SOS1 cDNA ORF clone, Cucumis sativus(cucumber)

Gene
Clones
gRNAs

Summary

Gene Symbol	SOS1
Entrez Gene ID	101209966
Full Name	son of sevenless homolog 1 (Drosophila)
Gene Type	protein-coding
Organism	Cucumis sativus(cucumber)

mRNA and Protein(s)

mRNA	Protein	Name
NM_001305732.1	NP_001292661.1	son of sevenless homolog 1
XM_031884949.1	XP_031740809.1	son of sevenless homolog 1 isoform X1

Pathways from BioSystems
Gene Ontology

The following SOS1 gene cDNA ORF clone sequences were retrieved from the NCBI Reference Sequence Database (RefSeq). These sequences represent the protein coding region of the SOS1 cDNA ORF which is encoded by the open reading frame (ORF) sequence. ORF sequences can be delivered in our standard vector, pcDNA3.1⁺/C-(K)DYK or the vector of your choice as an expression/transfection-ready ORF clone. Not the clone you want? Click here to find your clone.

***CloneID	Accession No.	Definition	**Vector	*Turnaround time	Price (USD)	Select
OCu149052	XM_031884949.1 Latest version!	Cucumis sativus son of sevenless homolog 1 (Drosophila) (SOS1), transcript variant X1, mRNA.	pcDNA3.1-C-(k)DYK or customized vector	14-16	$475.30 $679.00
OCu36617	NM_001305732.1 Latest version!	Cucumis sativus son of sevenless homolog 1 (Drosophila) (SOS1), mRNA.	pcDNA3.1-C-(k)DYK or customized vector	19-21	$629.30 $899.00

ORF Online Only Promotion

Next-day Shipping ORF Clones ( in default vector with tag)
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2-4 Clone	40% OFF
5 or more Clone	50% OFF
All Other ORF Clones
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** You may select a custom vector to replace pcDNA3.1+/C-(K)DYK after clone is added to cart.

** GenScript guarantees 100% sequence accuracy of all synthetic DNA constructs we deliver, but we do not guarantee protein expression in your experimental system. Protein expression is influenced by many factors that may vary between experiments or laboratories. In addition, please pay attention to the signal peptide, propeptide and transit peptide in target ORF, which may affect the choice of vector (N/C terminal tag vector).

***One clone ID might be correlated to multiple accession numbers, which share the same CDS sequence.

Reference Sequences (Refseq)

SOS1 ( XM_031884949.1 ) cDNA ORF clone, Cucumis sativus(cucumber) -> XP_031740809.1 Cucumis sativus son of sevenless homolog 1 (Drosophila) (SOS1), transcript variant X1, mRNA.

Price & Availability↑

CloneID	OCu149052
Clone ID Related Accession (Same CDS sequence)	XM_031884949.1
Accession Version	XM_031884949.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 2850bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-16
Organism	Cucumis sativus(cucumber)
Product	son of sevenless homolog 1 isoform X1
Comment	Comment: MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NC_026659.2) annotated using gene prediction method: Gnomon, supported by EST evidence. Also see: Documentation of NCBI's Annotation Process ##Genome-Annotation-Data-START## Annotation Provider :: NCBI Annotation Status :: Full annotation Annotation Name :: Cucumis sativus Annotation Release 102 Annotation Version :: 102 Annotation Pipeline :: NCBI eukaryotic genome annotation pipeline Annotation Software Version :: 8.3 Annotation Method :: Best-placed RefSeq; Gnomon Features Annotated :: Gene; mRNA; CDS; ncRNA ##Genome-Annotation-Data-END##

