSLDRSPTRKRNSSPDRRRAKSMDRRAHRDHTPEREYDDGGFLSVTPERKLYERKLLKESPLAGRVSEVSTPERVNNSADSLTRGRAYDKATKNGNLLRDSSSERREERHRGNGTMERRYRLERDSSPDSNYSRDELNYAAAAAPRLKDYYSMMKNNAAHNNATYNKTGHNNTAGRRSSNQIPEPKRRLYKENPKDLSI

Target ORF information:

RefSeq Version	XM_015383735.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X8, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015383735.1

ORF Insert Sequence:

ATGGCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACAA AAGTAAATGA GTGCACGATT 
ATCCGGGACA CGAGTGGGGA GTTGAACGTG GAGCTGCACG GTGGAGCGGA GAATGGAGAG 
TTTACATACA TTGGAGATAT CAGGGAGGAT GCTGTGGTTT ATCAGGACGG AAAGCTGTCC 
AAGGGAGAAC TACTGCTAGA CGTGGAGGGT CTGTCGGTGT CTGGATTACC CCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAGGGA CCTGTCAGGT TTAAAACTGT TCGACAAGGC 
AACAAGCTCA ACAAGGACCT AAAGCACTTC TTGAGCTTAA GGTTTCAGAA AACATCACCT 
GACCACGAGT TGCAGAAAAC CATCCGAGCC AATCTATATC GCCATGCTGT GCCTTGTACG 
ACCCGCCCCC CTCGAGACGG AGAGGTCCCA GGGTTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTGGAGGA CAGTGGCACA CTACTGGAAG TAGGAACATA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC TCCAAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
GCTGGCTCCC TTCCGTCCAC ACCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAAAGTC 
CGCGTAGTGC CTGCTGACAA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGATCCT TAGTGCGTAG TCTCTTCCCC TCTCCTTTGC TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGCAG CGGCATCCTC CGGCCGTATC CCGTGCGTGA TAATGCTCTA 
CCCTGTGGTC TGAACAATGC AGCCATTTCC ACTGCAGAGA GCGGGCAGCA GACTCTTGCA 
GAGCCCCAGG TCTCACCAGA GGACCCACTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACAGAGA ACGGGGAACT GTACTTCATA GACCACAACA CAAAGACCAC TTCATGGCTG 
GATCCCCGGT GTAGAGACAA AGTCCCCAGG CCTCTGGAAG AGTGTGATGA TGATGAAGGG 
ATACATGCGG AAGACCTGAA AAGTGATCTA GAACTGCCTC CAGGATGGGA GAGAATAGAT 
GACCCAGTGT ACGGAGTGTA TTATGTGGAC CATATAAACA GGAAGACCCA GTATGAGAAC 
CCAGTTTTGG AGGCGCGGCA TCAAAAAATC TTGGAGCAGC AGCAACCGCC GCAGCCTCCA 
GAAGGTGAGC GGTATATCCG AGAGTGGATA GAGGAGCATT CGTCTGCTGC AGCTCCATTG 
GCAAACTATG TTCCAAACCA CCTTGAGACA TACAGGGACC CACAAGTCCC ACCAGCTCTA 
CCTCCTGGCC CAGTCGGGGT TAAAAGGGGG AAGCCTTTCT TCACCAGAAA TCCTGCAGAA 
CTGAAAGGAA CCTTTATCAA CACCAAGCTA AAGAAAAGTC GCCGTGGATT TGGCTTCACA 
GTCGTTGGTG GCGATGAGCC TGATGAATTC CTGCAGATCA AAAGTCTGGT GCTGGACGGG 
CCTGCAGCTG TCGATGGCAA AATGGAGACA GGGGATGTGA TTGTCAGTGT AAATGACACC 
ATCGTTCTGG GCTACACACA TGCACAAGTG GTGAAGATTT TCCAGTCCAT CCCTATCGGC 
TCAATGGTGG ACCTGGCCTT ATGTCGAGGA TATCCGCTGC CATTTGACCC AGATGACCCA 
AACACCAGCC TAGTGACCTC TGTGGCGATC TTGGACAAGG AGCCTATCAT TGTCAATGGA 
CAAGAGACTT TTGATGTACC CGCCAACCAG GCTGGACCCA TCAATGGCAT GCGGGAGGCA 
CGGTCGCACA GCCCCTCAGC GGAGGTGGCA TCAAACGGTT CACATGGGTA CTCCGCTGAC 
GTGGTCACCC TGGCCTCAGC CATCGCCACC CAGCCAGAGC TGATCACCAT ACACATGGAG 
AAGGGAGACA AAGGGTTTGG CTTCACCATT GCTGACAGCC TGACAGGAGG CGGGCAGAGA 
GTCAAGCAGA TAGTGGACTA CCCACGGTGC CGTGGCCTGA AAGAGGGGGA CATTCTGATG 
GAGGTTAACA AGAGAAACGT CCAGAATATG AGCCACAACC AGGTGGTTGA TCTGCTCAGC 
AAATGTCCGA GAGGCAGCGA AGTCACCATG TTGGTGCAAA GAGGCGTGGT GCCAGCCAAG 
AGAAGCCCAA AGCTGGTGCA CCAGTTGGAG AGAAAGGACA GTCAGACCAG CTCCCAGCAC 
AGCGTTTGCA GCCATCGAAG TGCTCACACC GACTCTCCAA GCCACCCACC CTCTGCCATG 
CCAAGTGAGG CAGTGGTCCC CACTCCTGCT GCTCCCACAC AGCCTCTGCC GGTCTTGCCC 
CCCCAAGACC CAGCAGATGG CACTCTCACC CTCCAGAAGA AGCCAGACCC ATTCAAGATC 
TGGGCCCAGT CCAGAAGCAT GTATGAAAGT CGATTGCCAG ATTGCCAGGA GCAAGACATT 
TTCCTTTGGA GGAAGGATAC TGGCTTTGGC TTCCGCATTT TAGGAGGAAA TGAGCCTGGA 
GAACCTATTT ACATTGGTCA TATAGTGAAA TATGGTGCTG CGGATGAAGA TGGCCGCCTG 
CGGTCTGGTG ATGAGCTTAT TTGTGTGGAC GGCACAGCGG TAGTGGGCAA GTCCCACCAG 
CTGGTGGTTC AGCTGATGCA GCAGGCTGCC AAACAGGGTC ATGTAAACCT CACCGTCAGG 
CGCAAGACCC CCGGATATGG AGTGTCAAAG GGAGAGGGTG ATGTTCCGCC ATCGCCGGCC 
TCTTCCCATC ACAGCAGCAC TCAGGCGCCA AGCCTCACAG AAGGCAAGCG GACCCCTCAG 
GGAAGCCAAA ACTCCCTGAA TACTGTTAGC TCTGGCAGTG GATCTACCAG CGGTATAGGC 
AGTGGTGGTG GAGGTGGCAG TGGAAGCGCT GTGGTGCCCG CCTCTCTCCA GCCATACGAC 
GTAGAGATCC AGCGGGGAGA AAATGAGGGA TTTGGTTTCG TCATCGTATC ATCCGTCTCC 
CGGCCTGATG CTGGAACCAC ATTGGCTGGA AATGCATGCG TTGCGATGCC CCACAAAATA 
GGTCGTATCA TAGAGGGCAG CCCGGCTGAC CGCTGCGGGA AGCTGAAGGT TGGAGACCGG 
ATTCTGGCTG TCAATGGCTG CTCCATCACA AACAAGTCAC ATTCGGACAT CGTCAACCTG 
ATCAAGGAGG CCGGGAACAC TGTCTCACTC AGGATCATTC CAGGCGATGA ATCTTCCAAT 
GCTTCTCTCC TGACTAATGC AGAGAAAATC GCTACCATCA CCACCACACA CACACCTCAA 
ACCACAGAGT TAAGGAATCA TTCAAAGTCA AAAGGAGTTC CTCCTCCACC TCCTTCGCAA 
ACTCATCTGC AGGATGCAGA ATACTATTCT GTGGACCTTG AACGGGACAG TAAAGGCTTC 
GGCTTCAGCC TAAGAGGAGG ACGGGAGTAC AACATGGACC TTTATGTGCT GAGACTAGCC 
GAGGATGGGG CTGCTGTCAG AAATGGCAAA ATGAGGGTGG GAGATGAGAT CCTGGAGATT 
AATGGTGAGA GCACAAAGAA CATGAAGCAT TCACGAGCCA TTGAACTGAT CAAGAATGGA 
GGTCGGAGGG CAAGGCTTGT CCTGAAGAGA GGAGATGGAT CTGTACCCGA GTATGACGGC 
AGCAGTGACG GGTACCCTTC CCCATCCGGG GCACAAAACG CTCCGGAAGT GAGAAAGCTG 
CCGCCCAACA GCCGATATCA CACCTCATTT GAGTCCAGTT ATCCACCAGA CTTTCACAAA 
CCATCACGCC TGGAGGAGCC TTCGCGTGGC CGAAGTATGG AGAAGAACAG GGACTGGGCC 
GGCTCAGATC TGATGGACTT CCATGGCCGG CAGTTTAACC CCCACCTTCA TCACACCTGG 
AATAACAGTC ACAGTCAAAG TATCCCCCGA CAGCAGTCTC CCGACAACAG TAATGGCAGC 
ACCGTTGGCA ACAAGAAGAG CCGAGGACGC AAAGTCAAGA AGTCTGAATC AGAGAGTGGC 
ATCTCCAAGC TCCTGAAAAG TCTAAGGAAA GACAGCTCAG GGAAGAAGGC TGCTGCGGCC 
GAAAAACTGC GAGGCAGAGA GCTCTCGAGC AGCAGTTCTA CTCTGGACAG TAAACTCCGT 
CCACAGCGTC GTGGCTCCCT TGATCGCTCA CCAACCAGGA AGCGTAACTC ATCTCCGGAT 
CGTCGGCGGG CAAAGTCCAT GGACCGCAGA GCACATCGGG ACCACACACC TGAGAGGGAA 
TATGATGACG GAGGGTTTCT CTCTGTAACA CCTGAGAGAA AGCTCTATGA ACGGAAGCTC 
CTCAAGGAGT CACCGTTAGC CGGTCGAGTC AGTGAGGTCT CCACCCCTGA GCGAGTCAAC 
AACAGTGCAG ATTCTCTCAC CAGAGGCCGG GCATACGATA AAGCCACTAA GAACGGAAAC 
CTTTTGAGAG ACTCTTCCTC AGAAAGGAGG GAGGAGAGGC ACAGAGGGAA TGGGACAATG 
GAGAGGCGGT ACAGACTGGA GCGGGACTCT TCCCCTGACA GCAACTACAG TCGGGACGAG 
CTGAACTATG CAGCAGCAGC AGCACCCAGA CTGAAGGACT ACTACAGCAT GATGAAGAAC 
AATGCTGCAC ACAACAACGC TACCTACAAC AAGACAGGAC ACAACAATAC TGCTGGAAGA 
CGCAGCTCAA ACCAGATCCC TGAGCCCAAG AGAAGGCTGT ACAAAGAAAA CCCAAAAGAC 
CTCAGTATCT AG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107090390 ( XM_015383736.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015239222.1 Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X9, mRNA.

