mtif2 cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow)

Gene
Clones
gRNAs

Summary

Gene Symbol	mtif2
Entrez Gene ID	107082636
Full Name	mitochondrial translational initiation factor 2
Gene Type	protein-coding
Organism	Cyprinodon variegatus(sheepshead minnow)

mRNA and Protein(s)

mRNA	Protein	Name
XM_015371421.1	XP_015226907.1	translation initiation factor IF-2, mitochondrial

Pathways from BioSystems
Gene Ontology

The following mtif2 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the mtif2 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCy04825	XM_015371421.1 Latest version!	Cyprinodon variegatus mitochondrial translational initiation factor 2 (mtif2), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$426.30 $609.00

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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

mtif2 ( XM_015371421.1 ) cDNA ORF clone, Cyprinodon variegatus(sheepshead minnow) -> XP_015226907.1 Cyprinodon variegatus mitochondrial translational initiation factor 2 (mtif2), mRNA.

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CloneID	OCy04825
Clone ID Related Accession (Same CDS sequence)	XM_015371421.1
Accession Version	XM_015371421.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2109bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2016-01-13
Organism	Cyprinodon variegatus(sheepshead minnow)
Product	translation initiation factor IF-2, mitochondrial
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_015150511.1) annotated using gene prediction method: Gnomon. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Version :: Cyprinodon variegatus Annotation Release 100 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 6.5 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGAATGTGA TGTCATCAGT GATGCGGGGA GCAAGGCTGC TGTCCCACAC AGACATGCAC 
CTGCTCCTCT GTCCTTCCAG GAGCTCCGCC CCCCTGCTCA CCTGGACACA GAGCAGAAGG 
TTTGCCTCCA AACAGGTGAA AGGTCAGAGG AAGGACCCCA AGAAGCAGAA CCCAAAGCCC 
AAAGTGGACA AACAGGAAGT GGAGATCAGA CAGGGAATGA CGGCGGCAGC GCTAGCAGCA 
GCCATGAACA AAGACTTTGA TCACGTTTTA GAGGCTCTGC TCAACACCTC TATGGACCTG 
GACTCCCTGA AGCCAGACTC TGTTCTGGAG GAGAAGTGGA TTAAAGAGGT GGTGACCAGG 
TCTGGGATGA AGTTCAGATG GGCCAAACTC AGCGAGAGCA GAGAGCGACC CAATAGAGAC 
GTCCAAAGGA GACCTCCGGC TGACCCAGCG CAGCTGGTGA GCCGCCCCCC GGTGGTCACC 
ATCATGGGTC ACGTGGACCA CGGGAAGACC ACGCTCCTGG ACAGCCTGAG GAAGAGCCAG 
ATCGTCTCCA CGGAGGCGGG AGGAATCACG CAGCACATCG GAGCCTTCCT CGTCCAGCTG 
CCTACGGGGG AGAAGATCAC ATTCCTGGAC ACGCCGGGCC ATGCCGCCTT CTCCTCCATG 
AGAGCCCGCG GAGCCAACGC CACCGACATA ATCATCCTGG TGGTGGCGGC AGACGACGGC 