ATGATGGCAA TTGTGGTTTA TCAACTTTTT TATCAAATGG TCCTTGGAAA GAGCTTTAAC 
TGGGGTGCTA TAATCAAATA TCTCACACAA GTCTCGCTGG GAGCTTTGGG AATTGGTCTT 
GCTTTTGGGA TTGCATCTGT TTTATGGCTC GGGTTCATTT TCAACGACAC AGTGATAGAG 
ATTGCATTAA CACTAGCTGT GAGCTATATT GCTTACTTCA CTGCTCAAGA GGGAGCTGAT 
GTTTCGGGTG TTCTGACAGT GATGAGTTTA GGAATGTTTT ATGCTGCAGT TGCAAGAACA 
GCTTTTAAGG GTGACGGTCA ACAAAGCTTG CATCATTTTT GGGAAATGGT TGCTTACATA 
GCGAATACAT TAATTTTCAT ATTGAGTGGA GTTGTTATCG CTGAAGGTGT TCTTGGAAGC 
GAAGGAATTC TTGATAATGG AGCTTCATGG GGCTACCTCA TTATACTTTA TGTGTACGTG 
CAAGCTTCTC GATTTATGGT TGTTGGAGTA TTATATCCAT TTCTGCGGTA TTTTGGGTAT 
GGCTTGGATT GGAAAGAAGC TACAATACTA ATATGGTCAG GTCTGCGTGG AGCTGTTGCT 
CTGTCACTTT CACTGTCAGT TAAGCGATCA AGTGACCAAT CATTGTACAT CAGTTCAGAA 
ACTGGAACAC TGTTTGTTTT TTTCACGGGT GGAATTGTAT TCTTGACGCT GATTGTAAAT 
GGTTCGACCA CACAATTCAT TTTACATCTT CTCAACATGG ACAAGCTCTC AGTGGCTAAG 
AAACGCATAT TGGATTATAC AAAATACGAG ATGATGAACA AAGCATTGGG CGCCTTTGGA 
GATCTTGGAG ATGATGAGGA ATTGGGACCT GCTGACTGGG CTACAGTTAA AAGACATATT 
ACAAGTCTCA GTCATGTGGA GGGGGAGCCA CTGCACCCGC ACAATGCATT TGAAAGTGAT 
CAAAATGTTC GATCTATGAA TTTGAGAGAC ATTCGTCTGC GACTTTTGAA TGGTGTTCAA 
GCTGCTTACT GGGGTATGCT TGATGAGGGA AGGATAACAC AAAGTACTGC CAATATCTTG 
ATGCAATCTG TGGATGAAGC CCTTGATCAG ATTGCATATG AGCCCCTTTG TGATTGGAAG 
GGTTTAAAGT CTAATGTCCA TTTTCCAAAC TATTACAAGT TCCTTCAGAC AAGCGTTTTC 
CCTCAGAAGT TGGTTACATA TTTCACTGTG GAAAGATTAG AGTCCGGGTG TTACATTTGT 
GCTGCATTTC TCCGTGCACA TCGAATTGCA AGACAGCAGC TTCATGAGTT TATAGGTGAT 
AGTGATATTG CTTCTACTGT AATTAATGAA AGTGAAGCTG AGGGTGAAGA AGCACGAAAG 
TTTCTAGAAG ATGTTCGTGA GACATTTCCT CAGGTTTTGC GTGTTGTCAA AACCAGACAA 
GTAACATATT CAGTGCTCAA CCATTTAATT GAATATGTCC AAAATCTGGA GAAGGTTGGC 
CTGTTAGAAG AGAAAGAGAT GTTGCATCTT CATGATGCTG TTCAGACCGA CTTGAAAAGG 
CTTCTACGAA ATCCTCCTTT GTTAAAGATT CCAAAAATGC GGAACTTAAT CAGTGCCCAT 
CCATTTCTTG GGGCACTTCC TCCTTTAGTG CGTGAACCAC TTGAAGTTTC AACAAAAGAA 
GTGATGAAAC TACGTGGTGT AACACTTTAC AAGGAAGGTT CAAAGCCTAG TGGTGTTTGG 
CTTATATCCA ACGGAGTGGT AAAGTGGATA AGTAAGAGCA TGAGAAACAA GTTCTCCTTG 
CACCCCACAT TTACTCATGG AAGCACTTTG GGCCTATATG AATTGCTTAC TGGGAAGCCA 
TGCTTCTGTG ACATGATTAC AGATTCCGTT GTTCTCTCTT TTTTTATTGA ACATGACAAG 