Price & Availability↑

CloneID	OCy14732
Clone ID Related Accession (Same CDS sequence)	XM_015383736.1
Accession Version	XM_015383736.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4776bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X9
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150746.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACAA AAGTAAATGA GTGCACGATT 
ATCCGGGACA CGAGTGGGGA GTTGAACGTG GAGCTGCACG GTGGAGCGGA GAATGGAGAG 
TTTACATACA TTGGAGATAT CAGGGAGGAT GCTGTGGTTT ATCAGGACGG AAAGCTGTCC 
AAGGGAGAAC TACTGCTAGA CGTGGAGGGT CTGTCGGTGT CTGGATTACC CCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAGGGA CCTGTCAGGT TTAAAACTGT TCGACAAGGC 
AACAAGCTCA ACAAGGACCT AAAGCACTTC TTGAGCTTAA GGTTTCAGAA AACATCACCT 
GACCACGAGT TGCAGAAAAC CATCCGAGCC AATCTATATC GCCATGCTGT GCCTTGTACG 
ACCCGCCCCC CTCGAGACGG AGAGGTCCCA GGGTTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTGGAGGA CAGTGGCACA CTACTGGAAG TAGGAACATA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC TCCAAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
GCTGGCTCCC TTCCGTCCAC ACCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAAAGTC 
CGCGTAGTGC CTGCTGACAA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGATCCT TAGTGCGTAG TCTCTTCCCC TCTCCTTTGC TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGCAG CGGCATCCTC CGGCCGTATC CCGTGCGTGA TAATGCTCTA 
CCCTGTGGTC TGAACAATGC AGCCATTTCC ACTGCAGAGA GCGGGCAGCA GACTCTTGCA 
GAGCCCCAGG TCTCACCAGA GGACCCACTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACAGAGA ACGGGGAACT GTACTTCATA GACCACAACA CAAAGACCAC TTCATGGCTG 
GATCCCCGGT GTAGAGACAA AGTCCCCAGG CCTCTGGAAG AGTGTGATGA TGATGAACTG 
CCTCCAGGAT GGGAGAGAAT AGATGACCCA GTGTACGGAG TGTATTATGT GGACCATATA 
AACAGGAAGA CCCAGTATGA GAACCCAGTT TTGGAGGCGC GGCATCAAAA AATCTTGGAG 
CAGCAGCAAC CGCCGCAGCC TCCAGAAGGT GAGCGGTATA TCCGAGAGTG GATAGAGGAG 
CATTCGTCTG CTGCAGCTCC ATTGGCAAAC TATGTTCCAA ACCACCTTGA GACATACAGG 
GACCCACAAG TCCCACCAGC TCTACCTCCT GGCCCAGTCG GGGTTAAAAG GGGGAAGCCT 
TTCTTCACCA GAAATCCTGC AGAACTGAAA GGAACCTTTA TCAACACCAA GCTAAAGAAA 
AGTCGCCGTG GATTTGGCTT CACAGTCGTT GGTGGCGATG AGCCTGATGA ATTCCTGCAG 
ATCAAAAGTC TGGTGCTGGA CGGGCCTGCA GCTGTCGATG GCAAAATGGA GACAGGGGAT 
GTGATTGTCA GTGTAAATGA CACCATCGTT CTGGGCTACA CACATGCACA AGTGGTGAAG 
ATTTTCCAGT CCATCCCTAT CGGCTCAATG GTGGACCTGG CCTTATGTCG AGGATATCCG 
CTGCCATTTG ACCCAGATGA CCCAAACACC AGCCTAGTGA CCTCTGTGGC GATCTTGGAC 
AAGGAGCCTA TCATTGTCAA TGGACAAGAG ACTTTTGATG TACCCGCCAA CCAGGCTGGA 
CCCATCAATG GCATGCGGGA GGCACGGTCG CACAGCCCCT CAGCGGAGGT GGCATCAAAC 
GGTTCACATG GGTACTCCGC TGACGTGGTC ACCCTGGCCT CAGCCATCGC CACCCAGCCA 
GAGCTGATCA CCATACACAT GGAGAAGGGA GACAAAGGGT TTGGCTTCAC CATTGCTGAC 
AGCCTGACAG GAGGCGGGCA GAGAGTCAAG CAGATAGTGG ACTACCCACG GTGCCGTGGC 
CTGAAAGAGG GGGACATTCT GATGGAGGTT AACAAGAGAA ACGTCCAGAA TATGAGCCAC 
AACCAGGTGG TTGATCTGCT CAGCAAATGT CCGAGAGGCA GCGAAGTCAC CATGTTGGTG 
CAAAGAGGCG TGGTGCCAGC CAAGAGAAGC CCAAAGCTGG TGCACCAGTT GGAGAGAAAG 
GACAGTCAGA CCAGCTCCCA GCACAGCGTT TGCAGCCATC GAAGTGCTCA CACCGACTCT 
CCAAGCCACC CACCCTCTGC CATGCCAAGT GAGGCAGTGG TCCCCACTCC TGCTGCTCCC 
ACACAGCCTC TGCCGGTCTT GCCCCCCCAA GACCCAGCAG ATGGCACTCT CACCCTCCAG 
AAGAAGCCAG ACCCATTCAA GATCTGGGCC CAGTCCAGAA GCATGTATGA AAGTCGATTG 
CCAGATTGCC AGGAGCAAGA CATTTTCCTT TGGAGGAAGG ATACTGGCTT TGGCTTCCGC 
ATTTTAGGAG GAAATGAGCC TGGAGAACCT ATTTACATTG GTCATATAGT GAAATATGGT 
GCTGCGGATG AAGATGGCCG CCTGCGGTCT GGTGATGAGC TTATTTGTGT GGACGGCACA 
GCGGTAGTGG GCAAGTCCCA CCAGCTGGTG GTTCAGCTGA TGCAGCAGGC TGCCAAACAG 
GGTCATGTAA ACCTCACCGT CAGGCGCAAG ACCCCCGGAT ATGGAGTGTC AAAGGGAGAG 
GGTGATGTTC CGCCATCGCC GGCCTCTTCC CATCACAGCA GCACTCAGGC GCCAAGCCTC 
ACAGAAGGCA AGCGGACCCC TCAGGGAAGC CAAAACTCCC TGAATACTGT TAGCTCTGGC 
AGTGGATCTA CCAGCGGTAT AGGCAGTGGT GGTGGAGGTG GCAGTGGAAG CGCTGTGGTG 
CCCGCCTCTC TCCAGCCATA CGACGTAGAG ATCCAGCGGG GAGAAAATGA GGGATTTGGT 
TTCGTCATCG TATCATCCGT CTCCCGGCCT GATGCTGGAA CCACATTGGC TGGAAATGCA 
TGCGTTGCGA TGCCCCACAA AATAGGTCGT ATCATAGAGG GCAGCCCGGC TGACCGCTGC 
GGGAAGCTGA AGGTTGGAGA CCGGATTCTG GCTGTCAATG GCTGCTCCAT CACAAACAAG 
TCACATTCGG ACATCGTCAA CCTGATCAAG GAGGCCGGGA ACACTGTCTC ACTCAGGATC 
ATTCCAGGCG ATGAATCTTC CAATGCTTCT CTCCTGACTA ATGCAGAGAA AATCGCTACC 
ATCACCACCA CACACACACC TCAAACCACA GAGTTAAGGA ATCATTCAAA GTCAAAAGGA 
GTTCCTCCTC CACCTCCTTC GCAAACTCAT CTGCAGGATG CAGAATACTA TTCTGTGGAC 
CTTGAACGGG ACAGTAAAGG CTTCGGCTTC AGCCTAAGAG GAGGACGGGA GTACAACATG 
GACCTTTATG TGCTGAGACT AGCCGAGGAT GGGGCTGCTG TCAGAAATGG CAAAATGAGG 
GTGGGAGATG AGATCCTGGA GATTAATGGT GAGAGCACAA AGAACATGAA GCATTCACGA 
GCCATTGAAC TGATCAAGAA TGGAGGTCGG AGGGCAAGGC TTGTCCTGAA GAGAGGAGAT 
GGATCTGTAC CCGAGTATGA CGGCAGCAGT GACGGGTACC CTTCCCCATC CGGGGCACAA 
AACGCTCCGG AAGTGAGAAA GCTGCCGCCC AACAGCCGAT ATCACACCTC ATTTGAGTCC 
AGTTATCCAC CAGACTTTCA CAAACCATCA CGCCTGGAGG AGCCTTCGCG TGGCCGAAGT 
ATGGAGAAGA ACAGGGACTG GGCCGGCTCA GATCTGATGG ACTTCCATGG CCGGCAGTTT 
AACCCCCACC TTCATCACAC CTGGAATAAC AGTCACAGTC AAAGTATCCC CCGACAGCAG 
TCTCCCGACA ACAGTAATGG CAGCACCGTT GGCAACAAGA AGAGCCGAGG ACGCAAAGTC 
AAGAAGTCTG AATCAGAGAG TGGCATCTCC AAGCTCCTGA AAAGTCTAAG GAAAGACAGC 
TCAGGGAAGA AGGCTGCTGC GGCCGAAAAA CTGCGAGGCA GAGAGCTCTC GAGCAGCAGT 
TCTACTCTGG ACAGTAAACT CCGTCCACAG CGTCGTGGCT CCCTTGATCG CTCACCAACC 
AGGAAGCGTA ACTCATCTCC GGATCGTCGG CGGGCAAAGT CCATGGACCG CAGAGCACAT 
CGGGACCACA CACCTGAGAG GGAATATGAT GACGGAGGGT TTCTCTCTGT AACACCTGAG 
AGAAAGCTCT ATGAACGGAA GCTCCTCAAG GAGTCACCGT TAGCCGGTCG AGTCAGTGAG 
GTCTCCACCC CTGAGCGAGT CAACAACAGT GCAGATTCTC TCACCAGAGG CCGGGCATAC 
GATAAAGCCA CTAAGAACGG AAACCTTTTG AGAGACTCTT CCTCAGAAAG GAGGGAGGAG 
AGGCACAGAG GGAATGGGAC AATGGAGAGG CGGTACAGAC TGGAGCGGGA CTCTTCCCCT 
GACAGCAACT ACAGTCGGGA CGAGCTGAAC TATGCAGCAG CAGCAGCACC CAGACTGAAG 
GACTACTACA GCATGATGAA GAACAATGCT GCACACAACA ACGCTACCTA CAACAAGACA 
GGACACAACA ATACTGCTGG AAGACGCAGC TCAAACCAGA TCCCTGAGCC CAAGAGAAGG 
CTGTACAAAG AAAACCCAAA AGACCTCAGT ATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015239222.1
CDS	575..5350
Translation	MAKVIQKKNHWITKVNECTIIRDTSGELNVELHGGAENGEFTYIGDIREDAVVYQDGKLSKGELLLDVEGLSVSGLPLYDIYTVINCCKGPVRFKTVRQGNKLNKDLKHFLSLRFQKTSPDHELQKTIRANLYRHAVPCTTRPPRDGEVPGLDYNFLSVEDFLQLEDSGTLLEVGTYEGNYYGTPKPPRQPIIGTVTLSDAGSLPSTPKRTKSYNDMQKVRVVPADNDDDDQATEMNNSFTGSLVRSLFPSPLLCTGNPNDQDESRSGILRPYPVRDNALPCGLNNAAISTAESGQQTLAEPQVSPEDPLGPLPENWEMAYTENGELYFIDHNTKTTSWLDPRCRDKVPRPLEECDDDELPPGWERIDDPVYGVYYVDHINRKTQYENPVLEARHQKILEQQQPPQPPEGERYIREWIEEHSSAAAPLANYVPNHLETYRDPQVPPALPPGPVGVKRGKPFFTRNPAELKGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAAVDGKMETGDVIVSVNDTIVLGYTHAQVVKIFQSIPIGSMVDLALCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETFDVPANQAGPINGMREARSHSPSAEVASNGSHGYSADVVTLASAIATQPELITIHMEKGDKGFGFTIADSLTGGGQRVKQIVDYPRCRGLKEGDILMEVNKRNVQNMSHNQVVDLLSKCPRGSEVTMLVQRGVVPAKRSPKLVHQLERKDSQTSSQHSVCSHRSAHTDSPSHPPSAMPSEAVVPTPAAPTQPLPVLPPQDPADGTLTLQKKPDPFKIWAQSRSMYESRLPDCQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTPGYGVSKGEGDVPPSPASSHHSSTQAPSLTEGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGSAVVPASLQPYDVEIQRGENEGFGFVIVSSVSRPDAGTTLAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVSLRIIPGDESSNASLLTNAEKIATITTTHTPQTTELRNHSKSKGVPPPPPSQTHLQDAEYYSVDLERDSKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKNMKHSRAIELIKNGGRRARLVLKRGDGSVPEYDGSSDGYPSPSGAQNAPEVRKLPPNSRYHTSFESSYPPDFHKPSRLEEPSRGRSMEKNRDWAGSDLMDFHGRQFNPHLHHTWNNSHSQSIPRQQSPDNSNGSTVGNKKSRGRKVKKSESESGISKLLKSLRKDSSGKKAAAAEKLRGRELSSSSSTLDSKLRPQRRGSLDRSPTRKRNSSPDRRRAKSMDRRAHRDHTPEREYDDGGFLSVTPERKLYERKLLKESPLAGRVSEVSTPERVNNSADSLTRGRAYDKATKNGNLLRDSSSERREERHRGNGTMERRYRLERDSSPDSNYSRDELNYAAAAAPRLKDYYSMMKNNAAHNNATYNKTGHNNTAGRRSSNQIPEPKRRLYKENPKDLSI