GTCATGAACC AGACAGTCGA GTCCATCCAG CATGCCAGGA AAGCCAAAGT GCCTATCATC 
GTGGCGGTTA ACAAGTGTGA CAAACCTCAG GCCGACCCTC AGCGCGTCAA ACAGGAGCTG 
CTGGCTCATG ATGTCATCTG CGAGGAGTTT GGAGGAGACG TCCAGGCCAT CCACATCTCT 
GCTGTGAAGG GCGACAACCT GCTAGCCCTG GCCGAGGCCA CGGTGGCGTT GGCGGAGGTT 
CTGGAGCTGA AGGCTGACCC GAGCGGTCCG GTGGAGGCAG TCGTCATCGA GAGCCGAACG 
GACAAAGGCA AAGGACCGCT AACGACTGCC ATCGTCCAGA GGGGCACGCT GAGGCGAGGT 
TGCGTCCTGG TCGCCGGGAA GTGTTGGGCC AAAGTGCGTT TCCTGTTTGA TGAGAACGGG 
CAGGCTCTGG CGGAGGCGGC ACCGAGTACG GCGGTGCAGG TGGTCGGCTG GAAGGAGCTG 
CCATCCGCCG GGGAGACCAT CCTGGAGGTG GAGTCTGAGC AGCGAGCCAG AGAGGTGGTG 
GAGTGGAGGA GTCACGAGGA GGAGCAGCAG AAGCTCCTTG AGGAGCAGAG CGCCATCGAG 
CTGAAGCAGA AGCTCCACCT GGAGGAATAC CGACGGGAGC GGGAGGGGCT TGCCCACCTG 
AGCTGGCGGC AGAGGAAGGC CGCTCTGTAC CGGGCCAACA AGACCCGCTT CTCTATGAGG 
CCCAGCGAGA GGACGGAGAG CGACGAGCTC AGCCTGCCGC TCATCATCAA AGGAGATGTG 
GATGGGTCGG TCGAGGCGCT GCTCAACATC CTGGACGGCT ATGACGCCCA GGAGCAGTGC 
CAGCTGGAGG TGGTCCACTT CGGCATTGGA GATATCTCTG AGAACGACGT CAACATGGCG 
GAGATGTTTG GAGGTTCTAT CTACGGGTTC AACGTGGCGG CGAGCCGCTC CATCCAGCAG 
CTGGCGGCGA AGAAGGGCGT CCCGCTGCAG CTGCACTCCG TCATCTACAA ACTGATTGAT 
CAGCTGAAGG ACGAGCTGAG CAGCAAGCTG CCGGCCTGCG TGTCCCAGAA CAGCGTTGGT 
GAGGCGACGG TCCTCGCCAT CTTTGATGTC TCTGTGGGGA AGAAGAAGGT TCCGGTAGCT 
GGCTGCCGGG TCCAGAAGGG CCACCTGGAC CGACGCCTGA AATTCAGGCT GATCCGAGGA 
AAGGATACAG TGTGGGAGGG TTCTATATCG GCGCTGAAGC ACCACAAGGA CGACGTTCAG 
ACAGTGAAGA CGGGGATGGA GTGCGGCGTA TCAACAGACG GTGACGTGGA CTTCAAAGCC 
GGTGATGTCA TCGAGTGCTT TGAGGAGCTG GATGTTCCCC AGGTCACTTC CTGGGACCCG 
GGGTTCTGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_015226907.1
CDS	131..2239
Translation	MNVMSSVMRGARLLSHTDMHLLLCPSRSSAPLLTWTQSRRFASKQVKGQRKDPKKQNPKPKVDKQEVEIRQGMTAAALAAAMNKDFDHVLEALLNTSMDLDSLKPDSVLEEKWIKEVVTRSGMKFRWAKLSESRERPNRDVQRRPPADPAQLVSRPPVVTIMGHVDHGKTTLLDSLRKSQIVSTEAGGITQHIGAFLVQLPTGEKITFLDTPGHAAFSSMRARGANATDIIILVVAADDGVMNQTVESIQHARKAKVPIIVAVNKCDKPQADPQRVKQELLAHDVICEEFGGDVQAIHISAVKGDNLLALAEATVALAEVLELKADPSGPVEAVVIESRTDKGKGPLTTAIVQRGTLRRGCVLVAGKCWAKVRFLFDENGQALAEAAPSTAVQVVGWKELPSAGETILEVESEQRAREVVEWRSHEEEQQKLLEEQSAIELKQKLHLEEYRREREGLAHLSWRQRKAALYRANKTRFSMRPSERTESDELSLPLIIKGDVDGSVEALLNILDGYDAQEQCQLEVVHFGIGDISENDVNMAEMFGGSIYGFNVAASRSIQQLAAKKGVPLQLHSVIYKLIDQLKDELSSKLPACVSQNSVGEATVLAIFDVSVGKKKVPVAGCRVQKGHLDRRLKFRLIRGKDTVWEGSISALKHHKDDVQTVKTGMECGVSTDGDVDFKAGDVIECFEELDVPQVTSWDPGF

Target ORF information:

RefSeq Version	XM_015371421.1
Organism	Cyprinodon variegatus(sheepshead minnow)
Definition	Cyprinodon variegatus mitochondrial translational initiation factor 2 (mtif2), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_015371421.1

ORF Insert Sequence:

ATGAATGTGA TGTCATCAGT GATGCGGGGA GCAAGGCTGC TGTCCCACAC AGACATGCAC 
CTGCTCCTCT GTCCTTCCAG GAGCTCCGCC CCCCTGCTCA CCTGGACACA GAGCAGAAGG 
TTTGCCTCCA AACAGGTGAA AGGTCAGAGG AAGGACCCCA AGAAGCAGAA CCCAAAGCCC 
AAAGTGGACA AACAGGAAGT GGAGATCAGA CAGGGAATGA CGGCGGCAGC GCTAGCAGCA 
GCCATGAACA AAGACTTTGA TCACGTTTTA GAGGCTCTGC TCAACACCTC TATGGACCTG 
GACTCCCTGA AGCCAGACTC TGTTCTGGAG GAGAAGTGGA TTAAAGAGGT GGTGACCAGG 
TCTGGGATGA AGTTCAGATG GGCCAAACTC AGCGAGAGCA GAGAGCGACC CAATAGAGAC 
GTCCAAAGGA GACCTCCGGC TGACCCAGCG CAGCTGGTGA GCCGCCCCCC GGTGGTCACC 
ATCATGGGTC ACGTGGACCA CGGGAAGACC ACGCTCCTGG ACAGCCTGAG GAAGAGCCAG 
ATCGTCTCCA CGGAGGCGGG AGGAATCACG CAGCACATCG GAGCCTTCCT CGTCCAGCTG 
CCTACGGGGG AGAAGATCAC ATTCCTGGAC ACGCCGGGCC ATGCCGCCTT CTCCTCCATG 
AGAGCCCGCG GAGCCAACGC CACCGACATA ATCATCCTGG TGGTGGCGGC AGACGACGGC 
GTCATGAACC AGACAGTCGA GTCCATCCAG CATGCCAGGA AAGCCAAAGT GCCTATCATC 
GTGGCGGTTA ACAAGTGTGA CAAACCTCAG GCCGACCCTC AGCGCGTCAA ACAGGAGCTG 
CTGGCTCATG ATGTCATCTG CGAGGAGTTT GGAGGAGACG TCCAGGCCAT CCACATCTCT 
GCTGTGAAGG GCGACAACCT GCTAGCCCTG GCCGAGGCCA CGGTGGCGTT GGCGGAGGTT 
CTGGAGCTGA AGGCTGACCC GAGCGGTCCG GTGGAGGCAG TCGTCATCGA GAGCCGAACG 
GACAAAGGCA AAGGACCGCT AACGACTGCC ATCGTCCAGA GGGGCACGCT GAGGCGAGGT 
TGCGTCCTGG TCGCCGGGAA GTGTTGGGCC AAAGTGCGTT TCCTGTTTGA TGAGAACGGG 
CAGGCTCTGG CGGAGGCGGC ACCGAGTACG GCGGTGCAGG TGGTCGGCTG GAAGGAGCTG 
CCATCCGCCG GGGAGACCAT CCTGGAGGTG GAGTCTGAGC AGCGAGCCAG AGAGGTGGTG 
GAGTGGAGGA GTCACGAGGA GGAGCAGCAG AAGCTCCTTG AGGAGCAGAG CGCCATCGAG 
CTGAAGCAGA AGCTCCACCT GGAGGAATAC CGACGGGAGC GGGAGGGGCT TGCCCACCTG 
AGCTGGCGGC AGAGGAAGGC CGCTCTGTAC CGGGCCAACA AGACCCGCTT CTCTATGAGG 
CCCAGCGAGA GGACGGAGAG CGACGAGCTC AGCCTGCCGC TCATCATCAA AGGAGATGTG 
GATGGGTCGG TCGAGGCGCT GCTCAACATC CTGGACGGCT ATGACGCCCA GGAGCAGTGC 
CAGCTGGAGG TGGTCCACTT CGGCATTGGA GATATCTCTG AGAACGACGT CAACATGGCG 
GAGATGTTTG GAGGTTCTAT CTACGGGTTC AACGTGGCGG CGAGCCGCTC CATCCAGCAG 
CTGGCGGCGA AGAAGGGCGT CCCGCTGCAG CTGCACTCCG TCATCTACAA ACTGATTGAT 
CAGCTGAAGG ACGAGCTGAG CAGCAAGCTG CCGGCCTGCG TGTCCCAGAA CAGCGTTGGT 
GAGGCGACGG TCCTCGCCAT CTTTGATGTC TCTGTGGGGA AGAAGAAGGT TCCGGTAGCT 
GGCTGCCGGG TCCAGAAGGG CCACCTGGAC CGACGCCTGA AATTCAGGCT GATCCGAGGA 
AAGGATACAG TGTGGGAGGG TTCTATATCG GCGCTGAAGC ACCACAAGGA CGACGTTCAG 
ACAGTGAAGA CGGGGATGGA GTGCGGCGTA TCAACAGACG GTGACGTGGA CTTCAAAGCC 
GGTGATGTCA TCGAGTGCTT TGAGGAGCTG GATGTTCCCC AGGTCACTTC CTGGGACCCG 
GGGTTCTGA