TTTCTTTCAA TACTTAGATC AGATCCTTCG GTTGAAGACT TCTTGTGGCA GGAAAGTTCC 
ATTGTTCTTG CCAAACTTTT GCTTCCACAA GTATTTGAGA AAATGGAAAT GAGAGATCTG 
AGAGTTCTTG TTGTTGAAAG GTCAGTAATG ACTACGCATA TAGCAGGAGA AACAATAGAA 
ATTCCACCCC ATTCTATTGG CCTTTTGTTG GAAGGATTTA TCAAAAGTCA TGGCATCCAA 
GAGGAACTTA TAGCATCACC TGCTGTATTG TTCTCCTCGC ACAGGAATCC GAGCTTCCAA 
AATATGGAAA ACTCAGGGAT GAGTGGTTCT AGTTTTTCTC ATCAAGGCTC CCATTATGAA 
GTTGAAACAA GATCAAGAGT GATCGTTTTT GACATGGGTG CCCTTCAATC AGAAGAAAAT 
CTAAATCGGT CGTCGTCTTT TATTCATTCT GTTGATCACC CTCAAAGATC TCTAAGCAGA 
GATCACTCAG GTCTTATGAG TTGGCCTGAA ATGCTTTCTA AGCCTAGACC ACCACAGAAG 
CAGAAATCTG AAAGAATTGA GCGTCCAGCT GATAGCTTGT CTGCAAAGGC CATGCAACTA 
AGCATATACG GTAGCATGGT TGATTTTCGT CAGCGGACTA AGAGTTTTCC GGGCAATATT 
GCGGAACCAT CTCATAGCCG GTCGAATCCT GCAATTGGTT CACATAAAGG TGTTTCCCTT 
CCATACGTAA AATCAGAAGG AGCTGCTACA TTGAAGAAGA GGCTTGATGC AAGAAAGTTA 
CCAATAAGCA ATGTTAGACC CCCACAACAA AAAGCACTTC CTAATGAGAG AAATGTAAGG 
GATGATTCAA GTGAGGAATC GGGTGGTGAG GACGATGTGA TAGTGAGAAT TGATTCACCA 
AGTGTGCTTT CGTTTCACCA GGTACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	XP_031740809.1
CDS	136..2985
Translation	MMAIVVYQLFYQMVLGKSFNWGAIIKYLTQVSLGALGIGLAFGIASVLWLGFIFNDTVIEIALTLAVSYIAYFTAQEGADVSGVLTVMSLGMFYAAVARTAFKGDGQQSLHHFWEMVAYIANTLIFILSGVVIAEGVLGSEGILDNGASWGYLIILYVYVQASRFMVVGVLYPFLRYFGYGLDWKEATILIWSGLRGAVALSLSLSVKRSSDQSLYISSETGTLFVFFTGGIVFLTLIVNGSTTQFILHLLNMDKLSVAKKRILDYTKYEMMNKALGAFGDLGDDEELGPADWATVKRHITSLSHVEGEPLHPHNAFESDQNVRSMNLRDIRLRLLNGVQAAYWGMLDEGRITQSTANILMQSVDEALDQIAYEPLCDWKGLKSNVHFPNYYKFLQTSVFPQKLVTYFTVERLESGCYICAAFLRAHRIARQQLHEFIGDSDIASTVINESEAEGEEARKFLEDVRETFPQVLRVVKTRQVTYSVLNHLIEYVQNLEKVGLLEEKEMLHLHDAVQTDLKRLLRNPPLLKIPKMRNLISAHPFLGALPPLVREPLEVSTKEVMKLRGVTLYKEGSKPSGVWLISNGVVKWISKSMRNKFSLHPTFTHGSTLGLYELLTGKPCFCDMITDSVVLSFFIEHDKFLSILRSDPSVEDFLWQESSIVLAKLLLPQVFEKMEMRDLRVLVVERSVMTTHIAGETIEIPPHSIGLLLEGFIKSHGIQEELIASPAVLFSSHRNPSFQNMENSGMSGSSFSHQGSHYEVETRSRVIVFDMGALQSEENLNRSSSFIHSVDHPQRSLSRDHSGLMSWPEMLSKPRPPQKQKSERIERPADSLSAKAMQLSIYGSMVDFRQRTKSFPGNIAEPSHSRSNPAIGSHKGVSLPYVKSEGAATLKKRLDARKLPISNVRPPQQKALPNERNVRDDSSEESGGEDDVIVRIDSPSVLSFHQVP