Target ORF information:

RefSeq Version	XM_015383736.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X9, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015383736.1

ORF Insert Sequence:

ATGGCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACAA AAGTAAATGA GTGCACGATT 
ATCCGGGACA CGAGTGGGGA GTTGAACGTG GAGCTGCACG GTGGAGCGGA GAATGGAGAG 
TTTACATACA TTGGAGATAT CAGGGAGGAT GCTGTGGTTT ATCAGGACGG AAAGCTGTCC 
AAGGGAGAAC TACTGCTAGA CGTGGAGGGT CTGTCGGTGT CTGGATTACC CCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAGGGA CCTGTCAGGT TTAAAACTGT TCGACAAGGC 
AACAAGCTCA ACAAGGACCT AAAGCACTTC TTGAGCTTAA GGTTTCAGAA AACATCACCT 
GACCACGAGT TGCAGAAAAC CATCCGAGCC AATCTATATC GCCATGCTGT GCCTTGTACG 
ACCCGCCCCC CTCGAGACGG AGAGGTCCCA GGGTTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTGGAGGA CAGTGGCACA CTACTGGAAG TAGGAACATA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC TCCAAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
GCTGGCTCCC TTCCGTCCAC ACCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAAAGTC 
CGCGTAGTGC CTGCTGACAA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGATCCT TAGTGCGTAG TCTCTTCCCC TCTCCTTTGC TGTGCACAGG TAACCCTAAC 
GACCAGGACG AGAGCCGCAG CGGCATCCTC CGGCCGTATC CCGTGCGTGA TAATGCTCTA 
CCCTGTGGTC TGAACAATGC AGCCATTTCC ACTGCAGAGA GCGGGCAGCA GACTCTTGCA 
GAGCCCCAGG TCTCACCAGA GGACCCACTG GGACCGCTGC CTGAAAACTG GGAGATGGCC 
TACACAGAGA ACGGGGAACT GTACTTCATA GACCACAACA CAAAGACCAC TTCATGGCTG 
GATCCCCGGT GTAGAGACAA AGTCCCCAGG CCTCTGGAAG AGTGTGATGA TGATGAACTG 
CCTCCAGGAT GGGAGAGAAT AGATGACCCA GTGTACGGAG TGTATTATGT GGACCATATA 
AACAGGAAGA CCCAGTATGA GAACCCAGTT TTGGAGGCGC GGCATCAAAA AATCTTGGAG 
CAGCAGCAAC CGCCGCAGCC TCCAGAAGGT GAGCGGTATA TCCGAGAGTG GATAGAGGAG 
CATTCGTCTG CTGCAGCTCC ATTGGCAAAC TATGTTCCAA ACCACCTTGA GACATACAGG 
GACCCACAAG TCCCACCAGC TCTACCTCCT GGCCCAGTCG GGGTTAAAAG GGGGAAGCCT 
TTCTTCACCA GAAATCCTGC AGAACTGAAA GGAACCTTTA TCAACACCAA GCTAAAGAAA 
AGTCGCCGTG GATTTGGCTT CACAGTCGTT GGTGGCGATG AGCCTGATGA ATTCCTGCAG 
ATCAAAAGTC TGGTGCTGGA CGGGCCTGCA GCTGTCGATG GCAAAATGGA GACAGGGGAT 
GTGATTGTCA GTGTAAATGA CACCATCGTT CTGGGCTACA CACATGCACA AGTGGTGAAG 
ATTTTCCAGT CCATCCCTAT CGGCTCAATG GTGGACCTGG CCTTATGTCG AGGATATCCG 
CTGCCATTTG ACCCAGATGA CCCAAACACC AGCCTAGTGA CCTCTGTGGC GATCTTGGAC 
AAGGAGCCTA TCATTGTCAA TGGACAAGAG ACTTTTGATG TACCCGCCAA CCAGGCTGGA 
CCCATCAATG GCATGCGGGA GGCACGGTCG CACAGCCCCT CAGCGGAGGT GGCATCAAAC 
GGTTCACATG GGTACTCCGC TGACGTGGTC ACCCTGGCCT CAGCCATCGC CACCCAGCCA 
GAGCTGATCA CCATACACAT GGAGAAGGGA GACAAAGGGT TTGGCTTCAC CATTGCTGAC 
AGCCTGACAG GAGGCGGGCA GAGAGTCAAG CAGATAGTGG ACTACCCACG GTGCCGTGGC 
CTGAAAGAGG GGGACATTCT GATGGAGGTT AACAAGAGAA ACGTCCAGAA TATGAGCCAC 
AACCAGGTGG TTGATCTGCT CAGCAAATGT CCGAGAGGCA GCGAAGTCAC CATGTTGGTG 
CAAAGAGGCG TGGTGCCAGC CAAGAGAAGC CCAAAGCTGG TGCACCAGTT GGAGAGAAAG 
GACAGTCAGA CCAGCTCCCA GCACAGCGTT TGCAGCCATC GAAGTGCTCA CACCGACTCT 
CCAAGCCACC CACCCTCTGC CATGCCAAGT GAGGCAGTGG TCCCCACTCC TGCTGCTCCC 
ACACAGCCTC TGCCGGTCTT GCCCCCCCAA GACCCAGCAG ATGGCACTCT CACCCTCCAG 
AAGAAGCCAG ACCCATTCAA GATCTGGGCC CAGTCCAGAA GCATGTATGA AAGTCGATTG 
CCAGATTGCC AGGAGCAAGA CATTTTCCTT TGGAGGAAGG ATACTGGCTT TGGCTTCCGC 
ATTTTAGGAG GAAATGAGCC TGGAGAACCT ATTTACATTG GTCATATAGT GAAATATGGT 
GCTGCGGATG AAGATGGCCG CCTGCGGTCT GGTGATGAGC TTATTTGTGT GGACGGCACA 
GCGGTAGTGG GCAAGTCCCA CCAGCTGGTG GTTCAGCTGA TGCAGCAGGC TGCCAAACAG 
GGTCATGTAA ACCTCACCGT CAGGCGCAAG ACCCCCGGAT ATGGAGTGTC AAAGGGAGAG 
GGTGATGTTC CGCCATCGCC GGCCTCTTCC CATCACAGCA GCACTCAGGC GCCAAGCCTC 
ACAGAAGGCA AGCGGACCCC TCAGGGAAGC CAAAACTCCC TGAATACTGT TAGCTCTGGC 
AGTGGATCTA CCAGCGGTAT AGGCAGTGGT GGTGGAGGTG GCAGTGGAAG CGCTGTGGTG 
CCCGCCTCTC TCCAGCCATA CGACGTAGAG ATCCAGCGGG GAGAAAATGA GGGATTTGGT 
TTCGTCATCG TATCATCCGT CTCCCGGCCT GATGCTGGAA CCACATTGGC TGGAAATGCA 
TGCGTTGCGA TGCCCCACAA AATAGGTCGT ATCATAGAGG GCAGCCCGGC TGACCGCTGC 
GGGAAGCTGA AGGTTGGAGA CCGGATTCTG GCTGTCAATG GCTGCTCCAT CACAAACAAG 
TCACATTCGG ACATCGTCAA CCTGATCAAG GAGGCCGGGA ACACTGTCTC ACTCAGGATC 
ATTCCAGGCG ATGAATCTTC CAATGCTTCT CTCCTGACTA ATGCAGAGAA AATCGCTACC 
ATCACCACCA CACACACACC TCAAACCACA GAGTTAAGGA ATCATTCAAA GTCAAAAGGA 
GTTCCTCCTC CACCTCCTTC GCAAACTCAT CTGCAGGATG CAGAATACTA TTCTGTGGAC 
CTTGAACGGG ACAGTAAAGG CTTCGGCTTC AGCCTAAGAG GAGGACGGGA GTACAACATG 
GACCTTTATG TGCTGAGACT AGCCGAGGAT GGGGCTGCTG TCAGAAATGG CAAAATGAGG 
GTGGGAGATG AGATCCTGGA GATTAATGGT GAGAGCACAA AGAACATGAA GCATTCACGA 
GCCATTGAAC TGATCAAGAA TGGAGGTCGG AGGGCAAGGC TTGTCCTGAA GAGAGGAGAT 
GGATCTGTAC CCGAGTATGA CGGCAGCAGT GACGGGTACC CTTCCCCATC CGGGGCACAA 
AACGCTCCGG AAGTGAGAAA GCTGCCGCCC AACAGCCGAT ATCACACCTC ATTTGAGTCC 
AGTTATCCAC CAGACTTTCA CAAACCATCA CGCCTGGAGG AGCCTTCGCG TGGCCGAAGT 
ATGGAGAAGA ACAGGGACTG GGCCGGCTCA GATCTGATGG ACTTCCATGG CCGGCAGTTT 
AACCCCCACC TTCATCACAC CTGGAATAAC AGTCACAGTC AAAGTATCCC CCGACAGCAG 
TCTCCCGACA ACAGTAATGG CAGCACCGTT GGCAACAAGA AGAGCCGAGG ACGCAAAGTC 
AAGAAGTCTG AATCAGAGAG TGGCATCTCC AAGCTCCTGA AAAGTCTAAG GAAAGACAGC 
TCAGGGAAGA AGGCTGCTGC GGCCGAAAAA CTGCGAGGCA GAGAGCTCTC GAGCAGCAGT 
TCTACTCTGG ACAGTAAACT CCGTCCACAG CGTCGTGGCT CCCTTGATCG CTCACCAACC 
AGGAAGCGTA ACTCATCTCC GGATCGTCGG CGGGCAAAGT CCATGGACCG CAGAGCACAT 
CGGGACCACA CACCTGAGAG GGAATATGAT GACGGAGGGT TTCTCTCTGT AACACCTGAG 
AGAAAGCTCT ATGAACGGAA GCTCCTCAAG GAGTCACCGT TAGCCGGTCG AGTCAGTGAG 
GTCTCCACCC CTGAGCGAGT CAACAACAGT GCAGATTCTC TCACCAGAGG CCGGGCATAC 
GATAAAGCCA CTAAGAACGG AAACCTTTTG AGAGACTCTT CCTCAGAAAG GAGGGAGGAG 
AGGCACAGAG GGAATGGGAC AATGGAGAGG CGGTACAGAC TGGAGCGGGA CTCTTCCCCT 
GACAGCAACT ACAGTCGGGA CGAGCTGAAC TATGCAGCAG CAGCAGCACC CAGACTGAAG 
GACTACTACA GCATGATGAA GAACAATGCT GCACACAACA ACGCTACCTA CAACAAGACA 
GGACACAACA ATACTGCTGG AAGACGCAGC TCAAACCAGA TCCCTGAGCC CAAGAGAAGG 
CTGTACAAAG AAAACCCAAA AGACCTCAGT ATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107090390 ( XM_015383737.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015239223.1 Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X10, mRNA.

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CloneID	OCy14733
Clone ID Related Accession (Same CDS sequence)	XM_015383737.1
Accession Version	XM_015383737.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4767bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X10
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150746.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACAA AAGTAAATGA GTGCACGATT 
ATCCGGGACA CGAGTGGGGA GTTGAACGTG GAGCTGCACG GTGGAGCGGA GAATGGAGAG 
TTTACATACA TTGGAGATAT CAGGGAGGAT GCTGTGGTTT ATCAGGACGG AAAGCTGTCC 
AAGGGAGAAC TACTGCTAGA CGTGGAGGGT CTGTCGGTGT CTGGATTACC CCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAGGGA CCTGTCAGGT TTAAAACTGT TCGACAAGGC 
AACAAGCTCA ACAAGGACCT AAAGCACTTC TTGAGCTTAA GGTTTCAGAA AACATCACCT 
GACCACGAGT TGCAGAAAAC CATCCGAGCC AATCTATATC GCCATGCTGT GCCTTGTACG 
ACCCGCCCCC CTCGAGACGG AGAGGTCCCA GGGTTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTGGAGGA CAGTGGCACA CTACTGGAAG TAGGAACATA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC TCCAAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
GCTGGCTCCC TTCCGTCCAC ACCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAAAGTC 
CGCGTAGTGC CTGCTGACAA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGTAACC CTAACGACCA GGACGAGAGC CGCAGCGGCA TCCTCCGGCC GTATCCCGTG 
CGTGATAATG CTCTACCCTG TGGTCTGAAC AATGCAGCCA TTTCCACTGC AGAGAGCGGG 
CAGCAGACTC TTGCAGAGCC CCAGGTCTCA CCAGAGGACC CACTGGGACC GCTGCCTGAA 
AACTGGGAGA TGGCCTACAC AGAGAACGGG GAACTGTACT TCATAGACCA CAACACAAAG 
ACCACTTCAT GGCTGGATCC CCGGTGTAGA GACAAAGTCC CCAGGCCTCT GGAAGAGTGT 
GATGATGATG AAGGGATACA TGCGGAAGAC CTGAAAAGTG ATCTAGAACT GCCTCCAGGA 
TGGGAGAGAA TAGATGACCC AGTGTACGGA GTGTATTATG TGGACCATAT AAACAGGAAG 
ACCCAGTATG AGAACCCAGT TTTGGAGGCG CGGCATCAAA AAATCTTGGA GCAGCAGCAA 
CCGCCGCAGC CTCCAGAAGG TGAGCGGTAT ATCCGAGAGT GGATAGAGGA GCATTCGTCT 
GCTGCAGCTC CATTGGCAAA CTATGTTCCA AACCACCTTG AGACATACAG GGACCCACAA 
GTCCCACCAG CTCTACCTCC TGGCCCAGTC GGGGTTAAAA GGGGGAAGCC TTTCTTCACC 
AGAAATCCTG CAGAACTGAA AGGAACCTTT ATCAACACCA AGCTAAAGAA AAGTCGCCGT 
GGATTTGGCT TCACAGTCGT TGGTGGCGAT GAGCCTGATG AATTCCTGCA GATCAAAAGT 
CTGGTGCTGG ACGGGCCTGC AGCTGTCGAT GGCAAAATGG AGACAGGGGA TGTGATTGTC 
AGTGTAAATG ACACCATCGT TCTGGGCTAC ACACATGCAC AAGTGGTGAA GATTTTCCAG 
TCCATCCCTA TCGGCTCAAT GGTGGACCTG GCCTTATGTC GAGGATATCC GCTGCCATTT 
GACCCAGATG ACCCAAACAC CAGCCTAGTG ACCTCTGTGG CGATCTTGGA CAAGGAGCCT 
ATCATTGTCA ATGGACAAGA GACTTTTGAT GTACCCGCCA ACCAGGCTGG ACCCATCAAT 
GGCATGCGGG AGGCACGGTC GCACAGCCCC TCAGCGGAGG TGGCATCAAA CGGTTCACAT 
GGGTACTCCG CTGACGTGGT CACCCTGGCC TCAGCCATCG CCACCCAGCC AGAGCTGATC 
ACCATACACA TGGAGAAGGG AGACAAAGGG TTTGGCTTCA CCATTGCTGA CAGCCTGACA 
GGAGGCGGGC AGAGAGTCAA GCAGATAGTG GACTACCCAC GGTGCCGTGG CCTGAAAGAG 
GGGGACATTC TGATGGAGGT TAACAAGAGA AACGTCCAGA ATATGAGCCA CAACCAGGTG 
GTTGATCTGC TCAGCAAATG TCCGAGAGGC AGCGAAGTCA CCATGTTGGT GCAAAGAGGC 
GTGGTGCCAG CCAAGAGAAG CCCAAAGCTG GTGCACCAGT TGGAGAGAAA GGACAGTCAG 
ACCAGCTCCC AGCACAGCGT TTGCAGCCAT CGAAGTGCTC ACACCGACTC TCCAAGCCAC 
CCACCCTCTG CCATGCCAAG TGAGGCAGTG GTCCCCACTC CTGCTGCTCC CACACAGCCT 
CTGCCGGTCT TGCCCCCCCA AGACCCAGCA GATGGCACTC TCACCCTCCA GAAGAAGCCA 
GACCCATTCA AGATCTGGGC CCAGTCCAGA AGCATGTATG AAAGTCGATT GCCAGATTGC 
CAGGAGCAAG ACATTTTCCT TTGGAGGAAG GATACTGGCT TTGGCTTCCG CATTTTAGGA 
GGAAATGAGC CTGGAGAACC TATTTACATT GGTCATATAG TGAAATATGG TGCTGCGGAT 
GAAGATGGCC GCCTGCGGTC TGGTGATGAG CTTATTTGTG TGGACGGCAC AGCGGTAGTG 
GGCAAGTCCC ACCAGCTGGT GGTTCAGCTG ATGCAGCAGG CTGCCAAACA GGGTCATGTA 
AACCTCACCG TCAGGCGCAA GACCCCCGGA TATGGAGTGT CAAAGGGAGA GGGTGATGTT 
CCGCCATCGC CGGCCTCTTC CCATCACAGC AGCACTCAGG CGCCAAGCCT CACAGAAGGC 
AAGCGGACCC CTCAGGGAAG CCAAAACTCC CTGAATACTG TTAGCTCTGG CAGTGGATCT 
ACCAGCGGTA TAGGCAGTGG TGGTGGAGGT GGCAGTGGAA GCGCTGTGGT GCCCGCCTCT 
CTCCAGCCAT ACGACGTAGA GATCCAGCGG GGAGAAAATG AGGGATTTGG TTTCGTCATC 
GTATCATCCG TCTCCCGGCC TGATGCTGGA ACCACATTGG CTGGAAATGC ATGCGTTGCG 
ATGCCCCACA AAATAGGTCG TATCATAGAG GGCAGCCCGG CTGACCGCTG CGGGAAGCTG 
AAGGTTGGAG ACCGGATTCT GGCTGTCAAT GGCTGCTCCA TCACAAACAA GTCACATTCG 
GACATCGTCA ACCTGATCAA GGAGGCCGGG AACACTGTCT CACTCAGGAT CATTCCAGGC 
GATGAATCTT CCAATGCTTC TCTCCTGACT AATGCAGAGA AAATCGCTAC CATCACCACC 
ACACACACAC CTCAAACCAC AGAGTTAAGG AATCATTCAA AGTCAAAAGG AGTTCCTCCT 
CCACCTCCTT CGCAAACTCA TCTGCAGGAT GCAGAATACT ATTCTGTGGA CCTTGAACGG 
GACAGTAAAG GCTTCGGCTT CAGCCTAAGA GGAGGACGGG AGTACAACAT GGACCTTTAT 
GTGCTGAGAC TAGCCGAGGA TGGGGCTGCT GTCAGAAATG GCAAAATGAG GGTGGGAGAT 
GAGATCCTGG AGATTAATGG TGAGAGCACA AAGAACATGA AGCATTCACG AGCCATTGAA 
CTGATCAAGA ATGGAGGTCG GAGGGCAAGG CTTGTCCTGA AGAGAGGAGA TGGATCTGTA 
CCCGAGTATG ACGGCAGCAG TGACGGGTAC CCTTCCCCAT CCGGGGCACA AAACGCTCCG 
GAAGTGAGAA AGCTGCCGCC CAACAGCCGA TATCACACCT CATTTGAGTC CAGTTATCCA 
CCAGACTTTC ACAAACCATC ACGCCTGGAG GAGCCTTCGC GTGGCCGAAG TATGGAGAAG 
AACAGGGACT GGGCCGGCTC AGATCTGATG GACTTCCATG GCCGGCAGTT TAACCCCCAC 
CTTCATCACA CCTGGAATAA CAGTCACAGT CAAAGTATCC CCCGACAGCA GTCTCCCGAC 
AACAGTAATG GCAGCACCGT TGGCAACAAG AAGAGCCGAG GACGCAAAGT CAAGAAGTCT 
GAATCAGAGA GTGGCATCTC CAAGCTCCTG AAAAGTCTAA GGAAAGACAG CTCAGGGAAG 
AAGGCTGCTG CGGCCGAAAA ACTGCGAGGC AGAGAGCTCT CGAGCAGCAG TTCTACTCTG 
GACAGTAAAC TCCGTCCACA GCGTCGTGGC TCCCTTGATC GCTCACCAAC CAGGAAGCGT 
AACTCATCTC CGGATCGTCG GCGGGCAAAG TCCATGGACC GCAGAGCACA TCGGGACCAC 
ACACCTGAGA GGGAATATGA TGACGGAGGG TTTCTCTCTG TAACACCTGA GAGAAAGCTC 
TATGAACGGA AGCTCCTCAA GGAGTCACCG TTAGCCGGTC GAGTCAGTGA GGTCTCCACC 
CCTGAGCGAG TCAACAACAG TGCAGATTCT CTCACCAGAG GCCGGGCATA CGATAAAGCC 
ACTAAGAACG GAAACCTTTT GAGAGACTCT TCCTCAGAAA GGAGGGAGGA GAGGCACAGA 
GGGAATGGGA CAATGGAGAG GCGGTACAGA CTGGAGCGGG ACTCTTCCCC TGACAGCAAC 
TACAGTCGGG ACGAGCTGAA CTATGCAGCA GCAGCAGCAC CCAGACTGAA GGACTACTAC 
AGCATGATGA AGAACAATGC TGCACACAAC AACGCTACCT ACAACAAGAC AGGACACAAC 
AATACTGCTG GAAGACGCAG CTCAAACCAG ATCCCTGAGC CCAAGAGAAG GCTGTACAAA 
GAAAACCCAA AAGACCTCAG TATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015239223.1
CDS	572..5338
Translation	MAKVIQKKNHWITKVNECTIIRDTSGELNVELHGGAENGEFTYIGDIREDAVVYQDGKLSKGELLLDVEGLSVSGLPLYDIYTVINCCKGPVRFKTVRQGNKLNKDLKHFLSLRFQKTSPDHELQKTIRANLYRHAVPCTTRPPRDGEVPGLDYNFLSVEDFLQLEDSGTLLEVGTYEGNYYGTPKPPRQPIIGTVTLSDAGSLPSTPKRTKSYNDMQKVRVVPADNDDDDQATEMNNSFTGNPNDQDESRSGILRPYPVRDNALPCGLNNAAISTAESGQQTLAEPQVSPEDPLGPLPENWEMAYTENGELYFIDHNTKTTSWLDPRCRDKVPRPLEECDDDEGIHAEDLKSDLELPPGWERIDDPVYGVYYVDHINRKTQYENPVLEARHQKILEQQQPPQPPEGERYIREWIEEHSSAAAPLANYVPNHLETYRDPQVPPALPPGPVGVKRGKPFFTRNPAELKGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAAVDGKMETGDVIVSVNDTIVLGYTHAQVVKIFQSIPIGSMVDLALCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETFDVPANQAGPINGMREARSHSPSAEVASNGSHGYSADVVTLASAIATQPELITIHMEKGDKGFGFTIADSLTGGGQRVKQIVDYPRCRGLKEGDILMEVNKRNVQNMSHNQVVDLLSKCPRGSEVTMLVQRGVVPAKRSPKLVHQLERKDSQTSSQHSVCSHRSAHTDSPSHPPSAMPSEAVVPTPAAPTQPLPVLPPQDPADGTLTLQKKPDPFKIWAQSRSMYESRLPDCQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTPGYGVSKGEGDVPPSPASSHHSSTQAPSLTEGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGSAVVPASLQPYDVEIQRGENEGFGFVIVSSVSRPDAGTTLAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVSLRIIPGDESSNASLLTNAEKIATITTTHTPQTTELRNHSKSKGVPPPPPSQTHLQDAEYYSVDLERDSKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKNMKHSRAIELIKNGGRRARLVLKRGDGSVPEYDGSSDGYPSPSGAQNAPEVRKLPPNSRYHTSFESSYPPDFHKPSRLEEPSRGRSMEKNRDWAGSDLMDFHGRQFNPHLHHTWNNSHSQSIPRQQSPDNSNGSTVGNKKSRGRKVKKSESESGISKLLKSLRKDSSGKKAAAAEKLRGRELSSSSSTLDSKLRPQRRGSLDRSPTRKRNSSPDRRRAKSMDRRAHRDHTPEREYDDGGFLSVTPERKLYERKLLKESPLAGRVSEVSTPERVNNSADSLTRGRAYDKATKNGNLLRDSSSERREERHRGNGTMERRYRLERDSSPDSNYSRDELNYAAAAAPRLKDYYSMMKNNAAHNNATYNKTGHNNTAGRRSSNQIPEPKRRLYKENPKDLSI