Target ORF information:

RefSeq Version	XM_031884949.1
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus son of sevenless homolog 1 (Drosophila) (SOS1), transcript variant X1, mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

XM_031884949.1

ORF Insert Sequence:

ATGATGGCAA TTGTGGTTTA TCAACTTTTT TATCAAATGG TCCTTGGAAA GAGCTTTAAC 
TGGGGTGCTA TAATCAAATA TCTCACACAA GTCTCGCTGG GAGCTTTGGG AATTGGTCTT 
GCTTTTGGGA TTGCATCTGT TTTATGGCTC GGGTTCATTT TCAACGACAC AGTGATAGAG 
ATTGCATTAA CACTAGCTGT GAGCTATATT GCTTACTTCA CTGCTCAAGA GGGAGCTGAT 
GTTTCGGGTG TTCTGACAGT GATGAGTTTA GGAATGTTTT ATGCTGCAGT TGCAAGAACA 
GCTTTTAAGG GTGACGGTCA ACAAAGCTTG CATCATTTTT GGGAAATGGT TGCTTACATA 
GCGAATACAT TAATTTTCAT ATTGAGTGGA GTTGTTATCG CTGAAGGTGT TCTTGGAAGC 
GAAGGAATTC TTGATAATGG AGCTTCATGG GGCTACCTCA TTATACTTTA TGTGTACGTG 
CAAGCTTCTC GATTTATGGT TGTTGGAGTA TTATATCCAT TTCTGCGGTA TTTTGGGTAT 
GGCTTGGATT GGAAAGAAGC TACAATACTA ATATGGTCAG GTCTGCGTGG AGCTGTTGCT 
CTGTCACTTT CACTGTCAGT TAAGCGATCA AGTGACCAAT CATTGTACAT CAGTTCAGAA 
ACTGGAACAC TGTTTGTTTT TTTCACGGGT GGAATTGTAT TCTTGACGCT GATTGTAAAT 
GGTTCGACCA CACAATTCAT TTTACATCTT CTCAACATGG ACAAGCTCTC AGTGGCTAAG 
AAACGCATAT TGGATTATAC AAAATACGAG ATGATGAACA AAGCATTGGG CGCCTTTGGA 
GATCTTGGAG ATGATGAGGA ATTGGGACCT GCTGACTGGG CTACAGTTAA AAGACATATT 
ACAAGTCTCA GTCATGTGGA GGGGGAGCCA CTGCACCCGC ACAATGCATT TGAAAGTGAT 
CAAAATGTTC GATCTATGAA TTTGAGAGAC ATTCGTCTGC GACTTTTGAA TGGTGTTCAA 
GCTGCTTACT GGGGTATGCT TGATGAGGGA AGGATAACAC AAAGTACTGC CAATATCTTG 
ATGCAATCTG TGGATGAAGC CCTTGATCAG ATTGCATATG AGCCCCTTTG TGATTGGAAG 
GGTTTAAAGT CTAATGTCCA TTTTCCAAAC TATTACAAGT TCCTTCAGAC AAGCGTTTTC 
CCTCAGAAGT TGGTTACATA TTTCACTGTG GAAAGATTAG AGTCCGGGTG TTACATTTGT 
GCTGCATTTC TCCGTGCACA TCGAATTGCA AGACAGCAGC TTCATGAGTT TATAGGTGAT 
AGTGATATTG CTTCTACTGT AATTAATGAA AGTGAAGCTG AGGGTGAAGA AGCACGAAAG 
TTTCTAGAAG ATGTTCGTGA GACATTTCCT CAGGTTTTGC GTGTTGTCAA AACCAGACAA 
GTAACATATT CAGTGCTCAA CCATTTAATT GAATATGTCC AAAATCTGGA GAAGGTTGGC 
CTGTTAGAAG AGAAAGAGAT GTTGCATCTT CATGATGCTG TTCAGACCGA CTTGAAAAGG 
CTTCTACGAA ATCCTCCTTT GTTAAAGATT CCAAAAATGC GGAACTTAAT CAGTGCCCAT 
CCATTTCTTG GGGCACTTCC TCCTTTAGTG CGTGAACCAC TTGAAGTTTC AACAAAAGAA 
GTGATGAAAC TACGTGGTGT AACACTTTAC AAGGAAGGTT CAAAGCCTAG TGGTGTTTGG 
CTTATATCCA ACGGAGTGGT AAAGTGGATA AGTAAGAGCA TGAGAAACAA GTTCTCCTTG 
CACCCCACAT TTACTCATGG AAGCACTTTG GGCCTATATG AATTGCTTAC TGGGAAGCCA 
TGCTTCTGTG ACATGATTAC AGATTCCGTT GTTCTCTCTT TTTTTATTGA ACATGACAAG 
TTTCTTTCAA TACTTAGATC AGATCCTTCG GTTGAAGACT TCTTGTGGCA GGAAAGTTCC 
ATTGTTCTTG CCAAACTTTT GCTTCCACAA GTATTTGAGA AAATGGAAAT GAGAGATCTG 
AGAGTTCTTG TTGTTGAAAG GTCAGTAATG ACTACGCATA TAGCAGGAGA AACAATAGAA 
ATTCCACCCC ATTCTATTGG CCTTTTGTTG GAAGGATTTA TCAAAAGTCA TGGCATCCAA 
GAGGAACTTA TAGCATCACC TGCTGTATTG TTCTCCTCGC ACAGGAATCC GAGCTTCCAA 
AATATGGAAA ACTCAGGGAT GAGTGGTTCT AGTTTTTCTC ATCAAGGCTC CCATTATGAA 
GTTGAAACAA GATCAAGAGT GATCGTTTTT GACATGGGTG CCCTTCAATC AGAAGAAAAT 
CTAAATCGGT CGTCGTCTTT TATTCATTCT GTTGATCACC CTCAAAGATC TCTAAGCAGA 
GATCACTCAG GTCTTATGAG TTGGCCTGAA ATGCTTTCTA AGCCTAGACC ACCACAGAAG 
CAGAAATCTG AAAGAATTGA GCGTCCAGCT GATAGCTTGT CTGCAAAGGC CATGCAACTA 
AGCATATACG GTAGCATGGT TGATTTTCGT CAGCGGACTA AGAGTTTTCC GGGCAATATT 
GCGGAACCAT CTCATAGCCG GTCGAATCCT GCAATTGGTT CACATAAAGG TGTTTCCCTT 
CCATACGTAA AATCAGAAGG AGCTGCTACA TTGAAGAAGA GGCTTGATGC AAGAAAGTTA 
CCAATAAGCA ATGTTAGACC CCCACAACAA AAAGCACTTC CTAATGAGAG AAATGTAAGG 
GATGATTCAA GTGAGGAATC GGGTGGTGAG GACGATGTGA TAGTGAGAAT TGATTCACCA 
AGTGTGCTTT CGTTTCACCA GGTACCATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

SOS1 ( NM_001305732.1 ) cDNA ORF clone, Cucumis sativus(cucumber) -> NP_001292661.1 Cucumis sativus son of sevenless homolog 1 (Drosophila) (SOS1), mRNA.