Target ORF information:

RefSeq Version	XM_015383737.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X10, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015383737.1

ORF Insert Sequence:

ATGGCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACAA AAGTAAATGA GTGCACGATT 
ATCCGGGACA CGAGTGGGGA GTTGAACGTG GAGCTGCACG GTGGAGCGGA GAATGGAGAG 
TTTACATACA TTGGAGATAT CAGGGAGGAT GCTGTGGTTT ATCAGGACGG AAAGCTGTCC 
AAGGGAGAAC TACTGCTAGA CGTGGAGGGT CTGTCGGTGT CTGGATTACC CCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAGGGA CCTGTCAGGT TTAAAACTGT TCGACAAGGC 
AACAAGCTCA ACAAGGACCT AAAGCACTTC TTGAGCTTAA GGTTTCAGAA AACATCACCT 
GACCACGAGT TGCAGAAAAC CATCCGAGCC AATCTATATC GCCATGCTGT GCCTTGTACG 
ACCCGCCCCC CTCGAGACGG AGAGGTCCCA GGGTTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTGGAGGA CAGTGGCACA CTACTGGAAG TAGGAACATA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC TCCAAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
GCTGGCTCCC TTCCGTCCAC ACCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAAAGTC 
CGCGTAGTGC CTGCTGACAA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGTAACC CTAACGACCA GGACGAGAGC CGCAGCGGCA TCCTCCGGCC GTATCCCGTG 
CGTGATAATG CTCTACCCTG TGGTCTGAAC AATGCAGCCA TTTCCACTGC AGAGAGCGGG 
CAGCAGACTC TTGCAGAGCC CCAGGTCTCA CCAGAGGACC CACTGGGACC GCTGCCTGAA 
AACTGGGAGA TGGCCTACAC AGAGAACGGG GAACTGTACT TCATAGACCA CAACACAAAG 
ACCACTTCAT GGCTGGATCC CCGGTGTAGA GACAAAGTCC CCAGGCCTCT GGAAGAGTGT 
GATGATGATG AAGGGATACA TGCGGAAGAC CTGAAAAGTG ATCTAGAACT GCCTCCAGGA 
TGGGAGAGAA TAGATGACCC AGTGTACGGA GTGTATTATG TGGACCATAT AAACAGGAAG 
ACCCAGTATG AGAACCCAGT TTTGGAGGCG CGGCATCAAA AAATCTTGGA GCAGCAGCAA 
CCGCCGCAGC CTCCAGAAGG TGAGCGGTAT ATCCGAGAGT GGATAGAGGA GCATTCGTCT 
GCTGCAGCTC CATTGGCAAA CTATGTTCCA AACCACCTTG AGACATACAG GGACCCACAA 
GTCCCACCAG CTCTACCTCC TGGCCCAGTC GGGGTTAAAA GGGGGAAGCC TTTCTTCACC 
AGAAATCCTG CAGAACTGAA AGGAACCTTT ATCAACACCA AGCTAAAGAA AAGTCGCCGT 
GGATTTGGCT TCACAGTCGT TGGTGGCGAT GAGCCTGATG AATTCCTGCA GATCAAAAGT 
CTGGTGCTGG ACGGGCCTGC AGCTGTCGAT GGCAAAATGG AGACAGGGGA TGTGATTGTC 
AGTGTAAATG ACACCATCGT TCTGGGCTAC ACACATGCAC AAGTGGTGAA GATTTTCCAG 
TCCATCCCTA TCGGCTCAAT GGTGGACCTG GCCTTATGTC GAGGATATCC GCTGCCATTT 
GACCCAGATG ACCCAAACAC CAGCCTAGTG ACCTCTGTGG CGATCTTGGA CAAGGAGCCT 
ATCATTGTCA ATGGACAAGA GACTTTTGAT GTACCCGCCA ACCAGGCTGG ACCCATCAAT 
GGCATGCGGG AGGCACGGTC GCACAGCCCC TCAGCGGAGG TGGCATCAAA CGGTTCACAT 
GGGTACTCCG CTGACGTGGT CACCCTGGCC TCAGCCATCG CCACCCAGCC AGAGCTGATC 
ACCATACACA TGGAGAAGGG AGACAAAGGG TTTGGCTTCA CCATTGCTGA CAGCCTGACA 
GGAGGCGGGC AGAGAGTCAA GCAGATAGTG GACTACCCAC GGTGCCGTGG CCTGAAAGAG 
GGGGACATTC TGATGGAGGT TAACAAGAGA AACGTCCAGA ATATGAGCCA CAACCAGGTG 
GTTGATCTGC TCAGCAAATG TCCGAGAGGC AGCGAAGTCA CCATGTTGGT GCAAAGAGGC 
GTGGTGCCAG CCAAGAGAAG CCCAAAGCTG GTGCACCAGT TGGAGAGAAA GGACAGTCAG 
ACCAGCTCCC AGCACAGCGT TTGCAGCCAT CGAAGTGCTC ACACCGACTC TCCAAGCCAC 
CCACCCTCTG CCATGCCAAG TGAGGCAGTG GTCCCCACTC CTGCTGCTCC CACACAGCCT 
CTGCCGGTCT TGCCCCCCCA AGACCCAGCA GATGGCACTC TCACCCTCCA GAAGAAGCCA 
GACCCATTCA AGATCTGGGC CCAGTCCAGA AGCATGTATG AAAGTCGATT GCCAGATTGC 
CAGGAGCAAG ACATTTTCCT TTGGAGGAAG GATACTGGCT TTGGCTTCCG CATTTTAGGA 
GGAAATGAGC CTGGAGAACC TATTTACATT GGTCATATAG TGAAATATGG TGCTGCGGAT 
GAAGATGGCC GCCTGCGGTC TGGTGATGAG CTTATTTGTG TGGACGGCAC AGCGGTAGTG 
GGCAAGTCCC ACCAGCTGGT GGTTCAGCTG ATGCAGCAGG CTGCCAAACA GGGTCATGTA 
AACCTCACCG TCAGGCGCAA GACCCCCGGA TATGGAGTGT CAAAGGGAGA GGGTGATGTT 
CCGCCATCGC CGGCCTCTTC CCATCACAGC AGCACTCAGG CGCCAAGCCT CACAGAAGGC 
AAGCGGACCC CTCAGGGAAG CCAAAACTCC CTGAATACTG TTAGCTCTGG CAGTGGATCT 
ACCAGCGGTA TAGGCAGTGG TGGTGGAGGT GGCAGTGGAA GCGCTGTGGT GCCCGCCTCT 
CTCCAGCCAT ACGACGTAGA GATCCAGCGG GGAGAAAATG AGGGATTTGG TTTCGTCATC 
GTATCATCCG TCTCCCGGCC TGATGCTGGA ACCACATTGG CTGGAAATGC ATGCGTTGCG 
ATGCCCCACA AAATAGGTCG TATCATAGAG GGCAGCCCGG CTGACCGCTG CGGGAAGCTG 
AAGGTTGGAG ACCGGATTCT GGCTGTCAAT GGCTGCTCCA TCACAAACAA GTCACATTCG 
GACATCGTCA ACCTGATCAA GGAGGCCGGG AACACTGTCT CACTCAGGAT CATTCCAGGC 
GATGAATCTT CCAATGCTTC TCTCCTGACT AATGCAGAGA AAATCGCTAC CATCACCACC 
ACACACACAC CTCAAACCAC AGAGTTAAGG AATCATTCAA AGTCAAAAGG AGTTCCTCCT 
CCACCTCCTT CGCAAACTCA TCTGCAGGAT GCAGAATACT ATTCTGTGGA CCTTGAACGG 
GACAGTAAAG GCTTCGGCTT CAGCCTAAGA GGAGGACGGG AGTACAACAT GGACCTTTAT 
GTGCTGAGAC TAGCCGAGGA TGGGGCTGCT GTCAGAAATG GCAAAATGAG GGTGGGAGAT 
GAGATCCTGG AGATTAATGG TGAGAGCACA AAGAACATGA AGCATTCACG AGCCATTGAA 
CTGATCAAGA ATGGAGGTCG GAGGGCAAGG CTTGTCCTGA AGAGAGGAGA TGGATCTGTA 
CCCGAGTATG ACGGCAGCAG TGACGGGTAC CCTTCCCCAT CCGGGGCACA AAACGCTCCG 
GAAGTGAGAA AGCTGCCGCC CAACAGCCGA TATCACACCT CATTTGAGTC CAGTTATCCA 
CCAGACTTTC ACAAACCATC ACGCCTGGAG GAGCCTTCGC GTGGCCGAAG TATGGAGAAG 
AACAGGGACT GGGCCGGCTC AGATCTGATG GACTTCCATG GCCGGCAGTT TAACCCCCAC 
CTTCATCACA CCTGGAATAA CAGTCACAGT CAAAGTATCC CCCGACAGCA GTCTCCCGAC 
AACAGTAATG GCAGCACCGT TGGCAACAAG AAGAGCCGAG GACGCAAAGT CAAGAAGTCT 
GAATCAGAGA GTGGCATCTC CAAGCTCCTG AAAAGTCTAA GGAAAGACAG CTCAGGGAAG 
AAGGCTGCTG CGGCCGAAAA ACTGCGAGGC AGAGAGCTCT CGAGCAGCAG TTCTACTCTG 
GACAGTAAAC TCCGTCCACA GCGTCGTGGC TCCCTTGATC GCTCACCAAC CAGGAAGCGT 
AACTCATCTC CGGATCGTCG GCGGGCAAAG TCCATGGACC GCAGAGCACA TCGGGACCAC 
ACACCTGAGA GGGAATATGA TGACGGAGGG TTTCTCTCTG TAACACCTGA GAGAAAGCTC 
TATGAACGGA AGCTCCTCAA GGAGTCACCG TTAGCCGGTC GAGTCAGTGA GGTCTCCACC 
CCTGAGCGAG TCAACAACAG TGCAGATTCT CTCACCAGAG GCCGGGCATA CGATAAAGCC 
ACTAAGAACG GAAACCTTTT GAGAGACTCT TCCTCAGAAA GGAGGGAGGA GAGGCACAGA 
GGGAATGGGA CAATGGAGAG GCGGTACAGA CTGGAGCGGG ACTCTTCCCC TGACAGCAAC 
TACAGTCGGG ACGAGCTGAA CTATGCAGCA GCAGCAGCAC CCAGACTGAA GGACTACTAC 
AGCATGATGA AGAACAATGC TGCACACAAC AACGCTACCT ACAACAAGAC AGGACACAAC 
AATACTGCTG GAAGACGCAG CTCAAACCAG ATCCCTGAGC CCAAGAGAAG GCTGTACAAA 
GAAAACCCAA AAGACCTCAG TATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

LOC107090390 ( XM_015383739.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015239225.1 Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X11, mRNA.