Price & Availability↑

CloneID	OCu36617
Clone ID Related Accession (Same CDS sequence)	NM_001305732.1
Accession Version	NM_001305732.1 Latest version!	Documents for ORF clone product in default vector
Sequence Information	ORF Nucleotide Sequence (Length: 3435bp) Protein sequence SNP
Vector	pcDNA3.1-C-(k)DYK or customized vector	User Manual
Clone information	Clone Map	MSDS
Tag on pcDNA3.1⁺/C-(K)DYK	C terminal DYKDDDDK tags
ORF Insert Method	CloneEZ™ Seamless cloning technology
Insert Structure	linear
Update Date	2019-12-21
Organism	Cucumis sativus(cucumber)
Product	son of sevenless homolog 1
Comment	Comment: PROVISIONAL REFSEQ: This record has not yet been subject to final NCBI review. The reference sequence was derived from JQ655747.1. On Mar 30, 2015 this sequence version replaced XM_004150107.2. ##Evidence-Data-START## Transcript exon combination :: JQ655747.1 [ECO:0000332] RNAseq introns :: single sample supports all introns SAMN02570546, SAMN02570552 [ECO:0000348] ##Evidence-Data-END##

ATGGCGGGAG TAGAATTGCT GGATATGTTT CCTCATCGGG ACCTTGAAGA GGGAGCCACT 
GCCTCTTCTG ATTGGAATCC AACTGATGCT GTGCTGTTTT TTGGACTGAG TTTGGTACTT 
GGAATTGCTT GCAGACACCT ATTGCGAGGC ACTAGAGTTC CTTATACAGT TGCCTTGCTT 
GTTCTTGGCA TAGTTCTTGG ATCTATTGAA TATGGCACAC ATCATCAATT GGGAAAGATA 
GGGGATGGCA TACGTCTCTG GGCAAAAATT GATCCTGATT TATTACTGGC TGTTTTCCTC 
CCTGCTTTGC TGTTCGAGAG TTCCTTTTCA ATGGAAGTGC ACCAAATAAA GAGGTGTCTG 
GCCCAAATGA TCTTATTGGC TGGTCCAGGT GTTTTAATTT CAACCTTCTT GCTAGGATCT 
GCTTTCAAGC TCACTTTCCC GTATAATTGG AGTTGGAAAA CATCATTGTT GCTCGGGGGA 
CTTCTGATTG CCACAGATCC TGTGGCTGTT GTGGCTCTAT TGAAAGAGCT AGGAGCAAGT 
AAAAAACTGA ATACAATAAT AGAAGGAGAA TCCTTGATGA ATGATGGGAC AGCAATTGTG 
GTTTATCAAC TTTTTTATCA AATGGTCCTT GGAAAGAGCT TTAACTGGGG TGCTATAATC 
AAATATCTCA CACAAGTCTC GCTGGGAGCT TTGGGAATTG GTCTTGCTTT TGGGATTGCA 
TCTGTTTTAT GGCTCGGGTT CATTTTCAAC GACACAGTGA TAGAGATTGC ATTAACACTA 
GCTGTGAGCT ATATTGCTTA CTTCACTGCT CAAGAGGGAG CTGATGTTTC GGGTGTTCTG 
ACAGTGATGA GTTTAGGAAT GTTTTATGCT GCAGTTGCAA GAACAGCTTT TAAGGGTGAC 
GGTCAACAAA GCTTGCATCA TTTTTGGGAA ATGGTTGCTT ACATAGCGAA TACATTAATT 
TTCATATTGA GTGGAGTTGT TATCGCTGAA GGTGTTCTTG GAAGCGAAGG AATTCTTGAT 
AATGGAGCTT CATGGGGCTA CCTCATTATA CTTTATGTGT ACGTGCAAGC TTCTCGATTT 
ATGGTTGTTG GAGTATTATA TCCATTTCTG CGGTATTTTG GGTATGGCTT GGATTGGAAA 
GAAGCTACAA TACTAATATG GTCAGGTCTG CGTGGAGCTG TTGCTCTGTC ACTTTCACTG 
TCAGTTAAGC GATCAAGTGA CCAATCATTG TACATCAGTT CAGAAACTGG AACACTGTTT 