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CloneID	OCy14734
Clone ID Related Accession (Same CDS sequence)	XM_015383739.1
Accession Version	XM_015383739.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 4749bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like isoform X11
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150746.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGGCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACAA AAGTAAATGA GTGCACGATT 
ATCCGGGACA CGAGTGGGGA GTTGAACGTG GAGCTGCACG GTGGAGCGGA GAATGGAGAG 
TTTACATACA TTGGAGATAT CAGGGAGGAT GCTGTGGTTT ATCAGGACGG AAAGCTGTCC 
AAGGGAGAAC TACTGCTAGA CGTGGAGGGT CTGTCGGTGT CTGGATTACC CCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAGGGA CCTGTCAGGT TTAAAACTGT TCGACAAGGC 
AACAAGCTCA ACAAGGACCT AAAGCACTTC TTGAGCTTAA GGTTTCAGAA AACATCACCT 
GACCACGAGT TGCAGAAAAC CATCCGAGCC AATCTATATC GCCATGCTGT GCCTTGTACG 
ACCCGCCCCC CTCGAGACGG AGAGGTCCCA GGGTTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTGGAGGA CAGTGGCACA CTACTGGAAG TAGGAACATA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC TCCAAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
GCTGGCTCCC TTCCGTCCAC ACCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAAAGTC 
CGCGTAGTGC CTGCTGACAA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGTAACC CTAACGACCA GGACGAGAGC CGCAGCGGCA TCCTCCGGCC GTATCCCGTG 
CGTGATAATG CTCTACCCTG TGGTCTGAAC AATGCAGCCA TTTCCACTGC AGAGAGCGGG 
CAGCAGACTC TTGCAGAGCC CCAGGTCTCA CCAGAGGACC CACTGGGACC GCTGCCTGAA 
AACTGGGAGA TGGCCTACAC AGAGAACGGG GAACTGTACT TCATAGACCA CAACACAAAG 
ACCACTTCAT GGCTGGATCC CCGGTGTAGA GACAAAGTCC CCAGGCCTCT GGAAGAGTGT 
GATGATGATG AAGGGATACA TGCGGAAGAC CTGAAAAGTG ATCTAGAACT GCCTCCAGGA 
TGGGAGAGAA TAGATGACCC AGTGTACGGA GTGTATTATG TGGACCATAT AAACAGGAAG 
ACCCAGTATG AGAACCCAGT TTTGGAGGCG CGGCATCAAA AAATCTTGGA GCAGCAGCAA 
CCGCCGCAGC CTCCAGAAGA GTGGATAGAG GAGCATTCGT CTGCTGCAGC TCCATTGGCA 
AACTATGTTC CAAACCACCT TGAGACATAC AGGGACCCAC AAGTCCCACC AGCTCTACCT 
CCTGGCCCAG TCGGGGTTAA AAGGGGGAAG CCTTTCTTCA CCAGAAATCC TGCAGAACTG 
AAAGGAACCT TTATCAACAC CAAGCTAAAG AAAAGTCGCC GTGGATTTGG CTTCACAGTC 
GTTGGTGGCG ATGAGCCTGA TGAATTCCTG CAGATCAAAA GTCTGGTGCT GGACGGGCCT 
GCAGCTGTCG ATGGCAAAAT GGAGACAGGG GATGTGATTG TCAGTGTAAA TGACACCATC 
GTTCTGGGCT ACACACATGC ACAAGTGGTG AAGATTTTCC AGTCCATCCC TATCGGCTCA 
ATGGTGGACC TGGCCTTATG TCGAGGATAT CCGCTGCCAT TTGACCCAGA TGACCCAAAC 
ACCAGCCTAG TGACCTCTGT GGCGATCTTG GACAAGGAGC CTATCATTGT CAATGGACAA 
GAGACTTTTG ATGTACCCGC CAACCAGGCT GGACCCATCA ATGGCATGCG GGAGGCACGG 
TCGCACAGCC CCTCAGCGGA GGTGGCATCA AACGGTTCAC ATGGGTACTC CGCTGACGTG 
GTCACCCTGG CCTCAGCCAT CGCCACCCAG CCAGAGCTGA TCACCATACA CATGGAGAAG 
GGAGACAAAG GGTTTGGCTT CACCATTGCT GACAGCCTGA CAGGAGGCGG GCAGAGAGTC 
AAGCAGATAG TGGACTACCC ACGGTGCCGT GGCCTGAAAG AGGGGGACAT TCTGATGGAG 
GTTAACAAGA GAAACGTCCA GAATATGAGC CACAACCAGG TGGTTGATCT GCTCAGCAAA 
TGTCCGAGAG GCAGCGAAGT CACCATGTTG GTGCAAAGAG GCGTGGTGCC AGCCAAGAGA 
AGCCCAAAGC TGGTGCACCA GTTGGAGAGA AAGGACAGTC AGACCAGCTC CCAGCACAGC 
GTTTGCAGCC ATCGAAGTGC TCACACCGAC TCTCCAAGCC ACCCACCCTC TGCCATGCCA 
AGTGAGGCAG TGGTCCCCAC TCCTGCTGCT CCCACACAGC CTCTGCCGGT CTTGCCCCCC 
CAAGACCCAG CAGATGGCAC TCTCACCCTC CAGAAGAAGC CAGACCCATT CAAGATCTGG 
GCCCAGTCCA GAAGCATGTA TGAAAGTCGA TTGCCAGATT GCCAGGAGCA AGACATTTTC 
CTTTGGAGGA AGGATACTGG CTTTGGCTTC CGCATTTTAG GAGGAAATGA GCCTGGAGAA 
CCTATTTACA TTGGTCATAT AGTGAAATAT GGTGCTGCGG ATGAAGATGG CCGCCTGCGG 
TCTGGTGATG AGCTTATTTG TGTGGACGGC ACAGCGGTAG TGGGCAAGTC CCACCAGCTG 
GTGGTTCAGC TGATGCAGCA GGCTGCCAAA CAGGGTCATG TAAACCTCAC CGTCAGGCGC 
AAGACCCCCG GATATGGAGT GTCAAAGGGA GAGGGTGATG TTCCGCCATC GCCGGCCTCT 
TCCCATCACA GCAGCACTCA GGCGCCAAGC CTCACAGAAG GCAAGCGGAC CCCTCAGGGA 
AGCCAAAACT CCCTGAATAC TGTTAGCTCT GGCAGTGGAT CTACCAGCGG TATAGGCAGT 
GGTGGTGGAG GTGGCAGTGG AAGCGCTGTG GTGCCCGCCT CTCTCCAGCC ATACGACGTA 
GAGATCCAGC GGGGAGAAAA TGAGGGATTT GGTTTCGTCA TCGTATCATC CGTCTCCCGG 
CCTGATGCTG GAACCACATT GGCTGGAAAT GCATGCGTTG CGATGCCCCA CAAAATAGGT 