GTTTTTTTCA CGGGTGGAAT TGTATTCTTG ACGCTGATTG TAAATGGTTC GACCACACAA 
TTCATTTTAC ATCTTCTCAA CATGGACAAG CTCTCAGTGG CTAAGAAACG CATATTGGAT 
TATACAAAAT ACGAGATGAT GAACAAAGCA TTGGGCGCCT TTGGAGATCT TGGAGATGAT 
GAGGAATTGG GACCTGCTGA CTGGGCTACA GTTAAAAGAC ATATTACAAG TCTCAGTCAT 
GTGGAGGGGG AGCCACTGCA CCCGCACAAT GCATTTGAAA GTGATCAAAA TGTTCGATCT 
ATGAATTTGA GAGACATTCG TCTGCGACTT TTGAATGGTG TTCAAGCTGC TTACTGGGGT 
ATGCTTGATG AGGGAAGGAT AACACAAAGT ACTGCCAATA TCTTGATGCA ATCTGTGGAT 
GAAGCCCTTG ATCAGATTGC ATATGAGCCC CTTTGTGATT GGAAGGGTTT AAAGTCTAAT 
GTCCATTTTC CAAACTATTA CAAGTTCCTT CAGACAAGCG TTTTCCCTCA GAAGTTGGTT 
ACATATTTCA CTGTGGAAAG ATTAGAGTCC GGGTGTTACA TTTGTGCTGC ATTTCTCCGT 
GCACATCGAA TTGCAAGACA GCAGCTTCAT GAGTTTATAG GTGATAGTGA TATTGCTTCT 
ACTGTAATTA GTGAAAGTGA AGCTGAGGGT GAAGAAGCAC GAAAGTTTCT AGAAGATGTT 
CGTGAGACAT TTCCTCAGGT TTTGCGTGTT GTCAAAACCA GACAAGTAAC ATATTCAGTG 
CTCAACCATT TAATTGAATA TGTCCAAAAT CTGGAGAAGG TTGGCCTGTT AGAAGAGAAA 
GAGATGTTGC ATCTTCATGA TGCTGTTCAG ACCGACTTGA AAAGGCTTCT ACGAAATCCT 
CCTTTGTTAA AGATTCCAAA AATGCGGAAC TTAATCAGTG CCCATCCATT TCTTGGGGCA 
CTTCCTCCTT TAGTGCGTGA ACCACTTGAA GTTTCAACAA AAGAAGTGAT GAAACTACGT 
GGTGTAACAC TTTACAAGGA AGGTTCAAAG CCTAGTGGTG TTTGGCTTAT ATCCAACGGA 
GTGGTAAAGT GGATAAGTAA GAGCATGAGA AACAAGTTCT CCTTGCACCC CACATTTACT 
CATGGAAGCA CTTTGGGCCT ATATGAATTG CTTACTGGGA AGCCATGCTT CTGTGACATG 
ATTACAGATT CCGTTGTTCT CTCTTTTTTT ATTGAACATG ACAAGTTTCT TTCAATACTT 
AGATCAGATC CTTCGGTTGA AGACTTCTTG TGGCAGGAAA GTTCCATTGT TCTTGCCAAA 
CTTTTGCTTC CACAAGTATT TGAGAAAATG GAAATGAGAG ATCTGAGAGT TCTTGTTGTT 
GAAAGGTCAG TAATGACTAC GCATATAGCA GGAGAAACAA TAGAAATTCC ACCCCATTCT 
ATTGGCCTTT TGTTGGAAGG ATTTATCAAA AGTCATGGCA TCCAAGAGGA ACTTATAGCA 
TCACCCGCTG TATTGTTCTC CTCGCACAGG AATCCGAGCT TCCAAAATAT GGAAAACTCA 
GGGATGAGTG GTTCTAGTTT TTCTCATCAA GGCTCCCATT ATGAAGTTGA AACAAGATCA 
AGAGTGATCG TTTTTGACAT GGGTGCCCTT CAATCAGAAG AAAATCTAAA TCGGTCGTCG 
TCTTTTATTC ATTCTGTTGA TCACCCTCAA AGATCTCTAA GCAGAGATCA CTCAGGTCTT 
ATGAGTTGGC CTGAAATGCT TTCTAAGCCT AGACCACCAC AGAAGCAGAA ATCTGAAAGA 
ATTGAGCGTC CAGCTGATAG CTTGTCTGCA AAGGCCATGC AACTAAGCAT ATACGGTAGC 
ATGGTTGATT TTCGTCAGCG GACTAAGAGT TTTCCGGGCA ATATTGCGGA ACCATCTCAT 
AGCCGGTCGA ATCCTGCAAT TGGTTCACAT AAAGGTGTTT CCCTTCCATA CGTAAAATCA 
GAAGGAGCTG CTACATTGAA GAAGAGGCTT GATGCAAGAA AGTTACCAAT AAGCAATGTT 
AGACCCCCAC AACAAAAAGC ACTTCCTAAT GAGAGAAATG TAAGGGATGA TTCAAGTGAG 
GAATCGGGTG GTGAGGACGA TGTGATAGTG AGAATTGATT CACCAAGTGT GCTTTCGTTT 
CACCAGGTAC CATGA

The stop codons will be deleted if pcDNA3.1⁺/C-(K)DYK vector is selected.

RefSeq	NP_001292661.1
CDS	66..3500
Translation	MAGVELLDMFPHRDLEEGATASSDWNPTDAVLFFGLSLVLGIACRHLLRGTRVPYTVALLVLGIVLGSIEYGTHHQLGKIGDGIRLWAKIDPDLLLAVFLPALLFESSFSMEVHQIKRCLAQMILLAGPGVLISTFLLGSAFKLTFPYNWSWKTSLLLGGLLIATDPVAVVALLKELGASKKLNTIIEGESLMNDGTAIVVYQLFYQMVLGKSFNWGAIIKYLTQVSLGALGIGLAFGIASVLWLGFIFNDTVIEIALTLAVSYIAYFTAQEGADVSGVLTVMSLGMFYAAVARTAFKGDGQQSLHHFWEMVAYIANTLIFILSGVVIAEGVLGSEGILDNGASWGYLIILYVYVQASRFMVVGVLYPFLRYFGYGLDWKEATILIWSGLRGAVALSLSLSVKRSSDQSLYISSETGTLFVFFTGGIVFLTLIVNGSTTQFILHLLNMDKLSVAKKRILDYTKYEMMNKALGAFGDLGDDEELGPADWATVKRHITSLSHVEGEPLHPHNAFESDQNVRSMNLRDIRLRLLNGVQAAYWGMLDEGRITQSTANILMQSVDEALDQIAYEPLCDWKGLKSNVHFPNYYKFLQTSVFPQKLVTYFTVERLESGCYICAAFLRAHRIARQQLHEFIGDSDIASTVISESEAEGEEARKFLEDVRETFPQVLRVVKTRQVTYSVLNHLIEYVQNLEKVGLLEEKEMLHLHDAVQTDLKRLLRNPPLLKIPKMRNLISAHPFLGALPPLVREPLEVSTKEVMKLRGVTLYKEGSKPSGVWLISNGVVKWISKSMRNKFSLHPTFTHGSTLGLYELLTGKPCFCDMITDSVVLSFFIEHDKFLSILRSDPSVEDFLWQESSIVLAKLLLPQVFEKMEMRDLRVLVVERSVMTTHIAGETIEIPPHSIGLLLEGFIKSHGIQEELIASPAVLFSSHRNPSFQNMENSGMSGSSFSHQGSHYEVETRSRVIVFDMGALQSEENLNRSSSFIHSVDHPQRSLSRDHSGLMSWPEMLSKPRPPQKQKSERIERPADSLSAKAMQLSIYGSMVDFRQRTKSFPGNIAEPSHSRSNPAIGSHKGVSLPYVKSEGAATLKKRLDARKLPISNVRPPQQKALPNERNVRDDSSEESGGEDDVIVRIDSPSVLSFHQVP

Target ORF information:

RefSeq Version	NM_001305732.1
Organism	Cucumis sativus(cucumber)
Definition	Cucumis sativus son of sevenless homolog 1 (Drosophila) (SOS1), mRNA.

Target ORF information:

Epitope	DYKDDDDK
Bacterial selection	AMP^R
Mammalian selection	Neo^R
Vector	pcDNA3.1⁺/C-(K)DYK

NM_001305732.1

ORF Insert Sequence:

ATGGCGGGAG TAGAATTGCT GGATATGTTT CCTCATCGGG ACCTTGAAGA GGGAGCCACT 
GCCTCTTCTG ATTGGAATCC AACTGATGCT GTGCTGTTTT TTGGACTGAG TTTGGTACTT 
GGAATTGCTT GCAGACACCT ATTGCGAGGC ACTAGAGTTC CTTATACAGT TGCCTTGCTT 
GTTCTTGGCA TAGTTCTTGG ATCTATTGAA TATGGCACAC ATCATCAATT GGGAAAGATA 
GGGGATGGCA TACGTCTCTG GGCAAAAATT GATCCTGATT TATTACTGGC TGTTTTCCTC 
CCTGCTTTGC TGTTCGAGAG TTCCTTTTCA ATGGAAGTGC ACCAAATAAA GAGGTGTCTG 
GCCCAAATGA TCTTATTGGC TGGTCCAGGT GTTTTAATTT CAACCTTCTT GCTAGGATCT 
GCTTTCAAGC TCACTTTCCC GTATAATTGG AGTTGGAAAA CATCATTGTT GCTCGGGGGA 
CTTCTGATTG CCACAGATCC TGTGGCTGTT GTGGCTCTAT TGAAAGAGCT AGGAGCAAGT 
AAAAAACTGA ATACAATAAT AGAAGGAGAA TCCTTGATGA ATGATGGGAC AGCAATTGTG 
GTTTATCAAC TTTTTTATCA AATGGTCCTT GGAAAGAGCT TTAACTGGGG TGCTATAATC 
AAATATCTCA CACAAGTCTC GCTGGGAGCT TTGGGAATTG GTCTTGCTTT TGGGATTGCA 
TCTGTTTTAT GGCTCGGGTT CATTTTCAAC GACACAGTGA TAGAGATTGC ATTAACACTA 
GCTGTGAGCT ATATTGCTTA CTTCACTGCT CAAGAGGGAG CTGATGTTTC GGGTGTTCTG 
ACAGTGATGA GTTTAGGAAT GTTTTATGCT GCAGTTGCAA GAACAGCTTT TAAGGGTGAC 
GGTCAACAAA GCTTGCATCA TTTTTGGGAA ATGGTTGCTT ACATAGCGAA TACATTAATT 
TTCATATTGA GTGGAGTTGT TATCGCTGAA GGTGTTCTTG GAAGCGAAGG AATTCTTGAT 
AATGGAGCTT CATGGGGCTA CCTCATTATA CTTTATGTGT ACGTGCAAGC TTCTCGATTT 
ATGGTTGTTG GAGTATTATA TCCATTTCTG CGGTATTTTG GGTATGGCTT GGATTGGAAA 
GAAGCTACAA TACTAATATG GTCAGGTCTG CGTGGAGCTG TTGCTCTGTC ACTTTCACTG 
TCAGTTAAGC GATCAAGTGA CCAATCATTG TACATCAGTT CAGAAACTGG AACACTGTTT 
GTTTTTTTCA CGGGTGGAAT TGTATTCTTG ACGCTGATTG TAAATGGTTC GACCACACAA 
TTCATTTTAC ATCTTCTCAA CATGGACAAG CTCTCAGTGG CTAAGAAACG CATATTGGAT 
TATACAAAAT ACGAGATGAT GAACAAAGCA TTGGGCGCCT TTGGAGATCT TGGAGATGAT 
GAGGAATTGG GACCTGCTGA CTGGGCTACA GTTAAAAGAC ATATTACAAG TCTCAGTCAT 
GTGGAGGGGG AGCCACTGCA CCCGCACAAT GCATTTGAAA GTGATCAAAA TGTTCGATCT 
ATGAATTTGA GAGACATTCG TCTGCGACTT TTGAATGGTG TTCAAGCTGC TTACTGGGGT 
ATGCTTGATG AGGGAAGGAT AACACAAAGT ACTGCCAATA TCTTGATGCA ATCTGTGGAT 
GAAGCCCTTG ATCAGATTGC ATATGAGCCC CTTTGTGATT GGAAGGGTTT AAAGTCTAAT 
GTCCATTTTC CAAACTATTA CAAGTTCCTT CAGACAAGCG TTTTCCCTCA GAAGTTGGTT 
ACATATTTCA CTGTGGAAAG ATTAGAGTCC GGGTGTTACA TTTGTGCTGC ATTTCTCCGT 
GCACATCGAA TTGCAAGACA GCAGCTTCAT GAGTTTATAG GTGATAGTGA TATTGCTTCT 
ACTGTAATTA GTGAAAGTGA AGCTGAGGGT GAAGAAGCAC GAAAGTTTCT AGAAGATGTT 
CGTGAGACAT TTCCTCAGGT TTTGCGTGTT GTCAAAACCA GACAAGTAAC ATATTCAGTG 
CTCAACCATT TAATTGAATA TGTCCAAAAT CTGGAGAAGG TTGGCCTGTT AGAAGAGAAA 
GAGATGTTGC ATCTTCATGA TGCTGTTCAG ACCGACTTGA AAAGGCTTCT ACGAAATCCT 
CCTTTGTTAA AGATTCCAAA AATGCGGAAC TTAATCAGTG CCCATCCATT TCTTGGGGCA 
CTTCCTCCTT TAGTGCGTGA ACCACTTGAA GTTTCAACAA AAGAAGTGAT GAAACTACGT 
GGTGTAACAC TTTACAAGGA AGGTTCAAAG CCTAGTGGTG TTTGGCTTAT ATCCAACGGA 
GTGGTAAAGT GGATAAGTAA GAGCATGAGA AACAAGTTCT CCTTGCACCC CACATTTACT 
CATGGAAGCA CTTTGGGCCT ATATGAATTG CTTACTGGGA AGCCATGCTT CTGTGACATG 
ATTACAGATT CCGTTGTTCT CTCTTTTTTT ATTGAACATG ACAAGTTTCT TTCAATACTT 
AGATCAGATC CTTCGGTTGA AGACTTCTTG TGGCAGGAAA GTTCCATTGT TCTTGCCAAA 
CTTTTGCTTC CACAAGTATT TGAGAAAATG GAAATGAGAG ATCTGAGAGT TCTTGTTGTT 
GAAAGGTCAG TAATGACTAC GCATATAGCA GGAGAAACAA TAGAAATTCC ACCCCATTCT 
ATTGGCCTTT TGTTGGAAGG ATTTATCAAA AGTCATGGCA TCCAAGAGGA ACTTATAGCA 
TCACCCGCTG TATTGTTCTC CTCGCACAGG AATCCGAGCT TCCAAAATAT GGAAAACTCA 
GGGATGAGTG GTTCTAGTTT TTCTCATCAA GGCTCCCATT ATGAAGTTGA AACAAGATCA 
AGAGTGATCG TTTTTGACAT GGGTGCCCTT CAATCAGAAG AAAATCTAAA TCGGTCGTCG 
TCTTTTATTC ATTCTGTTGA TCACCCTCAA AGATCTCTAA GCAGAGATCA CTCAGGTCTT 
ATGAGTTGGC CTGAAATGCT TTCTAAGCCT AGACCACCAC AGAAGCAGAA ATCTGAAAGA 
ATTGAGCGTC CAGCTGATAG CTTGTCTGCA AAGGCCATGC AACTAAGCAT ATACGGTAGC 
ATGGTTGATT TTCGTCAGCG GACTAAGAGT TTTCCGGGCA ATATTGCGGA ACCATCTCAT 
AGCCGGTCGA ATCCTGCAAT TGGTTCACAT AAAGGTGTTT CCCTTCCATA CGTAAAATCA 
GAAGGAGCTG CTACATTGAA GAAGAGGCTT GATGCAAGAA AGTTACCAAT AAGCAATGTT 
AGACCCCCAC AACAAAAAGC ACTTCCTAAT GAGAGAAATG TAAGGGATGA TTCAAGTGAG 
GAATCGGGTG GTGAGGACGA TGTGATAGTG AGAATTGATT CACCAAGTGT GCTTTCGTTT 
CACCAGGTAC CATGA