CGTATCATAG AGGGCAGCCC GGCTGACCGC TGCGGGAAGC TGAAGGTTGG AGACCGGATT 
CTGGCTGTCA ATGGCTGCTC CATCACAAAC AAGTCACATT CGGACATCGT CAACCTGATC 
AAGGAGGCCG GGAACACTGT CTCACTCAGG ATCATTCCAG GCGATGAATC TTCCAATGCT 
TCTCTCCTGA CTAATGCAGA GAAAATCGCT ACCATCACCA CCACACACAC ACCTCAAACC 
ACAGAGTTAA GGAATCATTC AAAGTCAAAA GGAGTTCCTC CTCCACCTCC TTCGCAAACT 
CATCTGCAGG ATGCAGAATA CTATTCTGTG GACCTTGAAC GGGACAGTAA AGGCTTCGGC 
TTCAGCCTAA GAGGAGGACG GGAGTACAAC ATGGACCTTT ATGTGCTGAG ACTAGCCGAG 
GATGGGGCTG CTGTCAGAAA TGGCAAAATG AGGGTGGGAG ATGAGATCCT GGAGATTAAT 
GGTGAGAGCA CAAAGAACAT GAAGCATTCA CGAGCCATTG AACTGATCAA GAATGGAGGT 
CGGAGGGCAA GGCTTGTCCT GAAGAGAGGA GATGGATCTG TACCCGAGTA TGACGGCAGC 
AGTGACGGGT ACCCTTCCCC ATCCGGGGCA CAAAACGCTC CGGAAGTGAG AAAGCTGCCG 
CCCAACAGCC GATATCACAC CTCATTTGAG TCCAGTTATC CACCAGACTT TCACAAACCA 
TCACGCCTGG AGGAGCCTTC GCGTGGCCGA AGTATGGAGA AGAACAGGGA CTGGGCCGGC 
TCAGATCTGA TGGACTTCCA TGGCCGGCAG TTTAACCCCC ACCTTCATCA CACCTGGAAT 
AACAGTCACA GTCAAAGTAT CCCCCGACAG CAGTCTCCCG ACAACAGTAA TGGCAGCACC 
GTTGGCAACA AGAAGAGCCG AGGACGCAAA GTCAAGAAGT CTGAATCAGA GAGTGGCATC 
TCCAAGCTCC TGAAAAGTCT AAGGAAAGAC AGCTCAGGGA AGAAGGCTGC TGCGGCCGAA 
AAACTGCGAG GCAGAGAGCT CTCGAGCAGC AGTTCTACTC TGGACAGTAA ACTCCGTCCA 
CAGCGTCGTG GCTCCCTTGA TCGCTCACCA ACCAGGAAGC GTAACTCATC TCCGGATCGT 
CGGCGGGCAA AGTCCATGGA CCGCAGAGCA CATCGGGACC ACACACCTGA GAGGGAATAT 
GATGACGGAG GGTTTCTCTC TGTAACACCT GAGAGAAAGC TCTATGAACG GAAGCTCCTC 
AAGGAGTCAC CGTTAGCCGG TCGAGTCAGT GAGGTCTCCA CCCCTGAGCG AGTCAACAAC 
AGTGCAGATT CTCTCACCAG AGGCCGGGCA TACGATAAAG CCACTAAGAA CGGAAACCTT 
TTGAGAGACT CTTCCTCAGA AAGGAGGGAG GAGAGGCACA GAGGGAATGG GACAATGGAG 
AGGCGGTACA GACTGGAGCG GGACTCTTCC CCTGACAGCA ACTACAGTCG GGACGAGCTG 
AACTATGCAG CAGCAGCAGC ACCCAGACTG AAGGACTACT ACAGCATGAT GAAGAACAAT 
GCTGCACACA ACAACGCTAC CTACAACAAG ACAGGACACA ACAATACTGC TGGAAGACGC 
AGCTCAAACC AGATCCCTGA GCCCAAGAGA AGGCTGTACA AAGAAAACCC AAAAGACCTC 
AGTATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015239225.1
CDS	572..5320
Translation	MAKVIQKKNHWITKVNECTIIRDTSGELNVELHGGAENGEFTYIGDIREDAVVYQDGKLSKGELLLDVEGLSVSGLPLYDIYTVINCCKGPVRFKTVRQGNKLNKDLKHFLSLRFQKTSPDHELQKTIRANLYRHAVPCTTRPPRDGEVPGLDYNFLSVEDFLQLEDSGTLLEVGTYEGNYYGTPKPPRQPIIGTVTLSDAGSLPSTPKRTKSYNDMQKVRVVPADNDDDDQATEMNNSFTGNPNDQDESRSGILRPYPVRDNALPCGLNNAAISTAESGQQTLAEPQVSPEDPLGPLPENWEMAYTENGELYFIDHNTKTTSWLDPRCRDKVPRPLEECDDDEGIHAEDLKSDLELPPGWERIDDPVYGVYYVDHINRKTQYENPVLEARHQKILEQQQPPQPPEEWIEEHSSAAAPLANYVPNHLETYRDPQVPPALPPGPVGVKRGKPFFTRNPAELKGTFINTKLKKSRRGFGFTVVGGDEPDEFLQIKSLVLDGPAAVDGKMETGDVIVSVNDTIVLGYTHAQVVKIFQSIPIGSMVDLALCRGYPLPFDPDDPNTSLVTSVAILDKEPIIVNGQETFDVPANQAGPINGMREARSHSPSAEVASNGSHGYSADVVTLASAIATQPELITIHMEKGDKGFGFTIADSLTGGGQRVKQIVDYPRCRGLKEGDILMEVNKRNVQNMSHNQVVDLLSKCPRGSEVTMLVQRGVVPAKRSPKLVHQLERKDSQTSSQHSVCSHRSAHTDSPSHPPSAMPSEAVVPTPAAPTQPLPVLPPQDPADGTLTLQKKPDPFKIWAQSRSMYESRLPDCQEQDIFLWRKDTGFGFRILGGNEPGEPIYIGHIVKYGAADEDGRLRSGDELICVDGTAVVGKSHQLVVQLMQQAAKQGHVNLTVRRKTPGYGVSKGEGDVPPSPASSHHSSTQAPSLTEGKRTPQGSQNSLNTVSSGSGSTSGIGSGGGGGSGSAVVPASLQPYDVEIQRGENEGFGFVIVSSVSRPDAGTTLAGNACVAMPHKIGRIIEGSPADRCGKLKVGDRILAVNGCSITNKSHSDIVNLIKEAGNTVSLRIIPGDESSNASLLTNAEKIATITTTHTPQTTELRNHSKSKGVPPPPPSQTHLQDAEYYSVDLERDSKGFGFSLRGGREYNMDLYVLRLAEDGAAVRNGKMRVGDEILEINGESTKNMKHSRAIELIKNGGRRARLVLKRGDGSVPEYDGSSDGYPSPSGAQNAPEVRKLPPNSRYHTSFESSYPPDFHKPSRLEEPSRGRSMEKNRDWAGSDLMDFHGRQFNPHLHHTWNNSHSQSIPRQQSPDNSNGSTVGNKKSRGRKVKKSESESGISKLLKSLRKDSSGKKAAAAEKLRGRELSSSSSTLDSKLRPQRRGSLDRSPTRKRNSSPDRRRAKSMDRRAHRDHTPEREYDDGGFLSVTPERKLYERKLLKESPLAGRVSEVSTPERVNNSADSLTRGRAYDKATKNGNLLRDSSSERREERHRGNGTMERRYRLERDSSPDSNYSRDELNYAAAAAPRLKDYYSMMKNNAAHNNATYNKTGHNNTAGRRSSNQIPEPKRRLYKENPKDLSI

Target ORF information:

RefSeq Version	XM_015383739.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus membrane-associated guanylate kinase, WW and PDZ domain-containing protein 1-like (LOC107090390), transcript variant X11, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015383739.1

ORF Insert Sequence:

ATGGCAAAAG TCATTCAGAA GAAGAACCAC TGGATCACAA AAGTAAATGA GTGCACGATT 
ATCCGGGACA CGAGTGGGGA GTTGAACGTG GAGCTGCACG GTGGAGCGGA GAATGGAGAG 
TTTACATACA TTGGAGATAT CAGGGAGGAT GCTGTGGTTT ATCAGGACGG AAAGCTGTCC 
AAGGGAGAAC TACTGCTAGA CGTGGAGGGT CTGTCGGTGT CTGGATTACC CCTTTATGAC 
ATCTACACTG TGATCAACTG CTGCAAGGGA CCTGTCAGGT TTAAAACTGT TCGACAAGGC 
AACAAGCTCA ACAAGGACCT AAAGCACTTC TTGAGCTTAA GGTTTCAGAA AACATCACCT 
GACCACGAGT TGCAGAAAAC CATCCGAGCC AATCTATATC GCCATGCTGT GCCTTGTACG 
ACCCGCCCCC CTCGAGACGG AGAGGTCCCA GGGTTGGATT ACAACTTCCT GTCTGTGGAG 
GACTTCCTAC AACTGGAGGA CAGTGGCACA CTACTGGAAG TAGGAACATA TGAAGGTAAC 
TATTATGGGA CCCCAAAGCC TCCAAGGCAG CCCATCATTG GGACAGTGAC TCTCAGTGAT 
GCTGGCTCCC TTCCGTCCAC ACCAAAGCGC ACCAAGTCCT ACAATGACAT GCAAAAAGTC 
CGCGTAGTGC CTGCTGACAA CGACGATGAC GACCAGGCCA CGGAAATGAA CAACAGTTTT 
ACAGGTAACC CTAACGACCA GGACGAGAGC CGCAGCGGCA TCCTCCGGCC GTATCCCGTG 
CGTGATAATG CTCTACCCTG TGGTCTGAAC AATGCAGCCA TTTCCACTGC AGAGAGCGGG 
CAGCAGACTC TTGCAGAGCC CCAGGTCTCA CCAGAGGACC CACTGGGACC GCTGCCTGAA 
AACTGGGAGA TGGCCTACAC AGAGAACGGG GAACTGTACT TCATAGACCA CAACACAAAG 
ACCACTTCAT GGCTGGATCC CCGGTGTAGA GACAAAGTCC CCAGGCCTCT GGAAGAGTGT 
GATGATGATG AAGGGATACA TGCGGAAGAC CTGAAAAGTG ATCTAGAACT GCCTCCAGGA 
TGGGAGAGAA TAGATGACCC AGTGTACGGA GTGTATTATG TGGACCATAT AAACAGGAAG 
ACCCAGTATG AGAACCCAGT TTTGGAGGCG CGGCATCAAA AAATCTTGGA GCAGCAGCAA 
CCGCCGCAGC CTCCAGAAGA GTGGATAGAG GAGCATTCGT CTGCTGCAGC TCCATTGGCA 
AACTATGTTC CAAACCACCT TGAGACATAC AGGGACCCAC AAGTCCCACC AGCTCTACCT 
CCTGGCCCAG TCGGGGTTAA AAGGGGGAAG CCTTTCTTCA CCAGAAATCC TGCAGAACTG 
AAAGGAACCT TTATCAACAC CAAGCTAAAG AAAAGTCGCC GTGGATTTGG CTTCACAGTC 
GTTGGTGGCG ATGAGCCTGA TGAATTCCTG CAGATCAAAA GTCTGGTGCT GGACGGGCCT 
GCAGCTGTCG ATGGCAAAAT GGAGACAGGG GATGTGATTG TCAGTGTAAA TGACACCATC 
GTTCTGGGCT ACACACATGC ACAAGTGGTG AAGATTTTCC AGTCCATCCC TATCGGCTCA 
ATGGTGGACC TGGCCTTATG TCGAGGATAT CCGCTGCCAT TTGACCCAGA TGACCCAAAC 
ACCAGCCTAG TGACCTCTGT GGCGATCTTG GACAAGGAGC CTATCATTGT CAATGGACAA 
GAGACTTTTG ATGTACCCGC CAACCAGGCT GGACCCATCA ATGGCATGCG GGAGGCACGG 
TCGCACAGCC CCTCAGCGGA GGTGGCATCA AACGGTTCAC ATGGGTACTC CGCTGACGTG 
GTCACCCTGG CCTCAGCCAT CGCCACCCAG CCAGAGCTGA TCACCATACA CATGGAGAAG 
GGAGACAAAG GGTTTGGCTT CACCATTGCT GACAGCCTGA CAGGAGGCGG GCAGAGAGTC 
AAGCAGATAG TGGACTACCC ACGGTGCCGT GGCCTGAAAG AGGGGGACAT TCTGATGGAG 
GTTAACAAGA GAAACGTCCA GAATATGAGC CACAACCAGG TGGTTGATCT GCTCAGCAAA 
TGTCCGAGAG GCAGCGAAGT CACCATGTTG GTGCAAAGAG GCGTGGTGCC AGCCAAGAGA 
AGCCCAAAGC TGGTGCACCA GTTGGAGAGA AAGGACAGTC AGACCAGCTC CCAGCACAGC 
GTTTGCAGCC ATCGAAGTGC TCACACCGAC TCTCCAAGCC ACCCACCCTC TGCCATGCCA 
AGTGAGGCAG TGGTCCCCAC TCCTGCTGCT CCCACACAGC CTCTGCCGGT CTTGCCCCCC 
CAAGACCCAG CAGATGGCAC TCTCACCCTC CAGAAGAAGC CAGACCCATT CAAGATCTGG 
GCCCAGTCCA GAAGCATGTA TGAAAGTCGA TTGCCAGATT GCCAGGAGCA AGACATTTTC 
CTTTGGAGGA AGGATACTGG CTTTGGCTTC CGCATTTTAG GAGGAAATGA GCCTGGAGAA 
CCTATTTACA TTGGTCATAT AGTGAAATAT GGTGCTGCGG ATGAAGATGG CCGCCTGCGG 
TCTGGTGATG AGCTTATTTG TGTGGACGGC ACAGCGGTAG TGGGCAAGTC CCACCAGCTG 
GTGGTTCAGC TGATGCAGCA GGCTGCCAAA CAGGGTCATG TAAACCTCAC CGTCAGGCGC 
AAGACCCCCG GATATGGAGT GTCAAAGGGA GAGGGTGATG TTCCGCCATC GCCGGCCTCT 
TCCCATCACA GCAGCACTCA GGCGCCAAGC CTCACAGAAG GCAAGCGGAC CCCTCAGGGA 
AGCCAAAACT CCCTGAATAC TGTTAGCTCT GGCAGTGGAT CTACCAGCGG TATAGGCAGT 
GGTGGTGGAG GTGGCAGTGG AAGCGCTGTG GTGCCCGCCT CTCTCCAGCC ATACGACGTA 
GAGATCCAGC GGGGAGAAAA TGAGGGATTT GGTTTCGTCA TCGTATCATC CGTCTCCCGG 
CCTGATGCTG GAACCACATT GGCTGGAAAT GCATGCGTTG CGATGCCCCA CAAAATAGGT 
CGTATCATAG AGGGCAGCCC GGCTGACCGC TGCGGGAAGC TGAAGGTTGG AGACCGGATT 
CTGGCTGTCA ATGGCTGCTC CATCACAAAC AAGTCACATT CGGACATCGT CAACCTGATC 
AAGGAGGCCG GGAACACTGT CTCACTCAGG ATCATTCCAG GCGATGAATC TTCCAATGCT 
TCTCTCCTGA CTAATGCAGA GAAAATCGCT ACCATCACCA CCACACACAC ACCTCAAACC 
ACAGAGTTAA GGAATCATTC AAAGTCAAAA GGAGTTCCTC CTCCACCTCC TTCGCAAACT 
CATCTGCAGG ATGCAGAATA CTATTCTGTG GACCTTGAAC GGGACAGTAA AGGCTTCGGC 
TTCAGCCTAA GAGGAGGACG GGAGTACAAC ATGGACCTTT ATGTGCTGAG ACTAGCCGAG 
GATGGGGCTG CTGTCAGAAA TGGCAAAATG AGGGTGGGAG ATGAGATCCT GGAGATTAAT 
GGTGAGAGCA CAAAGAACAT GAAGCATTCA CGAGCCATTG AACTGATCAA GAATGGAGGT 
CGGAGGGCAA GGCTTGTCCT GAAGAGAGGA GATGGATCTG TACCCGAGTA TGACGGCAGC 
AGTGACGGGT ACCCTTCCCC ATCCGGGGCA CAAAACGCTC CGGAAGTGAG AAAGCTGCCG 
CCCAACAGCC GATATCACAC CTCATTTGAG TCCAGTTATC CACCAGACTT TCACAAACCA 
TCACGCCTGG AGGAGCCTTC GCGTGGCCGA AGTATGGAGA AGAACAGGGA CTGGGCCGGC 
TCAGATCTGA TGGACTTCCA TGGCCGGCAG TTTAACCCCC ACCTTCATCA CACCTGGAAT 
AACAGTCACA GTCAAAGTAT CCCCCGACAG CAGTCTCCCG ACAACAGTAA TGGCAGCACC 
GTTGGCAACA AGAAGAGCCG AGGACGCAAA GTCAAGAAGT CTGAATCAGA GAGTGGCATC 
TCCAAGCTCC TGAAAAGTCT AAGGAAAGAC AGCTCAGGGA AGAAGGCTGC TGCGGCCGAA 
AAACTGCGAG GCAGAGAGCT CTCGAGCAGC AGTTCTACTC TGGACAGTAA ACTCCGTCCA 
CAGCGTCGTG GCTCCCTTGA TCGCTCACCA ACCAGGAAGC GTAACTCATC TCCGGATCGT 
CGGCGGGCAA AGTCCATGGA CCGCAGAGCA CATCGGGACC ACACACCTGA GAGGGAATAT 
GATGACGGAG GGTTTCTCTC TGTAACACCT GAGAGAAAGC TCTATGAACG GAAGCTCCTC 
AAGGAGTCAC CGTTAGCCGG TCGAGTCAGT GAGGTCTCCA CCCCTGAGCG AGTCAACAAC 
AGTGCAGATT CTCTCACCAG AGGCCGGGCA TACGATAAAG CCACTAAGAA CGGAAACCTT 
TTGAGAGACT CTTCCTCAGA AAGGAGGGAG GAGAGGCACA GAGGGAATGG GACAATGGAG 
AGGCGGTACA GACTGGAGCG GGACTCTTCC CCTGACAGCA ACTACAGTCG GGACGAGCTG 
AACTATGCAG CAGCAGCAGC ACCCAGACTG AAGGACTACT ACAGCATGAT GAAGAACAAT 
GCTGCACACA ACAACGCTAC CTACAACAAG ACAGGACACA ACAATACTGC TGGAAGACGC 
AGCTCAAACC AGATCCCTGA GCCCAAGAGA AGGCTGTACA AAGAAAACCC AAAAGACCTC 
AGTATCTAG

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

Next-day Shipping ORF Clones ( in default vector with tag)
1 Clone	30% OFF
2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
30% OFF

LOC107090390 cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow)

Gene

Clones

gRNAs

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

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Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

Target ORF information:

Target ORF information:

ORF Insert Sequence:

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